LOCUS       DATLKL010000001         2367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_124032, whole genome shotgun sequence.
ACCESSION   DATLKL010000001 DATLKL010000000
VERSION     DATLKL010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124032"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VM713_00005"
     CDS             <1..392
                     /locus_tag="VM713_00005"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HVH82674.1"
                     /translation="TMAEQVVMSLDDHDRLIAYVLGLSHALNIAFFTALAESGEAAPK
                     LARMSSTTFDAQLDVAGRVAQDSPELYYEIQSLNDYGAESLEALSQAVERVRTAVLSQ
                     DHDTFVALMRRGRDYLEDRRTVTERRA"
     gene            404..1564
                     /locus_tag="VM713_00010"
     CDS             404..1564
                     /locus_tag="VM713_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HVH82675.1"
                     /translation="MSLPSTGPRRSAGSPDPTESLEQDNQALRARLASLTEEVSRNEG
                     LLRKSQERELELLRAGSLAQLFERLLVGLKTSYQLDGVMVVLNDPQHEIRHLISGDDL
                     AHGPQAGVMFVDALMTVAPQLATLERPWLGPYQRADHELLLPGQDHPASLALIPLRRH
                     DELDGVLVFMSNDQFRFTRELASDFLAHLGLVAAICVENAVNRARLLRSGLTDFLTGF
                     HNRRYLHARLREELARAQRARLPIVCLMIDVDHFKRINDQYGHLAGDAVLCEVAQRID
                     AEMRISDTGARFGGDEFAMVLSQADIGDGERVAKRVLSAVRQQPITIGSGANAASETV
                     TLSIGVAAANPGPGPHDYKALAERLIAEADATLYRAKSAGRNRVATSPNIVV"
     gene            1690..2247
                     /locus_tag="VM713_00015"
     CDS             1690..2247
                     /locus_tag="VM713_00015"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HVH82676.1"
                     /translation="MHPPRKHARLLVAGAAGLALPLLAAAGDPQFRLPSFPGLREQAT
                     ESVDVDLGWLPLYVTSWLMDAGDQEAAAVGKTIRSLKSVQIRSYRFDSEFTYPQAEID
                     QLRAQLAQPGWSPLVAVRKHGDAAGKDRENVDIYVALEDKKVKGLVIIACEARELTVV
                     NLVGTIDLDQIASLRKTFVADRSLK"
BASE COUNT          395 a          819 c          794 g          359 t
ORIGIN      
        1 ccaccatggc cgagcaggtg gtgatgagtc tcgacgatca cgatcggctc atcgcctacg
       61 tgctcggcct ctcgcacgcg ctcaacatcg ccttcttcac cgcgctggcc gagagcggcg
      121 aggcggcacc gaagctcgcc cgcatgtcga gcacgacctt cgatgcgcag ctcgacgtgg
      181 ccggacgcgt cgcccaggac agtccggagc tgtattatga aatccagagt ctcaacgatt
      241 acggcgccga gtcgctcgag gccctctccc aggcggtcga gcgcgtccgc actgcggtgc
      301 tctcgcagga tcacgatacc ttcgttgctt tgatgcgccg cgggcgcgat tatctcgagg
      361 acaggcgcac ggtcacggag cgccgggcct gagggaccag gagatgagcc taccgagcac
      421 cgggccgcgc cgcagcgccg gaagccccga tccaaccgag tcgctcgagc aggataacca
      481 ggcgctccgg gcgcgcctgg cgtcgctcac cgaggaggtg agccgcaacg aggggttgct
      541 gcgcaagtcg caggagcgcg agctcgagct gctgcgcgcc ggctcgctgg cgcagctgtt
      601 cgagcggctg ctcgtcggcc tcaagacctc ctaccagctc gacggcgtca tggtggtact
      661 caacgacccg cagcacgaga tccgccacct gatctccggc gatgatctcg cccacggccc
      721 gcaggcgggc gtcatgttcg tcgacgcgct gatgaccgtg gcgccgcagc tcgccacgct
      781 cgagcggccc tggctcggac cctaccagcg cgccgatcat gagctgctgc tgcccgggca
      841 ggaccatccg gcgagcctcg cgctcattcc gctgcgccgg cacgacgagc tcgacggcgt
      901 gctggttttc atgagcaacg accagttccg cttcaccagg gagctcgcca gcgacttcct
      961 ggcgcacctg ggcctggtgg cggccatctg cgtggaaaac gccgtcaatc gcgcgcgtct
     1021 gctgcgcagc ggactcaccg atttcctcac cggctttcac aaccgccgct acctgcacgc
     1081 ccggctgcgc gaggagctgg cgcgcgcgca gcgcgcccgg ctgccgatcg tctgcctgat
     1141 gatcgacgtc gatcacttca agcgcatcaa cgaccagtac ggccacctgg ccggcgatgc
     1201 ggtgctgtgc gaggtggcgc agcggatcga cgccgagatg cgcatcagcg acaccggcgc
     1261 gcgcttcggc ggtgatgagt tcgcgatggt gctgtcgcag gcggacatcg gcgacggcga
     1321 gcgggtcgcc aagcgggtgc tgagcgcggt gcgccagcag ccgatcacga tcggctccgg
     1381 cgccaatgcg gccagcgaga ccgtgacact ctcgatcgga gtcgcggccg caaaccccgg
     1441 ccccggcccg catgactaca aggcactcgc cgagcggctg atcgccgagg ccgacgcaac
     1501 gctctaccgc gccaagagcg cggggcgcaa ccgtgtcgcg acctcgccaa atatcgtcgt
     1561 ttagccaaac gacccagcct gtccggggcg cgctggcgct gccgcgcgct cccggctgca
     1621 ccctgcggac acccctcgtg cgtattaaca ctatggagag caaccaatcg aaaccggagg
     1681 cgctcgccta tgcacccccc tcgcaagcac gcacgactgc tggtggccgg cgccgccggc
     1741 ctcgcgctgc cgctgttggc ggcagccgga gatccgcagt ttcggctgcc gtcgttcccg
     1801 ggcctcaggg agcaggcgac cgaatcggtc gacgtcgacc tcggctggct gccactgtat
     1861 gtgacatcgt ggctgatgga cgcgggcgac caggaggccg ccgcggtcgg caagaccatc
     1921 aggagtctga agtccgtgca gattcgcagc taccgcttcg actccgagtt cacctacccg
     1981 caggcggaga tcgaccagct gcgggcgcag ctcgcgcagc ccggctggag cccgctggtg
     2041 gcggtacgca agcacggcga cgcggccggc aaggaccggg agaatgtcga catttacgtc
     2101 gccctggagg ataagaaggt gaagggactc gtcatcatcg cctgcgaggc ccgcgagctc
     2161 acggtagtca acctcgtcgg cacgatagat ctggatcaga ttgcgagcct gcgcaagacc
     2221 ttcgtggccg atcgaagcct gaagtgacgc gaacccaatg tgagcgggga cgaggagtgt
     2281 acgagcggcg atttgctcac gtgggctttg gccggcgagc aggcggcgtt cggcgccctg
     2341 gtgcggcgcc atcagcgctc ggtctac
//
LOCUS       DATLKL010000002         1560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_22561, whole genome shotgun sequence.
ACCESSION   DATLKL010000002 DATLKL010000000
VERSION     DATLKL010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22561"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..832)
                     /locus_tag="VM713_00020"
     CDS             complement(35..832)
                     /locus_tag="VM713_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HVH82677.1"
                     /translation="MGFLAGKRAFIVGVATDRSIAWGIAQAMHREGAELAFSYVNDKM
                     KERVEPLAHSLGSRLTMPLDVTVDGQIDAAYELLKREWGALDIVVHAVAFAPREALTG
                     TFVAATSREGFRVAHDVSSYSLTALARGAQPLMAGRAGALLTLTYLGAMRSIPGYNVM
                     GLAKASLEADVRFLAADLGPQGIRVNGISAGPIKTLAAAGIPGFRKMLSRVAEIAPLR
                     RNVTLEDVGNTAAFLCSDLAAGITGEILYVDGGFNSVGMSFPQEAEG"
     gene            complement(911..>1560)
                     /locus_tag="VM713_00025"
     CDS             complement(911..>1560)
                     /locus_tag="VM713_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HVH82678.1"
                     /translation="ATDPVGPYYLLLPVDAADVVTQNIAQLSADQLLGVTKYTVKRGD
                     SVASVAQRFHTTTNVLRELNDLPEGRLTVGDELKVPADSELPAKVVLAAARVDGKVRG
                     HRPRVFVVRNGDTLYSIARRNHVDVGTLAAMNDMRPGDALRPGQRIKVASSGSSRHGR
                     SHRHVLYTVRSGDTVAAIAQLFQCSVPQIVAWNGLGAHPHIHAGQKLRIHVIRHT"
BASE COUNT          259 a          511 c          532 g          258 t
ORIGIN      
        1 cgtttgatca atagccgcag gcgcgagcgt cgcgctagcc ttcagcttcc tgcggaaagc
       61 tcatcccgac gctgttgaag ccgccgtcga cgtacagaat ctcgccggtg atcccggcgg
      121 cgagatccga gcacagaaac gcggccgtgt tgcccacgtc ctcgagcgtc acattgcgac
      181 gcagcggggc aatctccgcc acgcggctca acatcttgcg aaagccggga atgccggcgg
      241 cggcgagcgt cttgatgggt cccgcggaaa tcccgttgac gcggatccct tgcgggccta
      301 agtccgcggc caggaagcgc acgtcggcct cgaggctcgc cttggcaagc cccatgacgt
      361 tatagccggg aatcgagcgc atcgcgccga ggtaggtgag ggtcaagagg gccccggcgc
      421 gtcccgccat cagcggctgc gcgccgcgcg ccagggcagt gaggctgtag ctcgacacat
      481 cgtgcgccac gcgaaagccc tcgcggctgg tcgcggcgac gaaggttccg gtgagcgcct
      541 cgcgcggagc gaacgccacc gcatgcacga cgatgtcgag ggcgccccac tcgcgtttca
      601 agagctcgta cgcggcatcg atctgcccgt cgacagtgac atcgagtggc atggtgagac
      661 gcgagccgag cgagtgcgcg agcggctcga ctctttcctt catcttgtcg ttcacgtagg
      721 agaacgcgag ctccgcgcct tcgcggtgca tggcctgtgc gatgccccag gcgatcgagc
      781 gatcggtcgc gacgcccacg atgaaggcgc gcttgccggc gagaaatccc atgttgagtc
      841 ctcccggctt cggattatgc cggaagtcgc gcgctgaccc gaacctcggc ccgggccagc
      901 ccgctttagc tcaggtgtgc cggatcacgt gaatgcgcag tttctggcct gcgtgaatgt
      961 gcgggtgggc acctaagccg ttccacgcca cgatctgcgg cacgctgcac tggaagagct
     1021 gcgcgatcgc agccacggtg tcgccgcttc ggaccgtgta gaggacatgc cgatgcgagc
     1081 ggccatgacg ggaggagccg ctgctcgcga ccttgatcct ctgccccggt cgcagcgcgt
     1141 ctccgggccg catgtcgttc atcgcggcca gcgtgcccac gtccacgtgg ttgcggcgcg
     1201 cgatggagta gagcgtatct ccgttgcgca ccacgaacac acgggggcgg tggccgcgga
     1261 ctttgccatc gacgcgtgcg gcggcgagca ccaccttggc gggaagctca gagtccgcgg
     1321 gcaccttgag ctcatcaccg accgtgaggc gcccttccgg caggtcgttg agctcccgga
     1381 ggacgttcgt cgtggtatgg aatcgttgcg cgacagaggc gacggaatcg ccgcgcttca
     1441 cggtgtactt agtcacgccg aggagctgat ccgccgagag ctgcgcgatg ttctgggtga
     1501 cgacatccgc agcgtccacc ggcaggagca ggtaataagg cccgaccggg tcggtcgccc
//
LOCUS       DATLKL010000003         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_11312, whole genome shotgun sequence.
ACCESSION   DATLKL010000003 DATLKL010000000
VERSION     DATLKL010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11312"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1961
                     /locus_tag="VM713_00030"
     CDS             168..1961
                     /locus_tag="VM713_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HVH82679.1"
                     /translation="MAANPTLLQSPKDFPCTEELTRINESLQGRERLLTATARASRLL
                     LEADNVRGAIPAVLGLIGEAAHVDRVNLMETRTGLSGEPLLVVVSEWTAEGVAPSLHD
                     PVSCECDERNFSAVCAELRAGRSVCFSPDETPDGRTAPFQGIGTRSKAIVPIFVAGEF
                     IGLVGFDNTRQRRAIDAAELAALETSAGVIGAALHRERLVDDVRHEREHSAEERFAEL
                     AKANAVIRGNLERLAGETDLHSFMGHMLLEATRQFDAISGAVIVLKDSLQEWRIIAHV
                     RDGQLAEPSFAVKVPVAGSIFTERFGQLREPHYLDVAAQPEIWPGVQAFHRSEGAVGT
                     VFYPLVFGARSVGFFILTFRRNAEDVKGSGLLVALAQQATLAVQLTRLAYSAKEAAVL
                     MERTRIGQEIHDGLAQAFTGILMQLGAAEDFPPCKKRNSELAITLSRIRDLARDGLAE
                     ARRSVMALRLDQTRRAGLELALRQLAERSTVPGGVTCTFDGGGVATGLRPEHEHELLR
                     IAQEAVSNAVRHARPQNVRITTAEDSAHWVMTIADDGVGMEQRPELYARQGFGLSSMR
                     QRAGAIGGEWQIESQPGAGTRVSVRMAKRAA"
BASE COUNT          317 a          702 c          707 g          304 t
ORIGIN      
        1 ttgattcttg agcccgttcc gaggccgccg ctgtgccgcg cgctgccaag ttgctccctc
       61 taaagagtga cgcccgtgcc tgcctgtggg gcaccggcag gtggaaggga cccgccgcgc
      121 gctaaactct tgggtcaact ccactggaag cccggacact caagctcatg gccgccaatc
      181 cgaccctgct tcaatcgccc aaggacttcc cgtgcacgga agagctgacc cggatcaacg
      241 agtcgttgca aggacgggag cggctgctga ccgccacggc gcgggcgagc cgcctactgc
      301 tcgaggcgga taacgtgcgc ggcgcgattc cggcggtcct cgggttgatc ggcgaagccg
      361 cgcatgtcga ccgcgtcaat ctcatggaga cccgcaccgg tctgagcggc gagccgctgc
      421 tcgtggtggt gagcgagtgg accgccgagg gcgtggcccc gagtctccac gatccggtga
      481 gctgcgagtg cgacgagcgc aatttctccg ccgtgtgcgc cgagctgcgc gcggggcgta
      541 gcgtgtgctt cagccccgac gagacgcctg acgggcgtac ggcacccttc caggggatcg
      601 gcacgcgctc caaggcgatc gtacccatct tcgtcgccgg cgagttcatc gggctggtgg
      661 gcttcgacaa tacgcgccaa cgccgcgcca tcgacgccgc cgagctggcc gcgctcgaga
      721 cctcggccgg cgtcattggc gccgcgctgc atcgcgagcg gctggtggac gacgtccgtc
      781 atgagcgcga gcactcggcg gaggagcgct tcgccgagct cgccaaagcc aacgccgtga
      841 tccgcggcaa cctcgagcgc ctcgccggcg agaccgatct gcacagcttc atggggcaca
      901 tgctgctcga ggcgacgcgc cagttcgacg cgatctcggg agccgtcatc gtcctcaagg
      961 actcgctgca ggagtggcgc atcatcgcac acgtgcgcga cggacaactc gccgagccct
     1021 cgttcgcggt caaggttccc gtggccggct cgatcttcac cgagagattc ggccagctgc
     1081 gtgagccgca ctacctcgac gtcgcggccc agccggagat ctggccgggg gtgcaggctt
     1141 ttcaccgtag tgaaggggcg gtcggcacgg tgttctatcc gctcgtgttc ggcgcacgca
     1201 gtgttggttt cttcatcctg acgttccgcc gcaatgccga ggacgtgaag ggcagcggcc
     1261 tgctcgtggc gctcgcccag caggcaacgc tcgccgtgca gctcacgcgc ctcgcctatt
     1321 ccgccaagga agcggccgtg ctcatggagc gcacgcgcat cgggcaggag attcacgacg
     1381 gcctggcaca ggccttcacc ggcatcctga tgcagctcgg cgccgccgag gacttccccc
     1441 cctgcaagaa gcgcaactcc gagcttgcca tcacgctgtc ccggatccgc gacctggcgc
     1501 gcgacggact cgccgaggcg cgccgctcgg tcatggcgct gcgcctcgac cagacgcgcc
     1561 gcgcggggct cgagctggcg ttgcgccagc tggccgagcg ctccaccgtg ccgggcggcg
     1621 tcacctgcac gttcgacggc ggcggcgtgg ccaccgggct gcgccccgag catgagcacg
     1681 agctgctgcg catcgcgcag gaggcggtga gcaacgcggt gcgccacgcg cgcccgcaga
     1741 acgtgcgcat caccaccgcc gaggattcgg cacactgggt catgaccatc gccgacgacg
     1801 gcgtcgggat ggagcagcgg cccgagctct acgcacggca gggtttcgga ctgtcgagca
     1861 tgcgccagcg cgccggggcc atcggcgggg agtggcagat cgagagccag cccggcgcag
     1921 gcacgcgggt gagcgtgcgc atggcaaagc gggcggcctg atggcagcgg aactgaaagc
     1981 gcgccggatt agcgtcatcc tgccggacga tcatccggtg gtgcgcgacg
//
LOCUS       DATLKL010000004         1520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_146658, whole genome shotgun sequence.
ACCESSION   DATLKL010000004 DATLKL010000000
VERSION     DATLKL010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146658"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /gene="lpxD"
                     /locus_tag="VM713_00035"
     CDS             complement(<1..883)
                     /gene="lpxD"
                     /locus_tag="VM713_00035"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HVH82680.1"
                     /translation="MSVSLGELAVRFGCELRGDPEARVAHVATLSNADATALAFLANP
                     RYRPLLASTRAAAVVLKPEAAADCPTHVLLAENPYATYARIAAVLHPSPAAPPGVHPT
                     ALIAASARIDPSAHIGAFCSIGERTVVGERCVVGPHCVLEEDVKLAPDVRLAVRVTVH
                     RGVEVGARTVLQSGAVIGGDGFGYAPERGTWVKVPQVGAVRVGADVEIGCNTTIDRGA
                     IEDTVIEDGVKLDNLIQIGHNVRIGAHTAIAACTGVSGSTIIGKRCMIGGHVGFAGHI
                     TIADDVVITGFSAVSRSI"
     gene            complement(918..>1520)
                     /locus_tag="VM713_00040"
     CDS             complement(918..>1520)
                     /locus_tag="VM713_00040"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HVH82681.1"
                     /translation="CSVNSSISSRMAVWTVAALGALAALPAWAEGKLAVVDFQRLAQE
                     SPQGKAVMDAMRTEFAPRERTLQAQMQSLKAKQDKLQKDGATMTDDQRARAEKELRDG
                     ERDFERARGELQDDVTARRNEEMSRLQRTLVEEVRGYAKTQNLDMVVSGEAVLYAAPA
                     FDITPAVLTALQAHAAAAPAAKPAAPAATPAPVPAPKQNK"
BASE COUNT          210 a          562 c          520 g          228 t
ORIGIN      
        1 cgatcgagcg gctcaccgcc gagaagcccg tgatgacgac gtcgtcagca atcgtgatgt
       61 gccccgcgaa gcccacgtgg ccgccgatca tgcagcgctt gccgatgata gtgctgccag
      121 aaacaccggt gcaggcggcg atcgcggtgt gggcgccgat gcgcacgttg tgtccgatct
      181 ggatgaggtt gtcgagcttc acgccgtcct cgatcacggt gtcctcgatg gcgccgcggt
      241 cgatggtggt gttgcagccg atctccacgt ccgcgccgac gcgcaccgcg ccgacctgcg
      301 gcactttcac ccaggtgccg cgctcgggcg cgtagccgaa gccgtcgccg ccgatcacgg
      361 cgcccgactg cagcacggtg cgcgcgccga cctcgacccc gcgatgcacc gtgacgcgca
      421 ccgccaggcg cacgtcgggc gcgagtttca catcctcttc cagcacgcag tgcggaccca
      481 cgacgcagcg ctcgccgact acggtgcgct cgccgatcga gcagaaggcg ccgatgtgcg
      541 cggatggatc gatgcgggcg ctcgcggcga tgagcgccgt cgggtgcacg ccgggaggcg
      601 ccgccgggct cggatgcagc accgcggcga tgcgcgcata agtcgcgtag ggattctcgg
      661 cgagcagcac gtgcgtcggg cagtccgcgg ccgcctcggg cttgaggacc accgccgcgg
      721 cgcgcgtgct ggcgagcagc ggccggtagc gggggttcgc gaggaacgcg agcgccgtcg
      781 cgtcggcatt cgagagcgtc gcgacgtgcg cgacgcgcgc ctccgggtca ccccgcagct
      841 cgcagccgaa gcgcaccgcg agctcgccca gcgagacgga catggcgcga gtcgaccccc
      901 gggagctgcc tcggacgcta tttgttctgc ttgggcgccg gcaccggtgc cggagtcgcc
      961 gcaggagccg cgggcttggc cgccggcgcc gcggcggcgt gcgcctgcag ggcggtgagt
     1021 accgccggcg tgatatcgaa ggccggggcc gcgtacagca ccgcctcgcc gctcaccacc
     1081 atgtcgaggt tctgcgtttt ggcgtagccg cgtacctcct cgaccagcgt gcgctgcagc
     1141 cgcgacatct cctcgttgcg ccgcgcggtc acatcatcct gcagctcgcc gcgcgcgcgc
     1201 tcgaagtcgc gctcgccgtc gcgcagctcc ttttccgcgc gggcccgctg atcgtcagtc
     1261 atggtcgcgc cgtccttctg cagcttgtcc tgcttggcct tcagcgactg catctgcgcc
     1321 tgcagcgtgc gctcgcgcgg cgcgaactcc gtgcgcatcg cgtccatgac cgccttaccc
     1381 tgcggagact cctgcgccag ccgctggaaa tccaccaccg ccaactttcc ctcggcccat
     1441 gccggcagcg cggccagcgc ccccagcgcc gccacggtcc aaaccgccat ccgactgctg
     1501 atcgaagaat tcacggaaca
//
LOCUS       DATLKL010000005         2013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_191757, whole genome shotgun sequence.
ACCESSION   DATLKL010000005 DATLKL010000000
VERSION     DATLKL010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191757"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VM713_00045"
     CDS             <1..252
                     /locus_tag="VM713_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82682.1"
                     /translation="LLRQGYFADIVVFDPAKVQDHSTYQRPQQLATGVGDVWVNGVRA
                     LRHEVATAAASGRALRGRAWTGAGGGGCRNSAADWTWSP"
     gene            complement(244..1500)
                     /locus_tag="VM713_00050"
     CDS             complement(244..1500)
                     /locus_tag="VM713_00050"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVH82683.1"
                     /translation="MLLYHKREHSVPYSYATHLECSATGERHDIGRLQGLSVAGKPLL
                     ARYDLAALRRERSRADIENRAPGLWRWQELLPVPQPEHIVSLGEVETPLIELVKSERP
                     VGFLPPLVKDEGRLPTGSFKARGLALAVSMAQSFGVRRVAIPTNGNAGAAMAAYAARA
                     SMEAFCFCPDDTPEVNLSEIALQGATVWRVNGLINDCARLVALGKERFGWFDMSTLKE
                     PYRIEGKKTMGLELAAQMQWRLPDVVFYPTGGGTGLIDMWKGWQELTALGWIEGPMPR
                     LVAVQSTGCAPIVRAFEAGAETATPWENAHTIASGIRVPAALGDFLILRALRDTSGFA
                     VAVEDADIERARSEVAANDGLLLSPEGAATYAAYLAATRAGKLRASDRVVLFNCASGL
                     KYPMPPVTARMDLRRPFADSPPTASG"
     gene            1502..>2013
                     /locus_tag="VM713_00055"
     CDS             1502..>2013
                     /locus_tag="VM713_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVH82684.1"
                     /translation="MSQGPSLSAPQLVRAIGRWSMVALAVNCIIGSGIFGLPSVVAGY
                     LGGWSPVAVLLAGLAMAAIIGCYAEAASQFTGTGGHYLYVRRAFGRFAGVQVGWMTLL
                     SRLTACAAAVNLLVISLKEFWPGAGAPMPRFAVVSLFVGSLGLANYRGVATGTLVSNV
                     SVVAKLVTLAL"
BASE COUNT          286 a          672 c          723 g          332 t
ORIGIN      
        1 ctgctgcgcc agggttactt cgccgatatc gtggtgttcg atccggcgaa ggtccaggac
       61 cactccacct atcagcgtcc gcagcagctc gcgacgggcg tcggcgatgt atgggtcaac
      121 ggcgtgcgcg cgctgcgcca cgaggttgcg accgccgcgg cgagcggccg cgcgctccgc
      181 ggtcgcgcct ggacgggggc aggcggtggc ggctgtcgca atagtgccgc tgactggacc
      241 tggtcaccct gacgctgtcg gcggcgagtc ggcgaagggc cggcgcaggt ccatgcgggc
      301 ggtcacgggc ggcatggggt atttgaggcc actcgcgcag ttgaacagga cgacgcggtc
      361 gctcgcgcgg agcttgcccg cgcgtgtcgc ggcgaggtac gccgcatagg tcgcggcgcc
      421 ttcgggactc aagagcaacc catcattcgc cgcgacttcg ctgcgggcgc gctcgatgtc
      481 ggcgtcctcg accgcgacgg cgaagccgct ggtgtctcgg agcgcccgga ggatcaggaa
      541 atcgccgagc gccgctggca cgcggatccc cgaggcgatg gtgtgcgcgt tctcccaggg
      601 agtggcggtc tccgcgcccg cctcaaaggc gcgcacgatc ggcgcgcaac ccgtggactg
      661 caccgcgacc aggcgcggca tcggaccctc gatccagccg agcgcggtga gctcctgcca
      721 tcccttccac atgtcgatga ggccggtgcc gccgcccgtc gggtagaaca ccacatccgg
      781 cagccgccac tgcatctggg cggcaagctc gagtcccatg gttttcttgc cctcgatccg
      841 gtagggctcc ttgagcgtcg acatatcgaa ccagccgaag cgctccttgc ctaaggccac
      901 gagacgggcg cagtcgttga tgagcccgtt gactcgccac acggtcgcgc cctgcagcgc
      961 gatctcgctc aagttgactt ccggggtgtc gtccggacag aagcagaatg cttccatgct
     1021 cgcgcgggcc gcgtaagcgg ccatggcggc gccggcgttg ccgttggtcg gaatcgcgac
     1081 gcggcggacg ccgaaggact gcgccatcga gacggcgaga gccaggccgc gtgccttgaa
     1141 cgagccggtc ggcaggcgcc cctcatcctt gacgagcggg ggcaggaagc ccacgggccg
     1201 ctcgctcttt acgagctcga tgagcggggt ctccacctca ccgaggctca cgatgtgctc
     1261 aggctgcggc accggcagga gctcctgcca gcgccagagg ccaggcgcgc ggttctcgat
     1321 gtccgcgcgc gagcgctcgc gtcgcaacgc cgcgaggtcg taacgggcga ggagcggctt
     1381 gcctgcgacc gacagtccct gcagccggcc gatgtcatgt cgctcgccgg tcgcgctgca
     1441 ttcgagatgg gtcgcgtagg agtagggcac cgagtgctcc cgcttgtgat acaacagcac
     1501 gatgagccag gggccatcgc tcagcgcgcc gcaactggtc cgcgccatcg gccgctggag
     1561 catggtagca ctggcggtga actgcattat cggcagcggc atcttcggat tgccgtcggt
     1621 ggtggcgggc tacctcggcg gatggagtcc ggtggcggtg ctgttggcgg ggctggcgat
     1681 ggcggcaatc atcggctgct acgccgaggc ggcctcgcag ttcaccggga ccggcgggca
     1741 ctatttatat gtgcggcgcg ccttcgggcg cttcgccggc gtgcaggtcg gctggatgac
     1801 gctgctcagc cgcctcaccg cctgcgccgc cgctgtcaac ctgttggtca tctcgctcaa
     1861 ggagttctgg ccgggggcag gcgcacccat gccgcgcttc gcggtggtgt cgctgttcgt
     1921 gggatccctc gggctcgcga actatcgtgg cgtcgccacc ggcacgttgg tgagcaacgt
     1981 gtcggtggtc gccaagctcg tgacgctcgc cct
//
LOCUS       DATLKL010000006         1735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_101578, whole genome shotgun sequence.
ACCESSION   DATLKL010000006 DATLKL010000000
VERSION     DATLKL010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101578"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..804
                     /locus_tag="VM713_00060"
     CDS             130..804
                     /locus_tag="VM713_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVH82685.1"
                     /translation="MLLVEDEKKVSELVARALRQESYAVDVADDGTRGWELVQAYDYD
                     LIILDLMLPGLPGEELLRRIRRRNQQVPILILTARAATEDKVHNFEAGADDYLTKPFA
                     FAELVMRVKALLRRGPVARSSVLRVGDLEVDRFTKQVRRGGRRIELTPKEYALLEYLA
                     SNPGRVFSRTMIIEHVWDQSFEGLTNIVDVYVRHLRSKVDDPFPVKLIRTVRGVGYGL
                     GSGAEP"
     gene            801..>1735
                     /locus_tag="VM713_00065"
     CDS             801..>1735
                     /locus_tag="VM713_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVH82686.1"
                     /translation="MNARSLSFRLVSWYAGVLTAVFVLLGLLTFLLLRHYLEANLLDA
                     QSRRARQIAGTLVAQAHRETEEAMRSEVERLYSPEVNDRFIRISRADGHVVYASGTPR
                     NEHFDPGRVPAAGGNFLEAATREVPLPGGALLIAAVPATGSDGTLYRVEVGTAAAAAE
                     GTLAQVLVMLAVGLPVAVAVAVAGGFILVRQALEPVERMAAKAEEITQQNLSERLPVV
                     ESGDELERLSVALNHMISRLEDALRGSKQFVADASHELRTPLTVMRGELESLAQDALL
                     TRDTREALGSVLEEVERLAEIVESLFALSRLDAGEA"
BASE COUNT          267 a          569 c          607 g          292 t
ORIGIN      
        1 atatcaacgt cttaaatgca acttctattt ctctgaaggg tttttaatgc ggcgtttgcc
       61 gtcgattaat ggccctgtgc tcaactgagc tgcatccatt cacgccgcgg aggcttagcc
      121 gtcgtgcgca tgctcctcgt cgaggacgag aagaaggtct ccgagctcgt ggcgcgcgcg
      181 ctgcggcagg aaagctacgc cgtcgacgtc gccgacgacg gcacacgcgg ctgggagctc
      241 gtccaggcgt acgactacga tctcatcatt ctcgacctga tgctccccgg gctcccgggc
      301 gaggagctgc tgaggcgcat ccggcgccgc aaccaacagg tgccgatcct gatcctcacc
      361 gcgcgcgctg cgaccgagga caaggtccat aatttcgagg cgggagccga cgactatctc
      421 accaagccct tcgccttcgc cgagctcgtg atgcgcgtca aggcgctgct gcggcgcggt
      481 ccggtcgctc gctcgagcgt gctgcgggtc ggagatctcg aggtcgaccg cttcaccaag
      541 caggtgcgcc gcggcgggcg gaggatcgag ctcacgccca aggaatacgc gctgctcgag
      601 tacctggcgt cgaatcccgg acgcgtgttc tcccgtacga tgatcatcga gcacgtgtgg
      661 gaccagagct tcgagggcct gacgaacatc gtcgatgtgt acgtacggca cctgcgcagc
      721 aaagtcgatg acccgttccc ggtgaagctg atccgcacgg tgcgcggtgt gggctacggc
      781 ctcgggtcgg gcgccgagcc ttgaacgcgc gctccctgag ctttcgtctg gtctcctggt
      841 acgcgggggt cctcacggcc gtgttcgtgc tgctcggcct gctcaccttc ctgctgctgc
      901 ggcactacct cgaggcgaac ctgctcgacg cccagagccg ccgcgcccgg cagatcgccg
      961 ggacgctggt cgcccaggcg caccgcgaga ccgaggaggc gatgcgcagc gaggtcgaga
     1021 ggctctattc gcccgaggtt aacgatcgct tcatccgcat ctcgcgggcc gacgggcacg
     1081 tcgtctacgc atcggggacg ccgcgcaacg agcacttcga tcccgggcgc gtgccagcgg
     1141 ccggcggtaa ttttctcgag gccgccacgc gcgaggtgcc gctgccgggc ggcgcgctgc
     1201 tgatcgcggc cgtgccggcg acggggagcg atggcacgct ctaccgggtc gaggtcggca
     1261 ccgccgcagc agcggccgag ggcacgctgg cacaggtgct cgtcatgctg gccgttgggc
     1321 tgccggtcgc ggtcgcggtg gcggttgccg gcgggttcat cctggtacgg caggcgctcg
     1381 agccggtcga gcgcatggct gcgaaggctg aggagatcac ccagcagaac ctgagcgagc
     1441 gcttgcccgt ggtggagagc ggcgacgagc tcgagcggct ctcggtcgcg ctcaatcaca
     1501 tgatcagccg cctcgaggat gcgttgcgcg gctcgaagca gttcgtggcg gatgcgagcc
     1561 acgagctgcg cacgccgctc acggtgatgc ggggcgagct cgagagcctg gcacaggacg
     1621 cgcttctcac gcgcgacacg cgcgaggccc tgggaagcgt gctcgaggag gtcgagcgcc
     1681 tcgccgagat cgtcgagagc ctgttcgcgc tgtcgcgcct cgatgccggc gaggc
//
LOCUS       DATLKL010000007         3132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_112846, whole genome shotgun sequence.
ACCESSION   DATLKL010000007 DATLKL010000000
VERSION     DATLKL010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112846"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VM713_00070"
     CDS             <1..1019
                     /locus_tag="VM713_00070"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HVH82687.1"
                     /translation="HGWSYLRGEFGEFVEVVPAQQQLGAVWYRGTADAVHQNIELILA
                     HRPKHVLVLAGDHIYKMDYGPMIAYHVEKGADITVGVVEVSADQSRHYGVLTATEWNR
                     VTRFAEKPAVPDPLPGRPDTILASMGIYVFNAPLLESVLTADAAREASSHDFGKDILP
                     EAISSGRQVFAYPFQDVKTRAQSYWRDVGTLDAYYDANVELVHVRPELNIYDEDWPIW
                     TYQVQQPPAKFILDEDGRRGMAINSMVSGGCIISGAVVRESLLFSDVRVEERTTIHRS
                     VVLPHAEIGRGCSISGAIIDESCEVPDGMSIGSDRAADEQRFHVTEKGVVLVTPDMLR
                     KLRS"
     gene            1032..2735
                     /locus_tag="VM713_00075"
     CDS             1032..2735
                     /locus_tag="VM713_00075"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HVH82688.1"
                     /translation="MAPAARLPVVLLWHMHQPQYRDALTEEYTLPWTYLHAIKDYTDM
                     AAHLEANPAARAVVNFSPVLLEQLEDIAARIAAHLKSGAPLPDPVLALLGPDPLPAEG
                     APRLALLNACLRAQRKQMIERFGPYLELATIADTLATAERVGYASDHFIHDLAVWYHL
                     AWLGETVRRTDPLVAQLTARGRNFSDTQRRELLALIGDLVARVIPRFRALGERGQCEL
                     SVTPYAHPIIPLLLDFRAARDTVPAMVLPEHAAYPGGAARAEWHVAEAIRVFTRVFGA
                     RPAGCWPAEGAVSAATLALLGAQGFRWAASGGAVLHNSLELTDPQAALEPRSYNRPYR
                     PPGTGIDCFFRDDNLSDLIGFTYATWHGDDAAHNLVNELTQLAQRYDGDPNHAVLIAL
                     DGENAWEHYPFNGYYFLKALYALLASHPLIELTTLSGCLARGLQAAPLPRLAAGSWVH
                     GTLATWMGDPDKNRAWDLLCEAKQVFDRAVASPAFDTAAREPTGRQLALCESSDWFWW
                     FGDYNPAESVSQFDQLYRRQLVTLYRRLGLEAPAMLAQPISTGAGSPEHGGVMRRASA
                     G"
     gene            2750..>3132
                     /locus_tag="VM713_00080"
     CDS             2750..>3132
                     /locus_tag="VM713_00080"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HVH82689.1"
                     /translation="MAGMRLPVFDRRRAGALLPLTALEAPLGRGGRAFVDWLADAGFS
                     VWQILPVGPTGADGSPYWVRSDWAGNAQYLDPGELPAAATAPAAEFRADSAAWLPDYA
                     LFEALTRAHDGAPFWQWPAELRDRAS"
BASE COUNT          460 a         1148 c         1052 g          472 t
ORIGIN      
        1 aacacggctg gagttacctg cgcggcgagt tcggtgagtt cgtcgaagta gtgccggcgc
       61 agcagcagct cggcgccgtc tggtaccgcg gcaccgcgga cgcggtgcat cagaacatcg
      121 agctgattct cgcgcatcgt cccaagcacg tgctggtgct cgccggggac cacatctaca
      181 agatggacta cggcccgatg atcgcttacc acgtggagaa gggcgccgac atcaccgtcg
      241 gagtggtcga ggtgagcgcg gatcagtcgc gacactacgg cgtgctcacc gccaccgagt
      301 ggaaccgcgt gacccgcttc gccgagaagc cggccgtgcc cgaccccctg ccgggccgcc
      361 ccgacaccat cctcgcctcg atgggcatct acgtgttcaa cgcgccgctt ctggagagcg
      421 tgctgaccgc ggacgccgca cgcgaggcct cgtcccatga cttcggcaag gacatcctgc
      481 cggaggcgat cagcagcggc cgccaggtat tcgcctatcc cttccaggac gtgaagacgc
      541 gcgcgcagag ctactggcgc gacgtcggca cgctggatgc ctactacgac gccaatgtcg
      601 agctggtgca tgtgcggccc gagctcaaca tctacgacga ggactggccc atctggacct
      661 accaggtgca gcagcccccc gccaagttca tcctcgatga ggacggccgc cgcggcatgg
      721 cgatcaactc catggtgtcg ggcggctgca tcatctccgg cgccgtagtg cgcgagtcgc
      781 tgctgttctc ggacgtgcgc gtcgaggagc gcacgaccat ccatcgctcg gtggtgctgc
      841 cgcacgccga gatcggacgc ggctgcagca tcagtggcgc catcatcgac gagagctgcg
      901 aggtgcccga cggcatgagc atcggcagcg atcgcgccgc ggacgagcag cgctttcacg
      961 tcaccgagaa gggcgtggtg ctggtcacgc ccgacatgct gcgcaagctc cgctcctgag
     1021 gggacctatc ggtggcaccc gccgcgcgcc tgccggtcgt actgctgtgg cacatgcacc
     1081 agccgcagta ccgcgacgcg ctgacggagg agtacacgct cccatggacc tatctgcacg
     1141 ccatcaagga ctacaccgac atggcggcgc acctcgaggc gaaccccgcg gcgcgcgccg
     1201 tcgtcaactt ctcacccgtg ctcctcgagc agctcgagga catcgcggcg cggatcgccg
     1261 cgcacctgaa gagcggcgca ccgctgcccg atccggtact cgcgctgctc ggcccggacc
     1321 cgctgccggc ggagggcgcg ccgcgcctgg cgctcctcaa cgcctgcctg cgcgcgcagc
     1381 gcaagcagat gatcgagcgc ttcgggccct acctcgagct cgcgaccatc gccgacacgc
     1441 tcgcgaccgc ggagcgggtc ggttacgcct ccgatcactt catccacgat ctcgccgtct
     1501 ggtaccacct ggcgtggctc ggcgagacgg tgcgccgcac cgatcccctg gttgcgcagc
     1561 tcaccgcgcg cggccggaat ttcagcgaca cgcagcgccg cgagctcctc gcgctgatcg
     1621 gcgatctcgt ggcgcgcgtg ataccgcgct tccgcgcgct cggcgagcgg ggccagtgcg
     1681 agctgtcggt cacgccctac gcgcatccga tcattccact gctgctcgac ttccgtgcgg
     1741 cgcgcgatac cgtgcccgcc atggtgctgc ccgagcacgc ggcctatccg ggcggcgccg
     1801 cgcgcgccga gtggcacgtc gcggaggcga tccgggtatt cacccgcgtg ttcggcgcgc
     1861 gcccggcggg gtgctggccg gccgagggtg cggtcagcgc cgccacgctc gcgctcctcg
     1921 gcgcgcaggg cttccgctgg gccgcgagtg gcggagcggt gctccacaac agcctcgagc
     1981 tgaccgaccc gcaggccgcg ctcgagccgc gcagctacaa ccgcccgtac cgccctccgg
     2041 gcacaggtat cgactgcttc ttccgcgacg acaatctttc cgacctgatc ggcttcacct
     2101 acgcgacctg gcacggcgac gatgcggctc acaacctggt taacgagctg acgcagttgg
     2161 cgcaacgcta cgacggtgac ccgaaccacg cggtgctgat cgcgctcgac ggcgagaatg
     2221 cctgggagca ctacccgttc aacggctact acttcctcaa ggcgctctac gcgctgctcg
     2281 cgagccatcc gctcatcgag ctcacgacgc tctccgggtg cctcgcgcgc ggcctgcagg
     2341 cggcgccgct gccacgcctc gcagccggca gctgggtgca tggcacgctc gccacctgga
     2401 tgggcgaccc ggacaagaac cgcgcctggg acctgctgtg cgaggcgaaa caggtattcg
     2461 atcgcgccgt ggcgagtccg gcgttcgaca ccgccgcgcg cgagccgacc ggccggcagc
     2521 tggcgctgtg cgaaagctcc gactggttct ggtggttcgg ggactacaac cccgccgagt
     2581 cggtgagtca gttcgaccag ctgtatcgcc gccagctcgt cacgctctac cgccggctcg
     2641 ggctcgaggc cccggcgatg ctcgcgcagc cgatctccac cggcgcaggc tcgcccgagc
     2701 acggtggggt catgcgccgc gccagcgccg gctgagcggg taagggcgca tggccgggat
     2761 gcgcttgccc gtgttcgatc ggcggcgcgc cggggcgctg ctgccgctga cggcgctcga
     2821 ggcacccctc gggcgcggcg gacgcgcctt cgtcgactgg ttggccgacg ccggcttctc
     2881 cgtgtggcag atcctgccgg tggggccgac cggagctgac ggctcgccgt actgggtacg
     2941 ctccgactgg gccggcaacg cgcagtatct cgacccgggc gagctgcctg ccgcggccac
     3001 ggcgcccgcg gccgagtttc gcgcggactc cgccgcctgg cttcccgact acgcgctctt
     3061 cgaggcgctc acccgcgcac acgatggcgc gcctttctgg caatggcccg cggagctgcg
     3121 tgaccgcgcg tc
//
LOCUS       DATLKL010000008         2736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_67730, whole genome shotgun sequence.
ACCESSION   DATLKL010000008 DATLKL010000000
VERSION     DATLKL010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67730"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /gene="lnt"
                     /locus_tag="VM713_00085"
     CDS             complement(<1..1501)
                     /gene="lnt"
                     /locus_tag="VM713_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HVH82690.1"
                     /translation="MALKLLPRPSSPRLRALIALAAGAALAAAFAPHGLWPLAVLCPA
                     ALMWLWQDATPRESAWLGSLFNAGTFGVGTYWLLISLPIGGAPIWLALLLWLGLTGIM
                     GLYHAALGYGCARWLPHAGTVRSLVALPAAWLLVEWWRGWFLSGFSWLSLGYSQTDTW
                     LAGLAPLTGVYGVSAALLLSAGALIVLARGTKWARLSAAAVLLVPWAAGAALQGISWT
                     HPAGTAVSVAIAQGAVPQDEKWLDANREATLDLYQKLTEQALGTALIVWPESAPADTA
                     NDLVPWLTQIYRDAHARGSALVLGVLRAEGGATDSDPVRYYNSVLALDAQVDWYDKHH
                     LVPFAEFFPVPQFVRSWMRLASLPYSDFTRGAAYQPPLEAAHLKLGATVCYEDGYGSS
                     MLEVLRRGGADALVNVTNDAWFGHSSARHQHFQIARMRALEEGRYLIRAANDGISAVI
                     GPHGEVVARAAEFRPVVLVSAVTPLTGLTPYARVGNWLIVPLAALALAYG"
     gene            complement(1502..2374)
                     /locus_tag="VM713_00090"
                     /pseudo
     CDS             complement(1502..2374)
                     /locus_tag="VM713_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798742.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
     gene            complement(2371..>2736)
                     /gene="ybeY"
                     /locus_tag="VM713_00095"
     CDS             complement(2371..>2736)
                     /gene="ybeY"
                     /locus_tag="VM713_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HVH82691.1"
                     /translation="ANYFSVLGVMPVLGRSFTNLEETSNQPTNVLSFPPPAMPGGTQP
                     GARALGDLVICPGVLRGEARAQAKALKAHWAHLIVHGALHLIGYDHQGEADARRMERR
                     EIAVLRRLGFANPYLERPP"
BASE COUNT          460 a          962 c          954 g          360 t
ORIGIN      
        1 gtccgtatgc cagcgccagc gccgccagcg gcacgatcag ccagtttcca acacgcgcgt
       61 agggggtgag gccggtcagg ggggtcaccg ccgagaccag caccacgggg cggaattccg
      121 cggcccgggc cacgacttcg ccgtgcggcc cgatgaccgc ggaaatgccg tcgttggcgg
      181 cccggatcag gtagcggccc tcctccagcg cccgcatgcg cgcgatctgg aagtgctgat
      241 gacgggcgct cgagtggccg aaccaggcgt cgttggtgac gttcaccagc gcatcggcgc
      301 ccccccgccg cagcacctcg agcatggagc tgccgtagcc gtcctcatag cacaccgtcg
      361 cgccgagctt gaggtgcgcc gcctcgaggg gcggctgata ggcggcgccg cgggtgaagt
      421 ccgaatacgg caggctcgcg agccgcatcc aggaacgcac gaactgcggt accgggaaga
      481 attccgcgaa cggcaccaga tggtgcttgt cgtaccagtc cacctgagca tcgagcgcga
      541 gcaccgagtt gtagtagcgc acgggatccg agtcagtggc gccgccctcg gcgcgcagca
      601 cgcctaagac gagtgccgag ccgcgcgcgt gtgcgtcgcg gtagatctgc gtgagccagg
      661 gcacaagatc gttggccgta tcggccggcg ccgactcggg ccacacgatg agcgcggtac
      721 cgagggcctg ctcggtaagc ttctggtaga ggtcgagcgt cgcctcgcgg ttggcgtcga
      781 gccatttctc atcctgcggg actgccccct gagcgatcgc caccgacacc gcggtcccag
      841 cggggtgtgt ccaggagatg ccctgcagcg ccgcccccgc ggcccaggga acgagcagca
      901 cggccgccgc cgagagccgc gcccatttcg tgccgcgcgc gaggacgatg agcgctccgg
      961 cggacagcag caaggccgcg ctcacgccat acactccggt aaggggcgca aggcccgcga
     1021 gccaggtgtc ggtctgcgag tagccgagcg acagccacga gaaacccgac aggaaccagc
     1081 cgcgccacca ctcgacgagc aaccacgccg cgggcagtgc cacgagcgag cgcaccgtcc
     1141 ccgcgtgcgg cagccagcgc gcgcagccgt agccgagcgc ggcgtgatag agccccatga
     1201 tgccggtgag gccgagccac agaagcagcg ccagccagat gggtgcgcca ccgatgggaa
     1261 gactgatgag gagccagtag gtgccgacgc cgaaggtgcc ggcgttgaag agagagccca
     1321 gccacgccga ctcgcgcggc gtggcgtcct gccacagcca catgagcgcc gcgggacaaa
     1381 gcaccgcgag cggccacagc ccgtgcggcg caaaggcggc ggcgagagcc gcgccggccg
     1441 cgagcgcgat gagggcccgc agccgtggcg aggacggccg cggcagaagc ttgagcgcca
     1501 cctaaggccc gccgcccgcg gcgcgctctt ccggcggcag cacgtcgcgc ggggccacca
     1561 cccgcagcgc ctcgatgcgg cggcggtcgg cgcgcagcac gcggaattcg aaaccgtcga
     1621 tggtcgcgct ctcgccgcgc cgcggcaggt gcccgagctg cttcatgagg aggccggcca
     1681 cggtatcgaa gccctcctcc tcggagaggc gcgccccgaa gtactcgttg aactcggcga
     1741 tgcgggtcac gccgcgcacc gtgaactagc gttccgcgtc gcggcggatg ttcagatcgt
     1801 cctcgacgtc gaactcgtcg tcgatctcgc cgacgatctg ctcgatcacg tcctcgatgg
     1861 tgacgagccc cgagaccccg ccgtactcat cgacgacgat cgccatgtgg ttgcggttgc
     1921 ggcggaactc gcgcagcagc acgttgaggc gcttggactc gggcacgaac agcgccgggc
     1981 gcatgaactc gcggatgtcg aagcgctcgc gcttctccac cgtgaggcgc agcagatcct
     2041 tggccagcag gatcccgacg atgtcgtcgc ggtcctcgtc catcaccggg aagcgcgagt
     2101 gcccggactc gaccactacc ggcaggatgc tcgcggcgcg ctcatcgcgc cggatgaaca
     2161 ccatctgcgc gcgcggcacc atgatatcgc gcaccttgag gtccgcgacc tcgagcacgc
     2221 cctcgatcat cgacagcgca tcgccgtcga ccagcccgcg gtcgccggca tcgcgcagca
     2281 ccgcgagcag ctcctggcgg tcctgcggct cgggcgcgag tccctgggtg aggcgcttca
     2341 accagcgtcc ggtcgcgttc agctccttgg tcatggcggt cgctcgaggt aggggttggc
     2401 gaagccgagg cggcgcagta cggcgatctc gcgccgctcc atccgccgcg cgtcggcctc
     2461 accctgatgg tcgtagccga tcaggtgcag cgccccgtgc acgatgaggt gcgcccagtg
     2521 cgccttcagg gccttcgcct gtgcgcgcgc ctcgccgcgc agcacgcccg ggcagatcac
     2581 caggtcaccg agcgcacgcg caccgggctg cgtgcccccc ggcatggcgg gcggcggaaa
     2641 ggacaggacg ttggtcggct gattgctcgt ctcctcaagg ttcgtaaagg aacggcctag
     2701 aacgggcata actccaagaa ccgaaaagta gtttgc
//
LOCUS       DATLKL010000009         1509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_112870, whole genome shotgun sequence.
ACCESSION   DATLKL010000009 DATLKL010000000
VERSION     DATLKL010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112870"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1509)
                     /locus_tag="VM713_00100"
     CDS             complement(<1..>1509)
                     /locus_tag="VM713_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH82692.1"
                     /translation="KINPADAPILILALTSKTLTAGQVYDAAETILSQKLSQVSGVGQ
                     VQIGGSSLPAVRVELNPNALFKYGIGLEDVRAALASANANTPKGSVDVGGRRYQIYTN
                     DQASKAAEYRNLVVAYRNGAAVRLEDVAEVLDSVENIRNLGLANGKPAVLVILYRQPG
                     ANIIKTVDRVISLIPALKAAIPTDIDMTVAMDRTTTIRASLRAVQLTLAGAIALVILV
                     VFLFLRNLRATLVPAVAVPISLLGTFGAMYLLDYSLDQLSLMALIIATGFVVDDAIVV
                     LENVTRHMEAGMPRFQAALQGAREVGFTVLSMSLSPVAVFIPILLMSGIVGRLFREFS
                     ITLSVAVLVSLVVSLTTTPMMCSRLLRRADPAEQGALFRWSERMFEGMRQLYARSLHW
                     ALDNRGVIMVLLGATVVFNVYLLFVIPKGFFPQQDTGRLVGGIQADQAISFQSMEVKL
                     RQFVAIVQADPAVETVVGFTGGGSQGGGQTNSGFIFVSLKPKSQRALSADQVI"
BASE COUNT          277 a          486 c          513 g          233 t
ORIGIN      
        1 gatgacctga tcggcggaca gcgcgcgctg cgacttcggt ttcagcgaca cgaaaatgaa
       61 gccgctgttg gtctgcccgc cgccctgtga gccgccgccg gtgaagccga cgaccgtctc
      121 cacggccgga tccgcctgga cgatggcgac gaactgccgc agcttcacct ccatcgattg
      181 gaaggagatg gcctgatcgg cctggatgcc gccgaccagc cgcccggtgt cctgctgagg
      241 aaagaaaccc ttcgggatca cgaataagag atagacattg aagaccaccg tggcgccgag
      301 cagcaccatg atcacgcccc ggttgtcgag cgcccagtgc agcgaccgcg cgtacagctg
      361 ccgcatgccc tcgaacatgc gctcgctcca gcgaaacagc gctccctgct cggccgggtc
      421 ggcgcgccgc aggaggcgcg agcacatcat cggggtagtg gtgagcgaca ctaccagcga
      481 gaccagcacc gcaaccgaca gcgtgatcga gaactcgcgg aacagccgtc cgacgatgcc
      541 gctcatgagc aggatcggga tgaacaccgc gaccggcgac aggctcatcg acaacactgt
      601 gaagccgacc tcgcgcgcac cctgcagcgc cgcctggaaa cgcggcatgc cggcctccat
      661 gtggcgcgta acgttctcca gcaccacgat cgcatcatcg acgacgaagc cggtggcgat
      721 gatgagcgcc atgagcgaca gctggtcgag ggagtagtcg agcaggtaca ttgccccgaa
      781 ggtgccgagg agcgagatcg gcaccgccac ggccggcacg agcgtcgccc gcaggttgcg
      841 caggaacagg aacaccacca ggatcacgag tgcaatcgcg cccgcgagtg tcagctgcac
      901 cgcacggagc gaggcgcgga tggtggtcgt gcggtccatg gcgaccgtca tgtcgatatc
      961 cgttggaatc gccgccttga gcgcgggaat caggctgatc acccggtcga ccgtcttgat
     1021 gatgttggcg cccggctggc ggtagaggat gacgaggacg gcaggcttgc cgttggcgag
     1081 tccgagattg cgtatgttct ccaccgagtc gaggacctcg gccacgtcct caaggcgcac
     1141 ggccgcgccg ttgcggtagg cgaccaccag gttgcggtat tcggccgcct tgctcgcctg
     1201 gtcgttggtg tagatctggt agcgccggcc gccgacgtcg accgagccct tgggcgtgtt
     1261 ggcattggcg gacgccagcg ccgcgcgcac gtcctcgagc ccgatgccgt acttgaagag
     1321 cgcgttcggg ttgagctcga cgcgcaccgc cggcagcgag ctgccgccga tctgcacctg
     1381 gccgacgccg ctcacctgcg agagtttctg cgagaggatg gtctcggcgg cgtcgtacac
     1441 ctggccggcg gtgagggtct tcgaggtgag cgccaggatc aggatgggcg catcggcggg
     1501 attgatctt
//
LOCUS       DATLKL010000010         1774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_101608, whole genome shotgun sequence.
ACCESSION   DATLKL010000010 DATLKL010000000
VERSION     DATLKL010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101608"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VM713_00105"
     CDS             complement(<1..364)
                     /locus_tag="VM713_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HVH82693.1"
                     /translation="MTRTIEIHQVDAFTREPFTGNPTGVVLNADALSDAQMLAVAREL
                     NNAETAFMLAADGADHSVRARFFTPRTEIGFVGHATVAAQYVLSRQPEAPRRQRQKSK
                     AGIVDVEVRGTGADRSIAI"
     gene            420..710
                     /locus_tag="VM713_00110"
     CDS             420..710
                     /locus_tag="VM713_00110"
                     /inference="COORDINATES: protein motif:HMM:NF019246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HVH82694.1"
                     /translation="MQYSGPARQGLTMDIGVEISLYPLKADYIPSVHEFLKDLSAHPG
                     LKVVTNSMSTQVFGGFEEVMDALRAALAASFAPLAAGSERAVFVMKVIGPLP"
     gene            794..1090
                     /locus_tag="VM713_00115"
     CDS             794..1090
                     /locus_tag="VM713_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVH82695.1"
                     /translation="MDRDGNEHEVDGKTGLKVMENLRELDYGVAAICGGMCSCATCHI
                     YVDPEWLPKLPAIMSDERELLSELAHYRENSRLSCQLEFTPALAGLRVTVAPDE"
     gene            1100..>1774
                     /locus_tag="VM713_00120"
     CDS             1100..>1774
                     /locus_tag="VM713_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVH82696.1"
                     /translation="MSTAHVQVETHGAVALVTLNRPEHSNTLNLQMAMDLLAAAMACA
                     RNAAVRAVVLTGAGRNFSFGGDLRAMPARELTLDAHIRELTTYLHAAISHFVRMDAPL
                     LAAVNGTAAGAGVGLVAMADLALCGKGSKFNLAYTSVGLTPDAGTTFLLPRTVGQKRA
                     MELLLLNRQLSAEEALSWGLVNEVVADERLLEHAVEVAERLAHGPGSAYGATKRLMAH
                     SLGAFET"
BASE COUNT          278 a          582 c          616 g          298 t
ORIGIN      
        1 ggatcgcgat gctgcggtcg gcgccggtgc cgcgcacctc gacgtcgacg atgcccgctt
       61 tggatttctg tcgctggcgg cgcggcgcct cgggttgccg cgacagcacg tattgggcag
      121 cgacggtcgc gtggcccacg aagcctatct cggtgcgcgg cgtgaaaaag cgcgcgcgta
      181 cgctgtggtc ggcgccgtcg gcggcgagca tgaaggcggt ctcggcgttg ttgagctcgc
      241 gcgccaccgc gagcatctgc gcgtcgctga gcgcgtcggc gttgaggacg acgccggtcg
      301 ggttgcccgt gaacggctcg cgggtgaagg catcgacctg atgaatctca atcgttcgcg
      361 tcatcgttcc cgtccggacc agcgcccctt gcaattctag tcctatcgca tggcttcgcg
      421 tgcaatactc cggccccgcg cggcaggggc tcaccatgga tatcggcgtc gaaataagtc
      481 tgtatccgct caaggccgac tacatcccgt ccgtgcacga gtttctcaag gacttgagcg
      541 cgcacccggg cctcaaggtc gtcactaaca gcatgagcac gcaagtgttc gggggcttcg
      601 aggaggtcat ggacgcgctg cgagccgcgc tcgcggccag cttcgcgccg ttggcggcgg
      661 gctcggagcg ggccgtgttc gtgatgaaag tgatcggacc gttgccgtag cggcctgagg
      721 ctggcggcgc cgggagcgaa tgggcacact accgacggca cagcggggaa cctgcatggc
      781 gctcttgcga gttgtggatc gcgacggaaa cgagcacgag gtggacggca agaccggcct
      841 caaggtgatg gagaacctgc gtgagctcga ctacggcgtc gcggccatct gcggcggcat
      901 gtgctcgtgc gcgacctgtc acatctacgt cgatcccgaa tggctgccga aactgcccgc
      961 catcatgtcg gacgagcgcg agctgctcag cgagctcgcg cactaccgcg agaactcccg
     1021 cctctcctgt cagcttgagt tcaccccagc gctggccggg ctgcgcgtga cagtggcgcc
     1081 ggacgagtag gtttggcaaa tgtcgacagc ccacgtccag gtcgagaccc acggcgccgt
     1141 tgcgctggtg acgctcaacc gtcccgagca ttccaacact ctcaacctgc agatggcgat
     1201 ggatctgctg gcggcggcca tggcgtgcgc ccgcaacgcg gcggtgcgcg ccgtggtact
     1261 gaccggcgcg gggcgcaatt tctccttcgg cggggatctg cgcgccatgc cggcacgcga
     1321 gctcacgctc gatgcccata ttcgtgagct caccacctac ctgcacgcgg cgatctcgca
     1381 cttcgtgcgc atggacgccc cgctgctcgc cgccgtcaac ggcacggcag ccggcgccgg
     1441 cgtcgggctg gtcgccatgg cggatctggc attgtgcggg aagggctcga agttcaacct
     1501 cgcctatacc agcgtcggac tcacgccgga tgccggcacg accttcctgc tgccgcgcac
     1561 cgtggggcaa aagcgcgcca tggagctgct gctgctcaat cggcagctga gcgccgagga
     1621 ggcgctcagc tggggactgg tgaacgaggt agtggcggac gagcggctgc tcgagcacgc
     1681 cgtcgaggtg gccgagcgcc tggcgcacgg cccgggcagc gcctacgggg ccaccaagcg
     1741 cctgatggct cactcgctcg gcgcctttga aacg
//
LOCUS       DATLKL010000011         1527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_67750, whole genome shotgun sequence.
ACCESSION   DATLKL010000011 DATLKL010000000
VERSION     DATLKL010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67750"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..>1527)
                     /gene="xrtE"
                     /locus_tag="VM713_00125"
     CDS             complement(305..>1527)
                     /gene="xrtE"
                     /locus_tag="VM713_00125"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exosortase E/protease, VPEID-CTERM system"
                     /protein_id="HVH82697.1"
                     /translation="AAALALLTALAPWTVWREVARGIGNLWWYAGGAAATSALAIAWS
                     QSFWVPTAKPTFEAVYHLLQWFIPTVHTDPASLIIDTGRFAVSIDPVCSGLEGVGLML
                     AFGSALLLVFREEYNFPRALLLLPGGVLLILALNVVRIAALVAIGHAGYGDVAVYGFH
                     SQAGWIAFNCAAGAIALVSLRSRWFSRRAAPRSSVGRTNPTAVYLMPFLAFLSAGMVS
                     RATSSGFEHLYALPAAAALVALGYSWPRLHALDWRFTWRGPAAGVGVFAAWILATQLL
                     PLSPDSRPGFGPTQAWPHALWMTTRLATTLAVAPLTEELAFRGYLLRRLRHADFESVR
                     LRSAAGWPLVVSAVAFGLYHGGMWQPWIIAGLVYGLVFIRTERIGESLAAHTTTNTLI
                     AACVLSGIAPSKFW"
BASE COUNT          306 a          492 c          497 g          232 t
ORIGIN      
        1 aggcccccgc cggatcgatc tcgggcgcag agacaaccct cttgggtcga tcgtggtcac
       61 cgcccccatc ataggggccg tctccatcgc cattcgccac ggcggcagtt gcgagcatag
      121 acaggatgag cacgccagct gtttttctca tttttaagct tccctgcggt ccccggtggg
      181 atggtgaagt gcttgtgtgg tcggcaggat aacaaccttg tcgccagagg gctacggggc
      241 ataactacca cgccacgcca ttatctctgg agcggagccc attccagcgt ttctgtgacg
      301 cggttcacca aaacttcgaa ggcgcgatgc cacttagtac gcatgctgca atcagcgtgt
      361 tcgtcgtcgt gtgggccgca agcgattcac cgattcgctc cgtgcgaata aaaaccaagc
      421 catagacgag gcccgcgatg atccacggct gccacattcc gccgtggtag agtccaaacg
      481 ccaccgcgga aacgacgagt ggccacccgg cggcgctgcg cagccgcacg gactcgaaat
      541 cggcgtgtcg aaggcgacgc agcagatagc cgcggaaagc gagttcctct gtgagcggcg
      601 ccacggcgag tgtcgtcgcg aggcgcgtcg tcatccacaa tgcgtgcggc cacgcctgcg
      661 ttgggccgaa cccggggcgg ctgtcaggag acagggggag gagctgcgta gcgagtatcc
      721 aagccgcgaa gactcccaca cccgccgcgg gtccgcgcca agtgaagcgc cagtcgagtg
      781 cgtgcaggcg cggccaggag tagccgagcg ccacgagtgc cgcggccgcc ggcagcgcgt
      841 acaggtgctc gaagccgctc gaggtcgcgc ggctcaccat acccgccgag agaaacgcca
      901 ggaacggcat gagatacacg gcggtcgggt tggtacggcc aacgctcgag cgcggcgcgg
      961 cgcgacggct gaaccaacgg ctgcgcagac tcaccagtgc gatcgcgccg gccgcgcaat
     1021 tgaaggcgat ccagccggcc tgcgagtgga agccgtacac ggcgacatct ccatagccag
     1081 cgtggccgat cgccaccagc gcggctattc gcaccacgtt gagagcgagt atgagcagca
     1141 cgccgccggg cagcagcagc aaagcgcgag ggaaattgta ttcctcgcgg aacacgagca
     1201 gcagcgcgct gccgaaggcc agcatcagac ccacgccctc caatcccgag cacaccgggt
     1261 cgatgctcac tgcaaagcgc ccggtgtcga tgatcaggct tgcgggatcg gtgtgcacgg
     1321 tggggatgaa ccattgcaac aggtggtaga cggcttcgaa ggtcggcttg gcagtcggta
     1381 cccaaaacga ctggctccac gcaatcgcta gtgcggaggt cgcggctgcc cctccggcgt
     1441 accaccagag attgcctatt cctcgagcga cttcgcgcca taccgtccac ggtgccaatg
     1501 ccgttaagag cgcgagcgcg gcagccg
//
LOCUS       DATLKL010000012         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_169271, whole genome shotgun sequence.
ACCESSION   DATLKL010000012 DATLKL010000000
VERSION     DATLKL010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169271"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /gene="panC"
                     /locus_tag="VM713_00130"
     CDS             complement(<1..737)
                     /gene="panC"
                     /locus_tag="VM713_00130"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HVH82698.1"
                     /translation="METVTTISAVRERVRVWRAAGARVVFVPTMGNLHAGHVSLIETA
                     RRHGERFVASIFVNPMQFGPNEDFAHYPRTPREDERMLTRAGCDLMFMPDVAEIYPNG
                     SERATRVEVPGLSRILEGEFRPGHMEGVSTVVAKLFHIVEPDVAVFGEKDFQQLAVIR
                     RMVAELCMRVEIVGAPTLRDTDGLALSSRNQYLTPAERRLAPVIYATLAAAAARVRAG
                     DQQFASIERSGFQALEGAGLRPDYFSVR"
     gene            complement(776..1600)
                     /gene="panB"
                     /locus_tag="VM713_00135"
     CDS             complement(776..1600)
                     /gene="panB"
                     /locus_tag="VM713_00135"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HVH82699.1"
                     /translation="MYTHLQRDATRPPVTLATLARMKADGEKIASLTCYDASFAVLLD
                     AADVDVVLVGDSLGMVIQGHSTTVPVTMDQMVYHCACVARGLHRPFLIGDLPFMSYPS
                     REVALTNSVRMMQEGGAQMVKLESGGKQTDVVEFLASHDIAVCAHVGLKPQSVHKTGG
                     FRVQGRERETATRLLEEARRLESAGADIVLLECIPASLGKAIAAELHVPVIGIGAGPD
                     TDGQILVTYDMLDITTGRKPRFVRNFMEGAGDNLEALKRYVRAVKQREYPAPEHCF"
     gene            complement(1663..>2036)
                     /locus_tag="VM713_00140"
     CDS             complement(1663..>2036)
                     /locus_tag="VM713_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HVH82700.1"
                     /translation="LFARVTLDDAEYALYEQLYALLDVQVPKPDLVVYLQAPVDVLLE
                     RVARRGVAYEQYIDRGYLERLNEAYARFFHEFDSAPLLIVNAASIDPVSNQHDYDELL
                     AAIKRMSRGRLYYNPLRSRAI"
BASE COUNT          322 a          665 c          722 g          327 t
ORIGIN      
        1 cgcacggaga agtagtcggg gcgcaggccc gcgccctcca gcgcctggaa gccggagcgc
       61 tcgatgctgg caaactgctg gtcgccggcc cgcacccggg cggctgccgc tgccagcgtc
      121 gcgtagatga ccggcgccag cctgcgctcg gcgggcgtga ggtactggtt gcgcgagctc
      181 agcgcgagcc cgtcggtatc gcgcagggtc ggggcgccga cgatctcgac ccgcatgcag
      241 agctcggcga ccatgcggcg gatcaccgcg agctgctgaa aatccttctc gccgaacacc
      301 gccacgtcgg gctcgacgat gtgaaagagc ttcgccacca cggtgctgac gccttccatg
      361 tggccggggc ggaactcgcc ctcgaggatg cgcgacagcc ccggcacctc gacccgcgtc
      421 gcgcgctcgg agccgttggg atagatctcg gccacgtccg gcatgaacat gaggtcgcag
      481 ccggcccggg tgagcatgcg ctcgtcctcg cgcggcgtgc gcgggtagtg ggcgaagtcc
      541 tcgttgggac cgaactgcat cggattcacg aagatgctgg cgacgaagcg ctcgccgtgg
      601 cggcgtgcgg tctcgatgag gctcacgtgt ccggcgtgca ggttccccat ggtcggcacg
      661 aatacgaccc gcgcgccggc ggcccgccag acgcgcaccc gttcgcggac cgcgctgatg
      721 gtggtgacgg tctccatggg tcgcggtcga cgcaggcgcg gctcttagcg ccgcttcaga
      781 agcagtgctc gggagcggga tactcgcgct gcttgacggc gcgcacgtag cgcttgaggg
      841 cctcgagatt gtcgcccgcg ccttccatga agttgcgcac gaagcgcggc ttgcgcccgg
      901 tcgtgatgtc gagcatgtcg taggtcacca ggatctgccc gtcggtgtcg ggtccggcgc
      961 cgatgccgat caccggcacg tgcagctccg cagcgatggc tttgccgagc gaggcaggta
     1021 tgcactcgag cagcacgatg tcggcgccgg cggactccaa ccgtcgcgcc tcctcgagca
     1081 ggcgggtggc ggtctcgcgc tcgcgcccct gtacgcggaa accgccggtc ttgtgtaccg
     1141 actgcggctt gagccccacg tgggcgcaca cggcgatgtc gtggctcgcg aggaactcga
     1201 cgacgtcggt ctgcttgccg ccgctctcga gcttcaccat ctgtgcgccg ccctcctgca
     1261 tcatgcggac cgagttcgtg agcgccacct cgcgcgaggg gtagctcatg aacggcaggt
     1321 cgccgatcag gaacgggcgg tggagccccc gcgccacgca ggcgcagtgg tacaccatct
     1381 gatccatggt gaccgggacc gtcgtgctgt gcccctggat caccatgccg agcgagtctc
     1441 ccaccagcac tacgtcgaca tctgcggcgt cgagcagcac ggcaaagctc gcgtcataac
     1501 aggtgaggct ggcgatcttc tcgccgtcgg ccttcatgcg ggcaagcgtc gcgagcgtga
     1561 ccggcgggcg ggtggcgtcg cgttgcagat gggtgtacat gggcgtcgag agtgtaacgg
     1621 cacgctgcgc agccggcaaa aaccacgctt tttaccggcg gactatatcg cgcggctgcg
     1681 caacggattg tagtagaggc gcccgcggct catgcgcttg atggccgcca gcagctcgtc
     1741 gtaatcatgc tggttgctga cggggtcgat ggatgcggcg ttgacgatga ggaggggcgc
     1801 cgagtcgaac tcgtggaaaa agcgcgcata agcctcgttg agccgctcga gatagccgcg
     1861 atcgatgtac tgctcgtaag cgacgccacg gcgggcgacg cgctcgagga gcacgtcgac
     1921 gggggcctgc agatacacca ccaggtcggg cttcggcacc tggacgtcga gcagcgcata
     1981 gagctgctcg tagagcgcgt actcggcgtc atcgagcgtc acgcgggcga acagcc
//
LOCUS       DATLKL010000013         1617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_157972, whole genome shotgun sequence.
ACCESSION   DATLKL010000013 DATLKL010000000
VERSION     DATLKL010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157972"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VM713_00145"
     CDS             <1..208
                     /locus_tag="VM713_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HVH82701.1"
                     /translation="TPVEITAPYAYFRLRDEGYTPDDLVRWAGTIRASTAQCSDVFVY
                     FKHEEAGKGPQFAKALLEALAAGA"
     gene            306..746
                     /locus_tag="VM713_00150"
     CDS             306..746
                     /locus_tag="VM713_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HVH82702.1"
                     /translation="MKEFGPFAVAIAFWIFVAVAAVTGIWADYKKRQAALEPLRTAIE
                     RGQQLDPALVERLMAPERDAGINPQYLRVGGIITLSAGVGVVILAFLLNQVVPLSFYP
                     VLGGGIVTVCVGAGLMIAARSIEQERKPAAAAGPGGGLRETRGT"
     gene            754..1332
                     /locus_tag="VM713_00155"
     CDS             754..1332
                     /locus_tag="VM713_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVH82703.1"
                     /translation="MNPVSAALGEAAEAVVVALAMGGDRSAFCELVWRRQSWLRNLLR
                     RLCRDPALADDLAQQVFLKAWRSLSQLKAPGAFGGWLRRLAVNTWLAEVRARTPADLA
                     QQPEELSAATAFMPAAAEQLDLDRALAKLTHTERLCVVLAYSEGMSHGEISAVTSLPL
                     GTVKSHVRRGAQRLRVLLDAYQSPGKEHAHAG"
     gene            1322..1594
                     /locus_tag="VM713_00160"
     CDS             1322..1594
                     /locus_tag="VM713_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82704.1"
                     /translation="MLDDRDALLVREFAKSQRPLADAQFVAQVTERLHVYSVRRLLGV
                     VLSGVIRAVGAGLSFGVVAPLRMRHAGLAALAALGIVLWNLVSRSL"
BASE COUNT          232 a          555 c          553 g          277 t
ORIGIN      
        1 caccccggtc gagattaccg cgccgtacgc ctacttccgg ctgcgcgacg agggctatac
       61 gcccgatgat ctcgtgcgct gggctgggac catccgcgcg agcacggcgc aatgcagcga
      121 tgtgttcgtg tatttcaagc acgaagaggc cggcaagggt ccgcagttcg cgaaagcgct
      181 gctcgaggcg ctggcggcgg gcgcttaagc ggccgaaccg ggaatttccg cgctgcccct
      241 gcatccaaac gcgcaccggc ccgcatcttt acgggtacct catctcatta ccggggatta
      301 agcccatgaa ggaattcgga ccgttcgccg ttgccatcgc tttctggatc ttcgtcgccg
      361 tcgccgccgt caccggcatc tgggccgact acaagaagcg ccaggcggcg ctcgaaccgc
      421 tgcgcacagc gatcgagcgc ggccagcagc tcgacccggc tctggtcgag cgtctcatgg
      481 cgcccgagcg cgacgccggc atcaacccac agtacctgcg cgtcgggggc atcatcacgc
      541 tgtcggccgg tgtgggcgtc gtgatcctgg cattcctgct caatcaggtc gtgccgctct
      601 ccttctatcc cgtgctcggc ggaggcatcg tgacggtctg cgtgggggcc gggctcatga
      661 tcgcggcgcg ctccatcgag caggagcgca aaccggcggc ggccgccggc ccgggcggcg
      721 ggttgcggga aacgcgcgga acctgagcgt agcgtgaatc ccgtgagcgc cgcgctcggc
      781 gaggctgcgg aggccgtcgt ggtcgccctc gcgatgggcg gcgaccgcag tgccttctgc
      841 gagctcgtat ggcgccgcca gagctggttg cggaatctcc tgcggcgcct atgccgcgat
      901 cccgcgctcg ccgacgacct cgcacagcag gtgttcctca aggcgtggcg ctcgctgtct
      961 cagcttaaag ccccaggcgc cttcggcggc tggctgcggc gcctcgccgt aaacacgtgg
     1021 cttgccgagg tgcgggcacg aaccccagcc gacctggcgc agcagccgga ggaattatcg
     1081 gccgcgaccg cgttcatgcc cgccgcggcc gagcagctcg acctcgatcg ggcgctcgcg
     1141 aaactgactc ataccgagcg gctatgcgtc gtgctcgcct acagtgaggg gatgtcgcat
     1201 ggcgagatca gcgccgtgac ctcgctgcca ctcgggaccg tcaagtccca cgtccggcgc
     1261 ggcgcgcagc gactgcgggt gctgctcgat gcctatcaat cgcccgggaa ggaacatgcc
     1321 catgctggat gatcgcgatg cactgttggt gcgcgagttt gcgaagagtc aacggccgtt
     1381 ggccgatgcg cagttcgtgg cacaggtgac ggagcggctg catgtctact ccgtgcggcg
     1441 gttgctcggg gtggtcctga gcggcgttat ccgcgccgtc ggcgccggcc tctccttcgg
     1501 cgtcgtcgcg ccgctccgga tgcgccacgc agggctcgcg gcgctggcgg cgctcggcat
     1561 cgtgctctgg aacctcgtca gccgttccct ctagaacccg ccggcgcgtt tcccggc
//
LOCUS       DATLKL010000014         1598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_180547, whole genome shotgun sequence.
ACCESSION   DATLKL010000014 DATLKL010000000
VERSION     DATLKL010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180547"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="VM713_00165"
     CDS             complement(<1..1497)
                     /locus_tag="VM713_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HVH82705.1"
                     /translation="MLPELGHFALILALLLAALQAFFGIAGPMFHRERWVAAVTPAVA
                     GQAVMVATAVGCLIYSFVNDDFSVAYVAENSNSALPLLYRVAALWGAHEGSLLLWIML
                     LALWTVAVAMYANRLPRLFAARVLGVLGVISFGFLLFTLATSNPFLRLDPPPLDGRDL
                     NPILQDPGLAGHPPVLYTGYVGFAVAFAFACGAMLEGRMDQSWARWTRPWVTAAWAFL
                     TCGIAMGSFWAYYELGWGGFWFWDPVECASFMPWLVGTALIHSLAVTDKRGLFKSWTL
                     LLSVLAFSLSLLGTFLVRSGVLVSVHSFAADPSRGVFILSFLIVMIGGALSLYARRAP
                     LLKSPAGFEFGARESFLLFNNVLLVMAAGTVFAGTLAPLISGSLKLGTVSVGPQFFNP
                     TFMLAMLPLLALLSIGIHASWKRGRLGTQQRTLLYTFVAAAVLGCGVVYGIYSHGHLL
                     TPVGATLGIWIILSSLVDPVDRVRRRLSLPRAVVGMTIAHIGLATAVLA"
BASE COUNT          326 a          553 c          543 g          176 t
ORIGIN      
        1 ggcgagcacc gcggtcgcga gcccgatgtg cgcgatcgtc atgccgacca ccgcgcgcgg
       61 cagcgacagt cggcggcgca cgcgatccac cgggtcgacg agcgacgaga ggatgatcca
      121 tatcccgagc gtcgcgccca ccggcgtcag caggtgcccg tgggaataga tgccgtagac
      181 caccccgcag ccgagcaccg cagcggccac gaacgtatag aggagcgtgc gctgctgcgt
      241 cccgagccgc ccccgcttcc agctcgcgtg gatgccgatc gacagcagcg cgagcagcgg
      301 cagcatcgcc agcatgaagg tcgggttgaa gaattgcggg ccgaccgaga cggtgccgag
      361 cttgagcgag cccgaaatca gcggtgccag cgtaccggcg aacaccgtac cggcggccat
      421 caccagcagc acgttgttga acagcaggaa cgactcgcgc gccccgaatt cgaagccggc
      481 cggggatttc agcagcggcg cacgccgggc atagaggctg agcgccccgc cgatcattac
      541 gatgaggaac gagaggatga acacgccgcg gctcggatcg gcggcgaagg aatgcaccga
      601 caccagcact ccggagcgca ccaggaaggt gccgagcagg ctcaacgaga acgccagtac
      661 cgagagcagc agcgtccagc tcttgaagag cccgcgcttg tcggtgaccg cgagcgaatg
      721 gatgagcgcc gtacccacca gccacggcat gaacgaggcg cactccaccg gatcccagaa
      781 ccagaagcca ccccagccga gctcgtaata cgcccagaag ctccccatgg cgatcccgca
      841 ggtgaggaac gcccaggccg ccgtgaccca gggccgcgtc cagcgcgccc aactctggtc
      901 catgcggcct tcgagcatgg cgccgcaggc gaaggcgaac gccaccgcga agccgacgta
      961 gcctgtgtag agcactggcg gatgaccggc gagtcccggg tcctgcagga tcgggttgag
     1021 gtcgcgcccg tcgagcgggg gcgggtcgag ccgcaggaag ggatttgagg tcgcgagcgt
     1081 gaacaacagg aagccgaagc tgatcacccc gagcaccccg agcacccgcg ccgcaaacaa
     1141 ccgtggcagc cggttcgcgt acatcgcgac cgcgacggtc cacagcgcga gcagcatgat
     1201 ccacaggagc agcgatccct cgtgcgcgcc ccacagggcc gcgacccggt acaagagcgg
     1261 cagcgcggag ttggaattct ccgcgacgta ggcgaccgag aagtcatcgt tcacgaacga
     1321 gtagatgaga caaccgaccg cggtcgcgac catgactgcc tggcccgcga ccgccggtgt
     1381 caccgccgcg acccaccgct cgcggtgaaa catcgggccg gcgattccga agaatgcctg
     1441 cagcgccgcg agcagcagcg cgagaatcaa cgcaaagtga ccgagctccg gcagcatcct
     1501 cagctccctg tgttggcggg cgaccccgcg gtcgccgtcg tgggcggcgc cgtacgcggc
     1561 gggcccgcgg cgggcgccgg caagccctcg cgcgactc
//
LOCUS       DATLKL010000015         1866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_101653, whole genome shotgun sequence.
ACCESSION   DATLKL010000015 DATLKL010000000
VERSION     DATLKL010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101653"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VM713_00170"
     CDS             complement(<1..1097)
                     /locus_tag="VM713_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVH82706.1"
                     /translation="MTPLSYGNVLEMIGRTPLMAVRRIDTGPCQLFLKLENQNPGGSI
                     KDRIGLYLIQAAERAGQIRAGSVLIEATAGNTGLGLALVAAQKGYRLMLAIPDKMSQE
                     KIFHLKAMGAEVVMTRSDVSKGHPEYYRDVAERLAGEVPNAYFVNQFGNPVNAQAHEE
                     TTAPEIWEQMGGRLDAVVCGCGTGGTLAGLSHFFARAAPRVAMVLADPVGSGLASFAS
                     TGKLPEKMSPWLVEGIGGDTVPSVADFSRVTATYSIPDPEAFATCRELLKQEGIIAGT
                     STGTLLAAALRYCRAQTAPKRVVTFVCDSGNKYLSKVYNDYWMLDQGFLKRESHGDLR
                     DLIVRRHKDRAAVTVNSTETVLAAYRRMKLY"
     gene            complement(1181..>1866)
                     /locus_tag="VM713_00175"
     CDS             complement(1181..>1866)
                     /locus_tag="VM713_00175"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HVH82707.1"
                     /translation="VETRLGIYGGAYGSRLIDALESSYPALAALLGEEDFRILGARYV
                     RAHDSPYFSIRNYGDALAEFLSAHADYAEVPLLAELARWEWMLCAVFDAADAEPLTAA
                     ALARLPPADWPQLRLRFHPSVRRLGLRWNAPQLWQALTDGAERPELRVVAEPIEWLAW
                     RQDLTTYFRSLAAGEAAALDAARNGRSFGELCELLAATHGPERAPAEAATLLAGWLGG
                     GLLIAAVPA"
BASE COUNT          284 a          630 c          655 g          297 t
ORIGIN      
        1 tcgtagagct tcatgcggcg gtaggccgcc agcaccgttt cggtggaatt caccgtgacc
       61 gcggcgcgat ccttgtggcg acgcacgatg agatcgcgca gatcgccgtg gctctcgcgc
      121 ttgaggaagc cctgatcgag catccagtaa tcgttgtaga ccttggagag gtacttgttg
      181 ccgctgtcgc acacgaaggt cacgacgcgc ttgggcgcgg tctgtgcacg gcagtagcgc
      241 agcgccgccg cgagcagcgt gccggtcgag gtgcccgcga tgatgccttc ctgcttcagc
      301 agctcgcggc aggtggcgaa ggcctcgggg tcgggaatgc tgtaagtcgc ggtcacgcgc
      361 gagaagtctg cgaccgacgg cacggtgtcg ccgccgatgc cttcgaccag ccacggcgac
      421 atcttctccg ggagcttgcc ggtgctggcg aagctcgcca gccccgagcc caccggatcg
      481 gcgagcacca tcgcgacgcg cggcgcggcg cgggcgaaga agtgcgacag tccggccagc
      541 gttccgccgg tgccgcagcc gcacacgacg gcatcgagcc tgccgcccat ctgctcccag
      601 atctcgggcg cggtggtctc ctcatgtgcc tgagcgttga ctggattgcc gaactggttg
      661 acgaaataag cattgggtac ctcgccggcg aggcgctcgg cgacgtcgcg gtagtactcc
      721 ggatggccct tgctgacatc ggagcgggtc atcaccactt ccgctcccat tgccttgagg
      781 tgaaagatct tctcctggct catcttgtcc gggatcgcga gcatcaggcg gtagcccttc
      841 tgcgctgcca ccagcgcgag ccccaagccc gtgttgcccg cagtcgcctc gatgagcacg
      901 ctccccgcgc ggatctggcc ggcgcgttcg gcggcctgga tgaggtagag accaatgcga
      961 tccttgatcg agccgccggg gttctggttc tcgagcttga ggaacagctg acacgggccg
     1021 gtgtcgatgc ggcgcactgc catgagcggc gtgcggccga tcatctccag cacattgccg
     1081 tagctaagcg gtgtcatgtc cgatcctgca gcgcttaccg tgcgccggcc tgactctcac
     1141 agccgccgcg ggtcgagtgg gtggtgcagg gattgtacgg tcaggcgggg acggcggcga
     1201 taaggagtcc gccgccgagc catccggcga ggagcgtggc tgcctcggcc ggagcccgct
     1261 cggggccgtg cgtggcggcg agcagctcgc acagctcgcc gaagctccgg ccgttgcgcg
     1321 ccgcatcgag cgccgcggcc tcgcccgcgg cgagcgagcg gaagtaggtc gtgaggtcct
     1381 gccgccaggc gagccactcg atgggctccg ccacgacgcg cagctccggc cgctcggcgc
     1441 cgtcagtcag cgcctgccac agctgcgggg cgttccacct gagccccagc cgccgtacgc
     1501 tcgggtgaaa cctgaggcgc agctgcggcc agtccgcggg cgggaggcgc gcgagcgccg
     1561 cggcggtgag cggctcggca tcggcagcat cgaacaccgc gcacagcatc cattcccagc
     1621 gtgccagctc cgcgagcagc ggcacctcgg cgtagtcagc gtgcgcactg aggaactccg
     1681 cgagcgcgtc gccgtaattc ctgatggaga agtacggcga gtcgtgcgcg cgcacgtacc
     1741 tggcgcccag gatgcggaaa tcctcctcgc cgaggagcgc ggcgagcgcc ggatagctgc
     1801 tctcgagcgc gtcgatcagg cggctgccat aggcgccgcc atagatcccg agccgcgtct
     1861 cgaccg
//
LOCUS       DATLKL010000016         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_22696, whole genome shotgun sequence.
ACCESSION   DATLKL010000016 DATLKL010000000
VERSION     DATLKL010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22696"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /gene="lysA"
                     /locus_tag="VM713_00180"
     CDS             <1..1310
                     /gene="lysA"
                     /locus_tag="VM713_00180"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HVH82708.1"
                     /translation="YLVSQAANDLNFTFVVDENQGDRLVEQLHELLIRPVPGDQVLGP
                     TWQQLFAAPPERAARVPPWWRTRRAQLLEALGEREAAYVYDLATVAAAARALRELKSL
                     DRVHYSMKANPHPEILRTLRGAGVEFECVSRGEVEHLLATCPDLPPERVLYTPNFAPR
                     MEYAWALERGVRVTVDNTSVLTAWPALFRGREIFARLDTGVGRGHHLHVRTAGTRSKF
                     GVPIAELAAFERAARAAGARITGLEAHIGSGIFDVTSWEHTARLLAAAAHSVPEVRVI
                     DVGGGLGVPERADQPGVDLARLDTLLKAVRAEHPGLEVWLEPGRYLVAAAGVLLARVT
                     QLKQKGGVRFVGVATGMNSLIRPALYGAYHEIANLTRLDEPATELVSIVGPICESADV
                     LGHDRLLPVTHAGDVLLIANAGAYGHVMSSHYNLRSPAAELIL"
     gene            1364..>2043
                     /locus_tag="VM713_00185"
     CDS             1364..>2043
                     /locus_tag="VM713_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HVH82709.1"
                     /translation="MLYLDLLVTRKFEGRRWTLPAQVYAAPLELYAGLALPQSDVSHE
                     LERLHYRRVGQLDRPGTYRLSGARIDVALRAAHFADENRDAQMLSILTGPAGIESLKD
                     AAGHDVPVMRLEPLLIGSIFPSHGEDRIVLTPREVPPLLPAALKAVEDRRFDSHRGVD
                     PQAILRALWVDARAGQIEQGGSTLTQQLVRSYFLSSRQTLGRKLREAIMAVALDAHFT
                     KDDLMNAY"
BASE COUNT          282 a          732 c          715 g          314 t
ORIGIN      
        1 tctacctggt gagccaggcg gccaacgatc tgaacttcac cttcgtggtc gatgagaacc
       61 agggcgatcg cctcgtcgag cagctgcacg agctgctcat ccgcccggtg cccggcgacc
      121 aggtcctcgg ccccacctgg cagcagctgt tcgcggcgcc gcccgagagg gccgcgcgcg
      181 tgccgccctg gtggcgtacc cggcgcgcgc agctgctcga ggcgctcggc gagcgcgagg
      241 ccgcttacgt ctacgacctc gcgacggtgg cagccgcagc gcgtgcgctc cgggagctca
      301 aatccctcga ccgcgtgcac tattcgatga aggccaaccc gcatccggag atcctgcgca
      361 cgctgcgcgg ggcgggggtc gagttcgagt gcgtctcgcg cggcgaggtc gagcacctgc
      421 tcgccacctg tcccgacctc ccgccggagc gcgtcctcta tacgccgaat ttcgcgccgc
      481 ggatggagta cgcctgggcg ctcgagcggg gcgtgcgggt cacggtcgac aatacttccg
      541 tgctcacggc ctggccggcg ctgttccgcg gccgcgagat attcgcgcgc ctcgacaccg
      601 gcgtgggccg cggccaccac ctgcacgtgc gcaccgccgg tacgcgctcg aagttcggcg
      661 tgccaatcgc ggagctcgcg gcgtttgagc gcgcggcgcg cgcggcgggc gcgcgcatca
      721 ccggcctcga ggcccatatc ggcagcggca tcttcgatgt gaccagctgg gagcacacgg
      781 cgcggcttct ggccgccgcc gcccacagcg ttcccgaggt gcgcgtgatc gacgtgggtg
      841 gcgggctggg cgtcccggag cgggcggacc agccgggcgt cgatctcgcc cggctcgata
      901 cgctcctcaa ggcggtgcgg gccgagcatc cgggcctcga ggtctggctc gagcccggac
      961 gctacctggt ggcagccgcc ggcgtgctgc tcgcgcgcgt gacccagctc aagcaaaagg
     1021 gcggcgtgcg cttcgtcggg gtcgccaccg gcatgaactc cctgatccgt ccggcactct
     1081 atggcgcata ccatgagatc gccaacctca cccgcctcga tgagccggcg accgagctcg
     1141 tgagcatcgt cggtcccatc tgcgagagcg ccgacgtgct cggccacgac cgcctgctgc
     1201 cggtgacgca cgcgggcgat gtgctgctca tcgcgaacgc gggcgcctat gggcacgtca
     1261 tgagctcaca ttacaacctg cgcagccctg ccgcggagct gatcctctga ggcgctgggc
     1321 tattcgcctg ctcggagcgg ccgcgatcgt cggcgccgct tacgtgctct acctcgatct
     1381 gctggtgacc cgcaagttcg aggggcggcg ctggacgctg ccggcccagg tctacgccgc
     1441 gccgctcgag ctgtatgcgg gcctggcgct gccgcaatct gacgtgagtc atgagctcga
     1501 gcggctgcat taccgcaggg tggggcagct ggaccgcccg ggcacgtacc gcctgagcgg
     1561 cgcccgcatc gacgtggcgc tgcgggcggc gcacttcgcg gacgagaatc gcgacgcgca
     1621 gatgctcagc atcctcaccg ggcctgccgg gatcgagagc ctcaaggacg ccgccgggca
     1681 cgacgtgccg gtgatgcggc tcgagccgct gctcatcggc agtatctttc cgagccacgg
     1741 cgaggatcgc atcgtgctca ccccgcgcga ggtgccgccg ctgctgccgg ctgcgctcaa
     1801 ggcggtcgag gaccgccgct tcgactcgca ccgcggcgtc gatccgcagg cgattctgcg
     1861 cgcgctgtgg gtcgatgcgc gcgccgggca gatcgagcag ggcggcagca cgctgactca
     1921 gcagctggtg cgcagctatt tcctctcgtc gcgccagacg ctcgggcgca agctccgcga
     1981 ggcgatcatg gcggtcgcgc tcgacgcgca cttcaccaag gacgatctca tgaacgccta
     2041 tat
//
LOCUS       DATLKL010000017         1650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_67825, whole genome shotgun sequence.
ACCESSION   DATLKL010000017 DATLKL010000000
VERSION     DATLKL010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67825"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VM713_00190"
     CDS             complement(<1..468)
                     /locus_tag="VM713_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82710.1"
                     /translation="MAISGELDLKNGWAQLANARYAPESVSSGAGQSSRRHRDAETLT
                     VLTTLAEVRAAINEIAAAAQRLISIYTPDLEPDLYDQTPFLEVVKHFVLTRSFSKVRV
                     LLAEPTRVMRDSNRFVAMGRRLSSCIDIRYVTAQSSQRASAYLIADDRAIVYRL"
     gene            complement(675..>1650)
                     /gene="purB"
                     /locus_tag="VM713_00195"
     CDS             complement(675..>1650)
                     /gene="purB"
                     /locus_tag="VM713_00195"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HVH82711.1"
                     /translation="YARLLQSARQTLRDILKARIDELVALAHRYAESAMLARTHGQAA
                     SPTTLGKELANFAARLMRADSRWKAVTILGKWNGAVGNFNAHAATLPAVDWPAVARAF
                     VESLGLEYNAYTTQIEPHDWIAEYCDAIAAVNVVLIDLCRDLWGYISLGYLRQRMVAD
                     EVGSSTMPHKVNPIDFENAEGNLGLANALLRHFAEKLPVSRWQRDLTDSTVLRNLGVA
                     LGHTLIAWSALGRGLARIEADAARMTSDLADSWEVLAEAVQTALRAAGVPDGYERLKK
                     FTRGRAVDARSLGEFIDGLPLPAADKARLRALKPADYVGLAATLARGI"
BASE COUNT          264 a          516 c          589 g          281 t
ORIGIN      
        1 caggcgatag acgatggcgc gatcgtcggc gatcaggtag gcggaagcgc gttgcgacga
       61 ttgcgcggtc acgtagcgga tgtcgatgca gctcgagaga cggcggccca tggcgacgaa
      121 gcggttgctg tcgcgcatca cccgggtcgg ctcggcgagc agtacgcgca ccttggagaa
      181 gctgcgcgtc agcacgaagt gtttgacgac ctcgaggaac ggggtttggt cgtagagatc
      241 gggctcgagg tccggtgtat agatagagat gagtcgctgc gcggcggcgg cgatctcgtt
      301 gatcgcggcg cgtacctcgg ccagcgtcgt gaggacggtg agggtctcgg cgtcccggtg
      361 gcgccgcgag ctctgaccgg cgccggagct taccgactcc ggtgcatagc gcgcgttggc
      421 gagctgtgcc cagccgttct tgagatcgag ttccccagaa attgccacga gtggcaccca
      481 ccccccgccc gcacgttgtg caggctacgg gcgatcttag tgatcgcccg gtccttttca
      541 ttgtcgaacc ggtcacggtt tgacgggccg gcacggatac agtaagtcaa aaaagcggcg
      601 cagttcgcat ttttaacaac ccggttatgt cgtcttcctg cgacaggtgg cgtgtacagc
      661 cccgctccgg gggctcagat gccgcgtgcc agcgtggcgg cgaggcccac gtaatcggcg
      721 ggcttgagcg cgcgcagccg cgccttgtcc gctgcgggca acggcaggcc gtcgatgaac
      781 tccccgagcg agcgcgcatc gacggcgcgg ccgcgggtga atttcttgag ccgctcatag
      841 ccgtccggga cacccgcggc gcgcagcgca gtctgcacgg cctccgcgag cacctcccag
      901 gagtcggcca ggtcgctggt catgcgcgcg gcgtcggcct cgatgcgcgc gagtccgcgc
      961 ccgagcgcgc tccaggcaat gagcgtgtgt ccgagcgcca cgcccaggtt tcgcagcacg
     1021 gtcgagtcgg tgaggtcgcg ctgccagcgc gacaccggca gcttctcagc gaagtggcgc
     1081 agcaacgcgt tggcgagccc gaggttgccc tcggcattct cgaaatcgat cggattcacc
     1141 ttgtgcggca tggtggagga gccgacctcg tccgccacca tgcgctggcg caggtagccg
     1201 agcgagatgt agccccacag gtcgcggcat agatcgatca gcacgacgtt gacggcggcg
     1261 atggcgtcgc agtactcggc aatccagtcg tggggctcga tctgggtggt gtaggcgttg
     1321 tactcgagcc cgagcgattc cacgaaggcg cgggcaaccg ccggccagtc caccgcgggc
     1381 agcgtcgcgg cgtgcgcgtt gaaattgccg accgcgccat tccactttcc gagaatagtg
     1441 acggccttcc agcgagagtc tgcgcgcatg aggcgcgcgg cgaagttggc gagctctttg
     1501 ccgagggtcg tcggactcgc cgcttgtccg tgggtgcgag cgagcatggc gctctccgca
     1561 taacggtgcg cgagcgccac cagctcatcg atgcgcgcct tgaggatgtc gcgcagggtc
     1621 tggcgcgccg actgcagcag tcgcgcgtag
//
LOCUS       DATLKL010000018         3424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_124203, whole genome shotgun sequence.
ACCESSION   DATLKL010000018 DATLKL010000000
VERSION     DATLKL010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124203"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VM713_00200"
     CDS             <1..491
                     /locus_tag="VM713_00200"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HVH82712.1"
                     /translation="RLGDSICVQGCCLTVTERAGPSFAADLSRETLSLTTLGELAVGA
                     AVNLEPALRAGDALGGHLVSGHVDGIARVTSVESDARSRRLAIAVPRELARYIAPKGS
                     VTVDGVSLTVNEVAGESFGVNIIPHTQTATTLGAARVGTPVNIEVDQVARYLARLLSV
                     PD"
     gene            567..1010
                     /locus_tag="VM713_00205"
     CDS             567..1010
                     /locus_tag="VM713_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82713.1"
                     /translation="MRAGSPFRLTARQRTTLLALVLLPALALRALVPVGFMPVVDDTG
                     IHIGFCPGEAQPPGALAGALAAGHGAHAAHHHDHGGANHGSPAPVSHPPCLFALSASP
                     AFTPAAPTLAPPPPAAAALAADSSSRVSVPAIVRAQSSRGPPFLA"
     gene            1128..3338
                     /locus_tag="VM713_00210"
     CDS             1128..3338
                     /locus_tag="VM713_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVH82714.1"
                     /translation="MKIHLASAFIAILVLLVLPSVVRAADLGDVHGVVHDAQHRPVAA
                     AAVELKAATSDWSQTTRSSGGGEFSFPGVPLGNYLLTVSRSGFVTAVMPLTVLPGAAP
                     STHVQLGAGESLETVTVSATVPASADTFTSSTLVDRGDIQSTPGAARSNSLAMITDYV
                     PGAYFVHDQLHVRGGHQVTWEVDGVEIPNTNIASNLGPQIDPKDVDYLQVERGGYGAA
                     EGDRTYGVFNVVPRTGFERNNEADLMVSGGNFGQTNDYVSLGSHTDNFAYYVSLNGNR
                     SDLGLMTPDEQIIHDAEYGFGGFSTLIENLTPQDQLRLVLSARRDDYQIPNVPGQVAG
                     DVQREADAFAILSWVRTLSSSAVLTSSLFYHYNRADYDGASWDFPISTTAQRSSSYFG
                     GEESLHWNAGRNTVDAGLYGFGQQDNQFFNVLFNDGSNPTLVQSLRPSGNVEAAWLQD
                     TYRLTESLTLSAGVRQTRFEGLTTENATDPRLGATFKLPVLGWVLRGFWGKFYQPPPL
                     ETISGPLLAFAQASALSFLPLHGERDQEYQVGLTIPVSGWAIDLDHFRTQSTNFFDHN
                     PIGNSNVFLPITITGALIAGNELTVRSPRFWGGQLVYLTYSNQTADGVGAITGGLTDF
                     SPPNGYYALDHDQRNTLNVGYRAELPWQTFISLNAYFGSGFSNAGGPPSHLPSHGTLD
                     VSLGRDFGNHLGASVTVLNVTDKHLLIDNSLTFDGFHWNYPRQVYAALHYRFSY"
BASE COUNT          576 a         1177 c         1061 g          610 t
ORIGIN      
        1 tgcgcctcgg cgacagcatc tgcgtacagg gctgctgcct caccgtgacc gagcgcgccg
       61 gacccagctt cgccgccgat ctgtcgcgcg agacgctctc gctcacgacg ctgggtgagc
      121 tcgcagtcgg cgccgcggtc aatctcgagc ccgcgctgcg cgcgggcgat gcgctgggcg
      181 gccacctcgt gtcgggacac gtcgacggca tcgcgcgcgt gaccagcgtc gagtcggacg
      241 cgcgctcgcg ccggctcgcg atcgccgtgc cgcgcgagct cgcgcgctac atcgctccga
      301 agggctcggt gacggtggac ggtgtgagtc tcacggtgaa cgaggtcgcg ggggagagct
      361 tcggcgtcaa cataatcccg cacacccaga cggcgaccac gctcggcgcg gcgagggtgg
      421 ggacgcccgt gaatatcgag gtcgaccagg tggcccgcta tctcgcacgg ctcctcagcg
      481 tgccggactg agatggggat attgcgggaa cattttgggg tgatcgaccc atgccgacag
      541 gggcattcgg cgcctagact cgagccatga gggcagggtc accgttcagg ctgacggcgc
      601 ggcagcgcac gacgctcctc gcgctcgtgc tgctgcccgc gctcgcgctg cgcgcgctcg
      661 tccccgtcgg cttcatgccg gtggtcgatg acaccggcat ccatatcggc ttctgcccgg
      721 gcgaggcaca gccgcccggc gcgctcgccg gcgccctcgc cgccggccac ggcgcgcacg
      781 ccgcgcatca tcatgaccac ggcggcgcga atcacggctc accagctccg gttagccatc
      841 cgccgtgcct gttcgccctc agcgcctccc cggctttcac gccggcagcg cccactctcg
      901 ctccgcctcc acccgcggcg gctgcgctcg ccgccgactc ctcgagccgg gtttctgtcc
      961 cggcgatcgt ccgcgctcag agctcgcgcg gcccaccctt cctcgcctga aaagctgact
     1021 cgaccttagc ggcacgcggc ctgcggaatt tcgggcgcgc gccgcggccc gctgcgcgct
     1081 tcaccgcggg cggcggtatt tcagcagatc acgcgaggaa gaataacgtg aagattcatt
     1141 tggcttcggc attcattgcc atcctagtct tgttagtttt gccgtcggtc gtgcgcgccg
     1201 ccgacctcgg cgatgtgcat ggtgtggtgc acgatgcgca gcaccgtcca gtggcggccg
     1261 cggcggttga gctcaaagcg gccacgtccg actggtcaca gacgacgcgc agcagtggcg
     1321 gtggagagtt ctctttcccc ggcgtccctt tggggaacta tctcctgacg gtcagccgca
     1381 gcggcttcgt gacggcggtg atgccgctca ccgttctgcc cggtgccgcc ccctcgacac
     1441 atgtgcagct cggcgccggc gagtctctcg agacggtcac ggtgagtgcg acggtaccgg
     1501 cgagcgccga tacgttcacg tccagcacgc tcgtcgatcg aggcgacatc cagagcactc
     1561 ccggtgccgc ccgcagcaac agcctcgcca tgatcaccga ttacgtgccg ggcgcgtatt
     1621 tcgtgcacga tcagttgcac gtacgcggtg gacaccaggt gacctgggag gtcgatgggg
     1681 tggagatccc caacaccaac atcgcgagca acctcggtcc ccagatcgat ccgaaggacg
     1741 tggactacct gcaggtcgag cgcggcggct acggtgccgc cgagggcgat cgaacctatg
     1801 gcgtcttcaa cgtcgtgccg cgcacggggt tcgagcgcaa caacgaggct gacctcatgg
     1861 tcagcggcgg taactttggc cagaccaacg attacgtcag cctgggcagc catactgaca
     1921 actttgccta ctacgtgagc ctcaacggca atcggagcga tctgggtctc atgacgcccg
     1981 acgagcagat catccacgat gctgagtatg gcttcggcgg tttctcaacc ttgatcgaga
     2041 acctcactcc gcaggatcag ttacgcctgg tcctctcggc acgccgcgac gactaccaga
     2101 tcccgaacgt gcctgggcag gtcgccggag acgtccaacg ggaagccgat gcgttcgcga
     2161 tcctgtcctg ggtgcgcacc ctgagtagca gtgcggtgct gacgagctca ctcttctacc
     2221 actataaccg cgccgactac gacggggcgt cctgggactt ccccatcagt accaccgcac
     2281 agcgctcatc gagttacttc gggggcgagg agagtctgca ttggaacgcg ggccgtaaca
     2341 ccgtggatgc cggactctat ggtttcggtc agcaggacaa ccagttcttc aacgtcctct
     2401 tcaacgatgg cagcaatccc acgctcgtgc agtcgctaag accctccggc aacgtggaag
     2461 cggcgtggct ccaggatacc tatcggctta ccgagtcgct cacgctctca gccggtgtgc
     2521 gccaaacccg cttcgagggc ctcacgacgg agaacgcaac cgatccgcgg ctcggcgcca
     2581 ctttcaagtt gcccgtcctc ggctgggttc tgcgggggtt ctgggggaag ttctatcagc
     2641 cgccaccact ggagacgatc tccgggccgc tcctcgcttt cgcgcaggct agcgcactca
     2701 gctttctccc gctgcacggt gagcgtgacc aggagtacca ggtaggcctg acgatccccg
     2761 taagcggctg ggcgatcgac ctggatcact ttcgcaccca gtcgaccaac ttcttcgatc
     2821 acaacccgat cggcaactcg aacgtattcc tgccgatcac gatcaccggc gcgttgattg
     2881 cgggaaacga gctcacggta cgctcgccgc ggttctgggg aggacaactg gtctacctca
     2941 cctactccaa ccagacggca gacggcgtgg gagcgatcac cggcggtctt accgatttct
     3001 ctccgcccaa cggttactac gctctcgacc acgatcagcg caacacactc aacgtgggct
     3061 atcgcgccga gcttccctgg cagaccttca tttccctgaa cgcgtacttc ggctccggat
     3121 tttccaacgc aggtggacct ccgagtcacc tcccaagcca tggcaccctg gacgtcagtc
     3181 tcggcaggga cttcggtaat catctgggag cgtcagtcac cgtactgaac gtgaccgaca
     3241 agcacctgct gatcgacaac agtctgacct tcgacggctt ccactggaac tacccgcgcc
     3301 aggtctacgc ggcgctgcac taccgcttca gttactgatg gtgtacgggc gcgcggcgcg
     3361 gcacacttgg gcgatgagga gaactgcaca gataggccgg ctgccagcgc tgctgctcgg
     3421 cgtc
//
LOCUS       DATLKL010000019         1506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_146747, whole genome shotgun sequence.
ACCESSION   DATLKL010000019 DATLKL010000000
VERSION     DATLKL010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146747"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..339
                     /locus_tag="VM713_00215"
     CDS             46..339
                     /locus_tag="VM713_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82715.1"
                     /translation="MKMLIRSAVAGMLLASVAFGAVSPQEAARLGQDLTPLGGEKAGN
                     ADGTIPAWTGGIKSAADAGFPNYRSGDHIPTHTRATSRCSPSRRRTSASTPPN"
     gene            357..1409
                     /locus_tag="VM713_00220"
     CDS             357..1409
                     /locus_tag="VM713_00220"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HVH82716.1"
                     /translation="MFKTYPDYKMIVYPTRRSAASPEHVYEATKRNATTGKLVPDGNG
                     ITGAIAGVPFPIPQSGVEIYWNHVTRWRGLAAALEVGQAPLTASGSFTLVNFKEEFYF
                     QYYEPGMTEAKMNNILLYFTQETTAPARLAGEILLVQETLDQAKEPRRAWVYNPGQRR
                     VRRAPNVAFDNPGTNADNLRTADQFDMYNGSPQRYDWTIVGKKEMYVPYNAYRLQDPK
                     IKYSQMLPKNHLNQDLARYELHRVWVVDSKLKPGASHLYSRRTLYVDEDSWQILAVDC
                     YDARGQLYRVQEGHAMNYYDLPSEWTTLELVMDLSNGRYLALGLQDEEPHSYSFNVKR
                     SPDDYQPSMLERRGIR"
BASE COUNT          297 a          508 c          471 g          230 t
ORIGIN      
        1 cctgcgacgg gtttgtaccg ggaacgcata ttgaaggggg gcgccatgaa aatgctgatt
       61 cggtctgccg tcgcaggaat gctgctcgca tccgtggcgt ttggtgccgt gagcccgcag
      121 gaggccgcgc ggctcggcca ggacctcacc ccgctcgggg gtgagaaggc cggcaacgcc
      181 gacggcacga ttcccgcctg gacgggcggc atcaagtcgg cggcggatgc gggttttccg
      241 aactaccgct ccggcgatca catcccgacc catacgcgag cgacaagccg ctgttcacca
      301 tcacgacggc gaacatcagc cagtacgccg ccaaactgac cgagggtcac aaggcgctgt
      361 tcaagactta ccccgactac aagatgatcg tgtacccgac gcgccgcagc gctgcatcgc
      421 cggagcacgt gtatgaagcg accaagcgca atgccactac gggcaagctc gttcccgacg
      481 gcaacggcat caccggggcg atcgccggcg tgccgttccc gattccacag tcgggagtcg
      541 agatctactg gaaccacgtg acgcgctggc gcgggctcgc cgcggcgctc gaggtcggcc
      601 aggcgccgct cacggcgagc ggcagcttca ccctggtgaa cttcaaggag gagttctact
      661 tccagtacta cgagcccggg atgaccgagg cgaagatgaa caacatcctg ctgtacttca
      721 cgcaggagac caccgcgccg gcgcgcctcg ccggagagat actgctggtg caggagacgc
      781 tcgatcaggc caaggaaccg cgccgcgcct gggtctacaa cccgggccag cgccgcgtgc
      841 ggcgcgcgcc caacgtggcc ttcgacaacc cgggcaccaa cgccgacaac ctgcgcaccg
      901 ccgaccagtt cgacatgtac aacggcagcc cgcagcgcta cgactggacg atcgtcggca
      961 agaaggagat gtacgtcccg tacaacgcct atcgcctgca ggatccgaag atcaagtaca
     1021 gccagatgct gcccaagaat cacctcaacc aggacctcgc gcgctatgag ctgcaccgcg
     1081 tgtgggtcgt ggactcgaag ctcaagcccg gcgcgagcca cctctacagc cgccgcaccc
     1141 tgtacgtgga cgaggattcg tggcagatcc tcgcggtcga ctgctacgat gcgcgcgggc
     1201 agctctaccg cgtccaggaa ggacacgcga tgaactatta cgatctgccc tcggaatgga
     1261 cgacgctcga gctggtaatg gatctctcca acgggcgcta tctggccctc ggcctgcagg
     1321 acgaggagcc gcactcctac agcttcaacg tcaagcgctc gcctgacgat tatcagccga
     1381 gcatgctgga gcgccgcggc atcaggtaaa cgccgcgagc gccaaacgcg tttttttggg
     1441 gccgccgtga atcctttagc gctcgcggct gcgctcgcgg ttagtgcggc cctcgcggcc
     1501 gctgcc
//
LOCUS       DATLKL010000020         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_101718, whole genome shotgun sequence.
ACCESSION   DATLKL010000020 DATLKL010000000
VERSION     DATLKL010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101718"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..812)
                     /locus_tag="VM713_00225"
     CDS             complement(102..812)
                     /locus_tag="VM713_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82717.1"
                     /translation="MKPAAPARSGRVFAVLTGDIVGSRQLSAERLERTRRVIAAGAAQ
                     FRRRERRALCGVPEVFRGDAWQLLLREPRWALRVALLVQAQLLARNDVRTRISIGVGG
                     VDVINRRRISLSTGEAFMLSGHALDHITGYFDLTGALPERTAPLAAWFAALLHLCSGL
                     VRPWTRRQAEIVSLALLADNPTHESIAGTLRPRVSKQSVTESLAAAHWRPLLEGIRVF
                     EETDWNGLVARRQAEAIV"
     gene            complement(887..1342)
                     /locus_tag="VM713_00230"
     CDS             complement(887..1342)
                     /locus_tag="VM713_00230"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HVH82718.1"
                     /translation="METDLAALVRESLAAAAAAMREVPMFGGIGFLLNGNLLAGASRR
                     GLLLRVGPERAAEALARPGARPLVMRGRTLEGYVYVDPPALTAAAVRGWIELALPHVL
                     ALPPQAAKKRARKAARAAGSAKAGRKKAARRRPVRETRQRARRRPARAK"
     gene            complement(1375..>2183)
                     /locus_tag="VM713_00235"
     CDS             complement(1375..>2183)
                     /locus_tag="VM713_00235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HVH82719.1"
                     /translation="VCELRSVNHRFLEAGFRLPDELRSAEGELRSRLAQKVRRGKVDC
                     TMSYRRPAGASGTFEVDARALERLLHAAQTVSRSLHGPATLNVLDVLRWPGVLREDAV
                     DGEPLLAAAGELFATTLADLSAARAREGQRLRELLEQRCGVLEGLIATVRARVPEVQA
                     RARTRLAERLAELTAGIDHERIEQELALLLQRLDVDEELERLTGHVAEVRRVIGAGEP
                     AGRRLDFLMQELNREANTLSSKSQDLETTRSAVDMKVVIEQMREQVQNAE"
BASE COUNT          320 a          796 c          761 g          306 t
ORIGIN      
        1 aagtccgcga ccaggtgcgc gaccagcagc gccagcaggg tttcatacat gagttgcgca
       61 gctcccgagt cctaagggca ggttaatgag cctgtcaccg ttcagacaat cgcttcagcc
      121 tgtcgtcgcg cgacgagtcc attccagtcc gtctcttcga agacccggat gccttcgagc
      181 agcgggcgcc agtgcgccgc cgccagggac tccgtcacgc tctgcttcga gaccctggga
      241 cgcagtgtgc cggcaatcga ttcgtgggtg ggattgtccg ccaggagcgc caggctcacg
      301 atctcggcct gccggcgggt ccagggacgg accaggccgc tgcacagatg caacagcgcc
      361 gcaaaccagg cggccagcgg agcggtccgc tcgggcaacg cccccgtcag gtcgaagtaa
      421 cccgtgatgt ggtcgagcgc gtggcccgag agcatgaagg cctcgcccgt cgagagggag
      481 atgcgcctgc ggttgatgac gtcgacgccg ccgacaccga tggagatgcg ggtccgcacg
      541 tcgttcctgg caagcaactg cgcctgcacg agcagcgcca cccgcagcgc ccagcgcggc
      601 tcgcgcagca gcagctgcca ggcgtcgccg cgaaagactt ccggcacccc gcagagggcg
      661 cgccgctcgc gccggcggaa ctgcgcggcg cccgcggcga tgacgcggcg cgtgcgctcc
      721 agccgttcgg ccgacagctg ccgtgatccg acgatatcgc cggtgagcac ggcgaacacc
      781 cggccgctgc gggcgggagc ggcgggtttc acgggcggct ggaatccaga caggccataa
      841 tgcctgtctg gtataagaca ggttttttac cctgtcaagt ccccggtcac ttcgcccgcg
      901 ccggccggcg gcgcgcgcgc tggcgtgtct cgcggacagg tctcctgcgt gcggctttct
      961 tccggccagc cttggcggag ccggcagcac gcgccgcctt gcgggcgcgc ttcttcgcgg
     1021 cctgcggcgg gagcgcgagc acgtgcggca gcgccagctc gatccagcca cgcactgccg
     1081 cggcggtgag cgccggcggg tcgacgtaga cgtagccctc gagggtgcgc ccgcgcatca
     1141 ccagcgggcg tgcgcccggg cgggcgagcg cctcggccgc gcgttcgggc ccaacgcgca
     1201 gcagcagccc gcggcgcgag gcgccggcga ggaggttgcc attcagcagg aagccgatgc
     1261 cgccgaacat cggcacctcg cgcatcgcgg cggcggcggc cgccagactc tcacggacca
     1321 aggcggcaag atcggtctcc acagcctgag ctccctccag cggtctcttg ccgctcattc
     1381 ggcgttctgc acctgctcgc gcatctgctc gatgaccacc ttcatgtcga ccgccgagcg
     1441 cgtggtctcg agatcctgcg acttcgagga cagggtattc gcctcgcggt tgagctcctg
     1501 catgaggaag tcgagccggc gtccggcggg ctcgccggcg ccgatcacgc ggcgcacctc
     1561 ggcgacgtgg ccggtgaggc gctcgagctc ctcgtcgacg tcgagccgct gcagcagcag
     1621 cgccagctcc tgctcgatgc gctcatgatc gatcccggcg gtgagctcgg cgaggcgctc
     1681 ggcgagacgc gtacgggcgc gcgcctgcac ttcgggcacg cgcgcccgca ccgtggcgat
     1741 gagtccctcg agcacgccgc agcgctgctc gagcagctcg cgcagccgct gcccctcgcg
     1801 cgcccgtgcc gcggagagat ccgcgagcgt cgtcgcgaac agctcacccg cggctgcgag
     1861 cagcggctcg ccgtccaccg cgtcttcacg cagcacgccc ggccagcgca gcacatcgag
     1921 cacattgagc gtcgccgggc cgtggagcga gcggctgacg gtctgcgccg cgtgcagcag
     1981 acgctcgagc gcgcgcgcgt ccacctcgaa cgtcccgctc gcgcccgcgg gacggcggta
     2041 gctcatggtg cagtcgacct tcccgcggcg tactttctgc gcgagccgcg agcgcagctc
     2101 gccctcggcc gagcgcagct cgtccggcag gcggaagccc gcttccagaa agcggtgatt
     2161 cacgctgcgc agctcgcaca cca
//
LOCUS       DATLKL010000021         1697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_180640, whole genome shotgun sequence.
ACCESSION   DATLKL010000021 DATLKL010000000
VERSION     DATLKL010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180640"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /gene="crcB"
                     /locus_tag="VM713_00240"
     CDS             <1..292
                     /gene="crcB"
                     /locus_tag="VM713_00240"
                     /inference="COORDINATES: protein motif:HMM:NF010802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HVH82720.1"
                     /translation="TIVVNVTGCFVIGFFATLSGPDGRLLAPSSVRQFVMTGICGGYT
                     TFSSFALQTLNLARDGEWSGAGANVALSVVLCLLAVYAGHVAAALLNQLARG"
     gene            296..652
                     /locus_tag="VM713_00245"
     CDS             296..652
                     /locus_tag="VM713_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HVH82721.1"
                     /translation="MPIPRDALLLRIFVGEDDRHDHKPLYEAIVLKAREHHIAGATVL
                     RGPMGFGHSSRIHTTKILRLSEDLPLVIEIVDSEDKINAFLPVLDDMMGSGLVTLEKV
                     RVLRYGVAEAVPSLGA"
     gene            806..1024
                     /gene="infA"
                     /locus_tag="VM713_00250"
     CDS             806..1024
                     /gene="infA"
                     /locus_tag="VM713_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HVH82722.1"
                     /translation="MAKEDLIEVAGTVTELLPNAMFRVKLDNEHEILAHTSGKMRKNR
                     IRVLAGDRVNVEMTPYDLTKGRITFRFK"
     gene            1053..1640
                     /locus_tag="VM713_00255"
     CDS             1053..1640
                     /locus_tag="VM713_00255"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HVH82723.1"
                     /translation="MANARAVPRLVLASASPRRLELLRQIGIEPAAIAPAAIEEHALP
                     REPPRAHALRLAEAKARAVASGHPGAYVLGADTVVACGRRILPKALDEATARRCLALL
                     SGRRHRVLGGLCVIAPGGRHACRLVTSTVAFRRLQVDDIDAYIAAGEWHDKAGGYAIQ
                     GRAAAFVRHFSGSYTNVVGLPLFEAAALLRGLGYR"
BASE COUNT          270 a          581 c          549 g          297 t
ORIGIN      
        1 aaccatcgtg gtcaatgtga ccggatgctt cgtgatcggc ttcttcgcga cactgagcgg
       61 accagacggt cgcctgctcg cgccgtcctc ggtccgccaa ttcgtgatga cgggcatctg
      121 cggcggctac accactttct cctccttcgc cctccaaacg ctcaacctgg ctcgggacgg
      181 cgaatggagc ggcgccggcg caaatgtggc gctctcggtg gtgctgtgcc tgctcgcggt
      241 ctatgctggg catgttgccg ccgccctcct caaccagctc gcaagaggtt gacccatgcc
      301 gatcccgcgc gacgcgctgc tgcttcgcat cttcgtgggc gaggacgacc gccacgatca
      361 caagccgctc tatgaagcaa tcgtgctcaa ggcccgcgaa caccacatcg cgggtgcaac
      421 ggtgctgcgc ggtccgatgg gcttcggtca ttccagccgg attcatacaa cgaagatact
      481 gagactgtcg gaagacctgc cgctggtgat cgagattgtc gacagcgaag ataagatcaa
      541 tgccttcctt cccgtgctcg acgacatgat ggggagtggg cttgtgacgc tcgagaaggt
      601 gcgcgtactc aggtacggcg tcgccgaggc ggtgccaagt ctcggcgcgt gaagcggcga
      661 ggcgccccat tttcgcggtc gcggcgacgg cgaaccccgc ttccacgtgc ccgcagctga
      721 gccgcaatgt cgggcgaaaa tgcttgaccc ccgacggcgt tgcgctccat tttctccctc
      781 gccgaaggca agcttggtga gggtaatggc gaaagaggat ctgatcgagg tcgccggcac
      841 ggtaactgaa ctgctgccga atgcgatgtt ccgcgtgaaa ctggacaacg agcatgagat
      901 actcgcccac acctccggca agatgcgcaa gaaccgcatc cgcgtgctgg cgggcgaccg
      961 cgtgaacgtc gagatgacgc cctacgatct gaccaagggg cgcattacct tccgcttcaa
     1021 gtagcccggc gctcgccgaa gcgccccctc ccatggcgaa cgcccgagcg gtgccgcgct
     1081 tggttctggc ctcggcgtcg ccccggcgcc tcgagctttt gcgtcagatc gggatcgagc
     1141 ccgccgcgat cgcgcccgcc gcaatcgaag agcacgcgct gccgcgcgag ccgccgcgcg
     1201 cccacgcgct ccgcctcgcg gaggccaagg cccgcgccgt cgcatcgggg catcccggtg
     1261 cttacgtact cggcgccgac acggtggtcg cctgcggccg gcgcatcctg cccaaggcgc
     1321 tcgacgaggc gaccgcgcgg cgctgcctgg cgcttctctc cggccgccgc catcgtgtgc
     1381 ttggcggatt gtgcgtgatc gctccgggcg gacgccacgc gtgccggctg gtcacctcga
     1441 ccgtcgcgtt caggcgcctt caggtggatg acatcgacgc ctacatcgcg gcgggcgagt
     1501 ggcacgacaa ggcgggaggc tacgccattc agggccgcgc cgccgccttc gtgcgccatt
     1561 tctccggctc ctatacgaac gtggtcggcc tgccgctctt tgaggcggcg gcgctgctcc
     1621 gcgggctcgg ctaccggtga gcgccgagtc ctcgatcgac gaaattgtct tcaacgtctc
     1681 gcccgacgag acgcggg
//
LOCUS       DATLKL010000022         5323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_22750, whole genome shotgun sequence.
ACCESSION   DATLKL010000022 DATLKL010000000
VERSION     DATLKL010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22750"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VM713_00260"
     CDS             <1..405
                     /locus_tag="VM713_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82724.1"
                     /translation="MMQIPGKLLIATVLATAGSAARADDLYIVCNLRPAITAADVRDL
                     FVGEKQFVGAVKLVPIDNSAAQGLFLERVLKMTAARYSVTWIKKSFRDGINAPVVAGS
                     DAEALATVKRTPGACSYVTTPPDANLEVIAKL"
     gene            512..1951
                     /locus_tag="VM713_00265"
     CDS             512..1951
                     /locus_tag="VM713_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HVH82725.1"
                     /translation="MAKRKLIETRSIDYVPLAERHGKVWHLWPVWFSGDAHLATLAVG
                     VLGITLGGNLLWTAVAVLLGNALGTFFMAFHSTQGPQLGLPQMIQSRPQFGYTGALLV
                     WAVALVAYIGYNAFNQVLAAQALHQLTPAISATSPGAIIVFALVAVSLAAVGYDQIHR
                     AQRALAYLMIAILTVFSLGGLATLRFSAAQWATGGFRVVPFLAQLFAAASYQLSWSIY
                     VSDYSRYLPRDVGVRESFWWTYLGAFIGGAWMMLVGTVAAAMAPRLDVAAAMERAADA
                     VIPGLGKVLIAGALLGLITITSLNFYGASLTLLSVADTVRPLKCTAAKRLASLGCALG
                     ASTLLAVNSSGNFVARFQDYLALLLYLFTPWTAINLVDFYLVRKGHYSIREIFNSRGM
                     YGHWNWRGLSAYLIGFMVMIPFFSTELYSGPVARALGGADIAMLVGLPVAAGVYLLTC
                     RSLDLEHDRRRATAADVGLDPDDRVGAPG"
     gene            1961..2599
                     /locus_tag="VM713_00270"
     CDS             1961..2599
                     /locus_tag="VM713_00270"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HVH82726.1"
                     /translation="MPKIVDHSQRRDEIALVACRVVAEHGFDQASMVRIAREAGYTTG
                     MLAHYFDSKQDIIIAALRLILRRIEERLMRPAPDGATPDLLTLLTEALPVDAHRRIEC
                     AFWTTFWGQVSADKRLKRINAWVHREYLRLFERCLALCWSEWANWPAATREHVLRSLV
                     TFINGLTASAVANAGDWPARAQTEQLRLQLALLHAWAEGSAGAPHARRTAAD"
     gene            complement(2629..5097)
                     /locus_tag="VM713_00275"
                     /pseudo
     CDS             complement(2629..5097)
                     /locus_tag="VM713_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(5141..>5323)
                     /locus_tag="VM713_00280"
     CDS             complement(5141..>5323)
                     /locus_tag="VM713_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVH82727.1"
                     /translation="SADPIMDEELRATLREALPQAEVKIFPGLGHNPFWEQPAQCAAV
                     INEFLTAAPAGHANSF"
BASE COUNT          818 a         1791 c         1791 g          923 t
ORIGIN      
        1 atgatgcaaa tcccgggcaa acttctcatt gcgaccgtgc tcgccacggc gggcagcgcc
       61 gcccgtgccg acgatctcta catcgtctgc aacctgcggc cggcgatcac cgccgccgac
      121 gtgcgcgacc tgttcgtcgg cgagaagcag ttcgtcgggg cggtgaagct ggttcccatc
      181 gacaacagcg ccgcgcaggg cctgttcctc gagcgggtcc tgaagatgac ggccgccaga
      241 tattcggtca cctggatcaa gaagtcgttc cgggacggca tcaatgcgcc ggtcgtcgcc
      301 ggcagcgacg cggaggccct cgctaccgtg aaacgcaccc cgggggcctg cagctacgtc
      361 acgacgccgc cggatgccaa cctggaagtg atcgccaagc tgtagcggcc cgccccccgc
      421 ccgggcgcac ccggccgcga aggccgcgcc cctataatgt tggcgccgtg atataaaaag
      481 cggccaacgc aggccgcgga ccttcttcgg tatggctaag cgcaaactca tcgagacgcg
      541 ttccatcgac tacgtgccgc tcgccgagcg gcacggcaag gtctggcatc tgtggccggt
      601 gtggttctcg ggcgatgcgc acctcgcgac gctcgcggtc ggcgtgctcg gcatcacgct
      661 cggcggcaat ctcctctgga ccgccgtcgc cgtgctgctc ggcaatgcgc tcggcacctt
      721 cttcatggcg ttccactcga cgcaggggcc gcagctcggg ctcccgcaga tgatccagtc
      781 acggccacag ttcggctaca cgggagcgct gctcgtctgg gcagtcgcgc tcgtcgccta
      841 catcggctac aacgccttca accaggtgct cgccgcacag gccctgcatc agctgacgcc
      901 ggccatctcc gccacctccc ccggcgccat catcgtgttt gccctggtgg cggtgagtct
      961 cgcggcggtc ggctacgacc agatccatcg cgcccagcgc gccttagcct acctcatgat
     1021 cgcgattctc acggtgttct cgctcggcgg cctcgcgacg ctgcgcttct ccgcggcgca
     1081 gtgggcgacc ggcggctttc gggtcgtgcc gttcctcgcg cagctgttcg ccgcggcttc
     1141 ctaccagctg agctggtcga tctacgtatc ggactactcc cgctatctgc cgcgcgatgt
     1201 cggcgtgcgc gagtcgttct ggtggactta cctcggcgcc ttcatcggcg gcgcctggat
     1261 gatgctggtc gggaccgtgg cggcggccat ggcgccccgg ctcgacgtgg ccgctgccat
     1321 ggagcgggcg gcggatgcgg tcattccggg cctcggcaag gtgttgatcg ccggagcgct
     1381 gctcggcctc atcacgatca cctcgctcaa cttctacggc gcctctctga ccctgctctc
     1441 ggtggccgac accgtgcggc ccctcaagtg cacggccgcc aagcggctgg cgagcctcgg
     1501 ttgcgcgctc ggcgcgagca ccctgcttgc cgtgaattcc tcggggaatt tcgtggcgcg
     1561 ctttcaggat tacctcgcgc tgctcctcta cctgttcacg ccctggaccg cgatcaacct
     1621 ggtggacttc tacctggtgc gcaaggggca ctactcgatc cgcgagatct tcaactcccg
     1681 cggcatgtac ggccactgga actggcgcgg actcagcgcc tacctcatcg gcttcatggt
     1741 gatgatcccg ttcttcagca ccgagctcta ctcgggcccg gtggcccgcg ccctcggcgg
     1801 cgccgacatc gccatgctgg tcggtctccc ggtcgcggcc ggcgtctatc tactcacctg
     1861 ccgctccctg gatctcgagc acgaccggcg acgggccacc gccgccgacg tcggcctcga
     1921 tcccgacgac cgggtgggcg cgccagggta ggatcgggac atgcccaaga tcgtcgacca
     1981 ctcccagcgc cgtgacgaga tcgccctcgt cgcctgccgg gtggtggccg agcacggttt
     2041 cgatcaggcg agcatggtgc gcatcgcccg cgaagcgggc tacaccacgg gcatgctcgc
     2101 gcattacttc gacagcaagc aggacatcat catcgcagct ctgcggctca tcctgcggcg
     2161 catcgaggag cggctgatgc gcccggcacc cgacggtgcg acgcccgatc tgctcaccct
     2221 cctgaccgaa gcgttgccgg tcgacgcgca ccggcgcatc gagtgcgcct tctggaccac
     2281 gttctgggga caggtctccg ccgacaaacg cctcaagcgc atcaacgcct gggtgcaccg
     2341 cgagtatctg cggctcttcg agcgctgcct cgcgctgtgt tggtccgaat gggcgaactg
     2401 gccggccgcg acgcgcgagc atgtgctgcg ctcgctggtc acgttcatca acgggctgac
     2461 ggcgagtgcg gtcgccaacg ccggcgactg gcccgcgcgc gcgcagaccg agcagctgcg
     2521 gctgcaactc gcgctgctgc acgcctgggc cgaaggctcg gctggcgcgc cgcacgcgcg
     2581 tcgcacggcg gccgactgag gccgccggtc gccgccgatc gcccgccgtc agaagctctt
     2641 gctgagctgc agcgtcacgg tgagcggcag cagggtcgct acctgcggaa tcacgctgcc
     2701 cgcggcgttc agctgcgcat agccgatgta gccgatgtca ccgagattcg gtttggcgtt
     2761 gaagaggtta tgcacgttga gggaggcctc gatgtccccg cgatcgatgc cgaagcgcag
     2821 attgacgagc gagaagcctg gccgtgtcgc ctcagcgaag gcgccgctct ggagcagcga
     2881 gatggcgttg ccctggtagc tgtagtcggc cgacacgaag ccctgggtgt cggcggcgag
     2941 gggatgcgtg tagacgaggc ccgcggaccc ggtgaactgc ggcacgcccg aaacgcgtgt
     3001 gccggggctg acgccgaaca gtgcgaggtt ccctgggtcc gtcatgtccg tatcctcgta
     3061 cccgaggcct aggcggaact gcaagccagg cagtccgagg cgcccgctcg cctagagctc
     3121 ggcgccgcgc acgcgcgcat ggttgccgtt cacctgcgcg tagaacccgc agaccagcga
     3181 cacctgctgc tgcacgttgc tccactcgat gtcgtaagcg gccgccgaga tcagcatggc
     3241 gggcagctgc gacttggcgc cgagctcgta ggtccagagc gtatccgaac ggatgtgggt
     3301 gatcgcatcc accgacaggc tcggcagcgt gcagccggga aagttggcct gcgcaccgcc
     3361 ggagcggaag cccttcgagg ccgatgcgta ggtcatcgtg ctgtcagtca cctgccatgc
     3421 cactgccacc ttcggattga aacccgactc gctgttggac tgggggctgc tcggcgtcgg
     3481 cccgccgttc aggaagccgt cggccgaata gtcagtcgtc tggttcaacc agtactggcg
     3541 tcccccgagg gtgagcgtcc actgcggcgc gaacttccag tagacctcgc cgaagatcga
     3601 cttgtcctcc tcggtccccg gattggtctg cgtccagatc tcggggctcg accaagcggg
     3661 cgacaggtac acgggatttg ccggcgtgca cggcggcggc agtgtgccga actcgcagga
     3721 ggcggtccct gccgcgttga tgccgatgga atcggtctcc gggatggtga agcgcgtgtg
     3781 cgtgttggaa tagaacaggc cgacggtgcc gctgaagtta ttgaccggat cccaggagaa
     3841 gcgcgcctcg ctcgagagct ggtcgtgatg gcggtcgccg atccacagat acggctgggg
     3901 cgcgaccccc gtcagctggt agagggggct ggtgagaatc tgctgcgtgc cgtagctcga
     3961 gtcctcgacg tcataggtgt ggcggtagaa gtagctattg gcccacacga aggtccagcc
     4021 gctgcccgag tacttgatat cgagcgacgg cagggtccag gtatcggtcg catagggctg
     4081 aacgttgaag gcgcgagcca gcgaatactg cggggaagtg aagttcgtgc cggccgaatt
     4141 gagcggcgcg aaggtcgccg ggaaaccgtg gtccttcgaa tcctggtaca gcaggcgcac
     4201 cttgatgtcg agcgcgtcgg tgggcttgac gagcagcgcg agcgagccgc cgccggtgga
     4261 cacggccccc tgatcccccg agctgccgcg cggcacggtc tcgaacgggt caccgcccgc
     4321 ggggtcgcca gccggtgcca gcgggttgag cggaaaggtg cgggtgagat aaccggcgtc
     4381 gtgattgagg aacagcacga cgcgcagcgc cgcactgccg gggactacaa ccaggttgtt
     4441 gatgaagttg ccgccaccgt cggggctgcc gccgccagaa gtgagaccgg cgtcgaccat
     4501 gtagccgaag ccgctgctgt tgaggtccgg ctgcctggtg atcagccgca cgttgccacc
     4561 gagggagctc tcgccgtaga gggtgccctg cgggcccttc agcacctcga tgctggcgag
     4621 atccaccacg cgcgggtcga gcgagcccgg caccggcgtg tcgtcgatgt agaaaccggt
     4681 ggcgcctgca gtgccgaaga ggttttggcc ggtaatgccg cgaaccgcga cggtgcgcga
     4741 gtcggcgatg ccggtcggac cgccgccgta ggtgaacgac aggtctggag tcttggtggc
     4801 gtagtcggca aacgactgga cgttgtagtc cttgatggtc gcggccgtga acgctgtgat
     4861 gctggtcggc acctcggcga tcgactccgc gcgcttgcgg gcgctgacga tgatggtggc
     4921 gacctcgccg ctcgcctcgg cgccgctctc cgcctgcttc tgcgactgct cctgggcgcg
     4981 agcgggcggc gtgagcgcga ggcacagtgc gcccaacgtg ccggctgcca gcgcgccggc
     5041 gccctgcgtc cctgagaacg cgcgctttgc tccgtaacgg gatccgttgg atttcatttg
     5101 ccccctcccc tgagtgaact tggcagcgga tcggcttcct tcagaaagag ttcgcgtgtc
     5161 ccgccggcgc tgccgtcaga aactcattga taaccgcagc gcactgcgcc ggctgctccc
     5221 agaacgggtt atggcccagc ccgggaaata tcttcacttc ggcctgtggc agagcctcgc
     5281 gcagcgtcgc acgcaactcc tcatccatga tcggatcggc act
//
LOCUS       DATLKL010000023         4247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_113004, whole genome shotgun sequence.
ACCESSION   DATLKL010000023 DATLKL010000000
VERSION     DATLKL010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113004"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..3390)
                     /locus_tag="VM713_00285"
     CDS             complement(208..3390)
                     /locus_tag="VM713_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82728.1"
                     /translation="MTVGVAAAADADKEGDFKFRFVGPHVGNRIAAVAGVPGDTSVYY
                     AGAASGGVWKSSDGGNRWAPVFDKQNVAAIGALAVAPAEPGTVWAGTGEAWVIRDSDV
                     MGNGIYKSTDAGQTWTNMGLSESGRIGRIVVHPSNPDIVFACVLGRVTGPQQERGVYR
                     TTDGGQHWARVLFAGENVGCSGLSLDAHNPHTLIAGMWQVEMHTWGEYSGGPGSGIYL
                     SHDGGTTWKRLEKDGLPDGPLGKIDVAIAPTNSKRIYALIQTKDQGSVWRSDDAGKQW
                     KLVNSQRALIGRAGYYIRLAVSSGTDSEVYVANSSFHQSLDSGESFHEVRWGGDTHDI
                     WVDPLNPDRFVITDDGGMIITAVHGRGFHRVQLPIGQMYHVAVDEQIPYYFYSNMQDD
                     GNMRGPSLALGGEEGWDRHMGGCESGFTIPDPADPNVVWATCYGDTVTRWDARYREAH
                     SVSPWKHTLDSPPNAIKYRCHWTPPLAIDPFDHNTVYYGCQVIFRTTDAGHGWSVVSP
                     DLSTRDPAHIAPSGGIVGDNLGQFYGEVVFAIAPSKVQRGLIWAGTNDGQIWYTKDGA
                     ENWVNVTSHVAGLPPGGTITSIAPSSFDAATAYVSVDFHLMDNRDPFIFKTSDFGKTW
                     KRISSNLPKHELSYVRRVTDDPDCQGLLFAGTGNGLYYSLDDGGHWTALRNGLPPAPV
                     TWAVVQKEFHDLVVSTYGRGLYILDDITPLEQMARHRSDAAVVLFEPRKTYRFVAAGA
                     AFLNFSLATVPKDPVHLEILDAEGHVVRKLEAKEPVAGLNRVSWDLRYESPRLVALRT
                     LAPDNRHIWQEPRFRDEDSRPITHWGSKPAEVGPLVAPGSYSVRLKTAGQTLTQPLTV
                     VSDPRAPGSAADIQLSVKTLVRIRDDISHVSDSVNQVEWLRKQLEVIEAMLRPAKAHG
                     KQEAVLAEEGDEAEEEPAAAPDRELRAAEQQQRGELLKSTESLDQKLQAAESRLVSPA
                     LRDSDDKYFVEADGTYLDLIWLNAEVGTGGGDVAGGADFAPTLAQLDTLKTLEGEISA
                     VDGELKEILGHELPVLDSALERASLAPLAR"
     gene            3546..>4247
                     /locus_tag="VM713_00290"
     CDS             3546..>4247
                     /locus_tag="VM713_00290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVH82729.1"
                     /translation="MNRRVALVTGGGRGIGREAALLLSGAGARVMTVARTAAELATVG
                     LEYVAADLGTREGCALAVAETEQRLGPVDILVVNHGIGSAHERLVWEQDPEVWRETMR
                     INLDGPFELARLTVGGMCKRGFGRLVFTSSTAGEKAERAGSAYTASKHGLIGLARAIA
                     QDAGPFGVTSNAVLPGWVRTAMAERSAQTEAERRGVSVEQVWRERAAIYPQNRVLEPC
                     EVAQVIAFLCSDAAGG"
BASE COUNT          688 a         1370 c         1447 g          742 t
ORIGIN      
        1 ccggtggttg cggcgtgact ggcctcacaa atacccgcgt cgattctgtt aggatgacaa
       61 tcggttacag cttttaatcg ctcggcgaag tacatgaacg tacgaaagcg aagagcgcgc
      121 gcgcggagga aggccaaggc caaccgcctg gcgcgttggc aaggcgttcc gctgtacgcc
      181 tgaggaacgc agcctccggc accgcggtca gcgggccaat ggggcgaggc tcgcgcgctc
      241 gagcgcggaa tcgagcaccg gcagctcgtg tcccagaatc tccttcaact ccccgtcgac
      301 tgccgagatc tcgccctcga gtgtcttgag cgtatcgagc tgggccagag tgggcgcgaa
      361 gtcggcgcct ccggctacgt cgccgccgcc ggttccgacc tcggcgttga gccagatgag
      421 atcgagatag gtgccgtcgg cctcgacgaa gtacttgtcg tcgctgtcgc gcagcgcggg
      481 cgagaccaga cgcgattcgg cggcctgcag cttctggtcg aggctctcgg ttgacttcag
      541 cagctcgccg cgctgctgct gctcggcagc acgcaactcg cggtccggcg cagccgcagg
      601 ctcctcctcc gcctcatccc cctcctcggc gagcaccgcc tcctgcttgc cgtgcgcctt
      661 cgccggccgg agcatcgcct cgatgacctc gagctgcttg cgcagccact ccacctggtt
      721 cacggagtcg gaaacgtggc tgatgtcgtc gcggattctg actagtgtct tgacggagag
      781 ctggatgtcc gccgccgagc caggcgcgcg cggatcgctc accaccgtaa ggggctgggt
      841 aagtgtctgg ccggctgtct tgagacgcac cgagtagctg ccgggggcca ctagaggccc
      901 gacttcggcc ggcttagagc cccagtgcgt gatgggccgc gagtcctcgt cgcggaagcg
      961 cggctcctgc cagatgtggc gattgtcggg cgcgagcgtg cgcagcgcca cgagccgtgg
     1021 tgactcgtaa cgcaggtccc agcttacgcg gttgaggcct gcgaccggct ccttcgcctc
     1081 gagcttgcgc acgacgtggc cctcggcatc gaggatctcg aggtgcacgg gatccttcgg
     1141 taccgtggcc agcgagaaat ttaggaacgc cgcgccggcc gcgacgaagc ggtaggtctt
     1201 gcgcggctcg aacaggacca ccgcggcatc ggagcggtgc cgcgccatct gctcgagcgg
     1261 cgtaatgtca tcgagaatgt agagtccgcg gccgtaggtg gacacgacca gatcgtgaaa
     1321 ttccttctgc actaccgccc aggtgacggg cgcaggcggc aggccgtttc ggagcgccgt
     1381 ccagtgcccg ccatcgtcca gcgagtaata gaggccattg cccgttccgg cgaacaacaa
     1441 tccctggcag tcggggtcgt ccgtgaccct gcgcacgtag gacaactcgt gcttcggcag
     1501 attgctgctg atgcgtttcc aggtcttgcc gaaatcgctg gtcttgaaga tgaagggatc
     1561 gcggttgtcc atgagatgga aatcgacgct gacataggcg gtggcggcat cgaagctcga
     1621 gggtgcgatg ctcgtgatcg ttcccccggg aggcaggccg gccacgtggc tcgtgacatt
     1681 cacccagttc tcggccccat ccttggtgta ccagatctgt ccgtcgttgg taccggccca
     1741 gatgaggccc cgctgaacct tcgacggcgc gatcgcgaac acgacctcgc cgtagaactg
     1801 cccgaggttg tcgccgacga tgccgcccga cggagcgata tgcgccggat cccgggtgga
     1861 cagatcgggg ctgaccaccg accagccgtg acctgcatcg gtcgttctga agatcacctg
     1921 gcagccgtag tagaccgtgt tgtggtcgaa gggatcgatg gcgagcggcg gcgtccagtg
     1981 gcagcggtac ttgatggcat tgggcgggga gtcgagcgtg tgtttccagg ggctcaccga
     2041 atgcgcttcc cggtaacgcg catcccagcg cgtcaccgtg tctccgtaac aggtggccca
     2101 cacgacgttg gggtccgcgg gatcgggaat cgtgaatccg gactcgcacc cgcccatatg
     2161 gcgatcccag ccttcctccc cgccgagggc caggctcggg ccgcgcatgt tcccgtcgtc
     2221 ctgcatgttg ctgtagaagt agtaggggat ctgctcgtcc accgccacgt ggtacatctg
     2281 gccgatgggg agctggacac ggtgaaagcc gcggccgtgg accgccgtga tgatcatgcc
     2341 gccgtcatcc gtgatgacga agcggtcggg gttcagtgga tcaacccaga tgtcgtgcgt
     2401 gtcgccgccc cagcgcactt catgaaagct ctcgcccgaa tccagtgact gatgaaacga
     2461 gctgttggcg acgtagactt cgctgtccgt tcccgacgat acggcgaggc ggatgtaata
     2521 gcccgcgcgt ccgataagcg cccgctggga attgaccagc ttccactgct tgccggcatc
     2581 gtcggagcgc cacaccgatc cctggtcctt ggtctggatc agggcataga tgcgcttcga
     2641 gttggtgggc gcgatggcga cgtcgatctt gcccaggggc ccgtcgggca agccgtcctt
     2701 ctccaggcgc ttccaggtcg taccgccgtc gtgcgagaga tagatgccgc tgccgggacc
     2761 tccgctgtac tcaccccagg tgtgcatctc cacctgccac atgcctgcga tcagggtgtg
     2821 cggattgtgc gcatcgaggg acaggcccga gcagccgacg ttctcgccgg cgaacaacac
     2881 ccgtgcccaa tgctgcccgc cgtcggtcgt gcggtagacg ccgcgctcct gctgcggtcc
     2941 ggtgacgcgc cccagcacgc aggcgaatac gatatccggg ttcgacggat gcacgacgat
     3001 gcggccgatc cggccggact cggacagtcc catgttcgtc caggtctggc ccgcgtcggt
     3061 cgacttatag ataccgttgc ccatcacgtc gctgtcgcga atcacccacg cctcgccggt
     3121 gccggcccac accgtgccgg gctccgcggg cgcgacagcg agagcgccga ttgccgcaac
     3181 gttctgcttg tcgaagacgg gagcccagcg gttaccgccg tcggatgatt tccacacccc
     3241 gccggaggcc gcaccggcgt agtacacgga cgtgtcaccg ggcactccgg cgactgcggc
     3301 gatgcggttg ccgacgtgcg ggccgacgaa gcggaacttg aaatcgccct ctttgtcggc
     3361 atcggcggcc gccgcgacgc cgactgtcac gctgcccaca gccgcgatcg cgataagcca
     3421 gtggcgtagt gttcgcgtgc gcatcggttg ctgctccgat ccgtgatagt taaggactgt
     3481 tggaatgatt gttcccgcga gtttagcgtg ggacgatgcc gcgatcatta acaagggagt
     3541 ggggaatgaa ccgacgtgta gcgctcgtta caggcggcgg ccggggaata gggcgcgagg
     3601 cggcgctgct gctctcggga gcgggagcgc gtgtgatgac ggtcgcgcgc accgcagccg
     3661 agctcgcgac cgtcgggctc gagtacgtgg cggcggacct cggcacccgt gagggctgtg
     3721 cccttgccgt tgccgagacc gagcagcgac tcggtccggt cgacatactc gtcgtgaacc
     3781 acggcatcgg ctcggcccac gagcggctgg tgtgggagca ggacccggag gtctggcgcg
     3841 agacgatgcg tatcaacctc gacggtccct tcgagctcgc ccgcctcacg gtcggcggca
     3901 tgtgcaagcg cggcttcggc cgtctggtat tcaccagctc gacggcgggc gaaaaggccg
     3961 agcgcgcggg cagtgcctac accgcctcca agcacgggct gatcgggctc gcgcgcgcta
     4021 tcgcccagga cgcgggtccc ttcggggtca catccaacgc agtgctgccc ggctgggtgc
     4081 gcacggcgat ggcggagcgc tcggcgcaga cggaagccga gcgacgcggc gtgagcgtcg
     4141 agcaggtgtg gcgcgagcgc gccgccatct acccgcagaa ccgggtgctg gagccgtgcg
     4201 aggtcgcgca ggtgatcgca ttcctgtgca gcgacgcggc cggcggt
//
LOCUS       DATLKL010000024         4183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_135562, whole genome shotgun sequence.
ACCESSION   DATLKL010000024 DATLKL010000000
VERSION     DATLKL010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135562"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VM713_00295"
     CDS             <1..294
                     /locus_tag="VM713_00295"
                     /inference="COORDINATES: protein motif:HMM:NF025097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HVH82730.1"
                     /translation="GAHAFLGGGSIVRQDVPPYVMVGGNTAVPHAVNSEGLKRRGFTE
                     EQIRAIREAYRILYRSELKLSDALARLEPQAQARPEIRAFVDFIQASTRGIVR"
     gene            291..1484
                     /gene="lpxB"
                     /locus_tag="VM713_00300"
     CDS             291..1484
                     /gene="lpxB"
                     /locus_tag="VM713_00300"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HVH82731.1"
                     /translation="MSALPVRSAAAAPLRVGIVAGEHSGDQLGAALIAALRARTPALE
                     CFGVAGPKMIAAGCEAWAGADSLAVMGLAEVLRHLPRLLRLRAELAARFTAARPDVFV
                     GIDSPEFNLTLARRLKRAGIRCVQYVSPQVWAWRQGRVRTIARDSDLVLCLLPFESDF
                     YAEHGVRAEFVGHPLADQIPLEVDAAGARTALAIAPEARLIALLPGSRLSEVARLAVP
                     FAAAAGWLHARRPELQFIAPMASSAVRECFERQLAQAPEKTLIRVVDGQAQRVLAAAD
                     GAIVASGTATLETLLSGRPLVVAYKVSWLTAFLLRTLGLVKVPYFSQPNLLAGRPLVP
                     ELFQGAVTGEALGRALLAELENPAHLAALRAEFARIHRALRRGGAGLAADAVLQCAAR
                     PPASP"
     gene            1481..2056
                     /locus_tag="VM713_00305"
     CDS             1481..2056
                     /locus_tag="VM713_00305"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HVH82732.1"
                     /translation="MNARARIAGVDEAGRGPLAGPVVAAAVILDPRHRIRGLADSKVL
                     PPEERTRLAGLIRARALAWSIAWADRDEIDALNILEATLLAMRRALLALPVRPTHVQI
                     DGNRCPCLEDLCLGCSFEAIVDGDASVAAISAASILAKTYRDGMMQALDACYPGFELA
                     RHKGYATQAHLKTLRRRRPSPLHRRSFSPMR"
     gene            2060..>4183
                     /gene="dnaE"
                     /locus_tag="VM713_00310"
     CDS             2060..>4183
                     /gene="dnaE"
                     /locus_tag="VM713_00310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HVH82733.1"
                     /translation="MSPGFIHLRLHSEYSLSDSVVRVAELIAAVAAAGMPAVAVTDQN
                     NLFAMVKFYREALEEGVKPIVGVDLAVREDGERREASRLTLLCQSQEGYRNLARLLSR
                     AYLEGQERGVPRVERHWLSPASLAGLIALSGATEGDVGRALVNGREADAERALEQWRA
                     LLPGRYYLELQRVGRQFEETHIAGAVALAARHQLPVVATNDVRFIRAEEFESHEARVC
                     IHDGALLADSARVRRYTRQQYLRSPAEMSALFADLPEALANSVEIARRCSLQLTLGEA
                     RLPQYPVPAGVITEEFLRAEAAGGLARRFPAGAAAPYQERLASELAVICQMGFAGYFL
                     IVADFIRWARDNGVPVGPGRGSGAGSLVAYSLGVTDLDPLEHDLLFERFLNPERVSMP
                     DFDIDFCMEGRDRVIEYVAAKYGRERVSQIITYGTMAAKAVVRDVGRVLGMSYGYVDR
                     IAKLIPFELGITLDAALEKEPELKRLYQAEDEVKNLIDLARSLEGLTRNAGMHAGGVV
                     IAPSVLTDFAPLYCDAAGGSVVTQFDKDDVEAAGLVKFDFLGLRTLTIIDRAVRLINR
                     AAPAGSVPLEIRTLPMDDAATYALLKSCRTTAVFQLESRGMKDLIRRLQPDCFEDIVA
                     LVALFRPGPLQSGMVDDFINRKHAPHEAPIDYLHPRLEPILKPTYGVILYQEQVMQIA
                     QVLASYTLGGADLLRRAMGKKKPEEM"
BASE COUNT          570 a         1520 c         1446 g          647 t
ORIGIN      
        1 ggggcgcacg cttttctcgg cggcggttca atagtgcgcc aggacgtacc accgtacgtg
       61 atggtggggg gcaacaccgc ggtcccgcac gcggtcaaca gcgagggcct gaagcgccgc
      121 ggcttcactg aggagcagat ccgcgccatc cgcgaggcct accggatcct gtatcgctcg
      181 gagctgaagt tgagcgatgc cctcgcgcgc ctcgagccgc aggcccaggc gcggcccgag
      241 atccgcgcct tcgtcgactt catccaggcg tccacccgcg gcatcgtgcg gtgagcgcgc
      301 tgccggtgcg ctccgccgcc gcggcgccgc tgcgcgtcgg gatcgtcgcg ggcgagcact
      361 ccggggacca gctgggagcg gcgctcatcg cggcgctgcg cgcgcgtacg ccggcgctcg
      421 agtgcttcgg cgtcgcgggc ccgaagatga tcgcggccgg ctgcgaggcc tgggcgggcg
      481 ccgacagcct cgccgtcatg ggcctcgccg aggtgctgcg ccacctgccg cggctcctca
      541 ggctgcgcgc cgagctcgcc gcgcgcttca cggcggcgcg ccccgacgta ttcgtcggca
      601 tcgattcccc ggagttcaat ctcacgctgg cgcggcgcct caagcgcgcc ggcatccgtt
      661 gcgtccagta cgtgagcccg caggtatggg cctggcggca ggggcgggtg cgcaccatcg
      721 cccgcgacag cgacctcgtg ctgtgcctgc tgcctttcga gagcgacttc tacgccgagc
      781 acggcgtgcg cgccgagttc gtcggccatc cactcgccga tcagatcccg ctcgaggtgg
      841 acgcggccgg tgcgcgcacg gctctcgcaa tcgcgcccga ggcccgcctg atcgcgctgc
      901 ttcccggcag ccggctctcc gaggtcgccc gcctcgccgt gcccttcgcc gccgcggcgg
      961 gctggctcca cgcgcggcgc cctgagcttc agttcatcgc acccatggca tcaagcgccg
     1021 tacgtgagtg cttcgagagg cagctggcgc aagcgccgga aaaaacactg attcgcgtcg
     1081 tcgacggcca ggcgcagcgg gtgctggcgg ccgcggatgg cgcgatcgtc gcctcgggga
     1141 ccgcgacgct cgagacactg ctctcgggca ggccgctggt ggtggcgtat aaggtgagct
     1201 ggctgacggc gttcctgctg cgtacccttg ggctcgtcaa ggtgccgtac ttctcgcaac
     1261 ccaacctgct cgccggacgg cctctggtcc cggagctctt tcagggcgcc gtcaccggcg
     1321 aggccttagg ccgcgcgctg ctcgccgagc tcgaaaatcc cgcgcacctc gccgcgctgc
     1381 gtgccgagtt cgcgcgcatc caccgtgccc tgcgccgcgg cggcgccggg ctcgccgcgg
     1441 acgccgtgct ccagtgcgcg gcgcgtccgc ccgcctctcc gtgaacgcgc gcgcccgcat
     1501 cgccggggtc gatgaggccg gccgcggacc gctcgccggc cccgtggtgg cggcggcggt
     1561 cattctcgat ccacggcacc gcatccgcgg gctcgccgac tccaaggtcc tgccgccgga
     1621 ggagcgtacg cggctcgcgg ggctgatccg cgcccgcgcg ctcgcctggt cgatcgcgtg
     1681 ggccgaccgt gacgagatcg acgcgctcaa catcctcgag gcgacgctgc tcgccatgcg
     1741 ccgcgcgctg ctggcgctcc cggtgcgccc gacgcacgtc cagatcgacg gcaatcgctg
     1801 cccgtgcctc gaggatctgt gccttgggtg cagcttcgag gcaatcgtcg acggcgatgc
     1861 cagcgtcgct gcgatcagcg ccgcttcgat cctcgccaag acctaccggg acggcatgat
     1921 gcaggcgctc gatgcctgct accccggatt cgagctcgcc cgccacaagg gctatgccac
     1981 gcaggcgcat ctcaagacgc tgcgccggcg ccggccgtcg ccgctgcatc ggcgctcctt
     2041 cagtcccatg cgctaagcga tgtcgcccgg cttcattcac ctccgcctgc acagcgaata
     2101 ctcgctcagc gacagcgtcg tgcgggtagc ggagctgatc gccgccgtgg cggccgccgg
     2161 catgccggcc gtcgccgtca ccgatcagaa caacctgttc gcgatggtga agttctatcg
     2221 cgaggcgctc gaggagggcg tcaagcccat cgtcggcgtc gatctggcgg tgcgcgagga
     2281 cggcgagcga cgcgaggcta gccgcctcac gctgctctgt cagagccagg agggctaccg
     2341 caatctcgcg cggctcctgt cgcgtgccta tctcgaaggc caggagcgcg gcgtgccgcg
     2401 tgtggagcgg cactggctgt cgccggcgag cctcgccggc ctcatcgcgc tctcgggcgc
     2461 caccgagggc gacgtcggcc gtgccctggt gaacgggcgc gaagccgatg ccgagcgcgc
     2521 gctcgagcag tggcgggcgc tcctgcccgg acggtactac ctcgagttgc agcgcgtcgg
     2581 tcgtcagttc gaggagactc acatcgcagg cgccgtggct ctcgcggccc ggcaccagct
     2641 gccggtggtc gctaccaacg acgtgcgctt cattcgggcc gaggagttcg agtcccacga
     2701 ggcgcgcgtt tgcatccacg acggggcgct gctcgcggac tccgcgcgcg tgcgccgcta
     2761 cacgcgccag cagtacctgc gcagtcccgc cgagatgtcg gcgctgttcg cggacctgcc
     2821 cgaggcgctg gcgaactcgg tcgagatcgc gcgccgctgc agcctgcagc tcacgctcgg
     2881 ggaggcgcgg ctgccccaat acccggtgcc ggccggcgtc atcaccgagg agttcctgcg
     2941 cgcagaagcc gccggtggcc tcgcgcggcg cttccccgcg ggcgccgccg cgccctacca
     3001 ggagcggctc gcgagcgagc tggccgtcat ctgccagatg ggtttcgccg ggtactttct
     3061 catcgtcgcg gacttcatcc gctgggcgcg cgacaacggg gtgccggtcg gtcccggacg
     3121 cggctcgggc gcgggctccc tggtcgccta tagcctgggg gtcacggatc tcgatccgct
     3181 cgagcacgac ctgctgttcg agcgcttcct caaccccgag cgcgtgtcga tgccggactt
     3241 cgacatcgac ttctgcatgg aaggccgcga ccgggtgatc gagtacgtcg cggccaagta
     3301 cggccgcgag cgcgtatcgc agatcatcac ctacggcacc atggcggcca aggcggtggt
     3361 acgcgatgtc gggcgcgtgc tcggcatgag ctacggctac gtcgaccgca tcgcgaagct
     3421 catacccttc gagctcggca tcaccctcga tgcggcgctc gagaaggagc cggagctcaa
     3481 gcggctctat caggccgagg acgaggtgaa gaacctcatc gacctggcgc gctcgctcga
     3541 gggcctgacc cgcaacgccg gcatgcacgc cgggggcgtg gtcatcgcac cgtcggtgct
     3601 caccgacttc gcgccgctct actgcgatgc cgccggcggc agcgtcgtca cccagttcga
     3661 caaggacgac gtggaggcgg cgggcctcgt caagttcgac ttcctggggc tgcgcaccct
     3721 cacgatcatc gaccgcgcgg tgcgcctcat caaccgcgca gctcccgccg gcagcgtgcc
     3781 gctcgagatc cgtacgctgc cgatggacga tgcggcgact tatgcgctgc tcaagtcctg
     3841 ccgcacgacc gcggtgttcc agctcgaatc gcgcggcatg aaggacctga tccggcggct
     3901 gcagccggat tgcttcgagg acatcgtggc gctggtcgcg ctcttcaggc ccggcccact
     3961 gcagtcgggc atggtcgacg atttcatcaa ccgcaagcac gcgcctcacg aggcgcccat
     4021 cgactatctg catccgcgcc tcgagccgat cctcaagccg acctatgggg tgatcctcta
     4081 ccaggagcag gtgatgcaga tcgcgcaggt gctggcgagc tacacgctcg gcggcgccga
     4141 cctgctgcgc cgcgcgatgg gcaagaagaa gcccgaagag atg
//
LOCUS       DATLKL010000025         1888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_90506, whole genome shotgun sequence.
ACCESSION   DATLKL010000025 DATLKL010000000
VERSION     DATLKL010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90506"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..442)
                     /locus_tag="VM713_00315"
     CDS             complement(98..442)
                     /locus_tag="VM713_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HVH82734.1"
                     /translation="MQGSFLRFYVHEGQRYRGRPVWEWLLEEAKGFGIRGGSAFHAMA
                     GFGRHHVLHESRFFELAGRLTVEVEFFVTDDEAGKFLAHLEPHAVRLFYARIPASFGI
                     LHPDGADPQPAA"
     gene            complement(446..826)
                     /gene="crcB"
                     /locus_tag="VM713_00320"
     CDS             complement(446..826)
                     /gene="crcB"
                     /locus_tag="VM713_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016139391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HVH82735.1"
                     /translation="MWRSIAAVSVGSSLGALLRWWLGARLNAHFPPIPPGTLAANLIG
                     GYVVGVAVAFFATYTAVAPEWRLLVITGFCGGLTTFSTFSAELVTLLQQGRATWALAA
                     AAAHLGGSLLMTLAGIGTVVWARS"
     gene            918..>1888
                     /locus_tag="VM713_00325"
     CDS             918..>1888
                     /locus_tag="VM713_00325"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HVH82736.1"
                     /translation="MSTFRPGAAALLLLALPAALTGGCSPEARQHAADQRLRAIYEAE
                     WRWRKEQLPGGEDSTRPLADHLPRVDPTSQEQRRARWEETLAKVNALAREDLSPREQV
                     NYDVYRAQLQVLIDHQRFRDFEMPANSDSSFWTDLGYTARRPFRTLTDYEHWIAQMRD
                     IPRYFAEQMDEMRAGMKRGFTPPRVTLAGRDASITAVTDATPEGSLFYTPFGEMPGVA
                     PERQVALRAEAIATIRDVVQPAYRTLLAFVRTEYLPGTRTTLAAEALPDGKRYYRAKI
                     REFTTLDMDPDAIHNLGLTEVQRLHEAMGAAMHETGFRGDFAAFLNFL"
BASE COUNT          315 a          690 c          633 g          250 t
ORIGIN      
        1 gaagtagccc cgctcgccgc gcccgagccg ggccgcccgg cgcgcgcgcc ttcggcgacc
       61 gtgccgctcc atagcatcga gcggaagtga cgcggcctca cgcggcgggc tgcggatcgg
      121 cgccgtcggg atggagaatg ccgaaactcg cggggatgcg cgcgtagaac agccgcaccg
      181 cgtgcggctc gagatgcgcg aggaacttac cggcctcatc gtccgtgacg aagaactcga
      241 cttccacggt gagcctgccg gcgagctcga agaagcgact ctcgtgcagc acgtggtggc
      301 ggccgaagcc cgccatggcg tggaaagccg agccaccgcg gatcccgaag cccttggcct
      361 cctcgagcag ccattcccac accggccgcc cgcgatagcg ctggccctcg tgcacgtaga
      421 agcggagaaa cgagccttgc accgctcagc tccgcgccca gacgactgtg ccgatgccgg
      481 cgagtgtcat gaggagcgac ccgccgagat gcgccgcggc ggcggcgagc gcccaggtcg
      541 cgcgcccctg ctgcagcagc gtcacgagct cggccgagaa ggttgagaag gtggtgagcc
      601 cgccgcagaa gcccgtgatc accagcaagc gccactcggg cgcaaccgcg gtgtaggtcg
      661 cgaagaacgc caccgccacg ccgaccacgt aaccgccgat caggtttgcc gcgagcgtgc
      721 ccggcggaat gggcgggaag tgcgcgttca agcgggcgcc gagccaccag cgcagcagcg
      781 ccccgaggct cgatccgaca ctgacggccg caatggatct ccacatggtg tcagcccctt
      841 gtacctccga gcccggggcg cgtgcaacgc ttgcagtatg ctgtcgcacc ctctagaggg
      901 gccaagaaag gaagcccatg tccaccttcc ggcccggcgc cgcggcgctg ctgctgctcg
      961 cgctgcccgc cgcgctcacg ggaggctgct cgcccgaggc acgccagcac gccgcggacc
     1021 agcgcttgcg cgccatctac gaggccgagt ggcgctggcg caaggagcag ctcccgggcg
     1081 gcgaggacag cacccggccg ctcgccgacc acctgccgag ggtcgatccg acctcgcagg
     1141 agcagcggcg cgcgcgctgg gaggagaccc tcgccaaggt gaatgcgctc gcgcgcgagg
     1201 atctctcccc ccgcgagcag gtgaactacg acgtctaccg cgcgcagctt caggtgctga
     1261 tcgaccatca gcgcttccgt gacttcgaga tgccggccaa ctccgactcg agcttctgga
     1321 ccgatctcgg ctataccgcg cggcggccgt ttcggacgct cacggactac gagcactgga
     1381 tcgcgcagat gcgcgacatc ccccgctact ttgccgagca gatggacgag atgcgcgccg
     1441 gcatgaagcg cggcttcacg cccccgcgcg tcacgctcgc cgggcgcgat gcctcgatca
     1501 ccgcggtcac ggacgcgacc cccgagggca gcctcttcta tacgcccttc ggggagatgc
     1561 cgggtgtcgc cccggagagg caggtggcgc tgcgcgccga ggccatcgcg accatccgcg
     1621 acgtggtaca gccggcgtac cgcacgctcc tcgcttttgt gcgcaccgag tacctgccgg
     1681 gcacccgcac cacgcttgcg gcggaggcgc tccctgacgg caagcgctac taccgcgcca
     1741 agatccgcga gttcaccacg ctcgacatgg acccggatgc gatccacaac ctcgggctca
     1801 ccgaggtgca gcgcctgcac gaggcgatgg gcgctgccat gcacgagacc ggtttccggg
     1861 gcgactttgc cgcgttcctc aactttct
//
LOCUS       DATLKL010000026         1920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_34175, whole genome shotgun sequence.
ACCESSION   DATLKL010000026 DATLKL010000000
VERSION     DATLKL010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34175"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..695)
                     /locus_tag="VM713_00330"
     CDS             complement(195..695)
                     /locus_tag="VM713_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82737.1"
                     /translation="MLLALTVWAVSWAGPDAQTPADPFERLKALAGEWRADLPGYGQI
                     LDSSRLVSKGTAVEETLGTPSDNEVSLYTRDGERLLLTHFCALTGDGHVARLATPALR
                     GSPARLQFTLVSVSNLHDKTAPHMRQVLITVVDRDHIIEKWTKTEKGVDTVFDLRFSR
                     RAPQPP"
     gene            849..1637
                     /locus_tag="VM713_00335"
     CDS             849..1637
                     /locus_tag="VM713_00335"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVH82738.1"
                     /translation="MQRSALVLILLSLGSANGAVREHEVDYTSHKVVLKGSLAFDDAL
                     SGLRPAVLVVHEWSGLNEHARHNARRLAEAGYVALALDMYGGAIQAHRPKEALLLSTE
                     IRTNLPLMQARFDAARDLLRTQPHVDPTRIAAIGYCFGGGVVLDMARAGEDLRGVVSV
                     HGVLDTAAPAKAGALKAEVLVLTGGADPYVTRDQVDALDRELKSAGARYRIVTYPEAK
                     HSFTNPAAKEYGGKQFDMPGDEYNAAADQQSWLEILTFLRRVMK"
     gene            complement(1672..>1920)
                     /locus_tag="VM713_00340"
     CDS             complement(1672..>1920)
                     /locus_tag="VM713_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82739.1"
                     /translation="NETSVVRLHSGNGPHLEMDQRAIACFDKALNDVGLARNEQLRGV
                     LHDYFAWATTTTMARFHDSADDVPDGLLIPQWSWHGLL"
BASE COUNT          338 a          610 c          646 g          326 t
ORIGIN      
        1 agaggcagcg ggcttcagcg gcggcgtcag tgccggtggt ggggtaacaa tgatgtcgcg
       61 cggttccgtc acccccgcgc ggatcgcctt cggcctggca tcgatactcc ttgggctggc
      121 gtcattgtgg gcgtggaagc aggtctggcg cggacgcggg tgagcgccgc gtgacattca
      181 cacggccggg atgctcacgg cggctgcggc gcccggcggg agaagcgcag gtcaaacacg
      241 gtatccacgc ccttctccgt cttggtccat ttctcgatga tatggtcccg gtcaaccacc
      301 gtaatcagca cctgcctcat gtggggcgcg gttttatcgt gcaggttgct caccgagacc
      361 agagtgaact gcaggcgggc gggcgatccg cgcagggcgg gagtcgcgag ccgcgccacg
      421 tggccgtcgc ccgtcagcgc acagaagtgc gtcaggagca gccgctcacc gtcgcgggtg
      481 tagagcgaga cctcgttgtc gctcggcgtg ccaagggtct cctcgacggc cgtgcccttc
      541 gacaccagtc ggctcgagtc gagaatctgg ccgtagcccg ggagatcggc ccgccattcg
      601 ccggcgagag ctttcaggcg ctcgaaggga tcggcgggcg tctgcgcgtc cggtccggcc
      661 cagctcacgg cccacaccgt cagtgcgagc aacactcgaa ttcgcacgcg ctgcgacctc
      721 attgcggctg aaaggaagtc ggtgggcggc attatgcgcg cccgcgtgac cacgaccaag
      781 gatggccggg catacacggc gtccggaaga gcggatgatg tcgatcagga cgaggtatga
      841 gagtctccat gcagcgcagc gccctggtgt tgattctgtt gtcgcttggc agcgcgaacg
      901 gcgccgtcag ggagcatgag gtcgactaca catcgcacaa ggtcgtactg aagggctccc
      961 tcgcctttga cgacgccctc tctggactgc gccctgcggt cctcgtggtt cacgagtggt
     1021 cggggctcaa tgagcacgcg cgtcacaacg cccggcggct cgccgaggcg ggttacgtcg
     1081 cgctcgccct cgacatgtat ggcggcgcca ttcaggcgca ccgtcccaag gaagccctcc
     1141 tgctctcgac ggagatccgg acgaacctgc cgctgatgca agcgcgcttt gatgccgcgc
     1201 gcgatctgct ccgcacgcag ccgcatgtag atccgacgcg catcgctgcg atcggctact
     1261 gcttcggtgg gggcgtggtg ctcgacatgg cgcgcgccgg ggaggacttg cgcggcgtcg
     1321 tgagtgttca cggcgtgctc gacaccgccg cccctgcaaa ggccggcgcg ctgaaggctg
     1381 aggtgctcgt gctgaccggc ggcgccgatc cctacgtgac aagggatcag gtggacgcgc
     1441 tcgatcgcga gctcaaatcc gccggagcgc gctataggat cgtaacctat cccgaggcga
     1501 agcattcctt caccaacccg gcggcgaagg agtacggcgg caagcaattc gatatgccgg
     1561 gagatgagta caacgccgct gccgaccagc agtcctggct cgagatactg acgttcctca
     1621 ggagggtgat gaagtgagac gccgagagca tgcaggcgtg acgcgcaatc gctaaaggag
     1681 accgtgccag gaccactgcg gaatcagcaa gccatcgggc acatcgtccg cggagtcgtg
     1741 aaaccgagcc atcgtagtcg tggtggccca cgcgaagtag tcgtgcagca ctcctcgcag
     1801 ctgctcattg cgcgccagcc cgacatcatt caacgctttg tcaaaacacg cgatcgcccg
     1861 ctgatccatc tccaggtgcg gaccgttgcc gctgtgaagc cggaccacag aagtctcatt
//
LOCUS       DATLKL010000027         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_56685, whole genome shotgun sequence.
ACCESSION   DATLKL010000027 DATLKL010000000
VERSION     DATLKL010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56685"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VM713_00345"
     CDS             complement(<1..333)
                     /locus_tag="VM713_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HVH82740.1"
                     /translation="MHVHILGIGGTFMGGVAAIARAAGWRVTGSDRDMYPPMSTQLEA
                     LGIEVTAGFGAEQLDLKPDVVVVGNVMTRGMPVVEALLEAGKPYLSGPEWLAREVLKD
                     RWVLAVAGT"
     gene            complement(345..860)
                     /locus_tag="VM713_00350"
     CDS             complement(345..860)
                     /locus_tag="VM713_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82741.1"
                     /translation="MREVIGLYVQIALLRRGPQDLPASRLLLILTVCAYAAVNALVSS
                     LLPPSTGWPLALAIEVLFQLAWYAALLELTSRRERFLQTTTAVFGFQSVLAPLLIASE
                     WLMRRFGQDSTWQLPIAVGGLALVIWVIAANGQVVKAALDWSSATSVALVILQIVATQ
                     LLMLALFPQSG"
     gene            925..2013
                     /gene="rnd"
                     /locus_tag="VM713_00355"
     CDS             925..2013
                     /gene="rnd"
                     /locus_tag="VM713_00355"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HVH82742.1"
                     /translation="MRLQTTVTTAAALAEICARLGAEERIGLDTEFLRERTYSARLCL
                     VQLAAPDEAACVDPLALAELAPLAALLAAPGTLKVMHASRQDLEVLYPLAGLTRPVFD
                     TQIAAALAGFPAQVGYAELARRLLGQELAKSHTRTDWSRRPLSPAQIEYALDDVRYLL
                     PIAAELENRLARLGRLAWLTEELAGLEETRGFVTAPESAWQRVRGLRGLDPGRERLAR
                     SLAAWRERRALEHDRPRGWILDDGPLREIVLRVPRSAAQLAAIPELPAGVVKHSGAEL
                     LACVAAADIPDPPPPVRAKPQSDPQRNALVRKLGGISQAVAAELELVPEVLATRRELE
                     LLADGRRDGAVLRGWRRSVLGERLLAAL"
BASE COUNT          325 a          818 c          730 g          302 t
ORIGIN      
        1 ggtgccggcc accgccagca cccagcggtc cttcagcacc tcacgcgcga gccattccgg
       61 tcccgaaagg tacggcttac ccgcctcgag cagcgcttcc acgaccggca tgccgcgggt
      121 catcacgttg ccgaccacca ccacgtctgg cttcagatcg agctgctcgg cgccgaatcc
      181 cgcagtcacc tcgatgccga gcgcctcgag ctgcgtgctc atcggcgggt acatgtcgcg
      241 gtcggaaccg gtgacgcgcc agcccgccgc acgcgcaatc gccgcgacgc cgcccatgaa
      301 agtaccgccg atgccgagta tgtggacgtg cactttggcg cccgtcagcc gctctgggga
      361 aacagcgcga gcatcagcag ctgcgtcgcg acgatctgca gaatcaccag cgccacgctc
      421 gtggcgctcg accagtcgag cgccgccttc accacctggc cgttggcggc gatcacccag
      481 atcaccagcg cgagtccccc gaccgcgatc gggagctgcc aggtcgagtc ctgcccgaag
      541 cgccgcatca gccactcgga ggcgatcagc agcggcgcca gcaccgactg gaagccgaac
      601 accgcggtgg tggtctgcag gaagcgctcg cggcggctgg tgagctcgag cagcgccgca
      661 taccaggcga gttggaacag cacctcgatg gccagcgcca gaggccagcc cgtgctcggc
      721 ggcagcaggc tgctgaccag cgcgttcacc gccgcgtagg cgcacacggt gagaatcagc
      781 agcagccgcg aggccggcag gtcctgcggc ccgcggcgca gcagcgcgat ctgcacgtag
      841 agaccaatga cctcccgcat cactttcggt accgccaaag cctgcgcata attgcgcccg
      901 ttgcgattgt acagatcatc gccattgcgt ttgcagacga ccgtcacgac agcagcggct
      961 ctggccgaga tctgcgcgcg cctcggcgcc gaggagcgca tcggcctcga caccgagttc
     1021 ctgcgcgagc gcacctactc cgcgcggctc tgcctggtac agctggcggc gccggacgag
     1081 gccgcctgcg tcgatccgct cgcgctcgcg gagctcgcgc cgctcgcggc gctgctcgcc
     1141 gccccgggca cactcaaggt catgcatgcc tcgcgccagg acctggaagt gttgtatccg
     1201 ctcgcgggcc tgacgcgccc cgtgttcgac acgcagatcg ccgcggcgct cgccggcttt
     1261 cccgcgcagg tcggctacgc cgagctcgcg cggcggctgc tcggccaaga gctcgcgaag
     1321 tcgcataccc gtaccgactg gtcgcggcga ccgctgtcgc cggcgcagat cgaatatgcc
     1381 ctcgacgatg tgcgctacct gctgccgatc gccgccgagc tcgagaaccg gctcgcgcgt
     1441 ctcgggcggc tcgcttggct caccgaggag ctggccggcc tcgaggagac gcgcggcttc
     1501 gtcaccgcgc ctgaatcggc ctggcagcgc gtgcgcggac tgcgcggcct cgatccgggc
     1561 cgcgagcgcc tggcgcgcag cctggccgcc tggcgcgagc ggcgcgcgct cgagcacgac
     1621 cgcccacgcg gctggattct cgatgacggc ccgttgcgcg agatcgtact gcgcgtgccg
     1681 cgcagcgcgg cccagctcgc cgcgatcccc gaacttccgg ccggggtggt gaagcacagc
     1741 ggcgcggagt tgctcgcctg cgtcgcggcc gccgacatcc ccgacccgcc accgcccgtg
     1801 cgggccaagc cgcagtccga cccgcagaga aacgcgctcg tcagaaagct cggcggaatc
     1861 agtcaggcgg tcgccgccga gctcgaactg gtcccggagg tgctggcgac gcggcgcgag
     1921 ctcgagctgc tcgccgacgg ccggcgcgat ggcgcggtgc tccgtggctg gcgccgcagc
     1981 gtcctcggcg agcgcctgct cgccgcgctc tagtctgaac tgctcgggat cgccacgtcc
     2041 ctgtgccgcc cctcgcgcgg cgagccaaaa gcgcggtttc ggctcgccgc tcgccgcctg
     2101 cggcggcggg cacatccatg tgcctgcagc gaattgactc ctaacggtct ggcacgcgcg
     2161 cgaaggacag ccatg
//
LOCUS       DATLKL010000028         1588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_113082, whole genome shotgun sequence.
ACCESSION   DATLKL010000028 DATLKL010000000
VERSION     DATLKL010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113082"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1588)
                     /locus_tag="VM713_00360"
     CDS             complement(<1..>1588)
                     /locus_tag="VM713_00360"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HVH82743.1"
                     /translation="PGYDYQPDWSPDGRSIVYASSRGAAIELMLLDLETGRSAQLTHG
                     GAVNVEPRFSPDGHRIVFVSSAYQRHLHVFLAEVRGQELAEPVRLTGENRSALPRYYY
                     SAYDHEINPVWTRDGTAIVYVSNRGHIYGTGGFWRMPVSAGAEATELHYEETSWQARP
                     DFSPDGQRLVYSSYLGRNTLQLWLLPAVAGGDPVALSYGDFDETGARWSPDGQQIAYI
                     SNRGGGLELRLLRFPGGSSRALEISERRRLRPGGLLHLTVRDDHDAATPARVVVTDAA
                     GRFYGPSGAWLHHAEFDRNEYPFEARYFHTAGDELIEVPAGPVSVEVMKGLARTPVRR
                     SVTVNPGATSELAVVLPARRWPDGAGRHWVSADVHVHVNYGGHYRNTPAHLVLQAEAE
                     DLDIVENLVVNKEQRYPDIAYSGRGVDPASTPATLVVHGQEFHTSYWGHLGLLGGQGQ
                     TLLPGYAGYPNTAAASLYPMNADVADLAHAQGALVGYVHPFEEDPRPLTHPAHTDADE
                     LPVDVALGKVDYLEIVSFAD"
BASE COUNT          253 a          530 c          554 g          251 t
ORIGIN      
        1 tgatcggcga aggagacgat ctccaggtaa tcgaccttgc cgagcgcgac atcgacgggc
       61 agctcatccg catcggtgtg agccggatgg gtgagcggcc gcgggtcctc ttcgaaagga
      121 tgcacgtagc caaccagcgc gccctgggcg tgggcgagat ccgccacatc ggcgttcatc
      181 gggtagaggc tcgccgccgc ggtgttggga tagccggcat agcctggcaa cagcgtctgg
      241 ccctgtccgc ccagcaggcc caggtgcccc cagtagctgg tgtgaaactc ctgtccgtgc
      301 accaccagcg tcgcgggcgt cgaggcggga tcgacaccgc ggccgctgta ggcgatgtcg
      361 gggtagcgct gctccttgtt gacaaccagg ttctcgacga tgtcgagatc ttcggcctcg
      421 gcctgcagca ccaggtgtgc gggcgtgttg cggtaatggc cgccgtagtt cacgtgcacg
      481 tgcacgtcgg cgctcaccca atgtcggccc gccccgtcgg gccagcggcg tgccggcagc
      541 accacggcga gctcgctcgt cgcgccgggg ttcacggtga cgctgcgccg taccggcgtg
      601 cgcgccagcc ccttcatcac ctcgacgctc acggggccgg ccggcacctc gatgagctcg
      661 tctcccgcgg tgtggaagta gcgcgcctcg aagggatact cgttgcggtc gaactcggcg
      721 tggtgcagcc acgcgcctga tggtccgtag aaccgccccg cagcgtcggt cacgacgacg
      781 cgcgccggcg tcgcggcgtc gtggtcgtcg cgcaccgtga gatgcagcag tccgcccggg
      841 cgcagacggc gtcgctcgct gatctcgagt gcccgcgagc tgccgccggg gaagcgcagc
      901 agacgcagct cgagcccccc gccgcgattg gagatgtagg cgatctgctg cccgtcgggt
      961 gaccagcgtg cgccggtctc atcgaagtcc ccatagctga gcgcaacagg gtcgccgcca
     1021 gcgacggccg gcagcagcca cagctgcagc gtgttgcgcc caagatagga gctgtagacg
     1081 aggcgctgtc cgtcgggcga gaaatccgga cgcgcctgcc agctggtctc ctcgtaatgc
     1141 agctcggtag cctcggcgcc cgcactcacc ggcatccgcc aaaaaccgcc ggtgccgtag
     1201 atgtggccgc gattcgagac atagacgatc gcggtcccat cgcgcgtcca caccggattg
     1261 atctcgtgat cgtaagcact gtagtagtaa cgcggcagcg cgctgcggtt ttcacccgtg
     1321 aggcgcacgg gctcggcgag ctcctggccg cgcacttcgg cgaggaacac atgcagatgc
     1381 cgctgatagg cgctcgacac gaatacgatg cgatgtccat cgggcgagaa gcgcggctcg
     1441 acgttgaccg cgccgccgtg ggtgagctgc gcgctgcggc cagtctcgag atcgagcagc
     1501 atgagctcga tcgctgcgcc acggctcgag gcgtagacga tcgagcggcc gtcgggcgac
     1561 cagtcgggct gatagtcgta gccgggac
//
LOCUS       DATLKL010000029         2677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_214454, whole genome shotgun sequence.
ACCESSION   DATLKL010000029 DATLKL010000000
VERSION     DATLKL010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214454"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..>2677
                     /locus_tag="VM713_00365"
     CDS             107..>2677
                     /locus_tag="VM713_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVH82744.1"
                     /translation="MAIPPPPDKPQDFTASDILSADAWTGRHTRPALGIDVACTKIAV
                     DFQNMRRDEVDACVRRNLELLQGATGCDCVFLAALQSDAPVIQDVQVARSAFAQCKPE
                     GLTGLSLDALPWLKGRLEHLRLSELRDTSSPRKEQLAEAAKLADLQIGSALFISFRVQ
                     DKPAGLLALAYTLPRGAWDVNLQLLMKLIGTSLATGLERIRTEARLAKLEERTNLFQA
                     AANDGLWDFDVESNEVFFSPRWKAMLGYADEDMHGSPDWRSLVHPDDLARVQTAIRDH
                     VAGKTPIFESTHRMRHRNGEWRWVISRATARVDKQGRLLRLVGVELDITERKLYEEAL
                     FREKESAQITLQSIGDGVITTDANSTIDYINPVAEELTGWRLEDAMGRPVEEIFRAFH
                     EETCEPLENPLTVSIRRIRPIKSVRPMLLIRRDGNELYVESTAAPIRDGQGHVAGGVL
                     VFHDVSESRELNRRLSYHASHDLLTGLVNRREFESRLERALKSAKAREASYALCHLDI
                     DQFKIVNDTCGHGAGDALLGQVGALLKSKIRWRDTLSRLGGDEFGILLESCSLDEAMR
                     TAETLREAVRNFRFTWEDRVFRLGASIGVVPITAENEDVASVLSAAGSACTAAKEGGR
                     NRVHSFAENDIELMRRRREMQWAARINAALEEGRFELYRMAIQPLQRVEPGAHYELLL
                     RLKDETGRIVAPNDFIAAAERYGITPAIDRWVIENAFRWLVSEADERQNLVQCSINLS
                     GQSLGDDKFLPFVIDQLRHSGLDASKICFEITETAAVASFSQANRFIQALKQQGCKFA
                     LDDFGTGLSSFGYLKHFPVDYLKIDGSFVREILRDPIDREMVRSINEIGHLTGKQTIA
                     E"
BASE COUNT          476 a          921 c          867 g          413 t
ORIGIN      
        1 ggaaagcggc actgaacaat cacgtgcttc cgtgacgggg tatcaagttc tcgacgtaac
       61 atttgaatta agctgcgccg gttcggtaat gggaggtcac ggataaatgg cgattccgcc
      121 cccgccggat aagccacagg acttcaccgc gtccgacatt ctttcggccg acgcctggac
      181 gggccggcac accaggccgg ctctgggaat cgacgtcgcc tgcacgaaga ttgcggtcga
      241 tttccagaac atgcgccgtg acgaggtcga cgcctgcgtg cggcgcaacc tcgagctgct
      301 gcaaggggcc accggctgcg actgcgtgtt cctcgcggcg ctccagagcg acgcgcccgt
      361 aatccaggac gtgcaggtcg cccgcagcgc cttcgcacag tgcaagcccg aagggctaac
      421 gggcctctcc ctcgacgcct tgccctggct gaagggccgg ctcgagcacc tgcgcctatc
      481 ggagctgcgc gacacgagct ccccacgcaa ggagcagctg gcggaagcag ccaagctcgc
      541 ggatctgcag atcggctcgg cgctcttcat cagctttcgg gtgcaggaca agcccgcagg
      601 tctcctcgct ctcgcgtaca cgctcccgcg cggggcgtgg gacgtgaacc tgcagctgct
      661 catgaagctc atcggcacca gcctcgccac cggcctcgag cgcatccgca ccgaggcgcg
      721 gctcgccaag ctcgaggagc gcaccaatct cttccaggcc gccgccaacg acggcctgtg
      781 ggacttcgac gtcgagagca acgaagtatt cttctcgccg cgctggaagg cgatgctcgg
      841 ctacgcagac gaggacatgc acggctcgcc cgactggcgc agcctcgtgc acccggacga
      901 cctggcgcgg gtgcagactg ccatccgcga tcacgttgcg ggcaagaccc cgatcttcga
      961 gagcacccac cgcatgcgcc accggaacgg cgagtggcgc tgggtcatca gccgcgccac
     1021 ggcgcgcgtc gacaagcaag ggcggctgct gcgcctggtg ggcgtcgagc tcgacatcac
     1081 cgagaggaag ctctacgagg aagcgctgtt tcgcgagaag gagagcgcgc agatcacgct
     1141 ccagtcgatc ggcgacggcg tcatcaccac cgatgccaat tcgaccatcg actacatcaa
     1201 cccggtggcc gaggagctca ccggctggcg actcgaggac gcgatgggca ggcccgtgga
     1261 ggagatcttc cgcgccttcc acgaggagac ctgcgagccg ctcgagaacc cgctcaccgt
     1321 ctcgatccgc cgcatccgcc cgatcaagtc ggtgcggccg atgctgctca tccgccgtga
     1381 tggcaacgag ctctacgtcg agagcacggc ggcgccgatc cgcgacggcc aggggcatgt
     1441 cgccggcggc gtgctggtgt tccacgacgt cagcgagtcg cgcgagctca accggcgcct
     1501 ctcctaccac gcgagccatg acctcctcac cgggctggtg aaccgccgcg agttcgagag
     1561 ccgcctcgag cgggcgctga agagcgccaa ggcgcgcgag gcctcctacg cgctctgcca
     1621 cctcgacatc gaccagttca agatcgtcaa cgacacctgc ggacacggcg cgggggacgc
     1681 gctcctcggg caggtgggtg ccttgctcaa gtccaagata cgctggcgcg acacgctctc
     1741 gcgtctcggc ggcgacgagt tcggcatcct tctcgagagc tgctcactcg atgaggcgat
     1801 gcgcaccgcg gagacgctgc gcgaggcggt gcgcaacttc cgcttcacct gggaggatcg
     1861 cgtgttccgg ctcggcgcct cgatcggcgt cgtgccgatc acggccgaga acgaggacgt
     1921 cgcctcggtc ctttccgccg ccggcagtgc ctgcaccgcg gccaaggagg gcgggcgcaa
     1981 ccgcgttcac agctttgccg agaacgacat cgagctgatg cgacgccggc gcgagatgca
     2041 gtgggcggcg cgcatcaacg cggcgctgga ggagggccgc ttcgagctgt atcgcatggc
     2101 gattcagccg ctgcagcgcg tcgagcccgg cgcgcactac gagctgctgc tgcgcctcaa
     2161 ggacgagacc gggcgcatcg tggcgccgaa cgacttcatc gcggccgccg agcgctacgg
     2221 catcacgccg gcgatcgacc gctgggtgat cgagaacgcc ttccgctggc tggtgtcgga
     2281 ggccgacgag cggcagaacc tcgtgcagtg ctcgatcaat ctctccggcc agagcctcgg
     2341 cgacgacaaa ttcctgccgt tcgtcatcga tcagctgcgc cacagcgggc tcgatgcctc
     2401 gaagatctgc ttcgagatca ccgagaccgc ggcggtggcg agcttctccc aggccaaccg
     2461 cttcattcag gcgctcaagc agcagggctg caagttcgcg ctcgatgatt tcggcaccgg
     2521 gctttcctcg ttcggctacc tcaagcactt cccggtcgat tacctgaaga tcgacggcag
     2581 cttcgtgcgg gaaatcctcc gcgatcccat cgaccgcgag atggtgcgct cgatcaacga
     2641 gatcggacac ctcaccggca agcagaccat cgccgag
//
LOCUS       DATLKL010000030         1745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_135676, whole genome shotgun sequence.
ACCESSION   DATLKL010000030 DATLKL010000000
VERSION     DATLKL010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135676"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VM713_00370"
     CDS             complement(<1..212)
                     /locus_tag="VM713_00370"
                     /inference="COORDINATES: protein motif:HMM:NF044430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82745.1"
                     /translation="MSDIETALAQGLEESFPAHLAALCERATRSLTACGFTGLLVHSG
                     SALTIFEDDRTYPFVAHAPFKVWVPLS"
     gene            753..1280
                     /locus_tag="VM713_00375"
     CDS             753..1280
                     /locus_tag="VM713_00375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVH82746.1"
                     /translation="MTAVTVSVRDARAQPADRLWIQSVYRDYLDDLNPGTGLFPKLGE
                     IGHREPDQIAHWFADPNTFPLVILRGSEPVGFARVLRASPAPPPRPRSDYRMAEFFIT
                     RARRRLGVGRTAVELILNRFAGRWEIIEYLRNADAVRFWRRVVGGYTRGNYQERIVNG
                     EVHQVFESGAPPRPA"
     gene            complement(1225..1500)
                     /locus_tag="VM713_00380"
     CDS             complement(1225..1500)
                     /locus_tag="VM713_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82747.1"
                     /translation="MSPLLQGVLVGLIVAACAIYSAWRLASLKLRLRVLDGLASLPPQ
                     LTAAWLGALRQRTLAQLAGGCAGCAAGGVATPDAAARRTQTPGALRR"
     gene            complement(1514..>1745)
                     /locus_tag="VM713_00385"
     CDS             complement(1514..>1745)
                     /locus_tag="VM713_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82748.1"
                     /translation="LAPIISHTWSLATGLSLLAWYVFAPQCLSTLAVVKRETNSWTYP
                     LAMAAYLFVLAYLAAFLTYRVALALGGGAVLG"
BASE COUNT          303 a          607 c          565 g          270 t
ORIGIN      
        1 gagagcggga cccacacctt gaagggggca tgcgctacga acggataggt tcgatcgtct
       61 tcgaaaatgg tgagcgctga gcccgaatgc accagcaacc ccgtgaagcc acaggcggtc
      121 agcgaccggg tggcgcgctc gcagagcgca gccaggtgcg cagggaagga ttcctccagc
      181 ccctgcgcca aagcggtctc aatgtcggac attcacttgt ctcccgtcgg ccacaaccgc
      241 tgccggcctg tcgcaccggc ccgacagcac tttccaatcg taccggtact tctatagaat
      301 ttaccttctc cgcggctcga atatgagccc ggaacggcct cacccggctg tggtctatca
      361 acccaactct tgcttcctcg gggaactatc gatgaacact aaacttgctc ttagcgcaat
      421 cgttgcggcg ctgatgctga cggcctgcgc gaagcaggaa gccgcaggca ccgacaccgc
      481 tgctactcct cctgctccgg ctgccgcccc ggctgcagct cccgctcccg ctgatcaggc
      541 tgccgcccca gccgccggca cggctgctcc tgctgacgcc ggcgctgctg ccccggctgc
      601 gggtgcgatg cctcaggctg aaggcaccgc cgctccgaag ccggctgagc agccaaagaa
      661 gtaagtcctt aagggcttac ggctgaaaat gcggcccgga cggatcgcca gcgcgagccg
      721 tccgggccgt gtcattctcg ggtcctgacg cgatgaccgc cgtgactgtc agcgtgcgcg
      781 atgcacgcgc ccagcctgcc gaccggctct ggatccagag cgtctatcgc gactatctcg
      841 atgacctgaa tcccggcacc gggctgttcc ccaagctcgg ggagatcggg caccgtgagc
      901 ccgaccagat cgcgcattgg ttcgccgatc ccaatacctt tccgctggtg atcctgaggg
      961 gcagtgagcc ggtaggcttc gcgcgcgtgc tgcgcgcaag tcccgcacca ccgccgcggc
     1021 cgcggagcga ctaccgcatg gccgagttct tcatcacccg cgcgcggcgc cgtctcggcg
     1081 tcgggcgcac cgcggtcgag ctgatcctca atcgcttcgc tgggcgctgg gagatcatcg
     1141 agtacctgcg caatgcggat gcggtgcgct tctggcggcg ggtggtcggc ggctatacgc
     1201 gcggtaacta tcaggaacgc atcgtcaacg gcgaagtgca ccaggtgttt gagtccggcg
     1261 cgccgccgcg tccggcgtag ctacaccccc cgcggcgcag ccggcacagc cgccggcgag
     1321 ctgcgcgagc gtgcgctgcc gcagcgcgcc gagccacgct gcggtgagct gtggcggcaa
     1381 tgaggcgagc ccatcgagca cccgcagtcg cagcttgagc gaggcgagcc gccaggcgct
     1441 gtagatcgcg caggcggcga cgatcaggcc gacgagcacg ccttgcagca gcggactcat
     1501 gcggaccgcc ctctcatccg agcaccgcgc cgccgccgag tgccagcgcc acgcgatagg
     1561 tcaggaaggc cgcgaggtac gcgagcacga acagatacgc cgccatcgcg agcggatagg
     1621 tccaggagtt ggtctcgcgc ttcaccaccg cgagtgtcga caggcactgc ggcgcgaata
     1681 cgtaccacgc cagcagcgac agcccggtgg caagactcca ggtgtgactg atgatcggcg
     1741 ccagg
//
LOCUS       DATLKL010000031         2988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_56742, whole genome shotgun sequence.
ACCESSION   DATLKL010000031 DATLKL010000000
VERSION     DATLKL010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56742"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /gene="ccmE"
                     /locus_tag="VM713_00390"
     CDS             complement(<1..445)
                     /gene="ccmE"
                     /locus_tag="VM713_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003117796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HVH82749.1"
                     /translation="MTPRQRRMTLVLTIVAGVAVAGALALSAFRQNVTFFFDPSQVTA
                     GKVPAGERFRLGGMVTKGSLRRDPGSLEVHFVVTDFQSNVPVSYTGVLPDLFREGAGV
                     VTHGRMQDGTFVADEVLAKHDEKYMPPAVAESLKRHGESREGLVTP"
     gene            complement(442..603)
                     /gene="ccmD"
                     /locus_tag="VM713_00395"
     CDS             complement(442..603)
                     /gene="ccmD"
                     /locus_tag="VM713_00395"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HVH82750.1"
                     /translation="MKEFLDMGGYAAFVWPAYAVTLAVAVLNVVWARRSLARATAAAR
                     RRLAAGGSP"
     gene            complement(600..1346)
                     /gene="ccmC"
                     /locus_tag="VM713_00400"
     CDS             complement(600..1346)
                     /gene="ccmC"
                     /locus_tag="VM713_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HVH82751.1"
                     /translation="MSWTWFHRLASPPYIYALARRLTPWFAWPAGVLIVAALYGGLVL
                     APPDYQQGDGFRIIYMHAPSAWMSLMVYTTMAVAAAVGLIWRMKVAHAVAASCAPIGA
                     SFTFAALATGSLWGEPMWGTWWEWDPRLTSELILLFLYLGYMGLRGAIEDTQRADRTS
                     AVLAVVGVVNVPIIHYSVTWWNSLHQAPSVMRLGKPTVSTAMLVPLLMMLLGFTLYFV
                     AVLLVRLRGEILNRERSASWVREAFAKGSG"
     gene            complement(1358..2098)
                     /gene="ccmB"
                     /locus_tag="VM713_00405"
     CDS             complement(1358..2098)
                     /gene="ccmB"
                     /locus_tag="VM713_00405"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HVH82752.1"
                     /translation="MPELPALSATLAPATAPAPQQGASVLEAARLVLLRDLTLAFRRR
                     GQLVQPLAFFAIVTTLFPLAISPELARLREVAAGVIWVAALLASLLALESLFRDDAYD
                     GALEQYALSGQPLTGLMFAKTAAHWLLTGLPLAVMAPLAALSLGAPSGSIPGIVASVA
                     VGTIALSLIGSIGAALTLGIRRSGVLLALLTLPLSLPVLIFGARASELAIHGTSYVAP
                     LELLAALAVLGVTLAPLASAAAVRISLE"
     gene            complement(2091..2720)
                     /gene="ccmA"
                     /locus_tag="VM713_00410"
     CDS             complement(2091..2720)
                     /gene="ccmA"
                     /locus_tag="VM713_00410"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HVH82753.1"
                     /translation="MSHEGLEAENLNLWRGERHVLRDVGFAAPRGACLHVAGANGAGK
                     TSLLRAACGLLYPESGRLLWNGRDVHADLPGFPSSLIYIGHEPPLKADLSALENLRFW
                     IGTRRRVGPALLATALQRVGAAAWSERPARTLSAGQKRRVALAALSLAVVPLWLLDEP
                     TTNLDAEGQQLVGTLIEEQLARGGIVLAATHHELPVSPKRVRRLELQLA"
BASE COUNT          531 a         1039 c         1010 g          408 t
ORIGIN      
        1 caggcgtcac caggccttcg cgggactcgc cgtgacgctt gagcgactcg gccaccgccg
       61 gcggcatgta tttctcgtcg tgcttggcga gcacctcgtc ggcaacgaag gtgccgtcct
      121 gcatccggcc gtgggtaacc acacccgccc cttcgcggaa caggtcggga agcacgccgg
      181 tgtagctcac gggaacgttg ctctggaagt cggtcactac gaagtgcacc tcgagacttc
      241 ccgggtcgcg cctgaggctc cccttggtca ccatgccccc gagccggaag cgctcgcccg
      301 ccggcacctt gccggcggtc acctggctcg gatcgaagaa gaaggtgacg ttctgacgaa
      361 aggcgctcaa agcgagcgcg cccgcgacgg cgacgcccgc cacgatcgtc agcaccagag
      421 tcatgcggcg ctgccggggt gtcatgggct gcctccggct gccaaacgcc gccgggcagc
      481 tgcggtcgcc cgcgcgagcg agcgccttgc ccacaccacg ttgagcaccg ccacggcgag
      541 ggtcaccgcg taggccggcc atacgaaggc ggcgtagccg cccatgtcga gaaattcctt
      601 cacccggatc ctttcgcgaa cgcttccctc acccagctcg ccgagcgctc gcggttgagt
      661 atctcaccgc gcagccgcac cagcagcacc gcgacgaagt acagcgtgaa acccagcagc
      721 atcatcagca gcggcaccag cattgcggtc gagaccgtgg gcttgccaag ccgcatcacc
      781 gaaggcgcct ggtggagcga gttccaccag gtgaccgagt agtgaatgat cggcacgttc
      841 accacgccga ccaccgcgag cacggcactg gtgcggtcgg cgcgctgcgt atcctcgatc
      901 gctcctcgca gacccatgta cccgagatag agaaagagca ggataagttc cgaggtgagc
      961 cgcggatccc actcccacca ggtgccccac atcggctcgc cccacagcga gccggtggcg
     1021 agcgcggcga acgtgaacga ggcaccgatc ggagcgcagc ttgcggccac ggcgtgcgcg
     1081 accttcatgc gccagatgag ccccactgcg gcggcgaccg ccatcgtcgt ataaaccatg
     1141 agagacatcc aggcactcgg cgcgtgcatg taaatgatgc gaaacccgtc accctgctga
     1201 tagtccgggg gcgccagcac caggccgccg tacagcgccg cgacgatcaa cacgcccgcc
     1261 ggccaggcga accacggcgt caggcggcgc gccagtgcgt agatatacgg cggcgaggcg
     1321 agtcgatgaa accaggtcca gctcatgtcc cttccattca ctcgagactg atgcgcaccg
     1381 cggcggcgga tgcgagcggt gccagcgtca cgcccagcac cgccagcgcc gcgagcagct
     1441 cgagcggtgc cacgtaggag gtcccgtgga tcgcgagctc gctcgcgcgc gccccgaaga
     1501 tcagcaccgg cagcgacagt ggcagtgtga ggagcgcgag cagcacgccg ctcctgcgga
     1561 tcccgagcgt gagcgccgcg ccgatcgagc cgatgaggct caaggcgatg gtgccgacgg
     1621 cgaccgaggc cactataccg gggatcgagc cggacggcgc tccgagcgag agcgcggcga
     1681 gcggcgccat caccgcgagc ggcaggccag taagcagcca gtgcgccgcg gtcttggcga
     1741 acatgaggcc ggtgagcggc tgcccggaga gcgcgtactg ctcgagcgcg ccgtcatacg
     1801 catcgtcgcg aaacagcgat tcgagcgcga ggagcgaggc gagcagtgcc gccacccaga
     1861 tgacgccggc ggccacttcc cgcaaccgcg cgagctcggg cgagatcgcc agcgggaaga
     1921 gcgtcgtgac gatcgcgaag aaggcgagcg gttgcaccaa ctggccgcga cgccggaacg
     1981 ccagcgtcaa atcgcgtagc agcacgagcc gcgcggcctc gagcaccgat gcgccctgct
     2041 gcggcgcggg tgcggtggcc ggcgccaggg tggctgacag tgccggcagc tcaggcaagt
     2101 tgcagctcca ggcgtcgcac gcgctttggg gataccggca gctcgtggtg cgtcgccgcc
     2161 aacacgatcc cgccgcgcgc gagctgctcc tcgatgagcg taccgaccag ctgctgccct
     2221 tcggcatcga gattggtggt gggctcatcg agcagccaca gcggcacgac cgccagcgac
     2281 agcgcggcga gcgccacccg ccgcttctga cccgcggaca gggtgcgcgc cgggcgctca
     2341 ctccaggcag ccgcgcccac gcgttgcaac gccgtcgcga gcagggccgg tccgacgcgg
     2401 cggcgcgtgc cgatccagaa gcgcagattc tccagcgcgc tcaggtccgc cttcagcggc
     2461 ggctcgtggc cgatgtagat gagcgaggag gggaagcccg gcagatccgc gtgcacgtca
     2521 cggccgttcc agagcagccg gcccgattcg ggatagagca gtccgcaggc ggcgcgcagc
     2581 aagctcgtct tgccggcacc attcgcgccc gcgacgtgca ggcatgcgcc gcgcggcgcg
     2641 gcaaagccga cgtccctgag cacgtggcgt tcaccacgcc agagattgag gttctcggct
     2701 tcgaggcctt catgcgacat cggcgcgcga gtctcgcaaa ttcaggaggt tagcgctatg
     2761 cgtctgccca gagcgtcacg agtgtgagct ccgtcacgca gcacccgtct gcctggggcc
     2821 ggactcgaac cggcacgggg tttcccccaa gggattttcg taccaactgc agctttcgct
     2881 gctgcacgca gtgagccgtg catttgtggt ctggacttta cctttgccat gccgcgcgca
     2941 cgggcggcgt gcgcacggat taggcagggg ccgtcaagtc tctacact
//
LOCUS       DATLKL010000032         5435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_79268, whole genome shotgun sequence.
ACCESSION   DATLKL010000032 DATLKL010000000
VERSION     DATLKL010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79268"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VM713_00415"
     CDS             complement(<1..936)
                     /locus_tag="VM713_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003177920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HVH82754.1"
                     /translation="MADLAAEIHRRRTFAIISHPDAGKTTLTGKLLLFGGAIQMAGTV
                     KGRKAARHATSDWMRLEQQRGISVTSSVMQFPYRDCIVNLLDTPGHEDFSEDTYRTLT
                     AVDSALMVIDCGKGVEERTIKLMEVCRMRDTPIMTFINKLDRDGRTPIELLDEIESVL
                     RIGTAPVTWPIGMGRELRGIYHLAEDRIYAYTAGERGRVGENRTIDGLASAAAREFLG
                     DRAAAFLEEVELVKGATEGFDREAYRAGQQTPVFFGSAINNFGVEELLAAFTRHAPGP
                     LPRAARERTVAAEEPALAGFVFKIQANMDPAHRDRI"
     gene            complement(949..1218)
                     /locus_tag="VM713_00420"
     CDS             complement(949..1218)
                     /locus_tag="VM713_00420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVH82755.1"
                     /translation="MLYLCVGEAFRCRGLGRRLLALLIERAAAAGMKEAFLEVRPSNT
                     AAIRLYLAHGFEQVGMRRGYYQAVSGREDAAVLRLGLRASRPRSS"
     gene            complement(1225..1446)
                     /locus_tag="VM713_00425"
     CDS             complement(1225..1446)
                     /locus_tag="VM713_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82756.1"
                     /translation="MATAPELLGSAPEAIRAMRDADVPDVVAIERSSYQFPWSEGIFR
                     DCLRVGYTCRVATLGRQVAGYGVMSVGAG"
     gene            complement(1430..2131)
                     /locus_tag="VM713_00430"
     CDS             complement(1430..2131)
                     /locus_tag="VM713_00430"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVH82757.1"
                     /translation="MEGARRGEYLEAIGIDLWVPRTAPAARAPRAEAAGETEARWKAL
                     REEVLHCQRCPLHLTRTQGVFGVGPQRADWLVIGEAPGAEEDRRGEPFVGVAGQLLDA
                     MLRAIELDRARNVYIANVLKSRPPGNRDPKPEEVAACLPYLVRQIALLQPKIMLAVGR
                     IAAQTLLGTDAPLGRLRGRVHYFGELNTPLVVTYHPAYLLRTPGDKRKAWEDLKFARN
                     LYQQLTRPVTDGHRS"
     gene            complement(2142..2918)
                     /locus_tag="VM713_00435"
     CDS             complement(2142..2918)
                     /locus_tag="VM713_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HVH82758.1"
                     /translation="MDTQPHARPRSRAIYLLPNLLTTACLFSGFYAVVAAIDKHFAPA
                     GMAVFAAMIFDTLDGRIARLTHTESSFGKEYDSLADMVAFGLAPAIVAYQWGVVRIME
                     FGHYWGRFGWLACFFYAAAAGLRLARFNARAATADKRYFEGLPSPSAAAIVAAFVWFC
                     SEWREPGLTGLIAAFVVTATAGALMVSRFGYPSFKQFNLDRRIKFVYMLFVLLFFVAI
                     AIDPPSILLAMFGVYALSAPLLWAGRRIRRLVRGAPASTG"
     gene            3010..4716
                     /locus_tag="VM713_00440"
     CDS             3010..4716
                     /locus_tag="VM713_00440"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HVH82759.1"
                     /translation="MRLSQYPINTLKETPADAEVVSHQLMLRAGLIRRLAAGLYSWLP
                     LGLRVLQKVERIIREEMNRAGALEMVMPVVQPAELWQESGRWTEYGPELLRIKDRHQR
                     DFVAGPTHEEVITDIVRRELKSYRQLPVNFYQIQTKFRDEVRPRFGVMRAREFFMKDA
                     YSFHLDEESLRAGYRAMYDAYTRIFTRTGLTFRAVRADSGAIGGDVSQEFHVLAASGE
                     DAIAFSDGDDYAANLDAATALPPAEPRPAPRESLRKVATPGARTIEELARFLGIAPAR
                     CVKTLIVDGADGDVVALAVRGDHELNAVKAGKLAGVASPLRMASAERVRAATGTDPGY
                     VGLVGLKCRIYADTSALALADFVCGANERDQHFTGVNWERDVTGAVAADLRNVVEGDP
                     SPTGRGRLRITRGIEVGHIFQLGRKYSEPMKALVLDEAGRETALYMGCYGIGVTRIVA
                     AAIEQNHDERGIIWPQPIAPFQVVLVPINLQKSDRVRAATDRLYTELTDAGIEVLLDD
                     RDARPGVKFADAELLGIPHRLVVAERGLEAGKLEYRHRRDSESTEFPADGALGFMRAR
                     LG"
     gene            4721..5332
                     /locus_tag="VM713_00445"
     CDS             4721..5332
                     /locus_tag="VM713_00445"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HVH82760.1"
                     /translation="MRLAGAVALVTAAVLGSGGARADQQLDPGLRGVVARAIAQAQCF
                     TDQYDSAVWYKLMEPRLRSIVKDHTERLEILREVYCETHRPGEARLPPGLVMALMAVE
                     SHFDRWAVSGAGAVGLMQVMPFWPERLGMRRYELVHVAPNVHMGCAILRYYLGFEHND
                     VRKALARYNGSPGKRDYPDRVLTAWRRWSGADDLGFPAAATTR"
BASE COUNT          859 a         1881 c         1877 g          818 t
ORIGIN      
        1 gatgcggtcg cgatgcgcgg gatccatgtt ggcctgaatc ttgaagacga agccggctag
       61 cgcgggctcc tcggcggcga cggtgcgttc gcgcgcggcg cgcggcaggg gaccgggtgc
      121 gtgccgcgtg aatgccgcga gcagctcctc aaccccgaaa ttgttgatgg cggagccgaa
      181 aaacaccggc gtctgctgtc cggcgcggta ggcctcgcgg tcgaagccct cggtcgcccc
      241 cttcaccagc tcgacctcct cgaggaaggc cgcggcgcga tcgccgagaa actcgcgggc
      301 cgcggcgctc gcgaggccgt cgatggtgcg gttctcgccg acccggccgc gctcgcccgc
      361 cgtgtaggcg tagatgcggt cctcggcgag gtgatagata ccgcgcagct cccgccccat
      421 gccgatcggc caggtcaccg gcgctgtgcc gatgcgcagc acgctctcga tctcgtcaag
      481 cagctcgatg ggggtgcgcc cgtcgcggtc gagcttgttg atgaaggtca tgatcggcgt
      541 gtcgcgcatc cgacatacct ccatgagctt gatcgtgcgt tcctcgaccc ccttgccgca
      601 gtcgatgacc atgagcgccg agtccaccgc ggtgagcgtg cggtaggtgt cctcggaaaa
      661 gtcctcgtgc ccgggagtat cgagcaggtt cacgatgcag tcgcgatagg gaaactgcat
      721 taccgaggag gtcaccgaga tcccgcgctg ctgctcgagc cgcatccagt cggaggtggc
      781 gtggcgtgcc gccttgcgcc ccttgacggt cccggccatc tggatcgcgc cgccgaagag
      841 caacagcttc ccggtgagcg tggtcttgcc ggcgtccggg tgcgagatga tcgcgaaggt
      901 tcgtcgccga tggatctcgg cagccaggtc agccatgagg agcgatgctc agctcgagcg
      961 ggggcgtgag gcgcgcagcc ccagccgcag cacggccgcg tcctcgcgcc cgctaacagc
     1021 ctgatagtag ccgcgccgca tgccgacctg ctcgaagccg tgtgccaggt agagccggat
     1081 agccgcggtg ttggacggac gcacttcgag gaatgcctct ttcatgccag cggccgcggc
     1141 ccgctcgatc agcagcgcca gcagccgcct gcccaggccg cggcagcgga acgcctcgcc
     1201 gacgcacaga tagagaatgt gtgcctagcc tgcgccgaca ctcatcacgc cgtagcccgc
     1261 gacctgccgc ccgagcgtcg ccacgcggca ggtgtacccg acgcgcaggc agtcgcggaa
     1321 gatcccctca ctccagggga actgatacga ggaacgctcg attgcgacga cgtccgggac
     1381 atcggcgtcg cgcatcgcgc ggatcgcctc gggtgccgag ccgagcaact caggagcggt
     1441 ggccatcggt cacgggccgc gtgagctgtt gatacaggtt gcgggcaaac ttgaggtcct
     1501 cccatgcctt gcgcttgtcg ccgggtgtgc gcagcagata ggcgggatga tacgttacga
     1561 cgagcggtgt attcagctcg ccgaaatagt ggacgcggcc ccgcagccgc ccgaggggcg
     1621 catcggtgcc aagcagggtc tgcgccgcga tccgtccgac cgccagcatg atcttcggct
     1681 gcagcagggc gatctgccgc acgaggtacg gcaggcacgc ggccacttcc tccggcttcg
     1741 gatcgcggtt gccgggcggc cggctcttca gcacgttggc gatatagacg ttccgcgcgc
     1801 ggtcgagctc gatggcgcgc agcatcgcat cgagcagctg tcccgcaacg cccacgaacg
     1861 gctcgccgcg ccgatcctcc tcggcccccg gcgcttcgcc gatcaccagc cagtcggcgc
     1921 gctgcgggcc tacgccgaac acgccctggg tgcgcgtgag atgcagcgga cagcgctggc
     1981 agtggagcac ttcctcgcgc agcgccttcc acctcgcctc agtctctccg gcagcctcgg
     2041 cgcggggcgc acgcgccgcg ggcgctgtgc gcggcaccca caggtcgatg ccgatcgcct
     2101 cgagatattc cccgcgccgc gccccctcca cggtcgggcc ctcagccggt cgacgccggc
     2161 gcgccgcgca ccaagcggcg gatgcgccgg cccgcccaca ggagcggggc cgagagcgcg
     2221 tacacgccga acatcgcgag caggatgctc ggcggatcga tcgcgatcgc cacgaagaac
     2281 agcagcacaa acagcatgta gacgaacttg atgcgtcggt cgagattgaa ctgcttgaag
     2341 ctcgggtagc cgaagcgact caccatcagg gcgcccgcgg tcgcggttac tacgaaggcc
     2401 gcgatcagcc ccgtgaggcc cggctcacgc cactcgctgc agaaccacac gaaagccgcg
     2461 acgatcgccg cggccgacgg gctcggcagg ccctcgaagt agcgcttgtc ggcggtcgcc
     2521 gcccgggcgt tgaagcgcgc caggcgcagc cccgccgccg ccgcatagaa gaagcaggcc
     2581 agccagccga accggcccca gtagtggccg aactccatga tgcgcaccac gccccactgg
     2641 taggccacga tcgcgggcgc gaggccgaat gccaccatgt cggcgagact gtcgtactcc
     2701 ttgccgaagg agctctcggt gtgcgtgagg cgcgcgatgc gcccgtcgag ggtgtcgaag
     2761 atcatcgctg cgaacacggc catgccggcc ggcgcgaagt gcttgtcgat cgccgccacc
     2821 acggcgtaga aacccgagaa caggcaggcg gtcgtgagca ggttcggcag caggtaaatc
     2881 gcccggctgc gcggccgggc gtggggctgg gtgtccatct cgatagccaa tcggcgggca
     2941 tcttatcagg ggcctcctgc taagatgcca actccttgtt ttttggcccc gtaacagaag
     3001 ctgcgcccga tgcgtctctc gcaatacccg atcaacacct tgaaggagac ccccgcggat
     3061 gcggaggtcg tgagtcatca gctcatgctg cgcgccggcc tcatccggcg tctggcggcg
     3121 ggcctgtaca gctggctacc gctcggcctg cgggtcctgc agaaggtcga gcgcatcatt
     3181 cgcgaagaga tgaaccgcgc gggagcgctc gagatggtga tgccggtggt tcagccggcc
     3241 gagctgtggc aggagtcggg gcgctggacc gaatacggac cggagctgct gcggatcaag
     3301 gaccgccacc agcgcgactt cgtcgccggc cccacccatg aagaggtgat caccgacatc
     3361 gtgcggcgcg agctgaagag ctaccggcag ctgccggtga acttctacca gattcagacc
     3421 aagttccgtg acgaggtgcg gccgcgcttc ggcgtcatgc gggcccgcga gttcttcatg
     3481 aaggacgcct actcctttca cctcgacgag gagtcgctgc gcgccggtta ccgcgccatg
     3541 tacgacgcct atacgcgcat cttcacccgc accgggctca ccttccgcgc ggtgcgtgcc
     3601 gactccggcg cgataggggg cgacgtcagc caggagttcc acgtgctcgc cgcctcgggc
     3661 gaggacgcga tcgccttctc ggacggagac gactacgcgg ccaacctcga tgcggccacc
     3721 gcgctcccgc ccgccgagcc acgcccagcg ccgcgcgagt cgctccgcaa ggtagcgacc
     3781 cccggggcgc gcaccatcga ggagctggcg cgttttctcg ggatcgctcc cgcgcgctgc
     3841 gtgaagactc tcatcgtgga cggtgcggac ggcgacgtgg tggcgctcgc ggtgcgcggc
     3901 gaccacgagc tgaacgccgt caaggccggt aagctcgcgg gcgtcgcgag cccgttgcgc
     3961 atggcgagcg ccgagcgggt gcgcgcggca accggcaccg accccggcta cgtcgggctc
     4021 gtcggcctca agtgccgcat ctacgccgac acgtccgcgc tcgcgctcgc ggacttcgtg
     4081 tgcggcgcca acgagcgcga ccagcacttc accggcgtca actgggagcg cgatgtgacc
     4141 ggcgcagtcg ccgcggatct gcgcaatgtc gtcgagggcg atccgagccc cacgggccgc
     4201 gggcgcctca ggatcacgcg tggcatcgag gtgggacaca tcttccagct cgggcgcaag
     4261 tacagcgagc cgatgaaggc cctggtgctc gacgaggccg ggcgggaaac ggccctgtac
     4321 atgggctgtt acggcatcgg tgtgacgcgc atcgtggcgg cggccatcga gcagaatcac
     4381 gatgagcgcg gcatcatctg gccgcagccg atcgctccct ttcaggtggt gctggtgccg
     4441 atcaatctgc agaaatctga ccgtgtccgc gccgccaccg accgcctgta caccgagctc
     4501 accgacgccg ggatcgaagt cctgctcgac gaccgcgacg cccgtcccgg cgtcaaattc
     4561 gccgacgccg agctcctcgg catcccgcat cggctggtgg tggcggaacg cgggctcgag
     4621 gccggcaagc tcgagtaccg gcaccgccgc gacagcgaga gcaccgagtt tccggctgac
     4681 ggtgcgctcg gcttcatgcg cgcgcgcctc ggctaaggcg atgcggctcg cgggcgccgt
     4741 cgcgctcgtg accgccgcgg tgctcggcag cggcggcgcg cgcgccgatc agcagctcga
     4801 tccggggctg cgcggcgtgg tcgcgcgcgc catcgcccag gcgcagtgct tcaccgacca
     4861 gtacgattcg gcggtctggt acaagctcat ggagccgcgg ctgcgctcca tcgtgaagga
     4921 tcacaccgag cggctcgaga tcctcaggga ggtctattgc gagacgcacc gccccggcga
     4981 agcgcgcctg ccgccggggc tggtgatggc gctgatggcc gtcgagagtc acttcgatcg
     5041 ctgggcggtc tccggggccg gggcggtcgg cctgatgcag gtgatgccct tctggcccga
     5101 gcggctcggg atgcgccgtt acgagctggt gcacgtcgcg ccgaacgtgc acatgggctg
     5161 tgcgatcctg cgctattacc tcggcttcga gcacaacgac gtgcgcaagg cgctcgcccg
     5221 ctacaacggc agcccgggca agcgcgacta cccggaccgc gtgctcaccg catggaggcg
     5281 ttggagcggc gcggacgacc tgggcttccc ggccgccgcg actacgcgtt agtcgatcag
     5341 tgcgtcgtgg ccgccgcgtg tttccccgtg cggtgcggct cgagcatgct cgccaccccc
     5401 tcgcggcgtg aatcggccgc tccctggtaa gtgcc
//
LOCUS       DATLKL010000033         2035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_56760, whole genome shotgun sequence.
ACCESSION   DATLKL010000033 DATLKL010000000
VERSION     DATLKL010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56760"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..686)
                     /locus_tag="VM713_00450"
     CDS             complement(258..686)
                     /locus_tag="VM713_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82761.1"
                     /translation="MSGDRRGWRIALVPEALLNPPRRGRAGLPDGLDVLDVLGSSGYG
                     VLHMPPPGRHGLLLAVIADQVAEYAHHGYAVVAIGMPAKSGDGLHWRRLAPLLRHRGV
                     PLPPRHLIRPQVDAAAEARRLTAFLAGYDLPPEEQHRWRV"
     gene            complement(683..1285)
                     /locus_tag="VM713_00455"
     CDS             complement(683..1285)
                     /locus_tag="VM713_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82762.1"
                     /translation="METNTPRKVALLANCLLNQNAKVCEGAHYRGVVNPVVAALRSRG
                     YELLQLPCPELAFAGVRRWWAVYEQYDTPAYRAHCKRLAQAIAPLIEQHLRRGAEVIL
                     IGLDGSPSSGVRFTSSKPDWGGRPNRPEDDWEIVPRRGIWIEELEAELARRGLPPLPA
                     TGWALDMGRFDEAGARRDLEQFLDSRADAPGGRARKSAAK"
     gene            complement(1292..>2035)
                     /locus_tag="VM713_00460"
     CDS             complement(1292..>2035)
                     /locus_tag="VM713_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVH82763.1"
                     /translation="DLGAVAPLPGVRNMNVESLFEYGSRVGFREIIRLLKSAAAPATI
                     YAVGTALERNPEAATELAASGFEVACHGQRWIDYQFVPEDVERHDMLRNVEVITRLTG
                     RRPVGWYTGRPSPNTRRLVVETGGFLYDSDAYNDDLPYWTQVSGQRHLVIPYSFDNND
                     SRLQRGGDFSTGDEFFTYCRDAFDWLYRLGCEGRPRMMSVGLHGRIIGRPGRIGALAR
                     LLEHIQRHDGVWLCSRAAIAQHWRMHHPE"
BASE COUNT          323 a          717 c          683 g          312 t
ORIGIN      
        1 ctttggcgat gcgcaggcgc accccgtcct tgctcggaag aagtccgccc ccgtcaacga
       61 acttgccttc cctggcgagc ctttcgatga acttgcccat ggcctccatc agcgccttgg
      121 gggggccggc ctcgcgataa gactcggaat ggcgaacgaa cgtcatgtac ttcatggatc
      181 ctccgttggg cctcgctcac aagacgaacg aggccgcacc ggatcgacac gctcgcgcgt
      241 cgtgtccgca cccggggcta cacgcgccag cggtgctgct cctccggcgg cagatcatag
      301 ccggccaaaa aggcagtgag ccgccgcgcc tcggccgcgg cgtcgacctg cggccggatg
      361 agatgacggg gtgggagcgg cacgccgcgg tgtcgcagca gcggcgccag ccgacgccag
      421 tgcagcccat cgccggactt cgcgggcatc ccgatagcga ccaccgcata gccgtggtgc
      481 gcatactctg caacctgatc ggcaatcacg gcgagcagca gcccgtgcct gccgggcggc
      541 ggcatgtgca gcaccccgta gccgctcgac ccaagcacgt cgagcacgtc gagcccgtca
      601 ggcagccccg cgcggccccg ccgcggggga ttgagcagcg cctcgggcac cagcgcaatc
      661 cgccagccgc gtcggtcgcc gctcacttcg ccgccgattt cctggcgcgg ccgcccggag
      721 catccgcgcg gctgtccagg aactgctcca ggtcccggcg tgcgccggcc tcatcgaagc
      781 ggcccatgtc gagcgcccag cccgtggccg gtagcggcgg cagtccgcgg cgcgcgagct
      841 cggcctccag ctcctcgatc cagatgcccc ggcgcggcac gatctcccag tcgtcctcgg
      901 gacggttggg gcgcccgccc caatcgggct tactggaggt gaagcgcacg ccgctcgagg
      961 gactgccgtc gagcccgatc agaatcacct cggcgccgcg gcgcagatgc tgctcgatga
     1021 ggggcgcgat cgcttgcgcg aggcgcttgc agtgagcccg ataagccggc gtgtcgtact
     1081 gctcatagac agcccaccag cgtcgcacac cggcgaaggc gagctcgggg cagggcagct
     1141 gcagcagctc atacccccgg ctgcgcagtg ccgcgacgac cggattcacg acgccgcgat
     1201 agtgtgcgcc ctcacatacc ttggcgttct gattcaacag gcaattggcg agcagcgcca
     1261 ccttgcgcgg tgtgttcgtc tccatagcgt ttcactctgg gtgatgcatg cgccagtgtt
     1321 gtgcgatggc tgcgcgactg cacagccaga caccgtcgtg acgctgtatg tgctcgagca
     1381 ggcgcgccaa tgcgccgatg cggccggggc gtccgatgat acggccgtgc aggccgacgc
     1441 tcatcatgcg cggccgccct tcgcaaccca ggcgatagag ccagtcgaac gcatcacggc
     1501 agtaggtgaa gaactcatcg ccggtgctga aatcgccgcc gcgctgcagg cggctgtcgt
     1561 tgttgtcgaa gctgtagggg atgacgaggt ggcgctggcc cgagacctgc gtccagtacg
     1621 ggaggtcatc gttataggcg tcgctgtcgt agaggaagcc gccggtctcg acgaccagcc
     1681 gccgcgtgtt ggggctcggg cggcccgtat accagccgac gggcctgcgg cccgtcagtc
     1741 gcgtgatgac ctcgacgttg cgcagcatgt cgtggcgctc gacatcctcc ggaacgaact
     1801 gatagtcgat ccagcgctgc ccatgacagg ccacctcgaa gccgctcgcc gcgagctcgg
     1861 tggcggcctc cgggttgcgc tcgagcgccg tccccacggc gtagatggtc gccggcgccg
     1921 ccgcgctctt cagcaggcgg ataatctccc ggaagcccac gcggctgccg tattcgaaca
     1981 gcgactcgac gttcatattg cgcacgccgg gcagcggcgc gacggcgccg agatc
//
LOCUS       DATLKL010000034         3337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_101918, whole genome shotgun sequence.
ACCESSION   DATLKL010000034 DATLKL010000000
VERSION     DATLKL010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101918"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /gene="mraZ"
                     /locus_tag="VM713_00465"
     CDS             <1..368
                     /gene="mraZ"
                     /locus_tag="VM713_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HVH82764.1"
                     /translation="AQGRLVVTVDKDQCLLIYPLPEWEQIERKLMSLPSLNPTARRLQ
                     RLMVGHATDLPLDGHGRVLLPAELREFAKLGRHGMLIGQGNRFELWDESRWNERRDLW
                     LKNEEVATDLPSELESLSL"
     gene            440..1348
                     /gene="rsmH"
                     /locus_tag="VM713_00470"
     CDS             440..1348
                     /gene="rsmH"
                     /locus_tag="VM713_00470"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HVH82765.1"
                     /translation="MLAAEALAGLALETDGYYVDATFGRGGHTALILQALGREGRVLA
                     VDRDPQAVAAGRERFSNEMRLTLVHASFADLGALVPLHADDRACRGVLFDLGVSSPQL
                     DDARRGFSFRADGPLDMRMDPSRGEPVSAWLARAGVDEIREVIASFGEERFARRVARA
                     IVEARERRPLARTAELAEVVAQAVRTREPGKHPATRTFQALRMFINDELGQLERGLRG
                     ALATLAAGGRLVVITFHSLEDRAVKRFMQRESQPAAVFAQLPVLPPQARPTLKIIGRK
                     SRPTEAELKRNPRARSALLRVAEKLS"
     gene            1345..1623
                     /gene="ftsL"
                     /locus_tag="VM713_00475"
     CDS             1345..1623
                     /gene="ftsL"
                     /locus_tag="VM713_00475"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HVH82766.1"
                     /translation="MSERTVLALTVPLLWLAVLASAAGAIYCEHRARELFIQLEALNA
                     QRDNLQIEWGQLQLEQSAWSSHAFVERVASTRLHMAIPPPQSIEVVSP"
     gene            1620..>3337
                     /locus_tag="VM713_00480"
     CDS             1620..>3337
                     /locus_tag="VM713_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HVH82767.1"
                     /translation="MKASRVRGNPAPRSFRWRARLLLALLTLACAALLWRAVDLQLVD
                     HGFLARQGDARFSRVLQVAAHRGTITDRYGEPLAVSTPVDSIWVNPEELALADEQIPR
                     LASALALDRQELARRITSNLEREFLYLARGRQPAEAAAIKALGIPGVYTSREYRRYYP
                     AGEVSGHLLGFTNVDDAGQEGLELAFDHWLAGEPGAKRVIQDRYGRIVEDVESIRPAR
                     PGRDLVLSIDLRIQYLAYRELKAAVRDQRARAGSIVVLDVTTGEVLAMVNQPAFNPND
                     REQIQAADYRNRAATDIFEPGSSIKPFWVAAALAAGKYDTRSVIDTSPGFFKVGVKIF
                     EDEHNFGAIDIATVLAKSSNVGMAHIALALAPQQAWTTLTRLGFGEVTSSGYPGESAG
                     LLPAYSQWRPIGIVTMSHGYGLSVTPLQLAHAYATIGALGIARPVSFLAADAAVSGTR
                     VLEGPSCHELLAMLESVVAAEGTGKLAAIPGYRVSGKTGTAWKATAGGYSTDRYMAVF
                     GGVAPVTAPRLAAVVVIDEPSAGQHMGGQVAAPVFSRVVGGALRLLAVAPDQPVRAPE
                     ELPANDS"
BASE COUNT          497 a         1166 c         1156 g          518 t
ORIGIN      
        1 tcgcccaggg aagattggtc gtcaccgtcg acaaggacca gtgtctcctc atttatcccc
       61 tgcccgagtg ggaacagatc gaacgcaagc tgatgagcct gccgagcctc aatccgacgg
      121 ctcggcggct gcagcggctg atggtgggcc acgcgaccga tctgcccctc gacggacacg
      181 gcagggtgct gctgccggct gagctgcgtg aattcgccaa gctcgggcgg cacgggatgc
      241 tgatcggcca gggcaaccgc ttcgagctgt gggacgagtc gcgatggaac gaacggcgcg
      301 acctgtggct caagaatgag gaggtggcga ccgatctgcc gtccgagctc gagtcgctgt
      361 cactgtaaat cggccacggg gccgcgatga ggttttgggc cagaggcgcg ccgcttctat
      421 gggggatgag cacacaccgg tgcttgccgc ggaggcgctc gcggggttgg ccctggaaac
      481 ggacggttac tatgtcgatg cgacgttcgg gcgcggcggc cacacggcac tcatactcca
      541 ggccctgggt cgagagggcc gcgtgctcgc agtcgaccgc gatccgcagg ccgtcgcggc
      601 cgggcgcgag cgcttttcca acgaaatgcg gcttacgctc gtgcacgcgt cgtttgccga
      661 tctcggcgcg ctcgtgccgc tacacgcaga cgatcgtgct tgccgcggcg tgctcttcga
      721 cctcggggtg tcctcgccgc agctcgacga cgcgcgccgc ggcttcagct tccgcgccga
      781 cggcccgctc gacatgcgca tggacccgtc acgcggcgaa ccggtctcag cgtggctcgc
      841 tcgggcgggc gtcgacgaga tacgcgaggt gatcgccagc ttcggcgagg agcgtttcgc
      901 gcgccgcgtg gcccgcgcga tcgtcgaggc gcgtgagcga cgtcctctcg cgcgcaccgc
      961 cgagctcgcg gaggtggttg cgcaggccgt gcgcacgcgt gagcccggca agcacccggc
     1021 gacgcgcacc ttccaggcgc tgcgcatgtt catcaacgac gagctcggac agctcgagcg
     1081 ggggctgcgc ggcgcgcttg ccacactcgc cgccggcgga cggctggtgg tcatcacgtt
     1141 ccattcgctc gaggaccgtg cggtgaagcg cttcatgcag cgcgagtctc agcccgctgc
     1201 agtcttcgcg cagctgccgg tgctgccgcc gcaggcacgg ccgacactca agatcatcgg
     1261 acgcaagagc cggccgacgg aagcggagct gaagcgcaat ccacgcgcgc gcagcgcgct
     1321 gctgcgcgtg gcggagaagc tctcatgagc gagcggacgg tgctggcgct caccgtgccg
     1381 ctcctgtggc tcgcggtgct cgcctcagcc gccggcgcca tctactgcga gcaccgcgcg
     1441 cgcgagctct tcatccagct cgaggcgctc aacgcgcagc gtgacaacct gcagatcgag
     1501 tggggccagc tgcagctcga gcagagcgcc tggtcgagcc acgcgttcgt cgagcgcgtc
     1561 gcgagcacgc ggctgcatat ggcgatcccg ccgccgcaga gcatcgaggt cgtctcgcca
     1621 tgaaggcgag ccgcgttcgt ggcaaccccg cgccgcgctc gttccgctgg cgcgcgcgcc
     1681 tgctgcttgc gctcctcacg ctcgcctgcg cggcgctgct gtggcgtgcg gtggatctgc
     1741 aactggtcga tcacggcttt ctcgcccgcc agggagatgc gcgcttctcg cgcgtactgc
     1801 aggtcgccgc ccatcgcggc actatcaccg accgatacgg cgagccactg gcggtgagca
     1861 cgccggtcga ctcgatttgg gtgaacccgg aggagctggc gctcgctgat gagcagattc
     1921 cgcgcctcgc gagcgcgctc gcgctcgacc gccaggagct ggcacggcgc atcaccagca
     1981 atctcgagcg cgagttcctc tatctggcgc gcgggcgcca gccggccgag gccgccgcga
     2041 tcaaggcgct cgggattccg ggcgtctaca cctcgcgcga gtatcgccgc tactacccgg
     2101 cgggcgaggt gagcgggcac ctgctcggct tcacgaatgt cgacgatgcc gggcaggagg
     2161 gcctcgagct cgccttcgac cactggctcg ccggagagcc gggcgcgaag cgcgtcatcc
     2221 aggatcgcta cggacgcatc gtcgaggacg tcgagagcat ccgcccggcg cgcccgggac
     2281 gggacctggt gctatcgatc gatctgcgca tccagtacct cgcctatcgc gagctcaagg
     2341 cggcggtgcg cgatcagcgg gcgcgcgccg gctcgatcgt ggtgctggac gtgacgacgg
     2401 gcgaagtgct cgccatggtg aaccagcccg ccttcaaccc caacgaccgc gagcagatcc
     2461 aggccgctga ctaccgcaac cgcgccgcca cggatatctt cgagcccggc tcctcgatca
     2521 agcccttctg ggtggccgca gccctcgccg cgggcaagta cgacacgcgc agcgtcatcg
     2581 atacctcgcc gggcttcttc aaggtgggcg tcaagatctt cgaggacgag cataatttcg
     2641 gcgcgatcga catcgccacc gtgctcgcca agagctccaa cgtcggcatg gcccatatcg
     2701 cgctcgcgct cgctccgcag caggcgtgga ccacgctcac aaggctcggc ttcggcgaag
     2761 ttacgagcag cggttatccg ggcgagtccg cgggactgct gccggcctat tcgcagtggc
     2821 ggccgatcgg catcgtcacc atgtcgcacg gctatggatt gtcggtgacc ccgctgcagc
     2881 tcgcccacgc ctacgccacc attggcgcac tcggcatcgc ccggccggtg tccttcctcg
     2941 ccgccgacgc tgcggtgagc ggcacgcgcg tactcgaggg accgagctgc cacgagctcc
     3001 tcgccatgct tgagtcggtc gtcgcggccg aaggcaccgg caagctcgcg gcgattcccg
     3061 gctaccgcgt ctccggcaag accggcaccg cgtggaaagc gaccgcgggc gggtactcaa
     3121 cggatcgcta catggcggtg ttcgggggcg tcgctcccgt gaccgcccca aggctcgccg
     3181 ccgtcgtcgt gatcgacgag ccgagcgccg gccagcacat gggcggtcag gtcgcggcgc
     3241 ccgtattctc ccgcgtcgtg ggtggggcgc tgcggctgct cgcggtcgcg ccggatcagc
     3301 ccgtacgcgc gccggaggag ctgccggcga acgactc
//
LOCUS       DATLKL010000035         2875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_11604, whole genome shotgun sequence.
ACCESSION   DATLKL010000035 DATLKL010000000
VERSION     DATLKL010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11604"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VM713_00485"
     CDS             <1..318
                     /locus_tag="VM713_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH82768.1"
                     /translation="SSRLKGLQVRFPCVGEVRNLGAMVAIELVKNMRADAPDAELTKA
                     LVQAAGRRGLIILSCGVYANVIRFLAPLTISDGLLKEGCHVFEQALEEVTGGADVAAR
                     AVG"
     gene            complement(326..628)
                     /locus_tag="VM713_00490"
     CDS             complement(326..628)
                     /locus_tag="VM713_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82769.1"
                     /translation="MTESLADTFDAAFSKAVDLGNRIADKDKDADLWDIADGLLAGAL
                     QYWLYSRQPCGDPRCQDCLPISTAQGRMAELKRLIEQLASESEYFHTPTDANAGRA"
     gene            705..>2875
                     /locus_tag="VM713_00495"
     CDS             705..>2875
                     /locus_tag="VM713_00495"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVH82770.1"
                     /translation="MAPASPEAADSAAPRPQSPRRRAWLPRALRGVGARLALGLAAVA
                     AVLIVGESVATRTSQEALQAMRSMQNEHEPLASRASAVLVRLVDYDRAVGEYVQARNG
                     ADFAAITTAGAALEDALSGYYDGTPRPPVTRPALELRAALSRHIAAGRAFASRTAQRG
                     QWVDERRAMLAAVYHRIVTAGGAGLAINGTQVLARRSLAELQAAIDGVRGSEDPSVID
                     SRERDFGAALEAHAAELRSSPGRAWLSMVREDFAQAVRLRQAIARYDQVSGGDWHKLL
                     EESAALTAGVQELLQKPTRGGLVQAAQHAATAAEVSERTLAFSGAAVLGLLLLVSVLL
                     TLSISLPVWRLTAATRLLAGGDRSARAPRGGSAEIYELAESFNTMADRIASAEGELRA
                     HQAELERHVAERTRALHYLAYHDPLTQLPNRRQLSARLGTALKGAGPRQLLALLFVDV
                     DNFKSINDTLGHSFGDRVLQHLARRLHAAIGGAGLLARLGGDEFTVLLEDVQSSDEVA
                     GCANRILSALQQPLTVDGRLLTTSASVGASFYPDHAADAEGLLRAADVALFRAKELGR
                     NRFALYSPELYDAAAQRFRIEQSLRRAVERGELLLMFQPIVDLRAFETTGLEALLRWR
                     RPDGRIAPAAEFIGVAERSGLIHELTGWVLRSATATAARWLAQGWQGASVAVNVSPPQ
                     FFESDFVEQVARTLEASGLPPSALEIEITESVVQTGSATIES"
BASE COUNT          400 a         1022 c         1032 g          421 t
ORIGIN      
        1 agctcgcgtc tgaagggcct gcaggtgcgc ttcccctgcg tgggcgaggt gcgcaacctc
       61 ggcgcgatgg tggcgatcga gctggtgaag aacatgcgcg ccgacgcgcc ggacgcggag
      121 ctgaccaagg ccctcgtgca ggccgccggc cgtcgcgggc tcatcattct ctcgtgcggc
      181 gtctatgcca acgtgatccg cttcctcgca ccgctgacga tttccgacgg cctcctcaag
      241 gagggctgtc acgtgttcga gcaggcgctc gaggaagtca cgggcggcgc ggacgtggca
      301 gcgcgcgccg tcggctgaga gtcgactatg cgcgccctgc gttcgcgtcg gtcggcgtgt
      361 ggaagtactc gctctccgag gcgagctgct cgatgaggcg cttcagctcc gccatgcgcc
      421 cctgcgcggt gctgatcggc aggcagtcct gacagcgcgg gtcgccgcac ggctgacgcg
      481 agtacagcca gtactgtagg gcgcccgcca acaacccgtc agcgatatcc cacagatccg
      541 cgtctttgtc cttgtcggca atgcggttgc ccagatcgac ggccttggag aaggccgcat
      601 cgaacgtgtc agcgagggat tcggtcattg cgtcgcggtt cgccctaaat cggtcacgct
      661 agtatagggc aaaggagcat cgcgccccca acagcttatg ttgcatggcc ccagccagcc
      721 ctgaagctgc cgattccgca gcgccgcggc cgcagtcgcc gcgacgtcgt gcctggctgc
      781 cgcgcgcgct gcgcggcgtc ggcgcgcgcc tcgcgctcgg gctcgcggcg gtcgccgcgg
      841 tgctcatcgt gggcgagagc gttgcgaccc gcacctccca agaagcgctc caggcgatgc
      901 gcagcatgca gaacgagcat gagccgctcg cgagccgcgc gagcgccgtg ctggtgcgcc
      961 tcgtcgacta cgaccgtgcg gtgggcgagt acgtgcaggc gcgcaacggc gccgacttcg
     1021 ccgccatcac gaccgcgggc gctgcgctcg aggacgcgct gagcggctac tacgatggca
     1081 ccccgcggcc gccggtgacg cgcccggcgc tcgagctgcg cgccgccctc agccggcaca
     1141 tcgccgcggg gcgcgcgttc gcgagccgca cggcgcagcg tggccaatgg gtcgatgagc
     1201 gccgcgccat gctcgccgcg gtgtatcacc gcatcgtgac cgcgggcggc gcgggactcg
     1261 cgatcaacgg cacgcaggtg ctggcgcggc gctcgctcgc ggagctgcag gccgccatcg
     1321 acggcgtgcg cggcagcgag gacccgagcg tcatcgacag ccgcgagcgc gacttcggcg
     1381 ccgcgctcga ggcgcacgcc gcggagctgc gcagctcgcc ggggcgcgcc tggctcagca
     1441 tggtgcgcga ggatttcgcg caggcggtgc gcttgcgcca ggcgattgcg cgctatgacc
     1501 aggtgagcgg cggcgattgg cacaagctcc tcgaggagag cgcggcgctc accgccggcg
     1561 tgcaggagct gttgcagaag cccacgcgcg gcggcctggt gcaggcagcg cagcatgccg
     1621 ccacggcggc cgaagtctcc gagcgcacgc tcgccttctc cggggctgcg gttcttggcc
     1681 tcctgctgct cgtgtcggtg ctgttgacgc tcagcatcag cctgcccgtg tggcggctca
     1741 cggccgcaac gcgcctcctg gccggcggcg accgcagtgc gcgcgccccg cgcggcggct
     1801 cggcggagat ctacgagctc gcggaatcct tcaacaccat ggccgaccgg atcgcgagcg
     1861 ccgagggcga gctgcgcgcg caccaggccg agctcgagcg gcacgtcgcc gagcgcaccc
     1921 gggcgctgca ctacctggcc tatcacgacc cgctgaccca gctgcccaat cggcgacagc
     1981 tgtcggcgcg gcttggcacc gcgctgaaag gggcgggtcc gcggcagctg ctcgcgctgc
     2041 tgttcgtcga cgtcgacaac ttcaagtcga tcaacgatac gctggggcac agcttcggcg
     2101 atcgggtgct gcagcacctc gcccgccggt tgcacgccgc catcggcggc gccggattgc
     2161 tcgcgcggct cgggggcgat gaattcacgg tgctgctcga ggatgtgcag tcgagcgatg
     2221 aggtggccgg ctgcgccaac cgcatcctga gcgcgctgca acagcctctg acggtcgacg
     2281 ggcggctgct cacgaccagc gccagcgtcg gcgcgagctt ctatcccgat cacgccgccg
     2341 atgctgaggg cctgctgcgc gccgccgacg tggcgctgtt tcgcgccaag gagctcgggc
     2401 gcaaccgctt tgcgctgtac agccccgagc tctacgacgc ggccgcgcag cgcttccgca
     2461 tcgagcagtc gctgcggcgc gccgtcgagc gcggcgagct gctgctcatg ttccagccga
     2521 tcgtggacct cagggccttc gagaccacgg gactcgaggc gctgctgcgc tggcggcggc
     2581 ccgacggacg catcgcgccc gccgcggagt tcatcggggt cgccgagaga tccggcctca
     2641 tccacgagct caccggctgg gtgctgcgct ccgcgaccgc caccgcggcc cgctggcttg
     2701 cccagggctg gcagggcgcg agcgtcgcgg tgaacgtctc gccgccgcag ttcttcgaga
     2761 gcgacttcgt cgagcaggta gcgcgcacgc tggaagcgtc cgggctgccg ccgagtgcgc
     2821 tcgagatcga gatcaccgaa agcgtcgtgc agaccggcag cgccaccatc gagtc
//
LOCUS       DATLKL010000036         2672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_124443, whole genome shotgun sequence.
ACCESSION   DATLKL010000036 DATLKL010000000
VERSION     DATLKL010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124443"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VM713_00500"
     CDS             <1..316
                     /locus_tag="VM713_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVH82771.1"
                     /translation="ALALTPDFVLLDVRSADDYAHGHVPGAVHLPHRKIIESRLAGYP
                     ADTVFVVYCVGPHCNGAQRAALRLAQLGRPVKLMIGGMTGWLDEGFEIERTAEPARAV
                     SA"
     gene            411..2297
                     /locus_tag="VM713_00505"
     CDS             411..2297
                     /locus_tag="VM713_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HVH82772.1"
                     /translation="MSLLLKIFAVLLLVLLLLTGYGLWATRPPASSTPVGALSATQAE
                     VAGSLPTIEESTLLTAQRLARLATTPEEVPLAHTAVQLADHELDLAFAGALRHLEAHP
                     PVLSPEALEIQDRLTRAQRQLASESERVKRLTDALGKAADADKPPIQDQLDLAQAQQQ
                     LDQDEVTEANDDLMRAGGNEHQRVQMMQQEHEAAAQSRQAAPAPAASPLGSLKGLVGK
                     VRQWMALHRKERWLGQAQAHAVESAAQLEAGRQQIATDLAARKEQAGAVSTATPPEAA
                     VPTSSMMRATAGAQATGTIARPPSTSGAPPAASGSALAARTREIAAEQSRLTLRGQRI
                     TARKRLAETYGQWSAVVGIQANALLHSALADVAVVLGALVLLLFIDRWLETLLGRAPV
                     DRRQIATLRSVVGVSCQLVGIVVILLVLIGLPGQLGTMIGLAGAGLTVALKDFIIGFL
                     GWFVLMGKHGMRVGDWVEINGVSGEVVELGIFHTVLLETGNWADAGHPTGRRVTFTNA
                     FAIEGHYFNFSTTGQWLWDELLVMVPFDRDARAIADAIQKEVTEATAESSREAEAEWQ
                     RAARGRKLGFTAQPGIAIRPAPGGGGVEIAVRYVTRAAERFALRSRLYQSAVQLLSQP
                     KPAA"
     gene            2301..>2672
                     /locus_tag="VM713_00510"
     CDS             2301..>2672
                     /locus_tag="VM713_00510"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HVH82773.1"
                     /translation="MSARLPAVFLGHGNPMNAVRENAWTRAWAAFGAALAPKPRAVLA
                     ISAHWYIVRTAVTAMAAPRTIHDFGGFPRELFAMRYPAPGDPTLAVRVAELLAPLPVV
                     ADASWGLDHGTWSVLCHVFPEA"
BASE COUNT          410 a          951 c          896 g          415 t
ORIGIN      
        1 agcgctcgcc cttacgcccg acttcgtgct gctcgatgtg cgctcggcgg atgactacgc
       61 acacggccac gtgccgggtg ccgtgcacct gccacatcgc aagatcatcg agtcgcggct
      121 tgccgggtat cccgcggaca ccgtgttcgt cgtctactgt gtgggcccgc attgcaacgg
      181 tgcgcagcgc gccgcgctgc gcctcgcgca gctcgggcgc ccggtaaagc tcatgatcgg
      241 cggaatgacg ggctggctcg atgagggctt cgagatcgag cgcacggcag agccggcccg
      301 cgccgtcagc gcctagcacc cgccattggc gcggcgcgcg ggcaggaggc ccgcggaaga
      361 ccctgcgcgc catggcatca taggggcccg ttcctctcga tcgcgccgct atgagcctat
      421 tgctcaagat cttcgctgtc ctgctcctcg ttctcctcct cctcaccggg tatggcctct
      481 gggccacacg cccgccggct tcttcgactc cggtcggcgc gctgagcgcc acacaggcgg
      541 aggtcgctgg ctcgctgccg accatcgaag agagcacgct cctcaccgcg cagcgcctcg
      601 cgagactcgc gacgaccccc gaggaggtgc cgctcgccca caccgccgtg cagcttgctg
      661 accatgagct cgacctcgcc ttcgccggcg cgctgcggca cctggaagcg caccctcctg
      721 tgttgagccc ggaggcgctc gaaattcagg accggctcac cagagcgcag cgccagctcg
      781 cgagcgagag cgagcgcgtc aagcgcttga ccgatgcgct cggcaaggcg gcggatgccg
      841 acaaaccccc gatccaggat cagctcgatc tcgcgcaggc gcagcagcag ctcgaccaag
      901 acgaggtcac cgaggccaac gacgatctga tgcgcgccgg tggcaacgag caccagcgcg
      961 tccagatgat gcagcaggag cacgaggcgg ccgcgcagag ccgtcaggcg gcgcccgccc
     1021 ccgcagcgag cccgctcgga agtctcaagg gcctcgtcgg caaggtgcgc caatggatgg
     1081 cgttgcatcg taaggagcgt tggctggggc aggcgcaggc gcacgccgtg gagagcgctg
     1141 cgcagctcga ggccgggcgt cagcagatcg ccaccgacct cgccgcacgc aaggagcagg
     1201 cgggcgccgt ttcgacggcc actccacctg aggctgcagt gcccacttcc agcatgatgc
     1261 gcgccaccgc cggtgcccaa gcgaccggca ccattgcgcg gcctccgagc acgtccggcg
     1321 ccccaccggc cgcctccggc agcgcgctcg ccgccagaac gcgcgagatc gccgccgagc
     1381 aaagccgcct caccttacgc gggcagcgca tcacagcgcg caaacgcctc gccgagactt
     1441 acggccagtg gagcgcggtg gtcggaattc aggcgaacgc gctgctgcac tcggccctcg
     1501 cggacgtcgc agtagtgctc ggggcgctcg tgctgctgct cttcatcgac cgatggctgg
     1561 agacgctctt aggccgcgcc cccgtcgatc gccgccagat cgcaacgctg cggagcgtcg
     1621 tcggcgtctc ctgccagctg gtcggcatcg tcgtgattct cctcgtgctg atcggactgc
     1681 ccgggcagct cggcacgatg atcgggcttg cgggtgccgg gctcacggtg gcgctcaagg
     1741 acttcatcat cgggtttctc ggatggttcg tgctcatggg caagcacggc atgcgcgtcg
     1801 gtgactgggt cgagatcaac ggcgtgagcg gtgaagtggt cgagctcggc atcttccata
     1861 cggtgctcct cgagacgggc aactgggcgg atgccgggca tcccacgggc cgcagagtca
     1921 ctttcactaa tgccttcgcc atcgaaggcc attacttcaa cttctcgacg acgggtcagt
     1981 ggctctggga cgagctcctg gtcatggtgc ccttcgatcg ggatgcccgt gcgatcgcgg
     2041 atgcgatcca gaaggaggtg accgaggcga ccgcggagag ctcacgagag gccgaggccg
     2101 aatggcagcg cgctgcacgc gggcgcaagc tcggcttcac ggcacagccc ggcatcgcga
     2161 tccgccccgc accggggggc ggcggtgtcg agatcgcagt tcgctatgtc acacgcgcag
     2221 cggagcgctt tgcgctgcga tccaggctct accagtccgc cgtgcagctc ctgtcccagc
     2281 cgaagccggc ggcgtgaacc gtgagcgcac gacttccggc agtcttcctc gggcacggca
     2341 acccgatgaa tgccgtccgc gagaatgcct ggacgcgagc ctgggcggcg ttcggcgccg
     2401 cgctcgcccc gaagccgcgc gccgtgcttg cgatctcagc ccactggtac atcgtccgca
     2461 ctgcggtcac ggcgatggcg gctccgcgca ccatccatga ctttgggggc tttccgcgcg
     2521 agctcttcgc gatgcgctac ccggcccccg gtgatcccac gctcgccgtg cgtgtagccg
     2581 agctcctggc cccgctaccg gtcgtagcgg acgcatcgtg ggggctcgac cacggcacct
     2641 ggtccgtgct ctgccacgtg tttccggagg cg
//
LOCUS       DATLKL010000037         1658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_428, whole genome shotgun sequence.
ACCESSION   DATLKL010000037 DATLKL010000000
VERSION     DATLKL010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /gene="rpsM"
                     /locus_tag="VM713_00515"
     CDS             complement(<1..345)
                     /gene="rpsM"
                     /locus_tag="VM713_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HVH82774.1"
                     /translation="MARIAGINIPMNKHVWVGLTHIYGVGRSRAHAACEAAGVNPATK
                     VKDLTETEVNAIRSAVAKFAVEGDLRREVSMNIKRLMDLGSWRGIRHRRGLPVRGQRT
                     RTNARTRKGPRKQ"
     gene            complement(358..471)
                     /gene="rpmJ"
                     /locus_tag="VM713_00520"
     CDS             complement(358..471)
                     /gene="rpmJ"
                     /locus_tag="VM713_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HVH82775.1"
                     /translation="MKVRPSVKKICKNCKIVRRRRVVYVICSDPRHKQRQG"
     gene            complement(475..>1658)
                     /gene="secY"
                     /locus_tag="VM713_00525"
     CDS             complement(475..>1658)
                     /gene="secY"
                     /locus_tag="VM713_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HVH82776.1"
                     /translation="DKSNTILGIVNLFSGGALSRLSIFAMGVMPYISASIIIQMMSMV
                     VPSLMELRKEGESGRRKITEYTRYGTVILAAFQSFAAAGALAKGGMTTITGWPFLMIA
                     TITMTTGTMFLMWLGEQITERGIGNGISMIILSGIVAGLPGAVGNTMEAVSNGEMPGP
                     VALALLFLVVGVTAFVVFMERAQRRIPVDYAKRQVGRRMYAGQSTHLPFKINMSGVIP
                     PIFASSLLLFPATLASFFGANAEGPISGFMQDVAAALGYGQPLHLVIYAVLIIFFCFF
                     YTALVFNARDTAENLKKSGAFVRGIRPGQQTAEYIDQVLTRLTLWGALYVTAVCLVPE
                     VLISWYGVPFRFGGTSLLIVVVVVMDFMSQLNAHMMSHHYPGLLKKANLLGYGRTGPG
                     S"
BASE COUNT          290 a          540 c          552 g          276 t
ORIGIN      
        1 ctgcttgcgc ggtcccttgc gggtgcgcgc gttggtgcgc gtgcgctggc cgcgcaccgg
       61 aagcccccgg cgatggcgga tgccgcgcca ggaacccagg tccatcagcc gcttgatgtt
      121 catcgacact tcgcgcctca ggtcgccctc gaccgcgaac ttggcgacgg ccgagcggat
      181 cgcgttcacc tccgtctcgg tgagatcctt caccttggtg gccggattga cgcccgcggc
      241 ctcgcaggcg gcgtgcgcgc gcgagcggcc cacgccgtag atgtgcgtga gcccgaccca
      301 aacgtgtttg ttcatcggga tgttgatgcc ggctatgcgt gccatgtgtg acttacctca
      361 accctggcgc tgcttgtggc gcgggtccga gcagatgacg tagaccacgc gccggcggcg
      421 cacgatcttg cagttcttgc agatcttctt gaccgacgga cgtactttca ttgattaact
      481 acccggcccc gttcgcccgt agccgagcag gttggccttc ttgagaagtc ccgggtagtg
      541 atgcgacatc atgtgcgcgt tcagctgcga catgaagtcc atcaccacca ccacgacgat
      601 gaggagcgag gtgccgccga agcggaacgg cacgccgtac caggaaatga ggacctcggg
      661 caccaggcac accgccgtga cgtacagcgc gccccacagc gtcagccgtg tcagcacctg
      721 gtcgatgtac tccgcggtct gctgccccgg gcggatgccg cgcacgaagg cgccggactt
      781 cttcaggttc tcggcggtgt cgcgcgcgtt gaacaccagc gccgtgtaga agaagcagaa
      841 gaagatgatc agcaccgcgt agatcaccag gtgcagcggc tggccgtagc cgagcgccgc
      901 ggccacgtcc tgcatgaagc ccgagatcgg cccttcggcg ttggcgccga agaagctcgc
      961 gagcgtcgcc gggaagagca gcagactcga ggcgaagatc ggcggaatga cgcccgacat
     1021 gttgatcttg aacggcaggt gggtgctctg gccggcgtac atgcgccggc ccacctggcg
     1081 cttggcgtaa tcgaccggaa tgcgccgctg cgcgcgctcc atgaacacca cgaacgcggt
     1141 gacgcccacc accaggaaca gcagcgcgag cgccacgggg cccggcatct cgccgttgct
     1201 caccgcctcc atggtattgc ccaccgcgcc cgggagcccg gcgacgatgc cggagagaat
     1261 gatcatcgag atgccgttgc cgatgccgcg ctcggtgatc tgctcgccga gccacatgag
     1321 gaacatggtc cccgtggtca tcgtgatggt ggcgatcatc aggaacggcc agcccgtgat
     1381 cgtcgtcatg ccgcccttgg cgagcgcgcc ggccgcggcg aacgactgga aggcggcgag
     1441 gatcacggtg ccgtagcgcg tgtattcggt gatcttgcgg cggcccgact cgccctcctt
     1501 gcgcagctcc atgagcgagg gcaccaccat cgacatcatc tggatgatga tcgaggcgga
     1561 gatgtagggc atgacgccca tggcgaagat cgacaggcgc gacagcgccc cgcccgagaa
     1621 caggttgacg atcccgagga tggtgttgga cttgtcgc
//
LOCUS       DATLKL010000038         1861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_90673, whole genome shotgun sequence.
ACCESSION   DATLKL010000038 DATLKL010000000
VERSION     DATLKL010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90673"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..879
                     /locus_tag="VM713_00530"
     CDS             115..879
                     /locus_tag="VM713_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVH82777.1"
                     /translation="MGADNRIVAGRWWTREDAGKPLVSLATEFQESVGVNLGDRLTFD
                     IGGETIEAQVASVRKVKWDSFQPNFFIVFAPGVLERIAGTYMTSAYFRPGTAGTLAEL
                     AHRFPSVSIFDIDELLSQVRAVLDKAALAVQSVFVFTLFAGLTVLLAAVQSSRDERRY
                     ESAMLRTLGASRGTVVQGVLAEFVTLGTLSGLIAAAGASLAAYFLTTRWLELRYVFDL
                     VPWGVGIAGGALLVATGGWLATRSVVNQPPLTTLRA"
     gene            971..>1861
                     /locus_tag="VM713_00535"
     CDS             971..>1861
                     /locus_tag="VM713_00535"
                     /inference="COORDINATES: protein motif:HMM:NF019631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase
                     domain-containing protein"
                     /protein_id="HVH82778.1"
                     /translation="MLLERLRFTPTATTFAAVTAALNALLYHLPLFSFAAGTLDLSSG
                     TGELTLATLLVVVLSETALLLILLALVSERLLRPVCMLAALGNAVAVYFVATYHVILD
                     VTMMENVLNTDFRESAEYLHPTLLVYVLALGVLPCALLARVRIRRTPRLRLAAVALGC
                     LTVTVLWCLLAARSWLWFDENSKRVGGLIMPWSYAINTGRYLLPRLMASPAPLLLPPA
                     TFSSNEKTVVVLVIGEAARSKDFQLYGYGRPTNPLLSAAGVIALKGATACATYTTAAV
                     RCTLSRDAGRSVFSPPYEPLP"
BASE COUNT          259 a          639 c          614 g          349 t
ORIGIN      
        1 cattcttgaa ctgcggcgcg gtggcccagc caatggaatg gaacggagtg ggagcggggt
       61 agctaccatt gtggcttcgc gacgcgcgag cagaatctca cctggacgac ggaattgggg
      121 gccgacaacc gcatcgtcgc cggacgctgg tggacgcggg aggacgccgg caagccgctc
      181 gtatcgctcg ccaccgagtt ccaggagtcc gtcggcgtaa acctcggcga tcggctgacc
      241 ttcgatatcg gcggcgagac catcgaggcc caggtcgcga gcgtccgcaa ggtcaagtgg
      301 gacagcttcc agccgaattt tttcatcgtg ttcgcgccgg gggtgctgga gcgtatcgcg
      361 ggcacctaca tgacgagcgc ctacttcagg cccggcaccg cgggcacgct cgccgagctc
      421 gcgcaccgct tcccgagcgt ctcgatcttc gacatcgacg agctcctcag ccaggtacgc
      481 gccgtgctcg acaaggcggc gctcgccgtg cagagcgtat tcgtcttcac gctcttcgca
      541 gggctcaccg tgctgctggc ggcggtccag tcgagccgcg acgagcgccg ctacgagagt
      601 gccatgctgc gcacgctcgg cgcgagccgc ggcaccgtgg tgcagggtgt gctggcggag
      661 ttcgtgacgc tcggaacgct gtcggggctg atcgccgcag ccggcgcctc gctcgccgcc
      721 tacttcctca cgacccgctg gctcgagctg agatacgtgt tcgatctcgt gccatggggc
      781 gtcggcatcg ccggtggagc gctcctcgtg gcgaccgggg gctggctcgc cacgcgcagt
      841 gtggtgaatc agccgccgct cacgacgcta cgggcttgaa ggcgcgtggg acgatggcgg
      901 ggttcgccgt gtcccggtga cctgagcccc tggtcgtgcg cacccggcag cggcaatact
      961 tgtcccgccc atgctgcttg agcgactgcg cttcacgccc accgcgacga ccttcgccgc
     1021 cgtaacggcg gcgctcaatg ccctgctgta tcacctgccg ctcttctcct tcgccgcggg
     1081 cacgctcgac ctgtcttccg gcaccgggga gctgacgctc gccaccttgc tggtggtcgt
     1141 cttgtccgag accgctctgt tgctcatcct gctggcgctc gtctcggaac ggctgctgcg
     1201 gcccgtctgc atgttggcgg cgctcggcaa tgcggtcgcc gtgtacttcg tcgccaccta
     1261 tcacgtgatc ctggacgtca cgatgatgga gaacgtcctc aacaccgatt tcagggagtc
     1321 cgccgaatac ctgcatccga cgctcctcgt ctatgtgctg gcgctcggcg ttctgccctg
     1381 cgctctgctc gcgcgcgttc gcatccggcg gacgccgcgc ctgcggctgg cggccgtggc
     1441 gctcggctgc ctcacggtca ccgtcctctg gtgcctgctg gcagcgcgca gctggctctg
     1501 gttcgatgaa aactcgaaga gggtgggcgg cctgatcatg ccgtggtcgt acgccatcaa
     1561 caccgggaga tatctgctgc cgaggctgat ggcatcgcca gccccgctgc tgctgccgcc
     1621 cgccaccttc tcttccaatg agaagaccgt cgtcgtactg gtgattggcg aagccgcgcg
     1681 ctcgaaggat ttccagctct atggctacgg ccgtccgacg aatcccttgc tgtcggcggc
     1741 cggcgtcatc gccctgaagg gtgcgacggc ctgtgccacc tacacgacgg cggccgtgcg
     1801 ctgcacgctc tcgagggacg cgggcagatc agtgttctcc ccgccgtacg agccactgcc
     1861 t
//
LOCUS       DATLKL010000039         2493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_203236, whole genome shotgun sequence.
ACCESSION   DATLKL010000039 DATLKL010000000
VERSION     DATLKL010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203236"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VM713_00540"
     CDS             <1..452
                     /locus_tag="VM713_00540"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HVH82779.1"
                     /translation="SLSPNYQYHTSGGEPTITGYTALNVVEVTLDDLARIGDVIDAAT
                     RAGANRVQGIQFTVRDQDAVRAQALREAATRAHAEAEVLAGALNLRILRVLSVEESSP
                     QIVPVRPRLAVARAAASTAEVPTPVEAGTLDVSANVALTVEVGPAAH"
     gene            488..916
                     /locus_tag="VM713_00545"
     CDS             488..916
                     /locus_tag="VM713_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008050583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVH82780.1"
                     /translation="MTTVQTLLEQKGRAIYSVEPRAAVLDAIRLMAEHHVGALVVMRG
                     ATLEGIVSERDYALKVILRGRSHTDTVVRDIMTSPVLTVSLDATVQQCMQLVTDRRVR
                     HLPVVDGGRVVGMVSIGDLVKSVIAEQQQQIEQLESYIHS"
     gene            complement(966..2414)
                     /locus_tag="VM713_00550"
     CDS             complement(966..2414)
                     /locus_tag="VM713_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HVH82781.1"
                     /translation="MLAVAYVLGLLLAAFALTFALPIACSLLMGDGLWPQFLLTAAIA
                     AGCGLVLAAGTRRLKRELRSRDGFLLVTLGWLMLSAAAAVPLLLILPTLSFTGAYFEA
                     VSGLTTTGSTVLTGLDALPPSLNFWRASLHWFGGLAIIVMALAVLPLLGVGGMQLYKG
                     QAPGPAKEERLTPRITETARSLWLVYALITAAGIVALRICGMSWFDAVCHAFSAVGLG
                     GFSTHDRSIGYFDSPAIELTLVALMLVASLNFARHFIALRRLSLDTYRVDPECRAIFL
                     ILAVSVVGIAALLSWHGVYPSLMTALRHSAFNVVSQATTSGLTSQDFQQWPVFAPYWM
                     LFLSCIVCSTGSTGGGIRMFRTLLLARQAGRELKLLVHPAALAPVRVGGRPIPDSVGH
                     AVLAFIFGYFMTVVFFTFALLLTGLDFDSALAASVASINNTAHGFGPPGAVHNFHGLT
                     VAQTWLCTAAMLLGRLEIFSVIVLATPAYWRK"
BASE COUNT          444 a          827 c          915 g          307 t
ORIGIN      
        1 actccctgag ccccaactat cagtaccaca cctcgggcgg cgagccgacc atcaccggct
       61 acacggcgct gaatgtcgtc gaggtgacgc tcgatgatct cgcgcgcatc ggcgacgtga
      121 tcgacgcggc cacccgcgcg ggcgccaatc gggtgcaggg catccagttc acggtgcgcg
      181 accaggacgc ggtgcgcgcc caggcgctgc gcgaggcggc gacccgcgcc cacgccgagg
      241 cggaggtgct cgcgggggcg ctgaatctca ggatcctgcg cgtgctcagc gtcgaggaat
      301 cgagcccgca gatcgtgccc gtgcggcccc gcctggcggt ggcgcgcgcc gcggcgagca
      361 ccgcggaagt accgacgccg gtcgaggcgg gcacgctcga cgtgagcgcc aacgtcgctt
      421 tgacggtgga ggttggcccg gccgcgcatt aggcggcccg cctgccgctc tttccggagg
      481 tgaggacatg acgacggtgc agacgctgct cgaacagaag ggccgggcga tctattcggt
      541 ggagccgagg gcggcggtgc tcgatgcgat ccgcctgatg gccgagcatc acgtgggggc
      601 gctggtcgtc atgcgcggcg cgaccctcga aggcatcgtc tccgagcgcg actacgcgct
      661 caaggtgatc ctgcgcggcc gctcccacac cgacacggtg gtgcgcgaca tcatgacctc
      721 cccggtgctc acggtatccc tcgacgccac ggtgcagcag tgcatgcagc tcgtgaccga
      781 ccgccgtgtg cgccacctgc cggtcgtcga cggcgggcgc gtcgtcggca tggtgtcaat
      841 cggcgacctg gtgaagtccg tcatcgccga gcagcagcag cagatcgagc agctcgagag
      901 ctacatccac agctgagcgc gggcgtaggt cagcggactg cgcccggcag gagcgcagag
      961 accggttact tgcgccagta ggcgggcgtc gcgagcacga tcacgctgaa gatctcgagc
     1021 cgcccgagca gcatcgccgc cgtgcacagc caggtctgcg ccacggtgag cccgtggaag
     1081 ttgtgcaccg ccccgggcgg accgaagccg tgggcggtgt tgttgatcga ggcgacgctc
     1141 gcggcgagcg ccgagtcgaa gtcgagcccg gtgagcagca gcgcgaaggt gaagaacacc
     1201 accgtcatga agtagccgaa gatgaaggcg agcaccgcgt ggcccaccga gtcggggatc
     1261 ggacgtccgc cgacgcgcac cggcgccagg gcggccggat ggaccaggag cttcagctcc
     1321 cggcccgcct ggcgcgcgag cagcagggtg cggaacatcc tgatgccgcc gccggtcgag
     1381 ccggtgctgc agacgatgca cgagaggaac agcatccagt aaggcgcgaa taccggccac
     1441 tgctggaagt cctggctggt gagcccgctg gtggtcgcct gcgagaccac gttgaaggcg
     1501 gagtgacgca gcgccgtcat caagctcggg tagaccccgt gccagctgag gagcgcggcg
     1561 atgccgacga cgctcaccgc gaggatcagg aagatcgcgc ggcactccgg atcgacccga
     1621 taggtgtcga ggctcaagcg ccggagcgcg atgaaatggc gcgcgaagtt gagcgaggcg
     1681 accagcatga gcgcgaccag cgtcagctcg atcgccgggg aatcgaaata gccgatgctg
     1741 cggtcgtggg tcgagaagcc gccgaggccg acggcggaga aggcatggca gaccgcatcg
     1801 aaccagctca tgccgcagat ccgaagcgcc acgatgccgg ccgcggtgat gagcgcgtag
     1861 acgagccaca gcgagcgcgc cgtctcggtg atgcgtggcg tcagccgctc ttccttggcc
     1921 ggccccggcg cctggccctt gtagagctgc atgccgccga cgccgagcag cggcagcacc
     1981 gcgagcgcca tgacgatgat cgcgagccca ccgaaccagt ggagcgaggc gcgccagaag
     2041 ttgagcgagg gcggcagcgc atcgagcccg gtgagcacgg tcgagcccgt agtggtgagt
     2101 ccggacaccg cctcgaagta cgcgcccgtg aagcttaggg tgggcaggat gagcagcagc
     2161 ggcaccgccg ccgcggccga gagcatgagc cacccgagcg tcacgagcag aaagccgtcg
     2221 cgcgagcgca gctcgcgctt caggcggcgt gtgcccgcgg cgagcaccag tccgcacccc
     2281 gcggcgattg ccgcggtcag caggaactgc ggccacagcc catctcccat gaggagcgag
     2341 caggcgatcg gcagcgcgaa agtcagcgcg aaggccgcga gcaggagccc gagcacgtag
     2401 gcgacggcga gcacggcgca gcgtccgccc tgagcgtcag cgcgccgagc gcaggaagag
     2461 cttctcgacc gcctcgaggt ggcgtcgatc ggc
//
LOCUS       DATLKL010000040         1558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_68130, whole genome shotgun sequence.
ACCESSION   DATLKL010000040 DATLKL010000000
VERSION     DATLKL010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68130"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /gene="rpmD"
                     /locus_tag="VM713_00555"
     CDS             <1..186
                     /gene="rpmD"
                     /locus_tag="VM713_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HVH82782.1"
                     /translation="KTVKVTQIGSPIGREKSHRATLIGLGLNKMHRTVELEDTPAVRG
                     MINRVRHLLHIEGEDRA"
     gene            241..747
                     /gene="rplO"
                     /locus_tag="VM713_00560"
     CDS             241..747
                     /gene="rplO"
                     /locus_tag="VM713_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HVH82783.1"
                     /translation="MKLNQLSDRPGARKRGKRIGRGIGSGKGKTAGRGGKGQTARSGV
                     SLLGFEGGQMPLFRRLPKRGFRNPFKKRFSLVNLGYLQQAIEAGRLDASATVDEKALA
                     AAGLIRSARYGVRLLAKGALKSKITIHVAGASKAAIAAVEKAGGKVVVTAQSAAATSG
                     PASTATGQ"
     gene            782..>1558
                     /locus_tag="VM713_00565"
     CDS             782..>1558
                     /locus_tag="VM713_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HVH82784.1"
                     /translation="MASAAEQLAANINFGAFAKATELKKRIWFTLGALIIYRLGTYIP
                     VPGIDPTILQDVFTQNSRGILGMFDMFAGGALRRMSMFALNVMPYISASIIMQLLTAI
                     SPTIEALKKEGEQGRKKINQYTRYGTVILAAFQAYGLAIGLEGMSGSHGPAVADPGLF
                     FRITTVFSLTAGTLFLMWLGEQVTARGVGNGVSLIIFSGIVANLPTALANTLELGRTG
                     SLSTFVLIVLLAMAVAVIAAIVFMERAQRRIVVQYPKRQTG"
BASE COUNT          275 a          533 c          499 g          251 t
ORIGIN      
        1 aagacggtta aggtaaccca gatcggcagc ccgatcggcc gcgaaaagtc ccatcgggcg
       61 accctgatcg gcctcggcct caacaagatg catcgcacgg tcgagctcga ggataccccg
      121 gcagtgcgcg gcatgatcaa ccgcgtccgc catctcctgc acatcgaggg cgaggatcgt
      181 gcctagtcga ggacgaggcg cggcgcggct gggacctcat cgagtccaag gaccaagacc
      241 atgaaactga accagctttc cgaccgtccg ggtgctcgca agcggggcaa gcgcatcggc
      301 cgcgggatcg gctcaggcaa gggcaagacc gcggggcgcg gcggcaaggg ccagaccgcg
      361 cggagcggcg tgtccctgct cggctttgag ggcggccaga tgccgctatt ccggcgtctg
      421 cccaagcggg gcttccgcaa cccgttcaaa aagcgcttca gcctggtcaa tctgggctac
      481 cttcaacagg caatcgaggc cggccggctc gacgccagcg cgacggtcga tgagaaggcc
      541 ctggcggcgg ccgggctcat ccggtccgcc cggtacggag tccggctgct tgcaaagggc
      601 gcgctcaagt ccaagattac cattcatgtc gccggcgcct ccaaggccgc gattgcggcg
      661 gttgagaagg ccggcggcaa ggtggtcgtg accgcccagt cggcggccgc gacgtctggg
      721 cccgccagca ccgccaccgg gcagtaaccc tcgagggctt gggccctcgc cggagagcat
      781 catggcttca gccgccgaac agctcgccgc caacatcaat ttcggcgcct tcgccaaggc
      841 gaccgagctc aagaagcgca tctggttcac cctcggcgcg ctgatcatct accggctcgg
      901 cacctacatc ccggtgcccg gcatcgaccc caccattctg caggacgtgt tcacccagaa
      961 ctcgcgcggc atcctcggca tgttcgacat gttcgccggt ggcgccttgc gacggatgag
     1021 catgtttgcc ctcaacgtga tgccgtacat ttcggcatcg atcatcatgc agctgctcac
     1081 cgccatctcg cccacgatcg aggcgctcaa gaaggagggc gagcagggcc ggaagaagat
     1141 caaccagtac acccggtacg gcaccgtcat cctcgccgcc ttccaggcct acggcttggc
     1201 tattgggctc gagggcatga gcggcagcca tgggccggca gtcgccgatc ccggcctgtt
     1261 cttccggatc accacggtat tttcgctcac cgccggcacc ctcttcctga tgtggctcgg
     1321 cgagcaggtg acggcgcgcg gggtaggcaa cggcgtgtcg ctgatcatct tctcgggcat
     1381 cgtcgccaac ttgccgacgg cactcgccaa cacgctcgag ctcgggcgca cagggtcgct
     1441 ctccaccttc gtcctcatcg tgctgctcgc gatggcggtg gccgtgatcg cggcgatcgt
     1501 gttcatggag cgcgcgcagc ggcgcatcgt cgtccaatac ccgaagcggc agacaggc
//
LOCUS       DATLKL010000041         1580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_11671, whole genome shotgun sequence.
ACCESSION   DATLKL010000041 DATLKL010000000
VERSION     DATLKL010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11671"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VM713_00570"
     CDS             complement(<1..912)
                     /locus_tag="VM713_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HVH82785.1"
                     /translation="MRADSRVYIAGHRGLVGSAIRRCCETRGFTRLILRGRDELDLRD
                     GQAVGRFFAAERPEYVFLAAGRVGGIHANDSRPADFIRDNLQIETNVIDAAWRYGARK
                     LLFLGSSCIYPRLAPQPLREEYPLTWALEPTNEWYAIAKLAGLKMCQAYRRQHGFDAI
                     TLLPTNLYGPGDNFDPDTSHVLPALLRKIDAAQRARAGRVTLWGSGTPRREFLFVDDL
                     ADACLFLMERYTDERPINVGSGEELTIRELAGLIAEVVGFRGGFELDPSRPDGTPRKV
                     LDVSRLAALGWKPRVDLRTGIGLTCDWY"
     gene            complement(915..>1580)
                     /locus_tag="VM713_00575"
     CDS             complement(915..>1580)
                     /locus_tag="VM713_00575"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HVH82786.1"
                     /translation="EIPQSERTPFHPRSPYGVAKLYAHWITVNYREAYGLYACSGILF
                     NHESPLRGETFVSRKITRGLARVSVGLQGCVHLGNLEARRDWGHARDYVEAQWLMLQQ
                     QHPVDYVIATGRQHSVRELVEKSAALLDMRLEWRGRGAEEVGVNRKTGQAIVRIDPRY
                     FRPAEVDALLGDASKARGELGWAPRVTFDELVREMVESDLALAERDALVTREGYRLAQ
                     ARD"
BASE COUNT          249 a          529 c          565 g          237 t
ORIGIN      
        1 gtaccagtcg caggtgagcc cgatcccggt gcgcagatcg acgcgcggct tccagccgag
       61 ggcagcgagc cgcgacacgt ccagcacctt gcgcggcgtg ccgtcggggc gcgaagggtc
      121 gagctcgaag ccgccgcgga aaccgaccac ctcggcgatg agcccggcga gctcgcggat
      181 cgtgagctcc tcgccgctac cgacattgat cggccgctcg tccgtatagc gttccatcag
      241 gaagagacag gcatcggcga gatcgtcgac aaagaggaac tcgcgccgcg gcgtcccgct
      301 gccccacagc gtgactcgcc cggctcgcgc gcgttgtgcg gcgtcgatct tgcgcaacaa
      361 cgccggcagc acgtgcgagg tgtccgggtc gaagttgtct ccgggaccgt atagattggt
      421 cggcagcagc gtgatggcgt cgaagccgtg ctggcggcgg taggcctggc acatcttgag
      481 ccccgcgagc ttggcgatcg cataccactc gttggtcggc tccagtgccc aggtgagcgg
      541 gtactcctcg cgcagcggct gcggggcgag cctcgggtag atgcagctcg agccgaggaa
      601 caggagcttg cgcgccccgt agcgccaggc ggcatcgatg acgttggtct cgatctgcag
      661 gttgtcgcgg atgaagtccg ccgggcgcga atcgttggcg tggatcccgc cgacgcgccc
      721 ggcggcgagg aacacgtact cgggacgctc ggcagcgaag aagcgcccca ccgcctgccc
      781 gtcgcgcagg tcgagctcgt cccggccgcg gaggatcagc cgggtgaagc cacgtgtctc
      841 gcagcagcgg cggatggcgg agccgaccag cccgcggtgg cccgcaatgt agacgcggct
      901 gtcggcgcgc atcgctaatc gcgcgcctgg gcgaggcggt agccctcgcg ggtaacgagc
      961 gcatcgcgct cggccagggc caggtccgat tcgaccatct cgcgcaccag ctcgtcgaac
     1021 gtcacccggg gggcccagcc gagctcgccg cgcgccttgg aggcgtcccc gagaagcgca
     1081 tcaacttcgg cggggcggaa atagcgcggg tcgatgcgga cgatcgcctg gccggtcttg
     1141 cgattgaccc caacctcctc ggctccgcgg ccgcgccatt cgagccgcat gtcgaggagc
     1201 gccgcgctct tctcgacgag ctcgcgcacc gagtgctgcc gccccgtagc gatcacgtag
     1261 tccacggggt gctgctgctg cagcatcagc cactgtgctt cgacgtagtc gcgtgcgtgt
     1321 ccccagtcgc gccgtgcctc gaggttgccg agatggacgc agccctgcag cccgacactg
     1381 acgcgcgcga gcccccgggt gatcttgcgc gagacaaaag tctccccccg cagcggcgac
     1441 tcgtgattga agagaatgcc gctgcaggcg tagaggccgt aggcctcgcg atagttgaca
     1501 gtgatccagt gggcgtagag cttggcgacg ccgtagggag agcgcgggtg gaagggagtg
     1561 cgctcgctct gcgggatctc
//
LOCUS       DATLKL010000042         2586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_214572, whole genome shotgun sequence.
ACCESSION   DATLKL010000042 DATLKL010000000
VERSION     DATLKL010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214572"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VM713_00580"
     CDS             complement(<1..832)
                     /locus_tag="VM713_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVH82787.1"
                     /translation="MTDAEHTVRIEPHGRTLRVRRDEPVLQAALAAGLNLPHSCKSGH
                     CSSCRARLVAGEIHYPNGRPPGVTAAEAGSGQVLLCQARPRSDLVVEARLIASVADVE
                     IKTLPCRIERLTALAPDVLQVMLRLPAVERLRFHPGQYLDVLLEGAKRRSFSIACPPH
                     DAQLIELHVRRVPGGGFTERLFAPPALSRPGDQALAGSTSPAQALAGSTSHEQALAGS
                     TSPAQALALGSLLRIEGPMGQFSYRAGESPILMVGGGTGFAPLKSMLRHVLETGIRRD
                     I"
     gene            1050..1877
                     /gene="dapF"
                     /locus_tag="VM713_00585"
     CDS             1050..1877
                     /gene="dapF"
                     /locus_tag="VM713_00585"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009565969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HVH82788.1"
                     /translation="MRIAFTKMHGVGNDFVVFDAPPAAVLEAASLKRLADRRTGIGFD
                     QALSLEAPRRAGSAVFYRVFNADGDEVEQCGNGARCIAALLHSRGQTRAGRVTLESPA
                     GVVEAHVSGAANVTVDMGVPNFDPRSLPFDAPVEIDPYPLEIGGRTLEIGAVSLGNPH
                     AVLVVPSVEAAPVESLGPAIERHARFPRRVNAGFLEIVDRAHLKLRVYERGAGETLAC
                     GTGACAAVAVARRRELIDSEVRLEVRGGELRVNWAHPGEHIWLSGPAQVSFEGHVEV"
     gene            1892..>2586
                     /locus_tag="VM713_00590"
     CDS             1892..>2586
                     /locus_tag="VM713_00590"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HVH82789.1"
                     /translation="MTTREARGLTAVESEEESIATYLQRNPDFFERHQGVLVRLKLPH
                     ARGGAAISLVERQIEVLREKLAGVEGKLAELLRTARANDAIADRLHRFTRRLLRAVPR
                     AEAIARIEAGLREDFDAFHSALVLIGEQPGLPPQQFVRVVAPDDPNLKSFESLLASGK
                     PRCGQARDSQREFLFGPEGPEMGSLALVPLGHKGSIGLLALGSVDRDRFHPGMSTEFL
                     ARMADLIADALTAT"
BASE COUNT          381 a          890 c          920 g          395 t
ORIGIN      
        1 ggatgtcgcg ccggatgccg gtctcgagca cgtggcgcag catcgacttg agcggcgcga
       61 aaccggtgcc gccccccacc atgaggatgg gcgattctcc ggcgcggtag ctgaattgtc
      121 ccatcggccc ctcgatgcgc agtagcgacc cgagcgccag cgcctgagca ggcgaggttg
      181 accccgccag cgcttgttca tgcgaggttg accccgccag cgcctgagca ggcgaggttg
      241 accccgccag cgcctgatca cccgggcggg acagcgcggg cggcgcgaac aggcgctcag
      301 tgaagccgcc gcccggcacg cgccgcacgt gcagctcgat gagctgcgcg tcgtggggtg
      361 gacaggcgat cgagaagctg cggcgcttgg ccccttcgag cagtacatcg agatattggc
      421 cggggtgaaa gcgcagccgc tccacggccg gcagccgcag catgacctgc aggacgtcgg
      481 gagccagcgc tgtcaaacgc tcgatccggc acggcagcgt cttgatctcg acatccgcca
      541 cgctcgcgat gaggcgggcc tcgacgacga gatccgagcg cggccgtgcc tggcagagca
      601 gcacttggcc gctcccggcc tctgccgccg tgacgccggg tggccggccg ttcgggtaat
      661 gaatctctcc ggcgaccagc cgcgcgcggc acgagctgca atggccggac ttgcagctgt
      721 gcgggagatt gagcccggcg gcgagcgccg cctgcagcac gggctcgtcg cggcgcaccc
      781 ggagcgtgcg cccgtgcggc tcgatgcgta cggtatgttc ggcgtcggtc aagttcgctc
      841 cgggcctgtg actgcaacat gcgcgttgcg cagggtgaat agactagacg cgccgcccgg
      901 caggggccaa ggagatgcct gtgtttgtga agcgcgaccg tgccgccatg ctttattgaa
      961 aaaccacctg cggtgatttt tgtgccgacc ccgggccggc accctgcgga ctgctcagtg
     1021 ccaccgcgtt ttgcgttatc ctgcgcccga tgcgcatcgc tttcacgaag atgcacggag
     1081 tcggcaatga cttcgtcgtg ttcgatgcgc cccccgccgc agtgctcgag gccgcgagcc
     1141 tcaagcggct cgccgaccgc cgtactggca tcggcttcga tcaggcgctc tctctcgagg
     1201 caccgcggcg cgccggcagc gccgtcttct accgggtctt caacgccgac ggcgatgagg
     1261 tcgagcagtg cggcaacggc gcgcgctgta tcgcggcgct cctgcacagc cgtggccaga
     1321 cgcgcgcggg ccgcgtgacg ctcgagagcc cggccggggt ggtcgaggcg cacgtgagcg
     1381 gcgcggccaa tgttaccgtc gacatgggtg ttcccaactt cgacccgcgc tcgctgccct
     1441 tcgacgcgcc cgtggagatc gacccctatc cgctcgagat cggcggccgt acgctcgaga
     1501 tcggcgcggt ctcactcggc aatccgcacg cggtgctcgt ggtcccctcg gtcgaggcgg
     1561 cgccggtgga gtcgctcgga ccggcgatcg agagacacgc gcgcttcccg cggcgtgtga
     1621 acgccgggtt cctcgagatc gtcgaccgtg cgcacctcaa gctgcgcgtc tacgagcgcg
     1681 gcgccggcga gacgctcgcc tgtggcacgg gcgcgtgtgc cgcggtcgcg gtggcgcggc
     1741 gacgggagct gatcgactcc gaagtgcgcc tcgaggtgcg cggcggcgag ttgcgcgtaa
     1801 actgggcgca cccgggagag cacatctggc tgagcggtcc ggcgcaagtc tcattcgaag
     1861 gtcacgtcga ggtctagaga ggtccgatgt catgacaaca agagaagctc ggggtctcac
     1921 cgcggtcgag tcggaagagg agtcgatcgc cacctacctg cagcgcaacc cggatttttt
     1981 cgagcggcat cagggggtgc tggtgcgcct caagctcccg cacgcgcgcg gcggcgccgc
     2041 catctcgctc gtcgagcgtc agatcgaggt gctgcgcgag aagctcgccg gggtggaggg
     2101 caagctcgcc gagctgctgc gtaccgcgcg cgccaacgac gcgatcgccg accgcctgca
     2161 tcgcttcacg cggcggctgc tgcgcgccgt accgcgcgcc gaggcgatcg cgcgcatcga
     2221 ggcgggactg cgcgaggact tcgacgcctt ccattcggcg ctggtgctga tcggcgagca
     2281 gccgggcctg ccgccgcagc agttcgtgcg cgtcgtagcg ccggacgatc ccaacctcaa
     2341 atccttcgag agcctgctcg cgagcggcaa gccgcgctgc ggccaggcgc gcgactcgca
     2401 gcgcgagttc ctgttcggcc ccgagggccc ggagatgggc tcgctggcac tcgtgccgct
     2461 cggccacaag ggctcgatcg ggctgctggc gctcggcagc gtcgaccgcg accgcttcca
     2521 cccggggatg agcaccgagt tcctcgcccg catggcggac ctgatcgccg atgcgctgac
     2581 cgcgac
//
LOCUS       DATLKL010000043         1695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_56856, whole genome shotgun sequence.
ACCESSION   DATLKL010000043 DATLKL010000000
VERSION     DATLKL010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56856"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1695)
                     /locus_tag="VM713_00595"
     CDS             complement(<1..>1695)
                     /locus_tag="VM713_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH82790.1"
                     /translation="HRNALRLLNLVNALLDFARIEAGRVQAHYQPTNLSELTAELASS
                     FDSATERARLTLTIDCPPLPQAVYVDREMWEKIVLNLISNAFKHTLEGGILVRLEWVD
                     GGAQLTVADSGVGIPAEELPRLFERFHRVEGAPSRAHEGSGIGLSLVRELVQLHSGAI
                     RVESELGKGSRFIVSLKAGMAHLPADQIAVSGDDDSVGTRTTAALAQQALSWVPDGSE
                     SDPGVVEALPEHLGPRPRILLADDNPDMRRYIARLLRRSYDVEVAADGEAALAAACAN
                     PPNLVLSDVMMPRLDGFGLLRALRADERTRQLPVILVSARAGDEAAIEGLDAGADDYL
                     VKPFSARELLARIRAHLELARQRRELEQELERRVAARTADVARLTRVLQMLSGINSAL
                     VRIADRKDVMAEACRLARSVGGYSLSMVALIDPATRMARPVAWSGYEFLAHPEREFPV
                     ADHEAKDSSLMGRVIRTGQATLCEDIETFPHVIHSRRELVNAGVRSLACLPLRVDGTP
                     VGAFLFGTRHGELIHADEMLLLEEVAANLSFALQYLDERDTVHFLSYFEPLTGLAK"
BASE COUNT          273 a          558 c          591 g          273 t
ORIGIN      
        1 gtttggcgag gcccgtcagc ggctcgaaat acgagaggaa gtgaactgtg tcccgctcgt
       61 ccagatactg cagtgcgaac gatagattgg cggcaacctc ctcgaggagc agcatctcat
      121 cggcgtggat gagctccccg tggcgagtcc cgaagaggaa ggcgccgacg ggtgtgccgt
      181 cgacccgcag cggcaggcac gcaagggagc gtactccggc gttcaccagc tcccgacggc
      241 tgtggatgac gtgagggaag gtctcgatgt cttcgcagag cgtcgcctga cccgtgcgga
      301 tcacccgtcc catgaggctt gaatccttgg cctcgtgatc ggccacgggg aattccctct
      361 cggggtgggc gaggaactcg tagcccgacc aggcgacggg gcgtgccatg cgggtcgcag
      421 ggtcgatcag cgccaccatg gatagcgaat agccgcccac gctgcgcgcc aggcggcagg
      481 cttcagccat gacgtccttg cggtccgcaa tccgcaccaa tgccgagttg atccccgaga
      541 gcatctgcag gacccgggtc agacgcgcta cgtccgcggt gcgcgccgcg acccgccgtt
      601 ccagctcctg ttccagctcc cggcgctgcc gcgcgagctc gaggtgtgcg cgtatccggg
      661 cgagaagctc acgcgcggag aacggcttga ccaggtagtc gtcggcgccg gcatccaggc
      721 cttcgatcgc ggcctcgtca ccagctcgtg ccgacaccag aatgacggga agctggcggg
      781 tgcgctcgtc ggcgcgcagc gccctgagga gcccgaagcc gtcgagccgc ggcatcatca
      841 cgtcggacag cacgagattc ggcgggttgg cgcacgcggc tgccagggcc gcctcgccgt
      901 cggcggccac ttccacatca tatgaccgac gcaggagccg cgcgatgtag cggcgcatgt
      961 cggggttgtc gtcggcgagc aggatgcgtg ggcgcgggcc gaggtgctcg ggcaacgcct
     1021 ccacgacacc ggggtctgat tctgatccgt cgggcaccca ggacagcgcc tgctgcgcca
     1081 gcgctgcggt cgtgcgggtt cccacactgt cgtcgtcgcc gctcacggca atctgatctg
     1141 ccgggagatg ggccatgccg gccttgaggc tgacgatgaa gcggcttcct ttgccgagct
     1201 cgctctcgac ccggatggcc cccgagtgga gctgcaccag ctcgcgcacc agcgacaacc
     1261 cgataccgct gccttcgtgg gcgcgcgacg gcgccccctc gacgcggtga aagcgctcga
     1321 agagccgcgg cagctcctcg gccgggatcc cgaccccgct atcggccacg gtgagctgcg
     1381 cgccgccatc gacccattcg aggcgcacca ggatgccgcc ctcgagggtg tgcttgaagg
     1441 cgttcgagat gaggttcagt acgatctttt cccacatctc gcggtcgaca tacactgctt
     1501 gtggcagggg cgggcagtcg atcgtgagtg tgagtcgcgc gcgctcggtc gccgaatcga
     1561 agctgctggc gagctcggcg gtgagctcgc tgaggttcgt cggctgataa tgcgcctgca
     1621 cgcgtcccgc ctcgatgcgc gcgaagtcga gcaaggcatt gaccaggttg agcaggcgca
     1681 gggcattgcg gtgag
//
LOCUS       DATLKL010000044         1628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_45467, whole genome shotgun sequence.
ACCESSION   DATLKL010000044 DATLKL010000000
VERSION     DATLKL010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45467"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..796)
                     /locus_tag="VM713_00600"
     CDS             complement(26..796)
                     /locus_tag="VM713_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82791.1"
                     /translation="MPTPRSGAALVLACLLVTAPHLATAQTDEIQVYNAEITAPGHWN
                     LTWHNNFTPSGRATPDFPGGIVPNHALNGVPEWALGLTDWFEGGLYLPVYTRTPDGAL
                     LFDSAKLRALFVVPDAHDRSFFYGLNFELSYNTPHWEPSRFSGEIRPIIGWHLGRCDL
                     IVNPILDTDFNGLARLDFAPAVRAAYNFNRKMALALEEYADYGPLDHLRSGPNQQHTL
                     FAVLDLGSSTNGLEFGIGRGLTHASDSTVLKLMLMHDL"
     gene            complement(816..>1628)
                     /locus_tag="VM713_00605"
     CDS             complement(816..>1628)
                     /locus_tag="VM713_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH82792.1"
                     /translation="DLAGRSADDLSPVRTAEMPVETRALGVALNGLLARHAEVLARER
                     RFTADAAHELRTPLAALRAQAQVAARAATPAEARRALEKLQAGIDRTTHLMSQLLSLA
                     RLEPGSAPEAGQTTVASTVVDRVVDDLAHAASEKSVSVSVSASLQLLPGSPEVLYLLL
                     RNLLENSIRHVGERGHVALEVTRAGTSVVLSVSDDGPGIPSAERSRAFERFYRIPGSG
                     SGGSGLGLSIVGRIVELLSGTVELSAPPGGTGLIVRICLPLARTAAPDRSAA"
BASE COUNT          275 a          519 c          574 g          260 t
ORIGIN      
        1 tcgcggagtg atttggttcg cgcggctaaa ggtcatgcat cagcatgagc ttgagtaccg
       61 tggaatcgct ggcgtgcgtc agtcccctgc cgattccgaa ctcgaggccg ttggtgctcg
      121 agcctaagtc gagcaccgcg aacagcgtgt gctgctgatt cggaccggac ctgagatggt
      181 cgagcggtcc gtagtcggcg tattcctcga gtgcgagcgc catcttccga ttgaagttgt
      241 aggcggcgcg caccgcgggt gcgaagtcca gcctggcgag gccgttgaag tccgtatcga
      301 ggatcgggtt cacgatgagg tcacagcgac cgaggtgcca gccgatgatg ggacggatct
      361 cacccgagaa tcggctcggc tcccagtgag gagtgttgta gctcaactcg aaattcagcc
      421 cgtagaagaa gcttcgatcg tgcgcgtcgg gcacgacgaa gagtgctcgg agcttcgcgc
      481 tgtcgaacag cagcgcgcca tccggagtgc gcgtgtagac cggcagatag agcccgcctt
      541 cgaaccagtc ggtaagcccc aaggcccact cgggcacgcc gttgagcgcg tggttcggca
      601 cgatgccgcc ggggaaatcc ggcgtcgcgc gccccgacgg cgtgaaattg ttgtgccagg
      661 tcaggttcca gtggccgggc gccgtgatct cggcattgta gacctggatc tcgtcagtct
      721 gagcggtggc gaggtggggt gcagtcacca gcaggcaggc gagcaccaga gccgcgccgg
      781 agcgcggagt cggcatcgat gctggcatcg gacgtctagg cggcgctccg atcgggcgcc
      841 gccgttcgtg cgagcggcag gcagatcctc acgatgaggc ccgtccctcc cggcggcgcc
      901 gacagctcga ccgtgcccga gagcagctcg acgattcgcc cgacgatcga gaggccgagc
      961 ccgctgccgc cggaaccgct cccgggaatg cggtagaagc gttcgaaggc gcgcgagcgc
     1021 tcggcgctcg ggatcccggg cccgtcgtca ctcaccgaaa gcaccactga ggtgccggca
     1081 cgcgtcacct cgagcgcgac gtgaccgcgt tcgccgacgt gacggatcga gttctcgagg
     1141 agattacgca gcaacagata gaggacctcg ggtgagcccg gcagcagctg caggcttgcg
     1201 gacacggaga ccgatacgct cttttcgctg gcggcgtgcg cgagatcatc cacgacgcga
     1261 tccacgaccg tacttgccac cgtcgtttgc ccggcctcgg gggcggagcc cggctcgagc
     1321 cgcgcgagcg acaggagctg gctcatgaga tgagtggtac ggtcgatgcc cgcctgcagc
     1381 ttctcgaggg cacgccgcgc ttccgccggg gtcgccgccc gggcagccac ctgtgcctgc
     1441 gcacgcagcg ccgcgagcgg ggtgcgcagt tcgtgcgctg cgtcggcagt aaaacgccgc
     1501 tcgcgcgcca gcacctcggc gtgtcgggcc agcaggccgt tgagcgctac cccgagagca
     1561 cgagtctcga ccggcatctc ggccgttctc acgggcgaga gatcgtcggc gctacgaccg
     1621 gcgagatc
//
LOCUS       DATLKL010000045         3199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_102028, whole genome shotgun sequence.
ACCESSION   DATLKL010000045 DATLKL010000000
VERSION     DATLKL010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102028"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /gene="clpX"
                     /locus_tag="VM713_00610"
     CDS             <1..513
                     /gene="clpX"
                     /locus_tag="VM713_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315369.1"
                     /note="binds and unfolds substrates as part of the ClpXP
                     protease; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HVH82793.1"
                     /translation="SGIGFTSEVRDINTRPHGSDLFHDVEPEDLIKYGLIPELVGRLP
                     VVATLDELDEGALISILTEPKNALTKQYRRLFEMEGAELEFRDEALKAVAHRAMQRKT
                     GARGLRTILETVLLDTMYELPSMRNVSRIVIDESVIEGQTKPYIVYGTEEGGLASVDA
                     PRRASGSNHH"
     gene            721..3033
                     /gene="lon"
                     /locus_tag="VM713_00615"
                     /pseudo
     CDS             721..3033
                     /gene="lon"
                     /locus_tag="VM713_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
BASE COUNT          657 a         1022 c         1026 g          494 t
ORIGIN      
        1 agcggcatcg gcttcacctc cgaggtgcgc gacatcaata cgcgccccca tgggagcgac
       61 ctcttccacg acgtcgagcc cgaggacctc atcaagtacg gtctgatccc ggagttagtt
      121 ggccgccttc cggtcgtggc gacgctcgat gagctcgatg agggggcact catctcgatc
      181 ctcaccgagc ccaagaatgc cctcaccaag cagtaccggc ggctcttcga gatggagggc
      241 gcggagctcg agttccgcga tgaggcgctc aaggccgtgg cgcaccgcgc catgcagaga
      301 aagaccggcg cccggggact gcgcaccatc ctggagacgg tgctcctcga cacgatgtat
      361 gagctcccct cgatgcgcaa tgtgtcacgg atcgtaatcg acgagtcggt gatcgagggg
      421 cagaccaagc cctatatcgt ctacggcacg gaagaaggcg gcctcgcgag cgtcgatgcg
      481 ccgcgccgcg cctcgggctc gaaccaccac tgaagcgctg cgcgctggcg cagcaaagtt
      541 ccagccccgt gagctgcgtc acccttgaaa cgcgcagctt tagcctcatt tcccgctaaa
      601 ctactgtcat aaggaaccga tcgcggtgta gtgcaagcac tgcggtcggc gcggccatct
      661 gccatcgagg gcttgagcgt gtccgaaccc actactccta cgaccccgag ttccaccgtc
      721 atcgagaatg tgccgttgct accgttgcgg gacgtggtcg tctacccgca catggtgatc
      781 ccgctcttcg tcgggcgcga gaagtcgatc caggcgctcg atgtcgccat gcggaccgat
      841 aagcgcatca tgctggtcgc gcagaagcag gccgatatcg acgatccgaa ggcggacgac
      901 ctctaccgta tcgcgaccgt cgccacgatc cttcagttgc tcaagctccc cgacggcacg
      961 gtcaaggtgc tggtcgaagg cgtcgaccgc gcgcgcatcg agaagttaca cgccggggag
     1021 tactacgcct cggatgcgac gctactgccc gatgacgaag cgtacgacga gaaagagatg
     1081 gacgtgctct cgcgctcggt catctcgcag ttcgagcagt acgtgaagct caacaagaag
     1141 gtgccgcccg aggtgctcac cgcgctcgcc ggcatcgagc aggcggggcg cctggccgac
     1201 accgtcgccg ctcacatgtc cttgaagctc gccgagaagc agaaggtgct cgagatcctc
     1261 gacgtccgta agcgcctcga gcacatcctg gtcgccatcg agagcgagat ggacgtgctg
     1321 cagatcgaga agcgcatccg tgggcgcgtc aaggcgcaga tggaaaagag ccagcgcgag
     1381 tactacctca acgagcagat gaaggcgatc cagaaagagc tcggcgagat ggacgagggc
     1441 ggcaacgaga tctcagagct cgagcagaag atcgccaagg ccggcatgcc caaggaggcg
     1501 cgcgacaagg cggcgggcga gctcaacaag ctcaagatga tgtcgcccat gtcggccgaa
     1561 gccaccgtag tgcgcaacta catcgactgg ctgatcaagg tcccgtggaa gaagcgcacc
     1621 cgcatcatca aggacatcgc ccgcgcccag aaggtgctgg atgaggacca ctacgggctc
     1681 gaaaaggtga aggagcgcat cgtcgagtat ctcgccgtgc aacagcgcgt cgagaagctc
     1741 aagggcccga tcctgtgcct ggtgggcgcg ccgggagtcg gcaagacctc actcggtcag
     1801 agcatcgccc gctcgaccaa ccgcaagttc gtgcgcatgt ccttgggcgg cgtgcgcgat
     1861 gaggccgaga tccgcggcca ccgccgcacc tacatcggct cgatgccggg caagatcgtg
     1921 cagaacatgg ccaaggcggg tgtgcacaat ccgctcttcc tcctcgatga agtcgacaag
     1981 atgtcgatgg acttccgcgg cgatccctcg tcggctctcc tcgaggtgct cgaccccgag
     2041 cagaactcga ccttcaacga tcattacctc gaggtcgatc tcgatctctc ggaagtcatg
     2101 ttcgtgtgca ccgcgaacac cttgaacatt cccgccccac tgctcgatcg aatggaagtc
     2161 atccgtctcg cgggctacac cgaagacgag aagatgaaca tcgcgcggcg ctacctcgtg
     2221 ccgaagcaga tgaagagcaa cggcttgaag cccgacgagc tcaaggtctc cgacggcgcg
     2281 ctgctcgaca tcgtgcgctt ctatacgcgc gaggcgggcg ttcgcaacct cgagcgcgag
     2341 atcgctaaga tcggccgcaa ggccgtgaag cagatgctgc tgcacaagcg ggaccagaag
     2401 tcggtcgcgg tcacgccgcg caacctcgac aagttcctgg gcgtgcgccg cttccgctac
     2461 ggcaaggccg aggacatgaa ccgtgtcggc caggtcacgg gtctcgcctg gaccgaggtc
     2521 gggggcgagc tcctcacgat cgaggcagcg gtagtccccg gtaagggcaa gctccagcac
     2581 accggccagc ttggcgaggt gatgcaggag tcgatccagg ccgccatgac cgtggtgcgc
     2641 agccgcgcgc aggtgctggg gctcgagccc gagttctacc agaagcacga cgtgcacctg
     2701 cacatccccg agggcgcaac gccgaaggac ggcccgagtg ccggcatcgg catgtgcacg
     2761 gcgctcgtct cggcgctcac caagatcccg gtgcgctcgg atgtcgcgat gaccggggag
     2821 atcacgctgc gcggggaagt gctgccgatc ggggggctca aggagaagct cctggcggcg
     2881 caccgtggcg gcatcaagac cgtgatcatc ccgcatgaga acgagaagga tctggtggag
     2941 attcccgaca acatcaaggg caatatcgac gtcaagccgg tgaagtggat cgacgaggtg
     3001 ctcgcggtgg cgctcaccaa gcagccgctg ccgcagcatg ctacggatgc tccggcgacc
     3061 cccgaagcag ccgacgagga caagcgcggg catcgcaacg gccgccgtac caagcaggta
     3121 gtcccgccgg cgcattgact ctcgggcgca gccggcagaa ccacgctttt tgccggcggc
     3181 ccacggggta gtgtctgac
//
LOCUS       DATLKL010000046         1608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_34359, whole genome shotgun sequence.
ACCESSION   DATLKL010000046 DATLKL010000000
VERSION     DATLKL010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34359"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VM713_00620"
     CDS             <1..452
                     /locus_tag="VM713_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVH82794.1"
                     /translation="ALILVPASGGARMQEGILSLMQMPRTVIAVEQVREARLPYIVIL
                     TDPTTGGVSASFAMLGDVQIAEPGAVIGFAGARVIEETIKEKLPEGFQRAEYLLEHGM
                     VDMVAHRRELRETIIRVISLLTNREPPAPAIPLPKPAGNGQGAASVK"
     gene            424..>1608
                     /locus_tag="VM713_00625"
     CDS             424..>1608
                     /locus_tag="VM713_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HVH82795.1"
                     /translation="MAKAPPLSSDEILKRLLALHPKAIDLSLGRIERLLAALGHPEQH
                     LAPVVHVAGTNGKGSTIAFLRAFLEAAGYRVQAYTSPHLVRFAERIRLADGIIEETRL
                     AALLEECERVNAGAEITFFEITTAAAFLAFARATADILLLEVGLGGRLDATNVIERPA
                     LAVITPISIDHVDFLGDTIAKIAFEKAGILKPRVAAVVGPQPGEALGVIAGEADRASV
                     QLFRHGEQWTVAPTAGGMRYVGGAWRLDLPRPGLVGVHQLVNAGLAIAAAERLGGFRL
                     APDHLRLGLARVEWPARLQRLTCGPLVAALPGEGWELWLDGGHNPAAGETLAMQAERW
                     RDRPLGLVFGMLKTKDAVHFLAALASHAAATRTIAIPSEANSLSAEEACRLASSAGHR
                     AGA"
BASE COUNT          225 a          579 c          547 g          257 t
ORIGIN      
        1 cggccctcat cctcgtcccg gcctcgggcg gggcgcgcat gcaggaggga atcctctcgc
       61 tcatgcagat gccgcgcacg gtgatcgcgg tcgagcaggt gcgcgaggcg cggctcccct
      121 acatcgtcat tttgaccgat ccgaccacgg gcggcgtctc ggcctccttc gccatgctgg
      181 gcgacgtgca gatcgccgag ccgggggccg tgatcggctt cgccggcgcg cgggtgatcg
      241 aggagacgat caaggagaag ctgccagagg gcttccagcg cgccgaatac ctgctcgagc
      301 acgggatggt cgacatggtg gcccatcggc gtgagctccg cgaaaccatc atccgcgtga
      361 ttagcttgct tacgaaccgt gagccgccgg cgccggccat tccgctgccc aagcccgccg
      421 gcaatggcca aggcgccgcc tctgtcaagt gacgagatac tgaagcggct gctcgccctt
      481 catcccaagg ccatcgatct ctccttgggg cgaatcgagc gtctgctcgc cgccctcggg
      541 catcccgagc agcacttggc gcccgtggtg cacgtcgccg gcaccaacgg caagggttca
      601 accatcgcct tcctgcgcgc cttcctcgag gcggccggct accgggtcca ggcctacacc
      661 tcgccgcatc tcgtgcgttt cgccgagcgc atccggctgg ccgacggcat catcgaggag
      721 acgcgtctcg ctgcgctgct cgaggagtgc gagcgcgtga acgcgggcgc cgagatcacc
      781 ttcttcgaga tcaccaccgc cgccgccttc ctcgcgttcg cgcgcgcaac cgccgacatc
      841 ctcctcctcg aggtcgggct aggcggccgg ctcgacgcca ccaacgtgat cgagcggccg
      901 gcgctcgcgg tgatcacgcc gatctcgatc gatcatgtcg acttcctcgg cgacaccatc
      961 gcgaagatcg ccttcgagaa ggcgggaatc ctcaagccgc gcgtcgccgc ggtggttggc
     1021 ccgcagccgg gcgaagcgct cggcgtcatc gcgggcgagg ccgatcgcgc gagcgtgcag
     1081 ctgttccgcc atggcgagca gtggacggtc gccccgacgg cgggcggcat gcgctacgtg
     1141 gggggcgctt ggcggctcga cctgccgcgg ccgggtctgg tcggcgtgca tcagctcgtc
     1201 aatgccgggc tcgcgatcgc cgcggccgag cgcctcggag gctttcgttt ggcgcccgac
     1261 caccttcgcc tcggcttggc acgggtcgaa tggcctgcgc ggctgcagcg cctgacctgc
     1321 ggccctcttg ttgccgcgct gcccggggag ggctgggaac tctggctcga tggcggtcac
     1381 aatccggcgg ccggggagac gctcgcgatg caggccgagc ggtggcgcga ccggccactc
     1441 ggcctcgtct tcggcatgct caagaccaag gacgctgtcc atttcctggc cgcactcgcg
     1501 tcccatgcgg ccgcgacgcg cacgattgcc attccaagcg aggccaacag cctctcggcc
     1561 gaggaagcct gccgactggc gtcctcggcc ggccatcgcg ccggggca
//
LOCUS       DATLKL010000047         3211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_79384, whole genome shotgun sequence.
ACCESSION   DATLKL010000047 DATLKL010000000
VERSION     DATLKL010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79384"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1068
                     /gene="tsaD"
                     /locus_tag="VM713_00630"
     CDS             49..1068
                     /gene="tsaD"
                     /locus_tag="VM713_00630"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HVH82796.1"
                     /translation="MRVLAIESSCDESAAAVLDAEAGLLSHELYTQVALHRDYGGVVP
                     ELASRDHVRRLLPLAKAALARAATSPGDLSGVAYTAGPGLIGALLTGAALAASLAYAW
                     KIPAIGVHHLEGHLLAPLLESDPPPFPHVALLVSGGHTQLIEVAAIGSYRLLGETRDD
                     AAGEAFDKTAKLLGLAYPGGPQLAAIARRGTPGAYQFPRPMLDRAGLEFSFSGLKTAV
                     LHAVRAAPLTEQRRADIARAVEEAIAGTLTVKALRALDYTGLTALVVSGGVGANRTLR
                     EQLDAAARSHGARVYYPRIEFCTDNAAMIAVAGLARLKAGQHGGLAIEARAQWPLASL
                     PQLAA"
     gene            1094..1474
                     /gene="folB"
                     /locus_tag="VM713_00635"
     CDS             1094..1474
                     /gene="folB"
                     /locus_tag="VM713_00635"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HVH82797.1"
                     /translation="MTQRSGDRIFLHGLTTDCIIGFIDWERRVRQTVVVDLEMPVDCR
                     SASLTDEVADTLDYKKVAKRALEFINASEFKLVETLAHRLALLILEEFGVEWVRISLN
                     KPGAIRNSRDVGVVIERTRADLPR"
     gene            1485..1988
                     /gene="folK"
                     /locus_tag="VM713_00640"
     CDS             1485..1988
                     /gene="folK"
                     /locus_tag="VM713_00640"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HVH82798.1"
                     /translation="MPEVYVAAGSNVEPERRMQQAVAELTREFPGVRFSSWYRNRAVG
                     FDGDDFINLVAAFSTALPVHAVLERLHGIEGRCGRGRDAPRWAPRSMDLDVLLYGDLV
                     CEEPGLKLPRPDLLKRAYMLGPLAELAPEVLHPTAGLSAAELWRRFDRQAHPLERLVR
                     PAGGVGA"
     gene            complement(1995..2783)
                     /locus_tag="VM713_00645"
     CDS             complement(1995..2783)
                     /locus_tag="VM713_00645"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HVH82799.1"
                     /translation="MARANPDPAEALPAPREVLAGKTALVTGAARRVGAAIVRVLHGA
                     GANVLIHYRSSAEDAQRLAAELNATRERSVSLAECDLLDVTQHAGLIAAARQAFGGLD
                     ILVNNASTFYPTPLGDITEIDWDDLVGTNLKAPLFLAQAAAAALHERGGLIINLADIH
                     GMRPLRRYPVYSVAKAGVIMLTLSLARELGPHVRVNAVAPGPVMWAEDGMDAALQAKI
                     LARTALKRPGSAVDVARACLFFAADAPYVTGQILAVDGGRSIGW"
     gene            2819..>3211
                     /locus_tag="VM713_00650"
     CDS             2819..>3211
                     /locus_tag="VM713_00650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HVH82800.1"
                     /translation="MLPALGPEERRHSEAVAALVHAELAAAGGWLSFERFMELVLYAP
                     GLGYYSAGSWKLGSGGDFVTAPEVSELFGRCVAAQCAEVLRGTGGEILELGAGSGSLA
                     AAILTALEARGALPERYALLEVSADLAAR"
BASE COUNT          430 a         1178 c         1124 g          479 t
ORIGIN      
        1 cgccacctgc ggcccgcgcc gccgcagggg atggtctaga atttcctcat gcgcgtgctc
       61 gccatcgaat cctcctgcga cgagagcgcg gccgcggtgc tcgacgctga ggcgggtctc
      121 ctgtcgcatg agctctacac acaggtagcg ctgcatcgcg actatggcgg cgtagtgccc
      181 gagctcgcct cgcgcgatca cgtgcgccgg ctcctgccgc tcgcgaaggc ggcgctggcc
      241 cgcgcggcga cctcacccgg agatctctcc ggcgtcgcct acacggccgg cccgggactc
      301 atcggcgcac tgctcaccgg cgcagctctc gccgcgagtc tcgcctacgc ctggaaaatc
      361 ccggcgatcg gcgtgcacca tctcgagggg cacctgctgg cgccgctcct cgagagcgac
      421 ccgccgccgt ttccgcacgt ggcgctgctg gtctccgggg gacatacgca gctcatcgag
      481 gtggccgcga tcggcagcta ccggctcctc ggcgagacgc gcgacgacgc cgccggcgaa
      541 gccttcgaca agaccgcgaa gctcctcggg ctcgcgtatc ccggcggccc gcagctcgcc
      601 gcgatcgccc gccgcggcac gcccggggct taccaattcc cgcgtcccat gctcgatcgc
      661 gccgggctgg agttcagctt ctcgggcctc aagaccgcgg tgttgcacgc cgtgcgcgcc
      721 gccccgctca ccgagcagcg ccgcgccgac atcgcgcgcg ccgtcgagga ggcgatcgcg
      781 gggaccctca ccgtgaaggc gttgcgcgcg ctcgactaca ccgggctcac ggcgctcgtg
      841 gtctcgggcg gcgtcggcgc caaccgcacg ctgcgcgagc agctcgacgc cgccgcccgc
      901 tcgcatgggg cgcgcgtcta ctatccgcgg atcgaattct gcaccgacaa tgcggcgatg
      961 atcgccgtag ccgggctcgc gcgcctcaag gccgggcagc acggcggcct cgccatcgaa
     1021 gcgcgcgcgc agtggccgct cgcctcgctc ccgcagctgg cggcctgaca gccgagcctc
     1081 aaggcgtgcc cccatgacac agcgctccgg cgaccggatc ttcctgcacg gcctgaccac
     1141 cgactgcatc atcggcttca tcgactggga gcggcgtgtc cgtcagaccg tcgtggtgga
     1201 cctcgagatg ccggtcgact gccgcagcgc ctcgctcacc gatgaggtcg ccgacacgct
     1261 cgattacaag aaggtcgcca aacgggcgct cgagttcatc aacgcatcgg aattcaagct
     1321 ggtggagacg ctcgcgcacc gcctggcgct cctcatcctc gaggagttcg gggtcgagtg
     1381 ggtgcgcatc tcgctcaaca agcccggcgc catccgcaac tcgcgcgacg tcggcgtggt
     1441 gatcgagcgc acgcgcgctg acctgccgcg ctgagggctt atccgtgccg gaggtttacg
     1501 tcgcggcggg cagcaacgtc gagcccgaac gccgcatgca acaggcggtc gcggagctca
     1561 cgcgcgagtt tcctggcgtg cgcttctcct cctggtatcg caatcgcgcg gtcggcttcg
     1621 acggcgacga cttcatcaac ctggtcgccg ccttcagcac cgcgctgcca gtgcacgcgg
     1681 tgctcgagcg gctgcacggg atcgagggcc gctgcgggcg ggggcgcgat gcgccgcgtt
     1741 gggcgccgcg ctccatggat ctcgacgtgc tgctctatgg cgacctcgtg tgcgaggagc
     1801 cgggcttgaa gctcccgcga cccgacctgc tgaagcgggc ctatatgctc ggaccgctgg
     1861 ccgagctcgc ccccgaggtg ctgcacccga ccgccgggct cagcgccgct gagctgtggc
     1921 ggcgcttcga caggcaagcg caccctctcg agcggctcgt gcggcccgcg ggcggcgtcg
     1981 gggcctgagc catgtcacca gccgatgctg cgtccgccgt cgaccgcgag gatctggccc
     2041 gtgacgtaag gcgcgtcggc cgcgaagaac aggcacgccc gcgccacatc gaccgccgag
     2101 ccggggcgct tgagcgcagt gcgcgcgagg atcttcgcct gcagcgccgc atccatgccg
     2161 tcctccgccc acatgaccgg acctggggcg acggcgttca cccgcacgtg cgggccgagc
     2221 tcgcgcgcga gcgacagggt cagcatgatg acgccggcct tggccacgct gtacacggga
     2281 tagcggcgca gcggccgcat gccgtggata tcggcgagat taataatgag tccgccccgc
     2341 tcgtggagcg ccgcggccgc ggcctgtgcg aggaacagcg gcgccttgag gttggtgccg
     2401 acgagatcgt cccagtcgat ctcggtgatg tcgccgagcg gcgtggggta gaaggtcgag
     2461 gcgttgttca ccaggatgtc gagccctccg aaggcctgcc gcgccgcggc gatgagtccc
     2521 gcgtgctgcg tcacatccag caggtcgcac tcagcgaggc tcaccgagcg ctcgcgcgtc
     2581 gcgttgagct cggcggcgag gcgttgcgca tcctcggcgg agctgcggta gtggatcagg
     2641 acgttggcgc cggccccgtg cagcacgcgc acgatcgccg caccgacgcg gcgcgccgca
     2701 ccggtcacga gcgctgtctt gccggcgagc acctcgcgcg gtgccggcag cgcctcggcg
     2761 gggtcgggat tcgctcgcgc catgggggcc gattatgacc ccatcgcccg cgcgctcaat
     2821 gcttccggcc ctcgggcccg aggagcggcg tcacagcgag gccgtcgcgg ccctcgtgca
     2881 cgccgagctt gcggccgccg gcggctggct ctccttcgag cgcttcatgg agctcgtgct
     2941 ctatgcgccc ggactcggct actacagcgc cggcagctgg aagctcggca gcggcggaga
     3001 tttcgtcacc gctcccgagg tctcggagct gttcggccgc tgcgtggcgg cgcagtgcgc
     3061 cgaggtgctg cgcggcaccg gtggcgagat cctcgagctc ggcgcgggca gcggctcgct
     3121 ggctgccgcg attctcaccg cgctcgaggc ccgcggggcg ctgccggagc gttacgcgct
     3181 cctcgaggtg agcgctgacc tcgccgcccg c
//
LOCUS       DATLKL010000048         2062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_79405, whole genome shotgun sequence.
ACCESSION   DATLKL010000048 DATLKL010000000
VERSION     DATLKL010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79405"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..536)
                     /locus_tag="VM713_00655"
     CDS             complement(165..536)
                     /locus_tag="VM713_00655"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HVH82801.1"
                     /translation="MRSAATLVAATAALLLTAAAPEQPAMTAGDLAQLCNGADHVSVN
                     ACRIYILGVTQGIAVGIRIAAAKPPAARPCVPTGISAEELDASLKARLAALDSDSQQR
                     DAAGFIGAALAAQFPCPGGRR"
     gene            646..1893
                     /locus_tag="VM713_00660"
     CDS             646..1893
                     /locus_tag="VM713_00660"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HVH82802.1"
                     /translation="MIRVRYAAVCVLSLAAAVFAATVRAEDYTKPKVRAITAFVSLER
                     GSYERQIAETLAVLREAQRDFEQRGYTVESVRITTQPLAELLRGQSDAAALGFLKALD
                     DLATRENFLPNIGPAMVRDGDDPHTMQLLAKALSSLSQLNASAIIAGDDGIHWRVIRE
                     AARLVRYVADHSPHSQGTFNFAATAMLGPYGPFYPGAYHLGGGERLAIGFEGANVVQE
                     VFARTHGDFDAAVAELTRQLTAHARVAEEVGTLAASRHGWTFMGVDPTPAPLADVSIG
                     AAIEPFTGAKFGASGTMTAALVITTAVKAVAVKQIGYSGLMVPVMEDKLLARRWAEGT
                     YDTDALLAYSAVCGTGIDTVPLPGDVSEAQLMRIYGDVASLAWKWHKPLAARLQPVAG
                     RKPGEETDFRDPYLFNTRIHPLP"
BASE COUNT          318 a          705 c          700 g          339 t
ORIGIN      
        1 ggccgtctcg ggcgcgagca gctcgtagcg cgtgtactca tcggcctcgg tctgcgcgac
       61 cggctgcggg aaatagacgc cgtccctgcc agagggcgga ctctgcgcga gactcgcgga
      121 cgcagtcacc agcgccaggc gcagagcatg ggtgcagagc ctcttcatcg cctgcctccg
      181 gggcacggga attgcgccgc gagcgctgcg ccgatgaacc cggccgcatc gcgttgctgc
      241 gagtccgaat cgagcgcggc gagccgggct ttgaggcttg catcgagctc ttccgcggag
      301 atccccgtcg gcacgcaggg gcgggcggcc gggggctttg cggcggcgat gcggatcccc
      361 accgcgattc cctgggtgac cccgaggatg taaatgcggc aggcgttcac gctcacgtga
      421 tccgcgccgt tgcaaagctg cgcgaggtcg cctgcggtca tcgccggctg ttcaggcgcg
      481 gcggccgtga ggagcagggc ggccgtggcg gccacgaggg ttgcagctga tctcacgaga
      541 cgctcccggg tagtctttta cggcgtagcg tatcaaaggc caaacagagc ctgttcatgt
      601 tctaatgcgc caccaacaat ctcggccgcc atcgtgggag ggatcatgat ccgcgttcgc
      661 tacgccgcgg tttgcgtgct gtcgttggcg gcggcggtct tcgcagccac cgtacgggct
      721 gaggattaca ccaagcccaa agtccgcgcc atcaccgcct tcgtgagcct cgagcgcggc
      781 tcttacgagc ggcagatcgc cgagacgctc gcggtactgc gcgaggcgca gcgcgacttc
      841 gagcagcggg gttacacggt cgagtcggtc cgcatcacga cccagccgct cgccgagctg
      901 ctgcgcggcc agtcggacgc ggcggcgctc ggctttctca aggcgctcga cgacctcgcc
      961 acgcgggaga atttcctgcc gaacatcggg cccgcgatgg tgcgcgacgg cgacgatccg
     1021 cacaccatgc agctgctcgc caaggccctg agctcgctct cccagctcaa cgcgagcgcg
     1081 atcatcgccg gcgacgacgg cattcactgg agggtgatcc gcgaagccgc ccgcctagtg
     1141 cgctacgtgg ccgaccacag cccgcacagt cagggaacat tcaacttcgc ggcgactgcg
     1201 atgcttggtc cctacggacc tttctatccc ggtgcctacc atctcggcgg cggcgaacgc
     1261 ctcgcgatcg gcttcgaggg cgcgaacgtc gtccaggagg tattcgcgcg cacgcacggc
     1321 gacttcgacg ccgcggtcgc cgagctcacg cggcagctca ccgctcatgc ccgggtcgcc
     1381 gaggaggtcg gcacgctggc ggcgagccga cacggctgga ccttcatggg cgtcgatccc
     1441 acgccggcgc cgcttgctga cgtgtcgatc ggtgcggcga tcgagccctt caccggagcg
     1501 aagttcggcg cgagcggcac catgaccgcc gcgctcgtca tcacgaccgc cgtcaaggcc
     1561 gtcgcggtca agcagatcgg ctactcgggc ctcatggtcc cggtgatgga ggataagctc
     1621 ctcgcccgcc gctgggcgga ggggacctac gacaccgacg cgctgctcgc atactccgcc
     1681 gtgtgcggca ccgggatcga caccgtgcct ctgccgggtg atgtcagcga ggcgcagctg
     1741 atgcgtatct atggcgacgt ggcctcgctc gcctggaaat ggcacaagcc gctcgccgcg
     1801 cggctgcagc cggtcgcggg caggaagccg ggcgaggaga ccgactttcg cgatccctac
     1861 ctctttaata cgaggattca cccgctgcct taagttgcgc cgcgcgaatc tgtgaccggg
     1921 taccgtcgcg ggccggtcat ccgccacggc cgcggcgcgt tccattggct aacctcgtgc
     1981 gcgtaacctg actcgaggag cgtcggatga atcgcgtcat tccctgccgg ccgtggggcg
     2041 ttctggctat tctgaccgca tt
//
LOCUS       DATLKL010000049         3097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_11740, whole genome shotgun sequence.
ACCESSION   DATLKL010000049 DATLKL010000000
VERSION     DATLKL010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11740"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VM713_00665"
     CDS             complement(<1..456)
                     /locus_tag="VM713_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HVH82803.1"
                     /translation="MWPRIWGFLDRRLFGPASERTGIVGGALRLLRYPYAVLRDLWRG
                     EINLRAMSLVYTTLLSLIPLLAFSFAILRFFGGHRDLVPIVYEFFRPVGEPAATDLTT
                     RVVQFANRASNGVVGSVGFALLAWTLIGTIKKVEDSFNFLWHLEQPRSFA"
     gene            471..1313
                     /locus_tag="VM713_00670"
     CDS             471..1313
                     /locus_tag="VM713_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVH82804.1"
                     /translation="MPPVRAGYPERIVCLTEETTETLYLLGEERRIVGISGFTVRPPR
                     ARREKPKVSAFTSARTARIVELAPDLVLGFSDLQADIAAELVRAGLTVHIFNQRSVAG
                     ILAMIRTLGGMIGCEAKTAALLAELEGGLATVRAAAAALSPRPRVYFEEWDGPLISGI
                     GWVSELIQLAGGEECFPELAREPLGRNRIVADPLEVVRRAPDIVLGSWCGKKFRPEEL
                     AARPGWSAIPAVRTGFVREIKSAIILQPGPAALTDGVRALQVVITEWANASRAARGSA
                     HTPR"
     gene            complement(1267..2112)
                     /gene="djlA"
                     /locus_tag="VM713_00675"
     CDS             complement(1267..2112)
                     /gene="djlA"
                     /locus_tag="VM713_00675"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HVH82805.1"
                     /translation="MRLVLYVDMTAGMKWIGKLTGGLIGLAFGPIGVAVGVLLGHQFD
                     QAQEHDGEAGAPGDVAAIRERFFRATFRVMGYVAKADGRVSEREIAAARAVMSDLRLG
                     PAQVREAIALFGAGKQPDFDLVQELAGLRSACRGRGDILRVFLEIQVRAALAGNNLEG
                     PARPIMSRIAAATRISSFELAHIEAVLRIQQGGFYQQRPGDGAPGPGAGIAALEQAYR
                     VLETTAGASDAEVVKAYRRQLNRHHPDKLKANGLPESMIGHAKQRTQQIIEAYELIRA
                     RRGMH"
     gene            2111..3001
                     /locus_tag="VM713_00680"
     CDS             2111..3001
                     /locus_tag="VM713_00680"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HVH82806.1"
                     /translation="MPGPIVYRTRLTGLERLHEGKVRDIYAVDAATLLIVTTDRLSAF
                     DVVLPDPIPDKGGVLNSISNFWFARTAHLIPNHLTGRTLEGVVKDPAERALLEGRAVI
                     VRRLKALPIEAVVRGYLIGSGWKDYQRTGALCGMALPAGLPLAGELPQPLFTPATKAE
                     AGAHDENISFETVAQRIGAPLAAKVRDTALALYRFAAAHASARGIIIADTKFEFGVDA
                     AGTLTLIDEVLTPDSSRFWPADTYRPGVSPPSFDKQFVRDYLETLDWDKRAPGPKLPP
                     DVIARTSAKYREALARLTAP"
BASE COUNT          476 a         1101 c         1060 g          460 t
ORIGIN      
        1 cgcgaagctg cgcggctgct cgaggtgcca caggaagttg aagctgtcct cgaccttctt
       61 gatggtgccg atgagcgtcc aggcgagcag cgcgaacccc accgagccga ccacgccgtt
      121 gcttgcgcgg ttggcgaact gcacgacgcg cgtggtgaga tcggtggccg ccggctcgcc
      181 gaccgggcgg aagaactcgt acacgatcgg taccaggtcg cggtgcccgc cgaagaaccg
      241 caggatcgca aagctgaagg cgagcagcgg aatgagcgac aggagcgtcg tgtacaccag
      301 gctcatggcc cgcaggttga tctccccgcg ccacaggtcg cgcaggaccg cgtaggggta
      361 gcgcagcagc cgcagcgcgc cgcccacgat cccggtgcgc tcggaggccg gcccgaacag
      421 gcgccggtcg aggaagcccc agatgcgcgg ccacatggct taaggataca gtgccgccgg
      481 tgcgagctgg ctatcccgag cggatcgtgt gcctcaccga ggagacgacc gagacgctct
      541 acctcctggg cgaggagcgg cgcatcgtcg gcatctccgg cttcaccgtc cgcccgccgc
      601 gcgcccggcg cgagaagccc aaggtttcgg ccttcaccag tgcgcgcacc gcgcgcatcg
      661 tcgagctcgc gccggacctg gtgctcggct tctccgacct gcaggccgac atcgctgcgg
      721 agctggtgcg agcgggtctc accgttcaca tcttcaatca gcgcagcgtc gccggcatcc
      781 tggcgatgat ccgcacgctc ggcggaatga tcggctgcga ggcgaagacc gcggcgctcc
      841 ttgccgagct cgaaggcgga ctcgccacgg tgcgcgccgc ggcggcggct ctcagccccc
      901 gcccgcgcgt ctacttcgag gagtgggatg ggccgctgat ctccggcatc ggctgggtgt
      961 cggagctcat ccagctcgcc ggcggcgagg aatgctttcc ggagctcgcg cgcgagccgc
     1021 tcgggcgcaa ccgcatcgtc gccgacccgc tcgaggtggt gcggcgcgcc ccggacatcg
     1081 tgctcggctc gtggtgcggc aagaagttcc gtcccgagga gctggcggcg cgtcccggct
     1141 ggagcgcgat tccggcggtg cgcacgggct tcgtgcgcga gatcaagtcg gcgatcatcc
     1201 tgcagcccgg gccggccgcc ctcaccgacg gggtgcgtgc gctgcaggtg gtcattaccg
     1261 aatgggctaa tgcatcccgc gccgcgcgcg gatcagctca tacgcctcga tgatctgctg
     1321 ggtgcgctgc ttggcgtgac cgatcatcga ttccggcagc ccattcgcct tgagcttgtc
     1381 aggatggtgg cggttgagct gcctgcggta ggccttgacg acctccgcat cgctcgcgcc
     1441 tgcggtcgtc tccagcaccc ggtaggcctg ttcgagcgct gcgatgccgg cgccgggccc
     1501 cggcgcgcca tcgccgggtc gctgctgata gaagccgccc tgctggatcc gcagcaccgc
     1561 ctcgatgtgc gcgagctcga agctcgagat gcgggttgcg gccgcgatgc gactcatgat
     1621 cggccgcgcc ggcccctcga ggttgttgcc ggcgagcgcg gcacgcacct ggatctcgag
     1681 gaagacgcgc aggatgtccc cgcgcccgcg gcacgccgag cgcagcccgg cgagctcttg
     1741 cacgagatcg aaatccggct gcttgcccgc gccgaacagc gcgatggcct cgcgtacctg
     1801 cgccggcccg agcctgaggt ccgacatcac ggcacgcgct gcggcgatct cacgctcgga
     1861 cacgcggccg tcggctttgg cgacataacc catcacgcgg aaggtggcgc ggaagaagcg
     1921 ctcacggatc gccgccacgt cgccaggcgc gccggcctcg ccgtcgtgct cctgcgcctg
     1981 gtcgaactgg tgaccgagca gcacgccgac cgcgacgcca atcgggccga aggcgaggcc
     2041 gatgagtccg ccggtcagct tgccgatcca tttcatgccc gccgtcatgt caacgtatag
     2101 taccaagcgc atgccagggc ccatcgtcta ccgcacccgg ctcaccggtc tcgagcgcct
     2161 gcacgaaggc aaggtgcgcg acatctacgc tgtcgatgcc gcgacgcttc tcatagtgac
     2221 caccgatcgc ctgtcggcgt tcgacgtggt gctgcccgat ccgatcccgg acaagggcgg
     2281 cgtgctgaac tcgatctcga atttctggtt cgcgaggacg gcgcacctga tcccgaacca
     2341 tctgaccggg cgcaccctcg agggggtggt gaaggatccc gccgaacgcg cgctgctcga
     2401 aggccgcgcc gtgatcgtgc ggcgactgaa ggctctgccg atcgaggcgg tggtgcgcgg
     2461 ctacctcatc ggctcgggct ggaaagacta tcagcggacc ggagccctgt gcggcatggc
     2521 gctgccggcg ggcctgccgc tcgccggtga gctgccgcag ccgctgttca cgcccgccac
     2581 caaggccgag gcgggcgcgc acgatgagaa catcagcttc gagaccgtgg cgcagcgcat
     2641 cggcgcgccg ctcgccgcca aagtgcgcga tacggctctc gcgctctatc gcttcgccgc
     2701 ggcgcacgcc agcgcgcgcg gcatcatcat cgcggacacc aaattcgagt tcggcgtcga
     2761 cgccgccggg actctcacgc tcatcgacga ggtgctgacg cccgactcct cgcgcttctg
     2821 gcccgccgac acctatcgcc ccggagtcag tcccccgagc ttcgacaaac agttcgtgcg
     2881 cgactatctc gagacgctcg actgggacaa gcgcgcgcca gggccgaagc tgccgcccga
     2941 tgtgatcgcg cgcaccagcg ccaagtatcg cgaggcgctg gcgcggctca ccgcgccatg
     3001 agcaacggcg agccgcacgg cgcggaaaca gcagcagccg gcgcgacccc gccgcgcatg
     3061 ttctcggccg cggagatcgg cgcgatcgcg cacctct
//
LOCUS       DATLKL010000050         1514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_546, whole genome shotgun sequence.
ACCESSION   DATLKL010000050 DATLKL010000000
VERSION     DATLKL010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="VM713_00685"
     CDS             complement(<1..1501)
                     /locus_tag="VM713_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVH82807.1"
                     /translation="MVSETPNLAARLQALAAPGTVVIAASTRQLTGGLFDYEDLGPVE
                     IRGLAAPVTAARVLRQSAAESRFEALRSAAAPLIGRDQELDLLLRHWAEAKAGAGRAV
                     LISGEPGIGKSRLTAALSAHIESEPHTRLRYFCSPHDQDSALYPFIGQLERAAGFTRD
                     DTTATKLDKLEALLGDGAEPGDLSLLAEMLSLSGGERFPPLDLSPQRKKERTLAALLR
                     QLQALARRQPILMIFEDLHWIDPTSRELLDLTVEKITDLPVLLVVTYRPEFQPPWVGG
                     SQVTVIALNRLGRSEGATLVHQLAGNLGALPPDVVDEIVERTDGVPLFVEELTKAVVE
                     AGADRGYAPISAVPPSSLAVPATLHASLLGRLDRLGPSAKNVAQVGAAIGRDFSHELL
                     AAAAPLAEPELQEALRRLVEAGLVFQRGVPPMAEYLFEHALVQDTAYSTLLRGPRQAL
                     HRRIAEALEQRFPDLVETRPEIVAHHYGEAALPDKAITYRHCAGKSSVAK"
BASE COUNT          234 a          526 c          514 g          240 t
ORIGIN      
        1 atttggccac cgacgatttg cccgcgcagt gccgatacgt gatcgccttg tcgggcaggg
       61 cggcttcgcc gtagtgatgc gcgacgatct ccgggcgggt ttcgacgagg tccggaaacc
      121 gctgttccaa cgcctcggcg atccggcgat gcagggcctg gcgcgggccg cgcagcaggg
      181 tgctgtaggc ggtgtcctgg accagcgcgt gctcgaacag atattcggcc atcggcggca
      241 cgccgcgctg aaagacgagg cccgcttcga cgaggcggcg cagcgcttct tgcaattcgg
      301 gctcggcgag tggtgctgcc gctgccagca gctcatggga aaagtcgcgc ccgatggcgg
      361 cgccgacctg cgccacattc ttggccgagg ggcccagccg gtcgaggcgc cccagcagcg
      421 aggcgtgcaa ggtggctggc actgcgagcg aggacggcgg cacggcggat ataggtgcat
      481 agccgcgatc agcgccggcc tcgaccaccg ccttggtcaa ttcctcgaca aagagcggca
      541 ccccgtcggt gcgctcgacg atctcgtcga caacatccgg cggcaatgcc ccgagattgc
      601 cggcgagctg gtgcacgagc gttgcccctt cgctgcgccc gagccggttc agtgcgatca
      661 ccgtcacctg cgaccctccg acccagggcg gttggaactc ggggcggtat gtcacgacca
      721 gcagcaccgg caagtccgtg atcttctcga ccgtaaggtc gagcagctcg cgcgaggtcg
      781 gatcgatcca atgcaaatcc tcgaagatca tcagtatcgg ctgccgccgc gccagtgcct
      841 gaagctgccg cagcaatgcc gccaatgtcc gctccttctt gcgctgcggg ctgaggtcga
      901 gcggcgggaa gcgctcgccg ccggaaaggg acagcatctc ggcgagcagc gagaggtcgc
      961 ccggttccgc cccgtcgccg agtagcgcct ccagcttgtc gagctttgtt gcggtcgtgt
     1021 cgtcgcgggt aaaccccgcg gcgcgttcca gctggccgat aaacgggtag agcgcgctgt
     1081 cctgatcgtg cggtgagcag aaatagcgca gtcgggtgtg cggctcgctc tcgatatgtg
     1141 ctgacagcgc cgcggtaagg cgcgatttgc cgatccccgg ctcccccgaa atgagcaccg
     1201 cccggcccgc gccggccttc gcctctgccc aatgccgcag cagcaggtcg agctcctggt
     1261 cgcgcccgat cagcggcgcc gccgcggagc gcaatgcctc gaagcggctc tcggcggcgc
     1321 tctggcgtaa tacccgcgcc gcggtgaccg gcgcagccag gcccctgatc tcgaccggcc
     1381 cgagatcctc gtaatcgaac agcccgccgg tcaactgtcg cgtgctcgcg gcgatcacca
     1441 ccgtccccgg cgcggccagg gcctgcaacc gcgccgcgag gttcggcgtc tcgctgacca
     1501 ccgcctgctc ctgc
//
LOCUS       DATLKL010000051         2587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_113330, whole genome shotgun sequence.
ACCESSION   DATLKL010000051 DATLKL010000000
VERSION     DATLKL010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113330"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /gene="azu"
                     /locus_tag="VM713_00690"
     CDS             complement(<1..353)
                     /gene="azu"
                     /locus_tag="VM713_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HVH82808.1"
                     /translation="MTSLHATLAAVALSALVLPLSAHADPCKVEISGNDLLQYDKQEL
                     AVPATCKEITVTLHHIGKQPREAMGHDWVLVRTPDLAAVDQAGLSAGLAADYIAPGDK
                     RVLAHTKVIGGGQST"
     gene            complement(379..1434)
                     /locus_tag="VM713_00695"
     CDS             complement(379..1434)
                     /locus_tag="VM713_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVH82809.1"
                     /translation="MTETAVNAAAEAINTEVVIVGAGPCGLFQVFELGLLGIGAHVVD
                     SLQHPGGQCAELYPDKPIYDIPALPVCGAQELVDRLLQQIKPFKAPLHLGHEVTEFAR
                     ADNGRFHLGTAGGARFDAGAVVLAAGLGSFQPRRIGVEGAEAFEGGAIQYRIRNAAEL
                     AGKRLVIFGGGDSALDWTLELAPKAASLDLVHRRAEFRAAPASVARMRELVAAGRMRL
                     FEALPHSLIAADGALRGVNIRSADGSVQPLAADQLLVFFGLHPKLGPIADWGLALEKK
                     ALRVDPEKFQTSLPGVFAVGDINTYPGKKKLILSGFHEAALAAFAIQHHLYPQKKQFL
                     QYTTTSPVMHQRLGVPD"
     gene            1499..1960
                     /locus_tag="VM713_00700"
     CDS             1499..1960
                     /locus_tag="VM713_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVH82810.1"
                     /translation="MIRLSVNARDLTVDVDPETPLLWVLRDTLGLTGTKYGCGAALCG
                     ACTVHLAGAPVRSCVTPVSAVGARKVTTIEGLSAGRSHPVQKAWIELDVPQCGYCQSG
                     QIMSAAALLAHNPQPTDAEIDVAMAGNVCRCGTYQRIRAAIHRAAVLAAGG"
     gene            1988..>2587
                     /locus_tag="VM713_00705"
     CDS             1988..>2587
                     /locus_tag="VM713_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH82811.1"
                     /translation="MTTNAELHARRQQAVPRGVSNSLAVYAERACNAELWDVEGRRYI
                     DFASGISVLNTGHVHPRVSAAIAAQLAKLTHTCFQVTPTESYVALAEALNALAPGAGP
                     KKTIFLTTGAEAVENAIKIARFHTRRSAIISFSGGFHGRTLACISLTGKVQPYKAGFG
                     PMLPEIYHLPFPMPYHGITVEHTLEALEQLFKADVDPARV"
BASE COUNT          404 a          908 c          871 g          404 t
ORIGIN      
        1 ctggtgctct gaccgccgcc gatcaccttg gtatgcgcaa gcacgcgctt gtcgccaggc
       61 gcgatgtagt cggcggcgag cccggccgac aggccggcct ggtccacggc ggccaggtcc
      121 ggggtccgca ccagcaccca gtcgtggccc atcgcctcgc gcggctgctt gccgatgtga
      181 tgcagcgtca ccgtgatctc cttgcaggtc gccggcaccg cgagctcctg cttgtcgtac
      241 tgcagcaggt cgttgccgga gatctcgacc ttgcagggat ccgcgtgggc gctgagcggc
      301 aggactagag cgctcaacgc gacggcggcg agcgtggcgt gcagcgatgt catggcagcc
      361 tccttgtggc ttgaggtgtc agtccggtac tcccagccgc tgatgcatca ccgggctcgt
      421 ggtggtgtat tgcaggaact gcttcttctg cggatagagg tggtgctgga tcgcgaacgc
      481 agccagcgcc gcctcgtgaa agccggacag gatgagtttc ttcttgcccg ggtaagtgtt
      541 gatgtcgccg accgcgaaca ctcccggcag gctcgtctgg aacttctcgg gatcgacccg
      601 cagcgccttc ttctcgagcg cgagccccca gtcggcgatc gggccgagct tgggatggag
      661 gccgaagaaa accagcagct ggtccgccgc gagcggctgc accgagccgt cggcagaccg
      721 gatgttgact ccgcgcagcg cgccgtccgc ggcgatcagc gagtgcggca gcgcttcgaa
      781 cagccgcatg cggccggccg cgaccagctc gcgcatgcgc gccaccgagg cgggggctgc
      841 gcgaaactcc gcacggcgat gcaccaggtc gaggctcgcc gccttggggg cgagctcgag
      901 cgtccagtcg agcgccgaat cgccgccgcc gaagataacc aggcgcttgc cggcgagctc
      961 ggcggcattc ctgatgcggt actggatggc gccaccctcg aaggcctcgg cgccctcgac
     1021 gccgatgcgc cgcggctgaa acgagccgag ccccgcggca agcactaccg cgcccgcgtc
     1081 gaagcgcgcg cccccggccg tgccgaggtg gaagcgtccg ttgtcggcgc gcgcgaattc
     1141 cgtcacctcg tgcccgaggt gcaacggcgc cttgaagggc ttgatctgct gcagcaggcg
     1201 gtccaccagc tcctgcgcgc cgcacaccgg cagcgccgga atgtcgtaga tcggcttgtc
     1261 gggatagagc tcggcgcact gtccgcccgg gtgttgcagc gagtcgacca cgtgcgcgcc
     1321 gattccgagc agccccagct cgaacacctg gaacagtccg cacggaccgg cgccgacgat
     1381 gacgacctcg gtgttgatcg cctccgcggc ggcgttgaca gcagtctcgg tcaagatggc
     1441 gacattctag agggtctttc ctgcgctcgc atgcgatcgg ggtatagtac ggggccccat
     1501 gatcaggctg agcgtcaacg cccgggacct aaccgtcgac gtcgatcccg agacgccgct
     1561 cctgtgggtg ctgcgcgata cgctcggcct caccggcacc aagtacggct gcggcgcggc
     1621 gctgtgtggc gcgtgcaccg tgcacctcgc gggcgcgccg gtgcgctcgt gcgtgacgcc
     1681 cgtgagcgca gtaggcgcgc gcaaggtcac gaccatcgag ggcctctcgg ccggtcgcag
     1741 tcacccggtg cagaaggcct ggatcgagct cgatgttccc cagtgcggct actgccagtc
     1801 cggccagatc atgtcggcgg cggcgctgct cgcccacaac ccgcagccaa ccgatgccga
     1861 gatcgacgtc gccatggccg gcaacgtgtg ccgctgcggc acctaccagc gcatccgcgc
     1921 ggcgatccac cgtgccgcgg tgctcgccgc gggcggctga ggctttacaa ccttaagagg
     1981 aactgccttg actaccaacg ccgaacttca cgcgcgccgc cagcaggccg tgccgcgcgg
     2041 tgtcagcaac tcgctcgcgg tgtacgccga gcgcgcttgc aacgcggagc tctgggacgt
     2101 cgaaggccgc cgctacatcg acttcgcgag cggcatctcc gtgctcaaca ccggtcatgt
     2161 gcacccgcgg gtgagtgcgg cgatcgccgc acagctcgcg aagctcacgc acacctgctt
     2221 tcaggtgacg cccaccgaga gctacgtggc gctcgccgag gcgctcaacg cgctcgctcc
     2281 gggcgcgggg ccgaagaaga ccatctttct cacgaccggc gccgaggcgg tcgagaacgc
     2341 catcaagatc gcgcgctttc acaccaggcg ctcggcgatc atcagcttct cgggcgggtt
     2401 ccacggccgc acgctcgcgt gcatcagcct gaccggcaag gtgcagccct acaaggccgg
     2461 cttcgggccg atgctgcccg agatctacca cctgcccttt ccgatgccct atcacggcat
     2521 caccgtcgag cacacactcg aggcgctcga gcagctgttc aaggcggacg tcgacccggc
     2581 gcgcgtc
//
LOCUS       DATLKL010000052         2354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_90825, whole genome shotgun sequence.
ACCESSION   DATLKL010000052 DATLKL010000000
VERSION     DATLKL010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90825"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1837
                     /locus_tag="VM713_00710"
     CDS             38..1837
                     /locus_tag="VM713_00710"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH82812.1"
                     /translation="MSEQVEIERASQALNQGKFAECAAICGAIVARDAHNAVAAHLLG
                     LAVKETGDFAQGEQWLRLSVQLEPQRADFHANLANLLRRREKFKQAERFYRRALQLAP
                     GHRAARCSLALTLNDLGRFEESEAVCRELLAADPGDAEAWIVIGMNLSRLERLTEAEA
                     AYRQALELDPGNRVAHHNLGTLLTRMGRPEAGLAALESAQALGEGGFELAFNRGRTLM
                     EMNEVEAAEREFERAVSLQPTNPEAHLQLARVRYARGDPQFARSLATATAENRANAGL
                     QMLFGEVLWRAGELRSAEVVLQELVARQLGGAHVQSILACVLLETGRLKDAERHALEA
                     ATAAPEDGVVNENLVSVLLSCGRPEEAMLFIRQQRRRRPGAQAWLAFEATAARLLDPE
                     LYHRLYDYERLVRTFELEPPAGWSSMEELNAALLEALGSRHRLQHHPLDQTLRHGTQT
                     PRSLLAEGDPAIKAILAAFHGPIAEYRRLLGSDAAHPMSAGNIGEMRYTGAWSVRLHR
                     LGYHVNHFHPEGWLSSAYYVQTPPETEDAVTRWGWIKFGEPRYPVPGLKPERFVQPRP
                     GRLVLFPSYMWHGTTPIYGDAPRLSIAFDVKPA"
     gene            complement(1857..>2354)
                     /locus_tag="VM713_00715"
     CDS             complement(1857..>2354)
                     /locus_tag="VM713_00715"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HVH82813.1"
                     /translation="IRHAPQSPLGQWKELNHSQRWSALFLHEDGEAHPDNIARCPRTM
                     AALAQAPQVNIPARGPTAFFSRLEPKTRIPPHTGSTNTRLTVHIPLVVPPGCGFRVGS
                     EVREWQPGTALIFDDTIEHEAWNDSDQPRVIFIFDIWNPLLTAAERALMTVATAGIAE
                     FFDEA"
BASE COUNT          388 a          778 c          814 g          374 t
ORIGIN      
        1 tctgtgtgtc gaaatgtcag cgcggcataa ttgacccatg tcggaacagg tcgagatcga
       61 gcgcgcatcg caggcgctca accagggaaa atttgcggag tgcgcggcga tctgcggcgc
      121 catcgtggcc cgcgatgcgc acaatgccgt ggctgcgcac ctcctcggcc tcgctgtcaa
      181 ggagaccggt gacttcgccc agggagagca gtggctgcga ctgagcgtgc agctcgagcc
      241 acagcgagcc gacttccacg ccaaccttgc taacctcctg aggcgccggg agaaattcaa
      301 gcaggcggaa cgtttttacc gccgggcact gcaactggcg ccgggacatc gcgccgcgcg
      361 gtgcagcctc gccctgacgc tgaatgatct cgggcgcttt gaggagtccg aggcggtctg
      421 ccgcgagctg ctggccgccg atccaggtga tgccgaggcc tggatcgtga tcggtatgaa
      481 cctgagccgt ctcgagaggc tcaccgaggc ggaggcggcc tatcgccagg cactcgagct
      541 ggatccgggc aatcgcgtcg cccatcacaa tctcgggacg cttctgaccc ggatgggccg
      601 acccgaggcc ggtctcgcgg ccctcgagag cgcgcaagcg ctcggcgagg gaggcttcga
      661 gctggcgttc aaccgcggcc gcaccctgat ggagatgaat gaggtggagg cggcggagcg
      721 cgaattcgag cgtgccgtgt cgctgcagcc gaccaatcct gaggcacatc tgcagctcgc
      781 ccgggtgcgc tacgcacgcg gcgatcctca gttcgcgcgc tcgctggcca cggccaccgc
      841 cgagaatcgg gccaacgccg gactgcagat gctgtttggc gaggttctct ggcgtgccgg
      901 agagctgcgc tcggccgagg tcgtgctgca agagctggtg gcgcgccaac tcgggggagc
      961 gcacgtgcag tcgatcctgg cgtgcgtcct tctcgagacc ggacgcctca aggacgccga
     1021 gcgccacgcg ctggaggcgg ccacagccgc ccctgaggat ggcgtcgtca acgagaacct
     1081 ggtgagcgtg ctgctctcct gcgggcgccc ggaggaggcg atgctcttca tcaggcagca
     1141 gcgccggcgg cgcccgggag cccaggcctg gctcgcgttc gaggcgacgg cggcgcggct
     1201 gctggacccg gagctctatc accgccttta cgattacgag cggctggtca ggaccttcga
     1261 gctcgagccc ccggcaggct ggtcctccat ggaggagctg aacgcggccc tgctcgaggc
     1321 gctcgggagc cggcaccgcc tgcagcacca cccgctcgac cagactctgc gccacggcac
     1381 ccaaactccg cgcagcctgc tcgccgaggg cgacccggca atcaaggcga tcctcgccgc
     1441 cttccacggg ccgatcgccg agtaccggcg cctgctcggc agcgacgccg cccatccaat
     1501 gtcggcaggc aatatcggtg agatgcgtta cacgggcgca tggtccgtgc ggctgcatcg
     1561 gctcggatat cacgtcaatc atttccatcc cgagggctgg ctgagctcgg cgtactacgt
     1621 gcagacgccg ccggagacgg aggacgccgt gacgcgctgg gggtggatca agttcggtga
     1681 gccgcgctac cccgtgccgg gactcaagcc ggagcgtttc gtccagccac gccccggtcg
     1741 actggtgctg tttccgtcct acatgtggca cggcaccaca ccaatctacg gcgatgcgcc
     1801 gcgcctcagc atcgcttttg atgtgaagcc ggcctgaggc tcctcctggc cgcgggtcag
     1861 gcttcgtcga agaactcggc gatgcccgcg gtcgccaccg tcatcagcgc gcgctccgca
     1921 gccgtcagca gcggattcca aatatcgaaa atgaagatga cgcgcggctg atcgctatcg
     1981 ttccacgcct cgtgctcgat ggtgtcatcg aaaatgagcg ccgtgcccgg ctgccactcg
     2041 cgcacctcgg agccgacgcg aaaaccgcag ccgggcggca cgacgagcgg gatgtgcacc
     2101 gtgagacgcg tgttggttga gccggtatgc ggcggaatgc gcgtcttcgg ctcgaggcgc
     2161 gagaagaacg cagtcggccc gcgagcggga atgttcacct gcggcgcctg tgccaacgcg
     2221 gccatggtgc gtggacagcg ggcgatgttg tcggggtgag cctcgccgtc ctcgtgcaga
     2281 aaaagtgcgc tccagcgctg cgagtggttc agctccttcc actggccgag gggggactgc
     2341 ggcgcatggc ggat
//
LOCUS       DATLKL010000053         1702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_147144, whole genome shotgun sequence.
ACCESSION   DATLKL010000053 DATLKL010000000
VERSION     DATLKL010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147144"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VM713_00720"
     CDS             complement(<1..753)
                     /locus_tag="VM713_00720"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HVH82814.1"
                     /translation="MKKPPRSNAFYAQSGGVSAVINASAAGVIETAMRSKHIGKVYAG
                     RDGIVGALTEDLIDVSRESAATIRALKHTPAGAFGSARFKLKSIEQNRAEYQRLIDVF
                     RAHDIRYFFYNGGNDSMDTALKVSQLGASLGYPITCVGVPKTVDNDLPHTDCCPGFGS
                     VAKYVAVSTREASLDVASMARTSTKVFVFEVMGRHAGWIAAAVGLAGRGMDEPPHVIL
                     FPEVPFDGRRFLDRVKQCVTDYGYCVIVVSEGA"
     gene            897..>1702
                     /locus_tag="VM713_00725"
     CDS             897..>1702
                     /locus_tag="VM713_00725"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HVH82815.1"
                     /translation="MRIVLLGAPGSGKGTQAQRLAERGGVPQISTGDLLRAAVAHGTE
                     LGLKAKAAMESGGLVDDSLVLGMIRERLAEPDARAGFILDGFPRNLVQAAALDGLLAE
                     LKQPLDAVVLLEVEAGELVRRISGRRCCADCGRVFNVLTSPPTGAAAVCPKTGGPHRL
                     VQRPDDNEATVIERLRVYEEKTRPLIAFYQSRGLLRVIDAQGDVDAVTRRLARALAAP
                     AAVSVRRSVARRPARTRPPTRAAKRRAKPTRKKTPARPAPKGGRRVAAAP"
BASE COUNT          256 a          565 c          629 g          252 t
ORIGIN      
        1 agcgccctcg gacaccacga tgacgcagta gccgtagtcg gtcacgcact gcttgacgcg
       61 gtcgaggaag cgccgcccgt cgaacggcac ctcggggaac aggatgacgt gcggaggctc
      121 gtccatgccg cgcccggcga gaccgacggc ggcggcgatc cagccggcgt gccggcccat
      181 cacctcgaag acgaacacct tggtcgaggt gcgcgccatc gaggccacgt cgagggaggc
      241 ctcgcgcgtc gagaccgcga catatttcgc gaccgagccg aagcccgggc agcagtcggt
      301 gtgcgggaga tcgttgtcga ccgtcttcgg cacgccgacg caggtgatcg gatacccgag
      361 ggaggcgccg agctgcgaca ccttgagggc ggtgtccatc gagtcgttgc cgccgttgta
      421 gaagaagtag cggatgtcgt gcgcccggaa cacgtcgatc agacgctggt actcggcgcg
      481 gttctgttcg atgctcttca acttgaaccg ggccgagccg aaggcgcccg ccggcgtgtg
      541 cttgagcgcg cggatggtgg ccgcgctctc gcgcgagacg tcgatcaggt cctcggtgag
      601 cgcgccgacg atgccgtcgc gcccggcgta caccttgccg atgtgcttcg agcgcatggc
      661 tgtctcgatg acgccggccg ccgaggcgtt gatgacggcg gacactccgc cggactgggc
      721 atagaaggca ttgctgcgcg gaggcttttt catggtgttg gagttgtcgg gcaggagatc
      781 ccgccaagaa tagcgcaggc ggagcaccgc ggggcagcgc gttgacctgg caccgggcgc
      841 tcggtaacgt ggcccactcc gagctgccag cgcagcgttc ttgcaccgat aaaaacatgc
      901 gcatcgttct gttgggggcg ccgggatccg gcaagggcac ccaggcacaa cgcctggccg
      961 agcgcggtgg cgttccgcag atctcgaccg gtgatctgct gcgcgctgcc gtggcgcacg
     1021 gcacggagct gggactcaag gccaaggcgg ccatggagag cggcgggctg gtcgacgatt
     1081 cgctggtgct cggcatgatc cgcgagcgcc ttgccgagcc cgacgcgcgg gccgggttca
     1141 tcctcgacgg ctttccgcgc aacctcgtgc aggcggcagc gctcgacggc ctgctggcgg
     1201 agctcaagca gccgctcgat gcggtggtgc tgctcgaggt cgaggcgggt gagctggtgc
     1261 ggcgcatttc cgggcggcgc tgctgcgccg attgcgggcg cgtcttcaac gtgctcacct
     1321 cgccgccgac gggcgccgcg gcggtgtgcc cgaagaccgg cggtccacac cgtctcgtac
     1381 agcgtccgga cgacaacgag gcgacggtga ttgagcggct gcgggtgtac gaggagaaga
     1441 ctcggccgct gatcgctttc taccagtcgc gtggcctgct gcgcgtcatc gacgcgcagg
     1501 gcgatgtcga tgcggtcacg cggcgactcg cgcgggcgct cgcggcgccg gccgcggtca
     1561 gcgtccgccg gagcgtggcg cgcaggcccg cacgcactcg gcccccgacc cgtgcggcca
     1621 agcgccgcgc taagccaacg cgcaagaaga cgccggcgcg ccccgccccc aagggtgggc
     1681 gccgcgtcgc tgccgcgccc ag
//
LOCUS       DATLKL010000054         3081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_45593, whole genome shotgun sequence.
ACCESSION   DATLKL010000054 DATLKL010000000
VERSION     DATLKL010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45593"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VM713_00730"
     CDS             <1..973
                     /locus_tag="VM713_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVH82816.1"
                     /translation="AFHSDCAQAAGKVPLDVQGLPVDFLSFTAHKLYGPKGAGALYVR
                     ASARGLLQPVTFGGGQEHGWRPGTLATHQIVGFGAACEIARGELPAERPRLTALRERL
                     WSRLSKLGGVHLNGAGAPRVPGILNVSFEGVEGESLVSALPELAVSTGAACNSASAEA
                     SYVLRALGRDALLAGSSLRLSLGRFTRDEEVDLAAAALAREVARLRALSPASGVAGAL
                     ELGAGEESGTVVSGEAGGPRQETWIRFHLLIAGDTVKDARFQAFGCPHTVDVAAWLCR
                     EVKGRARGSLPPGGPADWAASRSVPVEKLGRLLVIEDALRACLLQWP"
     gene            995..1318
                     /gene="iscA"
                     /locus_tag="VM713_00735"
     CDS             995..1318
                     /gene="iscA"
                     /locus_tag="VM713_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HVH82817.1"
                     /translation="MAISLTESAANRVKSFLAARGGGVGLRLGVRKTGCSGFAYVVNY
                     AESQQPGDTVFEDRGVRVFVDSASLGLLDGTIVDFVKEGLNEAFRFRNPNVKGECGCG
                     ESFSV"
     gene            1492..1920
                     /gene="ndk"
                     /locus_tag="VM713_00740"
     CDS             1492..1920
                     /gene="ndk"
                     /locus_tag="VM713_00740"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HVH82818.1"
                     /translation="MALERTLSIVKPDGVRGNLIGEVYRRFEKAGLSIIAARMLHLSQ
                     REAEGFYAVHRERPFFKDLVRYMSSGPVVVQVLEGENAIARNRDLMGATDPKKAAPGT
                     IRADLAASIEQNVVHGSDAPDTALREIAYFFSTTELHSRN"
     gene            1939..3069
                     /gene="rlmN"
                     /locus_tag="VM713_00745"
     CDS             1939..3069
                     /gene="rlmN"
                     /locus_tag="VM713_00745"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017347778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HVH82819.1"
                     /translation="MSTPAAERTNLLGLARPALETFVAQLGSRPFRARQLMNWIYKRG
                     TSEFAAMTDLAKDFRARLAAAAEVRPPEIVAVQRSADGTVKWLLRADAAQAFEMVFIP
                     EPDRGTLCISSQVGCALDCSFCSTAQQGFNRNLTTAEIVGQVWLASAELAREDAAAGA
                     AAAERRVTNVVLMGMGEPLANFRNVVPALDIFLDDFGCDLSRRRVTLSTSGLVPQINR
                     LAEVSNVALAVSLHAPDDALRNELVPINRKHPIAELLPACWHYLDEQNGRSVTFEYVM
                     LDAVNDSPAQARALARLLKGHPAKVNLIPFNPFPGTRYTRSPAPVIQRFRDELLQRGV
                     LATVRRTRGEDIDAACGQLAGRVIDRTTVRLGSKVIGVAVQP"
BASE COUNT          467 a         1071 c         1051 g          492 t
ORIGIN      
        1 cgccttccac agcgactgtg cgcaggccgc cggcaaggtg ccgctcgatg tccaagggct
       61 gccggtcgac ttcctctcgt tcaccgccca caagctctac ggcccgaagg gagccggcgc
      121 gctgtacgtg cgcgcgagcg cccgcgggct gctgcagccg gtcactttcg gcggcgggca
      181 ggagcacggt tggcgtcccg ggacgctcgc gacccaccag atcgtcggct tcggcgccgc
      241 ctgcgagatc gcccgcggcg agctgcccgc cgagaggccg cggcttaccg cgctgcgcga
      301 gcgcctgtgg agccgcctga gtaagctcgg cggtgtccac ctgaacgggg cgggcgcacc
      361 gcgcgtaccg ggcatcctca acgtgtcctt cgagggcgtc gagggcgaga gcctggtgag
      421 cgcgctcccg gagctcgcgg tatcgaccgg ggcggcgtgc aactccgcgt ccgcggaagc
      481 gtcctatgta ctgcgggcgc tcgggcgcga tgcgctgctt gccgggagct cgctgcgctt
      541 gagtctcggg cgcttcaccc gggacgagga ggtggatctg gccgccgccg ccctcgcccg
      601 tgaggttgca aggctgcggg cgctctcgcc ggcctcaggt gtggcgggcg ccctcgagct
      661 cggcgcgggc gaggagagtg gcacggtcgt gagcggcgag gccgggggac cccggcagga
      721 gacctggatc cgctttcacc tgctcatcgc gggcgacact gtgaaagacg cacgttttca
      781 ggcctttggc tgcccgcata ctgtggatgt ggccgcctgg ctgtgccggg aggtgaaggg
      841 ccgggcccgc gggtcgctcc ctccgggagg accggcggac tgggcggcga gccgctcggt
      901 tccggtggag aagctcgggc ggctcctggt gatcgaggac gcgctgcgtg cctgcctatt
      961 gcaatggccg taagcggtag aatatcgagg gtcgatggca atctcgctga cagaatccgc
     1021 ggctaatcgc gtcaagagct tcctcgctgc ccggggcggc ggagtcggcc tgcggctcgg
     1081 cgtgcgcaag accggctgct cgggcttcgc ctacgtcgtc aactacgccg aatcccagca
     1141 acccggcgat accgtgttcg aggaccgcgg ggtgcgggtg ttcgtcgact ccgcgagcct
     1201 cggtctcctc gatgggacga tcgtcgattt cgtcaaggaa gggctgaacg aggcgttccg
     1261 cttccgcaat ccgaacgtga agggcgaatg cggctgcggg gagagcttct cggtctgaac
     1321 gttccggggc gcccgcgcga ggacggcgcg gcggccccaa aaaagcctta ccaaaccggt
     1381 acttccgctg cgccgcaaat tgccgcggga gcgcgagcca agttaaactg gcgcgcttgc
     1441 ccgcgcacgc cgcgcgcatg tcatccgcac taaacacaag gaccccatcg aatggcgctc
     1501 gagcgcaccc tgtccatcgt caagcccgac ggcgtccgcg gcaatctgat cggcgaggtc
     1561 tatcgccgct tcgagaaggc gggactttcg atcattgccg cgcgcatgct gcacctgtcg
     1621 cagcgcgagg ccgagggttt ctatgccgtt caccgcgagc gtcccttctt caaggatctg
     1681 gtgcgctaca tgagctcagg cccggtggtc gtgcaggtgc tcgaaggcga gaatgccatc
     1741 gcgcgcaacc gcgatctgat gggcgcgacc gatccgaaga aggccgcccc cggtacgatc
     1801 cgcgccgatc tcgccgcgag catcgagcag aacgtcgtgc acggctcgga cgcgcccgac
     1861 acggcgctgc gcgagatcgc ctatttcttc agcactacgg agctgcactc gcgcaactga
     1921 agcagcgcga gtttccccat gagcacgcca gccgccgagc gcacgaatct gctgggactt
     1981 gcgcgccccg cgcttgagac ctttgtggcg cagctcggca gccggccttt ccgcgcgcgg
     2041 cagctcatga actggatcta caagcgcggc acgagcgagt tcgcggcgat gaccgatctc
     2101 gccaaggact ttcgcgcccg gctcgccgct gccgccgagg tgcgaccgcc ggagatcgtc
     2161 gccgtgcagc gctccgccga cggcaccgtc aagtggctgc tgcgtgcgga cgccgcccag
     2221 gcgtttgaga tggtgttcat tcccgagccc gaccgtggga cgctgtgcat ctcctcgcag
     2281 gtcggctgcg cgctcgactg ctcgttctgc tcgaccgcgc agcagggctt caaccgcaac
     2341 ctcaccacgg ctgagatcgt cggccaggtg tggctcgcga gcgccgagct cgcccgcgag
     2401 gacgccgcgg cgggggctgc cgcggccgag cgccgcgtga ccaacgtcgt gctcatgggc
     2461 atgggcgagc cgctcgcgaa cttccgcaac gtggtgccgg cgctcgatat cttcctcgat
     2521 gacttcggct gcgatctgtc gcgtcggcgt gtgacactct ccacctccgg gctcgtgccg
     2581 cagatcaacc ggctcgccga ggtgagcaac gtcgcgctcg cggtctcgct gcacgcaccc
     2641 gacgatgcgc tgcgcaacga gctcgtgccg atcaaccgca agcacccgat cgccgagctt
     2701 ctgcccgcct gctggcacta tctcgacgag caaaacggcc gcagcgtcac tttcgagtac
     2761 gtcatgctcg atgccgtgaa cgactccccg gcgcaggcgc gcgctctggc gcggctgctc
     2821 aaaggccacc ccgccaaggt gaatctcatc cccttcaacc cgttccccgg cacccgctac
     2881 actcgctcgc ctgcgcccgt catccagcgc ttccgcgacg agctgctgca gcgcggcgtg
     2941 ctcgccaccg tgcgccgcac gcgcggcgag gacatcgatg ccgcctgcgg ccagctcgcc
     3001 gggagagtaa tcgatcgcac gacggtgcgg ctcgggagca aagtcatcgg cgtcgccgtc
     3061 caaccatgag aaaaaaaaca t
//
LOCUS       DATLKL010000055         3060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_192329, whole genome shotgun sequence.
ACCESSION   DATLKL010000055 DATLKL010000000
VERSION     DATLKL010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192329"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /gene="recN"
                     /locus_tag="VM713_00750"
     CDS             <1..1611
                     /gene="recN"
                     /locus_tag="VM713_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HVH82820.1"
                     /translation="LQLLAGGRAGAEVVRHGAERAEVSATFDLTQAPRELKQWLEEQS
                     IGAGAELIMRRVVGNDGRSRAYLNGQSVPLQSLRDAGNILIDIHGQHEFQSLTRSAQQ
                     RELLDGYGALTGLTSQVGIAHRVWLGLLNRTLELESKARDRDAKLDLLRYQVQELEGL
                     HLMDGELASLSEESARLANRGRLATGAQVALGELYDNDAASAHAAVSRALAALRSLAT
                     LDTKLAQVLPLLEEGAVQIREAARELERYGDTLEVDATRQDEVERRLAAIEELSRKHR
                     IAPAQLPERTTELRAEFEGLERAELDLAQLRKELGGALEGYRTQAQDLSGKRHKAARA
                     LARDITTRMQTLGMTGGRFEADVTQDGNADPTEHGIDRIEFRVTANPGQPPRALAKVA
                     SGGELSRLSLAVQVSCAAQNMRCMVFDEVDSGIGGAVAEIVGRELRKLGERGQVLCVT
                     HLPQVACQAHHHLRVTKLTDGRTTRTTLNELIPGERVEELARMLGGVEVTDKAREHAR
                     DMLNAAERGGAVPVTASVSSRPPAARRK"
     gene            complement(1644..2078)
                     /gene="fur"
                     /locus_tag="VM713_00755"
     CDS             complement(1644..2078)
                     /gene="fur"
                     /locus_tag="VM713_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HVH82821.1"
                     /translation="MEGKDLRKAGLKVTLPRLKILEILEGSTTRHLSAEDIYRMLLES
                     NEDIGLATVYRVLTQFEAAGLVTRHHFEDGMAVFELNQGTHHDHIVCLDCGRVEEFMD
                     SGIESRQTAVAHKLGFTIRDHSLILYGHCERPNCPYRKHKTS"
     gene            2196..2549
                     /locus_tag="VM713_00760"
     CDS             2196..2549
                     /locus_tag="VM713_00760"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HVH82822.1"
                     /translation="MTALLLAALPLSGCIYRMNIQQGNYLEGKTVAQLQVGMTRSQVR
                     YLLGSPMVPDIFDKDRWDYLYYFKSGHLQRPEQRHLVVLFKEDKVASFQHDHIPDSAP
                     RAPDNDISIQKFPKI"
     gene            complement(2546..2857)
                     /locus_tag="VM713_00765"
     CDS             complement(2546..2857)
                     /locus_tag="VM713_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HVH82823.1"
                     /translation="MKRCVVAYATPARQYLWSVELPGEATIADALAAARRAAERESAG
                     LDPALWDLAPVGVFGELRTRAARVEDGDRIELYRPLRADPRARRRERVQRERHRSTKG
                     R"
     gene            complement(2854..>3060)
                     /locus_tag="VM713_00770"
     CDS             complement(2854..>3060)
                     /locus_tag="VM713_00770"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVH82824.1"
                     /translation="VEMRLVSGPFRALEGRWLLTPVEGGCRVELTMRFAFRNALTGLL
                     FEPRFAETVGSLVDAFVARARDST"
BASE COUNT          486 a         1079 c         1061 g          434 t
ORIGIN      
        1 ctgcagctgc tcgccggcgg gcgcgcgggc gccgaggtgg tgcgccatgg tgcggagcgc
       61 gccgaggtgt ccgccacctt cgatctcacg caggcgccgc gcgagctcaa gcaatggctc
      121 gaggagcagt cgatcggcgc cggtgccgag ctcatcatgc gccgcgtggt cgggaacgat
      181 ggccgctcgc gcgcttacct caacggccag tcggtcccgc tgcaatcgct gcgcgatgcc
      241 ggcaacatcc tcatcgatat ccatggccag cacgagttcc agtccctgac gcgcagcgct
      301 cagcagcgcg agctgctcga cggctatggc gcgctcacgg ggctcaccag ccaggtcggg
      361 atcgcgcacc gcgtctggct cggactcctc aaccgcacgc tcgagctcga gagcaaggcg
      421 cgcgaccgcg acgccaagct cgacctgctg cgctaccagg tgcaggagct cgagggcctg
      481 cacctcatgg acggcgagct ggcgagcttg agcgaggaga gtgcgcggct cgccaaccgc
      541 ggccgcctgg cgacgggcgc ccaggtggcg ctcggtgagc tctacgacaa cgacgccgcg
      601 agcgcgcatg cggctgtgag ccgggcgctc gcggcgctgc gctccctggc gacgctcgat
      661 accaagctcg ctcaggtgct gccgctcctg gaggaaggcg cggtgcagat ccgcgaggcg
      721 gcccgcgagc tcgagcgcta cggcgacaca ctcgaggtcg acgccacgcg ccaggacgag
      781 gtcgagcggc gcctggcggc gatcgaggag ctctcgcgca agcaccgcat cgcgccggcg
      841 cagctcccgg agcgcaccac agagctgcgc gcggagttcg aggggctcga gcgcgccgag
      901 ctcgacctcg cgcagctgcg caaggagctc ggcggcgcgc tcgagggcta ccgcacccag
      961 gcgcaggatc tgtccggcaa gcgccacaag gcggcgcgcg ccctggcgag ggacatcacg
     1021 acacgcatgc agacgctcgg catgacgggc gggcgcttcg aagccgacgt cacccaggac
     1081 ggcaacgccg atccgaccga gcacggcatc gaccggatcg agttccgcgt caccgccaac
     1141 cccggtcagc cgccgcgcgc gctggccaag gtggcctcgg gcggggagct ctcgcgcctc
     1201 tcgctcgcgg tgcaggtgtc gtgcgcggcg cagaacatgc gctgcatggt gttcgatgag
     1261 gtggattccg gcatcggcgg tgcggtcgcg gaaatcgtcg ggcgcgagct gcgtaagctc
     1321 ggcgagcgcg gccaggtgct gtgcgtgacg cacctgccgc aggtcgcctg ccaggcgcac
     1381 caccatctgc gtgtcaccaa gctcaccgac gggcgcacca cccgcacgac gctcaacgag
     1441 ctcatcccgg gcgagcgtgt cgaggagctg gcgcgcatgc tgggcggcgt cgaggtcacg
     1501 gacaaggcgc gcgagcacgc gcgcgacatg ttgaacgccg ccgagcgcgg cggcgccgtg
     1561 ccggtcacgg cgagcgtgag ctcgcgtccg ccggccgccc ggcgcaagta gtcacggccg
     1621 agagccggcg cacagagggc ggttcagctc gtcttgtgct tgcggtacgg gcagttcggg
     1681 cgctcgcagt gcccgtagag aatcagcgaa tgatcgcgga tcgtgaagcc gagcttgtgg
     1741 gcgaccgcgg tctggcgcga ctcgattccc gagtccatga actcctcgac gcgcccgcag
     1801 tcgagacaca cgatgtggtc gtggtgcgtg ccctggttga gctcgaacac cgccatgccg
     1861 tcctcgaagt gatgacgcgt gacgagccca gcggcctcga actgcgtcag cacccgatac
     1921 accgtggcga gcccgatgtc ctcgttcgac tcgagcagca tgcggtagat gtcctcagcc
     1981 gagagatgcc gggtggtgct gccttcgagg atctccagga tcttcaggcg cggcagcgtc
     2041 accttcaggc cggctttgcg caggtccttg ccttccacgg gggttctccc gcgaaccgtc
     2101 acccgcgctt cctggcggac gattcgcaat cgggtatcat cgtttccccc gcgattatcc
     2161 cccatcggac ccgcatgcgc ctaccctcga tcgctgtgac cgccctgctg ctcgcggcgc
     2221 tgccgcttag cggctgcatt tatcgcatga acatccagca ggggaactat ctcgagggca
     2281 agacggttgc tcagctgcag gtcggcatga cgcgcagcca ggtgcgctac ctgctcggct
     2341 cgccgatggt gccggacatc ttcgacaagg accgctggga ttacctctac tacttcaaga
     2401 gcggccacct gcagcggccc gagcagcgcc acctggtcgt gctcttcaag gaagacaagg
     2461 tcgcgagctt ccagcacgac catattcccg atagcgcgcc gcgcgcgccg gacaacgata
     2521 tctcgatcca gaagttcccg aagatctagc ggcccttggt tgacctgtga cgctcgcgct
     2581 gcacgcgctc gcggcggcgg gcgcgcggat cggctcgcag cggccgatac agctcgatgc
     2641 gatcgccatc ctccacgcgc gcagcgcgcg tgcgcagctc gccgaacacg ccgaccggag
     2701 cgaggtccca gagtgcgggg tcgaggccgg cggactcgcg ctccgcggcg cgccgcgccg
     2761 cggcgagcgc gtcggcgatg gtcgcctcac ccggcagctc gacgctccac aggtactgcc
     2821 gggcgggcgt tgcgtaggcg accacgcagc gcttcatgtg gagtcccgtg cccgcgcgac
     2881 gaaggcatcg accagcgagc cgacggtctc ggcgaaccgc ggctcgaaca ggagtcccgt
     2941 gagcgcattc ctgaacgcga agcgcatcgt gagctccacg cggcagccgc cctcgaccgg
     3001 tgtcagcagc caccggccct cgagcgcgcg gaacggcccg ctgacgagcc gcatctcaac
//
LOCUS       DATLKL010000056         1719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_68336, whole genome shotgun sequence.
ACCESSION   DATLKL010000056 DATLKL010000000
VERSION     DATLKL010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68336"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VM713_00775"
     CDS             complement(<1..257)
                     /locus_tag="VM713_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HVH82825.1"
                     /translation="MGKQIKGILGTKLGMTQVFNEAGQIVPVTVVQAGPCVVSAVRTP
                     DRDGYSAVQLGFGEIDPRKVTKPVSGMFEKAGLTPRRYVAEL"
     gene            complement(271..579)
                     /gene="rpsJ"
                     /locus_tag="VM713_00780"
     CDS             complement(271..579)
                     /gene="rpsJ"
                     /locus_tag="VM713_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012578474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HVH82826.1"
                     /translation="MAGQKIRIRLKAYDHEVIDSSARKIVDTVTRTGARVAGPVPLPT
                     EKNVYCVIRSPHKYKDSREHFEMRTHKRLIDIIDPTPKTVDSLMRLDLPAGVDIEIKL
                     "
     gene            complement(693..>1719)
                     /gene="tuf"
                     /locus_tag="VM713_00785"
     CDS             complement(693..>1719)
                     /gene="tuf"
                     /locus_tag="VM713_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HVH82827.1"
                     /translation="RDETKTVTIAVSHVEYESDKRHYAHIDCPGHADYIKNMITGAAQ
                     MDGAILVVSAADGPMPQTREHILLARQVEVPFIVVALNKADMVDDPELLELVQLEVRE
                     LLSRYNFPGDDIPVVEVSALKALEGDAEWSNKVLELLNACDESIPEPVREVDKPFLMP
                     VEDVFSITGRGTVVTGRIERGTIHTSGSVEIIGIKSKAQTTVVTGVEMFRKILDDGQA
                     GDNVGLLLRGTKREEVERGQVVIKPGTNTPHTEFEGQVYILSKDEGGRHTPFFNNYRP
                     QFYFRTTDVTGVVNLPEGTEMVLPGDRTDMRVELIQPIAMEEGLRFAIREGGRTVGAG
                     RVTKIIK"
BASE COUNT          236 a          564 c          579 g          340 t
ORIGIN      
        1 agctcggcca cgtacctgcg cggcgtcagg ccggccttct cgaacatccc gctgaccggc
       61 ttggtcacct tccgggggtc gatctccccg aagccaagct gcacggcgga gtacccgtcg
      121 cggtccggcg tgcgcacggc ggataccacg cagggcccgg cctgcacaac cgtgaccggg
      181 acgatctgcc cggcctcgtt gaagacctgg gtcatgccga gcttggtgcc caggatcccc
      241 ttgatttgct tgcccatgtc ggtgcgttcc tcagagcttg atctcgatgt cgacgccggc
      301 cggcaggtcg agccgcatca gcgagtccac cgtcttgggc gtggggtcga tgatgtcgat
      361 cagcctcttg tgcgtccgca tctcgaagtg ctcgcgcgag tccttgtact tgtgcggaga
      421 gcggatgacg cagtacacgt tcttctcggt aggcagcggc accgggccag ccacccgcgc
      481 gccggtacgc gtcacggtgt cgacgatctt ccgcgccgag ctgtcgatga cctcgtggtc
      541 atatgccttg agccggatgc ggatcttctg tcccgccatg ttggcctcgg tgtccttcgc
      601 ttcttagtca gccgctgttc gaacgttggt cggcgtgccg cggcggccgg gtcccgttga
      661 accggtgagc ccggccgccg cgattacgac cgttacttga tgatcttcgt gacccggccg
      721 gcgccgacgg tgcggccgcc ctcgcggatc gcgaaccgca ggccttcctc catggcaatc
      781 ggctggatca gctcgacgcg catgtcggtc cggtcgcccg gcaggaccat ctcggtgccc
      841 tcggggaggt tcaccacgcc ggtcacatcg gtggtccgga agtagaactg cggacggtag
      901 ttgttgaaga acggcgtgtg ccgaccgccc tcgtccttgg acaggatgta gacctggccc
      961 tcgaactcgg tgtgcggggt gttggtgccc ggcttgatca cgacctggcc gcgctccacc
     1021 tcctcgcgct tggtgccgcg cagcagcagg cccacgttgt cgccggcctg gccgtcgtcc
     1081 aggatcttgc ggaacatctc cacgccggtg acgacggtgg tctgcgcctt ggacttgatc
     1141 ccgatgatct cgacggaccc gctggtgtgg atggtgccgc gctcgatccg gccggtcacc
     1201 acggtgccgc ggccggtgat cgagaacacg tcctccaccg gcatcaggaa cggcttgtcg
     1261 acctcgcgca ccggctccgg gatggactcg tcacaggcgt tcaggagctc aaggaccttg
     1321 ttgctccact cggcgtcgcc ctcgagcgcc ttcagcgcgg agacctcgac gaccgggatg
     1381 tcgtcgccgg ggaagttgta gcgcgagagc agctcgcgca cctcgagctg gacgagctcg
     1441 agcagctcgg ggtcgtcgac catgtccgcc ttgttgaggg cgacgacgat gaaggggacc
     1501 tcgacctggc gggcgagcag gatgtgctcc cgcgtctgcg gcatcggccc gtcggcggcg
     1561 ctgacgacga ggatggcccc gtccatctgt gctgcgccgg tgatcatgtt cttgatgtag
     1621 tcggcgtgcc ctggacagtc gatgtgggcg tagtgccgct tgtctgactc gtattcgacg
     1681 tggctgacgg cgatcgtgac cgtcttggtt tcgtcgcga
//
LOCUS       DATLKL010000057         2546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_34514, whole genome shotgun sequence.
ACCESSION   DATLKL010000057 DATLKL010000000
VERSION     DATLKL010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34514"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..>2546
                     /locus_tag="VM713_00790"
     CDS             133..>2546
                     /locus_tag="VM713_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82828.1"
                     /translation="MRAVRNLRRLLLLASACLLVLVIVVVPAAITGAAVFTESGHQFL
                     VRHTPERFGSGPGAVRLTITGVSGTLARGVHADRVEIDHELVHLTFEGIDGRVALAPL
                     LLQTIRSPNTTVHSALIEVKHRTRQVPPSPPVFLPRWLLISAEHTIVDDAVLKIYNGA
                     RLEATHITAAAVLRHRSIRFFQAQGNFGDARVDAIGELRARDPLGIEAQGHVEWTPAG
                     QPAWRIDGSARGDLNALNLTAHALSPFRADVTGRALDLTSHWHMVGDANVENFDLRAW
                     GVSGPLGVMKARLALSWNSEGFSGRGQVDPSGLHVGTFDAEFDGSFAAHVLTAKHMEA
                     RHPSGAHAVGSGTFAIVPNGPRLDLSGSWEDFRWPLVGPEAIVKSAAGSFTLQGLLPY
                     KVHAQGAASAAGISASTVDLTGTLGKDSFAFERAELDLLEGHASLAGAVAWAPRPSWS
                     VAGQLTGINPAALRADLPGSVSGALAVSGNGFDPRGDLRITAGNLSGKLRGARVSGGG
                     SINRAGGTWSFDRVRVALGGTSLALDGRASEQLDLRFALASSDLALLAPGSHGQLNTS
                     GTLRGTTTDPAIVANAHASGLSYAGLKLEHLDADIDFNAADRERPTKIDARLTNLSYA
                     SHTVESAAVTVTGPPAAYRVHAEARAAGLALAAEAAGAYARGMFGGQLTALTLKGKDA
                     LDLALEHPVDLTASAKQLRLEWLCLAGTPGSMCLDADWSGERWSATLMSRQLPLSALT
                     AGMTPAVQYQGTVDLLLRAAGGLQTPSTGTLRAQLTDAVLSHRLLSKRIEHTRIGSGT
                     VTASATP"
BASE COUNT          368 a          940 c          872 g          366 t
ORIGIN      
        1 caagctcttc tattccgcgg gcgtcggcat ccgcttccgc acgccggcgc tgacgctcgg
       61 catcgacgtg gcccagccca tcaactcgcc cggctcccgc ccgcggctgc acatcaactt
      121 ctcgccgaag ctgtgagagc cgtccgcaac ctgcgccgcc tgctgctcct cgcgagcgcc
      181 tgcctgctgg tcctcgtgat cgtggtggtg ccggccgcga tcaccggcgc cgcggtgttc
      241 accgaatcgg ggcaccagtt cctggtgcgg cacacacctg agcgcttcgg ctcgggtccc
      301 ggcgcggtgc gcctcacgat cacgggtgtc agcggcacgc tcgcgcgcgg cgtgcacgcc
      361 gaccgcgtcg agatcgatca cgagctggtg cacctcacgt tcgagggcat cgatggccgc
      421 gtggcgctcg ccccgctcct gctgcagacc atccgctcgc cgaataccac ggtgcacagc
      481 gcgctcatcg aggtgaagca ccgcacccgg caggtgccgc cctcgccgcc ggtgttcctg
      541 ccgcgctggc tgctgatcag cgccgagcac accatcgtcg acgacgcggt gctgaagatc
      601 tacaacggcg cgcgtctcga ggccacccat atcactgccg ccgcggtgct gcgtcaccgc
      661 tcgatccgtt tcttccaggc gcagggcaac ttcggcgacg cccgcgtcga tgctatcggc
      721 gagctgcgcg cccgcgaccc tctcggcatc gaggctcagg ggcacgtcga gtggacgccg
      781 gccggacagc cggcctggcg catcgacggc tcggccaggg gcgatctcaa cgcgctcaat
      841 ctgacggcgc acgccttgag cccgttccgc gccgacgtca ccggccgggc actcgacctc
      901 accagccatt ggcacatggt aggcgacgcc aacgtggaga acttcgacct gcgcgcctgg
      961 ggtgtctcgg ggccgctcgg cgtgatgaag gcgcgcctcg cgctcagctg gaacagcgag
     1021 ggtttcagcg gccgcggcca ggtcgacccg agcgggctcc atgtcggcac gttcgacgcc
     1081 gagttcgacg gctccttcgc cgcgcacgtg ctcacggcga agcacatgga ggcacgccac
     1141 ccctcgggag cacacgcggt cggctccggc accttcgcca tcgttcccaa cggaccgcgg
     1201 ctcgatttga gcggcagctg ggaagacttc cgctggccgc tggtggggcc agaggccatc
     1261 gtgaagagcg cggcgggctc gttcaccctg cagggactgc tgccctacaa ggtgcacgcg
     1321 cagggtgcgg cgagcgcggc cgggatctcc gccagcaccg tggacctgac cggcaccctc
     1381 ggaaaggaca gcttcgcctt cgagcgcgcc gagctcgacc tgctcgaggg tcacgcgagc
     1441 ctcgctggcg cggtagcctg ggcgccgcgc ccgagctggt ccgtcgccgg ccagctcacc
     1501 ggcatcaatc cggcggcgct gcgcgccgat cttccaggca gcgtcagtgg cgcgctggcg
     1561 gtgtccggca acggcttcga cccgcgcggc gacctgagaa tcacggccgg gaacttgagc
     1621 ggcaagctgc gtggggcgcg ggtgagcggc gggggctcga tcaatcgcgc cggcgggacc
     1681 tggagtttcg accgggtgcg cgtggcgctg ggcggcacga gcctcgccct cgatggtcgc
     1741 gccagcgagc aactcgacct gcgcttcgcg ctcgcgagct cggacctcgc gctgctcgcg
     1801 cccggcagtc acgggcagct caacaccagc ggcacgctgc gcgggacgac caccgacccg
     1861 gcgatcgtgg cgaacgccca cgcgagcgga ctcagctacg cgggactcaa actcgagcac
     1921 ctcgatgccg acatcgactt caacgcggcc gaccgcgagc ggccgaccaa gattgacgcg
     1981 cggctgacga atctgagcta cgcgagccat accgtcgaga gcgcggccgt cacggtcacc
     2041 ggccctccgg cggcttaccg ggtgcacgcg gaagcccgcg ccgcgggcct ggcgctcgcg
     2101 gccgaggcgg ccggcgcgta cgcacgcggg atgtttggcg gccagcttac cgccctcact
     2161 ctcaagggca aggacgcgct cgatctcgcc ctcgagcatc ccgtcgatct cactgcctcg
     2221 gcgaagcagc tgcggctcga gtggctgtgt ctcgcgggta cgcctggcag catgtgcctt
     2281 gatgcggact ggagtggcga gcgctggtcc gcgacgctga tgagcaggca gctgcccttg
     2341 agcgcactca cggccggcat gacgcccgcg gtgcagtacc agggcacggt cgacctgctg
     2401 ctgcgcgcgg cgggcggctt gcagacgcca tccaccggca ccttgcgcgc gcagctcacc
     2461 gacgcggtgc tctcgcaccg gctcctcagc aagagaatcg agcacacgcg catcggctcg
     2521 ggcaccgtga cggcgagcgc cacgcc
//
LOCUS       DATLKL010000058         2091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_203434, whole genome shotgun sequence.
ACCESSION   DATLKL010000058 DATLKL010000000
VERSION     DATLKL010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203434"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2091
                     /gene="acs"
                     /locus_tag="VM713_00795"
     CDS             <1..>2091
                     /gene="acs"
                     /locus_tag="VM713_00795"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HVH82829.1"
                     /translation="LNEQRTFPPSPQFTARARIGPKDLESLRRRAAEDHVGFWADLAS
                     QELSWHQPFTVALDEARAPNYRWFTDGRINASWNCLDAHLNERGHKTAVIFEGEPGDV
                     RRLSYRELHADVCRFANALKALGVAKGDRVVIYMPMVPEVLVAMHACNRIGAIHSVVF
                     GGFSAPALKDRIEDTEAKLVITADGGWRGGAAVELKSNTDHALAAGCPTGKRVIVLKR
                     TGRPVPMDTQRDLWWHEAIEGQPPSYEADWVEAEHPLFILYTSGSTGKPKGIQHATAG
                     YLLGAKVTTQWVFDLRDDDVFWCTADVGWVTGHTYIAYGPLAAGATVLMYEGAPTVPD
                     GGRFWKNCQAHGVTVFYTAPTAIRALMKLGDELPGKYDLSKLRLLGSVGEPINPEAWM
                     WYHRVIGKERCPIVDTWWQTETGAILISPLPGVTATKPGSCTQPLPGIDADIVNDHGE
                     SVKRADAGGYLVIRKPWPSMLRTIWRNNPRYVEGYWSKFHSRLYVAGDSAYRDKDGYF
                     WIMGRIDDVLKVSGHRLGTMEIESALVAHPRVAEAAVVGKPHEIKGESVFAYVVLRGP
                     RPTGDTSALTKELRDWVGEQLGPIAKPDDIRFADNLPKTRSGKIMRRLLKAIARGEEI
                     TQDVSTLENPAILEQLRGTDQRAGAAAPAKAKAAARPRPAAKSAGRRATRKRIKAAAA
                     RRPRSKPSGARKASR"
BASE COUNT          362 a          727 c          694 g          308 t
ORIGIN      
        1 cctcaacgag cagcgcactt tcccgccctc gccgcagttc acggcgcgcg cccgcatcgg
       61 accgaaggac ctcgagagcc tgcgccgccg tgcggccgaa gatcatgtcg gattctgggc
      121 ggaccttgcc tcgcaggagc tcagctggca ccagccgttc accgtggcac tcgatgaggc
      181 gcgcgcgccc aactaccgct ggttcaccga cgggcgtatc aacgcctcgt ggaactgcct
      241 cgacgcgcac ctgaacgagc gcggccacaa gaccgcggtc atcttcgagg gcgagccggg
      301 ggatgtgcgg cggctctcct atcgcgagct gcacgccgac gtctgccgct tcgccaatgc
      361 gctcaaggct ctgggtgtcg ccaagggcga ccgggtcgtc atctacatgc cgatggtgcc
      421 cgaggtgctc gtggcgatgc atgcctgcaa ccgcatcggc gcgatccact ccgtagtgtt
      481 cggcggcttt tcggcgcccg cgctcaagga ccgcatcgag gacaccgagg ccaagctcgt
      541 cattacggcc gacggcggct ggcgcggcgg tgcggcggtg gagctcaagt ccaacaccga
      601 tcacgcgctc gccgccgggt gcccgacggg caagcgggtc atcgtgctca agcgtaccgg
      661 gcggccggtc cccatggaca cgcagcgcga cctgtggtgg cacgaggcca tcgaggggca
      721 gccgccgtcg tacgaggccg actgggtcga agcggagcat ccgctcttca tcctctatac
      781 ctcgggctct acgggtaagc ccaagggcat tcagcacgcg accgccggct atctgctcgg
      841 cgccaaagtc acgacgcaat gggtgttcga tctgcgcgac gacgatgtgt tctggtgcac
      901 ggcggatgtc ggctgggtca ccggacacac ctacatcgcc tacggaccgc tcgccgcggg
      961 cgcgaccgtc ctcatgtacg agggcgcccc cacggtgccc gacgggggcc gcttctggaa
     1021 gaactgccag gcgcacggcg tcaccgtctt ttacaccgcg ccgaccgcga tccgcgcgct
     1081 gatgaagctc ggcgacgagc tgccggggaa gtacgactta tcgaagctgc gcctcctcgg
     1141 cagcgtcggc gagccgatca accccgaggc gtggatgtgg tatcaccgcg tgatcggcaa
     1201 ggagcgctgc ccgatcgtcg acacctggtg gcagaccgag accggcgcga tcctgatctc
     1261 accactcccc ggcgtgacgg cgaccaagcc cggctcatgt acccagcccc tgccgggcat
     1321 cgatgccgat atcgtcaacg atcacggcga gtcggtgaag cgcgccgacg ccggcggcta
     1381 tctcgtgatc cgcaagccct ggccgtcgat gctgcgcacc atctggcgca acaacccgcg
     1441 ctacgtcgag ggctactggt cgaagttcca cagccgcttg tacgtggcgg gcgacagcgc
     1501 gtaccgcgac aaggacggtt acttctggat catgggccgc atcgacgacg tgctcaaagt
     1561 gtccgggcac cgtctcggta ccatggagat cgaatcggcg ctggtcgcgc atccgcgcgt
     1621 cgccgaggca gccgtggtcg gcaagcctca cgagatcaag ggggaatcgg tgttcgcgta
     1681 cgtcgtgctg cgcggcccgc gtcccaccgg ggacacgagc gcgctcacca aggagctgcg
     1741 cgactgggtg ggggagcagc tgggtccgat cgccaagccc gacgacatcc gttttgccga
     1801 caatcttccc aagactcgct cgggcaagat catgcgccgg ctgctgaaag ccattgcgcg
     1861 cggcgaggag attactcagg acgtctcgac gctggagaat cccgccatcc tcgagcagtt
     1921 gcgcggcacc gatcagcgcg cgggcgccgc agcgccagcc aaggcaaaag ccgcagccag
     1981 acccaggcca gcagccaaga gcgcagggcg cagggccacg cgcaagcgca tcaaagcggc
     2041 tgcggcgcgc cgcccgcgca gcaagccttc cggagcccgc aaggcctcac g
//
LOCUS       DATLKL010000059         1621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_124726, whole genome shotgun sequence.
ACCESSION   DATLKL010000059 DATLKL010000000
VERSION     DATLKL010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124726"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VM713_00800"
     CDS             <1..574
                     /locus_tag="VM713_00800"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HVH82830.1"
                     /translation="LVRAQIAAALATRRPAAVKIGMLGSRAIIEAVADLLPPRELVPL
                     VLDPVLAATSGGTLLDAAGRAALIALLLPRTTLLTPNIPEAAALLETAPAESAAEIDA
                     QGRALCARGAAAVLVKGGHAAGANATDRLIRHDGSVREFTAPRLAAARRGTGCALASA
                     IAAGLARQLPLELACQRAKEHVTELLQQPA"
     gene            646..>1621
                     /locus_tag="VM713_00805"
     CDS             646..>1621
                     /locus_tag="VM713_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HVH82831.1"
                     /translation="MSPQPDRQDELSALARRAMREHGLEPDFPPAALAQLRAIAAPAA
                     ATGPDVRDLRALAWCSIDNDDSRDLDQLSVAEVLAGGATRVQVAIADVDATVAAGSPL
                     DAHASANTTSVYTAAQVFPMLPERLSTDLTSLADGQERTSLVIEMNVTADGSVSSSDV
                     YRARVVNRAKLAYNSVAAWLDGKGPMPPPVAAVKGMDQQLRIQDRVAQALKRVRKAQG
                     ALGLTTLEAQAVYEGGALMDLKPDEDNRAKELIEYFMIAANGVVAQFLEGKRSPSLRR
                     VLRVPERWGRIVALAATVGGALPAAPDARALSDFLVQCQQRSPERFPDL"
BASE COUNT          229 a          602 c          568 g          222 t
ORIGIN      
        1 gctggtgcgg gcgcagatcg ccgcggcgct ggcgacacgg cgccccgccg ccgtcaagat
       61 cggcatgctc ggaagccggg cgatcatcga ggccgtggcc gatctcctgc cgccgcgcga
      121 gctcgtgccg ctggtgctcg atcccgtgct cgcggccacc tcaggcggca cgctgctcga
      181 cgctgcaggc cgtgccgctc tcattgcact gctcctgccg cggacgacac tgctcactcc
      241 taacatcccg gaagcggcag cactgctgga gaccgcgccg gctgagtcgg cggcggagat
      301 cgacgcacaa gggcgggcgc tgtgcgcacg cggcgcggcc gcggtgctcg tgaagggcgg
      361 acacgcggcc ggtgcgaatg ccaccgatcg gctcatcagg cacgacggga gcgtgcgcga
      421 attcaccgcg ccgcgcttgg cggctgcccg acgcggcacg ggctgcgcgc tggcgtcggc
      481 gatcgcggcc gggctcgccc ggcaactgcc gctcgagctg gcctgtcagc gagccaagga
      541 gcatgtgacg gagctgttgc aacagccggc ctgagctcgc cagtcgtagc agcacgggcg
      601 ccacgcgcgc gcggcgcgct tgctttttgc gggtatcatc cccggatgtc acctcaacct
      661 gatcggcagg acgagctgtc cgccctcgcg cggcgcgcga tgcgcgagca tggcctggag
      721 cccgacttcc caccggccgc cctcgcccag ctgcgcgcca tcgccgcccc ggcggccgca
      781 accgggcccg acgtgcgtga tctgcgcgcg ctggcgtggt gctcgatcga caacgacgat
      841 tcacgcgacc tcgaccagct gtcggtcgcc gaagtgctgg cgggcggcgc cacgcgcgtg
      901 caggtggcca tcgccgacgt cgacgccacc gtggccgccg gctcgccgct cgatgcgcac
      961 gccagcgcca acacgaccag cgtctacacg gccgcgcagg tatttccgat gctgccggag
     1021 cggctctcga ccgacctgac gtcgcttgcc gacggacagg agcgcacgag cctcgtcatc
     1081 gagatgaacg tgacggccga cgggtccgtg agctcctccg acgtctatcg cgcgcgggtc
     1141 gtcaaccgcg ccaagctcgc ctacaacagc gtggccgcct ggctcgacgg caaggggccc
     1201 atgccgccgc cggtagcggc ggtcaaggga atggaccagc agctgcgcat ccaggaccgg
     1261 gtcgcgcagg ccctgaagcg cgtccgcaag gcgcagggcg cgctcggcct caccacgctc
     1321 gaagcgcagg cggtctacga gggcggggcg ctcatggacc tgaagcccga cgaggacaac
     1381 cgcgccaagg agctcatcga gtatttcatg atcgcagcca acggcgtggt cgcgcagttc
     1441 ctcgaaggca agcgctcccc ctcgctccgc cgcgtcctgc gtgtgcccga gcgctgggga
     1501 cgcatcgtgg cgctcgccgc taccgtcggc ggggcgctcc ccgcggcgcc cgatgcgcgc
     1561 gccctgagtg acttcctcgt gcagtgccag cagcgttcgc ccgagcgctt ccctgacctg
     1621 t
//
LOCUS       DATLKL010000060         1967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_68390, whole genome shotgun sequence.
ACCESSION   DATLKL010000060 DATLKL010000000
VERSION     DATLKL010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68390"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VM713_00810"
     CDS             <1..188
                     /locus_tag="VM713_00810"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HVH82832.1"
                     /translation="LSLFAVLPGPMSPPEAFEELVSTARNLNDRLCGVLQDEAGTPLT
                     PARIASLRERLSGEALS"
     gene            185..>1967
                     /gene="ligA"
                     /locus_tag="VM713_00815"
     CDS             185..>1967
                     /gene="ligA"
                     /locus_tag="VM713_00815"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HVH82833.1"
                     /translation="MTPGPAARAAELREQLKQHDYRYYVLDDPTVPDAEYDRLMAELR
                     ALEAAHPELVTPDSPTQRVSGAPSAAFGEVVHAVPMLSLDNAFSEEDVAAFDRRIHER
                     LGTHGELDYVAEPKLDGLAIAVLYRAGQLKQAATRGDGVTGEDVTANMRTLRSVPHRL
                     LGDAAPLIEVRGEVFMPLKGFERMNARARERGEKVFVNPRNAAAGSLRQLDPRVSASR
                     PLDAFFYGLGALEGAPLPKGQAELLAWLRSLGLPVSPDAHPVRGVAGCLSYYRGMGAR
                     RAALPYQIDGVVYKPESRADQERLGFISRAPRWTIAHKFPAEEALTLVRGVEFNVGRT
                     GALTPIARLEPVFVGGVTVSNVTLHNIDEVHRKDVRVGDTVVVRRAGDVIPELVSVVL
                     DKRPMSPARPGEPQSPQVELPARCPVCDSLVLRVEGEAVARCTGGFTCRAQRQEALRH
                     FASRRALDIEGLGDKVVEQLVERQLVRSPADLYTLTVAQLAELERMGEKSAENLVAAI
                     HKSRETTLPRLLYELGIREVGEATALALARHFGTLEALMKASAEEIQQVEDVGPVVAA
                     HVATFFASDDHINVIKALRKEGVKWPEV"
BASE COUNT          291 a          694 c          697 g          285 t
ORIGIN      
        1 ccctgtcgct gttcgcagtg ctcccgggac cgatgtcgcc gcccgaagcg ttcgaggagc
       61 tggtgagcac cgcgcgcaac ctcaacgacc ggctgtgcgg cgtgctgcag gatgaggctg
      121 gcacaccgct cacaccggcg cgcatcgcca gcctgcgcga gcggctctcg ggcgaggcgc
      181 tctcgtgaca cccggcccgg cggcgcgcgc cgccgagttg cgcgagcagc tcaagcagca
      241 cgattaccgc tactacgtcc tcgacgatcc gacggtcccg gacgccgagt acgaccgcct
      301 gatggccgag ctgcgcgcgc tcgaggcggc gcaccccgag ctcgtcacac ccgactcgcc
      361 gacccagcgc gtgtccggag cgccgagcgc cgccttcggc gaggtcgtgc acgcagttcc
      421 gatgctctcc ctcgacaacg ccttcagcga agaggacgtc gcggccttcg atcgccgcat
      481 acacgagcgc ctcggaacgc acggcgagct cgactatgtc gccgagccga agctcgatgg
      541 gcttgcgatc gcggtgctct atcgcgcggg tcagctcaag caggccgcga cccgcggcga
      601 cggcgtcacc ggcgaggacg taaccgccaa catgcgcacc ctgcgcagcg tgccgcaccg
      661 actgcttggg gacgcggcgc cgctcatcga agtgcgcggc gaggtgttca tgcccttgaa
      721 gggcttcgag cgcatgaacg cacgggcgcg cgagcgtggc gagaaggtgt tcgtcaatcc
      781 gcgcaacgcc gccgccggga gcctgcgcca gctcgatccg cgcgtgagcg cctcgcgccc
      841 cctcgatgcc tttttctacg ggctcggcgc cctcgagggt gcgccgctcc ccaaaggaca
      901 ggccgagctc ctcgcctggc tgcgctcgct cgggctgccg gtgtcgccgg atgcacaccc
      961 ggtgcgcggc gtcgccgggt gcctctccta ctaccgcggg atgggcgcgc gccgcgccgc
     1021 gctgccgtac cagatcgacg gcgtcgtcta caagcccgag tcgcgcgccg accaggagcg
     1081 cctcggcttc atctcgcggg cgccgcgctg gacgatcgcc cacaagttcc cggcggagga
     1141 ggcgctcact ctcgtgcggg gcgtcgagtt caacgtgggc cgcaccggcg cgctcacgcc
     1201 catcgcgcgc ctcgagccgg tgttcgtcgg cggcgtgacc gtgagcaacg tgacgctgca
     1261 caacatcgac gaggtgcatc gcaaggacgt gcgggtcggc gatacggtcg tggtgcggcg
     1321 cgcgggggac gtgatcccgg agctggtgag cgtcgtcctc gacaagcggc cgatgagccc
     1381 tgcgcggccg ggcgagccgc agtcgccgca ggtggagctg ccggcgcgct gcccggtgtg
     1441 cgactcgctc gtgctgcgcg tcgagggcga ggcggtggcg cgctgcaccg gaggcttcac
     1501 ctgccgcgcg cagcgccagg aggctctgcg gcactttgcc agccgccggg cgctcgacat
     1561 cgagggcttg ggcgacaagg tggtcgagca gctcgtggag cgccagctgg tgcgctcgcc
     1621 ggccgatctc tacacgctca cggtggcgca gctcgccgag ctcgagcgca tgggcgagaa
     1681 atctgccgag aatctcgtcg ctgcgatcca taaatccagg gagacgacgc tgccgcgcct
     1741 gctctacgag ctcggcatcc gcgaagtggg cgaggccacc gcgctcgcgc tggcgcgcca
     1801 tttcggcacg ctggaagcgc tcatgaaggc gagcgccgag gagatccagc aggtcgagga
     1861 cgtcggcccg gtggtagcgg cgcacgtcgc gaccttcttt gcctcagacg atcacattaa
     1921 cgttatcaag gcattacgca aggaaggtgt gaagtggcct gaagtcg
//
LOCUS       DATLKL010000061         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_124750, whole genome shotgun sequence.
ACCESSION   DATLKL010000061 DATLKL010000000
VERSION     DATLKL010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124750"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..401)
                     /gene="fis"
                     /locus_tag="VM713_00820"
     CDS             complement(108..401)
                     /gene="fis"
                     /locus_tag="VM713_00820"
                     /inference="COORDINATES: protein motif:HMM:NF001659.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HVH82834.1"
                     /translation="MTAKKKTRTREAAAARVNGRDLPLRNHAERALSEYFTSLNGHRP
                     AHLYDLVLREVEEPLFRVVLDYAQGNQSRAAGILGINRGTLRKKLKQLGLAGS"
     gene            complement(428..1492)
                     /gene="dusB"
                     /locus_tag="VM713_00825"
     CDS             complement(428..1492)
                     /gene="dusB"
                     /locus_tag="VM713_00825"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HVH82835.1"
                     /translation="MNCSGSAHPCAAPRAASQKRGFGSQVRIGPHVLPSPVVLAPMAG
                     VTDRPFRILCRRLGAGLAASEMLTSDTRLWTTAKSRHRMDHEGEPEPRVVQLAGADPR
                     ALAEAARLNVALGAQIIDLNMGCPAKKVCGQLCGSALLRDEPLVARILEAVVRAVDVP
                     VTLKIRTGWDRESRNGVNVARLAERCGIAALAVHGRTRADFYAGSAEYATIRDIKTHV
                     RIPVFANGDINDGRKAREVLDFTGADGVMLGRASHGSPWIFRAVNRELNSELVTPTLQ
                     RRDLRDIILAHLDSVYGFYGEETGVRIARKHLGWYCGQLLSEPATVRRDLMGAASTAA
                     QFAQALKHFDEWVGEAAVAA"
     gene            complement(1495..>2094)
                     /locus_tag="VM713_00830"
     CDS             complement(1495..>2094)
                     /locus_tag="VM713_00830"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HVH82836.1"
                     /translation="ELAEARPEDTDVGGRALFEAAGRPQPLARGTLAWAAGSALLVLV
                     LVAQIIDHDRDTLATDPRFSRALTAIYSALGVTLMPRWDLRAYDVRQLGATVGQGSLG
                     AITVRASIRNSGPIPQPLPLLRVTLQDRFGNRLASRDVQPRDYLPQSAAQTALLTRDQ
                     RIDAELAFVDPGANAVGFELDACLPMNGGIACANDTSAR"
BASE COUNT          336 a          698 c          725 g          335 t
ORIGIN      
        1 gtcttgtcgg agacggatag cagcgcgcgg cgcacggctg gcgacgacat gggcttcagc
       61 ccctcatggc ggccggcgcg cgctggcgcc ggcgggaatc agcggcttca ggagccggca
      121 agtccgagct gcttcagctt cttgcgcagc gttccacgat tgatgccgag gatgccggcg
      181 gcgcggctct ggttgccctg cgcgtagtcg agcaccaccc gaaacagggg ctcctccact
      241 tcgcgcagca ccaggtcgta cagatgggcg ggtcggtggc cgttcaggct ggtgaagtac
      301 tcgctgagcg cacgctcggc atggttacgc agcggcagat cgcgtccatt cactctggcg
      361 gcggcggctt cacgagtccg ggtcttcttt tttgccgtca tgtgatcccc cagggaaaaa
      421 ctgttcgtca ggccgccacg gcggcctccc ccacccattc gtcgaaatgc ttaagcgcct
      481 gcgcgaactg tgcggcggta ctcgcggccc ccatcagatc ccgccggacc gtcgccggct
      541 cactcagcag ctgcccgcaa taccagccga gatgtttgcg cgcgatccgg actccggtct
      601 cctcgccgta gaagccgtac accgagtcga gatgcgcgag gataatatca cgcaggtcgc
      661 ggcgctgaag tgtcggcgtc acaagttcgc tgttcagctc gcgattgaca gcgcgaaaaa
      721 tccacggcga cccgtggctc gcgcgaccga gcattactcc gtctgcaccg gtgaaatcaa
      781 gtacctcgcg ggcttttctt ccgtcattga tgtcgccgtt tgcgaacact ggaatgcgaa
      841 cgtgcgtctt gatgtcgcga attgtcgcgt actcggccga gccggcgtag aaatccgcgc
      901 gcgtgcgccc gtgcaccgcg agcgctgcga tgccgcagcg ctccgcgagg cgtgcaacgt
      961 tcaccccatt gcgagactcg cgatcccagc cggtgcggat cttgagcgtc acgggcacat
     1021 ccacggcacg caccacggct tcgagaatgc gcgccaccag cggctcgtcg cggagcagtg
     1081 ccgatccaca gagctggccg cagacttttt tcgccggaca gcccatgttc agatcgatga
     1141 tctgcgcgcc gagcgcgacg ttgaggcggg ctgcttcggc cagcgcgcgc gggtcggcgc
     1201 cggcgagctg caccacgcgc ggctcgggct cgccctcgtg gtccatacgg tggcgcgact
     1261 tcgcagtcgt ccacagacgc gtgtccgagg tgagcatctc tgaggccgcg agccctgcgc
     1321 cgagccggcg gcagagaatg cggaacgggc ggtcggtcac cccggccatg ggcgcgagca
     1381 cgacgggcga gggcaggacg tgaggtccga tccgcacttg cgagccaaaa ccgcgctttt
     1441 ggctcgccgc gcgaggggcg gcacagggat gtgccgatcc cgagcaattc ataatcaacg
     1501 cgcgctcgtg tcgttggcgc aggcgatgcc gccgttcatc ggcaggcagg catcgagctc
     1561 gaagcccacg gcgttcgccc cggggtcgac gaatgcgagc tcggcgtcga tgcgctggtc
     1621 gcgggtcagg agcgccgtct gggcggcgct ctgcggcaga taatcgcgcg gctgcacgtc
     1681 gcgcgaggcg aggcgattgc cgaagcggtc ctgcagcgtc acgcgcagca gcggcagcgg
     1741 ctgaggaatc ggtcccgaat tacggatgct ggcacgcacg gtgatggcgc cgaggctgcc
     1801 ctggcccacg gtggcgccga gctgccgcac gtcgtaggcg cgcaggtccc agcgcggcat
     1861 gagcgtcacc ccgagcgccg aatagatcgc ggtgagcgcg cgactgaagc gcgggtcggt
     1921 ggcgagcgta tcgcggtcat gatcgataat ctgcgcgacc aggacgagca cgagtaacgc
     1981 ggaaccggcg gcccaggcca gagtgccgcg cgcgagcggc tgcggccttc cggcggcctc
     2041 gaacagggcg cggcccccga cgtccgtatc ctccggtcgc gcctcggcga gctc
//
LOCUS       DATLKL010000062         2803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_11902, whole genome shotgun sequence.
ACCESSION   DATLKL010000062 DATLKL010000000
VERSION     DATLKL010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11902"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VM713_00835"
     CDS             complement(<1..190)
                     /locus_tag="VM713_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82837.1"
                     /translation="MRIPLVRGWAPLLAALASWPALAGAPFSFDATPGRLPKEIVPLD
                     YAIAVVPDIDAGTIAGTES"
     gene            complement(202..1989)
                     /locus_tag="VM713_00840"
     CDS             complement(202..1989)
                     /locus_tag="VM713_00840"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HVH82838.1"
                     /translation="MHAPRILVLTATTALAAGAALAQTQHQALVSLAQDITYTTADVF
                     PMTATYLGIPGHDGELHSPGEAFRAAQVQRLREWQRRLEAIAAAFDAHAPLVDRDDAA
                     LLRATLSRSLNELLVYQTDRKDYAGGANSVVDVVFVQFEHLPVVGQEGATAADLKRGW
                     ADIARRLAGAPAYIAASQRLANTPGHLYGVVGARQLAGAPDFLKGALTEAARTQLGVR
                     SREYTAFLRARDTALGTIAQTRQYLEAHAASWPENYAIGRAAYDRMLKEEQLLPYVAD
                     DVERIAQDELAHGWAEEAWLRQLAQQRGVALGAPSGGGLAPGGAALIDYYRDCIAQLR
                     KFVTDEDIVTIPAWLGTMQIVETPAFLQPVSPGASMNPPRLFSLSTTGYYYITPPKSL
                     AEAAARLDMNEDFDRDRILSTAAHEALPGHFMQLSIAKRHTDFVRRVEDSSAFAEGWA
                     FYGEEMFVRLGLYGDDLDGRLAVARWERVRGARAVVDPKLASGEWTYERAVEFFAAET
                     GFTREQAEAAIAAIATGPGYVIAYSVGRLQIENLLAEYLRRKGADASLHDFHDRLLSY
                     GTTPLAIVAPELLADLDKPASAVRAAANY"
     gene            complement(2080..>2803)
                     /locus_tag="VM713_00845"
     CDS             complement(2080..>2803)
                     /locus_tag="VM713_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82839.1"
                     /translation="ALTAALRPIAAVAVPASVTAGQSVTLDGSASGAANGHTVSSYQW
                     SPVSGGLTLSITGGATAKASITAPACGVGTVSLTVTDDAGHSDTANVTVTPGSVTTSA
                     PAAAGQGACSSAAPAVLVEVCPAAGSVQAGGSATLTATLANTADAAVSWEVNGVAGGS
                     ATVGTITSAGVYTAPASVPMPATVTITAVSASDASAMGTSQLTITAPPSSGGGGGGSA
                     DWLTLLAGGTLLIARQRRAARR"
BASE COUNT          399 a          972 c         1024 g          408 t
ORIGIN      
        1 ccgactcggt accggcgatg gtccccgcgt cgatgtcagg gaccacggcg atcgcatagt
       61 ccagcggcac gatctccttg ggcagccgcc cgggggtggc gtcgaaggag aacggcgcgc
      121 cggccagcgc cggccatgaa gcgagcgcgg caagcagcgg cgcccagccg cgcacgagcg
      181 gaatgcgcat tggctggccc ctcaatagtt cgcggccgcg cgcactgcgc tcgcgggctt
      241 gtcgaggtcg gcgagcagct ccggcgcgac gatcgcaagc ggcgtcgtgc cgtaggaaag
      301 cagccggtcg tggaagtcgt ggagcgatgc gtcagcgccc ttgcgccgca ggtactcggc
      361 cagcaggttc tcgatctgca gcctccccac cgagtaggcg atgacatatc cggggccggt
      421 agcgattgcg gcgatcgccg cctcggcctg ctccctggtg aagccggtct cggcggcaaa
      481 gaactccacg gcgcgctcgt aagtccactc accgctcgcg agcttcggat ccacgaccgc
      541 gcgcgctccg cgcacccgtt cccagcgcgc gacagcgagc cgcccgtcga gatcgtcgcc
      601 gtagagcccg agccgcacga acatctcctc gccataaaag gcccagccct cggcgaacgc
      661 gctgctgtcc tcgacgcggc gcacgaagtc cgtatggcgc ttggcgatcg acagctgcat
      721 gaagtggccg ggcaacgcct cgtgcgccgc ggtggagaga atccggtcgc gatcgaagtc
      781 ctcgttcatg tcgagccggg ccgccgcctc ggccagcgac ttcggtgggg tgatgtagta
      841 gtagccggtg gtcgatagcg agaacagccg cggcggattc atcgaggcac cgggcgacac
      901 cggctgcagg aacgcgggcg tctccacgat ctgcatggta ccgagccagg cgggaatcgt
      961 gacgatgtcc tcatcggtaa cgaacttgcg cagctgcgcg atacagtcgc ggtagtaatc
     1021 gatgagggcc gcgccgcccg gcgcgagccc cccgccgctc ggggcgccga gcgcgacgcc
     1081 gcgctgctgc gccagctgcc gcagccaggc ctcctcggcc cagccatggg cgagctcatc
     1141 ctgcgcgata cgctcgacat cgtccgcaac gtagggcagc agctgctcct ccttcagcat
     1201 ccgatcgtag gccgctcgcc caatggcata gttttccggc caggacgcgg catgcgcctc
     1261 gaggtactgc cgggtctgcg cgatcgtgcc gagtgcggta tcgcgcgcgc gcaggaacgc
     1321 ggtgtactcc cgggagcgga cgccgagctg agtgcgtgcg gcctcggtga gcgcgccctt
     1381 gaggaaatcg ggcgcccccg cgagctgccg ggcaccgacg acgccataga ggtggccggg
     1441 cgtgttcgcg agccgttggc tggccgcgat ataggccggc gcgcccgcga ggcgccgggc
     1501 gatgtctgcc cagccgcgct tgaggtccgc cgcggtggcg ccctcctggc cgacgaccgg
     1561 caggtgctcg aactgcacga acaccacgtc gacgacgctg ttggcgccgc cggcgtagtc
     1621 cttgcggtcg gtctgataga ccagcagctc gttcaggctg cggctcagcg tggcacgcag
     1681 cagcgcggcg tcgtcgcggt ccacgagcgg ggcatgtgcg tcgaaggcgg ccgcgatcgc
     1741 ctccaggcgg cgttgccact cccgcagccg ctgtacctgc gcggcacgaa acgcctcgcc
     1801 cggcgagtgc agctcgccgt cgtggcctgg gatgccgagg taagtcgccg tcataggaaa
     1861 gacgtccgca gtcgtgtagg tgatgtcctg ggcaaggctc accagtgcct gatgctgggt
     1921 ctgtgcgagt gctgcgccgg cggcgagcgc ggtcgtcgcg gtcaacacga ggatccgagg
     1981 ggcgtgcatg aggctcctgc tcgagtcgcg ccgcggcggc gcgggccgcc gattatgttg
     2041 cggcgacgcg atggatgcca acgagaaggc ggccgggcgt tagcgacgtg cggcgcggcg
     2101 ctgccgggcg atgagcagtg tcccgccggc aaggagcgtg agccaatccg cgctgccccc
     2161 tcctccgccg ccgctgctcg gtggtgccgt gatcgtcagc tgcgaagtac ccatggcgct
     2221 ggcatcgctc gccgagacgg ccgtgatggt cacggtcgcg ggcataggca cgctcgcggg
     2281 agccgtgtag acccccgccg aggtgatcgt gccgacggtc gcgctgccgc cggcgacgcc
     2341 gttcacctcc cagctgaccg ccgcgtcggc cgtgttggcg agcgtggcgg tgagcgtcgc
     2401 gctgcctccc gcctgcacac tcccggctgc cgggcagacc tcgacgagga ccgcaggcgc
     2461 ggcgctcgag caggcgccct ggccggccgc agccggcgcg ctcgtggtca cagatcccgg
     2521 cgtgacggtg acgttggccg tgtcgctgtg gccggcgtcg tcggtgacgg tgagggagac
     2581 tgtgccgaca ccgcacgcgg gcgcggtgat cgaagccttc gccgtcgccc cgcccgtgat
     2641 gctcagcgtg agcccgccgc tgaccgggct ccactgatag ctgctgacag tgtggccgtt
     2701 ggccgcgccg ctggcactgc cgtcgagcgt cacgctctgg cccgccgtga cgctcgccgg
     2761 gaccgcgacg gcggcaatgg ggcgcagcgc cgcagtgagg gcg
//
LOCUS       DATLKL010000063         1547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_90948, whole genome shotgun sequence.
ACCESSION   DATLKL010000063 DATLKL010000000
VERSION     DATLKL010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90948"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="VM713_00850"
     CDS             <1..1503
                     /locus_tag="VM713_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVH82840.1"
                     /translation="SGGVRHRVRMSARPTAPARMLIVADDVRYAEWLRHHLDVLVPSA
                     SVSMLNLAEFERWCVTFSGRDCDLLMLACRFGSSPEDPNSPGLALLRRLRDQPAAPAV
                     IAIAEDGNELTAVRALQLGALDYLPKKLLTPERLSTAVRVALRRVERRVQKRLASLAQ
                     FEQDRLEQASKTSAESHGHVGDTVASAVPPGSDAPETAPTSTSEALQPPGVVALLTPP
                     ANEDFIPGYTLRVKVGESEKAAVYLATSAARGHNVALKVSKTLRDEAAGRQFLEREYT
                     AVQAIKSSLVVEIYDYGVHAGFEYLAMEYLPRGDLKARIQRGVTEREALHYLVRIAEA
                     LQVVHQAGLVHRDLKPPNVMLRENDTVALIDFGLARLIDGTTSTQTGLTAVLRGSPYY
                     MSPEQALGEQLDGRSDFYGLGIILYEMLTGQKPYTGGTALDVLQQHVSAPPPQLPESL
                     ERYQPLLTRLVAKARGERFETAAEIISAAGELLAGAAPEQRLSEPQPSAA"
BASE COUNT          251 a          575 c          504 g          217 t
ORIGIN      
        1 agcggcgggg tgcgtcatcg tgtgcgtatg agcgcccgac ccacggcacc ggcccgcatg
       61 ctgatcgtcg cagacgacgt ccgctacgcg gaatggctcc gtcaccacct cgacgtactg
      121 gtgccgagcg ccagcgtcag catgctcaac ctggcggaat tcgagcgctg gtgcgtgacc
      181 ttctcgggac gggactgcga cctgctgatg ctcgcctgcc gcttcggctc gagccctgaa
      241 gatcccaact cgccggggct cgcgctgctg cgccggctgc gcgaccagcc cgccgcgccc
      301 gccgtgatcg ccatcgccga ggatggcaac gagctcaccg cggtgcgcgc cctgcagctc
      361 ggtgccctgg attatctgcc gaagaagctc ctcaccccgg agcgcctgag caccgccgtg
      421 cgcgtggcgc tgcgccgcgt ggagcggcgc gtgcagaaac gcctggcaag cctcgcgcag
      481 ttcgaacagg accgactgga acaggcctcc aagaccagcg cggagagtca tggccatgtc
      541 ggcgacactg tggcaagcgc cgtgccgccg ggaagcgacg cgcccgaaac cgcgcccacc
      601 tccacatccg aggcgctgca gccgcccggc gtggtcgcgc tcctcacgcc acctgccaac
      661 gaggacttca ttccgggtta cacgctgcgc gtgaaggtcg gcgagtcgga aaaggccgcc
      721 gtctatctcg ccaccagcgc cgcgcgcggc cacaacgtcg cgctcaaggt ctccaagacg
      781 ctgcgcgacg aggccgccgg ccggcagttc ctcgagcgcg agtacaccgc ggtgcaggcc
      841 atcaagagct cgctggtcgt cgagatctat gactacgggg tgcacgccgg ctttgagtac
      901 ctggccatgg aatacctgcc gcgcggggac ctgaaggccc gcatccagcg cggcgtcacg
      961 gagcgcgagg cgctgcacta cctggtgcgc atcgccgaag cactccaagt cgtgcatcag
     1021 gcgggcctcg tgcaccgcga cctgaagccg ccgaacgtca tgctgcgcga gaacgacacg
     1081 gtggcgctca tcgactttgg cctggcgcgc ctgatcgacg gcaccaccag cacgcagacc
     1141 ggcctgaccg ccgtgctgcg cggctctccc tactacatga gtccggagca ggcgctcggc
     1201 gagcagctcg acgggcgctc cgacttctac ggcctcggca tcatcctcta cgagatgctg
     1261 accggacaga aaccctacac gggcggtacc gcgctcgatg tgctccagca gcacgtcagc
     1321 gcgccgcctc cgcagctgcc cgagtcgctc gagcgctatc agccactgct cacgcgcctc
     1381 gtggccaagg cgcgtggcga gcgcttcgag accgccgccg agatcatcag cgcagccggc
     1441 gagctcctcg ccggcgcagc tccagagcag cgcctctccg agccgcagcc cagcgcggct
     1501 taggcgcgaa ttgccgcgcg cgccgacggc tcgtgcgacg cttgccc
//
LOCUS       DATLKL010000064         2008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_68434, whole genome shotgun sequence.
ACCESSION   DATLKL010000064 DATLKL010000000
VERSION     DATLKL010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68434"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="VM713_00855"
     CDS             complement(<1..1170)
                     /locus_tag="VM713_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVH82841.1"
                     /translation="MKPPHDSAVAIAEFVFRGRRESVHGGCGRLPRALVAAVLTAAVA
                     AGCAVGPNYHRPSAPIPQRFKEAEGWKPAEPREVASGAEWWSVYGDTTLDSLEQQIDI
                     SNQTLKASEAAWRESLALVSAARAGFFPVIGVTATGTRSGGPGAGSANVGAGAAAAVQ
                     SHPKNQFELTGSASWTLDVWGKIRRTLESNIASARASEADLAAARLSAQAQLAIDYIE
                     LRVADEQQHLFDETVEAYKRSLEITQNQYKVGVVAKVDVITAETQLDGAESARIAIGV
                     TRATLEHAIAVLIGKAPGDFSLPPATLGTVVPVIPVGVPSALLERRPDIAAAERRMAA
                     ANAQIGIAVAAYFPDLTLSGSFGYASSIASGLVKAPNNLWAFGGSATDTLIDFGAR"
     gene            complement(1167..>2008)
                     /locus_tag="VM713_00860"
     CDS             complement(1167..>2008)
                     /locus_tag="VM713_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH82842.1"
                     /translation="GAVSTGSAVTVAAETAIPLAAFAHYAPGSTPLAVNHQGTFVATT
                     ISYNIAPNASISDASAAIKRAINEIGMPATIHGSSAGTARSAEQSFASEPVLILTALA
                     TVYIVLGILYESYIHPLTILSTIPSAGAGAVLALRLFGQEFGLIALIGLILLIGIVKK
                     NAIMMIDFALEAEREGRAPADAIYAASLLRFRPIMMTTMAAMLGALPLAFGTGDGAET
                     RQPLGISIVGGLVVSQILTLYTTPVMYLYLDRFGRWCSRQWARFHPETPPAAPAAPAQ
                     VRT"
BASE COUNT          327 a          655 c          707 g          319 t
ORIGIN      
        1 ccgtgctccg aaatcaatca gcgtatcggt tgcgctgccg ccgaacgccc agagattgtt
       61 gggtgccttg acgagaccgg aggcgatgct gctcgcgtag ccgaagctgc cgctcagggt
      121 gaggtcggga aagtaggcgg cgaccgcaat ccctatctgg gcattcgctg cggccatgcg
      181 gcgctcggcg gcggcgatat ccggccgccg ctcgagcagt gccgacggca cgccgaccgg
      241 gatcaccggc accacggtac cgagcgtcgc gggcggaagc gagaagtcgc ccggcgcctt
      301 gccgatgagt accgcgatcg catgctcgag ggtcgcgcgt gtgacgccga tggcgatccg
      361 cgctgactcg gcgccgtcga gctgcgtctc ggcggtgatc acgtcgacct tggcgacgac
      421 gcccaccttg tactgattct gagtgatctc gagtgagcgc ttgtaggcct cgacggtctc
      481 atcgaacaga tgctgctgct cgtccgcgac gcgcagctcg atgtaatcga tggcgagctg
      541 ggcctgcgcc gagagccgcg ccgcggcgag atccgcctcg ctggcgcgcg cgctggcgat
      601 gttgctctcg agcgtgcggc ggatttttcc ccagacgtcg agagtccagg acgcgctgcc
      661 ggtaagctcg aactgattct tcggatgcga ctgcaccgcc gcagccgcac cagctccgac
      721 gttggcagat ccggcccccg ggccgccgga gcgggtgccg gtcgccgtca cgccgatcac
      781 cgggaagaag ccggctcgtg ccgcgctcac gagcgccagc gactcgcgcc acgcggcctc
      841 ggaggctttg agcgtctggt tcgagatgtc gatctgttgc tcgagcgagt cgagcgtcgt
      901 atcgccgtac accgaccacc actcggcgcc gctcgctacc tcgcgcggct ccgcgggctt
      961 ccagccctcg gcctccttga agcgttgcgg gatcggagcc gacgggcggt gatagttcgg
     1021 gccgacggcg cagcccgcgg cgacggccgc ggtgagcaca gccgcgacca gcgcgcgcgg
     1081 caggcgcccg cagcctccat ggacggattc acggcggccg cggaagacaa attctgcgat
     1141 cgcgactgcg gagtcgtgcg gtggcttcat gtccgtacct gcgcaggcgc agccggtgcg
     1201 gcgggcggag tctccggatg aaaccgcgcc cactgccggc tgcaccagcg gccgaatcgg
     1261 tcgaggtaga gatacatgac gggtgtcgtg taaagcgtca ggatctggct caccaccagg
     1321 cctccgacga tcgagatacc gagcggctgg cgggtctccg cgccgtcgcc ggtgccgaag
     1381 gcgagcggca gcgctcccaa cattgccgcc atcgtggtca tcatgatcgg gcgaaaccgc
     1441 aggagcgagg cggcatagat cgcatccgcg ggcgcgcggc cttcgcgctc cgcctcgagc
     1501 gcgaagtcga tcatcatgat cgcgtttttc ttcacgatgc cgatgagaag gatcagaccg
     1561 atcagcgcga tgaggccgaa ctcctgcccg aacaggcgca gcgccagcac tgcgccggcg
     1621 cccgccgagg ggatggttga gaggatcgtg agcggatgga tatagctctc gtagaggata
     1681 ccgagcacga tgtagacagt cgcgagggcc gtgagtatca gcaccggctc actcgcgaat
     1741 gactgctcgg cggagcgtgc ggtccccgcg gagctgccgt gaatcgtggc cggcataccg
     1801 atctcgttga tagcacgctt gatcgccgcg gaggcatcgc tgatcgaggc attcggcgcg
     1861 atgttgtagg agatcgtcgt ggcgacgaaa gtcccctggt ggttgacggc gagcggcgtg
     1921 ctgcccggag catagtgcgc aaaggctgcg agcgggatcg cagtctcagc cgccaccgtc
     1981 acggccgagc cggtcgagac cgcgcccc
//
LOCUS       DATLKL010000065         1613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_79603, whole genome shotgun sequence.
ACCESSION   DATLKL010000065 DATLKL010000000
VERSION     DATLKL010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79603"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VM713_00865"
     CDS             <1..248
                     /locus_tag="VM713_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA pseudouridylate synthase B"
                     /protein_id="HVH82843.1"
                     /translation="DSGMQLTVARCEAAGGQGANRWYTVTVSGASGKQVRQLFERQGA
                     LVSRVLRTQLGPVALERTLGRGQARELTSDELRELLG"
     gene            complement(200..1000)
                     /locus_tag="VM713_00870"
     CDS             complement(200..1000)
                     /locus_tag="VM713_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009387635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HVH82844.1"
                     /translation="MTGTFDPRAHRATADELAGRVIGITGASDGLGRAVALACAQHGA
                     HVVLIGRSDQKLEAVHGAIAALGAPEAAIGILDLERALAADYDRLAAAVGERYGRLDG
                     LLHNAGLLGTLAPIEHYDVPTWCRVLHVNLTAAFALTQVLLPALKKSADASVVFTSSS
                     VGRRGRAYWGAYAVAKFALEGLAQVLADELQGTSAIRVNTLNPGRARTAMRRQAYPAE
                     DAATVSLPETLTGPYIALLGPASRGVTGQAFDAQPNSSRSSSDVSSRA"
     gene            complement(997..>1613)
                     /locus_tag="VM713_00875"
     CDS             complement(997..>1613)
                     /locus_tag="VM713_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HVH82845.1"
                     /translation="PLTRAVVGHLDGRDPAPLAGLVGLVDAAVWDLAAATFETRRELA
                     AYCERWAEAIIVPLARLAAPEVGPAARSLGAALREGELLGRLAPEARAGRLRLPLDEL
                     AQAAVDPARLGEPPWPPALASLLRTRHAQLRADLAAAVAALPRAAQTPLCALLVWAAL
                     ASAASRRAERRLPQALPGSDDAGLLDGWRAWRAARRARGGRFKL"
BASE COUNT          227 a          582 c          611 g          193 t
ORIGIN      
        1 tcgacagcgg catgcagctc acagtcgccc gctgcgaggc ggccggcggc cagggcgcca
       61 accgttggta caccgtgacc gtgagtggcg cgagcggtaa gcaggtacgc cagctcttcg
      121 agcggcaggg ggcgctggtg agtcgcgtgc tgcgcacgca gttgggtccg gtggcgctcg
      181 aacggacgct cggacgcggt caggctcgcg agcttacgtc ggatgagctc cgtgagctgt
      241 tgggttgagc gtcgaacgcc tgcccggtga ccccgcggct cgcaggcccg aggagcgcga
      301 tgtaggggcc ggtgagcgtt tccggcaacg acacggtcgc cgcatcctcg gccgggtagg
      361 cctggcggcg catcgcggtg cgggcgcgac ccgggttgag ggtgttcaca cggatggcgc
      421 tcgtaccctg cagctcgtcg gcgagcacct gcgcgagacc ctcgagcgcg aatttcgcga
      481 ccgcatacgc gccccagtag gcgcggccgc ggcgaccgac gctgctcgag gtgaagacca
      541 cggaagcgtc ggccgacttc ttcagcgccg gcagcagtac ctgggtgaga gcgaacgccg
      601 cggtcagatt cacgtgcagc acgcggcacc aggtcggcac gtcgtagtgc tcgatgggcg
      661 cgagcgtgcc gaggagacca gcgttgtgca gcagcccatc gagccgcccg tagcgctcgc
      721 cgacagccgc ggcgagccgg tcgtagtcgg cggcgagcgc gcgctcgagg tcgagtatgc
      781 cgatcgcggc ctcgggcgca ccaagagcgg cgatagcgcc atgcaccgcc tcgagcttct
      841 gatcgctgcg tccgatgagg accacgtgcg cgccgtgctg cgcgcaggcg agcgccaccg
      901 cgcgcccgag cccatcgctc gccccggtga tgccgatcac gcgccccgcg agctcatccg
      961 ccgtggcgcg atgcgcccgt ggatcgaatg tgccggtcat agcttgaagc gcccccctct
     1021 tgcccgacgc gcggcgcgcc aggcacgcca gccgtccagg agccccgcat cgtcgcttcc
     1081 cggtagcgct tgcggcaagc gccgctcggc gcggcgcgag gcggcgctcg cgagtgccgc
     1141 ccagacgagg agcgcgcaca gcggtgtctg cgcagcgcgc ggcagcgccg ccacggcggc
     1201 cgcgagatct gcgcgcagct gcgcgtgccg ggtgcgcagg agcgaagcca gcgccggagg
     1261 ccagggcggc tcaccgaggc gcgccgggtc caccgcggct tgcgcgagct catcgagcgg
     1321 cagccgcagc cgcccggcgc gtgcctcggg cgcgagccgc ccgagcagct ccccctcacg
     1381 cagcgccgca ccgagtgagc gcgcggcggg gccgacctcg ggcgccgcga gccgcgccag
     1441 aggaacgatg atcgcctcgg cccagcgctc gcagtaggca gccagctcgc ggcgcgtctc
     1501 gaaggtggcc gcggccaggt cccacaccgc cgcgtccacc aggccgacga ggcccgcgag
     1561 cggcgccggg tcgcggccat cgagatgacc cacgacggcg cgggtcagtg ggt
//
LOCUS       DATLKL010000066         2054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_158564, whole genome shotgun sequence.
ACCESSION   DATLKL010000066 DATLKL010000000
VERSION     DATLKL010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158564"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..676)
                     /locus_tag="VM713_00880"
     CDS             complement(29..676)
                     /locus_tag="VM713_00880"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HVH82846.1"
                     /translation="MDLALDRIAYVPSTGVRRVHEPPLVMATAASLAGYGLLVDEPKT
                     FPIEIVTWPAQGWRAIDANSGNQGGVTEGLFEFCWKGETLYARNHAVGDSYLFGWSNW
                     PEEAQSTSDRKRERALIWRANYHPDGGQLFYPLNGQSFVVPLALPGDNVRPESFVAFW
                     CDGRQALYLHPNVWHGAVVPHDDEAQFLDRQGRVHARVSVDFVKEFGCYLGVSLR"
     gene            complement(689..1237)
                     /locus_tag="VM713_00885"
     CDS             complement(689..1237)
                     /locus_tag="VM713_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVH82847.1"
                     /translation="MSVADEVVTLYSARGAAAYFGERVSMTEHGLQAAWFAQVEAAPE
                     PLIIAALLHDVGHLLQKVPDDLADWTSDAHHEEVGARWLAARFGPTISEPVRLHVPAK
                     RYLCATDAGYLRQLSSASVHTLALQGGPMSPDEARAFEREAHYRAAVRVRRWDDQGKV
                     AGLKTPPLEAYLQMLNAAARTR"
     gene            complement(1234..>2054)
                     /locus_tag="VM713_00890"
     CDS             complement(1234..>2054)
                     /locus_tag="VM713_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVH82848.1"
                     /translation="RLWLEGARLEAARDFAWVELAAARAEPRVRLEWLARLAQDGIAF
                     LRVVPCQAAAILDAVALVGRLAETNYGRVFDVRSVPQPENLAYSDLGLGLHTDNPYRE
                     PVPGFQALHALVTSPEGGDSLFADGFALAAYFKDTAADAFGLLTTTAVPFHYRSADAE
                     LYAERPLIQLSCTGELAAVHYNNRSIRVAPRTGSAFYAAYRSFAALLREPRFQLRLKL
                     ADGDLVVFDNQRVLHGRTAFTSAKHPRHLQGCYLTRDSVLSEIGLLRRSLGGPA"
BASE COUNT          340 a          692 c          720 g          302 t
ORIGIN      
        1 cgcctgagct tgcgctgtgc gcgccgcgct aacgcagcga gacgccgagg tagcagccga
       61 actccttgac gaagtcgacc gagacgcggg cgtgtacgcg cccctggcgg tcgaggaact
      121 gcgcctcatc gtcatgtggc acgaccgcgc cgtgccagac gttaggatgc agataaagcg
      181 cttggcggcc gtcgcaccag aaggcgacga aagattccgg cctcacatta tcgcccggca
      241 gcgcgagcgg cacgacgaag ctctggccat tgagcggata gaagagctga ccaccgtcgg
      301 ggtgatagtt ggcgcgccag atgagggcgc gctcgcgctt gcggtcgctc gtgctctgcg
      361 cctcctcggg ccagttgctc cagccgaaga gataactgtc gcccaccgcg tggttgcggg
      421 cgtagagcgt ctcgcccttc cagcagaact cgaacaatcc ctcggtcacc ccgccctgat
      481 tgccgctgtt ggcgtcgatc gcgcgccagc cctgcgcggg ccacgtgacg atctcgatcg
      541 ggaaagtctt cggctcatcg acgagcaacc cgtagcccgc aaggctcgca gcggtcgcca
      601 tgaccagcgg cggctcgtgc acgcggcgca cgcccgtcga gggtacgtag gcgatccggt
      661 cgagtgccag gtccatagcc gtttgtcgtc agcgcgtgcg ggctgccgcg ttcagcatct
      721 ggagataggc ctcgaggggc ggggtcttga gacccgcgac cttgccctgg tcgtcccagc
      781 gccgcacgcg caccgccgcg cggtaatggg cttcgcgctc gaacgcgcgc gcttcatcgg
      841 gcgacatcgg gccgccctgg agggcgagcg tatggaccga ggcggagctc aactggcgca
      901 agtacccggc atcagtcgcg cagagatagc gcttcgccgg tacgtgcagg cgcaccggct
      961 cactgatcgt ggggccgaag cgtgcggcaa gccagcgggc gccgacttct tcgtggtggg
     1021 cgtcggaggt ccagtcggcg aggtcgtccg gtaccttctg cagcagatgg ccaacgtcat
     1081 gcaggagagc ggcgatgatg agcggctcgg gagccgcctc gacctgggcg aaccacgccg
     1141 cctgcagtcc atgctcggtc atcgacacgc gctcgccgaa gtacgcagcc gcgccgcgcg
     1201 cgctgtagag cgtgaccact tcgtccgcga cgctcacgcc ggtcctccca ggctgcgccg
     1261 caggaggccg atctcgctca gcacgctgtc gcgcgtcagg tagcagccct ggaggtgccg
     1321 cggatgcttc gccgaggtga atgcagtgcg gccatgcagc acccgctggt tgtcgaacac
     1381 gaccagatcg ccgtccgcga gctttagccg caactgaaag cgcggctcgc gcagcagcgc
     1441 ggcgaagctc cggtacgcgg catagaacgc tgaccccgtg cgcggtgcga cgcggatcga
     1501 gcggttgttg taatggacgg ccgccaactc gcctgtgcac gagagctgga tcagcggccg
     1561 ctcggcatag agctccgcgt ccgccgagcg gtagtgaaag gggactgccg tggtggtgag
     1621 gagcccgaag gcatcggctg cggtgtcctt gaagtacgcc gccagcgcaa agccgtcggc
     1681 gaacaggctg tccccgccct cgggcgaagt cacgagcgcg tgcagcgcct gaaagcccgg
     1741 caccggctcg cggtagggat tatcggtgtg caggccgagt cctaagtcgg aatacgcgag
     1801 gttctccggc tgcggcaccg agcgcacatc gaacacccgc ccgtagttgg tctcggcgag
     1861 gcgcccgacg agcgctaccg catcgaggat cgcggcggcc tggcagggca cgactcgcag
     1921 gaaggcaatg ccatcctgcg cgaggcgcgc gagccactcg aggcgcacgc gcggctcggc
     1981 gcgcgccgcg gcgagctcca cccaggcgaa atcgcgcgcg gcttcgagcc gcgcaccctc
     2041 gagccacagc cggt
//
LOCUS       DATLKL010000067         2109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_102269, whole genome shotgun sequence.
ACCESSION   DATLKL010000067 DATLKL010000000
VERSION     DATLKL010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102269"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VM713_00895"
     CDS             <1..789
                     /locus_tag="VM713_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HVH82849.1"
                     /translation="VWNPSTAFLVPGTKVPAAFIFATEDGTLSAWAGGLNPPSDAVLA
                     FDNSANPTAASGAVYKSLVFGLRAKGLLLYAANFRHATVDVFGPTGADGMFTPATTGG
                     GFVDPGIPAGYAPFGIANIDGDLFVTYAQQNDAKHDDVAGVGHGFVDVFDTDGHLLRR
                     FASRGPLNSPWAVTRASFAFGRFSGRILIGNFGNGHINAFDNDGRFIGELEDGHGRPV
                     VIDGLWTLTLGGGRGSSSEALYFAAGPNGETQGLFGTIAPVTAP"
     gene            901..1530
                     /gene="lepB"
                     /locus_tag="VM713_00900"
                     /pseudo
     CDS             901..1530
                     /gene="lepB"
                     /locus_tag="VM713_00900"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
     gene            complement(1561..>2109)
                     /locus_tag="VM713_00905"
     CDS             complement(1561..>2109)
                     /locus_tag="VM713_00905"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HVH82850.1"
                     /translation="RGINVVGRRKLLMKDLAAIFEHGGLRSVRTYIQSGNVVFQSARG
                     SARTLAPLLAELIVKKSGFQPHVIVLSVPELAAAIRGNPFPQAHRDHKSLHLFFLSGR
                     PARPDLESLARLKAGREAFELRGAVFYLYTPHGFPQCALHDKVERFLGVQATARNWRT
                     ANQLLLMARAPVLSAKRHQKAV"
BASE COUNT          339 a          696 c          704 g          370 t
ORIGIN      
        1 gtatggaatc cttcgaccgc gttcctggtg ccaggcacca aggtgcccgc cgcgttcatt
       61 ttcgcgaccg aggacggcac cctctccgca tgggccggcg gattgaatcc gcctagcgac
      121 gcggtcctcg cgttcgacaa ctcggcgaat cccactgcgg cgagtggggc cgtttacaag
      181 agcctggtgt ttgggctccg cgcgaagggc ctgttgctgt acgcggcgaa cttccgccat
      241 gccacggtcg atgtcttcgg acctactggg gcggacggaa tgtttacgcc ggcgaccacc
      301 ggcggcggtt tcgtcgaccc cggcatcccc gctggctacg cgccgtttgg catcgctaac
      361 atcgacggcg acctgttcgt cacctacgct cagcagaacg acgcgaagca cgacgacgtg
      421 gcgggcgtgg gtcacggatt tgtggatgtg tttgacaccg acggccatct gctgcgccgc
      481 ttcgccagcc gtggcccgct caactctccc tgggcggtga cccgcgcatc cttcgcattc
      541 gggcgcttca gcggcaggat cctgatcggc aatttcggca acgggcacat caacgccttc
      601 gacaacgatg ggcggttcat cggtgagctc gaggacggtc atggaaggcc ggtggtgatc
      661 gatggtctgt ggacgctgac cctcggcggc ggtcgcggct cgagctcgga agccctgtac
      721 ttcgcggcgg ggccgaatgg cgagacgcag ggactcttcg ggacgattgc tccggtaact
      781 gccccttgag gcgagccccg ggcggtggcg cgcccaccgc ccgggagtgc ggccggaaca
      841 cacaccacgc ccggcggtcc aagctgtcgg aagtacgcga acccggcacg ccgtgagacc
      901 atgaccttcg aaaaactccg cacctggtgg cgcaactacc ggagcaccct ggtctttctc
      961 gcgctgatgc tgggctttcg ctcggcctgg gccgattggg tgacggtgcc caccggatcg
     1021 atgaatccga cgatcctcga aggcgaccgt ttgctcgtcg acaagcacgc gttcggcctg
     1081 cgggtcccct tcacccgggt acacctcacc gccggcgagg atcccgcgcg cggggatatc
     1141 gtcgtgttcg attcacccgt cgatggcacc tcgctggtga agcgggtggt cgggcttccg
     1201 ggcgatgtgg tggcgctcga ggcggatcgg ctcaccgtga acgggcgccc agcgagctat
     1261 tcgatcgaga acatcgcgag cgtccacgga ctgctcgccg cgaccgcggc ccaacatccg
     1321 ctgctgctgc gcgagaccgg gctcggcaag gctcacgagg tgatgatcct ccccgggcac
     1381 ggggcgcgcc gctctctcga gggcgtcgtg gtgccggaag gcatgtactt catgatgggg
     1441 gacaaccgcg acaacagcgc agattcgcgc tatttcggtt tcgtgccccg ccgcgacatc
     1501 atcgggcgcg ccacgcgcgt gctggtctcg ctgaatccgg accgctacta cgcgccgcgc
     1561 tcagaccgct ttctgatgcc gcttggctga gagcactgga gcgcgggcca tcagcagcag
     1621 ctgattggcc gtgcgccagt tgcgcgcggt ggcctgtact ccgaggaagc gctcgacttt
     1681 gtcgtgcagg gcgcattgcg gaaaaccgtg gggcgtgtag aggtaaaaga ccgctcccct
     1741 gagctcgaac gcttcgcgcc cggctttgag ccgcgcgagt gactcgagat cgggccgcgc
     1801 cgggcgcccc gacaggaaga agagatgcag cgacttgtga tcgcgatgcg cctgcggaaa
     1861 cgggttgccg cggatcgccg ccgcgagctc cggcaccgac agcacgatca cgtgcggttg
     1921 gaaccctgac ttcttcacta tcagctcagc gagcagcggc gccagggtcc gcgcgctccc
     1981 ccgtgcactc tgaaatacga cattgccgct ctgaatgtag gtgcgcacgg agcgcaatcc
     2041 gccgtgctcg aagatcgcag ccagatcctt catcagcagc ttccggcggc cgacgacgtt
     2101 gatgccgcg
//
LOCUS       DATLKL010000068         1517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_136069, whole genome shotgun sequence.
ACCESSION   DATLKL010000068 DATLKL010000000
VERSION     DATLKL010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136069"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VM713_00910"
     CDS             <1..309
                     /locus_tag="VM713_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVH82851.1"
                     /translation="RAEGIVSGGHLVAKALKNEGVDTIFTLCGGHIIDIYDGCLDEGI
                     KIIDVRHEQVAAHAADGYARMAGRPGCAVVTAGPGTTDAVTGVANAFRAESPFLLIGG
                     "
     gene            331..>1517
                     /locus_tag="VM713_00915"
     CDS             331..>1517
                     /locus_tag="VM713_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVH82852.1"
                     /translation="MGSLQDLPHVDIMQPITKFAATVPSTARIADMVSMAFRECMNGA
                     PGPSFLEIPRDVLDAEVELAKARIPAAGHYRASTRLVADPDDIEKLADILVKSERPCI
                     LLGTQVWTCRAAAAAAEFVRKLNIPAYMNGAGRGTLPPGDPHHFHRTRALAFEKADVI
                     VIVGTPFDFRMGYGRRLRADATVAQVDMDYRTVGKNRDISLGLVGDVGMILAAVTAAA
                     TGRADNGAKRRRAWLEELGKAEAAGEQKLMAQFRSEATPINPYRVAWEINEFLSEDTI
                     YIGDGGDVVTITAQAVQPRRPGQWMDPGPLGTLGVGVPFAMAAKFVHPDKEVLCYFGD
                     GAFAMTGFDLETCIRFKLPFLGVIGDNSAMNQIRYGQIAKYGPQRGDVGNKLGNVPFS
                     KFA"
BASE COUNT          257 a          530 c          498 g          232 t
ORIGIN      
        1 cgcgcggaag gcatcgtttc gggtggtcac ctggtggcca aggctctgaa gaacgagggc
       61 gtcgacacga tcttcacgct ctgcggcggc cacatcatcg acatctatga cggctgtctc
      121 gacgagggca tcaagatcat cgacgtgcgc cacgagcagg tcgcggcgca tgccgccgac
      181 ggctatgcgc gcatggccgg ccggcccggc tgcgctgtgg tcacggccgg ccctgggacg
      241 accgacgcgg tgacaggggt cgcgaacgcg ttccgcgccg aaagcccgtt cctcttgatc
      301 ggcggctagg tcgcgctctc ccggcacaag atgggctcgc tccaggacct gccccatgtc
      361 gacatcatgc agccgatcac caaatttgcg gccacggtgc cgagcacggc gcggatcgcc
      421 gacatggtga gcatggcctt ccgcgagtgc atgaacggcg cgcccggccc gtccttcctc
      481 gagatcccgc gcgatgtcct cgacgccgag gtcgagctcg ccaaggcgcg catccccgcc
      541 gcaggccact accgcgcctc caccaggctg gtcgccgacc ccgacgacat cgagaagctc
      601 gcggacatcc tggtgaaatc ggagcggccc tgcatcctgc tgggcacgca ggtctggacc
      661 tgccgcgccg ccgctgccgc cgccgagttc gtacgaaagc tcaacatccc ggcctatatg
      721 aacggtgccg ggcgcggcac gctgccgccg ggcgacccgc atcactttca ccgcacgcgc
      781 gcactcgcct tcgagaaagc tgacgtgatc gtgatcgttg gcacaccatt cgacttccgc
      841 atgggctacg gccggcgtct tcgcgccgac gcgacggtcg cgcaggtcga tatggactac
      901 cgcaccgtcg gcaagaaccg cgacatctcg ctcgggctcg ttggcgacgt gggcatgatc
      961 cttgccgccg tcaccgcggc ggcgaccggg cgggcggaca acggagccaa gcgccgccgg
     1021 gcctggctcg aggagctggg caaggccgag gccgcgggag agcagaagct catggcacag
     1081 ttccgctccg aggcgacccc gatcaatcca taccgggtcg cttgggagat caacgagttc
     1141 ctgagcgagg acacgatcta catcggcgac ggcggcgacg tggtgaccat caccgcccag
     1201 gcggtacagc cgcgccggcc cggccagtgg atggatccgg ggccgctcgg cacgctcggc
     1261 gtcggcgtgc ccttcgcgat ggcggccaag ttcgtgcatc cggataagga ggtgctctgc
     1321 tacttcggcg acggcgcctt cgccatgacc ggattcgacc tcgagacctg catccgcttc
     1381 aagctgccct tcctcggcgt gatcggggac aactcggcta tgaaccagat tcgctacggc
     1441 cagatcgcca agtacggccc acagcgcggc gacgtgggca acaagctcgg caacgtgcca
     1501 ttctcgaagt ttgccga
//
LOCUS       DATLKL010000069         2808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_181208, whole genome shotgun sequence.
ACCESSION   DATLKL010000069 DATLKL010000000
VERSION     DATLKL010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181208"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..733
                     /gene="yjgA"
                     /locus_tag="VM713_00920"
     CDS             230..733
                     /gene="yjgA"
                     /locus_tag="VM713_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HVH82853.1"
                     /translation="MSDDDEGERPSKSARKRAAHAAQDLGEALIGLPDEELAALPIPE
                     ELAAAVRAARRITSRAGGARQRQYIGKLMRALDPEPIRAALAARGAVSAREAERFRRV
                     EEWRTRLIEGGAVALAELKRAHPGIDTSEWAARVAAAQAERSRRTGAGAARELFRALR
                     ALFDTMP"
     gene            733..1233
                     /gene="purE"
                     /locus_tag="VM713_00925"
     CDS             733..1233
                     /gene="purE"
                     /locus_tag="VM713_00925"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HVH82854.1"
                     /translation="MKALVGIIMGSASDWETMQAAAEILDRLGIAYEVRVVSAHRTPD
                     LLFEYASGARTRGLEVLIAGAGGAAHLPGMAAAKTTLPVLGVPVQSKTLGGLDSLLAI
                     VQMPAGVPVATFAIGSAGAANAALCAAAILANRYPAVQTALEAFREKQTAAVLANSDP
                     RTHPRT"
     gene            1230..2339
                     /locus_tag="VM713_00930"
     CDS             1230..2339
                     /locus_tag="VM713_00930"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HVH82855.1"
                     /translation="MTIGIVGAGQLGRMLALAGYPLGLDFLCLDPASDAPAGQVAPLL
                     HGAFTDPALLAELARRCEIVTFDWENVPVASLRAMQRGAHRARIAPPAAALACGQDRV
                     SEKRLFERLRIPTTRWRAVDSRSELDAAIAAVGLPGVLKTRRLGYDGKGQALLRTRAD
                     AGRAWERLGSAPLLFEEWVPFECEVSVIGARGARAEMAIYPLCGNVHGAGILRLTCAP
                     YGPRGWHEQARRHLRRLLAHFGYRGILTLEFFVRAGKLIANEMAPRVHNSGHWTIEGA
                     ATSQFENHLRAILGLPLGSTRALGYSAMINLIGRIPPRARLLALEGVHLHDYGKQPRP
                     GRKVGHCTVVEPTVSRRDARARRLAAELAAGVRIP"
     gene            2363..>2808
                     /locus_tag="VM713_00935"
     CDS             2363..>2808
                     /locus_tag="VM713_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH82856.1"
                     /translation="MYLEPFKLKELPFRLSPDPQFLYLSKQHARAKAYMESTIWFTDG
                     FVVITGDIGSGKTTLIESFLKEVPADVVVAQINQTQVSATDFLQAVLVQFGFSPFKMR
                     KAELLSTLNNFLIEQHAAGRKVLLIVDEAQNLEARVLEEIRLLSGV"
BASE COUNT          397 a          998 c          993 g          420 t
ORIGIN      
        1 cgctggtact cgacggtgtc gacttcgccg aggcgcacgc tcaccgagag cccgcggctc
       61 accgaagcgt ctgcttcgca ctgcgaggcg ccctggcggc gcgcctcctc gagcgtagag
      121 ctgacgaggt cggcgagctg cgcagagccg gcaagctcgg gggtcgatgg catggtgcta
      181 aattgtagca gcgggccggt accctcaggt ccgcgaagga agcaacgact tgagcgacga
      241 cgatgagggc gagcgcccca gcaagagcgc gcgcaagcgc gctgcgcatg cggcccagga
      301 tctgggcgag gcactgatcg gcctgccgga cgaggagctc gccgcgcttc ccattcccga
      361 ggagctcgcc gccgcggtgc gcgccgcccg ccgcatcacc agccgcgcgg gcggcgcccg
      421 ccagcgccag tacatcggca agctgatgcg cgcgctcgat cccgagccga tccgggccgc
      481 gctcgcggcg cgcggcgcgg tgagtgcgcg cgaggccgag cgctttcgcc gcgtggagga
      541 gtggcgcacg cggctgatcg agggcggggc ggtggcgctc gcggagctca agcgcgcgca
      601 ccccggcatc gacacctccg agtgggcggc ccgcgtggcg gcggcgcagg ccgagcgcag
      661 ccgccgcacg ggcgcgggcg cggcgcgcga gttgtttcgc gctctgcgcg cattgttcga
      721 tacaatgccg tgatgaaggc cctcgtcggc atcatcatgg gctccgcttc cgactgggag
      781 accatgcagg ccgccgccga gatcctcgat cgcctcggca tcgcttacga ggtgcgcgtg
      841 gtgtccgcgc accgtactcc ggacctactg ttcgaatacg cctccggcgc gcgcacgcgc
      901 ggcctcgagg tgctgatcgc cggtgcgggc ggcgccgctc acctgcccgg tatggccgcg
      961 gcgaagacca ccctgccggt gctcggcgtg ccggtgcagt cgaagaccct gggcgggctc
     1021 gattcactgc tggcgatcgt gcagatgccc gccggcgtcc ccgtggccac cttcgccatc
     1081 ggcagcgccg gagctgccaa cgcggcgctg tgcgccgcgg cgatcctcgc caaccgctac
     1141 cccgccgtac agacggcgct cgaggcattc cgcgagaaac agaccgcggc cgtgctcgcc
     1201 aattccgatc cgcgcacgca cccgcgcaca tgaccatcgg catcgtgggc gccggccagc
     1261 tcggccgcat gctcgctttg gcgggctatc cgctcggtct cgatttcctg tgcctggacc
     1321 cggcgagcga cgcgccggcg ggccaggtgg cgccgctcct gcacggcgcc ttcaccgacc
     1381 ccgcgctgct cgcagagctc gcgcggcgct gcgagatcgt cacttttgac tgggagaacg
     1441 tgccggtggc gagcctgcgc gccatgcagc gcggcgcgca ccgcgcgcgc atcgccccgc
     1501 cggcggcggc gctcgcctgc gggcaggacc gtgtcagcga gaagcgcctg ttcgagcgac
     1561 tccggattcc caccacccgc tggcgtgcgg tcgattcgcg ctcggagctc gacgcggcca
     1621 tcgccgcggt ggggctgccg ggcgtgctca agacgcgacg cctcggctac gacggcaagg
     1681 gtcaggcgct gctgcgcacg cgcgccgatg ccggtcgcgc ctgggagcgc ttggggtcgg
     1741 cgccgctcct tttcgaggag tgggtgccgt tcgagtgcga ggtgtcggtg atcggcgcgc
     1801 gcggcgcgcg cgctgagatg gcgatctatc ccctgtgcgg caacgtgcac ggggcgggaa
     1861 tcctgcgcct gacctgcgcg ccgtacgggc cgcggggctg gcacgagcag gcgcggcggc
     1921 acctgcggcg cttgctggcg catttcggct accgcggcat cctcaccctc gagttcttcg
     1981 tacgcgcggg caagctgatc gccaacgaga tggctccgcg ggtgcacaac tccgggcact
     2041 ggaccatcga gggtgccgcg accagccagt tcgagaatca tctgcgcgcg atcctcgggc
     2101 tcccgctcgg ctcgacgcgc gcgctcggct acagcgccat gatcaatctg atcgggcgca
     2161 tcccgccgcg cgcgcggctc ctggccctcg agggcgtgca cctgcatgac tacggcaagc
     2221 agccgcgccc gggacgcaag gtcgggcact gcaccgtggt cgagccgacc gtcagccgcc
     2281 gggacgcgcg tgcgcggcgt ctggccgctg aattggccgc cggggtgcgc atcccgtaac
     2341 atccttgggg agtgcgactc ccatgtacct cgagccgttc aaactcaagg agctgccgtt
     2401 ccggctgagt ccggacccgc aattccttta cctgtcgaaa caacacgccc gtgccaaggc
     2461 gtacatggag tcgacgatct ggttcaccga cggcttcgtg gtgatcaccg gcgatatcgg
     2521 ctcggggaag accaccctca tcgagtcgtt tctcaaggaa gtcccggccg acgtcgtggt
     2581 cgcgcagatc aaccagacgc aggtctcagc cacggatttc ctacaggcgg tgctggtgca
     2641 gttcgggttc tcgcccttca agatgcgcaa ggcggagctg ctcagcaccc tcaacaactt
     2701 cctcatcgag cagcatgccg ccggacgcaa ggtgctgctc atcgtcgacg aggcacagaa
     2761 tctcgaggcg cgcgtgctcg aggagatccg cctgctgtcg ggcgtcga
//
LOCUS       DATLKL010000070         1571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_34689, whole genome shotgun sequence.
ACCESSION   DATLKL010000070 DATLKL010000000
VERSION     DATLKL010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34689"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..432
                     /locus_tag="VM713_00940"
     CDS             109..432
                     /locus_tag="VM713_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006792396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HVH82857.1"
                     /translation="MRGTFNDMMKQVQAMQANMQKAQAEIASLEVIGEAGGGMVKITM
                     NGRHEVKRVQIEAAVAGEDREMLEDLIAAAANDAVHKVEALVQQKMSHLTAGLALPPG
                     MKLPF"
     gene            434..1030
                     /gene="recR"
                     /locus_tag="VM713_00945"
     CDS             434..1030
                     /gene="recR"
                     /locus_tag="VM713_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003254304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HVH82858.1"
                     /translation="MKHSPHLTQLIEALRTLPGVGPKTAQRMAFHLLQEGRPGARALA
                     GALDAALESVVRCRRCRMLTETELCSICSAAQREAALLCAVESPADVVAIESSGSYRG
                     RYFVLMGHLSPLDGIGPEQLGVRELEAILDEGSVRELILATNSTVEGEATAHFLSELA
                     VRRGIRASRIAYGVPVGGELEYVDGGTLAHALAGRQSV"
     gene            complement(1034..>1571)
                     /gene="rsgA"
                     /locus_tag="VM713_00950"
     CDS             complement(1034..>1571)
                     /gene="rsgA"
                     /locus_tag="VM713_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HVH82859.1"
                     /translation="GLAAELAAYAVAGYGAVRCSAHTGSGTDALLAALPPGAAAALVG
                     QSGVGKSSLIARLLPEERLAIGELARADGGRHTTTAARLFDLPERRALIDSPGVRDFV
                     PAVDSLEPRSLGFTEVERLSAGCRFLDCRHMREPGCAVRAALDAGTFHARRYESYRRL
                     RRLREDLSAARGPGRSRR"
BASE COUNT          244 a          540 c          566 g          221 t
ORIGIN      
        1 cagggcctgc gcgagcgctt cggcgcgacc gtgctgcccg agaccgtacg gccggtaaaa
       61 tgatcacctt ccatcgtgtg gcgcacggcg cctgacggac cctccaccat gcgcggcact
      121 ttcaacgaca tgatgaaaca agtgcaggcg atgcaggcca acatgcagaa ggcccaggcc
      181 gagatcgcca gcctcgaggt gatcggcgaa gccggcggcg gcatggtgaa gatcaccatg
      241 aacggccggc atgaggtgaa gcgggtgcag atcgaggccg cagtcgcggg cgaggaccgc
      301 gagatgctcg aggatctgat cgcggccgcg gccaacgacg ccgtccacaa ggttgaggcg
      361 ctcgtgcagc agaagatgag ccatctcacc gcggggctgg ctctgccgcc gggaatgaag
      421 ctgccgttct agcgtgaagc actcgccgca tctcacacag ttgatcgaag cgctccgtac
      481 cctgcccggg gtggggccga agaccgccca gcgcatggcg tttcacctgc tgcaggaggg
      541 gcgcccaggt gcgcgcgcgc tcgcaggcgc gctcgatgcg gcgctcgagt ccgtggtgcg
      601 ctgccggcgc tgccgcatgc tcaccgaaac ggagctttgc tcgatctgct ccgccgcgca
      661 gcgtgaggcg gcgctgctgt gcgcggtcga gtccccggcc gatgtcgtgg cgatcgagtc
      721 ctcgggcagc taccgcgggc gttacttcgt gctgatggga cacctttcac ccctcgacgg
      781 tatcggcccc gagcagcttg gagttcgcga gctcgaagca atcctcgacg agggcagtgt
      841 gcgtgagctc atcctcgcca ccaactcgac cgtcgagggc gaggcgaccg cgcatttcct
      901 gagcgagctc gcggtgcgtc gcggcattcg cgcgagccgc atcgcctacg gtgtgccggt
      961 cgggggtgag cttgagtacg tcgacggcgg cacgctcgcg cacgccctcg cgggccggca
     1021 gtcggtctga cgctcagcgc ctgctgcgcc cgggaccccg ggcggcgctc aagtcctcgc
     1081 gcagccttct caggcgccgg tagctttcat agcgccgcgc atggaacgtg ccagcatcaa
     1141 gcgccgcgcg caccgcgcag ccgggctcgc gcatgtggcg gcagtcgagg aagcggcacc
     1201 ccgcagacaa gcgctcgacc tcggtgaagc cgaggctgcg gggctccagg ctgtcgacgg
     1261 cggggacgaa gtcgcgcact ccgggagagt cgatcagcgc gcgccgctcc ggcagatcga
     1321 acagccgcgc cgcggtcgtg gtgtgccgtc ctccgtcggc acgcgccagc tcgccgatcg
     1381 cgaggcgctc ctcgggcagc agccgcgcga tgagcgaaga cttgccgacg ccggactgcc
     1441 cgacgagggc ggccgcggcg ccgggcggca gtgccgcgag cagcgcgtcc gttccgctgc
     1501 cggtgtgcgc cgaacagcgt acggcgccgt agccggccac agcgtaggct gcgagctcgg
     1561 cggcgagccc c
//
LOCUS       DATLKL010000071         2113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_147363, whole genome shotgun sequence.
ACCESSION   DATLKL010000071 DATLKL010000000
VERSION     DATLKL010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147363"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VM713_00955"
     CDS             complement(<1..313)
                     /locus_tag="VM713_00955"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82860.1"
                     /translation="MLKLYGTELKSHLLLGTARFPSPAVLTAAITAAGAEVVTVSLRR
                     EAAGERAGQGFWSIIRSLNVRVLPNTAGCHTVKEAVTTAHMAREVFGTPWVKLEVIGE
                     DD"
     gene            complement(480..1490)
                     /locus_tag="VM713_00960"
     CDS             complement(480..1490)
                     /locus_tag="VM713_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH82861.1"
                     /translation="MRVTVRGAGVAGLACAVELAERGATVEVLEQAAALAGAGCSWFA
                     GGMLAPWCELESGGPLIARLGEEGIAWWRRLFPATAQRGTLVIAHGRDAAELVQFARR
                     TERYRTIGAEALAALEPDLAGRFGTALHFEDEAHLDPRAALGALLVRARELGVSMRFA
                     VDAAAVAPAGRELLDCTGLAARRALTELRGVRGEMLLVRAPDVSLTRPVRVLHPRIPV
                     YVVPRADHVYMIGATVIESDGGQRISARSLLELLSAAYALHPGFGEAEILETGVGLRP
                     AFPDNLPRLRRVGAALYVNGLYRHGFLLAPALAARAARALLEGQHFPEVMDEDPRQRR
                     LA"
     gene            complement(1495..>2113)
                     /locus_tag="VM713_00965"
     CDS             complement(1495..>2113)
                     /locus_tag="VM713_00965"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HVH82862.1"
                     /translation="WARDCQVMIEGPGHVPMHKIKENMDKQLAACGEAPFYTLGPLTT
                     DIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDRNDVKTGVITYKIAAHAAD
                     LAKGHPAARARDDALSRARFDFRWQDQFNLSLDPDTACAFHDETLPKEGHKVAHFCSM
                     CGPKFCSMRISHEVRAEARAQGMREMAEKFRAGGSELYVPAAKEG"
BASE COUNT          316 a          764 c          740 g          293 t
ORIGIN      
        1 tatcgtcctc gccgatgacc tcgagcttca cccacggcgt gccgaacacc tcgcgtgcca
       61 tgtgggccgt cgtgaccgct tccttcactg tgtggcagcc ggcggtattc ggcagcacgc
      121 gcacgttcag ggagcggatg atcgaccaga agccctggcc ggcccgctcc ccggccgctt
      181 cgcgccgcag cgacacggta accacctcgg cgccggccgc ggtgatggca gccgtcagga
      241 ccgccggcga gggaaagcgc gcggtgccga gcagcagatg cgacttgagc tcggttccgt
      301 agagcttgag cattcaccct ccctgcatcg gcgcgagcac ttcgacgcga tcgccgtcgg
      361 ccagcggggt gcgcagccgc gccggcgcgg cgacgaactc gccgttgacc gcggtcgaga
      421 cgatgcggcc gccgtagccg agctcctcga gcgcggccgc gagctcgcgg ccttcggtct
      481 cacgccagac gccgttgacg aggatcctcg tccatcacct ccgggaaatg ctgcccctcg
      541 aggagcgcgc gcgccgcgcg cgcagcgagc gccggcgcga gcagaaagcc atgccgatag
      601 aggccattca cgtagagcgc agcgcccacg cgccgcaggc gcggcagatt gtcgggaaag
      661 gcggggcgca gcccgacgcc ggtctcgagg atctccgcct cgccgaaccc cggatgcagc
      721 gcgtacgctg cggagagcag ctcgaggagg gagcgtgcgc tgatgcgctg ccccccgtcg
      781 ctctcgatca cggtggcgcc gatcatgtac acgtgatcgg cgcgcggcac cacgtacacc
      841 ggtatgcgcg ggtgcagtac ccgcaccggg cgggtcagcg acacgtccgg cgcccgtacc
      901 agcagcatct cgccccggac gccgcgcaac tccgtgaggg cgcggcgcgc cgcgagcccg
      961 gtgcagtcga gcagctcgcg cccggcgggc gctacggcag cggcgtcgac cgcgaagcgc
     1021 atgctgaccc cgagctcgcg cgcgcgtacg agcagcgcgc cgagcgcggc gcgcgggtcg
     1081 agatgagcct cgtcctcaaa gtggagcgcg gtgccgaagc ggcccgccag gtccggctcg
     1141 agggccgcca gggcctcagc gccgattgtc cggtagcgct cggtgcgccg tgcgaactgc
     1201 accagctcgg ccgcgtcgcg tccgtgcgcg atcaccagag tcccgcgctg cgcggtcgcg
     1261 ggaaataagc gccgccacca ggcgatgccc tcctccccaa gacgcgcgat gagcggacct
     1321 ccgctctcga gctcgcacca gggagcgagc atgccgccgg cgaaccagga gcaaccggcc
     1381 ccggcgagcg ccgccgcctg ctccagcacc tcgacggtgg cgccgcgctc ggcgagctcg
     1441 accgcgcagg cgagcccggc gaccccggcg ccccgcaccg tcacccgcat gccgctagcc
     1501 ctccttcgcg gccggtacgt acagctctga gccgccggcg cggaacttct cggccatctc
     1561 gcgcatcccc tgggcacgcg cctcggcgcg cacctcgtgc gagatcctca tcgagcagaa
     1621 tttcggtccg cacatcgagc agaaatgcgc gaccttgtgg ccctccttgg gcagcgtttc
     1681 gtcgtggaag gcgcacgccg tgtccggatc gagcgagagg ttgaactggt cctgccagcg
     1741 gaagtcgaac cgcgcccgcg acagcgcgtc atcgcgtgcc cgcgcggcgg gatgcccctt
     1801 ggcgagatcg gcggcgtgcg ccgcgatctt gtaggtgatg acgccggtct tgacgtcgtt
     1861 gcggtcgggg agcccgaggt gctccttggg ggtgacgtaa cagagcatcg cggtgccgta
     1921 ccagccgatc atggcggcac cgatcgcaga ggtgatgtga tcgtagccgg gggcgatgtc
     1981 ggtggtgagg ggtcctaagg tatagaacgg cgcctcgccg caggcggcga gctgcttgtc
     2041 catattctcc ttgatcttgt gcatcggcac gtgccctgga ccctcgatca tgacctgaca
     2101 gtcgcgcgcc cag
//
LOCUS       DATLKL010000072         2546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_839, whole genome shotgun sequence.
ACCESSION   DATLKL010000072 DATLKL010000000
VERSION     DATLKL010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..>2546)
                     /locus_tag="VM713_00970"
     CDS             complement(181..>2546)
                     /locus_tag="VM713_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82863.1"
                     /translation="WRMSEDDPVVFERITAMQATGSEPSQIRRVRANSNYITPKLGLF
                     SADTWSVLAIYVRNLILNWLMFLPLLIGCLLIPPWYVAVLKWAHSGNLHPGWPLIVGA
                     ALLTFALACAVWGRFRSRDKWVTQTRFLSLVLLPIVLSAAAFTFAAALGGLHAVLGGH
                     HHNLTEQYIVGAMLGVFLYAIAWLIGRIGAWRWDEKIIAWDLFCWILSGCVVGLIIAW
                     GMHLAAAARTFNPALIAILGLSGVVLAYLAGDIFYVGASSYSRRGEMDREWLARASGW
                     LAAVAVIWALVSVLSIFGSRLSAGLESVDTSFRALWTFVIGGGLSGLVTLVAGPHAGS
                     AATRAGTALERIPLTRIASVAAVIFAAILGVGIAWLGRGLLGLLAGCDSEIAVDEEAA
                     VILALIGLSFGISYFVNVNRFSMHALYRNRLVRAFLGSARRPGNRDPDPFTRFDAMDN
                     PRLAAVTPRSGPDRLFHVINTTLNVVATTNTAWAERKAESFTMTRQHCGNSFVGYQAT
                     ADYGDPRGGITLGTAMAISGAAVSPNQGYNSSPLVGFLLMLFNVRLGWWLGNPHFEKF
                     NREGPLFGIVPALKELAGLTTDRGPWIYLSDGGHFDNLGLYEMIRRRCRFIVAIDSGC
                     DPECKLEDLGNAVRKIYIDFGVSIEFAKFEIRARPKPQPCPPPGRPVVGARFATASIT
                     YPKTARDGPEPRTGWLLYIKPTYYETTEGVDVRGYASLHPSFPHESTTDQWFSESQLE
                     AYRALAEHIVEDVCCPHGKTREALKLRSLREAVERHTGHREAAEHRR"
BASE COUNT          499 a          817 c          834 g          396 t
ORIGIN      
        1 aggcgccgcg gcgatgtccg ccggctgagg aggactcgct ccccgcgtca cgtcggtacg
       61 gatcccgcgg gttggcacgc aagcgcccag cagtgccgcg agcgtgagga tcagtgctgc
      121 ccgaagcgct cccatgtcat acctccccga ctcctgcgag acccacgcgt agccgtttcc
      181 tcaacggcgg tgctcggcgg cctcgcggtg gcccgtgtgt cgctcaaccg cctctctcag
      241 ggagcgaagc ttcagcgcct cccgtgtttt gccgtgcgga cagcacacgt cctcgacgat
      301 gtgctccgcc agcgcccgat aagcctcgag ctgcgactcg ctgaaccact gatcggtggt
      361 gctctcgtgc gggaagctcg ggtgaagcga ggcgtagccg cgcacgtcga cgccttcggt
      421 cgtctcgtag taggtcggct tgatgtacag cagccagccc gtgcgcggct cggggccatc
      481 gcgggcggtc ttcggatagg taatggatgc cgtggcgaac ctcgcgccca cgaccgggcg
      541 gcccggcggc gggcacggtt gcggcttcgg tcgggcgcga atctcgaatt ttgcgaactc
      601 gatgctgacg ccgaaatcga tgtagatctt gcgaaccgcg ttgccgaggt cctcgagctt
      661 acactcggga tcgcagccag aatcaatcgc cacgatgaag cggcagcgcc gtcggatcat
      721 ctcgtagagg ccgaggttgt cgaaatgccc accgtccgag agatagatcc aaggcccccg
      781 atccgtggtc aggccggcaa gctccttgag cgcgggcacg atgccaaaga gtgggccctc
      841 gcggttgaac ttctcgaagt gcgggttgcc gagccaccag ccgagccgca cgttgaaaag
      901 catcagcagg aatccgacca gaggcgagga gttgtaaccc tgattggggc tgacggcggc
      961 gccggagatc gccatcgcgg tgccgagtgt gatgccgccg cgcggatcgc cgtagtccgc
     1021 ggtcgcctgg tagcccacga aggaattgcc gcaatgctgc cgggtcatcg tgaaggattc
     1081 cgccttgcgc tccgcccacg cggtattggt ggttgcgaca acgttgagcg tcgtgttgat
     1141 gacatgaaaa agccggtcgg gcccggagcg cggcgtgacc gccgccaacc tcgggttgtc
     1201 catcgcgtcg aagcgcgtga agggatctgg gtcgcgattg cccggacggc gcgccgagcc
     1261 gaggaaggcg cgcaccaggc ggttgcgata cagcgcatgc atcgagaagc gattgacgtt
     1321 cacgaagtag gagattccaa aggacagccc gatcagcgcg aggatcacgg ccgcctcctc
     1381 gtcgaccgcg atctcggagt cgcagccggc cagcagccca agcagccccc gtccgagcca
     1441 ggcaatgccg acgccaagga tcgccgcgaa gatgaccgcc gcgacactcg cgattcgcgt
     1501 cagcggtatg cgttcgagcg ctgtgccggc ccgggtcgcc gcgctccctg cgtgcggtcc
     1561 tgcgaccagg gttacgaggc ccgacaagcc gccaccgatt acaaaggtcc aaagtgctct
     1621 gaagctggtg tcgaccgact ccagcccggc actgagtcgc gacccgaaaa ttgacagcac
     1681 actcaccaag gcccagatga cggctaccgc ggccaaccat cccgacgccc gcgccagcca
     1741 ttcccggtcc atttcgccac ggcgcgagta actcgaggcg ccgacgtaaa agatgtcgcc
     1801 ggcgagatag gcgagcacga cgccactcag cccgaggatc gcgatgagag cgggattaaa
     1861 ggtccgtgcg gccgcggcaa gatgcatgcc ccaggcgatg atcagcccta ccacgcaccc
     1921 cgacaggatc cagcagaaca gatcccacgc aataatcttc tcgtcccatc tccaagcccc
     1981 gatccgtccg atgagccacg cgatggcata gaggaagacg ccgagcatcg ctccgacgat
     2041 gtactgctcg gtgagattgt ggtgatgccc gccgagcacc gcatgcaggc ctcccaatgc
     2101 agcggcgaac gtgaaggccg cggcggacag tacgatcggc aaaagtacca aggacagaaa
     2161 acgtgtctgg gtcacccact tgtcccgact cctgaagcga ccccagacgg cgcaggcgag
     2221 cgcgaaggtc agcagcgctg cgcctacgat gagtggccag ccgggatgta ggttaccgct
     2281 atgcgcccac ttgaggaccg caacatacca gggcggaatg agcaggcaac cgatgagcag
     2341 gggcagaaac atcagccagt tgaggatcag attacgcacg tagatggcga gcacggacca
     2401 ggtgtcggcg gaaaagaggc cgagcttcgg cgtgatgtag ttgctgtttg cacgaacgcg
     2461 gcggatctgt gatggctcgg agcccgtcgc ctgcatcgca gtgatccgct cgaatacgac
     2521 ggggtcgtcc tcggacatgc gccagg
//
LOCUS       DATLKL010000073         2077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_91077, whole genome shotgun sequence.
ACCESSION   DATLKL010000073 DATLKL010000000
VERSION     DATLKL010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91077"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VM713_00975"
     CDS             complement(<1..1031)
                     /locus_tag="VM713_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82864.1"
                     /translation="MRTSGSSTSIGVRRAAWPLLAWLAACSAVPPSAPASLICPYAPW
                     YGHAYRHGAVPTREAQQRILAWKASRREAQAPLSPATLVFGGGVDGIGVTSGDPRVYL
                     VFYGSQWRSGGDPRGAAGYLQRLFAGLGTGGEIWSGTMTQYCDGPTVPQGASTCNPAD
                     TPHVGYPQGGALAGVWFDTDTPAPASATPAQLAQEAIRAAAHFANSSPVANRYAQYLI
                     VSPSGTHPDGFGLADANFCAWHNYTTSPYGDLAYSNMPYVSDLGSSCGTSFVNPGAAG
                     ALDGFSIVGGHEYAETLTDQNPPGGWSTAAGAETGDECAWIAKGQGASADVTMGNGVY
                     AMQSTWSNDT"
     gene            complement(1070..>2077)
                     /locus_tag="VM713_00980"
     CDS             complement(1070..>2077)
                     /locus_tag="VM713_00980"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="HVH82865.1"
                     /translation="LNDKLDLAQAEAIADLIDAGSREAVRAAMRSLQGEFSAMVRGLT
                     EAVIELRTYVEAAIDFPEEEVDFLADRELTERFQAVREHFDGVLESARLGRLLREGMT
                     VVIAGRPNAGKSSLLNRLAGYDAAIVTPIPGTTRDVVRERIAIDGMPLHVLDTAGLRH
                     AGDPVEEEGMRRARAEIERADRVLFVIDAVEDPAAEAYREERARLPEDVPVTLVLNKC
                     DLAAGLPLADTVTGPPRLYVSALTGAGIEELRAHLKGCMGFQTVEGGAISARRRHLES
                     LARARGHTEEAARQLTERRAGELVAEELRAAQTALNEITGEFTSDDLLGRIFSSFCIG
                     K"
BASE COUNT          290 a          752 c          714 g          321 t
ORIGIN      
        1 gtgtcgttcg accaggtgct ctgcatcgcg tagacgccgt tgcccatggt gacgtccgcc
       61 gaggcgccct ggcccttggc gatccacgcg cattcatcgc ccgtttccgc cccggcggcc
      121 gtgctccagc cgccgggcgg attctgatcg gtgagtgttt cggcatactc gtggccgccg
      181 acgatggaaa agccgtcgag cgcgcccgcc gccccggggt tgacgaagct cgtcccgcag
      241 ctgctcccga ggtcgctgac gtagggcatg ttgctgtagg cgaggtcgcc gtacggggag
      301 gtggtgtagt tatgccaggc acagaagttc gcgtccgcca ggccgaagcc gtcgggatga
      361 gtgcccgagg gggaaacgat gaggtactgc gcatagcggt tggcgaccgg cgaggagttg
      421 gcgaagtgcg ccgccgcccg gatcgcttcc tgcgcaagct gcgcgggcgt cgcgctggcg
      481 ggcgccggag tgtcagtatc gaaccagact ccggcgagcg ctccgccctg cgggtagccg
      541 acgtggggag tgtctgccgg gttgcaggtg ctcgctccct gcggcacggt cgggccatcg
      601 cagtactgcg tcatggtacc cgaccagatc tcgccccccg tgcctagccc cgcgaacagg
      661 cgctgcaggt acccggcggc gccgcgcgga tcgccgccgc tcctccactg gctgccataa
      721 aacaccaggt acacgcgcgg gtccccgctc gtgacaccga tgccatcgac gccgccgccg
      781 aacaccagag tcgctgggct gagcggtgcc tgcgcttccc gccgagacgc cttccaggcg
      841 aggatcctct gctgcgcctc gcgcgtcggc accgccccgt ggcgataggc gtggccgtac
      901 cagggcgcgt acgggcatat gagacttgcg ggcgcggaag gcggcacggc gctgcaggcg
      961 gcgagccagg cgagcagcgg ccacgctgcc cggcgcactc cgatcgacgt cgagctccct
     1021 gatgtcctca tggcggtccc tcctcatctg tatgcgccgc cgggcgcgat tacttgccga
     1081 tgcaaaagct cgagaaaatt ctgccgagga gatcgtccga ggtgaactcg ccggtgattt
     1141 cgttgagcgc ggtctgcgcg gcacgcagct cctccgcgac cagctccccg gcgcgccgct
     1201 cggtgagctg ccgcgccgcc tcctcggtat gcccgcgggc gcgtgcgagg gactcgaggt
     1261 gccgccgtcg cgcgctgatc gccccgccct ccacggtctg aaagcccatg cagcccttga
     1321 ggtgcgcccg cagctcctcg atccccgcgc cggtgagcgc cgagacatac agacgcggcg
     1381 gaccggtgac cgtgtcggcg agcggcaatc ccgccgcgag atcgcacttg ttgagcacca
     1441 gcgtgaccgg cacgtcctcg ggcagccgcg cacgctcctc gcgataggcc tctgccgccg
     1501 ggtcctcgac cgcgtcgatg acgaacagca cccggtcggc gcgctcgatc tcggcgcgcg
     1561 ctcgccgcat gccctcctcc tcgaccgggt ccccggcgtg gcgcagcccc gccgtatcga
     1621 gcacgtgcag cggcatgccg tcgatcgcga tccgctcacg caccacgtcg cgcgtcgtcc
     1681 ccgggatcgg cgtgacgatc gcggcgtcgt agccggcgag tcggttcagg aggctcgact
     1741 tgccggcatt ggggcggccg gcgatgacga ccgtcatgcc ctcgcgcagc agccttccta
     1801 agcgcgcgct ctcgagcacg ccgtcgaagt gctcacgcac cgcctggaag cgctcggtga
     1861 gctcgcggtc ggcgaggaaa tcgacctcct cctccgggaa gtcgatcgcc gcctcgacat
     1921 aggtgcgcag ctcgatcacc gcctcggtca gcccgcgcac catcgccgag aactccccct
     1981 ggagcgagcg catggccgcg cgcaccgcct cgcgcgagcc ggcgtcgatg aggtccgcga
     2041 tcgcctcagc ctgggcgaga tcgagcttgt cgttgag
//
LOCUS       DATLKL010000074         1560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_158681, whole genome shotgun sequence.
ACCESSION   DATLKL010000074 DATLKL010000000
VERSION     DATLKL010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158681"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VM713_00985"
     CDS             <1..364
                     /locus_tag="VM713_00985"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HVH82866.1"
                     /translation="ATPAPDAANDDGTLAPTGALASMPYLPEASLAALKHYYRELGAE
                     LWGIYGPRDAYDPSTNWVSPIYMGLNQAPIVVMVENYRTGLPWKLFMSNPEIREMLRK
                     LDAATESAPRVTPLEPGK"
     gene            361..639
                     /locus_tag="VM713_00990"
     CDS             361..639
                     /locus_tag="VM713_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82867.1"
                     /translation="MTAAGGAEHAPGRRRMIAGLLAFGACGMSEAAAPGSTAARAAMA
                     AAASPLPQASIALDAFFDDLEKRTFDFFWETTNPANGLVPDRFPCVSE"
     gene            667..>1560
                     /locus_tag="VM713_00995"
     CDS             667..>1560
                     /locus_tag="VM713_00995"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HVH82868.1"
                     /translation="MGFGLTAYPIGVERGYITREAARARVLATLRFFAATGHEHGFFC
                     RFIDLKSGARVRHSEFSTVDTSLLLAGVLFCASYFDRDEPSERELRKLADDIYRRVDW
                     NWAQHRPPAISMGWTPEHGFIRTDWLGYCEAMLVYILALGSPTHPVGPDAWQAWTSTY
                     DPHWGTLYGQTHLSFAPLFGHQFSHVWIDFRGIRDAYMRQRDLDYFENSRRATYAQSS
                     YAIENPLQWRSYGENVWGLTACDGPGASRARALKRPGRFFRYMARGVGVEGVIDDGTI
                     APTAALGSLPFAPEIVIPAAHE"
BASE COUNT          246 a          545 c          495 g          274 t
ORIGIN      
        1 cgcgactccc gcacccgatg cggccaacga cgacggcacc ctcgcgccga ccggtgcgct
       61 cgcctcgatg ccctatctgc cggaagcctc actggcggcg ctcaagcact attaccgcga
      121 gctcggcgcc gagctctggg gcatctacgg gccgcgcgac gcgtacgacc cttcaaccaa
      181 ctgggtctca ccgatttaca tgggactcaa tcaggcgccg atcgtcgtca tggtcgagaa
      241 ctaccgcacc ggcctgccgt ggaagctgtt catgtccaac ccggagatcc gggagatgct
      301 gcgcaagctc gatgccgcca cggaaagcgc tccgcgcgtc accccattgg agccaggcaa
      361 gtgacggcgg ccggtggggc ggagcacgcg ccgggccgcc gccgaatgat cgcgggactg
      421 ctcgcgttcg gcgcctgtgg gatgtctgag gcagccgcgc ccggctccac ggccgctcgg
      481 gctgcgatgg ctgcggcggc gagcccgctg ccgcaagcgt ccatcgctct cgatgcgttt
      541 ttcgatgacc tcgagaaacg cacgttcgac ttcttctggg aaaccaccaa tccagccaat
      601 gggctggttc ccgatcgttt cccgtgtgtc tcggagtagg gtggttccgc atcgaacatc
      661 gccgcggtgg gcttcgggct caccgcttac ccgatcggcg tcgagcgcgg ctacatcacc
      721 cgggaggcgg cgcgagcgcg cgtgctggcg acgctgcgct tcttcgcagc cacgggccac
      781 gagcacggct tcttctgtcg tttcatcgac ctcaagagcg gcgcacgcgt acggcactcg
      841 gagttctcca ccgtcgacac gtcgctgctc ctcgccgggg tgctcttctg cgcgtcctac
      901 ttcgaccgcg acgagccatc cgaacgcgag ctcaggaagc ttgccgacga catctaccgg
      961 cgcgtcgact ggaactgggc ccagcatcgc ccgcccgcca tctcgatggg ttggacgccg
     1021 gagcatggtt ttatccgcac cgactggctg ggctactgcg aggcgatgct cgtctacatt
     1081 ctggcgctcg gctcccccac acatccggtg ggcccggatg cctggcaggc ctggaccagc
     1141 acctatgacc cgcactgggg cacgctctat gggcagacgc acttgagctt cgcgccgctc
     1201 ttcggacatc agttctcaca cgtgtggatc gatttccgcg gcatccgtga tgcctacatg
     1261 cgccagcgcg acctcgacta cttcgagaac agccgcaggg cgacctacgc tcagagcagc
     1321 tacgccatcg agaatccgct gcaatggcgc tcctacggtg agaacgtctg gggtctcacg
     1381 gcctgcgacg gacccggagc gtcgcgcgcg cgtgcgctga agagaccggg acggtttttc
     1441 cgctatatgg cccgcggcgt gggcgtggaa ggggtgatcg atgacggcac catcgctccg
     1501 accgcggcgc tcggctcgct gccctttgct cccgagatcg tgatcccggc cgcacatgag
//
LOCUS       DATLKL010000075         3330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_214962, whole genome shotgun sequence.
ACCESSION   DATLKL010000075 DATLKL010000000
VERSION     DATLKL010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214962"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3330
                     /gene="rpoC"
                     /locus_tag="VM713_01000"
     CDS             <1..>3330
                     /gene="rpoC"
                     /locus_tag="VM713_01000"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004666357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HVH82869.1"
                     /translation="KIGLASPEMIRAWSYGEVKKPETINYRTFKPERDGLFCAKIFGP
                     VKDYECLCGKYKRLKHRGVVCEKCGVEVTQAKVRRERMGHIELASPTAHIWFLKSLPS
                     RIGLMLDMTLREIERVLYFEAFVVIEPGMTPMQRGQLLTDEMYLEAIEEHGDEFDARM
                     GAEAVFELLRTIDLTAEIAKVREEMAGTSSETKLKRLSKRLKLIESFVESGNKPEWMV
                     MTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLRRLLELNAPDIIVRNEK
                     RMLQEAVDALLDNGRRGRAITGTNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSV
                     IVVGPQLRLHQCGLPKKMALELFKPFIFQKLQLRGEASTIKAAKRLVEREGPEVWDIL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPLLVEGKAIQLHPLVCTAFNADFDGDQMAVHV
                     PLSLEAQLEARALMMSSNNILSPANGDPIIVPSQDVVLGLYYMTRERIGARGEGMFFA
                     DVGEVHRAYESRGVDLQAKVRVRLRETLRGEGGTLTESIRVAETTVGRALLFEILPKG
                     LPFDLINQDMTKKAISATINACYRALGLKETVVFADQLMYTGFHYATRAGVSFGIDDI
                     VIPEQKTRIVTSADNEVKEIQDQYASGLVTNGERYNKVVDIWSRANDQVAKAMMEKLG
                     TEEATDSRGAHHKQKSFNSIFMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPIT
                     ANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDLVVTEIDCGTTN
                     GLAMTPLVEGGDVVEALGERVLGRVLAEDIVKPGAKEVLIPNGALLDEKMVRLLEHEG
                     VDQIKVRSPITCQTRYGVCSRCYGRDLARGRPISIGEAVGVIAAQSIGEPGTQLTMRT
                     FHIGGAASRAAAASSVEVRNKGLIRFHHVKTVSHEKGHLVAVSRSGELGVVDDFGRER
                     ERYKIPYGATISVKEGATVNAGQVVATWDPHTHPVITEVAGFVKFQDFVDGLTVTSQV
                     DEVTGLSSTVVLDTKQRGGKDLRATIKLVNGKGKEVTFANTEIPAVYTLPPGALVSLE
                     DGARVSVGDVIARIPQESSKRS"
BASE COUNT          600 a         1114 c         1105 g          511 t
ORIGIN      
        1 aagatcggcc tggcgtcgcc ggagatgatc cgggcctggt cgtacggcga ggtgaagaag
       61 cccgagacca tcaactaccg gactttcaag ccggagcgcg atggcctgtt ctgcgccaag
      121 atcttcggtc cggtgaagga ctacgagtgc ctgtgcggca agtacaagcg cctgaagcac
      181 cgcggcgtgg tgtgcgagaa gtgcggcgtc gaggtgacgc aggccaaggt gcgccgcgag
      241 cgcatgggcc acatcgagct cgcgagcccg accgcccaca tctggttcct caagtcgctc
      301 cccagccgca tcggcctcat gctcgacatg acgctgcgcg agatcgagcg ggttctctac
      361 ttcgaggcct tcgtggtcat cgagcccggc atgacgccga tgcagcgcgg gcagctgctc
      421 accgacgaga tgtacctcga ggccatcgag gagcacggcg atgaattcga cgcgcgcatg
      481 ggcgccgagg ccgtgttcga gctgctgcgc accatcgatc tcaccgccga gatcgccaag
      541 gtgcgcgagg aaatggccgg cacctcctcg gagaccaagc tgaagcgact ctcgaagagg
      601 ctcaagctca tcgagtcgtt cgtcgagtcg ggcaacaagc ccgagtggat ggtgatgacg
      661 gtgctgccgg tgctgccgcc ggacctgcgc ccgttggtgc cgctcgatgg cggccgcttc
      721 gccaccagcg acctcaacga cctctatcgg cgtgtcatca accgcaacaa ccgcctgcgc
      781 cggctcctcg agctcaacgc gcccgacatc atcgtgcgca acgagaagcg catgcttcag
      841 gaagcggtcg atgcgctgct cgacaacggc cgtcgcggcc gcgccatcac cggcaccaac
      901 aagcggccgc tcaagtcgct cgccgacatg atcaagggca agcaggggcg cttccggcag
      961 aacctgctcg gcaagcgcgt cgactactcg ggccgctcag tcatcgtggt aggtccgcag
     1021 ctgcggctgc accagtgcgg gctgccgaag aagatggcgc tcgagctctt caagcccttc
     1081 atcttccaga agctgcagct gcgcggcgag gcctcgacca tcaaggccgc caagcgcctg
     1141 gtggagcgcg agggaccgga ggtgtgggac atcctcgagg aggtcatccg cgagcatccg
     1201 gtgctgctga atcgcgcgcc gacgctgcac cgcctcggca tccaggcgtt cgagccgctg
     1261 ctcgtcgagg gcaaggcgat ccagctgcat ccgctggtct gcaccgcctt caacgccgac
     1321 ttcgacggtg accagatggc cgtccacgtg ccgctgtccc tcgaggccca gctcgaggcg
     1381 cgcgcgctca tgatgtcgtc caacaacatt ctctcgccgg cgaacggcga cccgatcatc
     1441 gtgccgtcgc aggacgtggt gctcgggctc tactacatga cgcgcgagcg catcggcgca
     1501 cggggcgaag gcatgttctt cgccgacgtc ggcgaggtgc atcgcgccta cgagagccgc
     1561 ggcgtggatc tgcaggccaa ggtgcgcgtg cgcctgcgcg agacgctgcg cggcgagggc
     1621 gggaccctta ccgagagcat ccgcgtcgcc gaaaccaccg tcggccgggc gctgctgttc
     1681 gagatcctgc cgaagggcct gcccttcgat ctcatcaacc aggacatgac caagaaggcg
     1741 atctccgcga ccatcaacgc ctgttatcgg gcgctggggc tgaaggagac ggtagtgttc
     1801 gccgaccagc tcatgtacac gggcttccac tacgccacgc gtgcgggcgt gtctttcggt
     1861 atcgacgaca tcgtcatccc ggagcagaag acccgcatcg tcacgtccgc ggacaacgag
     1921 gtgaaggaga tccaggacca gtacgcctcg ggcctcgtca ccaacggtga gcgctacaac
     1981 aaggtggtcg acatctggtc gcgcgccaac gaccaggtgg cgaaggcgat gatggagaaa
     2041 ctcggcaccg aggaggcgac cgactcgagg ggcgctcacc acaagcagaa gtccttcaac
     2101 tccatcttca tgatggccga ctcgggcgcg cgcggctccg cggcgcagat ccggcagctc
     2161 gccggcatgc gcggcctcat ggccaagccc gacggctcga tcatcgagac gccgatcacg
     2221 gcgaacttcc gcgaggggct gaacgttctg cagtacttca tctcgaccca cggcgcccgc
     2281 aagggcctcg cggacacggc gctgaagacc gcgaactcgg gctatctcac ccggcgtctg
     2341 gtcgacgtgg cgcaggacct ggtggtcacc gaaatcgact gcggcacgac caacggtctc
     2401 gccatgaccc cgctcgtcga gggcggtgac gtggtggagg cgctcggcga gcgcgtgctc
     2461 gggcgcgtgc tggcggaaga catcgtcaag cccggcgcca aggaagtact gatcccgaac
     2521 ggggcgctgc tcgatgagaa gatggtgcgc ctcctcgagc acgagggcgt cgaccagatc
     2581 aaggtccgct cgcccatcac ctgccagacc cgctatggcg tctgctcgcg ctgctacggg
     2641 cgcgacctcg cgcgcggccg gccgatctcg atcggcgagg cggtgggcgt cattgccgcg
     2701 cagtcgatcg gcgagccggg cacgcagctc accatgcgca ccttccacat cggtggtgcc
     2761 gcatcgcggg ccgcggccgc ttccagcgtc gaggtgcgca ataagggcct gatccgcttc
     2821 catcatgtga agaccgtgtc gcacgagaag ggacacctgg tggcggtgtc gcgctcgggc
     2881 gagctcggcg tggtcgatga cttcggccgc gagcgcgagc gctacaagat cccctacggc
     2941 gccaccatca gcgtcaagga aggggccacg gtgaacgcgg gccaggtcgt cgcaacctgg
     3001 gatccgcaca ctcacccggt catcaccgag gtcgcgggct tcgtcaagtt ccaggacttc
     3061 gtcgacggcc tcacggtcac ctcgcaggtg gatgaggtca ccgggctctc cagcaccgtg
     3121 gtgcttgaca ccaagcagcg cggcggcaag gacctgcgtg ccaccatcaa gctggtgaac
     3181 ggcaagggca aggaagtgac cttcgccaac accgagatcc cggccgtgta cacgctgcct
     3241 ccgggcgcgc tggtgtccct cgaggacggc gcgcgcgtct cggtgggcga tgtcatcgca
     3301 cgtatcccgc aggagtcctc caagagatcg
//
LOCUS       DATLKL010000076         1541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_23382, whole genome shotgun sequence.
ACCESSION   DATLKL010000076 DATLKL010000000
VERSION     DATLKL010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23382"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VM713_01005"
     CDS             <1..1314
                     /locus_tag="VM713_01005"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HVH82870.1"
                     /translation="PNRWFATQVLERLARESGHGARGALILVSLRNLRDINVSLGFSV
                     GDDVLREVAHRLRQNVSVNDTVARLGEAQFLVIVPGCTAERSLLYAEQLAGLIHTGFH
                     LADVGLVLHVACGVCLVPDHGRTTEELLQRAQVAIEDAHDGASHVAMYRPGQDDVHRR
                     RLTLVTDLSAAIDNDQLSLVYQPLVTMATRSVRSLEALVRWTHPQLGPVSPGEFVPLA
                     ERTGGVRRLTDWVLKSAIRQMGLWREEGLEMQLAVNLSAADILDPELGDQILALLRAH
                     SVHPTSLLLEITESAVMLDPQGAARNMQLLRIAGVRFSIDDFGTGHSSLSQLALLPVD
                     ELKIDRSLIGQAVHGNVTIVTSTIELGHSMGLKVVAEGVEDAEAWGLLRRLGCDFAQG
                     YLISPPLPVAQVAPFVREANQLLPASDATVLQLRALDQLAGRTKR"
BASE COUNT          216 a          547 c          523 g          255 t
ORIGIN      
        1 ccgaaccgct ggttcgccac ccaggtgctc gagcggctgg cccgcgagtc agggcacggc
       61 gcgcgcggtg cgctgatcct ggtgagcctg cggaacctgc gcgacatcaa cgtgtcgctt
      121 ggcttctcgg tgggggacga cgtgctgcgc gaggtcgccc accggctgcg ccagaatgtg
      181 tccgtgaacg acaccgtggc acgtctcggc gaggcacagt ttctggtgat cgtgccgggc
      241 tgcaccgccg agcgctcgct cctctacgcc gagcagctcg ccgggctcat ccacaccggc
      301 tttcacctcg cagacgtcgg cctcgtgctg cacgtcgcct gcggcgtctg cctggtgccc
      361 gatcacggcc gcaccactga agagctgctg cagcgcgccc aggtcgcgat cgaggacgcg
      421 cacgatgggg cctcgcacgt cgccatgtac cgcccgggcc aggacgacgt gcaccgccgt
      481 cggctgaccc tggtcaccga cctgagcgcg gccatcgaca acgaccagct gtcgctggtg
      541 tatcagccgc tcgtcaccat ggcgacgcgc tcggtgcgca gcctcgaggc tctggtgcgc
      601 tggacccacc cgcagctcgg acccgtctcc cctggggagt tcgtgccgct cgccgagcgc
      661 accgggggcg tgcgccggct caccgattgg gtgctgaagt ccgccatccg ccagatgggg
      721 ctgtggcgcg aggagggcct cgagatgcag ctggccgtca acctgtcggc cgcggacatc
      781 ctcgacccgg agctcggcga ccagatcctc gcgctgctgc gcgctcactc ggtgcatccc
      841 acttcgctcc tgctcgagat caccgagagc gcggtgatgc tcgatccgca gggcgcggcg
      901 cgcaacatgc agttgctgcg catcgccggt gtgcgcttct ccatcgacga cttcggcacc
      961 ggccactcct cgctctcgca gctcgcgctc ctgccggtgg acgagctgaa gatcgaccgc
     1021 tcgctcatcg gtcaggcggt gcacggcaac gtgacgatcg tcacttcgac catcgagctc
     1081 ggccacagca tgggactcaa ggtggtcgcg gagggcgtcg aggacgccga ggcctggggc
     1141 ctgctgcgcc gcctgggatg cgatttcgcg cagggctatc tcatcagccc accgctgccg
     1201 gtggcgcagg tggcgccgtt cgtgcgcgag gccaaccagc tgctgccggc ctcggacgcc
     1261 acggtgctgc agctgcgggc gctcgatcag cttgccgggc gcaccaagcg ctaacaacga
     1321 agacgcagcc ggcgaaagcc cgtgcgccga cctagctacg gagtgctcgg ctgcggaggt
     1381 gattcggact cgtgcgtatc gagccccgct ttttgccggc tgcgcagcag ggagcgattt
     1441 cgtcggacat taccctgatt tccgccttcg ttgttagcgc ttggtgcgcc cggcaagctg
     1501 atcgagcgcc cgcagctgca gcaccgtggc gtccgaggcc g
//
LOCUS       DATLKL010000077         1760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_79735, whole genome shotgun sequence.
ACCESSION   DATLKL010000077 DATLKL010000000
VERSION     DATLKL010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79735"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VM713_01010"
     CDS             <1..1304
                     /locus_tag="VM713_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HVH82871.1"
                     /translation="RRPDMAALFEAILAHVPPPAAQEELPLQLQISTLDYNSYVSRIG
                     IGRIRRGSVRPGQTVALRYGNADRGEAKIGQVLTFTGLERLPVEEASAGDIVAVTGVE
                     AVNIGLTVCDREHPEGLPPIAIDEPTLQMNFQVNSSPLAGREGKYVTSRQLRERLYRE
                     LQSNVALRVEDTDEPDVLRVSGRGELHLTILIENMRREGYELAVSRPHVLTKLVDGEV
                     HEPYEALTVDLEEAHQGAVMEALGTRRAELSDMSVDGNGRVRLEYRVPARGLIGFQGE
                     FLTLTRGTGLISHIFDGFAPVKGEIPDRHNGVLVSNENGEAVAYALFNLQERGRLFVS
                     PGEKLYVGMIVGIHSRDNDLVVNPIKTKKLTNIRAAGKDEAILLTPPIELTLEYAVEF
                     IAEDELVEVTPTSIRIRKRFLKEQERKRAARADAAAATGTA"
     gene            1334..>1760
                     /locus_tag="VM713_01015"
     CDS             1334..>1760
                     /locus_tag="VM713_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82872.1"
                     /translation="MTSDLRTFEGSCHCGALGFSFQTALPVAEWSVRACQCAFCRAHG
                     ALTTSDPSGELAFQVRRDEALQRYRFGLRTADFLLCRSCGIYLGAQLVTAQGPFGIIN
                     TRALLPLPESLPAAVEADYACESASDRVARRARRWTPLSR"
BASE COUNT          290 a          614 c          598 g          258 t
ORIGIN      
        1 agcgccgccc ggacatggcg gcgctgtttg aggcaatcct cgcgcacgtg ccaccgccgg
       61 ccgcgcagga ggagctgccg ctgcagctgc agatctcgac cctcgactac aactcctacg
      121 tcagccgcat cggcatcggc cgcatccgcc gcggctcggt gcggcccggg cagacggtcg
      181 cgctgcgtta cggcaacgcc gatcgcggcg aggcgaagat cggtcaggtg ctgaccttca
      241 ccggtctcga gcggctgccg gtcgaggaag cgagcgcggg cgacatcgtc gccgtcaccg
      301 gcgtcgaggc cgtcaacatc gggctcacgg tgtgcgatcg cgagcacccc gaggggctgc
      361 cgccgatcgc catcgacgag ccgaccctgc agatgaattt tcaggtcaac agctcgccgc
      421 tcgccgggcg cgagggcaag tacgtcacca gccggcagct gcgcgagcgg ctgtaccgcg
      481 agctgcaatc gaacgtcgcg ctgcgcgtcg aggacaccga cgagccggac gtgctgcgcg
      541 tttcggggcg cggcgagctg cacctcacca tcctcatcga gaacatgcgc cgcgagggct
      601 acgagctcgc cgtctcgcgc ccacacgtcc tcaccaagct cgtggacggc gaggtgcacg
      661 agccctacga ggccctcacc gtggacctcg aggaggcgca ccagggagcg gtgatggagg
      721 cactcggcac gcgccgcgcc gagctttccg acatgagcgt cgacggcaat gggcgcgtgc
      781 ggctcgagta ccgcgtgccg gcgcgcggcc tcatcggctt ccagggcgag ttcctcacgc
      841 tcacgcgcgg cacggggctc atcagccaca tcttcgacgg cttcgcgccg gtgaagggcg
      901 agattcccga ccgccacaac ggcgtgctcg tgagcaacga gaacggcgaa gcggtggctt
      961 acgccctctt taacctgcag gagcgggggc ggctgttcgt cagcccgggc gagaaactct
     1021 acgtgggcat gatcgtcggc atccacagcc gcgacaacga cctggtcgtg aaccccatca
     1081 agaccaagaa gctcaccaac atccgcgccg ccggcaagga cgaggcgatc ctgctcaccc
     1141 cgccgatcga gctcactctc gagtacgcgg tcgagttcat cgccgaggac gagctcgtcg
     1201 aggtgacgcc cacctcgatc cgcatccgca agcgcttcct caaggagcag gagcgcaagc
     1261 gcgcggcgcg cgccgacgcc gccgcggcca cgggtaccgc gtagcaacct caggtgccgc
     1321 gaggggcgct gccgtgacgt ccgacctgcg caccttcgag gggagctgcc attgcggagc
     1381 actcggattc tcctttcaaa cggcactccc ggtggctgag tggagcgtgc gcgcctgcca
     1441 gtgcgccttc tgtcgcgctc acggtgccct gacgacatcc gacccaagcg gcgagctcgc
     1501 gttccaggtc aggcgggacg aggcgctgca gcgctatcgg ttcggcctga ggaccgcgga
     1561 tttcctcctg tgcagatcct gtgggattta tctcggtgcg cagctcgtga ccgcacaggg
     1621 cccgttcggg atcatcaaca cgcgggcgct gctcccgctg cccgagagtc ttccggccgc
     1681 ggtagaggcg gattacgctt gtgaaagcgc gagcgatcga gtcgcgcggc gcgcgcggcg
     1741 gtggactccg cttagcaggc
//
LOCUS       DATLKL010000078         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_12066, whole genome shotgun sequence.
ACCESSION   DATLKL010000078 DATLKL010000000
VERSION     DATLKL010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12066"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VM713_01020"
     CDS             complement(<1..186)
                     /locus_tag="VM713_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82873.1"
                     /translation="MASPAPRLQLRALGLRVSGRLASRRAAPRVPEQPVLRLPAVGVQ
                     WPAAGARRRRRALGLALE"
     gene            210..1043
                     /locus_tag="VM713_01025"
     CDS             210..1043
                     /locus_tag="VM713_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82874.1"
                     /translation="MLTFDRVFFAVIVLLLAVGLKFPTQRYITPVRGVGYWLGIIGGS
                     MMVLLLVYSVRKRVSWLSWMGSTPAWFRFHMVLGVVGPLCILYHSNFSTGATNSNVAL
                     FAMLTVAGSGFIGRYFYAHVHYGLYGRKLELGDLRSGADKLRCLSGRMSFVPDLVKRI
                     EASEQRLLDSGPQLSMLGFLKPLVVAAATARERRRLESYVRSALKAVARTSPAVATQR
                     RPLTRAACLYIDRRLTATKRVAEFSGYERLFSLWHALHIPLIFALAIAAVIHVIAVNI
                     Y"
     gene            1060..>2080
                     /locus_tag="VM713_01030"
     CDS             1060..>2080
                     /locus_tag="VM713_01030"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HVH82875.1"
                     /translation="MQEGRWRTLLIFGLLTGLVPARHAEAVNPEKLLMPGNVIAAHAK
                     WEEQCTQCHDRGAANPGARQTVLCLDCHKEINADIQRQRGYHGHLPGIAETQCSACHV
                     EHLGRGADIAKLVPEQFNHDLTDYKLRGAHIDVPCGSCHVHGMKYRDATRDCLACHKK
                     EEPHEGKLGKDCGSCHNDSAWRQVHYDHDKTAFPLRDKHAEIACAACHFGNRYKDTPK
                     ECVSCHEPDDAHNGKRGVKCADCHTTKSFKTQKFDHEKESGGFALLGAHGRIACNDCH
                     RSGVMKDKIPKDCFGCHQGEVSHAGRMGKDCGKCHDSEKWKPAPKFDHTRDGKWPLEG
                     RHQKIA"
BASE COUNT          368 a          723 c          668 g          321 t
ORIGIN      
        1 ctccagcgcc aaccccagcg cgcggcgccg ccgtcgcgcc ccggccgccg gccactgcac
       61 cccgaccgcc ggcagccgca acaccggctg ctcgggcacc cgtggcgccg ccctcctcga
      121 cgccagcctc cccgacaccc gtagcccgag cgcccgcagc tgcagccgcg gcgccggcga
      181 tgccacaacc tccggcgcaa gcccagggga tgctgacttt cgaccgggtc ttcttcgccg
      241 tgatcgtgtt gttgctcgct gtcgggctga agttcccgac ccagcgctac atcaccccgg
      301 tgcgcggtgt gggttactgg ctcggcatca tcggcggcag catgatggtg ctgctgctgg
      361 tgtactcggt gcgcaagcgg gtgagctggc tgagctggat gggctcgaca cccgcgtggt
      421 tccgcttcca catggtgctc ggcgtggtcg gaccgctgtg cattctctat cactcgaact
      481 tcagcactgg cgcgaccaac agcaacgtcg cgctcttcgc catgctgacc gtcgcgggca
      541 gcggcttcat tggccgttat ttctacgcgc acgtacacta cggcctgtat ggtcgcaagc
      601 tcgagctcgg tgacctgcgc tctggggctg acaagctgcg ttgtctctcc gggcgcatga
      661 gcttcgtgcc ggacctcgtc aagcgcatcg aggcgtcgga gcaacggctc ctcgacagcg
      721 gaccccagct ctccatgctg gggtttctca agccactggt ggtcgcggcc gcgacggcgc
      781 gggagcgccg gcggctggag agctacgtgc gcagcgcgct caaggcggtg gcacgcacct
      841 cgccggcggt tgcgacgcag cgccggcccc tgacgcgcgc tgcctgcctg tacatcgacc
      901 gccgcctcac cgccaccaag cgcgtggccg aattctccgg ctacgagcga ctgttctcgc
      961 tctggcacgc gctgcacatt cccctgatct tcgcacttgc aatcgccgcg gtgatccacg
     1021 tgatcgcggt caacatttac tgacgaccac tcgacccaca tgcaggaggg gcgatggcgc
     1081 acgctgctga tattcggact gctgacgggg ctggtgccgg cgaggcacgc tgaagccgtg
     1141 aacccggaga agctcctcat gccgggcaac gtgatcgctg cgcacgccaa gtgggaagag
     1201 cagtgcacgc agtgccatga ccgcggcgcc gccaaccctg gggcgcgcca gacagtgctg
     1261 tgcctcgatt gtcacaagga gatcaacgcc gacattcagc ggcagcgggg ctaccacggc
     1321 cacctcccgg gcatcgccga gacccagtgc agcgcctgtc acgtggagca cctcggccgc
     1381 ggcgccgaca tcgcgaagct ggtacccgag cagttcaacc acgacctcac cgattacaag
     1441 ctgcgcgggg cgcatatcga cgtgccatgc ggcagctgtc acgtccacgg catgaagtac
     1501 cgcgatgcga cgcgggactg cctcgcctgc cacaagaaag aggagccgca tgagggcaag
     1561 ctcggcaagg actgcggctc ctgccacaac gacagcgcct ggcggcaggt gcattacgac
     1621 cacgacaaga ccgccttccc gctgcgcgac aagcatgcgg aaatcgcctg tgccgcctgt
     1681 cacttcggca accgctacaa ggacacgccg aaggaatgcg tctcctgcca cgagccggac
     1741 gacgcgcaca acggcaagcg cggcgtgaag tgcgcggact gccataccac caagtcgttt
     1801 aagacccaga agttcgatca cgagaaggag agcggcggct tcgcgctcct cggggcgcat
     1861 ggccgcatcg cctgcaacga ctgccatcgc agcggcgtca tgaaggacaa gattccgaag
     1921 gattgcttcg gctgtcacca gggcgaggtt tcgcacgccg ggcggatggg gaaggactgc
     1981 ggcaagtgcc acgacagcga gaagtggaag ccggcgccga agttcgatca cacgcgtgac
     2041 ggcaaatggc cgctcgaggg ccgacaccag aagatcgcct
//
LOCUS       DATLKL010000079         2634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_214999, whole genome shotgun sequence.
ACCESSION   DATLKL010000079 DATLKL010000000
VERSION     DATLKL010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214999"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VM713_01035"
     CDS             complement(<1..991)
                     /locus_tag="VM713_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HVH82876.1"
                     /translation="MRRLTTLTLTCLLACTALGAAAQDPADNPGMAGEGAAGESRFDV
                     MVDNAPAQPFFQGLVAGTSYNMLVHPEVGGRISLTLKHVTIEEVLEAARDLYGYDYRR
                     TRTGYMVLPAAVQSRVFHLSYLDLKRFGVSNTRISSGQVTQSGNNSTYGNTVSGGAQS
                     STQTATDPTGRPLDVTGTSVVTRGDSDFWAGIEADLHALIGAKPGRSVVINRQSGLIV
                     VHAMPNELRDVAEYLEKTAHTVTRQVVLEAKIVEVDLSDGYQAGINWASLLQRGASTY
                     LLGQSAPLNGFQVNPFTTGPAANPYLPPSASGGVNVQPGTSTPLTGFVSNPLSG"
     gene            complement(988..1353)
                     /locus_tag="VM713_01040"
     CDS             complement(988..1353)
                     /locus_tag="VM713_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82877.1"
                     /translation="MRVLARIVALFAVAVLGADTARAGNLLVDPTRPMTAADSPVAAP
                     ESSGVHVQAIVIRADSRVAVVDGRVVRAGDRIAHVYIEEVTPEGVRYLQDGHSRFARL
                     KCPTPLPVRRGAAPQRDLP"
     gene            complement(1350..1991)
                     /locus_tag="VM713_01045"
     CDS             complement(1350..1991)
                     /locus_tag="VM713_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82878.1"
                     /translation="MRVPAYSVLAGRFDRLTLRERLLVLAAAIAVAVGAFKILLLGPL
                     DARGAQLSQQLSVLETGINETARASAADSSTAAYARAADLRARLAAVNAELSSQSAGM
                     IAPQRMVEVIHDVLSRQQGVVLVSMRNLEAIKLPLDAAGESATGTPRPYEHTIEIVLE
                     GRYLDVLAYLQALEALPWRFYWRRLEMTTTRYPLNRVRVELGTVSMGSEWIDL"
     gene            complement(1988..>2634)
                     /locus_tag="VM713_01050"
     CDS             complement(1988..>2634)
                     /locus_tag="VM713_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82879.1"
                     /translation="PAAQSSVAPFLAHSVLLVSGLAVSCLLAVWAYGWWRVARLERAV
                     HSLEQQQHQQDASLVALSSARGADAPPEQMQARVAMLTAELAVRTRTLELLRSGTAGP
                     VGGFSTRLAALAHRPTPGLWIDHVVLSGMTDSMTVGGTALEPDLVPRYLRGLAEEGAL
                     AGARFDELVIERPATGHADDPGQATAEKAKPDHSFRFRAESGALRAPPATEKSS"
BASE COUNT          410 a          894 c          905 g          425 t
ORIGIN      
        1 cgccgctcaa ggggttcgag acgaagcccg tgagcggtgt gctggtgccg ggctgcacgt
       61 tcacgccgcc gctcgcggat ggtggcagat agggattggc cgccggcccg gtggtgaagg
      121 gattcacctg gaagccgtta agcggcgcgc tctgacccag gagataggtg ctggcgcccc
      181 gttgcagcag cgaggcccag ttgatgcccg cctgatagcc gtcgctcaga tccacttcga
      241 cgatcttcgc ttcgagcacc acctgccggg tcacggtgtg cgccgtcttc tcgagatact
      301 cggcgacgtc gcgcagctca ttgggcatgg cgtgcacgac gatgaggccc gactggcggt
      361 tgatgaccac gctgcgcccg ggcttggcgc cgatgagcgc atgcagatcg gcctcgatcc
      421 ccgcccagaa atccgagtcg ccccgggtca cgaccgaggt cccggtcacg tcgagcgggc
      481 ggccggtcgg gtcggtggcc gtctgggtcg agctctgcgc gccaccgctc acggtgttgc
      541 cgtaggtcga gttgttaccg ctctgggtca cctgtcccga gctgatgcgc gtgttggaga
      601 ccccgaagcg cttcagatcg aggtagctga ggtgaaatac gcgcgactgt accgcggccg
      661 gcagcaccat gtaccccgtt ctggtgcgcc ggtagtcgta gccatagagg tcgcgcgcgg
      721 cttccagcac ttcctcgatg gtgacgtgct tgagcgtcag gctgatgcgc ccgcccacct
      781 cgggatgaac cagcatgttg tagctggtgc ccgcaacgag cccctggaag aacggttgcg
      841 ccggggcgtt gtcgaccatg acgtcgaagc gcgactcgcc agcggcgccc tcgcccgcca
      901 tgccgggatt gtccgccggg tcttgggctg ccgcgccgag agcggtgcag gccagcaggc
      961 aggtcagggt cagcgttgtc agtcgtctca tggcagatct cgttggggtg cggcgccgcg
     1021 gcgcacgggg agcggtgtcg gacatttgag tcgcgcgaac cggctgtgac cgtcctgtaa
     1081 atagcggacg ccctctggcg tcacctcttc gatgtatacg tgcgcgatgc gatccccggc
     1141 gcgtaccacg cggccgtcca ccactgcgac ccgcgagtcg gcgcggatga cgatcgcctg
     1201 cacgtgcacc ccactagact ccggtgccgc gaccggggag tcggccgccg tcatgggccg
     1261 ggtcggatcc acgagcaaat tgccagcgcg cgctgtgtcg gcgccgagca ccgcgaccgc
     1321 aaacagggca acgatgcgag ccagcactct cacaggtcga tccattcact gcccatgctc
     1381 acggtgccga gctcaacccg gacccggttg aggggatagc gcgtcgtggt catctcgagg
     1441 cgccgccagt agaagcgcca cggcagggcc tccagtgcct ggaggtaggc gagcacgtcc
     1501 agatagcggc cttcgagaac gatctctatg gtgtgctcgt acggccgggg cgtgccggtc
     1561 gcgctctcgc cagccgcatc gagcggcagc ttgatggctt cgagattacg catgctcacg
     1621 agcaccacgc cctgctggcg gctcaggaca tcgtgtatga cttccaccat gcgctgcggc
     1681 gcgatcattc cagccgactg cgagctcagc tcggcattca cggcggcgag gcgcgccctg
     1741 agatcggccg cccgggcgta ggcggccgtg ctggaatccg cggcagacgc cctggcggtc
     1801 tcattgattc cagtctcgag taccgagagc tgctgtgaga gctgcgcacc gcgcgcatcc
     1861 aacgggccga ggagcaggat cttgaaggcg cccaccgcta cggcaatcgc ggccgcgaga
     1921 accaagagcc gctcccgcag ggtcagccga tcgaagcgac cagccaacac gctgtaggcc
     1981 ggaaccctca cgaggatttc tccgtggcgg gaggcgcacg caacgcgcca ctctcggcgc
     2041 ggaacctgaa gctgtgatcg ggcttggctt tctccgctgt ggcctggccc gggtcgtctg
     2101 cgtggcccgt ggccggccgc tcgatcacga gctcatcaaa gcgcgctcct gcgagcgcgc
     2161 cttcttcggc caacccgcgc aggtagcgcg gcacgaggtc gggctcgagc gccgtgccgc
     2221 cgacggtcat cgagtcagtc atgcccgaca agaccacatg atcgatccac agtcccggcg
     2281 tcggccggtg cgcgagcgct gcgagccgcg tggagaaccc gccgaccggt cctgccgtgc
     2341 cgctgcgcag cagctcgagc gtgcgcgtgc gtacggcaag ctccgctgtc agcatggcca
     2401 cgcgcgcctg catctgttcc gggggcgcgt ccgcgccgcg cgccgagctc agtgcgacca
     2461 ggctggcgtc ctgctggtgc tgctgttgct cgagcgagtg cacggcgcgc tccagacgcg
     2521 cgacccgcca ccagccgtag gcccacactg cgagcaggca gctcacggcg agcccgctga
     2581 ccaacagcac gctatgtgcc aagaacggcg cgacgctcga ctgtgcggcc ggct
//
LOCUS       DATLKL010000080         1725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_215013, whole genome shotgun sequence.
ACCESSION   DATLKL010000080 DATLKL010000000
VERSION     DATLKL010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215013"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1328
                     /locus_tag="VM713_01055"
     CDS             75..1328
                     /locus_tag="VM713_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVH82880.1"
                     /translation="MQPRIAGRNFLFVPGPTNIPDRVQRAMMVAMEDHRSSKFPDLSR
                     SVLDDLKRVFKTTMGQVFIFPASGTGAWEASLTNTLSPGDRLLAARFGQFSHLWIDMA
                     QRLGYEVEVLDVAWGEGVPLARYEEILRADKRHEIKGVLACHNETATGVTSDIAGVRR
                     ALDAAGHPALLFVDAVSSLASIDFRMDEWRVDLAVSGSQKGLMLPAGLGILAVSPKAL
                     AAVAGARSRRCYFDLNDMIKANATGYFPYTPALPMLYGLRESLNLIFEEGLEQIFFRH
                     HYLAEGVRAAVTRGWRLALCAREPQWYSDTVSAVVVPEGINGAHVIDVAFRRYNLALG
                     AGLAKVAGKVFRIGHLGDLNELMLMGAIAGAEMAMLDVGIGVTPGSGVAAASDYWRSH
                     DPIPRKKVSQQEQFYESHSTGSIQG"
     gene            1330..>1725
                     /locus_tag="VM713_01060"
     CDS             1330..>1725
                     /locus_tag="VM713_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82881.1"
                     /translation="MREHIVFLDRSTLKARVRSPGFEHDWQEYAVTGASEIVPRLRAA
                     TVAITNKVPLRADALRQLPRLRMIAVAATGYDVIDLDYCREHGLAVANIRNYALHTVP
                     EHTFALITALRRNLLAYRRDVEQGRWQQAE"
BASE COUNT          282 a          586 c          580 g          277 t
ORIGIN      
        1 cgtccccgac ggctgccgca acgtggccag aagggcctag actcggccga ggcgattgtc
       61 atagggagcg cgcgatgcaa cctcgcatag cagggcgaaa cttcctgttc gtcccgggcc
      121 ccacgaacat tcccgaccgc gtgcagcgcg ccatgatggt agcgatggag gaccatcgct
      181 cctcgaagtt cccggatctc tcgaggagcg tcctggacga cttgaagcgc gtcttcaaga
      241 cgaccatggg ccaggtcttc atcttcccgg cctcgggcac cggcgcctgg gaagcatcgc
      301 tcaccaacac cctttcgccg ggcgaccggc tcctggccgc gcgcttcgga cagttcagtc
      361 acctgtggat cgacatggcg cagcgcctcg gctacgaggt cgaggtactc gacgtcgcct
      421 ggggcgaggg cgtgccgctg gcgcgctacg aggagattct gcgcgcggac aagcggcacg
      481 agatcaaggg cgtgctggcc tgccacaacg agacggcgac gggcgtcacc agcgacatcg
      541 ccggtgtgcg ccgcgcgctg gatgccgccg gacatccggc gctgctgttc gtggacgccg
      601 tgagctcgct cgccagcatc gatttccgca tggacgagtg gcgcgtcgac ctcgccgtca
      661 gcggctcgca gaaggggctg atgctgccgg cgggcctcgg catcctggcg gtgagcccga
      721 aggccctggc cgcagtcgcg ggcgcccgct cgcggcgctg ctacttcgac ctcaacgaca
      781 tgatcaaggc caacgccacc ggctacttcc cctatacccc ggcgttaccg atgctgtacg
      841 gcctgcgtga gtcgctcaac ctcatcttcg aagaggggct cgagcagatc ttcttccggc
      901 accactatct ggccgagggt gtgcgcgcgg ccgtgacgcg cggctggcgc ctcgcgctct
      961 gtgcgcgcga gccgcagtgg tattccgaca cggtgagcgc cgtcgtcgtg cccgagggca
     1021 tcaacggcgc gcacgtgatc gatgtcgcgt tccgccgcta caacctggcg ctcggcgcgg
     1081 gactcgccaa agtggccggc aaggtgttcc gcatcgggca cctgggagat ctcaatgagc
     1141 tgatgctgat gggcgcgatc gccggcgccg agatggccat gctcgacgtc ggtatcggag
     1201 tcacgcccgg gagcggcgtc gccgccgcct ccgattactg gcgctcgcac gatccgattc
     1261 ccagaaagaa ggtcagccag caggagcagt tctacgaatc gcattcgacc gggtcgattc
     1321 agggctgaga tgcgcgaaca catcgtcttt ctcgatcgct cgacgctcaa ggcgcgcgtg
     1381 cgcagcccgg gcttcgagca cgattggcag gaatacgcgg tcaccggcgc gagcgagatc
     1441 gtgccgcggc tgcgcgcggc gaccgtggcg atcaccaaca aggtaccgct gcgcgccgac
     1501 gcgctgcgcc agttgccgcg gctcaggatg atcgcggtgg ccgcgaccgg ttacgacgtc
     1561 atcgacctcg actattgccg ggagcacggc ctcgcggtcg ccaacatccg caactacgcg
     1621 ctgcacacgg tgccggaaca taccttcgcg ttgatcacgg cgctgcgccg caatctgctc
     1681 gcctaccggc gcgacgtcga gcagggcagg tggcagcagg ccgag
//
LOCUS       DATLKL010000081         3248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_102390, whole genome shotgun sequence.
ACCESSION   DATLKL010000081 DATLKL010000000
VERSION     DATLKL010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102390"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1442)
                     /gene="hslU"
                     /locus_tag="VM713_01065"
     CDS             complement(81..1442)
                     /gene="hslU"
                     /locus_tag="VM713_01065"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HVH82882.1"
                     /translation="MASALTPRQIVQELDKHIIGQDAAKRAVAIALRNRWRRMQVEEP
                     LRQEITPKNILMIGPTGVGKTEIARRLAKLANAPFVKVEATKFTEVGYVGRDVDSIVK
                     ELADVAVKITREQEMAKVRERALDAAEERVLDTLLPKPRMMGFSTDEPSHARDSETRQ
                     KFRKMLREHELDDREIEIEVRALPMGVEIMAPPGMEEMQQQLQSMFQNLSGNRTRTRK
                     VKVREALKLLIDEEAGKLINEDELKIQAHANAEQNGIVFIDEIDKVTRRQETIGADVS
                     REGVQRDLLPLVGGSTVATKYGPVKTDHVLFIASGAFSLSKPSDLIPELQGRLPIRVE
                     LDSLNVGDFVRILTEPDASLTAQYQALMATEGVAVEFAADGVRRIAEIAFEVNERTEN
                     IGARRLHTVMERLLESVSYEAAADDTAGGNPGTSPVTIDARYVDNHLGALVKDEDLSR
                     YIL"
     gene            complement(1447..2019)
                     /gene="hslV"
                     /locus_tag="VM713_01070"
     CDS             complement(1447..2019)
                     /gene="hslV"
                     /locus_tag="VM713_01070"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HVH82883.1"
                     /translation="MSDPTVFNPHGTTILGVRRNGAVALGGDGQVTLGSTVMKGNARK
                     VRRLFGDKVLAGFAGGTADAFTLFERFEGKLEKYGNLTRAAIELAKDWRSDRYLRRLE
                     ALLLVGDPEKLFIISGNGDVIEPDHDVAAIGSGGQFALAAARALLEGSDLPARAIVER
                     SLDIAADICIYTNRSITIEELNAAKGRESK"
     gene            complement(2067..3011)
                     /gene="xerC"
                     /locus_tag="VM713_01075"
     CDS             complement(2067..3011)
                     /gene="xerC"
                     /locus_tag="VM713_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008040629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HVH82884.1"
                     /translation="MSPAALEWLARFRRYLASERRLSTHTDAAYARDLGTLVKFCDQH
                     GLAKWSALDSQHVRSFAAHSHAGGLSPRSIQRRLSAVRSFYEFLLREAPAEAASGKRS
                     RRGARVRANPALEVRAPKAARRLPHTLDADQMGRLLQIPAGGGLTARDRALMELLYSS
                     GLRLAELVGLDLADLDLRDRTVRVLGKGRKARIVPVGRLALAALRAWLKERAALAPMD
                     EPALFVARTGQRLGPRAIQARVSYWAKRQGLGVHVHPHLFRHSFASHLLESGGELRGV
                     QELLGHADISTTQIYTHLDFQHLARTYDAAHPRARRKP"
     gene            complement(3043..>3248)
                     /locus_tag="VM713_01080"
     CDS             complement(3043..>3248)
                     /locus_tag="VM713_01080"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HVH82885.1"
                     /translation="SQREFLFGPEGPEMGSVALVPLGAKGSIGLLALGSVDRDRFHPG
                     MSTEFLARMADLIADALTATEAA"
BASE COUNT          462 a         1082 c         1175 g          529 t
ORIGIN      
        1 cgcggcatac aaatgacgca aaggttcaag cggcgcggcg gcgccacccg ggggggattg
       61 gggccccccg tgggcgcccc ctagaggatg tagcggctca ggtcctcgtc cttgacgagc
      121 gcacccaggt ggttgtcgac atatctggcg tcgatggtca ccggggaggt gccggggttg
      181 ccgccggccg tgtcgtcggc tgcggcctcg taggagacgc tctcgaggag gcgctccatc
      241 accgtgtgca gacgccgggc gccgatgttt tcggtgcgct cgttgacctc gaaggcgatc
      301 tcggcgatgc ggcgcacgcc gtcggcggcg aattcgaccg cgacgccctc ggtcgccatc
      361 agcgcctggt actgcgcggt gagggaggcg tcgggctcgg tgaggatgcg tacgaagtcg
      421 cccacattga gcgagtcgag ctcgacccgg atcggcagcc gtccctggag ctcggggatg
      481 aggtcggagg gcttcgacag gctgaacgca cccgaggcga tgaacagcac gtggtcggtc
      541 ttcaccgggc cgtacttggt cgccaccgtc gagcccccga cgagcggcag caggtcccgc
      601 tgcacaccct cgcgcgacac gtcggcgccg atcgtctcct gacggcgcgt caccttgtcg
      661 atctcgtcga tgaatacgat gccgttctgc tcggcgttgg cgtgcgcctg gatcttgagc
      721 tcgtcctcgt tgatgagctt gccggcttcc tcgtcgatga gcagcttcag ggcctcgcgc
      781 accttgacct tgcgggtgcg ggtgcggttg ccggagaggt tttggaacat tgactgcagc
      841 tgctgctgca tctcctccat cccgggcggc gccatgatct ccacgcccat cggcagcgcg
      901 cgcacctcga tctcgatctc gcggtcgtcg agctcgtgct cgcgcagcat cttgcggaat
      961 ttctgccgcg tctccgaatc gcgcgcgtgg ctcggctcgt cggtggagaa gcccatcatg
     1021 cgcggcttcg gcagcagcgt atcgagcacg cgctcctcgg ccgcatcgag cgcacgctcg
     1081 cgcaccttgg ccatctcctg ctcgcgggtg atcttgaccg cgacgtcagc cagctccttg
     1141 acgatcgagt cgacgtcgcg accgacatag ccgacctcgg tgaacttggt ggcctcgacc
     1201 ttcacgaatg gcgcgttggc gagcttggcg aggcgccgcg caatctcggt cttgccgacg
     1261 ccggtcgggc cgatcatgag gatgttcttg ggcgtgatct cctggcgcag cggctcctcg
     1321 acctgcatgc ggcgccagcg gttgcgcagc gcgatggcca cggcgcgctt ggccgcgtcc
     1381 tggccgatga tgtgcttgtc cagctcctgg acgatctggc gcggcgtgag tgcggatgcc
     1441 atgtggttac ttcgactccc tgcccttggc cgcgttcagc tcctcgatcg tgatgctgcg
     1501 attggtatag atgcagatgt cggcggcgat gtcgagggag cgctcgacga tggcgcgcgc
     1561 gggcagatcc gagccctcga gaagcgcccg cgcggcggcg agcgcgaact ggccacccga
     1621 gccgatcgcg gcgacgtcgt gatcgggctc gatcacgtcg ccgtttcccg agatgatgaa
     1681 gagcttctcg ggatcgccga ccaggagcag ggcttcgagc cgccgcaggt accggtccga
     1741 gcgccagtcc ttggcgagct cgatggcggc gcgcgtgaga ttgccgtact tctcgagctt
     1801 gccctcgaag cgctcgaaga gcgtaaaggc gtccgcagtg ccgccggcga agccggcgag
     1861 caccttgtcg ccgaagaggc ggcgcacctt gcgggcgttg cccttcatga ccgtgctgcc
     1921 aagggtcacc tggccgtcgc cgcctaaggc gacggcgccg ttgcggcgga caccgaggat
     1981 ggtcgtaccg tggggattga acactgtcgg gtcgctcata aggtcctagg gggtgctggt
     2041 cgcggggcgg gaatcgtcaa gccctgtcaa ggcttacggc gcgcccgcgg gtgtgcagcg
     2101 tcatacgtgc gggcgaggtg ctggaaatca aggtgagtat agatctgcgt ggtggagatg
     2161 tcggcgtggc cgagcagctc ctgcacgccg cgcagctccc cgcccgactc gagcaggtgt
     2221 gaggcgaagg aatgccggaa gagatgcggg tgcacgtgca cgccgaggcc ctggcgcttc
     2281 gcccaatagc tcacacgcgc ctggatggcg cggggaccga ggcgttggcc cgtgcgggcg
     2341 acgaagagcg cgggctcgtc catgggggcg agcgcagcgc gctccttgag ccaggcgcgc
     2401 agcgccgcga gcgccagcct gccgaccggt acgatgcgcg ccttgcgccc cttgccgagc
     2461 acccgcacgg tgcggtccct caagtcgagg tcggcgagat cgagtccgac gagctccgcg
     2521 agccgcaggc ccgaggagta gagcagctcc atcagcgcgc ggtcgcgcgc cgtgaggccc
     2581 ccgcccgccg ggatctgcag cagccgcccc atctggtcgg catcgagcgt gtgcggcagg
     2641 cggcgcgcgg ccttcggcgc gcgcacctcg agcgcgggat tcgcccgcac gcgcgcgccg
     2701 cgccgggagc gcttgccgct cgcggcctcg gccggcgcct cgcgcagcag gaactcgtag
     2761 aagctgcgca ccgccgacag ccgccgctgg atgctgcgcg ggctcagccc cccggcgtga
     2821 gagtgggcgg cgaagctgcg cacgtgctgg ctgtcgaggg cgctccactt ggcgagcccg
     2881 tgctggtcgc agaacttcac cagcgtgccg agatcacggg cgtaggccgc atcggtgtgt
     2941 gtcgacagcc gccgctcgga ggcgaggtag cggcggaagc gggcgagcca ctcgagcgcc
     3001 gccggactca cggcgcaggc cctgcgggaa gcccggtgac gctcaggctg cctcggtcgc
     3061 ggtcagcgca tcggcgatca ggtccgccat gcgggcgagg aattcggtgc tcatccccgg
     3121 gtgaaagcgg tcgcggtcga cgctgccgag cgccagcagc ccgatcgacc ccttggcgcc
     3181 gagcggcacc agtgccaccg agcccatctc cgggccctcg gggccgaaca ggaactcgcg
     3241 ctgcgagt
//
LOCUS       DATLKL010000082         2257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_170018, whole genome shotgun sequence.
ACCESSION   DATLKL010000082 DATLKL010000000
VERSION     DATLKL010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170018"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VM713_01085"
     CDS             complement(<1..408)
                     /locus_tag="VM713_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HVH82886.1"
                     /translation="MNLHEYQAKDLFRSSGIPVPAGKVAASPDEAAAAAAELGGGVWV
                     VKAQVHAGGRGKAGGVKLARDPEAVRAAASGMLGTRLVTKQTGPEGLPVDRVYVEAGS
                     GIARELYLSMTLNRERGRVALIASAAGGMEIEEV"
     gene            556..2187
                     /locus_tag="VM713_01090"
     CDS             556..2187
                     /locus_tag="VM713_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH82887.1"
                     /translation="MPASIAVVPALTPSELVWRVIGLLNLYRLLVPLLLLTMQALAGP
                     AFSLVPTHPQLFIGACIAYFTAALLLIIARRLEWSSLRIVALVNASVDAVAIAFILYG
                     AGGVASGLGILLVLPVFALAVLADRRDAFLIAAVAAIAVLAQQVFSGLTDATAATDYV
                     TAGIIGAALFATSLITWPVANRLRETDALVRRRDLDLANLAQLSQYIVQHLRESIIVI
                     DGQDRIRLINESAAEILGDNNAFPDALVGEASPRLLFLLESWRQGASGPGSGAPADPT
                     FVAADGARVIRPHFAALGSAIPGPVLIFLEDTSLLAQRVQQSKLAALGRLSASIAHEI
                     RNPVGAMSHAGQLLAESPSLTSDDRRLTEIIRANATRVSGIIDNVQRLSRREPARLER
                     LTLSAWTEEFQAEFCETMQVPRAQLEVSAAGDIEVSADPAQLRQILWNLCENALKHAV
                     RDDPSRAVEIRYGRMPANARPYLEVADRGEGVKPEIAERIFEPFFSSGRGSGLGLFLA
                     RELAQANGATLLYEPRAGGGSIFRMVFADRRRWEI"
BASE COUNT          340 a          811 c          741 g          365 t
ORIGIN      
        1 gacctcttcg atctccatgc cgccggccgc ggaggcgatc agcgcgacgc ggccgcgctc
       61 gcgattgagg gtcatcgaaa ggtagagctc gcgcgcgatg ccggagcctg cctcgacgta
      121 cacgcgatcg accggcagtc cctcggggcc cgtctgcttg gtgaccaggc gcgtccccag
      181 catcccggag gccgcggcgc gcaccgcctc cgggtcccgt gcgagcttca cgccgcccgc
      241 cttgccgcgg ccgccggcgt gcacctgcgc cttcaccacc cagacgccgc cacccagctc
      301 ggcggctgcc gcggcggctt cgtcggggct tgccgcaacc ttgccggccg gcaccggaat
      361 tccactgctg cggaagaggt ctttcgcctg gtattcgtgc aggttcatcg cgccgccagt
      421 cgaggtaaag catgcattct cgcgaaattg cccggcagtg gccagccatg cgatgcgggt
      481 cgcggccccc gccggagcga agatgctaga gtcgccggca cctgcaaggg gcccgcaaac
      541 gatcccgcac gcaaaatgcc ggccagtatc gcagtcgtcc ccgccctgac ccccagcgaa
      601 ctcgtctggc gggtcatcgg gctcctcaac ctctaccggc tgctggtgcc gctgctgctg
      661 ctcacgatgc aggcgctcgc gggccccgcg ttcagtctgg tgccgacgca cccgcagctg
      721 tttatcgggg cctgcatcgc ctacttcact gcagcgctgc tgctcatcat cgcgcggcgg
      781 cttgaatggt cgagcctgcg catcgtggcg ctcgtcaatg ccagcgtcga tgctgtcgcg
      841 atcgcgttca ttctgtacgg ggccggcggc gtcgccagtg gacttgggat tctcctggtg
      901 ctaccggtat tcgcgctcgc cgtgctggcc gaccgccgtg acgcatttct gatcgccgcg
      961 gtcgccgcga ttgcggtgct ggcacagcag gtattctccg gcctcaccga cgcgaccgcc
     1021 gcgacggatt acgtgaccgc cgggatcatc ggggcggcgc tgttcgcgac ttcgctcatc
     1081 acctggccag tcgcgaaccg gttgcgcgag accgacgccc tggtgcggcg cagggatctt
     1141 gacctcgcca acctcgcaca gctctcgcaa tacatcgtcc agcacctgcg cgaaagcatc
     1201 atcgtgatcg acggccagga ccgcattcgc ctcatcaacg agtcggcggc ggagatcctt
     1261 ggcgacaaca acgcctttcc cgacgcactg gtgggcgagg cctcgccgcg cctcctgttt
     1321 ctgctcgaat cgtggcgcca gggcgcctca ggccctgggt caggggcgcc cgcggatccg
     1381 accttcgtgg ccgcggacgg cgcacgggtg atccgcccgc attttgccgc gctcggctcg
     1441 gccatcccgg ggccggtgct catcttcctc gaggacacca gcctgctcgc ccagagggtg
     1501 cagcagtcga agctcgccgc gctcgggcgt ctgagcgcga gcatcgccca cgagatccgc
     1561 aatccggtcg gcgccatgag ccacgcgggc cagctgctcg ctgagtcgcc cagcctgacg
     1621 agcgatgacc gccggctcac cgagatcatc cgcgccaacg ccacgcgcgt gagcggcatc
     1681 atcgacaacg tacagcgcct atccaggcgt gagccggcgc ggctcgagcg gctgacgctg
     1741 tcggcatgga ccgaggagtt ccaggccgag ttctgcgaga ccatgcaggt cccgcgcgcg
     1801 cagctcgagg tgagcgccgc cggcgacatc gaggtcagcg cagatcccgc tcagctgcgg
     1861 cagatcctgt ggaatctgtg cgagaacgcg ctcaagcacg ccgtgcggga cgaccccagc
     1921 cgcgccgtcg agatacgata cggacgcatg cctgccaacg cgcgcccgta tctcgaggtc
     1981 gccgaccgcg gagagggagt aaaacccgag atcgccgagc gcatcttcga gccgttcttc
     2041 agctccgggc gcggctcggg cttaggtcta ttcctggcgc gcgagctcgc gcaggccaac
     2101 ggcgcaactc tcctgtacga gccgcgcgcg ggcgggggca gcatatttcg aatggtgttc
     2161 gcggaccggc ggcggtggga aatctgatga acgaggcagc aacaggggct cgcgcaacct
     2221 ccacgcagcc gctcgtgctc gtcgttgacg acgagcc
//
LOCUS       DATLKL010000083         3057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_57292, whole genome shotgun sequence.
ACCESSION   DATLKL010000083 DATLKL010000000
VERSION     DATLKL010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57292"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..>3057
                     /gene="mfd"
                     /locus_tag="VM713_01095"
     CDS             171..>3057
                     /gene="mfd"
                     /locus_tag="VM713_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HVH82888.1"
                     /translation="MTSLLNPPLPGAAAPRLEWRQLYGSAAALALAEAASSDARPYVV
                     IADAARELERLSAELRFFAGHALELLRLPDWEVLPYDLFSPHPDITSERLRTLYELPY
                     LKRGCLIVSADTLMQRLPPRDYVQGRAFELATGQALALEPFRQRLSDAGYASVSQVVS
                     PGEFAVRGSLLDVFPMGSGAPLRIDLFDEQIEAIRRFDPETQRSLESLDEVRLLPARE
                     VPLDAPAVRDFRRRYRTRFEGDPSKSALYRGVSEGLAPAGIEFYQPLFFDTLATLIDY
                     LPTDAVIVHDAALPQALARAWADIEARYEDRRHDLERPVLRPAELFLAPAELAAALER
                     FAAVELSAFKADTELGGASASLRNFPTAAPRELRLDMRAERPLAALDAFVREFHGRVL
                     IAADSPGRREMLAEMLRAHGHEVATVAGWEAFAGGAARLALAIAPDIEGLTLTEPPIA
                     LIAEAQLFGARARQERRRKRASDPEAILRDLQDLNPGAPVVHEEYGVGRYAGLQAMDI
                     AGQPGEFLVLEYLDGDRLYVPVHALHLVSRYTGAAPESAPLHKLGTDQWAKARRRAAE
                     QIRDVAAELLDLHARRRAQRGLSLTAAEPDYQAFVNGFPFEETADQAEAIRQVLADLA
                     AELPMDRIVCGDVGFGKTEVAMRAAFVAAAAGKQVAVLAPTTLLVQQHLGNFRDRFAD
                     WPLRIEALSRFGTSRETQATLAALEQGKVDIVIATHRLLHAQARFRDLGLLIIDEEQR
                     FGVRDKQRLQALRANVHVLTLTATPIPRTLNMALGGLRDLSLITTAPAARLAIKTFLI
                     EWHGPTLREAALRELRRGGQIYFVHNEVRSIAKIAAEVQTLVPEASVRIAHGQMRERE
                     LEQMMVDFYHRRFSLLVCTTIIESGIDVPSANTIMINRADHMGLAQLHQLRGRVGRSH
                     HRAYAYLIAPPRQALPRDAAKRLEAIESMEELGAGFVLANHDLEIRGA"
BASE COUNT          418 a         1140 c         1056 g          443 t
ORIGIN      
        1 gctcgacgcg cgcggcctcg acgtcggcgc gacagcgctg ccgccgctgc ggctgcgcgg
       61 ccgcgcgcgc ccaatcgaca tcttctgcgt gccgctcgcg gcgcgcctgc agctcgccgt
      121 ggggccggcc ggcacctgag caacgctctc gggtaagctg cccgagaccc atgacctcgc
      181 tcctcaatcc cccgctgccg ggcgcggccg cgccaaggct cgaatggcgc cagctctatg
      241 gcagcgccgc ggcgctcgcg ctcgcggagg cggccagcag cgatgcgcgg ccttacgtcg
      301 tgatcgccga cgccgcgcgc gagctcgagc gtttgagcgc ggagctgcgc ttcttcgccg
      361 gtcacgcgct cgagctgctg cgcctgccgg actgggaggt gctgccctac gacctgttct
      421 cgccgcatcc cgacatcacc tccgagcggc tgcggacgct ctacgagctg ccgtatctca
      481 agcgcggctg cctgatcgtg tcggccgaca ctctgatgca gcgcctgccg ccgcgcgact
      541 acgtgcaggg ccgcgccttc gagctcgcga ccgggcaggc gctcgccctc gagccgttcc
      601 ggcagcggct cagcgacgcc ggctacgcga gcgtcagtca ggtggtgagc cccggggagt
      661 tcgccgtacg cggctccctc ctcgacgtgt tccctatggg cagcggcgcg ccactgcgca
      721 tcgacctgtt cgatgagcag atcgaggcaa tccgccgctt cgatcccgag acgcagcgct
      781 cgctcgaatc gctcgacgag gtgcggctgc tgccggcgcg cgaggtgccg ctcgacgcgc
      841 cagccgtgcg cgacttccgc cgccgctatc ggacgcgctt cgagggggac ccgtcgaaga
      901 gcgccctcta tcgcggcgtg agcgagggcc tggctccggc cggcatcgag ttctatcagc
      961 cgctgttctt cgacacgctc gcaactctca tcgactacct gcccacggac gcggtgatcg
     1021 tgcacgacgc ggccctgccc caggccctgg cacgtgcctg ggccgacatc gaggcccgtt
     1081 acgaggaccg gcgccacgac ctcgagcgcc cggtgctcag gcccgcggag ctgttcctcg
     1141 cgcccgcgga gctcgcggcg gcgctcgagc gcttcgccgc ggtcgagctc tccgccttca
     1201 aggcggacac cgagctcggc ggcgcgagcg ccagcttacg caacttcccg accgccgcgc
     1261 cccgcgagtt gcgcctcgac atgcgcgccg agcggccgct tgccgccctc gatgctttcg
     1321 tgcgcgagtt ccacgggcgg gtactgatcg cggcggactc cccgggccgg cgtgagatgc
     1381 tcgccgagat gctgcgcgcg cacggtcacg aggtggcgac cgtggctggc tgggaagcct
     1441 ttgcgggcgg cgccgcgcgc ctcgccctgg ccatcgcgcc ggacattgag ggactcaccc
     1501 tgacggagcc gccgatcgcg ctgatcgccg aggcgcagct gttcggcgcg cgcgcccgcc
     1561 aggagcggcg gcgcaagcgc gcctccgacc cggaggcgat cctgcgcgat ctccaggacc
     1621 tcaatccggg cgcgccggtg gtgcacgagg agtacggcgt cggccgctac gccggcctgc
     1681 aggcgatgga catcgccggg cagccgggcg agttcctggt gctcgagtat ctcgatggcg
     1741 accgcctcta cgtgccggtg cacgcgctgc acctggtgag ccgctacacg ggcgccgccc
     1801 ccgagagcgc gccgctgcac aagctcggca ccgaccagtg ggccaaggcg cggcggcgcg
     1861 cggcggagca gatccgcgac gtggcggcgg agctgctcga tctgcacgcc cgccgcaggg
     1921 cacagcgcgg actctcgctg acggcggccg agcccgacta tcaggccttc gtgaacggct
     1981 tcccgttcga ggagaccgcg gatcaggccg aggccatccg ccaggtcctc gcggacctcg
     2041 ctgccgagct tcccatggac cgcatcgtgt gcggcgatgt cggcttcggc aagaccgaag
     2101 tagcgatgcg cgccgccttc gtcgccgcgg cggcgggcaa acaggtggcg gtgctcgctc
     2161 cgacgacgct cctggtgcag cagcatctcg gcaacttccg cgatcgcttc gcggactggc
     2221 cgctgcgcat cgaggcgctg tcgcgcttcg gcacctcgcg tgagacacag gcgacgctcg
     2281 ccgcgctcga gcagggcaag gtcgacatcg tgatcgccac gcaccggctg ctgcacgcgc
     2341 aagcgcgttt ccgcgacctc gggctgctca tcatcgacga ggagcagcgt ttcggcgtgc
     2401 gcgacaagca gcgcctgcag gcactgcgcg ccaacgtgca cgtgctcacg ctcaccgcga
     2461 ccccgatccc gcgcacgctc aatatggcgc tcgggggcct gcgcgatctg tcgctgatca
     2521 ccacggcgcc ggcggcgcgg ctcgcgatca agactttcct gatcgaatgg cacggcccga
     2581 cgctgcgcga ggcggcgctg cgcgagctgc gccgcggcgg ccagatctac ttcgtgcaca
     2641 acgaggtgcg ctcgatcgcc aagattgccg ccgaggtgca gacgctggtg cccgaggcga
     2701 gcgtgcgcat cgcccacggc cagatgcgtg agcgcgagct cgagcagatg atggtggatt
     2761 tctaccatcg gcgcttcagc ctgctggtgt gcaccaccat catcgagagc ggcatcgacg
     2821 tgccgagcgc caacaccatc atgatcaacc gcgccgacca catgggactc gcgcagttgc
     2881 accagctgcg cgggcgcgtc ggccgctccc accaccgcgc ctatgcctat ctcatcgcgc
     2941 cgccgcgcca ggcgctgccg cgcgatgccg ccaagcggct cgaagccatc gagtcgatgg
     3001 aggagctcgg cgcgggcttc gtgcttgcga accacgacct cgagatccgc ggcgcgg
//
LOCUS       DATLKL010000084         1509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_68638, whole genome shotgun sequence.
ACCESSION   DATLKL010000084 DATLKL010000000
VERSION     DATLKL010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68638"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..950
                     /locus_tag="VM713_01100"
     CDS             162..950
                     /locus_tag="VM713_01100"
                     /inference="COORDINATES: protein motif:HMM:NF025175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HVH82889.1"
                     /translation="MPFTLAHPAAILPLRRVRFLRTAPLVVGAVTPDVPYYLPLGPTG
                     HPLRLGLDTHSVVGSYTVDLALGMALLLGVLVLREPLTVLLPPRARWLCLEALEPFRR
                     RASVWLFAPLAILIGVWSHLLWDSFTHADGWAVRRIAALSDTVTIGWYTGEIFHILQY
                     LSSAIGLTILALWYVRLPVPPLAHAGHDSRRVHAGPALVLIAGAALLIGGVEALRYYA
                     HSEGAVYQTLDVLLTRGLAWFALLYLFAFAVVMLEHRSGAVRQR"
     gene            950..>1509
                     /locus_tag="VM713_01105"
     CDS             950..>1509
                     /locus_tag="VM713_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HVH82890.1"
                     /translation="MPLLMAADPGALQSTTTGWPWRAGNEFRLLDGGAEFFGRMLAAI
                     DAAQIHVLLEMYLVSSGQVAGRFIEALTRARARGVRCCVVFDGFGALGLSGGDRWWLE
                     EAGVELRIFNPLRWRNRLRNFQRDHRKLLVIDGQVAFVGGAGLTDEFAEPDAWRELMI
                     EITGPVVADWQRAFAGTWRRLGPELAL"
BASE COUNT          174 a          551 c          514 g          270 t
ORIGIN      
        1 ccacgccctc gggctgcatc tcgtcgaact cgacaccgcg cgcctcgcgg ccgggcggcg
       61 catcgatgtg accttccgcc acaccgcgag cgatgcctgg gtggggcggg actttgtgat
      121 ccgcgtaacc ccgcggcccg cgcccgccgg ataacacgcc gatgcccttc accctagctc
      181 acccggccgc gattctgccg ctgcgacgcg tgaggttcct gcgcaccgcg cccctcgtcg
      241 tcggcgccgt gacgccggac gtgccgtact atctgccgct cgggccgacc ggtcatccgc
      301 tgcggctcgg actcgacacg cacagcgtgg tcggctcgta cacggtcgac ctggcgctcg
      361 ggatggcgct gctgctcggc gtccttgtgc tgcgcgaacc gctcacggtg ctcctgccgc
      421 cgcgcgcccg ctggctgtgt ctcgaggcgc tcgagccttt caggcgccgg gccagcgtgt
      481 ggctgttcgc gccgctcgcg attctgatcg gcgtgtggtc gcatctgctg tgggactcgt
      541 tcacccatgc cgacggctgg gccgtgcggc gcattgccgc tctcagcgat accgtcacca
      601 tcggctggta caccggcgag atattccaca tcctgcagta cctgagctcc gccatcgggc
      661 tcacgatcct cgccctgtgg tatgtgcggc tgccggttcc cccactcgca cacgccggtc
      721 acgacagccg ccgggtgcac gccggcccgg cgctggtgct gatcgccggc gcggcactgc
      781 tcatcggcgg cgtcgaagcg ctgcgctact acgcccactc cgagggcgcc gtctaccaga
      841 ccctcgatgt gctcctcacc cgcggcctcg cctggttcgc gctgctgtat ctgtttgcct
      901 tcgccgtcgt gatgctcgag caccgctcgg gcgctgtgcg ccaacgctag tgccgttgct
      961 catggcggcg gatcccggcg cgctgcaatc caccaccacc ggctggccgt ggcgggccgg
     1021 aaacgagttc cgactgctcg acggcggcgc agagtttttc gggcgcatgc tcgcggccat
     1081 cgacgcggcc cagatccacg tcctgctcga gatgtacctg gtgagctccg gacaggtggc
     1141 cgggcggttc atcgaggcgc tgacgcgcgc gcgcgcccgg ggggtgcgct gctgcgtggt
     1201 cttcgacggc ttcggtgcgc tcgggctctc ggggggcgac cgctggtggc tcgaggaggc
     1261 gggtgtggag ctgcgcatct tcaacccgct gcgctggcgc aaccggctgc gcaacttcca
     1321 gcgcgaccat cgcaagctcc tcgtgatcga cggccaggtg gcgttcgtcg gcggggcggg
     1381 gcttaccgac gagtttgccg aacccgatgc gtggcgcgag ctgatgatcg agatcacagg
     1441 cccggtggtg gccgactggc aacgcgcctt tgccggcacc tggcggcgcc tcggtcccga
     1501 gctcgctct
//
LOCUS       DATLKL010000085         1654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_158756, whole genome shotgun sequence.
ACCESSION   DATLKL010000085 DATLKL010000000
VERSION     DATLKL010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158756"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..>1654)
                     /locus_tag="VM713_01110"
     CDS             complement(93..>1654)
                     /locus_tag="VM713_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HVH82891.1"
                     /translation="RLAVAAVAGGNAAGGALSGAGGARGAGLDAPAMRAMPLSEPEPS
                     VVVEETGTYERPRIESRGAAPPSQAAKQVTADDTISSETAINLDQGDPLAEADFHMAY
                     GLYDQAADLIRIAIGREPGRRDLKLKLLEVFFVWGNKEQFLQTAHELAQTREEAAPGE
                     WEKILIMGKQLAPEDALFSGGGAVGGAAAGGVDLNLEGGQSRVDFDLLGEPVPTEGQG
                     VDLDIGAAFGEGTVELTEATTGVTDRSLTALRDSGATGSTRQMTAKMRKQTADTEGPT
                     VEQPTLEQPAEDRTVRAKIEPRRRGGADQTAELAIDDLGLDLGQVDTQEQPMLGAGGE
                     GPTLVAGLDDRARRDLEDTANEAAQVKTSTTGQWQFDQEELESALTQGGAPPDSSHAQ
                     RLAALRGDTVDIDVHSAGQAVSASGVDVDLSSGGYPSNGGKLDLDVGTASVPDAAFAA
                     TQRLSSEDMALPDLEPVTMSEVGTKLDLARAYMDMGDPDGARNILEEVLTEGSAAQKQ
                     EAQRLMESLPG"
BASE COUNT          195 a          616 c          565 g          278 t
ORIGIN      
        1 cgggccagag tcgcgcctct tgcccgcggg cgtgaattca ccgtcagcgc cgctctgctg
       61 aaacggccgc aggccgtttc agccaatcga cctcatcccg gcagggactc catcagccgc
      121 tgcgcttcct gcttctgcgc cgccgagccc tcggtgagca cttcctcgag gatgttgcgt
      181 gcgccgtccg gatcgcccat gtccatgtag gcacgcgcga gatcgagttt ggtgccgacc
      241 tcgctcatcg tcacgggctc gagatccggc agcgccatgt cctcggacga gagccgctgc
      301 gtcgccgcga aggccgcatc cggcacgctg gccgtgccga cgtcgaggtc gagtttgccc
      361 ccgttgctcg ggtagccgcc cgagctgaga tcgacgtcga cgcccgaggc ggataccgcc
      421 tgaccggccg agtgcacatc gatgtcgacc gtatcgccgc gcagcgccgc cagacgctgg
      481 gcgtgcgagc tgtccggcgg cgcgccgccc tgcgtgagcg ccgactcgag ctcctcctgg
      541 tcgaactgcc actggcccgt ggtcgaggtc ttcacctgcg cggcctcgtt ggccgtgtct
      601 tcgaggtcgc ggcgcgcccg atcgtcgagt ccggccacca gggtcggccc ctcgccgccg
      661 gcgccgagca tcggctgctc ctgggtgtcc acctggccga ggtcgagacc gagatcgtcg
      721 atcgcgagct ccgcggtctg gtccgcgccg ccccgccgcc gcggctcaat cttcgcgcgc
      781 accgtgcggt cttcggccgg ctgctcgagc gtcggctgct cgaccgtcgg gccttcggtg
      841 tccgcggtct gcttgcgcat cttggcggtc atctgccgcg tgctgccggt ggccccgctg
      901 tccctgagcg cggtgaggct gcgatcggtg acgccggtcg tcgcctccgt gagctccacg
      961 gtgccctcgc cgaaggccgc accgatatcg agatcgacgc cctgcccctc ggtcggtacc
     1021 ggctcgccga ggagatcaaa gtcgacccgg ctctgcccgc cctcgagatt gaggtcgacc
     1081 ccgcccgccg ccgcgccacc gacggcgcca ccgccggaga acagcgcatc ctcgggcgcg
     1141 agctgcttgc ccatgatcag gatcttttcc cactcgccgg gcgcggcttc ctcgcgcgtc
     1201 tgcgcgagct cgtgggcggt ctgcaggaac tgctccttgt tgccccagac gaagaacacc
     1261 tcgaggagct tgagcttcag atcgcggcgc cccggctcgc ggccgatggc gatgcggatg
     1321 agatcggcgg cctggtcgta gagaccgtag gccatgtgga aatcggcctc ggcgagcggg
     1381 tcgccctggt cgagattgat cgcggtctcg ctgctgatgg tgtcatcggc agtgacctgc
     1441 ttcgccgcct gcgagggcgg cgcggcgccg cggctttcga tacgcggccg ctcgtacgtg
     1501 ccggtctcct cgacgacgac gctcggctcg ggctcggaga gcggcatggc gcgcatggct
     1561 ggcgcatcga gccctgcccc gcgcgcgccc ccggctccgc tgagtgcacc accggcggcg
     1621 ttccctccgg ccacggcggc cacggccagc cggc
//
LOCUS       DATLKL010000086         2625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_34839, whole genome shotgun sequence.
ACCESSION   DATLKL010000086 DATLKL010000000
VERSION     DATLKL010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34839"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /gene="pmbA"
                     /locus_tag="VM713_01115"
     CDS             <1..1235
                     /gene="pmbA"
                     /locus_tag="VM713_01115"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008268.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HVH82892.1"
                     /translation="SRGLSVSVRLGEVDTVEYQRDRGVAVTVYFGKRKGSASTADLGR
                     AAVFETVAKACAIARYTAEDPYAGLVEPEALAREIPDLDLDHAWELTPEQAIELARGC
                     EAAGLAVDARLRNSEGGAVGSQRHTGAYGNSLGFLAGYSATSHSLSCSLIAEEDGEMQ
                     RDHWYSVARDPADLEDAAEVGRIAGERAVARLGARRLTTRKAPVAFAPDMARGLIGHF
                     IGAIRGTSQYRKASFLLDAAGSQVFPAFVQMHERPHLPKGLASSPFDQEGAATHDREL
                     VRDGVLDGYVLGSYSARRLGLKTTGNAGGIHNLIVAASDGGLGAQAFLARLDQGLLVT
                     ELMGQGVNGVTGDYSRGASGFWVEHGAIQHAVHEVTIAGNLKRMYLDIAAVGADTDAR
                     GAIRTPSLLISEMTIAGE"
     gene            complement(1207..2565)
                     /locus_tag="VM713_01120"
     CDS             complement(1207..2565)
                     /locus_tag="VM713_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HVH82893.1"
                     /translation="MWQRIWRFVDRRLFGPASQRAAVLGAALRLLRYPYAVLRDLSRG
                     EINLRAMSLVYTTLLSLIPLLAFSFAILRFFGGHRDLVPFVYEFFRPVGEPAATDLTT
                     RVVQFASHASNGVVGSVGFALLAWTLIGTIKKVEDSFNFLWHVEQPRSFARRLAEYSA
                     LLVAGPVLLVGFVGLTQHALASAPVQEVVRMRLPVLQWLRAAGIALAPYAMVSAFFTA
                     LYMLVPNTRVQWRAALTGAIVGGVLWAAVGKVFTSLVVYSTRLTIVYAGFAFVVAGLV
                     WTYFGWLILLAGAQLSFYVQNPAYLRLGLQQLVLSGLELEELALKLMYFVGRAHVAGG
                     ARWTVKRVAAELGLPGVAVARMAALLEGAGLLVVTDSGQLIPGRDIGRIPVEEILDLA
                     RSERSGHVAPRPLDIPPVDRVLASLEEARRRCCGELTLRELVEEAPRPALQLTHRQSS
                     FR"
BASE COUNT          436 a          920 c          931 g          338 t
ORIGIN      
        1 tgagccgcgg gctgtcggtg agcgtgcggc tcggcgaggt cgacaccgtc gagtaccagc
       61 gcgaccgcgg cgtcgcggtg acggtctatt tcggcaagcg caagggctcg gccagcaccg
      121 ccgatctcgg gcgggcggcc gtcttcgaga cggtagccaa ggcgtgcgcg atcgcacgtt
      181 acacggccga ggacccttac gccgggctcg tcgagcccga ggcgctcgcg cgcgagatcc
      241 ccgacctcga tctcgaccac gcctgggagc tgaccccgga gcaggcgatc gagctggcgc
      301 gcggctgcga ggcagccggt ctcgccgtcg acgcgcggct gcgcaactcc gagggtggcg
      361 cggtcggcag ccagcgccat acgggcgcct acggcaactc cctcggattt ctcgccggtt
      421 actccgccac cagccactcg ctcagctgct cgctgatcgc cgaggaagac ggggagatgc
      481 agcgcgacca ctggtacagc gtggcgcgcg atccggccga tctcgaggat gccgcggagg
      541 tcggccgcat cgccggcgag cgcgccgtcg cgcgcctcgg cgcgcggcga ctcactacgc
      601 gcaaggctcc cgtcgccttc gcgcccgaca tggcacgcgg cctcatcggg catttcatcg
      661 gtgcgatccg cggcaccagc cagtatcgca aggcgtcgtt ccttctcgat gcggccggct
      721 cgcaggtatt tcccgccttc gtgcagatgc acgagcggcc gcatctgccg aaggggctcg
      781 ccagcagccc cttcgaccag gagggcgccg cgacccacga tcgcgagctg gtgcgcgacg
      841 gcgtgctcga cggctacgtg ctcggcagct actcggcgcg gcggctcggg ctgaagacca
      901 cgggcaacgc cggcgggatt cacaacctga tcgtggcggc gagcgacggc gggctcggcg
      961 cgcaggcgtt tctcgcccgg ctcgaccaag gattgctggt gaccgagctc atggggcagg
     1021 gcgtgaacgg cgtcaccggc gactactcgc gcggcgcgag cggcttctgg gtcgagcatg
     1081 gcgcgattca gcacgcggtg cacgaggtga ccattgccgg gaacctgaag cgcatgtacc
     1141 tcgacatcgc cgccgtcggc gcggacaccg acgcgcgcgg cgccatccgc accccctcgc
     1201 tcctgatcag cgaaatgacg attgccggtg agtgagctgc agcgccgggc gcggcgcctc
     1261 ctccaccagc tcacgcagcg tgagctcgcc gcagcacctg cggcgcgctt cctcgaggct
     1321 cgcgagcacc cggtcgacgg gtgggatgtc gagggggcgc ggtgccacgt ggccgctgcg
     1381 ctcgctgcgc gcgaggtcga ggatctcctc cacggggatg cggccgatgt cgcggccggg
     1441 gatgagctgg cctgaatcgg tcacgacgag gaggccggcc ccctcgagca gcgccgccat
     1501 ccgtgccacc gcaaccccgg gaaggccaag ctcggcggcg acgcgcttga ccgtccagcg
     1561 cgcgccgccg gccacgtgcg cccggcccac gaagtacatc agcttcagcg ccagctcctc
     1621 gagctcgagg cccgagagca ccagctgctg gagcccgagg cgcagatacg ccgggttctg
     1681 cacgtagaag gagagctgtg ccccggcgag caggatcagc cagccgaagt aggtccagac
     1741 gagcccggcg accacgaagg cgaagcccgc ataaacgatg gtgaggcggg tcgagtagac
     1801 gacgagagac gtgaatacct tgccgaccgc tgcccagagc acgccgccga cgatggcgcc
     1861 ggtgagcgcc gcgcgccact gcacccgggt gttgggcacc agcatgtaga gcgcggtgaa
     1921 gaacgcgctc accatggcat aaggggcgag ggcgatgccg gcggcgcgca gccattgcag
     1981 caccggcagg cgcatccgca ccacctcctg caccggggcg ctcgcgagcg catgctgggt
     2041 gagtcccacg aagcccacca gcagcaccgg tcccgccacc agcagcgctg agtactccgc
     2101 gaggcgccgc gcgaagctgc gcggctgctc gacgtgccac aggaagttga agctgtcctc
     2161 gaccttcttg atggtgccga tgagcgtcca ggcgagaagc gcaaagccca ccgagccgac
     2221 caccccgttg ctcgcgtggc tggcgaactg caccacacgc gtagtgaggt cggtcgccgc
     2281 cggctcgccg accgggcgga agaactcgta cacgaagggc accaggtcgc ggtgcccgcc
     2341 gaagaaccgg aggatcgcaa agctgaaggc gagcagcggt atcagcgaca gcagtgtcgt
     2401 gtacaccagg ctcatcgcgc gcaggttgat ctcgccgcgc gacaggtcgc gcagcaccgc
     2461 gtaggggtaa cgcagcagcc gcagcgcggc tcccaggact gccgcgcgct gggaggccgg
     2521 cccgaacagc cgccggtcga cgaaccgcca gatgcgctgc cacatggctt aaggatacag
     2581 tgccgccggt gcaagacgga tatccgcagc ggatcgtgtg cctca
//
LOCUS       DATLKL010000087         4373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_57332, whole genome shotgun sequence.
ACCESSION   DATLKL010000087 DATLKL010000000
VERSION     DATLKL010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57332"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VM713_01125"
     CDS             complement(<1..557)
                     /locus_tag="VM713_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HVH82894.1"
                     /translation="MFDQLTARLSATLESLRGRGRITEDNIAQALRDTRIALLEADVA
                     LPVVKSFIEAVKAKALGAEVASSLTPGQAFIGILHEELVALMGGGARDFSLRAQPPVV
                     VLLAGLQGAGKTTTGAKLARWLIESGRKRVLLASTDVRRPAAMLQLERLAAQVHAEYF
                     PPEAAAAPEDIARAALTRARNGVFDA"
     gene            633..1922
                     /locus_tag="VM713_01130"
     CDS             633..1922
                     /locus_tag="VM713_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HVH82895.1"
                     /translation="MNVASTPWPLLALLALLIVAAFSSGAEAAMLSVNRYRIRHRAQA
                     GQLSARLLERLLAKPDEWLGANLVILAPASVFASAIATILAQRSGYAYAVPVTEVLLT
                     IVVIVFCELAPKIYAASNPEGVALHAAGIYRVLVLVARPALWLTNRMAYGFLRIFGVG
                     RAAQTSHALSAEELRTVVTEAAPVIPARHRQMLLSILDLGRITVNDIMIPRQEIAGID
                     VSESWEDILERLRQTPHTRLPVYEGELDNLIGLLHMKRAARELVRGTFTREHLIELAR
                     GRESYFIPEGTPLNVQLAHFQRNRRRFAFVVNEYGDIDGLVTLEDILEEIVGEFTTDP
                     ATITHKDVHTERPGVFIVNASATIRALNRALGWQLPTGGPKTVNGLLLEQLETIPETG
                     AMVRVGSYEFEVLQIADNAIRTVRVRTPVAEKATAAS"
     gene            complement(1919..3265)
                     /gene="radA"
                     /locus_tag="VM713_01135"
     CDS             complement(1919..3265)
                     /gene="radA"
                     /locus_tag="VM713_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HVH82896.1"
                     /translation="MARLNPVFVCASCGGETLKWQGQCPLCGEWNSLEERRAPRARAA
                     AAATAVSLAPGAALGSGGERLPSGLAELDRVLGGGIVPGSVILLGGDPGIGKSTLLLH
                     VAAHVAATRPVIYASGEESVAQLGLRAQRLGVAAEALGVVTETDCQAVLALAATRQAA
                     LLVIDSIQTLQSGAVPAGAGAVTQLRECTAELVRFAKSGGAAIIIVGHVTKEGAIAGP
                     KLLEHLVDTVLYFESETGSRYRIVRATKNRFGAVNELGFFAMTGTGLKEVKNPAAIFL
                     ARGSQPAAGSIVTVTRDGGRPLLIELQALVDRMRFGAPRRIAQGLDANRLAMLLAVLN
                     RHAGVALQEHDVFVNVVGGIQISETAWDLPVAIALASTLRNTALSAQLVAFGELGLTG
                     EVRPVPYGDERLREAQAQGFSVALIPRDNAPRKSPAGLTVHAVSTVAEALAAAFAR"
     gene            3446..4270
                     /locus_tag="VM713_01140"
     CDS             3446..4270
                     /locus_tag="VM713_01140"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HVH82897.1"
                     /translation="MSLRSKLRFVGATDTGKVREHNEDTIAVDADIGLLVLADGMGGY
                     NAGEVASGIAVKTIVNLVREHVEREDLAIADRDTGLSRPNIILRDAINRANKIIYQTA
                     RTQPQCEGMGTTVVAALFFDNKVTVAHVGDSRLYRQRGDRLEQVTMDHSLLQELVDRG
                     FYSAEEAQRASNKNYVTRALGVEPQVDVEIQEYPVARGEYFILCSDGLSDMVEDEDIH
                     LTINTFSANLDTVAKQLIQLANDNGGRDNISVVMAHIMDAFPARTKIFDKLLSWFG"
BASE COUNT          734 a         1520 c         1446 g          673 t
ORIGIN      
        1 gcatcgaaca ccccgttgcg cgcgcgtgtc agcgcagcgc gcgcgatgtc ctccggcgcc
       61 gccgcggcct cgggcggaaa gtactccgcg tgcacctgcg cggcgaggcg ctcgagctgc
      121 agcatcgcgg ccggccgccg cacgtcggtg ctggcgagga gtacgcgctt gcgcccgctc
      181 tcgatgagcc agcgcgccag cttcgcaccc gtcgtcgtct tgcccgcccc ctggagcccg
      241 gcgagcagca ccacgaccgg cggttgcgcg cgcaagctga agtcgcgagc gcctcccccc
      301 atgagcgcga cgagctcctc gtgcagaatg ccgatgaagg cctgccccgg agtgaggctc
      361 gaggccacct cggcgccgag cgccttggcc ttgacggcct cgatgaagga cttcaccacc
      421 ggcagcgcca cgtccgcctc gaggagcgcg atgcgcgtgt cacgcaatgc ctgcgcgatg
      481 ttgtcctcgg tgatgcggcc gcggccacgc aggctctcga gcgtcgccga caaccgcgcg
      541 gtgagctggt cgaacatgca gtcaggatcc ccgattccag cgcgctatgc tagtcgctcg
      601 accgtcgccg ccgcaccgac cggagagccg ccttgaacgt cgcttcgacc ccatggcccc
      661 tgctggcgct gcttgcgctg ctcatcgtcg ccgccttctc gtccggggcc gaggcggcga
      721 tgctctcggt caaccgctat cggatccgcc accgcgccca ggccgggcag ctgagtgccc
      781 ggctcctcga gcggctgctc gcgaagcccg acgagtggct cggcgcgaac ctggtgatcc
      841 tcgccccggc gagcgtgttc gcctccgcta tcgccaccat cctcgcgcag cgcagcggct
      901 atgcctatgc ggtgccggtc acggaagtgc tgctcaccat cgtggtgatc gtgttctgcg
      961 agctcgcacc gaagatctat gccgcctcca atcccgaagg cgtcgcgctg cacgccgccg
     1021 gcatctatcg cgtgctggtg ctggtggcgc gcccggcgct gtggctcacc aaccgcatgg
     1081 cgtacggctt cctgcgcatc ttcggcgtag ggcgcgccgc acagacgagc cacgcgctct
     1141 ccgccgagga gctgcgcacg gtcgtgaccg aggccgctcc ggtgatcccg gcgcgccacc
     1201 ggcagatgct gctctcgatc ctcgatctgg gacggatcac cgtgaacgac atcatgatcc
     1261 cgcgccagga gatcgccggc atcgacgtga gcgagagctg ggaagacatc ctcgagcggc
     1321 tcaggcagac gccgcacacg cggctgccgg tctacgaggg cgagctcgac aatctgatcg
     1381 ggctgctgca catgaagcgc gcggcgcgcg agctggtgcg cggcaccttc acgcgcgagc
     1441 atctcatcga gctcgcgcgc ggccgcgagt cgtacttcat cccggaggga accccgctca
     1501 acgtgcagct cgcgcatttt cagcgcaacc gccggcgctt cgccttcgtc gtgaacgagt
     1561 atggcgacat cgacggactg gtgacgctcg aggacatcct cgaggagatc gtcggcgaat
     1621 tcactaccga tcccgccacc atcacccaca aggacgtcca caccgagcgg ccgggcgtct
     1681 tcatcgtgaa cgccagcgcc accatccgcg cgctgaaccg cgcgctcggc tggcagctgc
     1741 cgaccggggg accgaagacg gtgaatggcc tgctgctcga gcagctcgag accattcccg
     1801 agacgggcgc gatggtgcgg gtcggcagct acgagttcga ggtgctgcag atcgcggaca
     1861 atgccattcg caccgtgcgg gtgcgcaccc ccgtggccga gaaggccacg gcagcctctt
     1921 aacgggcgaa ggcggccgcg agcgcctcgg caaccgtgct gaccgcgtgg acggtgaggc
     1981 ccgcggggga cttgcgcggc gcgttgtcgc gcggaatcag cgcgacgctg aagccctgcg
     2041 cctgcgcctc gcgcagccgc tcatcaccat agggcaccgg acgcacttcg ccggtgagcc
     2101 cgagctcgcc gaacgccacg agctgcgccg agagcgcggt gttcctgaga gtggacgcga
     2161 gtgcgatcgc aaccggcagg tcccaggcgg tctcgctgat ctgaatgccg cccacgacgt
     2221 tgacgaacac gtcgtgttcc tgcagcgcca ctcccgcgtg gcggttgagc accgcgagca
     2281 gcatcgccag gcggttggca tccagcccct gcgcgatgcg gcgcggggcg ccgaagcgca
     2341 tacggtcgac cagcgcctgc agctcgatga gcagcggcct gccgccgtcg cgtgtcaccg
     2401 tgacgatgct gccggcggcc ggctgtgagc cgcgcgcgag aaagatcgcc gccgggttct
     2461 ttacttcctt caggcccgtg ccggtcatgg cgaagaaccc gagctcgttg accgcgccga
     2521 aacggttctt ggtggcgcgc acgatgcggt agcggctgcc ggtctcgctc tcgaaataca
     2581 acaccgtgtc gaccaggtgc tcgagaagct tcggccccgc gatcgcaccc tccttggtca
     2641 cgtgaccgac gatgatgatc gccgcgccgc ccgacttggc gaagcgcacc agctccgcgg
     2701 tgcactcgcg cagctgcgtc accgccccgg cgccggccgg cacggcaccg gactgcaggg
     2761 tctggatcga gtcgatgacg agcagcgccg cctgccgcgt tgccgcaaga gccaggaccg
     2821 cctggcagtc cgtctcggtc acgaccccga gagcctcggc ggccacaccc aagcgctggg
     2881 cgcgcagccc gagctgtgcc actgactcct cgccgctcgc gtagatcacg ggccgcgtgg
     2941 cggccacgtg ggccgccacg tgcagcagca gcgtcgactt gccaatgccc ggatctcctc
     3001 ccaggagaat caccgagccc gggacgatcc cgcccccgag gacccgatcg agctccgcca
     3061 gcccgctcgg cagccgctcg ccgccgctgc cgagggcggc cccgggcgcg aggctcaccg
     3121 cggtggcggc cgctgcggcg cgggcgcgcg gtgcgcgccg ctcctcgagc gaattccact
     3181 cgccgcacaa aggacactgc ccctgccact tgagggtttc gcccccgcag gaggcacaca
     3241 cgaacaccgg attcaggcga gccatcgatg cgccgtcggt cgactttcgg ataggatgcg
     3301 cgccggggag atcaccaaaa gcggtctttt gtgaccaatc actcggacac ccctgcgggc
     3361 gatgctagct tatgcaagct cgaatcgggg acgggaaagt gatgcgtatc acatttggtg
     3421 ggccgccggc tggtcagggc acagcatgag cttgcgaagc aagctgcgct tcgtcggtgc
     3481 caccgatacc ggcaaggtcc gggagcacaa cgaggatacc atcgcggtcg acgccgacat
     3541 cggcctgctg gtgctcgcag atggcatggg cggttacaac gccggagaag tggcgagcgg
     3601 catcgcagtg aagaccatcg tcaatctggt gcgcgagcac gtcgagcgcg aggacctcgc
     3661 gatcgcggac cgtgacacgg gcttgtcccg gccgaacatc atcctgcgcg acgccatcaa
     3721 ccgcgccaat aagatcatct accagactgc gcgcactcag ccgcaatgcg aaggcatggg
     3781 cacgacggtg gtcgcggcgc tgttcttcga caacaaggtc accgtcgccc acgtcggcga
     3841 ctcgcgcctg tatcgccagc gcggcgaccg gctcgagcag gtcaccatgg accattcgct
     3901 gctgcaggag ctggtcgacc gcggctttta ctccgccgag gaggcgcagc gggcgtccaa
     3961 caagaactac gtgacccggg cgctgggggt cgagccccag gtcgacgtcg agatccagga
     4021 ataccccgtg gcccggggcg agtacttcat cctctgctcg gacggacttt ccgatatggt
     4081 agaggatgaa gatatccatt taaccataaa cacctttagt gctaatctgg atacggtcgc
     4141 caagcagttg attcagctgg ctaatgataa tggaggccgt gacaacatct cggtggtgat
     4201 ggcgcacatc atggatgcgt ttccggctcg cacgaagatt ttcgacaaac ttctgagttg
     4261 gttcggctga cacgacgcga taggggctag caaatggcaa ggttggtctt gagcctggac
     4321 ggccaggtga tggcggaata caacatgaac aaggagcggt acaccatcgg ccg
//
LOCUS       DATLKL010000088         2629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_147514, whole genome shotgun sequence.
ACCESSION   DATLKL010000088 DATLKL010000000
VERSION     DATLKL010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147514"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VM713_01145"
     CDS             <1..622
                     /locus_tag="VM713_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVH82898.1"
                     /translation="VPLPAVGQAAVARPIQAPGAAATPAPAPAISAPGSFQEDTAEEV
                     GGANQPPASPPETKVAGFAPAAGVAGAARSAAAGTGLFLWPLQGKILSGYGPKEGGLF
                     NDGINIAAKRGQIVIAAGAGTVVYAGNEIRGFGNLVLIKHPSGWVTAYAHNDRLLVRR
                     GERVKRGQPIAKVGTSGGVSSPQLHFEVRKGSHAVDPLKYLGPLPT"
     gene            complement(632..1120)
                     /locus_tag="VM713_01150"
     CDS             complement(632..1120)
                     /locus_tag="VM713_01150"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH82899.1"
                     /translation="MPSLPRLLARLRQATRRFILFCDDLSFDGQDTTYKSLKAVLEGG
                     IEGRPDNVVFYATSNRRHLMPRDMIENERSTAINPSEAVEEKVSLSDRFGLWLGFHHC
                     DQDTYLEMVEGYAARHGLKLPRDQLHREATEWSITRGARSGRVAWQFIQDLAGRLGKQ
                     LD"
     gene            complement(1129..1488)
                     /locus_tag="VM713_01155"
                     /pseudo
     CDS             complement(1129..1488)
                     /locus_tag="VM713_01155"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
     gene            1687..2067
                     /gene="yajC"
                     /locus_tag="VM713_01160"
     CDS             1687..2067
                     /gene="yajC"
                     /locus_tag="VM713_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HVH82900.1"
                     /translation="MLISTAFAQAAGSQGGEFNMMSILPLVLIFVVFYFLLIRPQQKK
                     MKDHKAMIGSLRRGDRIVTGGGIVGTVVKSQGDEDCVVEIADGVRVRVVRSTIQSVLS
                     RTEPAGEKEKNSGDEARKVDGGKK"
     gene            2113..>2629
                     /locus_tag="VM713_01165"
     CDS             2113..>2629
                     /locus_tag="VM713_01165"
                     /inference="COORDINATES: protein motif:HMM:NF019187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82901.1"
                     /translation="MVHLAKWKVVLILGLSLLGIIFAAPNLLSREAAESLPHWLPHQQ
                     ISLGLDLQGGSHLLFEVDTRSVINEELNALVDAARNTLRKERIFYTGLGISGNSVVFQ
                     LRDPADIDRVKGLIREFSSAVSGTGAGLGSIVGGLTSGQSLVIRAADQGAVTLELSDQ
                     AIKDRETRAVEQ"
BASE COUNT          460 a          852 c          893 g          424 t
ORIGIN      
        1 cgttccgctc ccggctgtcg gtcaagctgc tgtggcgcgg ccgattcagg cgcctggcgc
       61 ggctgcgacg cccgcccccg ctccggcgat ctcagcacca ggctccttcc aggaggatac
      121 ggccgaggaa gtcggaggcg cgaaccagcc gccggcgtcg cctccggaga ccaaagtcgc
      181 aggcttcgca cccgccgccg gcgtggcggg cgcagcacgt tccgccgcgg ccgggaccgg
      241 cctctttctg tggccgctcc agggcaagat cctctccggc tatggtccga aggagggcgg
      301 gctgttcaac gacggcataa acatcgcggc caagcgcggg cagattgtga tcgcggccgg
      361 cgctggcacc gtggtttacg cgggcaacga gatccgcggc ttcggcaacc tcgtgctgat
      421 caagcacccg agcggctggg tgactgccta cgcccacaat gaccggctgc tggtcaggcg
      481 cggcgagcgg gtcaagcgcg gccagcccat cgccaaggtg ggaacttcgg gaggtgtttc
      541 gagcccgcag cttcatttcg aagtccgcaa gggctcccac gcggtggacc ccctcaaata
      601 cctcggcccg ctcccaacct gagcggccgg cctagtccag ctgcttgccg agccgcccgg
      661 ccaaatcctg gatgaactgc caggccacgc gccccgaacg cgcaccgcgg gtgatcgacc
      721 actcggtggc ttcccgatgg agctggtcac gcggcagctt gaggccgtgg cgcgcggcat
      781 agccctcgac catctccaga tacgtatcct ggtcgcagtg atgaaatccg agccacaggc
      841 cgaagcggtc ggagagcgaa accttctcct cgaccgcctc cgagggattg atggctgtcg
      901 agcgctcgtt ctcgatcatg tcgcgcggca tcagatggcg gcggttcgag gtggcgtaga
      961 acaccacatt gtccggccgg ccctcgatgc caccttccag cacggccttg agcgacttgt
     1021 aggtggtgtc ctggccgtcg aaggagaggt cgtcgcagaa gaggatgaag cgccgcgtgg
     1081 cctggcgcag gcgcgcaagc agccggggaa gggaagggat gtcctcgcgg tgatctcgac
     1141 cagcgccaga gcacgcgggc gttcagcgtt gaggtgggca tggatcgcct tgaccagcga
     1201 gctcttgccc atgccgcgcg cgccccacag caaggcgttg ttggccggca gcccctcggc
     1261 gaagcgccgc gtattggcca acagaacttc gcgctgcgat acgatcccct tgagaagctc
     1321 gaggtcgacc cgattcactt cgcgcaccgg ctcgagccat tcgccttcgg cgtgccagat
     1381 gaaggcgtcg gccgccgcga agtcgccccc gctcggcggc gccggggcca ggcgatcgag
     1441 cgccgccgcg atacggacca ggagggcgtc gaagtcgggt ccggccatgg tttctcccgc
     1501 gccggtcaga gcggaccgca acctaggcgg agccgggccc gcggtcaaat ggcgcgagcc
     1561 gcggcgcggt gacgaccgcg ccaacgccgc gggcgaaggc gtttgctctc ggccgcccga
     1621 gcgggtaagt ttcgccccgc tcaaccggcc attaccctta tttcgagcca aggggagaca
     1681 aaaatcatgt tgatttccac ggcctttgcc caggctgccg gcagccaagg cggcgagttc
     1741 aacatgatgt cgatcctgcc gctcgtcctc atcttcgtgg tgttctactt cctcttgatt
     1801 cggccgcagc agaagaagat gaaggaccat aaggcgatga tcgggtcgct tcgacgcggc
     1861 gaccgcatcg tgacaggcgg cgggatcgtg gggaccgtcg tcaagtctca gggcgacgag
     1921 gattgcgtgg tggagatcgc ggacggcgtg cgggtgcgcg tcgtgcgcag caccattcaa
     1981 tcggtcctct cgcgcacgga gccggcgggc gagaaggaga agaattcggg cgacgaggca
     2041 aggaaggtcg acggcggcaa aaagtgagat ggaacgccat atcgagaaag ggcgccggga
     2101 tcggttgagg ccatggtcca cctcgcgaag tggaaggtcg ttcttatcct ggggctaagc
     2161 ttgctcggaa tcattttcgc tgcgcccaat ttattaagcc gcgaggctgc ggagagcctc
     2221 ccgcactggc tcccccatca gcagatcagc ctcggcctcg atctccaggg tgggtcgcac
     2281 ctgctcttcg aggtcgacac ccggtcggtc atcaacgagg agctcaacgc gctcgtggac
     2341 gccgcgcgaa atacgctgcg caaggagcgc atcttctaca cgggactcgg catctccgga
     2401 aacagcgtgg tcttccagct tcgcgatcct gctgacatcg accgggtgaa aggcttgatc
     2461 cgcgagttct caagcgcggt cagcggcacg ggggctggac tcggcagcat cgtgggcggg
     2521 cttacctcgg gacaaagcct cgtgatccga gcggccgacc aaggcgccgt cacgctggag
     2581 ctctcggacc aggccatcaa ggatcgcgag accagggccg tggagcagt
//
LOCUS       DATLKL010000089         1654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_215076, whole genome shotgun sequence.
ACCESSION   DATLKL010000089 DATLKL010000000
VERSION     DATLKL010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215076"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VM713_01170"
     CDS             <1..512
                     /locus_tag="VM713_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVH82902.1"
                     /translation="LWLLVIGANILASGVANALAAQAAVSRVLYAMARDGRLPAVLAR
                     IHPRYQTPHVSILLVAGISIAVGSIFANRLDDLTRIVNFGALTGFLLLHVAVINYYVI
                     RGGSRAWVRHLLLPLAGLAVLGYVLYEMDTAAKIMGAVWIAAGMVYFAALTLLLKKPA
                     AIDLSGGIR"
     gene            509..>1654
                     /locus_tag="VM713_01175"
     CDS             509..>1654
                     /locus_tag="VM713_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82903.1"
                     /translation="MRVFLGMCLALATAANSAGARASTTEIAITHVNVIDASGTAPQP
                     DMTVIIRDQRIVELGRSAAVHPSTDARITNAHGKFVIPGLWDMHVHTVFGDWLPRNEK
                     VVLPLFVANGVTGVRDMGGDLDVLKEWRAKIAAGQLLGPRMYIAGPMLDGPVPRFPSS
                     APVANASDGRKVVDDLKARGVDFIKIQSLIPRDGYFAAAEEARKVGLTFVGHVPDAVR
                     ASEASAAGQKSIEHFTGVFEGCSTIEDQLVSGHPRGPGPNVDTYDAARAKALIALMAK
                     NRTWQVPTLVWERGQWLVDDIDKTHDPLTKYAPASWKDRTWPMFVHDIMQYMDTDPLP
                     VRKRFFQMELEMTLAMFRAGVPFMAGTDTAAGVHVFPGFSLHEELALF"
BASE COUNT          278 a          535 c          544 g          297 t
ORIGIN      
        1 gactgtggct gctcgtaatc ggcgcgaaca ttcttgcctc gggcgtcgcc aacgcgctgg
       61 cggcgcaggc cgcggtgtcg cgcgtcctct atgccatggc ccgcgacggc aggctcccgg
      121 cggtgctcgc gcggattcat ccgcgctacc agacacccca cgtgagcata ctgctcgtgg
      181 ccggcatatc gatcgcggtt gggagcatct tcgccaatcg cctggatgac ctcacccgca
      241 tcgtcaattt cggggcgctc acgggattcc tgctgctgca cgtggccgtg atcaactact
      301 acgtgatccg cggcggcagc cgggcctggg tgcgccacct gctactgccg ctcgccggcc
      361 tcgccgtgct cggctacgtt ctttacgaga tggataccgc cgccaagatc atgggtgctg
      421 tgtggatcgc ggcgggaatg gtgtatttcg cggcgctcac gttgctgttg aagaagccgg
      481 ccgccatcga tttgtcgggg ggtatccgat gagagtgttt ctcggcatgt gcttggcgct
      541 cgccaccgca gccaacagcg caggcgcgcg tgcctcgacc acggaaatcg cgatcacgca
      601 cgtcaacgtg atcgacgcga gcggaaccgc gccgcagccg gacatgacgg tgatcatacg
      661 ggatcaacgc atcgtcgagc tcggcaggag cgccgcggtg cacccgtcca ccgacgccag
      721 gatcacgaat gcgcacggca agtttgtgat tcccggcctc tgggacatgc acgtgcacac
      781 cgtcttcggc gactggctgc cgcgcaacga gaaggtcgtg ctgccgctgt tcgtcgcaaa
      841 tggcgtcacg ggcgtgcgag acatgggcgg cgacctcgac gtgctcaagg agtggcgtgc
      901 aaagatcgcg gccgggcagc tgctcgggcc gcggatgtac atcgccgggc ccatgctcga
      961 cggccccgtg ccgcgcttcc cgagctctgc accggtcgca aatgccagcg acggccgcaa
     1021 ggtggtggac gatctcaagg cccgcggcgt cgacttcatc aaaatacagt cactcattcc
     1081 gcgcgacgga tacttcgccg cggccgagga ggccaggaag gtgggcctga ccttcgtcgg
     1141 acacgtgccg gacgcggtgc gtgccagcga ggcttccgcc gccggccaga agagcatcga
     1201 gcacttcacc ggcgtcttcg aggggtgctc gaccatcgag gaccagctcg tcagtggtca
     1261 tcccaggggt ccgggtccca acgtcgacac ttacgatgcc gcccgggcta aggcgttgat
     1321 cgcactcatg gcaaagaatc ggacctggca ggtgccgact ttggtctggg agcgtggtca
     1381 gtggctggtt gacgacatcg acaaaactca cgacccgctg acgaagtacg cgcctgcgtc
     1441 ctggaaagat cgcacctggc cgatgttcgt gcacgacatc atgcagtaca tggataccga
     1501 tccgctgccc gtccgcaagc gcttcttcca gatggagctc gagatgacgc tcgctatgtt
     1561 ccgcgctggc gtgccgttca tggcgggcac cgacacggcg gcgggcgtgc acgtcttccc
     1621 ggggttcagc ctgcacgaag agctggcgct gttt
//
LOCUS       DATLKL010000090         2254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_125073, whole genome shotgun sequence.
ACCESSION   DATLKL010000090 DATLKL010000000
VERSION     DATLKL010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125073"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VM713_01180"
     CDS             <1..524
                     /locus_tag="VM713_01180"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HVH82904.1"
                     /translation="DVPQRALYEVWRELAGLGLAMPNIGTLTDMIACPGLDFCSLANA
                     GTITVARQIQERFDNLDYLYDLGEIEIKMSGCMNACGHHHVGHIGILGVDKHGEEWYQ
                     ISLGGSANGFTALGEVIGPSVPKQEVAATILRIVEVYRARRVEGEPFIETLRRVGLEP
                     FRAGAYALHPQAA"
     gene            487..990
                     /locus_tag="VM713_01185"
     CDS             487..990
                     /locus_tag="VM713_01185"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HVH82905.1"
                     /translation="MPVPMRYILRRREIVADDWSHLGEELAADAALIVPLAELRADAA
                     RWGAWRGPLGVRLAPGDAPEDLAAQLPRLALVAIEFPNPGDGRGYSQARLLRTRLGFR
                     GELRAVGPGVRQDQVFLLARCGFDAIELAPGEDLEAARRALTRYTVAYQPGAAGAATG
                     RQRFSTP"
     gene            complement(997..1968)
                     /gene="cysB"
                     /locus_tag="VM713_01190"
     CDS             complement(997..1968)
                     /gene="cysB"
                     /locus_tag="VM713_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012313669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HVH82906.1"
                     /translation="MKLHQLRYLAAVAQSGLNITAAAHKLHTSQPGVSKQIKLLEDEL
                     GFQIFVREGRNLTRITAAGQQVIERALRILQEAQSIRDLSTELRDEGKGSLSIGTTHT
                     QARYVLPDVIREFRGRYPQVRLNLHQGTSEQIAEMVAGDRIDCAIATGSESLFSDLTL
                     LPCYRWHRTVIVPRGHALAGGGKLTLRALSAYPLITYTFSFTGPSSLHEAFARAALIP
                     NVAITARDADVIQTYVRLGLGVGIVAHMAIDPADPDLVAVDASQLFAAHTTWLGFRRG
                     TLLRKYMYDFAQLLAPHLDRRLVDRAHRATSAAEVAQLFSEIALPER"
BASE COUNT          331 a          772 c          778 g          373 t
ORIGIN      
        1 cggatgtgcc gcagcgcgcc ctttacgagg tgtggcgtga gctcgctggg ctcggcctcg
       61 caatgccaaa catcggcacc ctcaccgaca tgatcgcctg ccccggactc gacttctgct
      121 cgctcgccaa tgccggcacg atcacggtgg cgcggcagat ccaggagcgc ttcgataatc
      181 tcgactatct ctacgacctc ggtgagatcg agatcaagat gtccggttgc atgaatgcct
      241 gcggccacca tcacgtcggt cacatcggca tactcggcgt cgacaagcac ggcgaggagt
      301 ggtaccagat cagcctcggc ggctccgcca atggcttcac cgcgctcggc gaggtgatcg
      361 gcccgtcggt gccgaagcag gaggtcgcgg cgacgatcct gcggatcgtc gaggtgtatc
      421 gcgcccgacg cgtcgagggc gagcccttca tcgagacgct gcgccgcgtt ggactcgagc
      481 ccttccgtgc cggtgcctat gcgctacatc ctcaggcggc gtgagatcgt tgcggacgac
      541 tggagccatc tcggcgagga gctcgccgcg gatgccgcgc tcatcgttcc tctcgccgag
      601 ctgcgtgccg acgcggcgcg ctggggcgcc tggcgcgggc cgctcggcgt gcgcctcgcc
      661 cccggcgacg ctcccgagga tctcgccgcg cagctcccgc gccttgctct ggtcgcgatc
      721 gagttcccca accccggcga tggccgcggc tactcccagg cccggctgct gcgcacgcgc
      781 ctcggcttcc gcggcgagct gcgtgccgtg gggccggggg tgaggcagga tcaggtgttc
      841 ctgctcgcgc gctgcgggtt cgacgccatc gagcttgccc cgggcgagga cctcgaggcc
      901 gcgcgccgcg ctctgacgcg ttatacggtc gcctatcagc cgggtgccgc tggcgctgcg
      961 acggggcggc agcgcttttc gacgccctga ttccctctac cgctccggca gcgcgatctc
     1021 gctgaagagc tgcgcgacct ccgccgccga ggtggcgcga tgcgcccgat cgaccagccg
     1081 gcggtcgaga tgcggcgcga gcagctgcgc gaagtcatac atgtacttgc gcagcaacgt
     1141 cccgcgccgg aagccgagcc aggtcgtgtg ggcggcgaac agctgcgagg catcgaccgc
     1201 caccagatcg ggatccgcgg gatcgatcgc catgtgcgcc acgatgccga cgccgagccc
     1261 aagccgcacg taggtctgaa tcacgtcggc atcgcgtgcc gtgatggcga cgttcgggat
     1321 cagcgccgcc ctggcgaacg cctcgtgcag cgacgacggt cccgtgaaac tgaaggtgta
     1381 ggtgatgagc ggatacgctg acagggcgcg cagcgtgagc ttgccgccgc cggcgagcgc
     1441 gtggccgcgg ggaacgatca cggtgcgatg ccagcggtag cagggcagca gtgtgagatc
     1501 gctgaacagc gactccgaac cggtggcgat ggcgcagtcg atccgatcac ccgccaccat
     1561 ctccgcgatc tgctcggagg tcccctggtg gaggttgaga cgcacctgcg ggtagcgccc
     1621 gcggaactcg cggatgacgt ccggcagcac atagcgcgcc tgcgtatgtg tcgtgccgat
     1681 cgacaggctt cccttgcctt cgtcgcgcag ctccgtcgac aggtcgcgga tgctctgcgc
     1741 ctcctgcagg atgcgcagcg cccgctcgat cacctgctgg cctgccgcgg tgatgcgggt
     1801 gagattgcgg ccctcgcgga cgaaaatctg aaagccgagc tcgtcctcga gcagcttgat
     1861 ctgcttgctg acgcctggct gtgaggtgtg cagcttgtgg gccgccgccg tgatgttgag
     1921 accgctctgg gcgaccgccg ccaggtaacg cagttgatgc agcttcatct ggcgctcaag
     1981 gctaccgccc cgtggcaggc ctgtcaccgc cataaccgga gcgcagtacc taagaaggaa
     2041 tccttcgttc agcgctcggg gtcggcgcgg ctatagtcgg cctatcatgg gagaggtgat
     2101 cccgcccgcg tccatggact acctcccgct gttcctgcgg ctgcaaggcg ccccggccgt
     2161 cgtcgtcggt ggcggccacg tcgccgcgcg caaggccgag tggctcctca aggcgggcgc
     2221 acgggtgacc gtggtcgccc cgcagctcgg cacg
//
LOCUS       DATLKL010000091         2268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_136315, whole genome shotgun sequence.
ACCESSION   DATLKL010000091 DATLKL010000000
VERSION     DATLKL010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136315"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2249)
                     /locus_tag="VM713_01195"
     CDS             complement(<1..2249)
                     /locus_tag="VM713_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH82907.1"
                     /translation="MRSSSTFGLSLITVLFRDGYEDYWVRQRLLERINQVTLPAGAAA
                     SLDPVAGPGGEIYRYTLESDTRNLQELSEIQRWTVIPALKSVPGVVDDTNFGGFTTEY
                     QLDLDPKQLEHYGLGITDVINAINSNNANAAGGRVSRGDQSYVIRGVGLLRSLTDIGA
                     TVVSQVNGAPVRVRDLGRTTLSHQEREGILGKDNNPDTIEGIVLMLKYQNPSETLKGI
                     HARVAELNRQLAAQDVRIVPYIDRDDLVRATVHKVGRTILEGISLVFLILILFLGSPR
                     SALVAGVSIPLALVAVFSLLNLTHVSANLLSLGAIDFGILVDGAIVVTEAILRRREAM
                     PTEELSESEVKAAAVQVTRPIFFASLIIITAYLPLFSFERAEAKLFTPMAYTMAYALF
                     GALLTTLALVPGLAYYAFRKPGPVYHNRWLEWLTLRYERILAACIDQPRIVYGIAIFA
                     LLAVLGLGATVGRDFLPDLDEGALWLQVQMPTGLSLNKASDMASELRREIRKFPEVSY
                     VVTQTGRNDEGTDPWTMSHIEAPVGLTPYDTWPHAESKRQFLDKLAATLARDLPGYSV
                     GISQPIIDGVNDMIGGAHSPLVIKIYGDDLAMLRRVGQEMVDVLYTIRGTSSASIFQE
                     PPIPQINISVDREKTARYGINIADVQNVIQTGVGQAPVSTVYVGERVYNLTVRFVATS
                     RDSPEALGSLPVHTASGAQIPLSQAADIRVQSGESTITRENGQRNLTIRIDNRDRDLT
                     SYLEEAQAK"
BASE COUNT          375 a          697 c          801 g          395 t
ORIGIN      
        1 atcttcgcct gcgcctcctc gaggtagctg gtcaggtcgc ggtcgcggtt atcgatgcgg
       61 atggtgagat tgcgctgccc gttctcgcgc gtgatggtgc tctcccccga ctgcacgcgg
      121 atgtcggccg cctgtgacag gggtatctgc gcaccgctcg cggtgtgcac cgggaggctc
      181 ccgagtgcct cgggcgaatc gcggctcgtg gcgacgaagc gtaccgtgag gttgtagaca
      241 cgctcgccga catacaccgt cgacaccggc gcctgcccga cgcccgtctg gatgacgttc
      301 tgcacgtcgg cgatgttgat cccgtagcgc gccgttttct cgcggtccac ggagatattg
      361 atctgcggaa tcggcggctc ctggaagatc gaggcgctgg aggtgccgcg gatggtatag
      421 aggacgtcta ccatctcctg gcccaccctg cgcagcatcg cgaggtcgtc gccgtagatc
      481 ttgatgacca gcgggctgtg cgcaccgccg atcatgtcgt tgacgccgtc gatgatcggc
      541 tggctgatgc cgaccgaata gcccggcagg tcgcgcgcca gagtggcggc gagcttgtcg
      601 agaaactgcc gcttgctctc cgcgtgcggc caggtgtcgt aaggggtgag ccccaccggc
      661 gcctcgatgt gcgacatggt ccaggggtcg gtgccctcgt cattgcggcc ggtttgggtg
      721 acgacgtacg agacctcggg gaacttacgg atctcgcgcc gcagctcgct cgccatatcg
      781 ctggccttgt tgagagacag ccccgtcggc atctgaacct gcagccacag agctccctcg
      841 tcgaggtcgg gaagaaaatc ccgccccacc gtcgccccga gcccgagcac agcgagcagc
      901 gcgaagatcg cgatgccata gacgatgcgc ggctgatcga tgcaggcagc cagaatgcgc
      961 tcgtagcgca gggtcagcca ctcgagccag cggttgtgat agacggggcc cggcttgcgg
     1021 aaggcgtaat aggcaagccc gggcaccagt gccagtgtcg tgagcagcgc cccaaacagc
     1081 gcgtacgcca tggtgtaggc catgggggtg aacagcttcg cctcggcgcg ctcgaaggag
     1141 aagagcggca ggtaggcggt aatgatgatg aggctcgcga agaagatcgg gcgggtcacc
     1201 tgcaccgctg cggccttgac ctcgctctct gagagctcct cggtcggcat agcctcgcgg
     1261 cgccgcaaga tcgcctcggt gacgacgatg gcaccatcta cgaggatgcc gaagtcgatc
     1321 gcgccgagcg acagcaggtt cgccgagacg tgcgtgaggt tcaggagcga gaacaccgcc
     1381 accagcgcga gcggaatcga cacgccggcg accagcgcgc tgcgcgggct gccgagaaac
     1441 aggatgagga tgaggaatac gaggctgatc ccctcgagga tcgtgcggcc gaccttgtgc
     1501 acggtggcac gcaccaggtc gtcgcggtcg atgtagggca cgatgcgcac gtcctgggcg
     1561 gcgagctgac ggttgagctc cgcgaccctg gcgtggatgc ccttcagggt ctccgacggg
     1621 ttctgatact tcagcatcag cacgatgcct tcgatggtgt cggggttgtt gtccttgccg
     1681 aggatgcctt cgcgttcctg gtggctcaga gtcgtacggc cgagatcgcg gacccgcacg
     1741 ggcgcgccgt tcacctggct tacgaccgtc gcgccgatgt cggtgaggct gcgcaggagg
     1801 ccgacgccgc ggatgacgta gctctggtcg ccgcgcgata cgcggccgcc ggcggcgttg
     1861 gcgttgttgc tgttgatggc gttgatgacg tcggtgatgc cgagcccgta gtgctcgagc
     1921 tgctttgggt cgaggtcgag ctggtactcg gtggtgaagc caccgaagtt ggtgtcgtcg
     1981 accacgcccg gcacggactt gagcgccgga atgaccgtcc agcgctggat ctcggagagc
     2041 tcctggagat tgcgggtgtc ggactcgagg gtgtagcggt agatctcgcc gcccggtccg
     2101 gccaccgggt cgagcgaggc tgcggcaccc gccggcagcg tcacctggtt gatgcgctcg
     2161 agcaggcgct gccggaccca gtagtcctca tagccgtcac ggaacaacac cgtgatcagc
     2221 gacagcccga aggtgctgga agagcgcatc agcagcagcc ccggggtg
//
LOCUS       DATLKL010000092         2240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_158817, whole genome shotgun sequence.
ACCESSION   DATLKL010000092 DATLKL010000000
VERSION     DATLKL010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158817"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..330)
                     /locus_tag="VM713_01200"
     CDS             complement(208..330)
                     /locus_tag="VM713_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82908.1"
                     /translation="MLLAPLLLLPLIATALRSVPLLGTRGGLSAGLRGFISAWL"
     gene            complement(389..1048)
                     /locus_tag="VM713_01205"
     CDS             complement(389..1048)
                     /locus_tag="VM713_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82909.1"
                     /translation="MSREAVEVETGANPTAAVIWLHGLGADGHDFEPIVPELVSPGER
                     ALRFVFPHAPIRPVTLNGGFAMRAWYDIVSLERRGPEDEAGIRASQAAVETLIRRENA
                     RGIATSRIVLAGFSQGGSVALFAGTRYGEKLAGIMGLSCYLLLAGRLAAERGSANQAT
                     PIFLAHGLEDPIVAPLLGEQARAALEGAGYPVEWHTYPMPHSVSPQEVGDIAQWLRRV
                     L"
     gene            1256..1981
                     /locus_tag="VM713_01210"
     CDS             1256..1981
                     /locus_tag="VM713_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82910.1"
                     /translation="MQLTIVLAAATVLALAVNGLFQLLRKPTELFFPVSGALYKTPAE
                     TWRDYAPLFRRYSTAAISPELLAALAQVEGSGNPLVRTYWRWSWTRQPFEVYRPASSA
                     VGMYQITDGTFEVARHYCIHHHVLTREGPWNDLDSCWFNRLYVRVLPGDAVELTAAYL
                     DLELANVLGPHRARTAPAELRHVAAVVHLCGAGAAESNVRRGLRFGEGERCGDHDQRA
                     YLERVDGTYGVFARLALGDAAPQ"
     gene            complement(1975..>2240)
                     /locus_tag="VM713_01215"
     CDS             complement(1975..>2240)
                     /locus_tag="VM713_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVH82911.1"
                     /translation="EYDRALVQKVPRRTLKGIANLKVGMRLQAGHQPVTVTHIAGDMV
                     TLDGNHPLAGQNLHFDVEVTAVRAATEEELAHGHVHGNGGHHH"
BASE COUNT          332 a          789 c          785 g          334 t
ORIGIN      
        1 ggggattatt cacagaaccg cggtaacagc ggctacccgc agggcgcgac ctcacagccg
       61 cgtggctact ccgagccccg tcgcgaggcc aatgcgcagc cgcggggcta ttcggaaccg
      121 cgtggcggag gcaatgcgca gcctcatccc ggcggatatt cacagccacg tgcgaatcaa
      181 cccccgcagt cgcgtcccgg cggttattca cagccacgcg gaaatgaagc cccgcagtcc
      241 cgcggacagc ccgccgcgcg tcccgagcag gggcacgctc ctcaaggccg tggcaatgag
      301 cggcaacagg agcaggggcg cgaggagcat ccgcactgat atcaaaatcg gggcgcgaca
      361 gggacgtcgc gttcccgacc gttcgaaact acagcacgcg gcgcagccac tgcgcgatgt
      421 cgccgacctc ctggggactg acggagtggg gcatcgggta ggtatgccac tcgacgggat
      481 agcctgcccc ctcgagcgcc gcgcgcgcct gctcaccgag gagcggcgct acgatcggat
      541 cctcgaggcc gtgcgcgagg aagatcggcg tagcctgatt cgcgctgccg cgctcggccg
      601 cgaggcgccc ggcgagcagc aggtagcagg agagccccat gatcccggcg agtttctcgc
      661 cgtagcgggt gccggcgaac agcgcgaccg acccgccctg ggagaatccg gcgagcacga
      721 tgcgcgaggt ggcgatgccg cgcgcgttct cgcggcggat cagcgtctcg accgccgcct
      781 gtgaggcgcg gatgccggcc tcgtcctcgg ggcctcggcg ctcgaggctc acgatgtcgt
      841 accaggcccg catggcgaag ccgccgttaa gcgtgaccgg ccggatcggc gcgtgcggaa
      901 acacgaagcg cagcgcgcgc tcgcccgggc tgaccagctc gggcacgatg ggctcgaaat
      961 catggccatc cgccccgagt ccatgcagcc agatcaccgc cgccgtcgga ttggcacccg
     1021 tttcgacctc gaccgcctcg cgtgacaccg acgactcctg actccgctgc caaggcacat
     1081 ggatgtgccc gccgccgcag gcggcgccat actagagcac ggcggtcgcg cgcggtaccg
     1141 aacgcagaga gccgcccatg agtacgcgcc gcgcgcgccg ccgtcgctgc ggccgtggcc
     1201 tcgagcacgc acctggcggc ggtggctgcg ggcgctgcgt cgcgcgcccc ggccgttgca
     1261 gctcacgatc gtgctcgccg cggcgactgt cctggcgctc gccgtcaacg gcctgttcca
     1321 gctcctgcgc aagcccaccg agctcttctt tcccgtgagt ggcgcgctct acaagacacc
     1381 cgcggagacc tggcgcgatt acgcaccgct ctttcggcgc tactcgaccg cggccatttc
     1441 gccggagctg ctcgccgcgc tagcccaggt ggagggatcg ggcaatccgc tggtgcgcac
     1501 gtactggcgc tggtcgtgga cacggcagcc cttcgaggtg taccggccgg cctcgagcgc
     1561 ggtcggcatg taccagataa ccgatggcac cttcgaggtg gcgcggcact attgcatcca
     1621 ccaccacgtc ctgactcgcg agggtccgtg gaacgactta gactcgtgct ggttcaaccg
     1681 gctgtacgtg cgtgtgctgc caggggatgc cgtggagctc acggcggcgt acctcgatct
     1741 cgagctggcg aatgtgctgg ggccgcaccg cgcccgcacc gcgcctgccg agctccgtca
     1801 cgtggcggcc gtggtgcacc tgtgcggggc cggcgccgca gaatccaacg tgcggcgggg
     1861 tctgcggttc ggcgagggcg agcgctgcgg cgatcacgac cagcgcgcct acctcgagcg
     1921 ggtcgatggg acgtacgggg tgtttgcgcg cctggcgctc ggggatgccg cccctcagtg
     1981 atggtggccg ccgttcccgt gcacgtggcc gtgggcgagc tcttcctcgg tcgcggcgcg
     2041 cacggcggtg acctcgacgt cgaagtgcag gttctgcccg gcgagcgggt ggttgccgtc
     2101 gagcgtcacc atgtcgccgg cgatgtgcgt caccgtcacc ggctggtggc ccgcctgcag
     2161 acgcatgccg accttcaggt tggcgatgcc cttcagggtt cgccgcggta ccttctgcac
     2221 cagggcacgg tcgtactcgc
//
LOCUS       DATLKL010000093         3201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_12213, whole genome shotgun sequence.
ACCESSION   DATLKL010000093 DATLKL010000000
VERSION     DATLKL010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12213"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..869)
                     /gene="lipB"
                     /locus_tag="VM713_01220"
     CDS             complement(180..869)
                     /gene="lipB"
                     /locus_tag="VM713_01220"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HVH82912.1"
                     /translation="MSAAGALAAAVVSAPVVAALEVPPALIRRLGRVEYAPTWRAMQR
                     FTEERGAATPDEIWLLEHPPVFTLGANASRTHLIAPGDIPVVQIDRGGQVTYHGPGQL
                     VVYPLIDLKRLRLGIRDFVTALERAVIDLAASYGIAAEARRNAPGVYVAGEKLASVGV
                     RVRRQGSYHGLALNVALDLEPFSRINPCGYQGLEMTQLASLGGPRTVWECADALEPHL
                     TRALGLSAVRD"
     gene            complement(866..1903)
                     /gene="lipA"
                     /locus_tag="VM713_01225"
     CDS             complement(866..1903)
                     /gene="lipA"
                     /locus_tag="VM713_01225"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HVH82913.1"
                     /translation="MERVPELKGIPVVTAGAAAPRSGEKYVTPQGFIAIRDGIKPARS
                     APAALPARKPPWLKARAPAGQGFQGVRAIVREHRLATVCEEAKCPNIGECWNAGTATL
                     MLMGAVCTRACRFCSVDTGNPHGWLDADEPENAARTVELMKLKYVVLTSVDRDDLADG
                     GAAHYAACVRAIRARNPETAVEALTPDFQGVLADVATVVDSGIAVFAQNIETVRRLTH
                     PVRDPRAGYEQTLSVLAHAKRHRPSILTKSSLMLGLGETDAEIAATLADLRAAQVDLV
                     TLGQYLRPTVHHLPVERFVTPAEFDRYREQALALGFLECVAGPLVRSSYRAEQALNRN
                     NAGLDNRSQQP"
     gene            complement(1908..2567)
                     /locus_tag="VM713_01230"
     CDS             complement(1908..2567)
                     /locus_tag="VM713_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HVH82914.1"
                     /translation="MLLAAGRGERMRPLTDSLPKPLVPVAGKPLIAWHLAALARAGFR
                     EVVVNTSWLAEQLHVALGDGSAWGVSITWSDEGPVPLETGGGIFRAVPLLGPGPFLVV
                     NADIWTDIDFGALVLEQGAHAHLVLVPNPPHNVRGDFGLDGDLVVSRDTDRFTYSGVG
                     VFRREFFDGASGERFPLLPLLNRAIAARRVRGELHRGAWSDVGTAERLAALDARQRAL
                     Q"
     gene            complement(2590..>3201)
                     /locus_tag="VM713_01235"
     CDS             complement(2590..>3201)
                     /locus_tag="VM713_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HVH82915.1"
                     /translation="ALMPEWLLRRHLALEPSPGEAELIADSFAFLIREGLAQPEVFVH
                     RDYHSRNLMLMSARNPGVIDFQDALRGPVGYDLVSLLKDCYIAWPRARVAAWVSGYRA
                     QLGRAGAAAGASESEFLRWFDLIGVQRHIKVLGIFCRLWYRDGKAGYLADLPRVLAYV
                     RETCGEYAELASLGRFLEERVAGALPRANARVLGARPGAAVRA"
BASE COUNT          490 a         1132 c         1146 g          433 t
ORIGIN      
        1 tattccgcgt acagctcgcg cgtcggatcg taggagacgt tgaggagcgt cacgcgcgtg
       61 gtggagctgt gcggcccgcg gtcgcagccg ccgagcccgg cgagcagcat cagcccggcg
      121 agcaggagtc ggcgaacgca cggcatcatc gcagcgtcgg cgcggtacgc agcgcgccgt
      181 cagtcgcgga cagctgacag cccgagcgcg cgcgtgagat gcggctcgag cgcgtcggcg
      241 cactcccaaa ccgtgcgcgg accgccgagc gatgcgagct gcgtcatctc gaggccctgg
      301 tagccgcagg ggttgatgcg cgagaacggc tcgagatcca gcgccacgtt gagcgcgagg
      361 ccgtgatagc tcccctggcg ccgcacgcgc acgccgacgc tcgcgagctt ctcgccggcg
      421 acatataccc cgggcgcgtt gcggcgggct tccgcggcga tgccgtaact ggctgcgagg
      481 tcgatcactg cgcgctcgag cgcggtgacg aagtcgcgga tacccaacct caagcgcttg
      541 aggtcaatga gcgggtacac caccagctgc cccgggccgt ggtaggtcac ctgtccgccg
      601 cgatcgatct gcaccaccgg aatgtcgccg ggcgcgatga ggtgcgtgcg gctcgcgttg
      661 gcgccgagcg tgaacaccgg cgggtgctcg aggagccaga tctcatctgg cgtcgccgca
      721 ccgcgctcct cggtgaagcg ctgcatcgcg cgccaggtgg gcgcgtactc gacgcgcccg
      781 agccgacgga tgagcgcggg cgggacctcg agggccgcca ccaccggtgc ggacaccacc
      841 gccgccgcga gcgcgccggc ggcgctcacg gctgctggct ccggttgtcg agcccggcgt
      901 tattgcggtt gagcgcctgc tccgcccgat agctcgagcg caccagcggc cccgccacgc
      961 actcgagaaa gccgagcgcg agcgcctgct cgcggtagcg atcgaattcg gcgggcgtca
     1021 cgaagcgctc gacgggaaga tggtgcaccg tcgggcgcag atactgcccg agcgtcacga
     1081 ggtcgacctg cgcggcgcgc aggtcggcga gcgtcgccgc gatctccgcg tcggtctcgc
     1141 cgaggcccag catcaggctg ctcttggtga ggatcgacgg ccgatggcgc ttggcgtgcg
     1201 cgagcacgct gagcgtctgc tcgtagccgg cgcgcggatc gcgcactgga tgcgtcagcc
     1261 gccgcacggt ctcgatgttc tgcgcgaaca cggcgatgcc ggagtcgacc accgtggcga
     1321 cgtcggccag cactccctgg aaatcgggcg tcagcgcttc gacggcggtc tcggggttgc
     1381 gcgcgcggat ggcgcgcacg caggcggcgt agtgcgcggc gccgccgtcg gcaagatcgt
     1441 cgcggtcgac cgaggtgagc accacgtatt tgagcttcat cagctccacg gtccgcgcgg
     1501 cgttctcggg ctcatcggcg tccagccacc cgtgcgggtt gccggtgtcc accgagcaga
     1561 agcggcaggc gcgggtacac accgcgccca tcagcatcag cgtcgcggtg ccggcgttcc
     1621 agcactcgcc gatgttgggg cacttggcct cctcgcacac cgtggcgagc cggtgctcgc
     1681 gcacgatggc gcgcacaccc tgaaaacctt ggcccgcggg cgcgcgggcc ttgagccagg
     1741 gcggctttct cgccggcagg gcggcgggcg cggatctggc cggcttgatg ccatcccgga
     1801 tagctataaa cccttggggt gtaacgtatt tttcgccgct gcgcggggcc gcggcgccgg
     1861 ccgttacgac cggaattcct ttgagctcgg gcacgcgttc cacgcgttca ttgtagcgca
     1921 cgctgccgcg catccagcgc cgcgaggcgc tcggcggtcc ccacgtcgct ccaggcaccg
     1981 cggtgcagct cgccgcgcac ccggcgggcg gcgatcgcgc ggttcaggag cggcagcagc
     2041 gggaagcgct cgccgctcgc gccgtcgaag aactcgcgcc ggaacacgcc aacgcccgaa
     2101 taggtgaagc ggtccgtgtc gcgactgacc acgaggtcac cgtcgagccc gaaatccccg
     2161 cgcacgttgt gcggcggatt gggtaccagc acgagatgcg cgtgcgcgcc ctgctcgagc
     2221 acgagcgcgc cgaaatcgat gtcggtccag atgtcggcgt tcaccaccag aaatgggccg
     2281 ggcccgagga gcggtaccgc gcggaagatg ccgccgccgg tctcgagcgg caccgggccc
     2341 tcgtcgctcc aggtgatcga gacgccccag gcgctgccgt cgccgagcgc cacgtgcagc
     2401 tgctcggcga gccacgaggt attgacgacg acctcacgga aacccgcgcg ggcgagcgcg
     2461 gcgaggtgcc aggcgatgag gggcttgccg gcgactggca ccagcggctt gggcagcgaa
     2521 tcggtgagcg gccgcatccg ctcgccccgg cccgccgcga gcagcatggc cttcacgcac
     2581 gcaccgcctt cacgcgcgca ccgcggcccc gggccgagcg cccaggaccc gcgcgttggc
     2641 acgtggcagc gcaccggcaa cccgctcctc gagaaagcgc ccgagggacg cgagctccgc
     2701 gtactcaccg caggtctcgc gcacgtaggc aagcacgcgt ggcaggtcgg cgaggtagcc
     2761 ggccttgccg tcgcggtacc acagccggca gaagatgccg agcaccttga tgtgccgctg
     2821 tacgccgatg aggtcgaacc agcgcaggaa ctcactctcg ctcgccccgg ccgcggcacc
     2881 cgcgcgcccg agctgcgcgc ggtagccact cacccaggcc gccacccgcg cgcgcggcca
     2941 cgcgatataa cagtccttga ggagcgacac cagatcgtag cctacgggtc cgcgcaacgc
     3001 gtcctggaag tcgatcacgc cgggattgcg ggcgctcatc agcatgagat tgcgcgagtg
     3061 atagtcgcga tgcacgaaca cctcgggctg cgccaggccc tcgcgaatga ggaacgcgaa
     3121 ggagtcggcg atcagctccg cttcgcccgg cgacggctcg agcgcgaggt gccgcctgag
     3181 cagccactcg ggcatcagcg c
//
LOCUS       DATLKL010000094         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_125164, whole genome shotgun sequence.
ACCESSION   DATLKL010000094 DATLKL010000000
VERSION     DATLKL010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125164"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VM713_01240"
     CDS             <1..273
                     /locus_tag="VM713_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVH82916.1"
                     /translation="MRYRTSRMRPAFLTPNQVYEVTIDLWATSHVFLPGHRVRLEISS
                     SNFPRFDRNLNTGEDQATGSRRQTAAQTILHDQRCPSHILLPVIPR"
     gene            complement(243..1718)
                     /locus_tag="VM713_01245"
     CDS             complement(243..1718)
                     /locus_tag="VM713_01245"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HVH82917.1"
                     /translation="MASLIVWMNGERVGEWGTLRGGRTPFFRYAESWVRSPHGRAVSL
                     SLPITADLEIRGSAVEYYFDNLLPDSSAIRGRIRSRFRARSADAFDLLSAIGRDCIGA
                     VQLLPPTEEPVGWDRIHAQALTDEEVAQALREVTLTTPFGHSEQMGFPISLSGDQEKT
                     ALLHMAGLWYRPRGATPTTHILKLPLGGVGGFHGDFSDSVENEWLCAQLLRELGLPVA
                     EVAIGRFAEQRALIVSRFDRRWIGVDAAEIHTPRFKPRRGMWIARLPQDDFCQALGLP
                     NTLRYAADDGPSALVGLQLLATSEAPHVDQTHFALAQLAFWLLAATDGHAKNFSLYYG
                     PGARYRMTPLYNVISAWPIIDHGKNQLALEKAELAMAVHGRRARYRMSEITSHDWQAF
                     AEDTGTPELWARMQSSTGRAPQARREISRNSRCGSPRPWSPRTNASLGELAAEGKGLA
                     PRYSRHLARQMSCCTDPGRLLRRVSPGSEEIGYLGITGSNM"
BASE COUNT          372 a          678 c          677 g          357 t
ORIGIN      
        1 atgcgttacc gtacctcgcg tatgcgcccg gcatttctca caccgaacca ggtctacgag
       61 gtgacaatcg acctatgggc tacaagccac gtgtttttac cggggcatcg cgtgcgcctg
      121 gagatttctt ccagcaattt cccacgcttc gaccgcaacc tgaacactgg tgaggaccag
      181 gccacgggca gccgccggca gacagcggct cagaccattc tgcatgacca acgttgcccg
      241 tctcacatat tgctgccggt gataccgagg tagccgatct cctcggagcc cggagagacc
      301 cgcctcaaca accgtccagg atcggtgcag cagctcattt gcctcgcaag atggcgagag
      361 taccgaggtg ctaatccctt tccctccgct gcgagctccc cgagcgacgc attcgtccgc
      421 ggcgaccacg gccgcggaga accgcagcgg ctattccgac tgatctctcg tcgggcctgc
      481 ggagcccgac cagtcgagga ctgcatacgc gcccagagct cgggcgttcc cgtgtcctcg
      541 gcgaaggcct gccagtcgtg cgaggtgatc tcgctcatcc ggtaacgcgc tctacgcccg
      601 tgtaccgcca tggcgagctc cgccttctcg agcgccagct gattcttgcc gtggtcgatg
      661 atcggccagg ctgaaatgac gttgtaaagc ggcgtcatcc gatacctggc gcccgggccg
      721 tagtaaaggg aaaagttctt ggcgtgtccg tcggtggcgg cgagcagcca gaaggcgagc
      781 tgcgcaagcg cgaaatgagt ctgatccacg tgaggcgcct cgctggttgc aagaagctgc
      841 agccccacca gcgccgatgg tccgtcgtca gccgcatagc gcagcgtatt gggtagacct
      901 aaggcctgac agaaatcgtc ctgcggcagc cttgcaatcc acatgcctcg gcgcggtttg
      961 aaacgaggtg tgtggatctc ggcggcgtcc acaccgatcc agcggcggtc gaagcgcgac
     1021 acgatgagcg cgcgctgctc cgcgaagcgc ccgatcgcca cctccgcaac gggcaggccg
     1081 agctctcgca ggagctgtgc gcagagccac tcgttctcga ccgagtccga gaaatccccg
     1141 tggaagccgc cgacacctcc gagggggagc ttcaggatgt gggtcgtcgg ggtcgcgcca
     1201 cgcggccggt accagaggcc tgccatgtga aggagcgccg tcttctcctg gtcgccagag
     1261 agcgaaatgg ggaagcccat ctgttcggaa tggccgaacg gggtggtgag ggtcacctct
     1321 ctcagggctt gcgccacctc ctcgtcggtc agggcctgag cgtggatccg atcccagccc
     1381 acgggctctt cggtcggagg caagagctgt acggccccga tgcaatcgcg gccgatggcg
     1441 ctcagcaggt caaaggcatc ggcggaccgc gcgcggaagc gactgcggat gcggccgcgg
     1501 atcgcagagc tgtcgggcag gaggttgtca aagtaatact ccaccgccga tcctcggatc
     1561 tccaggtcgg cggtgatcgg cagcgacagc gacacggccc gcccgtgggg tgagcgaacc
     1621 cacgactcgg cgtagcgaaa gaaaggcgtg cggccgcccc ggagggtgcc ccactctccc
     1681 acccgctcgc cattcatcca tacgatgagc gaggccacgc gcctctacca gtcgatcggt
     1741 gcgccgcggc gttgctcctc gcctcggtcg tccgctccct cgagcagtac gcgtacccgc
     1801 agcaccgcga gcagcccgag cagctgcccg acgctgacgc gactcgggtt gcgctcgatc
     1861 ttcgcgatgc gggtctggtc gaggcccgcg agcttaccga gcgcggcctg ggtgagtccc
     1921 cgcgtcttgc ggaacgagcg cagatggctc gccagttgcg ccggggtctg caggatcagg
     1981 ggcttctagg tgtgagcaga atatgccttt agatgcatgt atgcaataca tgacttatgc
     2041 ggcatatagg gtgatcggac tgggcgacgg cgcggcactg gtgc
//
LOCUS       DATLKL010000095         2584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_46094, whole genome shotgun sequence.
ACCESSION   DATLKL010000095 DATLKL010000000
VERSION     DATLKL010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46094"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1797
                     /locus_tag="VM713_01250"
     CDS             130..1797
                     /locus_tag="VM713_01250"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: protein motif:HMM:NF008594.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HVH82918.1"
                     /translation="MSDRPASRPLATHEVLNQPPPFEDVNFYLTDRALRDAVKREGGA
                     IAEDRHKALGAAVGSAEVMKAAEQANRWPPELRTHDRFGHRIDEVEFHPAWHALMELA
                     VAHRVPSFAWVEKRQGAHVARAALAFLWGQAEAGVSCPMAMTFAAMPALRRQPEIAAE
                     WEPRLLSDKYDSRFMPAKGKTGALMGMAMTEKQGGSDVRANTTRARALGKRGPGGEYL
                     LTGHKWFCSAPMSDAFLTLAHAEGGLSCFFAPRFLPDGTKNRILLQRLKNKLGNRSNA
                     SAEIEYDDTWARLIGEEGRGVQAIIEMVQGTRLECAIGSAALMRQALAQAIHHASHRS
                     AFHKRLVEHSLMQNVLADMALESEAATQLAFRVARAFDEGANDERARRFARIATPVAK
                     YWICKRAPVLVGEALECLGGNGYIEESMMPRLYREAPLNSIWEGSGNVICLDVARAIE
                     QEPEAVSALFAELDDARGADQRYDWWIDRLKEEFAERTAFEARARRFVEAAALALQAS
                     LLIRRGPAAMADAFCISRLAGGHGSTFGTLPAGVDLAAMIERARPVE"
     gene            1909..>2584
                     /locus_tag="VM713_01255"
     CDS             1909..>2584
                     /locus_tag="VM713_01255"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HVH82919.1"
                     /translation="MTGAPRASYRSDGQEAGEEAIEEAMRKNLKIDGERLWQSLMEMA
                     KIGATPKGGVCRIALTDLDRQGRDLFVRWCEEAGCRVTVDAIGNIFARRPGSDDSLPP
                     IVTGSHLDSQPTGGKFDGAYGVLAGLEVLRSLNDLDIKTKAPIEAVVWTNEEGARFAP
                     AMTASGVFAGKFALDYALALKDKDGTSFGEALAKIGYMGKAPVGGRPVGAFFEAHIEQ
                     GPILEAE"
BASE COUNT          436 a          870 c          875 g          403 t
ORIGIN      
        1 ctgaggggtt tacgccgcgt taatcagctg cctgcataga tggtggagct tggaacgttc
       61 ccgttacatt cggcgcgaca aactcggtgt gctatgaagg aagccacgac ggctgcccgc
      121 gaggatgaca tgagcgaccg gcccgcgtcg cgccccctgg ccacgcacga ggtgctcaac
      181 cagccgccgc cctttgagga cgtcaatttc tacctcaccg accgggcgct acgggacgcg
      241 gtcaagcgtg aggggggcgc catcgccgag gatcggcaca aggccctcgg cgccgcggta
      301 ggcagtgccg aggtgatgaa ggccgcggag caggccaatc gatggccgcc cgaacttagg
      361 acccacgacc gttttggcca ccgcatcgac gaggtcgagt tccacccggc ctggcatgcc
      421 ctcatggaac tcgccgttgc ccatcgggtg ccaagcttcg cctgggtcga gaagcgacag
      481 ggtgcccacg tcgcgcgcgc cgcgcttgct tttctgtggg gccaagccga agccggggtg
      541 agctgcccga tggcgatgac gtttgccgcc atgccggccc tgcggcgcca gccggagatc
      601 gcggcggaat gggagccacg gctgctctcc gacaaatacg actcccgctt catgcccgcc
      661 aagggcaaga ccggcgccct catgggcatg gcgatgaccg agaagcaggg cggctcggac
      721 gtgcgcgcca atacgacccg cgcccgcgcc ttgggcaagc ggggtccagg cggtgagtat
      781 ctcctgactg gtcacaaatg gttctgctcg gcgcccatgt cggatgcctt cctaaccctc
      841 gctcacgccg agggcgggct ttcctgcttc ttcgcgccgc gcttccttcc cgacggcacg
      901 aagaaccgca tcctcctcca gcggctcaaa aacaagctcg gcaatcgctc caacgcctcc
      961 gccgagatcg agtacgacga cacctgggcg aggttgatcg gcgaagaggg gcgtggggtg
     1021 caggccatca ttgagatggt gcagggcacg cgcctcgaat gcgcgatcgg ctcagcagcc
     1081 ctgatgcgcc aggcgctcgc ccaggcgatc caccatgcct cgcaccgcag cgccttccac
     1141 aagcgcctcg tggaacattc cctcatgcag aacgtgctcg ccgatatggc gctcgagtcc
     1201 gaagctgcga cgcagctcgc tttccgggtc gcgcgcgcat ttgacgaagg cgctaacgat
     1261 gagcgcgcgc ggcgcttcgc tcggatcgcc accccggtcg cgaagtactg gatctgcaag
     1321 cgcgcgccgg ttctcgtggg tgaggcgctc gagtgcctcg gcggcaacgg ttatatcgag
     1381 gagagcatga tgccgcggct ctaccgcgag gcgccgctca acagcatctg ggagggctca
     1441 ggcaacgtga tctgcctcga tgtggcgcgg gcgatcgagc aggagcccga ggcggtgagc
     1501 gcgctcttcg ccgagctaga cgacgcccgc ggcgccgatc agcgctacga ctggtggatc
     1561 gaccggctca aggaggagtt cgccgagcga accgccttcg aggcacgcgc gcgacgcttc
     1621 gtcgaggcgg cggcgctcgc gctgcaggcc tcgctcctga tccgccgcgg ccccgccgcg
     1681 atggcggacg cattctgcat ctcgcggctt gcgggcggcc acggcagcac cttcggcacg
     1741 ctgcccgctg gcgtcgatct cgccgcgatg atcgagcggg cgcggcccgt agagtaagct
     1801 cgccaccacg acagcacata gcgacggagt cgagacaggc tggatggcgc cggctttatc
     1861 gtgccgtcgt tgcggttcgc ctccgctact tttctcgctt accggccctt gaccggcgct
     1921 cctcgcgcct cctaccgttc ggacggccag gaggccggag aggaagccat agaggaggcc
     1981 atgcgcaaga acctcaagat cgacggcgag cggctgtggc agagcctgat ggagatggcg
     2041 aagatcggcg ccacgcccaa gggcggggta tgccgcatcg ccctcacgga cctcgaccgc
     2101 cagggccgcg acctgttcgt gcgctggtgc gaggaggccg gctgcagggt cacggtcgac
     2161 gcgatcggca acatattcgc gcgccggccc ggcagcgacg actcgcttcc gccgatcgta
     2221 accggcagcc acctcgacag ccagcccact ggtggcaagt tcgacggcgc atacggggtt
     2281 ctcgccgggc tcgaggtgct gcgcagcctg aacgatctcg acatcaagac caaggcgccg
     2341 atcgaggcgg tggtgtggac caacgaggag ggcgcgcggt tcgctcctgc catgaccgcc
     2401 tccggcgtct tcgctggcaa gtttgcgctc gactacgcgc tcgccctcaa ggacaaagac
     2461 ggaacgagct tcggcgaggc cctcgcgaag atcggctata tgggcaaggc gccggtcggc
     2521 ggccggcccg ttggcgcctt cttcgaggcc catattgagc aggggccgat cctcgaagcg
     2581 gaga
//
LOCUS       DATLKL010000096         2366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_12290, whole genome shotgun sequence.
ACCESSION   DATLKL010000096 DATLKL010000000
VERSION     DATLKL010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12290"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2006)
                     /locus_tag="VM713_01260"
     CDS             complement(<1..2006)
                     /locus_tag="VM713_01260"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HVH82920.1"
                     /translation="MLNRNDIHFPPKHEPLRDDVHALGALLGEILREQGGEPLFELVE
                     LDRVAAIRSRAGESDARAELAAHVRDRPPALARDLVRAFATWFQAVNLAERVHRIRRR
                     RDYLVKDSQRPQPGGVEDAIAGLRQEGWALGEVLELIQSLSSEAVFAAHSTDSNRRTL
                     LRKEQRIAERLLERLDPALTPGEQRDLWSSIRTEMTTAWQTEELPRERLTVADEREQI
                     LFYLLEILYQVVPAFYEEIAQALAKVYGPEAAVVRVPAILHFGSWAGGDTDGNPDVHA
                     KTIRESLARQQQVLVNSYFAECQSLAQRLSQSASRVAISAELAQRIERYTALLPAARA
                     TTRMHHERMPYRLFLAQVGERLRHAYDGRAYGYEGPQQFRADIALIAASLRANRGVNA
                     GLFHVERLLRRVDTFGFHLAALDVRLHASVLHEVIARGLDDPAWRTRGSRERRELLTA
                     ALEKDRGPAHELDALGKRSLAVFEAIVQGRHRYGGDALGYFIVSGASGADDVLAALLL
                     ARWASAYDKRTGEVALDIAPQFESEEALAACGATMQELLADPLYRHHLQARGRRQCVV
                     VGYSDSNKQAGACAARVMIHEAQRELARVLGSAGEQTVVFHARGSSIARGGGRIEALV
                     KAAPSGAVDGVLRIREQGATVKQGYGLRPIAMRTLERAFNALISA"
     gene            complement(2006..>2366)
                     /locus_tag="VM713_01265"
     CDS             complement(2006..>2366)
                     /locus_tag="VM713_01265"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVH82921.1"
                     /translation="GFIIDCQTQDLEAAARFWSAALGLEVRPPVQPDEAKYRTLATAP
                     DELHLEVQQVEHASRVHLDIEADDIAAEVARLERLGAKRIGPVRTWYVMEAPTGQRFC
                     VVRPQRADFAARANSWD"
BASE COUNT          327 a          843 c          841 g          355 t
ORIGIN      
        1 gcgctgatga gcgcgttgaa agcccgctcg agcgtgcgca tggcgatggg ccgcagtcca
       61 taaccctgct tcaccgtagc gccctgctcg cggatgcgca gcactccgtc cacggcgccg
      121 ctcggggccg ccttcaccag cgcctcgatg cgcccgccgc cgcgcgcgat gctgctgccg
      181 cgcgcgtgga acacgaccgt ctgctcgccc gcggagccga ggactcgcgc cagctcgcgc
      241 tgtgcctcgt ggatcatgac gcgcgcggcg caggcgccgg cctgcttgtt gctgtcggaa
      301 tagcccacca ccacgcactg gcggcgcccg cgcgcctgga ggtggtgccg atagagcgga
      361 tcggcgagca gctcctgcat ggtggcaccg cacgcggcca gcgcctcctc tgattcaaac
      421 tgcggggcga tgtcgagtgc cacctcgccg gtgcgcttgt cgtaggcaga ggcccagcgc
      481 gcgagcagca gcgccgccag cacgtcatcg gcgccgctgg ctccgctcac gatgaaatag
      541 ccgagcgcat cgccgccgta gcggtggcgc ccctgcacga tggcctcgaa cacggcgagc
      601 gagcgcttgc cgagcgcatc gagctcgtgc gccggaccac ggtccttctc cagcgccgcc
      661 gtgaggagct cgcggcgctc gcggctgccg cgggtgcgcc aggcgggatc gtcgaggcca
      721 cgcgcgatca cctcgtgcag cacgctcgcg tgcaggcgta catcgagggc ggcgaggtgg
      781 aagccgaagg tatcgacgcg ccgcaacagc cgctcgacgt ggaagagccc ggcgttgacg
      841 cccctgttgg ctctgaggct cgcggcgatc agcgcgatgt cggcgcgaaa ctgctgcggc
      901 ccctcgtagc cgtaggcgcg cccgtcgtag gcatggcgca gccgctcgcc aacctgcgcg
      961 agaaagaggc ggtagggcat gcgctcgtgg tgcatccgcg tggtggcgcg cgccgccggc
     1021 agcagcgccg tgtagcgctc gatgcgctgc gccagctcgg cggagatagc gacgcggctg
     1081 gcgctctgcg acaggcgctg cgcgaggctc tggcactcgg cgaagtagct gttgaccagc
     1141 acctgctgtt ggcgcgccag gctctcgcgg atggtcttgg cgtgcacgtc gggattgccg
     1201 tcggtgtcgc cgcccgccca gctgccgaaa tgcaggatgg ccggcacgcg caccacggct
     1261 gcctccggcc cgtagacctt ggcgagcgcc tgggcgatct cctcatagaa agccggcacc
     1321 acctggtaga ggatctcgag cagatagaac aggatctgct cgcgctcgtc cgcgaccgtg
     1381 agccgctcgc gcggcagctc ctcggtctgc cacgccgtgg tcatctccgt gcggatgctg
     1441 ctccagaggt cgcgctgctc gccgggcgtg agcgccgggt cgagccgctc gagcaggcgc
     1501 tccgcgatgc gctgctcctt gcgcagcagc gtgcggcggt tcgaatcggt ggaatgagcg
     1561 gcgaacaccg cctcgctgga caggctctgg atcagctcga gcacttcgcc gagtgcccag
     1621 ccctcctgcc tgagcccggc gatcgcatcc tcgacgccgc cgggttgcgg ccgctggctg
     1681 tccttcacca ggtagtcgcg gcgccggcgt atgcgatgga ctcgctccgc gagattcacc
     1741 gcctggaacc aggtggcgaa ggcgcgcacc aggtcgcgcg ccagtgccgg cggccggtcg
     1801 cgcacgtggg cggcgagctc cgcgcgcgcg tcactctcgc cggcgcggct gcggatcgcg
     1861 gcgacgcggt ccagctccac gagctcaaac agcggctcgc caccctgctc gcgcaggatc
     1921 tcgcccagca gcgcgccgag cgcatgcaca tcgtcgcgca gcggctcgtg cttgggggga
     1981 aagtggatgt cgttgcggtt caacatcagt cccagctgtt ggcgcgcgcc gcgaagtcag
     2041 cgcgctgcgg gcgcaccacg cagaaacgct gccccgtggg cgcctccatc acgtaccagg
     2101 tgcgcaccgg cccgatgcgt ttcgcgccga gccgctcgag ccgcgccact tcggccgcga
     2161 tgtcgtcggc ctcgatgtcg aggtgcacgc ggctcgcgtg ctcgacctgc tgcacctcga
     2221 gatgcagctc gtcgggcgcg gtggcgaggg tgcggtactt ggcctcatcg ggctgtaccg
     2281 gtggacgcac ctcgagaccg agcgccgcgc tccagaagcg tgcggcggcc tcaagatcct
     2341 gggtctgaca gtcgatgatg aatccc
//
LOCUS       DATLKL010000097         1809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_23626, whole genome shotgun sequence.
ACCESSION   DATLKL010000097 DATLKL010000000
VERSION     DATLKL010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23626"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VM713_01270"
     CDS             <1..1025
                     /locus_tag="VM713_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVH82922.1"
                     /translation="DVVSADHQNKPDVGSNIVRQWIDVDHVDAVVDTPTSSVALAVAQ
                     VVKEKNKAFLISGAASSDLTGKQCTPNNIHWTYDTWALAHGTGNAIVKQGGDTWFFIT
                     ADYAFGHALERDTSEVVKAAGGKVLGSVNVPLNTQDFSSFLLQAQASKAKIVGLANAG
                     GDTINSIKQAGEFGLVEGGQNLAGLLVFISDIHSLGLQTAQGLILTTAFYWDLNDKTR
                     AFAKRFAARNGGKYPTMVQAGVYAETLHYLEAVKKVGGAADGVKVVEAMKATEFDDPL
                     FGKTKVRADGTAVHDMYLVEVKKPSESKGPYDYYKIRATIPAAEAFRPLADEKSGMPD
                     YCPLVQ"
     gene            1115..>1809
                     /locus_tag="VM713_01275"
     CDS             1115..>1809
                     /locus_tag="VM713_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVH82923.1"
                     /translation="MFHLLGIPPQALFGQLLIGLINGSFYALLSLGLAVIFGLLNIIN
                     FAHGALYMTGAFCAWFLLTRLGLGYWWALFVSPIVIGALGIAIERSMLRWLYRLDHLY
                     GLLLTFGLALVIQGQFQNEFGSSGLPYQIPSSLTGGFNLGFMFLPTYRGWVVLASLAL
                     CLATWFMIERTKLGSYLRAATENPSLVQAFGVNVPRMVTLTYGFGVALAALAGVMAAP
                     IYQVSPLMGADLI"
BASE COUNT          303 a          598 c          569 g          339 t
ORIGIN      
        1 tcgacgtcgt ctcggccgat catcagaaca agccggacgt cgggtccaac atcgtgcggc
       61 agtggattga tgtcgatcac gtcgatgccg tcgtcgacac gccgacctcg agcgtggctc
      121 tcgcggtggc ccaagtggtg aaggagaaga acaaggcgtt cctcatctcg ggcgcggcga
      181 gctcggactt gaccggcaag cagtgcaccc ccaacaacat ccattggacc tacgacacct
      241 gggcgctcgc gcacggcacg ggcaacgcca tcgtcaagca gggcggcgat acctggttct
      301 tcatcaccgc cgattatgcc ttcggccacg ccctggagcg cgacacctca gaggtcgtga
      361 aagcggcggg gggcaaggtg ctgggctcgg tcaacgtgcc gctcaacacc caggatttct
      421 cgtccttcct gcttcaggcg caggcctcga aggccaagat cgtcgggctg gcgaatgccg
      481 gcggcgacac catcaactcg atcaagcagg ccggcgagtt cggcctcgtg gaaggcggcc
      541 agaacctcgc cggcctcctg gtcttcatct cggatattca ttcgctcggg ctccagaccg
      601 cgcaggggct gatcctcacg accgcctttt actgggacct caacgacaag acccgcgcct
      661 ttgccaagcg cttcgccgcg cgaaatggcg gcaagtaccc gaccatggtg caggccggcg
      721 tgtacgccga gacgctgcac tatctcgagg cggtgaagaa ggtaggcggc gccgccgatg
      781 gggtaaaggt agtcgaggcg atgaaggcga cggagtttga cgacccgctg ttcggcaaga
      841 ccaaggtgcg ggccgacggc acggctgtcc acgatatgta tctggtcgag gtgaagaagc
      901 cgtccgagtc gaaagggccc tacgattact acaagatccg cgccaccatc cccgcggccg
      961 aggcatttcg tccgcttgcg gacgagaaga gcggcatgcc ggattactgc ccgctagtcc
     1021 agtagggcca agacggcggg cggacgcacc gggcagcgcg gcgggcgggc gcccgctccc
     1081 cgcttctgac cgacgcgagg gagacgtcgc ttcgatgttc cacctgctcg ggatcccgcc
     1141 gcaagcgctc ttcggccagc tcctcatcgg gctgatcaac ggctcgttct atgcgttgtt
     1201 gagcctcggg ctggcggtca tcttcgggct gctcaacatc atcaatttcg cgcacggcgc
     1261 gctctacatg acgggcgcct tctgcgcctg gttcctcctg accaggctcg gcctgggcta
     1321 ttggtgggcg ctcttcgtct caccgatcgt gatcggagcg ctgggcatcg cgatcgagcg
     1381 cagcatgctg cgctggctct accgtctcga ccatctctat ggcctgctcc tgacattcgg
     1441 tctggcgctc gtcatccagg gtcagttcca gaatgagttc ggctcctcgg gtctccccta
     1501 ccagattccg tcttcgctca ccggcggatt caaccttggc ttcatgttcc taccgacata
     1561 ccggggctgg gtcgtgctgg cctcgctcgc gctttgcctt gccacctggt tcatgatcga
     1621 gcgcaccaag ctcggctcct acctgcgcgc cgcgacggag aacccgagcc tcgtgcaggc
     1681 cttcggcgtc aacgtgccgc gcatggtcac gctgacctat ggtttcggcg tggcgctcgc
     1741 ggcgctcgcc ggcgtgatgg cggccccgat ctatcaggtg agcccgctca tgggagccga
     1801 cctcatcat
//
LOCUS       DATLKL010000098         1577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_113872, whole genome shotgun sequence.
ACCESSION   DATLKL010000098 DATLKL010000000
VERSION     DATLKL010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113872"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1577
                     /gene="polA"
                     /locus_tag="VM713_01280"
     CDS             <1..>1577
                     /gene="polA"
                     /locus_tag="VM713_01280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HVH82924.1"
                     /translation="ARVLAALKPLFESAVHGKVGHNLKYDAHVLLNAGIALAGMRFDT
                     MLESYVWNSVATNHDMDSDARRYLGLTTITFEDVAGRGAKQLSFDQVPVEKAAEYAAE
                     DADVTLRLHQALWPQLTSVPPLARLYEECEQPLVPVLLAMEHHGVLVDREQLRAQSRE
                     FARELQQLLIEAHREAGHEFNIESPKQLQQILFERLQLPVRRKTPTGQPSTAEDVLEE
                     LAESYPLPRLVLEYRALAKLKSTYTDKLPEQVNERTGRIHTNYAQAVAATGRLSSVDP
                     NLQNIPIRRPEGRRIRQAFVAPPGCVLLAADYSQIELRIMAHLSGDESLRRAFAEDRD
                     VHQATAAEVFSVSSEAVTPDQRRTAKVINFGLIYGMSPFGLARNLGIERGAAQQYVER
                     YFQRYPGTRRFMDETRIQARERGYVETVFGRRLYLPDIRSGNTQLRQYAERSAINAPM
                     QGTAADIIKRAMIALDAWCRRPGSPARLIMQVHDELVLEVRAGAVEDVTRAVRERMVG
                     AATLSVPLRVDVGTGAN"
BASE COUNT          267 a          551 c          528 g          231 t
ORIGIN      
        1 cgcgcgggtg ctcgcggcgc tcaagccgct cttcgagagc gcggtccacg gcaaggtggg
       61 ccataacctc aagtacgacg cgcacgtgct gctcaacgca gggatcgcgc tcgccggcat
      121 gcgcttcgac acgatgctcg agtcgtacgt gtggaacagc gtcgccacca atcacgacat
      181 ggactcggac gccaggcgct acctcgggct caccaccatc accttcgagg atgtcgccgg
      241 caggggcgcg aagcagctct ccttcgacca ggtgcccgtg gagaaggccg cagagtacgc
      301 cgccgaggat gccgatgtga cgctgcgcct gcaccaggcg ctgtggccgc agctcacctc
      361 ggtgccgccg ctggcgcgcc tctatgagga gtgcgagcag ccgctcgtcc cggtgctgct
      421 tgcgatggag catcacggcg tgctcgtcga tcgcgagcag ctgcgcgcgc aaagccgcga
      481 gttcgcgcgc gagctgcagc agctgctaat cgaggcgcac cgcgaggcgg gccacgaatt
      541 caacatcgag tcgccgaagc agctgcagca gatcctgttc gagcggctgc agctgccggt
      601 gcggcgcaag acgcccactg gtcagccctc gacggccgag gatgtgctgg aagagctggc
      661 cgagagctac ccgctgccgc gcctggtcct cgagtaccgc gcgctcgcca agctcaaatc
      721 cacctacacc gataagctcc ccgagcaggt caacgagcgc accgggcgca ttcacaccaa
      781 ctatgcgcag gcggttgccg ccaccgggcg gctctcctcg gtcgatccca acctgcagaa
      841 catcccgatc cgccgtcccg agggacggcg catccgccag gccttcgtgg caccgccggg
      901 ctgcgtgctg ctcgcggcgg actattcgca gatcgagctg cgcatcatgg cgcacctctc
      961 cggggacgag agcctgcgcc gagcgttcgc cgaggatcgc gacgtacacc aggcaaccgc
     1021 cgcggaggtt ttcagcgtca gtagcgaagc ggtcacgccc gatcagcgcc gcaccgccaa
     1081 ggtgatcaat ttcggactca tctacggcat gtcgcccttc gggctggcgc gcaacctggg
     1141 catcgagcgt ggcgccgcgc agcagtacgt ggagcgctac ttccagcgct atcccggaac
     1201 caggcgcttc atggacgaga cgcgcatcca ggcgcgcgag cgcggctatg tcgagacggt
     1261 gttcgggcgg cgcctgtacc tgccggacat ccgctccggc aacacgcagc tgcgccagta
     1321 cgccgagcgc agcgccatca acgcgccgat gcagggcact gcggccgaca tcatcaagcg
     1381 cgccatgatc gcactggatg catggtgccg ccggcccggt tcccccgctc ggctcatcat
     1441 gcaggtgcac gatgagctgg tgctcgaggt gcgtgccggg gcggtcgagg acgtgacgcg
     1501 cgcggtgcgc gagcgcatgg tgggcgcggc aacgctctcg gtacccctgc gagtcgacgt
     1561 gggcacgggc gccaact
//
LOCUS       DATLKL010000099         4582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_23642, whole genome shotgun sequence.
ACCESSION   DATLKL010000099 DATLKL010000000
VERSION     DATLKL010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23642"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1286)
                     /locus_tag="VM713_01285"
     CDS             complement(108..1286)
                     /locus_tag="VM713_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVH82925.1"
                     /translation="MPDVPPSPTAAADPRVRRYVLIALAAALVLAAWGILSRLSARIA
                     LERQAAAAAIVTVATTHPSRGPESNLLVLPGTVQAFYEAPIYARTAGYLRAWYTDIGA
                     NVRRGQLLAEIDAPEVDQQLRQAQADLATAQANYELARTTNERWQGLLATESVSKQDA
                     DQRAGDAAAKAAATQSAAANLARLRELESFKRVVAPFEGVVTQRNTDVGALINAGETP
                     GSALFRVADTHRLRIYVSVPQSYAGEIHPGLAAGLVFADRPGKRYEAAVASTARALDA
                     SSRTLQVELQIDNRSGELLPGSYAEVQFDLSGGSSTLRIPVNAVLFRAQGLLVATVDS
                     SNRAHLHRITQGRDFGREIEVLSGVEPNDVLIVNPPDSISDGMQVRIAVPAAAPPGHG
                     "
     gene            complement(1279..4458)
                     /locus_tag="VM713_01290"
     CDS             complement(1279..4458)
                     /locus_tag="VM713_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH82926.1"
                     /translation="MWIVRLALRRPYTFIVLAIVILLLGVFAILRTPTDIFPNIRIPV
                     VAVIWRYTGLSPDEMATRLVLGSERNAQTTVNDVAHTESQSLSGTAVVKYFFQPGVKE
                     EMSFAQITGVSQTALRQAPPGTTPPFILAYNASSVPILQLALSSPSLSEAELFDLGNN
                     VIRTGLATVYGASMPYPYGGLQRQVQVDLDPDALRAQRLSGNDVTNAIASQNLILPAG
                     TEKIGDREYFVAVNASPKTIDELNNLPVTTRNGSVIYVRDVAHVRDGYPPQTNIVRRD
                     GHRGVLMSVLKTGAASTLDIIRSIKQRLPAIRAALPPDFHIELVGDQSLFVRAAIAGV
                     VREAVIAAALTALMILLFLGSWRSTLMIAISIPLSILASIVCLSALGETINIMTLGGL
                     ALAVGILVDDATVTIENINMHLEQGMDVEGAILTGAHEIALPALVSTLAICIVFVPMF
                     MLVGIARFLFIPLAEAVVFAMLASYLLSRTLVPTLAKYWLQRHDPHASASPQSPFAQL
                     QAGFALRFERLRASYRGLLERALAAGPRFALPFLGAMAATALLAFPFGRYLPGLGQDF
                     FPRVDAGQILLHLRGPTGIRIDETAALCDRVEATVRQVIPAAEIASLVDDIGVPYSGI
                     NLSYSTSAPVGPGDADIFVNLSAAHRPTDAYVRALRARLLLDYPGVTFAFLPADMVSQ
                     ILNFGLPAPLDVQISGFDVDGNRTYANALLARMHTIPGAVDLRIQQAFDYPQIDVVVD
                     RSKAALLGLTEQDVASNLLVSLSGSFQTSPSFWIDPKSGTQYSVAAQTPQYRLASLAD
                     LATTPITGTAGNPQLLANLASFHRSAGPAVVSHYDATPVIDIYGALEGTDLGFMAAQI
                     TKQIAAAGAELPRGSQVTVRGQVKTMTDSFNGLLLGLAGAVVLVYLLIVINFQSWADP
                     FIIITALPAALAGIVWMLFLTHTPVSVPALTGAIMCMGVATANSVLVVSFARERMNAG
                     DDAGHAALQAGVVRFRPVLMTALAMIIGMVPMALGLGEGGEQNAPLGRAVIGGLVFAT
                     VATLFFVPTVFTLIHGRLAVQPATGVADA"
BASE COUNT          774 a         1472 c         1648 g          688 t
ORIGIN      
        1 ctccttgtag cctgccgcag gcgaagcggt cgcgggacgc tgataacgcg gggcgagcga
       61 gcagccggcg agtgcgcagg cgatcgccac ggcgatcagg gtccgcatca accgtgtccg
      121 ggcggtgccg cggccgggac ggcgatacgc acctgcatac cgtcgctgat cgaatcgggc
      181 ggattgacga tcaggacgtc attgggctcg acaccgctca gcacctcgat ctcgcgaccg
      241 aagtcgcgcc cctgcgtgat gcgatgcagg tgagcgcggt tgctcgagtc gaccgtcgcg
      301 accagcagcc cctgggcgcg aaacaacacg gcattcaccg gaatccgcag cgtgctgctc
      361 ccgccgctca ggtcgaactg cacttccgcg taggagccgg gcagcagctc accgctccgg
      421 ttgtcgatct gcagctccac ctgcagagtg cgcgagctgg catcgagggc gcgcgcggtg
      481 gaggcgaccg ccgcctcgta gcgcttgccg gggcggtcgg cgaacacgag tcccgcggcc
      541 agcccggggt ggatctcgcc cgcgtagctc tgcggcaccg aaacgtagat gcgcaggcga
      601 tgcgtatcgg ccacgcgaaa cagggcgctg ccgggcgtct cgccggcatt gatcagcgcg
      661 ccgacatcgg tgttgcgctg tgtcacgacg ccctcgaagg gcgcgaccac gcgcttgaag
      721 gactcgagct ccctcaggcg tgcgaggttc gctgcggcgg actgggtcgc ggccgccttg
      781 gcggcggcgt ctccggcgcg ctggtccgcg tcctgcttgg agaccgattc ggtcgcgagg
      841 agtccctgcc agcgctcgtt ggtggtgcgc gcgagctcat agttggcctg cgcggtggcg
      901 aggtccgcct gcgcctggcg cagctgctga tcgacctcgg gggcgtcgat ctcggcgagc
      961 aactggcccc tcctcacgtt cgcgccgatg tccgtgtacc aggcgcgcag atagccggcg
     1021 gtgcgggcgt agatgggcgc ctcgtagaag gcctgcacgg taccaggaag caccagcagg
     1081 ttggactcag gtccgcggct cgggtgggtc gtcgccacgg tgacgatcgc cgccgccgcg
     1141 gcctgacgct cgagtgcaat gcgcgcgctc aggcggctca ggattcccca ggccgcgagc
     1201 accagcgccg cagcgagcgc gatcagcaca tagcgcctga cgcgcggatc cgccgcggca
     1261 gtgggggagg ggggcacgtc aggcatcggc gactcccgtg gcaggctgca cggcgagccg
     1321 tccatgaatc agcgtgaaga ccgtcggcac gaagaacaga gttgccacgg tcgcgaacac
     1381 caggccgccg atcacagcgc ggccgagcgg cgcgttctgc tcgccgccct caccgagccc
     1441 gagagccatg ggcaccatgc cgatgatcat cgcgagcgcc gtcatcagca ccgggcggaa
     1501 gcgcacgacc cccgcctgca gggccgcgtg ccccgcatcg tcgccggcgt tcatgcgctc
     1561 gcgcgcgaag ctcaccacca gcacgctgtt ggcggtcgcg acacccatgc acatgatggc
     1621 cccggtgagc gccggcacgc tcaccggggt gtgcgtgagg aacagcatcc acacgatccc
     1681 ggcgagcgcc gcaggaagcg ccgtgataat gatgaacgga tcggcccacg actggaaatt
     1741 gatcacgatc agcaggtaga cgagcaccac cgctcccgcg agcccgagca gcagcccgtt
     1801 gaaggagtcg gtcatggtct tcacctgtcc ccggaccgta acctgggagc cgcgcggcag
     1861 ctccgcgccg gccgcggcga tctgcttggt gatctgtgcc gccatgaagc cgagatcggt
     1921 gccctcgagg gcaccgtaga tgtcgatgac cggcgtcgcg tcgtagtggc tcactacggc
     1981 cggtcccgcg ctgcggtgga agctggcgag attggcgagc agttgcgggt taccggcagt
     2041 gccggtgatg ggcgtcgtgg cgagatcggc gagcgacgcc aaccggtact gcggagtctg
     2101 tgcggcgacc gagtactgcg tgccgctctt cggatcgatc cagaaggacg gcgacgtctg
     2161 gaagctcccc gagagtgaca cgagcagatt ggaggccacg tcctgctcgg tgaggccgag
     2221 gagtgccgcc ttgctgcgat cgacgaccac gtcgatctgc ggatagtcga aggcctgctg
     2281 gatgcgcagg tcgaccgcgc ccgggatggt gtgcatgcgg gcaaggagcg cgttcgcgta
     2341 ggtgcggttg ccgtcgacgt cgaagcccga aatctgcaca tcgagcggcg ccggcagacc
     2401 gaagttcaga atctgactca ccatgtccgc gggcaggaag gcgaacgtaa cccccgggta
     2461 atcgagcagc agtcgcgcgc gcagcgcgcg cacatacgcg tccgtgggcc ggtgagcggc
     2521 gctgaggttc acgaagatgt ccgcatctcc gggaccgacc ggcgcggacg tcgagtacga
     2581 gagattgatg ccactgtagg gaacgccgat gtcatcgacg aggctggcga tctccgcggc
     2641 agggattacc tggcgcacgg ttgcctcgac gcggtcgcac agcgccgcgg tctcatcgat
     2701 gcggatgccg gtcggcccgc gcagatgcag gaggatctgt ccggcatcga cgcgcggaaa
     2761 gaagtcctgc cccaggcccg gcaggtagcg gccgaaggga aaggcaagga gtgccgtggc
     2821 cgccatcgcc ccgaggaacg gcagggcaaa gcgtgggccg gcggcgaggg cgcgctcgag
     2881 cagcccgcgg tagctcgcgc gcagtcgctc gaagcgcaac gcaaagcccg cctgcagctg
     2941 cgcgaacggg ctctgcggac tcgcgctggc gtggggatcg tgccgttgca gccagtactt
     3001 cgccagggtc ggaaccagcg tccgcgacag gaggtaggag gccagcatcg cgaacaccac
     3061 cgcttccgcg agcgggatga agaggaagcg ggcgatcccg accagcatga acatcggcac
     3121 gaacacgatg cagattgcga gcgtcgagac gagtgccggc agggcgatct cgtgcgcgcc
     3181 ggtcagaatg gctccctcca cgtccatgcc ctgctcgagg tgcatgttga tgttctcgat
     3241 cgtgacggtg gcgtcatcga ccagaatgcc gaccgcgagc gcgagtccgc cgagggtcat
     3301 gatgttgatg gtctcgccga gggccgacag gcacacgatc gaggcgagga tcgacagcgg
     3361 gatcgagatg gcgatcatga gcgtgctgcg ccagctgccg aggaacagca ggatcatgag
     3421 cgcggtgagc gccgccgcga tcaccgcctc gcgcaccact ccggcgatgg cggcgcgcac
     3481 gaacagcgac tggtcgccca ccagttcgat gtggaagtcg ggagggagcg cggcgcggat
     3541 ggccggcagc cgctgcttga tgctgcggat gatgtcgagc gtcgaggcgg cgccggtttt
     3601 cagtacgctc atcagcacgc cacggtgccc gtcgcggcgc acgatgttgg tctgcggcgg
     3661 gtaaccgtcg cgcacgtgcg ccacgtcgcg cacgtagatc acgctgccgt tgcgggttgt
     3721 gaccggcagg ttgttgagct cgtcgatcgt tttcgggctg gcgttgaccg caacgaagta
     3781 ttcgcggtcg ccgatcttct cggttccggc cggcaggatc aggttctggc tggcgatcgc
     3841 attggtgaca tcgttaccgg agagcctctg ggcgcgcagc gcatccggat cgagatccac
     3901 ctggacctgg cgctgcagtc cgccgtaggg gtagggcatc gaggcgccgt ataccgtggc
     3961 gagccccgtg cggatgacgt tgttgccgag gtcgaagagc tcggcctccg aaaggctggg
     4021 gctggagagt gccagctgca ggatcggtac cgaggaggcg ttgtaggcga ggatgaacgg
     4081 cggggtggtg cccggcggcg cctggcgcag cgccgtctgc gacaccccgg tgatctgcgc
     4141 gaacgacatc tcctccttga cgcccggctg gaagaagtac ttgaccactg ccgtgccgga
     4201 cagcgactgc gactcggtgt gcgcgacgtc gtttacggtg gtctgggcgt tgcgctcgga
     4261 accgagtacc aggcgggtgg ccatctcgtc cggcgagaga cccgtgtagc gccagatcac
     4321 ggcgactacc ggaatcctga tgtttgggaa gatgtcggtg ggcgtgcgca ggatcgcgaa
     4381 cacccccaac aggaggatca caatcgcgag aacgatgaat gtataggggc gccggagcgc
     4441 cagtcggaca atccacatcg acccgccaca ctacctgagg gaggcgcggc ggcccatccc
     4501 cccggggtaa gacccggccg cctgccctaa ctcattgatc gtgccctgcg gcgcaccccg
     4561 ccgcagctcc attcagccgg tc
//
LOCUS       DATLKL010000100         4063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_79967, whole genome shotgun sequence.
ACCESSION   DATLKL010000100 DATLKL010000000
VERSION     DATLKL010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79967"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="VM713_01295"
     CDS             complement(<1..1387)
                     /locus_tag="VM713_01295"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HVH82927.1"
                     /translation="MYPTFMKPAATRSTMIVAFLTLAGAAAGADPLVHPQEWPRPASA
                     LAPDAQLEARIDALLARMTPAEKVGQLIQADIGSITADDLRHYLLGSVLNGADVAPGN
                     DKLASPREWLRLADRFYEASTAAPPALPVLWGTDAVHGDNNIPGATIFPHNIGLGAAR
                     DPALLRRIGAITALEVRVTGLDWAFAPSVAVARDARWGRTYESYGEDPRLVAECGAAL
                     VEGLQGRPGTGEFLDSAHVVATAKHFLGDGGTGGHDEGDNTASERELRDVDAAGYVAA
                     IAAGVQTVMASFSSWRGVKMHGDRALLSEVLKERMGFDGLVTGDWDGHAQVPGCSSTS
                     CAPAVNAGIDVLMAPESWGDLFANLLAQVNSAEIPRARLEDALRRVLRVKLRAHLPEE
                     GRPSSRPYAGHLELLGSPAHRAVARQAVRESLVLLKNDKQLLPLSARSRVLVAGDGAD
                     NVRKQCGGWTID"
     gene            1405..2022
                     /locus_tag="VM713_01300"
     CDS             1405..2022
                     /locus_tag="VM713_01300"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HVH82928.1"
                     /translation="MLDPVTELRNSLPASATAHGPPDLLLIERARAADAAAVGTLLRR
                     YAQRLFRIARSALGDEQAAESAVAETCVAAFEELRRHEPAGKFAVWLTRMALEQVRAH
                     RERQPAARPAPLYRHPLEPAIDALPEVFRTVFVLRVVEGISGTETAAALGLNVTTVRT
                     RLYRSHRRLSGGATLHASQGVFELDGARAERIVGQVLQRLQARDA"
     gene            2167..>4063
                     /locus_tag="VM713_01305"
     CDS             2167..>4063
                     /locus_tag="VM713_01305"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HVH82929.1"
                     /translation="MAQADPLSAYLPLNLEPEMERQIERVLILADEPILKRPFPVELV
                     KAALPDACKKDAALCAKVRRYLERYSHDYALTHASASGAATRGAHVVVPNQHGLTEND
                     DWQVSAQAYVQPSDYLLASLGGIAYSGRTQATGSMVSFGASWAQLDAGYRDHWLSPMT
                     DTGSMLFSTESPTMPSVTLSNWEPLTRLGIQYEFILGKLSQTGANSGADLPGNNILYN
                     GVSSRGDPHLFSTQLSIEPFPGWSLGFNRNLEYGGGSGLPSSASFLLRDFFKPSGLSQ
                     TQGNQEASYVSRFIFPGKTPFAVYFQYSGEDNSDGGSYLLGNAALSAGIDFPRIGRHF
                     DATYEVAEWQNIWYVHYIFLDGMANYGDVLGTWGGDQRNFGSGVGARSQMLRVGWEPP
                     FGGYLEARARTLANQNYYAAGAQRQFAPGEPPPYPYFHYYDASLTYSRPWNGVTLGAQ
                     ATVGRDVYGNSFTRFAAFVRYGGEARSHADDSAPEDGDAEDAQPDTHGAELFVDAGVN
                     GNQVRVDLDGVAPITKSKLAFGPHFGLGARRAVSSWSDLGARLELDRVDGHSLLGARV
                     VDYRHRFGDAFALSLFAGVDRYDLATPAYSVYGGLGVQWRNFLRALPGWDLGLDFRYG
                     QNIARDHV"
BASE COUNT          615 a         1449 c         1372 g          627 t
ORIGIN      
        1 agtcgatcgt ccagccgccg cattgcttgc gcacattgtc ggcgccgtcg cctgcgacca
       61 gcacgcgcga gcgggcgctg agcggcagga gctgcttgtc gttcttgagg agcacgagtg
      121 actcgcgcac cgcctgccgc gccacggcac ggtgcgcggg cgatccaagg agctcgaggt
      181 gcccggcata ggggcgcgag gaaggccgcc cctcctcggg gagatgcgcg cgcagcttca
      241 cgcgcaggac gcggcgcagt gcgtcctcga gccgcgccct cggaatctcc gcggaattca
      301 cctgcgcgag caggttcgcg aacagatcgc cccagctctc cggcgccatg agcacgtcga
      361 tcccggcatt gacggcgggt gcgcaactgg tgctgctgca gccaggcacc tgcgcgtgcc
      421 cgtcccagtc gccggtcacg agcccgtcga agcccatccg ctccttgagc acctcggaga
      481 gcagggcacg gtcgccgtgc atcttcacgc cccgccagct cgagaacgag gccatcacgg
      541 tctgcacgcc ggcggcgatc gcggccacgt aaccggcggc gtcgacgtcg cgcagctccc
      601 gctcgctcgc ggtgttgtcg ccctcgtcgt gcccgccggt gccgccgtcg ccgaggaagt
      661 gcttcgcggt cgccaccacg tgcgcggagt cgaggaactc ccccgttccc gggcggccct
      721 gcaagccctc gacgagtgcg gcgccgcatt cggccacgag gcgcggatcc tccccgtagc
      781 tctcgtaggt acggccccag cgcgcgtccc gcgccaccgc cacactcggc gcgaacgccc
      841 agtcgagccc ggtgacgcgc acctcgagcg ccgtgatcgc gccgatgcgg cgcagcagcg
      901 ccggatcgcg ggcggccccg aggccgatgt tatgcggaaa gatggtggcg cccggtatgt
      961 tgttgtcccc atgcaccgcg tccgtccccc acaggaccgg cagcgcgggc ggcgcggccg
     1021 tggatgcctc gtagaaccga tcggccagcc tcagccactc acgcggtgaa gccagcttat
     1081 cgttgccggg cgcgacgtcc gcaccgttca gcaccgagcc gaggagatag tgccgcaggt
     1141 cgtccgcggt gatgctgccg atgtcagcct gaatgagctg accaaccttc tccgccggcg
     1201 tcatgcgcgc aagcagcgcg tcgatgcgcg cttcgagctg cgcgtcgggc gccaacgcgg
     1261 acgcaggacg gggccactcc tgcgggtgca cgagcgggtc ggcgccggct gccgcgccgg
     1321 ccagcgtcag gaaggcgacg atcatcgtgc tgcgcgtcgc cgcgggtttc atgaacgtag
     1381 gatacacaca cgaggttcgc atccatgctc gatccggtga ccgaactaag aaacagcctt
     1441 cccgccagcg ccactgcgca tggcccgccc gacctgctgc tcatcgagcg ggcgcgcgcc
     1501 gccgatgcgg ccgcggtcgg cacgctgctg cgccgctacg cgcagcggct gttccgaatc
     1561 gcgcgcagcg cgctcggcga cgagcaggcc gcggagagcg cggttgcgga gacctgcgtc
     1621 gccgcgttcg aggagctgcg gcgccacgag ccggcgggaa agttcgccgt gtggctcacc
     1681 cgcatggcgc tcgagcaggt gcgggcgcac cgcgagaggc agccggcggc gcgcccggcg
     1741 ccgctttatc gccatcccct cgagcctgcg atcgatgcgc tcccggaagt gttccgcacg
     1801 gtgttcgtgc tgcgtgtggt cgagggcatc agcggcaccg agactgcagc cgccctcggg
     1861 ctcaacgtca ccacggtacg cacgcggctc tatcgctcgc accggcgcct gagcggcggg
     1921 gcgacgctgc atgcatcgca gggcgtgttc gagctcgacg gcgcacgcgc cgagcgcatc
     1981 gtcgggcagg tgctccagcg actgcaggcg cgcgacgctt gacggccacg cgcgcgctgt
     2041 gtgtactccg atccgggcgc cgcggtgccc cggatggtca ggagtcgact cggcatcgtg
     2101 cgctcatggt tcagacgact caggcgcagg ggagcgccgc tgtgcgccgc gtgcgcgctg
     2161 gctgcagtgg cccaggcgga cccgctgagt gcctacctgc ccctcaatct cgagcccgag
     2221 atggagcggc agatcgagcg ggtgctgatc ctcgccgacg agccgatcct taagcgcccc
     2281 tttcccgtcg agctggtcaa ggcggctctc ccggacgcct gtaagaagga cgccgcgctc
     2341 tgcgccaaag tgcgccgcta cctcgagcgc tactcgcacg actacgcact gacgcacgcg
     2401 agcgccagcg gcgccgccac caggggcgcg cacgtcgtcg tgcccaatca gcacgggctc
     2461 accgagaacg acgactggca ggtctcggcg caggcctacg tgcagccgag cgactatctg
     2521 ctggcgagcc tcgggggcat cgcctactcg ggccgcacgc aggccacggg ctcgatggtg
     2581 agcttcgggg caagctgggc gcagctcgac gccggctatc gcgatcattg gctctcgccc
     2641 atgaccgaca cgggcagcat gctcttcagt accgagagtc ccaccatgcc atcggtgaca
     2701 ctgtcgaact gggagcccct cacccggctc ggtattcagt atgaattcat tctcggcaag
     2761 ctgtcgcaga ccggtgccaa cagcggcgcg gaccttcccg gcaacaacat tctctacaac
     2821 ggcgtctcga gccgcggcga cccacacctc ttcagcacgc agctgtcgat cgagcctttc
     2881 ccgggctggt cgctcggctt caaccgcaac ctcgagtacg gcggcggcag cggcctgccg
     2941 agctcggcga gcttcctgct gcgggatttc ttcaagccga gcggactctc gcagacgcag
     3001 gggaaccagg aagcctccta cgtcagccgc ttcattttcc ccggcaagac accctttgcg
     3061 gtgtatttcc agtactcggg cgaagacaac tccgacggcg gcagctacct cctcggcaac
     3121 gccgcgctct cagccggcat cgattttcct cgcattgggc ggcacttcga cgcgacctat
     3181 gaggtcgcgg agtggcagaa catctggtac gtgcactaca tcttcctcga cggcatggcc
     3241 aactacggtg acgtgctcgg cacctggggc ggcgatcagc gcaacttcgg cagcggcgtc
     3301 ggcgcgcgca gtcagatgct gcgcgtcggg tgggagccgc ccttcggcgg ctatctcgag
     3361 gcccgggcgc gcacgcttgc caatcagaac tactatgccg caggagcgca gcggcaattc
     3421 gccccgggcg agccaccgcc gtatccctac ttccactatt acgatgccag tctgacctat
     3481 tcgcggccgt ggaacggcgt cacgctcgga gcccaggcga ccgtcggccg agacgtctat
     3541 ggcaactcat tcacgcgttt tgcggccttc gtgcgctacg gaggggaagc gcgctcgcac
     3601 gcggacgatt cagcgccgga ggacggggac gcggaagatg cccagccgga cacgcacggc
     3661 gccgagttgt tcgtcgatgc gggtgtgaat gggaaccagg tgcgcgtcga tctcgacggc
     3721 gtcgccccga tcaccaagtc caagctcgcc ttcggcccgc acttcgggct cggcgcgcgg
     3781 cgcgcagtct ccagctggag cgatctcggg gcgcgcctcg agctcgatcg ggtcgatggc
     3841 cactcgctgc tcggtgcgcg cgtggtcgat taccgacacc gcttcggcga tgcgttcgcg
     3901 ctcagtctct ttgccggggt ggaccggtat gacctggcga cccccgccta ctcggtgtac
     3961 ggaggcctgg gcgtccagtg gcgcaatttc ctgcgggcgc tccccgggtg ggacctggga
     4021 ctcgacttcc gctatgggca gaacatcgcc cgcgaccacg tgc
//
LOCUS       DATLKL010000101         1627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_102604, whole genome shotgun sequence.
ACCESSION   DATLKL010000101 DATLKL010000000
VERSION     DATLKL010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102604"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VM713_01310"
     CDS             complement(<1..446)
                     /locus_tag="VM713_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82930.1"
                     /translation="MDARAFAALPDPFPMWRGGVLHGGRIAYETWGRLNAARDNALLL
                     FTGLSPPAHAASSERDPSDGWWQGMVGAKLAIDTDRYFVICVNSLGSCFGSSGPASPD
                     PATGQPYRLSFPDLSVEDIARGGYETVRSLGIERLDTVMGPSLGGMV"
     gene            complement(514..1455)
                     /gene="cysK"
                     /locus_tag="VM713_01315"
     CDS             complement(514..1455)
                     /gene="cysK"
                     /locus_tag="VM713_01315"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HVH82931.1"
                     /translation="MIYESILGTIGKTPIVRVQRLGPQHVTLYVKCEFFNPLASVKDR
                     LAIAIIEDAERTGTLKPGQTVVEATSGNTGIALAMVCAAKGYPFVAVMSETFSVERRR
                     LMKMLGAKVILTPAAERGSGMVRKAEELAKKHGWFVARQFENEANPAYHRNTTGPEIL
                     QDFVGKRLDYFVTGWGTGGTLTGAGQMIRLARPEVQIVACEPQGAALLSGKPWAPHKI
                     QGWTPDFVPEVLRREVAHRIVTVADTEAVETARALARSEGILCGISSGGTFAAALKIA
                     AEASEGAVILAMLPDTGERYLSTVLFEGISDGGDPEP"
BASE COUNT          265 a          546 c          553 g          263 t
ORIGIN      
        1 accatgccac caagagaggg ccccatgacg gtatccagcc gctcgatgcc tagcgagcgc
       61 accgtctcgt agccgccgcg cgcgatgtct tccaccgaaa ggtccgggaa gctcagccgg
      121 tagggctgcc ccgtggccgg atccggcgag gcgggccccg aggagccgaa gcagctgccg
      181 agcgagttga cgcagatgac gaagtagcga tcggtgtcga tcgcgagctt tgcgcccacc
      241 atcccctgcc accagccatc cgacggatcc cgctccgagg aggcggcatg agcggggggc
      301 gagagcccgg tgaataaaag cagcgcgttg tcgcgcgccg cgttcagcct cccccaggtc
      361 tcataggcga tgcgcccgcc gtgcagcacg ccgccgcgcc acatcgggaa cggatcaggc
      421 agggcggcga atgcgcgcgc atccatgggc caagaataga gcacgcggcg gtgttcccaa
      481 gcgcccgacg tccctggcgg cttggttccg ccttcagggc tccgggtcgc ccccgtcgct
      541 aatgccctcg aagagcacgg tcgacaggta gcgctcgccg gtgtcgggca gcatggcgag
      601 gatcaccgcg ccctcgctcg cctcggcagc gatcttgagc gcggcggcga atgtgccacc
      661 ggaggagatt ccgcagagaa tcccctcgct gcgcgcgagc gcccgcgccg tctccaccgc
      721 ctcggtatcg gcgacggtca cgatgcgatg ggcgacctcg cgcctgagga cctcgggaac
      781 gaagtccggc gtccagccct ggatcttgtg cggcgcccag ggcttgcccg agaggagcgc
      841 cgccccctgc ggctcacagg caacgatctg cacctccgga cgggcgaggc ggatcatctg
      901 ccccgctccg gtgagcgttc ctcccgtgcc ccagccggtg acgaagtagt cgagccgctt
      961 gccgacgaag tcctgcagga tctcaggtcc cgtcgtgttg cgatggtagg ccgggttggc
     1021 ttcgttctcg aactgccgcg cgacgaacca gccgtgcttc ttcgcgagct cctcggcctt
     1081 tctgaccatt cccgagccac gctccgcagc cggggtgagg atgaccttgg cgcccagcat
     1141 tttcatcagc cgccgccgct cgacggaaaa ggtctcgctc atcacggcaa cgaacggata
     1201 gcccttggct gcgcatacca tggcgagcgc gatgcccgtg ttgccggagg tggcttccac
     1261 cacggtctgg ccgggtttga gcgtgcccgt gcgctcggca tcctcgatga tggcgatcgc
     1321 gaggcggtcc ttgaccgagg cgagcggatt gaaaaactcg cacttcacgt acagggtgac
     1381 gtgctgcggc ccgagccgct ggacgcggac gatgggtgtc ttgccgatcg tgccgaggat
     1441 gctttcgtag atcatgttgt ccttctaacc tcagggccat cataccggca agaaataccg
     1501 gggtatcacc ttagggcagg ctgttatgag gcgcgagggt acaggggtga ggctgcagaa
     1561 gcttctcgcc gacgcaggcc tcgcctcgcg gcgcgcggcg gaggactgga ttcgcgcggg
     1621 gcgcgtg
//
LOCUS       DATLKL010000102         2916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_147702, whole genome shotgun sequence.
ACCESSION   DATLKL010000102 DATLKL010000000
VERSION     DATLKL010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147702"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..794
                     /locus_tag="VM713_01320"
     CDS             171..794
                     /locus_tag="VM713_01320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVH82932.1"
                     /translation="MNIDAAVWAVLADYEARAVREEALWDTLGPEEAERRIDEMLLPV
                     GRATGTLLNLLAKEACARRILEIGSSYGYSTVWLAEAARAVHGKVISLELKADKTEYA
                     TAQLARAGLADFVEFRIGDALASLAALPGPFDLVLIDLWKNLYVPVFELLHPKLAPGA
                     LVVADNMIEPASARPHAQAYRHAVHVARDMSTVLLPVGNGIELSRYQ"
     gene            complement(778..1497)
                     /locus_tag="VM713_01325"
     CDS             complement(778..1497)
                     /locus_tag="VM713_01325"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HVH82933.1"
                     /translation="MSTELLFRDDAYLKTAEARVLVAGARGIELDRTVFYPLGGGQAG
                     DTGALVRTSGERIPIADTRKGDTPDGVLHIPASGAPLPEPGETVGLEIDWPRRYRLMR
                     LHTALHLLSCVIVAPVTGGNIVPDKARLDFDIELAALDAVRIESETNRLIALGSATET
                     AWITDAELDAQPELVKTMSVQPPRGLGRVRLLRIPGIDLQPCGGTHVANIAEIGAIRV
                     MKIRSEGRHNKRVEIALTDSG"
     gene            1531..2373
                     /locus_tag="VM713_01330"
     CDS             1531..2373
                     /locus_tag="VM713_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HVH82934.1"
                     /translation="MAAFSYRIVNVFTQGRVALSGNPLCVFERAQALDAPRMQALALQ
                     FNLSETSFILPSERANAAARIFTPRYEMPFAGHPTLGTAHVCRALGLGGDRLTLELPA
                     GLIPVRATGDRWTLQARAPVWREVSEPLLTLAAMLGLEERDLGERPLWVNAGVEQLIV
                     PLASEAAVRRVNVRADLLAQLRSADGHSQVYVFASAGAGLLARFFFPQGRAVLEDPAT
                     GSACANLGGWCLATSRTLPCEFDISQGEYTGRPSALRLRVDEERNVLVSGDVIELGRG
                     TLNL"
     gene            2394..>2916
                     /locus_tag="VM713_01335"
     CDS             2394..>2916
                     /locus_tag="VM713_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVH82935.1"
                     /translation="MRLVSLAAAAALLASFATFAAPAPTPSMQPDIPAEFTAPTTAFD
                     YVKREIMIAMRDGVKLHTVIVIPKGAQGAPIILTRTPYEAANATEHTPSPHILATLPE
                     ADDVFVSRGYIRVYQDVRGKYGSEGDYVMTRFARGPLNGTTVDHSTDTYDTIEWLVKN
                     VPESNGKVGMIGCS"
BASE COUNT          436 a         1011 c          984 g          485 t
ORIGIN      
        1 tcggctgcgc tcgggctcgc ttaccaggtg agccggctgc cgttcgccgc gcttctcgac
       61 gagcaaggtg tggtgcgcgc acgcggactg gtcaactctc gagaacacct cgagagcctg
      121 ttcgaggcca agcggctcgg cgtggcctct ctccaggagt acttctcatc atgaacatcg
      181 atgccgctgt ctgggccgtg ctcgccgact acgaggcgcg ggccgtgcgc gaggaagctc
      241 tctgggacac cctcgggccc gaggaggccg agcggcgcat cgacgagatg ctgctgccgg
      301 tcggacgcgc gaccggcacc ctgctcaatc tgctcgccaa ggaggcctgc gcgcgccgta
      361 tcctcgagat cggcagctcc tacggctatt cgaccgtctg gctcgccgaa gcggcgcgcg
      421 cggtccacgg caaggtgatc tccctcgagc tcaaagccga caagaccgag tacgcaaccg
      481 cccagctcgc gcgcgccgga cttgccgact tcgtcgagtt tcgcatcggc gatgcgctcg
      541 cgagcctcgc cgcgcttccc gggcccttcg atctggtgct catcgacctg tggaagaacc
      601 tctacgtgcc ggtgttcgag ctgctgcacc cgaagctcgc gccgggcgcg ctcgtggtcg
      661 ccgacaatat gatcgagccg gcgagcgcac gcccgcacgc gcaggcctat cgccacgcgg
      721 tgcacgtcgc gcgcgacatg agcaccgtgc tgctcccggt cggcaacggc atcgagctca
      781 gccgctatca gtgagcgcga tctccacgcg cttgttgtgc cgcccctcgc tgcggatctt
      841 catgacgcgg atcgcgccga tctcggcgat gttggccacg tgggtgccac cgcagggctg
      901 caggtcgatc cccggaatcc tgagcagccg cacccggccg aggccgcgcg gcggctgcac
      961 gctcatggtc ttcaccagct ccggctgcgc gtcgagctcg gcatccgtga tccaggcggt
     1021 ttccgtggcg cttcccaggg cgatcagccg gttggtctcg ctctcgatgc gcaccgcatc
     1081 aagcgcggcg agctcgatgt cgaagtcgag gcgcgccttg tcgggcacga tgttgccacc
     1141 ggtcacgggg gcgacgatca cgcacgacag gaggtgcagg gcggtgtgca gccgcatcag
     1201 ccggtagcgc cgcggccagt cgatctcgag accgaccgtc tcgccgggct cggggagtgg
     1261 cgctccgctc gccgggatgt gcagcacgcc gtccggcgtg tcgcccttgc gcgtatccgc
     1321 gatcgggatg cgctcgccgc tcgtgcgcac cagggctccg gtgtcgccgg cctgtccgcc
     1381 accgagcgga taaaacaccg tacggtcgag ctcgatgccg cgcgcacccg ccaccagcac
     1441 gcgcgcctcg gcggttttca ggtaggcatc gtcgcgaaac agcaattcgg tgctcatcga
     1501 tgcggcctcg aggatacaat cgtcgcgcgc atggctgcgt tctcctaccg gatcgtcaac
     1561 gtgttcaccc agggtcgcgt tgccttgagc ggcaaccccc tgtgcgtgtt cgagcgcgcg
     1621 caggcgctcg atgcgccaag aatgcaggca ctggcactgc agttcaatct gtcggagacc
     1681 agcttcatcc tgccttccga gcgcgccaac gccgcggcgc ggatcttcac gccgcgctac
     1741 gagatgccct tcgccggaca cccgacgctc ggcacggcgc acgtctgccg ggccctgggg
     1801 ctcggcggtg accgcctgac gctcgagctg cccgcggggt tgatcccggt gcgcgccacg
     1861 ggcgaccgct ggacgctgca agcgcgggcg ccggtgtggc gcgaggtcag cgagccgctg
     1921 ctcacgctcg ctgccatgct cgggctcgag gagcgcgacc ttggcgagcg gcccctgtgg
     1981 gtgaatgcgg gcgtcgagca gctcatcgtg ccgctggcga gcgaggcggc ggtgcggcgc
     2041 gtgaacgtgc gcgcggacct cctcgcacag ctgcgcagcg ccgacggcca cagtcaggtg
     2101 tacgtgttcg cgagcgcggg tgcgggactg ctcgcgcgct tcttttttcc gcaggggcgg
     2161 gcggtgctcg aggatccggc gaccgggtcc gcctgtgcga acctcggcgg ctggtgcctc
     2221 gccacgagcc gcacgctgcc gtgcgagttc gacatctcac agggtgaata caccggcagg
     2281 ccgtcggcac tgcggttgcg ggtcgacgag gagcgcaacg tactggtgag cggcgatgtc
     2341 atcgagctcg ggcgcggcac gctcaacctg tgaatgaaag gccggtaatt ctcatgcgcc
     2401 tcgtttcact tgccgccgcc gcggcgttgc tagcctcttt cgccacgttc gcagctccgg
     2461 cgccgacgcc gtccatgcag ccggatattc ccgccgagtt caccgccccg acgaccgctt
     2521 tcgactacgt caagcgcgag atcatgatcg cgatgcgcga cggcgtgaag ctgcataccg
     2581 tcatcgtcat cccaaagggc gcgcagggcg cgccgatcat cctgactcgt acgccctacg
     2641 aggccgcgaa cgccaccgaa cacacgccga gcccgcacat cctggcgact ctgcctgagg
     2701 cggatgacgt gttcgtctcc cggggatata tccgcgtata tcaggacgtg cgcggcaagt
     2761 acggttcgga aggcgattac gtcatgacgc gcttcgcgcg cggaccgctg aacggcacga
     2821 cggtcgatca ctcgaccgac acctacgaca cgatcgagtg gctggtgaag aacgtgcccg
     2881 agtcgaacgg caaggtgggg atgatcggct gttcct
//
LOCUS       DATLKL010000103         3155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_1148, whole genome shotgun sequence.
ACCESSION   DATLKL010000103 DATLKL010000000
VERSION     DATLKL010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1419)
                     /locus_tag="VM713_01340"
     CDS             complement(7..1419)
                     /locus_tag="VM713_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004148125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HVH82936.1"
                     /translation="MSVASAPAGLPAVIEVRSIDYIPDAERHGGLASQFTLWLAANLQ
                     ITAIVTGALAVVFGGDVFWSLIGLLLGQLIGGAVMALHAAQGPQLGLPQMISSRVQFG
                     VYGAAIPIVAVCLMYIGFSASGCVLAGQAIGRLLDVDDAVGIVVFTCFIVVVTIFGYR
                     VIHVLGRIAAVIGVLAFAYMFARLFATHDVSALLAQRHFSLSQFLLAMSLSASWQIAY
                     GPYVADYSRYLPRDTSVAKVFAAVGLGSVIGSQISMTFGVFAAAVAGKQFEHHEVAYI
                     VGLAAAGVVSSLPYFSIAFGKVTVTALNAYGSFMSMATIVSGLRRGVRISVRTRFFYI
                     VAMVGVSALLALTGRHSFLKAFEAFILCLLAVFTPWSAINLIDYYCFTRSRYDVPALS
                     DPDGRYGRWNATGIVIYLIGILVQIPFLSTAFYTGPLVEPLGGVDISWIIGLIVPGIL
                     YYALGRASQRRAPPQIILPA"
     gene            complement(1849..3078)
                     /locus_tag="VM713_01345"
     CDS             complement(1849..3078)
                     /locus_tag="VM713_01345"
                     /inference="COORDINATES: protein motif:HMM:NF004788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate carboxypeptidase"
                     /protein_id="HVH82937.1"
                     /translation="MFGHLISIIAIAAAMTAPAVTRAATEKNARVLSAAHASRTAELQ
                     LLEQVVNIDSGTGDVEGGRKVAAVLIPRLKALGMTLETVPAEEAGLAENTVATLAGRG
                     KRRILMIGHIDTVFEPGTAQQRPFRTDDKRAYGPGVSDEKGGVVEGLYALQILHDLGF
                     TNFGKIVFLMETSEERGSPGTRALIGRLLKDADVELNLEPGDFPDTLTVWRKGSDTFR
                     IEVKGRAAHAGIAPQDGRNAALELIHQLSGLEVFPKSGDGLTVNLTVMHAGTRGNIIP
                     EDAWALANVRVRDKADFERVAGVLRKNAETTVIPDTRVTVTHGEGFPPLPSNPATDAL
                     AARAQRIYAGIGRTLIPGGNGGASESALAADARVAALDGLGPVGGGFHSDKEFLELDS
                     VTPRLYLLTMLLMELAK"
BASE COUNT          600 a         1043 c         1042 g          470 t
ORIGIN      
        1 ggtcacttac gcgggcagga tgatctgtgg cggcgcgcgc cgctgcgagg cccgcccgag
       61 cgcgtaataa agaatgccag gcacgatcag accgatgatc caggagatgt cgacgcctcc
      121 gaggggctcc accagcggac cggtatagaa ggcggtcgat aggaacggga tctgcaccag
      181 gatgccaatc aggtagatca cgatgccggt ggcattccag cgcccgtagc gcccgtcggg
      241 atcggataac gccggcacgt cgtagcgcga gcgcgtgaag caatagtagt cgatgagatt
      301 gatcgcgctc cacggcgtga acaccgcgag cagacacaga atgaaggcct cgaaggcctt
      361 gagaaacgag tgacgtccgg tcaatgcaag cagcgccgag acaccgacca tcgcgacgat
      421 atagaagaag cgagttcgca ccgaaatgcg cacgccgcgc cgcaggccgc tcacgatcgt
      481 cgccatggac atgaagctcc cgtaggcgtt gagcgccgtg accgtgacct tgccgaacgc
      541 gatgctgaag tacgggagac tcgaaaccac cccggccgcg gcgagtccga cgatgtaagc
      601 cacctcgtga tgctcgaatt gtttgccggc caccgcggcg gcaaacacgc cgaaggtcat
      661 ggatatctgc gacccgatga ccgagcccag gccgacggcg gcgaatacct tcgcgacgga
      721 ggtgtcgcgc ggcagatagc gcgagtaatc ggcgacatac ggaccgtagg cgatctgcca
      781 ggaggccgag agcgacatcg ccagcagaaa ctggctcagc gaaaagtgcc gctgcgccag
      841 caaggcagac acgtcgtgcg tggcgaacag ccgggcgaac atgtaggcaa aggcgagcac
      901 gccgatcacg gccgcgatcc gcccgagcac gtgaatgact cgatagccga agatcgtcac
      961 gaccacgatg aagcaggtaa agaccacgat cccgacggcg tcatcgacgt cgagcagtcg
     1021 cccgatcgcc tgcccggcca gcacacagcc gctggcggaa aagccgatgt acatgaggca
     1081 tacggcgacg atcggtatgg ccgcgccgta gacgccgaac tgcacgcggc tggagatcat
     1141 ctgcgggagc ccgagctgcg gcccctgcgc cgcgtggagc gccatcaccg caccgccgat
     1201 cagctgcccg aggagcagcc cgatcagcga ccagaacaca tcgcccccaa acaccacggc
     1261 aagggcaccg gtgacgatgg cggtgatctg caggttcgcc gccagccaca gcgtgaattg
     1321 gctcgcgagc ccgccatgcc gctcggcgtc cggaatgtag tcgatcgagc ggacctcgat
     1381 caccgcaggc agcccggccg gcgcgctggc aactgacatc gccggattct tccacaaccc
     1441 ctcccgggtc gccacgcggg acgaattgct atcgcagctt ggtttcgtct agtctcagcg
     1501 ctgcccaaag ggggccgggg agtcaaacaa tgctcaagac gagaatcgcg atccttacgg
     1561 cagcggctgc ctgtctgctg gcggcctgca gcgacaacac gccagcgccg gcggcggcgc
     1621 ccgctccagc tgccagtgcg cccgctgcgc cggcgccagc accggccgcc gaccagtcgg
     1681 ccgcgcctgc agcagcaccg gcagcgggaa cggctgcggc gcctgcggcc gataccaaag
     1741 aagcgccgaa gcagtagagc ctgctcttgc gcgtgcacac ccccgggctt cggcccggcg
     1801 ggcgtgcttg cgctgcccga gtccgtcggc taaagcgccg gcagggggtt attttgccag
     1861 ctccatcagc agcatggtga gcaggtaaag ccggggagtg acgctgtcca actcgaggaa
     1921 ctccttgtcg gagtgaaacc cgccccccac cggcccgagt ccatcgagcg ccgccaccct
     1981 agcatccgcg gcgagcgctg actcggacgc gccaccgttg ccccccggga tgagcgtgcg
     2041 gccgatgccg gcgtaaatcc gctgcgcccg cgcagcgagt gcgtcagtgg cgggattgct
     2101 gggcaacggc gggaagccct ccccatgggt aacggtgaca cgggtgtcgg ggatcacggt
     2161 ggtctcggca ttcttgcgca aaactcccgc gacccgctcg aagtcggcct tgtcgcgtac
     2221 acgcacattc gccagcgccc aggcatcctc gggaatgatg ttgccgcgcg tgccggcgtg
     2281 catgacggtt agattcaccg tgagaccgtc gccggacttc ggaaagacct cgagccccga
     2341 gagctgatgg atcagctcga gtgccgcatt gcggccgtcc tggggagcga tacccgcgtg
     2401 tgcggcgcgc cctttgactt cgatgcggaa agtgtcggag cccttgcgcc agacggtgag
     2461 ggtatccggg aagtcccccg gctcgaggtt cagctcgacg tcggcgtcct tgaggagcct
     2521 gcctatcagg gctcgcgttc cgggcgagcc gcgctcctcc gaggtctcca tcagaaagac
     2581 gatcttgccg aaattcgtga agcccagatc gtggaggatc tgcagagcgt agaggccctc
     2641 gaccaccccg cctttctcat cggaaacgcc cgggccgtag gcccgcttgt catcggtgcg
     2701 aaaggggcgc tgttgcgcgg tgccgggctc aaacaccgta tcgatatgcc cgatcatcag
     2761 gatgcggcgc ttgccgcgac cggcgagcgt cgcgaccgtg ttctcggcaa gtccggcctc
     2821 ctccgccgga acggtctcga gcgtcatccc cagcgccttc aggcggggga tcagcacggc
     2881 cgccaccttg cggccgccct cgacgtcgcc cgtcccggaa tcgatgttga cgacctgctc
     2941 gagcagttgg agctcggcgg tgcgggaggc atgcgcggca ctgagtaccc tggcgttctt
     3001 ctccgttgcc gcacgagtga ccgccggtgc ggtcatggcg gcggcgatcg cgatgatgct
     3061 gatgagatgg ccgaacatgt tgcgaccccc cgctgctccg gttgtgccag gtgaataccg
     3121 aaccggttct ccaattcggc acggtcggcg tatgc
//
LOCUS       DATLKL010000104         3545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_1166, whole genome shotgun sequence.
ACCESSION   DATLKL010000104 DATLKL010000000
VERSION     DATLKL010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..948
                     /locus_tag="VM713_01350"
                     /pseudo
     CDS             19..948
                     /locus_tag="VM713_01350"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
     gene            945..2477
                     /locus_tag="VM713_01355"
     CDS             945..2477
                     /locus_tag="VM713_01355"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HVH82938.1"
                     /translation="MTSRRRESHRAGAAAGQAPRSIRYRHLVNPFEPVRLFSEDQVES
                     LHLAALGILEKQGMRVLSPRGRTVLASAGAAVDEATQMVRLDPAMVTAALTTVPREVN
                     LTARNPERTCRVGGRNVIFAPVAGPPSVSDLERGKRSGTLADYRDLVRLAQSFDVIHV
                     LGQMTEPGDAPVHERHLDTTLAQLTLGDKPPNFYSRGAGQLGDCFAMLRIAHGIDEET
                     FRREPRCYSICNTNSPLQLDLPMTDGIIEFALNGQLMIVTPFTLAGAMAPITIPGALT
                     LAHAEALFGITLTQIVRAGAPVMYGSFTSNVDMKSGSPAFGTPEYAKAAFGAGQLARR
                     IGVPWRSSSATASNAPDAQAAYEAQMSLWGALLGGCHFVLHAAGWLEGGLTASYEKFI
                     LDVEMLQMFAELFQPVPASSDDIGIEAVAEVGPGGHFFSTAHTMQRYRDAFYSPLVSD
                     WRNYGQWFDSGAKSATERATTVWRNTLSAFQPPAMDASVTEALEAFVTRRRAEGGAPP
                     QT"
     gene            complement(2481..>3545)
                     /locus_tag="VM713_01360"
     CDS             complement(2481..>3545)
                     /locus_tag="VM713_01360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HVH82939.1"
                     /translation="GVLAMRGLRIATLLVLVSLIAGTEFAAADSRAAAVPVPATMRAA
                     AIDRKGGPEVLTLHELPVPKPGPDEVLIALDTSGVGPWDIDVRERLDYWKNHNFPVVL
                     GVDGAGTVAALGANVRDFKVGDAVYSYAWDNPQGGFYAEYVAVPAKAIGHVPAGMSLK
                     DAGAMAVSALTALQGIDDALHIKSGETLIIHGASGAVGSLALQFARLRGAKVLATASG
                     DDGMSFVKRLGAGAAVDGRHGDIRAAARELAPGGVDAVLAFAGGDALETCIDALKPGG
                     RLAFPYGVRPEPKARAGLAAVRYDAVAGPKEYERLNEAIQAAKLEVPIAAEFPLAEAA
                     KAQERMAAGHVLGKIVLRVR"
BASE COUNT          564 a         1270 c         1182 g          529 t
ORIGIN      
        1 gactagtcgc ccgagcaggt ccatgcgctc ggtctctccg aggtggcgcg cgtcacgcgg
       61 gagatggatg ggttgctcag cgcgcatggg atcaccgccg gcagcgtcgg cgagcgcatg
      121 acggcgctcg ccgaggatcc gcgcttcctg ctgccgaacg atgccgaggg gcggcgtcag
      181 gcgctcgagc gctatcagaa gcttctcgac caggtggcgg cgcgcatgcc cgaatacttt
      241 cgtaccctgc cggccaggcg gctcagcgtc cagcgcgtgc cgccctcgca agagaagggc
      301 acctcgggag cgtattacca gcaagcggcg atggacggct cccggccagg tattttcttc
      361 gcgaatctcc gcgacatgag cgaggtgccg acctggcaga tgaacacgct cgcctaccac
      421 gagggaattc ccggccatca cctgcagatc tcgacggccc tcggactcaa ggacctgccg
      481 ctgatccgcc agcagacgct ctacaccgcg tacgccgagg gctgggcgct gtacgccgag
      541 cagctggccg ccgagatcgg catgtacacg gatgacccgt ggggcaatct cgggcgactg
      601 caggcggagc tgctgcgcgc cgcgcggctc gtcgtcgaca ccggcattca tgcccagggc
      661 tggacgcgcg agcaggcgat ctcctacatg gtcgccacca ccggcatggg cgaggcggag
      721 gtcacctccg aggtcgagcg gtacatggcc atgccgggtc aggcgtgcgc ctacaaggtc
      781 ggccagctta agatcctcga gctgcgcgcc aaggcgcgcg ccgcgctcgg accgaagttc
      841 aacctgaagg actttcacac cgtgattctg gaaaacggcg gcgtgccgct cacgcttctc
      901 gagcagctgg tcgacgagtg gatcgcgcgc acgcggggga caccatgacc tcccgccggc
      961 gcgagtcgca tcgggcgggg gcagctgccg gccaggcccc gcgctccatc cgctatcgcc
     1021 acctcgtcaa tcccttcgag ccggtccggc tcttctccga ggaccaggtc gagagcctgc
     1081 atctcgccgc gctcggcatc ctcgagaagc agggtatgcg cgtgctctcg ccgcgtggcc
     1141 gcacggtact cgcctcggcc ggagcggcgg tggacgaggc gacgcagatg gtgcggctcg
     1201 atcccgccat ggtgacggcg gcgctcacga cggtgccgcg cgaggtgaac ctcaccgcgc
     1261 gcaaccccga gcgcacctgc cgggtaggcg gccgcaacgt catcttcgcg cctgtcgcgg
     1321 ggcccccgag cgtgagcgat ctcgagcgcg gcaagcgctc cggcacgctc gccgactacc
     1381 gcgacctggt gcggctcgca cagtccttcg acgtgattca cgtgctcgga cagatgaccg
     1441 agcccggcga cgccccggtg cacgagcgtc acctcgacac gacactcgcg cagctcacgc
     1501 tcggcgacaa gccgccgaac ttctacagcc gcggcgccgg acagctcggg gactgcttcg
     1561 cgatgctgcg catcgcgcat ggaatcgacg aggagacctt ccggcgcgag ccgcgctgct
     1621 acagcatctg caacaccaac tcgccgctgc agctcgacct gcccatgacc gacgggatca
     1681 tcgaattcgc cctcaacggg caattgatga tcgtcacgcc gttcacgctt gccggcgcca
     1741 tggcaccgat caccattccc ggcgcgctca cgctcgcgca cgccgaggcg ctcttcggca
     1801 tcacgctcac gcagatcgtg cgggccggtg ctcccgtcat gtacgggagt ttcacctcga
     1861 acgtcgacat gaaatccggc tctccggcct tcggtacgcc ggagtacgcc aaggcggcct
     1921 tcggtgccgg gcagctcgcg cggcgcatcg gcgtgccatg gcgctcctcc agcgccaccg
     1981 ccagcaatgc gccggacgcg caggcggcct acgaggcgca gatgagcctg tggggagcgc
     2041 tgctcggcgg ctgccacttc gtgctgcacg ccgccggctg gctcgagggc ggcctcaccg
     2101 cctcgtacga gaaattcatc ctcgacgtcg agatgctgca gatgttcgcc gagctcttcc
     2161 agccggtacc cgcctcgagc gatgacatcg ggatcgaggc ggtcgcggag gtgggtcccg
     2221 gcgggcactt cttctccacc gcccacacca tgcagcgcta ccgcgacgcc ttctacagcc
     2281 cgctggtctc ggattggcgc aattacggcc agtggttcga cagcggcgcg aagagcgcca
     2341 ccgagcgggc gaccaccgtg tggcgcaaca ccctgagcgc ctttcagccg ccggcgatgg
     2401 acgcctcggt caccgaggcc ctcgaggcat tcgtgacgcg ccgccgcgca gagggcggtg
     2461 cgccgccgca aacgtaaagg tcagcgcacc cgcagcacga tcttaccgag cacatgcccg
     2521 gctgccatgc gctcctgcgc cttggcggct tcggcgagcg ggaattcggc agcgatcggc
     2581 acttcgagct tcgcagcttg gatagcctcg ttgagccgct cgtactcctt cgggcccgcg
     2641 accgcgtcat acctgacggc cgccagcccc gcgcgagcct tcggctcggg acgcacgccg
     2701 tagggaaagg ccagccgtcc gcccggcttg agagcgtcga tgcaggtctc gagcgcatcg
     2761 cccccggcga acgcgagcac cgcatcgacg ccgccgggcg cgagctcgcg cgcggccgcg
     2821 cggatgtcgc cgtgccggcc gtccaccgcg gcgcccgcgc cgaggcgctt cacgaaactc
     2881 atgccatcat cgccggatgc cgtcgcgagc accttcgcac cgcgcagcct ggcaaattgc
     2941 aacgcgaggc ttccgacggc gcccgaggcg ccgtgaatga tgagggtctc gccggacttg
     3001 atgtgcagcg cgtcgtcgat tccctgtagc gctgtgagcg cgctcaccgc catcgcaccc
     3061 gcgtccttca ggctcatgcc cgccggcacg tgtccgatcg ccttggccgg cacggcgaca
     3121 tactcggcgt agaagccgcc ttgggggtta tcccaggcgt aggagtacac ggcatcaccg
     3181 accttgaagt cgcgaacgtt cgccccgagc gccgcgacgg tgcccgcccc gtcgacgccc
     3241 agcacgaccg gaaagttgtg gttcttccag tagtcgagac gctcgcgcac gtcgatgtcc
     3301 cagggaccaa cgccggaggt gtcgagggcg atcagcacct catcggggcc tggcttggga
     3361 accggcagct catggagggt cagcacctcc gggccgccct tgcgatcgat ggcggcggcc
     3421 cgcatggtgg ccgggaccgg gacggctgca gcacggctgt cggccgcggc aaactcggtg
     3481 ccggcgatga gggacacgag gacgagcagt gttgcgatcc ttaacccgcg catggcgagg
     3541 actcc
//
LOCUS       DATLKL010000105         2286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_203910, whole genome shotgun sequence.
ACCESSION   DATLKL010000105 DATLKL010000000
VERSION     DATLKL010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203910"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2037
                     /locus_tag="VM713_01365"
     CDS             <1..2037
                     /locus_tag="VM713_01365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HVH82940.1"
                     /translation="GELMMLTLRAALLRLVGGALLASTAPAEALADSPGVSRALEALN
                     SVHAINEVALSPDGRHLVYGRLVRGRRGGADVEVSALYIVNAQDGSGETRLTACPGTV
                     CDEHGAAWSGDGKSIAFVATDEKEQPQIAVAAASGGDVRIITHAHGPLDTPRFSPDGK
                     RLAFLYSEGAPKTPGPLNPLARDAGVLSSTVYEQRLAVIPVQGGEPRLLGPGDLNIYE
                     YDWSPDGRRFVVSAAHGSGDDNWWIAELDLCDAESGAVTTLAKPSQQIASPRWSGDGK
                     RIAWIGGIMSDETITGGDVFVIPAAGGAAVDVTPGLAASVHTITWNDSATHLIATEFA
                     AGEEVLASIDAGAKSQRELWRAPEMIWAGNVFELTPGDVGISLSKNGAMSAVIRQSYT
                     TPPEVETGPPGRWRPITRINAGAHPITGKTTNVYWKSDTFTVQGMLVEPPSVTPGKSY
                     PLVVLVHGGPAHAHYPVFPANPHSFDAVLASEGYYVFMPNPRGSYGQGEAFTEANVKD
                     FGYGDLRDIMAGVDTVLKSAPVDPARLGIAGWSYGGYMTMWALTQTNRFKAAVAGAGL
                     SDWLSYYGTNNIDTWMIPYFGTTVYDDPKVYERSSPITFIKNVHTPTLMVGGDRDAEV
                     PISQSYEYWNALQRLGVKTEFVVYPDEGHYFFKHDDQVDVLTRLVGWFDQYLKP"
     gene            complement(2060..>2286)
                     /locus_tag="VM713_01370"
     CDS             complement(2060..>2286)
                     /locus_tag="VM713_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82941.1"
                     /translation="KFERELAAKNHGDAWVPYELAGALYAQALAEPARRQALLHEAAV
                     LIDGLASELKPLHDVRRLRGSVEKEAQRGS"
BASE COUNT          364 a          803 c          757 g          362 t
ORIGIN      
        1 ggtgagctca tgatgctgac gctgcgcgcg gctctgcttc gactcgtcgg cggcgcgctg
       61 ctcgcctcga cggcgccggc ggaggcactc gcagactctc cgggcgtcag ccgtgcgctc
      121 gaggcgctca actccgtgca cgccatcaac gaggtggcgc tctcgccgga cggcaggcat
      181 ctcgtctatg gccgcctcgt gcgcggcagg cgcggcggcg ccgacgtgga ggtctcggcg
      241 ctctacatcg tcaacgcaca ggacggcagc ggcgagacgc ggctgaccgc ctgtcccggc
      301 acggtgtgtg acgagcacgg ggccgcctgg tcgggcgatg gaaagtcgat cgctttcgtc
      361 gccacggacg agaaggagca gccgcagatc gcggtcgccg cggcctccgg cggcgacgtg
      421 aggatcatca cccacgcgca cggcccgctc gacacgccgc gcttctcgcc ggacggcaag
      481 cgcctcgcat tcctctactc cgagggcgcg ccgaagactc ccgggcccct gaatccgctc
      541 gcgcgcgatg cgggggtgct ctcctcgacg gtttacgagc agcgcctggc agtcattccg
      601 gtgcagggcg gcgagccgcg gctcctcggc cccggcgatc tcaatatcta cgagtacgac
      661 tggtcgcccg acggccgccg cttcgtcgtg agcgccgccc acggctcggg agatgacaac
      721 tggtggatcg ccgagctcga tctgtgcgat gccgagagtg gcgcggtgac gacgctcgcg
      781 aagccgtccc agcagatcgc ctcgccccgc tggtctggcg acggcaagcg catcgcctgg
      841 atcggcggca tcatgagcga tgagaccatc accggcggtg atgtgttcgt gattccggcc
      901 gcgggaggcg ctgcggtcga tgtcacgccg ggtctcgcgg cctctgtgca caccatcacc
      961 tggaacgact cggccacaca cctcatcgcc accgagttcg cggcgggcga ggaagtgctc
     1021 gcctcgatcg acgccggcgc caaatcgcaa cgcgagctgt ggcgtgcgcc cgagatgatc
     1081 tgggccggta acgtctttga gttgaccccg ggcgacgtcg gcatctcgct ctcgaagaat
     1141 ggcgcgatgt cggccgtcat ccgccagtcc tacacgacgc cccccgaggt cgagaccggg
     1201 ccgccgggca ggtggcggcc gatcacccgg atcaacgccg gcgcgcaccc gatcaccggc
     1261 aagacaacga acgtctactg gaagagcgac accttcacgg tccagggcat gctggtcgag
     1321 cccccgtccg tcacccccgg caagagctat ccgctggtgg tgctggtgca cggcgggccg
     1381 gcgcatgccc actatccggt cttcccggcg aacccacact ccttcgacgc cgtgctcgcc
     1441 agcgagggct actacgtctt catgccgaac ccccgcggca gctacggtca gggcgaggcc
     1501 ttcacggagg ccaacgtcaa ggacttcggc tacggcgatc tgcgcgacat catggcgggg
     1561 gtcgacacgg tgctgaagag cgcccccgtc gatcccgccc gtctcgggat cgctggctgg
     1621 agctacggcg gctacatgac catgtgggct ctgacccaga ccaatcgctt caaggcggcc
     1681 gtcgcgggtg cgggactctc cgactggctg agctactacg gcaccaacaa catcgatacc
     1741 tggatgatcc cctacttcgg gaccacggtg tacgacgatc ccaaggtcta cgagcgcagc
     1801 tcgccgatca ccttcatcaa gaacgtgcac acacctaccc tcatggtggg cggcgaccgc
     1861 gacgcggaag tgccgatcag ccagtcctac gagtactgga acgcgctgca gcgcttaggg
     1921 gtcaagactg agttcgtcgt ctacccggac gaggggcact acttcttcaa gcatgacgac
     1981 caggtcgacg tcctgacgcg gctcgtcggc tggtttgatc agtacctgaa gccctagcgg
     2041 ttagcgcagg gcggtgcggt cagctcccgc gctgcgcctc cttctcgaca cttccccgca
     2101 accggcgcac gtcgtgcagg ggcttgagct cggacgcgag tccgtcgatg agcaccgcgg
     2161 cctcgtgcag cagcgcctgg cgccgcgcgg gctcggcgag cgcctgcgcg tacagtgcgc
     2221 ccgcgagttc gtaaggcacc cacgcgtcgc cgtggttctt cgccgcgagc tcgcgctcga
     2281 acttca
//
LOCUS       DATLKL010000106         2399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_181625, whole genome shotgun sequence.
ACCESSION   DATLKL010000106 DATLKL010000000
VERSION     DATLKL010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181625"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1122
                     /locus_tag="VM713_01375"
     CDS             55..1122
                     /locus_tag="VM713_01375"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF003776.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HVH82942.1"
                     /translation="MHRNFDGRRHKSTLETKGAMPVKIDPYDAARRSVYSTAAGSADN
                     GAITLGLVNNMPDAALESTEAQFSGLLSAAAGSRPLHLRFCSFPELPRGIDAREHIGR
                     SYWPIETLLAERPDALIVTGTEPIAARLPDDPYWSRLTALMEFAEQHTAASIWSCLAA
                     HAAAEYFDGIKRRRLPEKRCGVYVHSLLPRHPLLAGVAAPLAMPHSRWNELPVDELRV
                     AGYQVLSFAPETGADAFVRHERSLMLFFQGHPEYEDTTLLKEYRRDVGRYLSGQQAHY
                     PTRPHGYLSPEAIALLERFKERAIAERTPSLLEQFPFAAVAAGLQNTWRPAAVTLYRN
                     WLDFIATGRTRGAEEVSLAGL"
     gene            1119..1403
                     /locus_tag="VM713_01380"
     CDS             1119..1403
                     /locus_tag="VM713_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HVH82943.1"
                     /translation="MSPATEDQNPQSAARARLLGLVEKVLERPGAGPTLPPDAALSEL
                     GVSSLKMVSLMLSVEAEFDLSIPQNEITPENFRSINSIDALVKRLMAFKG"
     gene            complement(1447..>2399)
                     /locus_tag="VM713_01385"
     CDS             complement(1447..>2399)
                     /locus_tag="VM713_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH82944.1"
                     /translation="HTLFAHILPRAGIKAHLAKSDRPAELEALIDANTRALFCESVGN
                     PAGNICDLEALAAIAQRARIPLVVDNTVATPVLLRPFEYGADIVVHSLTKFLGGHGTT
                     LGGAIVDSGRFRWKEHAARFPRFTQPDESYHGLVYTDHYGDKAFLGRARSVFQRAMGA
                     VLSPFNAFLLLQGIETVSLRVERHVENARRVAEFLRGDRRVASVSYVGFPDNPYHALC
                     QKYLGGHACSLLTFAICGGFDAGTRFYDALKLVKRLVNLGDAKSLACHPASTTHRQMS
                     AEEQRQAGIGPETIRLSVGIEHVDDILEDLDQALAAATSG"
BASE COUNT          434 a          785 c          772 g          408 t
ORIGIN      
        1 tagactcccg attgttacgc tttgtgttgg gaacatagcg caagcggccc cataatgcac
       61 cgcaacttcg atggccggcg tcacaaatct acgctggaaa ctaagggtgc catgccggta
      121 aagatcgacc cctacgacgc cgcgcggcgt tcggtgtact ccacggcggc ggggagcgca
      181 gataacggcg cgatcaccct cggcctagtg aacaatatgc cggacgctgc gctcgagtcg
      241 accgaggcgc agttctcagg cctcctctcg gcggcggccg gttcgcggcc gctgcatctg
      301 cgcttctgct ccttccccga gctgccgcgc ggcatcgacg cacgtgagca catcggccgc
      361 tcttactggc cgattgagac gctccttgcc gagagacccg atgctctcat cgtcacgggg
      421 accgagccga tcgccgcgcg actccctgac gacccctact ggtcacgcct cactgcactc
      481 atggagttcg ccgaacagca cacagcggcg agcatctggt cgtgcctcgc tgcgcacgcg
      541 gcggcagaat acttcgacgg catcaagcgc cggcgactcc cggagaagcg ctgcggcgtc
      601 tatgtccaca gcttgctccc gcgccatccg ctgctcgctg gcgtcgccgc gccgctcgcc
      661 atgccgcact cgcggtggaa cgagctaccg gtcgatgagc tgcgggtagc cggttaccag
      721 gtcctgagct tcgctcccga gaccggagca gatgcctttg tgcgtcacga acgcagtctc
      781 atgctcttct tccagggcca cccggaatat gaggacacga cgttgctcaa ggaataccgg
      841 cgcgacgtgg gccgatattt atccggccag caggcccatt acccgacgag gcctcatggg
      901 tacttgtcgc cagaagcgat tgcgctgctc gaacgattca aagagcgcgc gatcgccgag
      961 cggacgccgt cgctcctcga acagtttccc ttcgccgccg tggccgcagg gcttcagaac
     1021 acctggcgcc cggcggctgt caccctctat cgcaactggc ttgacttcat cgccacgggg
     1081 aggacgcggg gcgcggaaga agtatcatta gccggcctat gagcccggcc accgaggatc
     1141 aaaatccaca atccgcagcg cgtgcccgcc tgctcggtct ggtcgagaag gttctcgaac
     1201 gacccggcgc gggaccgacg ttgccgcccg acgccgcgct gagtgagctc ggcgtgagct
     1261 ctttgaagat ggtgagcctc atgctctcgg tcgaagccga gttcgacttg agcatccctc
     1321 agaacgagat cacgccggag aatttccgat caatcaactc catcgacgca ctggtgaagc
     1381 gcctcatggc gttcaagggc tagcgattac gataacacca gcacgacctt tctttcagcg
     1441 ggcccttcag cctgaggtcg ccgccgcgag cgcttgatca agatcctcga ggatgtcatc
     1501 cacatgctcg atgccgacgc tcagacggat cgtctcaggg ccgatgcccg cctggcgctg
     1561 ctcctcggca gacatctgcc ggtgcgtggt cgaggccgga tggcaggcga gcgacttggc
     1621 gtccccgagg ttcaccaggc gcttgacgag cttgagcgcg tcgtagaagc gcgtgccggc
     1681 atcgaaaccg ccgcagatcg cgaaggtcag aagtgagcac gcgtgcccgc cgagatattt
     1741 ctggcacagc gcgtggtagg gattatccgg aaagccgacg tagctcaccg atgcgacgcg
     1801 gcggtcgcca cgcaggaact ccgccacccg gcgcgcgttc tcgacgtgcc tctcgacgcg
     1861 gagcgacacc gtctcgatgc cctggagcag gaggaacgcg ttgaacggcg acagcaccgc
     1921 gcccatggcg cgctgaaaga cgctgcgggc acggccgaga aaggccttgt cgccgtagtg
     1981 atcggtgtaa acgagcccgt gataggactc gtcgggctgg gtgaagcgcg ggaaacgcgc
     2041 ggcgtgttct ttccagcgga agcggccgct gtcgacgatc gcgccgccga gggtcgtgcc
     2101 gtggccaccg aggaacttgg tgagcgaatg caccacgatg tcggcgccat actcgaaggg
     2161 cctgagcagc accggggtcg cgaccgtgtt gtcgacgacc agcgggatgc gcgcgcgctg
     2221 cgcgatggcc gcaagcgcct caagatcgca gatgttgccg gcggggttgc ctacggactc
     2281 gcagaacagc gcacgcgtgt tcgcatcgat cagtgcctcg agctcggcgg gccgatcgga
     2341 tttcgcgagg tgcgccttga tgccggcacg cggcaggatg tgcgcgaaca gagtgtgcg
//
LOCUS       DATLKL010000107         5333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_136533, whole genome shotgun sequence.
ACCESSION   DATLKL010000107 DATLKL010000000
VERSION     DATLKL010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136533"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VM713_01390"
     CDS             complement(<1..632)
                     /locus_tag="VM713_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82945.1"
                     /translation="MLLLVLNAGSSSLKFDLLDWSGTGAARRLKAGAFIDKGDGSGSF
                     ALESALPVPPPPAPVRTLAAAADFVLAWLADQNAHGRNLLAEVRATVHRIVHGGELFR
                     ATARLGAADLAALATLDALAPLHNPPALAVIAAARARLGERLPLIGVFDTAYYAQLPE
                     AAWRYAVPAHWYQDLGVRRYGFHGTAHRYLCATARARVLPGKPARRIVSL"
     gene            complement(632..2017)
                     /locus_tag="VM713_01395"
     CDS             complement(632..2017)
                     /locus_tag="VM713_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVH82946.1"
                     /translation="MSPSAALEGLAASGLRRSLQRRHMQMIALGGVIGAGLFVGSGVV
                     IGSAGPAAVISFALTGTLVVLVMRMLGEMAVAYPAVGGFYEYNRLALGKLAGFLTGWM
                     YWYFWVIVVALEAVAGAKILGTWWPQIAPWEFTLALMALFTVVNLLSVRSYGEAEFWF
                     ASIKVAAIVVFLVAGALFVFGGWPGAHGGLPHLTADGGFMPNGPVPVLTGAVAATGFY
                     FGAEIVTIAAAESAEPERAVAETTQSVIWRVLVFYVGSIFLVVALVPWNDTVRMSRPY
                     VSVMEVLRIPAAGTVMSLVILTAVLSALNSGLFASSRMLMALAGHGDAPAAVARLDRR
                     GVPVAAILASTAFGYVTVAMSYLSPERIFAFLVNSYGTVAIFVYVLISVAELRLRRRL
                     EREAPERLRVRMWGYPWLTRVAIVGMLAILAAMAVIPGQRVSLGFGLVSAAILLGAWL
                     VRAALKRTVRP"
     gene            complement(2055..3809)
                     /gene="xsc"
                     /locus_tag="VM713_01400"
     CDS             complement(2055..3809)
                     /gene="xsc"
                     /locus_tag="VM713_01400"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HVH82947.1"
                     /translation="MTQPQKMGPSEALVETLAANGVTHVFGIVGSAFMDALDIFPAAG
                     IRFVPVAHEQGAIHMADGYARASGRHGVCIAQNGPGVTNFVTGVAAAYWAHSPVVFIT
                     PETGSMTMGLGGFQETEQLPIFSKITKYQGHVNNPRRMAEIAARCFDRAMLEMGPTQL
                     NIPRDYFYGEIETAIPSPMRIAHGAGDAAALDAAADLLAGATFPVIVAGGGVITAGAT
                     TEATQLADALGAAVCSSYLHNDAFPASHPLAAGPLGYQGSKAAMKLIARADVVLALGT
                     RLGPFGTLPQYGLDYWPKLAKIIQIDSDPRMLGLVRPISVGIHGDARAAAAALLVRLK
                     TRKLAGAATRAERLARVAAEKSDWEAELGSWTHERDPYSLEVAAGSKYLHPRQVLRAL
                     ERALPAGAMVSTDIGNICSVANSYLRFDRPRSMFAAMSFGNCGYAFPVIRGAKIAAPE
                     RPAFAYVGDGAWGISFNELLTCAREKIGVTPIVFNNGQWGAEKKNHVDFYSRRYQGVN
                     LENPSWAKVAQAFGCEGVTVERLADIEPVLRAAAAAQSDGRTTVVEMMVTQELGDPFR
                     RDALSKPVRHLAKYQKFV"
     gene            3953..4744
                     /locus_tag="VM713_01405"
     CDS             3953..4744
                     /locus_tag="VM713_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVH82948.1"
                     /translation="MNRVLSIPAEVADIAAETSSTLKAFGVLEVLVRAGRAVTLSELM
                     QATDLPKPTLHRTLALFEEAGFLAREPGGRAYIIGERLSRFALDVLRSDGAAAQRRTI
                     LRRVVSEITETCNLAVLKKAALVYLDRVEAPWPLRLYLPEGGGLPPHCCASGKLLLAH
                     LPAAERQGLLELLPLERFTERTITDRAVLDSELDRIVSVGYAVDNEEYVLGVACVAVP
                     VRNRHGDVVASLAVQGATARLPLMRAIEFIPRLRAAAAEIGSTFG"
     gene            4764..>5333
                     /locus_tag="VM713_01410"
     CDS             4764..>5333
                     /locus_tag="VM713_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH82949.1"
                     /translation="MKVENLERHWLPFTNNREFKREPRLFVRASGMHYYTPSGRKVLD
                     GSSGLFTSAAGHCRPEIAAAVGKQLETLDFTPSFQRSHPGAFELADGVAGLTPQGLDH
                     VFFCNSGSEAVDTAMKVALAYHLARKEGQRNYFVSRERAYHGVNIGGTALSGMVRNRE
                     VFGALMPGILHLRHTLLPENRLTRGLGEKG"
BASE COUNT          862 a         1823 c         1888 g          760 t
ORIGIN      
        1 tgcaggctca cgatccggcg ggccggcttg cccgggagca cccgcgcgcg cgccgtggca
       61 cacaggtagc ggtgcgcggt gccgtgaaag ccgtagcggc gtacgccaag gtcctggtac
      121 cagtgggcgg gaacggcgta acgccacgcg gcttcgggca gctgcgcgta gtaggcggta
      181 tcaaatacgc cgatgagcgg cagccgctcg ccgagccgcg cccgcgccgc cgcgatcacg
      241 gcgagcgccg gcgggttgtg caagggcgcg agcgcatcca gcgtagcgag cgcggcgagg
      301 tctgcggcgc cgagccgcgc cgtcgcccgg aagagctcgc cgccgtgaac gatgcggtgc
      361 acggtcgccc gcacttccgc gaggagattc ctgccgtgcg cgttctggtc cgcgagccac
      421 gcgagtacga agtccgcggc cgcggccagc gtacggacgg gggccggggg cgggggcacg
      481 ggaagcgcgc tctccagggc gaagctcccc gagccgtcgc ccttgtcgat gaacgcgccg
      541 gccttgaggc ggcgcgccgc tcccgtcccc gaccagtcga ggagatcgaa tttgagcgag
      601 gagctgccgg cgttgaggac gaggagcagc atcacggccg caccgtgcgc ttgagggccg
      661 cgcgcaccag ccaggcgccg agcaggatgg cagcgctcac caggccgaag ccgagcgaca
      721 cgcgctgccc gggaatcacc gccatggcgg cgaggatcgc cagcatccca acgatcgcga
      781 cgcgcgtgag ccacgggtaa ccccacatgc ggacccgcag cctctcgggc gcctcgcgct
      841 cgaggcggcg ccgcaggcgc agctccgcca cactgatcag cacgtagacg aagatcgcga
      901 ccgtgccgta ggaattgacg agaaaggcga agatgcgctc gggcgagagg tagctcatcg
      961 cgaccgtcac gtagccgaag gcagtgctcg cgaggatggc ggcgaccggc actccgcgcc
     1021 tgtcgaggcg cgcgacggcc gctggtgcat cgccgtgccc ggcgagcgcc atcagcatgc
     1081 gcgaggaggc gaacaggccc gagttgagcg ccgagagcac cgcggtcagg atcacgaggc
     1141 tcatcacggt gccggcggcg ggaatgcgca gcacctccat gacgctcacg taggggcgcg
     1201 acatgcgcac cgtgtcgttc cacggcacga gggcgacgac caggaagatc gagccgacgt
     1261 agaagacgag cacgcgccag atcaccgact gcgtggtctc cgcgaccgct ctctcgggct
     1321 cggccgactc ggcggcagcg atggtgacga tctcggcgcc gaagtagaag ccggtcgcgg
     1381 ccaccgctcc ggtgagcacc ggcacggggc cattcggcat gaagccaccg tcggccgtca
     1441 ggtgcggcag cccgccgtgc gcgcccggcc agccgccaaa gacgaacagc gccccggcga
     1501 cgaggaacac gacgatcgcc gccaccttga tcgaggcgaa ccagaactcc gcctccccgt
     1561 aggagcgcac cgataacagg ttcacgacgg tgaacagcgc catgagggcg agcgtgaact
     1621 cccagggcgc gatctgcggc caccaggtgc cgaggatttt cgcccccgcg accgcctcga
     1681 gcgcgaccac gatcacccag aagtaccagt acatccagcc ggtgagaaat cccgcgagct
     1741 taccgagcgc caggcggttg tattcataga acccgccgac cgcggggtac gcgaccgcca
     1801 tctccccgag catgcgcatc accagcacga cgagcgtccc ggtgagggcg aaggagatga
     1861 ccgccgcggg gcctgccgag ccgatgacga ccccgctgcc gacaaagagc ccggcgccga
     1921 tcaccccgcc gagcgcgatc atctgcatgt gacgccgctg cagcgagcgg cgcagcccgc
     1981 tcgcggcgag gccctcgagc gctgcgctcg ggctcacggg cgccgcgtcc ccggggacgt
     2041 gcaggcgatc ggcttcacac gaacttctgg tacttggcga ggtggcgcac gggcttggag
     2101 agcgcgtcgc ggcggaacgg gtcgccgagc tcctgggtga ccatcatttc gaccacggtg
     2161 gtgcgcccgt cgctctgtgc ggcggccgcc gcgcgcagca ccggctcgat gtcggcgagc
     2221 cgctcgacgg tgacaccctc gcagccgaag gcctgcgcca ccttcgccca gctcggattc
     2281 tccaggttca cgccctggta gcggcgcgag tagaagtcga cgtgattctt cttctcggcg
     2341 ccccactggc cgttgttgaa cacgatcggg gtcacgccga tcttctcgcg cgcgcaggtg
     2401 agcagctcat tgaagctgat gccccaggcg ccgtcgccca cgtaggcgaa cgccgggcgc
     2461 tcgggggcgg cgatcttcgc tccgcggatg accgggaagg cgtagccgca gttgccgaag
     2521 ctcatggccg cgaacatcga gcgcggccga tcgaaccgca ggtaggagtt ggcgaccgag
     2581 cagatgttgc cgatgtcggt ggacaccatc gcgccggccg gcagcgcgcg ctcgagcgcg
     2641 cgcagcacct gccgcggatg caggtacttc gagccggcgg ccacctcgag cgaataggga
     2701 tcgcgctcgt gcgtccagct gccgagctcc gcttcccagt cggacttctc cgccgcgacg
     2761 cgtgccaggc gctcggcgcg cgtggcggct ccggcgagct tgcgggtctt gagacgcacg
     2821 aggagggcgg cggccgcggc ccgggcatcg ccgtggatgc cgacggagat cggcctgacg
     2881 agcccgagca tgcgtgggtc ggagtcgatc tggatgatct tggccaactt cggccagtag
     2941 tcgaggccgt attgcggcag ggtgccgaag gggcctaagc gcgtgccgag cgcgagcacg
     3001 acgtccgcgc gcgcgatgag cttcatggcg gccttcgagc cctgatagcc gagcgggccc
     3061 gcggcgagcg gatgcgaggc ggggaaggca tcgttgtgca ggtagctgct gcagaccgcc
     3121 gcgccgagcg catcggcgag ctgcgtcgcc tcggtggtgg cgccggctgt gatcaccccg
     3181 ccaccggcga cgatcaccgg gaaagtggcg ccggcgagca gatctgcggc ggcatcgagc
     3241 gcggccgcat ctccggcacc gtgcgcgatg cgcatggggg aggggatcgc ggtctcgatc
     3301 tcgccgtaga aataatcgcg cggaatgttg agctgcgtcg gccccatctc gagcatggcg
     3361 cgatcgaagc agcgcgcggc gatctccgcc atgcgacgcg ggttgttcac gtgtccctgg
     3421 tacttggtga tcttcgagaa gatcggcagc tgctcggtct cctggaagcc gccgaggccc
     3481 atcgtcatcg agccggtctc aggggtgatg aacacgaccg gcgaatgcgc ccagtaggcc
     3541 gctgccactc cggtgacgaa gttggtgacg ccgggaccgt tctgcgcaat acagaccccg
     3601 tggcggcccg aggcgcgcgc gtagccgtcg gccatgtgga tcgcaccctg ctcgtgagcg
     3661 acgggcacga agcggatgcc cgcggcggga aagatatcga gggcgtccat gaaggccgag
     3721 ccgacgattc cgaacacatg ggtgacgccg ttggcggcga gcgtctcgac gagcgcctcg
     3781 gaggggccca tcttctgcgg ctgggtcatc gttggctccg gcgtgtttct tcagatgaaa
     3841 tgatttgtct ctttgagctc gcggagtcta caatacctgc ctcgtagagc aaacagtgtt
     3901 cctgtatcta ggcgcttgag tctcgtttaa cggaacctag gaccccgttg gaatgaaccg
     3961 tgtcctctca atccccgcgg aggtcgcgga catcgccgcc gagacctctt cgacgctcaa
     4021 ggccttcggc gttctcgagg tactggtacg cgccggtcgt gccgtgacgc tctccgagct
     4081 gatgcaggcc acagacctcc cgaagccaac gctgcaccgc acgctcgcgc tattcgagga
     4141 ggccgggttc ctcgcccgcg agccgggcgg ccgcgcctac atcatcggcg agcggctgtc
     4201 gcgcttcgcc ctcgacgtgc tgcgcagcga cggcgccgcc gcccagcggc gcacgatcct
     4261 gcgccgcgtg gtgagcgaga tcaccgagac ctgcaatctc gcggtgctga agaaggctgc
     4321 gctcgtctac ctcgatcgtg tcgaggcgcc ctggccgctg cggctgtacc tgcccgaagg
     4381 cggcgggctg ccgccgcact gctgtgcgag cggcaagctg ctgctcgcgc acctgccggc
     4441 ggctgagcgg cagggcctgc tcgagctgct gcctctcgag cgcttcaccg agcgcacgat
     4501 caccgaccgc gcggtgctcg attccgagct cgaccggatc gtgtccgtcg gctacgccgt
     4561 cgacaacgag gagtacgtgc tcggcgttgc ctgcgtggcg gtgccggtgc gcaaccgcca
     4621 cggcgatgtc gtagcctcgc tcgcggtgca gggggcgacc gcacggctgc ccctgatgcg
     4681 cgccatcgaa ttcatcccgc gcctgcgcgc ggcggcggcg gagatcggct ccaccttcgg
     4741 ctgatgcgga gatcgacatg gacatgaagg tcgaaaacct cgaacggcac tggctgccgt
     4801 tcacgaacaa ccgcgagttc aagcgcgagc cgcgcctgtt cgtgcgtgcg agcggcatgc
     4861 actactacac cccgagcggc cgcaaggtcc tcgacgggag ctcggggctg ttcaccagcg
     4921 ccgccggtca ctgccggccg gagatcgcgg ccgcggtcgg gaagcagctc gagacgctcg
     4981 acttcacgcc gtccttccag aggagccatc cgggcgcctt cgagctcgcc gacggcgtgg
     5041 cgggcctgac gccgcagggc ctcgaccacg tgttcttctg caactccggc tccgaggcgg
     5101 tcgacaccgc catgaaggtc gcgctcgcgt atcacctggc gcgcaaggag ggccagcgca
     5161 attatttcgt ctcgcgcgag cgtgcctatc acggtgtcaa catcggcggc acggcgctct
     5221 ccggcatggt gcgcaatcgc gaggtgttcg gcgccctgat gcccggaatc ctgcatctgc
     5281 ggcacacgct gctgcccgag aaccgcctga cgcgcggcct gggcgagaag ggc
//
LOCUS       DATLKL010000108         2102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_113987, whole genome shotgun sequence.
ACCESSION   DATLKL010000108 DATLKL010000000
VERSION     DATLKL010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113987"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VM713_01415"
     CDS             <1..1160
                     /locus_tag="VM713_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH82950.1"
                     /translation="LITMGARQSLGLFVAPITKATGLGIVAISLAMAIGQFVWGLTQP
                     IFGAVADRFGSGAVLVAGAVTLAAGATLTTAVSSELGLILAIGVLFAAGAAGGSFSVL
                     IGATARRLAPEKRAFASGVINAGGSLGQFLLAPISQLLISSFGWMVAMYALALAALAT
                     VPLAAPLLKSSPAPEQPGPHEGGGPLRAQLRAAARDRSYWCLHLGFFTCGFHIAFLVT
                     HLPGEVNLCALPASVAAGSLAIIGLSNVAGSLAIGWLGGLYRMKWLLFFVYFLRAVAI
                     GVYLMLPRTPLTFYGFAAVLGLTWLSTVPPTAGLVGKLFGVRYLATLFGLTLLSHQIG
                     GFFGAWLGGIALVRFGDYGWMWYADMTLALGAALINLPIREARLVPAPAAA"
     gene            1192..1863
                     /locus_tag="VM713_01420"
     CDS             1192..1863
                     /locus_tag="VM713_01420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVH82951.1"
                     /translation="MNQCSFLFDLDGTLVDSVYQHVAAWQQALHAADIELSVWRIHRR
                     IGMSGGLFAEMLRREIDREITPALLEKLRADHAAAYIGMRKWVRPLPGAVRLLEHLRR
                     AGIPFAIATSGRMITAKYNLEALGVDADTTVVVTRDDVRRGKPDPDLFVAAAERLGAD
                     VEASVIVGDSIWDMLAARRARGLGVGLLSGGYGEDELERSGAIRVYDDPADLLRHIDE
                     IGGRV"
     gene            complement(1910..>2102)
                     /locus_tag="VM713_01425"
     CDS             complement(1910..>2102)
                     /locus_tag="VM713_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HVH82952.1"
                     /translation="IGAKCLWCQFGVINEEGARIAERGGLTVVMDRCLKVEHARYAGR
                     MHWLGFNTQRITSVRSGLQ"
BASE COUNT          289 a          700 c          741 g          372 t
ORIGIN      
        1 tcctcatcac gatgggcgcg cgtcagtccc tcggactgtt cgtcgcgccg atcaccaaag
       61 cgaccggact cggcatcgtg gcgatcagcc tggcgatggc gatcggacag ttcgtctggg
      121 gactgacaca gccgatcttc ggtgcggttg cggatcgctt cggctccggc gcggtgctag
      181 tcgcgggggc ggtaacgctc gccgcagggg cgacactgac gaccgcggtc tcgagcgagc
      241 tcgggctgat actcgcgatc ggagttctgt tcgcggcggg tgcggcgggc ggcagcttct
      301 ccgttctgat cggtgccacg gcgcgccgtc tcgccccgga gaagcgcgcc tttgcgtccg
      361 gcgtcatcaa cgcgggaggg agcctcgggc agttcctgct ggcgccgatc agtcagctgc
      421 tcatctcgag cttcggctgg atggtcgcaa tgtacgcgct ggccctggcg gcacttgcga
      481 ccgtgccgct ggcggcgccg ctgctcaagt cctcgcctgc gcctgagcag cccggcccgc
      541 acgagggcgg cgggcccctg cgcgcgcaac tgcgggcggc ggcgcgcgac cgcagctact
      601 ggtgcctgca ccttgggttc ttcacctgcg gcttccacat cgccttcctg gtcacccacc
      661 tgccgggcga ggtgaacctg tgcgccctgc cggcgagcgt cgcggccggc tcgctcgcga
      721 tcatcggcct gtcgaatgtc gccgggagcc ttgccatcgg ctggctggga ggcctgtacc
      781 gcatgaagtg gctgctgttc ttcgtatact tcctccgcgc cgtcgcgatc ggcgtctacc
      841 tgatgctgcc gaggacgcca ctcactttct atggattcgc cgccgtgctc gggctgacgt
      901 ggctgtcgac ggtcccgccg acggcgggcc tggtgggcaa actcttcggc gtccgctacc
      961 tggcgacgct ctttggtctc acgctgctct cgcaccagat cggcggtttc ttcggcgcat
     1021 ggctcggcgg catcgcgctg gtacgcttcg gtgactacgg ttggatgtgg tacgccgaca
     1081 tgacactggc gctcggcgcc gcgctcatca acctgccgat acgcgaggca cggctggtgc
     1141 ctgcgccggc cgcagcgtga acggtccgac tgcaagaggc tcgagcaacc gatgaaccaa
     1201 tgcagctttc tcttcgatct cgatggcacg ctggtcgaca gcgtctacca gcacgtggcg
     1261 gcctggcagc aggcgctgca cgccgcggac atcgagcttt cggtgtggcg gattcaccgg
     1321 cgtatcggca tgagcggcgg gctgtttgcc gagatgctgc gccgggaaat cgatcgcgag
     1381 atcacccccg cgctgctgga aaagctgcgc gcggatcatg ctgcggcgta catcggcatg
     1441 aggaaatggg tgcggccgct cccgggggcg gtacgcctgc tcgagcacct gaggcgcgcc
     1501 ggcatcccct tcgccatcgc caccagcggc cggatgatca ccgcgaaata caacctcgag
     1561 gcgctgggcg tagatgctga caccacggtc gtcgtgacca gagacgatgt gcggcgtggc
     1621 aagccggacc cggacctgtt cgtggcggcg gcggagcggt tgggcgcgga cgtcgaggca
     1681 tccgtgatcg tcggtgacag catctgggac atgctcgcgg cgcggcgcgc ccggggactc
     1741 ggggtggggt tgctgtcggg aggatacggc gaggatgagc tcgagcgcag cggtgcgatt
     1801 cgcgtctacg acgatccagc ggacctgctg cgccacatcg atgagatcgg cgggcgcgtc
     1861 tgagggcctg aggcgagcgc cgtacctggc gccgcgcccg gccgccgcgt tactgcaggc
     1921 cgctccgcac cgaggtgatg cgctgcgtgt tgaagccgag ccagtgcatg cgtcccgcgt
     1981 aacgcgcgtg ctccaccttg aggcaacggt ccatgacgac ggtgaggccg ccacgctcgg
     2041 cgatgcgcgc gccttcctcg ttgatcaccc cgaactggca ccacaggcac ttggcgccga
     2101 tc
//
LOCUS       DATLKL010000109         2285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_159019, whole genome shotgun sequence.
ACCESSION   DATLKL010000109 DATLKL010000000
VERSION     DATLKL010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159019"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VM713_01430"
     CDS             <1..735
                     /locus_tag="VM713_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH82953.1"
                     /translation="VTWGAQAKWYGFPRTPFDVSFIARGATASIAEHVTTDTAAVIVE
                     PVQGLAGAVALGAPFLAALRARCDEVGALLIFDEVQCGMGRTGEPFAANLYGVRPDML
                     TTAKALGNGFPVAALMMSPLVSASLKTEALGTTFGGGPMACAAVIAVLAAIREQHLLE
                     RVRRIGAYIRTSCITGPVTAASGAGLLVGLRTTRPAKEIQGELLECGILTGTSSDPQV
                     LRLLPPYILEEEHVDLLRDALKDLEA"
     gene            732..1730
                     /locus_tag="VM713_01435"
     CDS             732..1730
                     /locus_tag="VM713_01435"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HVH82954.1"
                     /translation="MKRFVDLGDFSRDELLSLLELARRLEARPEPQALAGKILGLLFF
                     NPSLRTLASFQAAMARLGGSSFVINPGHGSWVLETRRGAVMDGAAAEHVREAIPALGS
                     YCDALGIRVFAEQRDLASDLAEITFNSMAELAEKPLINLESALNHPCQALADWKTMDD
                     LGVPRTGRFVLSWAWHPRALPLAVPAAAVHMACLRGMEVIVLRPEGFALPDPVMERAQ
                     RAAVHSGGTLRETSDRAEALAGADILYVKEWGATACYGDAAGDARLRSTLSSWCVRND
                     WFARTAPECRVMHCLPVRRNTAIADEVLDGPRSVVQREAANRLTVQTAVLHELLKS"
     gene            1750..>2285
                     /gene="argB"
                     /locus_tag="VM713_01440"
     CDS             1750..>2285
                     /gene="argB"
                     /locus_tag="VM713_01440"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HVH82955.1"
                     /translation="MIMHRGDQSLAIRALRSAAPYIRMYKGKTFVVKAGGGVFAEADA
                     TRSLVEQIGILHYFGVRVVLVHGGGPQLTELSAALGVPTQMVEGRRVTDRRSIEVTAM
                     VLNGLINTRVLAMCRDLDIDAVGISGVDAGLVRAQKRPPVRLSADGDPVDFGYVGDID
                     TVDTTVLRKLLDNGLMPVV"
BASE COUNT          330 a          810 c          795 g          350 t
ORIGIN      
        1 gtcacctggg gcgcgcaggc caaatggtac ggatttccgc gcacgccctt cgacgtgagc
       61 ttcattgcgc gcggcgcgac ggcgagcatc gccgagcacg tcaccaccga taccgcggca
      121 gtgatcgtcg agccggtgca gggactcgcc ggcgcagtgg cgctcggcgc gccgtttctg
      181 gcggcgctgc gcgcacgctg cgacgaggtc ggggcgctcc tcatcttcga cgaggtgcaa
      241 tgcggcatgg gccgcaccgg cgagcccttc gccgccaacc tctacggcgt gcggcccgac
      301 atgctgacca ccgccaaggc gctcggcaac ggcttcccgg tcgccgcgct catgatgtcg
      361 ccgctggtgt cggcctcact caagaccgaa gcgctcggta ccaccttcgg tggcgggccg
      421 atggcttgcg cggcggtaat tgcggtgctc gcggccatcc gcgagcagca cctcctcgag
      481 cgcgtgcggc gcatcggcgc ctacatccgc acgagctgca tcacgggccc ggtcacggcg
      541 gcttccgggg cggggctcct ggtcgggttg cgcaccacgc ggccggcgaa ggaaatccag
      601 ggcgagctcc tcgaatgcgg catcctcacc ggcacctcga gcgacccgca ggtcctgcgg
      661 ctgctgccgc cctacatcct cgaggaggag catgtcgacc tgctgcgcga tgcgctcaag
      721 gatctcgagg catgaagcgc ttcgtcgatc tcggggattt cagccgcgac gagctgctct
      781 cgctcctcga gctggcgcgg cggctcgagg cgcgacccga gccgcaggcg ctggccggga
      841 agatcctcgg gctgctgttc ttcaacccct cgctgcgcac gctcgcctcg ttccaggcgg
      901 cgatggcgcg cctcggcggc agctccttcg tcatcaatcc ggggcacggc agctgggtgc
      961 tcgagacgcg ccgcggcgca gtcatggacg gcgcggcggc cgagcacgtg cgcgaggcga
     1021 tcccggcgct cggctcttac tgcgatgcgc tcggcatccg cgtgttcgcc gagcagcgtg
     1081 atctcgccag cgatctcgcc gagatcacct tcaacagcat ggcggagctg gccgagaagc
     1141 cgctcatcaa cctcgaatcg gcgctcaacc atccctgcca ggcgctcgcg gactggaaga
     1201 ccatggacga tctcggcgtg ccgcgcaccg gccgcttcgt gctctcctgg gcctggcatc
     1261 cgcgcgcgct gccgctggcg gtaccggccg cggcggtgca catggcctgc ctgcgcggca
     1321 tggaggtgat cgtgttgcgc ccggaaggct tcgcgctgcc cgatccggtg atggagcgcg
     1381 cgcagcgcgc ggccgtccat tccggcggga ccctgcgcga gaccagcgac cgcgccgagg
     1441 cgctcgccgg tgccgacatt ctctacgtca aggaatgggg cgcgaccgcc tgctacggcg
     1501 atgcggcggg cgatgcgcgg ctgcgcagca cgctcagcag ctggtgcgtg cgcaacgact
     1561 ggttcgcgcg caccgcgccc gagtgccgcg tgatgcactg cctgccggtg cggcgcaaca
     1621 ccgcgatcgc cgacgaggtg ctcgacggcc cgcgcagcgt cgtgcaacgc gaagctgcca
     1681 accgcctcac cgtgcagacg gcggtgctcc acgagttact gaagtcctga agatcaggga
     1741 agccgcatca tgatcatgca tcgaggcgat cagagtctcg caatccgcgc cttgcggagc
     1801 gccgccccct acatccgcat gtacaagggc aagaccttcg tggtgaaagc cggcggcggc
     1861 gtgttcgccg aggccgacgc gacgcgctcg ctggtcgagc agatcgggat cctgcactac
     1921 ttcggcgtgc gcgtggtact ggttcacggc ggcggaccgc agctcaccga gctctcggcc
     1981 gcgctcggcg tgccgaccca gatggtcgag ggccgccgcg tgaccgatcg gcgctcgatc
     2041 gaggtcacgg cgatggtgct gaacggcctc atcaataccc gcgtgcttgc catgtgccgc
     2101 gatctcgaca tcgacgcggt cggcatcagc ggcgtggacg cgggcctggt acgcgcccag
     2161 aagcgcccgc cggtgcgctt gagcgccgac ggcgacccgg tcgacttcgg ctacgtcggc
     2221 gacatcgaca cggtcgacac cacggtgctg cgcaagctgc tcgacaacgg gctcatgccg
     2281 gtggt
//
LOCUS       DATLKL010000110         2453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_147788, whole genome shotgun sequence.
ACCESSION   DATLKL010000110 DATLKL010000000
VERSION     DATLKL010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147788"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VM713_01445"
     CDS             complement(<1..734)
                     /locus_tag="VM713_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVH82956.1"
                     /translation="MSRATRWIPGAAVLLAALVPSAAVGAAGTATEEVAVTRDVMITM
                     RDGVRLAADVYVPAQGGVATEGRFPALLMRTPYSKAVRAPPFANFFAARGYVVVVEDV
                     RGRYQSEGRWRPLYDDGADGYDTAQWIGRQGWCDGGIGTLGTSYEGATQQALAIAGAP
                     FLKTMVPLFSMSDVGRYGVRHNGAFELRWFNWVFTLGNATGEQSLVAAVRAASDPMAA
                     PALAELGNRVPEYVSALPLRAGTTPL"
     gene            complement(748..1533)
                     /locus_tag="VM713_01450"
     CDS             complement(748..1533)
                     /locus_tag="VM713_01450"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HVH82957.1"
                     /translation="MSIDEPLVKRSQDARGVVTLTLNRPQAYNALSEALLSALQGELD
                     SIAADESVRVVVLAAAGKAFCAGHDLREMRARPSQEYYERLFAQCSRMMLSLTRLPVP
                     VIARVQGIATAAGCQLVAQCDLAVASSEARFAVSGVNLGLFCSTPGVPLSRNVSRKRA
                     FEMLVTGEFINAQEALARGLVNRVVEPAALEAEAERLVASIVAKPRVAVAMGKELFYR
                     QAELGLASAYEAAGETMACNMMEEAALEGVQAFLEKRPPRWGS"
     gene            complement(1520..>2453)
                     /locus_tag="VM713_01455"
     CDS             complement(1520..>2453)
                     /locus_tag="VM713_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVH82958.1"
                     /translation="FAVVSLFVGTLGVANYRGVAAGTLVSNVSVVAKLVPLALVAGVG
                     VVYLTAHHALAPAAARGGIDAWLQAVLLLIFAYGGYEAALFPMGEARDPRHDVPFALF
                     AALLIVAILYTVLQVVVVGALPDAAHSERPLADAARMMMGQGGAALISIGALIAVYGY
                     LSANLLGSPRATFALAERGDFPARFAAIHPRFHTPYFSITVYAALVWAFALFGSFTWN
                     LTLSALARLFYYGAVCAAVPVLRVKQPGAAAFRLRGGPVVPALGVLICLTLLTRVNFS
                     KSLIVAAVILTATVNWLLVRGRGTMAPEDAHEYR"
BASE COUNT          414 a          880 c          844 g          315 t
ORIGIN      
        1 tggagtgggg tcgtcccggc gcgcaggggc agcgcgctga cgtactcggg tacacgatta
       61 cccagctcgg cgagcgctgg cgccgccatc gggtcgctgg cggcgcgcac tgccgccacg
      121 agactctgct cgcccgtagc attgccgagc gtaaacaccc aattgaacca gcgcagctcg
      181 aaagcgccgt tgtgccgcac gccgtagcgg ccgacgtcgg acatcgagaa cagcgggacc
      241 atcgtcttga ggaacggcgc gccggcgatc gcgagcgcct gttgcgtggc gccttcgtag
      301 gaggttccga gcgtaccgat gccgccgtcg caccagccct ggcggccgat ccactgggcg
      361 gtgtcgtagc cgtcggcacc gtcgtcgtac agcggccgcc agcggccctc ggactgatag
      421 cggccgcgca cgtcctcgac caccaccacg taaccgcgcg cggcaaagaa attggcaaac
      481 ggcggcgcgc gcaccgcctt gctgtagggc gtgcgcatga gaagcgcggg gaaacggccc
      541 tcggtcgcaa cgccgccctg ggccggaacg tagacatccg cagcgagccg cacgccgtca
      601 cgcatcgtga tcatcacgtc gcgcgtcacg gccacttcct cggtcgcggt gcccgcggcg
      661 cccacggccg cgctcggcac cagggccgcg agcagcaccg ccgcgccggg gatccaccgg
      721 gtcgcgcggc tcatgcgcag cgccgcctca cgatccccag cgtggcgggc gcttctcgag
      781 gaaggcctgc acgccctcga gcgcggcctc ctccatcatg ttgcacgcca tggtctcgcc
      841 ggcggcctcg taggcgctcg cgaggccgag ctccgcctgg cgataaaaga gctccttgcc
      901 catggcgacc gcgacgcgtg gcttggcgac gatgctcgcc accagccgct cggcctccgc
      961 ctcgagcgcg gcgggctcga ccacccggtt tacgaggccg cgcgcgagcg cttcctgcgc
     1021 attgatgaac tcgcccgtta ccagcatctc gaaggcgcgc ttgcgcgata cgttgcggga
     1081 cagcggcacg ccgggggtcg agcagaagag cccgaggttg accccgctca ccgcgaagcg
     1141 cgcctcgctc gaggcgacgg ccagatcaca ctgcgccacc agctgacagc ccgccgcggt
     1201 cgcgatgccc tgcacacgcg cgatcaccgg gacgggcaag cgggtgaggc tcagcatcat
     1261 gcggctgcac tgtgcgaaca gccgctcgta gtactcctgc gagggccgcg cgcgcatctc
     1321 gcgcaggtcg tgtccggcgc agaaggcctt gcccgcggcc gccagcacca cgacgcgcac
     1381 cgactcgtcg gcggcgatcg aatcgagctc gccctgcagc gcgctgagca gcgcctcgga
     1441 cagggcgttg taggcctgcg ggcggttgag ggtcagggtg accaccccac gcgcatcctg
     1501 actgcgtttc accagcggct catcgatact catgtgcgtc ctccggtgcc attgtgccgc
     1561 ggcctcgcac cagcagccag ttcacggtgg cggtcaggat caccgccgcc acgatcagcg
     1621 acttgctgaa gttgacgcgt gtcaggagcg tgaggcagat cagcaccccg agcgcgggca
     1681 cgaccggccc gccgcgtagc cggaaggctg cggcaccagg ctgcttcacc cgcaacaccg
     1741 gcaccgcggc gcacacggcg ccatagtaga agagccgcgc cagcgccgac agcgtgaggt
     1801 tccaggtgaa actgccgaag agcgcaaagg cccacaccag cgccgcgtac accgtgatcg
     1861 agaaatacgg cgtatggaag cgcggatgga tcgcggcgaa gcgcgccggg aaatctccgc
     1921 gctcggcgag cgcaaacgtg gcacggggac tgcccagcag gttcgcgctg agatacccgt
     1981 ataccgcgat gagcgcgccg atcgagatca gcgccgcgcc gccctgcccc atcatcatcc
     2041 gggccgcgtc ggccagcggg cgctcgctgt gcgcggcatc gggcagagcg ccgacgacca
     2101 cgacctgcag caccgtgtag aggattgcga cgatgagtag tgccgcgaag agcgcgaagg
     2161 ggacatcgtg gcgcgggtcc ctggcctctc ccatgggaaa gagcgccgcc tcgtagccgc
     2221 cgtaggcgaa gatcaggagg agcaccgcct gcagccaggc atcgatgccg ccgcgggcgg
     2281 cggccggcgc gagtgcgtgg tgggcggtga gatagacgac gccgacgccc gccaccaggg
     2341 cgagcggcac gagcttggcg accaccgaca cgttactcac cagcgtgccc gcggcgacgc
     2401 cgcgatagtt cgccacgccg agtgttccga cgaacagcga taccaccgcg aag
//
LOCUS       DATLKL010000111         2311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_147810, whole genome shotgun sequence.
ACCESSION   DATLKL010000111 DATLKL010000000
VERSION     DATLKL010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147810"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /gene="aceE"
                     /locus_tag="VM713_01460"
     CDS             <1..1514
                     /gene="aceE"
                     /locus_tag="VM713_01460"
                     /inference="COORDINATES: protein motif:HMM:NF036501.4"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HVH82959.1"
                     /translation="TVILAKTVKGFGLGKGGEGQMIAHQQKKMGEEDLKVFRDRFNIP
                     VADDELMQLPFRKPEENAEEAAYLREKRAVLGGYLPQRRHDAPPLVIPLLEAFSSILE
                     GTGEREISTTMAFVRILTILLKDKNIGRNIVPIVPDEARTFGMEGLFRQIGIYSSVGQ
                     LYTPQDAETLMSYREDKKGQMLEEGINEAGSLCSWIAAGTAYSNHGINMVPFYIFYSM
                     FGFQRVGDFIWAAGDIQARGFLLGATAGRTTLAGEGLQHQDGHSQLVASTVPNCIAYD
                     PAYAYELAVIIRDGLRRMYAERENIFYYLTVMNENYVQPAMPPGVEQGILNGAYLLQI
                     GGRGKVRATLLGSGTILRECLAAAKILEDDYGIPADVLSVTSFSELRREALECERWNL
                     LHPDRAPRIPYIRQLLKDRDGPVVAATDYMRTVPDQIRQWVGGRYITLGTDGFGRSDS
                     RAALRSFFEVDRNHIAIAALKALADEKRVDSKTVSGAIKALGVDPERPVPWKV"
     gene            1528..>2311
                     /locus_tag="VM713_01465"
     CDS             1528..>2311
                     /locus_tag="VM713_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVH82960.1"
                     /translation="MSAAPLLEVRVPDMGNFKDVAIIDVLVKPGESVEVDTPLVTLES
                     DKATMDVPSTAGGVIEKLHVAKGGTVSTGDLIATVRAAAAGAAASTSGRPAEVPPPPA
                     PPAALTPRASVPAPAAAPAAAPAAAPPAASPRPAAAVAGAERSGKLVPFSQLGTPTSF
                     RPDLPAIDEPGFSRAHAGPSVRLLARELGVDLARVTGTGFKGRITHEDVKAFVKQALT
                     APAPAAAAPAATARPALPALPVVDYSQFGPVETQPLTRIQKIS"
BASE COUNT          375 a          814 c          774 g          348 t
ORIGIN      
        1 cgacggtgat tctcgccaag acggtcaagg gctttggcct cggcaagggc ggcgaggggc
       61 agatgatcgc ccaccagcag aagaagatgg gcgaagagga cctcaaggtg tttcgcgacc
      121 gtttcaacat tccggtcgcg gatgacgagc tcatgcagct gccgttccgc aagcccgagg
      181 agaatgcgga ggaggcggct tacctgcgcg agaagcgcgc cgtgctcggc ggctacctgc
      241 cgcagcggcg ccacgacgcg ccgccgctgg tgatcccgct gctcgaggcc ttctcctcga
      301 tcctcgaagg gaccggcgag cgcgagattt ccaccacgat ggccttcgtg cgcatcctca
      361 cgatcctcct caaggacaag aacatcggcc gcaacatcgt gccgatcgtg cccgacgaag
      421 cgcgtacctt cggtatggag gggctgttcc ggcagatcgg catttactcc tccgtcggcc
      481 agctgtacac gccgcaggac gccgagaccc tcatgtccta ccgcgaggac aagaagggac
      541 agatgctcga ggaaggcatc aacgaggcgg gctcgctgtg ctcgtggatc gccgcgggca
      601 ccgcctacag caaccacggc atcaacatgg tgccgttcta catcttctac tcgatgttcg
      661 gcttccagcg cgtcggcgac ttcatctggg cggcgggcga tatccaggcg cgcggcttcc
      721 tcctcggcgc tacggccggg cgcaccacgc tcgcgggcga gggtctgcag caccaggacg
      781 gccacagcca gctggtcgcg agcacggtgc cgaactgcat cgcctacgac ccggcgtacg
      841 cctacgagct cgccgtcatc attcgcgacg ggttgcggcg catgtatgcc gagcgggaga
      901 acattttcta ttacctcacc gtgatgaacg agaactacgt gcagccggcg atgccgccgg
      961 gagtggagca ggggatcctc aacggcgcct accttctgca gatcggcgga cgcggcaagg
     1021 tgcgcgcgac gctgctcggc tcgggcacga tcctgcgcga gtgcctggcg gccgcgaaga
     1081 ttctcgagga cgattacggc atccccgccg acgtgctctc ggtgacgagc ttcagcgagc
     1141 tgcgacgcga ggcgctcgag tgcgagcgct ggaacctgct gcatccggac cgtgcgccgc
     1201 gcattcccta catccgccag ctgctgaagg accgcgacgg cccggtggtc gccgcgaccg
     1261 actacatgcg caccgtgccc gatcagatcc gtcagtgggt cgggggccgc tacatcacgc
     1321 tcggcaccga cggcttcggc cgctccgact cgcgcgcggc gctgcgtagc ttcttcgagg
     1381 tggatcgcaa tcacatcgcc atcgcggccc tgaaggcact ggcggacgag aagcgcgtgg
     1441 acagcaagac ggtgagcgga gcgatcaagg ccctcggcgt cgaccccgag cggccggtgc
     1501 cgtggaaagt ctgagggcgt tccggcgatg agcgcggccc cgctgctgga agtgcgcgtg
     1561 cccgacatgg gcaacttcaa ggacgtcgcc atcatcgacg tgctggtgaa gcccggtgag
     1621 agtgtcgagg tcgatacacc cctcgtcacg ctcgagtcgg acaaggccac gatggatgtt
     1681 ccttccacgg ccggcggggt catcgagaag ctccacgtgg cgaaaggcgg caccgtctcg
     1741 acgggcgatc tgatcgccac cgtgcgcgcc gccgccgccg gcgcggcggc cagcacgagt
     1801 ggcaggcccg cagaggtacc gccgcctccc gcgccgccag ctgcgttgac gcccagggca
     1861 tcggtcccgg ctccggctgc tgcgccagcg gcggcgcctg cagccgcccc gcccgcagcg
     1921 tcgccgcgac cagcagccgc ggtcgcaggc gccgagcgct ccggcaagct cgtgcccttc
     1981 tcgcagctcg ggaccccgac ctcattccgt cccgacctgc cggccatcga cgagcctgga
     2041 ttttcgcgcg cccacgcggg tccctcggtg cgcctcctcg cccgcgagct cggcgtcgat
     2101 ctcgcccgcg tcaccggcac gggtttcaag ggccgcatca cgcacgagga cgtcaaggcc
     2161 ttcgtcaagc aggcgctcac cgcgccggcc ccggcggcgg cggcgcctgc cgccacggcg
     2221 cgtccggcct tgccggctct gccggtggtc gactactcgc agttcggacc cgtcgagacg
     2281 cagccgctca cgcgcatcca gaagatctcg g
//
LOCUS       DATLKL010000112         1710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_181678, whole genome shotgun sequence.
ACCESSION   DATLKL010000112 DATLKL010000000
VERSION     DATLKL010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181678"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VM713_01470"
     CDS             complement(<1..533)
                     /locus_tag="VM713_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HVH82961.1"
                     /translation="MPEVGAGGQARLAAARVAVVGAGGLGCAVLEYLTAAGVGHLTIV
                     DHDSVEESNLHRQPLYRMSDLGSRKVEAARAALGALNPEVRIEALAERVTPANAPLLA
                     AAADIVVDAADSFAATYVLSDSCVRAGKPLVSASVLGLSGYVGAFCGGAPSYRAVFPE
                     LPRQAGSCAESGVLGTAV"
     gene            complement(559..1161)
                     /locus_tag="VM713_01475"
     CDS             complement(559..1161)
                     /locus_tag="VM713_01475"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HVH82962.1"
                     /translation="MRLPRVYPIVDSAAWIGRLAPLGVRLVQLRIKERAADEVRAEVR
                     TARALCAAAGMQLIVNDYWQIALEEGCDFVHLGQGDLAAADLPRLRRAGLRLGISTHD
                     HEELERALGAQPDYVALGPIYPTLLKVMPWQPQGLERLGEWKRRIGALPLVAIGGLTV
                     ARLAGVFAAGADVAAVVTDTIRHRDPEARARQWLAAASVA"
     gene            complement(1158..>1710)
                     /gene="thiG"
                     /locus_tag="VM713_01480"
     CDS             complement(1158..>1710)
                     /gene="thiG"
                     /locus_tag="VM713_01480"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="functions in thiamine (vitamin B1) biosynthesis; in
                     Bacillus subtilis this enzyme catalyzes the formation of
                     thiazole from dehydroxyglycine and
                     1-deoxy-D-xylulose-5-phosphate and ThiS-thiocarboxylate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HVH82963.1"
                     /translation="TVKEAVTTAHMAREVFGTSWVKLEVIGEDDTLQPDVFGLSEAAR
                     VLCAEGFEVFPYTTEDLVVAERLIAAGCRVLMPWGSPIGSGRGLNNLFGLKALRAHFP
                     AVPLIVDAGLGVPSHAAQAMELGFDAVLINTAVAQATDPVAMAAAFAQAVEAGRAARG
                     AGPIEPRDFAAPSTPVVGQAFLV"
BASE COUNT          260 a          620 c          608 g          222 t
ORIGIN      
        1 accgcggtgc cgagcacgcc ggattcggcg caggagcccg cctgccgtgg cagctcgggg
       61 aagaccgccc gatagctcgg cgcgccgccg cagaacgcgc cgacgtaccc cgacagcccg
      121 agcacggaag cgctcaccag aggtttgccg gcgcgcacgc agctatcgct caggacgtag
      181 gtcgcggcga agctgtcggc ggcatcgacg acgatgtcgg cggcggcggc cagcagcggc
      241 gcgttggcgg gcgtcacccg ctccgccagc gcctcgatgc gcacttcggg gttcaacgcg
      301 ccaagggcgg cgcgcgcggc ctcgaccttg cgcgagccga ggtcactcat gcgatagagc
      361 ggctggcgat gcagattcga ctcctcgacg gagtcgtgat cgacgatcgt gaggtgtcca
      421 acgcccgccg cggtcaggta ctcgagcacc gcgcagccga gcccgccggc gccgacgacg
      481 gcgacccgcg ccgcggcgag ccgtgcctgg ccgcccgcgc cgacctcggg caacacgact
      541 ggcgagagta gcgattgctc atgccacgct tgccgccgcg agccactggc gcgcgcgcgc
      601 ctcgggatcg cgatgtcgga tggtgtcggt cacgaccgcc gccacgtcgg cacctgcggc
      661 gaacacgccc gcgaggcgcg ccacggtgag gccgccgatg gcgaccagcg ggagcgcgcc
      721 gatgcgccgt ttccattccc cgaggcgctc gaggccctgg ggctgccagg gcatgacctt
      781 cagcagcgtc gggtagatgg gaccgagagc cacgtagtcg ggctgcgcgc cgagcgcgcg
      841 ctcgagctct tcgtggtcgt gggtgctgat gccgagccgc agccctgcac ggcgcagtct
      901 cgggaggtcg gctgcggcga gatcgccctg gccgaggtgc acgaagtcac agccctcctc
      961 gagggcgatc tgccagtagt cgttgacgat gagctgcata ccggccgccg cgcacagcgc
     1021 gcgcgcggtg cgcacttcgg cgcgcacctc gtccgccgcc cgttccttga tgcgcagctg
     1081 cacgaggcgc acgccgagcg gcgcgagccg cccgatccat gcggcgctgt cgacgatcgg
     1141 atagacgcgc ggcagcctca tacgagaaac gcctggccga ccactggggt tgagggcgcg
     1201 gcgaaatcgc gcggctcgat gggacctgcg ccgcgcgcgg cacgcccggc ctcgaccgcc
     1261 tgtgcgaacg ccgccgccat cgccaccgga tctgtcgcct gcgcgactgc cgtattgatc
     1321 agcacggcgt cgaagccgag ctccatcgcc tgcgcggcgt gcgaggggac gccgaggccg
     1381 gcgtcgacga tgagcggcac ggcggggaaa tgcgcgcgca gcgccttgag accgaaaagg
     1441 ttgttcagcc cgcgcccgga gccgatcggc gagccccagg gcatcagcac ccggcagccg
     1501 gccgcgatca gccgctcggc gacgaccagg tcctccgtcg tgtagggaaa cacctcgaac
     1561 ccttccgcac acagaacacg cgcggcttcg ctgagtccga atacgtcggg ctgcagcgta
     1621 tcgtcctcgc cgatgacctc gagcttcacc cacgacgtgc cgaacacctc gcgtgccatg
     1681 tgggcggtcg tgaccgcttc cttcactgtg
//
LOCUS       DATLKL010000113         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_125237, whole genome shotgun sequence.
ACCESSION   DATLKL010000113 DATLKL010000000
VERSION     DATLKL010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125237"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VM713_01485"
     CDS             <1..1024
                     /locus_tag="VM713_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HVH82964.1"
                     /translation="IAPDVIHSEYCGWGSRMRIFGFPSADTQWSVVGGLKERVEREFD
                     GQYVAGQTRTPAYSWSVEAIYDRSGVPRFYGIGNHSLVESQTNYLDNQARLDIQVGRN
                     FNPHLQLAYLARLRYVDVLPGVLPTVPSIETRFPGLTGIGAEHELQHRLILTYDTRDS
                     AIIPSSGARFSLYGGFVSRALGSSVSYSYLGIEMRQYLPLGHDVTLAWHAAARFMTSA
                     GGAPFWALSSLGGERSIPGEYEPLRSEGADRWVDRDLVAAGAELRKRVLGFDAFGTRV
                     SLELAPFVDAGRVFSDSDSSLGGHLHRAIGLGFRGVASPYVVGYVDFGYGQGRGAVFS
                     GINYPF"
     gene            1049..1122
                     /locus_tag="VM713_01490"
     tRNA            1049..1122
                     /locus_tag="VM713_01490"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1081..1083,aa:Gly,seq:ccc)
     gene            complement(1236..1682)
                     /locus_tag="VM713_01495"
     CDS             complement(1236..1682)
                     /locus_tag="VM713_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVH82965.1"
                     /translation="MRTLTISQVARQFGLRSSALRYYEKIGILPAVERTSGQRRYAEA
                     SLRRLAVIQRARDAGFALEDIRELFAGFRPGVPASQRWQQVSQRKLSEIEESIARLSA
                     MKDLLRRMSNCKCDALDECGAAILRTIKSQPQRELASSRRITSLRA"
     gene            complement(1704..>2094)
                     /locus_tag="VM713_01500"
     CDS             complement(1704..>2094)
                     /locus_tag="VM713_01500"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HVH82966.1"
                     /translation="ALDGRRIRLSDFRDRVVLVNFWATWCAPCRVEMPWLREFDARYG
                     SQGLTILGISVDDGGRERVERFVRQLRVEYPILLSDGNVDLRYGGVRFLPQTFFVGRD
                     GKITRRVYGIRTHEDFEAEIRGALGLP"
BASE COUNT          359 a          719 c          647 g          369 t
ORIGIN      
        1 catcgcccca gacgtgatcc acagcgagta ctgcggctgg ggcagccgca tgcgtatctt
       61 cggtttcccc tccgcggata ctcagtggtc ggtggtcggc gggctcaagg agcgcgtcga
      121 gcgcgagttc gacggccagt acgttgccgg ccagacgcgc acccctgcct acagctggtc
      181 ggtggaggcg atctacgatc gcagcggcgt gccgcgtttc tacggcatcg gcaaccactc
      241 gctggtcgaa agccagacca actatctcga caaccaggcg cggctcgaca tccaggtcgg
      301 gcgcaatttc aatccgcacc tgcagctcgc ctacctcgcg cggctacgct acgtcgacgt
      361 gctgccgggc gtgctgccga cggtgccgtc gatcgagacc cgtttccccg ggctgaccgg
      421 gatcggcgcc gagcacgagc tgcagcaccg gctgatcctc acctacgaca cgcgcgactc
      481 ggcgatcatt ccgagctcgg gggcgcgctt cagtctttac ggaggcttcg tgagccgcgc
      541 cctcggcagc tcggtctcct acagctacct cggcatcgag atgcgccaat acctgccgct
      601 cggccacgac gtcacgctcg cctggcacgc cgcggcgcgc ttcatgactt ccgcgggcgg
      661 cgcacctttc tgggcgctct ccagcctcgg gggcgaacgc agcatcccgg gcgagtacga
      721 gccgctgcgc tcggaaggcg ccgaccgctg ggtcgaccgc gatctcgtgg ccgctggcgc
      781 cgagctgcgc aagcgggtgc tcggattcga tgccttcggc acgcgcgtca gcctcgagct
      841 cgcgccgttc gtggatgccg gcagggtgtt cagcgattcc gactcgagcc ttggcgggca
      901 cttgcatcgc gccatcgggc tcggctttcg cggagtcgcc agcccgtacg tcgtcggcta
      961 cgtcgatttc ggctacggcc agggaagggg cgcggtgttc agcgggatca actacccgtt
     1021 ctgactaggg taagatcccg actccggcgc gggtgtagtt caatggtaga actgcagctt
     1081 cccaagctgc taacgtgggt tcgattccca tcacccgctc caattccagc caattcgggc
     1141 cagcgatagc caagacgatc cgtcaccgct tgagtgtcgc catgctcgcc ctgatcgccg
     1201 gtcccgccgc gacgcgaagt cccccgagtc cgccctcagg ccctcagcga tgtaatccta
     1261 cgactgcttg cgagctcacg ctgcggctgc gatttgatcg tacgcaaaat ggccgcaccg
     1321 cactcatcca gcgcatcgca cttgcagttg ctcatacgcc tgagcaggtc cttcattgcc
     1381 gagagtcgcg caatgctctc ctcgatctcc gacagtttgc gctgtgacac ctgctgccag
     1441 cgctgcgatg ccggtacacc gggacgaaag cccgcaaaca gctcgcgaat gtcctcgagc
     1501 gcgaaacccg catcacgcgc acgctgaatg accgcgaggc ggcgcaggct ggcttcagca
     1561 tagcggcgct gcccgctggt gcgctcgaca gccggcagga tcccgatttt ctcgtagtag
     1621 cgcagcgctg agcttctcaa gccgaactgc ctggcgacct gcgaaatggt tagggttcgc
     1681 atgcggtgag tgtacgccgt gaactagggt agccccaagg cgccgcggat ctcggcctcg
     1741 aagtcctcat gcgtgcggat cccatacacg cgccgcgtga ttttgccatc gcggcccacg
     1801 aagaatgtct gcggcaggaa tcgcacgccg ccatagcgca aatcgacatt tccgtcactc
     1861 aacagaatcg gatactccac gcgcagctgg cggacgaagc gctcgactcg ctcgcgaccg
     1921 ccgtcatcga ccgaaatacc gagaatcgtt agtccctgtg aaccgtagcg ggcgtcgaac
     1981 tccctgagcc acggcatttc cacgcggcag ggcgcacacc aagtggccca gaagttgacg
     2041 agcactacgc gatcgcggaa gtcgctgaga cggatgcggc gcccgtcgag cgcg
//
LOCUS       DATLKL010000114         1853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_181691, whole genome shotgun sequence.
ACCESSION   DATLKL010000114 DATLKL010000000
VERSION     DATLKL010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181691"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VM713_01505"
     CDS             complement(<1..1250)
                     /locus_tag="VM713_01505"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82967.1"
                     /translation="MNDLSRAAPVAGPTATVDQQNPWLGLASFTEETRAYFFGRDEEV
                     AELARRVQRKLLTILFGQSGLGKTSILRAGLVPRLRGQGYCPVYVRIDYSRDSPEPAE
                     QIKRAIQMNARHSGQWTQAGVAVEGESLWEFLHHRDDVLRDEAGRTLIPLLIFDQFEE
                     LFTLAQSDDFGRARAARFVAELADLVENRPPQEFEAKLEEDESASERFDFARSDYRVL
                     IALREDYLAPLEGLKGSIPSISQNRLRLAPMTGTQALAAVLEPGRGLVTPEVAAAIVR
                     FVAGGAELSNAVVEPSLLSLICRELNDARIAQGRSEISLDLLAGSHASILSNFYERAL
                     ADQPPAVRRIIEDDLLTESGFRENVAEERLVSRFTEAGAAPGTLALLVNRRLLRIEER
                     LDVRRVELTHDVLCGVVKASRDRR"
     gene            complement(1247..>1853)
                     /locus_tag="VM713_01510"
     CDS             complement(1247..>1853)
                     /locus_tag="VM713_01510"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HVH82968.1"
                     /translation="SLDNRLVSFLQQVSVRTRIYVGAERFVAELHARWQARRKPTGSP
                     AAPTRFLPPAREMPDNAVFISYAREDLAAVQQIKAGLEAAGITTWFDMDRLEVGDDYD
                     RKIRGNIARCSYFIPVVSATTQRRLEGYFRREWSYAIDRARNMADGALFILPVSIDAI
                     DAAAALVPEKFKALHFTQLAGGAVPAEFAARLADFMRARSQ"
BASE COUNT          291 a          627 c          661 g          274 t
ORIGIN      
        1 tggcggcggt cgcggctcgc cttgacgacg ccgcacagca cgtcgtgggt gagctcgacg
       61 cggcgcacgt cgagccgctc ctcgatgcgc aacaggcgcc ggttgacgag cagcgcgagc
      121 gtgcccggcg ccgcgcccgc ctcggtgaag cggctcacca ggcgctcctc ggccacgttc
      181 tcgcggaagc ccgactcggt cagcagatcg tcctcgatga tgcgccgtac cgccggcggc
      241 tggtcagcga gcgcgcgctc gtagaaatta ctgagaatgc tggcgtgcga gccggcgagc
      301 aggtcgagag agatctcgct gcgtccctgc gcgatgcgtg cgtcgttcag ctcgcggcag
      361 atgagagaga ggagcgaggg ctcgaccacc gcgttcgaga gctcggcgcc gccggccacg
      421 aagcgcacga tcgcagccgc cacctcaggg gtgaccagcc cgcggcccgg ctcgagcacc
      481 gccgcgagcg cctgggtgcc ggtcatgggg gcgagcctca ggcggttctg ggaaatgctc
      541 gggatcgatc ccttcaggcc ctcgagcggg gcgagatagt cctcgcgcaa cgcgatcagc
      601 acgcgatagt cgctgcgcgc aaaatcgaag cgctcggagg cggactcgtc ttcctcgagc
      661 ttggcttcga actcctgcgg cggccggttc tcgaccaggt cggcgagctc ggccacgaaa
      721 cgcgcggccc gcgcgcgccc aaagtcgtcg ctctgggcaa gcgtgaacag ctcctcgaac
      781 tggtcgaata tgagcagcgg gatgagagta cggccggcct catcgcgcaa cacatcgtcg
      841 cggtggtgca ggaactccca gagcgattcg ccctcgaccg cgactcccgc ctgggtccac
      901 tgcccggaat gccgcgcgtt catctgaatg gcgcgcttga tctgctcggc cggctcgggc
      961 gaatcgcggc tgtagtcgat gcgcacgtac accgggcagt agccctgacc gcgcaggcgc
     1021 ggcaccagcc cggcgcgcag gatcgaggtc ttgccgagtc ccgactggcc gaagaggatc
     1081 gtgaggagct tgcgctgcac gcggcgcgcg agctcggcca cctcctcatc gcgcccgaag
     1141 aagtaggcgc gcgtctcctc ggtgaaggag gcgaggccca gccagggatt ctgctgatcg
     1201 acggtggcgg tcgggccggc taccggcgcc gcgcggctca ggtcgttcat tgcgagcgcg
     1261 cccgcatgaa gtccgccagg cgcgcggcga actccgccgg caccgcgccg cccgcgagct
     1321 gcgtgaagtg cagcgccttg aatttctcgg gcaccagcgc cgcggcggca tcgatggcat
     1381 cgatgctcac cggcaggatg aacagcgccc cgtcggccat gttgcgcgcg cgatcgatcg
     1441 cgtagctcca ctcacggcgg aaatagccct cgagccgccg ctgcgtggtg gccgagacta
     1501 ccgggatgaa ataagagcag cgggcgatgt tgccgcggat cttgcggtcg tagtcatcgc
     1561 cgacctcgag ccggtccatg tcgaaccagg tggtgatgcc ggcagcctcg agtcccgcct
     1621 tgatctgctg gaccgccgcc aggtcctcgc gtgcatagct gatgaagact gcgttgtcgg
     1681 gcatctcgcg cgccggcggc aaaaagcgtg tgggcgcggc gggcgagccc gtcggcttgc
     1741 ggcgcgcctg ccagcgcgcg tgcagctcgg cgacgaagcg ctcggcgccg acgtagatgc
     1801 gggtgcgcac gctcacctgc tgcaggaagg acacgagccg gttgtcgagg ctc
//
LOCUS       DATLKL010000115         2511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_23802, whole genome shotgun sequence.
ACCESSION   DATLKL010000115 DATLKL010000000
VERSION     DATLKL010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23802"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VM713_01515"
     CDS             complement(<1..1062)
                     /locus_tag="VM713_01515"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HVH82969.1"
                     /translation="MNAQSWHVVSESLPASRKVYRAGRLHPDLRVAMREIALHPSAGE
                     PPLTVYDPSGPYTDPAVTIDIEAGLGRLRERWIAARGDTEAYPGRRVQLIDNGLSSGA
                     KAAPEFPLRRPPVRARAGQAVTQLAYARAGIVTPEMEYVAIRENLGRERAAAGAARDG
                     NSWGALIPEYVTPEFVRAEIAAGRAILPANVNHPESEPMIIGRNFLVKINANIGNSAV
                     TSSTAEEVDKMVWAIRWGADTVMDLSTGRNIHTIREWILRNAPVPIGTVPIYQALEKV
                     GGVAEDHTWEIFRDTLIEQAEQGIDYFTIHAGVRLAYVPLTVARVTGIVSRGGSIMAK
                     WCLAHHRESFLYERFAEICE"
     regulatory      complement(1119..1242)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1378..1725)
                     /locus_tag="VM713_01520"
     CDS             complement(1378..1725)
                     /locus_tag="VM713_01520"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HVH82970.1"
                     /translation="MAQGTEGRLSHRGFASMNAERQRQIASMGGKAVPDEKRSFSQNR
                     RLAAEAGRKGGQSVPGSKRSFSQNRTLAAVAGRKGGQSVPDEKRSFSQHPELAAQAGR
                     KGGQASHGNGRPS"
     gene            complement(1873..>2511)
                     /locus_tag="VM713_01525"
     CDS             complement(1873..>2511)
                     /locus_tag="VM713_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82971.1"
                     /translation="ALTDESELDGRAARLIERALILDPDSGKALFFGATTAERRGELP
                     LARQRFAKLLALNPPDNLRPILEQHLRVIDAKLAAAPAAAVPAQKGAASAPAAASDAA
                     VVRVNVTLAPSIARVTDGAPLFVFVRDPQAAGPPLAVKRLDSHFPQTVALTPADSMIP
                     GRLFSAGQNVQVVARIARTGNPVGASGDPFGEVRYHVGKDGLVGLVIDRLTP"
BASE COUNT          377 a          853 c          868 g          413 t
ORIGIN      
        1 ctcgcagatc tccgcgaagc gctcgtagag gaaactctca cggtgatgcg cgagacacca
       61 cttcgccatg atcgagccgc cgcgcgagac gatgccggtg acgcgcgcga cggtgagcgg
      121 tacgtaggcg aggcgcacgc ccgcgtgaat ggtgaagtag tcgatcccct gctcggcctg
      181 ctcgatgagc gtgtcgcgga agatctccca ggtgtggtcc tcggccacgc cgccgacttt
      241 ctcgagcgcc tgatagatcg ggacggtgcc gatcggcacc ggagcgttgc gcaggatcca
      301 ctcgcggatg gtgtggatgt tgcggccggt cgagaggtcc atgaccgtgt cggcgcccca
      361 gcggatcgcc cacaccatct tgtcgacctc ctcggcggtc gaggaagtga ccgcggagtt
      421 gccgatgttg gcgttgatct tcaccaggaa attccgcccg atgatcatcg gctcggactc
      481 cgggtgattc acgttcgccg ggaggatggc gcggcccgcg gcgatctcgg cgcgcacgaa
      541 ctccggcgtc acgtattccg ggatgagcgc tccccaggag ttgccgtcgc gcgccgcacc
      601 tgcagccgcg cgctcgcgcc cgagattctc gcggatggcg acgtactcca tctccggcgt
      661 cacgatcccg gcacgcgcat aggcgagctg ggtcaccgcc tggccggcgc gggcgcgcac
      721 cggcggccgg cgcaggggaa attccggcgc ggccttggcg cccgagctga gtccgttgtc
      781 gatgagctgt acgcgccgcc cggggtaagc ctcggtatcg ccgcgtgccg cgatccagcg
      841 ctcgcgcagc cggccgagac ccgcttcgat gtcgatggtg acggcaggat cggtataagg
      901 gccggaagga tcgtagaccg tcagcggcgg ctcgccggcc gaggggtgga gcgcaatctc
      961 gcgcatcgcg acgcgcaggt ccgggtgcag acggcccgcg cgatacacct tgcgtgaggc
     1021 gggaagggat tcggaaacga cgtgccaact ctgcgcattc atcagacact cctggcgtaa
     1081 agccgcagaa gatccggcga atgctgccca tgggaaaggg agcggagggt tcgctttcct
     1141 acgccagcat taactggatc aggttcggag ggtcgccatg ctgcgcttcc tcgcgcgccg
     1201 atggcctctc agccccctgc cggggctccc ctagctgtac gcgcggaaag tagtacgagt
     1261 cgcggcgtcg gtcaatgccg cttgttgttt cgctcattgc accgcagcgg ggcgacgcgc
     1321 gtcgccccgc ctgactggaa agtccccggc gccgccagtg ctcctcagcg ccgacgctca
     1381 gctcgggcga ccgttcccgt gactcgcctg cccgccctta cggcccgcct gcgcggcgag
     1441 ctccggatgc tgcgaaaagc tgcgcttttc gtccggcacg ctctgcccgc ccttgcggcc
     1501 ggcgaccgcg gccagcgtcc ggttctgcga gaagctgcgt ttggatccgg gcacgctctg
     1561 cccgcctttg cgcccggcct cggcggccag gcggcgattc tgcgagaaac tgcgcttctc
     1621 atccggtacg gccttgcctc ccatgctggc gatctggcgt tgccgttccg cgttcatcga
     1681 ggcgaaacca cgatgcgaca gccgtccttc cgttccctga gccattgctc gtctcctccc
     1741 cgacccttgc ggccggcgca agcccgacaa aaagaaactc cctcttcccg ggtggcagca
     1801 tacccccggg ggcacgcgag gagaactagg ttaagcgggc ctcagcgcgc ggcgtagccg
     1861 ctgggtccgc cttcaagggg tgaggcggtc gatgacaaga cccacgaggc catccttgcc
     1921 tacgtggtag cgaacttcgc cgaaagggtc accgctcgcg cccaccgggt ttccggtacg
     1981 tgcgatgcgt gcgactacct gcacgttctg cccggccgag aaaaggcgcc ccgggatcat
     2041 cgagtccgcc ggcgtgagtg ctacggtctg cgggaagtgg ctgtcgagac gcttgacggc
     2101 cagcggcggg cccgccgctt gcggatcgcg cacgaacaca aagagcggtg ccccgtcggt
     2161 cacgcgtgcg atcgacgggg cgagcgtcac gttgacacgg acgaccgcgg cgtcgctcgc
     2221 ggccgctggc gccgacgccg cgcctttctg tgcgggcacc gcggcggcag gagcggcggc
     2281 gagcttcgcg tcgatcaccc tcaggtgctg ctcgagaatc ggccgcaggt tgtcgggagg
     2341 attcagcgcg aggagtttcg cgaagcgctg ccgcgcgagc ggcagctcgc cgcgccgctc
     2401 cgcggtggtt gcaccgaaga agagcgcctt gcctgagtcg ggatcgagga tcagcgcccg
     2461 ctcgatgaga cgcgcggcgc gtccgtcgag ctcgctctcg tcagtgagcg c
//
LOCUS       DATLKL010000116         2167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_57635, whole genome shotgun sequence.
ACCESSION   DATLKL010000116 DATLKL010000000
VERSION     DATLKL010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57635"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2000)
                     /locus_tag="VM713_01530"
     CDS             complement(<1..2000)
                     /locus_tag="VM713_01530"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HVH82972.1"
                     /translation="MTMPSTRRASGAPLNRLDGVQKVTGAATYAYEYPIEGATYVFPV
                     QSTIARGRVSSIDASAARALPGVLAVLSHENAPRLAPPRDDQDLAVFQSDAVAYRGQF
                     VAAVVAETLETARQAASMVAVRYEEQPHDVVLRADRGDLFTPEYVPQKPFGFYPADSA
                     HGDVEAALAAAPVSLDHTYTTPAEHHNPMEPHTTLAIWSDDGTGESVTVYDTCQGVSW
                     RRDDIAIAFGLSSERVRVIAPYVGGGFGSRGFTHPYAILAVMAARVVGRPAKLAQTRQ
                     QMFGPVGYRSPTIQHIRLGAGRDGRLTAIAHEVVAQTNALYEMTEPAAVPTRTMYATP
                     NFLTTHRLARLDLPANSWMRAPGESSGMFALESAMDELAVECGLDPIELRLRNEPAVD
                     PETGLPYSSRGLVACLREGAERFGWQPRDPTPGLRREGRWLIGTGVAASTYPAVQLPA
                     SARVAVDAAGRYRVSIAAVDIGTGAWTVLSQIAADALAVPVEQVQLEIGDSALPQAFP
                     AWGSTGTASWGWAITDAAQKLRARMRDEHGGAIPPEGLEADGGFAGNPAAQQFSMHAF
                     GAQFAEVRVNEDTGEVRVPRLLGVFAAGRIVNPKTARSQLLGGMTWGLSMALHEASVM
                     DPRFGDYVNHDFAEYHIASNADVGAIEVAFIDEEDPHVNPIGA"
BASE COUNT          323 a          758 c          739 g          347 t
ORIGIN      
        1 gcaccgatcg ggttgacgtg tggatcttcc tcgtcgatga aggcaacttc aattgcgcct
       61 acgtcagcgt tgctggcaat gtggtactcg gcgaagtcgt ggttgacgta gtcgccgaag
      121 cgcgggtcca tgacgctcgc ttcgtgcagc gccatcgaaa gtccccacgt catgccgccc
      181 agcagctgcg agcgggcggt cttcgggttg acgatgcgtc cggcggcgaa gacccccagc
      241 agccgcggca cccgcacctc gcccgtgtct tcattgacgc ggacctcggc gaactgcgca
      301 ccgaaggcat gcatcgagaa ctgctgcgcc gcggggtttc cggcaaagcc gccgtccgct
      361 tccagcccct caggcgggat ggcgcctcca tgctcgtcgc gcattcgcgc gcgcagcttc
      421 tgcgcggcat cggtgatcgc ccacccccag gaggcggtgc ccgtcgagcc ccaggccggg
      481 aacgcctgcg gcagggcgct gtcgccgatc tccagctgca cctgctccac aggcacggcc
      541 agcgcgtcgg ctgcgatctg ggacagcacg gtccaggcgc ccgtgccgat gtccacggca
      601 gcaatgctga cgcgatagcg tcccgccgcg tctacggcaa cacgcgcgct tgcgggcaac
      661 tgcacggctg gataggtcga ggcggccacg ccggtgccga tcagccagcg tccctcccgt
      721 cgcaggcccg gcgtcgggtc gcgcggctgc cagccgaagc gctcggcgcc ttcgcgcagg
      781 caggccacca atccgcggct gctgtagggg aggcctgttt ccggatccac cgcgggctcg
      841 ttgcgcaggc gcagctcgat cggatcgagg ccacactcca ccgccagctc atccatcgcc
      901 gactcgagcg cgaacatccc cgagctctcg cccggcgcgc gcatccacga gttcgccggc
      961 aggtcgagcc gcgccagccg gtgggtggtg aggaagttgg gcgtcgcgta catcgtccgt
     1021 gtcgggacgg cggccggctc ggtcatctcg tagagcgcgt tggtctgggc caccacctcg
     1081 tgcgcgatcg ccgtgaggcg gccgtcgcgc ccggcgccca gacgaatgtg ctggatggtc
     1141 ggcgagcggt accccaccgg cccgaacatc tgctgccgcg tctgcgccag cttcgccggc
     1201 cggcctacga cgcgcgcggc catgacggcg aggatggcat acgggtgggt aaatccccgg
     1261 gatccaaacc cgcctccaac gtagggcgcg atgacgcgta cgcgctcgga cgaaagtcca
     1321 aacgcaatgg cgatgtcgtc tcgtctccac gacaccccct ggcacgtgtc gtagacggtc
     1381 acgctctcgc cggtgccgtc gtcactccag atcgccaggg tcgtgtgcgg ctccatgggg
     1441 ttatggtgct cggcaggcgt tgtgtacgtg tggtcgagcg agacaggagc ggccgccagc
     1501 gctgcctcga cgtcgccgtg ggcgctatcc gctggataga atccgaacgg cttttgcggt
     1561 acgtattcgg gcgtgaagag atcgccgcgg tcggcgcgca gcacgacgtc gtgcggctgt
     1621 tcctcatacc gcacggcaac catgctcgct gcctgacgcg cggtctccag tgtctcggcc
     1681 acgacggcgg cgacgaactg cccgcgatag gccacggcat cggattggaa gacggccaga
     1741 tcctggtcgt cccgcggagg agcgagccgc ggggcattct cgtgcgagag cacggcgagg
     1801 actcccggca gcgcacgcgc ggcactcgcg tcgatcgagc tgactcggcc cctggcgatc
     1861 gtgctctgca ccgggaagac ataggtcgct ccttcgatcg gatactcgta ggcataggtt
     1921 gccgcgccgg tgaccttctg caccccgtcg agacggttga ggggcgcgcc gctggcacgc
     1981 ctggtagatg gcatcgtcat ggttgcactt cctcctctgt caggtcaagc agcgtgcgca
     2041 ccaggacgct cctggcgagc gggaccttga acgcgttgcc gggcaagggc agtgcctcgg
     2101 ccagcaccgc ctcggccgcg cgcccaaaca tctcctcggt ggccggggcg ccgcgcagca
     2161 ccgcctc
//
LOCUS       DATLKL010000117         3110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_215385, whole genome shotgun sequence.
ACCESSION   DATLKL010000117 DATLKL010000000
VERSION     DATLKL010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215385"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VM713_01535"
     CDS             complement(<1..570)
                     /locus_tag="VM713_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HVH82973.1"
                     /translation="MLLDLGGAHEPGAAGPSLELLRQILNLTPDVKLIVITEPDAREL
                     TVAAIGLGATDFYHKPFDAGVLSLVVRRALRIRELEEENWRLRERTGDMALEGIIGVS
                     DAMRSLCRAVEKVAPTNATVLLLGDSGTGKELLARALHRLSGRSHKPFVGINCAAIPE
                     TLLESELFGYEKGAFTGAAKRTAGKLESAE"
     gene            complement(477..725)
                     /locus_tag="VM713_01540"
     CDS             complement(477..725)
                     /locus_tag="VM713_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82974.1"
                     /translation="MSVANRRRKLLIVVNDRTLRRELESMFSDLEVTASRAGEDTLAI
                     VRRTEPTSCCSIWVVHMSRAPPGRASSCCARSSTSPPT"
     gene            830..1816
                     /locus_tag="VM713_01545"
     CDS             830..1816
                     /locus_tag="VM713_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HVH82975.1"
                     /translation="MTDAAETRVRQFRSALGAFATGVTIVTTRTRSGVDVGLTANSFN
                     SVSLDPPMVLWSLAKSSKSLPAFLESGYFAVHVLSASQEALSHTFATRGAEKFAGLSL
                     ERGRGDIPLLDGCSARFQCRTAVKYEGGDHLVFIGEVEAFDHFDRPPLVFHGGGYAAA
                     LAKPRAVPAPEAAEPDSSFSEDFLIFLVARAHFQLFLGLQRDLERHGLTEWQWFVLSI
                     LGVRDNRTVADLDRILWRTGTRVTYELVAGLAAAGLAQLHGSNDPSARVTLTDDGRRV
                     VIELVAAAKALESHAEGNLGFDEAQLLKQALRSIVRETDPGLPPLRSPTYTE"
     gene            1833..3014
                     /locus_tag="VM713_01550"
     CDS             1833..3014
                     /locus_tag="VM713_01550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVH82976.1"
                     /translation="MTEGHEAQFAAQLAAVRRITTEVAARHAADVDARSRFPRESLEA
                     LQAAQVLSAPVPAALGGAGLGMRELASLCSTLAQGCGASAMLLAMHYSQVACIVRHAA
                     ESAFFGEYLRELVARQYLLASMTSENGTFGETRTSICALERSGGHFKLEKDATTGSYC
                     EHADGILVTCRRAPDAAGTDQVLVLVRKGGYTLTQTTTWDALGMRGTCSPGFKLVSHG
                     PEEQIIPGSFADSSAQSMVPYSHILWSSLWWGIAADAVGRAAEFVRAAARRNPGTVPP
                     TASRLAEVSTRLQAMRHNWLALATEFDELAARGSDGMKELLTIGWALKLNNLKIGASQ
                     AAPQIVHEALQITGILGYKNDSPFSLGRHYRDVLSASLMISNDRILGKNASMLLIYKD
                     V"
BASE COUNT          492 a         1107 c         1009 g          502 t
ORIGIN      
        1 ctcggccgac tcgagcttgc cggcggtgcg cttggcagct ccggtgaagg cccccttttc
       61 gtatccaaag agctcgctct cgagcagcgt ctcggggatg gcggcgcagt tgatgccgac
      121 gaagggcttg tgcgagcggc ccgacagccg gtgcagcgcc cgcgccagca actccttgcc
      181 ggtgccgctg tcgccgagca gcagcacggt agcgttggtg ggtgcgacct tctcgaccgc
      241 gcggcacagg ctccgcatcg catcgctcac gccgatgatg ccctcgagcg ccatgtcacc
      301 ggtgcgctcg cgcagccgcc agttctcctc ctcgagctcg cgaatgcgca gcgcccgccg
      361 caccacgagc gacagcaccc cggcgtcaaa gggcttgtgg tagaaatcgg ttgcgccgag
      421 accgatggcc gcgaccgtga gctcgcgcgc atcgggctcg gtgatgacga tcagctttac
      481 gtcgggggtg aggttgagga tctggcgcag cagctcgagg ctcggcccgg cggcgcccgg
      541 ctcatgtgca ccacccagat cgagcagcac gacgtcggct ccgtgcgccg cacgatggcg
      601 agcgtgtcct cgcccgcacg gctcgcggtc acctcgaggt cggagaacat cgactcgagc
      661 tcgcgccgta gggttcgatc gttgacgacg atcagcagct tgcgccgccg gttggcgacc
      721 gacatccatt cgctcccgag ctgcgggtaa agactttaca atggattgta aagaagcgcc
      781 tgccgcctcg tcgtgaagcc cttaacaatt gcactacact aggagtgccg tgacggatgc
      841 cgccgagacc cgtgtgcgcc agttccgcag cgccctcggg gcgtttgcga ccggcgtcac
      901 aatcgtaacg acccgtaccc gctcgggcgt cgacgtcggg ctcaccgcca acagcttcaa
      961 ctccgtctcg ctcgacccgc cgatggtgct gtggagcctt gccaagagct ccaagagcct
     1021 cccggccttc ctcgagagcg gctatttcgc ggtacacgtg ctgtctgcat cccaggaagc
     1081 gctgtcgcat acctttgcca cgcggggcgc cgagaagttc gcgggtctct ccctcgagcg
     1141 cggccgcggt gacattccgc tcctcgacgg atgctcggcg cgcttccagt gccgcaccgc
     1201 cgtcaaatac gaagggggcg accacctggt gttcatcggc gaggtggaag ccttcgatca
     1261 tttcgaccgg ccgccgctcg tctttcacgg cggcggctat gccgccgccc tcgccaagcc
     1321 ccgcgccgtc ccggcgcccg aggcggccga gcccgactcg agcttcagcg aggatttcct
     1381 catctttctc gtggcgcgcg cgcacttcca gctgttcctc ggcctgcagc gcgatctcga
     1441 gcgccacggg ctcaccgaat ggcaatggtt cgtactgagc attctcggcg tgcgcgacaa
     1501 ccgcaccgtc gccgatctcg accggatcct gtggcgcacg ggtacgcgcg tgacctacga
     1561 gctggtggcg ggactggccg ccgcgggcct ggcacagctg cacggcagca acgacccgag
     1621 cgcccgcgtc acactcaccg acgacggtcg ccgcgtcgtg atcgagctgg tggcggccgc
     1681 caaggcgctc gagtcgcacg ccgagggcaa cctcggcttc gacgaggcgc agctgctcaa
     1741 gcaggcgttg cgcagcatcg tccgcgagac cgatccgggg ctgccgccgc tgcgctcgcc
     1801 cacctacacg gaatgacacg agggacacgc gcatgaccga gggccacgaa gcgcagttcg
     1861 ccgcgcagct cgccgccgtg cgccggatca ccaccgaggt cgctgcgcgc cacgctgccg
     1921 acgtcgacgc gcgcagccgc tttccgcgtg agagtctcga agccctgcag gccgcccaag
     1981 tcctctcggc accggtgccg gccgcgctcg gcggcgcggg actcgggatg cgcgagctcg
     2041 cctcgctgtg ctcgacgctc gcgcagggct gcggcgcgag cgcgatgttg ctcgcgatgc
     2101 attactccca ggtcgcgtgc atcgtgcggc atgcggccga gtccgcgttc ttcggcgaat
     2161 acctgcgtga gctcgtggcg cggcagtacc tgctcgcctc gatgacgtcc gagaacggca
     2221 ccttcggcga gacccgcacc agcatctgcg cgctcgagcg cagcggcggc cacttcaagc
     2281 tcgagaagga cgcgaccacg ggctcctact gcgagcacgc ggacgggatc ctggtgacct
     2341 gccgacgcgc acccgatgcg gcggggacgg atcaggtgct cgtcctggtg cgcaagggcg
     2401 gctacacgct cacccagacc accacctggg acgcgctcgg catgcgcggc acctgcagcc
     2461 ccggcttcaa actcgtctcg cacggcccgg aagagcagat catcccgggg tcgttcgccg
     2521 actcatcggc gcagagcatg gtgccctatt cgcacattct ctggtcgagc ctctggtggg
     2581 gcatcgcggc ggatgcggtg ggtcgcgccg cggagttcgt gcgcgccgcg gcgcgccgca
     2641 atccgggtac ggtgccgccg acggcctccc gactcgcgga agtttcgacc cgcctgcagg
     2701 cgatgcgcca caactggctg gcgctggcga ccgagttcga tgagctcgca gcacgcgggt
     2761 ccgacggcat gaaggagctt cttaccatcg gttgggcgct caagctcaac aacctcaaga
     2821 tcggcgcctc gcaggccgcc ccgcagatcg tccacgaggc gctgcagatc accggtatcc
     2881 tcggctacaa gaacgactcg cctttcagcc tggggcgcca ctaccgggac gtgctgtccg
     2941 cttccctcat gatttccaac gaccgcatcc tcggcaagaa cgcctcgatg ctgctcatct
     3001 acaaggatgt gtgaggtgca agtgcccgcg cagcccggca cacaacgcaa catgactggc
     3061 ccgtgaacta cgcggacgat cgccgatctt ccgagaacaa agtcatttcc
//
LOCUS       DATLKL010000118         1730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_114049, whole genome shotgun sequence.
ACCESSION   DATLKL010000118 DATLKL010000000
VERSION     DATLKL010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114049"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VM713_01555"
     CDS             <1..1175
                     /locus_tag="VM713_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVH82977.1"
                     /translation="RQAFAITGLSIRSIPARWAPSLVIVIGLAGVVAVFTALLAMAAG
                     FESTLKATGSRDNALILRGGSDAELNSAFDRDSTDLIKQEPGIRPGADGKPLASAELM
                     VIAELVRKDDVKSGANITMRGVEPEGFALRPQLKIIAGRNFTPGLRELIVGRGVLAQF
                     QGANIGQTLRMRGSEWQVVGEFASGDSHDSEIWTDINVARSTFGRLGSSSVLAALDGP
                     DGFERLKAAIAAEPRLSMDVLREDDYFSGQTKQFRATIGVLAGVVTVIMGLGAVFAAL
                     NSMYAAVTARGKEIATLRALGFGGFPVVVSVMIEALLLALGGALLGALIAYLLFNNLS
                     VSTLGQNFTQVVFSFKVTPELVARGLTVAVLIGMLGGFLPALRAARVPVTASLRES"
     gene            complement(1192..>1730)
                     /locus_tag="VM713_01560"
     CDS             complement(1192..>1730)
                     /locus_tag="VM713_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HVH82978.1"
                     /translation="HAQGLKVHMDGARFANAVAFLGCQPADVTWRAGVDVLSFGATKN
                     GALTAEAVVFFDLTLVRDFELRRKRAGHLLSKSRYVAAQLLAYVESGIWLRNAERTNR
                     LAQRIGAAAGTALLHPVEANEVFVKLGAARRQALRAAGFEFYDWGAEAEGDARFVVSW
                     DQPEQHVHALCEALTRVK"
BASE COUNT          255 a          595 c          605 g          275 t
ORIGIN      
        1 tgagacaggc attcgcgatc accggcctca gcatccgcag catccccgcg cgctgggcgc
       61 catcgctcgt catcgtcatc gggctcgcgg gcgtggtggc ggtgttcacc gcgctgctcg
      121 ccatggccgc aggcttcgag agcacgctca aggccacggg cagccgcgat aacgccctca
      181 ttctgcgcgg cgggtccgac gccgagctca actccgcctt cgaccgcgac tcgaccgacc
      241 tcatcaagca ggagccgggc atccgccccg gggcggacgg caagcccctc gcctccgccg
      301 agctcatggt gatcgccgag ctggtgcgca aggatgacgt caagagcggc gcgaacatca
      361 ccatgcgggg cgtggagccg gagggcttcg cgctgcgccc gcagctcaag atcatcgccg
      421 gccgcaactt cacccccggg ctgcgcgagc tgatcgtcgg ccgcggcgtg ctcgcgcagt
      481 ttcagggcgc gaacatcggc cagaccctgc gcatgcgcgg ctccgaatgg caggtggtgg
      541 gggagttcgc ctcgggagac tctcatgaca gtgagatctg gaccgacatc aacgtcgcgc
      601 gctcgacctt cgggcgcctc ggttcgagct cggtgctcgc ggcgctcgac gggcccgacg
      661 gcttcgagcg gctcaaggcc gccattgcgg ccgagccgcg cctgtcgatg gacgtgctgc
      721 gcgaggacga ttatttcagc ggtcagacca agcagttccg tgcgaccatc ggggtgctcg
      781 ccggagtggt gactgtcatc atggggctgg gcgcagtgtt cgccgcgctc aacagcatgt
      841 atgccgcggt caccgcgcgc ggcaaggaga tcgcgacgct ccgggcgctc ggcttcgggg
      901 gcttcccggt cgtggtgtcg gtcatgatcg aggcgctgct gctcgcgctc ggcggggcct
      961 tgctcggtgc gctgatcgcc tatctgctct tcaacaacct gtcggtgtcg accctgggac
     1021 agaacttcac ccaggtggtg ttcagcttca aggtgacgcc ggagctggtc gcccgcgggc
     1081 tcaccgtcgc ggtcctcatc ggtatgctgg gagggttcct gccggcgctg cgggcggcgc
     1141 gggtgccggt gacggcgagc ctgcgcgaga gctgaggacg gcgcgaactg ctcacttcac
     1201 gcgcgtgagc gcctcgcaca gcgcgtgcac gtgctgctcg ggttggtccc acgagactac
     1261 gaagcgcgcg tctccctcgg cctccgcgcc ccagtcatag aactcgaacc ccgcggcgcg
     1321 cagcgcctgc cggcgcgcgg cgccgagctt cacgaacacc tcgttcgcct cgaccgggtg
     1381 cagcagggcc gtgccggccg ccgcaccgat gcgctgcgcc aggcggttgg tgcgctcggc
     1441 gttacgcagc cagatccccg attcgacata ggcgagcagc tgtgcagcga cgtagcgcga
     1501 tttcgacagc agatgaccgg cgcgcttgcg gcgcagctcg aagtcgcgca ccagcgtcag
     1561 gtcgaagaac accacggcct cggcggtcag cgccccgttc ttggtggccc cgaaggacag
     1621 aacgtcgacc ccggcgcgcc aggtgacatc agcgggttgg cagccgagaa aggcgacggc
     1681 gttggcgaag cgtgcgccgt ccatatgcac cttcagacct tgggcgtgcg
//
LOCUS       DATLKL010000119         5317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_12501, whole genome shotgun sequence.
ACCESSION   DATLKL010000119 DATLKL010000000
VERSION     DATLKL010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12501"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VM713_01565"
     CDS             <1..787
                     /locus_tag="VM713_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVH82979.1"
                     /translation="GDLTLARTESVTWKGPGGRTLDGVLTYPASYREGRRYPLVLLVH
                     GGPTSSSRARFGLLRQVMASHEWLVFEPNYRGSDNSGNAFAAAIYRDAGEGPGEDVMS
                     GVEYLKRCGLVDPARMAVSGWSYGGFMTAWLAGHYPVWKAAVAGAAVTDWVQMYDLSD
                     GNVTQSEATGASPYVGDGMAVNRRQSPSSAMTKIRAPTLIMCNTGDFRVPITQSYGLY
                     RALKDNHVTTEFYAIPSGGHFPGDPVRQIDVYQRWVDWLPRFL"
     gene            805..1491
                     /locus_tag="VM713_01570"
     CDS             805..1491
                     /locus_tag="VM713_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HVH82980.1"
                     /translation="MRDTLAGVRACVFDAYGTLFDVGSAVASLSDSSLAQRGAALSSL
                     WREKQLQYTWLRAVQGRHVDFWQVTGDALDFALETLGIDSPQLRERLMKLYLTLAAYP
                     EVPQMLERLKRAAFGIAILSNGSPQMLRAAVAAAGIGALVDAVLSVEEVGVYKPHPKV
                     YQLAVDRLALAPGSICFVSANAWDAWAAAEFGMRAVWCNRQRQPPERLPGQPEQAISS
                     LAELPALLGA"
     gene            complement(1519..2160)
                     /locus_tag="VM713_01575"
     CDS             complement(1519..2160)
                     /locus_tag="VM713_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82981.1"
                     /translation="MKRLLLLIAVASLAIPACAPPGARNTGVYLLIDTSGSYNRQVTK
                     AEQITLFALTRLQASDSIAVARIDTGSFTEKNIIAKATFDDRPSTANAQKRAFASRVE
                     KFIDQSPPAAYTDITGGLLQAIEFLNEKRTSRKEILILSDMKEELAKGYVRDIPLDFK
                     GFDVVALNVSKLRSDNFDPRLYLARLDQWRSRVEKGGGHWRVINDLDHLDGLL"
     gene            complement(2157..3602)
                     /locus_tag="VM713_01580"
     CDS             complement(2157..3602)
                     /locus_tag="VM713_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82982.1"
                     /translation="MTLSNLLTITPWPAVSAAILLLLLMAALYFARGTAHQLILAAAG
                     ALSRGLRVASHAVIRGQERLAARNREVLLAAGREAKERIIEREFSRVADTVRKDLAGY
                     PDMHRRLSESIQRIEEDQEEAAEVPPEAPGWAQAVKVVADLDKRNPATEILADIHKSL
                     VKAHDEAMADYRKASGERHALLRRMMPDWRVIRETLGHVGKSVESVIGRSQTIDRHMH
                     EYEAIVKGEDRAVSVLSSSSIVYFFISLMVLAVATAGTAVNFTLIARPMAEMVGGTAF
                     IGGYRTADIAALVIIMVEISMGLFLMESLRITRLFPVISALSDKIRVRMVIITFLILL
                     LMASIEAGLAYMREVLLLDEMATNALLRGEGSGTLVNSHLWITTAAQMGMGFVLPFAL
                     VFVAIPLETFVHSLRSVVGLICTAVLQSVALVLRLLGNGFRHLGTILCRVYDLPLFVP
                     LWIETRILTGNGHAAAPAKDAERKVWKGAQS"
     regulatory      complement(3714..3797)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4033..4656
                     /locus_tag="VM713_01585"
     CDS             4033..4656
                     /locus_tag="VM713_01585"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HVH82983.1"
                     /translation="MFTSLIYEARIWPVLWRRRLAYYFGFGAKEHLGFAGLLVLLIIV
                     AAGGIYFACVDGTSLEPGRIEVLQHEEARAQRRADELQCLAENVYFEARGEPLTGQYA
                     VAEVTLNRTRAQHFPHTICAVVHEARWDPIRRRHVADFSWTELGALSPEDGPAWKQAL
                     AVASAVYDDLHPAVVPEALFYHATSVRPDWARARRPVATIGNHIFYR"
     gene            complement(4687..5241)
                     /locus_tag="VM713_01590"
     CDS             complement(4687..5241)
                     /locus_tag="VM713_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82984.1"
                     /translation="MHAAPAPTDYPSTHELARRALLAFIVTFIAARITVLLIMAQRIP
                     NLYFFLSGTHVHHLNYGIFLLAAVGAYTLLATPSARAARRAAFVYGIAMALTFDEFGM
                     WLHLGGSYWQRASLDATVVVAALLAFLGYASSIEKFEARHKRAFMVLGVALLAFAAAL
                     YYASVYLGNVAGPRLRELELASSP"
BASE COUNT          882 a         1725 c         1816 g          894 t
ORIGIN      
        1 gggcgatctc acgctggcgc gcaccgagag tgtcacctgg aagggaccgg gcggcagaac
       61 gctcgacggc gtcctcacct atcccgcgag ctatcgcgag ggccggcgtt acccgctggt
      121 gcttcttgtt catggcggcc cgacttcctc ctcgcgcgcg cgcttcggcc tcctgcggca
      181 ggtgatggcg agccacgagt ggctggtctt cgagcccaac taccgcggca gcgacaacag
      241 cggcaatgcc tttgctgcag cgatttaccg cgatgcgggt gaggggccgg gtgaggacgt
      301 gatgtcgggc gttgagtatc tcaagcgctg cggcctagtc gatccggcgc gcatggcagt
      361 gagcggctgg tcttacggcg ggttcatgac cgcctggctc gccgggcatt acccggtgtg
      421 gaaggccgcc gtcgcgggcg ccgccgtcac cgactgggtg cagatgtacg atctgtccga
      481 cggcaacgtc acgcaatccg aagccaccgg ggcctccccc tatgtgggcg acggcatggc
      541 ggtcaatcgc cgccagtcgc cctcgagcgc gatgacgaag atcagggcac cgaccctcat
      601 catgtgcaac accggggact ttcgcgttcc catcacgcag tcatacgggt tataccgggc
      661 gctcaaggac aaccacgtca cgaccgagtt ttacgcgatt ccgagcggag gacactttcc
      721 cggcgatccg gtgcgtcaga tcgacgtcta tcagcgctgg gtcgactggc tgccgcgatt
      781 cctgtagagc gttggagtct ggtcgtgagg gacaccctgg cgggcgtacg cgcctgcgta
      841 ttcgatgctt acggcacgtt gttcgatgtc ggctcggcgg tcgcgtcctt gagcgattcc
      901 tccctcgcgc aacggggcgc ggccctgagc tccctgtggc gggagaagca actgcagtac
      961 acctggctgc gcgccgtgca gggacggcac gtcgatttct ggcaggtgac gggcgacgcg
     1021 ctcgacttcg ctctcgagac actcgggatc gacagcccgc agctgcgcga gcggctcatg
     1081 aagctctacc tgacgctcgc tgcgtacccc gaggttccgc agatgctcga gcgactgaag
     1141 agggcggcgt ttgggatcgc cattctctcg aacggctcgc cgcagatgct gcgtgcggca
     1201 gtggccgcgg cgggcatcgg cgcgctggtc gatgcggttt tgtcggtcga agaggtcggc
     1261 gtctacaagc cgcacccgaa ggtctatcag ctcgctgtcg accggctggc actcgctccc
     1321 ggctccatct gctttgtgtc cgcgaacgcc tgggatgcgt gggcggcggc ggagttcggg
     1381 atgcgcgcgg tgtggtgcaa ccggcagcgg caaccacccg agcggctgcc ggggcagccg
     1441 gagcaggcga tcagctcgct ggcggagctg ccggcgctgc tcggagcgta gcgggccgat
     1501 tgcccggcgc cagggcgcct acagcagtcc gtccaggtga tcgaggtcgt tgatgacgcg
     1561 ccagtgaccg ccgcccttct cgacgcggct acgccactga tcgaggcgcg cgaggtagag
     1621 gcgcgggtcg aagttgtcgc tgcgcagctt gctcacgttg agcgcgacga catcgaagcc
     1681 cttgaagtcg agcggaatgt cgcgcacgta gcccttggcg agctcctcct tcatgtccga
     1741 gaggatcagg atctccttgc gcgacgtgcg cttctcgttc aggaactcga tcgcctgcag
     1801 cagtccgccg gtgatgtccg tataggccgc gggtggcgac tgatcgatga acttctccac
     1861 gcggctggcg aacgcgcgct tctgcgcgtt ggccgtgctc gggcgatcat cgaaggtcgc
     1921 cttggcgatg atgttcttct cggtgaagct gccggtgtcg atgcgcgcga ccgcgatcga
     1981 gtccgacgcc tgcagccgcg tcagcgcaaa gagggtgatc tgctccgcct tggtgacctg
     2041 ccggttgtag gaaccggacg tgtcgatcag caggtagacc cccgtgttgc gggcgccggg
     2101 cggcgcgcag gcggggatgg ccagcgatgc gacggcgatc agcagcagca agcgcttcat
     2161 gattgcgccc ccttccatac cttgcgctcg gcgtccttgg ccggcgcggc ggcatgaccg
     2221 ttaccggtca gtatccgggt ctcgatccac agcggcacga acagcggcag gtcgtagacc
     2281 cggcagagga tcgtgccgag gtggcggaag ccgttgccca gcagccgcag caccagagcc
     2341 accgactgca gcaccgcggt gcagatgagg cccaccacgc tgcgcaggga atgcacgaag
     2401 gtctcgagcg ggatcgctac gaagaccagc gcgaacggca ggacgaagcc catgcccatc
     2461 tgcgccgcgg tggtgatcca caggtgggaa ttcaccagcg taccggaacc ctcgccgcgc
     2521 agcagcgcgt tggtcgccat ctcgtccagc agcagcacct cgcgcatata ggcgagcccg
     2581 gcttcgatgc tcgccatgag cagcaggatg aggaaggtga tgatgaccat ccgcacccga
     2641 atcttgtccg acagggcgct gatcaccggg aacagccgcg tgatgcgcag cgactccatg
     2701 aggaacagcc ccatggagat ctccaccatg atgatgacca gtgcggcgat gtcggcggtg
     2761 cggtagccgc cgatgaaggc cgtgccgccg accatctcgg ccatgggacg cgcgatcagg
     2821 gtgaaattga ccgccgtacc ggcggtcgcc accgccagca ccatcagcga gatgaagaag
     2881 tacacgatcg acgaggagga gagcaccgac acggcgcgat cctcgccctt cacgatcgcc
     2941 tcgtactcgt gcatgtggcg gtcgatggtc tgcgaacgtc cgatgacgct ctcgacgctc
     3001 ttgcccacgt gcccgagcgt ctcgcggatc acccgccagt cgggcatcat ccgccgcagc
     3061 agcgcatggc gctcaccgct cgccttgcgg tagtcggcca tcgcctcgtc gtgggccttg
     3121 accagggatt tgtgaatgtc ggcaaggatc tcggtggcgg ggttgcgctt gtcgaggtcc
     3181 gcgacgacct tgaccgcctg tgcccagccg ggtgcttcgg gcgggacttc cgcggcctcc
     3241 tcctggtcct cctcgatgcg ctgaatcgac tcgctgaggc gccggtgcat gtccggatag
     3301 cccgccagat ccttgcgcac cgtgtcggcg acgcgggaaa attcacgctc gatgatgcgc
     3361 tccttcgcct cgcggcccgc cgcgagcaac acctcccgat tgcgcgccgc gagccgctcc
     3421 tgtccgcgaa tgaccgcgtg cgaagcgaca cgcaaccccc gcgagagcgc gccggctgcc
     3481 gcgaggatga gctggtgcgc cgtgcccctg gcgaagtaca gggcagccat cagcagcagc
     3541 agcagtatgg cagccgacac cgcgggccac ggagtgatcg tcagcaggtt ggacagagtc
     3601 attggtgtgc tcgcttagcg gttgtgcaaa tggggtgggg cacgccccgg cggcagcgcg
     3661 accccgagta gtgaacggcg gatgacctac agcgccgaga cctggcgttc catgctcgca
     3721 accccgctgt catgggaaag gtcccttaga ccggtggctt tgcggccctt ctcccgaagg
     3781 gtctgccctc gcagatgaac ctgaacttaa tgtaagcccc ggaccgcggc ggcgtcgtgc
     3841 gctgagtcac agtgcgctga gtgacggcgc gcggccttgc ttgaggccct caatcgggcc
     3901 ccgagcgtcc cggtgcgaca taataccccg cttggactca gcgatgcgga cacgcgcacg
     3961 ctcccgccgc cgccgccgcg tgcacactac ggcttaagga atagcgtcca gggatgccgc
     4021 acccacctat atatgttcac ctcactaatc tacgaggctc gcatctggcc ggtactctgg
     4081 cggcgccggc ttgcctacta cttcggcttc ggcgcgaagg agcatctcgg cttcgcaggt
     4141 ctgctcgtgt tgctcataat cgtggccgcg ggcggcatct actttgcgtg tgtggacggc
     4201 acgagcctcg agcctgggcg gatcgaggtt ctgcagcacg aagaggcgcg cgcgcagcgg
     4261 cgcgcggatg agctgcaatg cctcgccgag aatgtctact tcgaggctcg cggtgagccg
     4321 ctcaccggcc agtacgcggt ggccgaggtc accctgaacc gcacgcgcgc gcagcacttc
     4381 ccgcacacga tctgcgccgt ggtgcacgag gcgcgttggg atccgatccg ccggcgccac
     4441 gtcgcggact tctcctggac cgagctcggc gcactgtctc cggaggacgg acccgcctgg
     4501 aaacaggcgc tggccgtggc gagtgcggtc tacgacgatc tgcatccggc ggtcgtgccg
     4561 gaagcgctct tttatcacgc gaccagcgtg cggccggatt gggcgcgcgc ccggcgcccg
     4621 gtcgccacga tcggcaatca cattttctat cgctgagccg cgagcgcgcg ccgtctcgag
     4681 gcgcgctcag ggcgaggaag cgagctcgag ctcccgcagg cgcgggccgg ccacgttgcc
     4741 gaggtacacg gacgcgtagt agagggcggc ggcgaatgcc agcaacgcca ccccgaggac
     4801 catgaacgct ctcttgtggc gcgcctcgaa tttctcgatc gagcttgcgt aacccagaaa
     4861 ggcgagcagc gcggcgacga ccaccgtcgc atcgaggctg gcacgctgcc agtagctgcc
     4921 gccgagatgc aaccacatgc cgaactcatc gaaagtgagt gccatggcga tcccgtaaac
     4981 gaatgcggcg cggcgcgccg cgcgcgcact cggcgtggcc agcagcgtgt aagcgccgac
     5041 cgccgccagg aggaaaatcc cgtagttgag atggtggacg tgggtgccac tcaggaagaa
     5101 atagaggttg gggatgcgct gcgccatgat caacagcacc gtgatgcgcg cggcgatgaa
     5161 ggtcacgatg aatgcgagca gcgcgcgtcg cgccagctcg tgcgtgctgg ggtaatcggt
     5221 cggtgcggga gccgcgtgca ttcggggcgg attctaacag cgcgggtaac agcggctccg
     5281 gcgggggcca tcattcgagt atttgatcgc agccggc
//
LOCUS       DATLKL010000120         1758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_91547, whole genome shotgun sequence.
ACCESSION   DATLKL010000120 DATLKL010000000
VERSION     DATLKL010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91547"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VM713_01595"
     CDS             complement(<1..312)
                     /locus_tag="VM713_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HVH82985.1"
                     /translation="MKFFLNTASLKDISWAAQAGLIDGVTTNPSLLAKQAGDLDPRDV
                     LKEICSLVEGPVSAEVVAVDADAMVREGIDLAKIADNIVVKIPMLEAGMVAVRRLATE
                     GI"
     gene            complement(309..1100)
                     /gene="truA"
                     /locus_tag="VM713_01600"
     CDS             complement(309..1100)
                     /gene="truA"
                     /locus_tag="VM713_01600"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HVH82986.1"
                     /translation="MTSRPTLAVVHYDGAQFVGWQRQRAGRSVQGEFESALERLLGHH
                     TPAIGAGRTDSGVHALGQGVSFLASERWTSDLDGLARALNALLPADVWVERVRVMCPG
                     FDARKSAVARRYRYVIGTDAAARSPFRRPYEWALGHPLDAARLAQAAESVVGEHDFRG
                     LAATSGGAAKPHYRSRVALAEWAPRTDGAGVTFTIEADRFLHRMVRFLVGAMVDVALG
                     RRPLEDLIRLLAATDNQAASPPAPPQGLYLVAVRYPAQLYAGSEA"
     gene            1338..>1758
                     /gene="trpD"
                     /locus_tag="VM713_01605"
     CDS             1338..>1758
                     /gene="trpD"
                     /locus_tag="VM713_01605"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Catalyzes the conversion of
                     N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to
                     anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HVH82987.1"
                     /translation="MFGAVMRGEATPAQLGALLMGLRVKGETAEEVAGAARALREAMV
                     KVAASGAHLVDTCGTGGGQVSTFNISTAAAFLAAGAGASVAKHGNRSFTSRCGSADVL
                     EALGLRVALEASEAARVLREACITFLFAPQFHPAMRHA"
BASE COUNT          256 a          619 c          624 g          259 t
ORIGIN      
        1 gatgccctcc gtggcgagcc gccgcacggc gaccatgccc gcctcgagca tcgggatctt
       61 gacgacgatg ttgtcggcga tcttcgcgag gtcgatcccc tcgcgcacca tggcgtcggc
      121 gtcgacggcc accacttccg ccgacacggg gccctcgacg agcgagcaga tctccttcag
      181 cacgtcccgc gggtccaggt cacctgcctg tttcgcgagc agcgacgggt tcgtggtgac
      241 cccgtcgatc aggccggcct gggccgccca gctgatgtcc ttgagactgg cggtgttgag
      301 gaagaacttc atgcctcact cccggcgtag agttgagcgg ggtagcgcac cgccacgagg
      361 tagagcccct gcggcggggc gggcgggctg gccgcctggt tatcggtcgc ggcgaggaga
      421 cggatcagat cctcgagggg gcgccggcct agtgcgacat cgaccatcgc gcccacgagg
      481 aagcgcacca tacgatgcag gaaccggtcg gcttcgatcg tgaaggtcac ccccgctccg
      541 tcggtccgcg gcgcccactc ggcgagcgcg acgcggctgc ggtaatgggg cttcgcggcg
      601 ccgccactcg tggcggcgag gccgcggaag tcgtgctccc cgacgacgga ctcagcggcc
      661 tgcgcgagcc gtgcggcatc gagcgggtgc ccgagcgccc attcgtacgg ccggcggaac
      721 ggcgagcgcg cggcggcatc ggtgccgatg acgtagcggt agcgccgcgc gacggcactc
      781 ttgcgcgcat cgaagcctgg gcacatgacc cggacccgct ccacccacac gtcggccggg
      841 agcagcgcat tcaaggcgcg ggcgagcccg tcgagatcgc tcgtccagcg ctcgctcgcg
      901 aggaagctca ctccctggcc gagcgcgtgt acacccgagt cggtgcggcc ggcgccgatg
      961 gccggggtgt ggtgaccgag caggcgttcg agcgcgctct cgaactcccc ctgcacgctc
     1021 cgcccggccc gctgccgctg ccagcccacg aactgcgccc cgtcgtaatg caccacggcg
     1081 agggtgggcc ggctcgtcac gggcgaaaga tagggcgaag cggaaggtca atcaagacgc
     1141 gcaacccgtt gtggcatttg acaaaaggcg atttttccgg gtagctttgc gaggcctcaa
     1201 aatacgccct acgcttcgtg cccccgccct ccccgtcttc tcccccgcga gcgcccgagc
     1261 ccgctccgac cgcgattcag caggcgatcg cccgacttgc caagcgcgaa tcgctgtccg
     1321 agcgcgaggc gggcgacgtg tttggcgccg tcatgcgggg cgaggcaacc ccggcgcagc
     1381 tcggcgcgct gttgatgggc ctgcgcgtga agggggagac ggccgaggag gtcgcgggcg
     1441 cggcgcgcgc gctgcgcgaa gcgatggtga aggtcgcggc gagcggggcg cacctcgtcg
     1501 acacgtgtgg cactggcggt ggtcaggtct ccaccttcaa catctcgacc gcggccgcat
     1561 tcctcgcggc gggggccggt gcctcggtcg ccaagcacgg caatcgctcc ttcacgtcac
     1621 ggtgcggctc ggccgacgtc ctcgaggcgt tgggcctgcg cgtcgcgctc gaggcaagcg
     1681 aagcggcccg cgtactgcgc gaggcgtgca tcacgttcct cttcgcgccc cagtttcatc
     1741 ccgcgatgcg ccatgcag
//
LOCUS       DATLKL010000121         1733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_192972, whole genome shotgun sequence.
ACCESSION   DATLKL010000121 DATLKL010000000
VERSION     DATLKL010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192972"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /gene="asd"
                     /locus_tag="VM713_01610"
     CDS             complement(<1..833)
                     /gene="asd"
                     /locus_tag="VM713_01610"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HVH82988.1"
                     /translation="MTVRSAIARLTQQERLNFLLTNRLPRRWLTLFMGWLSRLEQPWL
                     CRVGIGIWGLFTELDLSDARETRFGSLQEFFTRQLKEGARRIDPDPEILVSPCDGIVG
                     AMGQVREGSVLQAKGFPYTLMDLLGDQELVSNYLNGSFVTLRLTSAMYHRFHAPYDCR
                     VERVTYVSGDTWNVNPIALRRVENLCCNNERAIIRCRLRASDYLITLVPVAAILVASI
                     RVHCADVLLHLKYRGPNTIPCDAQLRKGGEMGWFQHGSTMIAFAPPGIVLSPDLGQGS
                     V"
     gene            911..>1733
                     /locus_tag="VM713_01615"
     CDS             911..>1733
                     /locus_tag="VM713_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH82989.1"
                     /translation="MLLWYIAGALITLLVLRSLRLRLRLSRAKHPSLAGHARLARLLT
                     RLIRYYEYDETRFFSADEAPEEVAGARRAGFMQLAQLYAQRFAKSTALTREVARSVSD
                     LQFTARYRVPFQFGRVVRQYLPGSAFLESSAGVTVADIDGNRFFDVSGSYGVNLFGYD
                     FYKECIERGAERVRALGPVLGAYHPLVADNVRRLREISGLDEVSFHMSGTEAVMQAVR
                     LARYHARRRYLVRFCGAYHGWWGDVQPGLGNPLPPDATYTLKDMDAASLRVLRTRS"
BASE COUNT          293 a          564 c          575 g          301 t
ORIGIN      
        1 atcacgctgc cctggccaag gtcgggagaa agcacgatgc cgggtggcgc gaaggcgatc
       61 atggtggagc cgtgctggaa ccagcccatc tctccgcctt tgcgtagctg cgcatcgcag
      121 ggaatggtgt tggggccgcg gtacttcaaa tgcagcagca cgtccgcgca atgcacccgg
      181 atgctcgcca ccagaatggc ggcgaccggc acgagcgtga tgaggtagtc cgacgccctc
      241 agccggcagc gaatgatggc gcgctcgttg ttgcagcaga ggttctcgac acgccgcagc
      301 gcaatcggat tgacattcca ggtgtctccg gagacatagg tcacccgctc cacgcgacag
      361 tcatagggcg catggaagcg gtgatacata gcggaggtga ggcgcagcgt cacgaaggag
      421 ccgttgagat agttactcac cagctcctga tcgccgagca ggtccatgag cgtataggga
      481 aagcccttgg cctgaagcac cgatccttct ctcacctgac ccatggcgcc cacgatgccg
      541 tcgcagggac tgacgaggat ctcgggatcc gggtcgatgc ggcgcgcccc ttccttcagc
      601 tgccgggtga agaactcctg gagactccca aagcgggttt cccgggcatc gctcagatcg
      661 agctcggtaa agaggcccca gatcccaatg cccacacggc agagccaggg ttgctcgagg
      721 cggctcagcc atcccatgaa cagggtgagc cagcgacggg gcaggcggtt ggtcaaaagg
      781 aaattcagcc gctcctgctg cgtgagccgg gcgatagccg agcggactgt catctggctg
      841 tcacgggact gaaatagttt gccgccgggg cgatcggccc cgggttggct gcccttgggg
      901 ctgaagaatc atgctgctgt ggtacatcgc cggcgctctg atcacgctgc tcgtgctgcg
      961 cagcctgcgg ctgcggctgc ggctctcgcg cgccaagcac ccctcgctcg ctggacacgc
     1021 gcgcctcgcg cggctcctga cacgcctcat tcgctactac gaatacgacg agacgcgctt
     1081 cttcagcgcg gacgaggcac ccgaggaagt cgccggcgcg cgccgtgcgg gtttcatgca
     1141 gctcgcgcag ctctacgcgc agcgcttcgc gaagagcact gcgctcaccc gcgaggtggc
     1201 gcgctcggtg tccgacctgc agttcacggc gcgctaccgg gtgccctttc agttcggccg
     1261 cgtggtccga cagtacctcc cgggtagcgc attcctcgag tcctctgcgg gtgtcaccgt
     1321 ggccgacatt gacggcaacc gcttcttcga cgtgagcggc tcgtacggcg tgaacctctt
     1381 cggctacgat ttttacaagg agtgcatcga gcgcggcgcc gaacgggtgc gcgcgctcgg
     1441 accggtgctc ggcgcctacc atccgctcgt ggccgataac gtgcgccgcc tgcgcgagat
     1501 ctcgggactc gacgaggtct cgttccacat gtcgggtacc gaggccgtca tgcaggccgt
     1561 gcgcctggcg cgctaccacg cgcgccgccg ttacctggtg cgcttctgcg gtgcgtatca
     1621 cggctggtgg ggagatgtgc agccgggcct cggcaacccg ctgccccccg atgccaccta
     1681 cacgctgaag gacatggatg cggcatcgtt gcgggtgctg cgcacgcgca gcg
//
LOCUS       DATLKL010000122         1552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_181813, whole genome shotgun sequence.
ACCESSION   DATLKL010000122 DATLKL010000000
VERSION     DATLKL010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181813"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VM713_01620"
     CDS             <1..506
                     /locus_tag="VM713_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVH82990.1"
                     /translation="GFYVSDWTQNHANFFRSIEITKSMMFVILLMLVAVAAFNIVATL
                     VMIVKEKQTDIAILRTLGAAPRNVLATFAVQGVLIGLTGTLLGAGLGTLVSDNLEALV
                     AGLERLLGVQFLDASVYYLSDLPAYVERLDVLKVCGVALLLCALATVYPAWRAARTAP
                     AEALRHE"
     gene            499..1188
                     /gene="lolD"
                     /locus_tag="VM713_01625"
     CDS             499..1188
                     /gene="lolD"
                     /locus_tag="VM713_01625"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HVH82991.1"
                     /translation="MSEAVLEAREVHKSFRQGPVVLPVLTGVQLTVAAGERVAIVGAS
                     GSGKTTLLQILGGLDAPTSGAVLIAGRDIHALEESERGVLRNRALGFVYQFHHLLPEF
                     SALENVAMPLLVRRTPVPEARAAARRLLERVGLGERLTHRPHQLSGGERQRAAVARAL
                     VTQPSLVLADEPTGNLDGGNAESVFSLMLELNRERATSLVVVTHDLRLARRMERVYEL
                     EGGTLSAGFKQ"
     gene            complement(1178..>1552)
                     /locus_tag="VM713_01630"
     CDS             complement(1178..>1552)
                     /locus_tag="VM713_01630"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HVH82992.1"
                     /translation="SFVPLPIHLLLAVIVALVWRINLPVIYGTTWVVANPFSLVPLYY
                     FAYRVGVAVLHVPRQHFRFVPDWRWFQHALAPVWQPFAVGCLVCAAVSGVLGWLVLEL
                     VWRWNVASRYRTRHAAPAAPTV"
BASE COUNT          249 a          516 c          555 g          232 t
ORIGIN      
        1 ggggctttta cgtgagcgac tggacgcaga accatgcgaa cttcttccgt tcgatcgaga
       61 tcacgaagtc gatgatgttc gtgatcctgc tgatgctggt cgccgtcgcc gccttcaaca
      121 tcgtcgcgac gctggtgatg atcgtgaagg agaagcagac ggacatcgcc atcctgcgca
      181 ccctcggggc ggcgccgcgc aacgtgctcg ccaccttcgc cgtgcagggg gtgctgatcg
      241 ggctcaccgg tacgctgctc ggcgcgggac tcggcacgct ggtgtccgac aacctcgagg
      301 cactggtcgc ggggctcgag cggctgctcg gggtgcagtt tctcgacgcc agcgtgtact
      361 acctgagcga tctgccggcg tacgtcgagc gactcgacgt actcaaggtg tgcggcgtgg
      421 cgctgctgct gtgcgcgctc gcgaccgttt atccggcgtg gcgcgcggcg cgcaccgcgc
      481 ccgcggaagc gctgcgccat gagtgaagcc gtcctcgagg cgcgcgaggt acacaagagc
      541 ttccgccagg ggccggtggt gctgcccgtg ctcacagggg tgcagctgac agtggcggcg
      601 ggcgagcgcg tagcgatcgt cggcgcttcc gggtcgggca agacgacgtt gctgcagatc
      661 ctcggcgggc tcgacgcgcc gaccagcggc gcggtgctga tcgcaggccg cgacatccat
      721 gcgctcgagg agagtgagcg cggcgtgctg cgcaaccgcg cgctcggctt cgtctaccag
      781 ttccatcatc tgctgccgga gttctcggcg ctggagaacg tggcgatgcc gctgctggtg
      841 cggcgcacgc ccgtgcccga ggcgcgcgcc gcagcgcgcc gtctcctcga gcgggtgggc
      901 ctcggcgagc gactcaccca tcgcccccac cagctctcgg gcggagagcg gcaacgcgcg
      961 gcggtggcgc gcgccctggt gacgcagccg tcgctcgtgc tggcggacga gccgaccggg
     1021 aacctcgacg gcggcaacgc cgagagcgtc ttttccctga tgctggagct caaccgcgaa
     1081 cgcgctacca gcctcgtagt ggtgacgcat gacctccggc tcgcgcgccg catggagcgc
     1141 gtctacgagc tcgagggagg aacgctcagc gctggcttca aacagtaggt gccgccggag
     1201 ccgcgtgccg cgtccggtag cggctcgcca cgttccagcg ccagacgagc tcgagtacga
     1261 gccagccgag cacgccgctc accgccgcgc agaccaggca gccgacggcg aacggctgcc
     1321 acaccggcgc aagtgcgtgc tggaaccagc gccagtcggg gacgaacctg aaatgctggc
     1381 gcggcacgtg caggacagca acgcccaccc ggtaggcgaa gtaatagagt ggcaccaggc
     1441 tgaatggatt cgcgaccacc caggtcgtgc cgtagatcac cgggaggttg atccgccaga
     1501 cgagcgccac gatcaccgcg agcagcaggt ggatcggcag cggtacgaat ga
//
LOCUS       DATLKL010000123         2221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_136715, whole genome shotgun sequence.
ACCESSION   DATLKL010000123 DATLKL010000000
VERSION     DATLKL010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136715"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..804
                     /locus_tag="VM713_01635"
     CDS             376..804
                     /locus_tag="VM713_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH82993.1"
                     /translation="MRPPAAPAIVIHSLAQARAAARAAVELGVAVELWSASGAAAYAG
                     PGWFKAVLEATRAAEPDARISAVLDCADLPGLVLGTFRIGLEAACFTGPDRVAAKLAD
                     IAAQHRARLVRRRPNHALDLRRAADPYALCRTWLAEGGRP"
     gene            907..1836
                     /locus_tag="VM713_01640"
     CDS             907..1836
                     /locus_tag="VM713_01640"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006704.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HVH82994.1"
                     /translation="MKITPRVKRILDNYESDCPGTKANLARILMSGRLAGTGHLVILP
                     VDQGFEHGPARSFAPNPAAYDPHYHYQLAIDAGLSAYAAPLGMIEAGADSFAGQIPLI
                     LKVNSANSMSRAKEGADQAVTASADDALRLGCAAVGFTIYPGSDAAYGMFEEVRAIAG
                     EAKRKGLAVVIWSYPRGGALSKAGETAIDISAYAAQIAALLGAHIIKVKPPTAHLELE
                     AAKKVYESQRIDASTLAARIRHVVQAAFNGRRLVVFSGGEAKDEAGILEEARAIREGG
                     GTGSIIGRNSFQRPREKALELLGKVIDIYKGKD"
     gene            1850..>2221
                     /locus_tag="VM713_01645"
     CDS             1850..>2221
                     /locus_tag="VM713_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HVH82995.1"
                     /translation="MSDAPSCRLYLVTPAALKPQAFARELEGALDAGDVACLQLRLKG
                     ADDDAVLRAAERFVPICARRNVAFLVNDRPDLALKAGADGVHIGREDASYGEARRLLG
                     PDKIVGVTCHASRDLAIEAAEA"
BASE COUNT          339 a          799 c          754 g          329 t
ORIGIN      
        1 aggtccgcga ccgtgctgat gtccgagcgg ttgaagccat tggcctggag ctcgtccacc
       61 gcggcctgca gatgctcctg gtcgtcgaag acaccgacca cctcccgcac caagccggca
      121 tgacgagagg ttgccgcctc ccccgatgac gtcgacatgc aagcctcctc gttcggcgct
      181 cctgcataag gagcgctttg gtcattctcc ctgtgaagtg agcgggcggc aaccccttgt
      241 caatggccca gagcggcggg ctctcgcccc gcccgggctc ggccgaggcc ggcgcgcgct
      301 ggccgaatta aaccggaggc cgcgccgtga taagtcttgg acagcatggc aaggaaaccg
      361 cctaccccca cccctttgag gccccccgcc gcgcctgcca tcgtcatcca ttcgctggcc
      421 caggcccgtg cggccgcccg cgcggccgtc gagctcggcg tcgcggtaga gctgtggagc
      481 gcgtccgggg ccgccgccta cgccggaccg ggctggttca aggcggtcct cgaggcgacc
      541 cgcgcggccg agcccgatgc gcggatttcc gctgtgctcg actgcgccga ccttcctggc
      601 ctggtcctcg gcacctttcg catcggactc gaggcggcgt gcttcacggg ccccgaccgg
      661 gttgcggcaa agctcgccga catcgcggct cagcatcgcg cccggctcgt gcgccggcgg
      721 ccaaatcacg cattggacct gcgccgcgcc gccgaccctt atgcgctgtg ccggacctgg
      781 ctcgccgagg ggggccggcc ttagggttcg ccgcgcagca gctccgcccc tccgttgcaa
      841 tggaggtgag gctcggatat tgaagggccg ccgccggcga gcaggcgctc acccggagaa
      901 ttcgccatga agatcacgcc gcgcgttaag cgcattctgg acaattacga gagcgactgc
      961 ccgggcacca aggcgaacct cgcgcgcatc ctcatgagcg ggaggctcgc cggcaccggg
     1021 cacctggtta tcctgccggt ggaccagggc ttcgagcatg gcccggcgcg cagcttcgcc
     1081 cccaacccag ccgcctacga cccccattac cactatcagc tcgcaatcga cgccgggctt
     1141 tcggcctatg ccgcgccgct cggcatgatc gaggccgggg cggacagctt cgccggccag
     1201 atcccgctga tcctcaaggt caacagtgcg aacagcatgt ctcgggcaaa ggagggggcg
     1261 gaccaggcgg tcacggcctc ggccgacgat gccctgaggc tcggctgcgc agcggtcggg
     1321 ttcaccatct atcccggctc ggacgccgcc tacggaatgt tcgaggaggt ccgcgccatc
     1381 gccggggagg ccaagcggaa gggtctcgcc gtggtgatct ggtcctatcc gcgcgggggg
     1441 gcgctgtcca aggcgggcga gactgcgatc gacatctcgg cctacgcggc gcagatcgcg
     1501 gcactcctgg gagcgcacat catcaaggtc aagccgccga ccgcacatct cgaactcgag
     1561 gcggccaaga aggtctacga gagtcagcgg atcgacgcct cgacgctcgc cgcgcgcatc
     1621 cgccacgtgg ttcaggccgc cttcaacggc cgccggctcg tggtattctc gggcggcgag
     1681 gccaaggacg aggcgggcat cctcgaggag gcgcgcgcca tccgcgaggg cggcggcacc
     1741 ggctcgatca tcggccggaa cagcttccag cggccgcgcg aaaaggcgct cgagctgctc
     1801 ggcaaagtca tcgatatcta taagggcaag gactgagcgg tatcgctcga tgagcgatgc
     1861 cccctcctgc cggctctacc tcgtcacgcc cgccgcgctc aagccgcagg ccttcgcgcg
     1921 cgagctcgag ggggcgctcg atgcgggcga tgtcgcctgc ctccagctcc gcctcaaggg
     1981 tgccgacgac gacgcggtgc tgcgcgcggc ggagcggttt gtgcccatct gcgcgcgccg
     2041 caatgtcgcc ttcctggtca acgaccgccc ggacctcgcg ctcaaggcgg gggccgacgg
     2101 cgtccatatc ggccgggagg atgcgagcta cggggaggcg cggcgcctgt tgggcccgga
     2161 caagatcgtc ggcgtcacct gccacgcctc ccgcgacctc gccatcgagg cggccgaggc
     2221 g
//
LOCUS       DATLKL010000124         2124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_114164, whole genome shotgun sequence.
ACCESSION   DATLKL010000124 DATLKL010000000
VERSION     DATLKL010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114164"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /gene="pyk"
                     /locus_tag="VM713_01650"
     CDS             <1..1198
                     /gene="pyk"
                     /locus_tag="VM713_01650"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HVH82996.1"
                     /translation="RIELAEGQAFTLDTALEPTAGTIDRVGVAYKELPRDVAAGDTLL
                     LADGQIVLDVKSVGRTAIETRVRIGGALSDRKGLNRQGGGISAPAISERDREDIRFVA
                     EEGIDYMAVSFARDAADIEQARALVRAAGGDARIVAKVERHEAVDNLAGIIDASDVVM
                     VARGDLGVEMGYAELTGLQKTIIHDTRHRNRVVIAATQMMESMIQNPVPTRAEVSDVA
                     NAVMDGTDAVMLSAESATGRYPVKAVEAMAEVIEGAEKYQLTHPRARQPSLEGVFTGT
                     EEAIAMAVMYTANHMKVRAIVALTESGATPLFMSRIRSDIPVYAFTRREATRRRVTLY
                     RGVYPVIFDVTGSASVEALYRALFARLLELKLVDLHDLVILTKGELSGVQGGTNSMQI
                     LKVVLD"
     gene            1294..>2124
                     /locus_tag="VM713_01655"
     CDS             1294..>2124
                     /locus_tag="VM713_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVH82997.1"
                     /translation="MLRASLPRLDGELRAVGLKAPLRITRDALGVPTIEAANRFDLAY
                     GTGFVHGQDRFFQMDLSRRLAAGELAELFGPVALAEDRETRLFRLRSVARAVLAAASE
                     PQRALLEAYTRGVNAGLASLGSRPWEYWVLGAPPSPWRAEDAFLVTYAMWWDLQSQDW
                     GYERLRQEVNARLRGRECGGGWKCALSFLYPERTEWDAPVGTEAPLTPAAIPPPEALN
                     IRDAHPAPAPHAATTAASAAQPDSRAGSNNWAVSGRFTATGAALVADDMHLSLRVPAI
                     W"
BASE COUNT          318 a          744 c          746 g          316 t
ORIGIN      
        1 tcgcatcgag ctcgccgaag gccaggcctt cacgctcgat accgcgctcg agccgacggc
       61 cggaaccatc gaccgggtcg gcgtcgccta caaggagctg ccgcgcgacg tcgccgccgg
      121 cgacacgctg ctgctcgccg acggtcagat cgtgctcgac gtcaagagcg tcggcaggac
      181 ggcgatcgag acgcgcgtgc gcatcggcgg cgcgctctcg gaccgcaagg ggctcaaccg
      241 gcagggcgga gggatctcgg cgccggcgat ttccgagcgc gaccgcgagg acatccgctt
      301 cgtcgccgag gagggcatcg attacatggc ggtgtccttc gcgcgcgacg ccgccgacat
      361 cgagcaggcg cgcgcactgg tgcgcgccgc gggaggcgat gcgcgaatcg tcgccaaggt
      421 ggagcgtcac gaggcggtcg ataatctcgc cggcatcatc gacgcctccg acgtcgtcat
      481 ggtggcgcgc ggcgacctcg gcgtcgagat gggctacgcc gagctcaccg ggctgcagaa
      541 gaccatcatc cacgacaccc gccaccgcaa ccgcgtggtg atcgccgcca cgcagatgat
      601 ggagtcgatg atccagaatc cggtgccgac gcgtgctgag gtgagtgacg tcgcgaacgc
      661 tgtgatggac gggaccgacg ccgtcatgct ctctgcggaa agcgccaccg gccgctatcc
      721 ggtcaaggcc gtcgaggcga tggccgaggt gatcgagggc gcggagaagt accagctcac
      781 ccacccgcgc gcccggcagc cctcgctcga gggggtgttc acgggcaccg aggaggcgat
      841 cgcgatggcc gtcatgtata cggccaacca catgaaggtg cgcgccatcg tggcgctgac
      901 ggagtcgggc gcgacgccgc tgttcatgtc gcgcatccgc tcggacattc cggtgtacgc
      961 cttcacgcgc cgcgaggcga cacgccgccg cgtgacgctc tatcggggcg tctaccctgt
     1021 gatcttcgac gtcaccggct cggcctcggt cgaagcgctc tatcgggcgc tgttcgcacg
     1081 gctgctcgag ctgaaactgg tcgacctcca cgatctggtg atcctcacca agggcgagct
     1141 gtcaggtgtg cagggtggca ccaactcgat gcagatcctg aaagtggtgc tcgactgacg
     1201 ccgcccgccc gagcccggct gcggcgcttc ctcggctacg ccggcgcggc ggtcgcgctg
     1261 ctcattctgc tcgcgtcagc cgtaacgtat gggatgctgc gcgcctccct cccgcgcctc
     1321 gacggcgagc tgcgcgcggt gggactcaag gcgccgctgc gcattacgcg cgatgcgctc
     1381 ggcgtgccga cgatcgaggc cgcgaaccgc ttcgacctcg cctacggaac tggcttcgtg
     1441 cacggtcagg accgcttctt ccagatggat ctgtcgcggc gcctcgccgc gggcgaactc
     1501 gccgagctct ttggccccgt cgccctcgct gaggaccgcg agacgcgcct cttcaggctg
     1561 cgcagcgtcg cgcgcgccgt actcgccgcg gcgagcgagc cgcagcgggc gctgctcgag
     1621 gcctacacgc gcggcgtcaa cgccgggctc gcgagcctcg ggtcgcggcc gtgggagtac
     1681 tgggtgctcg gcgcgccccc gagcccgtgg cgcgccgagg atgcctttct ggtcacttac
     1741 gccatgtggt gggacctgca gtcgcaggac tggggctacg agcggctgcg ccaggaggtg
     1801 aatgcgcgcc tccgcggacg cgagtgtggg ggcggctgga agtgcgcgct cagcttcctc
     1861 tatccggagc gcaccgaatg ggacgcgccg gtcgggacgg aagcaccact cactcccgcc
     1921 gcgattcctc cgcccgaggc tctcaatatt cgcgacgcgc acccggcgcc ggccccgcat
     1981 gccgccacga ccgcggcctc tgcagcgcag cccgacagcc gggccggcag caacaactgg
     2041 gcggtgagcg gtcgcttcac agcgaccgga gcggcgctgg ttgcagacga catgcacctg
     2101 agtctgcgcg tgccggccat atgg
//
LOCUS       DATLKL010000125         1554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_35289, whole genome shotgun sequence.
ACCESSION   DATLKL010000125 DATLKL010000000
VERSION     DATLKL010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35289"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VM713_01660"
     CDS             <1..1425
                     /locus_tag="VM713_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HVH82998.1"
                     /translation="DVFSAESEREAIRIEPFDDAIDSLTLFDPLTGAIARKIPRFTVY
                     PGAHYVTPRERLVGAVDQIREDLRVRLKELRDAGRLVEAQRLEQRTRFDMEMIKEVSY
                     CAGIENYSRYLSGRAPGEPPPCLFDYLPKNALLIVDESHQTIPQLGAMYRGDRSRKEV
                     LVEYGFRLPSALDNRPLKFEEWESLAPQMIFVSATPGDYEAKHSSQVVEQVVRPTGLV
                     DPQVEVRPVASQVDDVLSEIRKTAARGERVLITTLTKRMAEDLTEYLSEHEVRVRYLH
                     ADIETVERVEIIRDLRLGKFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSDNSL
                     IQTIGRAARNIHGRAILYADRMTGSIQRAIEETNRRRKKQLDYNVAHGITPRGIEKPV
                     TDIMEGARSEAPTPGSRRRGEKPRSAADLRPEALVKEIKRLEAEMFRRARNLEFEEAA
                     TLRDEIERLKQLELGLPAVAAAHG"
BASE COUNT          283 a          510 c          510 g          251 t
ORIGIN      
        1 gatgtcttct cggccgagtc cgagcgcgag gccatccgca tcgagccttt cgatgacgcc
       61 atcgactcgc tcacgctgtt cgatcccttg accggggcga tcgcgcgcaa gatcccgcgc
      121 ttcacggtct accccggcgc ccactacgtc accccacgcg agcggctggt cggtgcggtc
      181 gatcagatcc gcgaggacct gcgcgtgcga ctcaaggagc tgcgcgatgc cgggcggctg
      241 gtcgaggcgc agcggctcga gcagcgcacc cgcttcgaca tggagatgat caaggaggtc
      301 agctactgcg cgggcatcga gaactactcg cgctacctct cgggccgtgc gcccggggag
      361 ccgccgccgt gcctcttcga ttatctcccg aagaacgcgc tcctcatcgt cgacgagagc
      421 catcagacca tcccacagct cggcgccatg tatcgcgggg atcgctcgcg caaggaagtg
      481 ctggtcgaat acggctttcg cctgccgtcc gcgctcgata accggccgct gaagttcgag
      541 gagtgggaga gtctcgcgcc gcagatgatc tttgtgtcgg cgacccccgg cgattacgag
      601 gccaagcact cctcgcaggt ggtcgagcag gtggtgcgcc ccacgggcct cgtcgacccg
      661 caagtcgagg tgcgccccgt ggcgagccag gtggacgacg tgctctctga gatccgcaaa
      721 accgccgccc gcggcgagcg ggtcctcatc acgacgctca ccaagcgcat ggcggaggat
      781 ctcaccgagt atctctcaga gcacgaggtg cgcgtgcgct acctgcacgc cgatatcgag
      841 accgtggagc gggtcgagat catccgggat ctgcgcttag gaaagtttga cgtgctggtc
      901 gggatcaacc tgctgcgcga ggggctcgac atgcccgagg tctccctggt cgcgattctg
      961 gatgcggaca aggaaggttt cctgcgctcg gacaactcac tcatccagac aatcggccgc
     1021 gccgcgcgca acatccacgg gcgcgcgatc ctctacgcgg accggatgac gggttccata
     1081 caacgcgcca tcgaagagac gaaccggcgc cggaagaaac agctcgacta caacgtcgcc
     1141 cacggcatca cgccccgcgg gatcgagaag ccggtcaccg acatcatgga gggcgcgcgg
     1201 agcgaggcac caacgcccgg cagccgccgc cgcggcgaga agccgcgcag cgcggcggat
     1261 cttcgtcccg aggcgctggt gaaggagatc aagaggctcg aagccgagat gttccgcaga
     1321 gcacgtaacc tcgagttcga ggaggcggcg acgctgcgcg atgagatcga gaggctcaag
     1381 cagctcgagc ttgggctgcc ggccgttgcg gccgcgcacg gctagtccgc cagcgcaagc
     1441 gtaagtgctt ggaatcacac gctttagctt gcgtaccttg gcactctctt gtaatgttcg
     1501 cgcccctcgt gcgggcttca gggatttcag gcgcgtagct cagtggtaga gcat
//
LOCUS       DATLKL010000126         3410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_46458, whole genome shotgun sequence.
ACCESSION   DATLKL010000126 DATLKL010000000
VERSION     DATLKL010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46458"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VM713_01665"
     CDS             <1..487
                     /locus_tag="VM713_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVH82999.1"
                     /translation="AHPYTSSPRLAIFGASNGGLLVGACMTQRPELYGAVIADVGVMD
                     MLRFDKFGQGAGWEGDFGSPDNPQEFKALFAYSPLHNVRAGVRYPATLVITGDHDTRV
                     MPMHSFKFAAALQAAQAGQAPVLLDVELNSGHGGSQTVYRAIALHADIYTFLARNLAM
                     K"
     gene            498..2159
                     /gene="mdcA"
                     /locus_tag="VM713_01670"
     CDS             498..2159
                     /gene="mdcA"
                     /locus_tag="VM713_01670"
                     /inference="COORDINATES: protein motif:HMM:NF028267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HVH83000.1"
                     /translation="MATALEPGAGPRSWTTRPEDKAARLAGLAQYVDGKLVRRADVVA
                     VLEALLRPGDRVALEGNNQKQADFLSRALVEVDPRRVYDLHMLISSITRPEHLTLFER
                     GIARRVEFSYAGPQSLRVAQLLEDGKLEIGAIHTYIELYARMFVDLTPHVVLVCAEKA
                     DLAGNLYTGPNTEETPTLIEAAAFRHGVVLVQVNEVVDRLPRVDVPGSWVDLIVPADR
                     PFAIEPLFTRDPRQINELQILMGMMVIRGIYERHGVTALNHGIGFDTAAIELLLPTYA
                     AALGLQGRICRNWILNPHPTLIPAIESGWVESVHCFGGEVGMEDYVRARPDVFFVGGD
                     GSLRSNRVLCQLAGQYGVDLFIGSTLQMDADANSSTVTLGRLAGYGGAPNMGHDPRGR
                     RHASPAWLSLITAAGPVVRGRKLVVQLVETFSRGGVPALVESLEAVQVGRDSGFAVAP
                     VMIYGDDVSHVVSEEGIAYLYKAQGQEERRAALAAIAGATEIGRRADPRRSASLRARE
                     IIAYPEDLGVRRAEARRSLLAARSIEDLVGWSGGLYKPPARFRSW"
     gene            2164..3060
                     /locus_tag="VM713_01675"
     CDS             2164..3060
                     /locus_tag="VM713_01675"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF002315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HVH83001.1"
                     /translation="MAPAVHLRLAARPPRPAQRAAAELAERAVRALVLEALLTPKPAL
                     VDRRGCGAHRDLDLRKLLCSARALRGTFHRMALVAGTSAADVRLRERLGWLGRRGEDA
                     MLAATGGSNAHRGAIWVLGLMVAALALVGTDASAAEVAATAAGLARLPDRRAPHLPSH
                     GARVCREFGVAGARGEAHAGFPHVIEVGLPALAAARRRGAPESCARLDALLAIMSSLD
                     DTCLLHRGGWAALHAARSGARHVLALGGSATAAGAAALLALDAELLRHNASPGGCADL
                     LAACLFLDGAAHELESQHSWNA"
     gene            3048..3341
                     /locus_tag="VM713_01680"
     CDS             3048..3341
                     /locus_tag="VM713_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HVH83002.1"
                     /translation="MERLSFIYPARESVARRVHVGVVGSGDLEVLLEPSADQNAHVAV
                     TTSVDGFAAVWQHVLDRFFTRFAGAAAIEINDFGATPGVVLLRLEQALEALRA"
BASE COUNT          461 a         1201 c         1217 g          531 t
ORIGIN      
        1 cgcccacccc tacacctcga gcccgcggct cgcgattttc ggagcctcca acggcggcct
       61 gctggtgggt gcctgcatga cgcaacggcc ggagctctac ggcgccgtga ttgcggacgt
      121 gggcgtcatg gacatgctgc gcttcgacaa gttcgggcag ggcgcggggt gggagggaga
      181 cttcggctcg ccggacaacc cgcaggaatt caaggcgctc ttcgcgtatt cgccgctgca
      241 caatgtccgc gcgggtgtgc gctatccggc caccctggtc atcaccggcg atcacgacac
      301 gcgcgtgatg cctatgcact ccttcaagtt cgcggcggcg ctgcaggctg cgcaggcagg
      361 tcaggcgccg gtgctgctcg acgtggagct caactccggc cacggcggca gccagaccgt
      421 ctatcgcgcg atcgccctgc acgccgacat ctatacattc ctcgcgcgca atctcgcgat
      481 gaaataatgt aacgcgcatg gcgacagcgc tcgagccggg agcgggtccg cggagctgga
      541 ccacacgccc cgaggataag gcggcgcggc ttgcgggcct ggcgcagtac gtcgacggca
      601 agctggtgcg gcgcgccgac gtcgtagcgg tgctcgaggc gttgctgcgc cccggcgacc
      661 gcgtggcgct ggagggcaac aaccagaagc aggccgattt cctctcgcgc gcgctggtcg
      721 aggtcgaccc ccgcagggtc tacgacctgc atatgctgat ctcctccatc acgcgtcccg
      781 agcacctgac gctgttcgaa cgcggcatcg cccgccgggt ggagttctcc tacgccggtc
      841 cgcagagcct gcgggtggcg cagctcctcg aagacggcaa gctcgagatc ggcgcgatcc
      901 acacctacat cgagctgtat gcgcgcatgt tcgtcgacct gacgccgcac gtagtgctgg
      961 tgtgcgcgga aaaggcggat ctcgccggta atctctacac cggcccgaat accgaagaga
     1021 caccgacgct catcgaggcg gcggccttcc gccacggggt cgtgctcgtg caggtcaacg
     1081 aggtcgtcga tcggctgccg cgcgtcgacg tgccggggtc gtgggtcgac ctcatcgtgc
     1141 ccgccgatcg ccccttcgcg atcgaaccgc tcttcacgcg cgacccgcgc cagatcaatg
     1201 agctgcagat cctcatggga atgatggtga tccgcggcat ctacgagcgc cacggcgtga
     1261 ccgcactcaa tcacggcatc ggcttcgata ctgcggccat cgagctgctg ctgccgacct
     1321 acgcggcggc gctcggcttg cagggccgga tctgccgcaa ctggatcctg aatccccacc
     1381 cgaccctcat tcccgccatc gagagcggct gggtcgagag cgtgcactgt ttcggcggcg
     1441 aggtggggat ggaggattac gtgcgcgcac gccccgacgt gttcttcgtc ggcggcgacg
     1501 ggagtctgcg ctcgaaccgc gtgttgtgtc agctcgccgg ccagtacgga gtggacctgt
     1561 tcatcggctc gacgctgcag atggatgccg atgccaactc ctccaccgtc accttaggca
     1621 ggctcgccgg ctacggcggt gctcccaaca tgggccacga tccgcgcggg cgccggcacg
     1681 cgagtcccgc ctggctgagc ctgatcaccg ccgcagggcc ggtggtgcgc ggccgcaagc
     1741 tcgtcgtgca gctcgtcgag actttcagtc ggggcggtgt gcccgcgctg gtcgagtcgc
     1801 tggaagcggt ccaggtcggg cgcgacagcg gcttcgccgt cgctccggtc atgatctatg
     1861 gagacgacgt gagtcacgtg gtgagcgagg agggcatcgc ctatctctac aaggcgcagg
     1921 gccaggagga gcggcgggcc gcgctcgccg ccatcgccgg ggcgaccgag atcggccgcc
     1981 gcgcggaccc caggcgcagt gcgagcctgc gtgcccgcga gatcatcgcc tatcccgagg
     2041 atctcggtgt gcggcgcgcc gaggcgcgcc gctcgctcct cgcggcacgc agcatcgagg
     2101 atctggtcgg ctggtccggg gggctctaca agccaccggc ccggttccgc agctggtagc
     2161 agcatggcgc ccgctgtcca cctgcggctt gccgcgcgtc cgccccgccc agcgcagcgc
     2221 gcggcggccg agctcgccga gcgggcggtc cgcgcgctcg tcctcgaggc gctgctcacg
     2281 ccgaaacccg cgctggtcga ccggcgcggc tgcggtgctc atcgggatct cgatctcagg
     2341 aagctgctgt gctcggcacg tgcgctgcgc ggcaccttcc accgcatggc tctcgtcgcg
     2401 ggcacaagcg ccgcggacgt gcgcctgcgc gagcgcctgg gttggctcgg gcggcgcggt
     2461 gaggacgcca tgctcgcggc caccggtggc agcaacgcac accgcggcgc catctgggtg
     2521 ctcggcctca tggtcgcggc gctggcgctc gtggggactg atgcctcggc cgccgaggtg
     2581 gcggcgaccg cggccggcct ggcgcggctc ccggaccgcc gtgcgccgca cctgccgagc
     2641 cacggcgcgc gcgtgtgccg cgagttcggc gtggccggcg cacgtggtga ggcgcacgcg
     2701 ggattcccgc acgtgatcga agtgggactg ccggcgctcg cagcggcccg ccgccggggc
     2761 gcgcccgagt cctgcgcgcg gctcgacgcg ctgctcgcca tcatgtcgag cctcgatgac
     2821 acctgcctcc tgcaccgcgg cggctgggcg gcgctgcacg cggcgcgcag cggcgcccgc
     2881 cacgtcctcg cccttggagg cagcgccacg gcggcgggcg cggcggcgct gctcgcgctc
     2941 gacgccgagc tgctgcggca caacgcctcg ccgggcggct gtgcggatct gctcgccgcc
     3001 tgcctgtttc tcgacggtgc ggcgcacgag ctggagagcc aacactcatg gaacgcctga
     3061 gcttcatcta tccggcccgc gagtcggtgg cgcggcgtgt gcacgtcggg gtcgtcggct
     3121 cgggcgatct cgaggtgctg ctcgagccct cggcggacca gaacgcacat gtcgccgtca
     3181 cgaccagcgt cgacggattc gctgccgtgt ggcagcacgt cctcgatcgc ttcttcaccc
     3241 gtttcgcggg agcggcggcg atcgagatca acgatttcgg cgcgacgccg ggcgtcgtgc
     3301 tgctgcggct cgagcaggcg ctggaggcgc tgcgcgcttg agtgccgctg ccgggcgcgc
     3361 cggcctcgtg gcgcgggcga gcttcatcga gctcaacgcg cgcgagcggg
//
LOCUS       DATLKL010000127         3762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_170551, whole genome shotgun sequence.
ACCESSION   DATLKL010000127 DATLKL010000000
VERSION     DATLKL010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170551"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VM713_01685"
     CDS             <1..481
                     /locus_tag="VM713_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH83003.1"
                     /translation="AKALTNGAQPMGAVAVRADIYRTILDAAPEGMPEFFHGYTYTAH
                     PAACAAGLATLEIYRQEQLFARAKDTSGYFLDRMFALGELAAVADVRGFGMLAGVEVK
                     PGKAPGVRGHELQAKLYDRGLHVKTTGDVAIMAPAFIASRSDIDAMAEMLREAIAEF"
     gene            522..2969
                     /locus_tag="VM713_01690"
     CDS             522..2969
                     /locus_tag="VM713_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH83004.1"
                     /translation="MQSIPSHARVVIIGGGIVGCSVAYHLAKRGCSDVLLLERRQLTC
                     GTTWHAAGLVGQLRATHNLTRLAQYTTQLYEGLERETGQATGFRQVGSVAVAASEPRL
                     EELKRGASMARCFGLEVQILSAGEARERWPLLAIDDVVGAVYLPKDGRTNPVDTTQAL
                     ARGARMRGARIIEGVKVTGILRSHRRVAGVATEAGEIRADVVVNCAGMWAREVGALAD
                     VSVPLHAAEHFYIVTEPIPGLPPDLPILRDADACSYFKEDAGKLLVGWFEPRAKPWGE
                     EGIPESCAFEQLPADLAHIEPLFAGAMRRVPILETTGVQVFFNGPESFTPDDRYLLGE
                     APELAGLYVAAGFNSIGIQSAGGAGKVLADWIIDGHAPFDLWDVDIRRCAPFQRNRRY
                     LRDRTVEALGLLYAMHWPYRQPETARGVRKSALHERLAAAGACFGEVAGFERANWFGA
                     AGSDPRYEYSYGRQNWFGASALEHRAVREAVGLFDQSSFAKFVLKGGDAAAVLGQICA
                     NEVDVPVDRIVYTQWLNQRGGIEADVTVTRETEDSYLIVSSCATQTRDFHWLSRSIPE
                     GARAVAVDVSSAYAVLGLMGPKSRELLQSLTDADLSTAAFPFGTSRVIDLGYARVRAS
                     RITYVGELGYELYIPSEFAPAVYDVITAAGASFGLRLAGYHALNSLRLEKAYRHWGHD
                     ISDEDTPLEAGLAFAVAWDKPGGFLGRAALIEQRARGVRRRLALFALADADRLLYHNE
                     PIWRDGQLVGKVSSGMFGHTVGAALGLGFVVNRDEPVSDAWIASGRYEIEVAAERVPA
                     RVSLRAFYDPASTRVKC"
     gene            2966..>3762
                     /locus_tag="VM713_01695"
     CDS             2966..>3762
                     /locus_tag="VM713_01695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH83005.1"
                     /translation="MKSHARVVVIGGGAVGCSALYHLTRLGWTDVMLLERDELTADST
                     WHAAGNFPNFSTSWCLLKLQQYSTQLYARLGPEAGVDLSYHRTGSIRLAHSADRADEF
                     RHVAAQARALGLGFELLAPAEIRARHPFIELDDIRLGLWDPDDGDIDPAQLTQALAKG
                     ARDRGASIHRHTQVTGIRALTRGGWRLETNAGAIEAEYVINAAGYRGGEVAAMIGEYL
                     PIVSLSHQYLVTEDIAALVARAPARLPLVRDPDASYYLRQERHGLLLG"
BASE COUNT          545 a         1295 c         1329 g          593 t
ORIGIN      
        1 ggcgaaagcc ctcaccaacg gtgcccagcc gatgggcgcg gtggcggtgc gcgcggacat
       61 ttaccgcacg attctcgatg cggcgcccga gggaatgccc gagttcttcc acggctacac
      121 ctacacggcg catccggccg cctgcgcggc cggccttgcg acgctcgaga tctatcgcca
      181 ggagcagctg ttcgcccggg ccaaggacac ctccggctac ttcctcgaca ggatgtttgc
      241 cctgggcgag ctcgccgcgg tcgccgacgt gcgcggcttc ggcatgctgg ccggggtcga
      301 ggtgaaaccc ggcaaggcac cgggcgtgcg cggacacgag ctgcaggcga agctttacga
      361 tcgcggcctg cacgtgaaga cgaccggcga tgtcgcgatc atggcgccgg ctttcatcgc
      421 gagccggtcc gacatcgatg cgatggcgga gatgctgcgc gaggcgatcg ccgagttctg
      481 aagtccgcgt ccggcgtgcg gccggctctg gagggagggc ggtgcagagc attccgtcgc
      541 atgcgcgcgt cgtgatcatc ggcggcggca tcgtcggctg cagcgtcgcc tatcatctcg
      601 ccaagcgcgg ctgcagcgac gtgctgctgc tcgagcggcg gcagttgacc tgcggcacga
      661 cctggcacgc cgcaggactc gtcgggcagc tgcgcgccac gcacaatctc acccgcctcg
      721 cccagtacac cacgcagctc tacgaaggcc tcgagcgcga gaccgggcag gcgaccggct
      781 tccgacaggt ggggtcggtc gcggtggcgg ccagcgagcc gcggctcgag gagctcaagc
      841 gcggcgcctc gatggcgcgc tgcttcggcc tcgaggtgca gatcctctcc gcgggtgagg
      901 cccgcgaacg ctggccgctg ctcgcgatcg atgatgtggt gggcgcggtc tacctgccga
      961 aggacgggcg gaccaacccg gtcgacacca cgcaggcgct ggcgcgcggc gcgcgtatgc
     1021 gcggcgcccg catcatcgag ggtgtcaagg tgaccggcat cctgcgctcg caccggcgcg
     1081 tcgccggggt cgcgactgaa gcgggggaga tccgcgcgga cgtggtcgtc aactgcgccg
     1141 gcatgtgggc gcgcgaagtg ggcgcgctcg ccgacgtcag cgtcccgctg cacgcggccg
     1201 agcacttcta catcgtcacg gagccgattc ccggcctgcc gcccgacctg ccgatcctgc
     1261 gcgacgccga cgcctgctcc tacttcaagg aagacgccgg gaagctcctc gtcggctggt
     1321 tcgagccgcg ggccaagccc tggggcgagg aggggatccc ggagagctgc gcgtttgagc
     1381 agctgccggc ggatctcgcg cacatcgagc cgctgttcgc cggcgcgatg cgccgcgtgc
     1441 cgatcctcga gacgactgga gtccaggtgt tcttcaacgg ccccgagagc ttcactcccg
     1501 atgaccgcta cctcctgggc gaggcgcccg agctcgcggg gctctatgtc gccgcgggtt
     1561 tcaactccat cggcattcag tccgcggggg gcgccggcaa ggtgctcgcg gactggatca
     1621 tcgacgggca tgcgcccttc gatctgtggg acgtcgatat ccgccgctgc gcaccgttcc
     1681 agcgcaaccg gcgctacctc cgcgatcgga cggtcgaggc gctcgggctc ctctacgcca
     1741 tgcactggcc gtaccggcag ccggagaccg cgcgcggggt gaggaagtcc gcgctccacg
     1801 agcgcctcgc agcggcggga gcctgcttcg gggaagtggc gggcttcgag cgcgccaact
     1861 ggtttggggc tgcggggagc gacccgcgct acgagtacag ctatggccgg cagaactggt
     1921 tcggcgcttc cgcgctcgag caccgtgcag tgcgcgaggc ggtcggcctc ttcgaccagt
     1981 cgtcattcgc caagttcgtg ctcaagggag gcgacgccgc ggcggtactt ggacagatct
     2041 gtgccaacga ggtcgacgtg cccgtcgacc ggatcgtcta cacccagtgg ctcaaccagc
     2101 ggggcgggat cgaggcggat gtcacggtga cgcgtgagac ggaggattcg tacctgatcg
     2161 tcagctcctg cgcgacgcag acccgcgact ttcactggct gagccgcagc atccccgagg
     2221 gcgcgcgtgc cgtggccgtc gacgtgtcga gcgcctacgc ggtgctcggg ctcatgggcc
     2281 cgaagagccg cgagctcctt cagtcactca ccgacgcgga tctctcgacc gcggcctttc
     2341 ccttcggcac gtcccgcgtc atcgacctcg gctacgcgcg cgtgcgcgcg agccgtatca
     2401 cctacgtggg agagctcggc tacgagctct acattcccag cgagttcgcg ccagcggtgt
     2461 atgacgtgat cacggccgca ggcgcgtcct tcggtctgcg tctcgcgggt tatcacgcgc
     2521 tcaacagcct gcgcctggag aaggcctacc gccattgggg ccacgacatc agcgacgagg
     2581 acacgccgct cgaggcgggg ctcgccttcg ccgtcgcctg ggacaagccc ggcggtttcc
     2641 tcggccgcgc ggcgctcatc gagcagcgcg cgcgcggcgt gcggcgccgc ctcgcgctgt
     2701 tcgcgctcgc ggacgcggac cggctcctct atcacaacga gccgatctgg cgggacggcc
     2761 agctcgtcgg caaggtcagc tccgggatgt tcggccacac cgtgggtgcc gcgctcggct
     2821 tggggttcgt ggtcaaccgc gatgagcccg tgagcgatgc ctggatcgcc tcgggacgtt
     2881 acgagatcga agtcgccgcc gagcgcgtac cggcccgcgt ctcgctccgg gccttttacg
     2941 acccggcgag cacgcgggtg aagtgttgaa gagccacgcg cgggtcgtgg tgatcggcgg
     3001 gggcgcggtc ggctgcagcg cgctctatca tctgacgcgc ctcggctgga cggatgtcat
     3061 gttgctcgag cgcgatgagc tcacggcgga ctccacctgg cacgccgccg gcaacttccc
     3121 gaacttctcg acctcctggt gcctgctcaa gctccagcaa tactcgacgc agctctatgc
     3181 gcggctcggg cccgaggccg gcgtcgatct cagctaccat cgcaccggca gcattcgcct
     3241 ggcgcacagc gccgatcgcg cggacgagtt tcgccacgtg gccgcgcagg cgcgggcgct
     3301 ggggcttggg ttcgagctgc tggcacccgc agagatccgc gcgcgccacc cgttcatcga
     3361 gctcgatgac attcgcttag ggttgtggga tcccgacgac ggcgacatcg atccggcgca
     3421 gctcacgcag gcgctcgcca agggcgctcg cgacaggggc gcatccattc accgtcatac
     3481 gcaagtgacc ggaattcggg ctctcacgcg gggaggctgg cggctcgaga cgaacgcggg
     3541 cgcgatcgag gccgagtatg tgatcaacgc cgccggctac cgcggcggcg aagtggcggc
     3601 gatgatcggc gagtatctgc cgatcgtgtc gctctcgcac cagtacctcg tcaccgagga
     3661 tattgccgcg ctcgtggcgc gcgcgcccgc acggttgccg ctcgtgcgcg atcctgacgc
     3721 cagctactac ctgcgccagg agcggcatgg gctgctgctc gg
//
LOCUS       DATLKL010000128         2022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_57806, whole genome shotgun sequence.
ACCESSION   DATLKL010000128 DATLKL010000000
VERSION     DATLKL010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57806"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /gene="coaBC"
                     /locus_tag="VM713_01700"
     CDS             complement(<1..1154)
                     /gene="coaBC"
                     /locus_tag="VM713_01700"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HVH83006.1"
                     /translation="MQGKRILLGVTGGIAAYKSPDLVRRLRERGAEVQVVMTAAAREF
                     VTPTTFQAVSGRTVRTDLWDAAAEAAMGHIELARWADAVLVAPASADFLARLAAGLAD
                     DLLATLCLATEAPIAVAPAMNHVMWAHAATRANVAQLAARGVHVLGPASGDQACGETG
                     EGRMVEPLELAEQLAALLAAGGELAGQRVLITAGPTRERLDPVRFISNRSSGKMGFAV
                     AQAARAAGAQVVLVSGPVSVPTPPGVARIDVKSAADMLAAVLRELPGTDIFISTAAVA
                     DYRPVRAAQHKIKKTSDTLEVSMERTPDVLATVAALAKRPFVVGFAAETESVEQNARA
                     KLMKKNLDMIAANEVGDDKGFDSEENQLIVLARGARYELGRAGKLTLARGL"
     gene            1318..1992
                     /gene="radC"
                     /locus_tag="VM713_01705"
     CDS             1318..1992
                     /gene="radC"
                     /locus_tag="VM713_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HVH83007.1"
                     /translation="MAIRHWPEGERPREKLLERGAHALSDAELLAVLLGAGVRGQSAV
                     ELARTLIGELGSLRELLSAERARLLRRRGIGPARYAVLKAALELARRHFREALRAGPA
                     LAAPEATRTFLLAQLRDHPYEVFCCLYLDNRHRLIVFEELFRGTIDRAGVHPREVLRQ
                     TLAHNAAAVIFAHNHPSGVLEPSQADELITRRLKEALALVDVRVLDHFIIGDGLCYSF
                     SEHGLI"
BASE COUNT          273 a          729 c          705 g          315 t
ORIGIN      
        1 agtccgcgcg ccagagtaag cttgcccgcc ctgccgagct cgtagcgcgc gccgcgggcg
       61 agcacgatga gctggttctc ctcgctgtcg aagcccttgt cgtcgccgac ttcgttggcg
      121 gcgatcatgt cgagattctt cttcatgagc ttggcgcgcg cgttctgctc gaccgactcg
      181 gtctcggcgg cgaaccccac cacgaacggc cgcttggcaa gcgccgccac cgtggcgagc
      241 acgtccggtg tgcgctccat ggagacctcg agcgtgtcgc tggttttctt gatcttgtgc
      301 tgcgccgcgc gcaccggccg gtagtcggcg accgcggcgg tggagatgaa aatgtcggtg
      361 ccggggagct cccggagcac agcagccagc atgtcggcgg cgctcttgac gtcgatgcgc
      421 gcgactcccg gcggcgtcgg cacactcacc gggccgctca ccaggacgac ttgcgcgccg
      481 gctgctcgag ccgcctgcgc caccgcaaag cccatcttgc cggagctgcg gttgctgatg
      541 aagcgcaccg gatcgagccg ttcccgggtc ggtccggccg tgatcaggac gcgctggccc
      601 gcgagctcgc ccccggcggc gagcagcgcc gcgagctgct cggcgagctc gagcggctcg
      661 accatgcgtc cttcgcccgt ctcgccgcag gcttgatcgc cggaggcggg accgagcacg
      721 tgcacgccgc gcgcggcgag ctgggccacg ttggcgcgcg tcgccgcatg cgcccacatc
      781 acgtgattca tcgcgggcgc caccgcaatc ggcgcttcgg tcgcaagaca cagcgtcgcg
      841 agcagatcgt cggccagccc cgcagcgagc cgcgcgagaa agtcggcgct cgccggcgcg
      901 accagcacgg cgtccgccca gcgcgccagc tcgatgtgtc ccatggcggc ttccgcggcc
      961 gcgtcccaca ggtcggtgcg caccgtgcgg cccgataccg cctggaaggt ggtcggcgtc
     1021 acgaactcgc gcgcggcggc cgtcatcacc acctgcacct ctgcgccgcg ctcgcgcaga
     1081 cgccgcacga gatccgggct cttgtaggcg gcgatgccgc cggttacgcc gagaagaatg
     1141 cgcttgcctt gcatggaact ttgcctcgca aggggattat ctcaccgcgg cgcgcgcgga
     1201 gtgcaaacgg gcccgcgcaa ggtgcggaaa aacccgctac tcggagcgct tatgccctcc
     1261 tgccagggga gcttgagctc cttagactgg gcccacctgg ccttagaacc aaacgtgatg
     1321 gcgatccgcc actggcccga gggcgagcgg ccgcgggaga aactgctgga gcgcggcgcg
     1381 cacgcgcttt ccgacgccga gttgctcgcg gtgctgctcg gcgcgggtgt gcgcggtcag
     1441 tcggcggtgg agctcgcgcg cacgctgatc ggtgagctcg ggtcgctgcg cgagctcctc
     1501 agcgccgagc gcgcgcggct cctgcgccgg cgcggcatcg gcccggcgcg ctatgcggtg
     1561 ctgaaggcgg cgctcgagct cgcgcggcgg cattttcgcg aggcgctacg cgccggtccc
     1621 gcgctggccg cgccggaggc gacccggacg ttcctgctgg cgcagctgcg cgaccacccc
     1681 tacgaggtct tctgctgcct gtacctcgac aaccgccacc ggctgatcgt cttcgaagag
     1741 ctgttccgcg gcacgatcga tcgcgccggc gtccatcccc gcgaggtgct gcgccagacc
     1801 ctcgctcaca acgccgccgc ggtgatcttt gcgcacaacc acccctcggg cgtgctcgag
     1861 ccgagccagg cggacgagct catcacgcgg cgcctgaagg aagccctggc gctggtcgac
     1921 gtgcgcgtgc tcgatcactt catcatcggc gacgggctct gctactcgtt ctcggagcac
     1981 gggctcattt gaggggctcc ctagcggtcg tgcggcgggg gc
//
LOCUS       DATLKL010000129         1874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_69221, whole genome shotgun sequence.
ACCESSION   DATLKL010000129 DATLKL010000000
VERSION     DATLKL010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69221"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..486)
                     /locus_tag="VM713_01710"
     CDS             complement(322..486)
                     /locus_tag="VM713_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83008.1"
                     /translation="MAELVALQAEYAVWFEALPEPVQNSSTGEALQAIVDLDLDELIA
                     IQPPRGFGRD"
     gene            complement(681..1424)
                     /gene="istB"
                     /locus_tag="VM713_01715"
     CDS             complement(681..1424)
                     /gene="istB"
                     /locus_tag="VM713_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HVH83009.1"
                     /translation="MLVHPTLERLRALGLSGMADAFMELDNTPDAAGLSHQDWLGLLV
                     DREATSRENKRLARRLREARLRQSAAVEDVDLRAPRGLDRALFLQLSTCQWLRDHHHL
                     VVVGPTGIGKSWLACALGHKACREGFSVLYKRAPRLFSDLAQARGEGRLPRLLTTLER
                     IRLLIIDDWGPEPLDSQQRRDLLEIVDDRYDKGSLLITSQVPVSGWHEVIGDPTLGDA
                     ILDRIVHRAHRLDLRGESLRRRLAASPVT"
     gene            complement(1447..>1874)
                     /locus_tag="VM713_01720"
     CDS             complement(1447..>1874)
                     /locus_tag="VM713_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVH83010.1"
                     /translation="IFHRGKRVASHRRSLLPHRPSTVAEHMPSAHRRYRDWTHERIRR
                     EAEAVGPDTAVLIDVILRSRPHPEQGFRSCIGILGLVKRFGAGRVEIACARALALGTR
                     SYSSVAAILKNAQDRTPNSVPTDAPLLIHENIRGPGYYH"
BASE COUNT          300 a          549 c          709 g          316 t
ORIGIN      
        1 ccgcgacacg ctgcaggaga tgcgatgcgt gttgcacgcc catcgtgtgc cggggatggc
       61 tgcgcattcc ggtgatggcg atcggctatt ccagccgaag gcgatcacgc attccggtga
      121 tcgcgatcat ggccggtcga caggagcaag gcaggccatg acggctcagc ggcccgtagg
      181 cgctggcgtc agcaaaggtt gcaggattag ccccgtgtgg tcaagcgatc gggtatccgt
      241 gactgcctcc gtatctctgg atggccgcgc ttgcgcaggc tgggcgccgc tggaggatgg
      301 ggggttcatt acagcccgtc tctaatcgcg tccgaagccg cggggcggct gaatagcgat
      361 gagctcatcg aggtcgagat cgacgatggc ttggagagct tcgccggtgc tgctgttctg
      421 gacgggctcg ggcagtgcct cgaaccagac agcgtactcg gcttgtagcg cgacgagctc
      481 ggccacggca tcaacccagc gctggcgccg agatcgccgg tcggtgggcc ggcgatagcg
      541 aatgatgggg gcggtggcat gacgggcgcg atatcgggct tggcgctcgg ccgcggtgag
      601 cgccggctgg cccgtgggct tgggttgctg agacatttgt ggcttcctgg attgcgtcac
      661 gtaacggcgc gcaatgggcg tcatgtcacc gggctggcgg cgaggcggcg gcgcagggat
      721 tcgcctctga ggtcgaggcg gtgtgcgcgg tggacgatgc gatcgaggat ggcgtcgccc
      781 agggtggggt cgccgatcac ctcgtgccag ccggagaccg gcacttggct ggtgatcagc
      841 agcgatcctt tgtcgtagcg atcgtcgacg atctcgagca gatcgcgacg ctgttggctg
      901 tcgagcggct cgggtcccca atcgtcgatg atcagcagac gaatgcgctc gagcgtggtc
      961 aacagacgag gcagccggcc ttcgccgcgg gcttgggcaa gatcgctgaa cagccgcggc
     1021 gcgcgcttgt agagtaccga gaaaccctcg cgacaggcct tatgcccaag ggcacaggcc
     1081 agccacgact tgccgatgcc ggtcggcccg accaccacca gatggtgatg atcgcgcagc
     1141 cattggcagg tagaaagctg gaggaacagg gcgcggtcga gcccgcgggg ggcgcgcaag
     1201 tcgacgtctt cgacggcagc cgactgccgt aagcgggcct cgcgcaggcg tcgggcgagg
     1261 cgcttgttct cacggctggt tgcctcgcga tcgacgagca accctagcca gtcctggtgc
     1321 gagagcccgg cggcgtcggg cgtattgtcg agctccataa aggcgtcggc catgccggac
     1381 aagccgagtg cgcgcaagcg ctcgagggtg ggatggacca gcatcgggtc tcctttggtg
     1441 ccagggttag tggtagtagc cggggccgcg gatgttctcg tggatcagca gcggggcatc
     1501 ggtcgggacg ctattggggg tgcggtcctg agcgtttttc aggatcgcag cgaccgagct
     1561 gtaagagcgg gtgccgagcg ccagtgcgcg ggcgcaggcg atctcgacgc gaccggcgcc
     1621 gaagcgcttg accaggccga ggatcccgat acaggagcgg aacccctgct ccggatgcgg
     1681 ccgggagcgc aggatcacat cgatgagcac ggcggtgtcg gggccgaccg cttcagcctc
     1741 gcggcggatc cgctcgtgcg tccagtcgcg gtagcggcgg tgcgcactcg gcatgtgctc
     1801 ggctaccgtg ctgggccgat gcggcagcag actgcggcga tggctggcca cacgcttgcc
     1861 gcggtggaag atct
//
LOCUS       DATLKL010000130         2912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_204268, whole genome shotgun sequence.
ACCESSION   DATLKL010000130 DATLKL010000000
VERSION     DATLKL010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204268"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /locus_tag="VM713_01725"
     CDS             <1..1505
                     /locus_tag="VM713_01725"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVH83011.1"
                     /translation="PPPYSSTTLVQLSKTSTFAAGCDGVAANGTLYTDTAAEPSFVVN
                     PANSMNLVAGWQQNRWSTGGSQGLNLAASFDGGATWTLANAAFSRCTGGGSGNAGDYP
                     RASDVWLTAAPNGVVYALSLSFTCCALLAGSSSAMLVAQSQDGGRSWSAPVALIQDGE
                     QFFDDKGSITADPTDSNYVYVVWDRLNGPSAGPSYLALTADGGVSWTAARSIYDPGAA
                     NQTLGNQVAVLPGGVVLDVFTELDTAAGQTTALARVMQSLDHGTTWSAPITIAELVPV
                     GTSDPRTGRNVRDGSGIVSVSVGPGGVVYVAWQDGRFAGGAHDGIALTHSTDGGTTWT
                     APVEVNGDRAVQAFTPTVHVEASGVIAVTYYDLRNDAFAPFGALLADAWMVTSSDGTT
                     FTETHLSGPFDMDLAPDSAGLFVGDYEALSSAAGSLPLYVETDAGGAIRSDAFMAFPS
                     ATASAHSAVARAMGRFQALAAPAGLALTPAARRLIGQRMEARRVERRREP"
     gene            complement(1506..2414)
                     /locus_tag="VM713_01730"
     CDS             complement(1506..2414)
                     /locus_tag="VM713_01730"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HVH83012.1"
                     /translation="MLALIVSLLPAAGVAAGRDSAALESEQAAIARWRSERVQSLTSD
                     DGWLTLAGLFWLKEGENTFGRGSGNALVLDNAALADTAGTFVLSGEQVRFRARPGGGV
                     TRDGQPVTELDLAPDTSGHPTVLSCGALRFYVIERAGHLGVRVRDLNNPHRSGFRGLE
                     YFPVSTDWVVSARFERYQPVRHISIMNILGMELEMESPGAVVFSKDGREWRLDTVLEE
                     PGDTELFIMFADATSGRETYGAGRFLYIPIPAGDTVPVDFNRAYNPPCALNDFATCPL
                     PPWQNRLKLRVTAGEKKYAGGTDHHF"
     gene            complement(2506..>2912)
                     /locus_tag="VM713_01735"
     CDS             complement(2506..>2912)
                     /locus_tag="VM713_01735"
                     /inference="COORDINATES: protein motif:HMM:NF023689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3612 domain-containing protein"
                     /protein_id="HVH83013.1"
                     /translation="ISVLRDGERALLYCCHSIRVRDMAGNPHVLSVGVDLVPALTANG
                     VDGAATVGAILRECLHRHGEARIPKEAAEELGAVANVLNIAWIGDALTQTARIICPRS
                     NRCPRRERCEHAPSSRAREIADVREEILGREP"
BASE COUNT          416 a         1008 c         1023 g          465 t
ORIGIN      
        1 cacccccgcc ctacagctcg acgacgctgg tgcagctctc gaagacctcg accttcgccg
       61 cgggctgtga cggcgtggcg gccaacggga cgctttacac cgacaccgcc gccgagccgt
      121 ccttcgtcgt caaccccgcc aactccatga acctggtcgc gggctggcag cagaaccgct
      181 ggtcgaccgg cggctcacag ggtctgaacc tcgctgcctc attcgacggc ggcgcgacct
      241 ggacgcttgc gaacgccgcc ttctcgcgct gcaccggcgg cggcagcggc aacgccggcg
      301 actacccgcg cgcgagcgac gtgtggctca cggccgcgcc gaacggcgtg gtctacgcgc
      361 tgtcactgtc cttcacctgc tgcgcgctgc ttgcgggctc gtcgagtgcg atgctggtcg
      421 cgcagtcgca ggacgggggc cgaagctgga gcgcgccggt ggcgctcatc caggacggtg
      481 agcagttctt cgacgacaaa ggctcgatca ccgccgaccc caccgactcg aattacgtct
      541 acgtggtatg ggaccgcctc aacgggccga gcgccgggcc gagctacctt gcgctcactg
      601 ccgatggcgg ggtcagctgg acggccgcgc gcagcatcta cgatccgggc gccgccaatc
      661 agacgctcgg caatcaggtc gcggtgctgc caggtggtgt ggtgctcgat gtcttcaccg
      721 agctcgatac cgccgcgggg cagaccacgg cgctcgcgcg ggtgatgcag tcgctcgatc
      781 acggcacgac ctggtccgcg ccgatcacga tcgcggagct cgtgcccgtc ggcacgagcg
      841 atccgcgcac cggccggaac gtgcgcgacg gctccggcat cgtctcggtg agcgtaggtc
      901 cgggcggcgt cgtctatgtc gcctggcagg acgggcgctt cgcgggcggc gctcacgatg
      961 gaatcgcgct cactcattcc accgacggcg gcacgacctg gactgcgccg gtggaggtga
     1021 acggcgaccg cgcggtgcag gcgttcacgc ccaccgtgca cgtcgaggcg agcggcgtca
     1081 tcgccgtgac ctactacgac ctgcggaacg atgcgttcgc gccgttcggc gcgctgctcg
     1141 ccgacgcctg gatggtgact tcgagcgacg gaaccacttt cacggagacc catctctcgg
     1201 gacctttcga catggatctc gcgccggatt cagccggact cttcgtcggc gattacgagg
     1261 cactctcgag cgcggccggc tccctgccgc tttacgttga gaccgacgcc ggcggcgcga
     1321 ttcgcagcga tgccttcatg gcgttcccct cggccacggc ctcggcgcac tccgcggttg
     1381 cccgggccat ggggcgcttt caggccctgg cggcgccggc gggtctcgct cttacgcccg
     1441 ctgcgcggcg actcatcggg cagcgcatgg aggcgcggcg cgtggagcgg cggcgcgagc
     1501 cgtagttaga aatggtggtc ggttccgccg gcgtactttt tctcgcccgc cgtgactctg
     1561 agcttcagac ggttctgcca gggcggtaac gggcaggtcg caaagtcgtt gagcgcgcac
     1621 ggcgggttgt atgccctgtt gaagtcgacc ggcacggtgt cgcccgccgg gattggaatg
     1681 tagagaaaac ggccggctcc gtaggtctcg cggccgctgg tcgcatcggc gaacatgatg
     1741 aagagctcag tgtcgccggg ctcctcgagc acagtgtcga ggcgccactc gcgcccgtcc
     1801 ttgctgaaga ccaccgctcc cggagactcc atctcgagct ccatgccgag gatgttcatg
     1861 atgctgatgt ggcgcaccgg ctggtagcgc tcgaagcgcg cactgactac ccagtcggtg
     1921 ctgacgggaa agtactcgag gccgcggaag ccgctgcggt gcgggttgtt caggtcgcgg
     1981 acgcgcacgc cgagatggcc ggcccgctcg atgacgtaga agcgcagcgc gccgcagctg
     2041 agcaccgtgg gatggccgct cgtgtccggg gcgaggtcga gctcggtgac cggctggccg
     2101 tcccgggtga cgccgccgcc gggacgggcg cggaagcgca cctgctcgcc gctcagcacg
     2161 aacgtgccgg cggtatcggc gagcgcggcg ttgtcgagga ccagggcgtt cccggagccg
     2221 cggccgaagg tattctcgcc ctccttgagc cagaagagtc ccgcgagcgt cagccagccg
     2281 tcgtcgctcg tgagactctg gacccgctcg ctgcgccagc gggcgatcgc ggcctgctca
     2341 ctctcgagcg ccgcgctgtc ccgtcccgcc gcgacgccgg cggcgggcaa gagtgacacg
     2401 atgagcgcca gcaccggggg caatccgcgg gtgctacgcg cgtgctgcct gtgtcttacc
     2461 gtcatggagc cctccgccag tggcgcgagg ctgcctatca tgccatcacg gttcgcgtcc
     2521 gaggatctcc tcgcgcacgt cggcgatctc acgggcgcgg gacgagggcg catgctcgca
     2581 gcgttcccgc cggggacagc ggttgctgcg cgggcagatg atccgcgcgg tttgtgtgag
     2641 cgcatcgccg atccaggcga tgttgagcac gttggctacc gcgccgagct cctcggccgc
     2701 ttccttcggt atacgcgcct cgccgtgccg gtgcaggcac tcgcgcagga tggcgccgac
     2761 cgtcgccgcg ccgtcgacgc cgttggcggt gagcgcgggc acgagatcca cgccgaccga
     2821 aagcacatgc gggttgccgg ccatgtcgcg cacgcggatc gaatggcagc agtacaggag
     2881 cgcgcgctcc ccgtcgcgca gcaccgagat ct
//
LOCUS       DATLKL010000131         2011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_91733, whole genome shotgun sequence.
ACCESSION   DATLKL010000131 DATLKL010000000
VERSION     DATLKL010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91733"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..607)
                     /locus_tag="VM713_01740"
     CDS             complement(128..607)
                     /locus_tag="VM713_01740"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HVH83014.1"
                     /translation="MDAAPLAIEIRPNCSLTVRGAWLFFASACVMPFGMGGFLALKGF
                     WPILPFAGLEMALLAWALKVSLERRFHSQTITVTESDVAIESRGRSGRERIVFPRHWA
                     QVKLRRPAASLHPSRLTIESHGRQCELGSFLTEEERRGLALRLQRLIGRVNQSPAWP"
     gene            complement(651..1352)
                     /gene="bioD"
                     /locus_tag="VM713_01745"
     CDS             complement(651..1352)
                     /gene="bioD"
                     /locus_tag="VM713_01745"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HVH83015.1"
                     /translation="MSSFFVTGTDTGVGKTVVACALARAFAAAGERVAVMKPIASGAE
                     RTAEGLRNADALALIKAANVPLSYPQVNPYCFEPAISPHIAAEEAKIELDTGTIKAGF
                     EALKAISDRVVIEGAGGWLAPINARASMGDLARALGVPAVLVVGLRLGCLNHADLTRR
                     AIASDGVAFAGWVANRLSEPMEREAENLATLAQRLGAPPLALVPYAPARAASLVLAEG
                     AARLARATKMSGISA"
     gene            complement(1349..>2011)
                     /locus_tag="VM713_01750"
     CDS             complement(1349..>2011)
                     /locus_tag="VM713_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVH83016.1"
                     /translation="IALDIAPGMLREARRNQRLWRRFALVCTDARRLPLAAASADLVF
                     SSLVLQWCEPLEQALAEVRRVLAPGGFFAFSTCGPDTLRELRAAWAQVDGLTHVNRFR
                     DMHEVGDALTHSGLAEPVLDVDRIELGYTDALALMHDLKAIGAHNVTAGRPRTLLGRS
                     RLKRLTEAYEAFRRGATLPATYEVIYGACWGAAGAPAAAASAGEVRIAPGSIRRPEPR
                     RR"
BASE COUNT          318 a          703 c          707 g          283 t
ORIGIN      
        1 ggcggcggcg agggcgagtc gggccagcgt cttagtcatt ttcctttgcc ttacagcgcg
       61 cgcccgtcat ggcgggtgca gcgcgaaaaa attccggagc ggcctctcga cttcccgacc
      121 gcagccctca cggccaggcg ggcgactggt tgacgcggcc aatgagacgc tgcaagcgca
      181 gggccagtcc gcgtcgctcc tcttcggtca gaaagctgcc cagctcgcac tggcgaccgt
      241 gcgactctat ggtgagccgg ctcgggtgca ggctggcggc agggcggcga agtttaacct
      301 gcgcccaatg ccgcgggaac acgatccgct cgcggccgct gcgcccacgc gattcgatgg
      361 cgacgtccga ctcggtgaca gtgatggtct gggagtgaaa gcggcgctcg agcgagacct
      421 tgagcgccca ggcgaggagc gccatctcga gtcccgcgaa cggcaggatc ggccagaagc
      481 ccttgagcgc gaggaaccct cccatgccga agggcatcac gcaggcgctc gcgaaaaaca
      541 gccatgcgcc gcgcacggtg agggagcagt tcgggcggat ctcgatcgcg agcggcgcgg
      601 cgtccatggc ttgccttgcg gatggtaacg cgcagctgcg gcgattcaaa tcaagcggaa
      661 attcccgaca ttttcgtggc tcgggcgagc cgcgccgccc cttccgcgag caccagcgat
      721 gcggcgcgcg caggtgcgta gggcactagt gcgagcggcg gcgccccaag ccgctgcgcg
      781 agggtcgcga gattctccgc ctcgcgctcc atcggctcac tcaagcggtt ggcgacccag
      841 ccggcgaagg ccacgccgtc gctcgcgatc gcacggcgcg tgaggtcggc gtggttgagg
      901 cacccgaggc gcagccccac gaccagcacg gccggcacgc cgagcgcccg cgcgaggtct
      961 cccatgctcg cgcgggcgtt gatcggggcg agccagccgc ccgccccttc gatcaccacc
     1021 cgatcagaga ttgccttgag cgcctcaaat ccggccttga tcgtgccggt atcaagctcg
     1081 atcttggctt cttctgcggc gatatgcggt gaaatggctg gctcgaagca atacgggttc
     1141 acctgcgggt aggagagcgg cacgttcgcc gctttgatca acgccagcgc gtcggcattg
     1201 cgcaggccct ccgccgtgcg ctccgcgccg gatgcaatcg gcttcatgac ggcaacgcgc
     1261 tcgccggcgg ccgcgaaggc ccgcgccagc gcacaggcca ccaccgtctt gccgacgccg
     1321 gtatcggtac cggtgacgaa aaagctgctc accgccggcg cggctccgga cggcgaatcg
     1381 accccggggc gatgcgcacc tcgcccgcgc tcgccgcggc ggcgggcgca cccgccgcac
     1441 cccagcacgc gccgtagatc acctcgtagg tcgcggggag cgtggcgcca cgccggaagg
     1501 cctcgtaggc ctcggtcagg cgcttgaggc gcgagcggcc gaggagcgtg cgcgggcgcc
     1561 ccgcggtcac gttgtgcgcg ccgatcgcct tcaagtcgtg catcagcgcg agcgcgtcgg
     1621 tgtagccgag ctcgatgcga tcgacgtcga gcacgggctc cgcgagcccg ctgtgggtga
     1681 gtgcgtcgcc gacctcgtgc atgtcgcgga aacgattcac gtgcgtcaag ccgtcgacct
     1741 gcgcccaggc ggcccgcagc tccctcagtg tgtcggggcc gcaggtgctg aaggcaaaga
     1801 agccgccggg cgcgagcacg cgccgcactt cggcgagcgc ctgctcgagc ggctcgcacc
     1861 actggagcac caggctcgag aacacgaggt cggcgctcgc cgctgcgagc ggcagccgcc
     1921 gcgcatcggt gcagacgagc gcaaagcgcc gccacagcct ttggttgcgg cgcgcctcac
     1981 gcagcatccc gggcgcgata tcgagcgcga t
//
LOCUS       DATLKL010000132         3390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_193140, whole genome shotgun sequence.
ACCESSION   DATLKL010000132 DATLKL010000000
VERSION     DATLKL010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193140"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VM713_01755"
     CDS             <1..1001
                     /locus_tag="VM713_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HVH83017.1"
                     /translation="GLKRIRAGRCVPGIRALLAIAGRSVADLTAADLAFGVAPRLNAA
                     GRIDDMTIGIQCLLADDPAAAQQLALRLDELNEERRAIEARMQQDALAAVRRLAEPGP
                     DAIERSGVCLFDESWHQGVVGLVASRVKERVRRPVIAFAAADPATLRGSARSVAGVHI
                     RDVLDRIAVRHPGLISRFGGHAMAAGLTLERARLDEFARAFDAEVARWTGARAAADAV
                     ETDGELALAEIALATAEALRAGGPWGQAFPEPSFDGVFRIGSARVIGERHLKMWVEPE
                     SSGRSFDAVVFNHFEAGAPGALPEGLVQLVYRLDVNEYQGERRLQLLVDHLLPVAR"
     gene            1086..2147
                     /gene="prfB"
                     /locus_tag="VM713_01760"
     CDS             1086..2147
                     /gene="prfB"
                     /locus_tag="VM713_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HVH83018.1"
                     /translation="MSASASARFGGFFEYDRKLERLEEVARELEDPAVWSDPARAQEL
                     GRERARLTSDVGEIDRTSRGVGEAAELLELAEAEGDAGAAQDLERDAQRLEEAVRRLE
                     LKRMFRGEMDSHSAFLDIQAGAGGTEAQDWAQMLLRMYLRWGAARGFRCDVIDTSPGE
                     VAGIKSASVEVQGEYAYGWLRTETGVHRLVRKSPFDSGNRRHTSFASVFVSPEVDDDI
                     EIEVNPADLKTDVYRSSGAGGQHVNKTESAVRITHLPTGIVVACQNERSQHKNRSTAM
                     KMLKAKLYELEVNKRNAAARVLEDSKSDVSWGNQIRSYVLDQSRIKDLRTGVEVGNTQ
                     AVLDGDLDQFMEASLKAGV"
     gene            2152..>3390
                     /gene="lysS"
                     /locus_tag="VM713_01765"
     CDS             2152..>3390
                     /gene="lysS"
                     /locus_tag="VM713_01765"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HVH83019.1"
                     /translation="MKSSGNNSTGSDDDGGDENKLIAERRSKLAELRRGAGTGGAFPN
                     DFRRDALAGELQAAYGERSGEWLEANPTRVSVGGRLMFKRVMGKASFAKIADRTGQIQ
                     LYLQQEALGPVYEAFKGWDVGDILGGSGVLFRTRTGELSVRADALRLLVKSLRPLPDK
                     WHGLADTETRYRRRYVDLIMSDASREVFRARARIVRYLRDFLDALGFLEVETPMMQPI
                     PGGAAARPFRTHHNALDLDMYLRIAPELYLKRLIVGGLDRVYEINRNFRNEGLSTQHN
                     PEFTMLELYLAWSDYRDIMDWVEKATRGLADTLNGRLSLTYRGRPYDLAQPFRRLTVE
                     QAIIDNNPGIDPLSLRDLTYLRKRCEQLGILAKPEDGPGKLQIELFEKTAEAKLLDPT
                     FVYAYPVEVSPLSRPNDADPF"
BASE COUNT          543 a         1169 c         1172 g          506 t
ORIGIN      
        1 agggcctcaa gcgcatccgc gccggacgct gtgtgccggg catccgcgcg ttgctcgcga
       61 tcgccgggcg cagcgtcgcg gatctgacgg cggcggatct tgctttcggc gtcgcgccgc
      121 ggctgaacgc cgccggccgc atcgacgaca tgaccatcgg catccagtgc ctgctggccg
      181 acgatccggc cgccgcgcag cagctggcgc tgcggctcga cgagctcaac gaggagcgcc
      241 gtgcgatcga ggcgcgcatg cagcaggacg cgctcgctgc ggtgcgccgc ctggccgagc
      301 ccggtcccga cgccatcgag cgcagcggcg tctgcctctt cgatgagagc tggcaccagg
      361 gcgtcgtcgg gctggtcgcg agccgcgtca aggagcgggt gcgccgcccg gtgatcgcct
      421 tcgcggccgc cgatccggcc acattgcgcg gctcagcccg ctcggtggcg ggcgtgcaca
      481 tccgcgacgt gctcgatcgc attgcagtcc gtcatcccgg gctcatcagc cgcttcggcg
      541 gccacgccat ggcggcgggc ctcacgctcg agcgggcgcg gctcgatgag tttgcgcgtg
      601 ccttcgacgc ggaggtggcg cgttggacgg gcgcgcgcgc cgctgcggat gccgtggaaa
      661 ccgacggcga gctcgcgctc gcggagatcg cgctcgctac cgccgaggcg ctgcgcgccg
      721 gcggtccctg ggggcaggcg tttcccgagc cgtccttcga cggcgtgttc cgcatcggca
      781 gcgcgcgcgt catcggcgag cggcacctga agatgtgggt cgagcccgag tcgagcggcc
      841 gctccttcga tgcggtcgtc ttcaaccact tcgaggcggg cgccccgggc gcgctgcccg
      901 aggggctggt gcagctcgtc taccggctgg acgtcaacga ataccagggc gagcggcggc
      961 tgcagctgct ggtcgatcac ctgctgccgg tcgctcgctg aaggcaccac cccggtgtta
     1021 gcatccccgg catcacacgt gcaggacccc catggaagtc aatctgctca ggcgtcatct
     1081 caaggatctc ggcgagcgca tcggctcgct tcgggggttt ctttgagtac gaccgcaagc
     1141 tcgagcgcct cgaagaggtg gcacgcgagc tggaggatcc ggcagtgtgg tccgacccgg
     1201 cacgcgccca ggagctcggg cgcgaacgcg cccggctgac gagcgacgtc ggcgagatcg
     1261 accgcaccag ccgcggcgtc ggcgaagccg ccgagctcct cgagctcgcc gaagccgagg
     1321 gcgatgcggg cgccgcgcag gacctcgagc gcgacgcgca gcgtctcgag gaggcggtgc
     1381 gccgcctcga gttgaagcgc atgttccgcg gcgagatgga ttcgcacagt gcctttctcg
     1441 acatccaggc gggcgcgggg ggtaccgagg cgcaggactg ggcgcagatg ctgctgcgca
     1501 tgtatctgcg ctggggtgcc gcgcgcggct ttcgctgcga cgtgatcgat acgagcccgg
     1561 gggaggtagc cggcatcaag agcgcgagcg tcgaggtaca gggcgagtac gcctacggct
     1621 ggctgcgcac cgagaccggc gtgcaccggc tggtgcgcaa gtcgcccttc gactcgggca
     1681 accgccgcca cacctccttc gcctccgtgt tcgtctcgcc cgaggtcgac gacgatatcg
     1741 agatcgaggt gaatccggca gacctgaaga ccgacgtgta ccgctcatcg ggcgccggcg
     1801 gccagcatgt caacaagacc gagtcggcgg tgcgcatcac gcacctgccg accggtatcg
     1861 tggtcgcctg ccagaatgag cgcagccagc acaagaaccg ctccacggcg atgaagatgc
     1921 tcaaggcaaa gctctacgag ctcgaagtca acaagcgcaa tgcggccgcg cgcgtgctcg
     1981 aggactcgaa gtccgacgtc agctggggca atcagatccg ctcctacgtg ctcgaccagt
     2041 cgcgcatcaa ggatctgcgc accggcgtcg aggtgggcaa cacccaggcg gtgctcgacg
     2101 gcgatctcga tcagttcatg gaggcctcac tcaaggcggg cgtctagctg aatgaagagc
     2161 agcggcaata actccaccgg ctccgacgac gacggcgggg atgagaacaa gctgatcgcc
     2221 gagcggcgca gcaagctcgc cgagctgcgc cgcggtgccg gcacgggcgg ggcctttccg
     2281 aacgacttcc gccgcgacgc gctcgccggt gagctgcagg ccgcttacgg cgagaggagc
     2341 ggcgagtggc tcgaggccaa cccgacgcgc gtcagcgtcg gcggccgcct catgttcaag
     2401 cgcgtcatgg gcaaggcgag cttcgccaag atcgccgatc gcaccggaca gattcagctc
     2461 tacctgcagc aagaggcgct cggcccggtc tacgaggcct tcaagggctg ggacgtgggc
     2521 gacatcctcg gcggcagcgg cgttctgttc cgcacgcgca ccggcgagct gtcggtgcgc
     2581 gccgatgcgc tgcgcctgct ggtcaagtcg ctgcggccgc tgcccgataa atggcacggt
     2641 cttgccgaca ccgagacgcg ctaccgccgc cgctacgtgg acctcatcat gagcgatgcg
     2701 agccgcgagg tgttccgcgc ccgcgcgcgc atcgtgcgct acctgcgcga cttcctcgac
     2761 gcgctcggtt ttctcgaggt cgagacgccg atgatgcagc cgattccggg cggcgccgcg
     2821 gcgcggccgt tccgcacgca ccacaacgcg ctcgacctcg acatgtacct gcgcatcgcg
     2881 ccggagctgt acctgaagcg cctgatcgtg ggcggcctcg atcgcgtcta cgagatcaac
     2941 cgcaacttcc gcaacgaggg cctctcgacc cagcacaacc ccgagttcac catgctggag
     3001 ctgtatctcg cctggtccga ctaccgcgac atcatggact gggtggagaa ggcgacgcgc
     3061 ggactcgccg acacgctcaa cggccggctg agcctcacct accggggccg gccttacgac
     3121 ctcgcgcagc cgtttcgccg cctgacggtc gagcaggcaa tcatcgacaa caatccgggc
     3181 atcgatccgc tctcgctgcg cgatctgacc tatctgcgca agcgctgcga gcagcttggc
     3241 atcctagcca agccggagga cggcccaggg aagctgcaga tcgagctgtt cgagaagacc
     3301 gcggaagcaa agcttctcga ccccaccttc gtctatgcct acccggtgga ggtctcgccg
     3361 ctgtcacggc cgaacgacgc ggatccattc
//
LOCUS       DATLKL010000133         1575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_159359, whole genome shotgun sequence.
ACCESSION   DATLKL010000133 DATLKL010000000
VERSION     DATLKL010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159359"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..419
                     /gene="rpsP"
                     /locus_tag="VM713_01770"
     CDS             165..419
                     /gene="rpsP"
                     /locus_tag="VM713_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010630215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HVH83020.1"
                     /translation="MVTIRLTRRGARNQPFYHVVVTDSRKRQGGPSLEHVGFFNPIAR
                     RGEIKVKLDLPRIDYWVGKGAHPSDRVRTLVASYRKQATA"
     gene            465..968
                     /gene="rimM"
                     /locus_tag="VM713_01775"
     CDS             465..968
                     /gene="rimM"
                     /locus_tag="VM713_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HVH83021.1"
                     /translation="MSWIELGRLGAPYGTKGWLHVDSYTDPPDGLLKYREWALRLASG
                     ERVTRRVTTGRGHGRGLVAQLEGVASREGAARLTGAIVEVERTALPPAGEQQYYRADL
                     VGLAVRNLEGVALGTVSHFVDVPTGAVMVTKEPGGREHWVLASPKHLRTVDLAQRTVV
                     VDWPAEL"
     gene            965..>1575
                     /gene="trmD"
                     /locus_tag="VM713_01780"
     CDS             965..>1575
                     /gene="trmD"
                     /locus_tag="VM713_01780"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HVH83022.1"
                     /translation="MRIEVVTLFPRMIAGALEYGVLGRAIERGLVTVGTEDPRAYTQD
                     AHRTVDDRPYGGGPGMVMKAEPLLAAIRAAQARLPPGSVRICLSAQGEPFDARAARSL
                     ATLPGMLLVAGRYEGIDERVIELGIDRELSVGDYVLSGGELPALTVIDAVARLLPGAL
                     GDERSSEEASFAQGLLDWPHYTRPEVFEGRTVPPVLLSGDHAG"
BASE COUNT          237 a          496 c          598 g          244 t
ORIGIN      
        1 cccggctcgc cggcgggctc cacgactccc agtgccgccc atgcggtgac ccgcgaggtc
       61 tggaaaaact ggcgcagaat tgcagcgttg cgtagaatcc gcgctctttt ttgggcccct
      121 cgggatgagg cgacctcctt agctggaacg agagcgatac gaccatggtt accattcgcc
      181 tgacgcggcg tggggcgcgc aatcagccct tctatcacgt cgtcgtgacc gacagccgca
      241 agcgccaagg cggcccgagc ctcgagcacg tgggattctt caacccgatc gcccggcggg
      301 gtgagatcaa ggtaaagctc gatctgccgc gcatcgacta ctgggtcggg aagggggcac
      361 acccctcgga ccgcgtaagg acgctcgtgg cgtcctaccg caagcaggct acggcctgaa
      421 gccctgagac ggcactcgcg gcgacgtgcc gagcgtgagc agccgtgtcc tggatcgagc
      481 ttgggcgcct gggcgctccc tacggcacta agggctggct gcacgtcgat tcctataccg
      541 atccgccaga cgggctgctg aaataccgcg aatgggcctt gaggctcgcg agcggcgagc
      601 gggtcacgcg gcgggtcacg acggggcggg gccacggccg cggtctggtc gctcagcttg
      661 agggcgtggc gagccgtgaa ggcgccgcca ggctcaccgg agcgatcgtc gaggtagagc
      721 gcacggcgct tccgcccgcg ggcgagcagc agtactaccg cgcggacctc gtgggactcg
      781 cggtgcgcaa cctcgagggc gtggcgctgg ggaccgtgag ccacttcgtc gatgtgccga
      841 ccggagcggt gatggtgacg aaggagccgg gcggacgtga gcactgggtg ctcgcctcac
      901 cgaagcatct gcgcacggtg gacctggcgc aacggacggt ggtggtggac tggccggccg
      961 agctatgagg atcgaagtcg tcacgctgtt tccgcgcatg atcgcgggag cattggagta
     1021 cggggtgttg ggccgcgcca tcgagcgcgg gctcgtcacc gtcggcaccg aggatccgcg
     1081 cgcgtacacg caggacgcgc accgcacggt cgacgaccgg ccttacgggg gcggtccggg
     1141 catggtgatg aaggctgagc cgctgctcgc ggcgatccgc gcggcgcagg cacggctgcc
     1201 gccggggagc gtgcggatct gcctgagcgc tcagggcgag ccgttcgatg cgcgcgcggc
     1261 gcgctcgctg gcgacgctgc cgggcatgct gctggtggcg gggcgctacg aggggatcga
     1321 tgagcgcgtg atcgagctcg ggatcgatcg cgagctctcg gtcggcgact acgttctttc
     1381 cggcggggag ctgccggcgc tcacggtgat cgacgccgtg gcgcggttgc tgcccggggc
     1441 actgggcgat gagcgctcga gcgaggaggc ttcgttcgcg cagggtctcc tcgactggcc
     1501 gcattacacg cggccggaag tgttcgaggg ccgcacggtg ccgccggtgt tattgtcggg
     1561 cgaccacgcc ggcat
//
LOCUS       DATLKL010000134         1789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_91757, whole genome shotgun sequence.
ACCESSION   DATLKL010000134 DATLKL010000000
VERSION     DATLKL010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91757"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VM713_01785"
     CDS             complement(<1..531)
                     /locus_tag="VM713_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVH83023.1"
                     /translation="MRYRILTLNQISPRGLERLPDELYEVGADIAEPDAILVRSAELD
                     AATVPATVRAIGRAGVGVNNIPVEEMSRRGVAVFNSPGANANAVKELVLAGLLLAARN
                     LGPAWLFTRALRGDDEAIEREVERGKKQFVGFELPGRTLGVVGLGAIGVEVANSALAL
                     GMKVVGYDPQITVQRAW"
     gene            complement(541..1623)
                     /gene="serC"
                     /locus_tag="VM713_01790"
     CDS             complement(541..1623)
                     /gene="serC"
                     /locus_tag="VM713_01790"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HVH83024.1"
                     /translation="MRAFNFAAGPAMLPVEVLEQAREELTDWQGLGASVMEVSHRGKP
                     FLAMAREAESDLRELLRVPANYRVLFMQGGGAAQSSVVPLNPAAVDAGVDYLVTGHWS
                     KRALAEAQRLVKRVNVAADEAASNYTTAPGQAALRLTAGAAYVHYCPNETIGGVEFPY
                     VPETGGVPLVADMSSTILSRPIDVRGFGLIYAGAQKNIGPSGLVVVIVREDLLDRARK
                     DVPAVWRYRSVADEGSMFNTPPTFSWYLAGLVFKWLKARGGLAAMGERNRAKAERLYA
                     AIDGSGFYRNPVAPECRSWMNVPFTLADSALEGAFAAEARAAGLINLEGHRSVGGMRA
                     SIYNAMPLEGVEALIAFMKDFERRKG"
BASE COUNT          270 a          641 c          612 g          266 t
ORIGIN      
        1 ccaggcgcgc tgcacggtga tctgcgggtc gtagcccacg accttcatgc cgagcgcgag
       61 cgccgagttc gccacctcga ccccgatcgc cccgagcccg accaccccga gcgtgcggcc
      121 ggggagctca aagccgacga actgcttctt gccgcgctcg acttcccgct cgatcgcctc
      181 atcatcgccg cgcagggcac gcgtaaagag ccacgcgggt ccgaggttgc gcgccgcgag
      241 caataggccc gcgagcacca gctccttgac ggcgttggcg ttggcgcccg gggagttgaa
      301 cacggcaacg ccgcgccggc tcatctcctc caccgggatg ttgttgacac cgaccccggc
      361 gcgcccgatg gcgcgcacgg tggcggggac ggtcgcggcg tcgagctcgg ccgagcgcac
      421 caggatcgcg tccggctcgg cgatgtcggc cccgacttcg tagagctcgt ccggcagccg
      481 ctcgaggccg cggggactga tctggttcag ggtcaggata cggtagcgca ttggcgcggc
      541 tcagcccttg cggcgctcga aatccttcat gaaagcgatc agcgcctcca ccccctcgag
      601 cggcatggcg ttgtagatac tggcgcgcat gccgccgacc gagcgatggc cttcgaggtt
      661 gatgagcccg gcggcgcgcg cctcggcggc gaaggcgccc tcgagcgcgg aatcggcgag
      721 cgtgaacggt acgttcatcc acgagcggca ctcgggcgcg acggggttgc ggtagaaccc
      781 ggagccgtcg atggcggcgt agagccgctc ggccttcgcg cggttgcgct cgcccatggc
      841 ggcgagccct cccctggcct tcagccactt gaagacgagg ccggcgaggt accaggaaaa
      901 ggtcggcggc gtgttgaaca tcgagccctc gtccgccacc gaccgatagc gccataccgc
      961 cgggacgtcc ttgcgggcgc ggtccagcag gtcctcgcgc acgatcacga ccacgagccc
     1021 ggaagggccg atgttcttct gcgcgccggc gtagatgagg ccgaagccgc ggacgtcaat
     1081 cgggcgcgac aggatggtgg aggacatgtc ggcgaccagc ggcacgccgc cggtctcggg
     1141 cacgtaaggg aactccactc cgccgatcgt ctcgttcggg cagtagtgga cgtacgcggc
     1201 gccggcggtg agcctgagtg ctgcctgccc tggtgccgtc gtgtagtttg aggcggcctc
     1261 gtcggccgcg acgttcacgc gcttcacgag gcgctgcgcc tcggcgagcg cccgcttcga
     1321 ccagtggccg gtgacgagat agtcgacgcc cgcatccact gctgccgggt tgagcggaac
     1381 caccgaagac tgcgcagccc cgccgccctg cataaagagt acgcggtagt tggcgggtac
     1441 gcgcaggagc tcccgcagat cgctctccgc ctcgcgcgcc attgcgagaa agggcttgcc
     1501 gcggtgactc acctccatca ccgacgcgcc gaggccctgc caatcggtga gctcctcacg
     1561 cgcctgctcg aggacctcga cgggcagcat ggccggcccc gcagcgaaat tgaatgcgcg
     1621 cacggtcacc ctaactaact cggctcgccg ggcgcgggct ccgcacccgc cgcctcgtcc
     1681 tcctcggtgc ccgcctcgag gctctcggtg cgctcgatgc cgaccagacg ctcgccccct
     1741 tcgccgagcc gaatcaggcg cactccctgg gtgttacgtc cctggacgg
//
LOCUS       DATLKL010000135         1938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_57903, whole genome shotgun sequence.
ACCESSION   DATLKL010000135 DATLKL010000000
VERSION     DATLKL010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57903"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VM713_01795"
     CDS             complement(<1..1261)
                     /locus_tag="VM713_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVH83025.1"
                     /translation="MTAANFKLRYAGAAFLTALLAAAAVAALLIVRHQAESSALSTLA
                     EQSSRERVAAELRARAQSVAAHAADSVAGAVRAQDAAGIARRLQPFLEDPTVTAISVD
                     DRLGGTLYSWERATQPAPGAQSAIATVPVRLMMESFGAATPGTLAQLSLTLEQTAPVS
                     AVPLTGELAALRAAHARLSGWLALLVALGGGGAAAALAWRALRELERPVDSIIRSAER
                     IGVGDYSRPLEVRVQDPLAALAQAFERMRGRLRHSTISKNYLQSMLNSMTDAVFITSP
                     DGIIRLANSAAYKLLGFSEEELVNRGIHTILEERERAGFDLLQAAQETRETVVRTRAG
                     QTIPVSFSGSVIASADPQFQGSIFVARNITDRKRAERRIRYLARYDALTKIPNRMQFY
                     HVLQQTIARALRAGQIVAVLYLDMDRFK"
     gene            complement(1335..>1938)
                     /locus_tag="VM713_01800"
     CDS             complement(1335..>1938)
                     /locus_tag="VM713_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83026.1"
                     /translation="VHVSRGARFLEEYLPVLGLGAWVPVAAEVIHFTGYEVPFAQLEA
                     RLADRRDVLLGHLLGTADMIAQIADRCYLEKCRDRLYAEFVLGGVALPFAGGYRQVKY
                     ASGLDLLRQTPDFIEETRRNRLDRDFGAAYRYLEILFGGRNPYIEAIDRNVEFLRQVL
                     RSENWQLLRRRPPIFAAVEDPMGTMRGLMLGYIKRVWANY"
BASE COUNT          302 a          650 c          701 g          285 t
ORIGIN      
        1 ccttgaagcg gtccatgtcg aggtacagca ccgccacgat ctgcccggcg cgcagcgcgc
       61 gcgcgatggt ctgctgcagc acgtggtaga actgcatgcg gttggggatc ttggtgagcg
      121 catcgtagcg ggccagataa cggatgcggc gctcggcgcg cttgcggtcg gtgatgttgc
      181 gcgccacgaa gatgctgccc tggaactgcg ggtcggcgct ggcgatcacc gagccgctga
      241 atgacacggg aatcgtctgg ccggcgcggg tgcgcaccac ggtctcgcgc gtctcctgcg
      301 ccgcctgcag caggtcgaag ccggcgcgct cgcgctcctc gaggatcgta tgaatgccgc
      361 ggtttaccag ctcctcctca ctgaagccga gcagcttgta ggcggccgag ttggcgagcc
      421 tgatgatgcc gtcgggcgag gtgatgaaca ccgcgtccgt catgctgttc agcatgcttt
      481 gcaggtagtt cttgctgatg gtcgagtggc gcagccgccc gcgcatgcgc tcgaaggcct
      541 gcgcgagggc tgcgagcgga tcctgtacgc gcacctcgag cgggcgcgaa tagtcgccga
      601 cgccgatacg ctcggcgctc ctgatgattg agtcgaccgg gcgctcgagc tcgcgcaacg
      661 cgcgccaggc gagcgcggcc gctgcgccgc ccccgccgag ggcaacgagc agcgcgagcc
      721 acccgctgag gcgcgcgtgc gccgcgcgca gcgccgccag ctcacccgtc agcggcacgg
      781 cggacaccgg cgcggtctgc tcgagcgtga gagagagttg cgcgagcgtc ccgggggtcg
      841 ccgctccgaa gctctccatc atgaggcgca ccggcacggt ggcgattgcg ctctgcgctc
      901 cgggggcggg ctgtgtcgcg cgctcccagg aatagagcgt tccgcccagg cggtcgtcga
      961 cggaaatcgc cgtgaccgtg ggatcctcga ggaacggctg caggcggcgg gcgatgccgg
     1021 ccgcgtcctg ggcgcgcacc gccccggcga ccgaatcggc ggcgtgcgcg gcgacgctct
     1081 gcgcgcgcgc gcgtagctcc gcagcgacgc gctcgcgcga ggactgctcg gcgagggtgc
     1141 tcagggcgga ggattccgcc tggtggcgca cgatcaagag cgccgccacc gcggcggcag
     1201 ccagcagcgc ggtaaggaac gcggcacccg cgtagcggag cttgaagttg gcggcggtca
     1261 ctctacggaa tccccggagg tgggggattc atcttactcg ccgcccgggc gcagtcgcta
     1321 ccgggaaacc cgcctcaata gttggcccag actctcttta tatagcccag catcaagccg
     1381 cgcatggtcc ccatggggtc ctcgacggcg gcgaagatgg gcggtcgccg ccgcagcagc
     1441 tgccagttct cgctgcgcag tacttggcgc agaaattcca cattgcggtc gatcgcctcg
     1501 atgtagggat tgcgcccgcc aaagaggatc tcgagataac ggtaggcggc accgaaatca
     1561 cggtcgaggc gattgcggcg cgtctcctcg atgaaatccg gcgtctgtcg cagcaggtcg
     1621 agccccgagg cgtacttcac ctggcggtag ccgccggcga agggcagcgc cacgccgccc
     1681 agcacgaact cggcgtacag gcggtcgcgg cacttctcga ggtaacagcg gtcggcgatc
     1741 tgcgcgatca tgtccgcggt gccgagcagg tggccgagca gcacgtcgcg ccggtccgcg
     1801 agccgcgctt ccagctgcgc gaacggcacc tcgtagccgg tgaagtggat gacctcggcc
     1861 gccacgggca cccaggcgcc gaggccgagc accggcagat actcctcgag gaagcgcgcg
     1921 ccgcgcgaca cgtgcacg
//
LOCUS       DATLKL010000136         1708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_69293, whole genome shotgun sequence.
ACCESSION   DATLKL010000136 DATLKL010000000
VERSION     DATLKL010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69293"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1359
                     /locus_tag="VM713_01805"
     CDS             118..1359
                     /locus_tag="VM713_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HVH83027.1"
                     /translation="MPRGEFKPRPMRLSILTAALQELTPRARRDADPDLAIEEWLEFA
                     REIGAPNIELSAALHPSESDIPAAAMLDPVANTLDLRRPFDAERAARVRRALQATQVG
                     LSDLAYFDNMLAADEAVRSRKHQLMLRVFDAAVLLGTDAVTGFVGRNPLLSMDGNLVM
                     FEAVFVPLLKEAKARGLTFRVEQCPMPGWNVSDAWHNNIAYAPGPWIALHRICERHGV
                     GDQFRIHYDPSHAILMGQDSRSVFQFLKDSGYNFLIGGFHVKGQVIESRGIAAWGYGG
                     QTMQRGDWRGAEPSSDPAEQLNAWKKQTVFAEHELPGTARHDPLAYLQNRSVDWLDHQ
                     LAARELLAIEPEKTYLVVEHEYPPARIQDKVRLKPILAGSLAFTRAIDEAAAAMYALQ
                     HEVLASQGIAVQGIGREPYRS"
     gene            1363..>1708
                     /locus_tag="VM713_01810"
     CDS             1363..>1708
                     /locus_tag="VM713_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HVH83028.1"
                     /translation="MPFVTPKSQQRAYDVIVVGSGAAGGQTAYTLCMEGARVLMLEAG
                     RRYSPESETPMFQTPDMAPLGGDNTPPKPFGFYDATVDGGWEVPGEPYVRTTEDHAGR
                     FDWWRARMLGGRT"
BASE COUNT          271 a          600 c          575 g          262 t
ORIGIN      
        1 acctgtactc ggttggcggc gcgcacgact ggcactccat ctggctgtac cccgcggcgt
       61 tcgccgcggc ggtgttcgcg ctgttcgcgg tcagtttccg caacgagaag gtggcgtatg
      121 ccgcgcggtg agttcaagcc gcggccgatg cgcctgagca ttcttaccgc ggcgctgcag
      181 gagctgacgc cgcgcgcccg gcgcgacgcc gatccggatc tcgccatcga ggagtggctc
      241 gagttcgcac gggagatcgg cgcgccgaat atcgagctgt ccgcggccct gcatccgagc
      301 gagagcgaca tccccgcggc ggccatgctc gaccctgtcg ccaacaccct cgacctgcgc
      361 cggccgttcg atgccgagcg cgccgcgcgc gtacgccgcg cgctgcaggc gacgcaggtg
      421 ggactgtccg accttgccta cttcgacaac atgctggccg cggatgaggc ggtgcgcagc
      481 cgcaagcacc agttgatgct cagggtgttc gacgccgcgg tgctgctcgg caccgatgcc
      541 gtcacgggct tcgtcggccg caatccgctc ctcagcatgg acgggaacct ggtgatgttc
      601 gaggcggtgt tcgtgccgct gctcaaggaa gccaaggccc gcggcctgac cttccgcgtc
      661 gagcagtgcc cgatgcccgg ttggaacgtg agcgacgcct ggcacaacaa catcgcctac
      721 gcgcccggcc cgtggatagc gctgcaccgt atctgcgagc gccacggagt cggcgaccag
      781 ttccgcatcc actacgatcc ctcgcacgcc atcctcatgg gccaggactc gcgctccgtg
      841 ttccagttcc tgaaggacag cggttacaat tttctgatcg gcggctttca cgtcaaaggt
      901 caggtcatcg aatcgagggg tatcgcggcg tggggctacg gcgggcagac gatgcagcgc
      961 ggcgactggc gcggcgcgga gccgagctcg gaccccgccg agcagctcaa tgcctggaag
     1021 aagcagacgg tgttcgccga gcacgagctt cccgggaccg cgcgccacga tccgctcgcg
     1081 tacctgcaga atcgctcggt cgactggctc gaccaccagc tcgccgcgcg cgagctgctc
     1141 gccatcgagc cggagaagac ctatctcgtc gtcgagcacg aatacccgcc ggcacgcatc
     1201 caggacaagg tgcggctgaa gcccatcctg gccggctcgc tcgccttcac gcgcgcgatc
     1261 gatgaggccg cggccgccat gtatgcgctc cagcacgagg tcctcgcgag ccaaggcatc
     1321 gccgtacagg gtatcgggcg cgagccctac cgctcctgag ccatgccgtt cgtgacgccg
     1381 aagtcccagc agcgcgccta cgacgtgatc gtggtcggct cgggcgccgc cggcggacag
     1441 accgcctaca cactgtgcat ggagggagct cgggtactca tgctcgaagc cgggcgccgc
     1501 tactcccccg agagcgagac gccgatgttc cagacgcccg acatggcgcc cttgggcggg
     1561 gacaacacgc cgccgaagcc cttcggcttc tacgacgcca ccgtggacgg cggttgggag
     1621 gtgccgggtg agccctacgt gcgcaccacc gaggaccacg ccgggcgctt cgactggtgg
     1681 cgggcccgca tgctgggcgg gcgcacca
//
LOCUS       DATLKL010000137         2000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_159397, whole genome shotgun sequence.
ACCESSION   DATLKL010000137 DATLKL010000000
VERSION     DATLKL010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159397"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VM713_01815"
     CDS             complement(<1..181)
                     /locus_tag="VM713_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017970965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVH83029.1"
                     /translation="MLVNRAGLVFVGRRIDTTEEAWQMPQGGIDAGETPAEAALRELE
                     EETGTGKAEIIAESRG"
     gene            complement(220..1578)
                     /locus_tag="VM713_01820"
     CDS             complement(220..1578)
                     /locus_tag="VM713_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HVH83030.1"
                     /translation="MMRHVMLATAIAVALMLSPLSGSTQTSKAGNHNSNANSADTYRA
                     LSLFGDVFEKVRQDYVEDVTDDKLVEAAINGMLTSLDPHSGYLNAKSFQDMQIQTRGE
                     FGGLGIEVTLENGLVKVVSPIDDTPAAQAGIKPGDLITHLDGEPVLGMSLEDAVEKMR
                     GPVNSTIRLTVRREKQQPFDVTLKRAVIKIHSVRSQVYDNVGYIRISSFSEQTDEGVK
                     AAVKKLKDSLGKKLAGIVLDLRNNPGGLLDQAVAVSDDFLDHGEIVSTRGRRAEDVQR
                     FNAHGHDLAAGLPLLVLINGGSASASEIVAGALQDHHRAVVLGTRSFGKGSVQTIIPL
                     GNHGAMRLTTARYYTPSGRSIQARGIDPDIVVEQAKVEHVAQTENRSERDLRGALTND
                     TAPDSGSSGNVEPKEQSPSGDKGTGKTDESDKSLDSQDYQLARAIDLLRGLALVSQRA
                     TN"
     gene            complement(1575..>2000)
                     /locus_tag="VM713_01825"
     CDS             complement(1575..>2000)
                     /locus_tag="VM713_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HVH83031.1"
                     /translation="PAIRRMSEAQGRLTLPARGEIVQEFGQSGDYGAASKGVTIRARP
                     GAEVVAPYDGQVVFAGPFRGYGPILIIEHTEGYHTLLAGLSRIDAAPGQWVLAGEPVG
                     VMGEQRDGDGPALYVELRRNGRPIDPLPWLALHSGKVSG"
BASE COUNT          299 a          682 c          656 g          363 t
ORIGIN      
        1 agccgcggct ctcggcgatg atctccgcct tgcctgtgcc ggtctcctcc tcgagctcgc
       61 gaagcgccgc ctcggccgga gtctcgccgg catcgatgcc gccttgcggc atctgccagg
      121 cctcctcggt cgtgtcgatg cggcgcccaa cgaagacgag ccccgcgcgg ttgaccagca
      181 tgatcccgac cccgtcgcgg tacgggcgcg cgtcggaggt cagttagtcg cccgctgact
      241 gaccaacgca agcccgcgca acaggtcgat cgcgcgggcg agttggtagt cctggctgtc
      301 cagcgacttg tccgactcgt ccgtcttgcc ggtccccttg tcgccgctcg ggctctgctc
      361 cttcggctca acgttgccgc tgctccccga atccggcgct gtatcgttgg tgagcgcccc
      421 gcgcaggtcg cgttcgctgc gattctcggt ctgcgccaca tgctcgacct tggcctgctc
      481 gaccacgatg tcggggtcga tgccgcgggc ctggatcgag cggccggagg gggtgtagta
      541 gcgcgcggtg gtgagccgca tggcgccatg gttgccgagc gggatgatgg tctgcaccga
      601 gcccttgccg aaggagcgcg tgccgagaac caccgcgcgg tggtggtcct gaagcgcgcc
      661 ggcgacaatc tcggaggccg aggccgaccc gccattgatc agcaccagca gcggcagccc
      721 cgcggcgaga tcgtgcccgt gggcgttgaa gcgttgcacg tcctcggcgc ggcggccgcg
      781 ggtcgagacg atctcgccat ggtcgaggaa atcgtcggag acggcgaccg cctggtcgag
      841 cagcccgccc gggttgttgc gcaagtcgag gacgatgccg gcgagcttct tgccgagact
      901 gtccttgagc ttcttcaccg ccgccttgac gccctcatcg gtctgctcgc tgaacgagga
      961 gatacggatg tagccgacat tgtcgtagac ctgcgatcgc accgagtgga tcttgatgac
     1021 cgcccgcttc agcgtcacgt cgaagggctg ctgcttctcg cgccggacgg tgagccggat
     1081 ggtggaattg accggcccgc gcatcttctc gacggcatcc tcgagcgaca tgccgagcac
     1141 cggctcgcca tcgagatggg ttatgaggtc gcccggcttg ataccggcct gggccgccgg
     1201 cgtgtcgtcg atcggcgaga ccaccttgac cagtccgttc tccaaggtga cctcgatgcc
     1261 gagcccgccg aattcgccgc gggtctggat ctgcatgtcc tggaagctct tggcattgag
     1321 gtagcccgaa tgcgggtcga gcgaagtgag catgccgttg atcgccgcct cgaccagctt
     1381 gtcgtcggtg acgtcctcga catagtcctg gcgcaccttc tcgaagacgt ccccgaacag
     1441 gctgagcgct cgatacgtgt ccgcgctgtt ggcgttcgag ttgtgattgc cggccttgct
     1501 ggtctgggtc gagccgctca agggcgagag cataagggcg acagcgatcg cggtcgccag
     1561 catgacgtga cgcatcatcc gctcacctta ccactgtgaa gtgccagcca aggcaacgga
     1621 tcgattggcc ggccgttacg acgcaattct acgtaaagcg caggaccgtc gccatcgcgc
     1681 tgctcgccca tcacgccaac cggttcgcct gccagaaccc actggccggg cgccgcgtcg
     1741 atccgcgata gacctgcaag cagcgtatga tagccttcgg tgtgttcgat gatcaagatc
     1801 gggccatagc cgcggaacgg cccggcgaac accacctggc cgtcataggg cgccaccacc
     1861 tcggcccccg gtcgggcccg gatggtcacc cccttgctgg ccgcgccgta atcgccgctt
     1921 tggccgaatt cctgcacgat ctccccgcgc gccgggagcg tcaagcgccc ctgcgcctcg
     1981 ctcatccggc ggatcgccgg
//
LOCUS       DATLKL010000138         2604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_103045, whole genome shotgun sequence.
ACCESSION   DATLKL010000138 DATLKL010000000
VERSION     DATLKL010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103045"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VM713_01830"
     CDS             <1..228
                     /locus_tag="VM713_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH83032.1"
                     /translation="TPNTALFEGQLAMRGGYITVKGGLDGDATATSVPGVFAAGDVAD
                     HVYRQAVTSAGSGCMAALDADRYLETLDARA"
     gene            245..1399
                     /locus_tag="VM713_01835"
     CDS             245..1399
                     /locus_tag="VM713_01835"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HVH83033.1"
                     /translation="MPAARIEFYRSIDQLDPREWNSLTGGAHPFARHEFLAALEHAGC
                     VGPGTGWQPRYLALRDTEGLVGGTAAYLKTHSYGEFVFDFRWAEAYERLGRRYYPKLT
                     VATPFTPATGPRLLVRPGLDQGGIAAQLLEALERDTVAHDVSGVHALFLDEPARAACA
                     ARGWLMRRDCQFHWSNRGYASFEDYLETFTAEKRKKARRERRRVAESGVRFETRFGAE
                     LDAPLLERIYGLHRDTFLRHGHEPYLTAAFFAEVAQRLPESLMVKLAIHGRTVVAVAI
                     FFASADALYGRYWGAAADFHSLHFEACYHQGVEFCIERGIARFEPGTQGEHKVSRGFE
                     PQLTWSAHFIADRQFRAAIAQYLEREGAAVESYSLEVQEHVPYRGRRTAR"
     gene            1406..2092
                     /gene="aat"
                     /locus_tag="VM713_01840"
     CDS             1406..2092
                     /gene="aat"
                     /locus_tag="VM713_01840"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HVH83034.1"
                     /translation="MKTVTWLSAHDAPEWFPPPEQALEDPPGLLAAGGDLSPERLLAA
                     YRRGIFPWYSPGQPVLWWSPDPRAVLFPEEFHRTRSLAKTLRNGGFSTSIDRDFAAVI
                     DGCTAPRTASPGTWITSDMRTAYLKLHRLEWAHSIEVWRDGVLAGGLYGVRIGGVFFG
                     ESMFSVVRDGSKVALAHLVALCPRNSIAVIDCQLYSRHLGSLGARTIPRAVFQRLLRE
                     QVALPSPPLA"
     gene            2167..2385
                     /gene="infA"
                     /locus_tag="VM713_01845"
     CDS             2167..2385
                     /gene="infA"
                     /locus_tag="VM713_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HVH83035.1"
                     /translation="MSKEDAIQMEGEVVETLPNTTFRVKLKNGHVVTAHISGKMRKNY
                     IRILTGDAVTVEMTPYDLTKGRIIYRGR"
     gene            complement(2405..>2604)
                     /locus_tag="VM713_01850"
     CDS             complement(2405..>2604)
                     /locus_tag="VM713_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83036.1"
                     /translation="ERGYDPKMGARPMARTIQEHIKRPLAEELLFGRLSSGGHVRVSV
                     SEDGSQLILHCEPARVPEVTA"
BASE COUNT          400 a          934 c          845 g          425 t
ORIGIN      
        1 acgcccaaca cggcgctctt cgaggggcag ctcgccatgc gcggcggcta catcaccgtg
       61 aagggcggtc tcgacggcga tgcgactgcg accagcgttc ccggggtatt cgctgccggc
      121 gacgtggcgg atcacgtcta ccgccaggcg gtgacttccg ccggctccgg ctgcatggcc
      181 gcgctcgacg ccgaccgcta cctcgagacg ctcgacgcac gcgcgtagcg gctccgaccc
      241 caccatgccc gccgcccgga tcgagttcta ccgcagcatc gatcagctcg acccgcggga
      301 gtggaattcg ctgaccggag gtgcgcatcc gttcgcacgc catgaatttc tcgcagctct
      361 cgagcacgcc gggtgcgtag gtcccggcac cggttggcaa ccgcgctacc tggcgctgcg
      421 cgataccgag ggtctcgtcg gcggcaccgc cgcctatctc aagacacact cctatgggga
      481 gttcgtcttc gacttccgct gggcggaggc ctatgagcgg ctcggccggc gctactaccc
      541 taagctcacc gtagccactc cgttcacgcc cgcgaccggc ccgcgcctgc tggtgcgccc
      601 ggggctcgat cagggaggca tcgccgcgca gctcctcgag gcgctcgagc gcgacaccgt
      661 ggcccacgac gtctcgggcg tacatgcgct gttcctcgat gagcccgcac gcgcagcctg
      721 cgccgcgcgt ggctggctca tgcgccgcga ctgccagttc cactggagca atcgcggcta
      781 cgcgagcttc gaggactacc tcgagacctt taccgccgag aagcgcaaga aggcgcggcg
      841 cgagcgccgg cgcgtggccg agagcggcgt gcgcttcgag acgcgcttcg gcgccgagct
      901 cgacgctccg ctcctcgagc gcatctacgg cctgcaccgc gacaccttcc tgcgccacgg
      961 gcacgagccg tatctgacag ccgcattttt cgccgaggtg gcgcagcgac tgcccgagtc
     1021 gctgatggtg aagctcgcca tccacgggcg caccgtagtc gcggtggcga tcttcttcgc
     1081 gagcgcggac gcgctctacg gccgctactg gggcgccgcg gctgacttcc acagcctgca
     1141 cttcgaggcc tgttatcacc agggggtcga gttctgcatc gagcgcggca tcgcgcgctt
     1201 cgagccgggt acgcagggcg agcacaaggt gagccgcggc ttcgagccgc agctcacctg
     1261 gtcggcgcac ttcatcgccg accggcagtt ccgcgccgcc atcgcccaat acctggagcg
     1321 cgaaggcgcg gcggtggaga gctactcact cgaggtccag gagcacgtcc cgtaccgcgg
     1381 acggcgcacc gcgcggtaag cgcgcatgaa aaccgtcacc tggctctcgg cgcacgatgc
     1441 acccgagtgg tttccgccac ccgagcaggc gctcgaggac ccacccgggc tgctcgccgc
     1501 cgggggcgac ctctcgcccg agcggctgct cgccgcctat cggcgcggca tattcccctg
     1561 gtactccccc ggtcagccgg tgctgtggtg gtcgcccgat ccgcgcgcgg tgctctttcc
     1621 ggaggagttt caccgcacgc gcagcctcgc caagacgctg cgtaatggcg ggttcagcac
     1681 ctcgatcgat cgtgacttcg ccgcggtgat cgacggctgc accgcgccgc gcactgcgag
     1741 ccccggcacg tggatcacct ccgacatgcg caccgcgtac ctgaagctgc accgcctgga
     1801 atgggcccac agcatcgaag tctggcgcga cggcgtactc gccggcggac tctacggcgt
     1861 gcgcatcggc ggcgtattct tcggggaatc gatgttcagc gttgtgcgcg acggctccaa
     1921 ggtcgcgctc gcccatctgg tggcgctgtg tccgcgcaac agtatcgcgg tgatcgattg
     1981 ccagctctac tcgcgccacc tcggcagtct tggcgcccgc accatcccgc gcgccgtatt
     2041 ccagcggctg ctgcgcgagc aagtggcgtt gccgtcaccg ccgctcgcgt agcaggcggc
     2101 gcgggcaaat tgcctttgcg gcggcctttg tgctccaatt cgcgccccgc agtcgttaag
     2161 ggcaccatgt ccaaagaaga cgcgattcag atggaaggcg aggtcgtcga gaccctgccc
     2221 aacacgacct tccgggtgaa gctgaagaac ggccacgtcg tgaccgcgca catctcgggg
     2281 aagatgcgca aaaactacat ccgcatcctg accggtgatg cggtgacggt ggagatgact
     2341 ccctacgatc tcacgaaggg ccgcatcatt taccgcggcc ggtaggccaa ggtacttcga
     2401 ccgatcaggc ggtgacttcc ggcacgcgcg ccggctcgca gtgtaggatt agctgcgatc
     2461 catcctcgct gaccgacacg cgcacgtgcc cgccgctcga gagacgtcca aacagcagct
     2521 cctcggcgag cggccgtttg atgtgctcct ggatcgtgcg cgccatgggg cgcgccccca
     2581 tcttcgggtc gtagcccctc tcgg
//
LOCUS       DATLKL010000139         1682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_170715, whole genome shotgun sequence.
ACCESSION   DATLKL010000139 DATLKL010000000
VERSION     DATLKL010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170715"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VM713_01855"
     CDS             <1..700
                     /locus_tag="VM713_01855"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HVH83037.1"
                     /translation="PEQLMNKTQPCAALLALAFTAAGGFAAERNTASLTWRPLLEAHA
                     ARDWRGWKEAGLPAGWHVDGGVLHKDGPVDDLVTNQKFGNFVLELEWKIGKAGNSGVF
                     YRGTREYDHIYWSAPEYQLLDDANTEDGKGPLTAAGSAYGLYGVPPGVAKPYGQWNRT
                     RLVVRGNHVEHWLNDVKVVEYEFASDDWKKRVAGSKFSQYPNYGLAPSGLIGIQGDHP
                     GALAIRRIRIRELP"
     gene            727..>1682
                     /locus_tag="VM713_01860"
     CDS             727..>1682
                     /locus_tag="VM713_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH83038.1"
                     /translation="MSVQLAESMRRISGLLQAGEYGAAHAQLQSLVAANPNYLEGLRL
                     LAGTKQALGDTAQAESLLRRALEIDPASPPTLTTLAELLLVSGRRAEAVPLLQRAVQG
                     APPYPRAALILVRHYLEAGQPALALEAAAAWCNSGQADMDLAALHVAAYAALGRQGEA
                     VAHYRRLVARAPDNPVATHTLAVALHAAHQPEEAERVARQTLKRTQPTAALHHTHARS
                     LLSLERFEEAEVALHECVRLDPRRAEAHDRLAELIWMRTGDILEATRSLDRALEKLPH
                     DDALRATKAALLQGAGDARGAFACLAERAVQPQSHPSLLIRAG"
BASE COUNT          284 a          582 c          563 g          253 t
ORIGIN      
        1 accggagcaa ctcatgaata agactcagcc ctgcgcggcg ctgctggcgc tcgccttcac
       61 ggccgccggc ggcttcgccg cggaacggaa caccgcctcc ctcacctggc gcccgctgct
      121 cgaggctcat gccgcccgcg attggcgggg ctggaaggag gccggcttgc ccgcgggctg
      181 gcatgtcgat ggcggggtgc ttcacaagga cggtccggtg gacgatctcg tgacgaacca
      241 gaagttcggc aacttcgtgc tcgagctcga gtggaagatc ggcaaagcgg gtaacagcgg
      301 cgtcttctac cgcggcacgc gcgagtacga ccacatctac tggagcgcgc cggaatacca
      361 gctgctcgat gatgccaaca cggaggacgg caagggccca ctgaccgccg ccggctccgc
      421 ctatgggctc tacggcgtgc cgcccggtgt tgcgaagccc tatggtcagt ggaaccggac
      481 ccgcctcgtc gtgcgcggca accacgtcga gcactggctg aacgacgtca aggtggtcga
      541 gtacgagttt gcgagcgacg actggaaaaa acgggtggca gggagcaagt tctcccaata
      601 cccgaactac ggactcgcgc cgagcgggct catcggcatt cagggcgatc accccggggc
      661 gctcgcaatc cggcgcatca ggatacgcga gctcccctga caggatcagc gtcgccgagg
      721 tcttccgtgt cggtacagct cgccgagtcc atgcgccgca tcagcggcct gctgcaagcg
      781 ggcgagtacg gcgcagcgca cgcgcagctg caatcactgg tagccgcaaa tccgaattac
      841 cttgaagggc tgcgcctgct ggccggcacc aagcaggcac tgggcgacac agcgcaggcc
      901 gaatctctgc tgcggcgggc cctcgagatc gaccccgcgt cccctcccac gctgaccacc
      961 ctggcagagc tactgctcgt ctcgggccgc agggccgagg cagtacctct gttgcagcga
     1021 gccgttcagg gcgcccctcc ttacccgcgg gcagcgctca ttctggtgcg ccactatctg
     1081 gaggcgggtc agccggcact ggcgctcgag gctgccgcgg cctggtgcaa cagcggtcag
     1141 gctgacatgg acctggcggc gttgcacgtt gcggcctatg ccgctctggg tcggcagggc
     1201 gaggcggtgg cgcactatcg tcgtctggta gcacgcgctc cggacaaccc ggtcgcaacc
     1261 cataccctgg cggtcgcgtt gcatgcggcc catcagccgg aggaagccga gcgagtcgcg
     1321 cgtcagacgc tcaagcgcac tcaaccgacg gcggcgctgc accacacgca cgcccgcagc
     1381 ctcctgagcc tcgagcggtt cgaggaagcc gaggtcgcgc tgcacgagtg cgtgagactt
     1441 gatccgcgcc gcgccgaggc ccacgaccgg ctggcggagc tcatctggat gcgcaccggc
     1501 gacatcctcg aggccacgcg ctctctggat cgggcgctcg aaaagctccc gcacgacgat
     1561 gcgctgcggg ccaccaaggc ggccttattg cagggcgccg gggatgcgcg cggcgctttt
     1621 gcgtgccttg ctgaacgtgc cgtgcaaccc caatcgcacc ccagcctcct cattcgcgcg
     1681 gg
//
LOCUS       DATLKL010000140         1733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_46624, whole genome shotgun sequence.
ACCESSION   DATLKL010000140 DATLKL010000000
VERSION     DATLKL010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46624"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            686..889
                     /locus_tag="VM713_01865"
     CDS             686..889
                     /locus_tag="VM713_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83039.1"
                     /translation="MTNTQDLLAYLATDAGKWAAEFCKSARDKGLRYDEDLMRGWFAQ
                     AIMAGRIDEAKMWARLKQQETCS"
     gene            886..1146
                     /locus_tag="VM713_01870"
     CDS             886..1146
                     /locus_tag="VM713_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83040.1"
                     /translation="MSDTQGTLPIGAPAGEMAPISPVVPPFTPPPGSAITGLNEAKAA
                     AAANGVAAVMFLAEVFTNEEFDITERVQAAAELLQFLAKGPF"
     gene            1146..1718
                     /locus_tag="VM713_01875"
     CDS             1146..1718
                     /locus_tag="VM713_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83041.1"
                     /translation="MPVTLTDEQVAELRARLGQAETNAKIAEATARIWNDPERGERAK
                     ALWKEQYPDTDLGPYDVERRINERLDKERLERQQAEKAAREKEQDERLAKKRSETKDR
                     YGATDDAMERLERMMVERNIGDYDVAAEHFFTREPRVSDGTQEYDSQFWQHEKGDLFK
                     EISADPEAWGRKEILKTLRDQEQNQRGGWR"
BASE COUNT          372 a          565 c          566 g          230 t
ORIGIN      
        1 ggcccaccag cagcccccgg cggccccccg ggagccggcg gtgcatcgcc tattctcgct
       61 gctctggctc gcgcacgcgg tgcgccgcaa gcctcggccc ccggccaggg cgcacaggcc
      121 gccgcgctgc aatcagtaaa aatggctttg gatatgttgc aagccgcgct gcctagcctc
      181 ggtaccggca gcgaagttca ccgcgacgtg atcaacgcgg tgagccgcct cagccgccac
      241 atgccgcagg gcatcccgac cgccggcgcg cagcagggcg cagtgcagga tctactgcga
      301 aatactgtaa gaaatgcaat gcttcagcgc gttatggcca accaaggtgg tggcgggcag
      361 cagccgatgc cgccgtccac cccacttcca ggcgcttagc ctagccatgc caagcctgcc
      421 ccgacccgcc aggccacgcc atgcccgtcc cagccgtgcc gagccacgcc ggggcggatt
      481 atacaggaga taaaccattg gcacaaaaca gaagttacga ccctccgatc agcaccccgc
      541 ccgacgtgcc acctcgtact attagacaag ttgacacgca gagcgagaca agcgagtggg
      601 gagcaatacc aaaagttgtt ccgcggcccg ctggcggcat tccgctgcag ccgagcatta
      661 ccgggcgcag cgacgggccg acgcgatgac gaacacgcag gacttgctgg cctatctggc
      721 aaccgatgcc ggtaagtggg cggccgagtt ctgcaaaagc gcgcgcgaca aggggttgcg
      781 ctacgacgag gacttgatgc gcggctggtt cgcccaggcg atcatggccg ggcgcatcga
      841 tgaagcgaag atgtgggcgc ggctcaagca gcaggagacg tgctcgtgag cgacacgcag
      901 ggcacgctgc cgatcggtgc cccagccggc gaaatggcgc cgatctcgcc ggtcgtgccg
      961 ccgtttaccc cgccgccggg cagcgcgatc accggactca acgaggccaa ggccgccgca
     1021 gcggccaatg gcgtagcagc cgtgatgttc ctcgccgagg tcttcaccaa cgaggaattc
     1081 gacatcaccg agcgcgtgca ggccgccgcc gagttgctgc agttcctggc gaaagggcca
     1141 ttctgatgcc ggtcaccctc accgacgaac aggtcgccga gctccgcgca aggttgggcc
     1201 aggctgagac gaatgcgaaa atagctgaag ccacggccag gatttggaac gatcccgagc
     1261 gcggcgagcg cgccaaggcg ctgtggaaag agcagtaccc cgacaccgat ctcggcccct
     1321 acgatgtcga gcgccggatc aacgagcgcc tcgacaagga gcggctggaa cgtcagcagg
     1381 ccgagaaggc cgcgcgcgag aaggagcagg acgagcgcct cgccaagaag cgatcggaaa
     1441 ccaaagaccg ctacggcgcg accgacgacg cgatggagcg gctcgaacgc atgatggtcg
     1501 agcgcaacat tggcgactac gacgtggccg ccgagcactt cttcacccgc gagccgcgcg
     1561 tcagcgacgg tacgcaggag tacgacagcc agttctggca gcatgaaaaa ggcgatctct
     1621 tcaaggagat ttccgccgac ccggaagctt gggggcggaa agagattctc aagacgctaa
     1681 gggatcaaga gcagaatcag cgggggggat ggcgctagcg ccaaacctgg gat
//
LOCUS       DATLKL010000141         2459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_125647, whole genome shotgun sequence.
ACCESSION   DATLKL010000141 DATLKL010000000
VERSION     DATLKL010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125647"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VM713_01880"
     CDS             <1..220
                     /locus_tag="VM713_01880"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH83042.1"
                     /translation="DYDSAAEAFRAVLKKWPGAHKAPDALLKLGFTQYEQKQYGAARS
                     TLTTVTQKYPGTDAAKLATDKLRRFPAQ"
     gene            257..904
                     /gene="queE"
                     /locus_tag="VM713_01885"
     CDS             257..904
                     /gene="queE"
                     /locus_tag="VM713_01885"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016711990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HVH83043.1"
                     /translation="MSASRLKITEIFHSLQGEADTVGFPTVFVRLTGCPLRCRYCDTE
                     YAFYGGEWWELERIVARVQEFSTPYVCVTGGEPLAQKACLDLLTQLCDAGLRVSLETS
                     GAMPLGGVDPRVVRVVDVKTPGSGEEQRNRYAELAGLRPPDLVKFVICGREDYEWSRA
                     KLRELTLPAGCTVLFSPSHEQLPGRELADWILEDRLPVRFQIQLHKYLWGNVPGK"
     gene            926..1612
                     /gene="queC"
                     /locus_tag="VM713_01890"
                     /pseudo
     CDS             926..1612
                     /gene="queC"
                     /locus_tag="VM713_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005399706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
     gene            1682..1754
                     /locus_tag="VM713_01895"
     tRNA            1682..1754
                     /locus_tag="VM713_01895"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1715..1717,aa:Lys,seq:ttt)
     gene            1853..>2459
                     /locus_tag="VM713_01900"
     CDS             1853..>2459
                     /locus_tag="VM713_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83044.1"
                     /translation="MASIQRVVSPLTGEVTYRAQVRRKGRSAESATFANRKEAQAWAG
                     SIEAAIRENRHFPNAVAKRTSFDTLVEDYTRTVLKEPGRFDEKERTTRERQLKWWSEK
                     FAGLSLAEVTADRISAARDALSEEVFARGKAHENPKTGEVVEPERYKRSGATINRYLA
                     ALSHALSFAVKDRRVLDRNPVSDIRRKKEPRGRTRFLSDEER"
BASE COUNT          423 a          792 c          835 g          409 t
ORIGIN      
        1 cgactacgac tccgcggccg aagcgtttcg cgccgtgctg aagaagtggc ccggcgcgca
       61 caaggccccc gacgcgctgc tgaagctcgg tttcacgcag tacgagcaga agcagtacgg
      121 tgccgcgcgc tccaccctga cgacggtgac gcagaagtat cctggaaccg acgcggccaa
      181 actggctacc gacaagctgc ggcgcttccc ggcgcagtaa cggcggcgcg cttgggcggc
      241 gccgagcaaa cgcgacatga gcgcctcgcg cctcaagatc accgagattt tccactccct
      301 gcagggtgag gccgacaccg tgggcttccc gacggtattc gtgcgcctga ccggctgccc
      361 gctgcgctgc cgatactgcg acaccgagta cgcgttctac ggcggcgaat ggtgggagct
      421 cgagcgcatc gtggcgcgtg tacaggaatt ctcgaccccc tatgtttgtg tcaccggcgg
      481 tgagcccctc gcgcagaagg cctgcctgga tctgctcacg cagctgtgcg acgcggggct
      541 gcgggtctcg ctcgagacga gcggggcaat gccgctgggc ggagtcgatc cgcgtgtggt
      601 gcgggtggtc gacgtcaaga cgccgggctc gggcgaggag cagcgcaacc gctacgcgga
      661 gctcgctggc ctacggccgc cggacctcgt aaagttcgtc atctgtggcc gcgaggacta
      721 cgagtggagc cgcgcgaagc tgcgcgagct cacgctgccc gccggctgca ccgtgctgtt
      781 ctcgccgagc cacgagcagc taccggggcg ggagctcgcg gactggattc tcgaggatcg
      841 cctgccggtg cgcttccaga tccagctgca caagtacctc tggggcaatg tgccggggaa
      901 ataacatgcg cgcgccgcgc ggtcagtctt cgtgagcgca tcgcccgcgg tggtgctcct
      961 gtccggcggg cttgactccg ccacggcgct agccatcgcc cgcgaccggg gtttcgcctg
     1021 ccacgcgctg tcggtcgact atggacagag gcacgccgcc gagctcgggg cggcgcgcag
     1081 agtcgcgcgc cgcggccagg cacaggagca ccgcatcatg cgcgtcgacc tggcggggat
     1141 tggcggctcg gcgctcacgg atcccacgat cgcggtgcct gaggagccga ccagtgggat
     1201 cccggtcacc tatgtgccgg cgcgtaacac catcatgctg gcgctggcgc tcgggtgggc
     1261 cgaggtgctc ggtgcgcgcg agattttcgt cggggtgaac gttctcgact cgagcggata
     1321 tcccgactgc cgccccgaat acatcgccgc cttcggcgag ctcgcgcggc tcgcgaccaa
     1381 ggccggagtc gagggaaatc cgtgtcgcat cgaggcgccg ctcatcggcc tgaccaaggc
     1441 cgagatcatc cgcgaaggca cgcggctcgg cgtcgactac gccgagaccg tgtcctgcta
     1501 ccaggcggac gacgcgggcc gcgcctgcgg gcgctgcgac gcctgccgcc tgcgtcgcgc
     1561 agggttcgcc gcggcgggat tgtccgatcc gacccgctac cagtccctgt agtatcatgc
     1621 ggcccccgcg cgggcggtcg cttacggctg tttgccgcag cgcgcacgtg gattcgtcgt
     1681 cggggcgtta gctcagtcgg tagagcatcg ggcttttaat ccgctggtcg acggttcgac
     1741 tccgtcacgc cccatataaa tcaactagtt acgatcaatg tgaggcactg ctttgcactc
     1801 ggtgcaaatt cagtgcaaat ttgagtcatt cggcgcccca gggatactcg gaatggcttc
     1861 aattcaacgc gtcgtttcac cgctgacggg tgaggtcact taccgcgctc aagtgcgccg
     1921 caaagggcgg tccgcggaat ccgcaacgtt cgcgaatcgc aaggaagcgc aggcatgggc
     1981 cggttctatc gaggcggcca tccgagagaa ccgacacttc cctaatgcag tggctaagcg
     2041 cacgagcttc gacacgctcg tggaggatta cacgaggacc gtgttgaaag agcctggtcg
     2101 cttcgacgag aaggagcgga cgacgcgcga acggcagctc aagtggtggt cagagaagtt
     2161 cgccgggctt tcgcttgccg aggtgacggc cgaccggatc tccgcggcac gcgacgcact
     2221 ttcagaggaa gtgttcgcgc gcggtaaggc acatgagaat cccaagaccg gcgaggttgt
     2281 ggaaccggag cgatataaac gctcgggcgc cacaatcaac cgataccttg cggcgctctc
     2341 gcatgcactt agcttcgcgg ttaaggatcg gagagtgctc gaccgcaacc cggtcagcga
     2401 cattcggcgt aaaaaagagc cccgtgggcg gacccgcttt ctctccgatg aggagcgcg
//
LOCUS       DATLKL010000142         2231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_35514, whole genome shotgun sequence.
ACCESSION   DATLKL010000142 DATLKL010000000
VERSION     DATLKL010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35514"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VM713_01905"
     CDS             complement(<1..333)
                     /locus_tag="VM713_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVH83045.1"
                     /translation="MNICVLGGTGFVGTELVTRLALAGHWVRVPTRNLTRAQRLRVLD
                     TVGLRVANVHAPAALGQLFADCDAVINLIGILNPRRGASFQTVHAELPAKIVAAARGA
                     GVRHVLHMS"
     gene            complement(345..1433)
                     /locus_tag="VM713_01910"
     CDS             complement(345..1433)
                     /locus_tag="VM713_01910"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HVH83046.1"
                     /translation="MKAAVLALGLSVLAFVPPAHPGVPPAVGDTPLPSLAPMIRKVSP
                     AVVNVATRGTMRDSGAQNPLLQDPFFRRFFEVPRGRAPRERLFRSAGSGVIFDARQGY
                     IVTNAHVVENATEITVTLQDGRDLKAEVVGSDKDSDVAVLKVSADGLAQMPLGDSSHL
                     EVGDFVVAIGNPFGLQHTVTSGIVSGLSRTGINPDGYEDFIQTDASINPGNSGGALVN
                     LRGELIGINTEIIARNGGNVGIGFAIPVNMARSVVDQLIKYGAVKRGQLGVSMYTVTP
                     DIAHSLGLASAVGALVSQVVEGSPADKAGLRVGDVITSVNGQAVKSNSELRNAIGLMR
                     VGDSVDIGLVRDSKPMRLTAVIAAAAGH"
     gene            complement(1543..1962)
                     /locus_tag="VM713_01915"
     CDS             complement(1543..1962)
                     /locus_tag="VM713_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HVH83047.1"
                     /translation="MTIVRYEPWALFGRLNRQLDQAFGDTNASVSWIPHVDVREEAER
                     FVVTADLPGVEGKDIEITADKGVLTVRGERRSEKKDSGEGYERVERASGTFLRRFTLP
                     ESADAEAIKATHVNGVLELSIPKRPQAQPRRINVQAA"
BASE COUNT          345 a          746 c          784 g          356 t
ORIGIN      
        1 gctcatgtgt agcacgtggc gcaccccggc gccgcgtgcc gccgcgacga tcttggcggg
       61 gagctcggcg tggaccgtct gaaagctcgc gccgcgccgc gggttgagaa tgccgatgag
      121 attgatcacg gcgtcacagt cggcgaacag ctggccgagc gccgccggcg cgtgcacgtt
      181 ggccacgcgc agcccgacgg tatcgagcac ccgcagacgc tgcgcccgcg tcaggttgcg
      241 ggtcggcacc cgcacccagt gccccgcgag cgcgaggcgc gtgacgagct cggtccccac
      301 gaagccggtt ccgccgagca cgcagatgtt cacggcgtcc tccttcagtg tccggcggcc
      361 gcggcaatca ccgcggtcag ccgcatgggc ttgctgtcgc gcaccagtcc gatgtccacg
      421 ctgtcgccga cgcgcatcag cccgatcgcg ttgcgcagct cgctgttgga ttttaccgcc
      481 tgcccgttca ccgaggtgat cacgtccccg acgcgcagcc ctgccttgtc ggccggcgag
      541 ccctcgacca cctgcgatac cagcgcgcca accgcgctcg cgagcccgag cgaatgggcg
      601 atgtcgggtg tcaccgtgta catggagacc ccgagctggc cgcgcttgac cgcgccgtac
      661 ttgatgagct gatccacgac gctgcgcgcc atgttgaccg gaatggcgaa gccgatgccg
      721 acgttgccgc cgttacgggc gatgatctcg gtgttgatgc cgatcagctc gccgcgcaga
      781 ttcacgagcg cgccgccgga attgcccgga ttgatcgagg cgtccgtctg gatgaaatcc
      841 tcatagccgt cgggattgat gccggtgcgc gacagcccgc tgacgattcc cgaagtgacg
      901 gtgtgctgca ggccgaaggg gttgccgatg gccacgacga aatcgccgac ctcgaggtgc
      961 gaggaatcgc cgagcggcat ctgcgcgagc ccgtcggcgc tcaccttgag cacggcgacg
     1021 tccgagtcct tgtcgctgcc gaccacctcg gccttgaggt cgcggccgtc ctgcagcgtc
     1081 acggtgatct cggtggcgtt ctcgacgacg tgggcgttgg tgacgatgta gccctgccgg
     1141 gcgtcgaaga tgacgcccga gccggcgctg cggaacaggc gctcgcgcgg ggcccggccg
     1201 cgcggcacct cgaaaaagcg gcgaaagaac gggtcctgga gtagcgggtt ctgcgcgccg
     1261 ctgtcgcgca tggtgccgcg ggtcgcgacg ttcaccaccg cgggcgagac tttgcggatc
     1321 atcggcgcaa gcgacggcag cggcgtgtcg ccgaccgccg gaggaacgcc agggtgagcg
     1381 ggcgggacga acgcgagcac gctcaagccg agggcgagaa ccgcggcttt catatgggca
     1441 ggaattctag cctgcgccgt gaggcggggc ttcagccgcg aaaaaagctc acgccggcgg
     1501 cgccacgcgg gcgccgccgg acgagctttg gggaggctta agtcaggcgg cctggacgtt
     1561 gatgcgccgc ggctgtgcct gcgggcgctt cggaatgctc agctcgagca ccccgttgac
     1621 gtgcgtcgcc ttgatggcct cggcgtcggc actctcgggg agcgtgaagc ggcgcaggaa
     1681 agtgccgctc gcgcgctcga cgcgctcgta gccctcgcct gagtccttct tctccgagcg
     1741 gcgctcgccg cggaccgtga gcacgccctt gtcggccgtg atctcgatgt ccttaccctc
     1801 gacgcccggc aggtcggcgg tgaccacgaa gcgctcggcc tcttcgcgca cgtcgacgtg
     1861 cgggatccag ctgacgctgg cattggtatc gccgaaggcc tgatcgagct gacggttcag
     1921 ccgcccgaag agcgcccacg gctcgtaacg aacgatagtc attgaaagat ctcctcttgg
     1981 aactgaagtt gaaaaagcgc tgcgcaattg cgcttagcct cgatgtgcgg atgctcgccg
     2041 gacgtttcaa gagtgacgcc agcgctttgc ggcgcgcggc gcgcactgcg ccgaaggctg
     2101 cgcgatttcg caggcggaga aagcatcgac agcactcagg gcttggccgc agcggcgctc
     2161 gccggatgca tcggcgccga tcggccgtcc gcaggtcgcg ctgggcaccg ctgctcgggc
     2221 gagactgctg g
//
LOCUS       DATLKL010000143         2086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_159490, whole genome shotgun sequence.
ACCESSION   DATLKL010000143 DATLKL010000000
VERSION     DATLKL010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159490"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VM713_01920"
     CDS             <1..842
                     /locus_tag="VM713_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HVH83048.1"
                     /translation="NAFELMKQMIDAGAAGVHFEDQLSSAKKCGHMGGKVLVPTQEAI
                     AKLVAARLAADVCNVPTILVARTDAEGATLLTSDVDERDRPFIDPTRGRTAEGFFTVK
                     AGLPAAIARGCAYAPYVDLVWCETAKPDLEEAREFAAGIHAQFPGKLLAYNCSPSFNW
                     RKKLDDGEIARFQRELAAIGYRFQFITLAGFHALNYSMFELARAYKAQQMTAYVALQQ
                     AEFAAESAGYTATKHQREVGAGYFDAVTQVVSGGRSSITALAGSTEEQQFASAEPALE
                     KAV"
     gene            complement(859..>2086)
                     /locus_tag="VM713_01925"
     CDS             complement(859..>2086)
                     /locus_tag="VM713_01925"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HVH83049.1"
                     /translation="DAQGEVSRGIEIVEFACGIPQLLNGGFTDQVSTGIDNWTLRQPL
                     GVVAGITPFNFPCMVPCWMFPLAIACGNSFVLKPSERDPSAAVVMAQLLGEAGLPPGV
                     FNVLQGDREAVEALLADPDVKAVSFVGSSPIAHSIYTRGASSGKRVQALGGAKNHMVV
                     MPDADLNQAVDALIGAAYGSAGERCMAISVAVLVGDVADRVIPALAERARGLRVGDGM
                     GRQAEMGPVINREALERIRGYIAAGAAAGAELVVDGRIDAASGRPFTIAGQERGFWIG
                     PTLFDRVTREMRIYQEEIFGPVLACVRVPDFAAAVALVNSHPLANGVACYTRDGHVAR
                     EFGRRIQVGMVGINVPIPVPMAWHGFGGWKGSMFGDTHAYGEEGVRFYTRQKSIMERW
                     PEATPKGPEFTMPTAK"
BASE COUNT          341 a          740 c          697 g          308 t
ORIGIN      
        1 tgaatgcctt cgagctcatg aagcagatga tcgacgcggg agccgccggc gtgcatttcg
       61 aggatcagct gtcttccgcc aagaaatgcg gccacatggg tggcaaggtg ctggtgccca
      121 cccaggaggc gatcgcgaag ctcgtcgctg cgcgcctcgc ggccgacgtc tgcaacgtgc
      181 cgaccatcct cgtggcgcgc accgatgccg agggtgcgac gctcctcact tcggatgtcg
      241 atgagcgcga tcgacccttc atcgacccga cccgcgggcg caccgccgag ggcttcttca
      301 ccgtgaaggc gggcctgccc gcggcgatcg cgcgcggctg cgcctacgcg ccgtacgtgg
      361 acctcgtatg gtgcgagacc gccaagcccg atctcgagga ggcacgggag ttcgccgcag
      421 gcatccacgc gcagttcccc ggcaagcttc tcgcctataa ctgttcaccg tccttcaact
      481 ggaggaagaa gctcgatgac ggggagatcg cgcgcttcca gcgcgagctc gcggccatcg
      541 gctatcgctt tcagttcatc acgctggccg ggttccacgc gctcaactac tcgatgttcg
      601 agctcgcgcg cgcctacaag gcgcaacaga tgaccgcgta cgtggccctg cagcaggcgg
      661 agttcgccgc cgagagcgcc ggctacaccg cgaccaagca ccagcgcgag gtcggcgccg
      721 ggtatttcga tgcggtcacc caggtcgtga gcggcgggcg gtcctcgatc acggcgctcg
      781 ccggcagcac cgaggagcag cagttcgcca gcgcggaacc ggctctcgag aaggcggtat
      841 agagcggcgc gggcggagtt atttcgccgt cggcatggtg aattcggggc ccttgggcgt
      901 cgcttccggc cagcgctcca tgatggactt ctgccgcgtg tagaagcgca cgccctcctc
      961 gccgtaggca tgggtgtcgc cgaacatgct gcccttccac ccaccgaacc cgtgccaggc
     1021 cattggcacc gggatcggca cgttgatgcc caccatgccg acctggatgc ggcgcccgaa
     1081 ctcgcgcgcc acgtgcccgt cgcgggtgta gcaggcaacg ccgttggcga gcgggtgcga
     1141 attgaccagc gcgaccgccg cggcgaagtc cggcacgcgc acgcacgcga gcaccggtcc
     1201 gaagatctcc tcctggtaga tgcgcatctc gcgcgtcacg cggtcgaaga gcgtcggccc
     1261 gatccagaag ccgcgctcct ggccggcgat ggtaaagggg cggccgctcg cggcatcgat
     1321 gcggccatcg acgacgagct cagcgccggc cgcggcacct gcggcgatgt agccgcggat
     1381 gcgctcgagc gcttcgcggt tgatcaccgg ccccatctcc gcctggcggc ccatgccgtc
     1441 gccgacccgc agcccacgcg cgcgctcggc gagcgccggg atcacgcgat cggccacgtc
     1501 gccgaccagc accgccaccg agatcgccat gcagcgctcg ccggccgagc cgtaggccgc
     1561 gccgatcagc gcatcgaccg cctgattgag gtcggcgtcg ggcatcacca ccatgtggtt
     1621 cttggcgccg cccaaggcct gcacccgctt gccggagctt gcgccgcgcg tgtagatcga
     1681 gtgcgcgatc ggcgaggagc cgacgaagct caccgccttc acgtccggat cggcgagcag
     1741 cgcctccacg gcctccctgt caccctgcag gacgttgaat acgccaggcg gcaggcccgc
     1801 ctcgccgagc agctgcgcca tcaccaccgc ggcagaagga tcgcgctcgc tcggcttcag
     1861 cacgaagctg ttgccgcagg cgatggcgag cggaaacatc cagcacggca ccatgcaggg
     1921 aaagttgaag ggcgtgatcc ccgcgaccac gccgagcggc tggcgcagcg tccagttgtc
     1981 gatgccggtg gagacctgat cggtgaagcc gccattcagc agctgcggaa tgccgcaggc
     2041 gaactccacg atctcgatgc cgcgcgagac ctcgccctgc gcgtcg
//
LOCUS       DATLKL010000144         1542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_12754, whole genome shotgun sequence.
ACCESSION   DATLKL010000144 DATLKL010000000
VERSION     DATLKL010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12754"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VM713_01930"
     CDS             <1..185
                     /locus_tag="VM713_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HVH83050.1"
                     /translation="AIAPSLGGVHTLLTRPATTSHAGLSPEDRARLGINDGLLRLSVG
                     IEATEDLLEDFAQALG"
     gene            243..1154
                     /locus_tag="VM713_01935"
     CDS             243..1154
                     /locus_tag="VM713_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVH83051.1"
                     /translation="MSTDLTTPATTASATHAAGGWRTPLELTLLGAIWGGSFLFMRVA
                     AGDFGPLPLVETRLALGGLVLTPFLWRARAQFTARLWLRIAGIAAINSVIPFALFAWG
                     AERAPAGIGAITNAMTVMFTYLVAHFFFEERVGARRLVGLAAGFIGVAILASGKTAGP
                     GVWQAALAGTAASVCYGFGINLMRRYLTGYPSGAVAAANLITGSILLAPLALYTWPSR
                     PIPPASWTSAVLLGALCTGAAFLLYYRLVARIGAPRASTVTYLVPLFGVIWAWLVLGE
                     PLTVTMALAGALILSGVALSQQRATRG"
     gene            1273..>1542
                     /locus_tag="VM713_01940"
     CDS             1273..>1542
                     /locus_tag="VM713_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HVH83052.1"
                     /translation="MHALALAGARRRPRTARCRGLAALVDYGIETVIDLRWRAEVERH
                     PSPIPAALPQVHYRQVSLLTPTEDEWRERSADASKELWNCVVLEQV"
BASE COUNT          191 a          572 c          522 g          257 t
ORIGIN      
        1 cggcgatcgc gccgagcctc ggcggggtgc ataccctgct gacgcgtccg gccaccacct
       61 cgcacgcggg attgtcgccc gaggatcggg cgcgccttgg catcaacgac gggctgctgc
      121 gcctgtcggt cggcatcgag gcgaccgagg acctgctcga ggactttgcg caggcgctcg
      181 gttgaggaac ccaagcgcaa gtttcgggtg gcgaagcccc cggccctccc tcaggatgca
      241 gcatgtcgac tgacctcacc acacctgcga cgacagcgtc cgccacgcac gcagccggcg
      301 gctggcgcac gccgctcgag ctgacgctcc tcggggcgat ctggggcggc tcctttctct
      361 tcatgagggt ggcggctggg gacttcggcc cgctgccgct ggtcgagaca cgcctagcgc
      421 tgggcggtct cgtcctgacg ccgttcctgt ggcgcgcacg ggcgcagttc accgcgcggc
      481 tctggctgcg gatcgccggc atcgcggcga tcaactccgt gatcccgttc gcgctcttcg
      541 cctggggtgc cgagcgcgct cccgccggca ttggcgccat caccaacgcg atgacggtca
      601 tgttcaccta cctggtcgcg cacttctttt ttgaggaacg cgtcggcgcg cgacggctcg
      661 tcggcctcgc cgccggattc atcggcgtgg caatcctcgc gagcggcaag accgctggcc
      721 cgggcgtgtg gcaggcggcg ctcgccggca cggcagcctc ggtgtgctac ggcttcggca
      781 tcaatctgat gcgccgctat ctcaccggct acccctccgg cgcggtggcg gccgccaatc
      841 tcatcaccgg ctcgatcctg ctcgccccgc tggcgctcta cacctggccg tcgcggccga
      901 ttccaccggc ctcctggacg agcgccgtgc tccttggcgc gctctgcacc ggggcggctt
      961 tcctgctcta ttaccgcctc gtcgcgcgca tcggcgcccc gcgcgcctcc acggtcacct
     1021 acctcgtccc gctcttcggc gtgatctggg cgtggctggt actcggtgag ccgctcacgg
     1081 tcaccatggc cctggccggc gcgctgatcc tctcgggcgt cgcgctcagc cagcagcgtg
     1141 ctacaagggg atgatggcgg cgcgcgagct cacgacaccg agggaactgg cggcgcaccc
     1201 ccggcggcgg ctcgatttcc cggggctgct caacgcgcgc gacctgggcg gctgcccaac
     1261 gctcgatggc gggtgcacgc gctggcgctc gctggcgcgc gccgacgacc tcgcacagct
     1321 cgatgccgcg ggcttgccgc gctcgtcgat tacggcatcg agacggtcat cgacctgcgc
     1381 tggcgcgcgg aagttgagcg gcacccgagc ccgatccccg ccgccctgcc ccaggtgcac
     1441 taccgtcagg tttcgctcct cacgcccacc gaggatgaat ggcgcgagcg cagcgcggat
     1501 gccagcaagg agctgtggaa ctgcgtggtg ctcgagcagg tc
//
LOCUS       DATLKL010000145         2243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_137045, whole genome shotgun sequence.
ACCESSION   DATLKL010000145 DATLKL010000000
VERSION     DATLKL010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137045"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /gene="sufC"
                     /locus_tag="VM713_01945"
     CDS             <1..675
                     /gene="sufC"
                     /locus_tag="VM713_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HVH83053.1"
                     /translation="AGEVHAIMGPNGSGKSTLAYILAGRPGYAITSGTVHYLGHDLLA
                     LPPEERARQGLFLGFQYPVEIPGVNNVYLLKAAVNAVRKQQGLPEVDAFDFLALVRDK
                     MKLMHMEDSFLSRGVNEGFSGGEKKRNEVLQMLVLEPKLAILDETDSGLDIDALKVVA
                     AGVNSLRSPERSCVVVTHYQRLLEYVVPDVVHVLVRGRIARSGDRTLALELERRGYDW
                     VSGEAA"
     gene            683..2014
                     /gene="sufD"
                     /locus_tag="VM713_01950"
     CDS             683..2014
                     /gene="sufD"
                     /locus_tag="VM713_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HVH83054.1"
                     /translation="MSNPLTARITEQHASAARALAAHLPGAARRRAAMEALAVTGLPS
                     LRDENWKYANLRPLERASFTPAALTPPATGADAAFAAGLPARITDFMRYVFVDGVFAP
                     ALSDASAATAAQVMPLSARVDNPGAPPASLVPGRELGVDQRFALLNEAFATDGAAIDV
                     PAGPEAARLELIFLASAAAQQGASYPRVELRLEPRARLVLIERHLSAGAEASFVTSAV
                     SVELARGAALDHYRLQELSSRTTLFDTLDARLAQDSRYRLHAIGSGAAAARSTLRLQL
                     LGERADLALAVAALGDRHQVQDTYALVEHRAPHARTEETFRGIAAGRARVAFNGKIVV
                     AREAHGTDSRQSLRGLLAGPEAEIDVRPQLEIYTDEVRCSHGATAGKLDDNMLFYLLA
                     RGLDRDTAQRALKWAFLEDVIAKIEVAALRRQIEESLAGRMQDGAALRELI"
     gene            2014..>2243
                     /locus_tag="VM713_01955"
     CDS             2014..>2243
                     /locus_tag="VM713_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVH83055.1"
                     /translation="MAANLSPLTATPAYDAARVRRDFPILTTQVNGHPLVYLDSAASS
                     QRPLPVLRAVEDYETHSHANVHRGVHALSQAAT"
BASE COUNT          330 a          832 c          762 g          319 t
ORIGIN      
        1 gcgggcgaag tgcacgccat catggggccg aacggctcgg gcaagagcac cctcgcctac
       61 atattggcgg gccgccccgg ctacgcgatc acctccggca ccgtgcacta cctcggccac
      121 gacctgctcg cactgccacc cgaagagcgc gcgcggcagg gcctcttcct cggcttccag
      181 tacccggtcg agatcccggg cgtcaacaac gtctatctgc tcaaggccgc cgtcaacgcc
      241 gtgcgcaagc agcagggact gcccgaagtc gatgcgttcg acttcctcgc gctggtgcgc
      301 gacaagatga agctgatgca catggaggac agcttcctgt cgcgcggcgt caacgagggc
      361 ttctccggcg gcgagaagaa gcgcaacgag gtcctgcaga tgctggtgct ggaacccaag
      421 ctcgcgatcc tcgatgagac cgactccggc ctcgatatcg atgcgctcaa ggtggtggcg
      481 gcgggtgtga actcgctgcg ctcgcccgag cgctcctgcg tcgtggtgac gcattaccag
      541 cggctgctcg agtacgtcgt gcccgatgtg gtgcacgtgc tggtgcgcgg ccgcatcgcg
      601 cgcagcggcg atcgcacgct ggccctcgag ctcgagcggc gcggctatga ctgggtgagc
      661 ggcgaggccg cctgagacag cgatgagcaa tcctctcacg gcccgcatca ctgagcagca
      721 cgcgagtgcg gcacgcgcgc tcgcggcgca cctgcccggc gctgcgcggc gccgggcggc
      781 catggaagcg ctggcggtga ccggcctgcc gagcttgcgc gatgagaact ggaagtacgc
      841 gaacctgcga ccgctcgagc gggcgagctt cacccccgcc gccctcacgc caccggcaac
      901 gggcgcggac gccgcattcg ccgccggact cccggcgagg atcacggact tcatgcgcta
      961 cgtgttcgtc gatggcgtgt tcgctcccgc gctctccgac gcatccgctg cgaccgctgc
     1021 gcaggttatg ccgctctccg ctcgggtcga caacccgggc gccccgcccg cctcactcgt
     1081 gcctggccgg gagctcggag tcgaccagcg cttcgcgctc ctcaacgagg cgttcgcgac
     1141 cgacggcgcg gccatcgacg tgcccgcggg gcccgaggcc gcacgcctcg agctcatctt
     1201 cctggcgagc gccgcggcgc agcagggggc ttcctacccg cgcgtggagc tgcgcctcga
     1261 gccgcgggcg cggctggtcc tcatcgagcg ccacctgagc gccggcgccg aagcgagctt
     1321 cgtcacgagc gccgtcagcg tcgagctcgc ccgcggggcc gctctcgatc actaccggct
     1381 gcaggagctc agctcccgca ccaccctgtt cgacacgctc gacgccaggc tcgcgcagga
     1441 ctcccgctac cggttgcacg cgatcgggag cggtgcggcc gctgcccgct cgacgctgcg
     1501 gctgcagctc ctcggggagc gcgccgacct cgcgctcgcg gtggccgcgc tcggcgaccg
     1561 tcatcaggta caggacactt acgcgctggt cgagcaccgc gcgccgcacg cccgcacgga
     1621 agagacgttc cgcggcattg cggcggggcg cgcccgggtc gccttcaacg gcaagatcgt
     1681 ggtcgcccgc gaggcgcacg gcaccgactc gcgccagtcg ctgcgcgggc tgctcgcggg
     1741 cccggaggcc gagatcgacg tgcggccgca gctcgagatc tataccgacg aggtgcgctg
     1801 cagccatggc gcgaccgccg gcaagctcga cgacaacatg cttttctatc tcctcgcgcg
     1861 cggcctggac cgtgacacgg cgcaacgcgc cctcaagtgg gcgtttctcg aggacgtgat
     1921 cgccaaaatc gaggtggcgg cgctccgccg gcagatcgag gagagtctcg ccggccggat
     1981 gcaggacggc gccgcgctcc gggagctcat ctgatggcag ccaatctctc gccactcacc
     2041 gccacgcccg cctacgatgc ggcgcgcgtg cggcgcgact tccccattct cacgacgcag
     2101 gtgaacgggc acccgctcgt ttatctggac agcgccgcct cctcgcagcg cccgctgccg
     2161 gtgctgcgcg cggtcgagga ctacgagacg cactcgcacg ccaacgtgca ccgcggcgtg
     2221 cacgcgctca gccaggcggc gac
//
LOCUS       DATLKL010000146         1822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_12797, whole genome shotgun sequence.
ACCESSION   DATLKL010000146 DATLKL010000000
VERSION     DATLKL010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12797"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VM713_01960"
     CDS             complement(<1..437)
                     /locus_tag="VM713_01960"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVH83056.1"
                     /translation="MSVGPPLKMSHGVGPPLWLLLELTYRCPLHCVYCSNPTDFAATG
                     AELPTAEWLRVLREARALGAVQLGLSGGEPLLREDLEALVLEAHTLGFYINLITSGVG
                     MSEARLRSLKVAGLDHIQLSFQDSTRELNDFLSSTRTFERKAQV"
     gene            complement(434..712)
                     /gene="pqqD"
                     /locus_tag="VM713_01965"
     CDS             complement(434..712)
                     /gene="pqqD"
                     /locus_tag="VM713_01965"
                     /inference="COORDINATES: protein motif:HMM:NF002535.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HVH83057.1"
                     /translation="MSAVSAGARPAIAHGLRLQWEPAQEAHVLLYPEGMVKLNGSAGA
                     ILERCDGVRTVEEIVAELERAFGATDLSGDVSAFVELALERGWLELRA"
     gene            complement(709..1407)
                     /gene="pqqC"
                     /locus_tag="VM713_01970"
     CDS             complement(709..1407)
                     /gene="pqqC"
                     /locus_tag="VM713_01970"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HVH83058.1"
                     /translation="MSEPAWTRAEFDARLRERGRAYHIHHPFNVMLNSGKASREQIRG
                     WVANRFYYQISIPLKDAAILANCSERAVRRNWVQRILDHDGHGDDPGGIESWLRLAEA
                     VGLARAEVESLTLLLPGVRFAVDAYVSFARRAPWPEAVCASLTELFAPEIHRQRLAGW
                     PQHYPWIDPAGLEYFQSRMSRARRDVEFALAVTLERFDTRAAQERALEVLKLKLDILW
                     QMNDAMALGYGVTA"
     gene            complement(1404..>1822)
                     /locus_tag="VM713_01975"
     CDS             complement(1404..>1822)
                     /locus_tag="VM713_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVH83059.1"
                     /translation="PYSPHRAAPVAGDNVALVISDATSGRSAVYAPGLGRIEPALWSE
                     LEAAACVLVDGTFWSNEEMMAQGLSAKLARDLGHLPQSGAGGMLEWLAKLPPATRRIL
                     IHVNNTNPILDEDSPERAALERAGVEVAWDGMEIAL"
BASE COUNT          277 a          635 c          629 g          281 t
ORIGIN      
        1 acctgcgcct tgcgctcgaa ggtgcgcgtg ctggagagaa agtcgttcag ctcgcgcgtg
       61 ctgtcctgga acgacagctg gatgtgatcg agccccgcca ccttgagcga gcgtaggcgc
      121 gcctcgctca tgccgactcc cgaggtgatg aggttgatat agaagccgag cgtatgcgct
      181 tcaaggacca gcgcctcgag gtcctcgcgc agcagcggct ccccgcccga gagcccgagc
      241 tgcacggcgc cgagcgcgcg tgcctcgcgc agcacccgca gccactctgc ggtggggagc
      301 tcagctccgg tggcggcgaa gtccgtcggg ttggagcagt acacgcagtg cagcgggcag
      361 cggtaggtga gttcgagcag cagccaaagc ggtgggccca ctccgtgcga catcttcagc
      421 ggtgggccca cgctcatgcg cgcagctcca gccagcctcg ctcgagcgcg agctcgacga
      481 acgcgctgac atcgcccgaa agatccgtgg cgccgaaggc gcgctcgagc tcggcgacga
      541 tctcctcgac ggtgcgcacc ccgtcgcagc gctcgaggat tgcgccggcg ctgccgttga
      601 gcttcaccat gccctcgggg tagagcagca cgtgagcctc ctgcgccggc tcccattgca
      661 gccgcaggcc gtgcgcgatc gccggccgcg ccccggcgct caccgcgctc acgccgtcac
      721 tccgtacccg agcgccatcg cgtcgttcat ctgccagagg atatcgagct tgagcttcag
      781 tacctcgagt gcccgctcct gggcggcgcg cgtgtcgaag cgctcgaggg tcacggcgag
      841 ggcgaactcg acgtcgcggc gtgcgcggct catgcggctc tgaaaatatt cgagacccgc
      901 gggatcgatc cacgggtaat gctgcggcca gccggcgaga cgctggcgat ggatctccgg
      961 cgcgaacagc tccgtgagcg aggcgcacac cgcttccggc cagggtgcgc ggcgcgcgaa
     1021 gctgacatag gcatcgacgg cgaagcgcac accgggcagc aggagggtca ggctctcgac
     1081 ctcggcgcgc gcgagaccca ccgcctcggc gaggcgcagc caggattcga tgccgcccgg
     1141 atcatcgccg tggccatcgt gatcgaggat gcgctgcacc cagttgcggc gcaccgcgcg
     1201 ctcgctgcag ttggcgagga tcgcggcatc cttcagcggg atgctgatct ggtagtaaaa
     1261 gcggttcgcc acccagccgc ggatctgctc gcgactcgcc ttgccggagt tcagcatgac
     1321 gttgaacggg tggtggatgt ggtaggcgcg accgcgctcg cgcagcctgg cgtcgaactc
     1381 cgcgcgtgtc cacgccggct cgctcacagc gcgatctcca tcccgtccca cgcgacttcg
     1441 accccggcac gctcgagcgc ggcgcgctcg ggcgagtctt cgtcgaggat cggattggtg
     1501 ttgttcacgt ggatgaggat ccggcgcgtg gccggcggca gcttcgccag ccactcgagc
     1561 atgccgcccg cgccgctctg gggcaaatgc ccgaggtcgc gggcgagctt cgccgagagt
     1621 ccctgcgcca tcatctcctc attgctccag aaggtgccgt cgaccagcac gcaggcggcc
     1681 gcctcgagct cactccagag cgcgggttcg atcctcccga gcccgggtgc gtacaccgcg
     1741 ctcctgccgc tcgttgcatc gctgatcacg agcgccacgt tatcgccggc gaccggggct
     1801 gcgcggtgcg gcgagtaggg cg
//
LOCUS       DATLKL010000147         2020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_148346, whole genome shotgun sequence.
ACCESSION   DATLKL010000147 DATLKL010000000
VERSION     DATLKL010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148346"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="VM713_01980"
     CDS             <1..1338
                     /locus_tag="VM713_01980"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HVH83060.1"
                     /translation="VELLIGVYGSNEGLENTPSSSPGCLLPPPKRIVSDYLIAQRAKR
                     LGIPAIPGHRAVLTRALDSHSNPRRLHPGNARAQKILAADMRSRAPCLWATPCGRGCS
                     IRANYQSTTVHLPPALATGNLDITTDAMVYEVTLGPDGRATGVNFIDRTTGKHEHASA
                     RVVVLAASACESARILLNSKSAHFPDGLANSSGKVGRYLMDTVGSDVTGQVPLLESLP
                     PLNEDAADGHQLYTPWWLYQEQLAGKLGFARGYHIEFGGGKRMPGPGTGRGLEWLTGG
                     NYGAKFKEDVRRYYGSFVYFSGRGEMIPNENSYCEIDPGGVTDKWGIPVLRFHWQWSE
                     HETRQAAHMQRTFGEIIEAMGGKAQKIETDGARAIAPGGLIIHEVGGAIMGADSKTSV
                     TNQWCQAWDVPNLFVADGSVFPSNADKNPTLTIMAIAWRAADHILERMRRKEL"
     gene            1338..1976
                     /locus_tag="VM713_01985"
     CDS             1338..1976
                     /locus_tag="VM713_01985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HVH83061.1"
                     /translation="MDRRTTIKWMLAASAAWPVWRTGAGQGVTGGTALLRPPAAPGYG
                     SDPDLITAHKRGEFWPLTFSAAQRRLAAVLSDLIIPADEHSPSASSVGVVDFIDEWVS
                     APYPTQQGDRAVVLAGFSWLDAQGARRAGKPFAELGAATQTAICDSICDASWVTPERR
                     EATGFFALYRDLTAGAFYSSPAGRKDLGYIGNVPLTSFEGAPPELLKKLNLI"
BASE COUNT          352 a          683 c          676 g          309 t
ORIGIN      
        1 gtggagctcc tgatcggcgt ctatggcagc aacgagggcc tcgagaacac accgagctcc
       61 tctcccgggt gcctgttgcc cccgcccaag cgcatcgtga gcgattacct gatcgcgcag
      121 cgggccaagc gcctcggcat cccggcgatt ccaggtcacc gcgcggtact cacccgcgcg
      181 ctcgattccc acagcaatcc gcgccgcctc catcctggta acgccagggc tcagaagatt
      241 ctcgccgccg acatgcgcag ccgtgccccc tgcctgtggg cgactccctg cggacgtggc
      301 tgctcgatcc gcgccaacta ccagtcgacg accgtgcatc tgccgccggc gctcgccacc
      361 ggcaacctcg acatcacgac ggacgccatg gtgtacgagg tcacgctcgg gcccgacgga
      421 cgcgcgacag gggtcaattt catcgaccgc accaccggca agcacgagca cgccagcgcc
      481 cgcgtggtgg tgctcgcggc gagtgcctgc gaatcggcgc gcatcctcct caactcgaag
      541 agcgcacact ttccggacgg gctcgccaac tcgagcggca aggtcgggcg gtatctgatg
      601 gatacggtcg ggagcgacgt gaccgggcag gtgccgctgc tcgagagcct cccgccactc
      661 aacgaggacg ccgcggacgg tcaccagttg tatacgccgt ggtggctcta ccaggaacag
      721 ctcgcgggca agctcggctt cgcgcgcggt taccacatcg aattcggcgg cggcaagcgc
      781 atgccgggac ccggtacggg tcgcggcctc gaatggctga ccggcggcaa ctacggcgcg
      841 aagttcaagg aggacgtgcg ccgctattac ggatccttcg tgtacttctc gggacgcggg
      901 gagatgatcc cgaacgaaaa ctcttactgc gagatcgacc cgggcggcgt cacggacaaa
      961 tgggggatcc cggtgctgcg cttccactgg cagtggtccg agcacgagac gcgccaggcc
     1021 gcgcacatgc agcgaacctt cggcgagatc atcgaggcca tgggcggtaa ggcgcagaag
     1081 atcgagaccg acggtgccag ggccattgct ccgggagggc tcatcatcca cgaagtgggc
     1141 ggcgcgatca tgggagctga ctcgaagacc tcggtcacca accagtggtg tcaggcctgg
     1201 gacgtgccca acctgttcgt ggccgacggc tcggtatttc cctccaacgc cgacaagaat
     1261 ccgacgctca ccatcatggc gatcgcctgg cgggcggcgg atcatatcct cgagcgcatg
     1321 cggcgcaagg agctgtgatg gaccgccgca cgaccatcaa gtggatgctg gcggcgagtg
     1381 cggcctggcc ggtgtggcgc acgggggcgg ggcagggcgt gacgggtggc acggcgctgc
     1441 tccgaccgcc ggccgcgccg ggttatggca gcgatccgga ccttatcacg gcgcacaagc
     1501 gcggggagtt ctggccgctg acattcagcg cagcgcagcg gcggctcgcg gccgtgctct
     1561 cggacctcat cattcccgcg gatgagcact ccccgagcgc ctcaagcgtc ggcgtagtcg
     1621 atttcatcga cgagtgggtg agcgcgccgt acccgacgca acagggcgac cgcgcagtcg
     1681 tgctggcggg attttcctgg ctggacgcgc aaggcgcgcg gcgcgcaggc aagccatttg
     1741 cggagctcgg cgccgccacg caaaccgcca tctgcgattc gatctgcgat gcgtcgtggg
     1801 tgacgcccga gcggcgcgag gcgacgggct tcttcgcgct ctaccgggat ctgaccgccg
     1861 gcgccttcta ctcgagtccc gccgggcgca aggatctcgg ctacatcggc aacgtgcccc
     1921 tcacgagctt cgaaggcgcg ccgcccgagc tgctgaagaa actgaacctg atctgaccgg
     1981 agcaactcat gaataagact cagctctgcg cggcgctgct
//
LOCUS       DATLKL010000148         1618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_182196, whole genome shotgun sequence.
ACCESSION   DATLKL010000148 DATLKL010000000
VERSION     DATLKL010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182196"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..319
                     /locus_tag="VM713_01990"
     CDS             47..319
                     /locus_tag="VM713_01990"
                     /inference="COORDINATES: protein motif:HMM:NF017509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83062.1"
                     /translation="MKLDRQRLNERLDDLVASYPNPDEARRAYVQNPEAMRQLESAVL
                     EDQVVDWALKAARMTERPMTFKELTGFGPAAGGAEQHAQHSEVSGT"
     gene            331..960
                     /gene="clpP"
                     /locus_tag="VM713_01995"
     CDS             331..960
                     /gene="clpP"
                     /locus_tag="VM713_01995"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HVH83063.1"
                     /translation="MNLVPIVVEQTARGERAYDIYSRLLKERVVFVVGPVDDYMANIV
                     VAQMLFLESENPEKDVALYINSPGGVVSSGLAIYDTMQFIKPDVSTICIGQAASMGAV
                     LLAGGTRGKRYCLPHARVMIHQPAGGFQGQAADMEIQAKEMLETRDRLNKILAKHTGQ
                     SVERIKQDTDRDRFMDWQEAVRYGLIDKVLEQRGEAGGPSGAGTPGKAG"
     gene            1153..>1618
                     /locus_tag="VM713_02000"
     CDS             1153..>1618
                     /locus_tag="VM713_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HVH83064.1"
                     /translation="MSEDSRNRHDDGKLLYCSFCGKSQHEVRKLIAGPSVFVCDECVE
                     LCNDIIREELEDRAERARDKLPKPHEIKKVLDEYVIGQERAKKVLSVAVYNHYKRLQT
                     RSGTARTKEEVEIAKSNILLIGPTGCGKTLLAETLARLLNVPFTIADATTLTE"
BASE COUNT          334 a          516 c          527 g          241 t
ORIGIN      
        1 cgtggcgctc gggctcctca tgagccacat cgtctagtcg cagggcttga aactcgaccg
       61 ccagcgcctc aatgagcgtc tcgacgacct ggtggcgagc tacccgaatc cggatgaggc
      121 gcggcgcgcg tacgtgcaga accccgaggc gatgcgccag ctcgaatccg cggtcctcga
      181 ggaccaggtg gtcgactggg cgctgaaggc cgcgcgcatg accgagcggc cgatgacatt
      241 caaggaactg accggcttcg ggccggcggc gggcggcgcc gagcaacacg cacagcacag
      301 cgaagtgagt ggcacatgaa cgaacgcggc ttgaacttag tccccatcgt ggtcgagcag
      361 accgcacgcg gcgagcgcgc ctacgacatc tactcacgcc tcctcaagga gcgcgtcgtg
      421 ttcgtggtcg gcccggtgga tgactacatg gcgaacatcg tcgtcgcgca gatgctcttc
      481 ctcgagtcgg agaacccgga gaaggacgtc gcgctctaca tcaactcccc cgggggcgtg
      541 gtgagctcgg gacttgcgat ctatgacacc atgcagttca tcaagcccga cgtcagcacg
      601 atctgcatcg gccaggcggc cagcatgggc gcagtgctct tggccggcgg cacccgcggc
      661 aagcggtact gcctgccgca cgcccgcgtc atgatccatc agcccgcggg cggctttcag
      721 gggcaggccg ccgacatgga gatccaggcc aaggagatgc tcgagacgcg ggaccgactt
      781 aacaagatcc tcgccaagca caccggccag agcgtcgagc ggatcaagca ggacacggac
      841 cgggaccgct tcatggactg gcaggaagcg gtccgctacg gcctgatcga caaggttctg
      901 gaacagcgcg gcgaggcggg cgggccgtcc ggggcgggaa cccccggcaa ggcgggctga
      961 agcgggctct caggcgctgc cggagggtcg aatttgtaaa gtagtgaaac ccgtcataca
     1021 aaccatgccc aaacaggtag ttagacgtga cggtgctgct ttgcgtcgcg acggcgcacc
     1081 tgctataaga aaattgcaat gaacccgctt agccagggta ggcgtgggac gagaggttta
     1141 tgaggcccgc agatgagtga agactcccgc aaccgtcacg atgacgggaa gctcctgtac
     1201 tgctccttct gcgggaagag tcagcacgag gtgcggaagc tcatcgccgg cccctcggta
     1261 ttcgtctgcg acgaatgcgt cgagctctgc aacgacatca tccgcgagga gctcgaggat
     1321 cgcgccgagc gcgcccgcga caagctcccg aagccccacg agatcaagaa ggtcctcgac
     1381 gagtacgtga tcggccagga gcgcgccaag aaggtgctct cggtcgcggt ctataaccac
     1441 tacaagcggt tgcagacccg ctcgggcacc gcccgtacca aggaagaggt cgagatcgcc
     1501 aagagcaaca ttctgctcat cggacccacc ggttgcggca agacgctcct cgccgagacc
     1561 ctcgcccggc tcctcaacgt gccgttcacg atcgcggatg cgacgaccct caccgagg
//
LOCUS       DATLKL010000149         1614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_46740, whole genome shotgun sequence.
ACCESSION   DATLKL010000149 DATLKL010000000
VERSION     DATLKL010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46740"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VM713_02005"
     CDS             complement(<1..1589)
                     /locus_tag="VM713_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83065.1"
                     /translation="MNRHNILIAALCGLAVAASHAALAQHQPAGAYADLSWRMIGPMR
                     GGRTHAVAGVPSQPNVFYVGAVNGGLWKTGDAGRTWQPIFDAQPTQSIGAAAIAPSDP
                     NIIYVASGEGLRRPDLSVGDGIYRSADGGRTWTHGGLTGGEQIPELAVDPRDPNHLFA
                     AVLGHPYGANEERGIYRSRDGGTTWERVLYKDADTGGSAVALDPAHPDVVYAALWQSR
                     LGPWEDKNEFQGTNGGLFKSSDGGTTWKRLSEGLPENLTQINFAIAASAPQRIYAVAG
                     TTEPGDYSSAAGLGVFRSDDAGEHWTRITTDPRAALRIGGGDLTVLRVDPTNADVLYS
                     MGIVTMKSADGGKTWSPLRGAPGGDDYQNMWINPTDSRILALTSDQGAVISVNGGESW
                     SSWLNQPTAQLYHVSVTPTFPYRVCSGQQESGSVCIATRGNDGSITDHEWHPVGAIEY
                     GYVAPDPLDPDVIYGGGRTEVSKFHVSTGQVQNVTPAPVRGPDVRADRTQPLMFSPRN
                     PHVLYYALNRLWKTSDGGMTWS"
BASE COUNT          224 a          537 c          580 g          273 t
ORIGIN      
        1 gaccaggtca tgccgccgtc gctggtcttc cagagccggt tgagggcgta gtagagaacg
       61 tgcgggttac gcggcgagaa catcagcggc tgtgtgcggt cggcgcgcac gtcgggcccg
      121 cgcaccggag ccggcgtgac gttctgtacc tgcccggtag aaacgtggaa tttcgacacc
      181 tccgtgcggc cgccgccgta gatgacatcc ggatcgagcg gatcgggagc gacgtagcca
      241 tattcgatcg cgcccacggg gtgccactcg tgatcggtga tcgagccgtc gttgccgcgc
      301 gtggcgatgc acaccgagcc gctctcctgc tggccggagc aaacccggta cggaaatgtc
      361 ggggtgacgc tcacgtggta gagctgggcg gtcggctggt tgagccagct gctccagctc
      421 tcgccgccgt tcacgctgat gaccgcgccc tgatcgctgg tgagggcgag gatgcgggag
      481 tccgtggggt tgatccacat gttctgatag tcgtcgccgc ccggcgcgcc ccgcagcgga
      541 ctccaggttt tgccgccgtc ggcggacttc atggtgacga tgcccatact gtagaggacg
      601 tcggcgttgg tcgggtcgac gcgcagcacc gtcagatcgc cgccgccgat gcgcagcgcc
      661 gcgcgcggat ccgtcgtgat gcgcgtccag tgctcgcccg catcgtcgga gcggaacacg
      721 ccgagcccgg ccgccgagga gtagtcgccg ggctcggtag tcccggcaac ggcgtagatt
      781 cgctgcggcg cgctcgccgc gatcgcgaaa ttgatctggg tgaggttctc cggcagcccc
      841 tcgctcaaac gcttccaggt cgtgccgccg tcactcgact tgaagagtcc gccgttggtg
      901 ccctggaact cgttcttgtc ttcccacggt ccgaggcgcg actgccacag cgccgcgtac
      961 accacgtcgg ggtgcgcagg atcgagcgcg accgcggagc cgccggtgtc ggcgtccttg
     1021 taaaggaccc gttcccaggt ggttccgcca tcgcgcgagc ggtagatgcc acgctcctca
     1081 ttggcgccgt aagggtggcc aaggaccgcg gcgaacaggt ggttgggatc gcgtgggtcg
     1141 acggcgagct cggggatctg ctccccgccg gtgaggccgc cgtgcgtcca ggtgcgcccg
     1201 ccgtcggcgg agcggtagat gccgtctccg actgaaaggt ccggccgccg cagcccctcg
     1261 ccgctcgcca catagatgat gttggggtcg gagggcgcga tcgcggccgc gccgatcgat
     1321 tgggtcggct gcgcgtcgaa gatgggctgc caggtgcggc cggcgtcgcc cgtcttccat
     1381 agcccgccgt tgaccgcgcc cacgtagaag acattcggct gcgacggcac gcccgccacc
     1441 gcgtgcgtgc ggccgccgcg cattggtccg atcatgcgcc agctcaggtc ggcgtacgcc
     1501 cccgcgggct ggtgctgcgc gagcgcagcg tggctggccg cgaccgccag gccgcacagc
     1561 gcagcgatca ggatattgtg tctgttcatc gtgtagctcc cgggtgcccg gccg
//
LOCUS       DATLKL010000150         3260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_1786, whole genome shotgun sequence.
ACCESSION   DATLKL010000150 DATLKL010000000
VERSION     DATLKL010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VM713_02010"
     CDS             complement(<1..1144)
                     /locus_tag="VM713_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HVH83066.1"
                     /translation="MSVKNKLIWAAIAVLGAVAFGIVALTRNEPVNAMWLVIAALCVY
                     VIGFRFYGLFIANTVLGVNPARQTPAYRRNDGLDYVPTNPYVLFGHHFAAIAGAGPLV
                     GPVLAAQMGYLPGTLWLLAGVVFAGAVQDMVVLFLSTRRDGRSLGDIIRSEMGPTAGT
                     IAGVGVLLICVIILAVLALVVVNALKSSPWGTSTVSCTIPIAIIMGLYGRFLRPGRIG
                     EMSLIGAVLLLAALIFGRNVADSPALAHYFTLSGPTLALLIIGYGYVASVLPVWLLLA
                     PRDYLSTFLKVGTVALLAVGVLLAHPNLQMPAITKFIDGSGPVWSGSLFPFLFITIAC
                     GAVSGWHSLISSGTTPKMIESEKHIAYIGYGGMLMESFVGIMAMIGA"
     gene            complement(1356..>3260)
                     /locus_tag="VM713_02015"
     CDS             complement(1356..>3260)
                     /locus_tag="VM713_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVH83067.1"
                     /translation="VVDDNPSIHDDYRKILAGQDAGKLSAAEAALFGEQQPAVERPSF
                     DVDSAMQGRDGVERARTALAEGRPYSVAFVDMRMPPGWDGLETIEHLWAIDPEIQVVV
                     CSAYTDYDWLELLSRLGHSDKLIVVKKPFEPIEILQCASALSRKWANARALKQHVESL
                     ELVVTDRTLGLEAANRKLRHLASHDALTGLPNRLLLDDRITQTIAQADRQLHEFAVLV
                     IDLDRFKIINDSLGHRAGDELLREVAQRLKSAVRTVDTTARLGGDEFVILLGPPITHA
                     EALAVARRVIELMEPSMRLLGMDVHISPSIGMAFYPRDGRTVDTLLAHADAAMYTAKE
                     RGRNNVQCYAEGMTAGSQERMQLESELRQALQSGQFELHYQPKVETSTGRINSAEALI
                     RWRHPERGLLLPADFIGIADDCGLLDDIGEWVLHEACRQAKAWQDAGLPPLRVAVNLA
                     PSQFRLTNLVDQIRRALDAAQLDPRFLEVELTESAVMSDAEESIRILEAVSSMGVLVS
                     VDDFGTGYSSMSYLRRFPIDKLKIDRCFVEDMTRRSEDASIVRAIISLAHSLRLKVIA
                     EGVETPEQLALLAQLGCDQYQGFYFSAALTADKFMSLVRQSVAHQPQRDADDVERTQS
                     KLAALGRPRR"
BASE COUNT          565 a         1065 c         1115 g          515 t
ORIGIN      
        1 aggcgccgat catcgccatg atccccacga aggactccat cagcatcccg ccgtagccga
       61 tgtaggcgat gtgcttctcg ctctcgatca tcttcggtgt ggtgccggag gaaatgagcg
      121 agtgccagcc ggacaccgca ccgcacgcga tggtgatgaa caggaacggg aacaggctcc
      181 cggaccacac cggtccgctg ccgtctatga acttggtgat ggcgggcatc tgcaggttcg
      241 ggtgggcgag cagcacgccg accgccagca gcgcaaccgt gcccactttg aggaacgtgg
      301 acaggtagtc gcgcggcgcc aagagcagcc agaccggcag caccgaggcg acgtagccat
      361 agccgatgat gagcaacgcc agcgtcgggc ccgacagcgt gaagtaatga gccagcgccg
      421 ggctatcggc aacgttacga ccaaagatca gcgccgcgag cagcaacacc gccccgatga
      481 gcgacatctc gccaatgcgg cccgggcgca ggaagcggcc gtatagcccc atgatgatgg
      541 cgatcggtat ggtgcaggaa acggtggagg tgccccaggg gctgctcttc agcgcgttca
      601 cgaccaccag tgccagcacc gcgagaatga tcacgcatat gagcaggacc cccacgccgg
      661 cgatcgtgcc ggccgtcggg cccatctcgg agcggatgat gtcgccgagc gagcggccat
      721 cgcgccgcgt cgagaggaac agcacgacca tgtcctgcac tgcgccggcg aacaccacgc
      781 cggcgagcag ccagagcgtg ccgggcagat agcccatttg tgccgccagt accggaccaa
      841 ccaacggccc ggcgccggcg atggccgcga aatgatggcc gaacaggacg tacgggttcg
      901 tcggcacata atcgaggcca tcgttgcgcc ggtaggccgg agtctggcgc gcggggttca
      961 ctcccagcac cgtgttggcg atgaacaggc cgtagaaccg aaacccgatc acgtagacgc
     1021 atagcgccgc aatcaccagc cacatcgcgt tgaccggctc attgcgagtc agcgcgacga
     1081 tgccgaaggc cactgcgccc agcaccgcaa tggctgccca gatcagcttg ttcttgaccg
     1141 acatcgctcg ctcctagttt cttgtacgcg accgggctgc cccgcacggg acatccggcc
     1201 ccttagttct agtggccgcg atcaactaca gcagattatc tctccccggc aggcatagcc
     1261 actggagggt cgccgggcgg gaaaccgcaa tcggctcagg gcgttgcggc gaggctcagt
     1321 gccccagggt gggtgttggc agggcgccgc gcgcctcagc ggcggggccg tccgagtgct
     1381 gcgagcttgc tttgggtgcg ctcgacgtcg tccgcgtcgc gctgcggctg gtgggccacc
     1441 gactgccgca ccaggctcat gaacttatcg gcggtgagcg ccgcactgaa gtagaagccc
     1501 tggtactgat cgcagccgag ctgggcgagc agggcgagct gctcgggagt ctcgacgccc
     1561 tcggcgatca ccttgaggcg caggctgtgg gccagcgaga tgatggcgcg cacgatcgag
     1621 gcgtcctccg aacgccgcgt catgtcctcg acgaagcagc ggtcgatctt gagcttgtcg
     1681 atcggaaaac ggcgcaggta gctcatgctc gagtagccgg tgccgaagtc atcgaccgac
     1741 accagcacgc ccatgctgct gaccgcctca agaatgcgga tcgactcctc ggcatcgctc
     1801 atgaccgcgc tctcggtgag ctcgacctcg aggaaccgcg ggtcgagctg ggcggcatcg
     1861 agggcgcggc gtatctggtc caccaggttg gtgagccgga attgcgaggg cgcgagattc
     1921 accgcgacgc gcagcggcgg gagtcccgcg tcctgccagg ccttggcctg ccggcaggcc
     1981 tcgtgcagca cccattcgcc gatgtcgtcg agcagcccgc agtcgtcggc gatgccgatg
     2041 aagtccgccg gcagcagcag cccgcgctcg ggatggcgcc agcggatgag cgcttcggca
     2101 ctgttgatgc ggccggtgga ggtctcgacc ttgggttgat agtgcagctc gaactggccg
     2161 ctctgcagcg cctggcgcag ctcgctctcg agctgcatgc gctcctgcga gccggcggtc
     2221 atgccctcgg cgtagcactg cacgttgttg cggccgcgct cttttgccgt gtacatggcg
     2281 gcgtccgcat gcgccagcag cgtatcgacc gtgcgtccgt cgcgcgggta gaacgccatg
     2341 ccgatgctcg gcgagatatg cacgtccatg ccgaggagcc gcatcgatgg ctccatgagc
     2401 tcaatcacgc gccgcgcgac cgcgagcgct tccgcgtggg tgatgggcgg gcccaacagg
     2461 atcacgaact cgtcgccgcc gaggcgggcc gtcgtgtcga ccgtgcgcac ggcgctcttc
     2521 aggcgctgcg caacctcgcg cagcagctca tcgccggcgc gatggccgag cgagtcattg
     2581 atgatcttga agcgatcgag gtcgatcacc agcacggcga actcgtgcaa ctggcggtcc
     2641 gcctgggcga tcgtctgggt gatccggtca tcgagcagca gccggttggg gagcccggtg
     2701 agcgcgtcgt gcgaggcgag atggcgcagc ttgcggttgg ccgcctcgag tccgagggtg
     2761 cggtcggtca ccaccagctc gaggctctcg acgtgttgct tgagggcgcg cgcgttggcc
     2821 catttgcggc tgagcgcgct cgcacactgc agaatctcga tgggctcgaa gggcttcttg
     2881 acgacgatca gcttgtcgga gtgcccgagc ctcgagagca gctcgagcca gtcgtaatcg
     2941 gtgtaggccg agcagaccac gacctggatc tccggatcga tggcccacag gtgctcgatc
     3001 gtctcgagcc cgtcccaccc gggaggcatg cgcatgtcga cgaaggcgac cgaatagggg
     3061 cggccctcgg cgagggcggt gcgcgcccgc tcgacgccgt cacgcccctg catggcggaa
     3121 tcgacgtcga agctcgggcg ctcgaccgcg ggctgctgct cgccgaacag cgccgcttcc
     3181 gcggccgaca gcttgccggc gtcctggccc gcgagaatct tgcggtaatc gtcatggatc
     3241 gacgggttgt cgtcgaccac
//
LOCUS       DATLKL010000151         1848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_80674, whole genome shotgun sequence.
ACCESSION   DATLKL010000151 DATLKL010000000
VERSION     DATLKL010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80674"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            736..1125
                     /locus_tag="VM713_02020"
     CDS             736..1125
                     /locus_tag="VM713_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVH83068.1"
                     /translation="MLSPQLLGVLRAWWREGRRLGVLLPRGWLFPSRNPVDPLSTRQL
                     NRAVHAAAEAAGIKKRVSPHTLRHSFATHLLEQDTDIRVIQVLLGHAKLDTTARYTRV
                     ANTTIRTVTSPLDRLAPLTEGRPRPGV"
     gene            1135..>1848
                     /locus_tag="VM713_02025"
     CDS             1135..>1848
                     /locus_tag="VM713_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HVH83069.1"
                     /translation="MGRSKLEVADIFHRHGAAWRRANAGHISLGQLKVMSAIEHCRSA
                     ALGGHVERCTDCGHTRISYNSCRNRHCPKCQGAAARDWLADREADLLPVGYFHVVFTL
                     PAEIAPIAYQNKAVVYDLLFRTAAETLLTIAADPEHLGARIGATAVLHSWGATMTHHP
                     HVHMIVPGGGISLDGTHWIHCRPRFLVPVPVLSCLFRRLFLAGLADAYAAGRLVFFGE
                     IKYLRSQEAFAACLAPLRRK"
BASE COUNT          298 a          645 c          590 g          315 t
ORIGIN      
        1 cgactatcgg ctgataactg ggattcggtg atcgtccgct atgggtcaaa gaacgaaccc
       61 gctctcgcgg gagggcgcgc gtcaccgcca gtgcggtatc aggcgggcgg tgaacggtag
      121 ggggtgacca tcggggcatc cgatcccgag cattgaaggt ccatcaacct tcagctcgag
      181 agaggagcct ccgatgacct cgcagccatc cccccggccc atcagcccgc tgcgcgccca
      241 catgatcgag gacatgatgg tgcgcggctt cgccgagaag acgcgtcagg actacgtccg
      301 gcacgtgcaa gcgtttgccg ccttcatcgg ccgctcaccc gcgacggcga cgccggggga
      361 tgtgcgcggt ttccagctgc accagaggca gagcggcatg caaccgccga gcatcaacac
      421 ggcggtctcg gcactgcgtt ttctgtttac cgtgacgctc gaccggccgg acttggcccg
      481 acggctcacg gtggtgcgcc agccacgccg gctgccggtg gtgctgagcg tcgaggaggt
      541 ggcgctgctg atccaagcgg cgccgggacc gaagtacagg gcggcgttcg ccaccgccta
      601 tggcgccggt ctgcgcgtct ctgaagtcgt cgcgctcaag gtcggcgaca tcgactccga
      661 gcgcatgctg ctgcgagtcg agcagggcaa ggggcgcaag agcctgccct cgcgaaagcg
      721 ggggatcgcc acgccatgct gtcgccgcaa ctgctcggag tgctgcgcgc gtggtggcgc
      781 gagggccgac ggctcggtgt attgctgccg cgcggctggc tgttcccaag ccgcaatccg
      841 gtcgatccgc tgtcgacccg tcagctcaac cgcgccgttc acgccgccgc cgaggcggcc
      901 gggatcaaaa agcgggtgtc gccgcatacc ctgcggcaca gctttgccac ccatctcttg
      961 gaacaggata ccgacatccg tgtcatccaa gtgctgctcg gccacgccaa gctcgacacc
     1021 accgcccgtt acacccgtgt cgccaatacc acgatccgca ccgtgaccag cccgctcgac
     1081 cggctcgcgc cactgaccga aggcaggccg cgacccgggg tgtagctggc ggccgtgggc
     1141 cgctcaaagc tggaggtcgc agacatcttc catcgccacg gggccgcctg gcgccgagcc
     1201 aatgctgggc atatcagcct tggccagctg aaggtgatgt cagccattga gcactgccgc
     1261 tcggctgctc tcggcggcca tgtcgaacgt tgtacagatt gcggccacac tcggatctcc
     1321 tacaactcct gccgcaaccg ccattgtccc aagtgccagg gcgcagcggc gcgcgactgg
     1381 ctcgccgacc gcgaggccga cctgctgccg gtcggctact tccacgtggt cttcactttg
     1441 ccggccgaga tcgccccgat cgcttaccag aacaaggcgg tggtctacga cctgctgttc
     1501 cgcacggcgg ccgagacgct gcttaccatt gccgctgatc ccgaacacct cggcgcccgc
     1561 atcggcgcca ctgccgtgct ccacagttgg ggcgcgacga tgacccacca tccgcatgtc
     1621 cacatgatcg tgccgggcgg tgggatctca ttggacggga cccattggat acactgccga
     1681 ccccgtttcc tggtgccggt gccggtgctc tcctgcctgt tccgccgcct cttcctggcc
     1741 gggctcgccg atgcctacgc agctggtcgg ctggtcttct ttggcgagat caagtacctg
     1801 cgttcccagg aggcattcgc cgcctgtctc gcgccactca gaagaaag
//
LOCUS       DATLKL010000152         3288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_58132, whole genome shotgun sequence.
ACCESSION   DATLKL010000152 DATLKL010000000
VERSION     DATLKL010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58132"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..475
                     /locus_tag="VM713_02030"
     CDS             83..475
                     /locus_tag="VM713_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVH83070.1"
                     /translation="MARATADIGTINYTTALVAGHHQLTADEGAALGGEDAGPAPHEL
                     LCAALAACTAITLRMYAERKTWPLQAAHVDVLFQHEGEKRTMTRRLRLAGDLDEAQRA
                     RLADIAERTPVTLTLKQGVAITTILEPR"
     gene            complement(478..2070)
                     /locus_tag="VM713_02035"
     CDS             complement(478..2070)
                     /locus_tag="VM713_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH83071.1"
                     /translation="MANPKALPCDELAIRSSVSTPACGPARYWVLAVAVLGSTLSYVD
                     ESVVNVALPAIEADLRTTLPAMQWVINAYTLCMSALLLIGGAAADRFGRRRMFVIGAS
                     IFAAGSLGCGLAPSVAMLLGARAVSGIGAALLVPCSLALIGAAFDEKERGAAIGIWSG
                     AAAIAAGAAPLLGGGLLDHWSWRVIFLINPLLAIPTLWIALRRVPENRDPDAPSGIDW
                     QGSALGFAGLGVLVYGLIASSELGWTHPAVVVALAAGTGLLLLFLLVERRSPAPMMPL
                     DLFRSPTFSGVNLLTLLLYGGLGGGFFFLPFLLIQARGDSATAAGAAYLPFTVVVSLL
                     SRWSGGLIDRFGARWPLIIGPAIAAGGFAMLALSRHPYWTILLPMTVLGFGMAITIAP
                     LTTAVLNAVPAHRTGVASGINNAVASVGSLLVIAVLGSLAVGTMSHALERHLERPPAS
                     PAVRLAVETARGGFVIPALPASLAEEDRRRARAIIAASIDDTVRRMLWIAAALACAGV
                     LSAVATIPGGRRGPPRSTPSRQ"
     gene            complement(2077..>3288)
                     /locus_tag="VM713_02040"
     CDS             complement(2077..>3288)
                     /locus_tag="VM713_02040"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HVH83072.1"
                     /translation="QAVCWSGAGLALLLTLNLLPRLSLLFLYILYLSLLYAGQVFMTY
                     QWDTFLLETGFLALLLSCTITPGIWLLRWLLFRFMFMSGAVKLLSADPSWWNLSALSY
                     HFLTQPLPTPLAWYAAHQPPALLAFATAATFVVELGLPFLIFCPRRLRFGAALGILLL
                     QSCILLTGNYNWFNLQTMVLCLILFDDAALQSFLPQRLWARLPARVPLRRTPMALAIA
                     VGALAALLVGCSLIEMDLRFGGQPPAVARTVDALIEPLHLVSSYGLFAVMTTTRNEIV
                     IEGSNDGVEWREYEFRYKPGDVGRRPSWNIPHQPRLDWQMWFAALEDPRRLQWFWLFL
                     EKLLHNEPAVTSLLARNPFPDKPPVQIRAKFYDYSYSDAGEKARGIWWRRRLLGLYFP
                     QVRLRADQPAH"
BASE COUNT          633 a         1063 c         1178 g          414 t
ORIGIN      
        1 cattgcagac tcctcgagaa tcgcattcta gcgcgtcgcc tgccagactt gcggaggctc
       61 ccgcggccgg aggagaagcg gcatggcgcg tgcaacggca gacatcggca ccatcaacta
      121 cacgaccgcg ctcgtggcgg gccatcacca gctgaccgcc gacgaaggcg cggcgctcgg
      181 gggtgaggac gcaggtcccg ctccgcacga gttgctgtgc gcggcgctcg ccgcctgcac
      241 cgcgatcacg ctgcgcatgt acgccgagcg caaaacctgg ccgctgcagg cggcgcatgt
      301 cgacgtgctg ttccagcacg agggggagaa acgcacgatg acgcgccggc tgcggctcgc
      361 aggagacctc gatgaggcgc agcgtgcgcg cctcgccgac atcgccgagc gtaccccggt
      421 caccctgacc ctcaagcagg gtgtcgccat cacgacgatc ctcgaaccgc gctgagctta
      481 ttggcgtgag ggggtcgacc tcggcggtcc gcgtctgccc ccggggatcg tggcgaccgc
      541 ggacagtaca ccggcgcacg cgagggccgc cgcgatccac agcatccgcc gcaccgtatc
      601 gtcgatcgat gcggcgatga tggcgcgggc acgccgacgg tcctcctccg caaggctcgc
      661 cgggagcgcc gggatgacga atccgccgcg cgcggtctcg acggcgagcc gtacagcggg
      721 agaggctggc gggcgctcga ggtgccgctc gagcgcgtgg gacatggtcc ccacggcgag
      781 actgccgagt acggcgatta ccaggaggct gccgacggac gccacggcgt tgttgatgcc
      841 cgaggcgacg ccggtccggt gagcgggcac tgcgttcagc acggccgtgg tgagcggggc
      901 aatcgtaatg gccatcccga agccgagcac ggtcatcggc aggaggatgg tccagtaggg
      961 atggcggctc agtgcgagca tcgcgaagcc gccggcggcg atcgccggcc cgatgatgag
     1021 cggccagcgt gcgccgaacc ggtcgatgag tccgcccgac cagcgcgaca gcagactcac
     1081 gaccacggtg aacggcaggt acgcggctcc agcggccgtc gcggaatcgc cgcgtgcctg
     1141 aatcagcagg aacggaagga agaaaaaccc gccgccgagg ccgccgtaga gcaggagtgt
     1201 cagcaggttg acgccgctga aggtcggcga gcgaaacaag tcgagcggca tcatcggcgc
     1261 ggggctgcgt cgctcgacca gaaggaacag aagcagcagg ccggtgcccg ccgcgagagc
     1321 cacgactacc gcaggatgag tccaaccgag ctccgaggaa gcgatcagtc cgtacacgag
     1381 gaccccgagc cccgcgaacc cgagggcgct tccctgccag tcgatgccgg acggcgcatc
     1441 cggatcgcgg ttctccggga cacggcgcag cgcaatccac agcgtgggga tcgcgagcag
     1501 cgggttgatg aggaagatca cccgccatga ccagtggtcg agcagcccgc cgccgaggag
     1561 cggggcggcc ccggcggcga tcgctgcagc acccgaccag atgccgatcg cggctccgcg
     1621 ttccttctca tcgaaggcgg caccgatgag cgccagcgag caggggacga gcagggcggc
     1681 gccgatcccg gaaacggcac gcgccccgag cagcatcgcg acgctcggcg ccaggccgca
     1741 gccgagcgat ccggcggcga agatgctcgc tccgatgaca aacatgcgcc gccggccgaa
     1801 gcgatccgcc gcggcgccgc cgatcagaag taacgccgac atgcagagcg tgtaggcgtt
     1861 gatcacccat tgcatcgccg ggagcgtcgt gcgcaggtcg gcctcgatgg cgggcagggc
     1921 cacgttgacc accgattcat cgacatacga cagggtggac ccgaggactg cgactgcgag
     1981 cacccagtac ctggccgggc cgcaggcggg ggtactcacg ctcgaccgga tggcgagctc
     2041 atcacacggc agggctttgg gattcgccat gagtgctcaa tgcgccggct ggtccgcccg
     2101 cagtcgcacc tggggaaaat acaggccgag caggcgccgt cgccaccaaa tgccgcgggc
     2161 cttctcgccg gcgtcgctat aggagtagtc gtagaacttg gcgcggatct ggacgggcgg
     2221 cttgtccgga aaggggttcc tggcgaggag cgaggtcact gccggctcat tgtgcaggag
     2281 tttttcgaga aacagccaga accactgcag ccgccgcgga tcctcgaggg cggcaaacca
     2341 catctgccag tcgaggcgcg gctggtgtgg aatgttccag gacggccgcc gccccacatc
     2401 gcccggcttg tagcggaact cgtattcgcg ccactcgacg ccatcgttcg agccctcgat
     2461 gacgatctcg ttgcgggtgg tcgtcatgac ggcaaagagg ccgtaggagc tcaccaggtg
     2521 cagcggctcg atgagcgcat ccacagtgcg tgcgacggcg ggcggctggc cgccgaaacg
     2581 caggtccatc tcgatgaggc tgcagccgac cagcaacgca gcgagcgcgc cgaccgcaat
     2641 ggccagcgcc atgggggtcc gacggagcgg tacccgtgcc ggcagccgtg cccacaggcg
     2701 ctgcggcagg aagctctgca aggccgcgtc gtcgaacagg atcaggcaca gcaccatcgt
     2761 ctggaggttg aaccagttgt aattgccggt gagcaggatg cagctctgca gcagcaggat
     2821 gccaagcgcc gcgccgaagc gcagccgccg cgggcagaag atgagaaagg ggaggccgag
     2881 ctctacgacg aaggtggcgg cggtggcgaa cgccaggagg gcgggtggct gatgcgccgc
     2941 gtaccacgcg agcggtgtcg gcagcggctg ggtgaggaaa tgataagaga gcgccgagag
     3001 gttccaccag ctcgggtctg cgctcagcaa cttcaccgct cccgacatga acatgaagcg
     3061 gaacagcagc cagcgcagca gccagatccc cggggtgatc gtgcagctga gcagcagggc
     3121 gagaaagcca gtctcgagca ggaacgtatc ccactggtag gtcatgaaga cctggccggc
     3181 gtagagcagc gagagataga ggatataaag gaacagcagg ctcaggcggg ggaggaggtt
     3241 cagcgtcagg agcagcgcga gccccgcccc gctccagcat accgcctg
//
LOCUS       DATLKL010000153         1849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_137222, whole genome shotgun sequence.
ACCESSION   DATLKL010000153 DATLKL010000000
VERSION     DATLKL010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137222"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VM713_02045"
     CDS             <1..656
                     /locus_tag="VM713_02045"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HVH83073.1"
                     /translation="DVAASSGVTGICGSGIIEVIAEMYLAGIISQDGVIDGALAARSE
                     RIVPTGRTFAYTVHSGPPLLQVTQNDVRAIQLAKAALRAGVQLLMDRLSVTQVDRIRL
                     ADAFGSHIDVKYAMILGMIPDCELSQVSSAGNAAGTGARIALLDKTSRKTIEDLVHRV
                     EKIETAIEPKFQAHFVEAMGIPHSTEPYPHLRKAVQLPEAKATAPRGSPRGRRRAGG"
     gene            696..1715
                     /locus_tag="VM713_02050"
     CDS             696..1715
                     /locus_tag="VM713_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVH83074.1"
                     /translation="MGTSDLSETGTAAISAALEIRGVTKSFGSTRAIDALDLVIARGR
                     TYGLIGPSGAGKTTAIRMIMSILFPDSGSLRVLGKASAHEARDRIGYLPEERGVYRKM
                     RVNQFLSYIGRLKGGARAEVAARSLRLLGRLGLADAASKKCEELSKGMLQRVQFVAAI
                     INEPELLILDEPFGGLVPVSVRLLKELIADEQRRGATIIFSTHVMAHAEELCQQIVMI
                     HKGRKVLDEPMAGLRRQYDVRTIRLEPLDPGADLTVLRTVVGVESVRAADGSCEIRLA
                     AGTDPGTAMRAIAASVAPARIELARVRLEDVFISLVTGRTSTDEANLALRTHLQGLGT
                     EGATS"
BASE COUNT          304 a          611 c          631 g          303 t
ORIGIN      
        1 tcgatgtggc tgccagcagc ggtgtcaccg gcatctgcgg ctcgggcatc atcgaggtga
       61 tcgccgagat gtacctcgcc ggcatcatca gccaggacgg cgtcatcgac ggcgcgctcg
      121 cggcgcgcag cgagcgcatc gtgccgacgg gacgcacctt cgcctatacg gtgcacagcg
      181 gcccgccgct cctgcaggtg acgcagaacg acgtgcgcgc gattcagctg gcgaaggcgg
      241 cgctccgtgc gggcgtgcag ctgctcatgg atcgcttaag cgtcactcag gtcgaccgta
      301 tccgcctcgc cgacgcgttc ggcagccaca tcgacgtcaa gtacgcgatg atcctcggca
      361 tgattcccga ctgcgagctc tcgcaggtgt cctccgccgg caacgccgcc ggcaccgggg
      421 cacgcatcgc gctgctcgac aaaacctccc gcaagaccat cgaggatctg gtgcatcggg
      481 tcgagaagat cgagacggcg atcgagccga agtttcaggc gcacttcgtc gaggcgatgg
      541 ggattcccca ctcgaccgag ccgtacccgc atctgcgcaa ggcggtgcag ctgccggagg
      601 cgaaggccac ggcgccacgc ggcagtcccc gcgggcggcg ccgcgccgga ggttgactcc
      661 agcgaccatt gacgtgccgc gctaccattc acagcgtggg tacctcagac ctctcagaaa
      721 ccggcaccgc tgcgattagc gcggcactcg agatccgcgg cgttaccaaa agcttcggaa
      781 gcacgcgtgc gatcgatgct ctggacctgg tcatagcgcg cggccgcact tacgggctga
      841 tcggaccgag cggcgccggc aagaccactg cgatccgcat gatcatgtcg atcctgtttc
      901 cggattccgg cagcctgcgg gtgctcggca aggcttcggc gcacgaagcg agggaccgta
      961 tcggttacct gcccgaggag cgcggcgtgt accgcaagat gcgggtcaac cagttccttt
     1021 catacatcgg gcgcctgaaa ggcggcgccc gcgccgaggt ggcggcgcgc tccctacgcc
     1081 tgctcgggcg gctcggactt gccgacgccg cctcgaagaa gtgcgaggag ctctcgaagg
     1141 ggatgctgca gcgcgtgcag ttcgtggcgg cgatcatcaa cgaacccgag ttgctgattc
     1201 tcgacgagcc cttcggtggc ctcgtcccgg tcagcgtccg gctgctgaag gagctgatcg
     1261 cggacgagca gcggcgcggc gcgacgatca tcttctcgac gcacgtgatg gcgcacgccg
     1321 aggagctgtg ccagcagatc gtgatgatcc acaaggggcg caaggtcctc gacgagccga
     1381 tggcgggtct gcgccgccaa tacgatgtgc gcacgattcg gctcgagccg ctcgacccgg
     1441 gcgcggattt gacggtgctg cggacggtgg tcggcgtcga gtccgtgcgc gcggcggatg
     1501 gctcatgcga aatccggctc gcggccggaa ccgatccggg caccgcgatg cgcgcgatcg
     1561 cggcgagcgt tgcgcccgcg cgtatcgagc tggcgcgcgt gcgcctcgag gacgtattca
     1621 tcagcctcgt gacggggcgc acgagtaccg atgaggccaa cctggcactg cgtactcacc
     1681 tccaggggct gggaaccgaa ggggcgacat cgtgagcagt cgagtcctca tggtggcgcg
     1741 gcgcgacttc attgcgacca tcaccagccg gggctttcta ctcggcctgc tgctgatgcc
     1801 cctgttgatg ttcctcgtcg tgctgctgat cccgcgattc gccggtctg
//
LOCUS       DATLKL010000154         1665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_182345, whole genome shotgun sequence.
ACCESSION   DATLKL010000154 DATLKL010000000
VERSION     DATLKL010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182345"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VM713_02055"
     CDS             <1..358
                     /locus_tag="VM713_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVH83075.1"
                     /translation="LRGALEARQAQFDASVGQYNQTLIDAVHDVADVVTNWRALERES
                     AEQQTALAAAQRAYDLTTERYRAGLDNYLTVLSTENQLLLAEAAGAQLAAQRLTFSVD
                     LVRALGGGYTPRAPAG"
     gene            384..1571
                     /locus_tag="VM713_02060"
     CDS             384..1571
                     /locus_tag="VM713_02060"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HVH83076.1"
                     /translation="MRTAPAPVASRLRERRTWLMILAVGLGVTALATLAWWWLYASRY
                     QSTDDAYAAGDLVGVMSQVSGTVVSIGADDTDRVQAGQELVRLDATDYRIALQEAEQQ
                     LARAVRQVHTVFANRDELGALVAQRRADLDRAQADLARRRGLTTSGAVSSEEVGHAQD
                     AVNAARDALTAAEKNLAAGTALVGRTGVPDNPDVRAAATQVERAWLNVQRTSVRAPVS
                     GYVARRSVQLGERIAPGTPLMAIVPLERLRVDANFKEVQLDRMRIGQPARVIADLYGS
                     HVEYRGTVEGLGMGTGAAFALLPAQNATGNWIKVVQRVPVRIALEPQELEQHPLRVGL
                     STRVRVEVSDSSGSQLATAPRREPVLATDAYDTDLSEIRARIAEIIQDNSGKGRAAAL
                     ARD"
BASE COUNT          256 a          585 c          599 g          225 t
ORIGIN      
        1 gctccgtggc gcactcgagg cgcgccaggc gcagttcgac gcgtccgtcg gacaatacaa
       61 ccagaccctc atcgacgcgg tgcacgacgt ggccgatgtg gtgacgaact ggcgggcgct
      121 cgagcgtgag agcgcggagc agcagacggc cctcgccgcc gcgcagcgcg cttatgacct
      181 gacgaccgaa cgctaccgcg ccggactcga caactacctc acggtgctgt cgacggagaa
      241 ccagctgctg ctcgcagagg ccgcgggcgc tcagctcgcg gcgcagcgcc tcaccttcag
      301 cgtggatctg gtgcgggcgc tcggcggggg ttatacgccg cgcgccccgg ccggctaggc
      361 aggccaagac catggatgcc tcggtgagga ctgcgcccgc gcccgttgcg agccgcctgc
      421 gggagcggcg cacctggctc atgattctgg cggtggggct cggcgtcact gcgctcgcca
      481 cgctcgcgtg gtggtggctc tatgcgagcc gctatcagag caccgacgac gcctacgccg
      541 ccggcgatct ggtcggcgtc atgtcgcagg tgagtggcac cgtcgtctcg atcggcgccg
      601 acgacaccga ccgcgtgcag gccggacagg agctggtgcg cctcgacgcc accgattacc
      661 gcatcgcgct ccaggaggcc gagcagcagc tcgcacgcgc cgtgcgccag gtgcacaccg
      721 tgttcgccaa ccgcgacgag ctcggcgcgc tggtcgcgca gcggcgcgcc gacctcgacc
      781 gcgcgcaggc cgatctggcg cgccgccgcg gtctcaccac gagcggtgca gtatccagcg
      841 aggaggtggg tcacgcgcag gatgccgtga acgccgcgcg cgacgcgctc actgcggccg
      901 agaagaacct cgcggccggt accgcgctcg tcgggcgcac cggcgtcccg gacaaccccg
      961 atgtgcgcgc ggccgccact caggtggagc gcgcctggct caacgtgcaa cgtaccagcg
     1021 tgcgcgcgcc ggtgtcgggc tacgtcgcgc ggcgcagcgt gcagctcggc gagcgcatcg
     1081 cccccggaac ccccctcatg gcgatcgtgc cgctcgagcg actgcgggtc gatgccaact
     1141 tcaaggaggt gcagctcgat cgcatgcgca tcgggcagcc ggcgcgtgtc atcgccgacc
     1201 tctacggcag ccacgtcgag taccgcggca cggtcgaggg gctgggaatg ggtaccgggg
     1261 ccgcatttgc gctgctgcct gcccagaacg cgaccggtaa ctggatcaaa gtagtgcagc
     1321 gcgtgccggt gcgcatcgcg ctcgagccgc aggagctcga gcagcacccg ctgcgggtcg
     1381 gactctcgac gcgcgtgcgg gtcgaggtga gtgacagctc cggcagccag ctcgcgaccg
     1441 cgccgcgtcg tgagccggtg ctcgcaacgg acgcctacga cacggatctc agtgagatcc
     1501 gcgcgcgcat cgccgagatc atccaggaca actccgggaa gggccgcgcg gccgcgctcg
     1561 cgcgcgatta gtgcaggcgg cggggcgcgc gcctcattcg gtcttgagat aacacagcgg
     1621 aagcgcggtc gtgtacttga tcgtctcgag cgcgaagctg gcaag
//
LOCUS       DATLKL010000155         1762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_35728, whole genome shotgun sequence.
ACCESSION   DATLKL010000155 DATLKL010000000
VERSION     DATLKL010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35728"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VM713_02065"
     CDS             complement(<1..648)
                     /locus_tag="VM713_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVH83077.1"
                     /translation="MDFFYSIVRSFTAGGVFMFPILFTGALAAAITIERYITLARMRA
                     RNQRTWSRVEPAIASGDFDKARELVSKDESPIAHMLGMGLALQGAVRRREDVEKAMQE
                     SMIEIVPQIEKRTHYLATLANVATLLGLLGTVSGLIHAFAAVATVNPAEKANLLSASI
                     SEAMNCTAFGLMTAVPILVGNAQGRGCLELALIHREDAAADDFRRVRRLVEGQPQD"
     gene            complement(720..1139)
                     /locus_tag="VM713_02070"
     CDS             complement(720..1139)
                     /locus_tag="VM713_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83078.1"
                     /translation="MLRKLILSLAVVTGAGMALADEPSTDATTSSASEKAVTTEAAKE
                     PAQKRTTKAAGIDDKATASAAATAAAQPGGDVKMSGMSILGNEDAPKSLVLVPWKSSK
                     LGDMPSVSRLLDSSKQPVDKEVFLRELSYYEFRAGTK"
     gene            complement(1149..>1762)
                     /locus_tag="VM713_02075"
     CDS             complement(1149..>1762)
                     /locus_tag="VM713_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH83079.1"
                     /translation="LAGCATTTPSTQTTPATKTAPATRTGGAAAAVQADAGGFQLTQK
                     LRVPSDARADYDNAMRLVNAGQYDQGIALLKRVTDKAPAAAAAYVDLGIAYGRSRNLD
                     DAVTSLKRAVELNPRQLVAYNELGMLYRRKGDFSAARESYQKALGIYPGFHYARLNLA
                     ILCDVYLGDVACAQENYAAYQRLVPDDAQVAAWAADLSARAAH"
BASE COUNT          282 a          606 c          553 g          321 t
ORIGIN      
        1 atcctggggc tgaccctcaa ccaggcggcg tactcggcgg aaatcatccg cggcggcatc
       61 ctctcggtgg atcaaggcca gctcgaggca gccgcggccc tgggcattcc ccaccaggat
      121 cggcaccgcg gtcatgagcc cgaaggccgt gcagttcatg gcctccgaga tactcgccga
      181 caggaggttg gccttctcgg ccgggttcac ggtcgccacc gcggcgaacg cgtggatcag
      241 gccgctcacc gtgccgagga gcccgagcag cgtcgcgaca ttggccagcg tcgcgagata
      301 gtgggtgcgc ttctcgatct gcggcacgat ctcgatcatg ctctcctgca tcgccttctc
      361 gacgtcctcg cgccgccgca ccgccccctg cagcgccagc cccatgccga gcatgtgcgc
      421 gatcggggac tcgtccttgc tcacgagctc acgcgccttg tcgaagtcgc cgctcgcgat
      481 cgccggctcg acccggctcc acgtgcgttg attgcgggcg cgcatccgcg cgagggttat
      541 atagcgctcg atcgtgatcg cggcggcgag agcccccgtg aacaggatcg ggaacatgaa
      601 caccccgcct gcggtgaaag acctgacaat tgagtagaag aagtccatat gcatacctct
      661 tgaaacgata cgatcagtca aaccaattca gaatccagtc tcaaatccgg caggcccgct
      721 catttcgtgc ctgctctgaa ctcgtaatag gagagctccc gcaggaacac ctccttgtcc
      781 accggctgct tcgaggagtc gagcagcctc gagacgctcg gcatgtcccc gagcttcgag
      841 ctcttccagg gaaccagcac gagagacttc ggagcgtcct cgtttccgag gatcgacatg
      901 cccgacatct tcacgtcacc gccgggctgc gccgcagcgg tggcagccgc cgacgccgta
      961 gccttgtcgt cgatgcccgc cgctttcgtc gtccgcttct gtgccggctc cttcgccgcc
     1021 tcagtggtca ccgccttctc cgatgcgctg gatgtcgtcg catcggtcga cggttcgtcg
     1081 gcgagagcca tgccggcgcc agtaaccacc gccagtgaca gaatgagttt gcgcaacatg
     1141 cgtccctcct aatgcgcggc tcgcgcgctg agatccgcgg cccacgcggc gacctgggcg
     1201 tcgtccggca ccagccgctg gtaggcggcg tagttctcct gggcgcacgc aacatcaccc
     1261 agatacacat cgcagagaat cgcgagattc agccgtgcgt agtgaaaccc cggatatatt
     1321 cccagggcct tctggtagct ttcgcgcgcg gcgctgaaat cgcccttgcg ccggtagagc
     1381 atgccgagct cgttgtaggc gacgagctgg cgcgggttga gctcgaccgc ccgcttgaga
     1441 ctcgtcaccg cgtcgtcgag attcctggac ctgccgtagg cgatgccgag atcgacgtac
     1501 gccgccgcgg ccgcgggtgc tttgtcggtg acccgcttga ggagcgctat cccctgatcg
     1561 tattgcccgg cgttcaccag gcgcatcgcg ttgtcgtagt cggcccgcgc gtccgagggc
     1621 acgcgcagct tctgcgtgag ctgaaagccg cccgcgtccg cctgcactgc cgccgccgcg
     1681 cccccggtcc tggtcgcagg tgcggtcttg gttgcaggcg tggtctgggt cgaaggcgtg
     1741 gtcgtggcgc aaccggcgag cg
//
LOCUS       DATLKL010000156         2565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_69603, whole genome shotgun sequence.
ACCESSION   DATLKL010000156 DATLKL010000000
VERSION     DATLKL010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69603"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VM713_02080"
     CDS             <1..186
                     /locus_tag="VM713_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVH83080.1"
                     /translation="GATVTWKNTDDGPHTVVSDSGAFRSSAPDTNESFAFRFDKPGTY
                     HYACSIHPRMVGTIVVQ"
     gene            187..711
                     /locus_tag="VM713_02085"
     CDS             187..711
                     /locus_tag="VM713_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVH83081.1"
                     /translation="MLFSRAGELALRGAAALAATLGVGALVVALFIASGVYNIGADDH
                     HTTAVTALIGELRDHSVEVRARAVAVPELGGAARIEAGAHRYALDCAGCHLAPGVNRS
                     ELRRGLYPHPPNLAQQPPPDPRRAFWIIKHGIKMSAMPAWGTTLADPEIWDLVAFLQG
                     VSAMSPEDYQRLTR"
     gene            908..1111
                     /locus_tag="VM713_02090"
     CDS             908..1111
                     /locus_tag="VM713_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83082.1"
                     /translation="MARRPPNDGASWSARELKTLKALAKAGTPTSQVAKKLGRTAAAV
                     QQKAMRCGISFRAAKRSRARRKK"
     gene            1223..1966
                     /locus_tag="VM713_02095"
     CDS             1223..1966
                     /locus_tag="VM713_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83083.1"
                     /translation="MYKLIRLSWIPLALCLSACDVQVNDTTPAQYQANNDIGMYEITA
                     TAKEGTLVTAGSLVLFAIGDSQKVPLSLNSDGSGWYGLYSVRCQSSFPIQILAVWRLQ
                     GLSTRNALTPPQPRMVKLTEPPLTKHEGFDTAPKTGESKAPRGKGGKGGKGGKGEWEG
                     TVNYRFVTVPVTQITFARVEPSSSSAEDVAAARAISVRTPLPLQAACGENAAVRLVST
                     EQRAHGELLIETDNPGLPRWQTYVEFSPK"
     gene            1963..>2565
                     /locus_tag="VM713_02100"
     CDS             1963..>2565
                     /locus_tag="VM713_02100"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HVH83084.1"
                     /translation="MRPGAPPLGVLAAALLACLATVSSAALTVATPAAAPLKIGIIGT
                     GQIGGTLARLWVAAGHEVLMSSRHPDQLKGLAAALGPRARVGTPREAALFGEVVLMSV
                     PYGALPQVGRDLKAELAGKIVLDACNPYPSRDGEMAVEARKVSTGVADPQFLPGVRLV
                     RAFNAINSGDLASEAHRAGEPIAIPLAGDDGQALAVAERLV"
BASE COUNT          416 a          872 c          874 g          403 t
ORIGIN      
        1 ggagcgaccg tgacctggaa gaacacggac gatgggcccc acaccgtcgt aagtgactcg
       61 ggagctttcc gctcgagtgc ccccgatacc aacgagagct tcgcgttcag gttcgacaag
      121 cccggcacct accactatgc gtgctcgatc catccacgca tggtgggcac gatcgtggtc
      181 cagtagatgc tgttcagccg ggcgggggag ttggcactgc ggggcgcggc ggcgcttgcg
      241 gcgacgctcg gagtcggcgc gctcgttgtg gcactgttca tcgcctcggg cgtgtacaac
      301 atcggcgccg atgaccatca caccacggcc gtgaccgctc tcatcgggga gctgcgcgac
      361 cattcggtcg aggtgcgcgc gcgcgccgtc gcggtgccgg agctcggcgg ggcggcgcgc
      421 atcgaggcgg gagcgcaccg ctatgcgctc gattgcgccg gctgtcatct cgcgcccgga
      481 gtcaaccgct cggagctgcg ccgcggactc tatccgcatc cgcccaatct cgctcagcag
      541 ccgccacccg acccgcgccg tgccttctgg atcatcaaac acgggatcaa gatgagcgcc
      601 atgcccgcct ggggcacgac gcttgcggat ccggagatct gggacctggt tgcgttcctc
      661 caaggcgtgt cggccatgag tcccgaggac tatcagcgcc tgacgcgctg accccgcccg
      721 cccccgcagc gacgcatagt cgcgacattg ttacgagttg gtgtgggcgc ggcgcagcaa
      781 caaccgtatg ctgcgccacg gccattccaa catgttacag atcgttcgtc ggacgcgcgc
      841 ttcgccgccc aatcggtgta tgatccgcag ctcggccccg gagttgggta attagcggag
      901 taccgagatg gcaagacgtc ccccgaacga cggcgcttcc tggagcgcgc gtgaactgaa
      961 gaccctcaag gcactcgcga aggcgggcac tcccacgagt caggtcgcga agaagctagg
     1021 gcgcacggcc gcggcggtcc agcagaaggc catgcgctgt ggcatctcct tccgggcggc
     1081 gaagcgctcc cgggcccgga gaaagaagta gggggcgggg cgaccagcct tcaccgcgcg
     1141 ggccgcgctt ggcgagcggc ccggctcagc cttcccagcc gattgcaacg gacctgtctc
     1201 tgttagtcag gaagggggca gcatgtacaa gctcatccgg ctttcgtgga ttccgctcgc
     1261 gctctgcctg agcgcctgcg acgtgcaggt gaacgacaca accccggccc agtaccaggc
     1321 caacaatgac atcggcatgt atgagatcac cgcgaccgcc aaggagggca cgctggtgac
     1381 cgcgggctcg ctggtgttgt ttgctatcgg cgacagtcag aaagtcccgc tcagcctcaa
     1441 ctcggacggc tcaggctggt acggcctgta ctcggtgcgc tgccagagca gctttccgat
     1501 ccagatcctg gcggtatggc gtctgcaggg gctttccacc cgcaacgccc tgacgccccc
     1561 tcagccgcgc atggtgaagc tgaccgagcc acccctcacg aagcacgagg ggttcgacac
     1621 cgcgcccaag acgggggaga gcaaggctcc gcggggcaag ggtggcaagg gtggcaaggg
     1681 tggcaagggt gagtgggagg gcacggtcaa ctaccgtttc gtcaccgtgc ccgtgaccca
     1741 gatcactttc gcccgcgtcg agccctcgag ctcgtcggcc gaggacgtcg ccgcggcgcg
     1801 tgccatctcg gtgaggaccc ccctgccgtt gcaggccgcc tgcggcgaga acgcggcggt
     1861 gcgtctggtc tccacggagc agcgcgccca cggcgagctc ctgatcgaaa ccgacaatcc
     1921 cggcctgccg cgctggcaga cgtacgtgga gttctcaccc aaatgagacc gggcgcaccg
     1981 ccgcttgggg tcctcgcggc tgcgctcctc gcctgcctcg ccaccgttag cagtgctgcg
     2041 ctgacggtcg cgacgccggc cgctgcacct ctcaagatcg gcatcatcgg caccgggcag
     2101 atcggcggca cgctggcgcg gctctgggtt gcggcagggc atgaggtgct catgtcctcc
     2161 cgccatcccg atcagctcaa ggggctggcc gcagcgctcg gacccagagc gcgggtcggc
     2221 acgccacgcg aggccgcgct gttcggcgag gtcgtgctga tgtccgtccc ttacggggcg
     2281 ctgccgcagg tcggtcgcga cctcaaggcg gagctcgccg gcaagatcgt gctcgatgcc
     2341 tgcaacccgt acccgtcgcg tgacggggag atggccgtcg aagcgcgcaa ggtgagtacc
     2401 ggggtcgccg atccgcagtt tctaccgggc gtacgtctgg tgcgcgcgtt caacgccatc
     2461 aattccgggg acctggcgag cgaggcgcat cgcgcgggcg agccgatcgc catccccctc
     2521 gccggtgacg acggccaagc gctcgccgtg gcggagcggc tggtg
//
LOCUS       DATLKL010000157         1708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_12912, whole genome shotgun sequence.
ACCESSION   DATLKL010000157 DATLKL010000000
VERSION     DATLKL010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12912"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1708
                     /locus_tag="VM713_02105"
     CDS             <1..>1708
                     /locus_tag="VM713_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HVH83085.1"
                     /translation="EQLKNALGEQKRSGLKLGRVLIDLGYLSDQAIHDALAKHLQIPF
                     VDVRHLQLAPATVRLLPEVHARRFRAMVLKSDTQGLTVGMSDPTDLLAYDELSARLKR
                     PIKLALVRDADLMKMLDVVYRRTDEIASIAQEVGEELRDGDIDLARLAVDEGSPDAPV
                     IRLIQSIFQDAVQMKASDVHIEPAEAVLRVRQRVDGNLQEQVIDGRRVVSALVSRLKL
                     MSGLDIAEKRLPQDGRFSVKIGGKSIDVRVATMPTQFGESMVLRLLDQSSNLLRLEDL
                     GMPAPLLGRFRALIERSAGMVLVTGPTGSGKTTTLYSALSHLNRTDTKIITVEDPVEY
                     RLDRVNQVQVNQKIGLDFARVLRTTLRQDPDVILIGEMRDHETVDIALRAAITGHLVL
                     STLHTMGAIPTIHRLLDMGAAGYMIAASMQGIVAQRLLRRVCEYCARPATASEYENAW
                     LAAHAGKRSGTAQLKAGAGCTYCNLSGYRGRIAVYELLEFDRTLADAIRRGDLRVLDE
                     AAHARPDFVSLTQSALELALRGVTTLEEAITVTSGLEEEEDLSGVTASWRSPFAAPSG
                     DRL"
BASE COUNT          292 a          580 c          572 g          264 t
ORIGIN      
        1 aggagcagct gaagaacgcc ctcggcgagc agaagcgcag cggactgaag ctcggccgcg
       61 tcctgatcga cctcggctac ctcagcgacc aggcgatcca cgatgcactg gcgaagcacc
      121 tgcagatccc gttcgtggac gttcggcacc tgcagctcgc gcccgccacc gtgcgactgc
      181 ttcccgaggt gcacgcgcgg cgcttccgcg ccatggttct caagtccgac acacagggac
      241 ttaccgtcgg gatgtcggac ccgaccgacc tgctcgccta cgacgagctc agcgcccggc
      301 tgaaacgtcc catcaagctc gcgctggtgc gcgatgccga cctgatgaag atgctggatg
      361 tcgtctatcg ccgcaccgac gagatcgcat cgattgccca ggaggtcggc gaggagctgc
      421 gtgacgggga catcgacctc gcgcgcctcg cagtggacga agggtcgccg gacgcgccgg
      481 tcatccggct catccagagc atcttccagg acgccgtgca aatgaaggcg tccgacgtgc
      541 acatcgagcc ggccgaagcg gtgctgcgtg tgcggcagcg ggtcgacggc aacctgcagg
      601 agcaggtgat cgacggccgc cgggtcgtga gcgcgctggt gtcgcggctc aagctcatga
      661 gcggactcga tatcgccgag aagcgcctgc cgcaagacgg gcgcttctcg gtgaagatcg
      721 gcggcaagtc gatcgacgtg cgcgttgcga ccatgcccac tcagttcggc gagtcgatgg
      781 tgctgcgcct gctggatcag tcctcgaacc tgctgcgtct cgaggatctt ggcatgccgg
      841 cgccgctcct cgggcggttc cgcgcgctca tcgagcgcag cgcaggcatg gtgctcgtca
      901 ccggaccgac cggcagcggc aagaccacga ccctgtattc cgcgctcagc catctcaatc
      961 gcaccgacac caagatcatc acggtcgagg accccgttga gtaccggctc gaccgagtga
     1021 atcaagtgca ggtgaaccag aagatcggcc tggatttcgc gcgcgtactg cgcacgacgc
     1081 tgcgccagga tccagacgtc atcctgatcg gtgagatgcg cgaccacgag acggtcgaca
     1141 tcgcgctgcg ggcggcgatc accggccacc tggtcctctc gacgctgcac accatgggcg
     1201 cgattcccac catccaccgt ctgctcgaca tgggcgcggc cgggtacatg atcgccgctt
     1261 ccatgcaagg gatcgtggcg cagcggctgc tgcgacgggt gtgcgagtac tgcgcccggc
     1321 ccgcgacggc aagcgagtac gagaacgcgt ggcttgccgc acacgccggc aagcgcagcg
     1381 gcaccgcgca gctcaaggcc ggtgccggct gcacctactg caatctttcg ggataccgcg
     1441 ggcggatcgc cgtctacgag ctgctcgagt tcgatcgcac cctggccgac gcgatccgcc
     1501 gcggcgacct gcgcgtgctt gatgaggccg cgcacgcccg gcccgacttt gtctcgctca
     1561 cgcagagcgc gctggagctg gcgctgcggg gtgtcacgac gctggaggag gcgatcacgg
     1621 tcacgagcgg cctcgaggag gaagaggacc tgagcggtgt gaccgcctcg tggcgctcgc
     1681 cgttcgcagc gccctccggc gatcgcct
//
LOCUS       DATLKL010000158         1563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_58210, whole genome shotgun sequence.
ACCESSION   DATLKL010000158 DATLKL010000000
VERSION     DATLKL010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58210"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VM713_02110"
     CDS             complement(<1..374)
                     /locus_tag="VM713_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVH83086.1"
                     /translation="MLDFELLRAFVAVADCGGFRRAAERLNLTQSTVSQQIKRLELET
                     KRPLFRRTTRSVALTDDGEMLLGDARRLLQLEEAARLRLAAPRLSGTVRLGVVEEVAG
                     GSLPSALGRFTALHRGVKLEVQ"
     gene            485..>1563
                     /locus_tag="VM713_02115"
     CDS             485..>1563
                     /locus_tag="VM713_02115"
                     /inference="COORDINATES: protein motif:HMM:NF028267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HVH83087.1"
                     /translation="MSGTSRRGADRASRLARAAALSGTSGKLCPSECATQLLEAIIEP
                     GDRVAIEGDNQKQADFLASALGNVNPGRVHDLHMVQSVLALPDHLAVFERGIANRLDF
                     SYAGPQSRRLAELVASKALTIGAIHTYLELYARWLVDLNPQVALVVADKADCDGNLYT
                     GPNTEDTPTITEAAAFRGGIVVAQVNEIVDHLPRVDVPGDWVDFVIPSPKPYLIDPLF
                     TRDPAKIRNENVLMAMMAIAAVYEPYEVRRLNHGIGYATAAIELTLPTFAAQRGLKGK
                     IARHFVLNPHPTLIPAIEAGFVDSVYCFGSEIGMERYASRHADIFPVGSDGNLRSNRA
                     LAQVAGQYACDLFIGGTLQIDAPGN"
BASE COUNT          297 a          528 c          470 g          268 t
ORIGIN      
        1 atctgcacct cgagcttcac gcctcgatga agcgcggtga agcgaccgag cgccgaaggc
       61 agcgagcccc cggcaacctc ctcgaccacg ccgagacgca ccgtgccgga caggcgcggc
      121 gccgccaggc gcagacgggc agcttcctcg agctgcagca ggcgacgcgc gtcgccgagc
      181 aacatctccc cgtcatccgt cagcgcaacg ctgcgtgtcg tgcgccggaa caagggacgc
      241 ttggtctcga gctcgagccg cttgatctgc tggctcaccg tcgactgggt caggttgagc
      301 cgctcggccg cccggcggaa gccgccgcaa tcggccacgg caacgaaggc gcgcaacagc
      361 tcgaaatcca gcatgcaatt gatcctcttt ttcgatcaat catattataa aatatcgttt
      421 cgataatcaa tgatgacctc ctatctgccg cttcaagggc aggattgagc gaaggaggtc
      481 catcatgtca ggcacgtcac gacgcggagc agatcgcgcc tcccgtctcg cacgggctgc
      541 cgcgctcagc ggcacaagcg gaaagctctg cccgtccgag tgtgcgaccc agttgctcga
      601 agcgatcatc gagcccggcg accgcgtggc gatcgagggc gacaatcaga agcaggccga
      661 ttttcttgcc tccgccttgg gaaatgtgaa tccggggcgg gtccacgatc tgcacatggt
      721 gcaatcggtc ctggcgctgc ccgaccacct cgccgtgttc gaacgcggca tcgcgaaccg
      781 gctggatttc tcgtacgccg gaccacaaag ccgcaggctc gccgagctcg ttgcgtcgaa
      841 ggccctcacg atcggtgcga tccacaccta tctcgaactc tatgcgcgct ggctggtgga
      901 tctgaaccca caggtggcgc tcgtcgtcgc tgacaaggct gactgcgacg gtaatctcta
      961 taccggtccg aacaccgagg atacgcccac gatcaccgag gctgcggcgt tccgcggcgg
     1021 catcgtcgtc gcgcaggtca acgagatcgt ggaccacctg ccgcgggtcg acgtacccgg
     1081 cgattgggtc gatttcgtca taccgagccc gaagccttac ctgatcgatc cgctgttcac
     1141 gcgcgatccc gccaagatca ggaacgagaa cgtgctgatg gcgatgatgg cgatcgccgc
     1201 tgtctacgag ccctacgagg tgcggcggct caaccacggc atcggctatg ccaccgcagc
     1261 gatcgagctg accctaccga ccttcgccgc ccagcgcgga ctgaaaggca agatcgcccg
     1321 gcatttcgtg ctgaacccgc atccgaccct gatacccgcg atcgaagccg gcttcgtcga
     1381 cagcgtctat tgcttcggct ccgagatcgg catggagcgc tacgcttcgc gccacgcgga
     1441 catttttccg gtcggctccg acggcaatct gcgttcgaac cgcgcgcttg cgcaggtggc
     1501 cggacaatac gcctgcgacc tcttcatcgg cggcacattg cagatcgacg cgccgggcaa
     1561 cag
//
LOCUS       DATLKL010000159         1502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_80783, whole genome shotgun sequence.
ACCESSION   DATLKL010000159 DATLKL010000000
VERSION     DATLKL010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80783"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1502
                     /locus_tag="VM713_02120"
     CDS             <1..>1502
                     /locus_tag="VM713_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83088.1"
                     /translation="GLPSWFQIDRVTIHNGTVVYWNDATGQAETLSRIEGGLSAVTPL
                     GPYRAQGRAVARGVPVAFEATLERFAGATPAALGFKLALTGAELGFTGEGALVDGGTE
                     LAGKLRLSGVDLRATAAAMGADDAEGLPPFLAQNFGLEGTLSASAGKVALNALTLRLG
                     DSEASGAFLFEPGAPNRASLTLELARIDLDKWVAMKNPAPAVGGAPPGGAPPTKPGAA
                     KATGFGLPRDLDATADAKLGALVYKGGVVRQVHFGATLHKGELVLQQLTAALPGGSDV
                     TLYGALRGSGDQPSFVGNVEASSDNLRALFDWLKIDLGKVPAERLRKLTLTAGLQLDP
                     HQLTLHDVDATLDSSHLVGGANILLRGRPAFGAAISIDRLNLDAYLPALAQAKAAAPA
                     EEAAAAAIAGTNPAAASAADAASQPLQFLTTFDANIRARVDELIYRSQGIRGIDLDGN
                     VESGTLTLRKARVEDLAGLKGQVSGSIAAAPGQPKVDMTFDASAADLAPF"
BASE COUNT          222 a          558 c          492 g          230 t
ORIGIN      
        1 gggtttgccg agctggttcc agatcgaccg cgtcaccatt cacaacggca ccgtcgtcta
       61 ttggaatgac gcaacgggac aggccgagac gctgtcccgg atcgagggcg ggctgagcgc
      121 ggtgaccccg ctcggcccat accgcgccca ggggcgcgcc gtggcccgcg gcgtgcctgt
      181 cgcgttcgag gcgacgctcg agcgcttcgc cggtgccacg cccgccgcgc tcggcttcaa
      241 gctcgcgctc accggcgccg agctcggctt caccggcgaa ggcgctcttg tcgatggcgg
      301 gaccgagctc gccggcaagc tgcggctctc cggggttgac ctgcgcgcga ccgccgcagc
      361 gatgggcgcc gatgacgccg aggggctgcc gcccttcctt gcccagaact tcgggctcga
      421 gggcacgctc agcgcgtctg ccgggaaggt cgccttgaac gcgctcaccc tccggctcgg
      481 cgacagcgag gccagcggcg ccttcctctt cgagccgggt gctcccaacc gcgccagcct
      541 caccctcgaa ttggcgcgta tcgatctcga taagtgggtg gcaatgaaga acccggcccc
      601 cgccgtcggc ggcgctccgc ctggcggggc gcccccgacc aagccgggag ccgccaaggc
      661 aaccggattt gggctgccgc gcgatctcga cgccactgcc gatgcaaagc tcggggcgct
      721 ggtctacaag ggtggggtcg tgcgccaggt ccacttcggc gccaccttgc ataagggcga
      781 gctcgtcctg cagcagctca cggccgcgct ccccggcggg tcggatgtga ccctctacgg
      841 cgccctccgc ggctcgggcg atcagccgag tttcgtcggc aacgtggagg cgtcctcgga
      901 caatctgcgc gccctcttcg actggctcaa gatcgacctc ggcaaggttc cggcggagcg
      961 gctacgcaag ctcaccctca ccgccggcct ccagctcgat ccgcaccagc tcacgctgca
     1021 cgatgtcgat gccacgctcg attcctcgca cctagtcggc ggcgccaata ttctgctccg
     1081 ggggcggcca gccttcggcg ccgctatctc gatcgaccgc ctcaatctgg acgcctatct
     1141 gcccgcgctc gcccaagcca aggcggcggc gcccgccgag gaagcggcgg ccgctgctat
     1201 tgcgggaacc aaccccgcag ccgcaagcgc ggccgacgcg gcatcgcagc cgctccagtt
     1261 tctgacgaca ttcgatgcca atattcgcgc gcgggtggac gagctcatct atcgcagcca
     1321 gggcattcgc ggcatcgatc tcgacggtaa tgtcgagtcc ggtacgctga ccttgcggaa
     1381 ggcgcgcgtc gaagatctgg ccggcctcaa gggccaggtc agcggcagca tcgccgccgc
     1441 tcccggtcag cccaaagtgg acatgacctt cgacgcgagc gccgccgacc tcgcgccctt
     1501 cc
//
LOCUS       DATLKL010000160         1578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_204672, whole genome shotgun sequence.
ACCESSION   DATLKL010000160 DATLKL010000000
VERSION     DATLKL010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204672"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VM713_02125"
     CDS             complement(<1..250)
                     /locus_tag="VM713_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83089.1"
                     /translation="MRNLLLSGLLLCAAAPATAQVGLPPVRLPSLPAVNAPALPGVGL
                     PPLTGEAAAALGELDSRSLSELRQRRIRELLRRHRDVLE"
     gene            339..836
                     /locus_tag="VM713_02130"
     CDS             339..836
                     /locus_tag="VM713_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVH83090.1"
                     /translation="MVAELRTELVELLPRLRRFARALARDPHDADDLVQTAIERALAA
                     AGQLRADAPVAGWVFGILRHAWIDEQRARGRRGRLFATGESAEEVADPARGAPLDSIT
                     VQDAVARLPEEQRLAVALVLVEGLSYKEAAHVMEVPIGTLTSRLARARDALQAMLGER
                     DGAGG"
     gene            833..1576
                     /locus_tag="VM713_02135"
     CDS             833..1576
                     /locus_tag="VM713_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83091.1"
                     /translation="MTFSDETVSAYADGELDAATSTALEAAMAADPQLARRVARHRAL
                     RARVQDAFAPVLTEPVPERLLAAARGGASGKRADNVIAFQARLRPRWSWPEWGAMAAC
                     LVAGVLLGPQLLRPSAAPAPLETSGGRVVAGGVLAQALSQQLSSTQPAGAAVEIGVSF
                     RARDGGYCRTFVLREAQSLVGLACRAGTAWQVVTLAQSGTPGAPGGGYRQAGSALPPA
                     VARTLDDLIAGEPLDAAGEAAARARAWNP"
BASE COUNT          215 a          551 c          605 g          207 t
ORIGIN      
        1 cctcgaggac gtcgcgatgc cggcgcagca gctcgcggat gcgccgttgg cgcagctcgc
       61 tcaggctgcg cgagtcgagc tcgccgagcg ccgcggcggc ctcgcccgtc agtggtggga
      121 ggccgacccc cggcaacgcc ggcgcgttca cggccggcag gctcggcaga cgtacgggcg
      181 gcaggcccac ctgtgcggtg gcgggagccg ccgcacacaa caacagtccg gatagcaaca
      241 gattacgcac ggccgcacct taatggaacg tagctgcgcg gggaacaaat ggaagaaaat
      301 cgccagcatg agcgtattgc cggtatgggg tgcccgcgat ggtggctgag ctgcgcacgg
      361 agctggtcga gctgctgccg cggctgcgcc gttttgcgcg tgcgctcgcg agagatcctc
      421 acgatgccga cgacctggtg cagaccgcca tcgagcgcgc gctcgccgct gcgggccagt
      481 tacgcgccga cgcgccggtc gccggctggg tgttcggcat cctgcgccac gcctggatcg
      541 acgagcagcg cgcacgcggc cgccgcgggc ggctgttcgc cacgggtgaa tcggccgagg
      601 aggtcgcgga ccccgcgcgc ggggcaccgc tcgattccat cacggttcag gacgccgtgg
      661 cacgcctccc ggaggagcaa cgtctggcgg tggcactggt gctggtggag ggactctcgt
      721 acaaggaagc ggcgcacgtg atggaggtac cgatcggaac actgacgagc cgcctggcgc
      781 gggcccgcga cgcgctgcag gcgatgctcg gcgagcgcga cggagcaggt ggatgacttt
      841 tagcgacgag actgtcagcg cgtacgcgga cggcgagctg gatgccgcga cgagcacggc
      901 gctcgaggcc gcgatggccg ccgacccgca gctcgcgcgg cgcgtcgcgc gtcaccgcgc
      961 gctgcgcgca cgggtgcagg acgcgtttgc acccgtgctc acggagccag tgcccgagcg
     1021 actgctcgcc gccgcccgcg gcggtgcatc cgggaagcgc gccgacaacg tgattgcatt
     1081 ccaggctcgg ctcaggccgc gctggtcgtg gcccgagtgg ggcgccatgg ccgcctgcct
     1141 cgttgcgggt gtgctgctcg ggccgcagct gttgcggccc tcggccgcac cggccccgct
     1201 cgagacgagc ggcggccggg tcgtggcggg cggcgtgctc gcacaagcgc tctcgcagca
     1261 actctcgagc acccagcccg cgggcgcggc ggtggagatc ggcgtcagct tccgtgccag
     1321 ggatggcggc tactgccgca ctttcgtgct gcgcgaggcg cagtcgctcg tcggactcgc
     1381 gtgccgcgcg ggcacggcct ggcaggtggt aacgctcgcc cagagcggaa cgccgggcgc
     1441 gccgggcggc ggctaccgtc aggcaggatc cgccctgcct cccgccgtgg cgcgcaccct
     1501 cgatgatctg atcgcgggcg agccgctcga cgccgccggc gaggccgccg cgcgcgcgcg
     1561 cgcctggaat ccgtgagg
//
LOCUS       DATLKL010000161         1998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_92170, whole genome shotgun sequence.
ACCESSION   DATLKL010000161 DATLKL010000000
VERSION     DATLKL010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92170"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="VM713_02140"
     CDS             complement(<1..1741)
                     /locus_tag="VM713_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HVH83092.1"
                     /translation="MDLTPILETLNDAQRAAVTAPLGPVLVLAGAGSGKTRVLTHRIA
                     WVIQAEGASPQGILAVTFTNKAAGEMRARVERLLGIPAAALWIGTFHGIAHRLLRIHW
                     REAGLPQGFQIMDSEDQQRLIKKVIRAAELDETRWVPREVQWFINANKDEGRRPGALA
                     DGGDPTRRELIRIYAAYEEACARTGMVDFAELLLRAFELWRDNPQLLGHYRRRFRHVL
                     VDEFQDTNGIQYAWLKLLTGGDGGAPFVVGDDDQCFVAGTPVTMADGSTRPIEEISPG
                     DLVMSCYGSGDLRPARVTEAIGRVRTGHMLTLHLRSGRTLCSTPEHTHFAGYLLGETP
                     QTYFLYLMHKEGIGWRLGTSQVYTKGQEKPMVGFKQRSIQEHADATWIIRTHESENEA
                     RLDEVLTSLRYGLPTLPFTARKGGSAKGLVHDPAYIARVFGSLDTDTAALRLLEDVGL
                     DPDEPHHWPRGRDSIRHNVVLTLCGDRRGKTPMHRISVAGTSPKARGILESLGLSVRP
                     AGHNQRSWRFDTVCKNFGDVMTIARCVREQLDANCRLQGLMYRRALPFVTASAIRPGM
                     VVATDDRSFDIVER"
     gene            complement(1775..>1998)
                     /locus_tag="VM713_02145"
     CDS             complement(1775..>1998)
                     /locus_tag="VM713_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVH83093.1"
                     /translation="FIALWTVGMWVAENVLRPILTSKHAEVSTLAIFVGAIGGVSAFG
                     ILGLILGPVLLSFAVALLRFAEETLAAEP"
BASE COUNT          336 a          660 c          667 g          335 t
ORIGIN      
        1 cccgctccac tatgtcgaag ctccggtcat ccgtcgcgac caccatgccg ggtcgaatgg
       61 ccgatgcggt tacgaatggc aaggcccgcc ggtacatgag cccttgcagt ctgcagttgg
      121 cgtcgagctg ctcccggacg cacctggcaa tcgtcatcac gtcaccgaag ttcttgcaca
      181 cagtatcgaa tcgccagctt cgctggttgt gcccggcggg tcgcacgctc agcccaaggg
      241 actctaggat cccgcgggcc ttaggcgagg tcccggcaac tgagatcctg tgcatcgggg
      301 tcttgccgcg ccggtcgccg cacagcgtga gaacaacgtt atggcggatc gaatctcggc
      361 cccgcggcca gtgatggggt tcatcggggt ccagcccgac gtcctcgaga agccgcagag
      421 cagcggtgtc cgtgtcgagt gagccgaata cccgcgcgat gtaagccgga tcatgcacca
      481 gacccttggc actcccgccc ttgcgcgccg taaacggcag agtcggcagg ccgtacctga
      541 gagacgtcag cacctcgtcg agtcgcgctt cgttctcgct ctcatgggta cggatgatcc
      601 aggtggcgtc ggcgtgttcc tgtatcgagc gctgtttgaa gcccaccatg ggcttctcct
      661 ggcccttggt gtagacctgc gacgtgccga gccgccagcc gatgccttcc ttgtgcatga
      721 ggtacaggaa gtatgtctgc ggcgtctcgc ccaataggta tccggcgaaa tgagtatgct
      781 caggcgtgct gcacagagtc cgccccgagc gtagatgcag ggtcagcatg tgtccggtac
      841 gaacgcggcc gattgcctcg gtgacgcgcg cgggccgcag gtcgccgctg ccatagcagg
      901 acatcaccag atcgccgggc gagatttcct cgatcggacg cgtggagccg tctgccatcg
      961 tgaccggggt gcctgcgaca aagcactgat cgtcatcgcc tacaacgaac ggtgcgccgc
     1021 cgtcgccccc cgtcagcagc ttcagccacg cgtactggat accgttggtg tcctggaatt
     1081 catcgaccag cacgtgcctg aatcgccgtc ggtaatgtcc gaggagctgc ggattgtcgc
     1141 gccacagctc gaaggcgcgc agcagcagct cggcgaagtc gaccatgccg gtgcgcgcac
     1201 acgcctcctc gtaggcggcg tagatccgga tcagctcgcg gcgcgtcgga tcgccgccat
     1261 cggcgagcgc gcccgggcgg cgcccctcgt ccttgttggc attgatgaac cactgcacct
     1321 cgcgcggcac ccagcgtgtc tcgtcgagct cggcggcgcg gattaccttc ttgatgaggc
     1381 gctgctggtc ctcggagtcc atgatctgga agccctgcgg caggccggcc tcgcgccagt
     1441 ggatgcgcag cagccggtgc gcgatgccgt gaaaggtgcc gatccagagc gccgcagcgg
     1501 gaatgcccag gagccgctcg acgcgcgcgc gcatctcgcc ggcggccttg ttggtgaagg
     1561 tgacggcgag gatgccctgt ggggaggcgc cctcggcctg gatcacccag gcgatgcggt
     1621 gcgtcagcac gcgcgtcttg ccgctgccgg cgccggccag caccagcacc ggcccgagcg
     1681 gggcggtgac tgcggcgcgc tgcgcgtcgt tcagcgtttc gaggatcggg gtgagatcca
     1741 tagggcgcgc attctacggg aaaaaggcgc gcgctcaggg ctccgcggcg agggtctcct
     1801 cggcgaagcg cagcagcgcc acggcaaagc tcaacagcac cggaccgagg atcagtccga
     1861 ggatcccgaa cgccgacaca ccgccgatcg cgccgacgaa gatcgcgagc gtcgacacct
     1921 cggcgtgctt ggaagtgagg atcggccgca gcacgttctc ggcgacccac atgccgaccg
     1981 tccagagcgc gatgaaga
//
LOCUS       DATLKL010000162         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_80811, whole genome shotgun sequence.
ACCESSION   DATLKL010000162 DATLKL010000000
VERSION     DATLKL010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80811"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VM713_02150"
     CDS             complement(<1..323)
                     /locus_tag="VM713_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HVH83094.1"
                     /translation="MALRNTTRRWGVIAQTFHWVVVALIIVQVTLAEMADDLPNGLRK
                     LTLLARHKSVGITILALVVLRLAWRWLNEHPPLPDTLKRYELALARFTHTALYVLLFA
                     MPLSG"
     gene            322..1077
                     /locus_tag="VM713_02155"
     CDS             322..1077
                     /locus_tag="VM713_02155"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HVH83095.1"
                     /translation="MELKLKGVHSGCKSGKLGALFLTCLLAACPTRPPPPPAAVRAAP
                     PPPIAPHVGRPYDIVAAQSLLTVLVYRGGPLASAGHNHVIASRTLAGTIYMPGDALKS
                     SFEVHLAVAELTVDAPELRAAEARSDFPPDVPQSARDGTRRNMLGPALLDADNYPEVV
                     LRAVRLERATGDGPPRVTAEMDAQVRDQHRALSVPVAYQLEGDTVTVTGETALRQSEL
                     GLTPFTALLGALAVQDEMHVRFRIVARAAGGAL"
     gene            complement(1084..>2690)
                     /locus_tag="VM713_02160"
     CDS             complement(1084..>2690)
                     /locus_tag="VM713_02160"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HVH83096.1"
                     /translation="SHHATGGPLKCESVTLYQGGQYWLYKYHRYDDVRLVFAPERAIA
                     AFGGDPDNFQFPRWCLDMSVLRAYGPDGKPARTPNFLTLKPAGPDAGEVVFVAGHPGR
                     TERLLTVAQLLELRDGFLPPWLLRASELRGRLIEFGKTGAEASRISEDLLNGLENSIK
                     VRRGQLAALLDERLLAAKRAEETALRERVAADPQLAAATGDPWAEIAKAEAVDRELEL
                     PYTWIELGAGFNSDLFRYARTLLRGAAERAKPNTERLREYRDAALPRVAQRLGAPLPV
                     YPQLEKLTLSFSLERMREWLGPDAPIVRQLLAKDSPDSLAARLVEGSKLADAALRKQL
                     WEGGQAAVAASHDPMIELARAIDAQARAVRKRYEDEVEAPVDAAAEKIARARFKVLGT
                     SEPPDATFTLRLNVGTVQGWTEGSATVEPFTHSARLFERATGADPFRIPDSWLAVQSQ
                     LDPATRFDLSSNNDIIGGNSGSPMIDAHGRVVGLIFDGNIHSISGDYWYDAELNRSVA
                     VDAAIIFEALRRVYQAKELLAEMGTK"
BASE COUNT          400 a          935 c          945 g          410 t
ORIGIN      
        1 cagcccgaca gcggcatcgc gaacagcagc acgtagagcg ctgtgtgggt gaagcgcgcc
       61 agcgcaagct cgtagcgctt gagggtgtcc ggcagcggcg gatgctcgtt cagccagcgc
      121 cacgcgaggc gcaggaccac cagagcgagg atcgtgatgc cgaccgactt gtggcgcgcg
      181 agcagtgtga gcttgcgcag cccgttcggc agatcgtcgg ccatctccgc gagcgtcacc
      241 tgcacgatga tcagtgccac gacgacccag tggaacgtct gggcgattac accccagcgg
      301 cgggtggtgt tacggagggc catggagctt aagcttaaag gggtgcacag cggttgcaag
      361 agcgggaagc tgggtgcgct gtttctgacg tgcctgctcg cggcctgtcc gacgcgccca
      421 ccgccgccgc cggctgcggt gcgcgccgcc ccgccgccgc ccatcgcgcc gcacgtcggc
      481 cgtccctacg acatcgtcgc cgcgcagtcg ctcctcaccg tgctggtgta tcgcggtggg
      541 ccgcttgcct ccgcgggcca caatcacgtc atcgcctccc gcacgctcgc cggcaccatc
      601 tacatgccgg gcgatgcgct caagagcagc ttcgaggtgc acctggcggt ggctgagctg
      661 accgtggacg cgccggagct gcgtgccgcg gaagcccgga gcgacttccc gcccgacgtg
      721 ccgcagtcgg cgcgcgacgg cacgcggcgc aacatgctcg ggcccgcgct gctcgatgcc
      781 gacaactacc cggaagtggt gctgcgcgcc gtgcggctcg agcgggccac gggcgacggg
      841 ccgccccggg tcacggcgga aatggacgca caggtccgtg accagcaccg cgcgctcagc
      901 gttccggtcg cctatcagct cgagggcgat accgttaccg taacggggga gaccgcgctc
      961 cggcagagcg agctcggact cactccgttc acggcgctcc tgggtgcgct cgcggtgcag
     1021 gacgagatgc acgttcgctt ccgcatcgtg gcgcgtgccg cgggcggcgc gctctgacgg
     1081 gtgctacttc gtgcccatct ccgccagcag ctcttttgcc tggtataccc tgcgcagcgc
     1141 ctcgaagatg atcgccgcat ccaccgcgac actgcgattg agctcggcgt cgtaccagta
     1201 gtctccggag atcgagtgga tgttgccgtc gaagatgaga ccgacgacgc gcccgtgggc
     1261 gtcgatcatc ggactccccg aattgccgcc gatgatgtcg ttgttgctcg agaggtcaaa
     1321 gcgcgtcgcc gggtcgagct gactctgcac cgcgagccag ctgtccggga tgcggaaggg
     1381 gtccgccccc gtggcgcgct cgaacagccg cgcgctgtgc gtgaacggct ccaccgtggc
     1441 gctcccctcc gtccaaccct gcacggtgcc gacgttgagg cgcagcgtga aggtcgcatc
     1501 gggcggctcg ctcgtcccga gcaccttgaa gcgcgcccgt gcgatcttct ccgcggccgc
     1561 atcgaccggc gcctccacct catcctcata gcgcttgcgc accgcgcgcg cttgcgcgtc
     1621 gatggcgcgg gcgagctcga tcatcggatc gtgggaggcc gctaccgccg cctggccccc
     1681 ttcccagagc tgcttgcgga gcgctgcgtc ggcgagcttc gagccctcga cgagccgcgc
     1741 ggccaggctg tcgggtgaat ccttcgcgag cagctgccgt acgatcggcg cgtcgggccc
     1801 gagccactcg cgcatgcgct cgagagagaa ggacagggtg agcttctcga gctgcgggta
     1861 aacgggcaag ggggcgccca ggcgctgcgc gacgcgcggc agcgccgcgt cgcggtactc
     1921 gcgcagacgc tcggtgttgg gcttggcgcg ctcggcggcg ccgcgcagca gcgtgcgcgc
     1981 gtagcgaaag agatcgctgt tgaagccggc accgagctcg atccaggtat aggggagctc
     2041 gagctcgcga tccacggctt cggccttggc aatctccgcc cacgggtcgc cggtcgcggc
     2101 cgcgagctgc ggatcagccg ccacccgctc gcgcagcgcc gtctcctcgg cgcgcttggc
     2161 ggcgagcagg cgctcgtcga gcagcgcagc gagctgaccg cggcgcacct tgatggagtt
     2221 ctccagcccg ttcagcaggt cttccgaaat gcggctcgcc tcggcgccgg tcttgccgaa
     2281 ctcgatcagc cgcccgcgca gctccgaggc gcgcagcaac cagggcggca ggaacccatc
     2341 gcggagctcg aggagctggg cgacggtgag caaccgctcg gtacgtcccg gatgccccgc
     2401 cacgaagacg acttcgccgg catccggccc cgcgggcttg agcgtgagga aattcggcgt
     2461 gcgggcgggc ttgccatccg gcccataggc gcgcagtacc gacatgtcga ggcaccagcg
     2521 cgggaactgg aaattgtcgg ggtcgccgcc gaaggcggcg atggcgcgct cgggtgcaaa
     2581 caccagccgc acgtcgtcat agcggtggta cttgtagagc cagtactgcc cgccctggta
     2641 aagagtgacg ctctcgcact tgagcgggcc accggtcgca tggtggctct
//
LOCUS       DATLKL010000163         1539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_126003, whole genome shotgun sequence.
ACCESSION   DATLKL010000163 DATLKL010000000
VERSION     DATLKL010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126003"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1539)
                     /locus_tag="VM713_02165"
     CDS             complement(<1..>1539)
                     /locus_tag="VM713_02165"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HVH83097.1"
                     /translation="DTDDGTGLHHMVFEVVDNSIDESLAGHCDRIVVTIHADESVTVS
                     DNGRGIPVDIHPEEKHSAAEVIMTVLHSGGKFDDSSYKVSGGLHGVGVSVVNALSDYL
                     LLTVARDGKLYRQEYHLGEPAAPLAEVGATGERGTVIRFKPSSRIFTNIQFNYEILAK
                     RLRELSFLNSGVRIELIDERENKSDVFAHAGGLQAFVKYLNRTRTPIHESVFWFRTQD
                     GPVSVEVALQWNDSYQESMFTYTNNIPQKDGGTHLAGFRSALTRTLNDYVEKELSGKK
                     DKIPMTGDDAREGLTAILSVKLPDPKFSSQTKDKLVSSEVKGIVENAVGTKLQEFLLE
                     HPQEARLIVAKMVEAARAREAARKAREMTRRKGALDVAGLPGKLADCQEKDPALSEIF
                     IVEGDSAGGSAKQGRDRRTQAILPLKGKILNVERARFDKMLSSAEVGTLITALGTGIG
                     RDDFDVEKLRYHRICLMTDADVDGSHIRTLLLTFFFRQMPELIENGHIFIAQPPLYKV
                     TRGKS"
BASE COUNT          253 a          481 c          512 g          293 t
ORIGIN      
        1 ggacttgccg cgcgtcacct tgtagagcgg cggctgggcg atgaaaatgt ggccgttctc
       61 gatcagctcc ggcatctggc ggaagaagaa cgtgagcaac aatgttcgga tgtgcgagcc
      121 gtcgacgtcg gcgtcggtca tgagacagat gcggtgatag cgcagcttct cgacgtcgaa
      181 gtcatcgcgt ccgatgcccg tgccgagcgc cgtgatgagc gtgccgacct cggccgagga
      241 cagcatcttg tcgaagcgcg cgcgctcgac gttcaggatc ttgcccttca gaggcaggat
      301 cgcctgggtg cgccggtcgc gcccctgctt ggcggacccg ccggcggaat cgccctcgac
      361 gatgaagatc tcggagagcg ccggatcctt ctcctgacag tcggcgagct tgccgggcag
      421 cccggctaca tcgagcgcgc ctttgcggcg ggtcatctca cgcgccttgc gcgccgcctc
      481 ccgggcacgc gccgcctcca ccatcttcgc gacgatgaga cgcgcctcct gaggatgctc
      541 gaggaggaac tcctggagct tggtgccgac cgcgttctcg acgatgccct tcacctcgga
      601 ggacacgagc ttgtccttgg tctgtgacga gaacttggga tcggggagct tcaccgacag
      661 gatcgcagtc agcccctcgc gcgcgtcgtc gccggtcatc gggatcttgt ctttcttccc
      721 ggagagctcc ttttcgacgt agtcgttgag cgtgcgcgtc agcgcgctgc gaaagccggc
      781 gaggtgcgtt ccgccgtcct tctgcggaat gttgttcgtg taggtgaaca tcgactcctg
      841 ataggagtcg ttccactgga gcgccacctc gaccgacacc ggcccgtcct gcgtgcgaaa
      901 ccagaacacc gattcgtgaa taggcgtgcg cgtacggttg aggtacttca cgaaggcctg
      961 gagtcccccg gcgtgcgcga acacgtcgga cttgttctcc cgctcgtcga tgagctcgat
     1021 ccgcacgccg gaattgagga acgagagctc gcgcagccgc ttggcgagga tctcgtagtt
     1081 gaactggatg ttggtgaaga tccggctgct cggcttgaag cggatcaccg tgccgcgctc
     1141 gcccgtggcg ccgacctcgg ccagcggcgc cgcgggctcg cccaggtggt attcctggcg
     1201 atagagcttg ccgtcgcggg cgaccgtgag cagcaggtag tccgagagcg cgttgacgac
     1261 ggagacgccg acgccgtgca ggccgccgga gactttgtag gagctgtcgt cgaacttccc
     1321 gcccgagtgc agcaccgtca tgatgacctc ggccgccgag tgcttctcct cagggtggat
     1381 atccacaggg atgccgcgac cgttatcgga cacggtgacc gactcatcgg cgtgaatcgt
     1441 gacgactatg cgatcgcagt ggccggccag ggactcgtcg atggagttat cgacgacctc
     1501 gaaaaccatg tggtgaagcc cggtgccatc gtcggtgtc
//
LOCUS       DATLKL010000164         1633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_216051, whole genome shotgun sequence.
ACCESSION   DATLKL010000164 DATLKL010000000
VERSION     DATLKL010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216051"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..>1633
                     /locus_tag="VM713_02170"
     CDS             64..>1633
                     /locus_tag="VM713_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVH83098.1"
                     /translation="MIIRRFTGAAPRALRPSIFLSGWLVAAPAFAADADITAGVNAPA
                     ADEASADSSGQLSTIVVTARRLNVARTEIETQTGASTYKITSDAIAATPGGENVQMNQ
                     VLLQAPDVVQDSFGQLHVRGDHNDLQYRLNGIILPEGISVFSQTLSPRLISSLSLITG
                     ALPAEYGLRTAGIVDLTTNSGLLQPGGSVSLYGGSFGTFEPAVEYGGNTGHLSYYVSF
                     DYKQDKVGIESPDGSANPLHDHTKQLHGFGYFENIVDENNRLSLILGTSDDAFQIPNQ
                     RGLQPGLGLTVNGQTTFLSDDLNETQHEAAQYAIVSWQHAAGALNWQTSLSARYTTLH
                     FAPDLLGDLLYNGIAQDAYKSDTAFGWQTDGSYKWNDAHTVRAGFYIQHDSAKSNTTS
                     RVLPLDPVTGVPTTDVPEAIVDNGSQSQSLESVYLQDEWQVLKPLTINYGLRFDHYSA
                     YSNGSQLSPRVNFVWELAEGTTVHGGYSRYFTPPPFELIGSPTLAKFLNTTALPPGSV
                     TLDDSPIAARANYYD"
BASE COUNT          303 a          583 c          484 g          263 t
ORIGIN      
        1 tgaaggcgca gcccgattca catcggggtg cgcgacgttt ctggctcttg tggagtttct
       61 ttcatgatca ttcgtcgatt cacgggcgcc gcgccccgcg cgctgcggcc gagcatcttc
      121 cttagcggct ggctcgtcgc cgcgccggcg ttcgccgccg atgccgatat caccgccggc
      181 gtcaatgcac ccgccgccga cgaggcttcc gccgacagca gcggccagct ctcgaccatc
      241 gtcgtcaccg cgcggcggct caacgtcgcg cgcaccgaga tcgagacgca gaccggcgcc
      301 tcgacttaca agatcacgag cgatgcaatc gccgcgaccc cgggtggcga gaacgtgcag
      361 atgaaccagg tgctgctgca ggcgccggac gtggtgcagg actccttcgg gcagctgcac
      421 gtgcgtggcg atcacaacga cctgcagtac cgcctgaacg gcatcattct tcccgagggc
      481 atcagcgtct tcagccagac gctgagtcca aggctcatct cgtcgctgag tctcattacc
      541 ggcgcgctgc cggcggagta tggactgcgc accgcgggca tcgtcgatct caccaccaac
      601 agcggactgc tgcagccggg cggcagcgtg tcgctctacg gcggaagctt cgggactttc
      661 gagccggcgg tagagtacgg cggcaacacc ggccacctga gctactacgt cagcttcgac
      721 tacaagcagg ataaggtcgg catcgagtcc ccggacggca gcgccaatcc gctgcacgac
      781 cataccaagc agctgcacgg cttcgggtat ttcgagaaca tcgtcgatga gaacaaccgc
      841 ctgtcgctga tcctcgggac ctcggacgat gccttccaga ttccgaacca gcgcggcctg
      901 cagccgggtc tcgggctcac ggtcaacggc cagacgacgt tcctcagcga cgatctcaac
      961 gagacccagc acgaggccgc acagtacgcg atcgtcagct ggcagcacgc ggcaggcgcg
     1021 ctcaactggc agacctcgct ctcggcccgc tacaccacgc tgcacttcgc gcccgacttg
     1081 cttggcgacc tgctctacaa cggcatcgcc caggacgcat acaagtccga caccgccttc
     1141 ggctggcaga ccgacggctc ctacaagtgg aacgacgccc acacggtccg ggcgggcttt
     1201 tatatccagc acgatagcgc caagagcaac accacctcac gggtgctgcc gctcgatccg
     1261 gttacgggcg tgccgaccac cgacgtgccg gaggccatcg tcgacaacgg cagccagagc
     1321 cagtcgctcg agagcgtcta cctgcaggac gagtggcagg tgctcaagcc cctgaccatc
     1381 aactatggtc tgcgcttcga ccactacagc gcatattcca acggcagcca gctgagcccg
     1441 cgcgtgaatt tcgtctggga gctggccgag ggcaccaccg tgcacggcgg ctactcgcgc
     1501 tacttcacgc cgccgccctt cgagctcatc ggctcgccga cgctcgccaa gttcctcaac
     1561 accaccgcgt tgccaccggg ctcggtgaca ctggacgact cgccgatcgc cgcgcgcgcc
     1621 aactactacg act
//
LOCUS       DATLKL010000165         4537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_80901, whole genome shotgun sequence.
ACCESSION   DATLKL010000165 DATLKL010000000
VERSION     DATLKL010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80901"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VM713_02175"
     CDS             <1..869
                     /locus_tag="VM713_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH83099.1"
                     /translation="FTIRVPARIESAQTPPALHRVRPERPVARPIRGSLILVIDDDQT
                     VCELLARFLEREGFQVRAAAGGREGLALARELHPAAITLDIHMPDIDGWTVLAALKGD
                     PKLADIPVVLVTIDDDRSRGYSLGATEYMTKPVDRERLLSLLQDISTSVGRRVLLVDD
                     DEIMRESVRRVLEQEQWQVTGAANGRIALEQLAKSRPDVILLDLMMPEMDGFEFLVRL
                     REQSEMHDIPVLVLTAKDLSVEDHQRLNGYVDQVMQKSATELGQLLRELARMLPHTIE
                     RGHQGKAGEARA"
     gene            866..1240
                     /locus_tag="VM713_02180"
     CDS             866..1240
                     /locus_tag="VM713_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH83100.1"
                     /translation="MKILYVEDNEDNIYVLENRLRRQGHAVLIARDGASGVQLAMSER
                     PDVILMDLSLPILDGWEATRRLKAAPQTRDIPVIALTANAMAGDREKALAAGCDDFDT
                     KPVAMTRLLAKIDTLLPGGRAT"
     gene            1346..1723
                     /locus_tag="VM713_02185"
     CDS             1346..1723
                     /locus_tag="VM713_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH83101.1"
                     /translation="MNKILLIEDNELNRDMLTRRMQKRGYQIVTAADGEAGVAMAQNE
                     APALILMDVSLPGIDGWEATRRIRAAPATRHIPIIALTAYATPGDRETSLAAGCDDFD
                     TKPIDLPRLLGKIENLLKRGSPA"
     gene            1720..3312
                     /locus_tag="VM713_02190"
     CDS             1720..3312
                     /locus_tag="VM713_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH83102.1"
                     /translation="MIDRASLSHLRHELHTPLNHIIGYSELLLESAGAGGIVALEPKL
                     RELHESARQLMLLVDETLGRTRKEPGDIDLQRLDAQTSAPIASIIAIAQQLRLQTEQM
                     GAAEASQDLDHIASAARTLRTMIADGVTAPPVAPLVDSDTARLERLTAQASETGDILV
                     VDDNAENREMLSRRLARAGHRVQAVASGPEALALLGRQPIDVVLLDVLMPEMSGYDVL
                     QRLHAEKLLREIPVLMISALDEMDSVVRCIELGAEDYLPKPFDPVLLRARIGACLEKK
                     RLRDREAMHLRELGEWNQKLEQRVAEQVALLDRLSRLKRFFSPQLAELIVAGGADDPL
                     VTHRRELTVCFLDLRGFTAFAESVEPEEVMGVLRQYHAEMGRLILEHEGTLERFTGDG
                     MMVFFNDPVPVSNAAERTVRMAIAMRKRVSELIVSWSSMGYELELGIGIAQGYATIGA
                     IGYEGRWDYGAIGTVTNLAARLCGEAKPGQILVSKRLLAATADLFDVESVGELPLKGL
                     HRPVTAYNVLRARGSVTVAGGS"
     gene            complement(3261..3722)
                     /locus_tag="VM713_02195"
     CDS             complement(3261..3722)
                     /locus_tag="VM713_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HVH83103.1"
                     /translation="MSGEFRPEIELFNDSLARCLRGDRLFQHFYDAFLASSEEVRAKF
                     RNTDFRKQIRVLKSSFYMLVEYLALGWPECEAYLERIAAAHSKRERDVAPHLYDLWLE
                     CLLQAVKECDEQYSPQVDAAWRYVMGAGINFLKARYDPPATVTDPLARRTL"
     gene            4064..4444
                     /locus_tag="VM713_02200"
     CDS             4064..4444
                     /locus_tag="VM713_02200"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HVH83104.1"
                     /translation="MITADQLLAHAVGDYILQSDWMAHEKTKSTFAALCHIGTYILPF
                     LLLTHSPLALAAIAGSHFVIDRWRLSRYLVWVKNWMGRNRPWKECAATGYPPERPAWM
                     AVWLLIGVDNIMHILCNGLALQYL"
BASE COUNT          817 a         1430 c         1537 g          753 t
ORIGIN      
        1 catttacgat ccgagtgccg gcccgcatcg agtcggccca gacgccgcca gcgctgcacc
       61 gggtgcgccc cgagcgtccc gtggcacggc cgatcagagg ctcgctgatc ctcgtcatcg
      121 acgatgacca gacggtatgc gagttgctgg cgcgtttcct tgaacgcgag gggtttcagg
      181 tgcgcgcggc ggccggcggc cgcgagggtc tggcgctcgc ccgtgagctg cacccggcgg
      241 ccatcaccct cgacattcac atgccggaca tcgacggctg gaccgtgctc gcggcgctca
      301 agggtgaccc caagctcgca gacattccgg tcgtgttggt gactatcgat gacgaccgca
      361 gccgcggcta ctcgctcggc gccacggagt acatgaccaa gcccgtcgac cgcgagcgac
      421 tgctcagcct gctgcaggac atcagcacct cggtgggacg ccgcgtgctg ctggtcgacg
      481 acgatgagat catgcgcgag agcgtgcggc gggtgctcga gcaggagcag tggcaggtca
      541 cgggtgcggc caacggccgc atcgcgctcg agcagctcgc caagtcgcgc cccgatgtga
      601 tcttgctcga cctgatgatg ccggagatgg atgggttcga gttcctggtc aggctgcgcg
      661 agcaaagcga aatgcacgac attccggtgc tggttctcac ggccaaggac ttgagcgtcg
      721 aggatcacca gcggctcaac ggttacgtcg accaggtgat gcagaagagc gccaccgagc
      781 tgggacagct gttgcgtgaa ctggcccgca tgctgccgca caccatagaa cggggccatc
      841 aggggaaagc cggggaggct cgcgcatgaa gatcctgtac gtcgaggaca acgaagacaa
      901 catctacgta ttggaaaacc ggctcaggcg tcaggggcac gcggtgctga tcgcgcgcga
      961 tggcgcgagt ggcgtgcagc tcgccatgag cgagcggccc gatgtgatcc tgatggactt
     1021 gagtttgccg atactcgacg gctgggaggc gacgcggcgc ctcaaggcag cgccacagac
     1081 acgggacatt cccgtcatcg ccctgacggc gaatgccatg gccggcgacc gcgagaaggc
     1141 tctcgcggcg ggctgcgacg atttcgatac caagccggtc gcgatgacgc gcctgctcgc
     1201 caagatcgac acgctgttac ccggaggccg cgcgacctga cggcgccttg ccggccggtc
     1261 gcggctggtc aatgatcaag cggactgatt cgcgcctcgg cctgcgtccg agcagggacg
     1321 ccggccgtac cgccaggagg ggcgcatgaa caagatactg ttgatcgagg acaacgagct
     1381 caaccgcgac atgttgacgc ggcggatgca gaagcgaggg taccagatcg tgaccgcggc
     1441 ggatggtgag gcgggggtgg cgatggcgca gaatgaggcc ccggcgctga tcctgatgga
     1501 tgtgagtctg cccggcatcg acggctggga ggcgacgcgg cgcatcaggg cggcgccggc
     1561 gacccgccac atcccgatca tcgcgctgac cgcctacgcc acgcccggcg accgcgagac
     1621 ctcgctcgcc gcgggttgcg acgatttcga caccaagccg atcgacctgc cgcggctgct
     1681 ggggaagatc gagaacctgc tcaagcgagg atctccggcg tgatcgatcg cgccagcctg
     1741 agtcatctgc ggcacgagct gcacaccccg ctcaaccaca tcatcggcta cagcgagctg
     1801 ttattggaaa gtgccggcgc gggaggcatc gtggcgctgg agccgaagct gcgcgagctg
     1861 cacgaaagtg cgcgccagct catgctgctc gtcgatgaga cgcttgggcg cacccgcaag
     1921 gaacccggtg acatcgatct gcaacgcctc gacgcccaga cctccgcgcc catagcctcg
     1981 atcatcgcga tcgcccagca actgcggctg cagaccgagc agatgggtgc tgccgaggca
     2041 agccaggatc tcgatcacat tgcgagtgcc gcgcgcacgc tgcggacaat gatcgccgac
     2101 ggagtcaccg cgccgcctgt tgcgccgctg gtcgactcgg acacggcgcg gctcgagagg
     2161 ctcacagcgc aggccagcga gaccggcgac attctggtgg tcgacgacaa cgccgagaat
     2221 cgcgagatgc tgagccgccg actggcgcgc gccgggcatc gcgtgcaggc ggtggcgagc
     2281 gggccggaag cgctcgcgtt gctgggtcgg cagccgatcg acgtcgtgct gctcgacgtg
     2341 ttgatgccgg agatgagtgg ctacgacgta ttgcagcggc tccacgccga aaagttgctg
     2401 cgggaaatcc cggttctcat gatatcggcg ctcgatgaga tggacagcgt cgtgcgctgc
     2461 atcgagctgg gcgccgagga ctacctgccg aagcccttcg acccggtgct gctacgcgcg
     2521 cggatcggcg cctgcctcga gaagaagcga ctgcgcgatc gcgaagccat gcacctgcgc
     2581 gagctgggcg aatggaatca aaagctcgag caacgcgttg ctgagcaggt ggcgctgctc
     2641 gaccgactca gtcggctcaa gcggttcttc tcgccgcagc ttgccgagct catcgtggcg
     2701 ggcggggccg acgatcccct cgtgacccac cggcgcgagc tcacggtctg cttcctcgat
     2761 ctgcgcggtt tcactgcctt tgccgagagt gtcgagcccg aggaagtcat gggcgtgctg
     2821 cgccagtatc acgcggagat gggccggctg atcctcgagc acgagggtac gctggagcgg
     2881 tttacgggcg atggcatgat ggttttcttc aacgatccgg tgccggtatc caacgcagcg
     2941 gaacgcacgg tccgcatggc catcgcaatg cgcaagcgtg tcagcgagct gatcgtctcg
     3001 tggagcagca tgggttacga gctcgagctc gggatcggga tcgcacaggg ttacgccacc
     3061 atcggcgcaa tcggctacga aggacgctgg gactacggtg cgatcggcac ggtgaccaat
     3121 ctcgcggcgc gcctgtgtgg tgaggcgaag ccggggcaga ttctggtctc caagcggctg
     3181 ctcgccgcga ccgccgatct cttcgacgtc gaatcggtgg gcgagctccc tctcaaggga
     3241 ctgcaccgtc ccgtcacggc ctacaacgtt ctgcgtgcca gggggtcagt gacggtagcg
     3301 ggggggtcgt agcgcgcctt caggaagttg atgccggcgc ccatcacgta gcgccacgca
     3361 gcgtcgacct gtggcgagta ctgctcgtcg cattccttca cggcctgcag caggcattcg
     3421 agccacaagt cgtaaagatg cggtgcaaca tcgcgctccc gcttgctgtg agccgcggcg
     3481 atgcgctcga gatacgcctc gcactcaggc cagccgaggg ccagatattc caccagcatg
     3541 tagaaagagg acttcaagac cctgatctgc ttgcggaagt cggtgttgcg aaacttcgcg
     3601 cgcacctcct ccgaggacgc caggaatgcg tcgtagaagt gttggaagag ccggtcgccc
     3661 cgcagacagc gggcgagact gtcgttgaaa agctcgattt cgggcctgaa ctcaccgctc
     3721 attgcaacga cgctactcca gctgcttccg gaggatcaat cagggatttc cgcaggcagt
     3781 gcgctcgacc gtcggcccgg ggcgatgttg catcgcacgc ctcgcgaaga tgttgctgtg
     3841 aagcacaatc ctgagcacta caatatcggc gctcgaggtt gatacgcacg gccgcagagc
     3901 cttgtggcag aaatcgagga gtgaggaaca tcccgaccgg cgcctggcgg gatgaccggg
     3961 gggcatggcg ctgcatgatc ggtggcggca tctcagttcc gaggtcgcgg tatgactgcg
     4021 tggctgcggc tgcgccagca acgcgcgccg cccgtgctaa cgcgtgatca cggcagatca
     4081 actgttggct catgcggtcg gcgactacat cctgcagtcc gactggatgg cgcacgagaa
     4141 gaccaaaagc actttcgctg ccctatgcca tatcggcacc tacatactgc cgttcctcct
     4201 tctcacacac tcgccgctgg cgctcgcggc catcgcgggc agccattttg tgatcgaccg
     4261 ctggcggctg tcccgctacc tcgtgtgggt caagaactgg atgggcagaa atcgcccgtg
     4321 gaaggagtgc gcggcgacgg gctacccgcc cgagcggccg gcgtggatgg ccgtctggct
     4381 gctgatcggt gtcgataaca tcatgcacat tctctgcaat ggactggcgt tgcagtacct
     4441 gtgacggcat cggaccccgc cacgcgtccg ggcggccgca cgcgctgcgg ctgctgccac
     4501 agctgctgct gttcgcgctg ccgctggtca cgccgtt
//
LOCUS       DATLKL010000166         1955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_193599, whole genome shotgun sequence.
ACCESSION   DATLKL010000166 DATLKL010000000
VERSION     DATLKL010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193599"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..571)
                     /locus_tag="VM713_02205"
     CDS             complement(149..571)
                     /locus_tag="VM713_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVH83105.1"
                     /translation="MKPIPNGAQGRYVHRVEPAHLANRFKDSILPPVFSTPYLILIME
                     NAALNAIRKYLEEGESAVGTRVDVRHLVATPVGREVVGHAEVTGTDGRRISFRVWAMD
                     RGEAIGVGVHERTVVNAARIVEQMAAKYGTTEEKSSSR"
     gene            635..1153
                     /locus_tag="VM713_02210"
     CDS             635..1153
                     /locus_tag="VM713_02210"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVH83106.1"
                     /translation="MLDPQAAQSLAQYKSWADDLTLSAVAVLPPGEAARERPTLFKSI
                     IGTLNHSYVVDLIWQAHLEGREHGFTARNVVPHPELEALAAAQRRANDWLLAWAEAQS
                     AASLHEAVAFRLVSGEQGRMTRGEILLHIVNHATYHRGWVSDLFFQIPAKPPTTDWNI
                     FLLRGSRTSRAT"
     gene            complement(1167..1874)
                     /locus_tag="VM713_02215"
     CDS             complement(1167..1874)
                     /locus_tag="VM713_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVH83107.1"
                     /translation="MALLTVPYRGIIFDFNGVLFFDSDLQEQAWQVVAGKLRGREMTA
                     AEFALHMHGRSNAYVLSYLAGREMRGPELAEWIDVKESLFRNLCLTTPNRLLLSPGAQ
                     DLLESLARAGIPRTIATSSGITNVKFFTEHLHLDRWFDVDRLVYDDGARPGKPAPDMY
                     LAAAGNIGVEPSRCVVVEDAASGVAAAQAAGIGYIVGIGASAGHAELLTSRGAALIIE
                     TLRDFPRERLAGLTPRQ"
BASE COUNT          334 a          668 c          613 g          340 t
ORIGIN      
        1 cagcccgcaa ggcgatcctc gccatgccgc agactcaggg cgagtggcgc gaggtgcgga
       61 ttcgccagaa gaccggcgag acgcagaaac tcagggtgct gcagctgccc accaagcact
      121 agcacgcccg tgccgggggt cgagagcctc agcggctgct gcttttctct tccgtcgtcc
      181 cgtacttcgc cgccatctgc tcgacgatgc gcgcggcgtt gacgaccgtg cgctcatgga
      241 ccccgacgcc gatcgcctcc cctcggtcca tggcccacac gcggaatgag atccggcgac
      301 cgtccgtgcc cgtgacttcc gcatggccta ccacctcgcg cccgaccggc gtcgccacga
      361 gatggcgcac gtcgacgcgc gtaccgacgg cactctcgcc ctcttcgagg tatttcctga
      421 tggcattcag tgccgcgttc tccatgatga gaatcagata cggggtcgag aagaccggcg
      481 gcaggatgct gtccttgaat cgattcgcca gatgcgccgg ctcgacccga tgcacatagc
      541 ggccctgagc gccgttcgga atgggtttca tgcgaatcga gattgtaccc ttcgtgtata
      601 accaccggga cggacagact catcggtggc acccatgctg gatccgcaag cggcgcagtc
      661 gctcgctcaa tacaagagct gggccgacga cctcactctg agcgcggttg cggtgctccc
      721 tcccggtgag gccgcgcggg agcggccgac gctcttcaag agcatcatcg gcacgctgaa
      781 ccacagttac gtcgtcgatc tcatctggca ggcgcatctc gaggggcgcg agcacgggtt
      841 cacggcgcgc aatgtcgtcc cgcacccgga gctcgaggcg ctcgcggccg cgcagcggcg
      901 cgcgaacgac tggctcctcg cctgggccga ggcgcagtcg gccgcgagcc tgcacgaagc
      961 ggtcgctttt cgcctggtct cgggcgagca gggcaggatg acccgcggcg agattctcct
     1021 gcacatcgtc aatcatgcga cctatcaccg cggttgggtc agcgatctct tcttccagat
     1081 accagccaag ccgccgacga ccgactggaa catctttctg ctgcgcggct cccggacttc
     1141 ccgcgccacc tgattctccg cgactgtcat tgccgcggcg tcagcccggc aagtcgctca
     1201 cgcggaaaat cgcggagagt ctcgatgatc agcgccgcac ccctggaggt cagcagctcc
     1261 gcgtggcccg cggaggcacc gatgccgacg atgtagccga ttcctgcggc ctgtgccgct
     1321 gccacaccgg aggccgcatc ctcgaccacg acgcaacggc tcggctccac tccgatattg
     1381 cctgctgccg ccaggtacat gtcgggagcc ggcttgcccg ggcgggcacc gtcgtcgtaa
     1441 acgagcctgt cgacgtcgaa ccatctatcg aggtgcaggt gttcggtaaa gaacttcaca
     1501 ttcgtgattc cggacgaggt ggcgatcgtg cgcggaatgc cggcacgcgc gagcgattcg
     1561 aggaggtcct gcgcccccgg tgagagcagc agccggttgg gagtcgtgag gcacagattc
     1621 cggaacagcg actccttgac gtcgatccac tccgccagct ccggtcctct catttcgcga
     1681 cccgccagat agctcaggac gtacgcattc gatcggccgt gcatgtgcag cgcgaactct
     1741 gcggcggtca tttcccggcc ccgcaatttc cccgcgacca cctgccaggc ctgctcctgc
     1801 agatcggagt cgaagaacag cacgccgttg aagtcgaaga tgatcccgcg gtatggcacg
     1861 gtcagtaggg ccatgggcct cacctgtgcg atgcctgcgg gctggtcagc ctgcgaatga
     1921 gatcgacctc ggcctgccgg tagggtgtgc catcg
//
LOCUS       DATLKL010000167         1511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_204807, whole genome shotgun sequence.
ACCESSION   DATLKL010000167 DATLKL010000000
VERSION     DATLKL010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204807"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VM713_02220"
     CDS             <1..564
                     /locus_tag="VM713_02220"
                     /inference="COORDINATES: protein motif:HMM:NF044297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83108.1"
                     /translation="RLALDTRFPEEETLRLTVHAARPARLAVRVRLPYWTAGAQAQLN
                     GKPLAASGAASGYLAVERTWRDGDTLNVRLPMRLHTAPMPDDSSIQAVMYGPLVLAAR
                     LGTQGLTPENLRAPPTRPRKVPEYTTEPIPVAPLVLRSGGDPQSWLAPVPGRSLEFRT
                     RGQQDDLTFVPLNRVFDERYAVYFQVK"
     gene            561..>1511
                     /locus_tag="VM713_02225"
     CDS             561..>1511
                     /locus_tag="VM713_02225"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HVH83109.1"
                     /translation="MSTRFRRDPGYAGLTSPAATLPSPYYFDPAHYRLELSRIWYRHW
                     IYVCRSADIASARSFHTLEVGDQSLLMVRGEDGIARAFHNTCRHRGGALCRERAGRFP
                     AAGIVCPYHAWRYSLKGELVQTSSKVHGDGFELADFPLYGLPVTEWNGFLFTALTANP
                     PPFRASFDLPLNRLDDWRLADLVVGHRLEKTIRCNWKVFWENYNECLHCPGVHPRLAQ
                     LVPIFGRALLERRDDPTWQDHAADDDPKYGGGLRAGAESWSMDGKPAGAPFPGVSAAD
                     RKAGHVYMTSVPSVFIVARVDYVRVVRLLPRGPEETAMSIE"
BASE COUNT          230 a          558 c          484 g          239 t
ORIGIN      
        1 cgcctcgcgc tcgacacgcg ctttccggaa gaggagaccc tgcggctcac cgtgcacgcg
       61 gcgcgcccgg cgcgcctcgc ggtgcgcgtg cgcctgccct actggacggc cggtgcgcaa
      121 gcgcagctca acggcaagcc actcgccgca agcggagccg cgagcggtta tctcgcggtc
      181 gagcgcacct ggagggacgg cgacacactg aatgtgcgac tgcccatgcg gctgcacacc
      241 gcccccatgc ccgatgattc ctcgattcag gccgtgatgt acgggccgtt ggtgctggcc
      301 gcgcgcctcg gcacccaagg cctcacgccg gagaatctgc gtgcaccgcc cacgcgaccg
      361 cgcaaggttc ccgagtacac caccgagccg attcccgtcg caccgctggt gctgcgctcg
      421 ggaggggatc cgcaaagctg gctcgcgccc gtcccgggcc gcagcctcga atttcgcacg
      481 cgcggccagc aggatgatct cacgttcgtg cccctgaacc gcgtcttcga cgaacgctac
      541 gccgtatact tccaggtcaa atgagcaccc gattcaggcg cgaccccggc tacgcgggcc
      601 tcacgagccc agccgctacg ctgccctcgc cgtactactt cgacccggcg cactaccggc
      661 tcgagttgag ccgcatctgg taccgtcact ggatctacgt gtgccgctcg gctgatatcg
      721 ccagcgcccg cagcttccac acactggaag tcggcgatca gtcgctactc atggtgcggg
      781 gcgaggatgg catcgcgcgc gccttccaca acacctgccg gcatcgcggc ggggcgctgt
      841 gccgcgagcg ggccggccgt ttcccggccg cgggcatcgt ctgcccgtac cacgcctgga
      901 gatacagcct caagggcgag ctcgtacaga cttcctccaa ggtgcacggc gatggcttcg
      961 agctcgcgga tttcccgctc tacggcctgc ccgttaccga gtggaacggc ttcctcttca
     1021 cggcgctcac cgcgaacccg ccccccttcc gcgcgagctt cgatctgccg ctcaaccggc
     1081 tcgatgactg gcggctcgcc gacctcgtgg tcggccatcg gctcgagaag accatccgct
     1141 gcaactggaa ggtgttctgg gagaactaca acgaatgcct gcattgcccg ggggtgcacc
     1201 cgcgcctcgc gcagctcgtg ccgatcttcg gccgcgccct gctcgagcga cgcgacgatc
     1261 ccacctggca ggaccatgcc gcggacgatg atcccaagta cggcggcggc ctgcgcgcgg
     1321 gggccgaatc ctggtccatg gacggcaagc ccgccggcgc gcccttcccc ggggtgagcg
     1381 ctgcggaccg caaggccggc cacgtttaca tgacgtccgt gccgagtgtc ttcatcgtcg
     1441 cgcgcgtcga ctacgtgcgt gtggtgcgac tcctgccgcg gggcccggag gagacggcga
     1501 tgagtatcga g
//
LOCUS       DATLKL010000168         1901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_159853, whole genome shotgun sequence.
ACCESSION   DATLKL010000168 DATLKL010000000
VERSION     DATLKL010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159853"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VM713_02230"
     CDS             complement(<1..707)
                     /locus_tag="VM713_02230"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HVH83110.1"
                     /translation="MTAPLTAWVEGLGLLGPGLSGWPAAAQVLAGRVPYEPRPTVIAP
                     LECLPPAERRRTGKVVRLALTAGLEAVTSARADVTSLRAVFSSSGGDGENCHEICQVL
                     AGAERQISPTRFHNSVHNAASGYWGIATGATAASNALCAYDASFGAGLLEALAQVAID
                     ETPVVLVAYEADYPEPIRTARPIPDAFAVALLLAPAARQGSLARLTADLSGALAEALP
                     DPQLEALRTAVPAARSLP"
     gene            complement(704..>1901)
                     /locus_tag="VM713_02235"
     CDS             complement(704..>1901)
                     /locus_tag="VM713_02235"
                     /inference="COORDINATES: protein motif:HMM:NF006618.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HVH83111.1"
                     /translation="AVKPLALSAFTATSCLGRGLDATLAALEGERTGLAPCTFETVQL
                     ATCVGEVPGVDDERLPAELEEFECRNNRLALLALRQDGFDEAVRALVRRLGRKRIGVW
                     LGTSTSGILETELAYRRRDPASGALPADFRYRGAHNTFSVAAFVQRALGLTGPAAVIS
                     SACSSSAKVFASAQRAIEAGLVDAAVVGGVDSLCLTTLYGFHSLQLVARTPCRPFDTG
                     RDGLSISEAAAFALLEPPGDRVAAGAVLLLGTGESSDAWHMSAPHPEGRGAGAAMTQA
                     LTAARLAPGDIDYINFHGTGTPSNDAAEARAVAAVLGRRTPGSSTKGATGHALGAAGG
                     LEAVICALALRHALLPGGVNTRVLDPELGVSYLLGNRRAPLAHVMSNSFGFGGSNCSL
                     IFGRAA"
BASE COUNT          295 a          669 c          696 g          241 t
ORIGIN      
        1 ggcagactcc tggccgccgg caccgcggtg cgcagcgcct cgagctgcgg gtcgggcagc
       61 gcctccgcga gcgcgccgct caagtccgcc gtgagccgcg cgagcgatcc ctggcgcgcc
      121 gccggcgcaa gcagcagcgc cactgcgaac gcgtccggaa tcgggcgcgc ggtgcggatc
      181 ggctccggat agtccgcttc ataggcaacg aggaccaccg gcgtctcatc gatggcgact
      241 tgcgccaggg cctcgagaag gcccgcgccg aagctcgcat cgtaggcgca cagcgcgttc
      301 gaagctgcgg tggcgccggt ggcgatgccc cagtagccgg atgcggcgtt gtgcaccgag
      361 ttgtgaaagc gtgtcgggga gatctgccgt tcagcgccgg ccagcacctg acagatctcg
      421 tggcagtttt ccccgtcgcc ccccgaggag gagaacaccg cgcgcagtga ggtgacatcg
      481 gcgcgggcgc tggtgacagc ctcgagcccg gcggtcaggg ccagccgtac gaccttgccg
      541 gtgcggcgcc gctcggcggg cggcaggcac tcgagcggcg cgatcacggt cgggcgcggc
      601 tcgtacggga cgcggcctgc gaggacctgt gccgcagccg gccagcccga gagtcccgga
      661 cctaggaggc ccaggccctc gacccatgcc gtgagcggcg cggtcatgcg gcccgcccga
      721 agatgaggct gcagttcgag cccccgaagc cgaaggaatt gctcatgaca tgcgcgagcg
      781 gtgcgcggcg atttccgagc aggtagctca cgccgagctc gggatcgagg acccgcgtgt
      841 tgacgcctcc cggcaggaga gcgtgccgca gcgcaagcgc gcagatcacc gcttcgaggc
      901 ctccggcggc gccgagcgcg tgtccggtcg cgcccttggt ggagctgccg ggagtgcgcc
      961 tgccgagcac ggccgcgacc gcgcgggcct cggcggcgtc gttgctgggc gtgccggtac
     1021 cgtgaaaatt gatgtagtcg atgtcgccgg gggccaggcg cgccgcagtc agcgcctgcg
     1081 tcatggcggc acccgcaccg cgaccctcag gatgaggcgc cgacatgtgc caggcgtcgc
     1141 tcgactcgcc ggtaccgaga agcagcacgg cgcccgcagc gaccctgtcg ccagggggct
     1201 cgagcagggc gaacgccgcg gcctcactga tcgagagccc gtcacgcccc gtatcgaacg
     1261 gccggcaggg agtacgcgcc acgagttgca gcgaatgaaa gccatagagc gtcgtgagac
     1321 agagcgaatc gacgcctccc actaccgcgg catcgacgag ccccgcctcg atggcccgct
     1381 gcgcggaggc gaacactttg gcgctcgagg agcaggccga ggagatgacg gccgccggtc
     1441 ccgtaagtcc gagcgcgcgc tgcacgaagg ccgcgaccga gaaggtgttg tgtgcgccgc
     1501 ggtagcgaaa gtccgccggc aacgcgccgc tcgccggatc gcgtcgccgg taggcgagct
     1561 cggtctcgag gattcccgag gtgctggtac cgagccacac gccgatgcgc ttccgtccca
     1621 gccggcgcac gagtgcccgc acggcctcat cgaacccatc ctgccgcagc gcgagcagtg
     1681 ccaggcgatt gttgcggcac tcgaactcct cgagctccgc gggcagccgc tcgtcatcga
     1741 cgcccggcac ctcgcccacg caggtagcca gctggacagt ctcgaaagtg cacggggcga
     1801 gcccggtgcg ctccccttcg agcgcggcca gcgtcgcgtc gagcccgcgg ccgaggcagc
     1861 tcgtggcggt gaaggcgctc agcgcgagcg gcttcacggc a
//
LOCUS       DATLKL010000169         1670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_2043, whole genome shotgun sequence.
ACCESSION   DATLKL010000169 DATLKL010000000
VERSION     DATLKL010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VM713_02240"
     CDS             <1..568
                     /locus_tag="VM713_02240"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HVH83112.1"
                     /translation="KMTGAPRINRVQLNGADIEYDVRGSGEPVVFIHGAVLSDGFLPV
                     IEQAGIAENFRIVNYRRRGYAGSSRSVAGMTVQDWADDCIALLNHRPHLSLDMLGSEA
                     AEVLEPLTADPGAGPVAAAEARDQATALRQALARLPPEQRDAFLLQLEGDLSVEDVAA
                     ITHASFETVKSRLRYARTRLREMLAEYA"
     gene            565..1530
                     /locus_tag="VM713_02245"
     CDS             565..1530
                     /locus_tag="VM713_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HVH83113.1"
                     /translation="MSTLPPPFDPSDDDADEQYRRASERDPSRPSEATRRAVLAAAAR
                     LAAERARRDARRRWLSLIGAPVRMRPAIVGTIAAALLAGIIIAPQFLPESQRSPVTTP
                     PQAASAPATAPAASPIAEAPQAAPAAQLAAEERAPRPAPPAAASGAARTVPRLQARQA
                     PELNEMALKQSVTAKVTPEANLQGGAGAQDRGAAAGEASQAKAARSIAPLTAASAPVR
                     AQVPAAPPVSGGPEALRQAASAGDVTALRAQLASGTDIDARDAQGRTALMLATLNGRS
                     NAVAVLLTEGADPSAADGRGTTPLQAAVAADEREIIAMLRRYGAR"
BASE COUNT          269 a          578 c          605 g          218 t
ORIGIN      
        1 aaaaatgacc ggagcgccac gaatcaaccg agtgcagctg aacggagcgg acatcgagta
       61 tgacgtccgc gggtcggggg aaccggtggt atttatccac ggagcggttc tgtcggatgg
      121 gttcttgccg gtgatcgagc aggccggaat cgcggagaat tttcggatcg tcaactaccg
      181 gcgccgcggc tacgccggta gctcacgctc tgtggcgggc atgacggtgc aagattgggc
      241 ggatgactgc atcgcgttgt tgaatcaccg acctcatctc agcctggata tgctggggtc
      301 tgaggccgcc gaggtgctcg agccgctgac cgcagatcca ggcgcgggtc cggtcgcggc
      361 ggcggaggcg cgggatcagg cgacggcgct gcggcaggcg ctcgcgcgcc tgccgcccga
      421 gcagcgcgac gcctttttgc tgcagctcga aggagacttg agcgtcgagg acgtggcggc
      481 catcacccac gccagtttcg agaccgtcaa gagccgctta cgctatgcca ggacgaggct
      541 acgggagatg ctagccgagt acgcatgagc accttgccac cccccttcga tccgtccgac
      601 gacgacgccg acgagcagta tcggcgcgcc tcggagcgcg acccgagccg cccgagcgaa
      661 gccacccgcc gtgcggtgct tgcagctgcg gcccgcctcg ccgccgagcg cgcccgccgt
      721 gacgcgcgcc gccgttggct gtcgctgatc ggcgccccgg tgagaatgcg gccggcgatc
      781 gtcgggacga tcgcggcggc gctgctcgcg ggaatcatca tcgccccgca gttcctcccc
      841 gagagtcagc gttcacccgt cacgacgcct ccgcaggcgg ccagcgcgcc ggctaccgcg
      901 cccgccgctt ccccgattgc ggaggcgccg caggcggcac ccgccgcgca gctcgcggcg
      961 gaggagcgcg cgccgcggcc tgcgcctccc gcggccgcaa gcggcgctgc gcggacggtg
     1021 ccgcgcctgc aggcacgcca ggctccggaa ctcaacgaga tggcgctgaa gcagtccgtc
     1081 accgcgaagg tgacgcctga ggccaattta cagggtggcg ccggggcgca ggaccgtggc
     1141 gcagccgcgg gcgaggcatc gcaggcgaaa gccgcgcgct ccattgctcc gctcacggct
     1201 gcctcggccc cggtgcgggc gcaggtaccg gcagcgccac ccgtctcggg cggtccggag
     1261 gcgctgcgac aggctgcctc agccggtgac gtgacggcac tcagggcgca gctcgcgagc
     1321 ggcaccgaca tcgatgcgcg cgacgcgcag ggccgcacgg cgctcatgct ggcaacgctc
     1381 aacggacggt cgaatgccgt cgccgtgctc ctcacggagg gggccgaccc gagcgccgcc
     1441 gacggacgcg gcaccacgcc gctgcaggcg gccgtcgcag ccgacgagcg ggaaatcatc
     1501 gcaatgctgc gccgctacgg cgcccggtag agaaactccc tgaggctggg gaggtactcg
     1561 ggatagaagc tcacgtcgtt gtgggtagcg cccgacacga ggagcgtgtg ccggagcgat
     1621 ggcgcaattg ccgcgaccag agcagcggag cgagcgcgaa acacgacctc
//
LOCUS       DATLKL010000170         1721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_13123, whole genome shotgun sequence.
ACCESSION   DATLKL010000170 DATLKL010000000
VERSION     DATLKL010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13123"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..759)
                     /locus_tag="VM713_02250"
     CDS             complement(76..759)
                     /locus_tag="VM713_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH83114.1"
                     /translation="MRTILKVSAACLGCGVLAGAFANGGGAMPMPSVPARVASPEQQA
                     RDLYSDGVGYVKKADKADLEAAHASDGGKKERALRDARDRYASAHGKFEQAVRLDPQR
                     YEAWNYVGYTSRKLGHYDDALAAYDRALALKPDYPDAIEYRGEAYLAVNRLADAKQAY
                     LDLFAGNRALAAKLLTAMKDWVAAQRAAPASDHAASLDELDQWIQERTQIAAQTAALT
                     RAGTAAAWR"
     gene            complement(900..1664)
                     /locus_tag="VM713_02255"
     CDS             complement(900..1664)
                     /locus_tag="VM713_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HVH83115.1"
                     /translation="MTQLFGLYLIACPETGEALAVDPLEWRLCLDAARRRGWQITQIV
                     NTHEHSDHTGGNAALKDATGAKVLAHAGAAARIGGVDRGLQRGDLIRVGRTVELECLD
                     TPGHTMTHVCLLSHSAAPALYCGDTLFNAGAGNCYNGGDPQALYDTFVTQLARLDEAT
                     RVFPGHEYLARNLEFTLDREPANRDAAALLESARGADPAAATVTTLAEEKRINTFFRL
                     QNRQVIARLREKFPDLGEHPDARTVFVRLRELRNRW"
BASE COUNT          229 a          583 c          637 g          272 t
ORIGIN      
        1 ccccggtgcc cggcgcgctc ccggccgtca cgccgcacgg cgccgcactc gcgagcagcg
       61 cggcgcaggc cgccctcagc gccaggcggc cgcggtgccg gcgcgcgtga gggccgcggt
      121 ctgggcggcg atctgcgtgc gctcctggat ccactgatcg agctcgtcga ggctcgccgc
      181 atggtcgctg gccggggcgg cgcgctgcgc ggcgacccag tccttcatcg cggtgaggag
      241 cttggccgcg agggcacggt tgccggcgaa gaggtcgagg taggcctgct tggcatcggc
      301 gagtcgattc accgcgagat aagcctcgcc gcgatactcg atcgcgtccg ggtagtcggg
      361 cttgagcgcg agcgcccggt cgtaggcggc cagtgcgtcg tcgtagtggc cgagcttgcg
      421 gctcgtgtag ccgacgtagt tccaggcctc gtagcgctgc gggtcgagcc gcacagcctg
      481 ctcgaacttg ccgtgcgcgg aggcatagcg gtcgcgggca tcgcgcaggg cgcgctcctt
      541 cttgccgcca tcgctcgcgt gcgcggcctc aaggtccgcc ttgtcggctt tcttcacgta
      601 gccgaccccg tcgctgtaga ggtcgcgggc ctgctgctcg gggctcgcga cgcgtgccgg
      661 cacggacggc atcggcatcg ctccgccgcc gttcgcgaag gcgcccgcga gcacgccgca
      721 gccgagacac gccgccgaca ccttgagaat ggtacgcatg agggtcctcc tgacttgagc
      781 ctatgctgcc gccgcggcgg cacacgccgc aagggccgca ggcgttccgc gcaccgcgcg
      841 cttgttcccc cacacgcgcg ctccgcacgc gtccccggtg acccgccgcc gcccgtccct
      901 caccagcggt tgcgcagctc gcgcaggcgc acgaacacgg tgcgggcatc cgggtgctcg
      961 ccgaggtccg ggaacttctc ccgcagccgc gcgatcactt ggcggttctg cagccggaag
     1021 aaggtgttga tgcgcttctc ctcagccagg gtggtgaccg tggcggcggc gggatccgca
     1081 ccgcgcgcgc tctcgaggag cgcggcggcg tcgcggttcg caggctcccg gtcgagcgtg
     1141 aactcgaggt tgcgcgcgag gtactcgtgg cccgggaaga cgcgcgtcgc ctcatcgagg
     1201 cgcgcgagct gtgtgacgaa ggtgtcgtag agcgcttgcg ggtcgccgcc gttgtagcag
     1261 ttaccggcgc cggcgttgaa cagcgtgtcg ccgcagtaga gcgcgggcgc agcgctgtgg
     1321 gacaggaggc acacgtgggt catggtgtgc ccgggagtat cgaggcactc gagctcgacg
     1381 gtacgcccga cgcggatgag gtcgccgcgc tgcaagccgc gatcgacgcc gccgatgcgc
     1441 gcggccgcgc cggcgtgcgc caggaccttg gcgccggtgg cgtccttgag ggcggcgttg
     1501 ccgccggtgt ggtcactgtg ctcgtgcgtg ttcacgatct gggtgatctg ccagccgcgg
     1561 cgccgcgccg catcaaggca caggcgccat tcgagcggat cgaccgcgag cgcctcgccg
     1621 gtctcgggac aggcgatgag atataagcca aaaagttgtg ttatttagct cttaaaagta
     1681 ccttagcggt gctcatcatg ggaggacgga gatcatgaaa g
//
LOCUS       DATLKL010000171         1816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_80961, whole genome shotgun sequence.
ACCESSION   DATLKL010000171 DATLKL010000000
VERSION     DATLKL010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80961"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..>1816)
                     /gene="mutS"
                     /locus_tag="VM713_02260"
     CDS             complement(168..>1816)
                     /gene="mutS"
                     /locus_tag="VM713_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HVH83116.1"
                     /translation="PLTGQPLLRGRYQALAALIEARRFVPLRELLVGVGDIERILARV
                     ALRSARPRDLTQLRSSLRLLPALRAALAPIDSPLLRTLHGRIGEHAAELTLLAAAIAA
                     EPGTMVRDGDVIAAGYDRELDELRRIATHTDEFLLDLERRERERSGLAALKLGYNRVQ
                     GFFIEVSRRDAERVPKDYIRRQTVRSAERFITAELKSFEDRVLGARDRALARERELYE
                     QVLTQLGGALAPLQGTAGALAELDTLAALAERAAALEWTAPTLVEEPRLVIEAGRHPV
                     VARFADAPFVPNDLRLDGARRMLIITGPNMGGKSTYMRQAALIVLLAHIGSYVPADAL
                     VVGPIDRIFTRIGAADDLAGGRSTFMVEMTEAANILHNATARSLVLLDEIGRGTSTFD
                     GLSLAWAMARDIATRVRAFTLFATHYFELTTLALEVEGCANVHLDATEHGDGIVFLHS
                     VKDGPANRSYGLQVAQLAGVPLPVIGAARDYLEALESERDRQRATGNGAAAASQTELP
                     LFTPTSDADERLRAALAAIDPDDLTPRAALEELYRLRRLL"
BASE COUNT          270 a          600 c          683 g          263 t
ORIGIN      
        1 acttcgacga ggagaacgac ggcacagtcg cagtcagcga gacacacctg ccgggagcca
       61 ccgatcacat cgtgctgccg gtcagtcacc tggggatgct ggtatcggcg cgcgtggcgc
      121 acgagaccgg cgtgttcctc acccaggggc ggttttcgct gcgttagcta cagcaggcgc
      181 cgcagccggt agagctcctc gagcgccgcg cgcggcgtaa ggtcatccgg gtcgatggcc
      241 gcgagtgcgg cgcgcagccg ctcgtctgcg tcgctggtgg gtgtgaagag cggcaactcg
      301 gtctgggacg cggccgccgc accgttgccg gtcgcgcgct gccggtcacg ttcggactcg
      361 agcgcctcga ggtaatcccg ggcggcgccg atcacgggca gcggcacgcc ggcgagctgc
      421 gccacctgca agccgtagct gcgattggcg ggtccgtcct tcacggagtg aaggaacacg
      481 atgccatcgc cgtgctcggt tgcatccagg tgcacattgg cgcagccttc cacctcgagc
      541 gcgagtgtcg tgagctcgaa gtaatgggtg gcgaacagcg tgaaggcgcg tacgcgcgtg
      601 gcgatgtcgc gcgccatcgc ccaggcgagg gacaggccgt cgaaggtgct ggtgccgcgc
      661 ccgatctcat cgaggagcac gaggctgcgc gcggtggcgt tgtgcaggat gttggccgct
      721 tcggtcatct ccaccatgaa ggtcgagcgc ccgccggcga gatcgtcggc ggcgccgatg
      781 cgggtgaaga tacggtcgat cggtcccacg acgagagcgt cggcgggcac gtagctgccg
      841 atgtgcgcga gcagcacgat gagcgccgcc tgacgcatgt aggtcgactt cccacccatg
      901 ttggggccgg tgatgatgag catgcggcgc gccccatcca gcctgagatc gttggggacg
      961 aagggcgcat cggcgaagcg cgcgaccacc gggtggcgtc cagcctcgat cacgaggcgc
     1021 ggctcctcga ccagggtggg cgcagtccac tcgagcgcgg cggcccgctc ggccagcgcc
     1081 gccagcgtat cgagctcggc cagggcgccg gcggtcccct gcaacggtgc cagcgcgccg
     1141 ccgagctgcg tcagcacctg ctcgtacagc tcgcgctcgc gcgcgagcgc tcggtcgcgc
     1201 gcgccgagca cgcggtcctc gaagctcttg agctccgcgg tgatgaagcg ctcggcagaa
     1261 cgcacggtct ggcggcggat gtagtctttc ggtacgcgct cggcgtcgcg ccgcgagact
     1321 tcgatgaaga atccctgcac ccggttgtag ccgagcttga gggccgcgag cccgctgcgt
     1381 tcgcgctcgc gccgctcgag gtccaggagg aactcgtcgg tgtgggtcgc gatgcggcgc
     1441 agctcgtcga gctcgcggtc gtagcccgcg gcgataacgt cgccgtcgcg caccatggtg
     1501 ccgggctcgg cggcgatggc ggctgcgagg agcgtcagct cggcggcgtg ctcgccgatt
     1561 cgaccatgca gcgtgcgcag gagcggcgag tcgatcgggg cgagcgcggc gcgcaatgcg
     1621 ggcaggagcc taagcgagct gcgcagctgt gtgagatcgc gtggccgcgc cgagcgcagc
     1681 gcgacccggg cgagaatgcg ctcgatgtcc ccgaccccga cgaggagctc gcgcagcggc
     1741 acgaaacgtc gcgcctcgat cagagccgcg agcgcctgat agcggccgcg cagcagcggc
     1801 tgcccggtga gcggcc
//
LOCUS       DATLKL010000172         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_114828, whole genome shotgun sequence.
ACCESSION   DATLKL010000172 DATLKL010000000
VERSION     DATLKL010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114828"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VM713_02265"
     CDS             <1..744
                     /locus_tag="VM713_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HVH83117.1"
                     /translation="TEARRFLVLPHPAAASSRAPPAPAASPEAARRLFRAGRSVPGTP
                     AEAYLRARGITARLDWPSLRYHPSVYYREVENAPLEQWPVLLAAVTAHDGTITGILRT
                     WLDRHRPAKAPLADPRRAMGHLLGNAVRFGKASAILAAGEGVETMLALKSVLPRLPMA
                     AGLSANHLAALELSPPLHRLYVARDNDAAGRHAAEGLRERAAGIEVRELVPVHADFNL
                     DLCRLGADGLRTRLAQQLLPSDRARFLNR"
     gene            1182..2159
                     /locus_tag="VM713_02270"
     CDS             1182..2159
                     /locus_tag="VM713_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2493 domain-containing protein"
                     /protein_id="HVH83118.1"
                     /translation="MSRNQSTYLFSDADTDPEFHTASATAHLLEELSLYGHHPGPNEP
                     DPRPLPETEAVRGQLGAMVEAFSAMLTGSRLEDDLADLLWSFVNLFHRKVDRIERELD
                     VNEQAQRRGQTEQDGSEVKSVELERLIAQGLSLIERRNAFEFVRDHAAELFEVETGSA
                     WRPRTGSMVNHRALTASAIDSREFIAARRRADAELLAPAGARIAFTGGTDCNDHEAIW
                     AALDKVHAKHADIVLLHGGSPRGAERIAACWADNRKVAQVVFKPDWAREGRAAPFKRN
                     DRLLEALPIGVVVFPGSGISANLADKAKKLGIPVWRFDKTAAATINGAA"
BASE COUNT          349 a          856 c          702 g          362 t
ORIGIN      
        1 accgaggccc gccgctttct ggtcctgccg catcctgccg ccgccagcag ccgcgcgcct
       61 cccgcaccgg cggcatcgcc ggaagcggcc cgccgcctgt tccgcgccgg ccgttccgtc
      121 cccggcaccc cggccgaagc ctatctgcgt gcgcgcggca tcaccgcacg cctcgactgg
      181 ccgtccttgc gctaccaccc gtcggtctat taccgcgaag tcgagaatgc tccgctcgaa
      241 cagtggcccg tcttgctcgc cgccgtcacc gcgcatgacg gcacgatcac cggcatcctg
      301 cgcacctggc tcgatcggca ccgtccagcc aaggcgccgc ttgccgatcc gcggcgggcc
      361 atgggccatc tgctcggcaa tgccgtacgg ttcgggaagg cctcggcgat cctcgcggcc
      421 ggcgaaggcg tcgagaccat gcttgcgctc aaatccgtgt tgccgcgcct gcccatggcc
      481 gcgggccttt cggccaacca cctcgccgca ctggagctgt ccccgccgct gcatcgcctc
      541 tatgtcgccc gcgacaatga cgcggccggt cgccatgccg cggagggctt gcgcgaacgc
      601 gccgccggca tcgaggtccg cgagctggtc ccggtgcacg ccgacttcaa cctcgacctc
      661 tgccgcctcg gcgccgacgg gctgcgtaca cggctcgcgc agcagctact cccgtccgac
      721 cgcgcacgct tcctgaaccg ataactcgtc ccgatgcagg acggccatcg cggctatttc
      781 gcccggtgcc gcgccggtaa ggagaacggg cttcgttgcg gcggcgatgt gccctcgccg
      841 gaaagagccg cgcggcggcc ttcacgagcg gcgatctgcc ggaggcgccg ggcggcggct
      901 ggtcttttcg atcgatacaa ggagccgccg ccctgagccg gtttgcaatg gtgcggggac
      961 aactattttc cgccgccgcc ccatgttttt gtccgtcgcg cgaggcgcga tgattttctg
     1021 cggagggccg gctttgcatc gcgaagcaaa atagttgccc cgcgccatcc tccgctttgc
     1081 tccggccctt gcgggtgcaa accgtctccg cccccggcgg gcgatcgccg tgaaggccgc
     1141 gatgggcgcg gctgatcctc cggcaagaaa ggacacccgc catgtcacgc aaccaaagca
     1201 cctacctctt ctccgacgcc gacaccgatc cggaattcca cacggcttcc gcgaccgcgc
     1261 atcttctcga agagctttcc ctctacggcc atcaccccgg cccgaacgaa cccgatccgc
     1321 ggccgctgcc cgaaaccgaa gctgtccggg gccagctcgg cgccatggtc gaggccttca
     1381 gcgcgatgct gaccggcagc cgcctcgagg acgatctcgc cgatctcttg tggtcgttcg
     1441 tcaatctgtt ccaccgcaag gtcgaccgca tcgagcgcga gctcgacgtc aacgagcagg
     1501 cgcagcgccg cggccagacc gaacaggacg gctccgaggt caagtcggtc gagctcgagc
     1561 gcctcatcgc ccaggggctg agcctgatcg agcggcgcaa cgcattcgag ttcgtacgcg
     1621 accatgccgc cgaactcttc gaggtcgaga ccggctccgc ctggcggccg cgcaccggat
     1681 ccatggtcaa tcaccgggcg ctcaccgcct cggcgatcga cagccgggaa ttcatcgccg
     1741 cccgccgccg tgccgacgcc gagctgctcg cccctgccgg cgcccgcatc gccttcaccg
     1801 gcggcaccga ctgcaacgat cacgaagcga tctgggcggc actcgacaag gtgcacgcca
     1861 agcacgccga catcgtgctg ctgcatggcg gcagcccccg cggcgccgag cgcatcgccg
     1921 cctgctgggc cgacaatcgc aaggtcgcgc aggtcgtctt caagcccgac tgggcgcgcg
     1981 aaggccgtgc cgccccgttc aagcgcaacg accgcctgct cgaggcgttg ccgatcggcg
     2041 ttgtcgtatt tcccggctca ggaatctccg cgaacctcgc cgataaggcg aagaagctcg
     2101 ggatcccggt atggcgcttt gacaagacgg cggcggcaac gatcaacggc gccgcctgag
     2161 gcgccgtttt tccgctgtcg aaatccgcgc ggtcggcagg ccgcgcggtt ttcgaccata
     2221 actttcgaca ctggtaagcc attgaaatcg ctggccgaca atttttcac
//
LOCUS       DATLKL010000173         3775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_159893, whole genome shotgun sequence.
ACCESSION   DATLKL010000173 DATLKL010000000
VERSION     DATLKL010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159893"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VM713_02275"
     CDS             complement(<1..1034)
                     /locus_tag="VM713_02275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HVH83119.1"
                     /translation="MTGWRTRSVSPEEWLHAAQQLADEDAQLAALWASGGERGAPTVR
                     AAFFTGTGPLLLSLPLDSTAAPYPGLEEFFPCATRMQRAAADLSGVRASDPDTRPWLR
                     HNAWPEAYRPLRDPPWQPPPGAPAIDAYEFVRVEGEGVHEIPVGPVHAGIIEPGHFRF
                     SVVGEKVLRLEERLGYVHKGIERRFTELPILEAHRLAARVSGDSAVAFSWAYCQALET
                     LAGVTIPPRAAWLRALCLELERVANHLGDLGALGNDAGFAFGLAQFSRLKERQLRACA
                     AALGQRYLMDAIVPGGVAVDLTGSGAELLHGCAEEIAREARTLRTIYDEHPGVRDRFT
                     GAGTVTPALAA"
     gene            complement(1036..2493)
                     /locus_tag="VM713_02280"
     CDS             complement(1036..2493)
                     /locus_tag="VM713_02280"
                     /inference="COORDINATES: protein motif:HMM:NF005045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HVH83120.1"
                     /translation="MELLVLLATPLAGALTLGVLGARRFAPELNIGFSLLTFLAAVAL
                     AVRVVAAGRLVEAHDQFFIDAFNVFLVTLTAFVGLTTAIFSRPYMRVEMEHGRVRPAQ
                     LRLYHSMYQLFMSTMLLALTTNNMGLLWVAMEAATLSTVLLVTLYRTAASLEAGWKYF
                     ILCGVGIAQALFGTILLYFAAERAIGAEGVSALLWTHLNAVKGQLEPAVLSLAFVFLL
                     VGYGTKVGLVPLHNWLPDAHAEGPTPISAVLSGLLLNVAIYAVVRCKVLVEGSLHSAL
                     PAQMLMGFGLLSVVLAAFLLWRQRDIKRLFAYSSIEHMGIITFAFGMGGAVANFAALL
                     HMTVHSLTKSAIFFAAGHASQKAGSQLMDQIRGLITLSPTIGWGLMMGSLAILGMPPF
                     GVFASEFLILTTAMREQPWATPILLTALAVAFAAIFGRVQPMVFGETTAKRLPHPPAL
                     LPVFLHLGLVLVLGLYIPPYLADWYRAAARLIGGL"
     gene            complement(2495..3181)
                     /locus_tag="VM713_02285"
     CDS             complement(2495..3181)
                     /locus_tag="VM713_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83121.1"
                     /translation="MNGVPLILQLLNFCAALLLLLSFSMLSQRRIVNLVNLLAVQGGV
                     LLLATLLLAARTGQHHLYLSAALTLGLKVILLPWLLHRLIRRLGVYWDAEPLLNISGT
                     MLVGVLVVVFAFGLAQPISQLANTATRNAIGIAVAVVLLSFLMMITRRKAMSQVVGFL
                     SMENGLFFGAMSATYGMPMVVEFGVALDVLVAVLVLGIFFFQIREQFDSLDLHHLESL
                     REDATKGGRT"
     gene            complement(3186..>3775)
                     /locus_tag="VM713_02290"
     CDS             complement(3186..>3775)
                     /locus_tag="VM713_02290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HVH83122.1"
                     /translation="GTAFGTLGARREMMVGFLAEPALLMIIFVASLISATTALPAITE
                     HLATQSLGLYPSLAFTAVAFTMVLLAENARIPIDNPATHLELTMIHEAQLLEYSARHL
                     ALMEWAAALKLFNYACIGFALFIPWGVSTGRSGPLALLGSIPVLAAKLIVAGAVLALI
                     ETVSAKLRVFRAPEFLATAFLFAVLGLLVHLLLGA"
BASE COUNT          769 a         1227 c         1283 g          496 t
ORIGIN      
        1 gcggcgagcg ccggcgtaac cgtgccggcg ccggtgaaac gatcgcgaac tccggggtgc
       61 tcgtcgtaga tcgtgcgcag agtgcgcgcc tcgcgtgcaa tctcctcggc gcaaccgtgc
      121 agcagctcgg cgccggagcc cgtcaggtcg accgccacac cgcccgggac gatggcgtcc
      181 atcagatagc gctggccgag cgcagcggcg caggcgcgca gttgccgctc cttcaggcgt
      241 gagaactgcg cgagaccgaa ggcgaagccg gcgtcgttcc ccagcgcgcc aaggtctccc
      301 aggtggttgg cgacgcgctc gagctcgagg cacagcgcac gcagccatgc ggcgcgcggc
      361 gggatagtga cgcccgccag agtctccagt gcctggcagt aggcccagga gaacgccacg
      421 gccgagtctc ccgacacccg cgccgccaaa cggtgtgctt cgaggatcgg cagctcggtg
      481 aaacgccgct ctatgccctt gtgcacatac ccgagccgct cctcgagccg caacaccttt
      541 tcccccacga ccgagaagcg aaagtgtcca ggctcgatga tgcccgcgtg cacgggtccg
      601 acgggaatct catgcacccc ctcgccttcg acgcgcacga actcgtatgc gtcgatcgcg
      661 ggcgcgccag gcgggggttg ccacggtgga tcccgcagcg gccggtaagc ttccggccac
      721 gcgttgtgcc gcaaccaggg ccgggtgtcg ggatccgacg cacgcacgcc ggaaagatcc
      781 gccgccgccc gctgcatgcg tgtggcacat ggaaagaact cctcgagccc cggatagggt
      841 gccgccgtgc tgtcgagggg caggctgagg aggagcggac ccgtgccggt gaagaaggcc
      901 gcgcgcacgg tcggcgcgcc acgctcgccg ccgctcgccc agagcgccgc aagctgcgcg
      961 tcttcgtctg caagctgctg cgccgcatgg agccattcct ccggtgaaac gctgcgggtg
     1021 cgccagcctg tcatatcaaa ggcccccgat cagacgcgcg gccgcccgat accaatccgc
     1081 gaggtacggc gggatgtaga gcccgaggac gagcaccagg ccaagatgca ggaacacggg
     1141 cagcagtgcc ggcgggtgcg gcaggcgctt cgcggtcgtc tcgccgaaca ccatcggttg
     1201 cacccggccg aagatggcgg cgaacgccac cgcgagcgcc gtcagcagta tcggcgtcgc
     1261 ccagggctgc tcgcgcatcg ccgtggtgag gatcagaaac tcgctcgcga acacgccaaa
     1321 gggcggcatc ccgagaatgg cgagcgatcc catcatgagg ccccagccga tggtcggact
     1381 cagcgtgatg aggccacgga tctgatccat gagctgcgag ccggctttct gcgaggcgtg
     1441 gcccgctgca aagaaaatcg cagacttggt gagggagtgc accgtcatat gcagcagcgc
     1501 ggcgaagttc gccaccgcgc cgcccatgcc gaaagcgaag gtgatgatgc ccatgtgctc
     1561 gatcgaggaa taagcgaaca ggcgcttgat gtcgcgctgg cgccagagca ggaacgcagc
     1621 gagcaccacc gacagcagcc cgaagcccat cagcatctgc gcgggaagcg ccgaatgcag
     1681 cgagccctcg accagcacct tgcagcgcac gacggcgtag atggccacat tcagcagtag
     1741 ccccgagagc acggcagaga tcggggtcgg gccctccgcg tgcgcgtccg gcagccagtt
     1801 gtgcagcggc acgagcccga ccttagtccc gtagccgacc agcagaaata cgaacgcgag
     1861 actcagcact gcgggctcga gctgcccttt tacggcattc agatgcgtcc acagcaaggc
     1921 gctcacgccc tcggcgccga tggcgcgctc ggccgcgaag tacaagagga tggtgccgaa
     1981 gagcgcctga gcgatgccca cgccgcacag gatgaaatac ttccagccag cctcgagact
     2041 cgccgcggtg cgatagagcg tcaccagcag caccgtcgag agcgtcgcgg cttccatggc
     2101 cacccacagc aatcccatgt tgttggtagt gagcgcgagc agcatcgtcg acatgaagag
     2161 ctgatacatg ctgtggtaaa ggcgcagttg cgccgggcgc acccggccgt gctccatctc
     2221 cacgcgcatg tagggccgcg agaaaatcgc ggtcgtgagc ccgacgaagg ccgtgagcgt
     2281 caccaggaac acgttgaagg cgtcgatgaa aaactgatcg tgggcctcga ccagacggcc
     2341 cgcggcgacg acgcgcactg ccagcgcgac cgcagccaga aaggtgagca ggctgaagcc
     2401 gatattgagc tccggggcga agcggcgcgc cccgagaacc ccgagcgtca gcgcgccggc
     2461 gagaggcgtg gcgagaagca cgagcagctc cacgctaggt cctcccccct ttggtggcgt
     2521 cctcgcgaag cgactcgagg tgatgcaggt cgaggctgtc gaactgctca cggatctgga
     2581 agaagaagat gccgagcacc agtactgcga ccagcacatc gagcgcgacc ccgaactcca
     2641 cgaccatcgg catcccgtag gtcgcgctca tggcgccgaa gaacaggccg ttctccatcg
     2701 acaggaagcc cacgacctgg gacatggcct tgcggcgcgt gatcatcatc aggaaggaga
     2761 gcagcacgac ggcgacggcg atgccgatcg cattacgggt cgcggtgttg gcgagctgcg
     2821 agatcggctg ggcaaggccg aacgcgaata ctacgaccag cacgccgacg agcatcgtcc
     2881 ccgagatatt gagcagcggt tcggcatccc agtagacccc gaggcgtcgg atcagccgat
     2941 gcagcagcca aggcagcagg atcaccttga ggccgagcgt gagggccgcc gagaggtaca
     3001 ggtgatgctg cccggtgcgt gcggccaaca gcagcgtcgc gagcagcagc acgccgcctt
     3061 gtacggcgag caggttaacc agattcacga tgcgtcgctg cgacagcatg gaaaacgaca
     3121 agagcagcag cagcgcggca cagaagttca acagctgcag aatcaggggg acaccgttca
     3181 cggcttcagg ctcccagcag caggtgcacc aggaggccga gcaccgcaaa gagaaatgcc
     3241 gtggcgagaa actccggggc gcggaatacg cgcagcttgg cggacacggt ctcgatcagg
     3301 gcgaggacgg cgcccgcgac gatgagcttg gcggccagca ccggaatgga cccgagcagg
     3361 gccagcggac cgctccggcc cgtcgataca ccccagggaa tgaagagcgc aaagccgatg
     3421 caggcgtagt tgaagagctt cagagcggcg gcccactcca tgagcgccag atgacgcgcc
     3481 gagtactcga gcagctgcgc ctcatgaatc atggtcagct cgagatgcgt ggccgggttg
     3541 tcgatcggaa tgcgtgcatt ctcggcaagc agcaccatgg tgaaagccac cgccgtaaag
     3601 gcgaggctcg gatagaggcc gagcgactgt gtcgcgaggt gctcggtgat cgcgggcagc
     3661 gcggtggtcg ccgagatcag cgaggcgacg aagatgatca tgagcaacgc cggctcggcg
     3721 aggaacccca ccatcatttc ccggcgcgcg ccgagtgtgc cgaaggcggt gccga
//
LOCUS       DATLKL010000174         2249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_47115, whole genome shotgun sequence.
ACCESSION   DATLKL010000174 DATLKL010000000
VERSION     DATLKL010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47115"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2230)
                     /locus_tag="VM713_02295"
     CDS             complement(<1..2230)
                     /locus_tag="VM713_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HVH83123.1"
                     /translation="MRAELAQARRAGRRAVAIVFVHGWRHPQHERRAAATARELGFEE
                     VSVSHELSPLVRYVTRADTTVLNAYLALPLRAYLGGLKAQLHALDARSRLTLMQSNGG
                     LADAAAFHAMSSVLSGPAGGLIGMRWVGERAGFAKLIGFDMGGTSTDVSLIDGELPQR
                     FEHTIAGVRLMQPMLDVHTIAAGGGSILSFRDGRFAVGPASAGAEPGPACYRRGGPLT
                     LTDVQVLLGHLRPDTLPAVFGQDGRSRIDPDVVAAAFAELAARASAAGAQHCPAEVVA
                     EAFLEVGVESMANAIRQVSARQGLDAGDFTLFCFGGAAGQHACRVARAAGMRRILVHP
                     LASVLSAFGIGVADRLAVRRASLRQGLSSQGLAAARAALALLESQAREELAAFGAEPG
                     RARVALFLEVRAGDSDVTLSVPLGSLAEVHERFHAEHLKRFGFAAHTLDVVIEALRAE
                     ARLASLDGARLAMPAPRTRATPPRTVRAWFDGWREVPLVPMGELEGTLAGPALIVEPH
                     STVVVENGWRASALAGGELLLESEPAPPAAPAAAATDPARIEISNNLFMHIAEQMGEV
                     LKATAQSVNIRERLDYSCALFDSRGGLVANAPHMPVHLGSMGASVRAVIETRAAQLRP
                     GDSWLLNSPYHGGTHLPDMTVVTPVFVADAERVDFFVASRAHHADIGGMTPGSMPPFS
                     RNIEEEGALFECFHLVSDSTLREPELRAALAAGRWPARAPEQNLADLRAQVAANARGI
                     AEV"
BASE COUNT          327 a          816 c          822 g          284 t
ORIGIN      
        1 gaacctccgc gatgccgcgc gcgttggcgg cgacctgggc gcgcaggtcg gcgaggttct
       61 gctcaggtgc gcgcgccggc cagcggccgg ccgcgagcgc ggcgcgcagc tcaggctccc
      121 tcagggtcga gtcgctcacc aggtgaaagc actcgaacag cgctccttcc tcttctatgt
      181 tgcggctgaa gggtggcatg gagccggggg tcatcccgcc gatgtccgcg tgatgggcgc
      241 gcgaggcgac gaagaaatcc acgcgctcgg cgtcggcgac gaacaccggg gtgacgaccg
      301 tcatgtcggg cagatgggtg ccgccgtgat acgggctgtt gaggagccag gaatcgccgg
      361 gcctgagctg cgcagcccgc gtctcgatga ccgcccgtac gctcgcaccc atcgagccca
      421 ggtgcaccgg catgtgcggc gcgttggcga ccagcccccc gcgtgagtcg aacagcgcgc
      481 aagagtagtc gagccgctcg cggatgttga cggactgggc ggtcgccttg agtacctccc
      541 ccatctgctc ggcgatgtgc atgaagaggt tgttggatat ctcgatccgc gcggggtcgg
      601 tcgcggcggc ggcgggcgcg gccggtggag ccggctcgga ctcgagcagc agctccccgc
      661 ccgcaagcgc gctggcgcgc caaccgttct ccaccaccac ggtgctgtgc ggctcgacga
      721 tgagcgcagg tcctgcgagc gtgccctcga gctcgcccat tggcacgagc gggacctctc
      781 gccatccgtc gaaccaggcg cgtaccgtac gcggcggcgt agcgcgggtg cgcggcgcgg
      841 gcatggcgag gcgcgcgccg tcgagcgatg ccaggcgcgc ctcggcgcgc aacgcctcga
      901 tcacgacgtc gagggtatgc gcggcgaagc cgaagcgctt caggtgctcg gcgtggaagc
      961 gctcgtgcac ctcggcgagg gagccgagtg gcacggacag cgtcacgtcg ctgtcgccgg
     1021 ctcgcacctc gaggaacagc gccacccgcg cgcgccccgg ctcggccccg aaggcggcga
     1081 gctcctcgcg ggcctgcgac tcgaggagcg cgagcgccgc gcgcgccgcg gcgagccctt
     1141 gggagctaag cccctggcgc aggctcgcgc gccgcacggc caggcgatcg gccactccga
     1201 tgccgaatgc cgagagcacg ctcgccaggg ggtgtaccag gatgcggcgc atgccggcgg
     1261 cgcgcgccac gcggcaggcg tgctggcccg ccgcgccgcc gaaacagaac agcgtgaagt
     1321 ctcccgcgtc gagtccctgc cgcgccgaca cctggcggat ggcgttcgcc atcgactcga
     1381 caccgacttc gaggaacgcc tccgcgacca cctccgccgg gcagtgctgg gcgcccgcag
     1441 cgctggcgcg cgccgcgagc tcggcaaagg cggccgcgac cacgtccggg tcgatacgcg
     1501 atcgaccgtc ctgcccgaac accgccggca atgtgtcggg ccgcaggtgc ccgagcagca
     1561 cctggacgtc ggtgagagtc agcggcccgc cgcggcggta gcaggccggt ccgggttccg
     1621 cacccgctga ggcgggtccc accgcgaagc gcccgtccct gaagctgaga atcgagccgc
     1681 cgcccgcggc gatcgtgtgc acatcgagca tcggctgcat gaggcggacg cccgcgatcg
     1741 tgtgctcgaa gcgctgcggc agctcgccgt cgatcagcga cacgtcggtc gacgtcccgc
     1801 ccatgtcaaa gccgatgagc ttcgcgaagc ccgcccgctc gcccacccag cgcatgccga
     1861 tgagcccgcc ggccggtccc gacaacacgc tcgacatggc gtggaaggcc gccgcgtccg
     1921 ccagtccgcc gttgctctgc atcagcgtca ggcgcgagcg cgcatcgagc gcgtgcagct
     1981 gtgccttcag cccgcccagg taggcgcgca gcggcagcgc cagataggcg ttcagcacag
     2041 tcgtgtcggc gcgcgtgacg tagcgcacga ggggcgagag ctcgtgcgac accgagacct
     2101 cctcgaagcc aagctcgcgg gccgtggcgg cggcgcggcg ctcatgctgc ggatggcgcc
     2161 agccgtgcac gaacacgatg gccacggcgc ggcgcccggc gcggcgcgcc tgcgccagct
     2221 ccgcccgcaa gcgcttctca tcgaggggc
//
LOCUS       DATLKL010000175         2845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_114858, whole genome shotgun sequence.
ACCESSION   DATLKL010000175 DATLKL010000000
VERSION     DATLKL010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114858"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VM713_02300"
     CDS             <1..899
                     /locus_tag="VM713_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HVH83124.1"
                     /translation="TAGNDEQVSLTLAYRPPYDWGYVSAFLGRRAICGVEEVSASGYA
                     RTIRLARGHARLHVAPMEGEHALRLQVSCAEPGDLFEIATTARRVFDLTADPAAIAAV
                     LSADPVLRPLVIAGPGLRIPGVWDAFECAVRGVLGERLSVRAARRLTERLVQRFGERV
                     AAGAHGLTHVFPSAARLAEADLTTIGLTPTRSAAVRALARAVRDRSLNLSAHAEEVVR
                     ALGAVPGVSAWVVQYVALRALGEPDAFPGGDRWLRRLSRARRSPPRTVAGLERRARAW
                     RPWRGYATLYLWRRADGDAAPR"
     gene            960..>2845
                     /gene="ppk1"
                     /locus_tag="VM713_02305"
     CDS             960..>2845
                     /gene="ppk1"
                     /locus_tag="VM713_02305"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HVH83125.1"
                     /translation="MDAPDLKAPQHYINRELSFLEFNQRVLDQAFDESVPLLERLRFL
                     CISSSNLDEFFEIRVAGLKQLTELGGGQLAPDGMSIPEQLAAIHDRATRLVADQYRCL
                     NELLLPALAREGAPLLERRDWSPAAHQWLEEYFEREVEPVLSPLGLDPSRPFPRIQNK
                     SLNFIVRLEGKDAFNRNSELAIVQAPRSLPRVVPLPQEGTGRACVLLSTIVQTFVPKL
                     FTGIQVIGCYQFRVTRNSDLFVDEEEIDDLRRALEGELIHRRYGAAVRLEVASECPDD
                     MVKFLHSQFGSNGLDTYYVPGPVNLNRLTAVYDLVQRPDLKYPIFTPGLPKRLVGATD
                     LFAIIRQKDVLLHHPYQSFAPVMDFLRQAAADPQVLAIKQTLYRAGHDSPLVDALVAA
                     ANAGKDVTVIVELRARFDEEANIELSNRLQEAGAHVMYGVVGYKTHAKLLLVVRREQG
                     GIRRYCHLGTGNYHPRTARQYTDYGLFTCDAEIGQDVHELFLQLTSLTQTPKLNRLTQ
                     SPFGMHDMVLGKLARESEHARAGRPARVIAKMNALVDPQTIEALYRASNAGVKIDLIV
                     RGVCALRPGVPGVSENITVRSIVGRFLEHSRVFYFENAGEPEILCASADWMERNFFRR
                     VEVT"
BASE COUNT          423 a          982 c          975 g          465 t
ORIGIN      
        1 tcaccgccgg aaacgacgag caggtctccc tgacgctggc ctaccgacct ccgtacgact
       61 ggggctatgt gagcgcattt ctcggccgcc gggccatctg tggagtcgag gaggtcagcg
      121 ccagcggcta cgcccgtacc attcggctcg cacgcgggca tgcgcggctg cacgtggccc
      181 cgatggaagg cgagcacgcc ctgaggctgc aggtatcgtg cgccgagccc ggcgacctgt
      241 ttgagatcgc gaccacagcc cggcgcgtgt tcgatctcac ggccgacccg gctgcgatcg
      301 ccgcggtcct gtcggcggat cccgtgttgc gaccgctggt gatcgcgggg ccggggctgc
      361 gaataccggg cgtgtgggat gcgttcgagt gtgccgtgcg cggggtgctc ggggagcgcc
      421 tgagcgtccg cgccgcgcgg cgcctgaccg agcggctcgt gcagcgattc ggggagcgcg
      481 tcgccgcggg agcccatggg ctgacgcatg tgttcccgtc ggccgcgagg ctcgcggaag
      541 cggacctgac cacgatcggt ctcacgccca cgcgcagcgc cgcggtgcgt gccctggcac
      601 gcgccgttcg cgaccgcagt ttgaatctga gcgcgcacgc tgaggaagtg gtcagggcgc
      661 tcggcgcggt tcccggcgtg tcggcatggg tcgtgcaata tgtggcgctg cgcgcgctgg
      721 gcgagccgga cgcttttccc ggcggcgatc ggtggctacg gcggttgagc agagcccgcc
      781 gctccccgcc tcggacggtg gccgggctcg agaggcgcgc cagggcctgg cgtccgtgga
      841 ggggctacgc gacgctttac ctgtggaggc gcgccgacgg ggacgcggcg ccccgctgag
      901 atccgccccg catgcttctt cacggaagcg tcgcgcgcgg ctgatatgct tagaggttca
      961 tggacgctcc cgacctcaag gcgccgcagc actacatcaa ccgggagctc tcgttcctcg
     1021 agttcaatca gcgcgtcctc gaccaggcct tcgacgagtc cgtgccgctc ctcgagcggt
     1081 tgcgcttcct gtgcatctcg tcgagcaatc tcgacgagtt cttcgagatc cgcgtcgccg
     1141 gcctcaagca gctgaccgag ctcggcggcg gccagctcgc ccccgatggc atgtcgatcc
     1201 cggagcagct cgccgcgatt cacgaccgcg cgacgcggct cgtggcggac cagtaccgct
     1261 gcctcaatga gctgttactt ccagcgctcg cccgcgaggg tgcaccgctc ctcgagcgcc
     1321 gggactggag cccggccgct caccagtggc tcgaggagta cttcgagcgc gaggtggaac
     1381 cggtgctctc gcccttggga ctggatccct cgcgcccctt tccgcgtatc cagaacaaga
     1441 gcctgaactt catcgtccgg ctcgagggga aggatgcctt caaccgcaac agcgagctcg
     1501 cgatcgtgca ggcgccgcgg tcgctgccgc gcgtcgtgcc gctgccgcag gagggcaccg
     1561 ggcgcgcctg cgtgctgctc tcgaccatcg tgcagacctt cgtgccgaag ctgttcaccg
     1621 gcattcaggt gatcggctgc taccagttcc gtgtgacgcg caacagcgat ctgttcgtcg
     1681 acgaggagga aatcgacgat ctgcggcgcg cgctcgaggg cgagctcatt caccgccgct
     1741 acggcgcggc cgtgcggctc gaggtcgcga gcgagtgccc ggacgacatg gtgaagttcc
     1801 tgcactcgca gttcggctcg aatggtctcg acacctacta cgtgccgggg ccggtgaacc
     1861 tcaaccgcct cacggcggtg tacgacctgg tgcagcgccc ggacctcaag tacccgatct
     1921 tcacgccggg gctgccgaag cggctcgttg gggccacgga cctgttcgcc atcatccgcc
     1981 agaaggacgt gctgcttcac cacccgtacc agagcttcgc gccggtcatg gatttcctgc
     2041 ggcaggccgc ggccgatccg caggtgctcg ccatcaaaca gacgctctac cgcgccggcc
     2101 acgactcccc gctggtcgat gcgctggtgg ccgcggccaa tgccggcaag gacgtgacgg
     2161 tgatcgtcga gctgcgggcc cgcttcgacg aggaggccaa catcgagctc tccaaccgcc
     2221 tgcaggaggc gggcgcgcac gtcatgtacg gcgtggtggg ctacaagacg cacgccaagc
     2281 tgctgctggt cgtgcgccgc gagcagggcg gcatccgccg ctactgccac ctgggcacgg
     2341 gcaactacca cccgcgcacg gcgcggcagt acaccgacta cgggctcttc acctgcgacg
     2401 ccgagatcgg ccaggacgtg cacgagctgt tcctgcagct caccagcctc acacagaccc
     2461 cgaagctgaa ccggctgacg cagtccccat tcggcatgca cgacatggtg ctcggcaaac
     2521 tcgcgcgcga gtcggagcac gcgcgtgcgg gccgcccggc gcgggtgatc gccaagatga
     2581 acgctctggt ggatccacag accatcgagg cgctctaccg cgcctccaac gccggggtga
     2641 agatcgatct gatcgtgcgc ggtgtgtgtg cgctccgccc gggggtcccg ggggtgtcgg
     2701 agaacatcac cgtgcgctcg atcgtcggcc gcttcctcga gcactcacgg gtgttctact
     2761 tcgagaacgc cggtgagccg gagattttgt gcgccagcgc cgactggatg gagcgcaact
     2821 tcttccgccg cgtcgaagtc acctt
//
LOCUS       DATLKL010000176         2272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_47142, whole genome shotgun sequence.
ACCESSION   DATLKL010000176 DATLKL010000000
VERSION     DATLKL010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47142"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VM713_02310"
     CDS             <1..532
                     /locus_tag="VM713_02310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HVH83126.1"
                     /translation="ASGLAVAFIEETFLRGAMYTAIARESGPRAAVLLTALLYAATHF
                     FGKVRIAPQDVTPWSGLALLGQSLQAFAHFAGIADAFLALLAVGVVLALVRQATGNIA
                     ACLGLHAGWVWVMLVAHQLTTPRREASLGFLLSRFDGFVGWLVLGWTVLLAWPLWRFY
                     ASRTAVIAKKTFAGTP"
     gene            complement(578..1447)
                     /locus_tag="VM713_02315"
     CDS             complement(578..1447)
                     /locus_tag="VM713_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HVH83127.1"
                     /translation="MAGSACRWLLALGLLGCLSAEAASPVWAIRGAHNTLYLAGSIHL
                     LPEADAGLPQAFARAYGDSARLVMEIDLGSLDPGEALGTLLQHGGLAAGESLREVVGA
                     ARYARASAAVAALGLPAASIDHEAPWMVALQLTELEYARLGFDPERGVEQQLLRRARA
                     DHKTTAGLESLADEMGVFEALTPAAQLRLLDMVLDDLDDAGNETHAVLAAWRRGDTAQ
                     LAALLAAEYRTFPALYDALVMDRNRHWLPEIEQLLKGGDNCLVVVGALHLVGKGGLLE
                     ALRHDGFTVEQLN"
     gene            complement(1393..2184)
                     /locus_tag="VM713_02320"
     CDS             complement(1393..2184)
                     /locus_tag="VM713_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVH83128.1"
                     /translation="MSVVRITQFTDPHLYGAETGTLRGVATLPALTAALARARSHDWP
                     PDAVLVTGDIVQDDPGGYPHFRRVLGGLSVPVLCIPGNHDEPEAMRRELAGPPFVLGG
                     HVDLGRWRIVLLDSCLAGSASGRLSPEALSGLDAALRSAGKRHCMVCLHHHPVPMASR
                     WLDRVGLENAPEFLAAIDRHPNVRAVVWGHVHQTYDALREGVRLLATPSTCAQFLPNS
                     DDFALDPRPPAYRTLELRADGSLLTEVVWLDLRAGGSSRSAYSAA"
BASE COUNT          334 a          790 c          811 g          337 t
ORIGIN      
        1 cgccagcgga ctcgccgtgg cattcataga ggagaccttc ctgcgaggcg cgatgtacac
       61 ggccatcgcg cgagagtcgg ggccgcgcgc cgccgtgctg ctcacggcgc tcctttacgc
      121 ggcaacacat ttcttcggca aggtgcgcat cgcgccgcag gacgtgacgc cctggagcgg
      181 gcttgcgctg ctcgggcagt cactgcaggc gttcgcgcac ttcgcgggga tcgccgacgc
      241 gttcttggcg ctgctggcgg tcggcgtggt gctggccctc gtgcggcagg cgaccggcaa
      301 catcgctgcc tgcctcgggc tgcacgccgg ctgggtgtgg gtcatgctcg tggctcacca
      361 gctgactacg ccgcgccgcg aggcgtcgct cggctttctc cttagccgct tcgatggctt
      421 cgtcggctgg ctggtgctcg gctggacggt gctgctggcc tggccgttgt ggcgtttcta
      481 cgcctcgcgc accgcggtga tcgcaaaaaa gacatttgcg gggacgccgt gaatccatcc
      541 atggaggctg cgctcgccgc ggtccccccg gcgatgttca attcagctgc tcgaccgtga
      601 agccgtcgtg gcgcagcgcc tcgaggagtc cccccttgcc gaccaggtgc agggctccga
      661 ccacgaccag gcagttgtcc ccgcccttca gcagctgctc gatctcgggg agccagtgcc
      721 ggttgcgatc catcaccagg gcgtcgtaga gcgccgggaa ggtgcggtat tcggccgcaa
      781 gcagcgcggc gagctgcgcg gtgtcaccgc gccgccaggc cgcgagcacc gcgtgtgtct
      841 cgttcccggc atcgtcgaga tcgtcgagca ccatgtcgag gaggcgcagc tgcgcggccg
      901 gcgtgagcgc ctcgaagacg cccatctcgt ccgcgaggct ctcgagtccg gcggtagtct
      961 tgtgatcggc gcgcgcacgg cgcagcagct gttgctcaac tccccgctcc gggtcgaagc
     1021 cgaggcgtgc gtactcgagc tcggtgagct gcagggcgac catccacggc gcctcgtgat
     1081 cgatcgaggc ggccggcagc ccgagcgccg cgaccgcggc gctggcccgc gcgtagcgcg
     1141 cggcgccgac gacttcacgc aggctctcgc ctgcggcgag accgccgtgc tgcagcagcg
     1201 tgccgagcgc ctcgcccgga tcgagcgagc ctaagtcgat ctccatcacc aggcgcgccg
     1261 agtcgccgta ggcgcgcgcg aaggcctgcg gcaggccggc atcggcctcc ggcagcaggt
     1321 gaatcgagcc tgcgaggtag agcgtgttat gtgcgccgcg gatcgcccac accgggctcg
     1381 cggcttcggc gctcaggcag ccgagtaggc cgagcgcgag gagccaccgg cacgcagatc
     1441 cagccacacg acctccgtaa gcagcgagcc gtcggcacgc agctcgaggg tacgatacgc
     1501 cggtggccgc gggtccagcg cgaagtcgtc cgagttgggc aggaactgcg cgcaggtcga
     1561 gggcgtagcc agcagccgca ctccctcgcg cagggcgtcg taggtctgat gtacgtgacc
     1621 ccacacgacg gcgcgcacgt tggggtgccg atcgatcgcg gcgaggaatt ccggcgcgtt
     1681 ctcgagtccc acgcggtcga gccaacggct cgccatcggc accggatgat gatgcaggca
     1741 caccatgcaa tggcgcttgc cggcgctgcg cagcgccgcg tcgagtccgc tgagcgcctc
     1801 agggctcaac cgcccgctcg ccgagccggc caggcagctg tcgaggagca cgatgcgcca
     1861 gcggccgaga tccacgtggc cgccgaggac aaacggcggc ccggcgagct cgcggcgcat
     1921 cgcctccggc tcgtcgtgat tgccgggaat gcacagtacg ggaacgctga gcccgccgag
     1981 cacgcggcgg aagtgcgggt acccgcccgg atcgtcctgc acgatgtcgc cggtcaccag
     2041 caccgcgtcc ggcggccagt cgtgcgagcg cgcacgcgca agcgcagccg tgagcgcggg
     2101 cagagtcgcc acgcctcgca gcgtcccggt ctcagccccg tacagatgcg gatcggtgaa
     2161 ttgtgtgatg cgaacgacgc tcatcagtaa agccagccta gcagggccgg cccggagagg
     2221 cagtgaacca ggtcgccggc cgacgggccg cgggtgtcgc ctagtgtcta ca
//
LOCUS       DATLKL010000177         2639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_92373, whole genome shotgun sequence.
ACCESSION   DATLKL010000177 DATLKL010000000
VERSION     DATLKL010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92373"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VM713_02325"
     CDS             <1..708
                     /locus_tag="VM713_02325"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVH83129.1"
                     /translation="GRTEGGACGLAARRGRAPTLSGPAPVATGARTAAILEWARKVQA
                     IAQNGLAFSRDPFDRARYQELEQLTATMLSTELEAPLATVRAFWQGEHGYATPKVDVR
                     GGVFQGAEVLLVRERADGRWTLPGGWVDVNDAPSAAVAREIFEESGYQARAVKLAALV
                     DKNRHPHPPGVHHIYKLFFLCELTGGAPATSAETDAVGFFPVTALPELSTGRVLATQI
                     ERLYQHQLDRSLPTDFD"
     gene            724..2430
                     /gene="aceK"
                     /locus_tag="VM713_02330"
     CDS             724..2430
                     /gene="aceK"
                     /locus_tag="VM713_02330"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HVH83130.1"
                     /translation="MDCARHLVEAFANYNAEFRAITRRAPLRFDGRDWKGSQRDAVER
                     IELYDRFVDETIAELRTALGGEALDRERWQRIRHEFAALIADLPDPEFTKTFFSSVTR
                     RLFGTVGVAPDIEFVATDLDPLADIHSSVGTNTYLNHGSLPLLFEDLLGDVRFRSPWR
                     DLDKSIAHVAAEVRTYLEAKDEWRAVERVEVIRPVFYQVTRAFIVGRIVGPELLVPLV
                     IALKNTDGGMLVDAVMLAEDDVSIVFSFTRSYFHVDLERVAEAVVFLRSIMPKKPVSE
                     LFTVLGRARQGKTERYRELVRHLEGTEDLFAHAPGERGLVMVCFTLPSFDVVFKVIRD
                     RFAPPKTVLREEVMAKYQMVFVHDRAGRLVDAQEFRRLRFPRSRFAAQLLEELEREAA
                     HSVHADGADLVFDHMYVERRMTPLNLYLREAGPAAAVRAALDYGQCIRDLACTNIFPG
                     DLLLKNFGVTRHGRVIFYDYDELCRVTDCNFRDLPEAQNAEDEMRGEAWFYVAENDVF
                     PETFMRFLAFGDDERAAVLRVHAEILTAKFWRDLQQRLREGEVVEVLPYHAHRVRVAS
                     SG"
BASE COUNT          419 a          886 c          900 g          434 t
ORIGIN      
        1 ggtcgtacgg aaggcggcgc gtgcggactg gccgcgcgtc gcggccgcgc gccgaccttg
       61 agcggaccgg ctccggtggc taccggcgcg cgtactgcag cgatcctcga gtgggcgcgc
      121 aaggtgcagg cgatcgccca gaacggcctc gccttcagcc gcgacccctt cgaccgcgca
      181 cgttaccagg agctcgagca gctcaccgcg acgatgctca gcaccgagct cgaggcgccg
      241 ctcgccacgg tgcgcgcctt ctggcagggc gagcacggct acgcgacgcc gaaggtggat
      301 gtgcgcggcg gggtgttcca gggcgccgag gtgctgctgg tgcgcgagcg ggccgacggg
      361 cgctggacgc tcccaggagg ctgggtcgac gtgaacgatg cgccctccgc cgccgtagcg
      421 cgggagatct tcgaggaatc cggctatcag gcgcgcgcgg taaagctcgc cgccctcgtc
      481 gacaagaacc gccacccgca tccgcccgga gtacaccata tctacaagct cttttttctg
      541 tgcgagctca ccggcggcgc ccccgccacg agcgcggaga ccgacgccgt ggggttcttc
      601 cccgtgacgg cgctgccgga gctgtcgacc ggccgggtgc tcgccacgca gatcgagcgc
      661 ctttatcagc accagctcga ccgctcgctg ccgaccgact tcgattgatg gcacgctcag
      721 tccgtggatt gcgcacgtca cctcgtcgaa gccttcgcca actacaacgc cgagttccgt
      781 gccatcacgc gtcgtgcccc gctgcgtttc gacggccgcg actggaaggg cagccagcgc
      841 gacgcggtcg agcgcatcga gctctatgac cgtttcgtgg acgagacgat cgcggagctg
      901 cgcaccgccc tcggcggtga ggcgctcgat cgcgagcgct ggcaacggat ccggcacgag
      961 ttcgccgcac tgatcgctga tctgccggac ccggagttca ccaagacctt cttcagctcg
     1021 gtcacccgcc gcctgttcgg caccgtaggg gtcgccccgg acatcgagtt cgtcgcgacc
     1081 gatctcgatc cgctcgccga catccactcg agcgtcggca caaacaccta cttgaatcac
     1141 gggtcgctgc cgctgctgtt cgaggacctg ctgggggacg tgcgcttccg ctcgccgtgg
     1201 cgcgacctcg acaagagcat cgcgcacgtc gccgccgagg tacgtaccta cctcgaggcg
     1261 aaagacgagt ggcgcgcggt cgagcgggtg gaggtcattc gccccgtgtt ctaccaggtg
     1321 acgcgcgcct tcatcgtcgg ccgcatcgtc ggcccggaat tgctggtgcc gctggtgatc
     1381 gccctcaaga acaccgacgg cggcatgctg gtcgatgccg tgatgctggc cgaggatgac
     1441 gtcagcatcg tcttcagctt cacgcgctct tacttccatg tcgacctcga gcgggtcgcg
     1501 gaagcggtgg tgttcctgcg atcgatcatg ccgaagaagc ccgtgagcga gctgttcacc
     1561 gtgctggggc gcgcacggca gggaaagacg gagcgctacc gggaactggt gcgccacctc
     1621 gagggcaccg aggacctgtt cgcgcacgca ccgggtgagc gggggctcgt catggtgtgc
     1681 ttcacgctcc cctccttcga cgtcgtcttc aaggtgatcc gcgaccgttt tgccccgccc
     1741 aagacggtgc tgcgcgagga ggtgatggcg aagtaccaga tggtcttcgt ccacgaccgc
     1801 gccggacggc tggtggacgc gcaggaattc cgccgcctgc gatttccgcg ctcgcgcttc
     1861 gcagcccagc tgctcgagga gctcgagcgc gaggctgccc acagcgtgca cgcggacggc
     1921 gccgatctgg tgttcgacca catgtacgtc gagcggcgca tgacgccgct caacctgtat
     1981 ctgcgtgagg cgggcccagc ggcggcggtg cgggccgcgc tcgattacgg ccagtgcatc
     2041 cgcgacctgg cctgtaccaa tatctttccc ggcgatctgc tgctgaagaa cttcggcgtc
     2101 acgcgccacg ggcgcgtgat cttctacgac tacgacgagc tgtgccgggt gaccgactgc
     2161 aacttccgcg atctgcccga ggcgcagaac gccgaggacg agatgcgcgg cgaagcctgg
     2221 ttctacgtgg ccgagaacga cgtctttccc gagaccttca tgcgcttcct cgccttcggc
     2281 gacgacgagc gcgcggcggt gctgcgcgtg cacgcggaga tcctcaccgc gaagttctgg
     2341 cgcgacctgc agcagcggct gcgcgagggt gaggtggtcg aggtactgcc ctaccacgcc
     2401 caccgcgtgc gggtcgcgag cagcgggtga gcggccggcg ggcaggccgg aggctttgct
     2461 gaaacactca ggggaccggt catgactttc caatacatag acgttgaaga ggcgaggaaa
     2521 cgcaacggcc tgcgcatggt ggtcgtgggt aatgtgccca gcggctggag cgaagccgcc
     2581 aagggcgtcc tctacatcaa gggcatcgaa tgggccgcgg tacggctggt ctacgacag
//
LOCUS       DATLKL010000178         1796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_92393, whole genome shotgun sequence.
ACCESSION   DATLKL010000178 DATLKL010000000
VERSION     DATLKL010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92393"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="VM713_02335"
     CDS             complement(<1..1757)
                     /locus_tag="VM713_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVH83131.1"
                     /translation="MIDAVVHLEIKFVQLEARVGIATGLVVVGDLIGAGSAQEQSVVG
                     ETPNLAARLQTLAEPGTVVIAASTRRLVADLFEYRDLGAVEVKGIAAPVPAWQVLGPS
                     SVASRFEALRGAALSRLIGRDEEVDLLLRRWARAKAGDGQIVLISGEPGIGKSRITVE
                     LEERLRPEPHLHLRYFCSPYCRDSALYPFIDQLWHAAEFARDDPPTAKLEKLEAVLFR
                     AAQPDEDVPLLADLMSLPVSARHPLPNLNPQRKKERTLEVLLRQLEGLARQKPVLMVF
                     EDAHWIDPTSRELLDLTVERVSSLPVLLIATFRPEFQMPWTGQPQVTMLALNRLDRRD
                     RTVLVEQIAGGKTLPDEVVAQIADRTDGVPLFVEELAKSVLESGVPLHSIPSSLHDSL
                     MARLDRFASVRHVAQIGAAIGRQFPYALLRAVSRLPEDELRAALAQLVASELVFQRGT
                     PPDAVYTFKHALVRDAAYASLPRSQRQLLHARIVSTLEAHFPEVVETQPELLAHHCVE
                     GGLIAKATGYWLKAGQRAAQRSASTEAIAHFAKGIEALATLPDTTQREQQELTFRLAL
                     GPVLIATRGWNSIDAETSY"
BASE COUNT          330 a          587 c          587 g          292 t
ORIGIN      
        1 tcataggaag tttcagcatc gatgctgttc cagccgcgcg tcgcgatgag aaccggccct
       61 agcgccaatc ggaaggtcag ctcctgctgc tcgcgctgag tggtatcggg aagggtcgca
      121 agggcctcga tgcccttggc aaagtgtgcg atcgcttcgg tcgatgcgga acgctgcgcg
      181 gcgcgctgtc ccgctttgag ccaatagccc gttgccttgg cgatgaggcc tccctcgacg
      241 cagtggtgcg caagcaactc tggttgcgtc tcgacgactt ccggaaagtg cgcttcgagc
      301 gtggacacga tgcgagcgtg aagcagctgg cgctggcttc gcggcaaact ggcataggcg
      361 gcgtcgcgca ccagcgcgtg cttgaaagta tagaccgcgt cgggcggcgt gccgcgctgg
      421 aacaccagct cggaggcgac aagctgggcg agcgcggccc gcaactcgtc ctcggggaga
      481 cgggagacgg cgcgcagcag cgcataagga aactggcgtc caatcgcagc accgatctgc
      541 gccacatgcc gcaccgaggc aaaacggtcg agccgcgcca tcagcgaatc gtgcaggctt
      601 gatggaatac tgtgcagcgg cacgccgctc tccagaacgc tcttggccaa ttcctcgaca
      661 aacaatggca cgccgtcggt gcggtcggcg atctgggcga cgacctcgtc gggcaacgtc
      721 tttccgccgg caatctgctc gaccaggaca gtccggtcgc gccgatccag gcgattgagc
      781 gccaacatcg tcacctgcgg ctggccggtc cagggcatct ggaactccgg gcggaacgtc
      841 gcgatcagca gcaccggcag gctgctaacc cgctcgaccg tgaggtcgag gagttcacgc
      901 gaggtcgggt cgatccaatg cgcgtcctcg aagaccatca gcaccggctt ctggcgcgcc
      961 agaccttcga gctggcggag cagcacctcc agcgtccgct ccttcttacg ctgcggattg
     1021 aggttgggca gcgggtggcg cgccgaaacg ggcaatgaca tgagatcggc gaggagtggc
     1081 acatcctcgt ccggctgtgc tgcgcggaac agcacggctt ctagcttttc taacttggcc
     1141 gtaggcgggt cgtcgcgtgc aaactcagca gcatgccaaa gctggtcgat aaacggatag
     1201 agcgcgctgt cacggcaata gggcgagcag aaatagcgca gatggagatg cggctccggg
     1261 cgaaggcgct cctctaactc cacggtgata cgcgacttgc cgatgccggg ctcgcccgag
     1321 atcagaacga tctggccgtc cccggcctta gcgcgcgccc agcggcgcaa taacagatcg
     1381 acctcttcgt cgcggccgat cagccggctc aaggccgctc cgcgtaaggc ctcgaagcgg
     1441 ctcgccacgc tgctcgggcc cagcacctgc caagccggca ccggcgcggc gatacccttc
     1501 acctcgacgg cgccgagatc gcggtactcg aagagatcgg cgaccagccg gcgcgtgctc
     1561 gcggcgatca cgaccgtgcc tggctcggct agcgtttgca gccgagcggc tagattgggt
     1621 gtttcgccga cgactgactg ctcctgcgcc gatcccgcgc cgatcagatc gccgacgacc
     1681 accagcccgg tggcgatccc gacacgggcc tcgagctgga cgaatttgat ttcaaggtga
     1741 acgactgcgt cgatcacacc aagcccggcg cggaccgccc tctcggcatc atcctc
//
LOCUS       DATLKL010000179         1728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_137553, whole genome shotgun sequence.
ACCESSION   DATLKL010000179 DATLKL010000000
VERSION     DATLKL010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137553"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /gene="ftsW"
                     /locus_tag="VM713_02340"
     CDS             <1..1077
                     /gene="ftsW"
                     /locus_tag="VM713_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HVH83132.1"
                     /translation="SVSVASRETGQPFYYLERQLALVAVGTVCAAFVFSLRTEWLERA
                     SGPLLAVALVLLVLVLLPGLGHAVNGSRRWLHLAAVNFQVSELARVLVLVYVASYAVR
                     REEQLRTSFTGLAKPLALLALAAALLLVEPDFGAATVLFAGGFGLLFLAGARLRYVLA
                     MMALAAGGFALLALGSSYRLRRLTTFLDPWADPYHSGFQLTQSLIAIGRGQWLGVGLG
                     ESVQKLFYLPEAHTDFLFAVLSEELGLIGVVLTLTLFLILIWRAFYIARLASDAGLKF
                     PAYLAAGFGLWVGIQTFINVGVNMGVLPTKGLTLPLMSYGRSSLVVTLAWVGLLLRVY
                     HEAMREGRGSATVRAPDGQREGLW"
     gene            1074..>1728
                     /locus_tag="VM713_02345"
     CDS             1074..>1728
                     /locus_tag="VM713_02345"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HVH83133.1"
                     /translation="MSRPILIMAGGTGGHVFPALALARLLRERAFEVVWFGTDRGLEA
                     RLIPAEGIPLERLSVAGLRGKGVLPWLAAPFRLTRALVQALRIMRRHQPLVVVGLGGF
                     VTGPGGVAAWLTRRPLVIHEQNAVAGFTNRILAHLARVVLEAFPDSFGRDVHARLIGN
                     PVRRDISAVAAPHERFTGRSGPIRLLVFGGSQGAARLNAVVPFALARLAGRLAFDVRH
                     "
BASE COUNT          183 a          615 c          607 g          323 t
ORIGIN      
        1 tccgtctcgg tcgcgagccg cgagacgggc cagcccttct actacctcga gcggcagctg
       61 gcgctggtcg ccgtgggcac ggtgtgtgcg gcgttcgtct tctcactgcg caccgagtgg
      121 ctggagcgcg cctcgggccc gttgctcgcc gtcgcgctgg tgctgctggt gctggtgctg
      181 ttgcccggac tcgggcacgc ggtcaacggc agccgccgct ggctgcacct ggccgccgtc
      241 aacttccagg tgtcggagct cgcgcgggtg ctggtgctgg tgtacgtcgc gagctatgcc
      301 gtgaggcggg aggagcagct gcgcacgagc ttcacggggc tcgcgaagcc gctcgcgctg
      361 ctcgcgctcg ccgcggcgct gctgctcgtc gagccggact tcggcgccgc caccgtgctg
      421 ttcgccggcg gctttgggct gctgttcctc gccggcgcgc ggctgcgcta cgtgctcgcc
      481 atgatggcgc tcgctgcggg cggtttcgcg ctgctcgccc tcggctcgag ttaccgcctg
      541 cgccgcctca ccaccttcct cgatccctgg gccgatcctt accacagcgg cttccagctc
      601 acccagtcgc tgatcgccat cgggcgcggc cagtggctcg gcgtcggtct cggcgagagc
      661 gtgcagaaac tgttttatct ccccgaggcc cacaccgact tcctgttcgc cgtactctcg
      721 gaggagctcg gacttatcgg cgtggtgctg acgctcacgc tctttctcat cctcatctgg
      781 cgcgccttct acatcgcgag gctcgcctcc gatgcgggac tgaagttccc ggcctacctc
      841 gccgcgggct ttggtctctg ggtcggcatc cagaccttca tcaacgtcgg cgtcaacatg
      901 ggcgtgctgc cgaccaaggg cctgacgctg ccgctcatga gctacgggcg ctcgagcctg
      961 gtggtgacgc tcgcctgggt ggggctgctg ctgcgcgtct accatgaggc aatgcgcgag
     1021 gggcgcggct cggccaccgt gcgtgcgccc gacgggcagc gggaggggct gtggtgagcc
     1081 gcccgatcct catcatggcg ggcggcaccg gcgggcatgt gtttccggca ctggcgctgg
     1141 cgcgccttct gcgcgagagg gccttcgagg tcgtgtggtt cggcaccgat cgcgggctcg
     1201 aggcccgtct catccccgcc gagggcatcc ctctcgagcg gctgtcggtc gcgggactgc
     1261 gcggcaaggg tgtgctcccc tggctcgccg ccccgtttcg cctgacgcgg gcgctggtgc
     1321 aggcactcag gatcatgcgg cgccatcagc cgctggtcgt ggtcggcctc ggcggcttcg
     1381 tcaccggtcc cggcggggtc gccgcctggc tgacgcgccg gccgctcgtc atccacgaac
     1441 agaacgcggt cgccggcttc accaaccgga tcctcgcaca cctcgcgcgc gtggtgctcg
     1501 aggcctttcc cgacagcttc gggcgcgacg tgcacgctcg cctgatcggc aatccggtgc
     1561 ggcgcgacat cagtgcggtg gcggcgccgc acgaaagatt cacgggtcgc tcggggccca
     1621 tacgcctcct ggtgttcggc ggcagccagg gggcggcgcg cctaaatgcg gtcgtgccgt
     1681 tcgcgctagc tcgcctcgcc ggcaggctcg ctttcgacgt gcgtcacc
//
LOCUS       DATLKL010000180         2282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_47229, whole genome shotgun sequence.
ACCESSION   DATLKL010000180 DATLKL010000000
VERSION     DATLKL010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47229"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2132)
                     /locus_tag="VM713_02350"
     CDS             complement(<1..2132)
                     /locus_tag="VM713_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVH83134.1"
                     /translation="MYAIDYEDFGTPPPADASGSVTVEIDGRTVTVPDGTSIMRAAAL
                     AGVRVPKLCATDTLKAFGSCRLCLCEIDGRKGYPASCTTTVAAGMKVRTESPKLKQLR
                     RSVMELYLSDYPIERQPTEAEARTELLAMAEKLGVRSTPYANGKRHQGAPKDESNPYF
                     TFDPNLCIVCSRCVRACDETQGTFALTIQGRGFASKVAASQDQPFLESECVSCGACVE
                     ACPTAALTEKSIIGLGAPERAEKTTCAYCGVGCGFTAEMRGEGADAEVVRMVPDRDGG
                     ANHGHACVKGRFAWGYATHPDRQLQPMIRKTIRDNWREVSWEEAINYAASEFKRIQAK
                     YGRGSVGGITSSRCTNEETFLVQKLVRAAFGNNNVDTCARVCHSPTGYGLKNTLGESA
                     GTQAFDSVMKADVIMVIGANPTDGHPVFASQMKRRLRQGAKLIIADPRQIGLVHSPHV
                     EADEHLQLKPGTNVALLNALAHVVVTEGLAKEAYVAERCEPESWAKWKAFVAEARNSP
                     EASESITGVPAERVRRAARMYAQAGNAAIYYGLGVTEHSQGTTAVMGIANLAMATGNL
                     GREGVGVSPLRGQNNVQGSCDMGSFPHEFTAYRHVSDPVVRQSFEDAWGVELQSEPGL
                     RIPNMFEASLDGSFKGMYVQGEDFVQSDPNTHHVSAAMEALECCVVQDLFLNETAKYA
                     HVFLPGSSFLEKDGTFTNAERRVSRVRKV"
BASE COUNT          361 a          761 c          750 g          410 t
ORIGIN      
        1 accttgcgca cgcgcgaaac acggcgctcg gcgttggtga aggtcccatc cttttcgagg
       61 aacgaggagc cgggcaggaa gacgtgggcg tacttcgccg tctcattgag gaacaggtcc
      121 tgcaccacgc agcactccag cgcctccatg gcggcgctca cgtggtgggt gttgggatcg
      181 gactgcacga agtcctcgcc ctgcacgtac atacccttga agctaccgtc gagcgacgcc
      241 tcgaacatgt tcggaatgcg taaccctggc tcgctctgga gctcgacgcc ccaggcgtcc
      301 tcgaagctct ggcgcaccac cgggtccgag acgtgtcggt aggcggtgaa ctcgtgcggg
      361 aacgagccca tgtcgcacga gccctgcacg ttgttctgac cgcgcagcgg cgagacgccg
      421 acgccctcgc ggccgaggtt gccggtggcc atggcgagat tcgcgatgcc catgactgcg
      481 gtcgtgccct gactgtgttc ggtgactccg aggccgtaat aaatggcggc gttgcccgcc
      541 tgcgcataca tacgcgcggc gcggcgcacg cgctcggcgg gcaccccggt gatgctctcg
      601 gaggcctcag gcgagttacg cgcctcggcg acgaacgcct tccacttggc ccaactctct
      661 ggctcgcagc gctcggccac atacgcttcc ttggcgagcc cctcggtcac caccacgtgc
      721 gccagcgcat tgagcagcgc cacgttggtg ccgggcttga gctgcaggtg ctcatcggcc
      781 tcgacgtgcg gggagtgcac cagtcctatc tggcgcgggt cggcaatgat gagcttcgct
      841 ccctggcgca ggcggcgctt catctgcgag gcgaacaccg ggtggccgtc ggtcgggttg
      901 gcgccgatga ccatgatgac gtccgccttc atgaccgaat cgaaggcctg cgtgccggcc
      961 gactcaccga gtgtgttctt gagcccgtag ccggtgggtg aatgacacac gcgcgcgcag
     1021 gtatccacgt tgttgttgcc gaaggcggcg cgcaccagct tctgcaccag gaacgtctcc
     1081 tcgttggtgc agcgcgagga ggtgatgccg cccaccgagc cgcggccgta cttggcctgg
     1141 atgcgcttga actcgctcgc ggcgtagttg atcgcctcct cccacgagac ctcgcgccag
     1201 ttgtcgcgaa tggtcttgcg gatcatcggc tgcagctgcc ggtccgggtg agtcgcatag
     1261 ccccaggcga agcgcccctt cacgcaggca tggccgtggt tcgcaccgcc gtcgcgatcc
     1321 ggcaccatgc gcaccacctc ggcatcggct ccttcgccac gcatctccgc cgtgaagccg
     1381 cagccgacgc cgcagtaggc gcaggtggtt ttttcggccc gctccggcgc gccgagcccg
     1441 atgatcgact tctcggtcag cgctgcagtg ggacaggctt ccacacacgc gccgcaggaa
     1501 acgcactcgg actcgaggaa tggctggtcc tggctggccg ccaccttgga ggcaaagccg
     1561 cggccctgga tggtgagcgc aaaagtgccc tgcgtctcat cgcaggcccg cacgcagcgg
     1621 gaacaaacga tgcaaagatt cgggtcaaac gtgaaatagg gattgctctc atccttgggt
     1681 gcgccctgat gacgtttgcc gttggcgtac ggggttgatc tgaccccgag cttctccgcc
     1741 atggcgagca gctccgtgcg cgcctccgct tcggtcggct gacgctcgat cgggtagtcg
     1801 gaaagataca gctccatcac actgcgtcgc agctgcttga gcttcgggga ctcggtgcgc
     1861 actttcatgc ccgccgccac ggtcgtggtg caggaagccg ggtagccctt gcggccgtcg
     1921 atctcgcaca ggcacagccg gcacgagccg aaggccttga gggtgtcggt ggcgcagagc
     1981 ttcggcaccc tgacgccggc gagcgccgca gcgcgcatga tggaggtgcc gtcaggtacc
     2041 gtgaccgtgc ggccatcgat ctcgacggtg acgctgccgc tcgcatcggc cggcggcggg
     2101 gtaccgaaat cttcgtaatc tatcgcgtac atgtctcgcc tcgatgcgcg ctattgccgg
     2161 gtgcgcgcgc gaaagtcttc cctgaagtgc ttgagtgcgc tctgcacggg gaacggcgcc
     2221 atgccgccta ggccgcacag cgacccttgc accatggtct cacacagctc ctcgagcagt
     2281 tg
//
LOCUS       DATLKL010000181         2161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_2166, whole genome shotgun sequence.
ACCESSION   DATLKL010000181 DATLKL010000000
VERSION     DATLKL010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1274
                     /locus_tag="VM713_02355"
     CDS             120..1274
                     /locus_tag="VM713_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HVH83135.1"
                     /translation="MDRDQAIKLMQDLLRRVVEKKASDLFITAGFPPAIKIDGEIRPQ
                     SERALTAEQSAALVRAIMTDRQTKEFDATKECNFAIAPPGIGRFRVSAFVQQAAVGCI
                     IRLINAKIPTFEELDLPPVLKEVVLSKRGLVIVVGGTGSGKSTTLATMVGYRNEKTRG
                     HIVTIEDPVEYVHTHKGCVITHREVGVDTESWHAALKNTLRQAPDVILIGEIRDRETM
                     EYGIQFSETGHLVLATLHANSANQALDRVINFFPDERRDQLLMDLSLNIRALVSQRLV
                     PREAGTGRIAAMEIMLNSPLIQDLIFKGEVAKIKDVMSRSTRLGMKTFDQALFELYET
                     GFISYEDALRNADSKNELRLRIKLEGKREAKAVDDVAALQVVEEEHGRSL"
     gene            1274..>2161
                     /locus_tag="VM713_02360"
     CDS             1274..>2161
                     /locus_tag="VM713_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HVH83136.1"
                     /translation="MAAREGKAKSLDTTGTEATVSGGETLATVSASHPTLNTKPLFKL
                     MVEKKASDLFFTPNAPIKIKIEGQILPVNRQVLTPETVRQTAFALMSAEQREYFTREF
                     ELDFAVSEPGLGRFRVNVFMQRGYPAMVMRYITADMPRLDALGLPQVCADLVLLKRGL
                     ILMVGATGSGKSTTLAAMINHRNENSSDHIVTIEDPLEFLHTNKRSIVNQREVGLDTK
                     SYARALRGVVRAAPDVILIGEIRDRESMEAAINLAGTGHLVLATLHANNCAESLDRII
                     NMFPREQHNQIFLDLSHYLR"
BASE COUNT          420 a          732 c          681 g          328 t
ORIGIN      
        1 ctcgaccagt gcctcagcga gctcgtcaac aagggccacg tcagcaagga agaggcgcgt
       61 tacaaggcgc agaacaagga tgcgttgtag agggcggcgg gcttaaggag agctgatcca
      121 tggatcgcga ccaggccatc aagctgatgc aggacctgct gcggcgggtg gtggagaaga
      181 aggcctcgga cctgttcatc accgccggct ttccgccggc catcaagatc gacggcgaga
      241 tccgcccgca gtccgagcgg gcgctcaccg ccgagcagtc ggcggccctg gtgcgcgcga
      301 tcatgaccga ccgtcagacc aaggagttcg acgccaccaa ggagtgcaac ttcgcgatcg
      361 cgccgcccgg catcggacgc ttccgcgtca gcgccttcgt gcagcaggcc gccgtcggct
      421 gcatcatccg cctgatcaac gccaagatcc cgaccttcga ggagctcgac ctgccgccgg
      481 tcctcaagga ggtcgtgctg tcgaagcgcg ggctggtcat cgtcgtcggc gggaccggct
      541 cgggtaagtc gaccaccctc gccacgatgg tcggctatcg caacgagaag acccgcggcc
      601 acatcgtcac catcgaggat ccggtcgagt atgtgcacac ccacaagggc tgcgtcatca
      661 cgcaccgcga ggtgggcgtc gacaccgagt cctggcacgc ggcgctcaag aacaccctgc
      721 ggcaggcccc cgatgtgatc ctgatcggcg agatccgcga ccgcgagacc atggagtacg
      781 gcatccagtt ctccgagacc ggccacctgg tgcttgcgac gctgcacgcc aacagcgcca
      841 accaggcgct cgaccgcgtc atcaatttct ttcccgacga gcgacgcgat cagctgctca
      901 tggacctgtc gctcaatatc cgcgccctgg tgtcgcagcg gctggttccg cgcgaagccg
      961 ggacgggacg catcgccgcg atggagatca tgctcaactc gccgctgatc caggacctga
     1021 tcttcaaggg cgaagtcgcc aagatcaaag acgtgatgtc gcgctcgacg cggctcggca
     1081 tgaagacctt cgaccaggcg ctgttcgagc tctacgagac cggcttcatt tcctacgagg
     1141 atgcgctgcg caacgccgac tccaagaacg agctgcgctt gcgcatcaag ctcgaaggca
     1201 agcgcgaggc gaaggcggtc gacgacgtcg cggcgctgca ggtggtcgag gaagagcacg
     1261 gacgtagcct ctgatggcgg ccagggaagg gaaagcgaag tccctggaca ccacggggac
     1321 ggaggccact gtcagcggcg gcgaaacgct ggcgaccgtc agcgcgagcc acccgacgct
     1381 caacaccaag ccgctcttca agctcatggt ggagaagaag gcctccgacc tgttctttac
     1441 ccccaacgcg cccatcaaga tcaagatcga ggggcagatc ctgccggtga accgccaggt
     1501 gctgactccg gagacggtca ggcagacggc cttcgccctc atgtccgccg agcaacgcga
     1561 gtacttcacg cgcgagttcg agctcgactt cgcggtgtcc gagccggggc tcgggcgctt
     1621 ccgcgtcaac gtcttcatgc agcgcggcta cccggcgatg gtgatgcgct acatcaccgc
     1681 ggacatgccg cggctcgatg cgctcgggct gccccaggtg tgcgccgacc tggtgctgct
     1741 gaagcgcggg ctgatcctca tggtgggcgc caccggctcc ggcaagtcga ccacgctcgc
     1801 ggcgatgatc aatcaccgca acgagaactc ctccgaccac atcgtcacca tcgaggaccc
     1861 gctcgagttc ctgcacacca acaagcgctc gatcgtcaac cagcgcgagg tggggctcga
     1921 caccaagagc tacgcgcgcg cgctgcgcgg cgtggtgcgc gcagctcccg acgtgatcct
     1981 gatcggcgag atccgcgacc gcgagagcat ggaggcggcc atcaatctcg ccggcaccgg
     2041 ccacctggtg ctcgcgacgc tgcatgccaa taactgcgcc gagtcgctcg atcgcatcat
     2101 caatatgttc ccgcgcgagc agcacaacca gatcttcctc gacctgtcgc actacctgcg
     2161 c
//
LOCUS       DATLKL010000182         1688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_182757, whole genome shotgun sequence.
ACCESSION   DATLKL010000182 DATLKL010000000
VERSION     DATLKL010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182757"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /gene="ligD"
                     /locus_tag="VM713_02365"
     CDS             <1..1028
                     /gene="ligD"
                     /locus_tag="VM713_02365"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HVH83137.1"
                     /translation="RLDLEVGGARIRLTNLDRIYWPAAGKSHPAVTKRDLLRYLARVG
                     RFMLAHLQDRPLTMIRMPEGIEGERFFQKHWEQPLPEFVETVTVFSETKDDQHRYLLA
                     NNLPTLLWLGQIGTLEFHIWHSRARPGPDTPEASTDYAGSLAALESSVLNYPDYLVFD
                     IDPHIYSGKEAKGAEPELNRKGFAVGKRVAFWLRALLREMALEAIIKTSGKAGLHMFV
                     PIERTVTFDEARHICESVGRHVMRAHPKDITMDWAIEKRTGKIFIDYNMNVRGKTLNV
                     AYSPRGVPGTPVSMPVTWEELEAAEPMDFTLNNVPGRLEKRGDRWHDVLELKQSLTKA
                     FGVKGEK"
     gene            1040..>1688
                     /locus_tag="VM713_02370"
     CDS             1040..>1688
                     /locus_tag="VM713_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HVH83138.1"
                     /translation="MAASRSIGSLTVSFGLVAIPVKLFTANQSSNAISFNLLHKADGS
                     RLRQQYICQKDGAVVERDDMVKGYEFAKDQYVRFTPEEIKAMDEAGSHAVEISEFVPI
                     ESIDPVYYDKTYYLAPDKGAGKPYALLTEALKQAKRCGVGRWAARGKGYLVILRPIGD
                     VLAMQQLHYAADVRLASEVEIPKPEVKPAELKLAQQLIVAQTSRKFDHDVYKDEVR"
BASE COUNT          326 a          560 c          537 g          265 t
ORIGIN      
        1 cgcgactaga cctcgaggtc ggcggcgcgc gcatccgcct cactaatctc gaccgcatct
       61 actggcccgc ggcgggcaag agccatccgg cggtcaccaa gcgcgacctg ctgcgttacc
      121 tcgcgcgggt cgggcgcttc atgctggcgc accttcagga ccggcctctc accatgatcc
      181 gcatgcccga ggggatcgag ggcgagcgct tcttccagaa gcactgggag cagccgctcc
      241 cggagttcgt cgagaccgtc accgtgttct cggagaccaa ggacgaccag caccgctatc
      301 tcctcgccaa caacctgccg acgctcctgt ggctcgggca gatcggcacg ctcgagttcc
      361 acatctggca ttcccgcgcc cgtcccggcc ccgacacccc cgaggccagc accgactacg
      421 cgggctcact cgccgcgctc gagagctcgg tgctcaacta cccggactat ctggtcttcg
      481 acatcgatcc ccacatctac tcgggcaagg aggcgaaggg ggcggagccc gagctcaaca
      541 gaaagggctt cgcggtcgga aagcgcgtcg cgttctggct gcgcgcgctg ctcagggaaa
      601 tggcgctcga agccatcatc aagacctcgg gcaaggccgg ccttcacatg ttcgtgccga
      661 tcgagcgcac cgtgaccttc gacgaggccc gccacatctg cgagagcgtg ggccggcacg
      721 tcatgcgcgc gcatccgaag gacatcacca tggactgggc catcgagaag cgcaccggca
      781 agatcttcat cgactacaac atgaacgtgc ggggcaagac gctcaacgtc gcctactccc
      841 cccggggggt cccggggacg ccggtttcga tgcccgtcac ctgggaagag ctcgaggcgg
      901 ccgagccgat ggacttcact ctcaataatg tgccgggccg cttggaaaag aggggggacc
      961 ggtggcatga tgtactcgag ttaaaacaga gccttacgaa ggcttttggt gtcaagggtg
     1021 agaagtagag ggtgaagcta tggccgcgag ccgctcgata ggatctctca cggtgtcgtt
     1081 cggactggtc gcgatcccgg tgaagctctt caccgcgaac cagtcgtcga acgccatctc
     1141 cttcaacctg ctgcacaagg ccgacggctc gcgcctcagg cagcagtaca tctgccagaa
     1201 ggacggggcg gtggtcgagc gcgacgacat ggtcaagggc tacgagttcg ccaaggacca
     1261 gtacgtgcgc ttcacgcccg aggagatcaa ggccatggat gaggccggca gccacgcagt
     1321 cgagatctcc gagttcgtgc cgatcgagtc gatcgatccg gtgtattacg acaagaccta
     1381 ctacctggcc cccgacaagg gcgcgggcaa gccctatgcg ctcctcaccg aggcactcaa
     1441 gcaggccaag cgctgcggcg tcggccgctg ggccgcgcgc ggcaaagggt acctcgtgat
     1501 cctgcggccg atcggcgacg tgctcgccat gcagcagctg cattacgcgg ccgacgtgcg
     1561 cctcgcgagc gaggtggaga tccccaagcc cgaggtgaag ccggcggagc tcaagctcgc
     1621 ccagcagctc atcgtcgcgc agacctcccg gaagttcgac cacgatgtct ataaggacga
     1681 ggtgcggg
//
LOCUS       DATLKL010000183         1574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_81130, whole genome shotgun sequence.
ACCESSION   DATLKL010000183 DATLKL010000000
VERSION     DATLKL010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81130"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VM713_02375"
     CDS             <1..472
                     /locus_tag="VM713_02375"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HVH83139.1"
                     /translation="YAVEAFEAHAVGYLLKPVRKEQLAAALIRAGRLTRAQLQTLAAA
                     GEARRTHIAARSREGLKLIPIEEVRYFLADRKYTTVSHLNGEDLIEDSLRLLESEFGA
                     LFVRIHRNALVAVKHLERIERDADGQYFVRLRGSTAPLAVSRRMAGELKDRFRI"
     gene            complement(462..773)
                     /locus_tag="VM713_02380"
     CDS             complement(462..773)
                     /locus_tag="VM713_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH83140.1"
                     /translation="MSAQRLKVLIVDDEPPARERLRSLLGEIPEALVVGEAVSGAQAL
                     ALTHDVAPDVVLLDVRMPGMDGLEAARHLNVLEEPPAVIGRCWIRASVIILRFRAQLR
                     F"
     gene            complement(780..>1574)
                     /locus_tag="VM713_02385"
     CDS             complement(780..>1574)
                     /locus_tag="VM713_02385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVH83141.1"
                     /translation="VCAWLVGRAHVVVDTGGAALFPRDPVGFAVRNVWIALVVTGLAL
                     RYFYVAHEWRVSVELRAAARVHALQARIRPHFLFNSMNTIAALTRTDPARAESAVQDL
                     ADLFRATLSDKHNTITLAEELEVARTYQRMEQLRLGERLVVEWRTDSLPGAALVPGLM
                     IQPLLENAIYHGIEPRPDGGRVIISGEISAGLITLVVRNPLGAGPQQREGNRLALANI
                     RERLALVYGERALMKSGVFDGEYIVTLRFPLIERQSTQPSAPPAVA"
BASE COUNT          241 a          554 c          561 g          218 t
ORIGIN      
        1 gtacgcggtc gaggccttcg aggcacacgc cgtcgggtat ctgctcaagc cggtgcgcaa
       61 ggagcagctc gccgccgcgc tgatccgcgc cggacgcctg acccgcgcgc agctgcagac
      121 gctcgcggcc gccggcgagg cacgccgcac ccacatcgcg gcgcgctcgc gcgaggggct
      181 gaagctcatt ccgatcgagg aggtgcgcta cttccttgcc gaccgcaagt acaccacggt
      241 gtcccacctg aacggcgagg atctcatcga ggactcgctg cggctgctcg agagcgagtt
      301 cggcgcgctc ttcgtgcgca tccaccgcaa cgcgctggtc gcggtaaagc acctcgagcg
      361 catcgagcgc gacgccgacg gccagtactt cgtgcgcctg cgcggctcaa cggcgccgct
      421 cgcggtgagc cggcgcatgg cgggcgagct caaggaccgg ttcagaatct gagctgcgct
      481 cggaagcgga ggatgatcac tgaggcgcgg atccaacacc ggccgatcac cgccggcggc
      541 tcctccagca cgttgaggtg tcgcgccgcc tcgagcccgt ccatgccggg catgcgcacg
      601 tcgagcagca cgacgtcggg ggcgacatcg tgcgtgagcg ccagtgcctg cgcgccactc
      661 accgcttcac ccaccaccag tgcctcgggg atctcgccga ggaggctgcg cagccgctcg
      721 cgcgccggcg gctcgtcgtc gacgatcaac accttgaggc gctgcgcact cacgggactt
      781 caggcgaccg caggcggcgc ggacggctgc gtcgattgcc gctcgatgag cggaaagcgc
      841 agcgtgacga tgtactctcc gtcgaagacc ccggatttca tgagcgcgcg ctcgccgtac
      901 acgagggcga gccgctcgcg gatgttggcg agcgccaggc ggttgccctc gcgctgctgc
      961 ggcccggcgc cgagcggatt gcggaccacg agggtgatga gcccggcgct gatctcgccg
     1021 ctgatgatca cccggccgcc gtccgggcgc ggctcgatgc cgtggtagat ggcgttctcg
     1081 agcagcggct ggatcatgag gcccggcacc agcgccgcgc ccgggagcga gtcggtcctc
     1141 cactcgacca ccagccgctc gcccaggcgc agctgctcca tgcgctggta ggtgcgcgcc
     1201 acctcgagct cctcggccag ggtgatggtg ttgtgcttgt cgctcaaagt ggcacggaag
     1261 agatcggcga ggtcctgcac cgcgctctcg gcgcgggccg ggtcggtgcg ggtgagggcg
     1321 gcgatggtgt tcatgctgtt gaagagaaag tgcggacgga tgcgcgcctg cagcgcgtgc
     1381 acgcgggcgg cggcccgcag ctcgacgctg acgcgccact cgtgcgccac gtagaagtag
     1441 cgcagcgcca gcccggtcac caccagggcg atccagacgt tgcgtaccgc gaagcccacc
     1501 ggatcccggg gaaagagcgc cgcgccgccc gtgtcgacca ccacgtgggc gcggccgacg
     1561 agccaggcgc acac
//
LOCUS       DATLKL010000184         4396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_204989, whole genome shotgun sequence.
ACCESSION   DATLKL010000184 DATLKL010000000
VERSION     DATLKL010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204989"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VM713_02390"
     CDS             complement(<1..473)
                     /locus_tag="VM713_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83142.1"
                     /translation="MSRCLAPLLLLASSAHAESVAITGADVWTNTSDAPVRNATIVIS
                     GGRIVSIQSAGPPPPSTRIFEAKGRIVTPPLDAAATQIGLVEVASASDTDDRAVHGGP
                     LGAAFDVSFGVDANGLTVQQARAAGVARALVFPNPAGNGFFAGQAARLNLAERTD"
     gene            complement(470..1873)
                     /locus_tag="VM713_02395"
     CDS             complement(470..1873)
                     /locus_tag="VM713_02395"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVH83143.1"
                     /translation="MNIAHRAILVALSAAACLHGAHAAAPVGVAPLRQAALEPPQSHY
                     RPLPRVDTLLTGATILDGVGGRIEHGDVLMVDGRIAAVGRALSHQGAIVVDASGRWIT
                     PGIIDVHTHYGTYVLPQSAAEANASDVLEESDPNVADTWIEHAVRPGDPAFSRALASG
                     VTTLQILPGSGALFGGRTVIVKPVPAVTIDQMRFPGAPQGLKMACGSNPAVSFGGRGR
                     FPNSRQGEIAGVRSAFLDAQRYMKEWDDYRAGRRTEAPRRDLKLDTLSAALRGELRVH
                     VHCYRADDISNWISTLEEFGVHRVTVHHAAEGYKIAPLLAQKQVCAAVWPDWWGFKRE
                     AEDGVPENAAFIDAAGGCVVMHSDIPVLGSLLNIEAAKAAAAGRRAGLAIPEEHAIRW
                     ITSNAARVLGLESQIGHLAPGMNADVVVWSADPFSIYSQADLVFIDGAVAYDRHGPAA
                     QRSSDVELGRPQRERVR"
     gene            complement(1881..2465)
                     /locus_tag="VM713_02400"
     CDS             complement(1881..2465)
                     /locus_tag="VM713_02400"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HVH83144.1"
                     /translation="MDARFAPRSHEDILRIVGDYPLAWVISHDFQATPLPLLAETDAS
                     GSITSLLGHFARRNPQVAALQADPAALILIQGPQGYISPRLVSNRTWAPTWNYAVVRF
                     EVTVAFVTEETDAAVRRLAAHLEDGRRKPWRVEELGKRYGEPLPRIIAFRARVHAVRA
                     TFKLGQDEARATFNEIVAGLGDTPLARLMSEQDK"
     gene            complement(2469..3431)
                     /locus_tag="VM713_02405"
     CDS             complement(2469..3431)
                     /locus_tag="VM713_02405"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HVH83145.1"
                     /translation="MSDAGGIDPEDGLDPDIRRFVTAVQRSYGQYPKFEALPPAERRR
                     IAEDVRAPWRVGGPAMWRTADTVIAGVRARIHIPVEASARGAMLYLHGGGWTMFSIDT
                     HDRLMREYAERSGSVVVGLDYSLSPEAKFPTALNEVVAAIGWLRRQGTTYGIDPLRLA
                     IGGDSAGGNLAVAAALTLREQGEPPLRAMLLNYGAFSPEPTASFERYGGPDYMLTVEE
                     MQSFWSNYTSDPAELNNPLVAPYRADLRGLSPALLCIAGCDILADSNRAMAARFAAAQ
                     VPCEVHLYAGATHSFLEAVSVAPLAERAIAEAAGWLREQLARAR"
     gene            3627..>4396
                     /locus_tag="VM713_02410"
     CDS             3627..>4396
                     /locus_tag="VM713_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVH83146.1"
                     /translation="MKHSADLLGVARPVIALNDEYPPGFVDPMHSHDRTQILYASSGV
                     MSVRTDQSTSVVPPQRAVWIPAGMMHEVSCRGAVSLRTLYIEATLGCDPKRCRVFEVS
                     PFLRALILEVVGFNPNYDIDGREGRIVGLMLEELDRMPTAPYFVNMPGDPRLLRVCRA
                     IIANPADSRDLDELAKAAGMGRRTFTRAFKNETGMGVAIWRQQARLVHALSLLSAGQP
                     ITQVAFEVGYDSASAFTAMFHRSFGVPPSQFEVRPAARA"
BASE COUNT          748 a         1487 c         1441 g          720 t
ORIGIN      
        1 atatcggtgc gctcggcgag gttaagccgt gccgcctggc ctgcgaaaaa gccgttgccg
       61 gcaggattcg gaaagacgag cgcgcgcgcg acacccgcgg cgcgcgcctg ctggacggtc
      121 aggccgttcg catcgacacc gaacgataca tcgaatgccg cgccgagcgg tccgccgtga
      181 acggcgcgat cgtcggtgtc gctcgcgctt gccacctcga ccaggccgat ctgcgtggcc
      241 gctgcatcga gcggaggcgt cacgatccgg cccttcgcct cgaaaatcct cgtggaaggg
      301 ggcggtggtc cagcggactg gatcgacacg atccgcccgc cggagatcac gattgtggca
      361 ttcctcaccg gggcgtcgga cgtgttcgtc cagacgtcgg caccggtgat ggcaaccgac
      421 tcggcgtgtg cgctcgaggc gaggagcagc agcggcgcca ggcagcggct caccgcacgc
      481 gttccctctg cggcctgccg agctcgacat cgctggaacg ctgcgcggca gggccgtggc
      541 gatcgtaggc gaccgctccg tcgatgaaca ccaggtccgc ctggctgtag atgctgaagg
      601 ggtcggccga ccatacgacc acatcggcat tcattcccgg tgccagatgt ccgatctgcg
      661 attcgagccc cagcaccctc gcggcattgg aagtgatcca gcggatcgcg tgctcctcgg
      721 gaatcgcgag cccggcgcgg cgacccgcgg ccgccgcctt ggccgcttcg atattgagga
      781 ggctcccgag caccggaata tcggagtgca tcacgacgca accaccggcg gcgtcgatga
      841 aagccgcgtt ctcaggaacg ccgtcttccg cttcgcgctt gaatccccac caatccggcc
      901 agacggccgc gcacacctgc ttctgtgcca aaagcggcgc gatcttgtag ccctccgccg
      961 catgatgcac cgtcacgcgg tgcacgccga attcctcgag cgtcgagatc cagttcgaga
     1021 tgtcgtcggc tcggtagcaa tgcacgtgaa cccgcagctc gccgcgcagc gccgcggaca
     1081 gggtgtcgag cttcagatca cgccgcggtg cctccgttcg ccgtccagcg cgatagtcgt
     1141 cccactcctt catatagcgc tgcgcatcga ggaaggcgct gcgcacgccg gcgatctccc
     1201 cctggcggct attcgggaag cggccacgac cgccaaagct cacggctggg ttgctgccgc
     1261 aggccatctt gagtccttgc ggcgcaccgg ggaaacgcat ctgatcaatg gtgaccgccg
     1321 gcaccggctt gacgatgacg gtgcgaccgc cgaagagcgc gcctgagccg ggcaggatct
     1381 gcagcgtcgt gacgccactc gcgagggcgc gggagaacgc cgggtcgccg ggacgtacgg
     1441 cgtgctcgat ccaggtatcg gctacgttcg gatcgctctc ctcgaggacg tccgaggcgt
     1501 tcgcttccgc ggcgctttgc ggcagcacgt atgtcccata gtgtgtatga acgtcgatga
     1561 tgccgggggt gatccagcga ccactggcat cgacgacgat tgcgccttgg tgcgagagcg
     1621 cacggccgac cgctgcgatc cgcccatcaa ccatcaagac gtcgccgtgc tcgatccgcc
     1681 cgcccacccc gtcgaggatc gtcgctcccg tgagcagagt gtcgacgcgc ggcagggggc
     1741 gatagtggct ctgcggcggc tcgagcgctg cctggcgcag cggcgcgacg cccaccggcg
     1801 cagcggcgtg cgcgccgtgc agacacgcag cggcgctcag ggcgacaagg attgcgcggt
     1861 gcgcgatatt catcgctcgc tcacttgtcc tgctccgaca tcagccgcgc gagcggcgta
     1921 tcaccgaggc cggcgacaat ctcgttgaac gtggcgcgcg cttcgtcctg accgagcttg
     1981 aacgtggcac gcaccgcgtg cacgcgcgca cgaaatgcga tgattcgcgg cagcggctcg
     2041 ccataccgct tgcccagttc ctccacccgc cagggtttgc gccgcccgtc ctccaggtga
     2101 gcggcgaggc gccgaacggc cgcatccgtt tcctccgtca caaacgcgac cgtgacttcg
     2161 aaacgcacga ccgcgtagtt ccacgtcggg gcccaggtcc ggttcgagac cagccgcggg
     2221 gaaatatagc cctgcggacc ttgaataagg atcagggcag cagggtcggc ctgcaaggcc
     2281 gccacctgcg ggttacggcg ggcgaagtgt cccagcaggc tcgtaatgga accggatgcg
     2341 tcagtctctg cgaggagcgg cagcggtgtc gcttgaaagt cgtgcgagat gacccaggcg
     2401 agcgggtagt ccccgacgat cctgagaatg tcctcgtgcg agcggggcgc gaagcgcgca
     2461 tccatgactc acctagccct ggcgagctgc tcgcgcagcc agccggcggc ctcggcgatc
     2521 gcgcgctccg cgagcggggc gaccgacacc gcctcgagaa agctgtgcgt agcaccggca
     2581 tagaggtgca cctcgcacgg cacctgtgcg gcggcgaagc gtgcggccat ggcacggttg
     2641 ctgtcggcaa ggatgtcgca gcccgcgatg cagagcaggg cgggagacaa gccgcgcaag
     2701 tccgcgcgat acggagccac caggggattg ttgagctcgg ccgggtcgga ggtgtaattg
     2761 ctccagaagc tctgcatctc ctcgaccgtg agcatgtagt ccggccctcc atagcgctcg
     2821 aaactggcgg tcggctccgg gctgaatgcg ccgtagttga gcagcatcgc tcgcagcggc
     2881 ggctccccct gctcgcgcag agtcaatgcg gctgcgaccg caaggttgcc acccgcggaa
     2941 tccccaccga tggcgagccg cagcggatcg atgccgtacg ttgtcccctg ccggcggagc
     3001 catccgatgg ctgcaaccac ctcattgagc gccgtgggga acttggcctc cggcgacaga
     3061 ctgtagtcga ggcccacgac gaccgagccc gatcgctcgg catactcgcg catcagccga
     3121 tcatgcgtgt cgatgctgaa catcgtccac ccgccaccgt gaagatagag catcgcgccg
     3181 cgcgcactcg cctcgaccgg tatgtggatg cgcgctcgga ctcccgcgat cacggtgtcc
     3241 gcggtgcgcc acatcgcagg tccaccaacc cgccacgggg ctcgcacgtc ttcggcaatt
     3301 ctgcgacgct ctgcgggagg caatgcctcg aatttggggt actgcccata gcttcgctgc
     3361 accgcagtca cgaagcgacg aatgtcggga tcgagcccgt cttccggatc gatcccccct
     3421 gcatcgctca tccggggccc cttctgcctg cgcaacgaga gccgagattg acggggatcc
     3481 tagccgaccg ggcgcgcggg ccctgcgtgg atagggtcaa tcactgccga gactgggcca
     3541 tcgctcaaag ggctccgccg cgacttgcag acccaggcag tttggccata ctgcgacaaa
     3601 aggcagcata gcgtcgagtt aaggccatga agcacagcgc cgatctgctc ggagtcgctc
     3661 ggcccgtcat cgcgctgaac gacgagtatc cacccgggtt cgtcgacccg atgcattcgc
     3721 acgatcgcac gcagatcctg tacgcctcat cgggcgtcat gtcagtgcgc accgatcaat
     3781 ctacctccgt cgtcccgcca cagcgcgccg tgtggatccc ggcaggaatg atgcacgagg
     3841 tgtcctgtcg cggtgccgtg tcactcagaa ctctctatat agaggcgacc ctgggttgtg
     3901 accccaagcg ctgccgggtc ttcgaagtgt ctcccttcct gcgggcgttg attcttgagg
     3961 tggtgggatt caatccgaac tacgacatcg acggccggga aggccggatc gtggggctca
     4021 tgctcgaaga gctggaccgc atgccgaccg ccccgtattt cgtaaatatg cccggcgatc
     4081 cgaggctgct gcgtgtctgt cgcgccatca tcgcgaaccc ggcagacagc cgcgatctcg
     4141 acgagctggc caaggccgcc gggatgggac gccgaacatt cacgcgcgcg ttcaaaaacg
     4201 agacgggcat gggagtggcg atctggcggc agcaggcgcg cctcgtccac gcgctatcgc
     4261 tgctgtccgc cggccagccg atcacccagg tggcgttcga agtcggttat gacagcgcca
     4321 gtgctttcac cgcgatgttc catcggtcgt ttggcgtgcc ccccagccag ttcgaggtgc
     4381 gccccgccgc tcgagc
//
LOCUS       DATLKL010000185         2414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_81150, whole genome shotgun sequence.
ACCESSION   DATLKL010000185 DATLKL010000000
VERSION     DATLKL010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81150"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..447)
                     /gene="rpsL"
                     /locus_tag="VM713_02415"
     CDS             complement(64..447)
                     /gene="rpsL"
                     /locus_tag="VM713_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HVH83147.1"
                     /translation="MATTGQLIRQPRRLGAEKTKVPALGASPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGIEVTTYIPGVGHNLQEHSIVLIRGGRVKDLPGVRYHVVRGTL
                     DTAGVTGRQKSRSKYGAKRPKGTAK"
     gene            889..>2414
                     /locus_tag="VM713_02420"
     CDS             889..>2414
                     /locus_tag="VM713_02420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVH83148.1"
                     /translation="MEVLDLTTSAPTVAEVTAGSLDSRFRPFTARGSHSIRTVLWLRL
                     PLPEAPAGRGTPVLLARSGLDQPVELFARRNGAVVALKAVAVAPKFGGGQDTVFALLP
                     APDSGAPLYARISRTGDTTTDVQFAASTLERTLERAAAHSRMISFAFGALMAMALSGL
                     LVRLVLTDALYPLYGTLFGLEAIYLAYCSGEGFSWPLLFYARPLGWYAWNVPIAVAGS
                     AASLFVREFANLRLFSARVYRAFGWLAVGFLVLAVSNVLRLVGLAALVAAVGNLLFLG
                     SAVFTLLVSYLAWRRGNRAAGWFLIAWALLCTFQIATAAWLLNGRADHADALLCYGLA
                     PSMVAAAMLIALGVADRMRAQTVALTEAERRTQTDALTGVLNRSTLIERLDAACLGPQ
                     RRGLPVSVLFIDLDHFKRINDTRGHAAGDARLAAVVASIQAELRQSDLIGRYGGEEFV
                     VVLTGADEAAAAPVAQRICQRVADVRVEGFSAPIRLTCSIGVAGSDTLGVWGQHLVAQ
                     A"
BASE COUNT          292 a          860 c          798 g          464 t
ORIGIN      
        1 aggtctgcgt ctgcgtggca tctattcctc aatcgcgcac aacgctgcgc tgtgcgctcc
       61 ttcttacttt gccgtccctt tcggccgttt cgctccgtat ttcgaccggc tcttctgccg
      121 gccggtgacg cccgccgtat ccagcgtgcc gcggactacg tggtaccgta cgcccggaag
      181 gtccttcacg cgacctccgc ggatcaagac gatcgaatgc tcctgaagat tgtggcccac
      241 gccgggaata taagtagtca cttcgatccc gttggtgagc cgtacgcgcg ctactttgcg
      301 cagcgccgaa ttgggcttct tgggcgtcgt ggtgtagacg cgcgtgcaca ccccgcgctt
      361 ctggggtgag gccccgagcg ccggtacttt ggttttttcc gcccccagcc ggcgcggttg
      421 ccggatgagc tgacccgtgg tcgccatcgt tacactcgtc tccaagctca ggtgcggggg
      481 tgggtactcg cacaatgggc gacccagaac aaccgggccg cctgtgtcag gcggcccggt
      541 gcactgaagt cgctgtttcc ggtgccggcg tgctaatgaa gggccggccc gacaaaggcc
      601 ggcgattcta ggtaggaacc ccgggagtgt caaccggcca tccggtgagg gctgctgctt
      661 tgcgattgat ttggcgcctc gccccgttac gatagctgca aacgggcccg gagcgctccc
      721 gcgccccact gttcagacta ataaggtatc gacccggaca gggtcaggga ctccgtgctc
      781 tcgagggacc tcaggcggct tttgggtctg gcggcgtgtc tggcgggagg cctcgcatca
      841 gccgggaccg ccccggcagc cccgcctgcc ctcgagaccc cccttccgat ggaggttctt
      901 gacctgacca cctcggcgcc tacggtggct gaggtaaccg ctgggtcgct cgatagccgc
      961 ttccgcccct tcaccgcgcg cggctcccat tccatccgga ccgtgctctg gctccggctg
     1021 ccactccccg aggccccggc gggccgcggg actccggtgc tgctcgcccg ctccgggctg
     1081 gatcagccgg tcgagttgtt cgcgcggcgc aatggggcgg tcgtggccct caaggccgtc
     1141 gccgtggcgc ccaagttcgg gggcggacag gacacggtct tcgcgctcct tcctgccccg
     1201 gattccggcg ccccgcttta cgcgcggatc tcccgcacgg gcgataccac gacagatgtg
     1261 cagttcgccg cctcgacgct cgagcgcacg ctcgagcgcg ccgccgccca ctcccgcatg
     1321 atctccttcg ccttcggcgc cctcatggcg atggcgcttt cggggctgct cgtgcgcctc
     1381 gtgctgaccg atgcgcttta cccgctctac gggacgctgt tcgggctcga ggccatctat
     1441 ctcgcgtact gctcgggcga ggggttcagc tggccactcc ttttctatgc gcggccgctg
     1501 ggctggtacg cctggaacgt gccgattgcg gtcgcgggca gcgccgcctc tctgttcgtg
     1561 cgcgagttcg ccaacctgcg gctgttctcc gcgcgcgtct accgcgcctt cggctggctc
     1621 gcggtcggct ttctggtgct cgccgtctcg aacgtgctgc gtctcgtcgg gctcgcggcg
     1681 ctggtcgccg ccgtgggcaa tctcctgttt ctcgggagcg cggtcttcac gctcctcgtg
     1741 tcctacctcg cctggcggcg cggcaaccgc gccgccggct ggttcctcat cgcctgggcg
     1801 ctgctctgca cgttccagat cgcgaccgcg gcgtggctcc tcaacggtcg ggccgaccac
     1861 gccgacgcac ttctctgcta cggactcgca ccctcgatgg tggctgccgc catgctgatc
     1921 gcgctcgggg tagccgaccg catgcgggcc cagaccgtcg ctctcaccga ggccgagcgg
     1981 cgcacgcaga ccgacgccct caccggcgtg ctcaaccgct cgacactcat cgagcggctg
     2041 gacgcggcgt gcctgggtcc gcagcggcgc gggctcccgg tctcggtcct gttcatcgat
     2101 ctcgatcact tcaagcggat caacgatacc cgcgggcatg cggcgggcga tgcccgcctc
     2161 gccgccgtgg tcgcgtcgat ccaggcggag ctgcgccagt cggacctcat cggacgctat
     2221 gggggcgagg agttcgtggt ggtgctcacc ggggcggatg aagcggccgc agccccggtg
     2281 gcgcagcgca tctgccagcg cgtcgcggac gtgcgggtcg aaggcttctc cgctccgatc
     2341 cgcctcacct gcagcatcgg tgtggcgggc agcgataccc tcggggtgtg ggggcagcac
     2401 ctggtcgcgc aggc
//
LOCUS       DATLKL010000186         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_160082, whole genome shotgun sequence.
ACCESSION   DATLKL010000186 DATLKL010000000
VERSION     DATLKL010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160082"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VM713_02425"
     CDS             complement(<1..884)
                     /locus_tag="VM713_02425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVH83149.1"
                     /translation="MAPLLFFDPLCPRPYGEESLRAGGLAGTEASTVRVAEALDAWVA
                     QHNRTEAQGRYRPPSAVAHLRHVVVLRDPRALADAARLFPEARLTLWVHDRIEPGSSR
                     SRWFARAAPELRRLQPDVVCVSDYQRARVAATLAGLPGCERLVVRRIYNPVDDAIVPD
                     GTPVDRAKLVFFSSPNKGLRFALDAFRALRRRMPDLRLCVGNPGYKSFAAAATPGVEW
                     LGSLPHARVLAEARAALCTFSPNFVIPETFGLVFAESHAVGTPVLTYDCGAAREVLGG
                     EQELLPVTWAQRAYEAPLR"
     gene            966..2030
                     /locus_tag="VM713_02430"
     CDS             966..2030
                     /locus_tag="VM713_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83150.1"
                     /translation="MSTPSARLLFIPVSGPRGMGEYARCLMIATALARRVPGVEMHFA
                     LSREAPYAASTPFPTTLLPSSATFHPREVRALIQDLKPRVVVFDNAGRTVQLRAAHAA
                     GARVVYVSSRRRQRRKGFRLQWMRLIDEHWIAYPRFIAGAVTPLERLKLRALKRPAVR
                     FIDTLLLAADLERSNECLARFALQAGGYALIVPGGGSAHRGAENAPAIVASAAHAIAR
                     RGLPTLLVGSDDAGAGGERPALLRVAPRLPPAELIELIRTARLVVCNGGDTLLQVLAC
                     ELPCVAVPIAHDQPARIDRCVRAGLAVRSELNARAIESTAFAVLEQGRRPAVGEPSPG
                     HPRVRNRMEEVLSTLTALVG"
BASE COUNT          315 a          780 c          779 g          341 t
ORIGIN      
        1 cggagcggtg cctcgtaggc gcgctgtgcc caggtcaccg gcagcagctc ttgctcgccg
       61 ccgagcacct cgcgtgcggc tccgcagtcg taggtcagca ccggcgtgcc cacggcgtgc
      121 gactcggcga acaccaggcc gaaggtctcg gggatcacga agttggggct gaaggtgcag
      181 agcgcggcgc gcgcctcggc gagtacgcgc gcgtgcggca gcgacccgag ccattccacc
      241 ccgggggtgg cggccgcggc gaagctcttg tacccgggat tgccgacaca cagccgcagg
      301 tccggcatgc gccgccgcag ggcgcgaaac gcatcgagcg cgaagcgcag tcccttgttg
      361 ggggaggaga agaacaccag tttcgcccga tcgacggggg tgccgtcggg cacgatggcg
      421 tcatcgaccg gattgtagat gcgtcgcacg acgagtcgct cgcagccagg aagccccgcc
      481 agcgtcgccg ccacccgcgc gcgctggtag tcagacacgc acacgacgtc gggctgcagc
      541 cggcgcagct ccggggcggc gcgcgcgaac cagcgcgagc gcgatgagcc cggctcgata
      601 cgatcgtgca cccagagcgt cagacgcgcc tcgggaaaga gccgcgcggc atccgcgagc
      661 gcccgcgggt cgcgcagcac cacgacgtgc cgcaggtgcg ccacggcgct cggcggccgg
      721 tagcgcccct gcgcctcggt gcggttgtgc tgcgccaccc aggcatcgag cgcctccgcg
      781 acgcgcaccg tgctcgcttc ggtgcctgcc agcccccccg cgcgcaggct ctcctcgcca
      841 taggggcgcg ggcacagcgg atcgaagaac aggagcggtg ccatcgtgcg gctcgatctt
      901 tcagcggcgc gcggcatcac gctgcgggag acttgccgca gcgcagtact ctaccattcg
      961 cacccgtgtc gaccccgagc gcgcggttgt tgttcattcc ggtatccggc ccgcggggta
     1021 tgggagagta cgcgcgctgc ctcatgatcg ccacggctct cgcgcgccgc gtgccgggtg
     1081 tggagatgca cttcgctttg agccgggaag cgccctacgc cgccagcacg ccctttccca
     1141 ccaccctgct gccctcctct gcgactttcc atccgcgtga ggtgcgcgcc ctgatccaag
     1201 acctcaagcc gcgggtcgtg gtgttcgata acgccgggcg gaccgtgcag ctgcgcgcgg
     1261 cgcacgcagc cggtgcgcgc gtcgtctatg tgagctcgcg gcgccgccag cgccgcaagg
     1321 gattccggct gcagtggatg cgcctgatcg acgagcactg gatcgcctac ccgcgcttca
     1381 ttgccggcgc ggtcactccg ctcgagcgtc tgaaattgcg ggcgctcaag cgcccggccg
     1441 tccgcttcat cgacacgctg ctgctcgccg cggacctgga gcgctcgaac gagtgcctgg
     1501 cgcgcttcgc gcttcaggcc ggcggctacg cgctgatcgt tcccggcggc ggcagcgcgc
     1561 accggggcgc cgagaacgcg ccggccatcg tcgcctccgc cgcgcacgcc atcgcgaggc
     1621 gtggcctacc gacgctgctg gtgggctcag acgatgccgg cgcaggcggc gaacgtccgg
     1681 cccttctcag agtggcaccg cggctgccgc cggcggagct tatcgagctg atccgtaccg
     1741 cgcgcctcgt ggtctgtaac ggcggcgaca cgctgctgca ggtgctcgcc tgtgagctgc
     1801 cgtgtgtcgc cgtcccgatc gcgcacgatc agcccgcgcg catcgaccgt tgcgtgcgag
     1861 cggggctcgc ggtgcgctcc gagctcaacg cgcgcgccat cgagagcacg gcgttcgcgg
     1921 tcctcgagca aggccggcgg cccgcggttg gcgagccctc cccgggtcat ccgcgggtgc
     1981 gcaatcgcat ggaggaagtg ctgagcacct tgacggcgct cgttggttga ttggcgcgca
     2041 ggtcgatcgc cggtgggcgt gtggtgatcc ccgaaaatgc tttccgggag caataggtcg
     2101 aaagtcatct atgtatgctc tccgggaccc ctgtaggggg cctcaaggag ccatagttag
     2161 acgcttttct gatatggaac gaccggtcaa ggggttgatt ccgagtcctc attct
//
LOCUS       DATLKL010000187         1925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_205023, whole genome shotgun sequence.
ACCESSION   DATLKL010000187 DATLKL010000000
VERSION     DATLKL010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205023"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VM713_02435"
     CDS             complement(<1..507)
                     /locus_tag="VM713_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83151.1"
                     /translation="MRLATETGAHHVRPLVIWNKYALTLAAFVLALLAVPAGAQPVCA
                     AGQAGQSASARLERAMAEGRFIAYEPTSLQVVDGRTHDAEPASIRADLEVLRPRFDAL
                     ITYDAVHGAQAIPAIAAALKFRALVVGVWDPADPTQLNAALKAARDYPQLVVGLSLGN
                     EAIFARRSS"
     gene            520..1344
                     /locus_tag="VM713_02440"
     CDS             520..1344
                     /locus_tag="VM713_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HVH83152.1"
                     /translation="MNTAQADKAGRFRALHQRAGTFVIPNVWDGASACVMAGLEFEAL
                     ATSSSACAGTLGRLDGQITRDEALAHARVIVAACALPVSADLEQGFGDSPETVAETIR
                     QAAAAGLVGGSIEDASGNPRAPLYDFELAVERVAAAVEAARQLPFPFTLTARAENYLH
                     GRPDLDDTLRRLEAFERAGADVLFAPGLPDLATVRTVCSAVHKPVNFMVGIRGRSFSV
                     AELAAAGVKRISLSTSLYRAAMSGLYAAGREVKDSGTFSYVESIMSGKDLAGFIER"
     gene            complement(1331..1819)
                     /locus_tag="VM713_02445"
     CDS             complement(1331..1819)
                     /locus_tag="VM713_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HVH83153.1"
                     /translation="MKLISTSLLDELAAAAAASPRGRTHYNLHGSAADPVQRYLVVAN
                     RGSYFRPHRHTTRAELALVVRGELDLLVFDDARRISARYAFGAGAGNIAYENGPGIWH
                     TVVVQADATTFLEVKQGPYDPATASEFAAWAPAEDDRDVARFLEWARHAAPGDTAPAS
                     AR"
BASE COUNT          288 a          674 c          679 g          284 t
ORIGIN      
        1 gctcgagcgg cgcgcgaaga tcgcctcgtt gccgagcgac agcccgacca cgagctgcgg
       61 ataatcccgc gcggctttga gcgccgcgtt gagctgcgtg gggtccgccg gatcccacac
      121 gccgacgacg agcgcacgaa atttgagggc cgcggcaatg gccgggatcg cctgggcgcc
      181 gtggaccgca tcgtaggtga tcagcgcatc gaagcgcggg cgcagcacct cgaggtcggc
      241 acggatgctc gccggctcgg cgtcgtgggt gcgtccgtcc actacctgaa gcgaggtcgg
      301 ctcgtaggcg atgaagcgcc cctcggccat cgcgcgctcc aggcgcgcac tggccgactg
      361 ccccgcctga ccggccgcgc acaccggttg cgcgccggcg ggcaccgcga gcagcgccag
      421 cacgaacgcc gcgagcgtca gggcgtactt gttccagatc accaggggcc taacatggtg
      481 cgcgccggtc tccgtggcca gcctcaaggg gagtattcca tgaacactgc gcaagcggac
      541 aaggcggggc gcttccgcgc gctccaccag cgcgccggaa ccttcgtcat tcccaacgtg
      601 tgggacggcg cttcggcgtg cgtcatggcc ggactcgagt tcgaggcgct cgccacctcg
      661 agctccgcgt gcgcgggcac gctcggcagg ctcgacgggc agatcacgcg cgatgaagcg
      721 ctcgcgcacg cgcgcgtcat cgtcgccgcc tgcgctctgc cggtctcggc cgacctcgag
      781 cagggattcg gcgactcccc ggagacggtg gcggagacga tccgccaggc ggcggcggcg
      841 gggctcgtgg gcgggtcgat cgaggatgcc agcggcaatc cgcgcgcgcc gctctacgac
      901 ttcgagctcg cggtcgagcg cgtcgctgcg gcggtcgagg ctgcgcgcca gcttcccttt
      961 ccgttcacac tcacggcgcg tgccgagaac tatctccacg gccgtcccga cctcgacgat
     1021 acgctgcggc ggctcgaggc tttcgagcgc gccggtgccg acgtgctgtt tgctcccggg
     1081 ttgcccgacc tcgccaccgt gcgcacggtt tgcagcgcag tgcacaagcc cgtcaacttc
     1141 atggtcggca tccgtggccg ctccttcagc gtggcggagc tcgctgccgc cggcgtcaag
     1201 cgcatcagcc tgtcgacctc gctgtatcgc gccgcgatga gcggactgta cgccgcaggg
     1261 cgcgaagtga aggactccgg gaccttcagc tacgtcgagt cgatcatgag cggcaaggat
     1321 ctggcgggct ttatcgagcg ctagccggcg ccgtgtcacc cggcgcggcg tgcctcgccc
     1381 attcgaggaa gcgcgcgaca tcccggtcgt cctctgccgg cgcccaggcg gcgaactccg
     1441 aggcggtcgc aggatcgtaa ggcccctgtt tgacctcgag aaatgtcgtg gcgtcggcct
     1501 ggacgaccac cgtgtgccag atcccaggac cattctcgta ggcgatgttg cccgcaccgg
     1561 cgccgaaggc atagcgcgcc gagatccggc gcgcatcatc gaacacgagc agatccagct
     1621 cgccgcgcac gacgagtgcc agctcggcgc gggtcgtatg gcggtgcggg cggaagtagg
     1681 agccgcggtt cgcgaccacc agatagcgct gcaccggatc ggcggccgag ccatgcaggt
     1741 tgtaatgggt gcgcccgcgc gggctcgccg ccgcggccgc cgccagctcg tccaacaggc
     1801 tcgtggagat cagcttcacg cttcaggccg cggcctgttc cgcggcgcga tgcttgacgt
     1861 cgggcaggaa cccggcgagc agcccgatga gcggcaggaa cgcgcacagc tggtacacgg
     1921 catcg
//
LOCUS       DATLKL010000188         1845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_115021, whole genome shotgun sequence.
ACCESSION   DATLKL010000188 DATLKL010000000
VERSION     DATLKL010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115021"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VM713_02450"
     CDS             complement(<1..320)
                     /locus_tag="VM713_02450"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HVH83154.1"
                     /translation="MDQQPQTTQPPRARRPSGRDAKIAARTARSHAFVPYITRNIPYY
                     EVLSSEGLELLEHNADTILQEIGIDFRDDAEALKLWRDAGADVQGERVRFPRGMCREL
                     VRRSA"
     gene            complement(454..>1845)
                     /locus_tag="VM713_02455"
     CDS             complement(454..>1845)
                     /locus_tag="VM713_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVH83155.1"
                     /translation="TVLMALIDPHPALRAAAPMCPMVDGWKGDDWFHNGAFRQSNFDY
                     IYGQTTRRGEGLKILRGVHDDYQLFLGLGSAGAFAKAFGIDQLPFFAKLSEHPAYDEF
                     WRGQALDQILPAKPLQVPTLFVTSIWDQEDMYGGVHTYAALKSQDKDHKLVFMVLGPW
                     RHSGINGDGTTLGPLKFDGDTALQARRDVIEPFLAEHLKDGAPPANLPPVTVFETGTN
                     VWRRLPAWPLACESGCAARSEPLYLQAHFGLSFTAPPVKSGMPFDEYVSDPAKPVPYI
                     PRPLQFTDRPAWQRWLLTDQRAVEDRTDVLTYTSEPLAAPLHIAGAPMVNLFAATSGT
                     DSDWVVKIIDVYPDEVPANPPLGGYELAIAMDIFRGRYRESLSHPSAVAPGKVQQYRF
                     ALPQVNHVFLPGHRLMVQIQSSWFPLYDRNPQTYVDNIFFARPEDYKKATQRVYHVGG
                     ASSFIDLPVVEQR"
BASE COUNT          297 a          594 c          638 g          316 t
ORIGIN      
        1 gccgagcggc gcacgagctc gcggcacatg ccgcgcggga agcgcacgcg ctcaccctgc
       61 acgtcggcgc cggcatccct ccacagcttg agggcctccg catcgtcgcg gaagtcgatg
      121 ccgatctcct ggagaatggt atcggcgttg tgctcgagga gctcgaggcc ctcgctggag
      181 aggacctcgt agtagggaat attgcgcgtg atatagggaa cgaaggcatg gctgcgcgcg
      241 gtgcgcgccg cgatcttggc gtcgcggccg ctcgggcggc gggcgcgcgg gggctgggtc
      301 gtctggggct gctggtccat gaggctatcc tgaccgtcag gctgtcctga ccgatactga
      361 gtcgcggggg ccctgcccca ttatcgtcag cctcccgggc ggggactggc cggtttgcgg
      421 catcggcttc tccggcagcg ccaccgccgc ggctcagcgt tgctcgacca ccggcagatc
      481 gatgaagctc gacgcgcccc ccacgtggta cacgcgctgc gtcgccttct tatagtcctc
      541 ggggcgcgcg aagaagatgt tgtcgacgta ggtctgcggg ttgcgatcgt agagcgggaa
      601 ccagctcgac tggatctgca ccatgagacg gtgcccgggc aggaatacgt gattcacctg
      661 cggcaacgca aagcggtatt gctgcacctt gccgggtgca acggcgctcg ggtgactcag
      721 gctctcgcgg tagcggccgc ggaagatgtc catcgcgatg gccaactcat agccgcccaa
      781 cggcggattc gcgggcacct catccgggta tacgtcgatg atcttcacca cccagtcgct
      841 gtcggtgccg ctggtcgcgg cgaagagatt caccatgggc gcgcccgcga tgtgcagtgg
      901 cgccgcgagc ggctctgagg tataggtgag cacgtcggta cgatcctcga ccgcgcgctg
      961 atcggtcaga agccaacgct gccacgcggg gcgatcggta aactgcagcg gccgcggaat
     1021 gtagggcacg ggcttggccg gatcggacac gtactcatcg aatggcatgc cgctcttcac
     1081 cggcggcgcc gtgaaactca gcccgaagtg cgcttgcagg tagagcggct cggagcgcgc
     1141 ggcacagcct gactcgcacg cgagtggcca tgccggcagc cttcgccaga cgttggttcc
     1201 ggtctcgaac accgtgacgg gcggcaggtt cgccggcggt gccccgtcct tcaggtgctc
     1261 ggcgaggaag ggctcgatca cgtcgcgccg cgcctgcagc gccgtatcgc cgtcgaactt
     1321 gagcggcccg agcgtcgtgc cgtcgccgtt gatgccgctg tgccgccacg gcccgagcac
     1381 catgaagacg agcttgtggt ctttatcctg cgacttgagc gcggcgtagg tgtgtacgcc
     1441 gccgtacatg tcctcctggt cccagatgct cgtcacgaac agcgtcggca cctgcagcgg
     1501 cttcgcgggc agaatctgat cgagcgcctg gccgcgccag aactcgtcat aggccgggtg
     1561 ctcgctcagc ttcgcgaaga acggcagctg gtcgatgccg aaggctttgg caaaggcgcc
     1621 ggcggatccg aggccgagga acagctgata atcgtcgtgg acgccgcgca ggatcttcag
     1681 cccctcgccg cggcgggtag tctggccgta gatgtaatcg aagttgctct ggcggaaggc
     1741 gccgttgtga aaccagtcat cacccttcca cccatccacc atcggacaca tgggcgccgc
     1801 ggccctgagc gccggatgcg ggtcgatcag cgccatgagc accgt
//
LOCUS       DATLKL010000189         1541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_160109, whole genome shotgun sequence.
ACCESSION   DATLKL010000189 DATLKL010000000
VERSION     DATLKL010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160109"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VM713_02460"
     CDS             complement(<1..1097)
                     /locus_tag="VM713_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83156.1"
                     /translation="MPEVASQTLELVGREVTATLAEARGALENYVEQPDNVPLLERCA
                     QDLHQVQGVLRVLEIYGAALLAEEMEHVAQYLIATVNERKGQAESLDALMRAMMQLPS
                     YLERVLAGGRDLALVLLPLLNDLRAVRGHALLSEGTLLLLNLKSDKQAQPQPVTAGEA
                     PMTVEQWARRLRARYQQGLIGWIRGERPEQNLEVLAAVAHKLEQIATRQPVFQLWWVI
                     GAVIEALQHNGLEGGQSVKRLLGLADREMRRLYEQGEARYAQTPPIELLNNLLYYVGR
                     AESNGGRVTAVRASFRLAELLPVDESIEQERENLSAPSVKLMQTVAAAIREDLGRVKD
                     GFASCDRSRRNETRRSSGGHVLRRGRVRVTLV"
     gene            complement(1113..>1541)
                     /locus_tag="VM713_02465"
     CDS             complement(1113..>1541)
                     /locus_tag="VM713_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83157.1"
                     /translation="IQTDTNEAVVSMERSTTDVVGGALLAENAGAALEEIEQVSNQIA
                     SLVQNISSSARAQTSAAQGIARNMQVLKEISAQTAESTNATSISIAKLAELSAGLRKS
                     AAGFRLPGSVATGSSVRLKKLEESTLTTAKVRQISALGGS"
BASE COUNT          208 a          544 c          527 g          262 t
ORIGIN      
        1 acgagggtta cccgaaccct tccacgcctc agtacgtgcc caccgctcga gcgccttgtc
       61 tcgtttcgcc tcgagcgatc gcaacttgcg aagccatcct tgacgcgccc gaggtcctcg
      121 cggatcgccg cagccacggt ctgcatgagc ttcacagaag gcgccgagag gttctcgcgc
      181 tcctgctcga tgctctcgtc caccggcagc agctccgcca ggcggaacga ggcgcgcacg
      241 gcggtcaccc ggccgccgtt ggactccgcg cggccgacgt aatagaggag attgttcagc
      301 agctcgatcg gcggcgtctg cgcatagcgc gcttcgccct gctcgtagag gcggcgcatc
      361 tcgcgatcgg cgagccccag gagcctcttg accgactggc caccctcgag cccgttgtgc
      421 tgcagcgcct cgatgaccgc gccgatcacc caccacagct ggaataccgg ctggcgggtg
      481 gcaatctgct cgagcttgtg ggccacggcg gcgagtactt cgaggttctg ctcgggccgc
      541 tcgccgcgga tccagccgat gagcccctgc tggtagcggg cgcgcagccg gcgtgcccac
      601 tgctcgacgg tcatcggcgc ctcgccggcg gtgacgggct gcggctgcgc ctgcttgtcg
      661 gacttcaagt tgagcagcag cagcgtgccc tcggagagca gcgcatggcc gcgcaccgcg
      721 cgcaggtcgt tgagcagcgg cagcagcacc agcgcgagat cgcgcccgcc ggcgagcacg
      781 cgctcgaggt agctcggcag ctgcatcatg gcgcgcatca gggcatcgag gctctccgcc
      841 tgccccttgc gctcgttgac cgtggcgatc aggtactgcg ccacgtgctc catctcttcg
      901 gcgagcagcg ccgcgccgta gatctccagc acccgcagca cgccctgcac ctggtgcagg
      961 tcctgcgcgc agcgctcgag cagcggcacg ttgtcgggct gctcgacgta gttctcgagc
     1021 gcgccgcgcg cctcggcgag cgtggcggtg acctcgcggc ccactagctc gagtgtctgg
     1081 gatgcgactt cgggcatgag gcgggcttac cttcaggagc cgccgagcgc ggagatctgg
     1141 cgcaccttgg cggtggtgag cgtgctctcc tcgagcttct tcaggcgcac gctgctgccg
     1201 gtggcgacgc tcccgggcag gcggaagccc gccgccgact tgcgcagccc ggcggagagc
     1261 tccgcgagct tggcgatcga gatcgaggtc gcgttggtgg actcggcggt ctgcgcactg
     1321 atctccttca ggacctgcat gttgcgggcg atgccctgcg ccgcgctcgt ctgggcgcgg
     1381 gcgctcgagg agatgttctg caccaggctc gcgatctggt tcgacacctg ctcgatctcc
     1441 tcgagcgcgg cgccggcgtt ctcggcgagc agcgcgccgc cgaccacgtc ggtggtactg
     1501 cgctccatcg agacgaccgc ctcgttggtg tcggtctgaa t
//
LOCUS       DATLKL010000190         3662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_24759, whole genome shotgun sequence.
ACCESSION   DATLKL010000190 DATLKL010000000
VERSION     DATLKL010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24759"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1775)
                     /locus_tag="VM713_02470"
     CDS             complement(9..1775)
                     /locus_tag="VM713_02470"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HVH83158.1"
                     /translation="MRDRGLSAIVAGVLAPLVLVLCCRAVPAASPSAAAPPDYDVVIR
                     GGTVYDGSGSAPFVGDVAIRGERIAYVGVHAPGRSAEEFQARGKAVAPGFINMLAHPE
                     ESLLIDGRALSDLRQGVTLEVLGEDSMGPLSAEMKRLALQREADIKYDIDWTTLGEYL
                     TNLERRGIAPNVASYVGAGTVRTYVLGERDVQPTAEQLAEMRQLVHEAMEQGALGVTT
                     ALIYSPNQYAKTPELIALAQESARCGGIYSAHMRSEGDRLLEGVQETVAIARASGAPA
                     EIYHLKEAGKRNWDKLDALILAVQAARASGVRITADMYVYTAAATGLDAAMPPWVQDG
                     GLEAWIARMKDPATRARLIADMRDPSPAWENVLGAAGPRGTLLLGFKNPALKPLTGKT
                     LAAVATARGVSAQDAAIDLVIEDGSRVGVAYFLMSEDNVRREVALPWVSFDSDADAPA
                     PDGVFLKSSRHPRTYGNFARVLAHYVREEKVITLQAAIHKLAALPAATLSLSDRGLLR
                     QGYFADIVVFDPAKVQDHSTYQRPQQLATGVGDVWINGVRALRHEVATGAASGRAIRG
                     RAWTGCGGGGCRNSGADWSWSP"
     gene            complement(1772..3085)
                     /locus_tag="VM713_02475"
     CDS             complement(1772..3085)
                     /locus_tag="VM713_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH83159.1"
                     /translation="MSRGEVIVVGGGLAGLASAYALHEAGYAVRVLERREDVALETSF
                     ANGGILTPSMPDPWNAPGVHWQLLRWLGREDAPMLLRPSRVLSYLGWGLQFLAASAPR
                     SYEASMRANYRLCAYSLERLRTWRAVLGLEYAAGTHGTIEVYRDPGSFAHAVAEVRSL
                     SSLGLVGTPLDPAGTARLEPLLDEIRADLAGAIHYPADESGDALLFCRALRERLEARG
                     VKVSCTTPVRAVRVRAGRVAGVETGGGFVPGSDVVLAAGPWSRALLAACGVRVQVQPV
                     KGYSLSIPIDPRAAPRHALSDDSLHAVMTPLGPTLRLAGTAEFSGWDTRLLQGRVESL
                     WRLLAALNPTLARTVARSSARPWCGLRPMSADGRPYIGATPVAGLYVNTGHGHLGWTQ
                     AAGSAALLAQLMSGAPTAIDPRPFALSRTERRAWRGEVLPTEQHS"
     gene            complement(3095..>3662)
                     /locus_tag="VM713_02480"
     CDS             complement(3095..>3662)
                     /locus_tag="VM713_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253063.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HVH83160.1"
                     /translation="NGRQGVRYALQEGMTVLDPETMQEVPANGEVLGEIMFRGNIVMK
                     GYLKNPQATAAVFAGGWFHSGDLGVLEPDRYVKIRDRSKDIIISGGENISSLEVEDAL
                     YRHPAVMVCAVVARPDPKWGESPLAYVELKSGATASAEELIAHCRSLLAGFKVPREVR
                     FEEIPKTATGKIQKFVLRERVRSASAIE"
BASE COUNT          553 a         1319 c         1275 g          515 t
ORIGIN      
        1 gactcaggtc agggtgacca gctccagtcg gcgccgctgt tgcggcagcc gccgcctccg
       61 cagcccgtcc aggcgcgccc gcggatcgcc cggccgctgg ccgcgccggt cgccacctcg
      121 tggcggagcg cgcgcactcc attgatccag acatcgccga ccccggtcgc gagctgctgc
      181 gggcgctgat aggtggagtg gtcctggacc ttcgccggat cgaacaccac gatatcggcg
      241 aagtaaccct ggcgcagcag gccgcgatcg ctgagcgaga gggtcgccgc cggcagcgcg
      301 gccagcttgt ggatcgccgc ctgcagggta atgactttct cctcacgcac gtagtgcgcc
      361 agcacccgcg cgaagttgcc gtaggtgcgc ggatggcggc tcgacttgag gaacacgccg
      421 tccggcgccg gtgcgtcggc atccgaatcg aagctgaccc agggcagagc cacctcgcgc
      481 cgcacgttgt cctccgacat gaggaaatag gcgacgccga cgcgcgagcc gtcctcgatg
      541 acgaggtcga tcgcagcgtc ctgcgcgctc acaccgcgcg cggtggcaac cgccgcgagc
      601 gtcttgccgg tgagcggctt gagcgcggga ttcttgaagc cgagcagcaa cgtcccgcgg
      661 ggaccggcgg cgccgagcac gttctcccag gcgggcgagg gatcgcgcat gtcggcgatc
      721 agacgggcgc gcgtcgccgg atccttcatg cgcgcgatcc acgcctcgag cccaccgtcc
      781 tgcacccagg gcggcatggc ggcatcgagc ccggtcgcgg ccgcggtata gacatacatg
      841 tcggcggtga tgcgcacccc cgaggcgcgc gccgcctgca ccgcaaggat cagggcgtcg
      901 agcttgtccc agttgcgctt gccggcctcc ttcaggtgat agatctcggc cggagcgccc
      961 gaggcgcgcg cgatggcgac ggtctcctgc accccctcca gcagccggtc gccttcgctg
     1021 cgcatgtgcg cgctgtagat gccgccgcag cgcgccgact cctgcgcgag cgcgatgagc
     1081 tcgggcgtct tggcgtactg gttggggcta tagatgagcg cggtcgtcac cccgagggcg
     1141 ccctgctcca tggcctcgtg caccagctgg cgcatctcgg cgagctgctc ggcggttggc
     1201 tgcacgtcgc gctcgccgag cacgtaggtg cgcaccgtgc ccgcgccgac gtaggaagcc
     1261 acattgggag cgatgccgcg cctctcgagg ttggtgaggt attcaccgag cgtcgtccag
     1321 tcgatgtcgt acttgatgtc tgcctcccgc tggagcgcga ggcgcttcat ctccgcggac
     1381 agcgggccca tggagtcctc ccccaggacc tcgagcgtga ctccctggcg cagatcactg
     1441 agcgcgcgcc catcgatcag cagcgactcc tccgggtgcg ccagcatgtt gatgaagccc
     1501 ggcgccactg ccttgccgcg cgcctggaac tcctcggcgc tgcgcccggg cgcgtgcact
     1561 ccgacgtaag cgatgcgctc gccgcggatc gcgacatcgc ccacgaacgg cgcgcttccc
     1621 gatccgtcgt agacggtgcc gccacggatc accacgtcgt agtcgggtgg cgccgcggcg
     1681 gacggcgacg ccgccggcac ggcgcgacag cagagcacca gcacgagggg cgcgagcacg
     1741 cctgccacaa tcgcggagag tcctcgatct ctcatgaatg ttgctccgtt ggcaggacct
     1801 cgccgcgcca ggcgcgccgc tcggtacgcg acagcgcgaa gggccgcgga tcgatggcag
     1861 tcggtgcgcc ggacatcagc tgcgcgagga gcgcggccga gccggccgcc tgcgtccagc
     1921 cgagatggcc gtgcccggtg ttgacgtaga gccccgcgac cggcgtcgcg ccgatgtagg
     1981 gacgaccgtc ggcggacatc ggacgcaacc cgcaccaggg ccgcgcgctc gagcgcgcga
     2041 cggtgcgcgc gagcgtggga ttcagggccg cgagcagccg ccacagcgac tcgacccggc
     2101 cctggagcag ccgtgtgtcc cagccgctga actcggcggt gccggcgagc cgcagcgtgg
     2161 gcccgagcgg cgtcatcacc gcgtgcagtg aatcgtccga cagcgcgtgc cgcggggctg
     2221 cacgcggatc gatcgggatc gagagcgaat aacccttcac cggctgcacc tgcacgcgca
     2281 ccccgcaggc ggcaagcagc gcgcgggacc atgggcccgc ggccagcacg acgtccgagc
     2341 ccggcacgaa gccgccgccg gtctccactc ctgcaacgcg gccggcgcgc acccgcacgg
     2401 cgcgcaccgg cgtggtacag ctcaccttga cgccgcgcgc ctcgagccgc tcgcgcagcg
     2461 cccggcagaa aagcaaggca tcgccactct cgtccgccgg atagtggatg gcgccggcga
     2521 gatcggcgcg tatctcatcg aggaggggct cgagccgcgc ggtgccggcc gggtcgagcg
     2581 gcgtgcccac gaggccgaga gagctgagcg agcgcacttc ggccacggcg tgcgcgaacg
     2641 agcctgggtc gcgatacacc tcgatcgtcc cgtgggtacc cgcggcgtac tcgagaccga
     2701 ggaccgcgcg ccacgtgcgc agccgctcga ggctataggc gcacaggcgg tagttggccc
     2761 gcatcgaggc ctcataggag cgcggcgcgg aagccgccag gaattgcagg ccccagccca
     2821 gatagctcag aacgcgtgag ggccgcagca gcatcggcgc gtcctcccgc ccgagccagc
     2881 gcagcagctg ccagtgcacg ccgggcgcgt tccaggggtc cggcatcgag ggtgtcagga
     2941 tcccgccgtt ggcgaagctc gtctcgagcg cgacgtcctc gcgccgctcg agcacgcgca
     3001 ccgcgtagcc cgcttcatgg agcgcgtagg cgctggcaag gcccgccagc ccaccgccca
     3061 ccacgatgac ctcgccgcgg ctcatggcgt gcggctactc gatcgcgctg gcggaccgga
     3121 cgcgctcgcg cagcacgaac ttctggatct tgccggtggc ggtcttcgga atctcctcga
     3181 agcgcacctc gcgcggcacc ttgaagccgg cgaggaggct ccggcaatgg gcgatcagct
     3241 cctcggcgct cgccgtggcg ccgctcttga gctcgacgta ggcgagcggc gactcgcccc
     3301 acttgggatc cggccgcgcc acgaccgcgc ataccatgac ggccggatgc cggtagaggg
     3361 catcttcgac ttcgagcgag ctgatgttct caccgccgga gatgatgatg tccttgctgc
     3421 ggtcacggat cttcacgtag cggtcgggct cgaggacgcc gagatcgccg ctgtgaaacc
     3481 agccgcccgc gaatactgca gcggtcgcct gcgggttctt gaggtagccc ttcatgacga
     3541 tgttgccgcg aaacatgatc tcacccagca cctcaccgtt cgccggcacc tcctgcatcg
     3601 tctcggggtc gagcacggtc atgccctcct gcaacgcgta acgcacgccc tggcggccgt
     3661 tg
//
LOCUS       DATLKL010000191         2176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_171367, whole genome shotgun sequence.
ACCESSION   DATLKL010000191 DATLKL010000000
VERSION     DATLKL010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171367"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VM713_02485"
     CDS             complement(<1..356)
                     /locus_tag="VM713_02485"
                     /inference="COORDINATES: protein motif:HMM:NF027404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HVH83161.1"
                     /translation="MLEPTPLYGGNADFLDALYEQYLRDPASVEERWRSYFTQLEPPG
                     GEQPHGPIRDAIVARAAMPRAAAGMPAAAPSPDARQAAVSRLIQVWINRGHLVATIDP
                     LALTARPRPRALELEH"
     gene            complement(449..928)
                     /locus_tag="VM713_02490"
     CDS             complement(449..928)
                     /locus_tag="VM713_02490"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HVH83162.1"
                     /translation="MSTEPHDPALWGDPPPERRHHERRQQVLRALLLGSFRTRRRQPR
                     RAGEQALAAVDWHHPQWLATAILIVLLSSADALLTLTLIEHGAYETNPLMRPLVYGSA
                     LPFTVVKVALTAGGVVLLTLLASMRLFGRLSAGLMLYALLGGYGVLILYECKMLDLF"
     gene            1057..>2176
                     /locus_tag="VM713_02495"
     CDS             1057..>2176
                     /locus_tag="VM713_02495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH83163.1"
                     /translation="MRRMSSSSSASALRASAPDGLPVPRRYWAIAAILLSICMSVLDS
                     TITNVALPTIARDFHASSAASIWVINAYQLAILVVLLPLAALGEVIGYRRVSQAGLWV
                     FTLASLGCALAPSLLTLSIARVIQGLGAAGIVSVNAALVRFTYPHRYLGRAIGINAFV
                     VATSSALGPTIASAVLAVAQWRWLFGINVPLGIITILIARYSLPDAERAQRPLNLASA
                     VLYAGTFGLLLAGLQSLAHHSATPLALTQILGGAVLAVVLVRHELPLRAPLIPFDLMR
                     VRLFRLSLAASTCAFIGQMSALVSLPFEIQRLGHTAVETGLYMTPWPVALALTAPVAG
                     RLADRYPAGLLGGLGLLVMSTGLALLGFFPANGSAWGFV"
BASE COUNT          296 a          790 c          722 g          368 t
ORIGIN      
        1 tagtgctcga gctcgagggc gcgcggccgc ggccgggccg tgagagcgag cggatcgatg
       61 gtcgcgacga ggtggccgcg gttgatccag acctggatga gccgggagac cgccgcctgc
      121 cgggcatccg gcgaaggggc cgcggcgggc atgccggccg cggcgcgcgg catggccgcg
      181 cgcgccacga tggcgtccct gatgggcccg tggggctgct cgccccccgg gggctccagt
      241 tgcgtgaaat agctgcgcca gcgctcctct acgctcgcgg gatcgcgcag gtattgctcg
      301 tagagggcgt cgagaaagtc ggcgttaccg ccgtagaggg gtgtgggttc gagcatgagg
      361 gggaaaacac tcggcgaccc agtagtttaa cggaaagctc gccggcgggg aaaagccggt
      421 ctggccgctg gccaccggcc gccgcggctc agaacaggtc gagcatcttg cactcgtaga
      481 ggatcaacac cccgtatccg ccgagcagcg catacagcat gagccctgcc gacagccggc
      541 cgaacaaccg catgctcgcc agcagtgtga gcagtaccac cccaccggcg gtaagcgcca
      601 ccttcaccac cgtgaagggg agcgccgagc cgtagaccag gggccgcatc agcgggttgg
      661 tctcgtaagc gccgtgctcg atgagcgtca gggtaaggag cgcatcggcg ctggaaagca
      721 gcacgatcag aatcgccgtg gcgagccatt gcggatgatg ccagtcgacc gcggcgagtg
      781 cctgctcgcc cgcacgcctg ggctggcggc gccgcgtgcg aaagcttccc agcagcagcg
      841 cccgcagcac ttgctggcga cgctcgtggt ggcgccgctc gggcggcggg tcgccccata
      901 gggctggatc gtgcggctcg gtggacacgt ttgttgttgt tgtcaggctc gcgatgggct
      961 gactccattg tagccgcacc ccgccgccca cagtcactcc ggaaaaccct gtagcgcgcg
     1021 ccagcttatg acacctggcg ctgccccctc agtatcatgc ggcggatgtc gagctcgagc
     1081 tccgcctccg cgctacgcgc gagcgccccc gacggcctgc cggtaccgcg ccgttactgg
     1141 gcgatcgcgg ccatcctgct gtcgatctgc atgtcagtgc tcgacagcac catcaccaac
     1201 gtcgcgctgc cgaccatcgc gcgtgatttc cacgccagct ccgccgcctc gatctgggtc
     1261 atcaatgcct accagctggc gatcctcgtc gtgctgctgc cgctcgccgc gctcggcgag
     1321 gtcatcggtt accggcgcgt ctcgcaggcc gggctgtggg tgtttacgct ggcctccctc
     1381 ggctgcgcgc tcgcgccctc gctcctcacg ctgagcatcg cccgggtcat ccaggggctc
     1441 ggcgcggcgg ggatcgtgag cgtcaacgcg gcactggtgc gcttcaccta cccgcaccgc
     1501 tatctcggcc gcgccatcgg catcaacgcc ttcgtagtcg caacctcatc ggccttgggt
     1561 cccacgatcg catcggcggt gctcgcggtg gcgcagtggc gctggttgtt cggaatcaac
     1621 gtgcctctcg ggatcatcac catcctgatc gcccgctact cgctccccga tgccgagcgc
     1681 gcccagcggc cgctcaacct cgccagcgcg gtgctctacg ccggcacctt cgggctgttg
     1741 ctcgcggggc tgcagtctct cgcgcaccat tcggcgaccc ccctcgcgct cacgcagatc
     1801 ctgggcggcg ccgtgctcgc cgtcgtgctg gtgcgtcacg agcttccgct ccgcgcaccg
     1861 ctcatcccct tcgacctgat gcgcgtgcgg ctcttcaggc tctcgctggc cgcctccacc
     1921 tgtgccttca tcggccagat gagcgcgctc gtgtcgctgc ccttcgagat ccagcgcctc
     1981 gggcacaccg ccgtcgagac cgggctttac atgacgccct ggccggttgc cctggcgctc
     2041 accgcgccgg tcgccggccg cctcgccgac cgctaccctg ccggactcct cgggggcctg
     2101 gggctcctgg tgatgtccac cggactcgcg ctcctcggct ttttccccgc aaacggcagc
     2161 gcctggggct tcgtct
//
LOCUS       DATLKL010000192         2041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_193901, whole genome shotgun sequence.
ACCESSION   DATLKL010000192 DATLKL010000000
VERSION     DATLKL010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193901"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..828
                     /locus_tag="VM713_02500"
     CDS             19..828
                     /locus_tag="VM713_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83164.1"
                     /translation="MDTRLRPSRAAFAAALIGLGILGLIYGNSSEIWESIPRSLPGRS
                     LVIYLCSLIALGTGVGLLRGSSAGFACRILLPFLLLWLVLLKLPPLLSAPRVVVNWES
                     FAETATLCAGGWCLFAACARPWEQRHLGLTVGARGIRIARLLLIAALPMIGVSHFAYA
                     ELTAGLVPKWLGFPVGWTYLTGAASLAASAGMLFAILPRLAASLEAAMLWIFTLLVWV
                     PRVAASPHRQGDWSELLISAAIASGAWLVAETYRGVPWLSSGRAARAITLG"
     gene            853..1176
                     /locus_tag="VM713_02505"
     CDS             853..1176
                     /locus_tag="VM713_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83165.1"
                     /translation="MRATSNIPALGRWRLWFGALAVLEFLWFLVLRPYFSGSFFSHPK
                     LMLPPLTVVGYVYLLGAVSICLESLEWDYRMRQLIVIMLGLSVGLFVFALMTLTTAQL
                     EGEAG"
     gene            1187..1480
                     /locus_tag="VM713_02510"
     CDS             1187..1480
                     /locus_tag="VM713_02510"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HVH83166.1"
                     /translation="MAKAYWIAAYRSVRDPQAFAAYAKLALPALEAAGRRFLVRGMPA
                     RVYESGLEQRTVLIEFDGVAQAIAAHESPAYQEALRVLGNGADRDMRIVEGVG"
     gene            1534..1992
                     /gene="aac(6')"
                     /locus_tag="VM713_02515"
     CDS             1534..1992
                     /gene="aac(6')"
                     /locus_tag="VM713_02515"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: protein motif:HMM:NF043067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047663 - aminoglycoside
                     6'-N-acetyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6'-N-acetyltransferase"
                     /protein_id="HVH83167.1"
                     /translation="MAEWLLAPPQGSDDRAWLGLRIALWPDGSEAEHLSAMAETLARG
                     HYVRLALTASGSAVGFVEASRRVDYVNGTTSSPVAFLEGLYVAPGARRQGAARALVDS
                     VVTWALHQGLSELASDSLIDNVAAHAAHRALGFEETERVAYFRRPLQLLP"
BASE COUNT          257 a          713 c          677 g          394 t
ORIGIN      
        1 gcatcgaagt agccccccat ggacacccgc ctccggccga gtcgtgcagc cttcgccgcc
       61 gcgttgatcg gcctcggtat cctcggcttg atctacggga actcctctga gatctgggag
      121 agcattccga ggagcctgcc cggccgttcc ctggtcattt acctctgctc gctcatcgcg
      181 ctcgggaccg gtgtcgggtt gctgcggggt tcgtccgccg gcttcgcgtg ccgcattctg
      241 cttcccttcc tgctgctctg gttggtgttg ctgaagctcc caccgctgct atcggccccg
      301 cgggtcgtgg tgaactggga gtcgttcgcc gagacggcca cgctctgcgc cgggggctgg
      361 tgcctgttcg ccgcctgcgc acgcccctgg gagcagaggc atctcgggct caccgtcggc
      421 gcgcgcggga tccgcatcgc ccggctgctg ctcatcgccg cgttgcccat gatcggcgtg
      481 agccacttcg cctacgccga gctgaccgcg ggcctcgtgc cgaagtggtt gggcttcccc
      541 gtcggctgga cgtacctgac cggcgctgcg agtctcgcgg cctcggcggg catgttgttc
      601 gccatcctgc cgcgtctcgc ggcaagcctc gaggccgcga tgctgtggat cttcacgctg
      661 ctcgtgtggg tgccacgcgt cgcagcaagc ccccaccgtc agggggactg gtcggagctg
      721 ctgatttccg ccgcgatcgc cagcggcgcc tggctggtgg ccgagaccta ccgcggtgtc
      781 ccctggctct cgagtggcag ggcggcgcgc gcgataaccc tcggctgagg atacggaggc
      841 aaggatcgac ccatgcgagc cacttcaaac atcccggcgc ttggccgctg gcgtctctgg
      901 ttcggcgctc tcgccgtgtt ggagtttctc tggttcctcg tgctgcgccc gtacttctcc
      961 gggagcttct tcagtcatcc gaagctgatg ctgcctccgc tcaccgtggt cggctacgtt
     1021 tacctccttg gggccgtctc catctgcctg gagagcctcg agtgggatta ccggatgcgc
     1081 cagctcatcg tcatcatgct cgggctcagc gtggggctgt tcgtgttcgc gctgatgacg
     1141 ttgacgacgg cacaactcga gggcgaagcc ggctgagggt tcaaccatgg ctaaagcata
     1201 ttggatcgct gcctaccgct cggtacgcga cccgcaggca ttcgcggcct acgccaaact
     1261 ggctcttccg gcgctcgagg cggccggcag gcgcttcctg gtgcgcggca tgccggcgcg
     1321 cgtctatgaa agcgggctgg agcagcgcac cgtcctgatc gagttcgacg gcgtcgcaca
     1381 agccatcgcc gcgcacgaga gccccgcgta tcaggaggcg ttgcgtgtgc tcggcaatgg
     1441 cgccgatcgt gatatgcgca tcgtcgaagg cgttggctaa gtcggaccgc gcgccacccg
     1501 ccgcgctcct cgagcgcgcg aggcgcggca cccgtggctg aatggctcct cgcaccgccg
     1561 cagggcagtg acgaccgcgc ctggctcggc ctgcggatcg cgctctggcc tgacggctcg
     1621 gaggccgagc acctctccgc catggccgag acgctcgccc gcggccatta cgtgcgcctg
     1681 gcgctcaccg cctcgggctc cgccgtgggt tttgtagagg cgtcgaggcg cgtcgactat
     1741 gtcaatggca cgacgagctc cccggtggca ttcctcgagg gtttgtacgt cgcgccggga
     1801 gcgcgccggc agggcgcggc acgcgcgctc gtggactccg tcgtgacctg ggcgctgcac
     1861 caaggcttaa gcgagctcgc ctccgactcg ctcatcgaca atgtcgcagc gcacgccgcc
     1921 caccgcgcgc tcggatttga ggaaacggag cgcgtggcgt actttcgtcg cccgctccag
     1981 ctgctgccat gaccatcgcc acaccactgc tcatcctcgt gctactcgcg ctgtcgggct
     2041 t
//
LOCUS       DATLKL010000193         3464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_58605, whole genome shotgun sequence.
ACCESSION   DATLKL010000193 DATLKL010000000
VERSION     DATLKL010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58605"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VM713_02520"
     CDS             <1..727
                     /locus_tag="VM713_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVH83168.1"
                     /translation="QKVLAALLYPIVLSVMCFAIVSGLLVFMVPKVIAVFESTKGKLP
                     LITRLLIATSDFLRHYGLYLAIALALAVWLFRRWQRDPAAKRRLQRFLLTVPIVGKLS
                     RGFNTARFTRTFSILSASAVPVLEALRIAGEVVTNLPMRDAVADAAARVREGAPIGRS
                     LAVSRMFPPMTIHLISSGESSGELESMLERAAIGQERELDALLAAMVGLLGPLLIILM
                     GAFVMGIVFAMLLPIFEINNLIH"
     gene            854..1126
                     /locus_tag="VM713_02525"
     CDS             854..1126
                     /locus_tag="VM713_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83169.1"
                     /translation="MSEKPESDEFEDEEADEPVIGDGDVDLDQVIKDLDLAKRRGQKA
                     GDPAWRRLERLLEDKRTAELTSDFEDYEIGEDGESPRKGRRRPRNS"
     gene            complement(1148..2902)
                     /locus_tag="VM713_02530"
     CDS             complement(1148..2902)
                     /locus_tag="VM713_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVH83170.1"
                     /translation="MSAPRTRVLVADDDGMLREIATAMLENAGCEAEAVASGNAAVAV
                     CAARMPDLVLLDVDMPEGNGYQACSGIRALPGGGEVPIVTVTGFDDAASINLAYDAGA
                     TDFVVKPINWPLLAHRMRYVLRGARAQAHIHRLAYFDGLTGLPNREWIGEYLSRSLAV
                     AAQSERGVAMLFVDLDQFKRINDTLGHETGDALLRQVAARLSSALAYAGADAQLARVG
                     GDEFIVVLTGNARVGEAEQAAQRVQAALTKPFVHGDYEFVVTPSIGIALYPEHGVDGQ
                     NLRKNADLAMYEAKAGGRNQYRLYSSAINTRAVKRLSLEMELRRAFEARQLEVYYQPQ
                     YDAHSLELAGAEALLRWFHPQRGQISTADFIAVAEETGLIGDIGRWTLEQVCRDLRHW
                     RGAGLAVPAISVNVSGRDFMRQDVLLRLSRVVEEARLPASLFELELTEGVLMQDVESG
                     QRSLSALKEYGFALAVDDFGTGYCSLNYLKRFPLDTLKVDRAFVNDITADPDDATIVR
                     AIVGLGHNLNLKIVAEGVETLDQLQFLRATGCDLVQGYLMSRAVDALAFTQLLRMSTP
                     APQAAPATARRDAAGGGT"
     gene            complement(2899..>3464)
                     /locus_tag="VM713_02535"
     CDS             complement(2899..>3464)
                     /locus_tag="VM713_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HVH83171.1"
                     /translation="TADVTREGRSACLAAGMDDHLGKPFTREALHAMLRRWLPAPRAL
                     ATAAAGTLGAAAEAATVPPAARAPAAELLDRGTLEALRALPARGAESMLSRIAATYLS
                     DSERLLDTIERAVAAGQAAELARAAHAWASCNGNVGALGLMNLCRELESRARAGDLSS
                     ARALLTLLRDGYSSVGEELRGELRRSA"
BASE COUNT          540 a         1194 c         1180 g          550 t
ORIGIN      
        1 ccagaaggtg ctggcggcgc tgctataccc gatcgtgctc tcggtgatgt gctttgcaat
       61 cgtgtcgggc ctgctggtgt tcatggtgcc gaaggtgatc gcggtattcg agtcgaccaa
      121 aggtaagctc ccgctgatca cgcgtctcct gatcgccacc agcgacttcc tgcgccacta
      181 cgggctctat ctcgccatcg cgctggcgct cgccgtgtgg ctgttccggc gctggcagcg
      241 cgatccggct gccaaacggc gcctgcagcg cttcctcctc acggtgccga tcgtcggcaa
      301 gctctcgcgc ggcttcaaca ccgcgcgctt cacccgcacc ttcagcatcc tctcggcgag
      361 cgccgtgccg gtactcgagg cgctgcgcat cgcgggcgag gtggtcacga acctgccgat
      421 gcgggatgcg gtggcggatg ccgcggcgcg cgtacgcgag ggcgcgccca tcgggcgctc
      481 gctggcggtg agtcgcatgt ttccgcccat gaccatccac ctgatctcga gcggcgagtc
      541 gagtggggag ctcgagagca tgctggagcg tgccgccatc ggccaggagc gtgagctcga
      601 cgccctgctg gccgccatgg tggggctcct cggaccgttg ctgatcatcc tgatgggcgc
      661 gttcgtcatg ggcatcgttt ttgcaatgct gctgcccatc ttcgagataa ataacctgat
      721 ccactgacgt tcgttgacgt gccggggcca cagaccgggc acggttcaca aaaatcacac
      781 ctgcgacatt gcagttgcag cgggatctga tttaagttcg cggcgtccta ttcctgcccg
      841 acagctgtcc cgcatgagcg agaagccgga atcagacgaa tttgaggacg aagaggccga
      901 cgagccggtc atcggcgacg gtgacgtcga tctcgaccag gtgatcaagg acctggacct
      961 tgccaagcgc cgcggccaga aagcgggcga cccggcgtgg cggcgcctcg agcggctgct
     1021 cgaggacaag cgcaccgccg agctcaccag cgacttcgag gactacgaga tcggcgagga
     1081 cggggagagt ccgcgcaagg gccggcgcag gcctcgcaac agctgaggag ggccgcggcg
     1141 cctcgaatca ggtcccgccg cccgcggcgt cgcgccgcgc cgtggcgggg gccgcttgcg
     1201 gcgccggcgt cgacatacgc agcagctgcg tgaacgccag ggcatcgacc gcccggctca
     1261 tgagatagcc ctgcaccagg tcgcagcccg tggcacgcag gaactgcaac tggtcgagcg
     1321 tctcgacgcc ctcggccacg atcttgagat tgaggttgtg cccgagtccg acgatcgcgc
     1381 gcacgatggt ggcatcgtcc ggatcggccg tgatgtcgtt gacgaacgcg cggtccacct
     1441 tgagggtgtc gagcgggaag cgcttgaggt agttgagcga gcagtagccg gtcccgaagt
     1501 cgtcgaccgc cagcgcgaag ccgtattcct tgagcgcgga gagcgagcgc tggccgctct
     1561 cgacgtcctg catcaggact ccctcggtga gctccagctc gaacagcgag gccgggagtc
     1621 gtgcctcctc gaccacgcgc gacagccgca gcagcacgtc ctggcgcatg aagtcgcggc
     1681 ccgagacatt caccgagatc gccggcaccg cgagcccggc gccgcgccag tgtcgcaggt
     1741 cgcggcacac ctgctcgagt gtccagcggc cgatgtcgcc gatgagcccg gtctcctcgg
     1801 cgaccgcgat gaagtcggcc gtggatatct ggccgcgctg cggatgaaac cagcgcaaca
     1861 gcgcttccgc gcccgcgagc tcgaggctgt gggcgtcgta ctgcggctgg tagtaaacct
     1921 cgagctgtcg cgcctcgaac gcccgccgca gttccatctc gagcgacaac cgcttcaccg
     1981 cccgtgtgtt gatcgcactg ctgtacaggc ggtactgatt gcggccgccg gccttggcct
     2041 cgtacatggc gaggtccgcg ttcttgcgca aattctgccc gtcgacgccg tgctcggggt
     2101 agagcgcgat gccgatgctc ggggtcacga cgaactcgta atcgccgtgg acaaacggct
     2161 tcgtgagtgc cgcctgcacc cgctgtgcgg cctgttcggc ctcgccgact ctggcgttgc
     2221 cggtgagcac gacgatgaac tcgtccccgc ccacacgcgc gagctgagcg tccgccccgg
     2281 cataggcgag cgctgagctc agccgggcgg cgacctggcg cagcagcgca tcgccggtct
     2341 cgtgacccag agtgtcgttg atgcgcttga actgatcgag gtcgacgaac agcattgcga
     2401 ccccgcgctc gctctgcgca gccacggcga gcgagcgcga caggtactcg ccgatccact
     2461 cgcggttggg caacccggtg aggccgtcga agtacgccag tcggtggatg tgcgcctggg
     2521 cgcgcgcccc gcgcagcacg tagcgcatac gatgcgcgag cagcggccag ttgatcggct
     2581 tcaccacgaa gtccgtcgcc ccggcgtcgt aggccaggtt gatcgaggcg gcgtcgtcga
     2641 agcccgtcac cgtgacgatc ggaacttcgc cgccgccggg aagcgcgcga atgccgctgc
     2701 aggcctggta gccgttgcct tcgggcatgt ctacatccag cagcaccaga tccggcatgc
     2761 gcgctgcgca cactgcaacc gccgcattgc cgctggcgac ggcctcggcc tcgcagccgg
     2821 cattctcgag catcgcggta gcgatctcgc gcagcatccc gtcgtcgtcg gcgaccagca
     2881 cgcgggtgcg cggcgcgctc acgcggacct ccgcagctcg ccgcgcagct cctcgccgac
     2941 gctgctgtat ccatcgcgta gcagcgtgag cagggcacgt gccgacgaca gatcgccggc
     3001 gcgtgcgcgg ctctcgagct cgcgacacag gttcatgagt ccgagcgccc ccacgttgcc
     3061 attgcacgac gcccaggcat gggcagcccg cgcgagctcc gcggcctgac ccgccgcgac
     3121 ggcccgctcg atcgtgtcga gcaaccgctc cgagtcggac agataggtgg cggcgatccg
     3181 gctcagcatg ctctccgcac cgcgtgccgg gagcgcccgc agcgcttcga gcgtgccccg
     3241 gtcgagcagc tccgcggccg gagcgcgcgc agcgggcggc acggttgccg cctcggccgc
     3301 cgcaccaagg gtgccagccg cggccgtggc gagcgcgcgc ggcgcaggca accagcggcg
     3361 cagcatcgcg tgcagcgcct cgcgtgtgaa gggcttgccg agatgatcgt ccatgcctgc
     3421 cgcgaggcaa gcgctacgcc cttcgcgggt cacatcggcg gtga
//
LOCUS       DATLKL010000194         1797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_70084, whole genome shotgun sequence.
ACCESSION   DATLKL010000194 DATLKL010000000
VERSION     DATLKL010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70084"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VM713_02540"
     CDS             <1..1052
                     /locus_tag="VM713_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83172.1"
                     /translation="LAVINPTAQVYTSTGERVQSPQDRLKYVRSEYARAVALYSPDHP
                     DVKRLKREMEGLEAATATDAGDARDEGNNGRRHLEEAETQLASARQRYSADHPDVVRL
                     ERLVASLRTRALAEPAPLPAVSASSLGNPSEKPPSAAADNPPYIQIQAQREAAVSQIA
                     ALRKKRDELEGSLTGLERHLARTPMVERDYDAMLRDLDSAQIEYRQVRQKQNDAETAE
                     NLETERKGERFTLIEPPFTPEKPASPNRPLILIFGVMFALAAGFGTVALLEGTDATVR
                     GPEDLRVLLTSPPLATIPHIFTAIDRARMRRWRRNALLGGVASVIAALVLVHVLYRPL
                     DVLWVVALRRMGIEV"
     gene            1056..>1797
                     /locus_tag="VM713_02545"
     CDS             1056..>1797
                     /locus_tag="VM713_02545"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HVH83173.1"
                     /translation="MFVFPRGKLTDEAARLRAERASAGPLEADPPISLQELRPHGDRV
                     ATRVVEIDPLHLERERILPPGAAGVHGVHYKMLRTQVLLRLDKLAVNSLAVVGAATET
                     GKTLTAINLAIAIAADPERTALLIDLDLRRPRIHRRLGIEPAVGIEDCLRRSAPLPEA
                     LVRLGGYERFAVLPARERSLDSSELLSTRRTEEMIIEMRNRYRDRILVFDLPPVLQAD
                     DALAFARHAQAALIVVGEGRTRRVDVLRT"
BASE COUNT          318 a          617 c          593 g          269 t
ORIGIN      
        1 agctcgcggt aatcaacccc acggcacagg tctacacctc gaccggcgag cgcgtacagt
       61 ccccgcagga ccgcctgaag tacgttcgca gcgaatatgc gcgagcggtc gccctgtact
      121 cgcccgacca tccggacgtg aagcgactca agcgcgagat ggagggactc gaggctgcca
      181 ccgcgaccga tgcgggcgac gcccgcgacg aaggcaacaa cggtcgccgt cacctcgagg
      241 aagccgaaac gcagctagcg agcgctcgcc aacgctactc ggccgaccac ccggacgtgg
      301 tgcgtctcga gcggctcgtg gcatcgctga gaacgcgggc attggccgaa ccggcgccgc
      361 tgccggctgt gagtgcttct tcactgggca atccgtccga gaagccgccc agcgccgccg
      421 ccgacaaccc tccctacatt cagatccagg ctcaacgtga agccgccgtg agccagatag
      481 ccgcgctgcg caagaagcgc gatgagcttg agggttcgct cacagggctc gagaggcacc
      541 tggctcgcac tcccatggtc gagcgcgact acgacgcaat gctgcgcgat ctcgattcgg
      601 cgcagatcga atatcgccag gtgcgccaga aacagaacga cgccgagaca gcggagaatc
      661 tcgagaccga gcgcaagggc gagcgcttta cgctgatcga gccaccgttc acgcccgaga
      721 agccggcgag ccccaatcgg cccctgatcc tgatattcgg ggtcatgttc gcgctcgcgg
      781 cgggctttgg cacggtggcg ctactcgagg gcaccgacgc gactgtacga ggtcccgaag
      841 acctgcgcgt ccttctcacc tcgccaccgc tggccacgat tccgcacata ttcacggcga
      901 tcgaccgggc gcggatgcgc cgctggcgac ggaacgcgct gctcggcggg gtggcgagtg
      961 tcatcgcagc gctggtcctg gtgcatgtcc tttaccgccc gctcgatgtg ctgtgggtag
     1021 tggcactgcg tcgcatggga atcgaggtct agagaatgtt cgtattccca cgcggcaagt
     1081 tgaccgacga agcggcgagg ctaagagccg agcgcgcgag cgcggggccg ctcgaagcgg
     1141 accccccgat ctcgttgcag gagctgcgcc cgcacggcga ccgggtggcg acgcgcgtcg
     1201 tcgagatcga tccgctgcat ctcgagcgcg agcgcatcct gccgcccggg gcggcagggg
     1261 tccacggtgt gcactacaag atgctccgca cgcaagtgct gctgcgactc gacaagctcg
     1321 cagtcaactc gctggcggtg gtgggagctg ccacagaaac gggcaagacc ctcaccgcca
     1381 tcaacctcgc catcgcgata gccgccgacc ccgagcgcac cgcgctcctc atcgatctcg
     1441 acctgcgccg gccgcgtatt caccgccgac tcggcatcga gccggccgtg gggatcgaag
     1501 actgtctgcg ccgctccgcc ccgctgccgg aagccctggt gcggctggga ggctacgagc
     1561 ggttcgcggt acttcctgcc cgcgagcgct cgctggattc ctcggagctc ctctcaaccc
     1621 ggcgcacgga ggagatgatc atcgagatgc ggaaccgcta ccgggaccgc atcctggtgt
     1681 tcgatctgcc gccggtgctg caggcggacg atgcgctggc cttcgcccgt cacgcacagg
     1741 cggcgctgat cgtggtcggc gagggacgca cgcgacgcgt cgatgtgctg cgcacga
//
LOCUS       DATLKL010000195         1648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_193927, whole genome shotgun sequence.
ACCESSION   DATLKL010000195 DATLKL010000000
VERSION     DATLKL010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193927"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VM713_02550"
     CDS             <1..210
                     /locus_tag="VM713_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVH83174.1"
                     /translation="GSLFITRPTLVTYTAKRKDLLASARDLFKVVESGAVKIRVQQTY
                     PLKEAAQAHIDLAARKTTGSTILLP"
     gene            377..685
                     /locus_tag="VM713_02555"
     CDS             377..685
                     /locus_tag="VM713_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83175.1"
                     /translation="MTIPRLLLAASAIGVCLVALPDANATEVVSDGPVNFVFQNHTSK
                     TVTLYVNGTRRCTAPPRGQCDTGTPTGQLELLFTWEGGHKTDTAGTTHSHLTYVLTER
                     "
     gene            complement(775..>1648)
                     /gene="rho"
                     /locus_tag="VM713_02560"
     CDS             complement(775..>1648)
                     /gene="rho"
                     /locus_tag="VM713_02560"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HVH83176.1"
                     /translation="AKSNPAADGARRSAGVKETEVRDTTRKDYTVRVIDLICPLGKGQ
                     RALIVSPPRSGKTVMLQNIAHSIAANHPESYLIVLLIDERPEEVTDMQRSVKGEVISS
                     TFDEPAARHVQVAEMVIEKAKRLVEYKRDVVILLDSITRLARAYNTVVPSSGKVLTGG
                     VDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIILD
                     RKLSDKRVFPSIDITRSGTRKEELLVERDVLAKMWVLRRILMPMGMNDAMEFLLDKLR
                     HTRTNSEFFQSMNQ"
BASE COUNT          301 a          562 c          478 g          307 t
ORIGIN      
        1 ggctcgctct tcatcacccg acccacgctc gtgacctaca ccgcaaagcg caaggacctc
       61 ttggccagcg cgcgcgacct tttcaaggtg gtcgagagcg gcgccgtcaa gatccgcgtg
      121 cagcagacct atccgctcaa agaggcggcc caggcgcaca tcgatctcgc cgcgcggaag
      181 acgacgggat cgaccatcct gctgccctaa cggctgggct tagtcaggac accatcgggc
      241 ttcgccaaag ggcgcctcgc ggcgcccttt ttgccgtttg ccgacccaac ctcttgagat
      301 ttctttaacg agccgctgtt acgatcgcgg tcgatccatc ccgccaggaa atcacgccgc
      361 cacgacggag aagatcatga cgatcccgcg tcttcttctc gccgcaagcg ccatcggggt
      421 ttgccttgtc gcgcttccag acgcaaacgc caccgaagtg gtatccgacg gacccgtgaa
      481 cttcgtcttt cagaatcaca cgagcaagac tgtaacgctc tacgtcaatg gcactcggcg
      541 ctgcaccgcg ccgccacgcg gccagtgcga taccggcacg ccgactggcc agttggagct
      601 gctgttcacc tgggaaggcg gacataagac cgacacggcc ggcacgacgc actcgcacct
      661 tacctacgtg ctcaccgagc ggtagcactc acgacccgcg ccgcgatccc gcaaacaaga
      721 agaggcgccc cacggggcgc ctccctcgtc ttcgcaaccg agcgcggccg gtcgctactg
      781 attcatagac tggaagaatt ccgagttcgt ccgagtatgg cgcagtttat cgagcaggaa
      841 ctccatcgcg tcgttcatac ccatgggcat gaggatgcgg cgcagcaccc acatcttcgc
      901 gagcacgtcg cgctcgacca atagctcctc cttgcgcgtg cccgagcggg taatgtcgat
      961 cgaggggaag acgcgcttgt ccgagagctt gcggtcgagg atgatctccg agttgccggt
     1021 gcccttgaac tcctcgaaga tcacttcatc cattcgcgac ccggtgtcga tgagcgccgt
     1081 cgcgatgatg gtcagcgagc cgccctcctc gatgttgcgc gcggcaccga aaaagcgctt
     1141 cggccgctgc agcgcgttcg catcgacgcc gcccgtaagc accttgcccg aggacggcac
     1201 cacggtgttg taggcgcgcg cgagccgcgt gatcgagtcg agcaggatca ccacgtcgcg
     1261 cttgtactcg acgagccgct tcgccttctc gatcaccatc tcggcgacct gtacatgccg
     1321 cgccgccggt tcgtcgaagg tcgagctgat gacctctccc ttgaccgagc gctgcatgtc
     1381 ggtgacctcc tccggccgct cgtcgatcag cagcacgatg aggtacgact ccggatggtt
     1441 ggccgcaatc gagtgggcga tgttctgcag catcaccgtc ttgccgctgc gcggcggcga
     1501 cacgatcagc gcacgctgac ctttgccaag cgggcagatc aagtcgatca cgcgcaccgt
     1561 gtagtctttt ctggtcgtat cgcggacttc agtttctttg accccagccg atctacgggc
     1621 accgtcggcc gcagggttgc ttttcgcc
//
LOCUS       DATLKL010000196         2807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_24824, whole genome shotgun sequence.
ACCESSION   DATLKL010000196 DATLKL010000000
VERSION     DATLKL010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24824"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..636)
                     /locus_tag="VM713_02565"
     CDS             complement(46..636)
                     /locus_tag="VM713_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HVH83177.1"
                     /translation="MKVLHIDSSILGQGSATRGLTREIVARLKSAHPDAKVTHIDLVA
                     EELPHLSHKSLARSDEGEAARNALALEQFLGADVLVIGAPIYNFSIPSQLKAWIDRIT
                     VAGKTFRYTANGPEGLAGGKEVIVAMARGSISGADRKGEFGESYLKFLFAFLGITKLR
                     FVRAEGLAISPEHREASLSAARAAIPSATALERLAA"
     gene            754..1650
                     /locus_tag="VM713_02570"
     CDS             754..1650
                     /locus_tag="VM713_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVH83178.1"
                     /translation="MQPLEGFYYFTQVIDHGGFAKAARALGIPKSRLSRHVSSLEGRL
                     GVRLVNRSTRRFVVTDVGHEVYQHAAAMLAEADAALEVVEFARAKPCGTIRASCPIAL
                     AQSALAPILPAFLEAYPAIRLELHVSNRRVDVLAESFDVALRVRSKPSGEDGLVMRTF
                     RQVDEFLVASPAYLKGAAALTEPAQLKQHTTLDYAGEFGRPWELLGPQGERAAAEQQP
                     RIVCQDFVVLREAALAGLGVARLPESVVRDDLKSGALVRVLPAWHSPQGIVHVVFPTR
                     RGMLPAVRALIDFLAERLPATL"
     gene            1816..>2807
                     /locus_tag="VM713_02575"
     CDS             1816..>2807
                     /locus_tag="VM713_02575"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HVH83179.1"
                     /translation="MRIPRTSAPVRVAWLAFLALAALTSARADEGDPPGRVARLSYVE
                     GAVSLEPAGLSDWTAAERNRPLTTGDRLWTDQQSVAELDLGDGVLRLGGMTGFAFLNL
                     DDRFAQIQLSGGILLVRVWDTSSGQSYEIDTPQLAVQLSQPGMYRIEVDEPGSTTVVK
                     VSEGEAVALGAAGSIPIDAQQSMVFTGSASDTSLSYSAASLGAPDDLDNWSATRDRQA
                     QESPSQQYVADGTPGTYELDDAGRWQSTPEYGYVWTPAVVEAGWVPYRFGHWVWISPW
                     GWTWVDEAPWGYATFHYGRWVLLGGVWAWVPGPRPLRPVYAPALVAWTRGPGFTG"
BASE COUNT          436 a          915 c          973 g          483 t
ORIGIN      
        1 caatcgggct gccgtgtgct tctttcaagg tcgtgctcct gaagctcaag ccgccaaccg
       61 ctcgagcgcc gtcgcggaag ggatggccgc gcgcgcggcg ctgaggctcg cttcccgatg
      121 ttccggagag atcgcgagac cttcggcgcg cacgaagcgc aactttgtga tgccgagaaa
      181 cgcgaacagg aacttgagat acgactcgcc gaactcccct ttgcggtcgg cgcccgagat
      241 gctcccgcgc gccatcgcga cgatgacctc cttgccaccg gcgaggcctt cggggccgtt
      301 ggccgtgtag cggaaggtct tgcccgcgac cgtgatgcgg tcgatccacg ccttgagctg
      361 cgatgggatc gagaagttgt agatcggcgc gccgatcacg agcacatctg cgccaaggaa
      421 ttgctcgagc gcgagcgcgt tgcgtgcagc ctcgccctca tcgctgcgcg cgagcgactt
      481 atgcgaaagg tgtggcagct cctccgccac gaggtcgatg tgggtgacct tcgcgtctgg
      541 atgcgcgctc tttaggcggg cgacgatctc gcgggtcagt ccacgggtgg ccgacccctg
      601 gcccaggatg ctcgaatcaa tgtgaaggac tttcatggct aatctcccaa tgccctacgg
      661 gcttgatgga gacattagtc cttggaacaa tgcgtgagta tccaggcaaa atggaacaga
      721 ttgttctacc tgtagaccta attggagccc tgaatgcagc ctctcgaggg gttctactac
      781 ttcacccaag tcatcgacca cgggggcttc gccaaggccg ctcgcgcgct cgggatcccg
      841 aaatcgcgcc tctcgcgcca tgtctcctcc ctcgagggga ggctcggcgt gcgcctcgtg
      901 aaccgctcca ccaggcgctt cgtggtgacg gatgtcggcc acgaggtcta ccagcatgcg
      961 gccgcgatgc tggcggaagc ggacgccgcg ctcgaggtgg tggagttcgc ccgtgccaag
     1021 ccctgcggaa caatccgggc gagctgcccg atcgcgctcg cgcaatccgc gctcgcgcca
     1081 atcctgccgg ccttcctcga ggcctacccg gcgatccggc tcgagctgca tgtcagcaac
     1141 cgccgggtcg acgtgctcgc cgagagcttc gatgtggcgc tgcgcgtgcg gtcgaaaccc
     1201 tcgggcgagg acggcctcgt catgcgcacg ttccgccagg tggatgagtt cctggtggcg
     1261 agccccgcgt atctgaaggg cgccgctgcg cttacggagc ccgcccagct caagcagcac
     1321 acgacgctcg attacgcggg cgagttcggg cggccctggg agcttctcgg accgcaaggt
     1381 gagcgcgcgg cggctgagca gcagccccgc atcgtctgcc aagacttcgt ggtgctgcgc
     1441 gaggcggcgc tcgcgggatt aggggtggca cggctcccgg agagtgtcgt acgggacgat
     1501 ctgaagagcg gcgcgctggt gcgcgtgctg cctgcctggc acagccccca gggcatcgtg
     1561 cacgtcgtct tcccgacgcg gcgcgggatg ctgcccgcag tgcgggcgct catcgacttt
     1621 ctcgccgagc gcttgccggc gacgctctag gctgactagc cggcggggcc ggaccgcggt
     1681 aggccagatg gggcatccgc gcgttgaacg atcatggaag cttatcgagg gtggccggca
     1741 ggtaaggcga atggtgctgc gatgggcgaa gtcgatagtc ttagactagg gaaggccgca
     1801 gggagcaccg cacccatgag aatcccccgc acatccgcac ccgtgagagt cgcctggctc
     1861 gcgttcctgg cactcgccgc gctcaccagt gcgcgggccg atgagggcga tcctccgggc
     1921 cgcgtcgcgc ggttgagcta tgtcgagggc gcggtgtcgc tcgagcccgc gggcctttcg
     1981 gactggaccg ccgcggagcg caatcgcccg ctcaccactg gggaccggct ctggaccgac
     2041 cagcagtccg tagcggagct cgacctcggc gatggggtgc tgcgcttagg gggcatgacc
     2101 ggattcgcgt tcctgaacct cgacgaccgc ttcgcgcaga ttcagctgag cggcgggatc
     2161 cttctcgtgc gggtctggga cacctcgagc ggccagagct acgagatcga cacgccgcag
     2221 ctcgccgtgc agctctcgca gccgggcatg taccggatcg aggtcgacga gccgggcagt
     2281 acgacggtgg tcaaggtctc ggaaggcgag gcggtggccc tcggcgcggc cggttcgatt
     2341 ccgatcgacg cccagcagtc gatggtcttc acgggttcgg cctccgacac ctcgctctcc
     2401 tacagcgccg cctcgctggg tgcacccgac gatctcgaca actggtcagc aacacgcgat
     2461 cgccaggcac aggagtcgcc atcgcagcag tacgtcgccg atggcactcc gggaacctat
     2521 gagctcgatg acgccggacg ctggcagagc acccctgagt acggctacgt gtggaccccg
     2581 gctgtggtgg aggcgggctg ggtgccttac cgcttcggcc actgggtctg gatctcgccc
     2641 tggggttgga cctgggtgga cgaggcgcca tggggctacg ccacgttcca ctacggtcgc
     2701 tgggtgcttc tgggcggcgt ctgggcctgg gtaccggggc cgcgtcccct gcgaccggtg
     2761 tatgcgcccg cgctggtggc ctggacccgg ggaccaggct tcacggg
//
LOCUS       DATLKL010000197         3333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_148981, whole genome shotgun sequence.
ACCESSION   DATLKL010000197 DATLKL010000000
VERSION     DATLKL010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148981"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VM713_02580"
     CDS             complement(<1..332)
                     /locus_tag="VM713_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83180.1"
                     /translation="MAALKLALSVERLPFAAPFRIAGRVFEHQDAVVVTLEDGAHRGR
                     GEACGVFYLEDGVPQILAAIEAQRGAIERGIGRDALQGLLPPGGARNALDCALWELDA
                     QRAGKPVW"
     gene            complement(332..2404)
                     /locus_tag="VM713_02585"
     CDS             complement(332..2404)
                     /locus_tag="VM713_02585"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVH83181.1"
                     /translation="MNIKLPRASARTKIALLPAAAALLLTVAMALFAMGIAWSAPPSA
                     KAAAQSAPTATPAPAAPPAATAPAAPASPLTAADLDVWLDGYLPYALKTGDIAGAVIA
                     VVKDGAVVTERGYGFSDVGKRTPVDPQLTLFRPGSVSKLITWTAVMQLVEQGKIDLDA
                     DINKYLDFTVTGLGGKPITVRNLMQHTAGFEEHARGVISAKDDVGSFETLLKQAVPAR
                     IFAPGSTPAYSNYGCSLAGYIVERVSGEPFDVYLEKHSFEPLDMQHSSFRQPLPKELA
                     PLMSKGYRTASGEAEPFEYVGPRPAGSMSSTGDDMAHFMIAHLQDGEYHGNRILGAAT
                     AELMHNSPYGDPIGPLNRMELGFFETNINGREVIAHLGDTEWFHTSLHLFLKENTGLY
                     ASFNSPGKEGAVQTLRTELFQDFADRYFPAAVPHPVTAQVDAKTQAAHAALMSGAWQV
                     SRRADSNFLAALGLIGETKVGANKNGELQLPFPGPNGKPRHWVEIAPFVWQDVAGHDR
                     AAAKVVDGRPVRFSFDFLSPFMVFDRAPWYASSLWLTPLVCASLAALLLTALVWPVAA
                     IVRRRYQAPLALEPKALRAYLWSRIGALAIVAALGVWGLFMVLILNDTGTLGGRLDPL
                     LLLAQLLGIVAFLGGIGVMLWNLLAVWRGAGRRWPAKLWSVVLVVAAVAVLWVGLVAK
                     LMSVGTNY"
     gene            complement(2428..>3333)
                     /locus_tag="VM713_02590"
     CDS             complement(2428..>3333)
                     /locus_tag="VM713_02590"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HVH83182.1"
                     /translation="VGEWACAEATVSTGLPRLGPAAAHARGASGLVIGVANFGGVIGE
                     GWVPLLIEALEAGLDLVSGLHARLADVPRLAAAAARCGRRLIDVRIPPPGIPVATGRK
                     RPGRRLLTVGTDCALGKKYSALAITRAFAARGCAVDFRATGQTGILIAGGGMPMDAVV
                     ADFEAGAAEILSPDAEPDHWDVIEGQGSLFHPAYAGVSLGLLHGSQPDVIVVCHHPGR
                     ERALGYSDYPLPTVAETIDLNVRLGRRTNPAIRCGGVCLNTSLLESRAAHDLLVRESA
                     RLGLPVADPMRPGQEFDRLIDSCLA"
BASE COUNT          522 a         1152 c         1170 g          489 t
ORIGIN      
        1 ttccacaccg gcttaccagc gcgctgcgcg tcgagctccc acagcgcgca atcgagcgcg
       61 ttgcgcgccc cgccgggcgg cagcagtccc tgcagcgcgt cacgcccgat gccgcgctcg
      121 atcgcgccgc gctgcgcctc gatcgccgcc agtatctgcg ggacgccgtc ctcgagatag
      181 aagacgccgc aggcctcccc ccggccgcga tgcgcgccgt cctcgagcgt gacgaccacc
      241 gcatcctgat gctcgaacac ccgcccggcg atgcggaacg gcgccgcgaa cggcagtctc
      301 tcgaccgaca gtgcgagctt gagcgccgcc atcagtagtt agtcccgacg ctcatcagct
      361 tagctaccag gcccacccac agcaccgcga ccgcggcgac caccagcacc acgctccaga
      421 gtttcgcggg ccagcggcgg ccggcgccgc gccacacggc gaggaggttc cacagcatca
      481 ccccgatgcc gccgaggaaa gccacgatgc ccagcagctg cgcaaggagc agcaacggat
      541 cgaggcggcc gccgagggtt ccggtgtcgt tgaggatcag caccatgaag aggccccaca
      601 ccccgagggc cgcgacgatg gcgagcgcac cgatgcggct ccagaggtag gcgcgcagcg
      661 ccttgggttc gagcgccagc ggggcctggt agcgacggcg cacgatcgcc gcgaccggcc
      721 acacgagtgc ggtgagcagc agcgccgcga ggctcgcgca cacgagcggc gtcagccaca
      781 ggctgctcgc gtaccacggc gcccggtcga ataccatgaa gggcgagagg aaatcaaagc
      841 tgaagcgcac cggtctgcca tcgaccacct tggcggcggc gcggtcgtgg ccggcgacgt
      901 cctgccacac gaacggcgcg atctccaccc agtgtcgcgg cttgccgttt ggtcccggga
      961 agggcagctg cagctccccg ttcttgttgg cgcccacctt ggtttcgccg atgagcccga
     1021 gcgccgccag gaagttgctg tcggcgcgcc gcgagacctg ccaggcgccg ctcatgagcg
     1081 ccgcgtgcgc cgcctgtgtt ttcgcatcca cctgcgccgt caccgggtga ggcacggcgg
     1141 ccgggaagta gcggtcggcg aaatcctgga acagctcggt gcgcagcgtc tgcaccgcgc
     1201 cctccttgcc cggactgttg aaggaggcgt acaggccggt gttctccttg aggaacagat
     1261 ggagtgaggt atggaaccat tcggtgtcgc cgaggtgcgc gatcacctcg cggccgttga
     1321 tgttggtctc gaagaagccg agctccatgc ggttgagcgg cccgatcgga tcgccgtagg
     1381 ggctgttgtg catcagctcc gcggtcgcgg cgccgaggat ccggttgccg tgatattcgc
     1441 cgtcctgcag gtgcgcgatc atgaagtgcg ccatgtcgtc gccggtcgag gacatcgagc
     1501 ccgcgggacg cggacccacg tactcgaagg gctcggcctc gcccgaggcg gtgcggtagc
     1561 ccttcgacat gagcggggcg agctccttcg gcagcggctg ccggaagctc gagtgctgca
     1621 tgtcgagcgg ctcgaagctg tgcttctcga ggtagacgtc gaatggctcg cccgagacgc
     1681 gctcgacaat gtagcccgcg agcgagcaac catagttgga ataggcgggt gtcgatcccg
     1741 gcgcaaatat ccgcgccggc accgcctgct tgagcagcgt ctcgaagctc cccacgtcat
     1801 ccttggcgga gatgactccc cgggcgtgct cctcgaagcc ggccgtgtgt tgcatgaggt
     1861 tccgcacggt gatcggcttg ccgccgaggc ccgtcaccgt gaagtccagg tacttgttga
     1921 tgtcggcatc gagatcgatc ttgccctgct ccaccagctg catgaccgcg gtccaggtga
     1981 tgagcttcga caccgacccg gggcgaaaca gcgtgagctg cggatcgact ggcgtacgct
     2041 tcccgacgtc cgagaagccg tagccgcgct cggtgaccac cgcgccgtcc ttgacgacgg
     2101 cgatgaccgc cccggcgatg tcgccggtct tgagcgcgta tggcaggtag ccgtcgagcc
     2161 agacgtcgag gtctgcggcc gtgagcgggg atgccggtgc cgccggcgcc gttgctgccg
     2221 gcggcgctgc cggtgcaggc gtggccgtcg gcgcgctctg tgccgccgcc ttggcgctcg
     2281 gcggcgcgct ccaggcgatt cccatcgcga acagcgccat cgccacggtc agcaggaggg
     2341 cggcagccgc cgggagcagc gcgatcttgg tgcgtgctga ggcgcgcggg agtttgatgt
     2401 tcaaggcttg atccttctgg tcgagtgcta tgccagacat gagtcgatga gcctgtcgaa
     2461 ttcctgtccc ggacgcatgg ggtcggccac cgggaggccg agccgcgcgc tctcccgcac
     2521 caggagatca tgtgccgcgc gggattcgag cagcgaggtg ttgaggcaga cgccgccgca
     2581 gcggatcgcc gggttggtgc gccgtccgag ccgcacgttg agatcgatcg tctcggcgac
     2641 ggtcggcagc gggtagtcgg agtagccgag cgcccgctcg cgccccggat ggtggcagac
     2701 gacgatcacg tccggctgac tgccgtgcag gaggccgaga gagacgcccg cgtaggccgg
     2761 atgaaacagc gagccctgcc cttcgatcac atcccagtgg tccggctcgg catcgggact
     2821 caggatctcg gcggcaccgg cctcgaaatc cgcgaccacg gcgtccatgg gcatgccgcc
     2881 gccggcgatc aggattccgg tctggccggt cgcgcggaaa tcgaccgcgc acccgcgcgc
     2941 ggcgaaggcg cgcgtgatgg cgagcgcaga gtatttcttg ccgagcgcac agtcggtgcc
     3001 gacggtaagg agccgcctgc ccgggcgctt gcgcccggtg gcgaccggaa taccgggcgg
     3061 cggaatgcgc acgtcgatga gcctgcggcc gcagcgcgcc gcggccgcgg cgaggcgtgg
     3121 tacgtcggcg agtctcgcat gcaggccgct cacgagatca agccccgcct ccagcgcctc
     3181 gatgagcagc ggcacccagc cctcgccgat cacgccgccg aagttcgcga ccccgatgac
     3241 gagcccgctc gcgccccgtg catgagccgc ggcgggcccg aggcgcggca ggccggtcga
     3301 aaccgtggcc tcggcgcacg cccactcgcc gac
//
LOCUS       DATLKL010000198         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_70120, whole genome shotgun sequence.
ACCESSION   DATLKL010000198 DATLKL010000000
VERSION     DATLKL010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70120"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VM713_02595"
     CDS             <1..601
                     /locus_tag="VM713_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVH83183.1"
                     /translation="HWKHAQVLAARYPRIRVEPNALYVDEGQVITSAGSAAGLDMLLH
                     LVRRDFGAKIANQVAQRLVLAPHREGGQTQFVPRPVARDERGRLARLLDHIRAHPEAR
                     HTLKSLAARAAMSTRTLQREFRAATGQAPYEWIVGERIGRARELLEGTGLAQQTIAER
                     VGIGSAESLRHHFRRRMGTTPARYRARFARRELPPDRRP"
     gene            complement(598..1842)
                     /locus_tag="VM713_02600"
     CDS             complement(598..1842)
                     /locus_tag="VM713_02600"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HVH83184.1"
                     /translation="MRKYLACALTSLLLVASCGGGGSSFTTPPPPPMSGPNVATLIVD
                     SGPAGTVNEAFVSVTICQPGSTSNCQTITGIQVDTGSSGLRIISSVLNASLLAALPQQ
                     TVGGTTTPVVECAQFADGYTWGPVVTADVTVASEKASGIPIQVIGSPSFTQVPAACSS
                     PVPNAENTVATLFANGLIGVGVFLQDCGSFCATNVVAGTYYQCPTNGGACASIMESVN
                     LQVANPVGAVAADNNGVIVELPSVPANGAATATGTLVFGIGTASNNSLGSATPLATDL
                     STGTITVTFNGNTLSQSFLDSGSDAYIFTDSSLSACAANSVGSGFYCETMPASLSATI
                     KTVAGMQLAANFTVGDATSMFQIMPLPTALPQLGAPLTAPAGPPLPPTFDFGLPLFYG
                     RNVYTAIETRAAGGTTGPYFAY"
     gene            complement(1864..>2212)
                     /locus_tag="VM713_02605"
     CDS             complement(1864..>2212)
                     /locus_tag="VM713_02605"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HVH83185.1"
                     /translation="SLKGALTSFSTVKGYGVHEITTPAGVHVREYVASDGTVFALSWQ
                     GPVIPDLQQMLGAYYASYARAASTQHSPNHRHFKVEQPGLVVQSNGRMRAFYGRAWDP
                     ARLPPNFTAADIR"
BASE COUNT          370 a          696 c          788 g          358 t
ORIGIN      
        1 acactggaaa catgcgcaag tcctggccgc ccgctatccg cgcatccgcg tcgagcccaa
       61 tgcgctctac gtcgatgagg gacaggtcat cacctcggcg ggctcggccg ccgggctcga
      121 tatgctgctg catctggtgc gccgcgactt cggcgcgaag attgccaacc aggtggcgca
      181 gcgtctggtg cttgctcccc accgcgaggg cggtcagacg cagttcgtcc cgcggccggt
      241 ggcgcgcgat gagcgcggcc gtctcgcgcg ccttcttgac cacattcgcg cgcaccccga
      301 ggcgcgccat acgttgaagt cactggctgc ccgggccgcg atgagcaccc gcaccctgca
      361 gcgcgagttc cgcgccgcga cgggacaagc accctatgag tggatcgtcg gcgagcgcat
      421 cgggcgggcc cgggaactcc tcgagggcac gggactcgcg cagcagacga tcgccgagcg
      481 ggtcggaatc ggcagcgcgg aatcgctccg ccaccacttc cgccggcgca tggggacgac
      541 gccggcgcgc tatcgggcgc gcttcgcgcg ccgggagctg cctccggaca ggcgtcccta
      601 gtaggcgaag taaggtccgg tcgttccgcc ggcggcgcgt gtctcgatgg ccgtgtagac
      661 gttgcggccg tagaagagcg gcaggccaaa gtcgaaggtc ggcggcagcg gcggcccggc
      721 gggagccgtg aggggagccc cgagctgcgg aagcgccgtg ggcagcggca tgatctggaa
      781 catcgacgtc gcatcgccga cggtgaagtt ggcggcgagc tgcatgccgg cgacagtctt
      841 gatggtggcc gaaagactcg caggcatggt ctcgcaataa aagcctgagc cgaccgagtt
      901 tgccgcgcag gcgctcaaag agctgtcggt gaagatgtag gcatccgagc cgctgtcgag
      961 aaaactctgt gaaagcgtgt tgccgttgaa ggttaccgtg atcgtgccgg tgctgagatc
     1021 cgtcgcgaga ggcgtggcgc tgccgaggga gttgttgctc gcggttccaa tgccgaatac
     1081 gagcgtaccg gtggcggttg ctgcgccgtt ggccggtacg ctcggcagct cgacgatcac
     1141 gccgttgttg tcggccgcga cggctccgac gggatttgca acctgaaggt tgaccgactc
     1201 catgatgctg gcgcaggcgc cgccgttagt cgggcactgg tagtaggttc cggccaccac
     1261 gttggtggcg cagaagctgc cgcaatcctg caggaagacg ccgaccccga tcagtccgtt
     1321 cgcgaagagg gtcgcgaccg tgttctccgc gttgggaacg ggactcgagc aggcggccgg
     1381 aacctgggta aatgatggag agccgatgac ctggatcggg atgccgctcg ccttctcgct
     1441 cgcaaccgtg acatccgcag tcaccaccgg cccccaggtg tagccgtccg cgaactgcgc
     1501 gcactcgacc accggcgtcg tcgtacctcc gacagtctgc tgcggcaacg ccgcgaggag
     1561 ggacgcgttg aggaccgagg agatgatgcg tagtcccgag gagccggtgt cgacctggat
     1621 cccggtgatc gtctggcagt tgctggtgct gcccggctga cagatcgtga cgctgacgaa
     1681 ggcctcgttg acggttcccg caggtcccga gtccacgatg agcgtcgcga cgttgggacc
     1741 ggacatcggt gggggcggtg gcgtagtaaa gctcgagccg ccgccgccgc aggaggcgac
     1801 gaggagcagt gaagtgaggg cgcaggccag gtactttcgc atgccagtta ccttcgaggc
     1861 gcgtcagcgg atgtccgcgg ccgtgaaatt cggcggcagc cgggccggat cccaggctct
     1921 gccgtagaaa gcccgcatgc gcccgttcga ctggacgaca agccccggct gctcgacctt
     1981 aaaatgccgg tggttcggtg agtgctgcgt ggacgcggcc cgggcgtagc ttgcgtagta
     2041 ggcgccgagc atctgctgca ggtccggaat gaccggaccc tgccagctca gggcgaacac
     2101 cgtgccgtct gacgctacgt actcgcgtac atggacgccc gccggggtcg tgatctcatg
     2161 caccccgtaa cccttcaccg tactgaagga ggtgagagcc cccttgagcg ag
//
LOCUS       DATLKL010000199         2462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_24840, whole genome shotgun sequence.
ACCESSION   DATLKL010000199 DATLKL010000000
VERSION     DATLKL010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24840"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..880)
                     /locus_tag="VM713_02610"
     CDS             complement(14..880)
                     /locus_tag="VM713_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HVH83186.1"
                     /translation="MIPPSFDYHAPRSLGEAVALLASLGEDAKLLAGGHSLLPMMKLR
                     FAQPAHLIDLGAIQDLRGIREESGTLVIGAMTTEAELLGSALVRSKVPLLAEGASQIA
                     DPQVRYKGTIGGDLSHGDPGNDHPALMLALDAQFVLTGPKGRRTVPADGFFVGLYSTQ
                     LAGDEILTQIRIPIPAAGTGWSYQKLKRKTGDFATAAAAALLRMKGAAVAQVAIALTN
                     AAPTALRATAAERALVGKALDAASLAEAARLAMSICQPTADLRGDVDYKTAMCGEMTR
                     RALQAARARAGA"
     gene            1077..1619
                     /locus_tag="VM713_02615"
     CDS             1077..1619
                     /locus_tag="VM713_02615"
                     /inference="COORDINATES: protein motif:HMM:NF022605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2937 family protein"
                     /protein_id="HVH83187.1"
                     /translation="MSLVRGLLDRVVLVSAVVAGGLIPGFIAQYRQRLGGRLDQALLD
                     LAPWQKLADQSYRGDLQKLIEYHLASSDPTFHSEGNIIRALIATVHQLQSAVDALHGS
                     LFSQAGYLVLHADPTLTRATFADWVPTFALSGEGLAFAAVFALGAWLLFHALWWPVTL
                     SGRRLRNHGQPVLRRVEPTL"
     gene            complement(1638..>2462)
                     /locus_tag="VM713_02620"
     CDS             complement(1638..>2462)
                     /locus_tag="VM713_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVH83188.1"
                     /translation="GTGRRVFIPLIGTSDLKFELRPPAHLTEGRYFKPGLHEIVTTNA
                     CARQFEGFELGAKRQVRGADWAVVGHYDEGQSLQCVVLTDAETLMSLFSRNGYNRINV
                     RLNAPSDFTAFNRALEANPSLKLEAKTARAQIESDMKQFNGLLNFVSYFIGSIMAIGA
                     TFGAVNSLYSIVDARRRELATLRAIGFGSGAIVAATLVESILLAVPGALLGAALAWLL
                     FHRMAVSHFGFSFRLDVTPHLAELGIAWALVMGLIGGLLPALRAGRVPVTTALRAT"
BASE COUNT          386 a          846 c          820 g          410 t
ORIGIN      
        1 ctccttggag gctctaggcg ccggcgcgcg cgcgcgccgc ctgcagcgcg cggcgggtca
       61 tttccccgca catcgcggtc ttgtagtcga catcgccgcg cagatccgcc gtcggctgac
      121 agatgctcat cgcgagccgc gcggcctcgg cgaggctcgc ggcgtcgagc gccttgccca
      181 cgagcgcccg ctcggccgcc gtggcccgca gcgccgtcgg agctgcgttg gtgagtgcga
      241 tcgcgacctg cgccacggcc gcacccttca tcctgaggag cgccgcagcg gcggcggtcg
      301 cgaagtcgcc ggtcttgcgc ttgagcttct gatacgacca gccggtacct gccgccggga
      361 tgggaatgcg gatctgcgtc aggatctcat cgcccgccag ctgtgtggaa tacagcccca
      421 caaagaaccc gtccgccgga accgtgcgcc gccccttggg accggtgagt acgaattgag
      481 catcgagcgc cagcatcagc gccggatgat cgttgccggg atcgccgtgc gagaggtctc
      541 cgccgatcgt gcccttgtaa cgcacttgcg ggtcggcgat ctggctcgcg ccttcggcga
      601 gcagcggcac tttgctccgc accagcgccg agccgagcag ctccgcctcg gtggtcatcg
      661 cgccgatcac gagcgtcccg ctctcctcgc ggatgccgcg caggtcctgg atcgctccca
      721 ggtcgatgag gtgagcgggt tgcgcgaagc gcagcttcat catcggcagc aggctgtgcc
      781 cgcccgcgag gagcttcgcg tcctccccga gactcgcaag gagcgcgact gcttcgccga
      841 gggaacgcgg ggcatggtag tcgaatgacg gagggatcac ggcgtctcct taggcttgag
      901 tttcgcctcg ccaccaacgt ctgcgggcac gatgccgacc cgctcgagaa gagggctatc
      961 ttgctcgcgg cctcctccca aaatcaattc acggccggcg ctcgtgtgtc cccgtgtaac
     1021 actctgaaac ggcagcgaaa ccacgccctc gccgccgcca tgcaacaatg cgtgcaatgt
     1081 cgcttgtcag aggcctgctc gatcgcgtgg ttctggtgtc cgccgtggta gcgggcggcc
     1141 tcatccccgg cttcatcgcc caatatcgcc agcggctcgg tggacggctc gaccaggcgc
     1201 tgctcgacct ggcgccgtgg cagaagctcg cggaccagtc ctatcgcggc gacctccaga
     1261 agctgatcga gtaccacctc gccagcagcg atcctacctt tcactcggaa ggcaacatca
     1321 tccgcgccct gatcgccacc gtgcatcagc tgcagagcgc cgtggacgcg ctgcacggga
     1381 gcctcttcag ccaggccggc tacctcgtgc tgcacgccga tccgaccctg acccgtgcca
     1441 ccttcgccga ctgggtaccg acctttgccc tctcgggcga gggactcgcc tttgcggccg
     1501 tgttcgcgct cggcgcgtgg ctgctgtttc acgcgctctg gtggccggtg acgctcagcg
     1561 gccgccgtct cagaaaccat ggccaacccg tgctgcggag agtcgagccg actctttaga
     1621 ggggtcgccc gcgggcccta ggtcgcgcgc agtgccgtcg tcaccggtac gcgccccgcg
     1681 cgcagcgccg ggaggagccc gccgatcagg cccatgacaa gcgcccacgc gatgccgagc
     1741 tcggcaaggt gcggggtgac gtcgagtcga aagctgaagc cgaagtggct cactgccatg
     1801 cggtggaaga gcagccacgc gagcgcggcg ccgagcagcg cgccgggcac agccagcagg
     1861 atcgactcga cgagcgtcgc ggccacgatc gcccccgagc cgaagccgat tgcgcgcagc
     1921 gtcgcgagtt cccggcggcg cgcgtccacg atcgagtaga gcgaattcac cgcgccgaag
     1981 gtcgcgccga tcgccatgat cgagccgatg aagtagctca cgaagttgag gagcccgttg
     2041 aactgcttca tgtccgactc gatctgcgca cgcgccgttt tggcttcgag cttgagactt
     2101 ggattcgcct cgagggcgcg gttgaacgcg gtgaagtcgg agggtgcatt gagacgtacg
     2161 ttgatgcggt tgtagccgtt acgactgaat aggctcatga gagtctcggc gtcggtgagg
     2221 acgacgcact gcaggctctg accctcatcg tagtgcccga cgacggccca gtccgcgccg
     2281 cgcacctggc gcttggctcc gagctcgaat ccctcgaact gccgcgcgca ggcgttggtg
     2341 gtgacgatct cgtgaaggcc tggcttgaag tagcggcctt cggtgagatg cgccggcggc
     2401 cgcagctcga acttcagatc gctggtgcca atgagcggaa tgaagacgcg ccggccggtg
     2461 cc
//
LOCUS       DATLKL010000200         2757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_126447, whole genome shotgun sequence.
ACCESSION   DATLKL010000200 DATLKL010000000
VERSION     DATLKL010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126447"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /gene="phoR"
                     /locus_tag="VM713_02625"
     CDS             <1..1216
                     /gene="phoR"
                     /locus_tag="VM713_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HVH83189.1"
                     /translation="ALILHLAWVLTNLFRLEWWVRHRNYADPPNAGGVWGEIIAQIVR
                     LHRRKRFHKQRFVQLMRQLQRSTAALPNGVVILNAQRESVWFNRMAASLLNLRRTADL
                     GIRIENLLREPEFVRYLARQDFSNPVVIRQRTPEDCYLSLQVVPYGDGQLLLLVSDVS
                     RQMRLEAVRRDFVANASHELRSPLTVISGYLETLGQDPAFDPELLAPVAEMRRQAERM
                     TQIIRDLLELSRLEETDEVVGGAPIDVAALLSLLRKDVLARPVHPHEVRVRVESAAQL
                     IGDEPEIHSAFSNLVDNAAKYTPPEGSIEMRWWTDGEGGHLAVTDTGFGITSEHIPRL
                     TERFYRVDAGRSRATGGSGLGLAIVKHVLQRHGARLEVQSTLGEGSSFICHFPPERLA
                     QPVGAPLRTETA"
     gene            1332..2144
                     /locus_tag="VM713_02630"
     CDS             1332..2144
                     /locus_tag="VM713_02630"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HVH83190.1"
                     /translation="MANGNARRAGRTRVRTLFLSDIHLGYRHARAGELNEFLAGVEAE
                     SIVLVGDIVDALSLARRVFWSAEHTQVVRTLLARGRAGTRLVYIPGNHDESLGVFAEM
                     LQGQFEVHREWVHRTARGERLLVLHGDQFDGIVNCSPWLTRLGDALYELTVRVSDGVN
                     NLRRALGKAYWPLAERVKLSIPTSLRYIEKFERVAADHARTQGYDGVVCGHIHRANLR
                     HIEGTLYCNTGDWVESCSALIEAPGGELKLLRWPHAVRVPRRRSAGLVADAA"
     gene            2141..>2757
                     /locus_tag="VM713_02635"
     CDS             2141..>2757
                     /locus_tag="VM713_02635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVH83191.1"
                     /translation="MRIVLATDAWSPQTNGVVTTIKATADTLTQLGHTVRVLSPQNLA
                     SIPCPSYPEIRLAINPGAHVARELKAFRPHAIHIATEGTLGLAVRRYCRQRGVPFTTS
                     YHTRYPEYVAARWPIPVAVSYAWLRRFHGAARRTFVSSGTLERELAAQGFARLHLWRR
                     GVDLRRFRPREGSHPELAHLPRPIMAYVGRLAVEKNLDAFLGLDLP"
BASE COUNT          431 a          956 c          916 g          454 t
ORIGIN      
        1 tgcgctcatc ctgcatctcg cctgggtgct caccaatctg ttccggctcg aatggtgggt
       61 gcggcaccgc aactacgccg acccacccaa cgccggcggc gtatggggcg agatcatcgc
      121 gcagatcgtg cggctgcatc gccgcaagcg tttccacaag cagcgcttcg tgcagctcat
      181 gcgccagctg cagcgctcga ccgcagcgct ccccaacggc gtggtgatcc tcaacgcgca
      241 acgcgagagc gtgtggttca accgcatggc ggcgagcctg ctcaacctgc gccgcaccgc
      301 cgatctcggc atccgcatcg agaacctgct gcgcgagccc gaattcgtgc gctatctcgc
      361 gcggcaggac ttctcgaacc ccgtggtgat ccgtcagagg acccccgagg actgttacct
      421 gtcgctgcag gtcgtgcctt acggcgacgg gcagctgctg ctgctggtga gcgatgtgtc
      481 gcggcagatg cgcctcgagg cggtgcggcg cgacttcgtg gcgaacgcct cgcacgagct
      541 gcgctcaccc ctcaccgtga tttcgggtta tctcgagacg ctcggtcagg atccggcttt
      601 cgatcccgag ctcctcgcgc ccgtcgccga gatgcgccgc caggccgagc gcatgaccca
      661 gatcatccgc gacctgctcg agctctcgcg gctcgaggag accgacgagg tcgtgggcgg
      721 cgcgccgatc gacgtggcgg cgctcctctc gctcctgcgc aaggatgtgc tggcgcgccc
      781 cgtgcacccg cacgaagtgc gcgtgcgcgt cgagtccgcc gcgcagctga tcggggacga
      841 gcccgagatc cattcagcgt tctcgaacct ggtggacaac gcggccaagt acaccccgcc
      901 cgaaggctcg atcgagatgc gctggtggac cgatggggag ggcgggcatc tcgcggtcac
      961 cgatacgggc ttcggcatta cctcagagca cattccgcgt ctcaccgagc gcttttaccg
     1021 ggtcgatgcc ggacgctcgc gcgccacggg cggctcgggc ctcgggcttg cgatcgtcaa
     1081 gcacgtcctg cagcgccacg gcgctcgcct cgaggtgcag agcacgctcg gggaggggag
     1141 ctcgttcatc tgccactttc cccccgaacg gctggcgcag ccggtggggg cgcccctgag
     1201 gacggaaaca gcttaggttt actgtgcaac ccactaattc gtaagaaatc cttagtcatg
     1261 ctgtcatgca caagacacat ggaagtacga cccttggggg caccctgggg agtcttatgg
     1321 gtgagtcggg catggcaaac ggcaacgcga gacgcgcggg caggacgcgg gtacggacgc
     1381 tgttcctctc cgacatccat ctcggatatc ggcacgcacg cgctggtgag ctgaatgagt
     1441 tcctcgccgg agtcgaggcc gaaagcatcg ttctggtcgg ggacatcgtt gatgcgctga
     1501 gcctcgcccg ccgggtcttc tggtcggccg agcacacgca ggtggtgcgc acgctcctcg
     1561 cccgcggccg cgccggcacg cgcctcgtct acattcccgg caaccacgat gaaagcctcg
     1621 gggtgttcgc ggagatgctg cagggacaat tcgaggtgca tcgcgagtgg gtgcatcgca
     1681 ccgcgcgggg cgagcggctc ctggtgctgc acggagacca gttcgatggg atcgtcaact
     1741 gctcgccctg gctcacgcgc ctcggtgatg cgctgtatga gctcaccgtg cgcgtcagcg
     1801 acggggtcaa caacctgcgc cgggccctcg gcaaggccta ctggccgctg gccgagcggg
     1861 tgaagctcag cattccgacg agcctgcgct acatcgagaa gttcgagcgg gtggccgcgg
     1921 atcacgcccg cacccagggc tacgacggcg tggtgtgcgg tcatattcac cgcgccaacc
     1981 tccggcacat cgagggcacg ctctactgca acaccggcga ctgggtcgaa tcctgctccg
     2041 cgctcatcga agccccgggg ggagagctga agctcctgcg ctggccgcac gcggtccgtg
     2101 tgccgcggcg gcgcagtgcc ggcttggtcg ccgacgcggc gtgaggatcg tactcgcaac
     2161 ggatgcctgg tcgccgcaga ccaacggcgt cgtcaccacg atcaaggcga cggcagacac
     2221 cctgacgcag ctaggccaca cggtacgtgt cctgtcgccg cagaacctcg cgtcgattcc
     2281 ctgcccgagc tatcccgaga tccggctcgc catcaacccg ggggcgcacg tggcgcgtga
     2341 gctcaaggcg tttcggccgc acgcaatcca catcgcgacc gagggcacgc tggggctggc
     2401 ggtgcggcgc tactgccgcc agcgcggcgt ccctttcacc acctcctatc acacgcgcta
     2461 cccggaatat gttgcggccc gctggccgat ccccgtcgcg gtgagttacg cctggctgcg
     2521 gcgctttcac ggagcggcgc ggcgcacgtt tgtcagcagc gggacgctcg agcgcgagct
     2581 cgcggcgcag ggcttcgccc ggctgcacct gtggcgccgc ggcgtcgacc tgcggcgctt
     2641 ccggccgcgg gaaggatcgc accccgagct cgcccacctg ccgcgcccga tcatggccta
     2701 cgtcgggcgg ctcgccgtcg aaaagaatct cgacgccttc ctcggcctgg atctccc
//
LOCUS       DATLKL010000201         3147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_81301, whole genome shotgun sequence.
ACCESSION   DATLKL010000201 DATLKL010000000
VERSION     DATLKL010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81301"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /gene="gap"
                     /locus_tag="VM713_02640"
     CDS             complement(<1..932)
                     /gene="gap"
                     /locus_tag="VM713_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HVH83192.1"
                     /translation="MAIKVGINGYGRIGRNVLRALYEGRRTGELKIVALNDLGDPKTN
                     AHLTRYDTAHGRFPGEVKVDGDYMVVNGDRVRVLAERDPAKLPWGELGVEYVLESTGL
                     FTTKAKAGAHLKGGAKKVVISAPGGEDVDATIVYGVNDGTLKSGFTVISNASCTTNCL
                     APVAKVLNARFGIAAGIMTTIHAYTNDQVLTDVYHSDLRRARSATMSQIPTKTGAAAA
                     VGLVLPELKGKLDGFAVRVPTINVSLVDLTFSAKRATSVEEVNQAMKEAAAGPMKGIV
                     AYNSEPLVSVDFNHDAHSSVFDATLTKVIDGTLV"
     gene            complement(932..2929)
                     /gene="tkt"
                     /locus_tag="VM713_02645"
     CDS             complement(932..2929)
                     /gene="tkt"
                     /locus_tag="VM713_02645"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HVH83193.1"
                     /translation="MTTRRELANAIRALSMDAVQRANSGHPGMPMGMADIAQVLWGDF
                     LKCNPGNPAWVNRDRFVLSNGHGSMLLYSVLHLTGYPLTIADLEAFRQLGSRTPGHPE
                     YDLALGIETTTGPLGQGLANAVGMAFAEKLLALRFNRPNLPLVDHYTYAFCGDGCLME
                     GVSHEACSLAGTLGLGKLIVFYDDNGISIDGKVAGWFTDDTPGRFAAYGWHVVPGVDG
                     LDAEAVASAIRAARAEVSRPSLICCKTIIGYGAPHKQGTKEAHGEALGPEEVAAARVA
                     LGWPYPPFVVPEEIRAAWDRRAQGAALEAAWRELFKRYAQQYPDEAREFERRTRGELP
                     AGWRAAAAQSLAPLMKQAVAQATRASSQAVLSALAATLPELFGGSADLTQSNSTFFKG
                     ARTVTPQDETGDYLHYGVREFGMTAIMSGITLHGGLLPYGGTFLVFSDYARNAVRLAC
                     LMQLRVVLVYSHDSIGLGEDGPTHQPIEHLASLRALPHLSLWRPCDAAETAVAWTLAI
                     ERRGPTALVLTRQALAQQPRTAAQLQDIARGGYVLIDCQGTAEALVLATGSEIGIAAQ
                     AVNALNAAGRRVRLVSMPSTDTFDAQDEAYREQVLPRALARRLAVEAGATQCWWRYVG
                     SSGAVLGIDRFGASGKGADVFIHFGFTADNIAQHIRALLEA"
BASE COUNT          489 a         1051 c         1092 g          515 t
ORIGIN      
        1 ttgaccagcg tcccgtcgat caccttggtg agagtcgcgt cgaagaccga ggagtgcgcg
       61 tcgtggttga agtcgacgga caccagcggc tcggagttgt aagcgacgat tcccttcatg
      121 ggaccggccg ccgcctcctt catcgcctga ttgacctcct cgacggaggt ggcgcgcttc
      181 gcgctgaagg tgagatcgac cagcgagacg ttgatggtcg ggacacgcac cgcgaagccg
      241 tcgagcttgc ccttgagctc cggcagcacc agcccgaccg ccgcggcggc cccggtcttg
      301 gtggggatct gcgacatggt cgccgagcgc gcccgccgca ggtccgagtg atagacgtcg
      361 gtcagcacct ggtcgttggt gtaggcatga atggtcgtca tgatgccggc agcgatgcca
      421 aacctggcgt tgagcacctt ggcgaccggc gccagacagt tggtggtgca cgaggcattc
      481 gagatcacgg tgaagccgct cttcagcgtg ccgtcattga caccgtagac gatcgtggcg
      541 tcgacgtcct cgccccccgg tgcggagatc accaccttct tcgcgccgcc cttcagatgc
      601 gcgccggcct tggccttggt ggtgaagagc ccggtgctct cgagcacgta ttccacgcca
      661 agctcgcccc aggggagctt ggccggatcg cgctcggcca gcacccgcac tcggtcgccg
      721 ttcacgacca tgtagtcgcc gtccaccttg acttcgcccg ggaatctgcc gtgcgcggtg
      781 tcgtagcgcg tcaggtgtgc gttggtcttc gggtcgccca gatcgttgag cgccacgatc
      841 ttgagctcgc cggtgcgccg gccctcatag agtgcgcgca gcacgttgcg cccgatgcgc
      901 ccataaccgt tgatcccaac cttgattgcc atcaagcctc cagcagtgcg cgtatgtgtt
      961 gcgcaatatt atcggcggtg aagccgaagt gaatgaaaac gtcggcgccc ttgcccgaag
     1021 ctccgaagcg gtcgataccg agcaccgccc cgctgctgcc gacgtaacgc caccagcact
     1081 gggtggcgcc ggcctcgacc gcgaggcgcc gcgcgagcgc gcgcggcagc acctgctcgc
     1141 ggtaagcctc gtcctgggca tcgaaggtgt cggtggacgg cattgatacc agccgcacgc
     1201 gccgcccggc ggcattcagc gcattcaccg cctgcgccgc gatgccgatc tccgagcccg
     1261 tggcgagaac cagcgcttcc gctgtcccct ggcagtcgat cagcacatag ccgccgcgcg
     1321 cgatgtcctg tagctgcgcg gcggtgcgcg gctgctgcgc cagcgcctgc cgcgtcagca
     1381 ccagcgcggt ggggccgcgt cgttcgatgg ccagcgtcca ggcgaccgcg gtctccgccg
     1441 catcgcacgg ccgccacaga ctcaggtgcg gcagcgcccg caggctcgcg aggtgctcga
     1501 tgggctggtg cgtcgggcca tcctcaccga ggccgatcga gtcgtgtgag tacaccagca
     1561 ccacccgcag ctgcatcagg caggcgagcc gcacggcgtt gcgcgcgtaa tcggagaaca
     1621 ccaggaacgt gccgccgtag ggaaggagcc cgccgtgcag cgtgatgccg ctcatgattg
     1681 cggtcatgcc gaactcacgg acgccgtaat gcagatagtc gcccgtctca tcctgtggcg
     1741 tgacggtgcg cgcgcccttg aagaaggtgc tgttcgactg ggtgagatcg gccgagccgc
     1801 cgaacagctc cggcagcgtc gcggcgaggg cgctgagcac cgcctgcgag gaggcacgcg
     1861 tcgcctgtgc cacggcttgc ttcatcagcg gcgcgagtga ctgcgcggcg gcagcgcgcc
     1921 aacccgcggg cagttccccg cgcgtgcgac gctcgaactc gcgcgcctca tcggggtatt
     1981 gctgcgcata gcgtttgaac agctcgcgcc acgcggcctc gagcgccgcg ccctgcgcgc
     2041 ggcggtccca cgcggcgcgg atctcctccg gcacgacgaa gggcgggtac ggccagccga
     2101 gcgcgacccg cgcggcggcg acttcctcgg ggccgagcgc ctcgccgtgc gcctccttgg
     2161 tgccctgctt gtgcggcgcg ccgtagccga tgatggtctt gcagcagatg agggatggtc
     2221 gcgagacttc ggcgcgcgcg gcgcggatcg cgctcgccac cgcctcggca tcgagcccat
     2281 cgacgccggg aacgacgtgc cagccatagg cagcgaagcg gcccggcgtg tcgtcggtga
     2341 accagccggc gaccttgccg tctatggaga tgccgttgtc gtcgtagaag acgatcagct
     2401 tgcccaggcc cagcgttcct gccagcgagc acgcctcgtg cgacacgccc tccatgaggc
     2461 agccgtcgcc gcagaaggca taggtgtagt gatcgacgag cggaagattc ggccggttga
     2521 agcgcagtgc cagcagcttc tcggcgaacg ccatgccgac cgcgttcgca agtccctgcc
     2581 cgagcggtcc ggtggtggtc tcgatcccga gcgccaggtc gtattcgggg tgaccgggcg
     2641 tgcggctgcc gagctggcgg aaggcctcga ggtcggcgat ggtgagcgga tagccggtga
     2701 ggtgcagcac cgagtagagc agcatcgagc cgtggccgtt cgagagcacg aagcgatcgc
     2761 ggttcaccca ggcgggattg cccgggttgc acttgagaaa atcgccccac agcacctggg
     2821 caatatcggc catccccatc ggcatgcccg ggtggccgga attcgcgcgc tgtacagcgt
     2881 ccatcgagag tgcgcggatg gcattggcga gctcgcggcg agtggtcatg cggtgccttg
     2941 ggtggtttgg ggaggccgcg gtgcctggcg gccatacagg ggcgcgcatt ttcccaaagg
     3001 cgcgggcgat gctcaaatcg ctggcaccgg ggcggggcac gcggcagata ttgccgcgcg
     3061 ggtgggcacc cgcggccagc gaccggccga tgcgacttaa ttttcaccgc ttgcgcaatt
     3121 gcgacgggcg tgcatcacat ttctgag
//
LOCUS       DATLKL010000202         1663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_182959, whole genome shotgun sequence.
ACCESSION   DATLKL010000202 DATLKL010000000
VERSION     DATLKL010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182959"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..853)
                     /gene="istB"
                     /locus_tag="VM713_02650"
     CDS             complement(68..853)
                     /gene="istB"
                     /locus_tag="VM713_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HVH83194.1"
                     /translation="MSLDHESLARALTRLKLTAIRDQLDSLLDEAAKRELSLAEALAF
                     LVEREIARKDERRIEMAGKIAHFPTVRDLDGFDFAAQPSLDRRQIRELAACRWVAHGE
                     ALLLLGPPGVGKTHLAIALGRAAIREGYSVLFVTAPALVAALAKGHVEGRLEERLGFF
                     AKPKLLIIDELGYLPFETNAAHLFFQLVSRRYERGSVLITSNRAVGEWGSVFGDPVVA
                     TAILDRLLHRSHVITIRGDSYRLREKRRSGLIKAALGDAAAVA"
     gene            complement(850..>1663)
                     /gene="istA"
                     /locus_tag="VM713_02655"
     CDS             complement(850..>1663)
                     /gene="istA"
                     /locus_tag="VM713_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVH83195.1"
                     /translation="VATLGYSRRPFVRAFRHERQTAWFDGLEGAFRHFDGVPQEVVFD
                     NARALVDYHDAATREVRFNERLHAFARYWRFHPHACAPYRARTKGKDERGVGYVKRNA
                     IAGHDFISWAALEGHLDWWMREIADQRVHGTTGEVPLARFQRDEAGALAPLNGRPPFG
                     QMRELVRRVQADCAIAVDGNSYSVPWRLIGESVQVMVCGGQIRVSHAGQEVAVHAETA
                     GRRQRIVDAAHFHGVAGVAPPVGGALPASTAPPPALLRPLAEYERAAGGGWS"
BASE COUNT          274 a          604 c          546 g          239 t
ORIGIN      
        1 ctgccccgca ccttacagcc gggcggcggc gtgttgtatt ccgtcacgtg acgataatcc
       61 aagggactca cgcgaccgcc gccgcgtcgc cgagagcagc cttgatcagc ccggagcggc
      121 gcttctcgcg cagccgataa ctgtcgccgc ggatggtgat gacgtggctg cggtgcagca
      181 gccggtcgag gatggcggtc gccaccaccg ggtcgccgaa cacgctgccc cactcaccga
      241 ccgcgcggtt gctcgtgatt agtactgagc cgcgctcgta gcgccgcgac accagctgga
      301 agaacagatg cgcggcgttg gtctcgaacg gcagatagcc gagctcgtcg ataatcagca
      361 gcttgggctt ggcgaagaag ccgagccgct cctcgagccg cccctcgaca tgccccttcg
      421 ccagtgcagc aaccagcgcc ggcgccgtca cgaacagcac cgagtagcct tcgcggatcg
      481 cagcgcgacc aagggcaatg gccagatgcg tcttgccgac cccgggcggc cccaacagca
      541 gcaacgcctc gccatgcgcc acccagcggc aggcggcgag ctcacggatc tgccgccgat
      601 cgagcgaggg ctgggcggcg aagtcgaaac cgtcgaggtc gcgcacggtc gggaagtggg
      661 cgatcttgcc cgccatctcg atgcgccgct cgtccttgcg cgcaatctcg cgttcaacca
      721 gaaaggccag cgcctcggcc aggctcagct cgcgcttggc cgcttcgtcc agcagactgt
      781 cgagctggtc acggatcgcg gtcagcttga gccgggtcaa cgcgcgcgcc aggctctcgt
      841 gatcgaggct catgaccagc ctccgccagc ggcccgctcg tattctgcca gcggtcgcaa
      901 cagcgccggc ggcggcgcgg tcgacgctgg cagggcgccg ccgactggcg gcgcgacacc
      961 ggcgacgccg tgaaagtggg ccgcatcgac gatgcgctgg cggcgcccgg cggtctcggc
     1021 gtgcaccgct acctcctggc cggcgtgaga gacgcgaatc tgcccgccgc agaccatcac
     1081 ctgcacgctt tcaccgatca gccgccacgg cacgctgtaa ctgttgccgt cgaccgcaat
     1141 ggcgcaatcg gcctgcaccc gacgcaccag ctcgcgcatc tggccgaacg gcggccggcc
     1201 attgagcggc gccagcgccc ccgcctcgtc gcgctggaaa cgtgccagcg gcacttcgcc
     1261 ggtggtgcca tgcacccgtt ggtcggcgat ctcgcgcatc caccagtcga ggtgcccctc
     1321 cagcgccgcc cagctgatga agtcatggcc ggcaatggcg ttgcgtttga catagccgac
     1381 gccgcgctcg tccttgccct tggtgcgggc tcggtacggc gcgcaagcgt gcggatggaa
     1441 gcgccaatag cgggcaaagg cgtgcaggcg ttcgttgaaa cgcacctccc gggtggccgc
     1501 atcatggtaa tcgaccagcg cccgggcgtt gtcgaacacc acctcctgcg gcaccccgtc
     1561 aaagtgccgg aacgcgccct ccagcccgtc gaaccaggcc gtttgccgct cgtgccggaa
     1621 ggcccggaca aacggacgcc gcgagtagcc cagcgtcgcc aca
//
LOCUS       DATLKL010000203         1611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_92616, whole genome shotgun sequence.
ACCESSION   DATLKL010000203 DATLKL010000000
VERSION     DATLKL010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92616"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VM713_02660"
     CDS             complement(<1..686)
                     /locus_tag="VM713_02660"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVH83196.1"
                     /translation="MDLAPPLERLAANAVKEDGVTGIVVGVGIGGATPTIMAAGLANV
                     ERKTPMTPETDWKVASIAKSFFAALALDLEQEGWFSLDDKLRRFLPDVPNAGRVSLRE
                     LLNHTSGYDDYITDAFVASARDEPSRVWTPRELLVYAQPQRERFAPGSRYDYSNTNYL
                     LLGMALNAALGQSPAKEIRRRFLVPLGLRHTWFAAEESVPRDALARGYADLDDSGVKR
                     DATGEPWPLGG"
     gene            complement(761..>1611)
                     /locus_tag="VM713_02665"
     CDS             complement(761..>1611)
                     /locus_tag="VM713_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HVH83197.1"
                     /translation="QRAAVAIEKAMGRRVTALASLGGGCISQVFRATLDDGTELVAKL
                     GDGSAALETEAYMLGYLKAHSRLPVPAVRHAGPGLLLMDYVESGDPIDAAAEEHAADL
                     FAALHAIHGERFGHEQDTLIGPLAQPNPWSERWLDFFRDFRLLHMARQALAAGRISPV
                     LMTRIERFAARLGEFLDESARPALIHGDLWAGNILVRRGRIVGLVDPAIYYADPEIEL
                     AFATLFGSLGEPFFRRYNALSPLKSGFFEARRHIYNLYPLLVHTRLFGGHYAGAVERT
                     LARFGR"
BASE COUNT          273 a          523 c          578 g          237 t
ORIGIN      
        1 ccgccgagcg gccagggctc gcccgtcgcg tcgcgcttga cgcccgaatc gtcgaggtcg
       61 gcatagccgc gcgccagcgc atcgcgtggc accgactcct cggccgcgaa ccaggtatgg
      121 cgcaggccaa gaggtaccaa gaagcggcga cggatctcct tggcgggcga ctgaccgagc
      181 gctgcgttga gcgccatgcc gagcagcagg taattggtat tcgagtagtc gtagcggctg
      241 cccggcgcaa agcgctcgcg ctgcggctgg gcgtagacga gcagctcgcg cggcgtccag
      301 acacggctcg gctcgtcacg ggcgctggcg acgaaggcgt cggtgatgta gtcgtcgtag
      361 cctgaggtgt ggttgaggag ctcgcgcaag ctcacgcgcc ccgcgttggg tacgtcgggc
      421 agaaaacgcc gcagcttgtc gtcgaggctg aaccagccct cctgctccag gtcgagcgcc
      481 aaggcggcga agaagctttt ggcgatgctg gcaaccttcc agtccgtctc gggcgtcatg
      541 ggcgtcttcc gctccacatt ggcgagcccg gcggccatga tcgtcggggt cgcgccgccg
      601 attccgacgc ccaccacgat gccggtgacg ccgtcctcct tgaccgcgtt ggcagccagc
      661 cgctcgagcg gcggcgcaag gtccacggcg cggagcgggg ccgcgagaag gaccaggcca
      721 gccgcgaaca aggctcttgc gatcatcctc atcgatcggc tcatcgacca aagcgtgcaa
      781 gggtgcgctc gaccgccccc gcataatgtc ccccgaacag gcgggtgtgc acaagcagcg
      841 gatagaggtt gtagatgtga cgcctggcct cgaagaagcc ggatttgagc gggctcagcg
      901 cattgtagcg gcggaagaat ggctcgccca ggctgccgaa cagggtggcg aacgcgagct
      961 cgatctcggg gtcggcgtag tagatcgcgg gatcgaccag accgacgatg cggccgcggc
     1021 ggaccaggat gttgccggcc cagaggtcgc catgaatgag tgctggccgc gcggactcgt
     1081 cgagaaactc gccgagccgg gccgcgaagc gctcgattcg cgtcatgagc acgggcgaga
     1141 ttctcccggc cgcaagcgcc tggcgcgcca tgtggagcag gcggaaatcg cggaagaagt
     1201 cgagccaccg ctcgctccag ggattgggct gggccagcgg cccgatgagg gtgtcctgct
     1261 catggccgaa gcgttcgcca tgaatggcgt gaagggcagc gaagagatcg gctgcgtgct
     1321 cctccgcggc cgcatcgatc ggatcgcccg actcgacata gtccatgagc agcaagcccg
     1381 ggccggcatg acggacggcg ggaacgggca gccggctgtg cgccttgaga tagccgagca
     1441 tgtaggcctc ggtctccagc gccgcgctgc cgtcgccgag cttggccacg agttcggtcc
     1501 cgtcgtcgag ggtcgcgcgg aagacctggg aaatgcagcc gccgccgagg gaggcgagcg
     1561 ccgtcacccg gcgccccatc gctttctcga tcgccaccgc ggcccgctgt c
//
LOCUS       DATLKL010000204         3360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_81304, whole genome shotgun sequence.
ACCESSION   DATLKL010000204 DATLKL010000000
VERSION     DATLKL010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81304"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /gene="rarD"
                     /locus_tag="VM713_02670"
     CDS             <1..655
                     /gene="rarD"
                     /locus_tag="VM713_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HVH83198.1"
                     /translation="ISCNWLVYVWSVTHQHIVESSLGYYINPLVNVLLGVLVLRERPN
                     AVQWSAIGLAALAVLYLAVQAGRPPWIAFALALSFSLYGFVRKVISVDALPGLATETL
                     LLAPLALGYLAWCEATHVSAFSHDGPAIAALLMGSGIFTAVPLFLFAYGARRLPYSTV
                     GVLQYIGPSLQLACGVLFYDESFGPARAAGFGLLWAALILYAADGVWRARLRAAARV"
     gene            complement(684..1253)
                     /gene="efp"
                     /locus_tag="VM713_02675"
     CDS             complement(684..1253)
                     /gene="efp"
                     /locus_tag="VM713_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HVH83199.1"
                     /translation="MASYTTSEIRGGLKVLLDGDPYTVIENEFVKPGKGQAFNRIKVR
                     NLKTGRTIERTFKSGESLEAADVVDMDMQYLYQDGDFWHFMVPENFEQYTAGKSAVGD
                     NAQWLKDGVVCIVTLWNNVPLVVTPPPHIELKVVETDPGLRGDTATGGQKPAKLETGA
                     VVRVPLFINEGEVIRIDTRTGAYISRGKQ"
     gene            1448..>3360
                     /locus_tag="VM713_02680"
     CDS             1448..>3360
                     /locus_tag="VM713_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVH83200.1"
                     /translation="MIEQSAVPLIVLAATREPVEAINGMLRRAGEPAHCTWIPALRDL
                     GDALTQINPELLVAMTDDAQELEAVIAIRDQVTPTVPILALAQEADEAAIAHALMLGA
                     RDLTSLAHPDRLQAVMMRELESFRARRTLDAAQRAAQDARSQLDTVLERSNDAIIQVQ
                     EGIVTDANPAWLELFGVVDGIAGQPVMDLFEESSHPALRGALAACLQGRWSGEHPLRA
                     NALPADGSILPVEIALARGEHEGEPSVRLVVPSAPRAPALQAEPRAAEGPAVADPYGL
                     LPRRELLAALAGRLATRQSGGMRCLAVVKLDKFATLERVLGVTASEDVVSEFVRLLRE
                     TVRPKELSGRLGGVLFLVLLERGNENDINAWAEQLLARVQKHVMRIRDKAVSVTCSIG
                     LSTVSPGRAGVDAVVADALEACRKGSSRGGNQSVQSDAADTESRVMSYDKVWVKHIKA
                     ALMENRFRLVQQPIASLRGEDPGMFDVLVRMVDPQGKEVLPSEFMAAAARNDLMKNID
                     RWVVGASLSFAAQKKPECLFVRLSRETVKDDTFLDWLGNHLRSSRAEPERLCFQVTEE
                     SAATYVTQVKTLANALRERRFRFALESFGGGRDSLGMLDSVPLNFVKIDGAVIQGLAA
                     DSQLQQRVRALVEA"
BASE COUNT          472 a         1157 c         1165 g          566 t
ORIGIN      
        1 catcagctgc aactggctgg tgtacgtctg gagcgtcacc caccagcaca tcgtcgagtc
       61 gagcctcggc tactacatca atccgctggt caacgtgctg ctcggagtgc tggtgctgcg
      121 cgagcgcccg aacgccgtgc agtggagcgc gatcggcctc gcggcgctcg cggtgctcta
      181 tctcgccgtg caggccggcc gtccgccctg gatcgcgttc gccctggcgc tgagcttttc
      241 gctctacggt ttcgtgcgca aggtcatcag tgtcgacgcc ctgccggggc tcgcgaccga
      301 gactctgctg ctcgcgccgc tcgcactcgg ctatctcgcg tggtgtgaag cgacccacgt
      361 ctcggccttc agtcacgacg ggcccgcgat cgccgccctg ctcatgggca gcggcatctt
      421 cacggcggtg ccgctcttcc tcttcgccta cggggcgcgc cggctcccct actccaccgt
      481 cggcgtgctg cagtacatag gtccgagcct gcagctcgcc tgcggcgtgc tcttctacga
      541 tgagagcttc gggccggctc gggcggcggg ctttgggctc ctctgggcgg cgctgatcct
      601 ctacgccgcg gacggagtgt ggcgcgcgcg cctgcgcgcc gcggcgcgcg tctgacgcgg
      661 cgcgaccctc gcggcgctcg agcctactgc ttgccgcgcg agatgtacgc gccggtgcgc
      721 gtgtcgatgc ggatcacttc gccttcgttg atgaagagcg gcacccgcac cacggcgccc
      781 gtctcgagct tggcgggctt ttgaccgccg gtggcggtgt cgccgcgcag tcccgggtcg
      841 gtctcgacga ccttcagctc gatgtgcggc ggcggggtga ctacgagcgg tacgttgttc
      901 cacagcgtga cgatgcacac cacgccgtcc ttgagccatt gcgcgttgtc gccgaccgcg
      961 gacttcccgg ccgtgtactg ctcgaagttc tccgggacca tgaagtgcca gaagtccccg
     1021 tcctggtaga ggtactgcat gtccatgtcg acgacgtcgg cggcctcgag cgactcgccc
     1081 gacttgaagg tgcgctcgat ggtgcggccg gtcttgagat tgcgcacctt gatgcggttg
     1141 aaggcctggc ccttgccggg cttgacgaac tcgttctcga tcacggtgta cggatcaccg
     1201 tcgagcagga ctttgagacc gccgcggatc tcactggtgg tgtagctggc cattctcgga
     1261 tctcgtgatt tccacgggcg gcaggaccgg ccgccccgcc aaaaagggcc gcgtatgata
     1321 gcggcatcgc ctgggggccg ccagcgcgcc cgctggtgcc tttttccgcg aggtcgctca
     1381 cgcctggggc ctgaccccct gatgctagaa ttcgcccgcc cttgcgcgcg cattcacgga
     1441 ggccgtcttg atcgagcaga gcgccgttcc gctcatcgtg ctcgccgcca cgcgggaacc
     1501 cgtcgaggcg atcaacggca tgctgcggcg cgcgggggag ccggcccact gcacctggat
     1561 cccggcgctg cgcgatctcg gcgacgcgct cacccagatc aaccccgagc tgctggtggc
     1621 catgacggac gacgcgcagg agctcgaggc cgtcatcgcc atccgcgacc aggtgacccc
     1681 aacggtgccg atcctggcgc tcgcccagga ggccgacgag gccgccatcg ctcacgccct
     1741 catgctcggc gcccgcgacc tgacttccct cgcccacccc gaccgtctgc aggcggtgat
     1801 gatgcgcgag ctggagagtt ttcgtgcccg gcgcactctc gatgccgcgc agcgcgccgc
     1861 ccaggatgcc cgcagccagc tcgacacggt gctcgagcgc tcgaacgacg cgatcatcca
     1921 ggtgcaggag gggatcgtca ccgacgccaa ccccgcgtgg ctcgagctgt tcggcgtcgt
     1981 ggacgggatc gccggtcagc cggtgatgga tctgttcgag gagtcctcgc atccggcgct
     2041 gcgtggggcg ctcgccgcgt gtctgcaggg ccgctggagc ggagagcacc cgctgcgcgc
     2101 caatgccctg ccggccgacg gctccatcct gccggtggag atcgcgcttg ccaggggcga
     2161 gcacgagggc gagccgtcgg tgcggctggt ggtaccgtcg gcgccgcgcg ccccggcgct
     2221 gcaggccgag ccgcgcgccg ccgagggccc ggccgtcgcc gacccttacg gactgctgcc
     2281 gcgccgcgag ctgctcgcag ccctcgccgg gcggctcgcg actcgccagt cgggcggcat
     2341 gcgttgcctc gcggtcgtca agctcgacaa gttcgccacg ctcgagcgcg tcctcggcgt
     2401 gacggcgagc gaggacgtgg tgagcgagtt cgtgcgcttg ctgcgcgaga cggtgcggcc
     2461 gaaggagctg agcggacggc tcgggggagt cctcttcctg gtgctgctcg agcgcggcaa
     2521 cgagaacgac atcaacgcct gggccgagca gctgctcgcg cgggtgcaga agcacgtcat
     2581 gcgcattcgc gacaaggcgg tctcggtgac ctgctcgatc ggcctgtcga ccgtttcgcc
     2641 cggccgcgcg ggcgtcgatg cggtggtggc ggacgcgctc gaggcctgcc gcaaaggcag
     2701 cagccgcggc ggcaaccaga gcgtccagtc cgacgcggcc gacaccgaga gccgggtgat
     2761 gtcctatgac aaggtgtggg tcaagcacat caaagcggcg ctcatggaga accgcttccg
     2821 cctggtgcag cagccgatcg cgagcctgcg gggcgaggac ccgggcatgt tcgacgtgct
     2881 ggtgcgcatg gtcgatccac agggtaagga agtgctgccc tcggaattca tggcggcggc
     2941 ggcgcgcaac gacctgatga aaaacatcga ccgctgggtg gtgggagcgt cgctgtcgtt
     3001 tgccgcgcag aagaaacccg agtgcctgtt cgtgcgcctg tcgcgtgaaa ccgtcaagga
     3061 cgacaccttc ctcgactggc tcggcaacca cctgcgctcg agtcgcgccg agcccgagcg
     3121 gctgtgcttc caggtgacgg aggaaagcgc tgcaacctac gtcactcagg tgaagacgct
     3181 ggcgaacgcg ctgcgcgagc gccgcttccg cttcgccctc gagagcttcg gcggcgggcg
     3241 tgacagctta ggcatgctcg actcggtgcc cctcaacttc gtgaagatcg acggcgcggt
     3301 gatccaggga ctcgccgccg acagccaatt gcagcagcgc gtacgtgcgc tggtcgaagc
//
LOCUS       DATLKL010000205         1667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_13455, whole genome shotgun sequence.
ACCESSION   DATLKL010000205 DATLKL010000000
VERSION     DATLKL010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13455"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1667)
                     /locus_tag="VM713_02685"
     CDS             complement(<1..>1667)
                     /locus_tag="VM713_02685"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HVH83201.1"
                     /translation="LDAREVFRKIVHGAWKTGEPGVYFIDRANFYNPVPQLGSYEATN
                     PCGEQPLLPYDACNLGSINLGLFVRAGEVDWDRLRTVVQLCTHFLDNVIDANKYPLSE
                     IDSLAKRIRRIGLGVMGWADLLVRLGIPYNSEEAVGLGRRVMEFVDEEAKVASERLAE
                     QRGAFPEWPQSIWGPDATCARGPAGERIRPLRRLRNCNLTTVAPTGTISIIAGCSSGI
                     EPLFAVAFMRNQAGVLMPDVNEDFIAIARQEGWYSEGTMAQIAEAGHIRFAAVPERWQ
                     RVFVTAHDVTPEWHIQMQAAFQEFTDSAISKTCNFAHEATEEYVEKIYRYAYKLGCKG
                     ATVYRDGAREMQVLSTGSTAKKVQEQASAAGALAPGSPAAGQNALAEALGRLAELEAE
                     LGRTRAQLHEAEAENLQRRAKRSRPELLRGATRRIDTPLGTMYLNITEDDKGQPFEVF
                     ISLGKAGGALMADVEAIGRLISLALRSGIPMSEIHRQLRGISSDRVVGLGPHKVLSVP
                     DAIGIGIEKWMQEKQGIQQELLNTGGGSVGGSVPARQTLPTADGAAT"
BASE COUNT          246 a          581 c          555 g          285 t
ORIGIN      
        1 cgttgccgcc ccgtcggccg tggggagtgt ctggcgcgcc gggacactgc cccccacaga
       61 cccgcctccg gtgttcagga gctcctgttg gatcccctgc ttctcttgca tccacttctc
      121 gatgccgatg ccgatggcgt cgggcaccga cagcactttg tgcggtccca ggcccacgac
      181 gcggtcggag gaaatgccgc gcagctgccg atggatctcg ctcatgggga tgcccgagcg
      241 gagggcgagg gaaatcagcc ggccgatcgc ctcgacgtcc gccatgagcg cgccccccgc
      301 cttgccgagg gagatgaaca cctcaaaggg ttgacccttg tcgtcctccg tgatgttcaa
      361 gtacatcgtg ccgagtggcg tgtcgatccg ccgggtcgcg ccccgcagga gctcgggccg
      421 tgagcgcttg gcgcggcgct gcagattctc cgcctcggcc tcgtgcaact gggcgcgcgt
      481 ccgaccgagt tccgcctcga gctcggcgag ccgccccagc gcttcggcca gcgcgttctg
      541 gccggccgcc ggcgaccccg gcgcgagggc gccagcggcg ctcgcctgct cctggacctt
      601 tttcgccgtc gagcccgtcg acaagacttg catctcccgc gcgccgtcgc ggtagaccgt
      661 cgcgcccttg cagccgagct tataggcgta gcgatagatc ttctcgacgt actcctcggt
      721 cgcctcgtgg gcaaagttgc acgtcttgga gatcgccgag tcagtgaact cctgaaatgc
      781 cgcctgcatc tggatatgcc actccggggt tacgtcatgc gcggtgacaa agacgcgctg
      841 ccaccgctcc gggacggcgg cgaagcggat gtgcccggct tcggcgatct gcgccatcgt
      901 cccttccgag taccacccct cctggcgcgc gatcgcgatg aagtcctcgt tcacatcggg
      961 catcagcacg ccggcctggt tgcgcatgaa ggcgacggcg aagagcggct cgatgcccga
     1021 ggagcaaccg gcgatgatcg agatcgtccc cgtgggcgcg acggtcgtga ggttgcagtt
     1081 gcgcaggcgc cggagcgggc ggatccgctc cccggcgggg ccacgcgcgc acgtcgcgtc
     1141 ggggccccaa atcgactgcg gccactcggg gaacgcgccg cgctgctcgg cgagccgttc
     1201 ggaggccacc ttcgcctcct catcgacgaa ctccatcacg cgccgtccga gacccacggc
     1261 ctcctcggag ttgtacggga tgccgagccg gaccaggagg tccgcccagc ccatcactcc
     1321 gagcccgatg cggcggattc gcttcgcgag gctgtcgatc tcggagagcg gatacttgtt
     1381 cgcgtcgatg acgttgtcga ggaagtgcgt gcagagctgc accaccgtgc ggaggcggtc
     1441 ccaatccact tcgccggcgc gcacgaacaa cccgaggttg atcgagccca ggttgcaggc
     1501 gtcgtaggga agcaggggct gctccccgca cgggttcgtc gcttcgtagc tcccaagctg
     1561 tggcaccgga ttgtagaagt tcgcccggtc gatgaagtac acgccgggct ccccggtctt
     1621 ccaggccccg tggacgatct tgcggaacac ctcgcgggcg tcgagtt
//
LOCUS       DATLKL010000206         2252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_160252, whole genome shotgun sequence.
ACCESSION   DATLKL010000206 DATLKL010000000
VERSION     DATLKL010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160252"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..591)
                     /gene="queF"
                     /locus_tag="VM713_02690"
     CDS             complement(202..591)
                     /gene="queF"
                     /locus_tag="VM713_02690"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HVH83202.1"
                     /translation="MPATPTTQLETFVNPESERDYTIRMHIPEFTCLCPRTGQPDFAT
                     LDLEYVPDRRCVELKSLKLYIWSFRDRGAYHEAVTNEIADRLNAAVEARYLRLTARFN
                     VRGGIFTTVVAERRQPGWQPPPAVALP"
     gene            650..>2252
                     /locus_tag="VM713_02695"
     CDS             650..>2252
                     /locus_tag="VM713_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVH83203.1"
                     /translation="MRLTKIKLAGFKSFVDPTTISFPSNLTGVVGPNGCGKSNVIDAV
                     RWVMGELSAKTQRGDNMADVVFNGSSARKPIGSAAVELVFDNSDGKIGGPYASYSEIA
                     LKRQVSRDGQSAYYINGVRCRRKDITNLFLGTGLGSRSYAIIEQGMITRVIDAKSEDM
                     RAFVEEAAGISRYKERRKETEVRIAETRENLERLQDVRDEVEKQIRHLQRQAATARRY
                     QGLKDSERRLVAELIALKLRDLDSGAEVHDAELRGRELALQAELAGQRAAEAALEKQR
                     AAQTEHSERMSAVQARYYEAGADISRLEQTIEHTRELRERQRDDLAQASATLAELAAH
                     IGRDEQQLAAVRAELAQLAPALTAAEASERQAAARLESGEQALNDAQHRWEGHTLAAG
                     AAERTAQVERTRIGELEKQLRRLEALLTRLAEERVALASHGAGGQLSLLEQDAAQARA
                     ASEARSLALTRAQERVLLARTAQLAAEAALEAARTAREGARAELTSLEALQSAALSDR
                     AGEPVWRAGAGLAGQPRLAASLAVES"
BASE COUNT          373 a          771 c          802 g          306 t
ORIGIN      
        1 gaccttgcga accggtggcc ggccggacgc cttcctgccc ttggcggact tgggcgcagt
       61 tctcttggcc ggcatcaatc gctcctcgac agctcttcgg gctctttgaa gaacgcgcga
      121 ttataccggc cgaccctgag gtgtacagat gcacgccatc cggggcgatg gcgcagtgcg
      181 gcgggcggcg ggccgggatt ttcagggcag cgcgaccgcg ggcggcggct gccatcccgg
      241 ctgtcggcgc tcggcgacaa cggtggtgaa gatccccccg cgcacattga agcgcgccgt
      301 gaggcgcaga taccgtgcct cgaccgcggc gttcaggcga tcggcgatct cgttggtgac
      361 cgcctcgtga taggcgccgc ggtcgcggaa ggaccagatg tagagcttga gcgatttgag
      421 ctcgacgcaa cgccggtctg gtacgtactc gagatcgagc gtcgcgaagt ccggctgtcc
      481 ggtgcgcggg cacaggcagg tgaactccgg gatgtgcatg cggatggtgt agtcgcgctc
      541 gctttccggg ttgacgaaag tctcgagctg tgtggtgggc gtcgcaggca tattccacta
      601 cactacacca tccctggatg gccgggacgc gagggggttg aaggctcaga tgcggctgac
      661 caagatcaag ctcgccggct tcaaatcctt cgtcgatccc accaccatca gcttcccaag
      721 caatctcacc ggtgtcgttg gccccaacgg ctgcggcaag tcgaacgtca tcgacgccgt
      781 acgctgggtc atgggcgagc tgtccgccaa gacgcagcgc ggcgacaaca tggccgacgt
      841 ggtgttcaac ggctcgagcg cgcgcaagcc gatcggctcg gcagccgtcg agctggtgtt
      901 cgacaactcc gacggcaaga tcggcggccc gtacgccagc tacagcgaga tcgcgctcaa
      961 gcgccaggtc tcgcgggacg gccagtccgc ttactacatc aacggcgtgc gctgccggcg
     1021 aaaggacatc accaacctgt ttctgggcac cggcctgggc tcgcgcagct acgccatcat
     1081 cgagcagggc atgatcacgc gcgtcatcga tgccaagagc gaggacatgc gcgccttcgt
     1141 cgaggaggcg gccgggatct cgcgctacaa ggagcgacgc aaggaaaccg aggtgcgcat
     1201 cgccgagacg cgcgagaacc tcgagcggct gcaggacgtg cgcgatgagg tcgagaagca
     1261 gatccgccac ctccagcgcc aggcggccac ggcgcgccgc tatcagggtc tgaaggacag
     1321 tgagcggcgg ctggtcgccg agctcatcgc cctcaagttg cgcgacctcg acagcggcgc
     1381 cgaggttcac gacgcggagc tgcgcggccg cgagctcgcc ctgcaggccg agctcgccgg
     1441 gcagcgtgcc gccgaggcgg cgctcgagaa gcagcgtgcc gcgcaaacag agcacagcga
     1501 gcggatgtcc gcggtgcagg cgcgctacta cgaggccggc gccgacatct cccgcctgga
     1561 acagacgatc gagcacaccc gggagctgcg cgagcgacag cgcgacgacc tcgcccaggc
     1621 aagcgcgacg ctcgccgagc tcgccgccca catcggccgc gacgagcagc agctcgcggc
     1681 ggtgcgtgcc gagctcgcgc agctcgcgcc cgctttgacc gccgcggagg cgtccgaacg
     1741 ccaagccgca gcaaggctcg agtcgggtga gcaggcgctc aacgacgcgc agcaccgctg
     1801 ggaggggcat accctcgctg cgggtgctgc cgagcggacc gcgcaggtcg agcgcacccg
     1861 catcggcgag ctcgagaagc agttacggcg cctcgaggcc ctgctcacgc gtctcgccga
     1921 ggagcgcgtc gcgcttgcct cgcatggcgc cggtggccag ctgtcgctcc tcgagcagga
     1981 cgcggcgcag gcgcgcgccg cgagcgaggc gcgcagcctc gcgctcaccc gcgcccagga
     2041 gcgggtgctg ctcgcgcgca ccgcgcagct cgccgcggag gccgctctcg aggccgcgcg
     2101 cacggcgcgc gaaggcgcac gcgccgagct cacctcgctc gaagcgctgc agagcgcagc
     2161 gctctccgac cgcgccggag agccggtgtg gcgcgctggc gcgggactgg ccgggcagcc
     2221 gcggctcgca gcgagtctcg cggtcgagtc cg
//
LOCUS       DATLKL010000207         3087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_47444, whole genome shotgun sequence.
ACCESSION   DATLKL010000207 DATLKL010000000
VERSION     DATLKL010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47444"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VM713_02700"
     CDS             <1..693
                     /locus_tag="VM713_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HVH83204.1"
                     /translation="DMLGRAKKVVITKEDTTLIDGAGKKANIKARCNQIRAQIEETTS
                     DYDREKLQERLAKLAGGVAVIRVGGATEVEVKERKDRVEDAMHATRAAVEEGILPGGG
                     VALLYAAKSLDGLKGENSDQQHGIEIVRKALQIPARQIVENAGTDGSVVVGKLLEQKN
                     LNWGYDAQNMEYVDMFKAGIIDPTKVVRYALQDAASIGGLLVTTEAMVAEKPEKKPAP
                     PAPGAGMGDMDF"
     gene            1280..2328
                     /locus_tag="VM713_02705"
                     /pseudo
     CDS             1280..2328
                     /locus_tag="VM713_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            2500..3021
                     /locus_tag="VM713_02710"
     CDS             2500..3021
                     /locus_tag="VM713_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVH83205.1"
                     /translation="MRTTFTTVRPNTPLPALVEALQTAPFGLVFMVDDKGVLAGVISV
                     ADLAPVADSVILGGLPLVAADLAQPRPIVLEASDDPRRALRLMEARMGSHLPVVQDRA
                     SMRLVGVADVHELVLAYHKALLQARAEERGEVKGRRHTPRRHAAKRAPGPAPQPPGRD
                     SSTKAGVGRPKVA"
BASE COUNT          468 a          995 c         1068 g          556 t
ORIGIN      
        1 gacatgctcg gccgagcgaa gaaggtggtc attacgaagg aggacaccac ccttattgac
       61 ggcgccggca agaaggccaa catcaaggct cgctgcaacc agatccgcgc ccagattgag
      121 gagaccacct cggactacga ccgcgagaag ttgcaggagc ggcttgccaa gctggcgggc
      181 ggcgtcgcgg tcatccgggt gggcggcgcc accgaggtcg aggtgaagga gcgcaaggat
      241 cgggtcgagg acgccatgca tgcgacgcgc gccgcggtcg aggagggcat cctgccgggc
      301 ggcggggtgg cgctcctcta tgcagccaag tcgctcgacg ggctcaaagg cgagaactcg
      361 gaccagcagc acggcatcga gatcgtgcgc aaggcgcttc agatcccggc gcgccagatc
      421 gtggagaacg ccgggaccga cggttcggtc gtggtcggca agctcctgga gcagaagaac
      481 ctgaactggg gctatgacgc ccagaacatg gagtatgtcg acatgttcaa ggcgggcatc
      541 atcgacccga ccaaggtcgt gcgctatgcc ctgcaggacg cggcctcgat cggcggtctg
      601 ctggtcacca ccgaagccat ggtggccgag aagccggaga agaagccggc tccgcctgcg
      661 ccgggcgccg gcatgggcga catggacttc tgatcggagc cgagcgctcc gctttcggaa
      721 tctgcggggc cgtccttcgg gcggccccgc ctttattttt ggcgaagctc ggcgttgagc
      781 cgcctgggga aataggggcc ggcgcatggg cgggcggcgg gtaccggcgt aagccaaaac
      841 ggataccccc ttgatgccaa attgtcgcat gcgtctcgat ttcttggcat ggatcgagaa
      901 gcagcgttgc aatcatcgcc ccaatgttct taaacaggac tgcagttggg gatcggccgt
      961 cagcgaggaa ttttgggctc tggcacccag gtcaagggcg gcgccgcgca gccaaaggcg
     1021 cgggagcgcc gtacttaaca ttataaaagc gtcaagacgt taaggtgcaa ggacgccgtc
     1081 gggctggccg gccctggcgt cgacttcgcg tttaacggtt ggggcgatgc caagccgccg
     1141 tgtgctcctt cgcgggttta tccggcttcg ccgggtcatc gaggacgacc agcttctcct
     1201 ggtgctgatc gcggtcggga tcggcgcgct cggagccttg ggggtgatcg ccttccgggt
     1261 cctcatcacc gtcatccaga tggtggcgct cggcttcgac tcgccgcgcg tgctcgaata
     1321 tgcgcgcgac cttccctggt ggcggatcct gctcgcgccc accttgggcg gcctcgcgat
     1381 cgggctgttc gtgcgttacg tggtgcctgg cggccgctcc cagggcgtac ccgacgtgat
     1441 ggaggcggcg gcgctgcggg gtggccggct ctcgctcagg accacggctc tgggtgccgt
     1501 cgccaatgcc gcttcaatcg gggtaggggg ctcggtcggg cgcgaagggg cggtcatcca
     1561 tctgggtgcc accttcggct cctggatcgg acagcggatg cgcctgccgc cggccatgat
     1621 ccgcaccgcg cttgggtgcg gcgtggcctc agcggtcgcg gcctcgttca atgcgcccat
     1681 cgccggcgtg ttcttcgcgc tcgaggtcgt gatcgggact tacgcgatca gtccctttgc
     1741 cccgctggtc gtcgcctcgg tgatcggcac catcatcagc cggctctatt acggcgactt
     1801 cccgtcgttc atcctgccgc agtaccagat cgtcagcccg tgggagttct tcgctttcgc
     1861 gcttctgggc ttggtctgcg cggtggtcgc ggtcagcttc atggcctcga gcaacctcgt
     1921 gcagtacgtc gtcgagcggg tggacattcc gcgctggctg aggcccacgg ccggcggggc
     1981 cctgctgggg ctcatcgcgc tcgtcttccc gcacgtgctt ggggtcgggt acgaatcgac
     2041 caacgaggcc ctgttcggca ccatcccgct ttgggtcatg attgcgctgg tggtggcgaa
     2101 gaccgcggcc accgcgatct gcatcggcac cggcttcggc ggcggggatt tcagcgcctc
     2161 gctgttcctg ggagcaatga ccggcggcgc cttcggcttg gtcgcggccc acgccttccc
     2221 ggaccttgcc tcaagccacg gcgcctatgc gctggtcggt atggggccgt ggcgggtccg
     2281 gtgctgggag cgccgatcac caccgtcctg atcatctttg agatgaccgg cgactatgcc
     2341 atgacggtgg cagtgatgat cggtgtggtg atctccgcgc tggtcgtgca cgagaccttc
     2401 ggccgctcca tgtatgtcat gaagctcgag catcgcggcg tcaacctcaa gggcggccgg
     2461 gagatgagca tcttcagctc tatcccgatc gccgatctca tgcgcaccac cttcacgacc
     2521 gtgcggccga acacgcccct tccggccttg gtcgaggcgt tgcaaaccgc gcccttcggc
     2581 cttgtcttta tggtcgacga caagggcgtt cttgctgggg tcatcagcgt ggccgacctc
     2641 gccccggtcg ccgacagcgt gatcctgggc gggctgccgc tcgtcgccgc cgacttagct
     2701 cagccgcggc ccatcgtgct cgaggcgagt gatgacccgc gccgcgccct ccggctcatg
     2761 gaggcgcgga tgggcagcca tttgccggtc gtgcaggaca gggccagcat gcgcctggtc
     2821 ggtgtcgccg acgtgcatga gctcgtgctc gcctaccaca aggcactgct tcaggcgcgc
     2881 gccgaggagc gcggtgaggt caaggggcgc cgccacacgc cgaggcggca cgcggccaag
     2941 cgtgcgccgg gcccggcgcc gcaacccccc ggccgcgatt catcgacaaa ggccggcgtc
     3001 ggccgcccca aggtcgcttg aagccaagcc ccgtcccggc gtgaagccgg aactccttat
     3061 gggcgtggcc tagtcgagca ttctaca
//
LOCUS       DATLKL010000208         2985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_103860, whole genome shotgun sequence.
ACCESSION   DATLKL010000208 DATLKL010000000
VERSION     DATLKL010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103860"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /gene="dnaK"
                     /locus_tag="VM713_02715"
     CDS             <1..1378
                     /gene="dnaK"
                     /locus_tag="VM713_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HVH83206.1"
                     /translation="GGCTFDVSIIEIAEVDGERQFEVLSTNGDTFLGGEDFDLRIINF
                     LGEEFLKESGVDVRKDPLAMQRLKEAAEKGKIELSSTQQTDINLPYITADASGPKHLN
                     IKLTRAKLESLVEDLVQKTVAPCRTALKDAGVSTSDIAEVILVGGQTRMPLVQKYVKD
                     LFGKEPRKDVNPDEAVAVGAAIQAGVLAGEVKDVLLLDVTPLSLGIETLGGVMTRLIE
                     KNTTIPTKASNTFSTADDNQTAVTIHVLQGERDRAADNKSLGKFDLSDIPPAPRGMPQ
                     IEVSFDIDANGILNVSAKDKATGKEQKIVIKASSGLSEDEIKRMVRDAEAHAEEDKRF
                     RELVDTRNKADGLIHAVEKSLQTLGDKADAAERAKVESAVSDLRTALKGDDRNVIEKK
                     TEALAQASAAIAQRAYAEQQGGAAGPEAAAAGATGAGSGGGPGGAGKDDVVDAEFEEV
                     KDKGRKAS"
     gene            1405..2550
                     /gene="dnaJ"
                     /locus_tag="VM713_02720"
     CDS             1405..2550
                     /gene="dnaJ"
                     /locus_tag="VM713_02720"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HVH83207.1"
                     /translation="MPKRDYYKVLDVPRTATEAQIKQAYRRLAMKYHPDRNPNDRDAE
                     ERFKEAKEASEVLTDAHKRALYDQHGHAGIEAAARGAGRGGFGPGDAFSDIFGDVFGD
                     IFGGARRGGRSQVFRGADLRYELALDLDQAVFGTTAEIEVTKLAECDACHGSGAAKGS
                     QPATCESCGGAGQVRISQGFFQLQQTCPRCRGTGTVIRNPCDTCLGQGRVRRTRKLSV
                     KVPAGVDNEDRVRLSGEGEAGRNGGPPGDLYVEIHVREHPLFERDGEHLSCEVPVSFA
                     TAALGGSVSVPTLDGDVVLKVPAETQSGRVFRLRDKGVKPVRGGARGDLFCRVVVETP
                     VHLSAEQRELVRTLEESLKEHPGKHAPREKGFLEGVKRFFNSERGKA"
     gene            2567..>2985
                     /locus_tag="VM713_02725"
     CDS             2567..>2985
                     /locus_tag="VM713_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HVH83208.1"
                     /translation="MASDPRKAPLVRIAVIGVTGRMGLALLKAAPGFPQLLITGAIAS
                     PASLALGRDAGVLAGLGPTNLTVTSDLPQALSEADVAIDFSRAAATSANLGACRAARK
                     PLLIGTTAVDSALEPALAAAAGEIALLVAPNTSIAVAL"
BASE COUNT          517 a         1001 c         1049 g          418 t
ORIGIN      
        1 gggtggttgt accttcgacg tctccatcat cgagatcgcc gaggtcgatg gtgagcgcca
       61 gttcgaggtg ctctcgacga acggcgatac cttcctgggc ggtgaggact tcgatctgcg
      121 catcatcaat ttcctcggcg aggagttcct gaaggagtcg ggcgtcgacg tgcgcaagga
      181 cccgctcgcc atgcagcgcc tcaaggaagc cgcggagaag ggcaagatcg agctgtcctc
      241 gacccagcag accgatatca acctgccgta catcacggcg gatgcctcgg ggccgaagca
      301 cctcaacatc aagctgacgc gcgccaagct cgagtcgctg gtagaggacc tggtgcagaa
      361 gacggtggcc ccgtgccgca cggcgctcaa ggacgcgggc gtttctacct ctgatatcgc
      421 cgaggtgatc ctcgtcggcg gtcagacgcg catgccgctc gtgcagaagt acgtcaagga
      481 cctgttcggc aaggagccac gcaaggacgt caatcccgac gaggccgtgg ccgtgggagc
      541 cgcgatccag gcgggcgtgc tcgccggcga agtgaaggac gtgctgctgc tcgatgtgac
      601 gccgctgtcg ctcggcatcg agacgctcgg cggcgtgatg acgcgcctca tcgagaagaa
      661 caccacgatc ccgaccaagg cgtcgaatac cttctcgacg gccgacgaca atcagactgc
      721 cgtgaccatt cacgtgctgc agggcgagcg tgaccgcgcg gccgacaaca agtcgctcgg
      781 caagttcgac ctgtcggaca ttccgccggc gccgcgcggc atgccgcaga tcgaggtgtc
      841 cttcgacatc gacgccaacg gcatcctcaa cgtgtcggcc aaggacaagg ccaccggcaa
      901 ggagcagaag atcgtcatca aggcctcgag cggcctctcc gaggacgaga tcaagcgcat
      961 ggtgcgcgac gccgaggcgc acgccgagga ggacaagcgc ttccgcgagc tggtcgacac
     1021 gcgtaacaag gcggacggcc tgatccacgc cgtggagaag tcgctgcaga cgctcggcga
     1081 caaggccgat gcggccgagc gcgccaaggt ggagtcggcg gtatcggatc tgcgcaccgc
     1141 gctcaagggc gatgaccgta acgtcatcga gaagaagact gaggcgcttg cccaggcctc
     1201 ggcggcgatt gcccagcgcg cctacgccga gcagcagggt ggggcggcgg gacccgaggc
     1261 cgctgcggcg ggcgctacag gcgcgggcag cggcggcggc ccgggcggcg cgggcaagga
     1321 cgatgtggtg gatgcggagt tcgaagaggt gaaggacaag ggacgtaagg cgtcctaagg
     1381 cgcgccgccg gctacgcggc ccagatgccc aagcgcgact actacaaggt actggacgta
     1441 ccgcggacgg cgaccgaggc gcagatcaag caggcgtacc ggcgcctggc gatgaagtat
     1501 cacccggacc gcaacccgaa tgaccgggac gcggaagagc gcttcaagga ggccaaggag
     1561 gcctctgagg tgctcaccga tgcgcacaag cgcgcgctct acgaccagca cggtcacgcc
     1621 ggcatcgagg cagcggcacg cggcgccggc cgcggcggct tcggtcccgg ggatgccttc
     1681 agcgacatct tcggggatgt gttcggggac atcttcggcg gcgcacgccg cggcggccgc
     1741 tcgcaggttt tccgcggcgc ggatctgcgc tacgagctcg cgctcgatct cgatcaggcc
     1801 gtattcggca ccaccgccga gatcgaggtg accaagctcg ccgagtgcga cgcctgccat
     1861 ggctcgggcg ccgccaaggg cagccagccc gcgacctgcg aaagctgcgg gggcgcaggc
     1921 caggtgcgca tcagccaggg cttcttccag ctacagcaga cctgcccgcg ctgccgcggc
     1981 accggcaccg tcatccgcaa tccctgcgac acctgcctcg ggcaggggcg cgtgcgtcgc
     2041 acccgcaagc tctcggtgaa ggtccccgcc ggcgtcgaca acgaggatcg cgtgcgcctc
     2101 tccggcgagg gcgaggcggg ccgtaacggc gggccgcccg gcgatctcta cgtcgagatc
     2161 cacgtgcgcg agcatccgct cttcgagcgc gacggcgagc acctttcgtg cgaggtaccg
     2221 gtgagctttg cgaccgccgc cttgggcggc tcggtctccg tgccgacact cgatggtgac
     2281 gtggtcctca aggttccggc cgagacccag tcggggcgcg tcttccgcct ccgtgacaag
     2341 ggcgtgaaac cggtgcgggg cggtgcccgc ggcgatcttt tctgccgcgt ggtcgtcgag
     2401 acgccggtgc acctctcggc ggagcagcgc gagctggtcc gcacgctcga ggaatcgctg
     2461 aaggagcacc cgggcaagca cgcgccacgc gagaagggct ttctcgaggg tgtgaagcgc
     2521 ttcttcaact ccgagcgcgg caaggcgtag agggcgcgca cggcgtatgg caagcgatcc
     2581 acgcaaggcc ccgctcgtgc gcatcgcggt gatcggcgtg accggacgca tgggcctggc
     2641 gctgctcaag gccgctccgg gcttccctca gctcctcatc accggtgcca tcgcctcgcc
     2701 ggcgagtctc gcgctcgggc gcgacgccgg agtgctcgcg ggccttgggc ccacgaacct
     2761 cacggtcacg agcgatctgc cgcaggcgct cagcgaggcg gatgtcgcga tcgacttcag
     2821 ccgcgccgcc gccacgagcg ctaacctcgg cgcctgccgc gcggcgcgca agccgcttct
     2881 catcggcacc accgcggtcg actcagcgct cgagccggcc ctagccgccg cggccggtga
     2941 gatcgcgctg ctggtggcgc ccaacaccag catcgcggtg gcgct
//
LOCUS       DATLKL010000209         1548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_115159, whole genome shotgun sequence.
ACCESSION   DATLKL010000209 DATLKL010000000
VERSION     DATLKL010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115159"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VM713_02730"
     CDS             complement(<1..787)
                     /locus_tag="VM713_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HVH83209.1"
                     /translation="MAPLSRSSLIPLGMTLTLLAAGCSGSKGARYQGYVEGEFLYLAS
                     SQPGRLEHLAVTRGQQVERGTALFTLQAVEEKATQLQAQQQLAAAEAQLADLETGKRA
                     PEVAVVQAQLVQAQAAAQKSALQRERDEAQYRPGGVSREQLEASLAQARSDAAHVSEL
                     ESQLEVARLPGRGQQLKAQSRQVQAARAVLDQADWRVGEKSIAAPQAGIVYDTLYREG
                     EWVAAGNPVVQMLPPQNIKVRFFVPEKVLGSLSVGRRVSLHCDG"
     gene            complement(796..1455)
                     /locus_tag="VM713_02735"
     CDS             complement(796..1455)
                     /locus_tag="VM713_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVH83210.1"
                     /translation="MRTARATRRIGRPAQGTADAREQLLDAALTLFAERGVAGTTIAG
                     IAARAGVTPAMVHYYFSSRERLLDAVAEERLREIVTGVWSPVRQVTEIVPMLRGLVRR
                     ILTATELNPWLPSLWLREVISEGGQLRERLMRILPFDDVRHLIGTVASAQRRGEVSAA
                     LEPRLVLLSVLGLTFLPLATIRLWQVLPILQGVDREDIARHAEALLVGAFSKPRPARS
                     G"
BASE COUNT          239 a          537 c          540 g          232 t
ORIGIN      
        1 aaccgtcgca gtgcaggctc acccggcggc cgacggacag actcccgagg accttctcgg
       61 gaacgaagaa ccgcaccttg atgttctggg gcgggagcat ctgcaccacg ggatttccgg
      121 ccgcgaccca ctcaccctcc cgatagagcg tgtcgtagac gatgcccgcc tggggcgcgg
      181 cgatcgattt ttcgcccacc cgccagtccg cctggtccag cactgcgcgc gccgcctgca
      241 cctggcgcga ctgggccttg agctgctgcc cgcgccccgg caggcgggcc acttcgagct
      301 ggctctcgag ctcgctcacg tgagccgcat cgctgcgtgc ctgcgcgagg ctcgcctcga
      361 gctgctcgcg cgagacgccg ccggggcggt attgcgcttc gtcgcgttcg cgctgcagcg
      421 ccgacttctg cgccgcggcc tgcgcctgca cgagctgtgc ctgcacgacc gcgacctctg
      481 gtgcgcgctt gcccgtctcg aggtcggcga gctgcgcctc ggcggctgcc agctgctgct
      541 gcgcctggag ctgcgtggcc ttctcttcta cggcctgcag cgtgaaaagt gccgtgccgc
      601 gctcgacctg ctggccgcgg gtgacggcga ggtgctcgag ccgtccgggt tgcgacgacg
      661 ccaggtagag gaactccccc tcgacgtagc cctgatagcg cgcgcccttc gagcccgagc
      721 agccggcagc gaggagcgtg agcgtcatgc cgagcgggat gagggaagat cgggataacg
      781 gcgccatagg gctcctcagc cggacctcgc cggcctgggc ttcgagaacg cgccgaccag
      841 caacgcctcc gcgtggcgcg cgatgtcctc gcggtcgacg ccctggagta tcggcagcac
      901 ttgccagaga cggatcgtgg cgagcggcag aaaggtgagg ccgagtaccg aaagcaggac
      961 caggcgcggc tcgagcgccg cactgacctc accgcggcgt tgagcgctcg ccacggtgcc
     1021 gatcagatgc cggacgtcgt cgaagggcag aatgcgcatg agacgctcgc gcaactgccc
     1081 gccctcgctg atgacctcgc gcagccacag cgagggcagc caggggttga gctcggtggc
     1141 cgtgaggatg cgccgcacga ggccacgcag catcggcacg atctctgtga cttggcgcac
     1201 cggcgaccag acgccggtca cgatctcgcg gagccgctcc tccgcaaccg catcgagcaa
     1261 gcgctcgcgg ctgctgaagt agtagtggac catcgccgga gtgacgccgg ccctggcggc
     1321 gattccggcg atagtggtac cggccacgcc ccgctcggcg aacagcgtga gcgccgcgtc
     1381 gagcagctgc tcgcgcgcgt cggcggtgcc ctgcgcaggg cgcccgatgc ggcgtgtggc
     1441 cctggcggtg cgcatggtcc aataataatc aattaattaa ttaatttcaa aggcgaaccg
     1501 gggggtcgcc ccagacgccg ggcggctggc tagtcgacgc agtgaaag
//
LOCUS       DATLKL010000210         1671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_137871, whole genome shotgun sequence.
ACCESSION   DATLKL010000210 DATLKL010000000
VERSION     DATLKL010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137871"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VM713_02740"
     CDS             complement(<1..962)
                     /locus_tag="VM713_02740"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HVH83211.1"
                     /translation="MIVVVVESPAKAKTINKYLGKGYVVVPSYGHVRDLPAKDGSVRP
                     DEDFAMSWAVDRGAEKHIRAIASAAKKAKGVWLATDPDREGEAISWHVLELLKERRAV
                     DAAEVKRVVFNEITKSAVLDAMKHPREVNRELVEAYLARRALDYLVGFTLSPMLWRKL
                     PGSRSAGRVQSVALRLVCEREAEIEKFHPQEYWTIEVEFRNAETATLNARLTHLKGRK
                     LEKFDLGDQAAADAAVAALESGDYAVINVERKQQRRHPQPPFTTSTLQQEASRKLGFG
                     ASRTMRAAQRLYEGVDLNGDTVGLITYMRTDSVALANEAIVAGRR"
     gene            complement(1012..>1671)
                     /locus_tag="VM713_02745"
     CDS             complement(1012..>1671)
                     /locus_tag="VM713_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HVH83212.1"
                     /translation="GIDASAHDGALETGTVAVMAGGVDVVYPEENRPLYDSVAQRGLA
                     LSEMPMGTVPQGRHFPRRNRIISGLSLGIVVVEAAERSGSLITARLAGEQGREVFAVP
                     GSPLDARCKGTNGLIRDGAHLTESAADVLAVLSQAVQRTARGEARESAASAPAAPDAT
                     EPTAARRLVLESLGPSPVAVDEILRQSHLSPAAVSMVLLELELAGRIERHPGHRVSLL
                     S"
BASE COUNT          256 a          581 c          560 g          274 t
ORIGIN      
        1 cggcggcccg ccacgattgc ctcgttggcg agcgccacgc tgtccgtgcg catgtaagtg
       61 ataaggccga cggtgtcgcc gttgagatcg actccctcat acagccgctg cgccgcgcgc
      121 atggtgcggc tggcgccgaa gcctagcttg cgcgaggcct cctgctgaag ggtcgaggta
      181 gtgaatggcg gttgcggatg gcgccgctgc tgcttgcgct cgacattgat gaccgcgtaa
      241 tcgccgctct ccaaagcggc aacggcggca tccgccgccg cctggtcgcc gagatcgaat
      301 ttttcaagtt ttcggccctt gagatgggtc agacgcgcgt tgagcgtcgc agtctccgcg
      361 ttgcggaact ctacctcgat ggtccagtat tcttgcgggt ggaacttctc gatctcggct
      421 tcgcgctcgc ataccaggcg cagggccacc gactgcacgc gcccggccga gcggctgccc
      481 ggcagcttgc gccacagcat cggcgacagg gtgaagccga cgaggtagtc gagcgcgcgg
      541 cgcgcgaggt aggcttcgac cagctcgcgg ttgacctcgc gcggatgctt catcgcatcc
      601 aagacggcgg acttggtgat ctcgttgaag acgacgcgct tgacctcggc ggcgtcgacc
      661 gcccgccgct ccttgagcaa ctcgagcacg tgccacgaga tggcctcgcc ctcgcggtcc
      721 gggtcggttg cgagccagac gcccttggcc ttcttcgccg cactcgcgat cgcgcggatg
      781 tgcttctcag caccccggtc caccgcccag gacatggcga aatcctcgtc gggccggacc
      841 gagccgtcct tggccgggag gtcgcgcaca tggccgtagc tcggcaccac cacgtacccc
      901 ttgcccaggt acttgttgat ggtcttggcc ttggccggag attcgacgac gacaacaatc
      961 attccagcac taccaggtga tccgaggggg cgcgaggcta atgggttccg gctaggagag
     1021 caagctcacc cgatggccag gatggcgctc gatccggccc gccagctcga gttccagcag
     1081 caccatcgac accgccgcgg gcgacagatg ggactggcgc aggatttcgt caacggcgac
     1141 cggggacggc ccgaggctct ccagcacgag ccggcgcgcc gccgtgggtt cggttgcatc
     1201 gggcgcggcg ggggctgagg ccgcgctttc gcgcgcctcg ccgcgcgcgg tgcgctggac
     1261 cgcctgactt agaaccgcaa gcacgtcggc ggccgactcc gtgagatggg ccccgtcgcg
     1321 gatgaggccg ttcgtcccct tgcagcgcgc atcgagcggc gaccccggca cggcgaagac
     1381 ctcgcgcccc tgctcgccgg ccagtcgcgc ggtgatgagc gagcccgagc gctccgccgc
     1441 ctcgaccacc acgatcccga gcgacaggcc cgagatgata cgattgcggc gcgggaagtg
     1501 gcggccctgg ggcaccgtgc ccatcggcat ctccgacagc gcgagacctc gttgcgccac
     1561 ggagtcgtaa agcggccggt tctcctcggg atagaccacg tccacgccgc ccgccatgac
     1621 ggcgaccgtt ccagtttcga gcgcaccatc atgcgcgctc gcgtcgatcc c
//
LOCUS       DATLKL010000211         2289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_160306, whole genome shotgun sequence.
ACCESSION   DATLKL010000211 DATLKL010000000
VERSION     DATLKL010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160306"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VM713_02750"
     CDS             complement(<1..751)
                     /locus_tag="VM713_02750"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVH83213.1"
                     /translation="MSTAANVPSDADALRAVLTTLETVILGKPRQIRLCLACLLARGH
                     LLIEDVPGVGKTTLAHALAHVLGLAWQRVQFTSDLLPADIIGVSIFDRAHQRFDFRRG
                     PIFTQLLLADEVNRASPRTQSALLEAMEERQVSADGTTYRLPDVFFVVATQNPHEQLG
                     TFALPESQLDRFLMRVTLGYPDAAHERELLRGGERRDLLPEIPPALNPAGVLRVQREV
                     RAVYVADALLDYSQTLIACTRERSDLKLGLSP"
     gene            complement(748..>2289)
                     /locus_tag="VM713_02755"
     CDS             complement(748..>2289)
                     /locus_tag="VM713_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVH83214.1"
                     /translation="PAWYDGAGNVYVGRDETEVRARHGLDAQIPLRQDEDVLDTWFSS
                     ALWPFSTLGWPEETPALRRFYPGNVLVTGFDIIFFWVARMMMMGLKFMGDVPFRDVYI
                     TGLIRDEHGDKMSKSKGNVIDPLDIVDGISLDALVAKRTSGLMQPKLAGAIEKATRRQ
                     YPAGIAPHGTDALRFTFAALASPSRDLRFDLGRVGGYRNFCNKLWNAARFVTLAVGDS
                     APLGTATELSVADRWIRARFGRALATVEASLRDYRFDFAASALYEFTWYDFCDWYLEL
                     TKPVLQSQESPAAARDGTRRTLTGVLEALQRALHPFMPFITEEIWQRAAPLAGCAGET
                     VMLQPYPTAAEYPPDEAAERETSWIQAVVLGVRQIRGEMNINPARRIPLLLRDASAQD
                     REWAARHRPYLERLAGLDSVTVLATGAGAPPSAIALVGTLALLVPMAGLIDAPAEIER
                     LTKLIARAEKDLAVVRARLASERFIANAPAAVVAGDRERLAELERTTAGLNAQLERVR
                     GLEDS"
BASE COUNT          354 a          763 c          838 g          334 t
ORIGIN      
        1 gcggcgagag accgagcttc aggtcgctgc gctcgcgcgt gcaggcgatc agtgtctggc
       61 tgtaatcgag gagcgcgtcc gccacgtaca cggcgcgcac ctcgcgttgc acgcgcagca
      121 ctcccgcggg attgagcgcc ggcgggatct ccggcagcag gtcacgccgc tcgccaccgc
      181 gcagcagctc gcgctcgtgc gccgcatccg gatagccgag cgtcacacgc atcaggaagc
      241 ggtcgagctg cgactcgggc agggcgaagg tgccgagctg ctcgtgcggg ttctgggtcg
      301 cgaccacgaa gaacacgtcc ggcagccggt aggtggtgcc gtcggcgctc acctggcgct
      361 cctccatcgc ctcgagcaac gcactctgcg tgcgcggact cgcgcggttc acttcgtcgg
      421 cgagcaacag ctgcgtgaag atcgggccgc ggcggaagtc gaagcgctgg tgcgcgcgat
      481 cgaagatcga cacgccgatg atgtcggcgg gcagcaggtc gctggtgaac tgcacacgct
      541 gccacgcgag cccgagcacg tgggcgagcg cgtgcgcgag cgtggtcttg cccacgccgg
      601 gcacgtcctc gatcagcagg tggccgcgcg ccaggagaca ggcgaggcac agccggatct
      661 gccgcggctt gccgaggatg acggtctcga gcgtggtgag caccgcgcgc agggcgtcgg
      721 cgtcgctggg gacgttggct gccgtgctca cgagtcctcg agtccccgca cgcgctcgag
      781 ctgcgcattg agtcccgccg tggtgcgctc gagctcggcc aggcgctcgc ggtcgccggc
      841 taccaccgcc gcaggagcgt tggcgatgaa gcgttcgctc gcgaggcggg cgcgcacgac
      901 cgccagatcc ttttcggccc tggcgatcag tttggtgagc cgctcgatct ccgcgggggc
      961 atcgatgagg cccgccatgg gcacgagcag cgcgagcgtg ccgacgagcg caatcgccga
     1021 cggcggagcg ccggcgcccg ttgccagcac cgtcacgctg tcgaggcccg cgagacgctc
     1081 gaggtacgga cgatgccgcg cggcccattc gcgatcctgc gcgctggcat cgcgcagcag
     1141 cagcggaatg cgccgcgcgg gattgatatt catctcgccg cggatctgac gcacgccgag
     1201 caccaccgcc tgaatccagg aggtctcgcg ctcggctgcc tcgtcgggcg ggtactccgc
     1261 ggcggtcggg tagggctgca gcatgacggt ctcgcccgcg cagccggcga gcggcgcggc
     1321 gcgctgccag atttcctcgg tgatgaacgg catgaaggga tgcagcgcgc gctgcagggc
     1381 ctcgaggacg ccggtcaaag tgcggcgagt gccgtcgcgg gcggcggccg gggactcttg
     1441 cgactgcaac accggtttgg tgagctcgag gtaccagtcg caaaagtcgt accaggtgaa
     1501 ctcgtagagc gccgaggcgg cgaagtcgaa gcggtagtcg cgcagcgagg cctcgacggt
     1561 tgcgagcgcg cggccgaagc gggcgcggat ccaccggtcg gccaccgata gctcggtcgc
     1621 cgtgcccagc ggcgcggagt cgccgaccgc cagcgttacg aagcgcgccg cgttccacag
     1681 cttgttgcag aaattgcggt agccgcccac gcgccccagg tcgaagcgca ggtcgcggct
     1741 cggtgaggcg agcgcggcga aggtgaagcg cagcgcgtcg gtgccgtggg gcgcaatccc
     1801 ggccggatac tggcggcgcg tcgccttctc gatggcgccc gcaagcttcg gctgcatcag
     1861 gccgctggtg cgcttggcga ccagcgcgtc gagcgaaatg ccgtcgacga tgtcgagcgg
     1921 gtcgatcacg ttgcccttgg acttcgacat cttgtcgcca tgctcgtcgc ggatgaggcc
     1981 ggtgatgtag acgtcgcgga atggaacgtc ccccatgaac ttcagcccca tcatcatcat
     2041 gcgtgcgacc cagaagaaga tgatgtcgaa gccggtcacg agcacgttgc ccgggtagaa
     2101 ccgccgcagc gccggggtct cctcgggcca gccgagcgtc gagaacggcc acagggccga
     2161 tgagaaccag gtatcgagca cgtcctcgtc ctggcgcagc gggatttgcg cgtcgagccc
     2221 gtggcgggcg cgcacctcgg tttcgtcacg cccgacatac acgttgccgg cgccgtcgta
     2281 ccatgccgg
//
LOCUS       DATLKL010000212         1668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_205253, whole genome shotgun sequence.
ACCESSION   DATLKL010000212 DATLKL010000000
VERSION     DATLKL010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205253"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1668
                     /locus_tag="VM713_02760"
     CDS             <1..>1668
                     /locus_tag="VM713_02760"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83215.1"
                     /translation="IYKSTDAGETWSNAGLKDSERISKIVVDPRSSDTVYACVPGKLW
                     SDSADRGLYKTSDGGKTWRLILKGSNLSTGCGSVALQPGSPDVLLASLWDFRRKGWTF
                     RSGGDGPKATSGGGLYRSTDGGKSWTELTVAANKGLAAKPYGRIAIAYAPSEPQTVYA
                     FIESTDSALLISHDGGATWERGDKSTWMVWRPFYFANLIVDPKNPKRVFKTDGALILS
                     EDGGKSFTTVGGFNGAHGDVHDVFIDPANSEHVFNGDDGGLWTSYDGGNKWWKSDNLP
                     ISQFYHVSVDNADPYYVYGGLQDNGSWRGDSAYPGGITSARWVALFDGDGFWMYDDPS
                     DPDYVYAESQGGSIGRVNMRTFEGRDIQPKLGAEDLKLYKKLRFNWNTPIALSPHDPG
                     AVYIGSQFLYRSRDHGQSWQRISPDLTTNDPNEQKQEQSGGVTVDNSAAEMHTTIFSI
                     SESPLEKGMIWVGTDDGNLQLTRDDGAHWTNVVGNVRGLPKGSWVNWVQASSFEAATA
                     YAAFDRHTFGDMAPYVYVTHDFGKTWSPLVTPQVPKSVRGYAHVIKE"
BASE COUNT          318 a          579 c          528 g          243 t
ORIGIN      
        1 gcatctacaa atccaccgac gccggggaga cctggagcaa cgcgggccta aaggactccg
       61 agcgcatctc gaagatcgta gtcgacccgc gctcctcaga taccgtctac gcctgcgtgc
      121 ccggaaagct ctggagcgac tccgccgacc gcggcctcta caagacgagc gacggcggca
      181 agacctggcg cctgatcctc aagggctcga acctctcgac cggctgcggc agcgtcgcgc
      241 tgcagccggg cagccccgac gtgctgctcg cgagcctgtg ggacttccgt cgcaagggct
      301 ggacgttccg ttccgggggc gacggcccca aggcgacctc cggcggcggc ctgtaccgca
      361 gcacggacgg cggcaagagc tggaccgaac tcaccgtcgc ggccaacaag gggctcgcgg
      421 ccaagcccta cgggcgcatt gccatcgcct atgcgccgag cgagccgcag accgtgtacg
      481 ccttcatcga gtcgaccgac tcggcgctgc tcatctcgca cgacggcggc gccacctggg
      541 agcgcggcga caagagcacc tggatggtgt ggcgcccgtt ctacttcgcc aacctgatcg
      601 tcgacccgaa gaatcccaag cgcgtattca agaccgacgg cgcgctgatc ctcagcgagg
      661 acggcggcaa gagcttcacc accgtcggcg gcttcaatgg cgcacacggc gacgtgcacg
      721 atgtatttat cgaccccgcc aactcggagc acgtgttcaa cggcgacgac ggcgggctgt
      781 ggacctccta cgacggcggc aacaagtggt ggaagtcgga caatctgccg atctcgcagt
      841 tctaccacgt gagcgtcgac aacgccgatc cctactacgt ctacggcggg ctgcaggaca
      901 acggctcgtg gcggggcgac tcggcctacc ccggtggcat caccagcgcg cgctgggtgg
      961 cgctgttcga cggtgatggc ttctggatgt acgacgaccc ctccgatccc gactacgtgt
     1021 atgcggagtc ccagggcggc agcatcggtc gcgtcaacat gcgcacgttc gaggggcgcg
     1081 acatccagcc gaagctcggc gccgaggacc tcaagctcta caagaagctg cgcttcaact
     1141 ggaacacccc catcgcgctc tcgccgcacg acccgggagc cgtctacatc ggctcccagt
     1201 tcctctaccg ctcgcgcgat cacggccaga gctggcagcg tatctcgccg gacctcacca
     1261 ccaacgatcc caacgagcag aagcaggagc agtccggcgg cgtgacggtc gacaactcgg
     1321 ccgccgagat gcacaccacc atcttctcga tcagcgagtc gccgctcgag aaggggatga
     1381 tctgggtggg aaccgacgat ggcaacctgc aactcacgcg cgacgacggc gcgcactgga
     1441 ctaacgtggt cggcaacgtg cgcggcctgc cgaagggctc ctgggtgaac tgggtgcagg
     1501 cgagcagctt cgaagcagcg accgcctacg ccgccttcga ccgccatacc ttcggcgaca
     1561 tggccccgta cgtatacgtg acgcacgact tcggcaagac ctggtcgccg ctcgtgacgc
     1621 cgcaggtacc gaagagcgtc cgcggctacg cgcatgtcat caaggagg
//
LOCUS       DATLKL010000213         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_92679, whole genome shotgun sequence.
ACCESSION   DATLKL010000213 DATLKL010000000
VERSION     DATLKL010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92679"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2256
                     /locus_tag="VM713_02765"
     CDS             <1..2256
                     /locus_tag="VM713_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVH83216.1"
                     /translation="ERPSGSNAWLEATNPARLFHVRRDRDDDARRIARVLTGQSVGLV
                     LSGGGARGAAHVGVLKVLEQLHVPIDAIAGTSMGAVVGGLYASGLSARDIEKIMTSLN
                     WQAAFRDRPPREDLTLRRKQEDENFLVKFPLGVRGHRVVLPKGLIEGQGLSQMLRRLT
                     LPVARITDFDELPTRFRAVATDLESGEPAVLSSGDLTSAMRASMSAPGIFAPVERQGR
                     MLVDGGIADNVPVDVARAMGVDLVIVVDVGSPLQQRSKLTDVTAISNQMLAILLRRNA
                     EQQLATLTPRDILIAPALGDASSFDFGAVARYIGVGEAAARTAAAQLSSLGVSDEQMQ
                     HYEERRSAQRLPPPVIHFVQADGGSEEYSQSLDRLFKDMAGKPLDPGAVARRVTALYG
                     LGGLDTLDYRVVGHEDYYGLALDARPSSMGPNYLRFGLSLQDDFEGHATYDAAVRFVM
                     SDITRNAGEWVTDLQGGTTSLAATELFLPLAQFSGWFVTPHVSDVARALYILNGQSLE
                     AEYRLHTFQYGSDFGYQFSNWGEARVGAQRQQGHYVLTIGDPTDPNLPVQSLTAFNLI
                     NYFARFTYDRLDDINFPHSGQQATLQWSANRDVTGLGQYSDQVTASYVGAYSFGRDTF
                     SFSAAGGSTLQSNATDLNLQFQLGGFLNLSGLRANSLLGPNFGIARLLYYRQIGRGGP
                     GYFDVPTYLGASLEAGNVWQTRSAASFGNTQKDASVFLGMDTLLGPLYIATGFDQHGN
                     QAFYLFLGRTF"
     gene            complement(2285..>2505)
                     /locus_tag="VM713_02770"
     CDS             complement(2285..>2505)
                     /locus_tag="VM713_02770"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVH83217.1"
                     /translation="VQLVCYLQDLFTTEEARGQGIGRALIEEVATRARAAGAARVYWL
                     THETNQTARRLYDQVAANSGFIVYRRQL"
BASE COUNT          380 a          885 c          823 g          417 t
ORIGIN      
        1 gagcgtccct ccggctccaa cgcctggctg gaggcgacga acccggcgcg cctgttccac
       61 gtgcggcgcg accgggacga cgacgcgcga cgcatcgcgc gcgtcctcac cggccagtcg
      121 gtgggcctgg tgctctccgg cggcggcgcg cgcggcgccg cgcatgtcgg cgtgctcaag
      181 gttctcgagc agctgcacgt gccgatcgac gcgatcgccg gcaccagcat gggcgcggtg
      241 gtgggcggcc tgtacgcgag cgggctgtcc gcgcgcgaca tcgagaagat catgacctcg
      301 ctcaactggc aggccgcctt ccgcgaccgg ccgccgcgcg aggatctgac gctgcgccgt
      361 aagcaggagg acgagaactt tctggtgaag ttcccgctcg gagtgcgcgg ccaccgggtc
      421 gtgctgccga agggactcat cgaagggcag ggcctctcgc agatgctgcg gcggctgacg
      481 ctgccggtcg cgcgcatcac cgacttcgac gagctgccga cgcgctttcg cgccgtggcc
      541 accgacctcg agagcggtga gccggcggtc ctcagctcgg gcgacctgac gagcgcgatg
      601 cgcgccagca tgtccgcccc cggtattttt gctccggtcg agcgccaggg acggatgctg
      661 gtcgatggcg gcatcgccga caacgtcccg gtggacgtgg cgcgggcgat gggagtcgac
      721 ctcgtgatcg tcgtcgacgt cggctcgccg ctgcaacagc gcagcaagct taccgacgta
      781 accgccatct ccaaccagat gctcgccatc ctcctgcggc gcaatgccga gcagcagctc
      841 gccacgctca ccccgcggga cattctcatc gcgccggcac tcggggacgc ctcgtcgttc
      901 gatttcggag cggtggcgcg ctacatcggc gtcggcgagg ccgcggcccg caccgccgcc
      961 gcgcagctca gctctctcgg ggtcagcgac gagcagatgc agcactatga ggagcgccgt
     1021 tccgcgcagc gcctgccgcc gccggtgatc cacttcgtgc aggccgacgg cggctcggag
     1081 gagtattcgc agtcgctcga ccgcctgttc aaggacatgg ccggcaagcc cctcgacccg
     1141 ggcgcggtcg cgcggcgggt gacggcgctc tacggcctcg gcggtctcga caccctcgac
     1201 tatcgcgtcg tcggacacga ggactactac gggctcgcgc tcgatgcgcg tccgagctcc
     1261 atgggcccga actacctgcg cttcgggctg agtctccagg acgatttcga ggggcatgcc
     1321 acctacgacg ccgcggtgcg cttcgtcatg tccgacatca cccgcaacgc gggcgaatgg
     1381 gtcacggatc tgcagggcgg cacgacctcg ctcgccgcga cggagctgtt cctgccgctc
     1441 gcgcagttct ccggctggtt tgtgacgccg cacgtttcgg acgtggcgcg cgcgctctac
     1501 atcctcaatg ggcagagcct cgaggcggag taccggctgc atacattcca gtatgggtca
     1561 gatttcggct atcagttcag taactggggg gaggcgcgcg tcggcgcaca gcgccagcag
     1621 ggccactatg tgctgacgat cggcgatccg accgacccga atctgcccgt gcagagcctg
     1681 acagcgttca atctgatcaa ctacttcgcg cgcttcacct acgaccggct cgacgacatc
     1741 aacttcccgc acagcggcca gcaggcgact ctccagtgga gcgccaatcg cgacgtcacc
     1801 ggcctcgggc agtattcgga ccaggtaacg gccagctacg ttggcgccta ttcctttggc
     1861 cgtgacacct tctcattctc cgccgcgggc ggctcgacgc tgcagtcgaa cgccaccgac
     1921 ctcaacctgc agttccagct cgggggcttc ctgaatctct cgggactgcg cgcgaactca
     1981 ctgctcggtc ccaacttcgg catcgcgcgc ctcctctact atcggcagat cggccggggc
     2041 ggaccgggct acttcgacgt gccgacctac ctcggcgcct cgctcgaggc gggcaatgtc
     2101 tggcagaccc gcagcgcggc gagcttcggc aacacgcaaa aggatgccag cgtgtttctc
     2161 ggcatggaca cgctcctcgg gccgctctac atcgccaccg gcttcgatca gcacggcaac
     2221 caggcttttt acctgttcct tggccgcacg ttctgagggc ggcgctcccg cccgccgggc
     2281 aagcttacag ttgccggcgg tagacgatga atccagagtt cgcggcgacc tggtcgtaga
     2341 gccgccgcgc tgtctggttc gtctcgtgtg tcagccagta gacccgcgca gcgcccgcgg
     2401 cgcgggcgcg cgtcgcaact tcctcgatca gtgcccggcc gatgccctgt ccacgcgcct
     2461 cctcggtggt gaacagatcc tgcaggtagc acacgagttg gacgt
//
LOCUS       DATLKL010000214         2110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_205257, whole genome shotgun sequence.
ACCESSION   DATLKL010000214 DATLKL010000000
VERSION     DATLKL010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205257"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..878)
                     /locus_tag="VM713_02775"
     CDS             complement(285..878)
                     /locus_tag="VM713_02775"
                     /inference="COORDINATES: protein motif:HMM:NF017966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0017009 - protein-phycocyanobilin linkage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpcT/CpeT family chromophore lyase"
                     /protein_id="HVH83218.1"
                     /translation="MLGALAGVAALAGAAALAAPGCASPPKATEAELTVMLTMLPGRY
                     DNGAQADADVHSGTHPAHEAVALVIIHVYTPRLGHYVYYAQESAADEPRRVLSQRMYS
                     FQLDEKRGIVQTVYEFVDPLRWRDGLQSKDLFTAVQSEDVQPEGCQLLWTKNGDGFVA
                     AHDPKICPDSGGAAAAPQMELVAGALTVGDYKFRKGR"
     gene            complement(898..1932)
                     /locus_tag="VM713_02780"
     CDS             complement(898..1932)
                     /locus_tag="VM713_02780"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83219.1"
                     /translation="MKRLRTLVVVHASLVPPESLEGHSEKEIEEWRTEYDVTSNLRNL
                     GHEVRCIGVLDSLTELRSAIADWEPDIVFNLLEEFDGIVTYDQHVVAFLELMRQPYTG
                     CNPRGLLLSRDKTLAKQLLAFHRIATPQFAVFRRGARFHVPRKLRFPLFVKSTVEDAS
                     LGIAQASVVEDAPRLKERIAFVHDQLKSDALVEEYIEGRELYVGVMGNERLTRLPVWE
                     MVFGSMPDTLAAIATRKVKWDKRYQAKYGITTHAAADLPPAVLTELDRLSRRIYRALG
                     LSGYARMDFRVNAQGQVYVLEANANPNLEAAEDFAESARAAGTPYGELLERLMDLGLK
                     YRAQWRATYG"
     gene            complement(1929..>2110)
                     /locus_tag="VM713_02785"
     CDS             complement(1929..>2110)
                     /locus_tag="VM713_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83220.1"
                     /translation="ALAGERYSVDQVLHMLIERSAALRLYVRGNRRDALRYSRWMLER
                     LTAVYSQGETPHLSL"
BASE COUNT          345 a          716 c          683 g          366 t
ORIGIN      
        1 ctgcggttgg ggatcacgcc cacgaagcgc acctcaccat cgggctccgc caccgccacc
       61 gcaatcgtct cggcgtggac atccaaaccc acaaatcgta cgttcttcat cgccggctcc
      121 catttgtatg cggctctgcg ccgcgggtct tacccgatcc aaagcgtaat ccgcgctttg
      181 caaatcggga gccggtcgtt ccattgtgac tacctattgc ggggttgagt cacttttggg
      241 gtatcacata cgctcagccg ggcttgcaaa tcgcgctagc ccggtcagcg ccccttgcgg
      301 aacttgtaat ccccgaccgt cagcgcaccg gccaccagct ccatctgtgg cgccgccgcg
      361 gcgccgccgc tgtcgggaca gatcttcggg tcgtgggcgg cgacgaagcc gtcgccgttc
      421 ttcgtccaga gcagctggca gccctcgggc tgcacgtcct cgctctgcac ggcggtgaag
      481 agatccttgc tctgaaggcc gtcgcgccag cgcagcgggt caacgaactc gtacacggtc
      541 tggacgatgc cgcgcttctc gtcgagctgg aagctgtaca tcctctggct gaggacgcgc
      601 cgcggctcgt cggccgccga ctcctgcgcg tagtagacgt agtgcccaag gcgcggcgta
      661 tagacgtgga tgatgacgag cgccaccgcc tcgtgcgccg ggtgcgtgcc gctgtgcacg
      721 tccgcatcgg cctgcgcgcc gttgtcgtag cggcccggca gcatggtgag catcaccgtc
      781 agctcggcct cggtggcttt cggtgggctc gcgcagccgg gagcggcgag cgccgcggcg
      841 cccgcaagcg cggcaacgcc ggccagggcg ccgagcattc gggctgcgcc cgcgccctta
      901 cccatacgtc gcccgccact gcgcgcgata tttgagtccc aggtccatta ggcgttcgag
      961 cagctcaccg taaggagtgc cggcggcacg cgccgactcg gcgaagtcct cggcggcctc
     1021 gagattcgga ttggcgttgg cctcgagcac gtacacctgt ccctgcgcgt tgacccggaa
     1081 gtccatgcga gcatagccgg acaggccaag cgcccgatag atacgccgcg acaaccggtc
     1141 gagctcggtc aataccgccg gcggcagatc ggcggcggcg tgcgtggtaa tgccgtactt
     1201 tgcctggtag cgcttgtccc acttcacctt gcgcgtggcg atggcagcga gcgtgtccgg
     1261 catcgagccg aacaccatct cccacaccgg caggcgcgtc agtcgctcgt tgcccatcac
     1321 cccgacgtac agctcgcggc cctcgatgta ctcctcgacc agggcgtcgg acttgagctg
     1381 atcgtggacg aaggcgatgc gctccttgag ccgcggcgcg tcctccacca ccgaggcctg
     1441 ggcaatgccg agcgaggcgt cctcgaccgt ggacttcacg aacagcggaa aacggagctt
     1501 acgcggcaca tggaagcgcg cgccgcggcg gaacaccgcg aactgcggcg tggcgatgcg
     1561 atggaacgcg agcagctgct tggcgagtgt cttgtcgcgt gacagcagca gcccccgggg
     1621 gttgcagccc gtatagggct ggcgcatcag ctcgagaaag gccactacgt gctggtcgta
     1681 ggtgacgatg ccgtcgaact cctccagcag gttgaagacg atatccggct cccagtcggc
     1741 gatcgcactc cgcaactccg tgaggctgtc gaggacgccg atgcagcgca cttcatgacc
     1801 gagattgcgc aggttactgg tgacgtcgta ctcggtgcgc cactcctcga tttccttctc
     1861 gctgtggcct tcgagactct ccggcggcac caggctcgcg tgcaccacta cgagggtgcg
     1921 cagccgcttc ataaggacaa gtgaggagtc tcgccctggg agtacaccgc cgtgagccgt
     1981 tcgagcatcc agcgcgagta gcgcagggca tcgcgacgat tgccgcgcac gtacagccgg
     2041 agtgcggcgc tgcgctcgat gagcatgtgc agtacctgat cgacgctgta gcgctcgccc
     2101 gcaagcgccc
//
LOCUS       DATLKL010000215         1692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_205258, whole genome shotgun sequence.
ACCESSION   DATLKL010000215 DATLKL010000000
VERSION     DATLKL010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205258"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VM713_02790"
     CDS             complement(<1..356)
                     /locus_tag="VM713_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HVH83221.1"
                     /translation="MSEEKLSFQAEVSKLLDIVAHSLYSEREVFLRELISNASDACDR
                     LRYAALTKPELLEGDPGFRITLAFDKGAGTLTVADNGVGMSRQELIDNLGTIARSGTA
                     AFLAEAGTSSGADGLSL"
     gene            525..1223
                     /locus_tag="VM713_02795"
     CDS             525..1223
                     /locus_tag="VM713_02795"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HVH83222.1"
                     /translation="MIFNPLRRGGRPPRTIADLRDRLVAEVEAEVRETSRYLGKDALA
                     PAVVAALAKVPRQLFVPPEVEHLAYENAPLGIGYRQTISQPYIVAVMTDMLEPRPTGV
                     VLEIGTGSGYQAAVLAELVKQVYSIEIVPELATSAAERLDRLGYHNVQVREGDGALGW
                     PEHGPYDGILVTAAAPEIPEALLDQLRPGRRLVAPVGEAGEHQELMVVEKGPRGEMRE
                     RVTLPVAFVPLRAG"
     gene            1258..>1692
                     /locus_tag="VM713_02800"
     CDS             1258..>1692
                     /locus_tag="VM713_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83223.1"
                     /translation="MTHKDRPPIAVLGPTNTGKTHLALERMLGHRTGMIGFPLRLLAR
                     ENYDRMVRLKGAAAVALVTGEEKIVPAHPSHWVCTVEAMPLDRAVNFLAVDEVQVAAD
                     PERGYVFTDRLLRVRGLEETMFLGAETIRPLLKALVPKVEFVM"
BASE COUNT          279 a          554 c          583 g          276 t
ORIGIN      
        1 aggctgaggc catcggcgcc gctggacgtg cccgcctcgg ccaggaaagc ggcggtgccg
       61 gagcgcgcga tggtgccgag attgtcgatc agctcctgcc ggctcatgcc aaccccgttg
      121 tccgccaccg tgagggtccc agcacccttg tcgaaggcga gggtgatgcg aaaaccgggg
      181 tccccctcca gaagctcggg cttggtgagg gccgcatagc gcagccgatc gcaggcatcc
      241 gacgcgttcg aaatcagctc gcgcaggaac acctcccgct cgctgtagag ggagtgggcg
      301 acgatgtcga gcagcttgct aacctcggcc tggaagctca gcttttcctc ggacatgatc
      361 cccccgttat cgttccagca tgtctgggcg tgatatgagg aagggcagcg ccgtctacaa
      421 gcctctgggc aaagagctgc ccgtcgctgg gtggccagcg gcacggggcg ccggcgcaat
      481 tgaggcggct ggagccccgg ccccctgcct gctatgctga gaccgtgatc ttcaatcccc
      541 tgcgccgggg tggcaggccg ccccgcacga tcgcggacct tcgcgaccgt ctcgtcgcgg
      601 aagtggaggc cgaggtgcgg gagacgagcc gctacctcgg caaggacgcg ctggcacccg
      661 ccgtggtcgc ggcgcttgcc aaggtgccac gccagctctt tgtgccgccg gaggtcgagc
      721 acctggctta cgagaacgcg ccgctgggga tcggctaccg ccagaccatc tcgcagccct
      781 atatcgtcgc cgtgatgacg gacatgctag agccgcgtcc cacgggcgtt gtgctcgaga
      841 tcggcaccgg gagcggctac caggcggccg tgctggcgga actcgtcaag caggtctaca
      901 gcatcgagat cgtccccgag ctcgccacca gcgcggcgga gcggctcgac cggcttggtt
      961 accacaatgt ccaggtgcgc gagggcgatg gcgcgctcgg ctggccggag cacgggcctt
     1021 atgatggcat cttggtgacc gcggccgcgc cagagattcc cgaggcgctg ctggatcagc
     1081 tcaggcccgg ccgtcgtcta gtggctccgg tcggcgaggc cggcgagcat caggagctga
     1141 tggttgtcga gaaagggccc cggggcgaga tgcgcgagcg cgtaaccctg ccggtcgcct
     1201 ttgtgccgct ccgagccggc taagcggaaa ctagcggtaa aagcgattct cgaaaccatg
     1261 acgcacaaag accggccacc gatcgccgtc ctgggaccga ccaacacggg caagactcac
     1321 cttgcgctcg agcgcatgct tgggcatcgg accggcatga tcgggtttcc cctgcgactg
     1381 ctcgcacggg agaactatga ccgcatggtg cggctcaagg gcgctgcggc ggttgcgctc
     1441 gtaacgggcg aggagaagat cgttcccgcc cacccttcgc actgggtgtg cacggtcgag
     1501 gcgatgccac tcgaccgcgc ggtgaatttt ctcgcggtcg acgaggtcca agtcgcggcc
     1561 gaccccgagc gcggctacgt gtttaccgat cgcctgcttc gcgtgcgagg gctcgaggag
     1621 accatgttcc tcggcgccga gacgatccgg ccgcttctca aggccctcgt gcccaaggtc
     1681 gagttcgtga tg
//
LOCUS       DATLKL010000216         3410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_2454, whole genome shotgun sequence.
ACCESSION   DATLKL010000216 DATLKL010000000
VERSION     DATLKL010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2454"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VM713_02805"
     CDS             complement(<1..669)
                     /locus_tag="VM713_02805"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HVH83224.1"
                     /translation="MSGPTAVVFAYHDVGVRCLKALLSAGVQIPLVVTVADDPGENRW
                     YASVAETAADYGLACVAPAAAAAAGLETRLRALQPDFLFSFYYRSLLPAPLLTSARRG
                     ALNMHGSLLPKYRGRAPVNWAILKGESETGATLHYMVEQADAGDIVDQLAVPILIDDD
                     ARAVFAKVTVAAETILARSLPKLIAGSAARRPQTLVPGQYFGRRTPEDGRIDWSRPAR
                     EIHDL"
     gene            complement(666..1694)
                     /locus_tag="VM713_02810"
     CDS             complement(666..1694)
                     /locus_tag="VM713_02810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVH83225.1"
                     /translation="MNPAISVVIPVFNEQEGLPLLFERLYRALDALGRSYEVVFVDDG
                     SRDASVAELREQYQRRPDVTRVVVLARNAGQHRAILAAFSYTRGDYVITLDADLQNPP
                     EEIAKLVAAMDAGADYVGTIRVRRQDVMWRKAASRLMNRLREGTTSIRITDQGCMLRG
                     YHRSVIDAVNRCSEVSTYVPALAYTFARHPVEIEVAHAERTVGQSKYSLLRLIRLNFD
                     LMTGFSVAPLQFFSMSGIVIALLSLIFVAYLAIRRLIVGPEAEGVFTLFGIAFFLIGM
                     ALLGLGVVGEYVGRIYEQVRQRPRYTVAAVLGGEAEPAQLPAGARERGAEVTALPPPV
                     PRARGVPE"
     gene            complement(1691..2839)
                     /locus_tag="VM713_02815"
     CDS             complement(1691..2839)
                     /locus_tag="VM713_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HVH83226.1"
                     /translation="MNDSPKTPARFLPFTRPSIDEETIASVAEVLRSGWLASGPKVAA
                     LEAALSDYLGGRPVRTQTSATAGLEIALLACGIGPGAEVITPALSFVATANVIVRVGA
                     RPVFVDVGLDSRNLDLAQVEEAITPRTRAILPVHFAGMPVDMERLYAIAGRHGLRVIE
                     DAAHAIGSTWAGKRIGSFGDLVVFSFHPNKNITTIEGGAISGGSAAELRSIELHRWHG
                     QLKSGPDSFDTLLAGGKCNLSDVAAAVGLGQLRRLEEFNAKRRALVARYFELWKSAAP
                     LRLPELGDAGHSWHVFTPLLPLDTLSITRLAFIEAMRERGIGVGVHYPAIHTFSAYRA
                     LGYREGQFPNAERIGRETVTLPLFPAMELGDVERVVQTVNDIVRGALK"
     gene            complement(2836..3207)
                     /locus_tag="VM713_02820"
     CDS             complement(2836..3207)
                     /locus_tag="VM713_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVH83227.1"
                     /translation="MKLTDFSILFCGVLLNALAQLALKAATRVSGPLVATDSGVLRRG
                     LELLTVPSLWYALCAYGLSVIVWLVGLSRVPVSQAYPLLSLGYVINIGLAWWLLGEVP
                     NVQRVGGIAIIVVGVVLVSRS"
     gene            complement(3204..>3410)
                     /locus_tag="VM713_02825"
     CDS             complement(3204..>3410)
                     /locus_tag="VM713_02825"
                     /inference="COORDINATES: protein motif:HMM:NF037277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83228.1"
                     /translation="DYDGELAFGLRAEPGREDLSVAAFKARWDASRDAVAFFDPGIWD
                     SWRRAGFPGNVIAADNYTVAVSRL"
BASE COUNT          572 a         1187 c         1161 g          490 t
ORIGIN      
        1 caggtcgtga atctcgcgtg ctggccgtga ccagtcgatg cgaccgtcct ccggtgtgcg
       61 ccgcccgaag tattgtcccg ggacgagggt ctgtggccgt cgcgcggcgc tgccggcgat
      121 gagcttcggc agcgagcgcg cgaggatggt ctcggcggcg accgtgacct tggcgaacac
      181 cgcgcgcgca tcgtcgtcga tcaggatggg aaccgcgagc tgatcgacaa tgtcaccggc
      241 gtcggcctgc tccaccatgt agtggagcgt cgcaccggtc tcgctctcgc ccttcaggat
      301 cgcccagttg accggcgccc ggccccggta cttcggcagg agcgagccat gcatgttgag
      361 ggcaccacgg cgcgcactcg tgagcagcgg cgcaggcagc agcgagcggt aatagaagga
      421 gaagaggaaa tccggctgga gcgcgcgcag gcgggtctcg agcccggccg ctgcggccgc
      481 ggcgggcgcg acacacgcga gcccgtagtc ggcggcagtc tcggcgacgc tcgcgtacca
      541 gcggttctcg cccggatcgt cggcgacggt gaccaccagg ggaatctgca cgcctgcaga
      601 cagcagcgcc ttgaggcacc gcactccgac atcgtgatac gcgaagacga cggccgtcgg
      661 gccgctcact cgggcactcc ccgtgcgcgc ggtaccggag gcggcagcgc ggtaacctcc
      721 gcgccccgct cgcgcgcgcc ggccggcagc tgtgcgggct cggcctcccc gccgagcacc
      781 gccgcgaccg tgtagcgcgg ccgctgccgc acctgttcgt agatgcgtcc gacgtactcg
      841 ccgaccaccc cgagcccgag gagcgccatg ccgatcagga agaaggcgat cccgaacagc
      901 gtaaacacgc cctccgcttc cggacccacg atcaggcgac ggatcgcgag gtaagcgacg
      961 aagatgagcg acagcagcgc gatgacgatg ccgctcatcg agaagaactg cagcggcgcg
     1021 accgagaagc cggtcatcag gtcgaagttg aggcgaatga gccgcagcag cgagtacttg
     1081 gactggccga ccgtgcgctc ggcatgcgcc acttcgatct cgaccgggtg gcgcgcgaag
     1141 gtataggcga gcgccggcac ataagtgctc acctcgctac agcggttgac cgcgtcgatg
     1201 acgctgcggt gatagccccg cagcatgcaa ccctggtcgg tgatgcggat cgaggtggtg
     1261 ccctcgcgca gccggttcat gaggcgcgac gccgccttgc gccacatgac gtcctggcgc
     1321 cgcacgcgga tcgtgccgac gtagtccgcg cccgcgtcca tcgccgctac cagcttagct
     1381 atctcctcgg gcggattctg caggtcggcg tcgagcgtga tgacgtagtc gccgcgcgtg
     1441 tagctgaagg cggcgagtat cgcgcgatgc tggccggcgt tgcgcgccag taccacgacc
     1501 cgcgtgacgt cagggcgccg ctggtactgc tcgcgcagct ccgccaccga agcgtcgcgg
     1561 ctgccgtcgt cgacgaatac cacctcgtag ctgcggccga gcgcatcgag cgcccggtac
     1621 agccgctcga acagcagcgg cagcccctcc tgctcgttga agaccggaat caccaccgag
     1681 atcgccgggt tcacttcagt gctccgcgta cgatgtcgtt caccgtctgc acgacccgct
     1741 cgacgtcgcc gagctccatc gccgggaaca gcggcagcgt gacggtctcc cggccgatgc
     1801 gctcagcgtt gggaaactga ccctcgcgat agccgagcgc gcgataggcg ctgaaggtgt
     1861 gaatcgccgg gtagtgcacg ccgacgccga tgccgcgctc gcgcatcgcc tcgatgaagg
     1921 cgagtcgcgt gatcgacagc gtgtcgagcg gcagcagcgg cgtgaatacg tgccagctgt
     1981 gtcccgcatc gccgagctcc ggcagccgca gcggcgcggc gctcttccac agctcgaagt
     2041 agcgcgcaac cagcgcgcgc cgtttggcgt tgaattcctc gagccgccgc agctgtccga
     2101 ggccgactgc ggcggcgaca tccgacaggt tgcacttgcc gccggcgagc aatgtatcga
     2161 aggagtcggg gccggacttc agctgcccgt gccagcggtg cagctcgatg ctgcgcagct
     2221 ccgcggccga gccccccgag atcgcgccgc cttcgatggt ggtgatgttc ttgttcgggt
     2281 ggaagctgaa gaccaccagg tcgccgaagc tgccgatacg cttacccgcc cacgtcgagc
     2341 cgatggcgtg cgccgcgtcc tcgatcaccc gcagcccatg gcggccggca atcgcataga
     2401 gccgctccat gtcgaccggc atcccggcga agtgcaccgg caggatggcg cgggtgcgcg
     2461 gggtaatcgc ctcttcgacc tgcgcgaggt cgagattgcg cgagtcgagg ccgacgtcga
     2521 cgaacaccgg gcgcgcgccc acccgtacga tcacgttggc ggtcgcgacg aagctcagcg
     2581 cgggcgtgat gacctcggcc cccgggccga tcccgcaggc gaggagcgcg atttccaggc
     2641 cggcggtcgc cgaggtctgc gtgcgcaccg gccgcccgcc gagataatcc gatagcgcgg
     2701 cctcgagtgc cgccaccttc ggtccgctcg ccagccaccc cgagcgcagc acctcggcca
     2761 ccgacgcgat ggtctcctcg tcgatgctgg ggcgggtgaa gggcaggaac cttgccgggg
     2821 tcttgggcga atcgttcatg agcgcgacac cagtacgact cccaccacga tgatcgcgat
     2881 gcccccgacg cgctggacgt tgggcacctc gccgagcagc caccacgcga gtccgatgtt
     2941 gatcacgtag ccgagtgaca gcagcgggta cgcctggctc actggcacgc gcgacaggcc
     3001 gaccagccac acgatgacac tgagtccgta ggcgcacaat gcataccaca gcgacggcac
     3061 tgtcagcagc tcgagtccgc gccgcagcac tccgctgtcg gtggccacga gcggtcccga
     3121 cacgcgggtg gcggccttga gagccagctg cgccagagcg ttcagcagga cgccgcagaa
     3181 caggatcgag aaatcggtca gtttcacaga cgactcactg caacggtgta gttatcggcg
     3241 gcgatcacgt tgccgggaaa ccccgcgcgc cgccaggaat cccagatccc ggggtcgaag
     3301 aatgccacgg cgtcgcggct cgcgtcccag cgggccttga aggccgcgac gctcaggtcc
     3361 tcccgcccgg gctccgcccg cagcccgaag gcgagctcgc catcgtaatc
//
LOCUS       DATLKL010000217         3390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_194103, whole genome shotgun sequence.
ACCESSION   DATLKL010000217 DATLKL010000000
VERSION     DATLKL010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194103"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..833)
                     /locus_tag="VM713_02830"
     CDS             complement(48..833)
                     /locus_tag="VM713_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH83229.1"
                     /translation="MMDVIIVDDEPAARRTVRECCEREGDFNVIGEFGDGRAALEAIR
                     ARPPHVLFLDIQMDSLTGMALARALDPVTLPLIVFVTAYDHYALEAFEVSAVDYLLKP
                     FDDARFKATAARVRRRREGTSSTDRQRALESLLEQLERGARPHDIQPRVLAEAGSRMH
                     MLDVAQVEVVEADRNYVRLTIGREVFHARSTLQQAERSFQTQPMLRISRSCLVNMSHV
                     KEVSRTPRGDFILVVVGGTTVTSSEGFRDPVRQYLERLKLAPA"
     gene            complement(830..2077)
                     /locus_tag="VM713_02835"
     CDS             complement(830..2077)
                     /locus_tag="VM713_02835"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVH83230.1"
                     /translation="MSASPLERISPAFLGVARDGDAADERRAARARRNFWPQLAVLTG
                     FWIYVALSNLMYANTMGESLKSLNVADFFAPWDARLIQHLILYPLFILCAWTSLRIGW
                     RPLWRTLPIQLSCGLGFAVLAAPALVAGEVIMGEWHGTGGITHPLQKSYENWADFFAG
                     PAVPTWIASATSFVVTYGFGLALITSFGFYQRLRDSQLRSAALERALAAAHLAALRMQ
                     LSPHTLFNLLHTIRGQITWDPPAAQAMVVQLGDLLRRLLTVGEQEFSRLGDELQFVQL
                     YLQLQQRRFADRLSITVPPRDAAPLAWVPSLILQPLVENAVVHGLGGHDGAVSVRVEA
                     SVVGETLVLRVVNNLAPSWHGLHGPGIGLANVRERLSVQFGARASFTAGPGDDHQWVA
                     EIRIPLLRDGPEAAPRAPPDADQ"
     gene            2346..2705
                     /locus_tag="VM713_02840"
     CDS             2346..2705
                     /locus_tag="VM713_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HVH83231.1"
                     /translation="MTSATLNFGARLRSAVAFTVLAAGTAIGAVGNAHAATNADAPTL
                     KVRYADLNLSTDQGSRVLYGRIVAAARQVCAVEDIRDLRAFRAAKACREQAIAQAVRD
                     VNSPMLAAVHAEQLRHG"
     gene            complement(2908..3282)
                     /locus_tag="VM713_02845"
     CDS             complement(2908..3282)
                     /locus_tag="VM713_02845"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HVH83232.1"
                     /translation="MTDRAFDLNTWLETYKETVASFSKAQQEGFRALERFARFNYAVA
                     GDCLEAGLAQAKAALSARAAVGTQAVADLLAKQAELSNELSEKLRARAQEFSGLAAEV
                     QESVSTFAAEAATRAAGTKKAA"
BASE COUNT          560 a         1151 c         1154 g          525 t
ORIGIN      
        1 atgcttactc ccctcgcggt gagtcagctt gggactttag cgcagtttta cgccggcgcc
       61 agtttcagcc gctcgaggta ctggcggacc ggatcgcgaa agccttcgct gctcgtgacg
      121 gtcgtgccgc cgacgaccac caggatgaag tcgccgcgcg gcgtgcggct gacctccttc
      181 acgtgactca tgttcaccag acaggagcgg ctgatgcgca gcatcggctg cgtctggaag
      241 gagcgctcgg cctgctgcag cgtactgcgg gcgtgaaaca cctcccggcc gatggtgaga
      301 cgcacgtagt tgcggtcggc ctcgaccacc tcgacctgcg ccacgtccag catgtgcatg
      361 cggctgccgg cctcggccag cacgcgcggc tggatatcgt gggggcgcgc gccgcgttcg
      421 agctgctcca gcagcgactc gagcgcgcgc tgccggtcgg tgctgcttgt tccctcgcgc
      481 cggcggcgca cgcgcgccgc ggtcgccttg aagcgcgcgt catcgaaggg cttgagcaga
      541 tagtcgaccg cgctcacctc gaaggcctcg agcgcatagt ggtcgtaggc cgtcacgaac
      601 acgatcagcg gcagcgtcac cgggtcgagc gcgcgcgcca gcgccatgcc ggtcagcgag
      661 tccatctgga tgtcgaggaa cagcacgtgc ggcggccgcg cccggatcgc ctcgagcgcg
      721 gcgcggccgt cgccgaactc cccgatcaca ttgaagtccc cttcgcgctc gcaacactcg
      781 cgcaccgtgc ggcgcgccgc cggctcgtcg tcgacgatga tgacgtccat cattgatcgg
      841 catccggcgg cgcacgcggc gccgcctccg gcccgtcgcg cagcagcggt atgcggatct
      901 ccgccaccca ctggtgatca tcgccggggc cggccgtgaa gctggcgcgg gcgccgaact
      961 gcaccgacag ccgctcgcgc acgttggcga gtccgatccc tggcccgtgc agtccgtgcc
     1021 aggagggtgc gaggttgttg acgacccgca gcaccagagt ctcgccgacg acgctcgcct
     1081 cgacgcgcac gctcaccgca ccgtcgtggc ccccgagccc gtgcaccacc gcgttttcca
     1141 ccagtggctg caggatgagg ctcggcaccc acgcgagcgg tgcggcgtcg cggggcggca
     1201 ccgtgatgct cagccgatcg gcgaagcgcc gctgctgcag ttgcagatag agctgcacga
     1261 actgcagctc gtcgccgagg cgcgagaact cctgctcgcc caccgtcagc agacggcgca
     1321 gcaggtcgcc aagctgcacc accatcgcct gcgcggcggg cggatcccag gtgatctgcc
     1381 cgcggatggt atgcagcagg ttgaagagag tgtgcggcga cagctgcatg cgcagcgccg
     1441 cgaggtgcgc cgcggcgagt gcgcgctcga gcgccgccga gcgcagctgc gagtcgcgca
     1501 gccgctgata gaagccgaag ctggtgatga gcgccagtcc gaagccgtag gtgaccacga
     1561 agctcgtggc gctcgcgatc caggtcggta ctgcgggacc ggcaaagaaa tcggcccagt
     1621 tctcgtagct cttctgcagg gggtgcgtga tgccgcccgt gccgtgccac tcacccatga
     1681 tcacctcgcc ggccaccagc gccggcgccg cgagcaccgc gaagccgagc ccgcaggaga
     1741 gctggatggg gagcgtgcgc cacagcggcc gccagccgat gcgcagcgac gtccaggcgc
     1801 acaggatgaa gagcggatag aggatcagat gttgaatgag ccgcgcgtcc cagggagcga
     1861 agaagtcggc gacgttgagc gacttcaggc tctcgcccat ggtgttggcg tacatgaggt
     1921 tcgagagcgc gacgtagatc cagaaaccgg tgagcaccgc cagctgcggc cagaagttgc
     1981 ggcgcgcgcg cgcggcgcgg cgctcgtcgg cggcgtcacc gtcgcgcgca acgccaagga
     2041 atgcgggact gattcgctcg agtggagagg cgctcattcc tgagagcata gcctacgccc
     2101 aacccttccc ccgtgccgca gttgcacgcg ttgggacgat ttcgtcgatc cgcgggacga
     2161 atccgcgccg cgcctgtcgc acgccgcgcg gcccacggcg ccgcggaaag ccacgcccgc
     2221 cgtgcggtac gaagtcgtcg ctctccggga tcaatccgtc gcgcgccgtc tttaaacgga
     2281 acgcgcacct tgcgaatctg atcatcagcg tcgcgatggt cgtgacgcac aggaaggaga
     2341 agcccatgac ctctgcaacg ctcaacttcg gcgctcgcct gcgcagtgcc gtggccttta
     2401 cagttctcgc cgccggcacc gccatcggcg ccgtcggcaa cgctcatgcc gccactaacg
     2461 ccgacgctcc gacgctcaag gtccgctatg cggatctgaa tctctcgacc gaccagggat
     2521 cgcgcgtgct ctacggacgc atcgtcgcgg cagcgcgcca ggtgtgtgcg gtcgaggaca
     2581 tccgcgatct gcgggccttt agagcagcga aggcctgccg cgagcaggca atcgcgcagg
     2641 ccgtgcgcga cgtcaacagc ccgatgctcg ccgcggtgca cgccgagcag ctgcggcacg
     2701 gctgaggaaa cgcgccctgg aggatcgtgg cgctaacacg gatcgtgcac tcgcacgggt
     2761 gacgtggcag ggaccagtca cccgtgcgag aggactcagc cgccgcacct cgcgcgagcc
     2821 gcgcgcaagc gcagacgaac aaaaaaaggg cgaccccgac ggggatcgcc ctttgattta
     2881 tgagtggtgt ccgactgagc gggctgctta cgcggccttc ttcgtgccag cggcgcgcgt
     2941 ggcggcctcc gcggcgaagg tgctgaccga ctcctgcact tcggcggcca gacccgagaa
     3001 ctcctgggcg cgcgcgcgca gcttctcgct cagctcgttg ctgagctcgg cctgtttggc
     3061 gagcaggtcg gccacggcct gagtgccgac tgcagcgcgg gcgctgagag ccgccttggc
     3121 ctgggcgaga cccgcctcga ggcagtcacc tgcgacggca tagttgaagc gggcaaatcg
     3181 ctcgagcgct ctgaagccct cctgctgggc cttgctgaag gaggcgacgg tctccttgta
     3241 ggtctcgagc caggtgttga ggtcaaacgc gcggtcggtc atggcagctg ctccgaagac
     3301 agttggggag tttatgccgg ccattctaca cccgtttgta tgcattgcaa tatgaaaccg
     3361 aacgccgcgt catgtcagaa tatattgttt
//
LOCUS       DATLKL010000218         4734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_194146, whole genome shotgun sequence.
ACCESSION   DATLKL010000218 DATLKL010000000
VERSION     DATLKL010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194146"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1851)
                     /gene="nuoG"
                     /locus_tag="VM713_02850"
     CDS             complement(<1..1851)
                     /gene="nuoG"
                     /locus_tag="VM713_02850"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HVH83233.1"
                     /translation="MADDLVNIEIDGVPVQARKGEMIIRAADRHGIYVPRFCYHEKLS
                     VAANCRMCLVEVEKAPKPQPACATPVGPGMKVFTKSPRAIGAQRAVMEFLLINHPLDC
                     PICDQGGECELQDLAVGFGRDASRYNERKRVVADENLGPLIATDMTRCIHCTRCIRFT
                     EEVAGIQELGMIGRGEHMKVRTYIESAVNHELVGNVIDLCPVGALVSKPYRFSARAWE
                     MTAAPLVSPHDAVGSNLYGHVLRGRLMRVVPRENEAINETWIADRDRFSYEGVYSPDR
                     LERPLMRRGPDWVETDWESVLEQVAQGLKARAPACGFLVSPSATLEELYLAGRIARGL
                     GTHNIDHRLRQRDFRDAAADPATPGLGMRIADVDALNALLIIGSNLRREVPLLAHRVR
                     KAAGRGAQIAFVNPVRFAYQFPVTAYLSSAPRTLVGDLAAILAAVAAAQGKPVPAHLS
                     AATAAAQVNEEHRAVARALLSGEKRAVWIGALALRHPQLADLRSLAAALAQLAGAAFG
                     VLAEGANGAGAYLAGAVPHREAGAKAVAQPGLTAREMLQKPLKACVLFGGVEPSIDAL
                     APDSASVFARSELVVALTPFVSEELKRIAHVLLPIGTFAETLGTYVNCEGL"
     gene            complement(1853..3181)
                     /gene="nuoF"
                     /locus_tag="VM713_02855"
     CDS             complement(1853..3181)
                     /gene="nuoF"
                     /locus_tag="VM713_02855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HVH83234.1"
                     /translation="MNDLERKYADKMNLVAFEPLKLERSWTLPVYRSIGGYEVWERIL
                     REKPDRGSIIEQVKASGLRGRGGAGFPTGTKWSFMPRNSPVQKYLVCNSDESEPGTCH
                     DRDILRYNPHALIEGLAIAGYATNSTVAYNYIRGEFLGEPAPRFEAALAESYEAGLLG
                     SNIRGSGVDFDLHAFIGAGAYICGEETGLLESLEGKPGKPRFKPPFPANYGLYGAPTT
                     INNTLSFASVPTILRKGPAWFAALGPANSGGTLIFSFSGHVAKPRNFELPLGTPFADL
                     LALNGGMRGGRKLKAVIPGGVSVPVVPGEIMMKTNMDYDAVKAAGSALGTGAAIIMDE
                     TTCMVRVLERVSRFYMSESCGQCSPCREGTGWMNRLLKRMLAGEARREELGLLLDVAN
                     RIEGHTICAFGDASAWPVQSFLKHYRHEFEYVIDHGGRSLVTDTLGAQAA"
     gene            complement(3178..3714)
                     /locus_tag="VM713_02860"
     CDS             complement(3178..3714)
                     /locus_tag="VM713_02860"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HVH83235.1"
                     /translation="MSNGRSSVGVAGTALMSKLSLLSEATRREIDHWVAKFPPGRQRS
                     ASIAALRAAQEQNDGHLTPDLMDAVAEYLQLPPIQVYEVASFYSMFETQPCGRHHVSI
                     CTNISCMLNGAEALVAHAEKKLGIRLNESTSDGRIFLKREEECLAACNGAPMMMVDHV
                     FHENLTPERLDEVLDELK"
     gene            complement(3711..>4734)
                     /locus_tag="VM713_02865"
     CDS             complement(3711..>4734)
                     /locus_tag="VM713_02865"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HVH83236.1"
                     /translation="YVLAIEQLLGVSPPERAQYIRVMFDEITRILNHLMFLGSHALDI
                     GAMTVFLLCFKEREELLDVYEAVSGTRMHATYYRPGGVYRDLPDSMPKYQVTRWHSAK
                     DVARMNAARSGTMLDFIQAFTEQFPAAIDDYEALLTDNRIWKQRTVNIGVVTAERAMQ
                     LGFSGPMLRGSGVAWDLRKKQPYEVYDRMDFDVPVGVNGDCYDRYLVRVEEMRQSNRI
                     IRQCLEWLRAHPGPVMVDDTKVRPPSRERMKEDMESLIHHFKFFTEGYEVPVGATYAA
                     VEAPKGEFGVFLISDGANKPYRLKCRAPGFPHLAALEEMCTGHMLADVVAVIGTQDIV
                     FGEIDR"
BASE COUNT          731 a         1608 c         1630 g          765 t
ORIGIN      
        1 cagcccctcg cagttcacgt aggtccccaa ggtctcggca aaggtgccga tcggcagcag
       61 gacgtgcgcg atgcgcttga gctcctcgct cacgaacggc gtcagcgcca cgacgagctc
      121 gctgcgggca aacacgctcg cgctgtccgg ggcgagcgcg tcgatcgagg gttcgacgcc
      181 gccgaagagc acacaggcct tgagcggctt ctgcagcatc tcccgcgccg tgagccccgg
      241 ctgcgccacc gccttggctc ccgcctcgcg gtgcggcacc gccccggcga ggtaggcgcc
      301 cgcgccgttc gcgccctcgg cgagcacgcc gaaggcggcg ccggcgagct gcgcgagcgc
      361 cgcggcgagg gagcgcaggt cggcgagctg cggatggcgc agggcgagcg ccccgatcca
      421 taccgcgcgc ttctcgccgc tgagcagcgc acgggccacg gcgcgatgct cctcgttgac
      481 ctgggcggcc gcggtagcgg cgctcagatg ggcaggtacc ggcttgccct gggccgcggc
      541 gaccgcggcg aggatcgccg ccaggtcgcc caccagggtg cgtggcgccg aggacagata
      601 ggccgtcacc gggaactggt aggcgaagcg caccgggttc acgaacgcga tctgtgcgcc
      661 gcggcccgcc gccttgcgca cccggtgcgc cagcagcggc acctcgcgcc gcaggttcga
      721 gcctatgatg agcagcgcat tcagcgcatc gacgtcggcg atgcgcatcc cgagccccgg
      781 ggtcgccggg tcggcggcgg catcccgaaa gtcgcgctgc cgcagccggt gatcgatgtt
      841 gtgagtgccg agcccgcggg cgatgcgccc ggcgaggtac agctcttcca gcgtcgccga
      901 ggggctcacg aggaagccgc acgcgggggc gcgggctttg agaccctgcg ccacctgctc
      961 gaggacactc tcccagtccg tctcgaccca gtcgggcccg cgccgcatca gcgggcgctc
     1021 gagccggtcg gggctgtaga cgccttcgta gctgaagcga tcgcgatcgg cgatccaggt
     1081 ctcgttgatg gcctcgttct cgcgcggcac gaccctcatg agccgcccgc gcagcacgtg
     1141 accgtacagg ttgctgccga ccgcatcgtg cggcgagacc agcggcgccg cggtcatctc
     1201 ccaggcgcgc gcgctgaagc ggtagggctt ggagaccagc gccccgaccg gacagaggtc
     1261 gatgacgttg ccgaccagct cgtgattgac cgcgctctct atataggtac gcaccttcat
     1321 gtgctcgccg cggccgatca tgccgagctc ctggatgccg gcgacctcct cggtgaagcg
     1381 gatgcagcgc gtgcagtgaa tgcagcgcgt catgtcggtg gcgatcagcg gaccgaggtt
     1441 ctcgtccgcc accacgcgct tgcgctcgtt gtagcgcgag gcgtcgcggc cgaagccgac
     1501 cgccagatcc tgcagctcgc actccccgcc ctgatcgcag atcgggcagt cgagcgggtg
     1561 gttgatcagc agaaactcca tgaccgcacg ctgggcgccg atggcgcgcg gcgacttggt
     1621 gaacactttc atgcccggac cgaccggcgt agcgcaggcc ggctgcggct tgggcgcctt
     1681 ctcgacctcg accaggcaca tgcgacagtt ggccgctacc gagagcttct cgtggtagca
     1741 gaaacgcggc acatagatgc cgtggcgatc ggcggcgcgg atgatcatct cgcccttcct
     1801 ggcctgcacc ggtacgccgt cgatctcgat attgaccagg tcgtctgcca tgtcaggccg
     1861 cctgcgcgcc gagcgtgtcg gtcacgagac tgcgcccgcc gtgatcgatg acgtactcga
     1921 actcgtggcg gtagtgcttg aggaagctct gcaccggcca ggcagaggcg tcaccgaagg
     1981 cgcagatggt gtgtccctcg atgcggttgg cgacatctag gaggagtccg agctcctcgc
     2041 ggcgcgcctc acccgccagc atccgcttca gcagccggtt catccagccg gtgccctcgc
     2101 ggcacggcga gcactgcccg caggactccg acatgtagaa gcgcgagacg cgctccagca
     2161 cccgcaccat gcaggtggtc tcgtccatga tgatggcggc gccggtgccg agcgccgagc
     2221 ccgccgcctt caccgcgtcg tagtccatgt tggtcttcat catgatctcg cccggcacca
     2281 ccggcacaga caccccgccc gggatcacgg ccttgagctt cctgcccccg cgcatgccgc
     2341 cgttgagggc cagcaggtcc gcgaacggcg tgccgagcgg cagctcgaag ttgcgcggct
     2401 tggccacgtg cccggagaag gagaagatca gcgttccgcc cgagttggca ggcccgagag
     2461 ctgcgaacca cgccggaccc ttgcgcagga tggtcggcac cgaggcgaag ctcagcgtgt
     2521 tgttgatggt ggtgggcgca ccgtacagcc cgtagttcgc cggaaacggc ggcttgaagc
     2581 gcggcttgcc gggcttgccc tcgagcgact cgaggagccc ggtctcctcg ccgcagatgt
     2641 aggcgccggc gccgatgaaa gcatggaggt cgaagtcgac acccgagccg cggatgttgc
     2701 tgccgaggag tccggcctcg taggactcgg cgagcgctgc ctcgaagcgc ggggccggct
     2761 cgccgaggaa ctccccgcgg atgtagttgt aggcgaccgt tgagttggtg gcgtagccgg
     2821 cgatcgccag cccctcgatg agggcatgcg ggttgtagcg caggatgtcg cggtcgtggc
     2881 aggtgccggg ctcgctctca tcggagttgc acaccaggta cttctgcacc ggcgagttgc
     2941 gcggcatgaa gctccacttg gtgccggtgg gaaagccggc gccgccgcgc ccgcgcagtc
     3001 ccgaggcctt gacctgctcg atgatggagc cgcggtcggg cttctccctg aggatgcgct
     3061 cccacacctc gtagccgccg atcgaacggt agaccggcag cgtccaggag cgctcgagct
     3121 tcaggggctc gaaagccacg aggttcatct tgtcggcgta cttgcgctcg aggtcgttca
     3181 cttcagctcg tccaggactt catcgagccg ctcgggcgtc aggttctcgt ggaatacgtg
     3241 atccaccatc atcatcggcg cgccgttgca ggcggcgagg cactcctcct cgcgcttgag
     3301 gaagatgcgc ccgtcggacg tgctctcgtt gagccggatg ccgagcttct tctccgcgtg
     3361 ggccacgagc gcctcggcgc cgttcagcat gcaggagatg ttggtgcaga tgctgacgtg
     3421 atggcgacca cagggttgcg tctcgaacat cgagtagaag ctcgctacct cgtagacctg
     3481 gataggcgga agctgcaggt actcggcgac cgcatccatc aggtccggcg tcaggtggcc
     3541 gtcgttctgc tcctgcgccg cgcgcagtgc cgcgatcgac gccgagcgct gccgcccggg
     3601 cgggaacttc gccacccagt gatcgatctc ccgccgcgtc gcctccgaca gcagcgagag
     3661 cttgctcatg agcgcggtgc cggcgacgcc gacgctgctg cgtccgttgc tcatcggtcg
     3721 atctctccga agacgatgtc ctgcgtgccg atcaccgcca ccacgtccgc cagcatgtgc
     3781 cccgtgcaca tttcctcgag cgccgccagg tgcgggaagc caggcgcgcg gcacttgagc
     3841 cgatagggct tgttggcgcc gtcggagatc aggaagacgc cgaactcgcc cttcggcgcc
     3901 tcgaccgccg cgtaggtcgc gcccacgggt acctcgtagc cctcggtgaa gaatttgaag
     3961 tggtggatga gggactccat gtcctccttc atgcgctcgc gcgatggcgg ccgcaccttg
     4021 gtgtcatcga ccatcaccgg acccgggtgg gcgcgcagcc actcgaggca ctgccggatg
     4081 atgcggttcg actggcgcat ctcctcgacc cgcaccaggt agcggtcgta gcagtcgccg
     4141 ttgacgccca ccggcacgtc gaagtccatg cggtcataga cctcgtaggg ctgcttcttg
     4201 cgcaaatccc aggcgacgcc ggagccgcgc agcatgggac cggagaagcc gagctgcatc
     4261 gcgcgctcgg cggtcacgac gccgatgttg accgtgcgct gcttccagat gcggttgtcg
     4321 gtgagcagcg cctcgtagtc gtcgatggcg gccggaaact gctcggtgaa cgcctggatg
     4381 aaatcgagca tcgtgccgct gcgcgcggcg ttcatgcgcg cgacgtcctt ggccgaatgc
     4441 cagcgcgtga cctggtactt cggcatcgag tccggcaggt cgcggtagac cccgccggga
     4501 cggtagtagg tggcgtgcat gcgcgtgccc gagaccgcct cgtacacgtc cagcagctcc
     4561 tcgcgctcct tgaagcacag caggaacacg gtcatcgcgc cgatgtcgag ggcgtgcgag
     4621 ccgaggaaca tgaggtgatt caggatgcgc gtgatctcgt cgaacatcac gcggatgtac
     4681 tgcgcgcgct ccggcggact caccccgagc agctgctcga tggcgagcac gtag
//
LOCUS       DATLKL010000219         2527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_160409, whole genome shotgun sequence.
ACCESSION   DATLKL010000219 DATLKL010000000
VERSION     DATLKL010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160409"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..461
                     /locus_tag="VM713_02870"
     CDS             141..461
                     /locus_tag="VM713_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HVH83237.1"
                     /translation="MKTHEHLTPRQEALTERNLCVALAALKSPEEVRAFLRDLCTPTE
                     IQAMADRWAVVDHLEHGLPYREIRRRTSVSLMTIARVARFLRSGNGGYLTAARRLGRG
                     EAAA"
     gene            472..1368
                     /gene="hisG"
                     /locus_tag="VM713_02875"
     CDS             472..1368
                     /gene="hisG"
                     /locus_tag="VM713_02875"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HVH83238.1"
                     /translation="MDEPRLKLAVQKSGRLTDPSLDLLARCGLKLSRGKDQLFGHGEN
                     MPLDVLFVRDDDIPDLVREDVCDLGLVGLNVLEEKRLALRHRTSDETLRPLRSLEFGR
                     CRLSLAIPEGCSYVGLASLTGRRIATTYPYVLEQYLHDRKVNAEIVTLSGSVEIAPRL
                     GRADLICDLVSTGSTLQANHLTEVETVLESHAVLIRTPVELPRAKAEWVERLLMRIDG
                     VQQVHESKYIMLHAPRAALAEIRRLLPGSESPTIIPLEGCADRVAVHAVCRENVFWET
                     LESLKKVGASALLVLPVEKMLA"
     gene            1383..>2527
                     /gene="hisD"
                     /locus_tag="VM713_02880"
     CDS             1383..>2527
                     /gene="hisD"
                     /locus_tag="VM713_02880"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HVH83239.1"
                     /translation="MRILDWTSLDEAGRRAALARPSREARAGVERLARDVIARVRRDG
                     DAALRTLTKRFDGVELDRFAVGPGEFSVARNSLSRAQLAALERAIANIQRFHQAQAPK
                     PLLVETEAGVRCEQIIRPIPSVGLYVPAGTAPLPSAVVMLGVPSRIAGVPRRILCTPP
                     QRDGLVHPAVLMAAELCGINEVFKVGGAQAIAALAFGTESIPRVDKIFGPGNAWVTAA
                     KTLVASDPEGAACDLPAGPSEVLIVADETAECELVAADLLAQAEHDSFAQTILVTHNR
                     ALIERLIGEIARQRAALSRGAILDESLSSCRLILVPDLETAICVVNLYAPEHLMLEVR
                     DSRRWLHEVQNAGSIFLGPWSAEPFGDYCAGPNHVLPTDGHARTVSGL"
BASE COUNT          414 a          845 c          817 g          451 t
ORIGIN      
        1 acaagtaggg ctcgtcgtct tattagtagt gctgagcacc taagcccttc gccggcccta
       61 accagctctt ctttgagaat agccggccgc tatgtgcgaa ttgggttgtg tttgttctaa
      121 tgtgctagta cattagaaca atgaagactc atgagcatct caccccgcgc caggaggcgc
      181 tcaccgagcg caacctgtgc gtggcgctcg cggctctcaa gagtcccgag gaagtgcgtg
      241 cgttcctgcg cgacctctgc accccgaccg agatccaggc catggcggac cgctgggctg
      301 tggtggatca cctggagcac ggcctgccct atcgcgagat ccgccggcgc accagcgtga
      361 gcctcatgac aatcgcccgc gtggcgcggt tcctgcgctc cgggaacggc ggctacctga
      421 ccgccgcacg gcgcctgggc cgaggggaag cggccgcctg aaggtcccga catggacgaa
      481 ccccgactca agcttgccgt ccagaagtca ggacggctca ccgatccctc gctcgacctc
      541 ctcgcgcgtt gcggactcaa gctctcacgc ggcaaggatc agctcttcgg tcacggcgag
      601 aatatgccgc tcgacgtcct cttcgtgcgc gacgatgaca ttccggatct ggtgcgggag
      661 gacgtctgtg acctgggtct cgtcgggctc aacgtgctgg aagagaagcg cctggcgctc
      721 aggcaccgaa catctgatga gactctgagg ccgctgcgca gcctcgaatt cgggcgctgc
      781 cggctctcgc tcgcgattcc cgaaggctgc tcctatgtcg gcctggcctc gctcaccggc
      841 cgacgcattg ccactaccta cccttacgtg ctcgagcaat accttcacga ccggaaagtg
      901 aacgccgaga tcgtgacgct ctcgggctcg gtcgagattg cgccgagact cggacgcgcg
      961 gacctcatct gcgatctcgt ttcgaccggc tcgacgcttc aggccaatca cttgaccgag
     1021 gtcgagaccg tgctcgagag ccacgcggtg cttatccgca ctccggtcga gctgccacgt
     1081 gccaaggccg agtgggtcga gcgtctcctg atgcgcatcg acggcgtgca gcaggtgcac
     1141 gagagcaagt acatcatgct gcatgcgccc cgtgccgcgc tagcggagat ccgccggcta
     1201 ttgccgggct cggagtcgcc cacgatcatt ccgctggaag gctgcgccga ccgggtggcc
     1261 gtgcacgccg tctgccgcga gaacgtcttc tgggagacac tcgagtctct gaagaaagtc
     1321 ggcgcgagcg cgctcttggt cctgccggtc gagaagatgc tggcctagag aataccgccc
     1381 ccatgcgcat cctcgactgg accagcctcg atgaggccgg gcggcgcgcc gccctcgccc
     1441 ggccctcgcg tgaagcacgc gcaggagtcg agcgcctcgc gcgcgatgtc atcgccaggg
     1501 tacgtcgcga cggcgacgcg gctcttagaa cactgacgaa gcgcttcgat ggagtcgagc
     1561 tggaccgctt cgccgtaggc cccggagagt tttccgtggc tcgcaacagc ctgagccgcg
     1621 cccagctcgc agcgctcgag cgggcgatcg cgaatattca gcgcttccat caggcacagg
     1681 cgccgaagcc cttgctggtc gagaccgagg cgggtgtgcg ctgcgagcag atcatccggc
     1741 cgattccctc ggtggggctc tacgtgccgg cgggtacggc tccgctcccc tccgcggtcg
     1801 tgatgttggg ggtaccttcg cgcattgcgg gagtgcctag gcggatcctc tgcacgccgc
     1861 cacagcgcga tgggctggtg catcccgccg ttctgatggc ggccgagctg tgtggcatca
     1921 atgaagtctt caaggtgggc ggtgcccagg cgatcgcagc gctcgcgttc ggcaccgagt
     1981 cgatccccag ggtcgacaag atcttcgggc ccggcaacgc ctgggtcacc gcggcgaaga
     2041 ccctggtcgc cagcgatccg gagggcgcgg cgtgcgatct gcccgcggga ccctccgagg
     2101 tgctgatcgt cgcagacgag acggccgagt gcgagctcgt ggccgccgat ctcctggcgc
     2161 aagcggagca cgattcgttc gcccagacga tcctcgtcac ccacaaccgc gcgctgattg
     2221 agcggctcat cggggagatc gccaggcagc gtgccgcgct ctcgcggggc gcgatcctgg
     2281 atgagtccct gagctcttgt cggttgatcc tggtgccaga tctcgagacc gccatttgcg
     2341 tggtcaatct ctacgcgccc gagcatctca tgctcgaggt gcgcgactcc cgccgctggc
     2401 tgcatgaagt acagaacgcg ggatcgatct tcctggggcc ctggtccgca gaacccttcg
     2461 gggactactg cgcaggtccc aatcacgtgc tccccaccga cgggcatgcg cgcactgtaa
     2521 gcggtct
//
LOCUS       DATLKL010000220         2758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_216667, whole genome shotgun sequence.
ACCESSION   DATLKL010000220 DATLKL010000000
VERSION     DATLKL010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216667"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            575..1249
                     /locus_tag="VM713_02885"
     CDS             575..1249
                     /locus_tag="VM713_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83240.1"
                     /translation="MFIGHYALGLGSKRLAPDVSLGALFLACQFADLLWPTLVMTGVE
                     SFTIRPGTTAVTPLDFQYYPYSHSLLALLVWGTALALIYRAVSGSALRGAVVLGALVV
                     SHWFLDVIVHRPDLPLAPGSALRLGIGLWNSIPATLAVEFGLYAAGVWLYCKATAARD
                     RIGSVGFWALAAFLALVEVANVFGPPPPSVGAVAVSAQAMWLLVAWGYWVDKHRQPVS
                     QLPSAS"
     gene            complement(1228..1530)
                     /locus_tag="VM713_02890"
     CDS             complement(1228..1530)
                     /locus_tag="VM713_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83241.1"
                     /translation="MEEIQPNVWRVTVGTITRVVRFRTDDMTFAPWDVTAADGQTLWS
                     ATSLASAFRWIQARTGYPVEALFAQALLESRGAESARPKGAEESTRTEGDQEALGS"
     gene            complement(1723..>2758)
                     /locus_tag="VM713_02895"
     CDS             complement(1723..>2758)
                     /locus_tag="VM713_02895"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HVH83242.1"
                     /translation="FMTMPEVVERIAGFAAAAAAPVRTQTTVTCVRRIDGGYHVNTDR
                     GEIRCRTVVLASGAANIPQVPTVQAAVPEAIGCVTPFEYRNPQQLPQGGVLVVGASAT
                     GVQLADEIQRSGRAVTIAVGEHVRLPRNYRGRDVLWWMEASGVWNQRYDEIDDIDRAR
                     RLPSPQLVGTPAHASLDLNILRAAGVEIVGRLATIRDGRALFSGGLANQFALADLKMN
                     RLLETFDEWARSQTPGVEAGAPERFETTRAPRSPRLSLDLRSGEIRALVWATGFRPDY
                     RWLDVPVLDRKGELRHDGGVVREAPGMYALGLPLLRRRKSTFIVGAEDDARDVIGHLA
                     RYLAAHSRSC"
BASE COUNT          438 a          928 c          894 g          498 t
ORIGIN      
        1 aatacgattg cggcggttgg cttcgagcag atcgagatcg agttcgaccc gagctggatg
       61 ggacacggcc ttctgccgac cgcacccgtc acgcggtgga ttggcggtcg agccgcggcg
      121 cgagcgcgct gcctcggcga ggcttgcgaa ggagagtcat ccgcaatgcg cctgcgcgca
      181 gcactgcaag gcttcctgca aagcgcgccc tcggagcccc accgggagtt tccgggctgg
      241 gttgacacgg tcgcgcgacg gctgagggaa aacaccacac tgcgagtcag cgatcttgcg
      301 cgcgaagtag gtcggcatcc atcttggctg ggatccgctt atcggcacgc ggccggggaa
      361 ggcatcaggg agactgccgc gagattccga gtagagcgcg cgacgtgcct cttgcgggag
      421 accgaccggc catatgcggc gatagccctt gacgcagggt tctgcgatca gagtcacatg
      481 aatcgaaccg tgcgccgcgt cctcggacgc tcaccctccg ccgtcaggca ggaccggcgg
      541 agctttcggc agagcgtggc ctgaggtcac cgtcttgttt atcggccatt acgcgctcgg
      601 tcttggctcg aagcggctcg cgcccgacgt ctcactcggc gccttgttcc tcgcctgcca
      661 attcgcggat ctgctctggc cgacactcgt gatgactggc gtcgaatcct tcacgatccg
      721 acccggcact accgcggtaa cgccgctcga ctttcagtac tacccctact cccacagcct
      781 gttggcgctg ctcgtatggg gcacagcgct cgcgctgatc tatcgcgcgg tgagcggttc
      841 cgccctccgc ggcgccgtcg tgctcggtgc actggtcgtc tctcactggt ttctcgatgt
      901 gatcgtgcac cgcccggatc tgcctctggc gcctggaagt gctctgcggc tcgggattgg
      961 tttgtggaat tcgattccgg caactctcgc tgtggagttc ggcctgtacg cggccggcgt
     1021 ctggctgtac tgcaaggcga ccgcagcgcg cgaccggatt ggatcagtgg gattttgggc
     1081 cctggcggcg tttctcgcgc tcgtggaggt cgcgaacgtt ttcggcccac cccctccaag
     1141 tgtgggcgcc gtcgccgttt cggcccaggc gatgtggctc ctcgtcgcgt ggggatactg
     1201 ggtcgataag catcgccagc cggtgagtca gctgccgagc gcctcttgat cgccttcagt
     1261 ccgcgtcgat tcctcagcgc ccttcgggcg cgctgactct gcgcccctgc tttccaaaag
     1321 cgcttgagcg aacaacgcct ccaccggata gccggtgcgc gcctggatcc accgaaaagc
     1381 cgacgcgagc gaggtagcgc tccagagtgt ctgcccatcc gcagccgtca cgtcccaggg
     1441 cgcgaaagtc atgtcatcgg tgcgaaagcg aacgacgcgc gtgatcgtcc cgacggtcac
     1501 ccgccatacg ttcggctgaa tttcttccac acggtaatcc atgggccctt ccccgaaacg
     1561 tccctgcaat cccaacccgc gactcaacaa ttattcttgt gctcgcgggc gttaggataa
     1621 gcctgttatc ttgcactgca actctattga acatatggcg ccgaaacaga acatgaagcg
     1681 gcctaccagg ccctaaccct ctaacagcgg cggcggtgtg agtcagcagc tgcgtgagtg
     1741 agctgcgaga tagcgcgcga gatgcccgat cacatcgcgc gcatcgtcct ccgccccaac
     1801 gatgaatgtc gacttgcgcc ggcgtaacag cgggagtccc agcgcgtaca tcccgggcgc
     1861 ctcgcgcacg acgccgccgt cgtggcgcag ctcgcccttg cggtcgagaa ccggtacgtc
     1921 gagccagcga tagtcaggac gaaacccggt cgcccagacg agcgcgcgga tctcgccgct
     1981 gcgcaagtcg agcgacaggc gtggcgagcg cggcgcgcgc gttgtctcga aacgctccgg
     2041 cgcccccgct tccacccctg gcgtctggct gcgcgcccac tcgtcgaagg tctcgagcaa
     2101 gcggttcatc ttcaggtcag cgagcgcgaa ctgattggcc agcccaccgg aaaagagcgc
     2161 gcggccgtcg cgtatggtcg ccagccgccc gacgatctcg actcccgcgg ctctgaggat
     2221 gttcagatcg agactcgcat gcgcgggtgt gcccacgagc tgcggcgatg gcagccgccg
     2281 ggcgcggtcg atgtcgtcga tctcgtcgta gcgctgattc cacacgcccg aggcttccat
     2341 ccaccagagc acgtcgcggc cgcggtagtt ccgtggcagg cgcacgtgct caccgaccgc
     2401 tatcgtcacg gctcgccccg agcgctggat ttcatcggcc agctgcacac cggtggccga
     2461 ggcgccgacg acgagcacgc cgccctgcgg cagctgctgc ggattgcggt attcgaaggg
     2521 cgtcacgcag ccgatggcct cgggtactgc ggcctgcacg gtcggtacct gggggatgtt
     2581 cgccgcgccg ctggcgagta ccacggtgcg gcaacggatc tcgccgcggt cggtgttgac
     2641 gtgatagccg ccgtcgatgc gccgcacgca ggtgacggtc gtctgggtgc gcacgggagc
     2701 cgcggccgcc gccgcgaatc ccgcgatgcg ctcgacgacc tcgggcatcg tcatgaag
//
LOCUS       DATLKL010000221         1577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_58907, whole genome shotgun sequence.
ACCESSION   DATLKL010000221 DATLKL010000000
VERSION     DATLKL010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58907"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VM713_02900"
     CDS             <1..1151
                     /locus_tag="VM713_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVH83243.1"
                     /translation="AEDVPAVSPAFTPAEAERRQLTVMFCDLVGSTPLSTRIDPEDLR
                     EIVGAYHRCVADTVGRFGGFVAKYMGDGVLIYFGYPEAHEDDAERAARAGLGVIDAVG
                     RLATQEPLNVRIGIATGLVVVGDLIGAGAAQERGVVGETPNLAARLQALARPGTLVIA
                     DSTRRQIGTLFEIEDLGPQPLAGFAEPQRAWRVAGESGVVSRFEALRSGTTPLVGRDE
                     ELDLLLRRWQQAKSGEGRVVLVSGEPGIGKSRLSAALSQRIESEPHTRLRYFCSPHDQ
                     DSALYPFIGQLERAAGFARDDTATMKLDKLEALLGGSPLPNPPPQAGEGRVGAGAEPG
                     DLSLIAECCRFPAASAFRRSTSARSARRSGHWRRYCGSSRHWHGGSRS"
     gene            1109..>1577
                     /locus_tag="VM713_02905"
     CDS             1109..>1577
                     /locus_tag="VM713_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83244.1"
                     /translation="MRQLQALARRQPVLMIFEDLHWIDPTSRELLDLTVEEITQLPVL
                     LVATYRPEFQPPWVGGSQVTVIALNRLGRNEGATLVHRLAGNLGALPPDVVDQIVERS
                     DGVPLFVEELTKAVVEAGADRGYASLAAVPASSLAVPATLNASLLGRLDRLGPS"
BASE COUNT          226 a          521 c          565 g          265 t
ORIGIN      
        1 cggccgagga cgtgccggct gtgtcccctg cgttcacccc tgccgaggcg gagcggcgcc
       61 agctgaccgt gatgttttgc gatctggtgg gctcgacgcc gctgtccacg cgcattgatc
      121 ccgaggatct gcgcgagatc gtcggtgcct atcatcgctg cgtcgccgac accgtcggcc
      181 gctttggcgg ttttgtcgcc aagtatatgg gcgacggggt gctgatttat ttcggctatc
      241 ccgaggcgca tgaggatgac gccgagcgcg cggcccgcgc tgggcttggc gtgatcgacg
      301 cggtcggccg gctcgccaca caagagccgt tgaacgtgcg gatcggcatc gccactggcc
      361 tcgtcgtggt cggcgatctg atcggcgcgg gtgcggcgca ggagcgcggc gtggtcggcg
      421 agacgccaaa tctcgccgcc cgcctgcagg cgctggcccg gccgggcacg ctcgttattg
      481 ccgacagcac ccggcgacag atcggtacgc tgttcgagat cgaggatctc ggcccccagc
      541 cgctcgccgg tttcgccgag ccccagcgtg cttggcgggt agccggcgag agcggggtcg
      601 tcagccgctt tgaggcattg cgttcgggga ccacaccact ggtcggccgc gacgaggagc
      661 tcgacctctt attgcggcgc tggcagcagg cgaagtccgg cgaggggcgg gtggtgctgg
      721 tctccggcga gccggggatc ggcaaatcgc gcctttccgc ggcgctgtcg caacgcatcg
      781 agagcgagcc gcacacccga ctacgctatt tctgctcacc gcacgatcag gacagcgcgc
      841 tctacccatt tatcggccag ctggaacgcg ccgcggggtt cgcccgcgac gacacggcga
      901 cgatgaaact ggacaagctg gaggcactgc tcggcggcag ccccctcccc aaccctcccc
      961 cgcaagcggg ggagggaagg gtgggagccg gggcggagcc gggcgacctc tcgttgatcg
     1021 ccgagtgctg tcgctttccg gcggcgagcg ctttccgccg ctcgacctca gcccgcagcg
     1081 caagaaggag cggacactgg cggcgctatt gcggcagctc caggcattgg cacggcggca
     1141 gccggtcttg atgatcttcg aagacctgca ttggatcgat ccgacctcgc gcgagctgct
     1201 cgaccttacg gtcgaagaga tcacacagct gccggtgctg ctggtcgcaa cctaccggcc
     1261 cgagttccaa ccgccctggg tcggagggtc gcaggtgacg gtgatcgcgc tgaaccggct
     1321 cggccgcaat gaaggagcga cgctcgtgca tcggctcgcc ggcaatctcg gggcattgcc
     1381 gccggatgtt gtcgaccaga tcgtcgagcg cagcgacggg gtgccgctct ttgtcgagga
     1441 attgaccaag gcggtggtcg aggccggggc cgaccgcggc tatgcgtcgt tagccgccgt
     1501 gccggcgtcg tcgctcgcgg tgccggccac gttgaacgcc tcgctgctgg ggcgcctcga
     1561 tcggctgggc ccctcgg
//
LOCUS       DATLKL010000222         2776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_160444, whole genome shotgun sequence.
ACCESSION   DATLKL010000222 DATLKL010000000
VERSION     DATLKL010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160444"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VM713_02910"
     CDS             <1..398
                     /locus_tag="VM713_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83245.1"
                     /translation="NAFALLHQLGDERLSTDTEGALLTRRHDGALVLALWNYREVGAS
                     AGTTRRVVLEVRHSGASTASVQSLDAQHGDVHGAYEQMGSPRNPTQSQLSQLRAAGAL
                     PAPVTRALEAGSLTLEIPPDGLTLVTIGP"
     gene            666..>2776
                     /locus_tag="VM713_02915"
     CDS             666..>2776
                     /locus_tag="VM713_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HVH83246.1"
                     /translation="MRKGFDNSVGWASSYRATVALAFATAALSLSLVRAAPAAQPMHF
                     TRLGADAGLAQGGVMAIAQDREGFLWLGTEDGLDRYDGYTLQHLIRQRGLAGTLPNNW
                     IAALAVGADGRLWVGTDGGGVAWRDPLTGQFVQIRPAPGAPSLAAEEKVRALHIDRNG
                     RLWVGTRAAGVAELDVTTGRVRRFQHSDADSATLSDDGVFAIAGARSGAIWVATHSGL
                     DRLDPVSGRVVRLGSRIAAALDAAGPVQMNALLEDQLGELWVGTSSGLAMIEASGERI
                     LAYRSHRDRPGSLPSDRVQALLEDDARRLWVGTAEGLALFDRRADRFDTYQHAAADPA
                     SLPDDYVISLYQDRGGLLWIGTKTKGVAKWNPRSWSFGHQLGDDPAGTAVTAFAEEGD
                     TLWLGTFGAGLSAIDRRTGAARHYRHKPGDPNSLPDDRVMAALVDHRGTLWVGTMTAG
                     LARLEPGLGAFRTYQPVASDAGSLPAPGVMSLLEDTHGNLWVGTYGGGLARFVRATDR
                     FQRYPSQPANDGLSSDRATALAEDPTGRLWVGTDGGGLNLLDPGSGHVVRFAHKHDDT
                     STLSANTVYSVHVDARGSVWVGTRGGGLDHVIGTAGQPDGLRFRNYSEADGLANDTIY
                     GIESDSAGRLWLSTNAGLSRFEPDSGTIRNFHQSHGLQGEEFNFGAHFRSANGELFFG
                     GPNGYNAFFPERLLYDDTPPPV"
BASE COUNT          459 a          901 c          954 g          462 t
ORIGIN      
        1 tcaacgcctt cgctctcctg catcagttgg gcgatgaacg cctgtcgacc gacacggagg
       61 gcgcgttatt gacgcgccgc cacgatggcg ctctggtgct cgcgctctgg aactaccggg
      121 aggtcggcgc gagcgcggga acgacccgga gggtcgtgct ggaggtcaga cacagtggcg
      181 cgagcacggc cagcgtccag tccctcgatg cccagcacgg ggacgtacat ggcgcctacg
      241 agcagatggg ctcgccgcgt aatccgacgc agagccaact ctcccaattg cgcgcggccg
      301 gcgcactgcc cgcgcccgtc acccgtgcgc tcgaggccgg ctctctgacg ctcgagattc
      361 cacccgatgg gctgacactc gtcacgatag gaccgtgaat gagctgcgtc cgccaaacgt
      421 gacccggctc acacttgcag gcttcccgcg tcacgcgcga cacgatcacc ctggagcccg
      481 ttcacagtac aggggtggcc atttcctcaa gaatacgcgg tggtcacacc tgttcggtcc
      541 cggggacgca gcatggcgcc ctctgggggg caaggtcgcg catcatgagt ttcccagcgc
      601 agactcaagg aacgatcgcc ggcaagcagc gagcgtgtcg ctgcgccttg cagaagggtg
      661 gcgtcgtgcg gaaggggttt gacaatagcg tcggttgggc cagcagctac cgcgcgacgg
      721 tggcgctggc tttcgcgacg gcagcgctga gcttgagcct ggtgcgggct gcgccggccg
      781 cgcaaccgat gcatttcacg cgcctggggg ccgatgcggg gctcgcgcag ggcggagtga
      841 tggccatcgc acaggaccgt gagggctttc tctggctcgg caccgaggac gggctggatc
      901 gctacgacgg ctacacgctg cagcatctga tccgccagcg cggattggcg gggactttac
      961 cgaacaactg gatcgccgcg ctcgcggtcg gcgccgatgg caggctctgg gttggcaccg
     1021 atggaggcgg cgtcgcctgg cgagatccgc tgacggggca attcgtccag atacggcctg
     1081 cgcccggcgc gccatcgcta gcggccgagg agaaggtgcg cgccttgcat atcgatcgca
     1141 acggccgtct ctgggtcggc acgcgcgcgg ccggagtcgc ggaactcgat gtcacaacgg
     1201 gtcgtgtgcg ccgtttccag catagcgacg cagacagcgc cactctgagc gatgacgggg
     1261 tttttgccat cgccggagcg cgtagcggtg ctatatgggt cgcgacgcat tcgggcctcg
     1321 atcggctcga tccggtgagt ggacgcgtcg tccgactcgg gtcgcgtatc gcggcggccc
     1381 tcgacgctgc cggcccggtg cagatgaacg ccctgttgga agatcagctc ggtgagctgt
     1441 gggtgggcac gagctccgga ctcgcgatga tcgaagcttc gggcgagcgg atcctggcgt
     1501 atcgaagcca tcgagataga ccggggagcc tccccagcga tcgagtccag gcactcctgg
     1561 aggatgacgc acggcgcctg tgggtcggca cggccgaggg acttgcgctc ttcgaccggc
     1621 gcgccgatcg attcgacacg taccagcatg cggccgcgga cccggcgagt ctcccggatg
     1681 actatgtcat ttccctgtat caggaccgtg ggggactgct gtggatcggc accaagacca
     1741 agggcgtggc caagtggaac ccgcgctcgt ggtcgttcgg acatcagctg ggggacgatc
     1801 cggcgggcac tgcggtgacc gcgttcgccg aggagggcga cacactgtgg cttggaacgt
     1861 ttggggcggg cttaagcgcg atcgaccggc gcacgggcgc agcgcgccac taccgacata
     1921 agccgggtga cccaaatagc ctccccgacg atcgtgtgat ggcggcgctg gtcgatcacc
     1981 gtgggaccct atgggtgggc acgatgaccg caggcctcgc gcgtctcgag ccgggactcg
     2041 gcgcgtttcg cacttaccag cccgtcgctt ctgacgcagg gtcgctgccc gcaccgggtg
     2101 tcatgtcgtt actggaggac actcacggca atctatgggt cggaacgtac ggcggtggcc
     2161 tggcgcgctt cgtacgcgcc accgaccggt tccagcgtta cccctcgcaa cccgccaacg
     2221 acggtctctc gagcgaccgg gccacggccc tggccgaaga cccgaccgga agactctggg
     2281 tcggtaccga cggcgggggc ctgaacctgc tcgatcccgg atcggggcac gtcgtgcgct
     2341 tcgcccataa gcacgatgac acgagcacgt tgagtgccaa cacggtttac tccgtacatg
     2401 tcgatgcacg cggcagcgtc tgggtgggta cgcgcggcgg cggcctcgat cacgtcatcg
     2461 gcacggcagg ccaacccgat ggtctgcgct tccgcaacta ctccgaggcc gacgggctcg
     2521 ccaacgacac catctacggc atcgagtcgg attcggccgg gcggctgtgg ctgagcacca
     2581 acgcgggtct gtcgcgcttc gaacccgaca gcggaaccat ccgcaacttt catcagagcc
     2641 acgggttgca gggggaggag ttcaatttcg gcgctcattt ccgcagcgcc aacggtgagc
     2701 tctttttcgg cggcccgaac ggttacaacg ccttcttccc cgagcggctt ctctacgacg
     2761 acactccacc gccggt
//
LOCUS       DATLKL010000223         1604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_92824, whole genome shotgun sequence.
ACCESSION   DATLKL010000223 DATLKL010000000
VERSION     DATLKL010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92824"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VM713_02920"
     CDS             <1..771
                     /locus_tag="VM713_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVH83247.1"
                     /translation="ERVFIATKFGFVRDPANPASHAIDARPARVREACEASLKRLKVA
                     YIDLYYLHRVDPQVPIEDTVGAMAELVRTGKVRYLGLSEVAPGTLERAHRVHPIAALQ
                     SEYSLWTRDPEDGVLEACQRLGVGFVPFSPLGRGFLTGRIRNPDDFAADDYRRQNPRF
                     QGENFTRNLRLVEKVQALAKEKGCTPGQLALPWVLAQGEYIVPIPGTRRVKNLEENLG
                     ALEVRLSPGELASIEAVFPVSAVAGARYTENMMRLIRG"
     gene            complement(806..1528)
                     /locus_tag="VM713_02925"
     CDS             complement(806..1528)
                     /locus_tag="VM713_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83248.1"
                     /translation="MAIRTLTIAARFRGPPASSNGGYFSGVVAGCAARTVTVRLIRPP
                     PLDTELAVEESADGTLRVLSGGEAIGEARPAELTLDAPPPPDYLEAVEASRHYAGFRY
                     HRFPSCFVCGTQRVRGDGMRVFAGPLPQRGLVAAPWVPDTSLDSGDGKVHAEFMSAAL
                     DCPGYYAVAPDDRMMLLAEFTAHVDRRVHVGESCTVVGWQIGSSGRKHEAGTALFDGK
                     GELCGRARALWIEPRDAPPPLG"
BASE COUNT          254 a          554 c          566 g          230 t
ORIGIN      
        1 gagcgggtct tcatcgcgac gaagttcggc ttcgtgcgcg atcccgccaa cccggcctcc
       61 cacgccatcg atgctcgtcc ggcccgcgtg cgcgaggcgt gcgaggcgag cctgaagcga
      121 ctgaaggtgg cgtacatcga cctctactat ctgcatcgtg tcgacccgca ggtgccgatc
      181 gaggacaccg tcggcgccat ggcggagctg gtgcgcacgg gcaaggtgcg ctatctgggg
      241 ctttccgagg tcgccccggg gactctcgag cgcgcgcacc gcgtgcaccc gatcgctgcg
      301 ctccagagcg agtattcgct gtggacgcgc gacccggagg atggcgtgct cgaggcctgc
      361 caacggctgg gagtcggctt cgtgcccttc agcccgctcg ggcgcggctt cctcaccggc
      421 cgcatccgca atccggatga cttcgccgcc gacgactacc ggcgccaaaa tccacgcttt
      481 cagggtgaga atttcacgcg caacctgcga ctggtcgaga aggtgcaggc gctggcgaaa
      541 gagaagggtt gcacccccgg gcagctcgcg ctcccctggg tgctggcgca gggtgagtac
      601 atcgtgccca tcccgggcac gcgccgggtg aagaatctcg aggagaatct cggcgcgctc
      661 gaggtgcggc tgtcgccggg cgagctcgcc tcgatcgagg cggtgtttcc cgtcagtgca
      721 gtggcgggtg cgcgctacac cgagaacatg atgcgtctga tccgcggctg aaggcacagt
      781 gcccgccgcc aaaggtggcg agcgactagc cgagcggcgg cggcgcatcg cgcggctcga
      841 tccacagcgc ccgcgcccgc ccgcacagct cccccttgcc atcgaagagc gcggtccctg
      901 cctcgtgctt gcgcccgctc gagccgatct gccaaccgac gaccgtgcag gattccccga
      961 cgtgcacccg ccggtcgacg tgcgcggtga attccgcgag cagcatcatc cggtcgtcgg
     1021 gcgccaccgc gtagtagccg gggcagtcga gcgctgccga catgaactcg gcgtgcacct
     1081 tgccgtcgcc gctgtcgagg gaggtatcgg ggacccaggg ggccgcgacc aggccgcgct
     1141 gcgggagtgg gccggcaaac acccgcatac catcgccgcg cacgcgctgc gtcccgcaca
     1201 caaaacagct cgggaagcgg tgatagcgaa agcccgcata gtgtcgcgag gcttcgactg
     1261 cctcgaggta gtcgggaggg ggcggcgcat ccagcgtgag ctccgccggc cgtgcctccc
     1321 cgatggcctc gccgccgctc agcacacgca gcgtgccgtc cgcggattcc tcgacggcga
     1381 gctcggtatc gagcggcggc ggccggatga gacgcacggt cacggtgcgc gcggcgcagc
     1441 cggcgaccac gccggagaag tagccgccgt tcgaggaggc cggcgggccg cggaaacgcg
     1501 ctgcgatggt gagggtgcgg atcgccacgg tgtacagaat cggcagttta cgtcctcagc
     1561 gcaaatcagc catagagccg ggccggccgg ctaaacgcgc cgcc
//
LOCUS       DATLKL010000224         1735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_171742, whole genome shotgun sequence.
ACCESSION   DATLKL010000224 DATLKL010000000
VERSION     DATLKL010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171742"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..782)
                     /locus_tag="VM713_02930"
     CDS             complement(126..782)
                     /locus_tag="VM713_02930"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HVH83249.1"
                     /translation="MANETTHRFIAVEGPIGVGKTSLARRLCASLSAQGVLEDATHNP
                     FLERFYRNPRAGALPAQLCFLLQRSQQLTSLKQADLFAPVRVADYLLEKDRLFARVTL
                     DEAEYALYEQLYARLDIQLPKPDLVVYLQAPVDVLLERIARRGVAYEQYIDRRYLERL
                     NEAYARFFHEFDTAPLLIVNAASIDPISNQRDYDELLAAIRRMSRGRLYYNPLRSRAM
                     "
     gene            complement(801..1289)
                     /gene="folK"
                     /locus_tag="VM713_02935"
     CDS             complement(801..1289)
                     /gene="folK"
                     /locus_tag="VM713_02935"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HVH83250.1"
                     /translation="MVSWRPAYIGVGSNLEEPRAQVLKACRELAQLPLTRLELTSPLY
                     RSRPMGPIAQPDFVNAVAGLLTQLEAPALLAELRALEAALGRPATHQHWGPRVIDLDL
                     LSYGRERRAGPELTLPHPGIPERNFVLYPLADIAPDLDIPGLGRVAELAGRLTTEGLA
                     RL"
     gene            complement(1303..>1735)
                     /locus_tag="VM713_02940"
     CDS             complement(1303..>1735)
                     /locus_tag="VM713_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83251.1"
                     /translation="IAAIIETTPPERWHELATILDACDQAIREAQTHVAIPKRFALGV
                     REMFALQPRLEHPRGRRALRVLEHPRFRAGYDLLLLRAEFGLAPAEMATWWTRLQEVP
                     DEERNRMVDALTGAGARGAPAARRGGRRRRRRPKARATAPS"
BASE COUNT          275 a          576 c          617 g          267 t
ORIGIN      
        1 gtcataacag gtgaggctgg cgatcttctc gccgtcagcc ttcatgcggc cgagcgtcga
       61 gagcgtgacc ggcgagcggg tggcgtcgcg ttgcagatgc gtatacatag cggaaccgag
      121 tgtacttaca tcgcgcggct gcgcaacgga ttgtagtaaa ggcgcccgcg actcatgcgt
      181 ctgatggccg ccagcagctc gtcgtagtcg cgctggttgc tgatcgggtc aatggacgcg
      241 gcgttcacga tgaggagcgg cgccgtgtcg aattcgtgaa agaagcgcgc gtaggcctcg
      301 ttgagccgct cgagatagcg gcgatcgatg tactgctcgt aagcgacgcc gcgccgtgcg
      361 atgcgctcga gcagcacgtc gacgggagcc tgcaggtaca ccaccagatc gggcttgggc
      421 aactggatgt cgagccgcgc gtagagctgc tcgtagagcg cgtactcggc ctcatcgagc
      481 gtcacgcgtg cgaacagccg atccttctcc agcaggtaat cagcaacccg caccggagca
      541 aagaggtccg cctgcttgag tgaggtcagc tgctgcgaac gctgcaggag gaagcacagc
      601 tgcgccggca gcgcgccggc gcgcgggttc cggtagaaac gctcgaggaa cgggttatgc
      661 gtcgcatcct ccagcacgcc ctgcgcggac aggctggcgc acagacggcg ggcgaggctc
      721 gtcttgccga cgccgattgg accttcgacc gcgatgaagc gatgggttgt ctcgttcgcc
      781 atgtgcgcaa ctcgcgcgtc tcagagccgc gccagtccct cagtcgtgag cctgcccgcg
      841 agctcggcga cgcgcccaag cccggggata tcgagatccg gggcaatgtc cgccagagga
      901 tacagaacga agttgcgctc cggtatcccg gggtgcggca gtgtgagctc ggggccggcg
      961 cggcgctcgc gcccgtagga gagcaggtcg aggtcgatca cccgtggacc ccagtgctgg
     1021 tgcgtggccg gacggccgag cgcagcctcg agcgcacgta actccgcgag cagtgccggc
     1081 gcttcgagct gggtgaggag gccagcgacc gcgttcacga agtccggctg tgcgatcggc
     1141 cccatggggc gcgagcgata gaggggcgag gtcaactcca ggcgcgtcag cggcagttgc
     1201 gccagctccc tgcaggcctt gagcacctgc gcacgcggtt cctcgaggtt gctgcccacg
     1261 ccgatatatg cgggccgcca cgacaccatg tgcgccggtc catcaggacg gggcggtggc
     1321 cctggccttg gggcgtcggc gccgccggcg cccgccgcgc cgcgctgcag gcgccccgcg
     1381 cgcgccggcg cccgtgagcg cgtccaccat ccgattgcgc tcctcgtccg gcacctcctg
     1441 cagccgtgtc caccaggtcg ccatctccgc cggtgccagc ccgaactcgg cccgcagcag
     1501 caacaggtcg tagccggcgc ggaagcgcgg atgctccagc acgcgcagcg cccgccggcc
     1561 gcgcgggtgc tcgagccgcg gctgcagtgc gaacatctcg cgcacgccga gggcaaagcg
     1621 cttcgggatc gccacgtgcg tctgggcctc gcggatcgcc tgatcgcagg catccagtat
     1681 ggtcgcgagc tcgtgccagc gctccggcgg cgtcgtctcg atgattgcgg ctatg
//
LOCUS       DATLKL010000225         1879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_58969, whole genome shotgun sequence.
ACCESSION   DATLKL010000225 DATLKL010000000
VERSION     DATLKL010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58969"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /gene="xseA"
                     /locus_tag="VM713_02945"
     CDS             complement(<1..569)
                     /gene="xseA"
                     /locus_tag="VM713_02945"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HVH83252.1"
                     /translation="MALRIEEPSVTPPRDVYTVSRLNREVRSVLERGLGVIWVQGELS
                     NFSQPSSGHWYFSLKDRGAQLRCAMFRMQNALVGFTPQDGAQLLLRGRISVYEARGEY
                     QLIVEHLEEAGVGALKRAFELLKTRLAAEGLFAAERKRPLPRFPRRIGVITSPSGAAL
                     HDILHILARRFPPAAVLIYPAPVQGAAAVP"
     gene            623..>1879
                     /gene="guaB"
                     /locus_tag="VM713_02950"
     CDS             623..>1879
                     /gene="guaB"
                     /locus_tag="VM713_02950"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003295955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HVH83253.1"
                     /translation="MRILEEALTFDDVLLVPAYSEILPREVDLSTRVTRRIRLNVPLM
                     SAAMDTVTEARLAITIAQEGGIGVIHKSMTIEAQAREVGRVKKFESGVIKDPITVSPN
                     MSIREVLDLTRSRGISGVPVVVGRKAVGIVTHRDLRFEDKLDAPVSSVMTPKERLVTV
                     REDAAKEQVLALLHKHRIEKLLVVNGDHELVGMITVKDFQKATEFPRACKDERGRLRV
                     GAAVGTTPDTLERVAALREAGVDLVVVDTAHGHARGVIETVGRIKAKWADAQVIAGNI
                     ATAEAARALKDAGADAVKVGIGPGSICTTRIVAGVGVPQISAVVNVAEALRDADVPVI
                     SDGGIRFSGDIAKALAAGAHAVMIGGLFAGTEESPGEVELYQGASYKSYRGMGSLGAM
                     GERHGSADRYFQDAASELEKLVPEGVE"
BASE COUNT          308 a          644 c          652 g          275 t
ORIGIN      
        1 ggtaccgcgg cggcgccctg caccggggcc gggtagatga gcaccgccgc cggcggaaag
       61 cgccgcgcga ggatgtgcag aatgtcgtgc agcgccgcgc ccgagggcga ggtgatcacc
      121 ccgatgcgcc gcggaaagcg cggcagcggg cgcttgcgct cggcggcgaa caggccctcg
      181 gcggcgagcc gggtcttcag cagctcgaag gcgcgcttga gggcgccgac ccccgcctcc
      241 tcgaggtgct cgacgatcag ctggtactcg ccacgcgcct cgtagacgct gatgcgcccg
      301 cgcagcagca attgtgcgcc gtcctggggc gtgaacccga ccagcgcgtt ctgcatgcgg
      361 aacatggcgc agcgcagctg cgcaccgcga tccttgagcg agaagtacca gtggccggaa
      421 gatggctggg agaagttcga cagctcccct tgcacccaga tcacgcccaa gccccgctcg
      481 aggaccgagc gcacctcgcg gttgaggcgc gagaccgtgt agacatcccg cggcggggtg
      541 acgcttggtt cctcaatcct cagggccatg cggcacctta gcacgaggtt taccgccgtc
      601 gccccgccgc ctataattac gcatgcgcat cctcgaagaa gctctcacct tcgacgacgt
      661 cctgctggtt ccggcctact cggaaattct gccacgcgag gtcgatctct ccacccgcgt
      721 cacgcgccgc atccgcctga atgtgccgct catgtccgcg gccatggaca ccgtgaccga
      781 ggcgcgcctc gccatcacca tcgcgcagga aggcggcatc ggggtcatcc acaagagcat
      841 gaccatcgag gcgcaggcgc gtgaagtcgg gcgggtgaag aagttcgaga gcggcgtcat
      901 caaggacccg attaccgtct cccccaacat gtcgatccgc gaggtgctcg acctcacgcg
      961 ctcgcgcggc atctccggcg tcccggtggt agtcggccgc aaggcggtcg gcatcgtcac
     1021 ccaccgcgac ctgcgcttcg aggacaagct cgacgcgccg gtgtcctccg tcatgacgcc
     1081 gaaggagcgc ctcgtcacgg tgcgcgaaga tgctgccaag gaacaggtgc tcgcgctgct
     1141 gcacaagcac cgcatcgaga agctgctggt ggtgaacggc gaccacgagc tggtcggcat
     1201 gatcacggtc aaggattttc agaaggccac cgagttcccg cgcgcctgca aggacgagcg
     1261 cggtcggctg cgggtcggcg cggcggtcgg caccacgccc gacacgctcg agcgcgtcgc
     1321 ggcattgcgc gaggcggggg tcgatctggt ggtggtcgac accgcgcacg gacacgcgcg
     1381 tggcgtcatc gagacggtcg ggcgcatcaa ggcgaaatgg gccgatgccc aggtgatcgc
     1441 cggcaacatc gcgaccgccg aggccgcccg tgccctgaag gatgccggcg ctgacgcggt
     1501 caaggtcggc atcggcccgg gctcgatctg caccacgcgc atcgtcgccg gtgtcggcgt
     1561 gccgcagatc tcggcggtcg tgaacgtcgc cgaggcgctg cgcgatgccg acgtgccggt
     1621 gatctccgat ggcggcatcc gcttctccgg cgacatcgcc aaggcactcg cggccggtgc
     1681 gcatgcggtc atgatcggcg gcctgttcgc gggcaccgag gagtcccccg gcgaagtcga
     1741 gctctaccag ggcgcctcct acaagtccta ccgcggcatg ggttcgctcg gggcgatggg
     1801 tgagcgtcac ggctccgccg accgctactt ccaggacgcg gcgtcggagc tggagaagct
     1861 cgtgcccgag ggcgtcgag
//
LOCUS       DATLKL010000226         3735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_149360, whole genome shotgun sequence.
ACCESSION   DATLKL010000226 DATLKL010000000
VERSION     DATLKL010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149360"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="VM713_02955"
     CDS             <1..1495
                     /locus_tag="VM713_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVH83254.1"
                     /translation="LLADPVHQALVSGAPVTLGRRALLLSRSNAGERSIELSASPVRS
                     EAKEVVGAALLLHDVTELRGLARQMSYQATHDALTGLVNRREFERRLEEAIDSGHRGD
                     GQHVLCYLDLDRFKLVNDTSGHLAGDSMLREVAKLLRDAVRDSDTVARLGGDEFGMLL
                     IGCPLEKARQIADDVCRSVGDYRFVWKDRIFNIGVSVGPVEISRESGKLEELLAAADT
                     ACYVAKKQGSGRVAVYSARDEALARHSGEIQWLQRLQGALKENRFQLYHQVIVPTHDS
                     SGGPAMEVLIRLRDENGQELAPAEFMRAAERYRLMGLVDRWVVQTTLAAVGRGALPVP
                     PDRSVAINISGQTLGDLQFLEFVVECLDSTGVAPAQVCFEITENAVVANLEHARRFVG
                     VLHGMGCQFALDDFGSGVGSFSNLKNLPLDYLKIDGSFIRNLARDTVNQAMVTAMIKL
                     ARTLNFKVIAEQVEDSSAVEAARRMGVDYLQGFAIGRPQPLALAVAA"
     gene            complement(1500..3551)
                     /locus_tag="VM713_02960"
     CDS             complement(1500..3551)
                     /locus_tag="VM713_02960"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001951.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HVH83255.1"
                     /translation="MDLSMWPWLAIGAGVLAVLYGVFSMAAILRLPAGNARMQEIAAA
                     IQAGAQAYLNRQYSTILIVGLVLLVIIGFSPLGWATAGGFAVGAVLSGLTGYIGMNIS
                     VRANVRTAQAATRGLNAALTVAFRGGAITGMLVVGLALLGVAGYYTVLRQLGVSAEGA
                     LHALVGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNV
                     GDNVGDCAGMAADLFETYAVTIVATMLLGGLLFARRGPDAAYASVIYPLALGAASIVS
                     SIVGTFFVSTREGGKIMNALYKGVIVSGVVSAVAFYFITRAIMPAHAAELFGCTLIGL
                     GLTAAMIMITEYYTSTEYSPVRKIAAASQTGHATNIIAGLGVSMKATALPVVAVCLAI
                     WGSYTLSGLYGIAIAATAMLSMTGMIVALDAYGPITDNAGGIAEMAHLPKEVRNITDP
                     LDAVGNTTKAVTKGYAIGSAGLAALVLFADYTRNLEAAGKAASFTLYDPAVIIGLFIG
                     GMVPYLFGAMTLEAVGRAAGSVVNEVRRQFREIKGIMSGAEKPDYSRAVDLLTRAAIR
                     EMIVPSLLPIVMPIALVLVTNTLMRDGAGVRALGGLLIGTIVTGLFVAISMTTGGGAW
                     DNAKKYIEEGHFGGKGSEAHKAAVTGDTVGDPYKDTAGPAINPLIKIINIVALLLVPV
                     L"
BASE COUNT          617 a         1230 c         1327 g          561 t
ORIGIN      
        1 gctgctggcc gacccggtgc accaggccct cgtgagcggg gctccggtga cgctcggccg
       61 gcgcgcgctg ctgctgtcac gcagcaacgc cggcgagcgc tcgatcgagc tgtcggcatc
      121 gccggtgcgc agtgaagcca aggaggtggt cggcgcggcg ctgctgctgc acgacgtcac
      181 cgagctgcgc gggctcgcgc gccagatgtc ctaccaggcg acccacgatg cgctcacggg
      241 cctcgtcaac cgccgcgagt tcgagcggcg cctcgaggag gcgatcgaca gcggtcatcg
      301 cggcgacggc cagcacgtgc tctgctatct cgatctcgac cgcttcaagc tggtcaacga
      361 caccagcggc cacctggccg gcgacagcat gctgcgcgag gtcgcgaagc tgctgcgcga
      421 tgcggtgcgc gacagcgaca cggtggcccg cctcggcggc gacgagttcg gcatgctgct
      481 catcggctgc ccgctggaga aggcacggca gatcgccgac gacgtctgcc gttcggtcgg
      541 cgactaccgc ttcgtgtgga aggaccgcat cttcaacatc ggggtatcgg tcgggccggt
      601 ggagatctcg cgtgagagcg gcaagctgga ggagctgctg gccgcggccg ataccgcctg
      661 ctacgtcgcc aagaagcagg gcagcggccg ggttgcggtc tactcggcgc gcgacgaggc
      721 cctggcgcgc cacagcgggg agattcagtg gctgcagcgg ctgcagggcg cgctcaagga
      781 gaaccgcttc cagctctatc accaggtcat cgtgccgacg cacgacagca gcggcggacc
      841 ggcgatggag gtgctgatac ggctgcgcga cgagaatggc caggagctgg cgccggcgga
      901 gttcatgcgc gccgccgagc gctaccggct catgggcctg gtggatcgct gggtggtaca
      961 gaccacgctc gcggcggtcg gccgcggcgc cctgccggtg ccacccgacc ggagcgtggc
     1021 gatcaacatc tccgggcaga cgctcggcga cctgcagttc ctcgagttcg tggtcgagtg
     1081 cctcgacagc accggcgtcg cgccggcaca ggtctgcttc gagatcaccg agaacgcggt
     1141 ggttgccaac ctcgagcatg cgcggcgttt cgtcggcgtg ttgcacggca tggggtgcca
     1201 gttcgcgctc gacgacttcg gctccggcgt cggctccttc tccaatctga agaacctgcc
     1261 gctcgactac ctcaagatcg acggctcctt catccgcaac ctcgcgcgcg acaccgtcaa
     1321 tcaggcgatg gtgaccgcaa tgatcaagct ggcacgtacg ctcaacttca aggtgattgc
     1381 cgagcaggtc gaggacagca gcgcggtcga ggcggcacgg cgcatggggg tcgattacct
     1441 gcagggcttt gccatcggca ggccgcagcc gctcgcgctc gccgtcgcgg cgtagggcgc
     1501 tatagcaccg gcaccagcag cagcgcgacg atgttgatga tcttgatcag gggattgatc
     1561 gcggggccgg cggtgtcctt gtagggatcg cccacggtgt cgccggtcac cgcggccttg
     1621 tgggcttccg accccttgcc gccgaagtgg ccttcctcga tgtatttctt ggcgttgtcc
     1681 caggcgccgc cgccggtcgt catggagatg gcgacgaaga gacccgtgac gatggtgccg
     1741 atcagcaggc caccgagtgc gcgcacgccg gcgccgtcac gcatcagcgt gttggtcacc
     1801 agcaccagcg cgatcggcat cacgatcggc agcagcgagg gcacgatcat ctcgcggatg
     1861 gcggccctgg tgagcaggtc gaccgcgcgc gaatagtcgg gcttctcggc gccggacatg
     1921 atgcccttga tctcgcggaa ctgccgccgc acctcgttca cgaccgagcc ggcggcgcgg
     1981 cccacggcct ccagggtcat ggcgccgaac agataaggca ccatgccgcc gatgaacagg
     2041 ccgatgatca ccgcggggtc gtacagcgtg aagctcgcgg ccttgccggc cgcctcgagg
     2101 ttgcgggtgt agtcggcgaa cagcaccagc gcggcgagtc ccgccgagcc gatcgcatag
     2161 cccttggtga ccgccttggt ggtgttgccg accgcgtcca gcggatcggt gatgttgcgc
     2221 acttccttcg gcagatgcgc catctcggcg atgccgcccg cgttgtcggt gatcgggccg
     2281 taggcgtcga gggccacgat catcccggtc atcgacagca tggcggtcgc ggcgatggcg
     2341 atgccgtaga gcccgctgag cgtatacgaa ccccagatgg cgaggcacac cgccaccacc
     2401 ggcagcgcgg tcgccttcat cgagacgccg aggccggcga tgatgttggt ggcgtggccg
     2461 gtctgggacg cggcggctat cttgcgcacc gggctgtact cggtggaggt gtagtactcg
     2521 gtgatcatga tcatggccgc cgtcagcccc aggccgatga gcgtgcagcc gaagagctcg
     2581 gcggcgtgcg ccggcatgat cgcgcgggtg atgaaataga aggccaccgc cgagaccacg
     2641 ccggagacga tcacgccctt gtagagtgcg ttcatgatct tgccgccctc gcgggtcgag
     2701 acgaagaatg tcccgacgat cgaggagacg atcgaagcgg cgccgagcgc gagtgggtag
     2761 atcaccgaag cgtacgcggc gtcgggcccg cgacgtgcga acagcagccc gccgagcagc
     2821 atcgtggcga cgatcgtgac cgcataggtc tcgaagagat ccgcggccat gccggcgcag
     2881 tcaccgacgt tgtcgccgac gttgtcggcg atcaccgcgg ggttgcgcgg gtcgtcctcg
     2941 gggatgcccg cctcgacctt gccgacgagg tcggcgccga cgtcggcgcc cttggtgaag
     3001 atgccgccgc cgaggcgcgc gaagatcgag atgagcgagc cgccgaaagc gagtccgacc
     3061 agcgcgtgca gcgcgccctc ggcgctcact ccgagctgcc gcagcacggt gtaatagccg
     3121 gcgacgccga gcagcgcgag ccccacgacc agcatcccgg tgatggcgcc gccgcggaag
     3181 gcgaccgtaa gcgcggcatt gagtccacgt gtggcggcct gcgcggtgcg cacgttggcg
     3241 cgcacggaga tgttcatgcc gatgtagccg gtgagccccg agagcacggc gcccaccgcg
     3301 aagccgccgg cggtcgccca gccgaggggc gagaagccga tgatcaccaa gagcacgagg
     3361 ccgacgatga ggatggtgct gtactggcgg ttgaggtacg cctgggcgcc ggcctggatg
     3421 gccgccgcga tctcctgcat gcgcgcattg cccgccggca ggcgcaggat cgccgccatg
     3481 gagaacacgc catagagcac ggcgagcacg ccggcgccga ttgcgagcca cggccacatg
     3541 ctgagatcca tcgagaaacc tcccccaaaa aaggccgcga actatagcac ggcgagcctt
     3601 ggccaccgcc ttggccgcgc ccggcaccgc aatgcagcaa gctagacttt gaattcgtcc
     3661 ttgagcttgc gctgcacggc gacgccgcgg atgtgcgcgt agtcgggcag gccgttgcgg
     3721 taggggagat aagcc
//
LOCUS       DATLKL010000227         2664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_115456, whole genome shotgun sequence.
ACCESSION   DATLKL010000227 DATLKL010000000
VERSION     DATLKL010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115456"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /gene="prpD"
                     /locus_tag="VM713_02965"
                     /gene_synonym="mmgE"
     CDS             <1..579
                     /gene="prpD"
                     /locus_tag="VM713_02965"
                     /gene_synonym="mmgE"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="functions in propionate metabolism; involved in
                     isomerization of (2S,3S)-methylcitrate to
                     (2R,3S)-methylisocitrate; also encodes minor aconitase or
                     dehydratase activity; aconitase C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HVH83256.1"
                     /translation="HAQTAVEAAMTLHPAVRDRIADIERIVIETQEPGVRIIDKTGPL
                     ANPADRDHCIQYVVAVPLIFGRLTAADYEDAVASDPRIDALRDRMQVRENPKFTEEYY
                     AADKRYIGNAVQVFFRDGSHTERVQVDFPIGHRKRRAEGMPVLVRKFQAAVDANFPPK
                     QAERIKAMFAKPAALDALPVNEFVAAMVTNGA"
     gene            complement(576..2111)
                     /locus_tag="VM713_02970"
     CDS             complement(576..2111)
                     /locus_tag="VM713_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HVH83257.1"
                     /translation="MRFVSLVSKPLVIAMLGAATVAVPVGALYFSGAAHASASTATPS
                     AAAPASAPAPASAAAPAAANPGVALPDFSGMVQKYGPAVVNITVTTKLSASWQGDEGD
                     DSGDNGNGNGNEDPFGPNSPFAPFFRGFGFRSPHPQPMHGEGSGFLIRSDGLIMTNAH
                     VVKGASEVTVRLTDRREYPAKVVGIDERSDIALVKISAKNLPTVKLGDSRTLKVGEWV
                     LAIGAPFGFDNSATAGIVSAKNRPLNGDAVPFIQTDVPINPGNSGGPLFNMRGEVVGV
                     NSQIYSRSGGYMGVSFSIPIDVAMKVGNQLETSGHVTRGKLGVVIQNVTQDLADSFGL
                     PQPEGALVSSVEKGGPAEHAGIEAGDVILKLNGEQINSTTELSEKIAELGPGASADLE
                     LWHNHAARNMSVKLGTLEEHRSARNQESPHEGGKLGLAVRPLTGEEQRQGNVKGGLLV
                     EQASGPAAEAGIQPGDIVLSANGAPVSSAAALKSAVENSKGHVALLIQRGDARIFVPV
                     RVG"
     gene            complement(2271..>2664)
                     /locus_tag="VM713_02975"
     CDS             complement(2271..>2664)
                     /locus_tag="VM713_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVH83258.1"
                     /translation="DRAHITMHSQTESLSEQDMADAAAYLAGKPLAASGKPTGAVPQA
                     AQLCVSCHGQDGVAIAPIYPSLAGQHEDYIVRALNEYKHGGRKNPVMKGFAANLKDED
                     IAQIAAYFSRLTPSLGTEPRPYTRLSAD"
BASE COUNT          415 a          883 c          926 g          440 t
ORIGIN      
        1 cacgcgcaga ccgcggtcga ggcggcgatg acgctccacc cggcggtgcg cgaccgcatc
       61 gccgacatcg agcgcatcgt catcgagacg caggagccgg gcgtacgcat catcgacaag
      121 accgggccgc tcgccaaccc cgccgaccgc gaccactgca tccagtacgt ggtggcggtg
      181 ccgctcatct tcggccggct taccgccgcg gactacgagg acgcggtcgc gtcagatccg
      241 cgtatcgacg cgctacgcga ccggatgcag gtgcgcgaga acccgaaatt caccgaagag
      301 tattacgccg ccgacaagcg ctacatcggc aacgccgtgc aggtgttctt ccgcgacggc
      361 agccacaccg agcgcgtgca ggtcgatttc cccatcggcc accgcaagcg tcgcgccgag
      421 gggatgccgg tgctggtcag gaaattccag gccgccgtgg atgccaattt tccaccgaag
      481 caggccgagc gcatcaaggc gatgttcgcc aaaccggcag cgctcgatgc gctgccggtc
      541 aacgagttcg tcgccgcgat ggtgacaaac ggggcttaac ccacccgcac cggcacgaag
      601 atgcgcgcat cgccacgctg gatgagcagc gcgacgtggc ccttggagtt ctcgaccgcg
      661 ctcttcagcg cggcggcgct cgatacgggt gcgccgttgg ccgagagcac gatgtcacca
      721 ggctggatgc cggcctcggc cgcgggtccc gaagcctgct cgaccaggag gccgcctttc
      781 acattgccct gccgctgctc ctcgcccgtg agcgggcgca cggcgagccc gagcttgccg
      841 ccttcgtgcg ggctctcctg gttgcgggca ctgcgatgct cctccagtgt gccgagcttc
      901 acgctcatgt tgcgcgcggc gtggttgtgc cagagctcga gatcggcgct ggcgccggga
      961 ccgagctccg cgatcttctc cgacagctcg gtggtgctgt tgatctgctc gccgttgagc
     1021 ttgaggatga cgtcgcccgc ctcgatgccg gcgtgctcgg ccggtccgcc cttctcgacg
     1081 ctcgagacca gtgcgccctc gggctgcggc aggccgaacg agtcggcgag gtcctgggtg
     1141 acgttctgga tcaccacccc gagcttgccg cgggtgacgt gaccgctggt ctcgagctga
     1201 ttgccgacct tcatggcgac atcgatgggg atcgagaacg acacgcccat gtagccgccg
     1261 ctgcggctgt agatctgcga gttgacgccg accacctcgc cgcgcatgtt gaagagcggc
     1321 ccgcctgagt tacccggatt gatcggcacg tccgtctgaa tgaaggggac ggcgtcgccg
     1381 ttcagcggcc ggttcttggc gctcacgatg ccggcggtgg cgctgttgtc gaagccgaac
     1441 ggcgccccga tcgcgagcac ccattcgccg accttgagcg tgcgcgagtc gccgagcttc
     1501 accgtgggca gattcttggc gctgatcttc accagcgcga tgtcggaccg ctcgtcgatg
     1561 ccgacgacct tggcggggta ttcgcgccgg tcagtgaggc gcaccgtcac ctcgctcgcg
     1621 cccttcacga cgtgcgcatt ggtcatgatc aacccgtcgg agcggatgag gaagcccgag
     1681 ccctcgccgt gcatcggctg cgggtgcggg gaccggaagc cgaagccgcg gaagaacggc
     1741 gcgaacggac tgttcgggcc gaacgggtct tcgttgccgt tgccgttacc gttgtcgccg
     1801 ctgtcatccc cctcatcgcc ctgccaggac gccgacaact tggtggtcac cgtgatgttc
     1861 accaccgccg gaccgtattt ctgcaccatg ccgctgaagt caggcagcgc gacgccgggg
     1921 ttagcggcgg ccggggcggc tgcgctcgcg ggggcaggcg cgctcgccgg tgcggcggcg
     1981 cttggcgtcg cggtgctcgc ggacgcatgc gcggcacccg agaaatacag tgccccgacc
     2041 ggcacggcga cggtcgccgc tccgagcatc gcgatgacga gcggcttcga gaccagggaa
     2101 acaaaacgca tggacggctc ctcgaaaagg aaaaactgct ctggcgggga acgatagaag
     2161 ggccggctta acggagtctt aaggcccttc attcgggggc ccgcacgggc agcggaaagc
     2221 tttgaaatgc cgggctcggg gaaagttccc cgctccagcc gcagcccgct tcagtcggcg
     2281 ctgaggcgcg tgtaggggcg gggctcggtg ccgagcgagg gggtgaggcg agagaagtaa
     2341 gccgcgatct gcgcaatgtc ctcgtccttg agattagcgg cgaagccctt catgaccggg
     2401 ttcttgcgcc cgccgtgctt gtactcgttg agcgcccgga cgatgtagtc ctcgtgctgt
     2461 ccggcgagcg aggggtagat cggcgctatc gcgacgccgt cctggccgtg gcaggagacg
     2521 cacagctgtg ccgcctgcgg cacggcgccg gtcggcttgc cgctcgcggc gaggggcttg
     2581 ccggcgaggt aggcggcggc gtccgccatg tcctgctcgg agagcgactc ggtctgcgaa
     2641 tgcatggtga tgtgcgcgcg atcg
//
LOCUS       DATLKL010000228         3631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_36553, whole genome shotgun sequence.
ACCESSION   DATLKL010000228 DATLKL010000000
VERSION     DATLKL010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36553"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VM713_02980"
     CDS             <1..867
                     /locus_tag="VM713_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HVH83259.1"
                     /translation="RAGPLDTMVALARRELWEHRSLWIAPLVVEGLLAISLLLGRINM
                     DLPEGVLTAQQRIAIFTIVQWALAQPLFIVTAICVAFYLLDCLYAERKDRSILFWKSL
                     PLSDELTVASKFLVAAVVVPLGVFLLAAVAGLVFAAIIAVRVPGGLTWSTYEWLRTEL
                     VLLLEVILGVLWYSPIAGALLLFSAWIRRNPILWASLVPVVAPVVERVALGTHYIWNF
                     EVYRTNGIWHKLTLGPDVFGSFHHLPPVGVVLDTFNIRGAFTDIDLWSGVIAAIAMAY
                     AAARVRRYRDDT"
     gene            944..2101
                     /locus_tag="VM713_02985"
     CDS             944..2101
                     /locus_tag="VM713_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVH83260.1"
                     /translation="MSATDSTAEAVEVSRTAEGLELRLTGEWRLPEVAALEAALAAVD
                     VATVARIRIAAAGITRLDLSGAWALFAFVRRARRAGAEVGFRGSPPDELRLVQETLAS
                     QALAEPEASATAAAPAAPGGRALNAIGRYVVRSSHDLLGGLAFLGRILITCAANLAHP
                     RRLRPISIVRHVYETGITAMPIVALIAFLISVIIAYMSAQQLRGLGAEIYVVDLVTIG
                     VLRELGVLLTSIIVAGRSGSAFAAELGSMKLNEEVDALIATGADPFELLVVPRVLGLV
                     IALPLLAIVADLIGLAGGALLCRYLLDMPLTQYVNRVSESISPTTFWVGIIKAPVFAL
                     LIALAGCYRGMQVRGSARVLGHMVTVAVVQAIFMVILADALFAVFFMKLKI"
     gene            2101..2868
                     /locus_tag="VM713_02990"
     CDS             2101..2868
                     /locus_tag="VM713_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVH83261.1"
                     /translation="MEPVVEVRGLVNRFDRQVVHDGLDMRVDEDEVFGIVGGSGAGKS
                     VLLRTILGLQRPAAGTVRILGSDITRLTEEQLRPVKARYGVAFQEGALYTGLTVLQNV
                     QLPMIEHLRLPQDALEGLALLKIRLVGLPADAADKYPSQLSGGMVKRAAVARALALDP
                     ELLFLDEPTSGLDPVSAAAFDDLVVYLQRHLRLTIVMITHDLDSIFRTCNRVGVIVDK
                     RMESDTLERIVEHPNPWIQAYFHGERARARLGAGHGA"
     gene            2858..>3631
                     /locus_tag="VM713_02995"
     CDS             2858..>3631
                     /locus_tag="VM713_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HVH83262.1"
                     /translation="MEREANYAAVGAFVLLVTLGGALFVYWYSDTREHRDYNRYEIYF
                     TGSVSGLERGAAVRYLGVPVGRVNDLRIDPRDSSRAEVIVDIGSSAPVSEHTVAELNL
                     QGVTGLLYIDLSEDRSGKRLAPPVASLKYPVIRSAPSRFDVFLAGLPELVAAVGNLVK
                     RADRLLSDENIAAVTATVANLDNASSSLPRTVNDLNSLISELRGASAELAASAHLAHG
                     ILEAVDPDVRAAAQRLRAIADSLADATRQLDAAITENRQD"
BASE COUNT          506 a         1282 c         1205 g          638 t
ORIGIN      
        1 cgcgccggcc cgctcgacac catggtcgcg ctcgcgcgcc gtgagctctg ggagcaccgc
       61 tcgctgtgga tcgcgccgct ggtggtcgag ggactgctgg ccatctcgct cctcctcggc
      121 cgcatcaaca tggacctccc cgagggggtg cttacggccc agcagaggat cgcgatcttc
      181 accatcgtgc agtgggcgct ggcccagccc ttgtttatcg tgacggcgat ctgcgtcgcc
      241 ttctacctcc tcgattgcct gtacgccgag cgcaaggacc gcagcattct cttctggaag
      301 tcgctgccct tgtcggatga gctcacggtc gcctccaagt tcctggtcgc cgccgtggtt
      361 gtgccactcg gagtgttcct actcgccgcc gttgccggcc tcgtgttcgc ggcgatcatc
      421 gccgtgcgtg tcccgggggg gctcacctgg agcacttatg agtggctgcg gaccgagctg
      481 gtgctgctgc tcgaagtcat cctcggtgtg ctctggtact cgcccatcgc cggggcactc
      541 ctcttgttct ccgcctggat ccggcgcaac ccgatcctct gggcctccct cgtacccgtg
      601 gtggccccgg tcgtagagcg cgtcgcgctc ggcacccatt acatctggaa cttcgaggtt
      661 taccggacca acggcatctg gcacaagctg acgctggggc cggatgtctt cggcagcttc
      721 caccacctgc ccccggtggg cgtggtcctc gacaccttca acatccgcgg tgccttcacc
      781 gacatcgacc tgtggtcggg cgtgatcgcc gccatcgcga tggcctacgc cgcagcgcgc
      841 gtccgccgct accgagacga cacctaggcc cggaacgtgg cgctcgggca agcgccggcg
      901 gatcgcttat catcgcaatc cccgacgcct gacaaggccg cgagtgtcag caacggattc
      961 gacagcagag gccgtcgagg tctcccgcac cgctgaaggc ctggagctca ggctgacggg
     1021 cgaatggagg ctgcccgagg tcgccgcgct cgaggccgcg ctcgccgccg tcgacgtggc
     1081 gaccgtcgcg cgcatccgca tcgccgcggc ggggatcacc cggctggatc tctccggcgc
     1141 ctgggcgctc ttcgccttcg tgcggcgcgc gcgccgggcg ggtgccgagg tcggcttccg
     1201 cggcagcccg cccgatgagc tgcgcctggt gcaggagacg ctcgcgagcc aggcattggc
     1261 cgagccggaa gcgtcggcga ccgcggcggc gcccgcggcg ccgggcggcc gcgccctgaa
     1321 cgcgatcggc cgctacgtgg tgcgctcgag ccacgacctc ctcgggggac tcgccttcct
     1381 cggccgcatc ctgatcacct gcgcggcgaa cctggcgcac ccgcggcggc tgcggccgat
     1441 ctcgattgtt cgccacgtct acgagacggg catcaccgcc atgccgatcg tggcgctgat
     1501 cgcctttctc atcagcgtga tcatcgccta catgagcgcc cagcagctgc gcgggctcgg
     1561 cgccgagatc tacgtcgtgg acctcgtcac gatcggcgtg ctgcgcgagc tcggggttct
     1621 gctgacctcg atcattgtcg ccgggcgctc gggcagcgcc ttcgccgccg agctcggctc
     1681 gatgaagctg aacgaggagg tcgacgcgct catcgcgacc ggcgccgacc ccttcgagct
     1741 gctggtggtg ccgcgggtgc tcgggctcgt gatcgccctg ccactgctcg cgatagtggc
     1801 cgatctcatc gggctcgctg gcggagcgct gctgtgccgc tacctgctcg atatgccgct
     1861 cacgcagtac gtgaaccgcg tcagcgaatc catctccccc accactttct gggtggggat
     1921 catcaaggcg cccgtgtttg cgctgctcat cgcgctcgcc ggctgctacc gcggcatgca
     1981 ggtgcggggc tcggcacgcg tgctcggtca catggtgacg gtcgcggtgg tgcaggccat
     2041 attcatggtg atcctcgcgg acgcgctgtt cgccgtgttc ttcatgaagc tgaagatctg
     2101 atggagccgg tagtagaagt ccgcgggctc gtgaaccgct tcgatcgcca ggtcgtgcac
     2161 gacggtctcg acatgcgggt cgatgaggac gaggtcttcg gcatcgtcgg cgggtcgggc
     2221 gccggcaagt cggtgctgct gcgcacgatc ctcggactgc agcgtccggc cgccggcacc
     2281 gtgcgcatcc tcggcagcga catcacccgc cttacggagg agcagctgcg gcccgtcaag
     2341 gcgcgctacg gcgtcgcctt ccaggagggc gcgctgtata cgggccttac ggtgctgcag
     2401 aacgtgcagc tgccgatgat cgagcatctg cggcttccgc aggatgcgct cgagggactg
     2461 gcactgctga aaattcgcct ggtggggctg ccggccgatg ccgcggacaa gtacccgtcg
     2521 cagctctccg ggggcatggt gaagcgcgcg gcggtggccc gcgctctggc gctcgacccg
     2581 gagctgctgt tcctcgatga gcccacctcg ggcctcgacc cggtgagcgc ggcagccttc
     2641 gacgacctgg tggtgtacct gcaacgccat ttgcggctca ccatcgtcat gatcacgcat
     2701 gatctggaca gcatcttccg tacctgcaat cgtgtcgggg tgatcgtcga taagcgcatg
     2761 gaatccgaca cgctcgagcg catcgtcgag cacccgaacc cgtggatcca ggcctacttt
     2821 cacggcgagc gcgcccgcgc ccgcctcgga gccggacatg gagcgtgagg ccaactacgc
     2881 cgccgtcggc gccttcgtgc tgctcgtgac tctcggcggg gcattgttcg tctactggta
     2941 ttccgacacc cgcgagcatc gcgattacaa ccgctacgag atctacttca ccggcagcgt
     3001 cagcggcctg gagcggggcg cggcggtgcg ctatctcggg gtgccggtcg ggcgggtgaa
     3061 cgacctgcgc atcgacccgc gcgactcgag ccgcgccgag gtgatcgtcg acatcggctc
     3121 gagcgccccg gtctccgagc acacggtcgc ggagctgaat ctccagggcg tgacgggcct
     3181 cctgtacatc gacctgagcg aggaccgcag cggcaagcgc ctcgcgccgc cggtcgcgag
     3241 cctcaagtat cccgtgatcc gctcggcgcc ctcgcgcttc gacgtatttc tcgcgggcct
     3301 gcctgagctg gtggccgcag tcggcaatct cgtcaagcgc gccgatcgct tgctgagcga
     3361 tgagaacatc gccgccgtga ccgccaccgt cgccaacctc gacaatgcat cgagcagcct
     3421 gccgcgcacg gtcaatgacc tcaattcgct catcagtgag cttcgcggcg ccagcgccga
     3481 gctcgcggcg agcgcgcatc tcgcgcacgg cattctcgag gccgtggatc cggacgtgcg
     3541 cgccgcggca cagcgcctgc gcgccatcgc cgacagcctc gccgatgcca cccgccagct
     3601 cgacgcggcg atcacggaga accgccagga c
//
LOCUS       DATLKL010000229         1679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_104162, whole genome shotgun sequence.
ACCESSION   DATLKL010000229 DATLKL010000000
VERSION     DATLKL010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104162"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VM713_03000"
     CDS             <1..801
                     /locus_tag="VM713_03000"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83263.1"
                     /translation="TMSQAQRKAADEDDLLVNRMHIPFELDDGIAARAAIGLIVLATD
                     HTIEHEWRQMLGIDGVAFYESRIFNSSTISPETLREMEAGLTDAAAVIRPGERLDVMA
                     YGCTSGAMVMGEEAVFRRIREARPAIPCTTPITAALAAFKALGVRRIALLTPYIDAIN
                     RMMRRYIEERGTAVTAMGSFNHDQDNEVARISPASIEAAALELGAAPNVDMVFVACTS
                     LRVAEHVEAMEARLGKPVSSSNHAMAWHALRLAGYKEPVQGFGQLLRL"
     gene            complement(858..>1679)
                     /locus_tag="VM713_03005"
     CDS             complement(858..>1679)
                     /locus_tag="VM713_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH83264.1"
                     /translation="NMTLLKDHGHAVEFIDVKRCIEIEPALKPIANKLAGAVYCPTDG
                     SGDCRLFTIQLAEACKRLGVEFQLGGQIHGLETRGGRMTAVATERGPVSGDVYVLSLG
                     SYSSIVARSAGFDLPVYPVKGFSLTLPVDPATAPTVGGVDEGTLVAYCGMGDRLRMTA
                     TADFSGYDGSHRPEDFASMLAVARELFPRGVDFSRPRYWACLRPMTPDGPPIIGRAPV
                     ENLWLNTGQGHMGWTMSAGSSRIMVDLLLGRAPALSAAPFTFERYNGTAKAAKAA"
BASE COUNT          287 a          578 c          553 g          261 t
ORIGIN      
        1 accatgagcc aggcacagcg caaggcggcg gacgaggacg atctcctcgt caatcggatg
       61 cacatcccct tcgagctcga tgacggcatt gccgcgcgcg ccgccatcgg cctcatcgta
      121 ctggcgaccg atcacaccat cgagcacgag tggcgccaaa tgctcggcat cgacggcgtc
      181 gccttctacg agagccgcat cttcaactcg agcaccatat cgcccgagac cctgcgcgag
      241 atggaggcgg gcctcaccga cgcggcggcg gtgatccgcc cgggcgagcg cctcgacgtc
      301 atggcctatg gctgcacatc gggtgccatg gtgatgggcg aggaggccgt gttccgccgc
      361 atccgcgagg cgcgtcccgc cattccctgc accaccccga tcaccgcggc gctcgccgcc
      421 ttcaaggcgc tcggggttcg ccgcatcgcc ctcctcaccc cctatatcga cgcgatcaac
      481 cggatgatgc gccgttacat cgaggagcgc gggaccgccg tcacggcgat gggctccttc
      541 aatcacgacc aggacaacga agtggcgcgc atctcgcccg cgtcgatcga ggcggcggcg
      601 ctcgagctcg gtgccgcgcc gaatgtggac atggtcttcg tcgcctgcac gagcctcagg
      661 gtcgccgagc atgtggaggc gatggaggca aggctaggca agccggtgag ctcgagcaat
      721 catgccatgg cttggcacgc gctgcgcctt gccggctaca aggagcccgt ccaaggcttc
      781 ggtcagctgc tgcggctata gccgtaagcc agaaggaatc ggagcagtca ttccgactct
      841 ggaccccggc gctctgatca cgccgccttg gccgcctttg cggtgccgtt gtaccgctcg
      901 aaagtgaacg gcgcggcgga gagcgcaggc gcgcgaccga gcagcaggtc gaccatgatc
      961 cggctcgagc ctgctgacat ggtccagccc atgtgaccct ggccggtatt gagccagagg
     1021 ttctcgaccg gcgcgcggcc gatgatgggc ggcccgtcgg gcgtcatcgg ccggaggcag
     1081 gcccagtacc gcggcctcga gaagtccacc ccgcggggga aaagctcgcg ggcgacggcg
     1141 agcatggatg cgaaatcctc cggccggtgg ctgccgtcat agccggagaa gtcggcggtc
     1201 gcggtcatgc gcagcctgtc gcccataccg caataggcga cgagcgtgcc ctcgtccacc
     1261 ccgcccaccg tgggcgccgt ggccggatcg acgggcaacg tcaaggagaa gcccttgacc
     1321 ggatagactg gcaggtcgaa cccggccgag cgcgccacaa tcgagctgta actgccgagc
     1381 gagaggacgt agacatcccc cgagaccggc ccgcgctcgg tcgcgacggc ggtcatcctg
     1441 ccgcccctgg tctccagccc gtggatctgc ccgccgagct ggaactcgac gccgagccgc
     1501 ttgcacgcct cggccagctg gatcgtgaat agccggcaat cgccgctgcc gtcggtcgga
     1561 cagtaaaccg ctcccgcaag cttgtttgcg atcggcttga gcgccggctc gatctcgatg
     1621 cagcgtttga cgtcgatgaa ttcaaccgcg tgcccgtggt ctttgagcag cgtcatgtt
//
LOCUS       DATLKL010000230         1939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_115529, whole genome shotgun sequence.
ACCESSION   DATLKL010000230 DATLKL010000000
VERSION     DATLKL010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115529"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VM713_03010"
     CDS             <1..672
                     /locus_tag="VM713_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVH83265.1"
                     /translation="VPRDAVDEIIVVDSGSTDRTVERARAAGARVIEERQRGYGRACA
                     AGARAARDCGVIVFLDGDGSDRPELVPLLLAPILAGTHDFVIGSRTRGVREPGSMNKL
                     QIAAGYAAGALTRLLYGVRYTDMCAFRAIRREALDQLRMREMGYGWNLEMQMRAARAR
                     LRILEVPVAHRRRLGGASKVSGTVIGTLRAGIRIGLTFARVACERQPGGQATEVRNPP
                     AQEKP"
     gene            669..1325
                     /locus_tag="VM713_03015"
     CDS             669..1325
                     /locus_tag="VM713_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83266.1"
                     /translation="MTPNPFSDVLQFLTQTAWTTVVYWLLLLGSVAIAVYAWRSIPSQ
                     RSSVQVGNWLCRFFIGSMWWQQTLWKLPPLYTDNPSDPQNSGLHYWMNQLGQSAWLPL
                     QADFVNHVVIPHFYFFAPIVYALEVLTAVSLILGIFVRLWGLIGVLQVLNLWLGLYHA
                     PGEWPWTYFFLLVLMVIFTVNRYGRSLGLDAIIVERTANGRVSGPGAVGSGKLLGMLT
                     "
     gene            1333..>1939
                     /locus_tag="VM713_03020"
     CDS             1333..>1939
                     /locus_tag="VM713_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVH83267.1"
                     /translation="MNHDAAVSSAKQVADRVLSPAARQNDKEGRFSVEAIAALGPAGL
                     LGLMVPEEMGGAALGPRTFAAVVATLAEADASVAMVYLMHVCAATTIAAARAGASVTP
                     TLKDIAAGRHLSTLAFSETGSRSHFWAPLSRARRNGAGVYLTAKKSFVTSAGHAQSYV
                     VSSLAPEGKGPTDSTLYLVAAGARGLSVAGPWDGLGMRANAS"
BASE COUNT          297 a          642 c          635 g          365 t
ORIGIN      
        1 gtcccgcgcg atgcggtcga tgagatcatc gtcgtcgaca gcggcagcac ggatcgcacc
       61 gtcgaacgcg cccgcgccgc gggtgcacgt gtcatcgaag aacggcagcg cggctacggc
      121 cgcgcctgtg ccgcgggcgc ccgggcggcg cgtgactgcg gcgtgatcgt gttcctcgac
      181 ggtgacggca gcgatcggcc ggagctcgtg ccgctcctgc tcgctccgat tctcgccggc
      241 acgcacgact tcgtgatcgg cagccgcacc cgcggcgtgc gcgagcccgg cagcatgaac
      301 aagctgcaga tcgcggccgg atacgctgcc ggcgcgctga cgcgcctgct gtacggtgtc
      361 aggtataccg acatgtgcgc cttccgcgcc atccgtcgcg aggctctcga tcagctccgc
      421 atgcgcgaga tgggttacgg ctggaacctt gaaatgcaaa tgcgcgccgc ccgggcccgg
      481 ctgcgcatcc tcgaggtgcc ggtcgcgcat cgccgccggc tgggtggcgc ttccaaggta
      541 tcgggcaccg ttatcggaac acttagagcc ggaattcgca tcggcctcac gtttgcgcgc
      601 gtggcctgcg aacggcaacc agggggtcag gcgactgagg tccgcaaccc acctgcgcag
      661 gagaaaccat gacacccaat ccattttccg atgtgctgca gttcctgacg cagaccgcct
      721 ggacgaccgt ggtctactgg ctgctgctcc tcggcagcgt cgccatcgcg gtctatgcgt
      781 ggaggagcat cccgagccaa cgctcgtcgg tccaggtcgg caactggctg tgccgcttct
      841 tcatcggctc gatgtggtgg cagcagacgc tctggaagct gcctccttta tataccgaca
      901 atccgagtga cccgcagaac agcggccttc actactggat gaatcagctc ggccagtcgg
      961 cctggctgcc gctgcaggcg gacttcgtca atcatgtcgt aattccgcat ttctatttct
     1021 tcgcgcccat cgtctatgcc ctcgaggtgc tgaccgcggt gtcgctcatc ctcggcatct
     1081 tcgtacgtct ctggggcctc atcggtgtgc tgcaggtcct caatctctgg cttggcctgt
     1141 atcacgcccc cggcgagtgg ccatggacct acttcttcct gttggtgctg atggtgatct
     1201 tcaccgtgaa tcgatatggc aggagcctgg gactcgatgc gatcatagtt gagcgtacgg
     1261 cgaacgggcg cgtgagcggc cctggcgccg tcggctccgg caagttgctc ggcatgctga
     1321 catgagggtg tcatgaatca cgatgctgcg gtctctagtg ctaagcaggt ggccgatcgc
     1381 gtgctctcgc ccgcggcccg gcagaatgac aaggaaggcc gcttctcggt ggaagcgatc
     1441 gcggcccttg gcccggcggg actgctcggg ctgatggtgc ccgaggagat gggtggagcc
     1501 gcgctggggc cgcgtacctt cgccgcggtc gtcgcgaccc tggccgaggc tgacgcctcg
     1561 gtagccatgg tctatttgat gcacgtctgc gctgccacga ccatagccgc ggcgcgtgcc
     1621 ggcgcctcag tcacgccgac cttaaaggac atcgcagccg gccggcatct gtccacgctc
     1681 gccttcagcg aaaccggctc gcgcagccac ttctgggcgc cgctctcgcg tgcgcgccgc
     1741 aacggggcgg gtgtgtatct cactgcaaag aaatccttcg tcacgagtgc cggccacgcg
     1801 caaagctacg tggtctcatc gcttgcgccc gaaggcaaag gaccgaccga ctcgacgctg
     1861 tatctggtcg cggccggtgc gcgcggactg tcggtcgcgg gcccctggga cggactcggc
     1921 atgcgagcca atgcttccg
//
LOCUS       DATLKL010000231         1795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_81583, whole genome shotgun sequence.
ACCESSION   DATLKL010000231 DATLKL010000000
VERSION     DATLKL010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81583"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VM713_03025"
     CDS             complement(<1..1152)
                     /locus_tag="VM713_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HVH83268.1"
                     /translation="MNDLTKNILLWVVIVIVLLLVFSRYMPTAGQPQQIAYSTFLDDV
                     KNNRVESVVLQGDMIYGEHKDKTAFKTFNPETDYTALIGTLEKADVRIEGQPPKPPNY
                     MGQLLLQLAPALLLIVVFVYMLRQMQGASGGRGAMSFGKSRARLLGEDQVSVTFADVA
                     GVDEAKQEVGEIVDFLKDPGKFQKLGGKIPKGVLMVGSPGTGKTLLARAIAGEAKVPF
                     FTISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNQLLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREQ
                     ILRVHMRRVPLADDVKPALIARGTPGFSGADLANLVNEAALFAARANKRI"
     gene            complement(1195..1788)
                     /locus_tag="VM713_03030"
     CDS             complement(1195..1788)
                     /locus_tag="VM713_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HVH83269.1"
                     /translation="MKEHFRDTYVQRAQSGGWRSRAVFKLEEIDRREKLLKPGAVCLD
                     LGAAPGGWSQYARGRVGPTGRVIATDILPMEAIPGVEFLQGDFREEDALGRLLALLPE
                     GRADVVLSDMAPSLSGVDVIDQPRSMHLAELALEMARRVLKPGGVALIKVFQGSGFED
                     LLKAARPEFGRVKLVKPLASRARSPEMYLLAMKYRLV"
BASE COUNT          290 a          605 c          600 g          300 t
ORIGIN      
        1 gatgcgcttg ttggcgcgcg cggcgaacag cgccgcctcg ttgacgaggt tggcgaggtc
       61 tgcgcccgag aagccaggcg tgccgcgcgc gatcagcgcc ggcttgacgt cgtcggcgag
      121 cggcacacgc cgcatgtgca cgcgcaggat ctgctcgcgg ccgcgcacgt ccggcagcgg
      181 cacgaccacc tggcggtcga agcggcccgg gcgcagcagc gccgggtcga gcacgtccgg
      241 acggttggtg gcggcgatga cgatgatgcc ctcgttgccc tcgaagccgt ccatctccac
      301 cagcagctga ttgagcgtct gctcgcgctc gtcgtgtccg ccgccgaggc ccgcgccgcg
      361 gtgccggccg acggcgtcga tctcgtcgat gaagatgatg cagggagcgt gcttcttcgc
      421 ctgttcgaac atgtcgcgta cgcgcgaggc gccgacgccg acgaacatct cgacgaagtc
      481 ggagccggag atggtgaaga acggcacctt ggcttcgccg gcgatcgcgc gcgccagcag
      541 cgtcttgccg gtgccgggcg atcccaccat cagcacgccc ttggggatct tgccgccgag
      601 cttctggaac ttgccggggt ccttgaggaa gtcgacgatc tcgccgacct cctgcttagc
      661 ctcgtcgacg ccggcgacgt cggcaaacgt gacgctcacc tgatcctcac cgaggagccg
      721 cgcgcggctc ttgccgaagg acatggctcc ccggccgccg gatgcgccct gcatctggcg
      781 cagcatgtag acgaacacca cgatcaggag cagtgccggc gcgagctgca gcagcagctg
      841 ccccatgtag ttcggcggct tgggaggctg gccctcgatg cgcacgtcgg ctttctcgag
      901 cgtgccgatg agagcggtgt agtcggtctc ggggttgaag gtcttgaagg cggtcttgtc
      961 cttgtgctcc ccgtagatca tgtcgccctg cagtaccacc gactcgacgc ggttgttctt
     1021 cacgtcgtcg aggaaggtgg aataggcgat ctgctggggc tgtcccgcgg tcggcatgta
     1081 gcggctgaac accagcaaca gcacgatcac gatcacgacc cagagcagga tgttctttgt
     1141 caagtcgttc aaaggcttcc gtcctttaga agggggtgcc tcggctcccc gaagctacac
     1201 caagcgatac ttcatggcca gcaagtacat ctcagggctc cgggcccggg atgccagtgg
     1261 tttgaccagc ttcacccgcc cgaattcggg gcgcgcggcc tttagcagat cctcaaagcc
     1321 cgaaccctgg aaaaccttga tcagggcgac cccgcccggc ttcaagacac gcctggccat
     1381 ctcgagggcg agctcggcga gatgcatcga gcgcggctgg tcgatcacat cgaccccact
     1441 cagtgacggg gccatgtccg agagcaccac gtcggccctt ccctcgggca ggagggcgag
     1501 caacctgccg agcgcgtcct cctcgcggaa gtccccctgg aggaactcga ccccggggat
     1561 tgcctccatg ggcaggatgt cggtggcgat gacgcgcccc gtggggccca cccgaccccg
     1621 ggcgtactgg ctccagccgc ccggagcagc tcccaggtcg aggcagacgg cgccgggctt
     1681 caggagtttc tcgcggcggt cgatctcctc gagcttgaac acggcccgcg agcgccagcc
     1741 gccgctctgg gcgcgctgga cgtaagtgtc ccggaagtgc tccttcagcc accga
//
LOCUS       DATLKL010000232         2350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_2706, whole genome shotgun sequence.
ACCESSION   DATLKL010000232 DATLKL010000000
VERSION     DATLKL010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2706"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VM713_03035"
     CDS             <1..407
                     /locus_tag="VM713_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVH83270.1"
                     /translation="FSVVVGASGSGKTTLLNLIGCIDTPSSGTLEVCGEHVAALSDDA
                     LSDFRARNIGFIFQTFSLVPVLSAYENVEYPLLLVGMPEAERRRRTLAMLDAVGLAAQ
                     ARQRPNELSGGQRQRVAIARALVKEPQVVLAD"
     gene            456..593
                     /locus_tag="VM713_03040"
     CDS             456..593
                     /locus_tag="VM713_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83271.1"
                     /translation="MRRVQTQSRTTFIFATHDPQLMSHADETFVIRDGALVEHRTGAA
                     L"
     gene            590..1999
                     /locus_tag="VM713_03045"
     CDS             590..1999
                     /locus_tag="VM713_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVH83272.1"
                     /translation="MRLLLKIAFRNMLRNLRRSLMTGSAVAAGALALLLFGGFAAYIF
                     AGLETNNVQRIGHLTVFRSGYFVFGAGNPAAYGIDRYQDVMQLIERDPVVGPMINVIT
                     PTQSLVGIAGNFSGGVEASKTFLGTGLIPSNRERMRQWDEFRASAGYVPDRRMSDADA
                     ARGVVGVGLARVLGLCAPLGLSDCPPLPAVEPVPGESAPPADPELAALAARDLAADPA
                     RSGQAPRLDLLAATAGGAPNVVQLAVGGAEPQGVKELDDNFVAMPLGLAQQLVYGRGE
                     HKATGIVLQLKRSEDLRAARARLSALFGQHHLDLEVRDSGELSPFYGQVVKMFSSIFL
                     FIALVMGVIVLFAVINTMTMNVMERTNEVGTIRALGVRRAGIRAQFTVEGVLIGAIGA
                     TVGAALALAIASLINHAGLTWVPPGNASAVPLRLDVSGRPLLVAGAWLGLSIVTTLAA
                     LLPARRAARLPVVDALRHV"
     gene            2008..>2350
                     /locus_tag="VM713_03050"
     CDS             2008..>2350
                     /locus_tag="VM713_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83273.1"
                     /translation="MKYRFAAVPLALALFLAATAHAQTAEEIVAATDKVRNPAQPFRS
                     TLRLTEYVGGQERSHDSLVLFSKEDRATGQFRNLVQYVEPARDAGKRVLLDGHSLWFY
                     DPNSKASVRISA"
BASE COUNT          330 a          854 c          785 g          381 t
ORIGIN      
        1 gcttttcggt ggtggtcggc gcctcgggca gcggcaagac gacgctgctg aatctcatcg
       61 gctgcatcga caccccgagc agcggcaccc tggaggtgtg cggcgagcac gtcgccgcgc
      121 tctcggacga tgcgctgtcc gacttccgcg cccgcaacat cggcttcatc ttccagacct
      181 tcagcctggt gccggtgctg tcggcctacg agaacgtcga gtacccgctg ctgctggtcg
      241 gtatgccgga ggccgagcgc cggcgccgca ccctcgcgat gctggacgcg gtcgggctcg
      301 ccgcccaggc ccgccagcgg cccaacgagc tctccggcgg ccagcgccag cgcgtggcga
      361 tcgcccgcgc cctggtcaag gagccgcagg tcgtgctcgc cgactagccg acggcgaatc
      421 tcgactcggc gaccggcgcc gcgatcatcg agctgatgcg gcgcgtccag acgcaatcgc
      481 gcaccacctt catcttcgcc acccacgacc cgcagctgat gtcgcacgcc gacgagacct
      541 tcgtcatccg cgacggcgcg ctggtcgagc accgcacagg agccgcgcta tgagactgct
      601 gctgaaaatt gccttccgca acatgctgcg gaatctccgc cgctcgctca tgaccgggtc
      661 ggcggtggcc gcgggagcgc tcgcgctgct cctgttcggt ggattcgccg cctacatctt
      721 tgcgggcctc gagaccaaca atgtgcagcg catcgggcat ctgaccgtat tccgctcggg
      781 ctacttcgtg ttcggcgccg gcaacccggc cgcctacggc atcgatcgct accaggacgt
      841 gatgcagctc atcgagcgcg acccggtagt cggaccgatg atcaacgtca tcacgccgac
      901 ccagtcgctg gttggcatcg ccggcaattt cagcggcggc gtcgaggcct cgaagacctt
      961 cctcggcacc ggcctcattc cctcgaaccg cgagcgcatg cggcagtggg atgagtttcg
     1021 cgccagcgcc ggctacgtgc ccgaccgccg catgtcggat gccgacgctg cgcgtggggt
     1081 ggtcggggtc gggcttgccc gtgtgctggg gctgtgcgcg ccgctcggcc tgagcgactg
     1141 cccgccgctg ccggcggtgg agccggtgcc gggggagagc gcgccgccgg ccgaccccga
     1201 gctcgccgca ctcgcagccc gcgatctcgc cgcggatccg gcgcgctccg gacaggcgcc
     1261 gcgtctcgat ctgctggctg cgaccgcggg cggtgcgccg aacgtggtgc agctcgccgt
     1321 cggcggcgcc gagccgcagg gcgtcaagga gctcgacgac aacttcgtcg ccatgccgct
     1381 cgggctcgcc cagcagctgg tgtacgggcg cggcgagcac aaggccaccg gcatcgtgct
     1441 gcagctcaag cgcagcgagg acctgcgcgc ggcgcgcgcc cgcctgagcg cgctcttcgg
     1501 gcagcatcac ctcgatctcg aggtgcgcga ctctggcgag ctgtcaccct tctacggaca
     1561 ggttgtgaag atgttcagct cgatcttcct gttcatcgcg ctggtcatgg gtgtgatcgt
     1621 gctgttcgcc gtcatcaaca ccatgaccat gaacgtgatg gagcgcacca acgaggtcgg
     1681 cacgatccgc gcactcggcg tgcggcgcgc tggaatccgc gcgcagttca cggtcgaagg
     1741 cgtcctgatc ggcgcgatcg gtgccacggt cggcgccgcc ctggccctag ccatcgcctc
     1801 cctcatcaat cacgcagggc tcacctgggt gccgcccggc aacgccagcg cggtgccgct
     1861 gcggctcgat gtctccggcc gcccgctgct ggtcgccggt gcctggctcg gcctttcgat
     1921 cgtcaccacg ctcgcggcgc tcctgcccgc caggcgcgca gcgcggctgc cggtggtcga
     1981 tgcgctccgc cacgtatgag gtttgccatg aagtaccgat tcgcagctgt gccgctcgcc
     2041 ctggcgctgt tcctcgccgc caccgcccac gcccagacgg ccgaggagat cgtggccgcg
     2101 accgataaag tccgaaaccc ggcgcagccg ttccgcagca cgctcaggct caccgagtac
     2161 gtcggcgggc aggagcgcag tcacgactcg ctggtcctgt tctcgaagga ggatcgcgct
     2221 accgggcagt tccgcaacct cgtgcagtac gtcgagccgg cgcgcgatgc cggcaagcgc
     2281 gtgctgctcg atgggcactc gctgtggttc tacgacccga actccaaggc cagcgtacgc
     2341 atctcggcgc
//
LOCUS       DATLKL010000233         1617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_183364, whole genome shotgun sequence.
ACCESSION   DATLKL010000233 DATLKL010000000
VERSION     DATLKL010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183364"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VM713_03055"
     CDS             complement(<1..833)
                     /locus_tag="VM713_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HVH83274.1"
                     /translation="MHPYRTHNCGELRLEQAGIEARLSGWVHRKRDHGQLLFIDLRDH
                     YGLTQCVVDVSSPLFPAIEQLRLESVVTMTGTVVARSRETVNPALPTGTVELQIADFK
                     LLSAADPLPLQVNADRDENEENRLRYRFLGLRRERMHRNIMLRARVIQSIRRRTIEQG
                     FTEFQTPILTASSPEGARDYLVPARLHPGKFYALPQAPQQFKQLLMVAGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDFEMSYVTQEDVFAAIEPVLHGVFAEFGGDRRVSPAPFP
                     R"
     gene            1010..>1617
                     /locus_tag="VM713_03060"
     CDS             1010..>1617
                     /locus_tag="VM713_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83275.1"
                     /translation="MSLIADTDGLARFCRGLASSSYLAVDTEFMRDRTYFPQLCLIQV
                     AGDEEAAAIDALSTGLDLAPLLALLDRPDLLKVFHAGRQDIEIFYLLSGKVPAPMFDT
                     QVAAMVCGFGDSVAYDTLVSKLTGTHIDKSSRFTDWAVRPLTERQIRYAIEDVTHLRR
                     VYERLKRRLERSGRAAWLAEEMAVLSDPATYRNDPANAWLRL"
BASE COUNT          271 a          527 c          538 g          281 t
ORIGIN      
        1 atgcggggga atggcgccgg gctgacccgg cggtcgccgc cgaattctgc gaagacgccg
       61 tgcaacaccg gctcgatcgc ggcgaagacg tcctcttgcg tcacatacga catttcgaaa
      121 tcgagctggt agaactctcc gggagagcgg tcggcccgcg cgtcctcgtc gcggaagcag
      181 ggtgcaatct gaaaatatcg gtcgaagccc gccaccatga ggagctgttt gaactgctgg
      241 ggcgcctggg ggagcgcgta gaacttcccg ggatggagcc gcgccggcac gagataatcg
      301 cgcgcaccct cgggcgaact cgcggtcagg atcggcgtct ggaactcggt gaagccctgc
      361 tcgatcgtgc gccggcggat gctctggatc acgcgcgcac gcagcatgat gttccggtgc
      421 atgcgttcgc gccgcaggcc gaggaagcgg tagcgaaggc ggttctcctc gttctcatcg
      481 cggtcggcat tgacctgtag cggcagcggg tccgcggcag agagcagctt gaagtcggcg
      541 atctgcagct ccaccgttcc ggtcggaagc gccggattga ccgtctccct gctgcgcgcg
      601 accactgtgc cagtcatcgt cacaacgctc tcaagacgca gctgctcgat ggcgggaaac
      661 agggggctcg agacgtcgac cacgcactgg gtcaggccgt aatggtcgcg caggtcgatg
      721 aagagcagct gaccatggtc ccgcttgcga tgcacccagc ccgagagccg ggcctcgatg
      781 cccgcctgct cgagcctgag ctcgccgcaa ttgtgggtcc ggtagggatg catggggagg
      841 cctcgcgcgg gtaccttgcg gatggtcgaa gttgaggcaa ggtgcggctt tcgtgccggg
      901 ccgcaacagc gcatggggcg gccgatttgt caagccgtgg aagcccggcg gaaccgcgcc
      961 gccgggcgcg cttttgccat gccgagaaag cgtgtaagag agggcggcga tgagcctgat
     1021 cgccgacacc gatggcctcg cccggttctg ccggggcctc gcttcctcct cctacctcgc
     1081 ggtcgacacc gaattcatgc gggaccggac ctactttccg cagctgtgcc tgatccaggt
     1141 cgcgggcgac gaggaggcag ccgcgatcga tgcgctatcg accggcctcg accttgcgcc
     1201 gctgctcgcg ctgctcgatc ggcccgacct tctcaaagtc tttcatgccg gccgccagga
     1261 catcgaaatc ttctatctgc tctccggcaa agtgccggcc cccatgttcg acacgcaggt
     1321 cgccgccatg gtgtgcggct tcggcgattc ggtcgcatat gacacccttg tctctaagct
     1381 caccggcacg catatcgaca agtcgtcgcg ctttaccgac tgggcggtgc ggcccttgac
     1441 cgagcgccag atccgctacg ccatcgagga cgtcacccat ctgcgcaggg tctacgagcg
     1501 actcaagcgc cgactggagc gctcgggacg cgccgcttgg ctcgcggagg agatggcggt
     1561 gctctccgat cccgccacct atcgcaacga tcccgcgaac gcttggctcc ggctgaa
//
LOCUS       DATLKL010000234         1868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_13807, whole genome shotgun sequence.
ACCESSION   DATLKL010000234 DATLKL010000000
VERSION     DATLKL010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13807"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /gene="mazG"
                     /locus_tag="VM713_03065"
     CDS             complement(<1..705)
                     /gene="mazG"
                     /locus_tag="VM713_03065"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HVH83276.1"
                     /translation="MSEAPRAIEALIELMARLRDPQRGCPWDLRQSFDTIAPYTIEEA
                     YEVADAIERGEHERLRDELGDLLFQVVFHARMAEERGWFDFADVAESIHAKLVRRHPH
                     VFAGAAIEERHLVREWEAQKAREREQTAGAADERGTLAGVPQALPALTRAAKLGRRAA
                     RVGFDWPDAAGARAKVDEELGELDAALEGSGASAAGVGSEAAGELGDLLFAVVNLSRH
                     LALDAETVLRGANAKFE"
     gene            complement(713..1405)
                     /locus_tag="VM713_03070"
     CDS             complement(713..1405)
                     /locus_tag="VM713_03070"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HVH83277.1"
                     /translation="MAERPESATGERDWAAIIRAKLAGSVPRHAAEDWILPGLSAADS
                     QIYRRSLPTAPTPAAVLIPLVEHRRPTVLLTERAVGLRTHAGQVSFPGGHIETSDPSP
                     AAAALREAREEIGLAEALVSVAGYLPDHIVITGFRITPVVGFVRSGFALALDRKEVQG
                     TFEVPLDYIFDPSNFRKERRRLIRSGEEVEFWDIPYEGRTIWGATAGILLTLYRLCVG
                     PLPFERAGRTAQ"
     gene            complement(1398..>1868)
                     /locus_tag="VM713_03075"
     CDS             complement(1398..>1868)
                     /locus_tag="VM713_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HVH83278.1"
                     /translation="GSSIMPGKVNPVIPEATTMVAAQVIGNDTAITIAGQSGNFQLNV
                     MLPVIAYNLLGSIALLANVSRLLADRAIEGFKVNAARIAEALDRNPILVTALNPVIGY
                     EKGAAIAKAAYAQGRPILEVAREMTGLPAAELEKLLDPAALTRGGIKGGTSGGG"
BASE COUNT          277 a          687 c          613 g          291 t
ORIGIN      
        1 ctcgaacttc gcgttggcgc cgcgcagcac ggtctcggcg tcgagcgcca gatgccggct
       61 gagattcacg actgcaaaga ggaggtcgcc cagctcgccg gcggcttcgc tcccgacacc
      121 cgccgccgac gcgccgcttc cctcgagcgc ggcatccaac tccccgagct cctcatcgac
      181 cttggcgcga gcccccgcgg catccggcca gtcgaagccg acgcgcgccg cgcggcgtcc
      241 aagcttcgca gcgcgcgtga gtgcaggaag cgcctgcggc acgccggcca gcgtgccgcg
      301 ctcgtcggcc gctccggcgg tctgctcgcg ctcgcgcgcc ttctgcgcct cccactcgcg
      361 cacgaggtgc cgctcctcga tggcggcgcc ggcgaacaca tgcgggtggc ggcggacgag
      421 cttcgcatga atggactcgg cgacgtccgc aaaatcgaac cagccacgct cctcggccat
      481 gcgggcgtga aacacgacct gaaagagcag gtctccgagc tcgtccctca gccgctcgtg
      541 ctcgccgcgc tcgatcgcgt cggcgacctc gtacgcctcc tcgatggtat agggagcgat
      601 ggtgtcgaag ctctgcctga gatcccaggg gcagccacgc tgcggatcgc gcagccgtgc
      661 catgagctcg atgagtgcct cgatcgcgcg cggtgcctcg ctcatcacca cctcactgcg
      721 ccgtgcgccc agcgcgctcg aacggcaacg gtccgacgca cagccgatag agcgtcagga
      781 ggatgcccgc ggtcgcgccc cagatggtgc gcccctcgta tgggatgtcc cagaactcga
      841 cctcctcgcc actgcggatg aggcggcggc gctctttgcg gaagttgctc ggatcgaaga
      901 tgtagtcgag cggtacctcg aaggtcccct gcacttcctt gcggtcaagc gccagcgcga
      961 atcccgagcg cacgaacccc accaccggcg tgatgcgaaa gcccgtgatg acgatgtggt
     1021 ccggaaggta gcccgccacc gataccagtg cctcagcgag accgatctcc tcgcgcgcct
     1081 cacgcagcgc cgccgccgcg gggctcgggt cgctcgtctc gatgtgcccc cccgggaagc
     1141 tcacctggcc cgcatgcgtg cgcagcccca cggcgcgctc cgtcaacagc acggtcggac
     1201 gccgatgctc gacgagtgga atcaacaccg cagcgggcgt cggcgccgtc ggcagtgagc
     1261 gccgatagat ctggctgtcc gccgcggaca gtcccggcag gatccagtcc tcggcagcgt
     1321 gacggggaac gctgccggcg agcttcgcac gaatgatcgc cgcccagtcg cgctcgcccg
     1381 tcgcgctttc cggcctctca gccaccgccg ctcgtgccgc ccttgatccc gccgcgggtg
     1441 agcgccgcgg ggtcgagaag cttctcgagc tcggccgccg gaagcccggt catctcgcgg
     1501 gcgacctcga ggatcggccg gccctgcgcg tatgccgcct tggcgatcgc cgcgcccttc
     1561 tcgtaaccga ttaccggatt caaggcggtc accaggatcg ggttgcgatc gagcgcctcg
     1621 gcgatgcgcg cggcgttcac cttgaatccc tcgatcgcgc ggtccgcgag cagccgcgat
     1681 acgttggcga gcagcgcgat gctcccgagc aggttgtagg caatgaccgg cagcatgacg
     1741 ttcagctgga agttgcccga ctgcccggcg atcgtgattg ccgtgtcgtt gccgatcacc
     1801 tgcgccgcta ccatggtggt ggcctcgggg atcaccgggt tcaccttgcc gggcatgatg
     1861 ctgctgcc
//
LOCUS       DATLKL010000235         1590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_13808, whole genome shotgun sequence.
ACCESSION   DATLKL010000235 DATLKL010000000
VERSION     DATLKL010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13808"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VM713_03080"
     CDS             <1..688
                     /locus_tag="VM713_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVH83279.1"
                     /translation="DPVREILVELIDFDGLKGRGAIPLFIAATNLRDGGRRIFTNAEL
                     SPDVLIASACLPTLHHAVKLDDGHYWDGGFSANPPLLPLLEQCDGAEILLVHVNPMTE
                     PDVPMTAREIHSRLNNIVFNGPVKRELEMIAWLRSAIERAGGRDSPLGKRLAQLKIHS
                     IGADELMRRLGTASKLNPDWRFVTYLKQAGEQAAERWLEDVFGAAVEPLDTTAPARGG
                     AVAPFRAPSR"
     gene            700..1494
                     /locus_tag="VM713_03085"
     CDS             700..1494
                     /locus_tag="VM713_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HVH83280.1"
                     /translation="MPVALHRMYRYPVKGMTGESLEHVALRAGERLPGDRRFAIAHGG
                     TTGPETRFEWRPRSDFLMLARNERLALLEARYNETSCTLTLLRHGRSVARGNPGETLG
                     RAVIEQFFAAFMGTELRGAPKLVEAAEGGFTDSREGWLSLINLASVRDLERVVRAPID
                     PLRFRANLYIDGLAPWRERDWVGGEIAVGAARLRVMECITRCAATNVNPATGARDLNI
                     PLALQQGIGRGEMGVYAKVITAGTIAAGDAVALPFDEARGEASLSA"
BASE COUNT          268 a          540 c          518 g          264 t
ORIGIN      
        1 cgacccggtg cgcgagatcc ttgtcgagct catcgatttc gacgggctta agggaagagg
       61 ggcgatcccc ctcttcatcg cggcgaccaa cctgcgggac ggcgggcggc gtatcttcac
      121 caatgcggag ctgtcgcccg acgtactgat cgcgtcggcc tgcctgccga cgcttcatca
      181 cgccgtcaag ctcgatgacg gccactactg ggacggcggc ttcagcgcaa accccccact
      241 cctcccgctg ctcgagcaat gcgacggcgc ggaaatcctg ctcgttcatg tcaatccgat
      301 gaccgagccc gacgtgccaa tgaccgctcg cgaaatccac tcgcgtttga acaacattgt
      361 gttcaacggc ccagtgaagc gcgagctcga gatgattgcc tggctccgca gcgcgatcga
      421 gagggccggc ggcagggaca gtccgctcgg gaagcggctt gcgcagctga agatccattc
      481 cattggcgcc gacgagctca tgcgcagact gggcacggcg agcaagctca atccggattg
      541 gcgcttcgtc acctatctga agcaagcggg cgaacaagca gccgagcgct ggctcgagga
      601 cgttttcggc gccgccgttg agccactcga caccaccgcg cccgcccgcg gcggcgcggt
      661 tgcgcccttc cgcgctccaa gtcgataggg tcggcgacca tgcccgtggc cctccaccgc
      721 atgtatcgct atcccgtcaa aggcatgacc ggcgagtcgc tcgagcacgt ggccctccgc
      781 gcaggcgagc gacttccggg cgaccgccga ttcgccatcg cgcacggcgg cactaccggc
      841 ccggagacaa ggttcgagtg gcgaccgagg agcgactttc tcatgctcgc gcgcaacgag
      901 cgcctggccc ttctcgaggc gcgctacaac gagaccagct gcaccttgac gctgctgcgc
      961 cacggcagat cggtggcgcg cggcaatccg ggcgagacgc ttggtcgtgc cgtgatcgag
     1021 caattcttcg ccgccttcat ggggaccgag ctgcgcggcg ctcccaagct ggtcgaagcc
     1081 gcagagggcg ggttcaccga ttcccgagag ggctggctat cgctcatcaa tttggcgagt
     1141 gtgcgcgatc tcgagcgcgt cgtccgcgca cccatcgacc ctctgcgctt tcgcgccaat
     1201 ctctacatcg acggtcttgc gccctggcga gaacgcgatt gggtcggcgg cgagattgcg
     1261 gtgggagcgg cccgcctgag ggtcatggag tgcatcaccc gctgcgcggc gacaaacgta
     1321 aatcccgcga cgggcgcgcg cgacctcaac atccccttgg ccctccaaca gggtatcggc
     1381 cgcggcgaga tgggcgtcta tgccaaggtg atcacggcgg gcaccattgc cgcaggcgac
     1441 gcggtggccc tgccgttcga cgaagcgcgc ggcgaggcaa gtctatcggc ctgactcgat
     1501 cgtcggtacc ggctttgcgc ggatcagtaa ggctgcgggt ccgtgccgtc gttcgacccg
     1561 ttgatcgggc ccgactgtcc cggaccggga
//
LOCUS       DATLKL010000236         1601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_205591, whole genome shotgun sequence.
ACCESSION   DATLKL010000236 DATLKL010000000
VERSION     DATLKL010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205591"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1601)
                     /locus_tag="VM713_03090"
     CDS             complement(<1..>1601)
                     /locus_tag="VM713_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HVH83281.1"
                     /translation="AYPQEALEERAGDATRRELRAAYNRALEQIGAEGVAELRAWPER
                     VRSATDPTYSYKVRERTVKGENYTESLSRSTIPKLAVPGLRDWGELLHFLLLENLPGA
                     YPYTGGVYPYRREEEDPTRMFAGEGGPERTNRRFHYLAHGHGAARLSTAFDSTTLYGE
                     DPDTRPDVYGRTGNSGVSIATLDDMKKLYSGFDLCSPSTSVSMTINGPAPMLLAMFMN
                     TAVDQQVERYLRAEGRWSAAEQKIAALNAAAAARGVAPPTYKGALPAHHDGSGLALLG
                     VTGDELLEREVYERIRAETLEAVRGTVQADILKEDQAQNTCIFSTEFALRMMGDVQQY
                     FSERRVRNFYSVSISGYHIAEAGANPISQLAFTLANGFTIAEYYLARGMKIDDFAPNL
                     SFFFSNGMDPEYAVIGRVARRIWARAMRERYHAGPRSQMLKYHVQTSGRSLHAREISF
                     NDIRTTLQALYALFDNCNSLHTNAYDEALTTPTEESVRRAVAIQLIINRELGLNRIQN
                     PWQGSFAIDYLTDLVEEAVYKEFEA"
BASE COUNT          243 a          510 c          563 g          285 t
ORIGIN      
        1 cgcctcgaac tccttgtaga ccgcttcctc gaccaggtcg gtgagatagt cgatggcgaa
       61 cgagccctgc cacggattct ggatccgatt gagcccgagc tcgcggttga tgattagctg
      121 gatggcgacc gcgcgccgca cgctctcctc ggtcggcgtg gtgagcgcct catcgtaggc
      181 gttggtgtgc aggctgttgc agttgtcgaa cagcgcgtac agcgcctgca gcgtcgtgcg
      241 gatgtcgttg aaggagatct cgcgtgcgtg gagactccgg ccggaggtct gcacgtggta
      301 cttgagcatc tggctgcgcg gccccgcgtg gtagcgctcg cgcatggcgc gcgcccagat
      361 gcggcgcgcc acgcgaccga tgacggcgta ctccggatcc atgccgttgg agaagaagaa
      421 cgacaggttg ggcgcgaagt cgtcgatctt catgccgcgc gccaggtaat actccgcaat
      481 ggtgaagccg ttggcgagcg tgaaggcgag ctgggagatc gggttggcgc cggcctcggc
      541 gatgtgatag ccggagatcg acacggagta gaagttgcgc acgcgccgct cgctgaagta
      601 ctgctgcacg tctcccatca tgcgcagcgc gaattcggtc gagaagatgc aggtgttctg
      661 cgcctggtcc tccttgagga tgtcggcctg tacggtgccg cgcaccgctt ccagcgtctc
      721 ggcgcggatg cgctcgtaga cctcgcgctc gaggagctca tcgcccgtca ctccgaggag
      781 cgccagcccc gagccgtcgt ggtgcgccgg cagcgcgccc ttgtaggtcg ggggcgccac
      841 gccgcgcgcc gccgccgcgg cgttcagggc ggcgatcttc tgctcggccg cactccagcg
      901 cccctcggcc cttaggtaac gctccacctg ctggtcgacg gcggtgttca tgaacatcgc
      961 gagcagcatc ggcgccggcc cgttgatggt catcgagacc gaggtcgagg gcgagcacag
     1021 gtcgaagccc gagtagagct tcttcatgtc atcgagagtc gcgatcgata cgccggagtt
     1081 gccggtgcgg ccgtagacat cgggccgggt gtccgggtcc tccccataga gggtggtcga
     1141 atcgaaggcg gtcgagaggc gcgccgcgcc gtgtccgtgc gccaggtaat ggaagcgccg
     1201 gttggtgcgc tccggccctc cttcgccggc gaacatgcgg gtcggatcct cctcctcgcg
     1261 ccgataggga tagacgccgc ccgtgtaggg ataggcgccg gggagattct ccagaagcag
     1321 gaaatgcagc agctcgcccc aatcgcgcaa gccgggcacg gcgagcttgg ggatcgtcga
     1381 gcgcgacagg ctctcggtgt agttctcgcc cttcaccgtg cgttcgcgca ccttatagga
     1441 gtaggtgggg tcggtggccg agcgcacccg ctcgggccag gctcgaagct ccgctacacc
     1501 ctcggcgccg atctgctcga gcgcgcggtt gtaggccgca cgcagctcac gccgcgtcgc
     1561 atcgcctgcg cgttcctcga gcgcttcctg cgggtaagcc t
//
LOCUS       DATLKL010000237         2111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_183372, whole genome shotgun sequence.
ACCESSION   DATLKL010000237 DATLKL010000000
VERSION     DATLKL010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183372"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..>2111
                     /locus_tag="VM713_03095"
     CDS             255..>2111
                     /locus_tag="VM713_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVH83282.1"
                     /translation="MTMTADNNSNSAKTVGGGLFNRYILKPSADRWAQPAAADELTSL
                     DDHTLKVMGIARYEIPGIVAAEMAPQAAANEEDPPQPINARRAMTQSGHAPRGHAPSA
                     EESSMSRSLILHPASRTGQRSFPMPPVRAERKLAAILAADVTGYSRLMGADEEGALAR
                     LKAHRRELIDPKIEEHRGRIVKTTGDGILVEFPSVVDAVRCAVETQEGMVARNADVPQ
                     DCRIDLRVGVNLGDIIVEGDDIHGDGVNIAARLEGLAEPGGICISQTVLNLVSGKVPF
                     EVEDAGEQALKNIDRPVHVYRINMGSAARRLASPEQALPLPDKPSIVVLPFVNLSGDP
                     EQEYFADGMVEDIITALSRMRWLFVIARNSSFAYKGRAVDARQVARELGVRYVLEGSV
                     RKTANRLRIAGQLIDGSTGAHLWADRFEGALEDIFDLQDQMTASVVGAIAPRLEQAEI
                     ERAKRKPTESLDAYDCHLRGMASLYQWTKEGTSEALRMFYRAIELDPDFASAYGTAAW
                     CYVLRESYGWMTDRAEEIAETARLARRAVELGREDAAALSTSGMALAYVVGDLDDGAA
                     FIDRALALNPNLAAAWYYSGWARIWLGEPDSAIEPISRAMRLSPLDPLINRMH"
BASE COUNT          403 a          668 c          689 g          351 t
ORIGIN      
        1 cccgcggccc cgacgacgtc atttccagga ccagatcgct cgaaagcccg cccggcgcca
       61 tccgattagc gtaggcggca agcccgccga agagcaacat ttgcacccag gggaagcggt
      121 gcagcctgac gccgatgccc cgctccatca cgggcacagc caagcatgtg tgaactagtt
      181 cacacccgca tgcggtgagg cgtgctagtt gcgtgttcca cgcaatgcgc ccgcgtgcac
      241 tttcgaggac gacaatgacg atgactgcgg acaacaacag taacagtgct aagaccgtgg
      301 gcggaggcct gttcaaccga tacatcttga agccatccgc cgatcggtgg gcgcagccag
      361 ctgccgccga cgagctgacg tcgctcgacg accacacact caaggtcatg ggcattgcac
      421 gctacgagat tcccggaatc gttgctgccg agatggcgcc gcaggcggct gcgaacgaag
      481 aggacccacc gcaaccgata aacgcgagac gcgctatgac ccaatccgga cacgcgcctc
      541 gagggcacgc accctccgct gaagagtcct ccatgagccg atccctgatt ttgcatccgg
      601 cttcacgcac cgggcagaga agttttccta tgccgccagt gcgcgcggaa cgtaagctgg
      661 cagctatcct cgctgccgat gtgactggct acagccggct catgggcgcg gacgaggaag
      721 gcgctctcgc gcgcctcaag gctcaccgcc gcgagctgat cgatcccaag atcgaggaac
      781 accgcgggcg catcgtcaag acgactggcg acggcatcct ggtcgagttt ccctccgtcg
      841 tggatgcggt gcgctgcgcc gtcgagacgc aggaaggcat ggtcgcccgg aacgccgatg
      901 tcccgcagga ctgtcggatc gacttgcggg tcggcgtcaa cctcggggac atcatcgtcg
      961 aaggcgacga catccatggc gatggcgtca atattgcggc tcggctcgaa gggttggcgg
     1021 aacctggagg tatctgcata tcgcagacgg tcctcaatct cgtgtcgggc aaggtgccgt
     1081 tcgaagtcga ggatgcgggc gaacaagcgc tgaagaacat cgatcggccc gtccatgtct
     1141 atcgcatcaa catggggtcg gccgcgcggc gccttgcatc tccggagcaa gccttacccc
     1201 tccccgacaa gccgtcgatc gtcgtgctgc ctttcgtcaa cttgagcggc gatccggagc
     1261 aggagtactt cgccgacggc atggtggagg acatcatcac ggcgctgtcc cgcatgcgct
     1321 ggctgttcgt gatcgcgcgc aactcgagct tcgcctacaa gggccgcgcc gtggacgcga
     1381 ggcaggtggc gcgtgagctg ggtgtccgtt acgtgctcga aggcagcgtc cgcaagaccg
     1441 caaatcgcct acgcatcgcc gggcagctca tcgatggctc gaccggcgcg catttgtggg
     1501 cggaccgctt cgagggcgcg ctcgaggaca tcttcgattt gcaggaccag atgacggcga
     1561 gcgtggtggg tgcgatcgcc ccgaggctgg agcaggcgga gatcgaacgc gccaagcgca
     1621 agccgaccga gagcctcgac gcctacgact gccatttgcg cgggatggcc agcctttatc
     1681 agtggaccaa ggagggcacg agcgaggcgc tgcggatgtt ctatcgggcg atcgagctcg
     1741 accctgactt cgcgtcagca tatggcacag ccgcgtggtg ctacgtcttg cgcgagtctt
     1801 acggctggat gaccgaccgc gcggaagaga ttgccgaaac cgcaaggctg gctcggcggg
     1861 cggtggagtt gggcagagaa gatgcggcag cgctctccac cagcgggatg gcgctcgcct
     1921 atgtcgtcgg cgacctcgac gacggcgcgg ccttcatcga tcgggcgctc gcgctcaacc
     1981 cgaatctggc agcggcgtgg tattacagcg gctgggcgag aatatggctc ggcgagccgg
     2041 actcggccat cgagcccatt tcgcgcgcta tgcgtctgag cccactcgat ccgctgatca
     2101 atcgcatgca c
//
LOCUS       DATLKL010000238         3249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_171888, whole genome shotgun sequence.
ACCESSION   DATLKL010000238 DATLKL010000000
VERSION     DATLKL010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171888"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VM713_03100"
     CDS             complement(<1..757)
                     /locus_tag="VM713_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVH83283.1"
                     /translation="MKANTDSFVDSRDFRRIARAIRFIDAHFREQPRLATIAASAGLS
                     EFHFNRLFRRWAGVTPRQYLAFVTARAARGALAGATSVLEAAYAVGLSGPGRLHDLLV
                     TLDAVTPGELKTSGAGVEIRYGFSDTPFGTALIASTARGVCHLGFEDRPRERAAAAEL
                     AARWEQARLTRNDADAQRTALEIFGAGQHGDGAPIRLAVKGTNFQLKVWQALLEVASD
                     GPTTYRAVAESAGVPGAERAVGNAVAANPVAWLI"
     gene            complement(835..966)
                     /locus_tag="VM713_03105"
     CDS             complement(835..966)
                     /locus_tag="VM713_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83284.1"
                     /translation="MRRFLIGLTLALLLAVSIGVGVTVARWPHCRHVCPWLCVRQSV"
     gene            1002..2480
                     /locus_tag="VM713_03110"
     CDS             1002..2480
                     /locus_tag="VM713_03110"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HVH83285.1"
                     /translation="MSAAPEKLAICLLASEVAPLSKTGGLADVAGALAKYLHRAGHDV
                     RLFTPCYSSIDRAALAARPVAALQHVPLALGLHLYEFSVLEAPLPGADGARVFLIDCP
                     ELYARATLYTADPDEHLRFIALTRAALMSCALLEWVVQIVHCNDWHTAFAPLFLRTEY
                     ARVAPFAAARTVLTIHNIGYQGIFAAADIADLGLGSGAAPLLQQKDLAAGSINALRHG
                     ILYADAVTTVSPTYAREICTAEFGMGLEDSLRERSGALTGILNGVDYDEWDPQRDRFL
                     PVHYDAEHLAGKAALKRQLLARARLTTAPEVPLAGVVSRLAAQKGIELMFEALPKVLS
                     ARPLAFAALGSGEAKYEQFFSTLARSFPGRVVFESGYDEELSHWIEAASDLFLMPSRY
                     EPCGLNQMYSLRYGTVPVVRRTGGLADSVEHFDPASGAGTGVVFNDFDAPALEWALNF
                     ALDLYAQPQHWSRMMRNGMSRDFSWQRATAEYLTVYRRLTAG"
     gene            complement(2467..2925)
                     /locus_tag="VM713_03115"
     CDS             complement(2467..2925)
                     /locus_tag="VM713_03115"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HVH83286.1"
                     /translation="MPRMRAIATATALALLSGGCALGPKLTTPKLTIVGVRLEGSDLL
                     AQHLRLRLHVQNPNDRTLPVKGIAYTIDVDGQPFASGESAASFVVPARGEAEFDMNVT
                     TNVASTLVRLLARGPDAPASVPYRLSGKISLSAGWLQSIPFEQRGSFNLQ"
     gene            complement(2942..>3249)
                     /locus_tag="VM713_03120"
     CDS             complement(2942..>3249)
                     /locus_tag="VM713_03120"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HVH83287.1"
                     /translation="GTHDNDTTLGWYSHLDAATRARVRLYLDAGAMPEALIRAALGSV
                     ARLAIVPVQDLLGLGSEARLNTPGTAGGNWTWRLPPAPLSADLARRFAELNAVYGRT"
BASE COUNT          505 a         1144 c         1102 g          498 t
ORIGIN      
        1 ggatgagcca tgcgaccggg ttggcggcga cagcgttccc cacggcccgc tcggcacccg
       61 ggactcccgc cgactcagcc accgcccggt aggtggtcgg cccatcggat gcaacctcga
      121 ggagcgcctg ccataccttg agctggaaat tcgtcccctt gaccgcgagc cgtatcgggg
      181 cgccgtcgcc gtgctggccg gcgccaaaga tctcgagcgc cgttcgctgg gcgtccgcgt
      241 cgttgcgcgt gaggcgcgcc tgctcccatc gggccgcgag ctcggcggcc gccgcgcgct
      301 cgcgcgggcg gtcctcgaac ccgagatgac agacaccgcg cgccgtgctc gcgatcagcg
      361 cggtgccgaa cggcgtgtcg ctgaagccat agcgtatctc gacacctgcg ccactggtct
      421 tgagctcacc cggtgtcacc gcatcgagcg tcacgagcag gtcgtgcagc cggccggggc
      481 ccgagagtcc cactgcatag gccgcctcga ggaccgatgt ggccccggcg agtgcgccgc
      541 gcgcggccct cgccgtgacg aatgccagat actgccgtgg ggtaacgccc gcccagcgcc
      601 ggaacagccg gttgaaatga aactcggaca gacccgcgga ggcggcgatg gtcgcgaggc
      661 gcggctgctc gcggaaatgc gcgtcgatga aacggatcgc acgcgcgatg cgccgaaaat
      721 cgcggctgtc gacgaagctg tcggtgttcg ctttcatgcg atgggatgat agccgcgcgt
      781 gggcggtccg cgacccgatt cttgcggccc gcaaagggcg tttccagcct tcggttacac
      841 tgactgccgc acgcacagcc agggacacac gtgccggcag tggggccagc gggccaccgt
      901 cacgcccacc ccgatcgaga ccgcgagcag cagtgccagc gtgagcccga tcaggaagcg
      961 tcgcatgcgc caatcttaac ctgacaccga acagccaaat aatgagcgcc gcgccggaaa
     1021 aactcgcgat ctgcctgctc gcctccgaag tggcgccgct gtctaagacc ggcggactgg
     1081 ccgacgtcgc cggcgcgctc gccaaatacc tgcaccgcgc cgggcacgac gtgcggctct
     1141 tcacgccttg ctattccagc atcgatcggg cggcactcgc cgcgcggcca gtcgccgcgc
     1201 tgcagcacgt gccgctcgcc ctgggtctgc atctctacga gttctcagtg ctcgaggcgc
     1261 cactccccgg agccgacggc gcccgggtgt tcctcatcga ctgcccggag ctgtacgcac
     1321 gcgcgaccct gtacacggcg gatcccgatg agcacctgcg ctttatcgcc ctgacgcgcg
     1381 cggcgctcat gagctgcgcg ctgcttgagt gggtcgtgca gatcgttcac tgcaacgact
     1441 ggcacacggc attcgcgccg ctgttcctgc gcaccgagta cgcccgcgtg gcaccgttcg
     1501 cggcggcgcg caccgtgctc acgattcaca acatcggcta tcagggaatc tttgccgccg
     1561 ccgacatcgc cgacctgggc ctcggctccg gcgccgcgcc cctgctccaa cagaaggacc
     1621 tcgccgccgg aagcatcaat gcgctgcgcc acgggatcct ctacgccgat gccgtcacca
     1681 ccgtgagccc gacctatgcg cgcgagatct gcaccgccga gttcggcatg ggactcgagg
     1741 acagcctgcg cgagcgcagc ggcgcgctca ccgggatcct caacggcgtc gattacgacg
     1801 aatgggatcc acagcgcgac cgctttctgc cggtgcacta cgacgccgaa catctcgccg
     1861 gcaaggcggc gctcaagcgg cagctgctcg cgcgggcgcg cttgacgacg gcgcccgagg
     1921 ttccgctcgc gggtgtcgtg agccgcctcg ccgcgcagaa gggcatcgag ctcatgttcg
     1981 aggcactgcc gaaggtgctg agtgcgcgcc cgctggcatt cgccgcgctc ggcagcggcg
     2041 aggcgaagta cgagcagttc ttcagcacgc tcgcgcgctc cttcccggga cgcgtggtgt
     2101 tcgagagcgg ctacgacgag gagctctcgc actggataga ggcggcgagc gacctgtttc
     2161 tcatgccctc gcgctacgag ccctgcggcc tgaaccagat gtacagcctg cgttacggca
     2221 ccgtgcccgt cgtgcgccgc accggcgggc tggccgactc ggtcgagcac ttcgatcccg
     2281 caagcggcgc cggcacgggc gtcgtgttca acgacttcga cgcgccggcg ctcgagtggg
     2341 cgttgaactt cgcgctcgat ctgtacgcgc agccgcagca ctggtcgcgc atgatgcgca
     2401 acggcatgag tcgggacttc tcctggcagc gcgcgaccgc ggagtacctc acggtctacc
     2461 ggcggctcac tgcaggttga agctgccgcg ctgctcgaag ggaatcgact ggagccagcc
     2521 cgccgacagc gagatcttgc cgctcagccg ataggggacg ctcgcgggcg cgtcgggtcc
     2581 gcgcgcgagc agccgcacca gcgtgctcgc cacgttggtg gtcacgttca tgtcgaactc
     2641 ggcctcgccg cgcgccggca ccacgaagct cgccgccgac tcgccgctag cgaacggctg
     2701 cccgtcgacg tcgatcgtgt acgcgatccc cttgacgggc agcgtacggt cgttggggtt
     2761 ctgcacgtgc aggcgcaacc gcaggtgttg cgcgaggagg tcactcccct cgagccgtac
     2821 tccgacgatg gtgagtttgg gagtcgtgag cttcgggccc agcgcacagc caccactgag
     2881 cagcgcgagc gcggtggcag tggcgatggc tctcatgcgc ggcatgttag ttcgcttcat
     2941 actaggtgcg cccgtagacg gcattgagct cggcgaagcg gcgcgcaaga tctgcgctaa
     3001 gtggggcggg cggcagccgc caggtccagt tgccacccgc cgtgccgggc gtgttgagac
     3061 gcgcttcgga gccaagaccc aggaggtcct gcaccgggac gatcgcgagc cgcgccaccg
     3121 agccgagcgc ggcgcggatc agcgcctcgg gcatcgcacc cgcgtcgaga tagagccgca
     3181 cgcgggccct ggtcgccgcg tcgaggtgcg agtaccagcc gagcgtcgtg tcgttgtcgt
     3241 gggtgccgg
//
LOCUS       DATLKL010000239         3595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_36671, whole genome shotgun sequence.
ACCESSION   DATLKL010000239 DATLKL010000000
VERSION     DATLKL010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36671"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..708
                     /locus_tag="VM713_03125"
     CDS             328..708
                     /locus_tag="VM713_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83288.1"
                     /translation="MKQRKVSTDSDPGPRLQRLNEYLERNFPDFFAEARFQVGDDDYF
                     LYARFGQYLARTIEQNRASGKVINRGFTLLNRMARASARSPSIRQMLVSGPLEYIVEA
                     PKARALAQKRLSAEAQGYLESLCE"
     gene            complement(756..1778)
                     /locus_tag="VM713_03130"
     CDS             complement(756..1778)
                     /locus_tag="VM713_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVH83289.1"
                     /translation="MPALMLDEHAYGADAGSPQGNAPAETAAPPEPLDAYSAAVVGAV
                     ERVGPAVVSVYTGGAEEAAQARGGAGSGVVVTPDGYLLTNEHVVQRVQEARVSFVDGR
                     TVAATVAGRDPATDLAVLRAQAASLPYAQLASTQRLRAGQLVVAVGNPYGFESTVSAG
                     VVSALGRSLRSRQGRLIEGIVQHTAALNPGNSGGPLVDAHGAVTGINTAIIPMAQGIG
                     FAIPAATAQWVLTQILTQGRVRRAWLGIAARDRPLDLRLVRALDLRARRAVEILSRER
                     EGPAAESDLQPGDLIVGVNDDPVDGIDTLHRLLGGVPPGTEIALTVVRRARRLTVPLT
                     AREPPG"
     gene            1844..>3595
                     /locus_tag="VM713_03135"
     CDS             1844..>3595
                     /locus_tag="VM713_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HVH83290.1"
                     /translation="MISLAETAILLAAAVVAVSLFRFLRLSSILGYVAAGLVIGPWGL
                     NLVGDVDRITKASEFGIVLLLFIIGLELQPTRLWVMRRTVFGLGAAQVTLCTVLLGAA
                     AWSVGLPPVAAGVVGFGLSLSSTPLVLQLLAERGELKAQHGRAAFSILLFQDLAVLPV
                     LAILPMLSPGGASHPAGGAWWTGLLKLLVVIAGVIVGGGRLLLRPALRIVARVRVTEV
                     FTAAALLTVIATALIANQAGLSMSLGAFLAGVLLADSEFRHELEADLEPFKGLLLGLF
                     FVCVGMSANLGLLRSEPLTLLAVTVAFLAIKVLAVAAVGRFGRCRGDEAWKLGFTLPT
                     GGEFAFVLFTLASRQRLIDGPTADLLVLAVTLSMMLAPLLLLAQEAVSARWLAPARAP
                     YDAIDERDTPVIIAGFGRFGQIVARVLQVQRLSFTALDSSQTHVDFVRRFGNKVYYGD
                     ASRLDLLRAAGAATARVLVLALDDVEASVRAAVLAREQFPNLRIFARARSRQHAFALM
                     DAGVTVIVRETYAASLEMAGRVLEAVGKTPAAARETVRRFREHDERTLAAQYAVKEDE
                     SKFRATTVEAAQQLEKLF"
BASE COUNT          497 a         1272 c         1226 g          600 t
ORIGIN      
        1 ccatctgtct cacagtcgca ggccgatccc gacgtcgaga aacggctcca gctgccgccg
       61 ctggtagggc gcgaaatgca gatccggcgg cgagatccac cgggagcccc gttgctgcgt
      121 gcgtgatgac cgcatcaaac ccgtagtagt cgaggtcgtc gtcgccgaca ccgctcggga
      181 tcccgcgggg cggccggagc ctcgccgcga aactcctcac tcgacgagcg tcacaaccct
      241 catcacgacg cttgcgtaac gccgagctct gctccaacat tcgcgccgcc gcttactttc
      301 ggcggcgagc gaggtgttgg aacagcaatg aagcagcgca aagtgtcgac tgacagcgac
      361 cctgggcccc gtcttcaacg tctcaatgag tatctcgaga ggaatttccc ggactttttc
      421 gcggaagcgc gcttccaggt cggggatgac gactactttc tctacgcccg tttcgggcag
      481 tacctggcgc gcaccatcga gcagaaccgc gcctccggca aggtgatcaa ccgcggcttc
      541 acgctcctca accgcatggc acgcgcctcg gcgcgctcgc ccagcatccg ccagatgctg
      601 gtctcggggc cgctcgagta catcgtggaa gcgccgaagg cacgggcact ggcgcagaag
      661 cgtctctccg cggaagcgca ggggtatctc gagagcctgt gcgagtaggc caaaaccggc
      721 ctgttgggcc tgaaccagta gctcggttgg ccaatctaac caggcggctc gcgcgccgtg
      781 agcggcactg tgagacgccg cgcccggcgc accacggtga gcgcgatctc cgtccccggc
      841 gggacgcccc caagcaggcg gtgcaaggtg tcgatgccgt cgaccgggtc gtcgttcacg
      901 cccacgatga ggtcgccggg ctgcagatcc gactccgccg ccggtccttc gcgctcgcgt
      961 gacaggatct cgaccgccct tctggcgcgc agatccaggg cgcgcaccag acgcaggtcg
     1021 agtggccgat ctcgcgccgc aatcccgagc caggcgcgcc gcacgcggcc ctgggtgagg
     1081 atctgggtca gcacccactg ggcggtcgcc gccggaatcg caaagccgat gccctgcgcc
     1141 atcggaatga tcgccgtgtt gatgcccgtg acggcgccgt gtgcgtcgac cagcggaccg
     1201 ccggaatttc ccggattgag ggcggcagtg tgctgcacga tgccctcgat gaggcgcccc
     1261 tggcggctgc gcagtgagcg gccgagcgcg ctcacgacgc ccgccgagac ggtggactcg
     1321 aatccatagg ggttgccgac agccacgacc agctgcccgg cgcgcaggcg ctgcgtgctc
     1381 gcgagctgcg cgtaagggag cgaggcggcc tgggcgcgca gcacggcgag atccgtggcc
     1441 ggatcgcgcc cggcaaccgt ggcggccacg gtgcgcccgt cgacgaatga cacgcgtgcc
     1501 tcctggacgc gctgcacgac atgctcgttg gtgaggagat agccgtcggg cgtcaccacg
     1561 acccccgagc ccgcaccgcc gcgcgcctgc gccgcctcct cggccccgcc ggtgtagaca
     1621 ctgaccaccg ccggccccac ccgctccacg gcgcccacga ccgccgcgga gtaggcatcg
     1681 agcggttcgg gcggagctgc ggtttcggcg ggtgcgttgc cctgaggcga cccggcgtct
     1741 gcgccgtacg cgtgctcgtc cagcatgagg gcaggcatgg ggacctgcgc cattaaactc
     1801 aagtccctgc acgggggatt cctgcgagaa tctccgcgca ccgatgatct ccctcgccga
     1861 aactgccatc ctgctggccg ccgcggtcgt cgcggtctcg ctgttccgct tcctgcgcct
     1921 ctcctcgatc ctgggctatg tggccgctgg cctcgtgatc ggcccttggg ggctgaatct
     1981 tgtcggcgat gtcgaccgca tcaccaaggc gtccgagttc ggcatcgtgc tgctgctctt
     2041 catcatcggc ctcgagctgc agccgacgcg gctctgggtc atgcggcgca ccgtgttcgg
     2101 cctcggcgcg gctcaggtca cactctgcac agtcctgctc ggagcggctg cctggtcggt
     2161 cggcctgccc ccggttgccg ccggcgtcgt cggcttcggg ctctcgctct cctcgacgcc
     2221 gctcgtgctg cagctgctcg ccgagcgcgg cgagctcaag gcccagcatg ggcgcgccgc
     2281 cttcagcatt ctcctcttcc aggacctcgc ggtgctgccg gtgctcgcga tcctgccgat
     2341 gctctcgccg ggcggcgcgt cgcacccggc cggcggcgcc tggtggacgg ggctgctcaa
     2401 gctcctggtg gtgatcgcgg gagtgatcgt cggcggcggc cggctgctgc tgcgccccgc
     2461 gctgcggatc gtggcacggg tgcgggtgac ggaggtgttc acggcggcgg cgctgctcac
     2521 cgtgatcgcg accgcgctga tcgccaatca ggcggggctg tcgatgtcgc tcggcgcctt
     2581 tctcgccggc gtgctcctcg ccgacagcga attccgccac gagctcgagg cggacctcga
     2641 gcctttcaag gggctgttgc tcggcctgtt cttcgtctgc gtcggaatgt cggcgaacct
     2701 cgggctgctc aggtccgagc cgctcacgct gctcgcggtc acggtcgcct ttctcgccat
     2761 caaggtcctg gccgtcgcgg ccgtcgggcg tttcggccgc tgtcggggcg atgaagcctg
     2821 gaagctcggc tttaccctcc cgaccggagg cgagttcgcc ttcgtgctgt tcacgctcgc
     2881 gtcaaggcag cgcctcatcg atggcccgac cgcagacctg ctggtgctcg cggtgacgct
     2941 ctcgatgatg ctcgcgccac tgctgctgct cgcgcaggaa gcggtgagcg cgcgctggct
     3001 cgccccggcg cgggccccct acgacgcgat cgacgagcgc gatacgccgg tgatcatcgc
     3061 cggcttcggc cgcttcgggc agatcgtcgc gcgcgttctg caggtgcagc gcctgtcctt
     3121 cactgcgctc gacagcagcc agacccacgt cgatttcgtg cggcgcttcg gcaacaaggt
     3181 gtactacggc gatgcctcgc ggctcgacct gctgcgcgcg gccggtgccg ccacggcgcg
     3241 cgtcctggtg ctggcgctcg atgacgtcga ggcttcggtg cgcgccgcag tgctggcgcg
     3301 cgagcagttt ccgaatctca ggatattcgc ccgtgcccgc agtcgtcagc atgcctttgc
     3361 gctcatggac gcgggcgtga cggtgatcgt gcgcgagacc tacgccgcga gcctcgagat
     3421 ggccggccgg gtgctcgagg cggtcggcaa gacccctgcg gccgcgcgcg agacggtacg
     3481 gcgcttccgc gagcacgacg agcggacgct cgccgcgcag tatgcggtca aggaggatga
     3541 gagcaagttc cgcgccacga cggtcgaagc cgcccagcag ctcgagaagc tgttc
//
LOCUS       DATLKL010000240         2292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_59130, whole genome shotgun sequence.
ACCESSION   DATLKL010000240 DATLKL010000000
VERSION     DATLKL010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59130"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VM713_03140"
     CDS             complement(<1..185)
                     /locus_tag="VM713_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83291.1"
                     /translation="MKTAVPPGARRAALVRIDRLGAVLVAAGAAALLCLPFVLFKSNR
                     IVPGDPRSLMQVLPIWGA"
     gene            complement(182..1093)
                     /locus_tag="VM713_03145"
     CDS             complement(182..1093)
                     /locus_tag="VM713_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HVH83292.1"
                     /translation="MRQLLKSLCVLAVLSLGVPAAAPAAVVVSSKLSSESAMIGEMIR
                     LLLDAHGVPTVDRMTLGATPVVRKALLSGEIDLYVEYTGNAGFFFNDPGNPAWKDLRK
                     GYELGARLDYAANHIVWLTPAEASNAWALAVRRDVAAEQQLRTMSDFARWVASGGRVV
                     LACSAEFANAGTLRSLERTYGFTLRSDQMIVLAGGETSATITAAAARTNGINTAMVYG
                     TDGGIVAANLVMLEDDRHDQPVYAPVPTMREAVLKEYPQVAAIVKPLMESFDRETLQQ
                     LNARVQVNGESAESVAQDYLRTRGLLK"
     gene            1158..>2292
                     /locus_tag="VM713_03150"
     CDS             1158..>2292
                     /locus_tag="VM713_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HVH83293.1"
                     /translation="MPTPPRSPAPAARPGRPSSGRYSLWRLLREGVTGQRGWSPAWRK
                     ATPRARYRVVVVGGGGHGLATAFYLATEHGIRDVAVLERGPIGLGNVGRNTTIIRSNY
                     FQPANVRFYEFSLKLWERLERTLNFNTMVSQRGTLNLFHTEAQRDLFTRRANLMRLEG
                     VDAEALSREAVKALVPLVDLDNGRYPVLGGFLQPRGGTVRHDAVAWGYARAASEAGVD
                     IVEHCVVTGIGQQDGRVTGVETTRGFIAADTVCLAMAGHTSELARMAGIELPIETHVL
                     QAFVTEGLKPVLNTVLTYGAGHFYVNQSDKGGLVFGGAIDGYNTYGQRGTLPMVEEVT
                     AAAVTLLPALARVKVLRQWGGVMDMTMDVSPIISATPLAGLILN"
BASE COUNT          361 a          789 c          779 g          363 t
ORIGIN      
        1 gccccccaga tgggcaacac ctgcatgaga ctgcgtgggt caccgggcac gatacggttc
       61 gacttgaaga ggacgaacgg caggcacagg agcgccgcgg cgccggccgc gaccagcacc
      121 gcgccgagcc ggtcgatgcg gacgagggcc gcacgcctcg cgcccggcgg caccgccgtc
      181 ttcacttgag gaggccccgc gtgcgcaggt agtcctgcgc gaccgactcg gccgactcac
      241 cattcacctg cacgcgggca ttcagctgct gcagcgtctc gcggtcgaag ctctccatca
      301 gcggcttgac gatcgctgcg acctgtggat attccttgag cactgcctcg cgcatcgtcg
      361 gtaccggcgc atacaccggc tggtcgtgcc ggtcatcctc gagcatgacc aggttggcgg
      421 cgacgatgcc gccgtcggtg ccgtacacca tggcggtgtt gataccgttg gtgcgcgccg
      481 ccgccgcagt gatggtggcg gaggtctcac cgcccgcgag cacgatcatc tggtcggagc
      541 ggagagtgaa gccgtaggtt ctttcgagcg agcgcagcgt gccagcgttg gcgaactccg
      601 ccgagcaggc aagcaccacc cggcctccgc tcgcgaccca gcgtgcgaaa tcgctcatgg
      661 tgcgaagctg ctgttccgcg gcgacgtcgc gccgcaccgc gagcgcccag gcgttgctcg
      721 cctcagcggg cgtcagccac acgatgtggt tggccgcgta gtcgagccgc gcgccgagct
      781 cgtaaccctt gcgcaggtcc ttccaggccg gattccccgg gtcgttgaag aagaagcccg
      841 cgttgccggt gtactcgacg tagagatcga tttcgcccga gagcagcgcc ttgcgcacca
      901 ccggggtcgc gcccaaggtc atgcggtcga cggtcggcac gccgtgcgcg tcgagcagca
      961 ggcggatcat ctcgccgatc atcgccgatt cgctcgagag cttggaggag acgacgaccg
     1021 ctgccggcgc ggcagccggc acgcctagcg acagcacggc gagcacgcac agcgacttga
     1081 gtaactgcct cacccggaca cgttgtcggt tgccgtcaag cgcgtcaagg gccgcacccc
     1141 ttatcatgcc gccaccgatg ccgactccgc cgcgctcacc cgcaccagcg gcccgcccag
     1201 ggcggccctc gagcggccgc tattcgctct ggcggctgct gcgcgagggt gtgacgggac
     1261 agcgcggctg gtcgcccgcc tggaggaagg cgacgccgcg cgcgcgctat cgcgtcgtcg
     1321 tggtcggcgg cggcggccat gggctcgcca ccgcctttta tctagcgacc gagcacggca
     1381 tccgcgacgt cgcggtgctg gagcgcggcc cgatcggcct cggcaacgtc ggccgtaata
     1441 ccaccatcat ccgctcgaac tacttccagc ccgccaacgt gcgcttctac gaattctcgc
     1501 tgaagctgtg ggagcgtctc gagcgcacac tcaatttcaa cacgatggtg agccagcgcg
     1561 gcacgctcaa cctgtttcac accgaggcgc agcgcgatct tttcacccgc cgcgcgaacc
     1621 tgatgcgcct cgagggagtg gatgcagaag cactgtcgcg cgaggcggtc aaggcgctcg
     1681 tgccgctcgt cgatctcgac aatggccgct acccggtgct cggcggcttc ctgcagccgc
     1741 gtggcggcac ggtgcgccac gatgcggtcg cctggggcta tgcgcgcgcc gcttcggagg
     1801 cgggggtcga catcgtcgag cactgcgtgg tcaccgggat cgggcagcag gacggacggg
     1861 ttacgggcgt cgagaccacg cgcggcttca tcgccgccga taccgtgtgc ctggctatgg
     1921 ccggccatac ctccgagctc gcccgcatgg ccggcatcga gctgccgatc gagacccacg
     1981 tgctgcaggc cttcgtgacc gagggcctca aaccggtact caacaccgtg ctgacctacg
     2041 gcgccggtca tttttatgtc aaccagtccg acaagggtgg actggtgttc ggcggggcca
     2101 tcgacggtta caacacctac ggccagcgcg ggacgctgcc gatggtcgag gaagtgactg
     2161 ccgcggcagt gacgctgctg ccggcgctcg cgcgggtgaa ggtgctgcgg cagtggggcg
     2221 gggtcatgga catgacgatg gacgtctcgc cgatcatctc cgcgacgccg cttgcggggc
     2281 tgatcctaaa cg
//
LOCUS       DATLKL010000241         1676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_81623, whole genome shotgun sequence.
ACCESSION   DATLKL010000241 DATLKL010000000
VERSION     DATLKL010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81623"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VM713_03155"
     CDS             complement(<1..220)
                     /locus_tag="VM713_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83294.1"
                     /translation="MNDILVPVSPGELLDKITILRIKAARILDAGKLRNVKTELALLE
                     STWRDSGCADRGLAQEERALQRVNEQLRD"
     gene            complement(217..1269)
                     /locus_tag="VM713_03160"
     CDS             complement(217..1269)
                     /locus_tag="VM713_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HVH83295.1"
                     /translation="MLPLAEPPRSLCILRLSAIGDTCHVVPIVRTLQQAWPATQLTWI
                     IGRTEARLMSLIEGVEFITVDKGAGLAVRRALRERLRGRRFDVLLHMQLALRASLVSL
                     AVPADLKLGFDRPRARELQWLFTNERIAAGTREHVLDSFFGFPAALGIRERLLRWDVP
                     LPAAARAYAERLIPDAQPTLIISPCSRHARRNWPLERYAALARHAAGAHRMRVILAGG
                     PSEAERRAGAAIARAAGTAVLDQTGQDTLPEMLALLARATVLVSPDSGPVHMATMVGT
                     PVIGLYAATNPARSGPYLSGRWCVDAYARAARAFRGCEPQELPWGHKIEEPGVMDLIQ
                     VEEVTARIDELLGSRT"
     gene            1366..>1676
                     /locus_tag="VM713_03165"
     CDS             1366..>1676
                     /locus_tag="VM713_03165"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HVH83296.1"
                     /translation="MSNLTAEWFAPQYWQSQGTLEASASGRGAAWFLSAGAKRYVLRH
                     YRRGGLAARLSSDHYAYLGEDNTRPFAEWQMTYSLHRAGLPVPAPVAARYRRVGLSYT
                     G"
BASE COUNT          243 a          581 c          602 g          250 t
ORIGIN      
        1 tatcccgcag ctgctcgttc actcgctgca gcgcccgctc ctcctgggcg aggccgcggt
       61 cggcgcagcc cgagtcgcgc caggtcgact cgagcagtgc cagctcggtc ttcacgttcc
      121 tgagctttcc cgcatcgagg atgcgggccg ccttgatcct caggatcgtg atcttgtcga
      181 gcagctcgcc cggggagacg ggcacgagta tgtcgttcat gtgcgtgatc ctagcaactc
      241 gtcgatccgg gcggtgacct cctcgacctg gatgaggtcc atgacgcccg gctcctctat
      301 cttgtgcccc cagggaagct cctgcggctc gcagccgcgg aaggcgcgcg ccgcacgggc
      361 gtaggcatcg acgcaccagc gccccgagag ataagggccg ctgcgtgcgg ggttggtcgc
      421 ggcgtacagc ccgatcaccg gagtgccgac catggtcgcc atgtgcacgg gacccgagtc
      481 gggacttacc agcacggtgg cgcgcgcgag cagcgccaac atctcgggca gcgtgtcctg
      541 cccggtctga tcgagcaccg cggtgccggc cgcgcgggcg atcgcagcgc cggcgcgccg
      601 ctcggcctcg ctcggtccgc cggcgagtat gacgcgcatg cgatgtgctc cggcggcgtg
      661 tcgggccagt gccgcgtagc gctcgagcgg ccagttgcgc cgcgcatggc gcgagcaggg
      721 gctgatgatg agggtcggct gcgcgtccgg tatcagccgc tcggcgtagg cgcgcgccgc
      781 cgccggcagc ggtacgtccc agcgcagcag gcgctcgcgg ataccgagcg ctgcggggaa
      841 gccgaagaaa ctgtcgagaa cgtgctcgcg ggtgccggcg gcgatgcgct cgttggtgaa
      901 cagccactgc agctcgcggg cgcgggggcg atcgaagccg agcttgaggt ccgccggcac
      961 cgcgagcgat accaggctcg cgcgcagcgc cagctgcatg tgcagcagca cgtcgaagcg
     1021 gcggccgcgc agccgctcac gcaacgcacg ccgcacggca agtcctgcac ccttatccac
     1081 ggtgataaac tcgacgccct cgatgaggct catcaggcgc gcttcggtcc gcccgatgat
     1141 ccaggtcagc tgcgtcgccg gccacgcctg ctgcagggtg cgcacgatgg gcacgacgtg
     1201 gcaggtatcg ccgatcgccg acagcctgag gatgcagaga ctgcgcggcg gttcggcgag
     1261 aggcagcatg atggagtcag acctgtgaat gggcgttggg cgctcggtcc tgaggtaagg
     1321 cgcggcgccc tcgcgcgcgg cgccatgcta tatgacgcct cccgcgtgag caatttgacc
     1381 gccgagtggt ttgcgccgca gtactggcag tcgcagggca ccctcgaggc cagcgccagc
     1441 gggcgcggcg ccgcgtggtt cctgagcgcc ggcgccaagc gttacgtgct gcgccactat
     1501 cgtcgcggcg ggctggcggc gcgcctctcc agcgaccact acgcctatct cggcgaggac
     1561 aacacgcgcc cgttcgccga gtggcagatg acctacagcc tgcatcgcgc cggactgccg
     1621 gtgcccgcgc cggtggccgc gcgctatcgc cgcgtcggcc tgagctacac cggcga
//
LOCUS       DATLKL010000242         1894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_160652, whole genome shotgun sequence.
ACCESSION   DATLKL010000242 DATLKL010000000
VERSION     DATLKL010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160652"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..>1894
                     /locus_tag="VM713_03170"
     CDS             58..>1894
                     /locus_tag="VM713_03170"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83297.1"
                     /translation="MSMYFHRCGVIVLGCALVLLTALAAADTDGPALFGALKWRSIGP
                     YRGGRALAVAGIPGDPDTFHFGAVAGGVWKTTDAGATWQPLTDGTPISSVGALAIAPS
                     DHKVIYVGTGEAAPRGDMTYGDGVYKSVDGGKTWSHIGLTDSRQIGALIVDPRSADTV
                     LVAAFGHAFGPNTERGVYRTTDGGKSWTRVLYHDEQTGAIDVSFDPNNPSIVYAALWQ
                     ARRQPWNFSSGGPGSGLYRSTDGGVSWARLTGNGLPDGILGRIHVSVSPADSQRIYAM
                     IEAHAGGLYRSDDGGAHWARVSDDGRLTQRAWYFSTVLADPKHADTVYAENTGLFRST
                     DGGKTFELLPARHGDHHGLWIDPSDPDRLIESSDGGASISLDHGKTWTTQANQPTAAF
                     YHVAVDNRFPYYLYGAQQDLGSVAIASMDDEGAIVARDWYDVAGGECGFVIPDPRDAE
                     ITYGSSENFVGRLDRHNMQQQVIPVWPVDASGHAARDFEHRFNWTSPLTMSPFDPDTL
                     YYGMERLYKTRNRGGSWRAISPDLTRNDKSRQQASGGPVTKDITSVEYYDTIFAIAES
                     PLRAGLVWVGTDDGLIQLTHDDGGSWTNVTPSAMPAWSTIGMIEPS"
BASE COUNT          321 a          653 c          627 g          293 t
ORIGIN      
        1 ctagcgagaa cgtgtagaga aagactgtga aagcggccgc ggcggccgtg tgaagggatg
       61 agcatgtatt ttcatcgctg tggggtaatc gtcttgggct gcgcgctcgt gcttcttacg
      121 gcgctggctg ccgctgacac cgacggcccg gcgctcttcg gcgcgctcaa atggcgcagc
      181 atcggccctt atcgcggcgg ccgcgcgctc gccgtcgccg ggattcccgg cgatccggac
      241 accttccatt tcggcgccgt ggccggcggg gtctggaaga cgaccgacgc aggcgccacc
      301 tggcagccgc tcaccgacgg tacgccgatc tcatcggtcg gcgccctcgc gatcgcgccg
      361 tccgatcata aggtgatcta cgtgggcacc ggcgaggcgg caccgcgcgg cgacatgact
      421 tacggcgatg gcgtctacaa gtcggtcgat gggggcaaga cctggagcca catcgggctc
      481 accgacagcc gccagatcgg ggcgctgatc gtcgatccac gcagcgccga tacggtgttg
      541 gttgccgcct tcggccatgc cttcggcccc aacacggagc gcggcgttta ccgcaccacc
      601 gacggcggca aatcctggac gcgggtcctc tatcacgacg agcagaccgg tgcgatcgac
      661 gtgtcgttcg atcccaacaa tccaagcatc gtctacgccg cactgtggca ggcgcgccgt
      721 cagccgtgga acttctcgag cggcggaccg ggcagcgggc tgtaccgttc gaccgacggc
      781 ggcgtcagct gggcgcggct caccggcaac ggcctgccgg acgggattct cggccgcatc
      841 cacgtctcgg tctcacccgc cgactcgcag cgcatctacg cgatgatcga ggctcacgcg
      901 gggggcctct atcgctccga cgacggcggc gcgcactggg cccgtgtcag cgatgacggc
      961 cggctcacgc agcgggcctg gtacttctcc acggtcctcg cggacccgaa gcacgccgac
     1021 acggtgtacg cggagaacac cgggctcttt cgctcgaccg acggtggcaa gaccttcgag
     1081 ctgctgccgg cgcggcacgg cgaccatcac ggcctgtgga tcgacccgag cgacccggat
     1141 cgcctgatcg agtcgagcga cggcggcgca tcgatctccc tcgatcacgg caagacctgg
     1201 acgacccagg cgaatcagcc gaccgcggcg ttctatcacg tcgcggtcga caaccgcttt
     1261 ccctattacc tctatggcgc gcagcaggac ctcggcagcg tcgcgatcgc cagcatggac
     1321 gacgagggcg ccatcgtggc gcgcgactgg tacgacgtgg cgggcgggga gtgcgggttc
     1381 gtgatccccg atccgcgcga cgccgagatc acctacggaa gcagcgagaa cttcgtcggc
     1441 cgcctcgacc ggcacaatat gcagcagcag gtcatcccgg tatggccggt cgacgcctcc
     1501 gggcatgcgg ccagggactt cgagcaccgc ttcaactgga cctcgccact caccatgtca
     1561 ccgttcgatc ctgacacgct ctattacggc atggagcggc tgtacaaaac gcgcaatcgc
     1621 ggcggcagct ggcgcgcgat cagcccggac ctgacgcgca acgacaagtc gaggcagcag
     1681 gcctcgggcg ggccggtcac caaggacatc accagcgtcg agtactacga cacgatcttc
     1741 gccatcgcgg agtcgccgct gcgcgccggt ctggtctggg tggggaccga cgacgggctg
     1801 atccagctga ctcatgacga cgggggcagc tggacgaacg tcactccctc cgccatgcct
     1861 gcctggagca cgatcggcat gatcgagccc tcgc
//
LOCUS       DATLKL010000243         1756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_216944, whole genome shotgun sequence.
ACCESSION   DATLKL010000243 DATLKL010000000
VERSION     DATLKL010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216944"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="livM"
                     /locus_tag="VM713_03175"
     CDS             <1..889
                     /gene="livM"
                     /locus_tag="VM713_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012614404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HVH83298.1"
                     /translation="YVMLGWGLNIVVGLAGLLDLGYVAFYAVGAYSFALLARDLGVGF
                     WTSLPIAGALAALCGVVLGFPVLRMRGDYLAIVTLGFGEIIHIILLNWVSLTEGPNGI
                     IGIPRPTLFGWAFEGGEGNGGVHAPGWLPPRMIILFYIILALALLANAFTLRVRQLPL
                     GRAWEALREDEDACRALGLNPTTLKLSAFGIGALFGGLAGCFFATRQGFISPESFTFV
                     ESATVLAIVVLGGMGSQIGVAAAATLLVLLPELGREFEQYRMLLFGAAMVAIVVWRPG
                     GLVRRRRPTLLLGDREERA"
     gene            complement(901..1560)
                     /locus_tag="VM713_03180"
     CDS             complement(901..1560)
                     /locus_tag="VM713_03180"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HVH83299.1"
                     /translation="MAVAAVAFGVEEQTARAARLPLIGGALCLDFANTVSGLREAEHE
                     REHLHAYGHLIAWAAHAGAIQTGEARPLLKRARDDDGAAARALHRALALRDTIHDIFA
                     AIARHASIPQRALDGFNAALADASRHARLLPAEEGFAWGWDDLTARLDSPLWPVVRSA
                     GDLLTTGKLDRIKQCPYPHCGWLFVDFSKNKSRRWCEMSVCGNRTKARRHYMREKQMG
                     A"
BASE COUNT          256 a          563 c          594 g          343 t
ORIGIN      
        1 ctatgtgatg ctgggctggg gcctgaacat cgttgttggc ctagccggtc tgctcgacct
       61 tggctatgtc gcgttctacg ccgtcggcgc ctactccttt gcgcttctcg cgcgcgactt
      121 gggcgtcggc ttttggacga gcctgccgat tgccggtgcg ctcgcggctc tctgcggcgt
      181 ggtgcttggc tttcccgtat tgcgcatgcg tggtgactat ctcgcgatcg tgacgctcgg
      241 gttcggcgag atcatccaca tcattctgct caattgggtc agcctcacgg aggggccgaa
      301 cgggatcatc ggcattccgc gcccgacgtt gttcggatgg gcgttcgagg gtggcgaggg
      361 aaacggcgga gtccatgcgc ccggctggct gccaccacgc atgatcatcc tcttctatat
      421 cattctcgcc ctggcactcc tggcgaacgc cttcaccctc cgcgtgcgac agctaccgct
      481 cggccgcgcc tgggaagcat tgcgcgagga cgaggacgct tgccgcgcgc tcggtctcaa
      541 ccccaccact ctcaagctct cggcgttcgg catcggggcg ctgttcggcg gcctcgctgg
      601 ctgcttcttc gcgactcggc agggtttcat cagccccgaa agcttcacct tcgtggaatc
      661 ggcgacggta ctcgcgatcg tggtgctagg cggaatgggg agccagatcg gcgttgccgc
      721 ggccgccacc ctgctcgtgc tgctgcccga gcttggccgc gagttcgaac agtaccgcat
      781 gctcctcttc ggcgctgcga tggttgcgat cgtcgtctgg cgccccggcg ggctggtgcg
      841 gcgccgccgg ccgaccctgc tcctgggtga ccgggaagaa cgcgcgtagg gaccggcgac
      901 tcacgccccc atctgcttct cgcgcatgta gtggcgacgt gccttggtcc gattgccgca
      961 cacgctcatc tcgcaccagc gccggctctt gttcttgctg aagtcgacga agagccagcc
     1021 gcaatgtggg tacgggcact gcttgatgcg gtcgagcttg ccggtggtga gaagatcgcc
     1081 ggccgaacgc acgacgggcc agagcggcga gtcgaggcgg gcagtgagat cgtcccagcc
     1141 ccaggcaaag ccctcctcag ccggaagcaa gcgtgcatgg cgcgaggcat ccgcgagcgc
     1201 ggcgttgaag ccgtccagcg cgcgctgcgg aatcgacgcg tggcgggcga ttgcggcgaa
     1261 gatgtcgtgg atggtgtcgc gcagcgcgag cgcgcggtgg agcgcgcgcg cggcggcgcc
     1321 gtcatcgtct cgtgcgcgct tgagtagtgg ccgcgcctcg cccgtctgga tcgcccccgc
     1381 gtgcgcggcc caggcgatga ggtgcccata ggcatgcaaa tgctcgcgct catgctccgc
     1441 ttcgcgcagg ccggagaccg tgttggcgaa atcgaggcaa agggccccgc cgatgagcgg
     1501 caaccgcgcg gcgcgtgccg tctgctcctc gacgccgaaa gcgaccgctg cgaccgccat
     1561 taggcaaacc ataattgctt tgggtggtta gagtaccatg ccgtggcgcc gcgaaccagc
     1621 gctcgttttg gctatatgca acccagcgca gctgagaggc gcaattgcgg cggatagggc
     1681 ggctcggcgt cttcatgctc ttatggtcaa gagccggtga atggttaagg taatttttaa
     1741 caattgcgcc tagcct
//
LOCUS       DATLKL010000244         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_36710, whole genome shotgun sequence.
ACCESSION   DATLKL010000244 DATLKL010000000
VERSION     DATLKL010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36710"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..490)
                     /locus_tag="VM713_03185"
     CDS             complement(92..490)
                     /locus_tag="VM713_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83300.1"
                     /translation="MPGARVAEFRGTVRCRRAPHGPLGLTLSGYTTEHPGEEVRLAFA
                     GAAPADLPERLEDAVIERSNSGEYQIVSGAHAWPVAARSVHLHREIADRFYQAIPPRP
                     APWRRRLLFSAVLGIARSRAGIALVRALRR"
     gene            complement(492..968)
                     /locus_tag="VM713_03190"
     CDS             complement(492..968)
                     /locus_tag="VM713_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVH83301.1"
                     /translation="MAVHSYSARIHWQRGTAAFTDRRYSRAHRWTFDGGIEVPASSSP
                     QVVRVPLSDEHAVDPEEALVAALSSCHMLFFLDFAATQGFRVDDYRDDAAGVMGKNQA
                     GRTAMVRVTLHPRVSFSGERLPTRADIEELHHRSHEACFIANSVTTTVDVEPVWET"
     gene            1014..2015
                     /locus_tag="VM713_03195"
     CDS             1014..2015
                     /locus_tag="VM713_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVH83302.1"
                     /translation="MESPGLEPRRDLGLTHATSLVVGVIIGTGIFLKAASMAQAVGTP
                     GLVLAAWVAAGLVAMLGALCFAELGALLPHAGGEYVYLSRAYGELPGFLFACNAFVLG
                     GAAVAAYGAAVAIFVSDIHRFDARWYQHTLHLGSTTYVLEFGMRQLIAVGVIGVFTVV
                     NCLGVMLGGRVQTALTAVKVAAILTVAIGVFAFSDVHDFANLRGEPGANNGGFAGFGG
                     AMFAALWAYSGWQYMAMAAGEVREPQRNVPRAIVGGALLVLALYLTVNTAYLYALPFW
                     QVASANSTAYPEAPSVATRAVQTFLGGRAAPIAALIFLISTIGSLNGGAEKRAGGGD"
BASE COUNT          311 a          733 c          747 g          358 t
ORIGIN      
        1 gctcatcgga gcgcgggcgc ccgtcgtaga ggaagacgag gagtacgggc tgagctcacc
       61 gagctcctga ggccgacagc aggggcgtca ttcaacgacg caacgccctc accagcgcga
      121 tccccgcgcg gctgcgggcg atcccgagca ccgcgctgaa gagcaggcgg cgcctccagg
      181 gcgcgggccg cggcgggatc gcctgataga agcggtccgc gatctcgcgg tgcaggtgca
      241 ccgagcgcgc cgcgaccggc caggcgtgcg cgccgcttac gatttggtac tcgccggagt
      301 tactgcgctc gatcaccgcg tcctcgagcc gctcgggcag atcggcgggc gccgctcctg
      361 cgaaggcgag gcggacctcc tccccggggt gctcggtggt gtagccgctc agcgtcagcc
      421 cgagcgggcc gtgaggcgcg cgccggcagc gcaccgtgcc gcggaactcc gccactctcg
      481 ctcccggcat cttaagtctc ccacacgggc tcgacgtcga ccgtggtggt taccgagttg
      541 gcgatgaaac acgcctcgtg cgatcggtgg tgcagctcct cgatgtcggc gcgggtcggc
      601 agccgctcgc cagaaaagct cacgcgtgga tggagcgtga cgcgcaccat cgcggtccgg
      661 cccgcctgat tcttccccat gacaccggcg gcatcgtcgc gatagtcgtc gacccggaag
      721 ccctgggtcg ccgcgaagtc caggaagaac agcatgtgac agctcgagag ggccgccacg
      781 agcgcctcct cgggatcgac cgcgtgctcg tctgaaagcg gcacgcgcac cacttgtggt
      841 gacgaggatg ccgggacttc gatgccgccg tcgaaggtcc agcggtgcgc gcggctgtag
      901 cgcctgtcgg tgaaggcggc cgtgccccgc tgccagtgaa tccgtgccga gtacgagtgc
      961 accgccatgg cttttcctcc acgcgcttgg gttggcgatt gtacgatacg gcgatggaga
     1021 gccccggcct cgagccccgc cgcgacttag gcctcaccca cgcgacctcg ctggtggtcg
     1081 gcgtcatcat cggcaccggc atcttcctca aggcggcgag catggcgcag gcggtgggaa
     1141 cgccgggcct ggtgctggcc gcgtgggtcg ccgcggggct ggtcgcgatg ctcggcgcgc
     1201 tgtgctttgc cgagcttggg gcgctcctgc cccacgccgg cggcgagtac gtgtacctga
     1261 gccgcgccta tggcgagctg cccggattcc tgttcgcctg caacgccttc gtgctcgggg
     1321 gcgcggcggt cgccgcctac ggtgcggcgg tcgcgatctt cgtgtccgat atccaccgct
     1381 tcgatgcgcg ctggtaccag cataccctgc acctcggcag cacgacctac gtgctcgagt
     1441 tcgggatgcg gcagttgatc gccgtcggcg tcatcggcgt gttcacggtc gtcaactgcc
     1501 tgggcgtcat gctcggcggg cgagtgcaga ccgcgctcac ggccgtcaag gtcgccgcga
     1561 ttctcacggt ggcgattggg gtgttcgcct tcagcgacgt gcacgacttc gccaacctgc
     1621 gcggcgaacc gggcgcgaac aacggcggct tcgcgggctt tggcggtgcg atgttcgccg
     1681 cgctctgggc ctacagcggc tggcagtaca tggcgatggc ggcgggcgag gtgcgcgagc
     1741 cgcagcgcaa tgtgccgcgc gcgatcgtgg gcggggcgct gctggtgctg gcgctgtacc
     1801 tcaccgtcaa caccgcctac ctctatgcgt tgcccttctg gcaggtcgcg agcgccaact
     1861 cgaccgccta cccggaggcc ccctcggtgg cgacgcgcgc agtgcagact ttcctcggcg
     1921 gacgcgccgc gccgatcgcg gccctcatct ttctgatctc gaccatcggc tcgctcaacg
     1981 gaggcgccga aaaacgggcg ggcggtgggg attgaaccac ctaaaactct aaagcccttg
     2041 ccgctagcgg cttctcatcg aacgacttag cccgttttcc ggaactgaat gagttggcca
     2101 agttggccaa ttgaacgcgc cggatggtcg aaacccatgc cgatcctct
//
LOCUS       DATLKL010000245         1893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_138319, whole genome shotgun sequence.
ACCESSION   DATLKL010000245 DATLKL010000000
VERSION     DATLKL010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138319"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VM713_03200"
     CDS             complement(<1..258)
                     /locus_tag="VM713_03200"
                     /inference="COORDINATES: protein motif:HMM:NF045234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83303.1"
                     /translation="MRSEPGTHALLRIAHPPVSASGGPLPVWAEAALARAPWVVVRRA
                     RWRDGLIPVGLRGFERAERLAAWLHPRAVLECLTPRQLAARA"
     gene            complement(248..1075)
                     /gene="mdcE"
                     /locus_tag="VM713_03205"
     CDS             complement(248..1075)
                     /gene="mdcE"
                     /locus_tag="VM713_03205"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR03134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HVH83304.1"
                     /translation="MSPGDTPALGLRWLEALTGSAGLLDAGPRTVRVADAPLGAEVAR
                     FIAVVPDPHNRFPRARAGEVGLDEALGLAQQVRRTIAEDAQRKPRAIVALVDVTSQAY
                     GRREELLGIHLAGAAAVDAYVSARLAGHPLIALLVGKAISGGFLTHGYQANRILALDS
                     PAVLVQAMSKQAAARVTRRTVEELEQLGERVAPMAYDVRSYAKLGLLHGLIGGVDAAA
                     PAPGDLERVRAELIAAVADARAAVRDLSARLTSAGALAGRAASIAVRQRLAEQWDAL"
     gene            complement(1072..>1893)
                     /locus_tag="VM713_03210"
     CDS             complement(1072..>1893)
                     /locus_tag="VM713_03210"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR03133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HVH83305.1"
                     /translation="ASFIELNARERARAILDPGTFRELAGPFDRLKSPWLPLQGIVAQ
                     ADDGVVIARGSIEGKSAVVASIEGAYQGGSVGEVSGAKLAGALELALRDCERGRPVRP
                     VLLLETGGVRLQEANLGLAAIAEVHAAIVALRRHVPVVAVVAGLVGCFGGMSLAAALC
                     SRLIMTRQGRLGMNGPEVIEQEAGLAELEASDRALIWSLMGGEQRHATGLADVLVEDD
                     AAAIGAEVRRAFVAEAAPPRSAQVEALVARLAGIDAEHPPEPAALRALWARGAGH"
BASE COUNT          267 a          735 c          674 g          217 t
ORIGIN      
        1 cgcgcgcgcg gcgagctgcc gcggcgtcag gcactcgagc acggcacgcg ggtgcagcca
       61 ggcggcgagc cgctcagcgc gctcgaagcc gcgtagtccg accggaatca ggccgtcgcg
      121 ccagcgggcc cggcgcacga cgacccaggg tgcacgcgcg agcgccgcct cagcccacac
      181 cgggagcggc ccaccactcg cgctcaccgg tgggtgcgcg atgcgcagca gcgcgtgcgt
      241 gccaggctca gagcgcatcc cactgctcgg cgaggcgctg ccgcacggcg atcgaggcgg
      301 cgcggcctgc cagcgcccct gccgaagtga ggcgcgccga cagatcgcgt accgccgcac
      361 gcgcatcggc gacagccgcg atcagctcgg cgcgcacgcg ttccagatcc ccgggcgcgg
      421 gggcggcggc gtcgacgccg ccgatcagcc catgcaggag cccgagcttc gcgtaggaac
      481 gcacgtcgta agccatcggc gccacgcgct cgccgagctg ctcgagctcc tcgaccgttc
      541 ggcgcgtcac gcgcgccgcc gcctgcttgc tcatcgcctg caccagcacg gcgggggagt
      601 cgagtgccag aatgcggttg gcctggtagc cgtgcgtcag gaagccgccc gagatcgcct
      661 tcccgacgag cagtgcgatg agcggatggc ccgcgagccg cgcactgacg taggcatcga
      721 cggccgccgc cccggcgaga tgaatgccga ggagctcctc gcgccggccg taggcctgac
      781 tcgtgacgtc gacgagcgca acgatcgccc gcggcttgcg ttgcgcatcc tcggcgatcg
      841 tgcgacgcac ctgctgcgcc aaacccaagg cttcgtcgag gccgacctcg cccgcgcgtg
      901 cgcgcggaaa gcgattgtgg ggatcgggga cgaccgcgat gaagcgcgcc acctcggcgc
      961 cgagcggcgc gtccgccacg cgcaccgtgc gcgggcccgc atcgaggagt cccgccgagc
     1021 ccgtcagcgc ctcgagccag cgcaggccga gcgcgggggt gtcgcccggg ctcaatgccc
     1081 tgccccacgg gcccagaggg cgcgcagcgc ggccggctcg ggcggatgct cggcgtcgat
     1141 cccggcgagt cgggcgacga gcgcctcgac ctgcgcactg cgcggcggcg ccgcttcggc
     1201 cacgaatgcg cggcggactt ccgccccgat cgccgcggcg tcgtcctcga cgagcacgtc
     1261 ggcaaggccg gttgcgtgcc gttgctcgcc ccccatcagg ctccagatga gcgcgcggtc
     1321 gctcgcctcg agctcggcga ggccggcctc ctgctcgatc acctccgggc cgttcatccc
     1381 gagtcgcccc tggcgcgtca tgatcagccg gctgcagagc gccgccgcga gcgacatgcc
     1441 gccgaagcag ccgaccagtc ccgccaccac cgcgacgacc ggcacgtggc ggcgcagcgc
     1501 aacgatggcc gcatgcacct ctgcgatcgc ggcaagcccg aggttggcct cctgcagccg
     1561 caccccgcca gtctcgagca gcagcaccgg ccggaccggc cggccacgct cgcagtcgcg
     1621 cagagcgagc tcgagcgccc cggcgagctt ggcgcccgag acctcgccga cgctgccgcc
     1681 ctgataggcg ccttcgatcg aggcgacgac cgcggacttc ccctcgatcg agccgcgcgc
     1741 gatcacgact ccgtcatcgg cctgcgccac gatgccctgc agtggcagcc acggtgactt
     1801 caggcggtcg aagggaccgg cgagctcgcg gaaggtgccc ggatcgagga tcgcccgcgc
     1861 ccgctcgcgc gcgttgagct cgatgaagct cgc
//
LOCUS       DATLKL010000246         1503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_70619, whole genome shotgun sequence.
ACCESSION   DATLKL010000246 DATLKL010000000
VERSION     DATLKL010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70619"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VM713_03215"
     CDS             <1..1329
                     /locus_tag="VM713_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVH83306.1"
                     /translation="YLTARGSQAWGSVSGDVLTTAGIPACLPSAIAGCFDPAAPQVPD
                     GHQNRSGSLSAGVRLTDQLTASADVLYTHGWTAFDGAGFDDRLDFEEKVFTAHLDQAL
                     GSSWHVRLMAGRDEDDERDFLDPTPVGTFDTVRKSASLQADGKLAEALKLISGVDYED
                     VHVDSDTPFALTSRTTRAVFSELHGDWAGWSALAGARFEHNGQFGNHWTENLGLTRTL
                     EPGLRLVLTWGSAFRAPTFDELYFPGFGNPNLKPETSQSYEAGLDAEEGALRWSLHAY
                     QTTIDEEISIDPLTFLPVNINRSRIRGVELQGDWHDADWVVGGQLTGLEPLNVSPGPE
                     YDDLLPRRAQQSASLSVRRLLRSAPPGSADGSVAVVARWEGRRYDDLANTLPMGGYLT
                     VDLLTQWREGSWTLEARAANLFDRSYQTAAYYAQPGRSYYVTIRYRSSLH"
BASE COUNT          234 a          537 c          507 g          225 t
ORIGIN      
        1 tacctcacgg cgcgcggcag tcaggcctgg ggcagcgtct cgggggacgt gctcaccacc
       61 gccggcatcc ccgcctgcct cccctcggcg atcgccggct gcttcgatcc ggccgccccg
      121 caggtgcccg acggccacca gaaccgctcc ggctcgctga gtgccggcgt gcggctcacc
      181 gatcagctca ccgcatcggc cgacgtgctc tacacccatg gctggacggc gttcgacggc
      241 gccgggttcg acgaccggct ggatttcgag gagaaggtgt tcaccgcaca cctcgaccag
      301 gcgctcggca gtagctggca cgtgcggctc atggccggac gcgacgaaga cgacgagcgc
      361 gattttctcg atcccacgcc ggtgggcacg ttcgacacgg tgcgcaagag cgcgagtctc
      421 caggccgacg gcaagctcgc cgaggcgctc aagctgatca gcggcgtcga ctacgaggac
      481 gtccacgtcg acagcgatac gccgtttgcg ttgacctcgc ggaccacccg cgcggtattc
      541 agcgagctgc acggcgactg ggccgggtgg tcagcgctcg ccggcgcgcg cttcgagcac
      601 aacgggcagt tcggcaatca ctggaccgag aacctcgggc tgacgcgcac actcgagccg
      661 ggcctgcgcc tggtgctgac ctggggctcg gcattccgcg cgccgacctt cgacgagctc
      721 tattttccgg gcttcggcaa cccgaacctg aagcccgaga cctcgcagtc ttacgaggcc
      781 gggctcgacg ccgaggaggg cgcgctgcgc tggtcgctgc atgcctacca gacgaccatc
      841 gatgaggaga tctcgatcga tccgctgacg ttcctcccgg tcaacatcaa caggtcgcgc
      901 atccgtggcg tcgagctcca gggcgattgg cacgacgccg actgggtcgt gggcggacaa
      961 ctcaccgggc tcgagccgct caatgtctct cccgggccgg agtacgacga tctgctgccg
     1021 cgccgcgcgc agcagagcgc cagcctcagc gtgcgtcgcc tgttgcgcag cgctcctccg
     1081 ggcagcgccg acggctcggt cgccgtggtc gcccgctggg aaggccggcg ctacgacgac
     1141 ctcgccaaca cgctgccgat gggcggttac ctgacggtcg atctgctcac gcagtggcgg
     1201 gaaggcagct ggacgctgga agcgcgtgcg gccaacctct tcgaccgcag ctaccagacc
     1261 gctgcatact atgcgcagcc aggccggagc tactacgtca cgatccgcta ccgctccagc
     1321 ctgcactagg gagggtcgac atgagcgagc ccggcgcagg tccggcgccg ggcgcggcgc
     1381 cgaccgagaa cgagcgcacc tggggaatgt tggcgcacct cgcggcgttc gcggggctcg
     1441 tcatgccgct catcggcaac gtggtggtgc cgctcctcat ctggcacagc tggcgcgacc
     1501 gct
//
LOCUS       DATLKL010000247         1605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_126922, whole genome shotgun sequence.
ACCESSION   DATLKL010000247 DATLKL010000000
VERSION     DATLKL010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126922"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /gene="gspM"
                     /locus_tag="VM713_03220"
     CDS             complement(<1..512)
                     /gene="gspM"
                     /locus_tag="VM713_03220"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HVH83307.1"
                     /translation="MTLPEGRSGKILAVSLLALLLALAYLAVLHPLQQFYAARADEIA
                     EKRDRLERLERVAAELPGFRAMLTQLRSSTKRTDFFLEGPSDAVAAASLQSKLKDMIG
                     QVGGEMSSAESLPPNPRGEFRRVGVRVVMVGDLEMLVAVLNSTQQAHPPLFVDDFEIR
                     NRNNFVVAAKT"
     gene            complement(509..1558)
                     /locus_tag="VM713_03225"
     CDS             complement(509..1558)
                     /locus_tag="VM713_03225"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HVH83308.1"
                     /translation="MLGDLFRWWTEQLRGLAPEPLRRLVATGSDAVVVDFGPDFVAFS
                     LRRRGGLEPIGRFARDAQGLKPMRQALARLRARNLRVALRLAPGMVLRKQIALPLAAR
                     RNLPQVLAFEMGRETPFEDGEVFWDNAILQQDRATQRMEVEVAFVPRAQVAEVIDILR
                     AAGGEVSTLEVEAPGRTYRMAMRKDAELDADARWWPATVLGAVAGMLALIAVALPFFR
                     QQQQLAALKDALQAVETQTKAVVGMKKEIEQLSKAANFLAEERARVADPLAILAAATR
                     AIPDDGYLIELVLRDNRLSLVGVSPSAADLIGRLAATKPFQDPVFAAPVVRRDAGKLE
                     LFTISASIGAGGSGS"
BASE COUNT          281 a          548 c          526 g          250 t
ORIGIN      
        1 gtcttggccg ccaccacaaa gttgttgcga ttgcggatct cgaaatcgtc gacaaagagg
       61 ggcggatgcg cctgttgggt cgaattgaga accgctacca gcatctcgag atcgccgacc
      121 ataaccaccc gcaccccgac gcgccggaac tcgccgcgcg ggttgggcgg caggctttcg
      181 gcgctgctca tttccccgcc gacctggccg atcatgtctt tgagcttgct ttgcagactg
      241 gccgccgcaa ccgcatcgct cggtccctcg aggaagaaat cggtgcgctt cgtgctgctg
      301 cgcaactgtg tcagcatggc gcgaaagccc ggcagctccg cggccacgcg ctccaagcgc
      361 tccagccggt cgcgcttttc cgcgatctca tccgcgcgag ccgcgtagaa ttgttgcaac
      421 gggtgcagga cggcgaggta ggcgagcgca agcaacagcg ccagcaggct taccgccaaa
      481 atcttaccgg acctgccctc gggtaaggtc atgaaccgct cccgccggcg ccaatcgagg
      541 cgctgatcgt gaagagctcg agcttgccag cgtcacggcg taccaccggg gccgcgaaaa
      601 cggggtcctg gaacggcttc gtcgcagcga gccgcccgat caggtccgcc gcggagggcg
      661 agaccccaac caggctaagc cggttatcgc gcaagacgag ctcgatcagg tagccgtcgt
      721 cggggatggc gcgggtggcg gcggcgagga tagcgagcgg atcggcgacc cgcgcgcgct
      781 cctcggcgag gaaattcgcc gccttcgaca gctgttcgat ctccttcttc atccccacga
      841 ccgctttggt ctgggtctcc acggcctgca aggcatcctt gagcgccgcg agctgctgct
      901 gctggcggaa gaagggcagc gcgaccgcga tgagggccaa catgccggcg accgccccaa
      961 gcacggtggc gggccaccat cgcgcgtccg cgtccagctc tgcatccttc ctcatcgcca
     1021 tgcggtaggt ccggcccggt gcttcgacct cgagcgtcga aacctctccg ccggccgcac
     1081 gcaggatgtc gatcacctcc gcgacctggg cgcgcggcac gaatgccacc tcgacttcca
     1141 tacgttgggt cgcccggtcc tgctgcagaa tcgcgttgtc ccaaaagacc tcgccatcct
     1201 cgaagggggt ctcgcggccc atctcgaagg cgaggacctg ggggagattg cggcgggcgg
     1261 cgagtggcag ggcgatttgc ttgcgcagga ccattcccgg cgccaggcgc aaggcgactc
     1321 gaaggttgcg cgcgcgcagc ctagcgagcg cttgccgcat gggcttgagg ccctgggcgt
     1381 cgcgcgcgaa tcggccgatg ggctcgaggc cgccgcgtcg gcgcagcgag aacgcaacga
     1441 aatccgggcc aaaatcgacg acgacggcgt cagatcccgt ggcgacgagc cgccgaagcg
     1501 gctccggcgc cagtccccgc aattgctcgg tccaccagcg gaagaggtcc cccagcatgc
     1561 aactactctt cggccaacgt gaaacacaca gtctagcggt cggca
//
LOCUS       DATLKL010000248         1559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_149669, whole genome shotgun sequence.
ACCESSION   DATLKL010000248 DATLKL010000000
VERSION     DATLKL010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149669"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1404)
                     /locus_tag="VM713_03230"
     CDS             complement(67..1404)
                     /locus_tag="VM713_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HVH83309.1"
                     /translation="MKNIAIPFMLLCAAALRAQGVPEAVASQSAALLEIYKDIHAHPE
                     LSHHEARTSAILAKGLREAGYSVTERVGVYPDGSRAFGVVGILKNGAGQTLLIRGDMD
                     ALPIVEETGLPYASHVVTKNASGEEVGVMHACGHDVHTTVLLGTARALAATRAQWHGT
                     VMIIGQPSEETIDGAKAMLADHLYERFGRPDRIIGLHDTNLLAAGTVGIIPGAASASS
                     TSVDVVIKGIGGHGSAPQYGRDPVTLAAYYITQIQTIVSREEDPQDPTVVTVGSIHGG
                     TKRNIIPDEVKLELTTRSYSDKSRQVVIDGLRQIAAGVTTAANLPAEKAATVTVLEHE
                     STPVQYNDPAMTAQVRALLVKALGEARVRAAEKLMGSEDVGVFGMSEDLQRYEIPVVY
                     FRLGAMDPEKLAAAETAGRHLPGPHTSRFEPLPKPTLETGVTAMTAVATGLLQ"
BASE COUNT          222 a          513 c          548 g          276 t
ORIGIN      
        1 cccggtgccg ccgcccgcct gagagggggc agcatgcgac cgcggtaggc gcgccgcgcg
       61 cggtccctac tgcaggagtc cggtcgccac cgcggtcatc gccgtaactc cggtctcgag
      121 cgtcggcttc ggcaacggct cgaagcggct ggtgtgcggg cccggcagat gcctgccggc
      181 ggtttccgcc gccgcgagct tctcgggatc catcgcgccc agccggaagt acaccaccgg
      241 aatctcatag cgctgcagat cctcgctcat gccgaagaca ccgacgtcct cgctccccat
      301 gagcttctcg gctgcccgca cccgcgcctc gcccagagcc ttgacgagga gagcgcgcac
      361 ctgggcggtc atcgccggat cgttgtactg caccggggtc gactcgtgct cgagcaccgt
      421 caccgtggcg gccttttccg caggcagatt cgcggcggtg gtgacgcccg cggcgatctg
      481 cctgagtccg tcgatgacga cctggcggct tttatcggag taggagcgtg tcgtcagttc
      541 gagcttcacc tcgtcaggga tgatgttgcg cttggtgcct ccatggatgc tgccgacggt
      601 gaccacggta ggatcctgcg gatcctcctc gcggctcacg atggtctgga tctgcgtgat
      661 gtagtaggcc gcgagcgtca cgggatcgcg gccgtattgc ggcgcggagc cgtgaccgcc
      721 aatgcccttg atcacgacgt cgacggaagt cgagctcgcc gatgccgctc cgggaatgat
      781 tcccaccgtt ccggcggcca gcaggttggt gtcgtgaagc ccgatgatcc ggtcggggcg
      841 gccgaaacgc tcatagaggt ggtcggcgag catggccttg gcgccgtcga tcgtctcttc
      901 cgacggctgt ccgatgatca tgaccgtgcc gtgccactgc gcgcgcgtcg cggcgagcgc
      961 ccgcgccgtg cccagcagca ccgtcgtgtg cacgtcatgc ccgcaggcat gcatcacgcc
     1021 gacctcctca ccgctcgcgt tcttcgtcac gacgtgactt gcgtagggga ggccggtctc
     1081 ctcgacgatc ggcagagcgt ccatgtcgcc gcggatgagc agcgtctgcc cggcgccgtt
     1141 cttgaggatg ccgacgacgc cgaaggcacg cgacccgtcc gggtacacgc cgacgcgctc
     1201 ggtgacgctg tagcctgctt cgcgcaggcc cttggcgagg atcgcggagg tgcgtgcctc
     1261 gtggtgcgag agctcgggat gcgcatggat gtctttgtag atctcgagga gcgcggcgct
     1321 ctggctcgcg acggcttcgg gcacgccctg cgcacgcagc gcggcagcgc acaggagcat
     1381 gaagggaatg gcaatgtttt tcacggaggc tccccggtcg ggtggcgggc tgttggtggg
     1441 ttcggcgagg gtggcgcggg cgtgacagct tgcccgacaa ccgcacgtgc gctcaaggtc
     1501 ccggttggaa gtgaggcgag tccgtgcgat cttgagcgcg tcccgccaca aggaggaga
//
LOCUS       DATLKL010000249         1727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_81756, whole genome shotgun sequence.
ACCESSION   DATLKL010000249 DATLKL010000000
VERSION     DATLKL010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81756"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /gene="era"
                     /locus_tag="VM713_03235"
     CDS             complement(<1..622)
                     /gene="era"
                     /locus_tag="VM713_03235"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HVH83310.1"
                     /translation="MSAPGPAPFRSGFAALVGRPNVGKSTLLNALVGAKLSIVTPRPQ
                     TTRHRILGIANLPAAQIAFVDTPGLHREGRRALNKAMNRTATAALEDADVVVLVVEAL
                     QWVEGDELALARIARAGRPAILAVNKIDRVRPRERLLPYLARLGGRHDFLAVVPVSAL
                     KADNITDLRDTIAAHLPQSPALFPADTVTDRGLSFRIAETIREKLTL"
     gene            complement(619..1329)
                     /gene="rnc"
                     /locus_tag="VM713_03240"
     CDS             complement(619..1329)
                     /gene="rnc"
                     /locus_tag="VM713_03240"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HVH83311.1"
                     /translation="MTAGGGEQDRWLREALGYEPHDAALFAAALTHRSASGPNNERLE
                     FLGDAVLNLVIAELLYRGFPRAAEGDLSRLRARLVSREPLAEVAAGLRLGEVLRLGSG
                     ELKSGGFRRQSILADALEAVCGALFLDGGLTAAAEVVTRLLAPRIAALPDPATLKDAK
                     TRLQEYLQARSLTLPQYVVERVEGEAHAQTFRVSCEVTSLGRRVLGGGSSRRRAEQEA
                     AARMLLEIGADAPADRQP"
     gene            complement(1355..>1727)
                     /locus_tag="VM713_03245"
     CDS             complement(1355..>1727)
                     /locus_tag="VM713_03245"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HVH83312.1"
                     /translation="MRFRQRGITFIGWLILLTPMVVVGYAAVRLAPVYLNYMKVVRAL
                     NLVVSDAGGNPDPKSIRTTIDKHFEIDMVDYPTAKDIKVTKNGTGWDVEASYDDEAPL
                     FANISLHVVFDKTVHAGGGGP"
BASE COUNT          257 a          606 c          603 g          261 t
ORIGIN      
        1 cgagagtcag cttctcgcga atcgtttccg cgatgcggaa cgacaggccg cgatcggtca
       61 ccgtgtccgc cggaaacagc gccggggatt gcggcaggtg cgccgcgatc gtgtcgcgca
      121 ggtccgtgat gttgtccgcc ttcagcgccg acaccggcac cacggcgaga aagtcgtggc
      181 ggccgccgag cctcgccaga tacggcaaca gccgctcgcg cgggcgtacc cgatcgatct
      241 tgttcaccgc gaggattgcg ggccgcccgg cacgtgcgat ccgcgccagt gccagctcat
      301 cgccctcgac ccattgcagg gcttcgacca ccagcaccac cacatccgcg tcttcgagtg
      361 ccgccgtggc cgtgcggttc atggccttgt tgagcgcgcg ccgtccctcg cggtgcagcc
      421 ccggcgtgtc cacgaaggcg atctgcgccg cgggcaaatt ggcgatgccg aggatgcggt
      481 ggcgggtggt ctgcggccgc ggcgtgacga tgctgagctt cgcgccgacc agcgcgttga
      541 ggagcgtcga tttgccgacg ttgggccgcc cgacgagcgc agcgaagccg ctgcgaaacg
      601 gcgcgggccc gggcgcgctc acggctgccg gtccgcgggc gcgtccgcgc cgatctcgag
      661 cagcatgcgc gcggcggcct cctgctcggc gcgccggcgg ctcgagccgc cgcccagcac
      721 ccggcgtccg agagaggtga cctcgcagct gacgcgaaag gtctgggcgt gagcctcgcc
      781 ttcgacgcgc tccacgacgt attgcggcag ggtgaggctg cgcgcctgca gatactcctg
      841 cagacgcgtc ttggcgtcct tgagcgtagc cggatcgggg agcgcggcga tccgcggcgc
      901 gaggagccgc gtgaccacct cggcggcagc ggtgaggccg ccatcgagaa acagcgcacc
      961 gcacaccgcc tcgagcgcat ccgccaggat cgattggcgg cggaaacctc cgctcttcag
     1021 ctcccccgag ccgagccgca ggacttcgcc gagccgcaag cctgcggcca cctccgcgag
     1081 cggctcccgg ctcacgaggc gggcgcgcag gcggctcaag tccccctcgg cggcgcgcgg
     1141 gaaaccgcga tagagaagct cggcgatgac caggttgagc acggcgtccc cgaggaactc
     1201 cagccgctcg ttgttgggac cggaggcgct gcggtgggtg agcgcggcgg cgaacagtgc
     1261 ggcgtcgtgc ggctcgtagc cgagcgcctc ccgcagccag cgatcctgct cgccgccgcc
     1321 ggcggtcatg tcgcccgtgc gcgctcccgc actgctacgg cccgcccccg ccggcgtgca
     1381 cggtcttgtc gaagaccacg tgcagggaga tgttggcaaa gagcggcgcc tcatcgtcgt
     1441 aggaggcttc cacgtcccac ccggtcccgt tcttagtgac cttgatgtcc ttggcggtgg
     1501 ggtaatccac catgtcgatc tcgaagtgtt tgtcgatcgt ggtgcggatg gacttcggat
     1561 cggggttacc gccggcgtca ctcaccacca ggttgagcgc ccgtaccacc ttcatgtagt
     1621 tgaggtacac gggcgccaga cggacggcgg cgtagccgac caccaccatc ggcgtcagca
     1681 ggatcagcca gccgatgaac gtgatacccc tctgtctgaa gcgcatc
//
LOCUS       DATLKL010000250         1585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_183567, whole genome shotgun sequence.
ACCESSION   DATLKL010000250 DATLKL010000000
VERSION     DATLKL010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183567"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(495..1565)
                     /gene="dnaN"
                     /locus_tag="VM713_03250"
     CDS             complement(495..1565)
                     /gene="dnaN"
                     /locus_tag="VM713_03250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HVH83313.1"
                     /translation="MLTPLQSVIGVVERRQTMPVLENVLLAARDGRLSVTGTDLEVEL
                     VAASQANIQQSGDITVPGRKLLDIFRSLPEKTSVALSTEGERVSLRAGRSRFTLSSLP
                     ASEFPLVEEINAQQTLTVAQGEFRRLIDKTHFSMAQQDVRYYLNGLLLETDGKTLRAV
                     ATDGHRLALCETELAGKAKSSQQVIVPRKGVLELQRILGTDGNIELAVGTNHVRAQIG
                     DIRFTSKLIDGRFPEYGRVIPASPPRTVEAERESLKLALQRTAILSNEKYRGIRLTAR
                     PGLLTIQAHNPEQEEAEDQVEVNYKGEEVEIGFNVNYLLDALGAIESDKVEIGLTDSN
                     SSCLIHAPGTTHTKYVVMPMRL"
BASE COUNT          244 a          505 c          529 g          307 t
ORIGIN      
        1 cgccggaaca gccggaggcg cgctcggtgg aggtctaggg gccgcgggtg gtgtcacagg
       61 ctcagtcggg gaaaccacgg gcggcgcgct tcgggcgggc cccggctcag gtctcgacgg
      121 gtcgggattc ctgaagacag acagctcggg cgtcttcggg ctccgtgacc tgagcctctc
      181 gagcgtagcc tcgggcacct ctcaaggatc gctcgtgacc tcggcgggca agagtgtcca
      241 cctcgactcg gggacgcgcc tcctgctcag ctcgggggca gacggctccg gccatcgtga
      301 tcgcgagcag gctgcgggga agccctccgc ctctgagaag acagagcggg accggcgcta
      361 gcacagcgtc tctgctccct ccgcgaaagt cggcgccgcg accgggttga cccggcgcaa
      421 atgactcgcc ggcggcggct cggtggggcg ttatttctgc gccgattgtt tcacctgaga
      481 gccactcgga tgagctacag gcgcatcggc atcacgacgt acttggtatg cgtagtgccg
      541 ggcgcgtgaa tcagacagct gctgttcgag tccgtcagcc cgatctcgac cttgtcgctc
      601 tcgatggcgc cgagcgcatc gaggaggtaa ttcacgttga agccgatctc gacctcttcg
      661 cctttgtagt tcacctcgac ctggtcctcg gcttcttcct gctccgggtt gtgtgcctga
      721 atcgtgagca gccccggccg cgccgtcagg cggatgccgc ggtatttctc gttcgagagg
      781 atcgcagtgc gttggagggc cagcttcagc gattcgcgct ccgcttcgac ggtccgcggg
      841 ggacttgcgg gtatgactcg cccgtactcc gggaaacggc catcgatcag cttcgaagtg
      901 aagcgaatgt cgccgatctg ggcgcgaacg tgatttgttc cgacggctag ctcgatgttt
      961 ccgtcggttc cgaggatccg ctgcagctcg aggacgcctt tccggggcac gatcacctgc
     1021 tgactgctct ttgccttgcc cgcaagctcg gtctcgcaaa gcgcgagccg atgcccatcg
     1081 gtcgcgacgg cgcgcagcgt cttcccgtcg gtctcgagca gcagcccgtt gaggtagtag
     1141 cgcacgtcct gttgcgccat cgagaagtgc gtcttgtcga tcaggcggcg gaattctccc
     1201 tgcgccaccg tgagggtctg ctgcgcgttg atttcctcga ccagcgggaa ctcggaggcg
     1261 ggcagactcg agagcgtgaa gcggctgcgt ccggcgcgca gcgagacgcg ctcgccttcg
     1321 gtgctgagcg ccacgctggt tttctcgggc aaagagcgaa agatatcgag cagcttccgt
     1381 cccggcaccg tgatatcacc gctctgctga atgtttgcct ggctcgcggc aacgagctcc
     1441 acctcgagat ccgtccctgt cacactgagg cgaccatcgc gcgcagcgag cagtacgttt
     1501 tccagtaccg gcatcgtctg ccgccgctcg acgacgccga tgacgctctg caggggagtg
     1561 agaatatctt cgcgcggggc ggtca
//
LOCUS       DATLKL010000251         3991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_217082, whole genome shotgun sequence.
ACCESSION   DATLKL010000251 DATLKL010000000
VERSION     DATLKL010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217082"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="VM713_03255"
     CDS             <1..1457
                     /locus_tag="VM713_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVH83314.1"
                     /translation="PRVALPEHYDAAAPAGPTAAAVGAPPVELAAWWRALQDPELDSL
                     IERAVSGNPDLLIALDRLQAARTFEAAVIGTVLPEAGASAGAGRGTGSDLTRGRAANA
                     LRSGDNAAGLQHLNEIGGFDAVWELDIFGKYRREIEAARYDEQAVRAARNGVLVVVVA
                     DVANAYVELRGLQVRAAVLASSLAVLRESLRIVTIRYERGITNELDVTLATRELATLE
                     AQAAPLAAQARAAEYTIATLLGLYPEDLVKELETAGLVPAVPSLVDVGVPPELLRRRP
                     DVQQAERELAGATARIGIATAELFPQLAVSGAIGFQRQSLKGQPVLGQHIRSLGPAAV
                     WPLLDFGALDAQVEIADLETRARLVHYKQTIQQAVREVDTSYGLYTAEQQSLGKLDTA
                     LIASQRAVTLANERYQRGVTDFLNVVDAERAEYALEEQYAQAQVAVAGEFIALYRSLG
                     GGWENYQKLPPIHRPEPAIIAMFQRTLSRSDPLR"
     gene            complement(1454..1663)
                     /locus_tag="VM713_03260"
     CDS             complement(1454..1663)
                     /locus_tag="VM713_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HVH83315.1"
                     /translation="MKLSTRNALAGTITRLVRGPVSTEVTIRVAKDVEIVSVITTHSA
                     KRLKLKKGQRAHALIKADHVIVGVD"
     gene            complement(1737..3266)
                     /locus_tag="VM713_03265"
     CDS             complement(1737..3266)
                     /locus_tag="VM713_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HVH83316.1"
                     /translation="MRSSGWCALAALISTSLWTHAARAADPASSAGGCENVHEFLATD
                     CPLTWHGITLYGVYDVGVGWVSHGLPENAYNYEGESLVNRNGLHSQFLIAPNNLSQSG
                     LGIRAKVEFMDNWYVVFNASTGINPQSGQLANMAATNTSNAGLPRASYSITGDGARAG
                     QTINDEFYGGIASAPFGTLTFGRERALGTDAMLAYDPAAGAYSFSYIGYNGLMAGGGD
                     TQDTRWDNATKYRITYGPIHFGAMYKFADGSGGCYSAAAAWTAATCTPEASHNTAFGF
                     DFGGEYANLSADVVFQKVNQAISVVNPLLGPQSPTQPYQSTTNSINQNPITGANLIGT
                     ANTEYGIVTDNTAVMVAAKYTWQQLKFYGGYEHIRQDNPSSPLGVGAQAQGGYLLSGV
                     EDNNLDSPKTVQVWWTGVRYAYDSRTDLVLSYYHQEQNDYRRPPTCTPASFRSSCAGT
                     LNEVSLFADHHFTKRFDAYAGVAYSDVSGGLAIAIPHGPGVHYFYNNNFAPTVGGRFT
                     F"
     gene            3384..3800
                     /locus_tag="VM713_03270"
     CDS             3384..3800
                     /locus_tag="VM713_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVH83317.1"
                     /translation="MRGPAVRFRVDFGRAGAIGPGKIALLEHIASGGSLSQAARELGM
                     SYRRAWQLLESVNSAFRQRAVLTAKGGRGGGGARLTPFGRLLIRAYRDFDAELQRRAA
                     RTFRSISDLTRPAPSDSPRRRRRGAPVVRLKDREGS"
BASE COUNT          631 a         1282 c         1386 g          692 t
ORIGIN      
        1 ggccgcgggt cgcgctcccc gagcactacg acgccgcggc gccagcggga cccaccgcag
       61 cggcggttgg cgcgccgccg gtcgagctcg ccgcctggtg gcgcgcgctc caggacccgg
      121 agctcgactc gctcatcgag cgtgccgtct cgggcaatcc cgatcttctg atcgcgctcg
      181 atcgactgca ggcggcccgc accttcgagg ccgccgtgat cggtaccgtg ctgcccgagg
      241 cgggcgcgtc cgcgggcgca ggccgcggca ccggcagcga tctgacccgt ggccgggcgg
      301 ccaacgcgct gcgatcgggg gacaacgccg caggcctgca gcacctcaac gagatcggcg
      361 gcttcgatgc cgtctgggag ctcgatatct tcggcaagta ccggcgcgag atcgaagcgg
      421 cacgctatga cgagcaggcg gtgcgcgcgg cacgcaacgg ggtgctggtg gtggtggtcg
      481 ccgacgtggc gaacgcctac gtggagctgc gcggcctgca ggtgcgcgcc gcggtactcg
      541 cctcctcgct cgcggtgctg cgcgaatcgc tgcgcatcgt gacgatccgc tacgagcgcg
      601 gcatcaccaa cgagctcgac gtgacgctcg cgacgcgcga gctcgcgacg ctcgaggcgc
      661 aggcggcgcc gctcgcagcg caggcgcgcg ccgccgagta caccatcgcg acactgctcg
      721 gtctctaccc ggaagacctg gtcaaggagc tcgagaccgc gggtttggtc ccggcggtgc
      781 cgtcgctcgt cgacgtgggc gtgccacccg agctgctgcg gcgccgaccg gatgtccagc
      841 aggccgagcg cgagctcgcc ggcgcgactg cgcgcatagg cattgcgacg gccgagctct
      901 tcccgcagct cgctgtcagc ggagcgatcg gcttccagcg ccagtcgctc aagggccagc
      961 cagtgctcgg gcagcacatc cggtcgctgg ggccggcggc ggtgtggcca ctcctggatt
     1021 tcggcgcgct cgatgcgcag gtcgagatcg ccgacctcga gacgcgcgcg cggctcgtgc
     1081 actacaagca gacgatccag caggcggtgc gcgaggtcga caccagctac ggcctgtaca
     1141 cggccgagca gcagagcctc ggcaagctcg acacggcact catcgccagt cagcgcgcgg
     1201 tcacgctcgc caacgagcgc taccagcgcg gcgtcaccga tttcctcaac gtggtcgatg
     1261 ccgagcgcgc ggagtacgcg ctcgaggaac agtacgcgca ggctcaggtg gccgtcgccg
     1321 gcgagttcat cgccctgtac cggagtctgg gcggcggctg ggaaaactat cagaagcttc
     1381 cgcccatcca ccggccggag ccggcgatca tcgccatgtt ccagcgcacg ctgagccgca
     1441 gcgatccgct ccgctagtcg acacccacga tgacgtggtc ggccttgatc agggcgtgcg
     1501 cgcgttgccc cttcttcagc ttcagccgtt tcgccgaatg agtcgtgatc accgacacga
     1561 tctcgacgtc tttggcaaca cgaatggtca cttccgtgct gacaggcccg cggacgagcc
     1621 tggtgatggt gccggccagc gcgttgcgag tgcttagctt catctcgcct cctgacaagt
     1681 cctgctacga gattccgatg ataaccggcg gcggagcggc cgagcgcctc tcgtgatcag
     1741 aaggtgaaac gaccaccgac cgtcggcgca aagttgttgt tgtagaaata gtggacgccg
     1801 ggcccgtgag gaatggcgat ggccagaccg ccgctgacgt cggaatacgc tactcccgca
     1861 taagcgtcga agcgcttcgt gaagtgatga tccgcgaaca gcgaaacctc gttgagcgtg
     1921 cccgcgcagg aactgcggaa actggccggc gtgcaagtcg gcggacgacg atagtcattc
     1981 tgctcctgat ggtagtagga cagcaccagg tcggttctgc tgtcgtaggc gtatctcacg
     2041 cctgtccacc agacctgcac ggtctttggc gaatcgagat tgttgtcttc tacgccgctc
     2101 aggagataac ccccctgagc ctgggcaccg acgcccagcg ggctcgacgg attatcctgc
     2161 cggatgtgct cgtagccgcc gtagaacttg agctgctgcc aggtatattt cgcggcgacc
     2221 atgaccgcgg tgttgtcggt cacgatgcca tattcggtgt tggcggtgcc gatcagattg
     2281 gcacccgtga tggggttttg gttgatgctg ttggtggttg actgataggg ctgcgtcgga
     2341 ctctgcgggc cgagcagggg gttgaccacg ctgatcgcct gattgacctt ctggaagacc
     2401 acatcggctg agagattggc gtactcgccg ccgaagtcga atccaaaggc ggtgttgtgc
     2461 gaggcctccg gcgtacaggt ggcggcagtc caggctgcgg cagcggagta gcagccaccc
     2521 gatccgtcgg cgaacttgta cattgcgccg aaatggatcg gaccgtaggt gatgcggtac
     2581 ttggtggcgt tgtcccaacg ggtgtcctgg gtgtcgccgc cgccggccat caggccgttg
     2641 tagccgatgt acgaaaagga ataggcgccc gccgccgggt cgtaggccag catcgcatcg
     2701 gtgccgaggg cgcgctcgcg accgaaggtg agcgtcccga agggcgccga cgctataccc
     2761 ccgtagaatt cgtcgttgat ggtctggccg gcgcgcgcgc cgtcgccggt gatcgagtag
     2821 ctggccctgg gaagaccggc gttgctggtg ttggtggctg ccatgttggc gagctgcccg
     2881 gactgcggat tgatgccggt cgaggcgttg aataccacgt accagttgtc catgaactcg
     2941 actttggccc ggatgcccag ccccgactgc gacaggttgt tcggcgctat gaggaattgg
     3001 gagtgcaggc cattgcggtt gaccagcgat tcgccttcat agttgtaggc attctccggc
     3061 aagccgtggc tcacccagcc cacgccgacg tcatagaccc cgtaaagggt gatgccgtgc
     3121 caggtgagcg ggcagtcggt ggcgaggaac tcgtggacat tttcgcagcc gcccgcggag
     3181 cttgcgggat cggctgcgcg cgccgcatga gtccaaaggg atgttgagat gagcgccgca
     3241 agggcgcacc agccgctgga ccgcacgtca ctctccctgg cgggtctcgt tatattcgag
     3301 tgaacatatc gtgacagacg ccggcctccc gccgcaagct cgcgccgtcg actgcgctag
     3361 gatggcagcg gaggtatgca ctcatgcggg ggccggcggt gcgttttcgg gtggatttcg
     3421 ggcgtgctgg agccatcggt ccgggcaaga tcgcgctgct cgagcacatc gcgagcggcg
     3481 gctccctctc gcaggcggcg cgcgagctcg gcatgagcta ccgccgtgcc tggcagctgc
     3541 tcgagagcgt gaactccgcg tttcgccaac gggcggtgct caccgccaag ggagggcgtg
     3601 ggggcggcgg cgcgcgtctc acgcccttcg gtcggctcct catccgcgcc taccgtgatt
     3661 tcgatgccga gctgcagcgg cgcgctgcgc gcaccttcag gtcgatctcg gatctcacac
     3721 ggccggcgcc atcggacagc ccgcgccgcc gccgacgggg cgccccggtc gtgcggctca
     3781 aggatcgcga gggcagctga ttgggctttg gcggacggcc cagctcagtc ctggcggtgt
     3841 tccgccacta cctcgtcact ggtgggattc gccttcgccc tgccgttgac gaacacgacg
     3901 ggaatcacgt tgctcttgag gacctcggcg gcgcgattga cgttcggagc gttgtccggc
     3961 gtgacgcgtg cgatcgagta gtggttggag c
//
LOCUS       DATLKL010000252         1662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_104439, whole genome shotgun sequence.
ACCESSION   DATLKL010000252 DATLKL010000000
VERSION     DATLKL010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104439"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VM713_03275"
     CDS             complement(<1..477)
                     /locus_tag="VM713_03275"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVH83318.1"
                     /translation="MASDVVDLHEFYGSGLGQMARRLIRRKVRRIWPSAKGQRVLGVG
                     YATPYLRQFRDEAQRVIAVMPAAQGVMRWPDGEPNLVTLADEAELPLPDLSADRVLLV
                     HALEHSEQLRPLLREVWRVLADGGRLLVVAPNRRGIWARLDRTPFGQGLPYTMGQLS"
     gene            597..1367
                     /gene="gloB"
                     /locus_tag="VM713_03280"
     CDS             597..1367
                     /gene="gloB"
                     /locus_tag="VM713_03280"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HVH83319.1"
                     /translation="MSTLEIDLVPCLRDNYAYLLRDSATGATGVVDPSEAPPVLKALE
                     RRGWKLTHILNTHHHWDHTGGNPALKAETGAVVVGPKPDEARIPDIDVALDEGERWKL
                     GEAEARILFIPGHTRGHIAFWFPESKAVFCGDTLFAIGCGRLFEGTPAQMWTSLGKLR
                     ALPPETRVYCGHEYTQANARFALTVEPENAKLRARAAEVDRARARGLPTIPSTIGEER
                     DTNPFLRADQPILARNIGLAGADPVAVLAEVRRRKDHF"
     gene            1390..>1662
                     /locus_tag="VM713_03285"
     CDS             1390..>1662
                     /locus_tag="VM713_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVH83320.1"
                     /translation="MKLRFSATSPYVRKVMATAHETGLESRIERVPAAVWVPDTDIAK
                     HNPLGKVPALITGEGEVLYDSPVICEYLDSLHKGPKLFPAAGTARWK"
BASE COUNT          259 a          615 c          560 g          228 t
ORIGIN      
        1 cgagagctgg cccatggtgt agggcaggcc ctggccgaac ggggtgcggt cgagccgcgc
       61 ccagatgccg cgccgattcg gcgccaccac caggagccgc ccgccgtcgg ccagcacacg
      121 ccacacctcg cgcaacaggg ggcgcagctg ctcgctgtgc tcgagcgcgt ggacgagcag
      181 cacgcggtcg gccgaaaggt cggggagggg cagctccgcc tcgtcggcca gcgtcaccag
      241 gttgggctcc ccgtccggcc agcgcatcac gccctgcgcc gccggcatga cggcgatgac
      301 gcgctgggcc tcgtcgcgga actggcggag gtagggggtg gcgtagccta ccccgagcac
      361 gcgctggcct ttagcgctcg gccagatccg gcgcactttg cggcggatca gccggcgcgc
      421 catctggccg aggccggagc cgtagaactc gtgcagatcg acgacgtcac tcgccatggc
      481 gggcgggagg atagcatagc taccatcggg tcgcgcggcg cggggtggca gggcgccgcg
      541 gctgtgctag gctccgccct tcgccacttg cgcagcctca aggattgccg ggccccatgt
      601 cgaccctcga gatcgatctc gtcccctgcc tccgggacaa ctacgcctat ctcctccgcg
      661 attctgccac cggggcgacc ggagtggtcg acccctccga ggcaccgccg gtgctcaagg
      721 cgctcgagcg gcggggctgg aagctcaccc acatcctcaa cacgcaccac cattgggacc
      781 ataccggcgg caaccccgcg ctcaaggccg agaccggtgc cgtcgtcgtc ggccccaagc
      841 ccgacgaggc gcgcatcccg gacatcgacg tggcgctgga tgagggcgag cgctggaagc
      901 tcggcgaggc cgaggcgcgc atcctgttca tccccggcca tacgcgcggc catatcgcct
      961 tctggtttcc cgagagcaag gcagtcttct gcggcgacac tctgttcgcc atcggctgcg
     1021 ggcggctgtt cgagggcacg cctgcgcaga tgtggacgtc gctcggcaag ctgcgcgcgc
     1081 tcccgcccga gacgcgcgtt tattgcggcc acgaatacac ccaggcgaac gcccgcttcg
     1141 cgctcaccgt cgagcccgag aacgcgaagc ttcgcgcccg cgccgccgag gtcgaccgag
     1201 cgcgcgccag ggggcttccc accattccct cgacgatcgg cgaggagcgc gacaccaacc
     1261 ccttcctgcg cgccgaccag cccatcctgg cccgcaatat cggcctcgct ggcgccgacc
     1321 ccgtcgccgt cttagccgag gtgcggcggc gcaaagacca tttctgaccg cgaaatcgag
     1381 gagcccgcca tgaagctccg cttttccgcc acctcgccct atgtgcgcaa ggtgatggcg
     1441 accgcccacg agacggggct cgaaagccgc atcgagcgcg tgcccgccgc ggtgtgggtg
     1501 ccggacacgg acatcgccaa gcacaacccg ctcggcaagg tgccggcgct catcacgggc
     1561 gagggcgagg tgctctacga ctcgccggtc atctgcgagt atctcgacag cctgcacaag
     1621 gggcccaagc tgttccccgc cgcgggcacc gcccgctgga ag
//
LOCUS       DATLKL010000253         1622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_172082, whole genome shotgun sequence.
ACCESSION   DATLKL010000253 DATLKL010000000
VERSION     DATLKL010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172082"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..>1622)
                     /locus_tag="VM713_03290"
     CDS             complement(97..>1622)
                     /locus_tag="VM713_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83321.1"
                     /translation="FLPGEPAEHSRSAGACRGVFALAAACADGAAAGDAAARDALLPG
                     RKGVHAGAPARSVPPPPDAQRPPGAGGFSGALPQPRRARPKAFVDWQNDVTTKDLALA
                     TREGFRSIEHVKRYTTTGMATDQGKTSNVNALGIVARTLGKSIPEVGLTTFRMPYTPV
                     TFGAFAGVARDELFDPVRTTPMHGWATAQGAVYEDVGLWKRARYFPRAGEDLHAAVAR
                     ECRAARGGCGIFDASTLGKIEVVGSDAVTFMNRMYVNAWDRLAVGRARYGVLLRDDGF
                     VYDDGVVARLAADRFHVTTTTGGAPRVLALMEDYLQTEWSALNVWLTSTTEQWAVIAI
                     QGPAARAVLAGLVDIDISAQAMPHMSMAEGSILGVPMRLFRVSFTGELGFEVNVPADF
                     GAAVWEAVWDAGQPHGMTPYGTEAMHVMRAEKGYIIVGQDTDGTVTPDDAGLAWTIGK
                     AKPDFVGMRSLQRPAMAAPTAGSWSDSARRIRRSCSRKACRCWRNRACGRRRVRWDT"
BASE COUNT          238 a          577 c          575 g          232 t
ORIGIN      
        1 ccggcacata gagcgtctgc cccatgcgcg cgcggccgcc cgcaaccagc ccgagtgcga
       61 tggagcggcc gaggacagcg ctgtggtagg aggatgtcac gtgtcccaac ggacgcgtcg
      121 gcggccgcat gcccggttgc gccagcacct gcacgccttc ctcgagcacg acctgcggat
      181 ccttcgggct gagtccgacc agctgccggc ggtcggcgcc gccatggcgg gacgctgcag
      241 cgagcgcatg ccgacaaagt cgggcttggc cttgccgatc gtccacgcca gccccgcatc
      301 atcgggcgtg accgtcccgt ccgtatcctg tccgacgatg atgtagccct tctcggcgcg
      361 catcacgtgc atggcctcgg tgccgtaggg cgtcatgccg tgcggctgtc ccgcatccca
      421 caccgcttcc cacaccgcgg cgccgaaatc cgccggcacg ttgacctcga agccgagctc
      481 gccggtgaag ctcacacgaa agagccgcat cggcacccca aggatgctcc cctccgccat
      541 gctcatgtgc ggcatggcct gcgccgagat gtcaatgtcg acgagacctg cgagcaccgc
      601 gcgtgccgcg ggaccctgaa tcgcaatcac ggcccactgc tcggtggtcg aggtgagcca
      661 cacgttgagc gcgctccact cggtctgcag gtagtcctcc atgagcgcaa gcacccgcgg
      721 tgccccgccg gtggtggtcg tcacgtggaa gcggtccgcg gcgaggcgcg ccaccacgcc
      781 gtcgtcgtag acgaagccat cgtcgcgcaa gagcacgccg tagcgcgcgc ggccgacggc
      841 aagccggtcc caggcgttga cgtacatgcg gttcatgaac gtcaccgcat cgctccccac
      901 gacctcgatc ttgccgagtg tggaggcgtc gaagatgccg cagccgccgc gcgctgcgcg
      961 gcattcgcgc gccaccgccg cgtgcagatc ctcgccagcg cgcgggaagt agcgggcgcg
     1021 cttccagagc cccacgtcct cgtatacggc gccctgggct gtggcccagc cgtgcatggg
     1081 ggtggtgcgc accggatcga agagctcgtc gcgggccact ccggcgaagg cgccgaaagt
     1141 gaccggggtg tacggcatgc ggaaagtggt gagcccgacc tcggggatgc tcttgccgag
     1201 agtgcgcgcc acgatgccga gagcattgac gttcgaggtt ttgccctggt cggtcgccat
     1261 gccggtcgtg gtgtagcgct tgacgtgctc aatcgagcgg aagccctcgc gcgtcgcgag
     1321 cgccagatcc ttggtggtga cgtcgttctg ccagtcgacg aaggccttag ggcgcgcgcg
     1381 ccggggttgg ggcagggccc cgctgaagcc gcccgctccg gggggccgct gggcatcggg
     1441 gggcggcggg acgctgcgcg cgggagcccc ggcgtgtaca cccttgcgac cgggcagcag
     1501 cgcgtcgcgg gccgccgcat cgcccgccgc ggcgccgtcc gcgcaggccg cagcgagcgc
     1561 aaagaccccg cggcacgcgc cagcgctgcg ggagtgctcg gccggctcgc cggggaggaa
     1621 tg
//
LOCUS       DATLKL010000254         1563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_172087, whole genome shotgun sequence.
ACCESSION   DATLKL010000254 DATLKL010000000
VERSION     DATLKL010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172087"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..>1563)
                     /locus_tag="VM713_03295"
     CDS             complement(6..>1563)
                     /locus_tag="VM713_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVH83322.1"
                     /translation="GIDVKVPGMLFAAVRCAPRFGSALEHYDGASIRRQPGVIALVEV
                     PQGLAVVARSYWQAHEALYGAELRWSDTGSTLATGDGLPAVYQERLKSGSFDALKTAG
                     DPEKVLAGSARRLKAVYELPFQAHATMEPMNCTAHVTAERCEVWGPIQGVELAHNVAM
                     QVTGLASERVTIHRTLLGGGFGRRLLGDFVKQAVIVSKSVNAPVKLIWSREEDMTRDA
                     YRAAMLHEVSGALDASGRLLVLRHRVVSPSHMLYIWPRGMFPQLKDWSEPVAPPPQYD
                     TMAVEGLIEVPYEVPHQLVSQHRLELDVPVSVWRTTGHGPNNFVLESFIDELAHDAEV
                     DPVALRRRALGKNPRALKVLDTLTEKAGWGRSPAAGTGRGLALAGAFGGLVGCAVELS
                     VSASEIHIHRVVEVVDCGRTLDPGIASSNIAGGIVWGLSAMKTAMRFENGRAAYFNFD
                     GFEPLHLWETPPSEIHFLESGAQLGGTGELGPVPVHAAVGNALFALTGQRIRALPLSR
                     SGLTLHAARS"
BASE COUNT          258 a          542 c          523 g          240 t
ORIGIN      
        1 cctgatcagc tgcgcgctgc atggagcgtc aaccccgagc gcgagagggg cagcgcgcgg
       61 atgcgctgtc cggtgagcgc gaacagtgca ttcccaacgg cggcatgtac cggcaccggt
      121 ccaagctcgc ccgtgccgcc gagctgggcg ccgctttcga gaaaatggat ctcgctcggg
      181 ggcgtttccc acaggtgcag gggctcgaaa ccgtcgaagt tgaaatacgc ggcgcgcccg
      241 ttctcgaagc gcatcgccgt cttcattgcc gaaagccccc agacgatacc gccggcgatg
      301 ttcgaggagg cgatgccggg atcgagcgta cggccgcaat cgaccacctc caccacgcga
      361 tggatgtgga tctcggaggc ggagacgctc agctccacgg cgcagccgac gagtccgccg
      421 aacgcacccg ccagcgccag tccgcggccg gtgccggccg cgggcgagcg gccccacccc
      481 gccttctccg tcagcgtatc gaggaccttg agggcgcgcg ggttcttgcc gagggcgcgg
      541 cgccggaggg ccaccggatc gacttcggcg tcgtgggcga gctcatcgat gaagctctcg
      601 agcacgaagt tgttcgggcc atggcctgtc gtgcgccaca ccgacacagg gacgtcgagc
      661 tcgagccgat gctgactgac gagttgatga ggcacctcgt aggggacctc gattagtccc
      721 tcgacggcca tcgtgtcgta ctgcggcggc ggcgcgactg gctcgctcca gtccttgagc
      781 tgcggaaaca tgccgcgtgg ccagatgtag agcatgtgcg agggcgagac gacccgatgg
      841 cgcagcacga gcagccgccc cgacgcatcg agcgcgccac tcacctcgtg cagcatcgcg
      901 gcacggtagg catcgcgagt catgtcctcc tcgcgcgacc agatgagctt cacgggcgcg
      961 ttgaccgact tcgagacgat cacggcctgt ttgacgaagt ccccgagcaa ccggcggccg
     1021 aagccgccgc cgaggagcgt ccggtggatc gtgacgcgct cgctcgcgag cccggtcacc
     1081 tgcatcgcga cgttgtgtgc cagctcgacc ccctggatcg gaccccacac ctcgcagcgc
     1141 tcggccgtca cgtgcgcggt gcagttcatc ggctccatcg tggcgtgcgc ttgaaacggc
     1201 agctcgtaga ccgccttaag ccgacgtgcg ctgccggcca gcaccttctc aggatcgcct
     1261 gcggtcttca gtgcatcgaa ggagccggac ttgagccgct cttggtagac cgccggtaga
     1321 ccatcgcccg tggcgagcgt gctgccagtg tcgctccagc ggagctcggc gccatagagt
     1381 gcctcgtgcg cctgccagta gcttcgggcc acgacggcca acccctgcgg cacctcgacg
     1441 agggcgatga cgcccggctg tctgcgaatg ctcgcgccat cgtaatgctc gagcgcgcta
     1501 ccgaagcgcg gcgcgcagcg caccgccgcg aatagcatgc ccggcacctt gacgtcgatc
     1561 ccg
//
LOCUS       DATLKL010000255         2995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_217137, whole genome shotgun sequence.
ACCESSION   DATLKL010000255 DATLKL010000000
VERSION     DATLKL010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217137"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VM713_03300"
     CDS             <1..920
                     /locus_tag="VM713_03300"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVH83323.1"
                     /translation="IGHASLDNYVAMISGQAPNHETQLDCPTYSDFRLTSPSLNADGQ
                     ALGAGCVYPAIVRTLPDQLEASGLTWKGYMEDMGNDPKREPRTCGHVPLGAADPTSVA
                     RRGDQYAAKHDPFVYFHTIIDDAARCNAHVVNLDRLPQDLASAAATPNFVFITPNLCS
                     DGHDPKCVDGREGGLAAINIFLAQWVPLIVNSAAFRADGLLIVTFDEADGIGKEGSGA
                     CCGEKPLPGARYAPGFNGPGGGRVGAVILSPFVRPGSVSAVPYNHYSLLKTVEAIFGL
                     QPLGYAASSGAEVFGAEVFSAAALHPPAR"
     gene            complement(886..1362)
                     /locus_tag="VM713_03305"
     CDS             complement(886..1362)
                     /locus_tag="VM713_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83324.1"
                     /translation="MLTGRRISSRNTFFVKRVLPPLLCGVLALGVVLPFALTQGGPHA
                     PPWPVFVAPTAVMVIIYAVLKKLVLDLADEVVDEGEALRVRFGKEEERIPLAQIINVS
                     YAGHTNPPRITLTLRSAGRFGREVTFSPQQGFLSPLFRPNPLVDELIERVDAARRR"
     gene            1485..1979
                     /locus_tag="VM713_03310"
     CDS             1485..1979
                     /locus_tag="VM713_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVH83325.1"
                     /translation="MKAELPPAAQLPEGGLSVGYLMARARASLLAGLDAEFAPFGLNA
                     MQYAVLKHLADGTARTAADLCRHMYYDTGSMTRILDHLESKGLLRRERCQDDRRVVFL
                     RVAAAGRAQLPRLRAISSRVLEEHLAGFKTAEVENLKSYLGRMIENGKLLGGAPHHAR
                     TGKA"
     gene            1976..>2995
                     /locus_tag="VM713_03315"
     CDS             1976..>2995
                     /locus_tag="VM713_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVH83326.1"
                     /translation="MITHRSLTILPLLGAAALSGCVDRGGWKATPEIAPAQLTAARTL
                     AATPVDAAAWPADGWWHGYGDPQLDALVAEAIAGSPSLTAAEARLRAAQGQALAVGAL
                     KLPNVALDAEVTRQRYPENGLYPPPFAGHYYSDGRVAFDLSYDIDFWGRNRALLAAAR
                     SGVDAAEADRAAARLALAVAVVRAYVQLDLWYSLLDVANDQLKQQTSIMELTQQRVEA
                     GLENTARVKQSEASLAFARAGIAAAQANIDLARNQLAALVAAGPDRGQALARPHLAAP
                     ASIALPAALPADLLGRRPDVAAARAQVQGAAQGVAAAEADFYPNVNLAAFGGLQSIGL
                     DKLFEG"
BASE COUNT          485 a         1054 c         1016 g          440 t
ORIGIN      
        1 cgatcgggca tgcgagccta gataattacg tggcgatgat cagcggccag gcaccgaacc
       61 acgagaccca gctcgactgc ccgacctaca gcgatttccg cctcacttca ccctcgctca
      121 acgccgacgg ccaggcactc ggcgcgggct gcgtgtaccc ggcgatcgtc aggacgctgc
      181 cggatcagct cgaggcgtct ggcctcacct ggaagggcta catggaggac atgggcaacg
      241 atccgaagcg cgagccccgc acgtgcggac acgtgcccct cggagctgcc gatccaacca
      301 gcgtcgcgcg gcgcggcgac cagtacgccg ccaaacacga cccgttcgtg tacttccata
      361 cgatcatcga tgacgcggcg cgctgcaatg cgcacgtcgt gaatctcgat cgcttaccgc
      421 aggacctggc gagcgccgcc gccactccca acttcgtctt catcacgccg aacctctgca
      481 gcgacggcca cgatcccaag tgtgtcgacg gacgcgaggg cgggctcgcg gcgatcaaca
      541 tcttcctcgc ccaatgggtg ccgcttattg tcaactccgc ggcgttccgc gccgacggcc
      601 tgctcatcgt cacgttcgac gaggcggacg ggatcggcaa ggagggctcg ggagcctgct
      661 gcggcgagaa gccgctgccg ggagcgcgct acgcgccggg cttcaacggg ccaggcggcg
      721 gacgcgtggg cgcggtgatc ctctcgccgt tcgtgaggcc tggctcggta tcggcggtgc
      781 cgtacaacca ttactcgctg ctgaagaccg tcgaggcgat cttcgggctg cagccgctcg
      841 gctacgccgc ctcatccggg gcagaggtgt tcggggcgga ggtgttcagc gccgccgcgc
      901 tgcatccacc cgctcgatga gctcgtcgac gagcggattc gggcggaaga gggggctcag
      961 gaaaccctgc tgcggcgaga aggtcacctc gcgaccgaag cgtcctgcgc tgcgcaacgt
     1021 cagcgtgatg cgtggcggat tggtgtgacc cgcatagctc acgttgatga tctgcgcgag
     1081 cgggatacgc tcctcctcct tgccgaagcg cacccgcagc gcctcgccct cgtcgaccac
     1141 ctcgtcggcc agatcgagca ccagtttctt cagcacggcg tagatgatca ccatcaccgc
     1201 cgtaggcgcc acgaataccg gccacggagg cgcatgggga ccgccttgcg tgagcgcaaa
     1261 cggcagcacc actccgaggg ccagcacgcc gcagagcagc ggcggcagaa cccgcttcac
     1321 gaagaaggtg ttacgcgagg aaatgcgtct cccggtgagc accggctcct ccctctttag
     1381 tgcagtcgac aaatacctgc caaggcagta gtcttactgg cagcaattct gagcgtggcg
     1441 gcccgcgcgc cgccagcggg gcgcacgtga gtcgcagggc caccatgaaa gccgagctgc
     1501 cgccggcagc gcaactcccg gaggggggat tgagcgtcgg ctacctgatg gcgcgcgccc
     1561 gcgcctcgct cctcgccggg ctcgatgccg agttcgcccc gttcgggctg aacgcgatgc
     1621 aatacgcggt gctcaagcac ctcgcagacg gcactgcccg caccgcggcg gacctctgcc
     1681 ggcacatgta ctacgacacc ggatccatga cacgcattct cgaccacctc gaatcaaagg
     1741 gcctgctgcg gcgcgagcgc tgccaggacg atcgccgggt agtgtttctg cgcgttgccg
     1801 cggccggacg cgcccagctg ccgcgcctgc gcgccatcag cagccgggtg ctggaggaac
     1861 acctggcagg gttcaagacg gccgaggtgg agaatctgaa gagttacctc ggacgcatga
     1921 tcgaaaacgg aaaactcctg gggggtgctc cgcaccacgc tcgaacaggc aaagcatgat
     1981 cactcacagg tctctcacga ttctgccgct gctcggcgcc gcggcgctct ccggctgcgt
     2041 cgaccgcggg ggctggaagg ccacccctga gatcgccccg gcgcagctca ccgcggcacg
     2101 caccctcgcc gccacgccgg tcgatgccgc ggcctggccg gcggatggct ggtggcatgg
     2161 ctacggcgat ccgcagctcg atgcgctggt cgccgaggcg atcgccggga gcccctcgct
     2221 cactgccgcc gaggcgcgcc tgcgcgccgc gcagggacag gcgctcgcgg tcggcgcgct
     2281 gaagctcccc aacgtggcgc tcgatgcgga ggtgacccgg caacgctacc cggagaacgg
     2341 cctgtacccg ccgccctttg cgggccacta ctacagcgac gggcgtgtcg cgttcgactt
     2401 gagctatgac atcgacttct ggggccgcaa tcgcgcgctg ctcgccgccg cccgctccgg
     2461 ggtggatgcg gccgaggccg atcgtgccgc ggcgcggctc gcgctcgccg tagcggtggt
     2521 gcgtgcctac gtgcagctcg atctatggta ttcgctgctc gacgtcgcga acgatcagct
     2581 caagcagcag acctccatca tggagctcac gcagcagcgc gtcgaggcag ggctcgagaa
     2641 caccgcgcgc gtcaagcagt ccgaggccag cctggcgttc gcccgcgccg gcatcgccgc
     2701 ggcgcaggcc aacatcgatc tggcgcgcaa tcagctcgcg gcgctggtcg ccgcggggcc
     2761 ggatcgcggc caggcgctcg cgcggccgca cctcgcggcg ccggcgagca tcgcgctgcc
     2821 cgccgcgctg cccgcggacc tcctcgggcg ccggccggat gtggcggcgg cgcgggccca
     2881 ggtgcaaggg gcggcgcagg gggtcgcagc ggcggaggcc gacttctatc ccaacgtgaa
     2941 cctggcggcg ttcggggggc tgcagagcat cggtctcgac aagctgttcg agggc
//
LOCUS       DATLKL010000256         2231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_93212, whole genome shotgun sequence.
ACCESSION   DATLKL010000256 DATLKL010000000
VERSION     DATLKL010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93212"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VM713_03320"
     CDS             <1..958
                     /locus_tag="VM713_03320"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HVH83327.1"
                     /translation="VSAWYLLTGRHQALAKRSLTVAASFGLASALSVVVLGDESGYTA
                     TINQKMKIAAIEAMGETETPPASFTLYGIPDQARHITRDPIRIPWLLGLIATRSLDEP
                     VQGINQLVAAAEQRIRNGLIGYRLLADMRQHPEAAISPSTQPFLDDLGYALLLKRYAA
                     DIASASDAQIHQAALDTVPDVLVLFWAFRVMVACGLYFILLFALAFYLASRRRIEQGW
                     FLGLALVSLPLPWIAAELGWIVAEYGRQPWVVESVLPTFLGASTLPAGNVWLSIAGFV
                     LFYSTLAIVDVYLMVKYIRIGPEPSPASDSRAAPALGEGLRR"
     gene            955..2103
                     /gene="cydB"
                     /locus_tag="VM713_03325"
     CDS             955..2103
                     /gene="cydB"
                     /locus_tag="VM713_03325"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HVH83328.1"
                     /translation="MIVPLDYGLLRFIWWALLGALFIGFAVMDGFDLGVAMLQPMVAR
                     SDSEKRVLLNAVGPVWEGNQVWFVLGGAASFAAWPPLYAVSFSGFYLAMFLVLAALIL
                     RPVAFTFRSKLESVAWREIWDWAFFIAGLVPALIFGVAFGNLLRGVPFQFDDELRVTY
                     EGGLLGLLNPFAILSGLISVAMLAMHGGTYLALKTEGELAARATRAVGRAALALIVLF
                     TLAGLWVAFGMEGYAIEGTINPAGPSNPLGKTVVRAAGVWLTNYGIHPWLIAAPALGY
                     LGALAAAVLVRVRLAGIAFVASSASVAGVIATAGVSLFPFLLPSSSDPKSSLTVWDSS
                     SSRPTLMVMLAAMVVFLPLIVAYTSWVFSVLRGKITIEEIEQDKKGLY"
     gene            2127..2228
                     /gene="cydX"
                     /locus_tag="VM713_03330"
     CDS             2127..2228
                     /gene="cydX"
                     /locus_tag="VM713_03330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HVH83329.1"
                     /translation="MWYFSWILGLGLACSFAILNAMWFELDEPHERD"
BASE COUNT          314 a          753 c          721 g          443 t
ORIGIN      
        1 ggtgagcgcc tggtatctgc tcacgggccg ccaccaggcg ctggccaagc gctcgctgac
       61 ggtcgccgca agcttcgggc tggcctcggc cctttcggtc gtcgtgctgg gggatgagag
      121 cggctacacc gcgacgatca accagaagat gaagatcgca gcgatcgagg cgatggggga
      181 gaccgagacg ccgcccgcca gcttcacgct ctatgggatc ccggaccagg cgcgccatat
      241 cacgcgcgac ccaatccgga tcccgtggct gctgggactg atcgcgactc gctcactcga
      301 cgagcccgtc cagggtatca atcagctggt cgccgcggcc gagcaacgca tccgcaacgg
      361 gctcatcggc tatcgtctgc tcgccgatat gcgccagcac cccgaggcgg cgatctcgcc
      421 ctcgacgcag ccgttcctcg acgatctcgg ttacgcgctg ctcctgaagc gatacgcggc
      481 cgacatcgcg agcgcgagcg acgcccagat ccaccaggcc gcgctcgaca cggttcccga
      541 cgttctcgta cttttctggg cgttccgcgt gatggtcgcc tgcggcttgt atttcatcct
      601 cctcttcgcg ctcgccttct acctcgcctc acgccgcagg atcgagcagg gctggttcct
      661 cggactcgcg ctcgtgagcc tgccgctccc gtggatcgcg gcggagctcg gctggatcgt
      721 cgcggagtat ggccgccagc cctgggtggt cgaaagcgtg cttccgacct tccttggcgc
      781 ctccacgctg ccggccggca acgtctggct cagcatcgct ggcttcgtgc tgttctactc
      841 cacgcttgcg atcgtcgacg tctatctcat ggtcaaatac atccgcatcg ggcccgagcc
      901 ctcgccagcc agcgacagcc gcgccgcgcc ggcgctcggg gagggactga ggcgatgatc
      961 gttccgcttg attacgggct cctccgcttc atctggtggg ctctcctcgg cgcgctcttc
     1021 atcggctttg ccgtgatgga cggctttgac ctcggggtcg cgatgttgca gccgatggtg
     1081 gcgcggagtg actccgagaa gcgggtgctg ctcaacgcgg tcggcccggt gtgggagggc
     1141 aaccaggtct ggttcgtgct cggcggcgcc gcctcgttcg cggcatggcc gccgctctac
     1201 gccgtgtcct tctcgggctt ctatctcgcc atgttcctgg tgctcgccgc gctaatcttg
     1261 cggcccgtcg ccttcacctt tcgcagcaag ctcgagagcg tggcgtggcg cgagatctgg
     1321 gattgggcct tcttcatcgc cggcctggtg cccgcgctga tcttcggcgt ggcgttcgga
     1381 aacctgctgc gtggcgtgcc cttccaattc gacgatgagc tcagggtcac ctatgagggc
     1441 gggctgctgg ggctgctcaa cccgttcgcc atactgagcg gactgatcag cgtcgcgatg
     1501 ctggcaatgc acggggggac ctatcttgcg ctcaagaccg aaggtgagct cgccgcgcgc
     1561 gccacgcgcg cggtcggacg agcggcgctg gcgctcatcg tcttgttcac gctggccggc
     1621 ttgtgggttg cgtttgggat ggagggatac gccatcgagg ggaccatcaa tccggccgga
     1681 ccgtccaacc cactcggcaa gacggtcgtc cgagccgccg gcgtttggct gacgaactac
     1741 ggcatccacc cctggctcat cgccgcgccc gcgctcggct atctgggggc gctcgccgcc
     1801 gcagtactcg tccgcgtgcg attggcgggg atcgcattcg tcgccagcag cgccagcgtg
     1861 gccggcgtga tcgccaccgc cggggtcagc ctgttcccct tcctcctgcc ctcctcgagc
     1921 gatcccaaga gtagcctcac cgtgtgggac tcttcgagca gccggccgac cctcatggtc
     1981 atgctcgctg ccatggtggt attcctgcca ctcatcgtcg cgtacacgag ttgggtgttc
     2041 agcgtgctgc gcggcaagat caccatcgag gaaatcgaac aggacaagaa gggcctctac
     2101 tagactttgg cgagaggtgc caagcgatgt ggtatttttc ctggatcctg ggcctcgggc
     2161 tggcctgctc cttcgcgatc ctcaatgcta tgtggtttga gctcgacgag ccccacgagc
     2221 gggactgaag g
//
LOCUS       DATLKL010000257         2881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_70767, whole genome shotgun sequence.
ACCESSION   DATLKL010000257 DATLKL010000000
VERSION     DATLKL010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70767"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /gene="rlmKL"
                     /locus_tag="VM713_03335"
     CDS             <1..1143
                     /gene="rlmKL"
                     /locus_tag="VM713_03335"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HVH83330.1"
                     /translation="SAAVLTGAPELGLELGLRAYRTHILWNGAIECRLLRIEVGPESE
                     RAPGTLGRGVAHLKDTAGARMFANRLTKNLKRLRGWAERSGVSCYRLYEADMPEYAFA
                     IDLYRTVDTQADWLYVQEYSAPAEIEREAVQRRRGEVLASLPAVAGVPPERIRVRTRR
                     RTRRGEQYEKLGERQRFHVVAEGGLKFRVNFDDYLDTGLFLDHRLTRARLRSAAPAKS
                     FLNLFAYTGTATVYAAAGGAHASVSVDLSRTYLEWMQQNLALNGLAGAAHALVQADCR
                     EWLAGAGARAPRFDLIFLDPPTFSNSKRMAGVLDIARDHRQLIEGCVRLLAPAGLLVF
                     STNAQRFKLAPELTERFSIVDVSAATLPEDFARNPRIHRCYEFRVR"
     gene            1150..2172
                     /locus_tag="VM713_03340"
     CDS             1150..2172
                     /locus_tag="VM713_03340"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HVH83331.1"
                     /translation="MPSERRSKPAVVAIVGATGAVGVEMIGCLERRRFPLAELRLLAS
                     ARSAGKTLEFRGAPLTVRELTEASFAGVDLALFSAGSAVAKKFAPLAARAGAIVIDNS
                     SAFRMDPEVPLVVPEINQQAARAHRGIIANPNCTAIISATPLWPVNRANRIVRLLIAS
                     YQSASGAGAAAMEELRESTRAYLEGRSFEPRVLPHPYAFNVFSHNARVDPASGYNEEE
                     IKVRDETRKIFGDPELRVSATCVRVPVLRAHSVAINFECQRPITPEEVRRLIADAPGV
                     KLVDDRERNYFPMPRDASGGDPILVGRIRQDVSDPSGRSIALFVAGDQLLKGAALNAV
                     QIAEAL"
     gene            complement(2141..2746)
                     /locus_tag="VM713_03345"
     CDS             complement(2141..2746)
                     /locus_tag="VM713_03345"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVH83332.1"
                     /translation="MPHGPLFPGHLISWVWAVFALYWLAAMIGNKATQRREPLVARLA
                     HVLMLVAGAVLLAWHGTPLAGWLSLRLWPRSLLLYWIGLALLVAGLGFSVWARVHLGR
                     NWSGTVTVKEGHELIRTGPYRYVRHPIYTGILTGVIGTVICAATVRAVLGLLIIAVAL
                     LRKLRAEERFMRETFPAQYQEYCEEVPALIPFTRPRRSAPR"
BASE COUNT          472 a          983 c          990 g          436 t
ORIGIN      
        1 agcgccgcgg tgctgacggg cgcgcccgag ctcgggttgg agctcggact gcgtgcctac
       61 cgcacccaca tcctctggaa cggcgcgatc gagtgccggc tgctgcggat cgaggtggga
      121 ccggagagcg agcgcgcgcc cggcacgctc ggcaggggag tggcacacct caaggacacg
      181 gccggagcgc gcatgttcgc caaccggctc accaagaacc tgaagaggtt gcgcggctgg
      241 gccgagcgct caggcgtgtc gtgttatcgg ctctatgagg cggacatgcc cgagtacgcc
      301 ttcgcgatcg acctctaccg cacggtggac acgcaagcgg attggctcta cgtgcaggag
      361 tactcggctc ccgccgagat cgagcgcgaa gcggtacagc gccgccgcgg cgaggtgctt
      421 gcgagcctgc ccgcggtggc gggtgtgccc cccgagcgca tccgcgtgcg cacccgccgc
      481 cgcacacggc gcggcgagca gtacgagaag ctcggcgagc ggcagcgctt tcacgtcgtg
      541 gccgaggggg gtctcaagtt tcgggtgaat tttgacgact acctcgacac gggactcttc
      601 ctcgatcatc gcctgacgcg cgcgcggctg cggagcgccg cgccggccaa gagcttcctc
      661 aacctgttcg cctacaccgg aaccgcgacg gtgtacgcgg ccgcgggggg cgcgcacgcg
      721 agcgtctcgg tcgacctgtc acgcacctat ctcgagtgga tgcagcagaa tctcgcgctg
      781 aacgggctcg cgggcgccgc ccacgccctg gtgcaggctg actgccgcga gtggcttgcc
      841 ggggcgggcg cgcgtgcccc gcgcttcgat ctgatattcc tcgatccgcc gaccttctcg
      901 aactccaagc gcatggcagg agtgctcgac atcgcccgcg atcaccgcca gttgatcgag
      961 gggtgcgtgc ggctgctggc tccggcggga ctcctggtgt tctcgaccaa cgcccagcgc
     1021 ttcaagctcg cgccggagct taccgagcgc ttctccatcg tcgatgtctc ggcggcgacc
     1081 ttacctgagg acttcgcacg caatccgcgc attcaccgtt gttacgagtt cagggtaaga
     1141 tgattcaaca tgccatctga gcgcagatcc aagccagccg tagtcgccat cgtcggggct
     1201 accggcgccg tgggcgtgga gatgatcggc tgcctcgagc gccggcgctt cccgctcgcc
     1261 gagctgcgcc tgctcgcttc ggcgcgctcg gcgggcaaga cgctcgagtt tcgcggcgcg
     1321 ccgctcacgg tacgtgagct gaccgaggcg agctttgccg gcgtcgacct cgcgctgttc
     1381 tctgcgggca gcgccgtcgc gaagaagttt gcgccgctcg cggcgcgcgc tggcgcgatc
     1441 gtgatcgaca actcctcggc gttccgcatg gatcccgagg tgccgctggt ggtgccagag
     1501 atcaaccagc aagccgctcg cgcgcaccgt gggatcatcg ccaatcccaa ctgcaccgcc
     1561 atcatctcgg ccacgccgct gtggccggtg aaccgggcga accgcatcgt gcgactgctc
     1621 atcgcgagct accagtcggc ctcgggtgcg ggcgctgccg cgatggagga gttgcgcgag
     1681 tcgacgcgcg cctacctcga ggggcgttcc ttcgagccgc gggtgctgcc acacccctac
     1741 gccttcaacg tcttcagtca caacgcgcgc gtcgatccgg cgtccggcta caacgaggag
     1801 gagatcaagg tgagggacga gacgcgcaag atcttcggcg acccggaact gagagtatcg
     1861 gcgacctgcg tacgggtgcc ggtgctgcgc gcgcactcgg tcgcgatcaa cttcgagtgc
     1921 cagcgaccga tcacgcccga ggaggtccgc cgcctgatcg ccgacgcgcc gggcgtgaag
     1981 ctcgtcgatg accgcgagcg caattatttc ccgatgccgc gcgatgcttc cggaggcgat
     2041 ccgatcctcg tcggacgcat ccgccaggat gtgagcgatc cgagcggccg ctcgatcgca
     2101 ctcttcgtag cgggcgatca gctcctgaag ggcgccgcgc tcaacgcggt gcagatcgcc
     2161 gaggccttgt gaaggggatg agcgccggaa cctcctcgca gtactcctgg tactgagcgg
     2221 ggaaggtctc gcgcatgaag cgctcctcgg cgcgcagctt gcgcaggagc gccaccgcga
     2281 tgatgagcag gccgagcacg gcgcgcaccg tcgccgcaca aatcaccgtg ccgatcacgc
     2341 ccgtgagaat gccggtgtag atcgggtgcc gcacgtaccg gtacggcccg gtgcggatca
     2401 gctcgtgtcc ttccttgacg gtcaccgtgc cactccagtt gcgcccgagg tgcacgcgcg
     2461 cccacacgga gaatccgagt cccgcgacga gcagcgcgag gccgatccaa tagagaagga
     2521 gtgagcgcgg ccagagccgc agcgacagcc agcctgccag cggcgtgccg tgccacgcga
     2581 gcagtactgc gccggccacg agcatcagaa cgtgcgcgag gcgcgcaacc aagggctcgc
     2641 gccgctgcgt cgccttgttg ccgatcatcg cggcgagcca gtagagcgca aagacggccc
     2701 acacccatga aataaggtgt ccggggaaga ggggaccgtg cggcactaca acaggtcccg
     2761 cagccggtaa tacagcatcg cgagcacgag cgccggacgg cgcagcgcgg tgccgcccgg
     2821 gaagctgccg tgcgggatgc gtgcgaacac gtcaaagcgc gcggccgtgc cggcgaccgc
     2881 c
//
LOCUS       DATLKL010000258         2876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_59358, whole genome shotgun sequence.
ACCESSION   DATLKL010000258 DATLKL010000000
VERSION     DATLKL010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59358"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VM713_03350"
     CDS             <1..844
                     /locus_tag="VM713_03350"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HVH83333.1"
                     /translation="RATGFAGDFAQFLHFLRTDPRFYAKTPQELLLRAAWIAKVFDGK
                     ASTYFGYLPRARFAIKPVPDDLAPFYTAGRGGPGVYLLNTYDLPSRPLYNLAALTLHE
                     SAPCHAFQIPIALEHEGQPDFRRHSYISAYGEGWALYCEKLGEEMGMYETPYDRFGML
                     GYQIWRAARLVVDTGVHAEGWTREQALDYLRRYTALPEREIGTEVDRYIAWPGQALSY
                     YLGERSILEGRARAERALGEGFNIRAFHDAVLELGSVPLPVLSARINRFIAEGGKGPY
                     PDME"
     gene            904..1263
                     /locus_tag="VM713_03355"
     CDS             904..1263
                     /locus_tag="VM713_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HVH83334.1"
                     /translation="MKRTRWSVAVGAALAVLAGTAVFAQAAGTTTAAKPANSQQEKMR
                     SCNAEATAKGSHGAGRKSFMSTCLSDQPAAPAKTNSQQEKMKSCNADATAKGLKGADR
                     KTFMSSCLKSSGSEASK"
     gene            1312..1797
                     /locus_tag="VM713_03360"
     CDS             1312..1797
                     /locus_tag="VM713_03360"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVH83335.1"
                     /translation="MTDAQVPVATGGCTCRAVRYTLRGKPLIVHCCHCRWCQRETGSA
                     FVLNALIEADRVALLAGAPEVVMTPSASGKGQKIARCPVCRIAVWSTYAGAGDVLRFV
                     RVGTLDDPDAFPPDIHIFTESRQPWVVIPAGACAVSQYYKSAEVWSEESLARRRALLG
                     K"
     gene            1894..>2876
                     /locus_tag="VM713_03365"
     CDS             1894..>2876
                     /locus_tag="VM713_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVH83336.1"
                     /translation="MRSPTPAEQYPFAGQQLPCYVAGEFIRSAQSFPNTNPVNGRVIA
                     TVSEADAALVDRAVQAARAALRGPWGRLTIQERCGLLRKVAERIEQRFDEFLSAEIAD
                     SGKMLAQAKSLDIPRGAANFRSYADLALARAAECYEMATPDGRGALNYTVHKPVGVVA
                     AIAPWNFPFLLLTWKVAPALVCGNTVVAKPSEETPSSAVLLAQVMQEVGIPPGVFNLV
                     HGFGPASTGEALVSHPDVNAIAFTGETATGAAIMRSAAPTVKALSFELGGKNAALVFA
                     DADFEAAVAGTMRSVFSNCGQVCLCSERVYVERPIFERFVDALAARARALK"
BASE COUNT          479 a          950 c          980 g          467 t
ORIGIN      
        1 gcgcgccacg ggcttcgccg gcgacttcgc gcagttcctc cacttcctgc gcaccgatcc
       61 gcgcttctac gccaagacgc cgcaggagct gctgttgcgc gccgcgtgga tcgccaaggt
      121 gttcgacggc aaggcgtcga cctacttcgg ctacctgccg cgggcgcgct ttgcgatcaa
      181 gccggtgccg gatgacctcg cgccgtttta caccgcgggc cgcggcggcc cgggcgtata
      241 ccttctcaac acctacgacc tgccgagccg cccgctctac aatctcgcag cgctcacgct
      301 gcatgagtcg gcaccgtgtc atgcgttcca gatcccgatt gcgctggagc acgagggcca
      361 gccggacttc cgccgtcaca gctacatctc ggcgtacggc gagggctggg cgctctactg
      421 cgagaagctc ggtgaggaaa tgggcatgta cgagacgccc tatgatcggt tcgggatgct
      481 cggctaccag atctggcgcg ctgcgcggct ggtggtcgac accggagtcc acgccgaggg
      541 ctggacgcgc gagcaggcgc tggactatct gcgccgctac accgcgttac ctgagcgcga
      601 gatcggcacc gaggtggacc gctatatcgc ctggcccggg caggcgctgt cctattacct
      661 cggtgagcgc tcgatcctcg aaggacgcgc gcgcgccgag cgcgcgctcg gtgagggctt
      721 caacatccgc gccttccacg atgcggtgct cgagctcggc tcggtgccgt tgccggtcct
      781 gtcggcacgc atcaaccgct tcatcgccga gggcggcaag ggaccctatc cggacatgga
      841 gtagtggacc gtctggggat aggataccgc cgcattcaat ttcaccagga aggggaccga
      901 cacatgaaac gtacaagatg gtcagtggcg gtgggggcag cgctcgcggt tctggccggg
      961 acggccgtct tcgcgcaggc ggcgggcacg acgaccgcgg ccaagcccgc caacagccag
     1021 caggagaaga tgaggagctg caacgcggaa gcgacggcca agggatcgca tggcgccggt
     1081 cgcaagagct tcatgagcac ctgcctgtcc gaccagcccg cggcgccggc caagaccaac
     1141 agccagcagg agaagatgaa gagctgcaac gcggacgcga ctgccaaggg actgaagggc
     1201 gcggatcgca agactttcat gagcagctgt ttgaagagct cgggatccga ggcgagcaag
     1261 taagaggccg gcgccggttc gctcacccgt gggccggtgc cgttcccgtg aatgactgac
     1321 gcacaagtgc ctgtggcgac gggcggctgc acctgccgcg ccgtgcgtta cacgctgcgg
     1381 ggcaagcctc tcatcgtgca ctgctgtcat tgccgctggt gtcagcgcga gaccggctcg
     1441 gcgtttgtgc tgaatgccct gatcgaggcg gatcgcgtcg ctctcctggc gggagctccg
     1501 gaggtggtga tgacaccgag cgcgagcggc aagggccaga agatcgcccg ctgcccggtg
     1561 tgccgcatcg ccgtctggag cacctacgcg ggcgccgggg atgtgctgcg tttcgtgcgc
     1621 gtcggcacgc tcgatgatcc cgatgccttc cctcccgaca tccacatctt caccgagtcc
     1681 aggcaaccgt gggtggtgat tccggccggg gcgtgcgcgg tgagccagta ttacaaaagt
     1741 gctgaggtct ggagcgagga gagcctggcg cggcgacggg cgctgctcgg gaagtgacgc
     1801 gggagcgtcg gcacaggtat agaggaaacg cataccagag gtagaaaaaa cgattattcc
     1861 ttggtagcag ccgcccccta aaatccgcgc cggatgcgca gccccactcc agctgaacag
     1921 tacccgttcg cgggccagca gttaccctgt tatgtcgccg gcgaattcat ccgctccgct
     1981 cagagcttcc ccaataccaa cccggtaaac ggccgggtga tcgccaccgt cagcgaggcc
     2041 gacgcggcgc tggtcgatcg cgccgtacag gcggcacgtg cggcgctgcg cggaccctgg
     2101 ggccggctca cgatccagga gcgctgcggg ctgctgcgca aggtggccga gcggatcgag
     2161 cagcgcttcg atgagttctt gagcgccgag atcgccgatt ccggcaagat gctcgcgcag
     2221 gccaagtcgc tcgacatccc gcgcggcgcg gcgaacttcc gctcctacgc cgatctggcg
     2281 ctggcgcgcg ctgccgagtg ctacgagatg gccaccccgg acggacgcgg cgcgctcaac
     2341 tacacagtgc acaagccggt cggcgtcgtg gcggcgatcg ccccgtggaa cttccccttt
     2401 ctgctcctca cctggaaggt ggcgccagcc ctcgtctgcg gcaacacggt ggtcgccaag
     2461 ccctccgagg agacgccgtc ctcggcggtg ctgcttgcgc aggtcatgca ggaagtgggc
     2521 atacccccgg gagtgttcaa cctggtgcac gggtttgggc ccgcctcgac cggcgaggct
     2581 ctggtcagcc atcccgacgt gaacgcgatc gccttcaccg gcgagactgc gaccggtgct
     2641 gccatcatga gaagcgccgc cccgacggtg aaggccctgt ctttcgagct cggcggcaag
     2701 aacgcggcgc tcgtgttcgc ggacgccgac ttcgaggcgg ccgtcgccgg cacgatgcgc
     2761 tcggtgttct cgaactgcgg ccaggtgtgc ctgtgctcag agcgggtcta cgtcgagcgt
     2821 ccgatcttcg agcgcttcgt cgatgcgctc gccgcccgcg cgcgtgcgct caagat
//
LOCUS       DATLKL010000259         1876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_59361, whole genome shotgun sequence.
ACCESSION   DATLKL010000259 DATLKL010000000
VERSION     DATLKL010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59361"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VM713_03370"
     CDS             <1..294
                     /locus_tag="VM713_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83337.1"
                     /translation="NVIVGSNIGRLGKKADNDDMRCVFTGRPNLEVNSTGKRFESEMQ
                     LHDRAGNVIGALGVVYAYAKGADKEALHARAEAVRRELEKRIPDAAGLFGPAA"
     gene            329..1357
                     /locus_tag="VM713_03375"
     CDS             329..1357
                     /locus_tag="VM713_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83338.1"
                     /translation="MSAAGAIACAASADAAAPQAAAPGAVLVLAGHTELPGYSGDFDH
                     FAADVAGGRLFLAAEDHGTLEVFDLHSGRHLKSVSGFETPHSLFLVPQAHRLLITDGS
                     DSIKLLDDRTLAPAGVIRLHPGADSIGFDGSNGHLYVVTGGKDVKLKESWLEEIDPVS
                     TRKIGEVHLDADHVEAMAVEQHGPHIYLNVTDRNYVAVIDKGTRTIVARWAIHEAAQN
                     ALAQLYEAARRLFIVARQPGRFVVLDADSGASVASFPAPGRVDAEIFDAANHRIYAPG
                     GEGWIGVYAEHDGGHFAELAHVPSQVGAKTAILVPELHRLYVAVSPGEGKTGAAIIWF
                     DVLSSPAS"
     gene            1399..>1876
                     /locus_tag="VM713_03380"
     CDS             1399..>1876
                     /locus_tag="VM713_03380"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVH83339.1"
                     /translation="MDKHNNPLLITVVGAAAVLGFADAHAQQAAEAAPAAAAQAPLAE
                     IVITGSSIPTTPDTVAVPVETLTAAQLQASGVDSNALEILRKEIPAFQGRSNAGTSNA
                     NNDNQRTAGGSQLQLRNLPTLILVNGRRVANSGVGGINGKNFVDVNQIPAAAIDHIE"
BASE COUNT          345 a          601 c          646 g          284 t
ORIGIN      
        1 aacgtcatcg tcggctcgaa catcgggcgg ctggggaaga aggccgacaa cgacgacatg
       61 cgctgtgtct tcaccggcag gccgaacctc gaggtcaatt ccaccggcaa gcgcttcgag
      121 agcgagatgc aattgcacga ccgggccggc aacgtgatcg gcgccctcgg tgtcgtctat
      181 gcctatgcca aaggcgcgga caaggaggcg ttgcacgccc gcgcggaagc ggtccgcagg
      241 gagctggaga agaggattcc cgatgcggct ggcctgtttg gtccggccgc ctgaagcgag
      301 gaggagtatg cggctagcga gattgctgtt gagcgcagcc ggggccatcg cctgtgccgc
      361 gagcgcggac gccgcggcac cgcaggccgc ggcgccgggc gcggtgctgg tgctcgcggg
      421 ccacacggag ctgcctggct acagtgggga tttcgatcac ttcgccgcgg acgtagcggg
      481 gggccggctg ttcctggcgg ccgaggatca cggcacgctc gaggtcttcg atttgcacag
      541 cggcaggcac ctgaagagcg tgagcggctt cgaaacaccg cacagcctat ttctcgtgcc
      601 gcaggcgcac aggctcctga tcacggacgg cagcgacagc atcaagctgc tcgatgacag
      661 aacgctagcg ccggccggcg tgatccgcct ccacccgggc gccgactcca tcggtttcga
      721 cggcagcaac ggccacctct atgtcgtcac tggcggcaag gacgtcaagc tgaaggagtc
      781 gtggctcgag gagatcgatc cggtatcgac acgcaagatc ggcgaggtgc acctggacgc
      841 cgaccacgtc gaggccatgg cggtcgagca gcacgggccg cacatctacc tcaacgtcac
      901 cgaccgtaac tatgtagcgg tgatcgacaa gggcacgcgc acgatcgtcg cccgctgggc
      961 gatccacgag gcggcacaga atgcgctggc gcaactctac gaggcggccc gccggctgtt
     1021 catcgtcgcg cgccagccgg gcaggttcgt cgtcctcgac gcggacagcg gtgccagcgt
     1081 ggcgagcttc cctgcacctg gacgggtcga cgcggagatc ttcgatgcgg cgaaccatcg
     1141 tatatatgcc ccgggcggcg agggctggat cggcgtctac gcggagcacg acggtggaca
     1201 ctttgccgag ctcgcgcatg tgccgagcca ggtcggtgcc aagactgcga tcctggttcc
     1261 ggagctgcac cgcctgtacg ttgccgtgtc cccgggcgag ggcaagaccg gtgcggcaat
     1321 catctggttc gacgtgttga gcagtccggc ttcatgacga gcggccgcgt ggctggccgt
     1381 cgcaggggag gagtcatcgt ggacaaacat aacaatccgc tgctgatcac cgtggtaggc
     1441 gcggccgcag tcttaggttt cgccgacgcc cacgcgcagc aggccgccga ggctgcgccg
     1501 gctgctgccg cccaggcacc gctggcggag atcgtcatca ccgggagcag catcccgact
     1561 actccggaca ccgttgcagt gccggtcgag acactgaccg cggcgcagct gcaggcgagc
     1621 ggcgtggact cgaatgcgct ggagatactg cgcaaggaga ttcccgcgtt ccagggccgc
     1681 agcaatgccg gcacgagcaa cgccaacaat gacaaccaac gcacggccgg cggctcgcag
     1741 ttgcaactgc gcaatctgcc gaccctgatc ctggtcaacg gccgacgggt cgcgaatagc
     1801 ggtgtcggtg gcatcaacgg caagaacttc gtcgatgtca accagatccc ggccgccgcg
     1861 atcgatcaca tcgaag
//
LOCUS       DATLKL010000260         2277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_14062, whole genome shotgun sequence.
ACCESSION   DATLKL010000260 DATLKL010000000
VERSION     DATLKL010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14062"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..2125
                     /locus_tag="VM713_03385"
     CDS             137..2125
                     /locus_tag="VM713_03385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HVH83340.1"
                     /translation="MKLTRLLFVAAVSLLTTLAAAATKEAGTPTMAPENPFAHPSALP
                     FELPPFDRIHDADYTPAFEAGMREQLKEVAAIAHDPHPATFENTIVALERSGRLLDRV
                     GSVFYNLNSCNTDPQMEEIDTVMAPKLAAHNDAIFLDAALWARVAALYGKRENLGLDA
                     ESLQLLARYHTQFVRAGAQLSGADQQRLRAINEELSTLTTRFRQNVLKATADGAVVVD
                     KVADLEGLTPVQIGAAAQAAADRGHKDKWVIALQNTTNQPMLASLTNRDLRARIYRAS
                     IGRGLSGATDNREVIAQLVKLRAERARLLGYPDHAAYVLEDESAGNPQAVAAMIREVA
                     PAALKRAREEAADIQKLIDAQAAARGVPSFTLQPWDWSFYAEQVRKAHYDFDQAQVAP
                     YFELERVMRDGLFYAAHELYGLSFKERKDLPVYQPDVRVFEVSDADGKPLALFIADYF
                     ARDNKQGGAWMNSYVSQSKLLGQRPVVANHLNIPKPQPGQPVLLTFDEVTGMFHEFGH
                     AIHGMLSNVRYPLLSGTAVPRDFVEYPSQYNEMWAREPQVVAHYANDYRSGAPMPPEL
                     LAKVLAAQKFNQGYATLEYVEAAQVDLAWHLIRGAQAPAAAAVPGFEAAALKDAGLDY
                     APVQPRYHSGYFSHIFAGGYSAGYYAYLWSEVLARDTG"
BASE COUNT          392 a          790 c          748 g          347 t
ORIGIN      
        1 agcgtgtaat gaatggtaac gacgtcgttt tgcgcgatgg gcatcggggt tgtccggctc
       61 gaaaagctgc gcagcgggcc cgcggagaat accattagcg cccggccgag ccagggatgg
      121 gctggaggaa aggtccatga aactcacccg attgctgttc gttgccgccg tgagcttgct
      181 gaccacgctc gcggccgccg caaccaagga agcaggaacc cccacaatgg cccccgagaa
      241 ccccttcgcc cacccgagcg cactgccgtt cgagctgccg cccttcgacc ggatccacga
      301 cgccgactac acgcccgcct tcgaggccgg catgcgtgag cagctcaagg aggtcgcggc
      361 gatcgcccat gatccacacc ctgccacctt cgagaacacg atcgtcgcgc tcgagcgctc
      421 cggacggctg ctcgaccggg tggggagcgt gttctacaac ctgaactcgt gcaataccga
      481 tccgcaaatg gaggagatcg ataccgtcat ggcgccgaag ctcgccgcgc acaacgacgc
      541 gatcttcctc gacgcggcgc tgtgggcgcg cgtcgccgcg ctatacggca agcgcgagaa
      601 tctcgggctc gatgctgaat cgctgcagct gctggcgcgc taccacacac agttcgtgcg
      661 cgccggcgcg cagctctccg gggccgatca gcagcggctg cgggccatca acgaagagct
      721 ctcgaccctc accacgcgct tccgccagaa cgtgctcaag gcgaccgccg acggcgccgt
      781 ggtggtcgac aaggtcgcgg atctcgaggg gctcacgccg gtgcagatcg gagccgcggc
      841 gcaggcggcg gccgaccgcg gacacaagga caagtgggtc atcgcgctgc agaacaccac
      901 caaccagccg atgctcgcaa gtctcaccaa ccgtgatttg cgcgcgcgta tctaccgcgc
      961 ctccatcggc cgcggcctct ccggggccac cgacaaccgc gaggtgatcg cgcagctggt
     1021 gaagctgcgc gcagagcgcg ccaggctcct cggctacccc gatcacgcgg cctacgtgct
     1081 cgaggacgag tctgccggga acccgcaggc ggtcgccgcc atgatccgcg aggtggctcc
     1141 ggccgccctg aagcgcgcgc gcgaggaggc cgccgatatc cagaagctga tcgatgcgca
     1201 ggcggccgcg cgcggcgtgc cctcctttac gctgcagccc tgggactggt cgttctacgc
     1261 cgagcaggtg cgtaaggcgc actacgactt cgaccaggcg caggtggcgc catacttcga
     1321 gctcgagcgc gtgatgcgcg atgggctgtt ctacgcggcg catgagctct acgggctcag
     1381 cttcaaggag cgcaaggacc tgccggttta tcagcccgat gtgcgcgtgt tcgaggtctc
     1441 ggacgccgac ggcaagccgc tcgcgctgtt tatcgccgac tacttcgcgc gcgacaacaa
     1501 gcagggcggg gcgtggatga acagctacgt gtcgcagtcg aagctgctcg gccagcggcc
     1561 cgtggtcgcc aaccatctca acattcccaa gccccagccg gggcagccgg tgctgctcac
     1621 cttcgatgag gtcacgggaa tgttccacga gttcggccac gccattcacg gcatgctctc
     1681 gaacgtccgc tatccactcc tgtcgggcac cgcagtgccg cgcgacttcg tcgagtaccc
     1741 ctcgcagtac aacgagatgt gggcgcgcga gccgcaggtg gtcgcccact atgcgaacga
     1801 ctaccgcagc ggcgcaccga tgccaccgga gctgctcgcc aaggtgctcg cggcgcagaa
     1861 gttcaaccag ggctatgcga cgctcgagta cgtcgaggcg gcgcaggtcg acctcgcctg
     1921 gcacctgatc cgtggggccc aggcgccggc ggccgcggcc gtgccgggat tcgaggcggc
     1981 ggcgctcaag gacgcggggc tcgactacgc cccggtccag ccgcgctatc actcgggcta
     2041 cttctcgcac atctttgccg gcggctattc cgccggctac tacgcctatc tctggagcga
     2101 ggtgctggcg cgcgacaccg gctagtggtt ccacgcgcat ggcgggctga cgcgcgagaa
     2161 cggcgatacg ctgcgccgca tggtgctgtc gcgcgggcgc accgaggatc cgcaggtcct
     2221 gttccgcaac ttctacgggc acgcgcccga aatcggaccg ctgctcgaat accgggg
//
LOCUS       DATLKL010000261         2117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_115840, whole genome shotgun sequence.
ACCESSION   DATLKL010000261 DATLKL010000000
VERSION     DATLKL010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115840"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VM713_03390"
     CDS             <1..1075
                     /locus_tag="VM713_03390"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVH83341.1"
                     /translation="CTALLYLLPWSGTTNARGDYVITTSARAVQHVMVFIVAAVAYRI
                     ALAVGWPEGVLARSRVVIINTLLALAVAAWSEAALALVAGFVDGHMADMRDTFTAMKG
                     TASNLRLAAGPLQMFLPPYVLGLCAIALVLVAERHHREALRAAELARAYTTARMAMLS
                     AQLQPHFLFNSLHAAMGLIDENPRQASIMLARLGDFLRHALETSRSPWVDVATELAVL
                     EAYLAVQQTRFSDHLNIAIDASPDSLGVYLPSMLLQPLAENAIEHGRREGAPLELRVA
                     ASVSAEHLCIVVKNSSPQLKADLTPADYGHGLSNVDLRLRAAYGDEAHLAVGPDQQGG
                     TTAVLLLPVRRRGCAESPEGVPA"
     gene            1072..1830
                     /locus_tag="VM713_03395"
     CDS             1072..1830
                     /locus_tag="VM713_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HVH83342.1"
                     /translation="MTSAPIPTFVVDDEPHARKRLLHLLRGDPDIRVVGTFASATAAA
                     SRARELTPQLLLLDIRMPELDGFELVAALAGHGVNPYIIFVTAHSDRSMDAFGVGAVD
                     YLLKPFDDERFARAIERAKKLIGSGGQPRAASPQQPASLASNRTRLLLSERGKVVVLA
                     MRDIEFVQAAAKHVKIYAAGRCYSFRQTLGELESRLDPASFVRVHRSTLVNVEHLAEM
                     HPLFHGDYELILRRGTRLTLSRRYRDRLTPFLVE"
BASE COUNT          304 a          760 c          692 g          361 t
ORIGIN      
        1 ctgcacggcg ctcctgtacc tgctgccgtg gagcgggacc acgaacgcca ggggcgatta
       61 cgtcatcacg acttccgcgc gcgccgtcca gcatgtcatg gtgttcatcg tggcggccgt
      121 tgcctatcgc atcgcactcg ccgtcggatg gcccgagggc gtcctggcac gcagccgagt
      181 cgtgatcatc aacacgctgc tggcgctcgc agtggctgcc tggtccgagg ccgcactggc
      241 gcttgtggcc gggttcgtcg acgggcacat ggccgacatg cgtgacacct tcaccgcgat
      301 gaagggcacg gcctcgaacc tcaggctggc cgccggcccg ctgcagatgt tcctgccgcc
      361 gtacgtcctc ggcctgtgcg cgatcgcgct ggtgctggtc gccgagcgcc accaccgcga
      421 ggcgctgcgc gcggcggagc tggcgcgtgc ctataccacg gcacgcatgg cgatgctgtc
      481 ggcgcagctg cagccgcatt ttctcttcaa ctcgctgcac gcggcgatgg gactgattga
      541 cgagaatccg cggcaggcct cgatcatgct ggcgcggctc ggcgacttcc tgcgccacgc
      601 gctcgagacc agtcgctcac cctgggtcga tgtcgccacc gagcttgcgg tgctcgaggc
      661 ctatctcgcc gtccaacaga cccgcttctc ggatcatctt aacattgcga tcgacgcgag
      721 cccggactcg ctcggggttt acctgccgtc gatgctgctg cagccgctcg ccgagaacgc
      781 catcgagcac ggccgccggg agggtgcgcc gctcgagctg cgcgtggccg cttcagtctc
      841 agccgagcat ctctgcatcg tggtgaagaa ctcgagtccg cagctcaagg cggatctgac
      901 gccggcggac tacggtcacg gcctctcgaa cgtggacctg cgcctgcgcg ccgcgtacgg
      961 ggacgaggcg catctggcgg tcggacccga ccagcagggt ggcacgactg cggtcctgct
     1021 gctgcccgtg cgccgccggg gttgtgcaga atcgccggag ggcgtgccgg catgaccagc
     1081 gccccaatcc ccaccttcgt ggtggacgat gagccccatg cgcgcaagcg actgctccac
     1141 ctgctgcgcg gcgatcccga catccgcgtc gtcggcacgt tcgcctcggc caccgccgcc
     1201 gcgagccgcg cgcgcgaact gacaccgcag ctcctgctgc tcgacatccg catgcccgaa
     1261 ctcgatggct tcgagctcgt cgcggcgctc gccggacatg gcgtcaaccc ctacatcatc
     1321 ttcgttacgg cgcattcgga ccgctcgatg gacgccttcg gcgtcggggc ggtcgactac
     1381 ctcctcaagc cctttgacga cgagcgcttc gcgcgcgcta tcgagcgcgc caagaagctg
     1441 atcgggtcgg gcggccagcc gcgcgcggct tccccgcagc agccggcctc gctcgccagc
     1501 aaccgcacgc ggctgctgct ctccgagcgc ggcaaggtgg tggtgctggc gatgcgcgac
     1561 atcgagttcg tgcaggcggc tgccaagcac gtcaagatct acgccgcggg ccggtgctac
     1621 tcctttcgcc agacgctcgg tgagctcgag agccgcctag accctgcgag cttcgtgcgc
     1681 gtgcaccgct cgacgctggt caatgtggag catctcgccg aaatgcaccc gctgttccac
     1741 ggcgattacg agctgatcct gcggcgcggc acgcggttga cgctcagccg ccgttaccgc
     1801 gaccgcctga cgccgttcct ggtcgagtaa gcgtccccgc gaggcgcaaa tccaggtgcc
     1861 gcggcgttgc aagcgcccca cgcgcgccgc aatcgcgcga cattcacccc gctcagcgca
     1921 ctgctgactc cgcttgaacc gcgagccgct ccgcttgcgc ctgtggggtt gcgggtacgt
     1981 cgcgacaatc gctacggtct ctgcacggtg acagcgtgca cacgggaaac catcctgtcc
     2041 tcggtgcttc acaattgact gggccgaggc ggacgttcag ggccggggtg tcccgtgcgc
     2101 aggacgtgct cttcctg
//
LOCUS       DATLKL010000262         2899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_14071, whole genome shotgun sequence.
ACCESSION   DATLKL010000262 DATLKL010000000
VERSION     DATLKL010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14071"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VM713_03400"
     CDS             <1..415
                     /locus_tag="VM713_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVH83343.1"
                     /translation="ARYADWEPGQCRKSTRGEGESDMPESIYPPTTADLARRRRELAP
                     DIQAAFDAFSQRVFADGALPAKTKQLIAVAVAHVTQCPYSIRGHTKAAMRQGATANEL
                     MEAIWVAAEMRAGGAYAHSALSLATMDEQGRGKTP"
     gene            485..2023
                     /locus_tag="VM713_03405"
     CDS             485..2023
                     /locus_tag="VM713_03405"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH83344.1"
                     /translation="MGESASRLSGPDLGQGIPLAKIADGTTVLGHVRGEAALLVRRGD
                     ELFAIGATCTHYGGPLADGLLVDDTVRCPWHHACFSLRTGDALRAPALNALSRWRVEQ
                     RDGIVYVGEKFGKPEPRTLPTVTDLPKSVVIVGGGGAGSAAAEALRREGYSGRITMLS
                     ADEFPPYDRPNLSKDYLAGNAPEEWIPLRSPEFYGKHGIDLRLCAQAIAIDLRAREVQ
                     LEDGSRHRYDKLLLATGAEPVRLGVPGAELPHVHYLRMLADSRALIARAKASRRAVVI
                     GASFIGLEVAASLRARGIELHVVAPETRPMERVMGAEIGDLIRAIHEEHGVVFHLGTT
                     VVAIDKTSVTLKTGERLAADFVVVGIGVRPETALAERAGLAIDRGIAVNEYLETSVPS
                     IFAAGDIARWPDRITGDRIRVEHWVVAERQGQTAARNMLGLHERFDAVPFFWSQHYDV
                     AISYVGHAERWDRVEIDGKPEARDCKATFRRGDRKLAVVTISRDIESLRAEVEFEQLA
                     VGSR"
     gene            complement(2166..>2899)
                     /locus_tag="VM713_03410"
     CDS             complement(2166..>2899)
                     /locus_tag="VM713_03410"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVH83345.1"
                     /translation="GIDPSSVEGLADTPYGVPNRRFEYVMRHEAVPVGFWRSVGNSQN
                     AFFAESFIDEMAHAAKQDPVAFRLALLADKPRHRAVLEKAAQTAGWGTAAPAGRFRGV
                     ALHESFGSIVAEVAEISLDGGKTLRVHKVTCAIDCGRAMNPDTVVAQMESGVVFGLTA
                     AFYGEIMIKDGRVDQANFDTYPMLKLAQTPAIEVHIVESGAKLGGIGEPGTPPIAPAV
                     ANALFAATGTRIRSLPLVKNGIEPA"
BASE COUNT          496 a          938 c          993 g          472 t
ORIGIN      
        1 cgcccgttat gcggattggg aacccggcca gtgccggaaa tccacgagag gcgaaggaga
       61 aagcgatatg ccggaatcga tctatccgcc tacaaccgcg gacctcgcac gcaggcgccg
      121 cgagcttgcg cccgacatcc aggcagcgtt cgacgcgttc agccagcgcg tattcgccga
      181 cggcgcgctg ccggccaaga ccaagcagct catcgccgtg gcggtcgccc acgttaccca
      241 gtgtccctat tctatcagag ggcacaccaa ggcggcgatg cggcagggag caacagccaa
      301 cgagctgatg gaggcgatct gggtggccgc cgagatgcgg gcaggaggcg cttacgctca
      361 ttccgcgctg tcccttgcta cgatggacga gcagggccgc ggcaagacgc cgtgaggtac
      421 gtaagtacgc aacttgcgcg caacggccaa agccttggca ccgcattagc tgaaggagga
      481 cggcgtgggc gaatcagcat cccgactcag cggacccgac ctcggccaag ggatccccct
      541 ggcgaaaatc gcggatggaa cgaccgtgct gggacatgtt cgcggcgagg cggcgcttct
      601 cgtgcgccgg ggcgacgagc tgttcgcgat cggcgccacg tgcacacatt atggcggccc
      661 acttgcggac gggcttctcg tcgatgacac cgtccgctgc ccctggcatc acgcctgctt
      721 cagcctgcgc accggcgacg ccctgcgtgc gccggcgctg aacgcgcttt cgcgctggcg
      781 ggttgagcag cgtgacggga tcgtgtatgt tggcgaaaag ttcggcaagc cggagccgcg
      841 aacgctgccg accgtcacgg acctgccaaa gtcggtcgtg atcgttggag gcggcggggc
      901 agggagtgcc gccgcggagg cgctccgtcg ggagggctac tcgggccgca ttacgatgct
      961 gagtgccgac gagttcccgc cctatgaccg gcccaacctg tcaaaggatt accttgcggg
     1021 taacgcgcca gaggagtgga ttccgctccg ttcgcccgag ttctatggaa agcatggcat
     1081 cgatctgagg ctgtgcgcac aggccatcgc gatcgacctt agggctcgcg aggttcaact
     1141 cgaggacggc agccggcatc gctacgacaa gctccttctc gcgacggggg ccgagccggt
     1201 gcggcttggc gtccccggcg ccgagcttcc gcatgtccac tatctgcgca tgctcgccga
     1261 cagccgcgcg ctcatcgcca gggccaaagc atcgcgccgc gcggttgtca tcggagcgag
     1321 ctttatcggc ctcgaagtcg cggcgtcgct gcgcgcccgc ggaatcgagc tgcatgtcgt
     1381 cgcgccggag acgcgcccga tggagcgtgt catgggcgcc gagatcggag acttgatccg
     1441 agcgattcat gaggagcacg gcgtggtatt ccatctcggc accacggtcg tcgcgatcga
     1501 caaaacgagc gtcactctca agaccgggga aagactagcg gcagatttcg tcgtggttgg
     1561 cattggggtg cggccggaga cagcgctggc cgaacgggcg ggactcgcga tcgatcgcgg
     1621 catcgcggtg aacgagtacc ttgagacgag cgtgcccagc atctttgccg ctggcgatat
     1681 cgcgcggtgg cccgaccgga tcaccggcga tcggattcgc gtcgagcatt gggtcgtcgc
     1741 cgaacgtcaa gggcagaccg cggcgcgcaa catgctcggc ctccacgaac gcttcgatgc
     1801 agttcccttt ttctggagcc agcactacga cgtcgcaatt agctacgtcg gtcacgccga
     1861 gcgctgggac cgtgtggaga tcgacgggaa gcccgaggcg agagactgta aagcgacatt
     1921 tcgccgcggc gaccgaaaac tggcggtcgt cacgatttcg cgagatatcg aaagtcttcg
     1981 cgcagaagtc gagttcgagc aattggcggt ggggagccga tgaacgacct ccctcgagcc
     2041 tgcccgattc atgctccagc tctgggcggg acgccgtcaa cgaagggcgg gtcatgtcgg
     2101 ccggttcggc tgagcggcgc tcagctttcg tagccgtccg atgggtcgcg gccgcggggc
     2161 ctgctctaag ccggctcgat gccgtttttc acgagcggga gcgagcggat gcgcgtgccg
     2221 gtcgccgcga acagcgcgtt cgcgacggcg ggcgcgatcg gcggcgtgcc gggctcgccg
     2281 atgccgccca atttggcgcc gctctcgacg atatgcacct cgatcgccgg cgtctgggcg
     2341 agcttcagca tcgggtaggt gtcgaagttc gcctggtcga cgcggccgtc cttgatcatg
     2401 atttcgccgt agaacgcggc ggtgagcccg aagacgacgc cgctctccat ctgggccacg
     2461 accgtgtccg gattcatcgc gcgcccgcag tcgatcgcgc aggtcacctt gtgcacgcgg
     2521 agcgtcttgc cgccatcgag cgagatctcg gcgacctcgg ccacgataga gccgaaggac
     2581 tcgtgcaggg ccacgccgcg gaatcggccc gcgggcgccg ccgtgcccca gccggcggtc
     2641 tgggccgcct tctccagcac ggcccgatgg cgcggcttgt cggccagcag cgctaggcgg
     2701 aaggcgaccg ggtcctgctt cgccgcgtgc gccatctcgt cgatgaagct ctcggcgaag
     2761 aacgcgttct ggctgttgcc aaccgagcgc cagaagccga ccggcaccgc ttcatgccgc
     2821 atcacgtact cgaaccggcg gttcggcacg ccatagggcg tgtccgcaag gccctccacc
     2881 gaggacggat cgatgccgc
//
LOCUS       DATLKL010000263         3655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_93246, whole genome shotgun sequence.
ACCESSION   DATLKL010000263 DATLKL010000000
VERSION     DATLKL010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93246"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VM713_03415"
     CDS             <1..222
                     /locus_tag="VM713_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83346.1"
                     /translation="RQAEIVGLALLADNSTHESIAGTLRPRVSKQSVTESLAAARWRP
                     LLEAIRVFEETDWDRLSPLSDLRQAEAIV"
     gene            274..999
                     /locus_tag="VM713_03420"
     CDS             274..999
                     /locus_tag="VM713_03420"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HVH83347.1"
                     /translation="MYETLLALLVAHLVADFPLQPDWMIRRKRHPAVLGLHGLIVVGT
                     AAFALGGWPSTLLLILVGTHLAMDAVKVYLLKDSLRSFTVDQVVHLLVILGLALGFPG
                     TYAQGWWAALPGNFAAAYAIGLCLLGGVIASLMVGAIVIRKATMPFTGQLSGDISGLE
                     SGGTYIGWLERALVMLLILIGQPAGVGFLITAKSILRFGDVRDSAQRKLTEYIIIGTF
                     MSFGWGLLIAVLTQLAMRHWMAP"
     gene            complement(996..2342)
                     /locus_tag="VM713_03425"
     CDS             complement(996..2342)
                     /locus_tag="VM713_03425"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HVH83348.1"
                     /translation="MSASEQKLFVFAYIDTEWLPCGQLTLSEEGARLLSSSFAYGLRY
                     LLRPGALEVDPVSLSLRDKLAVRGKALFPPNKLPSFGGIRDAAPDAWGRRVIESRLKV
                     PANSLPESTYLVHAGSQRVGALDIRSSRDSSATPGFGTWNNLEYLMDAAQRIDEGLPV
                     PSPLEEIFAEGSALGGARPKATVRDDERVLWLAKFPSRKDALAVPVVETATLRLAAAA
                     GLSVPQVRLIQLGSRTVMLIRRFDRYWAKAGQDAPLAQEFLETAPAYGLAEKRLGFVS
                     ALTLLACDEMDSPDKSYGDLAQAVRRYCHPRVIRENNRELFERLIFNILVNNDDDHLR
                     NHGFVWDPRLPGWRLSPLYDVLPRASLSPERRLHLGVGPEGRIATLDNAFAGREMFTL
                     SAEAAAESIARIWRIVREWKVYFEEYQVGADQIEKIAPAFRHLDDLSTPALRKLIP"
     gene            complement(2347..2688)
                     /locus_tag="VM713_03430"
     CDS             complement(2347..2688)
                     /locus_tag="VM713_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVH83349.1"
                     /translation="MKITQSAVLSASQAAEAKKLGAALARLRIARGVKQDQAALRAGL
                     SRNTAYRMEKGDPGIAMGQILRYLDAVVPGKTLFELLSETDPALAALSAKEQRRRARD
                     LSKRELSELDF"
     gene            2888..>3655
                     /gene="hemW"
                     /locus_tag="VM713_03435"
     CDS             2888..>3655
                     /gene="hemW"
                     /locus_tag="VM713_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HVH83350.1"
                     /translation="MSALLVPPLSLYVHFPWCVRKCPYCDFNSYALQGELAAEPYVAR
                     LARDLEAQAPEVAGRPVESVFFGGGTPSLFSPESIARVLGAARRHLHLSADAEVTLEA
                     NPGAIERGAFGEYRDAGVTRVSLGAQSFDPAALGALGRIHSPAETRRAAEELHAASLT
                     NFNLDLMYALPGQERSGARSDVEAALALAPAHLSHYQLTLEPGTVFAAHPPPLPDEDE
                     AAAMQEECATLLAAAGFGHYEISAWARPGRQCRHNLNY"
BASE COUNT          578 a         1223 c         1223 g          631 t
ORIGIN      
        1 cggcaggccg agatcgtcgg cctcgcgctg ctcgcggaca attccaccca tgaatcgatc
       61 gccggcacct tgcgccccag ggtctcgaag cagagcgtga cggaatcgct ggctgcggcc
      121 cgctggcgtc cgttgctgga agcaatccgg gttttcgagg aaaccgactg ggacaggctc
      181 agcccattgt ctgatctgcg acaggctgaa gcgattgtct gaccggtgac aggctcatta
      241 acctgccctt aggactcggg agctgcgcaa ctcatgtatg aaaccctgct ggcgctgctg
      301 gtcgcgcacc tggtcgcgga cttccccctg cagccggact ggatgatccg ccgcaagcgg
      361 catcccgcgg tgctcggcct gcacgggctc atcgtcgtcg gcaccgccgc gttcgcgctc
      421 ggcggctggc cgagtacgct gctgctgatc ctcgtcggca cgcacctcgc catggatgcg
      481 gtcaaggttt acctgttgaa ggactcgctg cgcagcttca ccgtggatca ggtcgtgcac
      541 cttctggtga tcctcggcct ggcgctcggc tttccgggca cgtacgcgca gggctggtgg
      601 gcggcgctcc ccggaaactt cgccgcggcc tatgcgatcg gcctttgtct actcggcggc
      661 gtgatcgcga gcctcatggt cggggcgatc gtcatccgca aggccaccat gcctttcacc
      721 gggcagctga gcggggacat ctcggggctc gagagcggcg gcacgtacat cggctggctg
      781 gaacgcgcgc tggtgatgct gctgattctg atcggtcagc cggcgggagt cggcttcctg
      841 atcaccgcga agtcgatcct gcgctttggt gacgtgcgcg actcggcgca gcgcaagctc
      901 accgagtaca tcatcatcgg gactttcatg agcttcggct ggggactgct gatcgccgtg
      961 ctgacccagc tggcgatgcg gcactggatg gcaccctagg ggatgagttt gcgcagcgcc
     1021 ggggtcgaga gatcgtcgag gtggcgaaag gccggcgcga tcttctcgat ctgatcggcg
     1081 cccacctggt actcctcgaa gtagaccttc cattcgcgca cgatccgcca gatcctggcg
     1141 atgctctcgg cggcggcctc cgcagacagg gtgaacatct cgcggccggc aaaggcgttg
     1201 tcgagcgttg cgatccgtcc ctccggcccc acgccgaggt gcagccgccg ttcgggcgag
     1261 agactcgccc gcggcaggac atcgtagagc ggactcaggc gccacccggg aaggcgcggg
     1321 tcccagacga agccgtggtt gcgcagatga tcgtcgtcgt tgttgacgag gatgttgaag
     1381 atgaggcgct cgaagagctc gcgattgttc tcgcgaatca cgcgcgggtg gcagtagcgg
     1441 cgtaccgcct gggccagatc gccgtaggac ttgtccggtg agtccatctc atcgcacgcg
     1501 aggagcgtga gcgcactgac gaaccccagc cgcttctccg ccaagccgta cgcgggagcc
     1561 gtttcgagga actcctgcgc gagcggcgca tcctggcccg ccttggccca gtagcggtcg
     1621 aagcggcgga tcagcatcac ggtccgggac ccgagctgta tgagcctcac ctgcggcacg
     1681 ctgagacctg cggcggcggc cagccgcagc gtcgcggtct ccacgacggg aaccgccagc
     1741 gcatccttgc ggctcgggaa tttggcgagc cacagcaccc gctcgtcatc gcgcaccgtg
     1801 gctttggggc gggcgccgcc gagcgcgctc ccctcggcga agatctcctc gaggggggaa
     1861 ggaacgggca ggccctcgtc gatgcgctgc gcggcgtcca tcaggtactc gaggttgttc
     1921 caggtgccaa agcccggtgt cgcggacgag tcccgggacg agcggatgtc gagcgcgccc
     1981 acgcgctggc tgcccgcgtg caccaggtac gtcgactccg gcaggctgtt ggcgggtacc
     2041 ttgaggcggg actcgatgac gcgccggccc caggcatcgg gggctgcatc gcgaatgccg
     2101 ccgaacgagg gcagcttgtt gggtgggaag agagccttgc cgcggaccgc gagcttgtcg
     2161 cgcagggaga ggctcacggg atccacctcg agagcgccgg gacgcagcag gtagcgcagc
     2221 ccgtacgcga agctcgacga gaggagccgg gcaccctcct cgctcagggt gagctgccca
     2281 cacggcagcc actccgtatc gatataggcg aagacgaaca gcttctgctc gctcgcgctc
     2341 atgactttag aaatcgagct cgctgagctc gcgcttggac agatcgcgcg cgcggcgccg
     2401 ctgctccttc gcgctgagcg ccgccaacgc cggatccgtt tcggacagca gctcgaacag
     2461 cgtcttgccc ggcacgacgg catcgagata gcgcaggatc tgacccatcg cgatgccggg
     2521 atcgcccttc tccatccgat acgcggtgtt tcgggacaga ccggcgcgca gtgccgcctg
     2581 gtcctgtttg acgccgcgcg cgatgcgcag ccgtgcgaga gccgccccca gcttctttgc
     2641 ctcggcggcc tggctcgccg acagcaccgc ggactgagtg atcttcatgc tcgtttaatc
     2701 gtcctttgac taattaacac tcgattaatc gatcatatgc tagccgatgc ggcaagaaac
     2761 ggcaagctat aaatgcctct gaaatcatgc tttatagcgc taatgatcga ttaatcgatc
     2821 gcatgtctcg tgcccttgag atggccggcg agcggatcga ttccctgggc ggctagcata
     2881 ccaaggcatg tccgcgctcc tcgtcccgcc actctctctt tacgtgcact tcccgtggtg
     2941 cgtgcgcaaa tgcccgtact gcgatttcaa ttcctacgcc ctgcaggggg agttggccgc
     3001 cgagccctat gtcgcgcggc tcgcgcgcga tctcgaggcg caagcgcccg aagtcgccgg
     3061 ccggccggtg gagagcgtgt tcttcggcgg gggcaccccg agcctcttct ctcccgagtc
     3121 gatcgcccgc gtgctcggcg cggcgcggcg ccatctccat ctcagcgccg acgccgaggt
     3181 gacgctcgaa gcgaatccgg gggcgatcga gcgcggcgcg ttcggcgagt accgcgacgc
     3241 tggggtcacg cgcgtgtcgc tcggcgcaca gagcttcgat ccggctgcgc tcggagcgct
     3301 cgggcgcatt cactcgcccg ccgagacgcg ccgcgcggcc gaggagctgc acgccgcgtc
     3361 cctcacgaat ttcaatctcg acctcatgta cgccctgccc ggacaggagc gctcgggcgc
     3421 gcggagcgac gtggaggcgg cgctcgcgct cgcgcccgcg cacctctctc actaccagct
     3481 gacactcgag cccggcacgg tattcgcagc gcatccgccg ccgctcccgg acgaggacga
     3541 ggccgcggcg atgcaggagg agtgcgccac gctcctcgcg gccgcgggct tcggccacta
     3601 cgagatatcg gcctgggcgc gcccgggaag gcagtgccgg cacaacctca actac
//
LOCUS       DATLKL010000264         1770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_172135, whole genome shotgun sequence.
ACCESSION   DATLKL010000264 DATLKL010000000
VERSION     DATLKL010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172135"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VM713_03440"
     CDS             complement(<1..402)
                     /locus_tag="VM713_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83351.1"
                     /translation="MPGTLKLWRNAQLATCDAALGVIGRGAMVTRGAGIEWVGAETAL
                     PASLIASAAGGGAPEEHDLGGAWVTPGLIDCHTHLVFAGNRAGEYAERLRGKSYEEIA
                     RAGGGILSSMRAVRAASEQQLIDESAPRLAAL"
     gene            complement(410..>1770)
                     /gene="hutU"
                     /locus_tag="VM713_03445"
     CDS             complement(410..>1770)
                     /gene="hutU"
                     /locus_tag="VM713_03445"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HVH83352.1"
                     /translation="LVARWADWEHFHELDRKGLMMFGQMTAGSWIYIGSQGIVQGTYE
                     TFVETGRQHFGGSLTGKWILTAGLGGMGGAQPLAATLAGASMLAIECRPERIAKRLET
                     RYLDTQAASLEEALERLERARRAGTPLSVGLLGNACEVLPELLRRGVRPDAVTDQTSA
                     HDPVNGYLPQGWSVEKWDRLRAADPAQVTRESRPSMRRHVEAMLAFQKQGIPVFDYGN
                     NLRQVALDEGLKEAFAFPGFVPAYIRPLFCRGMGPFRWAALSGDPQDIYRTDQRVREL
                     LPADRHLHHWLDMARERIQFQGLPARICWVGLGDRHRLGLAFNEMVARGELKAPIVIG
                     RDHLDSGSVASPNRETEAMRDGSDAVADWPLLNALLNTASGATWVSIHHGGGVGMGYS
                     QHAGVVIVCDGTPAAARRIERVLWNDPATGVMRHADAGYEEAIACARERGLNLPFLPS
                     PH"
BASE COUNT          264 a          630 c          625 g          251 t
ORIGIN      
        1 gagagcggcg aggcgcggcg cgctctcgtc gatgagttgc tgctcgctcg cggcgcgcac
       61 cgcacgcatg gaggagagaa tgccaccgcc ggcgcgcgcg atctcctcat agctcttgcc
      121 gcgcagccgc tcggcgtact cgccggcgcg gttgccggcg aagacgagat gggtgtggca
      181 gtcgatgagt ccgggtgtca cccaggcgcc gccgagatcg tgttcctccg gcgccccgcc
      241 gcccgcagcc gaagcgatga gcgaagccgg cagggcggtc tccgcgccca cccattcaat
      301 accggcgccg cgcgtcacca tggcgccgcg cccgatgacg ccgagcgcgg catcgcaggt
      361 tgcgagctgc gcgttgcgcc agagcttcag cgtcccgggc acggagacgt cagtgcgggc
      421 tcgggaggaa cggcagattc agtccccgct cccgtgcgca ggcgatcgcc tcctcgtaac
      481 cggcgtccgc gtggcgcatg acgccggtcg ccggatcgtt ccacagcaca cgttcgatgc
      541 ggcgcgccgc ggcgggcgta ccgtcgcaga cgatgacgac cccggcgtgc tgcgagtagc
      601 ccatgccgac gccgccaccg tgatggatcg acacccaggt cgcgccgctc gcggtattga
      661 ggagcgcgtt gagcagcggc cagtcggcca ccgcatccga gccatcgcgc atcgcctcgg
      721 tctcccggtt ggggctcgcg accgagccgg agtcgaggtg atcgcgcccg atgacgatcg
      781 gtgccttgag ctccccgcgc gccaccatct cgttgaaggc gagcccgagc cgatgccggt
      841 cgccgaggcc cacccagcag atgcgcgccg gcagaccctg aaactgaatc cgctcgcgcg
      901 ccatatcgag ccagtgatgc aggtgccggt ccgcgggcag cagctcacgc acccgctgat
      961 cggtgcggta gatgtcctgc gggtcgccgg agagcgctgc ccagcggaac ggccccatgc
     1021 cgcggcagaa gagcggccgg atgtacgccg ggacgaaacc cgggaaggca aatgcctcct
     1081 tcaggccctc gtcgagggcg acctgacgca ggttgttgcc gtagtcgaag accgggatgc
     1141 cttgcttctg gaaggcgagc atggcctcga cgtggcggcg catcgagggg cgcgactcgc
     1201 gcgttacctg cgccggatcc gcggcgcgca ggcggtccca cttctcgacc gaccaccctt
     1261 gcgggaggta gccgttgacc gggtcatggg ccgaggtctg atccgtgacc gcatccgggc
     1321 gcacgccgcg gcgcagcagc tccggcaaca cctcgcacgc gttgccgagc aacccgaccg
     1381 acaggggcgt gcccgcgcgg cgcgcgcgct cgagccgctc gagcgcctcc tcgagactgg
     1441 ccgcctgtgt gtcgagatag cgtgtctcga gacgcttggc gatgcgctcc gggcggcact
     1501 cgatcgcgag catcgaggct cccgcgagcg tggcggcgag cggttgcgcc ccgcccatac
     1561 cgccgaggcc ggcggtgagg atccacttcc cggtgagact gccgccgaag tgctgccggc
     1621 ccgtctcgac gaaggtctcg taggtcccct ggacgatccc ctggctgccg atgtagatcc
     1681 aggagcccgc ggtcatctgg ccgaacatca tgagcccctt gcggtcgagc tcgtggaagt
     1741 gctcccagtc ggcccagcgc gcgacgagat
//
LOCUS       DATLKL010000265         1601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_36879, whole genome shotgun sequence.
ACCESSION   DATLKL010000265 DATLKL010000000
VERSION     DATLKL010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36879"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VM713_03450"
     CDS             complement(<1..294)
                     /locus_tag="VM713_03450"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HVH83353.1"
                     /translation="MTIVETSSPAAEPSAALIAEAEQLCQRMALVRERIGQVIFGQRK
                     VIDQTLITLLAGGHALLVGVPGLAKTRLVETLGIVLGLDVKRIQCTPDLMPADI"
     gene            594..1217
                     /locus_tag="VM713_03455"
     CDS             594..1217
                     /locus_tag="VM713_03455"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HVH83354.1"
                     /translation="MTRDFIIARLRAANGAMQQIRRGVLIGRRGDHDLNSEDMRHPGP
                     LVPAAVLVPLVARPAGMTVLLTQRTDNLADHAGQISFPGGHIEPADKGPEEAALREAF
                     EEIGLPFDRVEPIGRLDDYETRTGFLIVPIVGIVTPPFPLKLDPAEVADAFEVPLSFI
                     LDPRNHRRESRMFKGRERQFYVLPYAGRYIWGATASMLVNLHDVLTG"
     gene            1217..1498
                     /locus_tag="VM713_03460"
     CDS             1217..1498
                     /locus_tag="VM713_03460"
                     /inference="COORDINATES: protein motif:HMM:NF040327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HVH83355.1"
                     /translation="MARILFEFLLPIVLPTALYAAWLAWHRRRAIVTGAARVPAWQEG
                     PWFWCILAGVGLALIVFVVTAVLWGHAPGAKYQPPRIHNGEVIPGQFQD"
BASE COUNT          263 a          520 c          527 g          291 t
ORIGIN      
        1 gatgtcggcg ggcatgagat cgggcgtgca ctggatgcgc ttgacgtcga gcccgagcac
       61 gatgccgagc gtctcgacaa ggcgcgtctt ggcaagaccc ggcacgccca cgagcagcgc
      121 gtgaccgccc gcgagcaacg tgataagggt ttgatcgatg accttccgtt ggccgaatat
      181 gacttggccg atgcgctcgc gcaccagcgc catgcgttgg cagagttgct ccgcttcggc
      241 gatcagcgcg gcactcggct cggctgcggg cgaactggtc tccacaatgg tcacaggctc
      301 gactccctag cgggttccat ccggatatcc cgcgattaag gcatgaccat ggcgaaagga
      361 tcaacggata tcacgtcgcc cgcaagtgaa ttaaggccgg aggatcggtc cgggcaagtc
      421 gtgcggcccg agcgtacttg caacattctc ggtatccgga tccttcgctg cggcgattgc
      481 tgtctcgcgg acgtcgtcgg gcgcggcatc gaagtgccgc tcaacggcgt ccgacgcgtc
      541 agggcgcgaa gtcgctgcga gctcttcccc atacgtgcat tggagaggcc ggcgtgacgc
      601 gcgatttcat catcgcgcgc ctgcgcgcgg cgaacggcgc gatgcagcag atccgtcgcg
      661 gcgtgctgat tggccggcgc ggagaccatg atctcaatag cgaagacatg cgccaccccg
      721 gtccactcgt gccggcagca gtgctggtcc cgctggtcgc gcggcctgct ggcatgaccg
      781 tgctgctgac acaacggacc gacaatttgg ccgatcatgc gggccagatc agcttccccg
      841 gcggccacat cgagccggcg gacaagggcc cggaggaggc ggcgctgcgc gaggccttcg
      901 aggaaatagg gctgccgttc gaccgcgtcg agcccatcgg acgactcgac gattacgaga
      961 ctcgcaccgg ctttctcatc gtcccgatcg tggggatcgt gacgccgcca ttcccgctca
     1021 agctcgatcc cgccgaagtc gccgacgcgt tcgaggtgcc gctatccttc atcctcgatc
     1081 cgcggaacca ccgacgcgag agccgcatgt tcaagggacg cgaacgacaa ttctacgtat
     1141 tgccctatgc aggccgttac atctggggcg ctaccgcgag catgctggtc aatctccatg
     1201 atgtgctgac gggctgatgg cgcgcatcct gttcgaattc ctgctgccga ttgtcctgcc
     1261 gacggcgctc tatgcggcct ggttggcctg gcaccgcagg cgcgcgatcg tcactggcgc
     1321 cgcgcgcgtg ccggcctggc aggaggggcc gtggttttgg tgcattctcg caggagttgg
     1381 ccttgcgctg atcgttttcg tcgtgaccgc ggttctctgg ggccatgccc ccggcgccaa
     1441 ataccagcct cctcgcatac acaatggcga ggtgattccc ggccagttcc aggattagcg
     1501 ccatgacagc ggcgtcgctt ccgctccagc cctggatgac ggcgccgggg acccgccggg
     1561 tggtggcggc actcaccgcc aaggggtccg aggtgcgctt c
//
LOCUS       DATLKL010000266         4263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_183680, whole genome shotgun sequence.
ACCESSION   DATLKL010000266 DATLKL010000000
VERSION     DATLKL010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183680"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="VM713_03465"
     CDS             <1..1785
                     /locus_tag="VM713_03465"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HVH83356.1"
                     /translation="VTIVGSSFDPAAANDTVSFNSAPAAVLSASPTVLSVRVPAQAGT
                     APVALSTPANTAQSPRAFNADVTSPAAVAGVQVAVGSGPQALAFSPDGRKLYVASHGS
                     VTMLSAVTNQVIVPSPAHPNTAAAVGSGIVASPDGRRVYVAAGAAGVAAMDAALLEPI
                     AAESISGFTAGSSGPFNAQSLAVSPDGTMLYVADDLDGGVVRLITLAGTTYVSSPPFG
                     TGLVPVAVAASPDGSKLFAAIVDPTATTADFIAVLDPRSGALRSSIILGNGAMPAAIA
                     FSPDGKTAYVANRGANSVSLIDTASDTTGTSIPGLSSTTAVAVSPDGVKVLIANSGNG
                     TVTVIDVPSGAAATIPIPGALGPSLAGILVSPDGSHAYVADPAANNITEIGDSVTLTI
                     GIAGTGLGSVTSSPAGIACGTACQVRFPRGTSVALSALAGTGSSFAGWSGTGCGSGMI
                     TVQAPLSICTANFTNVSASTGAGGFAGCFIATAAFGSPMAKEVIVLRQFRDQHLVTNA
                     AGRAFVRFYYRYSPPIARFISGHEQLRAAVRAGLWPLVYSVKYPVTSAAALVLLLALV
                     ISRPRVTRRLAHVRSASLPRHPGSAGGR"
     gene            complement(1736..4231)
                     /locus_tag="VM713_03470"
     CDS             complement(1736..4231)
                     /locus_tag="VM713_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVH83357.1"
                     /translation="MLPRSLRACVLAALLCAPVALAAVPPPAAPLLSAAIAPQPLGAA
                     LKTYQDLTGLQGAYVADIVAGAHSRGARAGLVPAEALTRLLRGTGLTFEFLNERTFTV
                     SARRRTATPPAPPPTSDNPAQQIIITAARGGEPLSRVPVDAFVWSADALQASGVKTVA
                     DLSALTPVVAFDFFSSVGSGVYTDLAIRGIIDRHGSATGIFFDDIALPAARSNTFGRA
                     LPFYFDIKSIEVWRGPQGTLLGADTQGGAVKFVPNDPDLTQASGLARTEWAVTARGGP
                     SYEAGAAAGGPLLPEQLGFRLSAWYRADGGYVDRVDPFTGSVVDASSNRVSSRSARAA
                     LTWAPTEALSITPSLSYVSARAHDSPSFFTYPSDPSAGVLDNGSLVPQPFDDTFHLAS
                     VKVSASGAAAAFTSLTAYYHRAGNLTVDDTESVKWGGWGNPLGPAFPSSYANAVVTYT
                     RLRQDLFSQELRLRSAHSDSPLTWLAGLAYSNTRDTESYRVVGQYIPVLAGPLDSSNS
                     TTTAQHQLAGYGLVERLLLRHFTLSAGLRLEYDEYDSSSLAAPVFQGTEHHTLVVPRL
                     SLSYESGGHDVGYVSAARGYSPAGVDAALPTCFENPMPYPADTVWSYELGWKIGLFEG
                     AARVNATVFEARWNNGPAVTGNCLVTHVPGGAASSGFELAADATLSESRQLRAMLALS
                     YIDARYTDTVRSGGVLIVNRGDALGTPPVVTSPWNLTAALERDLLLGERTRLAVRAED
                     AFHSRNPGPFYTGISGSPFFAPGLGSDPSTNLVNLRASARLDRLEIATFLDNVFDAQP
                     TLLKRNKGVDVSNLYYATTFRPRTLGVAASWRF"
BASE COUNT          640 a         1454 c         1520 g          649 t
ORIGIN      
        1 gtcaccatcg tcggcagctc cttcgatccg gcggccgcca acgataccgt gtcattcaac
       61 tcggccccgg ccgcggtgct ctcagccagc cccacggtgc tctcggtgcg cgtccccgcg
      121 caggcgggca cggcgccggt cgccctcagc accccggcga acacggcgca gagtcctcga
      181 gccttcaacg ccgacgtgac atctccggcc gcggtggccg gcgttcaagt cgccgtcgga
      241 tccggaccgc aggccctcgc cttcagcccg gatgggcgca agctctatgt cgcgagccac
      301 ggatcagtga cgatgctgag cgccgtcacc aaccaggtga tcgtgccaag tccagctcat
      361 cccaacaccg cggcggctgt cggctccggc atcgtggcca gccccgacgg gcgacgggtc
      421 tacgttgccg ccggcgccgc aggcgtcgcg gccatggacg ccgcgctgct tgagccgatc
      481 gccgcggaat cgatctcggg atttaccgcc ggctcgagcg gcccgttcaa tgcgcagtcg
      541 ctcgccgtga gcccggacgg gacgatgctg tacgtcgccg acgatctcga cggcggcgtc
      601 gtgcgcctga tcaccctggc cggcacgacg tacgtgtcct cgcccccctt cgggacgggc
      661 ctggtcccgg tggccgtcgc cgccagcccc gatggcagca aactgtttgc ggcgatcgtc
      721 gatccgaccg cgaccaccgc cgacttcatc gcggtgctgg atcctcgcag cggcgcgctg
      781 cgctcatcga tcatcctggg gaacggcgcg atgccggcgg ccatcgcctt ctcgcccgac
      841 ggcaagacgg cttacgtggc gaaccgcggc gccaacagcg tgagcctcat cgacaccgcg
      901 agcgacacga ccggcacatc cattccgggg cttagctcca cgaccgccgt ggccgtcagt
      961 ccggacgggg tgaaggtgct gatcgccaac agtggcaacg gcaccgtcac ggtgatcgac
     1021 gtgccgagcg gcgcggccgc gaccattccc atacccggtg cattgggccc gtcgctcgcc
     1081 ggcatcctgg tgagtcccga cggcagtcat gcttacgtcg ccgatccggc ggcaaacaac
     1141 atcaccgaga tcggcgactc ggtgactctg accatcggaa tcgccggaac cgggcttggc
     1201 tcggtcacat cgagcccggc gggcatcgcc tgcggcacgg cctgccaggt ccgcttccca
     1261 cgggggacct cggtggcact gagcgccctg gcgggcaccg gctcgagctt cgcaggctgg
     1321 agcggcacgg gttgcggcag cggcatgatt acggtgcagg ctccgctgag catctgcacg
     1381 gcgaacttca ccaatgtctc cgcctcaacc ggcgcgggcg gcttcgccgg ttgcttcatc
     1441 gcgacggcgg ccttcggcag ccccatggcc aaggaggtga tcgtgctgcg ccagttccgc
     1501 gatcagcacc tggtgaccaa cgccgccggg cgtgccttcg tacggttcta ctaccgctac
     1561 tccccgccga tcgcgcggtt catcagtggg cacgagcagc tccgggcagc ggtgcgcgcc
     1621 ggcctgtggc cacttgtcta ctccgtcaag taccccgtaa cgtccgcggc tgcgctggtg
     1681 ctgctgctcg cgctcgtgat ctcgcggccg cgcgtcacgc gcaggctcgc gcacgtcaga
     1741 agcgccagct tgccgcgaca cccagggtcc gcgggcggaa ggtagtggcg tagtacaggt
     1801 tgctcacgtc tacgcccttg ttgcgcttga gcagtgtcgg ctgggcgtcg aagacattgt
     1861 cgagaaacgt cgcgatctcg agcctgtcga ggcgcgcgct cgcgcgcaga ttgaccaggt
     1921 tggtggaggg gtccgagccg aggccgggcg cgaagaacgg cgacccggag ataccggtgt
     1981 agaacggccc agggttgcgg ctgtggaagg cgtcttcggc gcgcaccgcg aggcgggtcc
     2041 gctccccgag cagcaggtcg cgctcgagcg cggcggtgag gttccagggc gaggtcacga
     2101 cggggggcgt gccgagcgcg tctccgcggt tgacgatgag cacaccgccc gagcgcaccg
     2161 tgtcggtgta gcgggcgtcg atgtaggaaa gcgcgagcat cgcccgcagc tgccgactct
     2221 cgctcagggt cgcatcggcg gccagctcga agccactgct ggccgcgccg cccgggacgt
     2281 gagtcaccag gcaattgccg gtcacggccg ggccgttgtt ccagcgcgcc tcgaacaccg
     2341 tggcgttcac ccgtgcggcg ccctcgaaca atccgatctt ccatccgagc tcgtagctcc
     2401 acaccgtgtc ggccggatag ggcatcgggt tctcgaagca ggtcgggagc gccgcgtcga
     2461 cgccggcggg cgagtagccc cgggcggcag agacgtaccc cacgtcgtgt ccgccggact
     2521 cgtaggagag cgagagccgc ggtaccacca gcgtgtggtg ctcggtgccc tggaagaccg
     2581 gggccgcgag ggagcttgag tcgtactcgt cgtactcgag gcgcagcccg gcgctcagcg
     2641 tgaaatgccg cagcagcaga cgctccacca gcccgtagcc ggccagctgg tgctgcgcag
     2701 tcgtcgtgga gttgctggag tcgagcggtc cggccagcac cgggatgtac tgcccgacca
     2761 cgcggtaaga ctcggtgtcg cgggtgttgg agtaggcgag tcccgcgagc cacgtgagcg
     2821 ggctgtccga gtgcgccgat ctcagccgca gctcctgtga gaaaaggtcc tgccgcagcc
     2881 gcgtgtaggt gacgaccgca ttggcgtagc tcgagggaaa ggccggtccg agaggattgc
     2941 cccagccgcc ccatttgacc gactcggtgt cgtcgaccgt cagattgccg gcgcggtggt
     3001 agtaggcggt gagactggtg aaagccgcgg ccgcaccgct cgcgctgacc ttgactgatg
     3061 ccagatggaa cgtgtcgtcg aacggttgtg gcaccagact gccgttgtcg agcactccgg
     3121 cgctgggatc ggaggggtag gtgaagaagg acggcgagtc gtgcgcccgc gcagagacat
     3181 agcttaggga cggcgtgatg ctgagcgctt ccgtcggcgc ccaggtgagg gcggcgcggg
     3241 cgctgcggct gctgacgcgg ttcgagctcg catcgacgac gcttccggtg aacggatcga
     3301 cccgatcgac gtaaccgccg tcggcgcgat accaggcact caggcggaat ccgagctgct
     3361 cggggagcag cgggcccccg gcggccgcgc cggcctcgta gctcgggccg ccgcgggccg
     3421 tcacggccca ttcggtgcgg gcgagccccg aggcctgtgt caggtcgggg tcgttcggga
     3481 cgaacttgac cgctccgccc tgggtgtcgg caccgagcag cgtcccctgc gggccgcgcc
     3541 agacttcgat gctcttgatg tcgaaataga agggcagtgc gcgtccgaaa gtgttgctgc
     3601 gtgcggccgg cagcgcgatg tcgtcgaaga agatgccggt ggcgctgccg tgccgatcga
     3661 tgatcccgcg aatcgcgagg tcggtgtaga cgccgctgcc cacgctcgag aagaaatcga
     3721 acgcgacaac gggcgtgagg gcgctcaggt ccgcgaccgt cttgactccc gacgcctgca
     3781 gggcgtcggc gctccagacg aatgcatcga ccggcacgcg cgagagcggc tcgccgccgc
     3841 gcgcggcggt gatgatgatc tgttgcgccg ggttgtcact ggtcggcggc ggtgcgggcg
     3901 gcgtcgctgt ccggcgtcgg gcggagacgg tgaaggtacg ctcgttgaga aactcgaacg
     3961 tgagaccagt gccgcgcagc agccgcgtca gcgcctcggc gggcacaagt cccgcgcgcg
     4021 cgcccctgct gtgcgcgccg gccacgatgt cggcgacata ggcaccctgc agccccgtca
     4081 ggtcctggta cgtcttgagg gccgcgccga gcggctgggg cgcgatcgca gcgctcagta
     4141 gcggcgcggc gggcggcggc acggccgcga gcgccaccgg cgcgcacagt agcgccgcca
     4201 gcacgcacgc acgcaggctg cgcggcagca cggactactt cttgaatacc cggatggcgg
     4261 tcg
//
LOCUS       DATLKL010000267         1970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_3007, whole genome shotgun sequence.
ACCESSION   DATLKL010000267 DATLKL010000000
VERSION     DATLKL010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3007"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VM713_03475"
     CDS             <1..268
                     /locus_tag="VM713_03475"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HVH83358.1"
                     /translation="DVTRRLRTLAVGDQEYVPIYGGEAVLHGGEVVGRLRSCAYGFTV
                     QRNLAYSYLPVELKPGAEVEVEVFGERVPATVMRDRVLEPQPTR"
     gene            285..1154
                     /locus_tag="VM713_03480"
     CDS             285..1154
                     /locus_tag="VM713_03480"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HVH83359.1"
                     /translation="MALWRGLQVAISPLSGGLTNENYLVEAGGQRYVMRLPGQSTELL
                     SIDRENEVYNTKAAASTGIGPKVLEHVAGSDVMVLEFIPGPTMSAQTLRSERMAARMA
                     QSFHRLHAAPRFLKDFDMFRLIEYYLGIVQEHEVTIPDGYRDWLPTVATIERAVGVAA
                     LPSAPCHNDLLCENFIDDGAALRIVDYELSGNNDPCFDLGNTAQEAEFDQDLRAALCQ
                     AYFGTNDRRQLARMNLFALMSDVGWTLWGAIQARISAVDYDFKGYYTGRWERALEVLR
                     SDRMQRWMAEAAN"
     gene            1278..1937
                     /locus_tag="VM713_03485"
     CDS             1278..1937
                     /locus_tag="VM713_03485"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVH83360.1"
                     /translation="MAVAEPVAARSQSESAYLRIRQRIVSLEMPPGSVVNEARLREEL
                     KIGRTPIREALQRLARENLVKSVPHRGTFVTDVNITDLARITEVRVVLEAHAARMAAE
                     KLTNTDRTGIQELLEALRRTRITDQRDLMQLDQRIHRAIYRAARNPFLEATLERYFNL
                     SLRLWYLVLDREVPLREAVDEHVELLRAILAGDGELAETIMRRHVTNFEHEIRKVLVE
                     G"
BASE COUNT          352 a          637 c          668 g          313 t
ORIGIN      
        1 cgatgtgacg cgccggctgc gaacgctcgc ggttggcgac caggaatatg tccccatcta
       61 cggcggcgag gctgtgctgc acggaggcga ggtcgtcggg cggctgcgca gctgtgcgta
      121 tggattcaca gtccagcgca acctcgccta ttcctatctg ccggtcgagc tgaagccggg
      181 agcggaggtc gaggtcgagg tcttcggcga gagggtgccc gccaccgtca tgagggaccg
      241 agtgcttgag ccgcagccca cgcgctgacg aggcggcggc gagcgtggcc ttgtggaggg
      301 gactccaagt cgcgatctct ccgctgtccg gaggcctgac caacgagaac tacctggtcg
      361 aggccggcgg ccagaggtac gtgatgcggt tgcccggcca atcgaccgag ctgctctcga
      421 tcgaccgcga gaacgaggtc tacaacacca aggcggccgc atcgaccggg atcggcccga
      481 aggtgctcga gcatgtggcc ggaagcgacg tgatggtgct ggagttcatc ccagggccga
      541 ccatgtcggc gcaaacgctg cgctcggagc gcatggccgc gcgcatggcg cagtcgttcc
      601 atcgtctgca cgccgcgccg cgattcctca aggacttcga catgttccgt ctcatcgagt
      661 actacctcgg catcgtccag gagcacgagg tcaccatccc ggacggctac cgggactggc
      721 tccccaccgt cgccacgatc gagcgcgcgg tcggcgtcgc ggcgctcccg agcgctccgt
      781 gccacaacga cctgctgtgc gagaacttca tcgacgacgg cgcggcgctg cgcatcgtcg
      841 actacgagct gagcggcaac aacgacccgt gcttcgacct gggcaacact gcgcaggaag
      901 ctgagttcga ccaggacctg cgggccgcgc tgtgccaggc gtacttcggc acgaatgacc
      961 ggcgtcagct cgcgcgtatg aacctctttg cgctcatgtc cgacgtcggt tggacgttgt
     1021 ggggcgccat ccaggccagg atctcggccg tcgactacga cttcaagggc tactacacgg
     1081 gacggtggga gcgggccctc gaggtcctgc gctcggaccg catgcagcgc tggatggcag
     1141 aagccgcgaa ttgacgtgac gccggaccgg tggtacgttc cgcgacggta tatcggtcgt
     1201 atatcgccgg tgatcagggg cccccgcgaa atcgaagatt tcgtggggtc atgttcctgt
     1261 agagggatgg ggaaggaatg gcggtcgccg agcccgtagc cgcgcggtcc cagagcgaaa
     1321 gcgcatacct gcgcatccgc caacggatcg tcagcctcga aatgccgccc ggttcggtcg
     1381 tcaacgaggc gaggctccgc gaggagctga agatcggccg gacgcccatc cgcgaagcgc
     1441 tgcagcgtct ggccagagaa aacctggtga agtccgtccc gcatcgcggg acgttcgtca
     1501 ccgacgtgaa catcacggac cttgcccgaa tcaccgaggt ccgtgtcgtg ctcgaggcgc
     1561 acgcggccag gatggcggcg gagaagctga cgaataccga ccggaccggg atccaggaac
     1621 tcctcgaggc gctgcggcgc acgcgcatca ccgaccagcg agacctgatg cagctcgacc
     1681 agcggatcca ccgcgcgatc tatcgcgccg cccgcaatcc gttcctcgag gcgaccctgg
     1741 agcgctactt caacctcagc ctgcgcctct ggtacctggt gctggaccga gaggtgccgt
     1801 tgcgcgaggc cgtggacgag cacgtcgagc tgctgcgcgc catcctggcg ggcgacggcg
     1861 agctggcaga gacgatcatg cgccgccacg tcacgaactt cgagcacgag atccgcaagg
     1921 ttctggtcga gggatagctg atgccgaaag tcttcttcgc caccgacctg
//
LOCUS       DATLKL010000268         2114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_48076, whole genome shotgun sequence.
ACCESSION   DATLKL010000268 DATLKL010000000
VERSION     DATLKL010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48076"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..562)
                     /locus_tag="VM713_03490"
     CDS             complement(149..562)
                     /locus_tag="VM713_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HVH83361.1"
                     /translation="MNLKGSKTEKNLKDAFAGESQANRRYLYFAQKADVEGFNDVSAV
                     FRSTAEGETGHAHGHLEYLEAVGDPATGLPIGETKLNLKAAIAGETHEYTDMYPGMAK
                     TARDENFDEIADWFETLAKAERSHANRFQKALDGI"
     gene            715..984
                     /gene="xseB"
                     /locus_tag="VM713_03495"
     CDS             715..984
                     /gene="xseB"
                     /locus_tag="VM713_03495"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007894317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HVH83362.1"
                     /translation="MARDSKAPDFEQALAELEGLVERLEHGDLPLEEALKAFERGVAL
                     TRQCQASLQAAQQKVEILLKRGARAQIEPFEESADETPAGPTAPE"
     gene            996..1910
                     /locus_tag="VM713_03500"
     CDS             996..1910
                     /locus_tag="VM713_03500"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007877.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HVH83363.1"
                     /translation="MKVARSPGADFAARVAACQARIERVLDEVLRLPDPGTERLRDAM
                     RYSTLGAGKRLRPTLVYLTGESFGAAPARLDAPAAAVELIHVYSLVHDDLPAMDDDDL
                     RRGRPSCHRAYDEGTAVLVGDALQALAFSVLAHDPMAGATPEVRLEMIRVLAHAIGTG
                     GMAGGQAVDLEAVGRTLTVEAVENVHRRKTGALIQASVLLGALAAGVSGAQELAALGH
                     FGAEIGLAFQIQDDILDVEGDPRLLGKSTGADAALAKPTYPSTAGLEAARARTGELRD
                     AAIAALEPLGGRAAGLAELARFVVSRAS"
BASE COUNT          294 a          736 c          748 g          336 t
ORIGIN      
        1 cgtagaactt cggatcccgc cactcgaggg gcaggcgcgt cggagcctcg aggctcccct
       61 cgcgcgcccc cggcggcggg ctgggcgtgc cgttcatgag atgcgcatcg ggcccgcttc
      121 agatcgcggg cccgacgcgc acgttccatc agattccgtc gagcgccttc tggaagcgat
      181 tggcgtgcga gcgctcggcc tttgcgagcg tctcgaacca gtcggcgatc tcgtcgaagt
      241 tctcgtcgcg cgcggtcttg gccatgcccg ggtacatgtc ggtgtactcg tgggtctcgc
      301 cggcgatcgc ggccttgagg ttcagcttcg tctctccgat cggcagcccc gtggcaggat
      361 cgcccaccgc ctcgaggtac tcgaggtggc cgtgggcgtg gccggtctcg ccctccgcgg
      421 tcgagcggaa cacggcggag acgtcgttga agccttcgac gtcggccttc tgggcgaagt
      481 acagatagcg gcgattggcc tgcgattcgc cggcaaacgc gtccttgaga ttcttttccg
      541 tcttgctgcc tttgagattc atggagctcc tcacttagac tcagtctaac gaagggctgc
      601 gactgtatcg ccctcccccg agtgacgtca agaacgcgcg ttgaccctcg gcgggggcgc
      661 tgtgtaacgt tgccggccct cggaacccga ccgaggcccc gaaggagacc cattttggcc
      721 cgcgatagca aagccccgga cttcgagcag gcgcttgcgg agctcgaggg gctggtcgag
      781 cggctcgagc acggcgatct gccgctcgag gaggccctga aggcgttcga gcgcggcgtg
      841 gcgctcaccc gccagtgcca ggcctcgctc caggcggcgc aacagaaggt cgagatcctc
      901 ctgaaacgcg gcgcccgggc gcagatcgag ccgttcgagg agtccgcgga cgagaccccc
      961 gccgggccca ccgcgcccga gtgacgagcg cgcaggtgaa agtcgcccgc tcccccggcg
     1021 ccgattttgc ggcgcgtgtc gcggcctgcc aggcgcgcat cgagcgcgtg ctcgatgagg
     1081 tgctgcggct accggatccc ggcaccgagc ggctgcgcga tgccatgcgc tacagcaccc
     1141 tcggcgccgg caagcggctg cggccgacac tcgtgtacct cacgggcgaa tccttcggcg
     1201 ccgcgcccgc gcggctcgat gccccggccg cagcggtcga gctgattcac gtctacagcc
     1261 tggtgcacga cgacctgcct gcgatggacg acgatgatct gcggcgcggc cgcccgagct
     1321 gccaccgggc ctacgacgaa gggaccgcgg tgctcgtggg cgatgcgctg caggcgctcg
     1381 ccttctcggt gctcgcgcac gacccgatgg ccggcgccac gcccgaggtg cggctcgaga
     1441 tgatccgcgt gctcgcgcat gccatcggca ccggcggcat ggccggcggg caggcggtgg
     1501 atctcgaggc cgtcggacgc acgctcacgg tcgaggccgt cgagaacgtg catcgccgca
     1561 agactggggc gctcattcag gcgagcgtgc tgctcggggc gctggctgcc ggggtgagcg
     1621 gcgcccagga gctcgccgcc ctcgggcatt tcggcgccga gatcgggctc gcgttccaga
     1681 tccaggacga tattctcgac gtcgagggcg acccgaggct tctcggcaag tcgaccggcg
     1741 ccgatgcggc gctcgcgaag cccacttatc cgagcaccgc cgggctcgag gccgcgcgcg
     1801 cgcgtacggg cgagctgcgc gatgcggcga tcgccgcgct cgagcccctg ggagggcgcg
     1861 ccgccgggct cgccgagctc gcccgattcg tcgtcagccg cgcgagttga gatgcgcgaa
     1921 gcgggcggta cactttaggc cacgatgagc gtgtccccgg acaaatatcc gctgctcttg
     1981 ggcatcgaga gccccgcgga cctgcgccgc ttggccccgg ctcagcttcc agcgctggcc
     2041 gcggagctgc gcgagtttct catccaaagt gtcagcacgc ggggcggtca tttcgccgcc
     2101 ggcctcggca cggt
//
LOCUS       DATLKL010000269         1541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_25543, whole genome shotgun sequence.
ACCESSION   DATLKL010000269 DATLKL010000000
VERSION     DATLKL010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25543"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VM713_03505"
     CDS             <1..426
                     /locus_tag="VM713_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVH83364.1"
                     /translation="QGHGDLSAAEIGERLGMSASTCWRRISRLSELGVIKRRVAVLER
                     EKVGLSVMVFSHVKLSGHGRDALLKFEQAVRAHPEILECYTLMGETDFLLRIVCRDIK
                     AYEAFFLDHLSRFPGVQSVNSSIALAVIKETTALPLHLG"
     gene            complement(389..733)
                     /locus_tag="VM713_03510"
     CDS             complement(389..733)
                     /locus_tag="VM713_03510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HVH83365.1"
                     /translation="MRFVAHAARTLELRNVTAVHSRAETLRPKVPFDTVTARALAPLP
                     ELLALLAPLCGPKTHVLAMKGRWPGEELERLPAPWRLAGSRALKVPGLDAERCVLNLS
                     ISPDAAAAPSSP"
     gene            738..1079
                     /locus_tag="VM713_03515"
     CDS             738..1079
                     /locus_tag="VM713_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83366.1"
                     /translation="MPLESISVNCRAGVHDGQRQPREAATRADIGDARAGEVRADREA
                     VEKVVRQHLLAALDGGEVVAAVPALELIEHPEQAGRVAGREADAEKRGIADQALGRAQ
                     RTGSVNDSGRG"
     gene            1098..>1541
                     /locus_tag="VM713_03520"
     CDS             1098..>1541
                     /locus_tag="VM713_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH83367.1"
                     /translation="MRLLLVEDDSMIGEAIRAGLKRDGFAVDWVRDTASAGRALEAEP
                     FELLLLDLGLPGGDGLGLLTALRARGSPLPVLIITARDAVADRVAGLDAGADDYLVKP
                     FDLDELAARIRALLRRKSGRTEPHLEHQGVVLDPATRRVTRDGADV"
BASE COUNT          214 a          539 c          556 g          232 t
ORIGIN      
        1 caggggcacg gggacctctc ggcggccgag atcggagagc ggctcgggat gagcgcgtcc
       61 acctgctggc ggcgcatcag ccgcctctcg gaactcggtg tcatcaagcg ccgcgtggcg
      121 gtgctcgagc gcgagaaggt cggcctctcg gtcatggtgt tctcgcacgt gaagctctcg
      181 ggccacgggc gcgacgcgct cctgaagttc gagcaggcgg tgcgcgcaca ccccgagatc
      241 ctcgagtgct acacgctgat gggcgagacg gacttcctgc tgcgcatcgt gtgccgcgac
      301 atcaaggcgt acgaggcatt cttcctcgac cacctgtcgc gctttccggg cgtgcagtcg
      361 gtcaactcct cgatcgcgct cgcggtcatc aaggagacga cggcgctgcc gctgcatctg
      421 ggctgatgga caaattcaat acgcaccgct ccgcgtccag tccggggact ttgagtgcgc
      481 gcgagccggc gaggcgccag ggtgcgggaa gcctctcgag ctcctccccc ggccagcgcc
      541 ccttcatggc gagcacgtgc gtcttgggtc cgcagagcgg cgccagcagc gcgagcagct
      601 ccggcagcgg ggcgagcgcg cgcgccgtga cggtgtcgaa cggcaccttc gggcgcagcg
      661 tctcggcgcg cgaatgcacc gccgtcacgt tgcgcagctc gagcgtgcgc gccgcgtgcg
      721 cgacgaaacg caccttcttg ccgctcgaat cgatcagcgt gaactgccgc gcgggggttc
      781 acgatggcca gcggcagccc cgggaagccg ccacccgtgc cgacatcggc gatgcgcgtg
      841 ccggcgaggt ccgggctgat cgcgaggctg tcgagaaggt ggtgcgtcag catctcctcg
      901 cggctctcga tggcggtgag gttgtagctg cggttccagc gctcgagctc atcgagcacc
      961 ccgagcaggc gggccgcgtc gccgggcgcg aggctgacgc cgagaagcgc ggcatcgcgg
     1021 atcaggcgct cggtcgggct caacgcacgg gctccgtgaa cgattcgggg cgcggctgat
     1081 catactcgcg gaaccgtatg cgactgctcc tcgtagaaga cgactccatg atcggcgagg
     1141 ccatccgcgc gggactgaag cgcgacgggt tcgccgtcga ttgggtgcgc gacaccgcct
     1201 ccgccggccg ggctctcgag gccgagccct tcgagctgct gctgctcgac ctggggctcc
     1261 cggggggtga tggcctcggg ctcctcacgg cgctgcgcgc ccgcggctcc ccgctcccgg
     1321 tcctcatcat cacggcgcgc gatgcggtgg cggaccgcgt ggcggggctc gatgccggcg
     1381 ccgacgacta cctcgtgaag cccttcgacc tcgacgagct cgcggcgcgc atccgcgcgt
     1441 tgctgcgccg caagtccggg cgcaccgagc cgcacctcga gcaccagggc gtggtgctcg
     1501 atccggccac gcgccgcgtg acccgcgacg gcgccgacgt g
//
LOCUS       DATLKL010000270         2780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_205946, whole genome shotgun sequence.
ACCESSION   DATLKL010000270 DATLKL010000000
VERSION     DATLKL010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205946"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..823)
                     /locus_tag="VM713_03525"
     CDS             complement(254..823)
                     /locus_tag="VM713_03525"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVH83368.1"
                     /translation="MAQSSGKKSSVNNGVNVQALLDAREALRNAPAAAEFTWRASCKW
                     KNGTHARSTVRGFRGLGEEQSHKTEFSFETDHPEVFASEDQGATPVELVLVGLASCLT
                     AGVAAVAQNRGIQLRSVEAKLEGKMNLYGILGIDSNVRNGYDDIKVTFHIDADASKQD
                     IEALVAQSQKRSAVYDIVTNPTNVTVEVV"
     gene            1034..2347
                     /locus_tag="VM713_03530"
     CDS             1034..2347
                     /locus_tag="VM713_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HVH83369.1"
                     /translation="MEASRRFGFETRQVHAGQRPDPNTGARAVPIFQTTSYVFEDPAS
                     AAAYFNLQEYGNTYTRIVNPTVAVFEERVASLEGGCGAVAFASGLAAQAAALFTLLTP
                     GDHVVSSAALYGGTVNQFKHLLRKLNVEVTWVDPDELGAWKAAVRGNTKAFFGETIGN
                     PGGNVLDIAAVAAIAHEHGLPLLVDNTFATPYLCRPLEWGADIVLHSATKYIGGHGTS
                     IGGVVVDSGQFNWSNGRFPVIAEPSPAYHGLQFHETFGTYGYLMKLRAETLRDLGGCL
                     SPFNAFLFLQGLETLSLRMARHVENAMTIARYLESHELASHVTYPGLRSSRYRALAEK
                     YLPQGAGSVFSFDIRGGREAGQDFIRGVTLWSHLANVGDAKSLVIHPASTTHRQHDER
                     ELVAAGVRPGTIRLSVGIEDADDPIWDLEQGFARARTAHSLEGTR"
     gene            2344..>2780
                     /locus_tag="VM713_03535"
     CDS             2344..>2780
                     /locus_tag="VM713_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HVH83370.1"
                     /translation="MSSFDLERFQDPLAIQRVLFSAKTIAIVGLSGNPLRASNFVGFY
                     LQRHGYRVIPVNPREAEILGERSFASLRELPVAVDIVNVFRAPAALPEIARDAVAIGA
                     KCLWCQFGVINEEGARIAEEGGLTVVMDRCIKVEHARYAGRMH"
BASE COUNT          436 a          928 c          903 g          513 t
ORIGIN      
        1 gcatcgtcat gaagccgccg ggatctggcc cctcgtaggg gtagcccggc agccggctct
       61 gccagtttgg cgtgagcaga cgcagcgact cccagcgttc acgccgccag gagttcgcca
      121 cttcgccccg ctcgagaact atgtgatcga tcgagtgctc ggtcagagcg cggctcgcgg
      181 cgagcccact gtgcccgcca ccgacgacga ccgtggtgat gtgttcgatg gcgagcccgt
      241 cgcggagttt ccgctacacg acctcgaccg ttacgttggt cggattggtg acgatgtcgt
      301 atacggccga gcgcttctgt gactgcgcca ccagcgcctc gatgtcctgc ttcgaagcgt
      361 cggcatcgat gtggaaggtc actttgatgt cgtcgtagcc gttgcgcacg ttgctgtcga
      421 tgccgaggat gccgtagaga ttcatctttc cctcgagctt cgcctccacg gagcgcagtt
      481 gtatgccgcg gttctgcgct acggcggcca caccggccgt cagacagctc gcgagtccga
      541 cgagcaccag ctcgaccggg gtcgcgccct ggtcctcgga ggcgaagacc tcgggatgat
      601 cggtctcgaa cgagaactcg gtcttgtgcg attgctcttc gccaagcccg cgaaagcccc
      661 gcaccgtcga gcgggcatgg gtgccgtttt tccacttgca ggatgcacgc caggtgaact
      721 ccgctgcggc cggtgcgttc ctgagcgctt cccttgcgtc gaggagcgcc tggacgttga
      781 caccattgtt gacgctggat ttcttgccgc tggactgggc catgttctgt ctcctggctt
      841 tccgggaacg cccccgatgc cctgctcgcg tggcaggact atcgaggcta cgcttgctac
      901 cggcagggta caagcgccga ggatctaaca ccatgaccta tcggttataa gctagtgcgc
      961 ggtggcaatt ggagccgccg cagcgcaagc ctatgatcgc gcgggtccct cgcgccattc
     1021 gggagttaag tccgtggaag cgtcacgtcg tttcggcttc gagacccgtc aggtgcacgc
     1081 cggccagcgt ccggacccca acaccggtgc gcgcgcggtg cccatttttc agaccacgag
     1141 ctacgtcttc gaggacccgg cctcggcggc cgcgtacttc aatctccagg agtacggcaa
     1201 cacctacacg aggatcgtga acccgaccgt ggccgtgttc gaggagcgcg tcgcgagtct
     1261 cgagggcggc tgtggagcgg ttgccttcgc gagcggactg gcggcgcagg cggcggcgct
     1321 cttcacgcta ctgacgccgg gcgatcatgt ggtgtcgtcg gcggccctat atggcggcac
     1381 cgtcaaccag ttcaagcatc tgctgcgcaa gctgaacgtg gaggtgacct gggtcgatcc
     1441 cgacgagctc ggcgcctgga aggccgcggt gcgcggcaac accaaggcct tcttcggcga
     1501 gacgatcggc aaccccggcg gcaacgtgct cgacatcgcg gccgtcgcgg ccatcgccca
     1561 cgagcacggg ctgccgctgc tcgtcgacaa caccttcgcg acgccctacc tctgccggcc
     1621 cctcgagtgg ggcgccgaca tcgtgctgca ctcggccacc aagtacatcg gtggccatgg
     1681 tacgagcatc ggcggagtgg tggtcgattc cgggcagttc aactggtcga acggccgctt
     1741 tcccgtgatc gcggagccct cgcccgccta tcacggtctg cagtttcacg agaccttcgg
     1801 cacctacggg tacctcatga agctgcgcgc cgagacgctg cgcgacctcg gcggctgcct
     1861 gagtcccttc aacgctttct tattcctcca ggggctcgag accttgtcgc tccgtatggc
     1921 gcgccacgtc gagaacgcca tgacgatcgc ccgctacctc gagtcgcacg agctggcctc
     1981 gcacgtgacg taccccggcc tgcgcagcag ccgttaccgc gccctcgcgg agaagtacct
     2041 gccgcagggc gcaggctcgg tgttctcttt cgatatccgt ggcggccgcg aggcgggaca
     2101 ggacttcatc cgcggcgtga cgctctggtc gcatcttgcg aacgtcggcg atgcgaagag
     2161 cctcgtcatt cacccggcga gcacgacaca tcgccagcac gacgagcgcg agctggtggc
     2221 ggcaggcgtt cgccccggaa cgatccggct atccgtcggc atcgaggatg cggacgatcc
     2281 gatctgggat ctcgagcagg gcttcgcccg cgcgcgtacc gcgcattccc tggagggcac
     2341 gcgatgagca gcttcgacct cgagcgcttt caggaccccc tggcgatcca gcgtgtgctc
     2401 ttcagcgcca agaccatcgc gatcgtcggg ctgtcgggta acccgctgcg tgccagcaac
     2461 ttcgtcggct tctacctgca gcggcacggc taccgcgtga ttcccgtgaa tccgcgcgag
     2521 gcggagatcc tcggcgagcg cagcttcgcg agcctgcgcg agctcccggt tgccgtcgat
     2581 atcgtcaacg tcttccgcgc gccggcggcg ctgcccgaga tcgcccggga tgccgttgcg
     2641 atcggcgcca agtgcctgtg gtgtcaattc ggcgtgatca acgaggaagg ggcgcgcatc
     2701 gcggaggaag gcggtctcac ggtcgtgatg gaccgttgca tcaaggtcga gcacgcccgc
     2761 tacgccggac gcatgcactg
//
LOCUS       DATLKL010000271         1585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_138538, whole genome shotgun sequence.
ACCESSION   DATLKL010000271 DATLKL010000000
VERSION     DATLKL010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138538"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VM713_03540"
     CDS             <1..201
                     /locus_tag="VM713_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVH83371.1"
                     /translation="QLGSIVMQRQLIREVWGPGREDDSRSLRVCIKNLRAKLEPDPRQ
                     PRYLITETGVGYRLRADDDAAA"
     gene            complement(467..913)
                     /locus_tag="VM713_03545"
     CDS             complement(467..913)
                     /locus_tag="VM713_03545"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HVH83372.1"
                     /translation="MLHEQELKTFVEGTTNFFEVAAQQPASIGSPYLLEGAPAVHEYS
                     GVINISGKREGVVIFTAPKAMLTVLLMKMQENDFSHETMRDLVGEVANTISGNARRDF
                     GRDFVISVPSVLSGEKPAIPQRPGLRSFIIPINWRSHSAKLVVSLT"
     gene            complement(913..1269)
                     /locus_tag="VM713_03550"
     CDS             complement(913..1269)
                     /locus_tag="VM713_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH83373.1"
                     /translation="MKLMIVDDSNIMRRRIERSQQFEDLELVGTAGNGVEAIELFKRT
                     DPDVVTMDLTMPRMDGIECISQLVALKPEVRILVVSALADKATAVEAMEKGANGFLNK
                     PFTDRQLNEAIAELMR"
     gene            complement(1298..>1585)
                     /locus_tag="VM713_03555"
     CDS             complement(1298..>1585)
                     /locus_tag="VM713_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH83374.1"
                     /translation="IAPDTLKAAAVRQQLVTEAEAALMDTRTAMALIFRPGFSTQDAV
                     SMDAGRGVGMDVVARSVYALGGKIGVSTHPGKYTRFKISLPAGHASSSAVA"
BASE COUNT          262 a          510 c          543 g          270 t
ORIGIN      
        1 cagctcggct cgatcgtcat gcagcgtcag ctgatccgcg aggtgtgggg gccggggcgc
       61 gaggatgact cacggtcgct gcgggtgtgc atcaagaacc tgcgggccaa gctcgagccc
      121 gacccgcggc agccgcgcta cctgatcacg gagacggggg tcggttaccg gctgcgcgcc
      181 gacgacgacg cggctgcgta acccaggtgc ggcgaaaagg acggcgggcg tgagtacccg
      241 ccgtcaagcg accgtgcggc acgagcccgg accgccggtc tcggaggttg acgctagcca
      301 tccgtcgacg ggagatcttt agggactcct taggaagttg tgatttagcg aggatcgagc
      361 tggaggcgcg acgagcagtc ggcaagtgca ggggccggac gcgcaggaac gcgtccgctc
      421 cgcaggcgcg cggtgattga ccaggtgtcg cgcggggcgc ggccggtcag gtcagggaga
      481 cgacgagctt cgccgagtgg ctgcgccagt tgatcgggat gatgaacgag cgcaggccgg
      541 gccgctgcgg aatcgcgggc ttctcacccg acagcaccga gggcaccgag atcacaaagt
      601 cgcggccgaa gtcgcggcgc gcgttgcccg agatggtgtt ggccacctcg cccacgagat
      661 cgcgcatggt ctcgtggctg aagtcgttct cctgcatctt catcagcagc acggtcagca
      721 tggccttggg cgcggtgaag atcaccaccc cctcgcgctt gcccgagatg ttgatgacgc
      781 ccgagtactc gtgcaccgcc ggcgcgcctt ccagaagata cggcgagccg atcgaggccg
      841 gctgctgggc ggctacctcg aagaagttgg tcgtcccctc gacgaaagtc ttgagctcct
      901 gttcgtgaag cattagcgca tcagctccgc gatcgcttcg ttcagctgcc gatcggtaaa
      961 cggcttgttg aggaagccat tcgcgccttt ttccatcgcc tctaccgccg tcgccttgtc
     1021 ggcgagggcc gaaaccacca ggatccgcac ctcgggcttg agcgctacca gctgactgat
     1081 gcactcgatg ccgtccatgc gcggcatggt gagatccatt gtgacaacgt caggatcggt
     1141 gcgcttgaac agctcgatcg cctcgacccc gttacccgcg gtcccgacca gctcgaggtc
     1201 ctcgaactgc tgtgagcgct cgatgcgccg acgcatgatg ttcgagtcgt cgacgatcat
     1261 gagcttcatg ggccgcttgc catccatccc gccgtcctca ggcgaccgcg ctgctgctcg
     1321 cgtggccggc cggcagcgag atcttgaagc gcgtgtactt gcccgggtgc gtgctgacgc
     1381 cgatcttacc gccgagcgcg tagacactgc gcgccaccac gtccatgccg acgccgcggc
     1441 ccgcatccat cgacacggcg tcctgggtgg agaagccggg gcggaagatg agcgccatcg
     1501 cggtgcgcgt gtccatgagg gcagcttctg cctcggtgac cagctgctgg cgcaccgcgg
     1561 cggccttgag cgtatcgggc gcgat
//
LOCUS       DATLKL010000272         1552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_25578, whole genome shotgun sequence.
ACCESSION   DATLKL010000272 DATLKL010000000
VERSION     DATLKL010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25578"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VM713_03560"
     CDS             <1..411
                     /locus_tag="VM713_03560"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HVH83375.1"
                     /translation="SSDRRRSLELLLEQLERGARESQPRLLAEAGSRMHMLDVAQVEV
                     VEADRNYVRLTIGREVFHARSTLSQAERSFQAQPMLRISRSCLVNMSHVKEISRTPRG
                     DFILVCAGGTTVTSSEGFRDPVRQYLERLKLAPA"
     gene            462..>1552
                     /locus_tag="VM713_03565"
     CDS             462..>1552
                     /locus_tag="VM713_03565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH83376.1"
                     /translation="MSTAAPLPRAANRALLVLTGINLLNYLDRYLVPPLVPDLKGAMA
                     LSDQEVGWLWPAFMLVYMVTAPAFGAWGDRGSRRRPIAIGVLIWSAATVLSGLARSYP
                     QLLGARALVGIGEAAYVAIAPALLADCFPLSARGRVYAVLNMAIPVGAALGYVLGGVI
                     GQHAGWRNAFFICGAPGALLAAAVLLIPDPPRGLQDGSEAVRSAPAAEGRGALAVYWS
                     LLRRAPYMLVVLGYAAYTFGLGGLGFWMPAFLERVRGVPPVAATRDFGMVVVLTGIVG
                     TLVGGWLGDYCLRRSRQGYLWLSAAVTLAAAPCALLALTAPAPAEYYPAIVAAELLLF
                     MSTGPIDAAIANIVSPLERASAIALSMFI"
BASE COUNT          196 a          548 c          545 g          263 t
ORIGIN      
        1 agcagcgacc gccggcgctc gctcgaattg ctcctcgagc agctcgagcg cggcgcccgc
       61 gagagccaac cgcgcctgct ggccgaggcc ggcagccgca tgcacatgct ggacgtagcg
      121 caggtcgagg tggtcgaagc cgaccgcaat tacgtacgcc tcaccatcgg ccgcgaggtg
      181 ttccacgcgc gcagcacgct cagccaggcg gagcgttcct tccaggcgca gccgatgctg
      241 cgtatcagcc gctcgtgcct ggtgaacatg agccacgtga aggaaatcag ccgcacgccg
      301 cgcggcgact tcatcctggt gtgcgccggc ggcaccacgg tgaccagcag cgagggcttc
      361 cgcgatccgg tccggcagta tctcgagcgg ctgaaactcg ctccggcctg aggcggttgc
      421 gctaaagtcc cgcgcgcacc tcaccgcgag gggagtaagc attgagtacc gccgcgccgc
      481 tgccgcgcgc cgccaaccgc gcgctgctcg tcctcaccgg catcaacctg ctgaactatc
      541 tcgatcgcta tctggtgccg ccgctggtgc cggacctcaa gggcgcaatg gcgctctcgg
      601 accaggaggt cggctggctg tggccggcgt tcatgctggt gtacatggtc acggcaccgg
      661 ccttcggcgc ctggggtgat cgcggctcgc gccgcaggcc gatcgcgatc ggggtgctca
      721 tctggagcgc ggcgacggtg ctctcggggc tggcgcgcag ctatccgcaa cttctgggcg
      781 cgcgcgcgct ggtcggcatt ggcgaggccg cctatgtcgc gatcgcgccg gcgctgctcg
      841 cggactgctt tccgctgagc gcccgcggac gtgtctacgc ggtgctcaat atggcgattc
      901 cggtgggtgc ggcgctcggc tacgtgctcg gcggcgtgat cggccagcac gccggctggc
      961 gcaatgcgtt cttcatctgc ggcgctccgg gagcgctgct cgccgcggcg gtgctcctca
     1021 tccccgatcc cccgcgcggt ctgcaggacg ggtcggaggc cgtgcggagc gcgcctgccg
     1081 ccgaagggcg gggtgcgctc gccgtctact ggagtctgct gcgccgcgca ccctacatgc
     1141 tcgtcgtgct cgggtacgcg gcctatacgt tcggactcgg aggactgggg ttctggatgc
     1201 cggcgttcct cgagcgcgtg cgcggcgtgc cgccggtcgc ggccacgcgc gacttcggca
     1261 tggtggtggt gctcaccggc atcgtcggca cgctggttgg cggctggctc ggggactact
     1321 gcctcaggcg ctcacggcag gggtatctgt ggctctccgc ggccgtgaca ctcgccgcgg
     1381 cgccgtgtgc gctcctggcg ctcaccgcac cagccccggc tgagtactat ccggcgatcg
     1441 tcgccgccga gctgctgctg ttcatgtcca ccggaccgat cgacgcggcg atcgccaaca
     1501 tcgtctcgcc gctcgagcgc gccagcgcca tcgcgctcag catgttcatc at
//
LOCUS       DATLKL010000273         2939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_36954, whole genome shotgun sequence.
ACCESSION   DATLKL010000273 DATLKL010000000
VERSION     DATLKL010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36954"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="VM713_03570"
     CDS             <1..1507
                     /locus_tag="VM713_03570"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HVH83377.1"
                     /translation="PDELVVPIIEEAKRILAEIGVEVRGARLRERLLAHGLRLAHTAD
                     DAERVLFPPEVVDRAVATAPWSIALFDRDGAPCAELGGDKVHFVPGSSGLHVLDHRSG
                     ETRLSNTRDFIEYVRVADGLKNLGYLATAFSTNDIEPQVSDAWRLYLVLSNSRRPVVS
                     GAFTEHGVPRMAEMMQLFRRDKADLIARPMSIFTVTATGFYRYSEDSCQNLIDCAEFG
                     IPVEIVPVTLMGLIAPVTLVGATVMHVADVLAGLTMAQMVRPGTPVLFGGAPATFHMK
                     TAMAPMAAIETLHLNVAYVEIAKHLGLPTQGYMALSEGKFLDAQAGAETFAGALLAAI
                     SGVNSVSGPGMLDYVLTFSLEKLVFDDELCGQALHFCREFAVMEDLPTLDLVRQLLAE
                     KHLLTAGHTLSYWPRELYLPGPVFDRINRETWLQQGGRSLDQRVKAKVERLLGAYVSP
                     RTDAHIEAELIALIKADLTTEQPLPQTLPPVEQPSGQGEREGRRARRSRRG"
     gene            complement(1523..2557)
                     /locus_tag="VM713_03575"
     CDS             complement(1523..2557)
                     /locus_tag="VM713_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVH83378.1"
                     /translation="MSSRREFLKAGAVTAGVAGAAAASATTGKTSMAKKYSGPHEMPT
                     GVTLLSLRNADGSETLGAKLGADTVLDVRKAAQLLGMTAPLTLEELLKEGNARGLNEL
                     IAAAQTSRRADALMVQESSITFGRLFTSPGKIVCIGLNYRAHAAETNLKLPSVPILFN
                     KYNNALAPHNCTIALPPREVSYKFDYETELLIVMGRSARNVPEGEALDYVAGYCTAHD
                     FSARDLQLETPSVQWMIGKTLDNFGPIGPYFVSADRVGDPNNLKLETHVNGELRQSSN
                     TSFMIFNPQKLIAYITRMWTLEPGDIIWSGTPEGVILGMPKEKQVWLKAGDEIVSTIE
                     KLGSLRFRLA"
     gene            complement(2565..>2939)
                     /locus_tag="VM713_03580"
     CDS             complement(2565..>2939)
                     /locus_tag="VM713_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HVH83379.1"
                     /translation="VVTYDITAQFTHWIMYNIPADTTSLPENAGAPGSTFGVQNENDF
                     FQPPPSYNGPCPPATLMPFSHQYQFTVYALDRMLPLVPTFGDFVPAGPEGFYQELIAA
                     SREGHVLASASISGFFSSAPGN"
BASE COUNT          465 a          932 c         1020 g          522 t
ORIGIN      
        1 ccccgatgag ctggtcgttc cgatcatcga ggaagcgaag agaatcctcg ccgagatcgg
       61 ggtcgaggtg cgcggggcgc ggctgcgcga gcggctgctg gcgcacgggc tgagactggc
      121 ccacaccgcc gacgacgcgg agcgggtgct gtttccgccc gaggtggtcg accgtgcggt
      181 cgcgactgcg ccgtggagca ttgcgctctt tgaccgcgac ggcgcgccct gtgccgagct
      241 gggcggcgac aaggtgcact tcgttcccgg ctcgagcggc ctgcacgtgc tcgatcaccg
      301 cagcggtgag acacgtctct ccaatacgcg cgacttcatc gagtacgtac gcgtagcgga
      361 tggcctgaag aacctcggct acctggcgac cgcgttctcc accaatgaca tcgagccgca
      421 ggtatctgac gcctggcggc tgtatctggt gctctccaac tcgcgccggc cagtggtctc
      481 gggcgccttc accgagcacg gtgtgccgcg catggcggag atgatgcagc tgtttcgccg
      541 cgacaaggcc gatctcatcg cgcgtccgat gtcgatcttc accgtcacgg ccaccggctt
      601 ctaccgctac agcgaagact cgtgccagaa cctcatcgac tgcgccgagt tcggcattcc
      661 ggtggagatc gtgcccgtga ccctcatggg actgatcgcg ccggtgacac tcgtcggcgc
      721 gaccgtcatg cacgtcgccg atgtgctggc gggactgacg atggcgcaga tggtgcggcc
      781 cggcacgccg gtgctgttcg gcggcgcgcc cgccaccttc cacatgaaga cggccatggc
      841 gcccatggcg gcgatcgaga cgctgcacct caacgtggcc tacgtggaga tcgccaagca
      901 cctcggcctg ccgacgcagg gctacatggc cctgagcgaa ggcaagtttc tcgacgcgca
      961 ggccggggcc gagaccttcg cgggcgcgct gctcgccgcg atcagcggtg tcaattcggt
     1021 gtccgggccg ggaatgctcg attacgtgct gactttcagc ctggagaagc tcgtgttcga
     1081 cgacgagctg tgtgggcagg cgctgcattt ctgccgcgag tttgccgtca tggaggatct
     1141 gccgaccctc gatctggtgc gccagctcct ggcggagaag catttgctca ccgccgggca
     1201 caccttgagc tactggccgc gcgagctgta tctgccgggg ccggtgttcg accggatcaa
     1261 ccgcgagacc tggctgcagc agggtggcag gagcctcgat cagcgcgtca aggccaaagt
     1321 cgagcggctg ctcggcgctt acgtctcgcc ccgcaccgac gcgcacatcg aggcggagct
     1381 gatcgcgctc atcaaggcgg atctgacgac cgagcagccg ctgccgcaga ctctgccgcc
     1441 ggtggagcag ccgagcggac agggtgagcg cgaggggcga cgcgcgcggc ggagccgccg
     1501 gggttagggc gccgacgagc ggctacgcca ggcggaacct gagcgagccg agcttctcga
     1561 tcgtgctcac gatctcgtcc cccgccttga gccacacctg cttttccttg ggcatgccga
     1621 ggatgacgcc ctccggggtt cccgaccaga tgatgtcgcc gggctcgagg gtccacatcc
     1681 gggtgatgta ggcgatgagc ttctgcgggt tgaagatcat gaagctggtg ttagacgact
     1741 ggcgcagctc gccgttcacg tgtgtctcga gcttgaggtt gttggggtca ccgacgcggt
     1801 cggccgacac gaagtagggg ccgatggggc cgaagttatc gagcgtcttg ccgatcatcc
     1861 actgcacgct cggggtctcg agctgcaagt cgcgcgcgga aaagtcatgc gcggtgcagt
     1921 accccgccac atagtcgagc gcctctccct cgggcacgtt gcgggcgctc ctgcccatga
     1981 cgatcagcag ctccgtctca tagtcgaact tgtaggacac ctcacgcggc ggcagcgcga
     2041 tggtgcagtt gtgaggggcg agcgcgttgt tgtacttgtt gaagaggatc ggaacgctgg
     2101 ggagcttgag gttcgtttcc gcggcgtgcg cccggtagtt cagcccgatg cagacgatct
     2161 tgcccgggct cgtgaagagc ctgccgaagg tgatggacga ttcctgcacc atcagagcat
     2221 cggcccggcg cgaggtctgc gccgcggcga tgagctcgtt gagcccgcgc gcgttacctt
     2281 ccttgagcag ctcctcgagc gtcagcggcg ccgtcatccc gaggagctgc gcggccttgc
     2341 gcacatcgag cacggtatcg gcgccgagct tggcgccgag cgtctccgag ccgtcggcgt
     2401 tgcgcagcga cagcagcgtc accccggtcg gcatttcgtg cgggccgctg tatttctttg
     2461 ccatggaggt cttcccggtc gtggcggagg ccgcggcggc gccggctacg ccggcggtca
     2521 ccgcgcccgc cttgagaaac tcccgtctcg agctcatgga acgatcaatt gcccggcgcg
     2581 gacgagaaga agccggagat actggcgctc gcgagcacat gcccctcgcg gctcgcagcg
     2641 atcagctcct ggtagaagcc ttccggcccg gccggaacga aatcgccgaa ggtcggtacc
     2701 agcggcagca ttcggtcgag cgcatagacg gtgaactggt actgatgcga gaacggcatc
     2761 agggtcgcgg gcgggcaggg gccgttgtag cttggcgggg gctggaagaa gtcgttctcg
     2821 ttctgcaccc cgaaggtgct gcctggcgct ccggcgttct ccggcaggct ggtcgtgtcc
     2881 gccggtatgt tgtacatgat ccagtgagtg aactgggcgg tgatgtcgta agtgaccac
//
LOCUS       DATLKL010000274         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_3100, whole genome shotgun sequence.
ACCESSION   DATLKL010000274 DATLKL010000000
VERSION     DATLKL010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3100"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2551
                     /locus_tag="VM713_03585"
     CDS             <1..2551
                     /locus_tag="VM713_03585"
                     /inference="COORDINATES: protein motif:HMM:NF009586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVH83380.1"
                     /translation="ESMLGAIVTLLAVLDAAYAVTQVGGILTLVFIGAVLFLAYRRLS
                     LLAFTVTFTVLLALYTAIGAPAGIWKGLLWVLLAGLWLLNVRPLRKVLITRPFLATYL
                     RLLPSMSQTEKEALEAGTVWWDGELFTGAPDWPKLLAAKPPQLSPEEQAFLDGPCEEL
                     CRMLDEWHITHERGDLPPEAWEVLKTRGFFAMIIPKRYGGLEFSAYAHSCVLAKISSR
                     SPTAASNAAVPNSLGPAELLNHYGTEEQKNYYLPRLARGEEVPCFALTGPRAGSDAAS
                     IPDTGVVCRGMWQRSETVGLRLNFSKRYITLAPVATVIGLAFRMFDPDRLLGAKTDIG
                     ITCALIPRNTPGITIGRRHFPLNVPFQNGPIQGRDVFVPLDFIIGGPAMAGSGWRMLV
                     EQLSVGRCISLPSNTTGAAKAAVWATGAYARIRSQFNTPVGKFEGVEAVIARMVGLTY
                     TMDAGRSVTAGAIDGGEKPSVPSAMLKYHVTEMGRQVANDAMDVHGGKGIMLGPKNYL
                     GRGYEITPVAITVEGANLLTRNLIIFGQGAIRCHPFVLREMNAARNPDRTAGVDAFDR
                     ALFGHIGFTISNAVRSFIMALTHARFTRVPVAGGTARYYQHIVRFSASFAFAVDVAML
                     TLGGYLKKKEGLSARLGDVLSSMYLASMVLKHHENQGRRAEDLPIVEWACRNLLYQAQ
                     EQLHGFQRNFPNRVLAAMMRALIFPRGLVYSAPGDGLAHRVAALATAPTEAREWLCLG
                     IYWTLEPGNPLGLLQEAMLLAGQLEPLLKTIRVEGVKTGKVTALDLPGQIGQAHACGI
                     VSETEAAALREYDRKIMDLINVDDFASEELGTQAQPLEAQPAGGQSRPYVA"
     gene            2548..>3128
                     /locus_tag="VM713_03590"
     CDS             2548..>3128
                     /locus_tag="VM713_03590"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HVH83381.1"
                     /translation="MSTPASAVQPVAVGPPAAPSEPAAPALVAPTALGPPPTVPATGV
                     TRCANCGASVPARYCGNCGQRLEPPVHSLVHFLKVAAEDVTHADSRLWRTLWALLFKP
                     GFLTREFLDGRRASYLPPVRLYLVLSVVFFIWFGATEQKMTVVQVDAPRTAGGAAAVV
                     PGRREFGPLAPAQPGESPEQRADRQCPKANYEGP"
BASE COUNT          455 a         1129 c         1024 g          520 t
ORIGIN      
        1 agagtccatg ttaggtgcga tcgtcactct gctcgcggta ctggacgccg cctacgcggt
       61 cacgcaggtc ggcggcatcc tcaccctggt gttcatcggc gcggtgctgt tcctcgccta
      121 ccggcgcctg tcgctgctcg ccttcacggt caccttcacg gtgctgctgg cgctctacac
      181 cgccataggc gccccggcag gcatctggaa ggggctgctg tgggtgctgc tcgcggggct
      241 gtggctcctc aatgtgcggc cgctgcgcaa agtgctcatc acccgcccct tcctggccac
      301 ctatctcagg ctcctgccgt cgatgtcgca gaccgagaag gaagcactcg aggcaggcac
      361 ggtgtggtgg gacggcgagc tgttcacggg ggcgcctgac tggcccaagc tcctcgccgc
      421 gaagccaccg cagctctcgc cggaggaaca ggcgttcctc gacggtccgt gcgaggagct
      481 ctgccggatg ctcgatgagt ggcatatcac ccacgagcgc ggcgacctgc caccggaggc
      541 ctgggaggtc ctcaagacgc gcggcttctt cgcgatgatc atcccgaaga ggtacggcgg
      601 actcgagttc tccgcctatg cgcactcgtg cgtgctcgcc aagatctcga gccggagtcc
      661 caccgccgcg tccaacgccg cggtgcccaa ttcgctcgga cccgcggagc tgctgaacca
      721 ctatggcacc gaagagcaga agaactacta cctgccgcgc ctcgcgcgcg gcgaggaagt
      781 gccgtgcttt gcgctcaccg gtccgcgcgc cggttccgat gccgcctcca tccccgacac
      841 cggggtcgtg tgccgcggca tgtggcagcg gtctgagacc gtcggcctca ggctcaactt
      901 ctccaagcgc tacatcacgc tcgccccggt cgcgaccgtg atcgggctcg ccttccgcat
      961 gtttgatccg gaccggctcc tcggcgccaa gaccgacatc ggcatcacct gcgcgctcat
     1021 tccgcgcaat acgccaggca tcacgatcgg gcggcgacac tttccgctca acgtgccgtt
     1081 ccagaacggc cccatccagg gtcgcgacgt gttcgtgccg ctcgacttca tcatcggcgg
     1141 tccggccatg gcgggcagcg gctggcgcat gctggtcgag cagctctccg tgggccgctg
     1201 catctcgctg ccctccaaca ccaccggggc ggccaaggcc gcggtgtggg cgaccggcgc
     1261 gtacgcgcgc atccgcagcc agttcaatac gccggtcggc aagttcgagg gagtcgaggc
     1321 ggtgatcgcc cgcatggtcg gcctcaccta caccatggac gcggggcgct cggtgaccgc
     1381 gggcgcgatc gacggcggcg agaaaccctc ggtcccctcc gcgatgctca agtatcacgt
     1441 gaccgaaatg ggccgccagg tcgccaacga cgccatggac gtgcacggcg gcaagggcat
     1501 catgctcggg ccgaagaact atctcggacg cggctatgag atcacgccgg tcgcgatcac
     1561 cgtcgagggc gcgaacctcc tcacccgcaa cctgatcatc ttcggccagg gcgccatccg
     1621 ctgccatccg ttcgtgctgc gcgagatgaa tgcggcgcgc aatcccgacc gcacggcggg
     1681 cgtcgatgcg ttcgatcgcg cgctgttcgg gcacatcggc ttcaccatct ccaacgcggt
     1741 gcgctccttc atcatggcgt tgacccacgc gcgcttcacg cgcgtgccgg tcgcgggcgg
     1801 cactgcacgc tattaccagc acatcgtgcg cttcagcgcc tcgttcgcct tcgcggtgga
     1861 tgtcgcgatg ctgaccctcg gcggctatct caagaagaag gagggcctgt cggcgcgcct
     1921 cggcgacgtg ctctcctcga tgtacctcgc ctcgatggtg ctcaagcatc acgagaacca
     1981 ggggcggcgc gccgaggatc tgccgatcgt cgagtgggcc tgccgcaatc tcctctacca
     2041 ggcgcaggag cagctgcacg gctttcagcg taatttcccc aaccgcgtgc tcgccgcgat
     2101 gatgcgcgcg ctcatcttcc cccgtggcct ggtctattcc gccccgggtg acgggctggc
     2161 tcaccgcgtg gccgcgctcg ctaccgcgcc gaccgaggcg cgtgagtggc tctgtctggg
     2221 catttactgg acgctcgagc ccggcaatcc gctcggcctc ctgcaggagg cgatgctgct
     2281 cgcggggcag ctcgagccgc tcctgaagac catccgcgtc gagggcgtca agaccggcaa
     2341 ggtcaccgcg cttgatctcc cgggccagat cggccaggcg cacgcctgcg gcatcgtctc
     2401 cgagaccgag gcggcggcgc tgcgcgagta cgaccgcaag atcatggacc tcatcaacgt
     2461 cgatgacttc gcctccgagg agctcggcac ccaggcgcag ccgctcgaag cgcagccggc
     2521 cggcgggcag tcgcgccctt acgtcgcatg agtactccgg cgagcgccgt gcagccggtc
     2581 gcggtgggac cgcccgcggc gccgagcgag ccggccgctc ccgccctcgt ggcacccacc
     2641 gcgctcgggc cgcccccgac cgtgccggcg acgggcgtta cgcgctgcgc caactgcggc
     2701 gcgagcgttc cggcccggta ctgcggcaac tgtggccagc gcctcgagcc gccggtgcac
     2761 tcgctggtgc atttcctcaa ggtggcggcc gaggatgtca cgcacgccga ttcgcggctg
     2821 tggcgcacac tctgggcgct gctcttcaag cccgggtttc tcacccgcga gttcctcgac
     2881 ggccggcgcg ccagctatct gccgccggtg cggctgtacc tggtgctgtc agtggtgttc
     2941 ttcatctggt tcggcgccac tgagcagaag atgacggtcg ttcaggtcga tgcgccgcgc
     3001 accgccggcg gcgctgccgc ggtcgttccc gggcggcgcg agtttggccc gctggcgccg
     3061 gcgcaacccg gcgaatcccc ggagcaacgg gccgaccgcc agtgcccgaa agccaactac
     3121 gagggtcc
//
LOCUS       DATLKL010000275         1907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_59488, whole genome shotgun sequence.
ACCESSION   DATLKL010000275 DATLKL010000000
VERSION     DATLKL010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59488"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VM713_03595"
     CDS             complement(<1..396)
                     /locus_tag="VM713_03595"
                     /inference="COORDINATES: protein motif:HMM:NF025759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK-like ATPgrasp N-terminal domain-containing
                     protein"
                     /protein_id="HVH83382.1"
                     /translation="MTAHIVVVDRRADFKWDDSGHPVVTAREYLARPDAFMSRYPRII
                     NLCRDYEYMSFGYYCSLLAEARGERVIPSVSTILDLSKKSIYGLALDELDELLDKTAK
                     KYGQQPQEPITLHVFFGFTEEERYQELAHQ"
     gene            complement(393..1565)
                     /locus_tag="VM713_03600"
     CDS             complement(393..1565)
                     /locus_tag="VM713_03600"
                     /inference="COORDINATES: protein motif:HMM:NF023242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase/peptidase C39
                     family protein"
                     /protein_id="HVH83383.1"
                     /translation="MPPRRRRHDAAVMALRKPPAIQLAIRPARLDDIPALLALENSAF
                     VTDRVSARSFRHLLTRANAATLVEEHRGVLRGYATLLFNRASSLARLYSFAVSPDHRG
                     LGIAKALLAEAERVALARGCVYLRLEVRKDNFEAKELYRRMGYREFGVAADYYDDHMD
                     AIRMEKPLSASLKPALLRVPYSPQTLEFTCGPAALMMAMHALDPRIPLDRTLELRIWR
                     ESTTIFMTTGHGGCSPFGMALSAVARGFAVEVYVNDRGILFEDTVRKPEHKEVIRLVQ
                     EDFLAEMREKGVGLHYGRLGVEDLECESEAGGIPIVLISSFRIDRDREPHWVVVTGFD
                     ERFIYVHDPFVDWKGHRTTTDCVNMPIAKNDFERMARFGRAQQKAALVIRKRKGGA"
     gene            complement(1598..>1907)
                     /locus_tag="VM713_03605"
     CDS             complement(1598..>1907)
                     /locus_tag="VM713_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVH83384.1"
                     /translation="QKPSKHWIEGLAPLKVPCSPVNTLDQVFADAHIQHRRMVVEMDH
                     PLGGRPVRLIANPIKMSATRPEYRYAPPILGQHTDEVLSEVLGLRAKEIARLREKGVI
                     "
BASE COUNT          329 a          619 c          627 g          332 t
ORIGIN      
        1 ctggtgcgcg agctcctggt agcgctcctc ctcggtgaag ccgaagaaga cgtggagcgt
       61 gatcggctcc tgcggctgct gaccgtactt cttcgccgtc ttgtcgagaa gctcgtcgag
      121 ctcgtcgagc gccaggccat agatcgactt cttggagagg tcgaggatgg tcgaaaccga
      181 agggatgacc cgctcgcccc gcgcctcggc caggagcgag caataatagc cgaagctcat
      241 atactcgtaa tcccggcaga ggttgatgat gcgcggatag cgcgacatga aggcgtccgg
      301 ccgcgcgagg tactcccggg cggtcacgac cgggtgaccc gagtcgtccc atttgaagtc
      361 cgcgcgccgg tcgacgacga caatgtgagc cgtcatgcgc cgcccttgcg cttgcggatc
      421 accagagctg ccttttgttg agctcgcccg aaccgggcca tgcgctcgaa gtcattcttg
      481 gcgatcggca tgttgacgca gtcggtcgtg gtccggtgac ctttccagtc cacaaaggga
      541 tcatgcacgt aaatgaaacg ctcgtcgaat cctgttacga ccacccaatg gggctcgcgg
      601 tcccggtcga tgcggaacga gctgatcagg acgatgggga ttccgccggc ctcggactcg
      661 cactcgaggt cctcgacgcc gagtcgcccg taatgcagcc ccaccccctt ttcccgcatc
      721 tcggcgagaa aatcctcctg gaccaggcga atcacctcct tgtgctcggg cttgcgcacc
      781 gtgtcctcga acaggatgcc gcggtcgttg acgtagacct cgacggcgaa gccgcgcgcc
      841 accgccgaca gcgccatccc gaacgggctg cagccaccgt ggcccgtggt catgaagatg
      901 gtggtcgact cacgccagat gcgaagctcg agcgtgcggt cgagcggtat gcgcgggtca
      961 agcgcgtgca tcgccatcat aagggcggcg gggccgcagg tgaactcgag cgtctgcggg
     1021 gagtagggga cgcgcaggag cgccggcttg aggctcgcgg agagcggctt ctccatgcgg
     1081 atcgcgtcca tgtggtcgtc atagtagtcg gccgccacgc cgaactcgcg gtagcccatc
     1141 cgccgataga gttccttggc ctcgaaattg tccttgcgca cctcgagcct cagatagacg
     1201 cagccgcgcg cgagtgcaac cctctccgcc tcggcgagca gcgccttggc gatgccgagg
     1261 ccgcgatgat cgggggaaac cgcaaaggag tagaggcggg ccagcgagct cgcgcggttg
     1321 aacagcagcg tggcatagcc cctgagcacc ccccggtgct cctcgacgag ggtggcggcg
     1381 ttcgcgcggg tcagcaggtg ccggaaactg cgagcgctga cccggtcggt gacaaacgcg
     1441 ctgttctcga gcgccaacag cgccgggatg tcgtcgagcc tagccggtcg gatggccagc
     1501 tggatggccg ggggcttgcg cagcgccatc accgccgcat cgtgccggcg ccgcctcgga
     1561 ggcacgggca atgtcctctc ctcaaccgca cgcgagctca aatcactcct ttctccctga
     1621 gccttgcgat ttccttggcc cgaaggccca gcacttcgct caggacctcg tcggtgtgct
     1681 ggccgagtat cggcggggcg taacggtact cgggccgggt tgccgacatc ttaatgggat
     1741 tggcaatcag ccgcaccggc cgccctccca gagggtggtc catctcgacc accatccgcc
     1801 gatgctgaat atgagcgtcg gcgaacacct ggtccagggt gttgaccggg ctgcacggca
     1861 ccttgagcgg ggccaggccc tcgatccaat gcttcgacgg cttctgg
//
LOCUS       DATLKL010000276         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_93345, whole genome shotgun sequence.
ACCESSION   DATLKL010000276 DATLKL010000000
VERSION     DATLKL010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93345"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VM713_03610"
     CDS             <1..318
                     /locus_tag="VM713_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83385.1"
                     /translation="LSGFADSLGFFYASKIWQGGLFSWIDLARSAAGWAVGISLYIIA
                     LKPMSSVGVTSAEMQTTVWFAMTIIGVVLFSGRFFTWQWLDQLVAVLAVVALGCLLVR
                     TGG"
     gene            350..991
                     /locus_tag="VM713_03615"
     CDS             350..991
                     /locus_tag="VM713_03615"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HVH83386.1"
                     /translation="MMLIPLTDASRRPTDFPLVTAVIIGVNALVFVLELLGGDAFVLR
                     WSVVPADIVAGRHWITLLTGMFMHASWSHIIGNMVFLWAFGPEVEEQMGRGRYLVFYL
                     LGGLAAAFAQLIASPASTVPNLGASGAIAAVMGAFLVTYPHDQIRSLLVIFVFVHVTY
                     IPAALLIGAWGLLQLVSLGAVVQHPAAGGVAYAAHVGGFIFGAVVARLFTRPP"
     gene            complement(1033..1638)
                     /locus_tag="VM713_03620"
     CDS             complement(1033..1638)
                     /locus_tag="VM713_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83387.1"
                     /translation="MKRGSCVLGFAPHSGWAAVVVIGGTRSKPLVLARERLELADETL
                     PGARQPYHAIEKLPLPEASARLARLTAAATALATVGLRKLLRTARAAGVEPVAAAILD
                     SSGRDSASLAAILASHALIHTADGNHFREALASACAAESLPCTRIPRRDLPDEAARAL
                     KRAPAELTAAVARLGRDVGAPWGADQKGAALVAWLLLARGM"
     gene            complement(1667..>2530)
                     /locus_tag="VM713_03625"
     CDS             complement(1667..>2530)
                     /locus_tag="VM713_03625"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HVH83388.1"
                     /translation="NERYLPSAPFPDALEVEADLSAALAAARYVLIVVPSPAFRAVLG
                     ALAPHLEPEMHVAWATKGFELATGLLPHQVACEVLGSERRVAVLSGPTFAREVGAGLP
                     TAMTVASPNAAYAEALAAELSSANFRAYTSTDIMGVEIGGAVKNALAVGAGLSDGLGF
                     GANTRVALITRGLKEMTRLGVALGADAHTFMGLAGLGDLVLTYTDDQSRNRRFGLKLA
                     AGASPEAALAEIGQSVEGYAAARAIHTVAARTAVEMPICEAVYRVLYEALPARDAVRE
                     LMSRPLKAEVA"
BASE COUNT          353 a          882 c          874 g          421 t
ORIGIN      
        1 ctgtcgggct tcgcggattc gctcggtttc ttctacgcct cgaagatctg gcagggcgga
       61 ctcttctcgt ggatcgatct cgcgaggagc gcggccggct gggcggtcgg catctcgctc
      121 tacatcatcg cgctgaagcc catgtcgagc gtcggtgtga cttccgcgga gatgcagacc
      181 accgtgtggt ttgccatgac catcatcggc gtcgtcctct tcagcggacg cttctttacc
      241 tggcagtggc tcgatcagtt ggttgcggta ctggccgtcg tggctctcgg atgtctcctc
      301 gtgaggacgg gtggctgaag cgggccagat tcagtcgagg tgactcaaaa tgatgctcat
      361 tccgctcacc gatgcttccc gccgcccgac cgacttcccg ctcgtgaccg cggtgatcat
      421 cggcgtaaac gcgctggtct tcgtgctgga gctgctcggc ggagacgcct tcgtcctgcg
      481 ctggtcggtg gtcccggccg acatcgtcgc cgggcgtcac tggatcacgc tcctcacggg
      541 catgttcatg catgccagct ggtcgcacat catcggcaac atggtgtttc tctgggcgtt
      601 cgggcccgag gtcgaggagc agatggggcg gggtcgctat ctcgtgttct atctcctcgg
      661 gggcctcgcg gccgcgttcg cccagttgat cgcgagcccg gcatccaccg tgccgaacct
      721 cggcgcgagc ggcgcgatcg ccgccgtgat gggcgccttc ctcgtgacct atccgcacga
      781 ccagatccgc tcgctgctgg tgatcttcgt gttcgtgcac gtcacttata ttccggcggc
      841 gctcctcatc ggcgcgtggg gcctgctgca gctggtgagt ctcggcgccg tcgtgcagca
      901 ccccgccgcg ggcggcgtgg cttatgccgc gcacgttggc ggcttcattt tcggcgcggt
      961 cgtcgcgcgg ctcttcacac gcccgccgtg agctgccggc ttgcagggca cgagccgccg
     1021 gcgctcacca tttcacatac cgcgcgcgag cagcagccac gccacgagcg cggcgccctt
     1081 ctggtcggcg ccccacggcg cgccgacgtc gcgtccgagg cgcgccaccg ccgccgtgag
     1141 ctccgccggc gcgcgcttga gcgcccgcgc ggcctcgtcg gggagatccc gccgcgggat
     1201 gcgtgtacag ggaaggctct cggccgcgca cgccgaggcc agtgcctcgc ggaagtggtt
     1261 gccgtcggcg gtgtgaatca atgcatgcga ggccaggatc gccgcgagcg aggctgagtc
     1321 gcgccccgag gaatcgagga tggcggcggc caccggctcg actcccgccg cgcgcgcggt
     1381 ccgaagcagc ttacgaagcc cgacagtggc gagcgcggtc gccgcagcgg tgagccgggc
     1441 gagccgtgcg ctcgcctccg gcagcggcag cttctcgatc gcgtggtaag gctgcctcgc
     1501 acccggcaga gtctcatcgg cgagctcgag ccgctcgcgt gccagcacca gcggcttgct
     1561 gcgcgtgccg ccgatgacga cgaccgccgc ccatcccgaa tggggcgcga atcccaatac
     1621 gcacgaccca cgcttcacgg cggcagacaa gcggcggctg cggcggttaa gcgacttccg
     1681 ccttgagcgg gcggctcatg agctcgcgca ccgcatccct agccggcaga gcctcgtaga
     1741 gcacccggta caccgcctcg cagatcggca tctcgacggc ggtgcgggcc gcgacggtgt
     1801 gaatggcgcg cgccgccgcg tagccttcga ccgactggcc gatctcggcg agcgcggctt
     1861 caggacttgc gcccgcggcg agcttcaggc cgaagcgacg gttgcgggac tgatcgtcgg
     1921 tgtaggtgag caccaggtcg ccgagtcccg caagtcccat gaaggtatgc gcgtcggcgc
     1981 cgagcgcgac ccctaagcgt gtcatctcct tcagtccccg cgtgatgagc gcgacgcgcg
     2041 tgttggcgcc gaagccgagc ccgtcggaaa gccccgcccc caccgcgagc gcgttcttca
     2101 ccgcgccgcc gatctcgact cccatgatgt cggtcgaagt gtaggcgcgg aaattcgccg
     2161 agctcaattc ggcggcgagc gcctcggcgt aagcggcatt aggcgaggcg acggtcatgg
     2221 cggtcggcag tcccgcgccg acctcacgcg cgaacgtcgg gcctgagagc acggcgacgc
     2281 gccgctcgga cccgaggacc tcgcacgcca cctgatgcgg caggagcccc gtcgcgagct
     2341 cgaatccctt ggtcgcccac gccacgtgca tctcgggctc aaggtgcggg gcgagtgcgc
     2401 cgaggacggc gcgaaacgcc ggactcggca cgacgatgag cacatagcgc gccgcggcga
     2461 gcgccgccga caggtcggcc tccacctcga gcgcatcggg gaagggcgcg ctcggcagat
     2521 agcgctcgtt
//
LOCUS       DATLKL010000277         1762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_3120, whole genome shotgun sequence.
ACCESSION   DATLKL010000277 DATLKL010000000
VERSION     DATLKL010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3120"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VM713_03630"
     CDS             complement(<1..750)
                     /locus_tag="VM713_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVH83389.1"
                     /translation="MRLLPIVSLALAAAAAPAAAVATGDGEVLRATLDNGLRVIVVRN
                     SLSPVVTTSVNYLVGSDEAPAGFPGTAHAEEHMMFRGSPGLTADQLADIGSVLGGDFN
                     ADTREGLTQYLFTVPAEDLDVALHIESLRMRAALNTVKEWSQERGAIEQEVAQDLSQP
                     GYVLYSKLRARLFAGTPYAHDALGTRPSFQKTTAAMLKTFHETWYAPNNAILVIAGDV
                     DPETALGEVKALFGDIAPKKLPANPRCALARC"
     gene            960..1670
                     /locus_tag="VM713_03635"
     CDS             960..1670
                     /locus_tag="VM713_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVH83390.1"
                     /translation="MITRNARYLRYGLAAVACLTGAPVWADAAAAPDSNAKAEDIGIF
                     TGLAVGAAAGGPIGAVIGAAAGGLMGDRYHREKQTARELKSDLGKSEAERARLEHTLA
                     AVDAVGMDVSFRTDDSSIKVEDMGPLLKLGALAAAVPDAQLRVAGFADPRGDAAYNEA
                     LSQQRAEAVAAALVCGGMARERLIIEAHGASESTSAAGDLDAFALDRRVTVRLERATP
                     TPPAKVASSGGLVPGTGP"
BASE COUNT          267 a          591 c          641 g          263 t
ORIGIN      
        1 gcaccgggcg agggcacacc gggggttcgc gggcagcttc ttcggcgcga tgtcgccgaa
       61 gagcgccttg acctctccga gcgccgtctc ggggtccacg tcgcccgcga tcaccaggat
      121 ggcgttgttg ggcgcatacc aggtttcgtg gaaggtcttg agcatcgccg cggtcgtctt
      181 ctggaacgag ggccgcgtgc cgagcgcgtc gtgcgcatac ggcgtgccgg caaagagccg
      241 ggcgcgcagc ttcgagtaca gcacatagcc gggctgggac agatcctggg cgacctcctg
      301 ttcgatcgcg ccgcgctcct ggctccactc cttcaccgtg ttgagcgccg cgcgcatgcg
      361 cagcgattcg atgtgcagcg ccacgtcgag gtcctcggcg ggaacggtga acaggtactg
      421 cgtgagcccc tcgcgcgtgt cggcattgaa gtccccgccg aggacgctgc cgatgtcggc
      481 aagctgatca gcggtgagtc ccgggctgcc gcggaacatc atgtgctcct cggcgtgggc
      541 ggtcccgggg aagcccgccg gcgcctcgtc ggagccgacc aggtagttca cggaggtcgt
      601 gaccacgggc gagagggagt tgcgcaccac gatgacgcgc agcccgttgt cgagcgtggc
      661 ccgcagcacc tcgccgtcgc cggttgccac ggcggcggcg ggcgcggccg cagccgccag
      721 ggcgagcgag acgatcggga gcagccgcat gactcattct tccggtggcg gggggtgcgc
      781 agtatagcga agcgccgcgg cggcgacgcc cgtgcgcccc ggccgccatg ttctgccata
      841 aagcatcccg tccgctgacg tctttgcatc atcggcgtgg cccgcgcgcg cgctgaacta
      901 tctctcgtac aggaccgctg cgattgcggt cgaccatcta ccaaacgaga ggaggcaaca
      961 tgattacgcg gaacgcgaga tacctgcgct acgggctggc agccgtggcc tgcctgactg
     1021 gcgcacccgt ctgggcggac gccgccgccg cgcccgacag caacgccaag gcggaggaca
     1081 tcggcatctt caccgggctc gccgtcggcg cggcggccgg tggacccatt ggcgccgtga
     1141 tcggcgcggc ggccggcggt ctcatgggcg atcgctatca ccgcgagaag cagacggccc
     1201 gggagctgaa gtcggatctc ggcaagagcg aggccgagcg cgcgcgactc gagcacacgc
     1261 tggccgcggt ggatgcggtc gggatggacg tcagcttccg caccgacgac tcctcgatca
     1321 aggtcgagga catgggaccg ctgctgaagc tcggcgccct ggcggcggcc gtcccggacg
     1381 cgcagctgcg cgtcgcgggg ttcgccgatc cgcgcggcga tgccgcctac aacgaggcac
     1441 tgtcgcagca gcgcgccgag gcggtggcag ccgcactcgt ctgcgggggc atggcgcgtg
     1501 agcggttgat catcgaggca cacggtgcga gcgagtcgac cagtgcggcc ggcgatctcg
     1561 acgcctttgc cctcgatcgg cgggtgacgg tgcgtctcga gcgtgcgacg cccacaccac
     1621 cggcgaaggt cgcaagctcc ggcggcctcg tccccgggac cggtccgtga ggggtgcggc
     1681 gcggcgaaag catcggagag ccccatccaa atccccatgt tggccataga gggtccccag
     1741 actcgtcagt ttttcggcat ta
//
LOCUS       DATLKL010000278         1667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_149874, whole genome shotgun sequence.
ACCESSION   DATLKL010000278 DATLKL010000000
VERSION     DATLKL010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149874"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1048
                     /gene="argB"
                     /locus_tag="VM713_03640"
     CDS             110..1048
                     /gene="argB"
                     /locus_tag="VM713_03640"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HVH83391.1"
                     /translation="MIMHRGDQTLAIRALKSAAPYIRLYKGKTFVVKAGGGVFAAEDS
                     TRSLVEQIGILHYFGVRVVLVHGGGPQLAELSAALGVPTQMVEGRRVTDQRSIEVTAM
                     VLNGLINTRLLAMCRDLNIDAVGISGVDAGLVRAHKRPPVRLTAGGDPVDFGYVGDID
                     AVDTTVLRKLLDNGLMPVVSPLSADARGTLLNINADTVAAALGAALGAEKLILCTATP
                     GILGSVTDPGSLISYTDLAGLKRLRESGQIADGMLPKAKAIEDAIRGGVRRVHVVSYR
                     APEGILGEVFTNEGTGTLIVADINALSPAEQQGTLP"
     gene            1045..>1667
                     /locus_tag="VM713_03645"
     CDS             1045..>1667
                     /locus_tag="VM713_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HVH83392.1"
                     /translation="MTRLWDKGAPLDERVLRYTAGADYALDERLVRYDVRGSIAHAEM
                     LNARELLPLTDLDAIRDGLEALAAEHARGLWHVELADEDGQTALERRLTERIGPAGAR
                     IHLGRSRNDQVLTAIRLYLRGTTEDLESGALQVAEALDELGVREGGTALPGYTHMQQA
                     MPSSVPLWAGGFAAEIRDDAQGLVEVARRLSRSPLGSAAGYGTPGLP"
BASE COUNT          250 a          606 c          577 g          234 t
ORIGIN      
        1 ctcgacggcc cgcgcagcgc cgtgcagcgc gaggccgcca accgcctcag cgtgcagatg
       61 gcggtgctcc atcagctgct gaggccgtga agactcggga aaccgcatca tgatcatgca
      121 tcgaggcgat cagactctcg cgatccgcgc gctcaagagc gccgcgccct acatccgctt
      181 gtacaagggc aagaccttcg tggtgaaggc cggcggcggg gtgttcgccg cagaggactc
      241 cacccgctcg ctggtcgagc agatcgggat cctgcattac ttcggtgtgc gcgtggtgct
      301 ggtgcacggc ggcgggccgc agctcgccga gctgtcggcc gccctcggcg taccgacgca
      361 gatggtcgag ggccgccgcg tgaccgatca gcgctccatc gaggtcaccg ccatggtgct
      421 gaacggcctc atcaacaccc gcttgctcgc catgtgccgc gatctcaaca tcgacgcggt
      481 cggcatcagc ggcgtcgatg ccgggctggt gcgcgcccac aagcgcccgc ccgtgcgcct
      541 caccgccggt ggcgacccgg tggatttcgg ctacgtcggc gacatcgacg cggtcgacac
      601 cacggtgctc cgcaagctgc tcgacaacgg gctcatgccg gtggtgagcc cgctctcggc
      661 ggatgcgcgc ggcacgctcc tcaacatcaa cgccgacacc gtggccgcgg ccctcggcgc
      721 cgccctgggg gctgaaaagc tcatcctctg caccgctacg ccgggcattc tcggcagcgt
      781 cactgatccc ggctcactca tctcctacac ggacctcgcg ggattgaagc gcctgcgcga
      841 gtcgggccag atcgccgacg gcatgctgcc gaaggccaag gccatcgagg acgcgatccg
      901 cggcggcgtg cgccgcgtgc acgtcgtgtc gtaccgcgct cccgagggca tcctcggcga
      961 ggtgttcacc aacgagggca ccggaaccct catcgtcgcc gacatcaacg cgctctcccc
     1021 cgccgagcag caggggacgc tcccatgacg cgcctgtggg acaagggcgc gccgctcgac
     1081 gagcgggtgc tgcgctacac ggccggcgcc gactacgccc tcgatgaacg gctggtgcgc
     1141 tacgacgtgc gcggctcgat cgcgcacgcc gagatgctga atgcccggga gttgctgccg
     1201 ctcaccgacc tggatgcgat tcgcgatggc ctcgaggcgc tcgccgccga gcatgcccgc
     1261 ggcctctggc acgtggagct cgccgatgag gacgggcaga ccgcgctcga gcggcgcctc
     1321 accgagcgca tcggaccggc gggggcgcgc atccatctcg ggcgctcgcg caacgaccag
     1381 gtgctcacgg ccatccgcct gtacctgcgg gggacgaccg aggacctgga aagcggcgcg
     1441 ctccaggtgg cggaggcact ggacgagctc ggtgtgcgcg agggcggcac ggcgctcccc
     1501 ggctacacac acatgcagca ggccatgccg agctcggtgc cgctgtgggc cgggggcttc
     1561 gccgccgaga tccgcgacga cgcacagggc ctcgtcgagg tcgcgcgccg cctctcgcgc
     1621 agcccgctgg gatcggcggc cggctacggt acgccggggc tgcccat
//
LOCUS       DATLKL010000279         1861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_93368, whole genome shotgun sequence.
ACCESSION   DATLKL010000279 DATLKL010000000
VERSION     DATLKL010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93368"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..735
                     /locus_tag="VM713_03650"
     CDS             160..735
                     /locus_tag="VM713_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83393.1"
                     /translation="MFGRGPAKAMRSAAVVAALSACAAAGASSAATPAAESASWVQKE
                     VNFRYVGFTTKYSCDGLRDRMRGILLQLGARDDLKISGYGCTGVPAPETTPGVRVVMH
                     VLQPAGATPGQTTAAHWKTVDLLADRDLLDAARDCELISQLDRDVLPQFAVRHVDYRA
                     TCSVGTPLVGGTRLKADVLVADGASPAAASR"
     gene            790..>1861
                     /locus_tag="VM713_03655"
     CDS             790..>1861
                     /locus_tag="VM713_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HVH83394.1"
                     /translation="MRTRRNPGIAAIGVVLALMPVLSCGAPPSGGATTVGAGNARFEF
                     LTATLVRLEYSPSGAFVDAPTAVVEKRDWPQVRIDSREQDGWLIAASEAVTVRYRLRS
                     GAFTAANLEVRWKDRSGAERSWHPGDVDAGNLGGLNYSLDNIAAANLPKDGMDLESPV
                     DDSIPGIDLVLPKAQPGLLSRSGYALIDDSETPVWNAERRWIEPRRPTGGQDWYLFSY
                     GRDYHRVLEAYAELCGSIPMIPRYVLGPQITDFNFEYFPGSAQSRRPDFVRYNQRYLM
                     GEVARMRADRIPFDTLILDFAWHNYGWDGGYDWSPLFPQPLELMRALHARGVKLSLND
                     HPGYVHTDESILSFEDSHAPEVL"
BASE COUNT          301 a          627 c          628 g          305 t
ORIGIN      
        1 gatgccatgc cgcttaagcg gggccgctca accagttggg gcgcgggtgc aacagcgcgg
       61 caggacgcat ctggggcaag gggagtatcg tcgcagcaag gaacgtcagg cccggagcgc
      121 cgtccaaata gccggagcgg ctcaccagtg gaggctccca tgttcggcag aggtccggcg
      181 aaggccatgc ggagcgcggc ggtcgtggct gcgctgtcag cgtgtgcggc tgcaggcgca
      241 agctctgcgg ccaccccggc ggccgagtcc gcgagctggg tacagaaaga ggtcaacttc
      301 cgttacgtgg gatttaccac caagtacagc tgcgacggcc tgcgggacag gatgcggggc
      361 atcctgctgc agctcggcgc gcgtgacgac ctcaagatca gcggctacgg ctgcacggga
      421 gtccccgcac ccgagactac ccctggtgtc cgtgtcgtca tgcacgtgct ccagccggcg
      481 ggcgccacgc ccggccagac cacggcagcg cactggaaga ctgtcgatct gctggctgac
      541 cgggatctcc tcgatgcggc gcgcgattgt gagctgatct cgcagctcga ccgggacgtg
      601 ctgccacagt ttgcggtccg ccatgtcgac taccgcgcca cctgctccgt gggtacaccg
      661 ctggtcggag gcacgcggtt gaaggcggac gtcctcgttg ccgatggggc gtcgccggcc
      721 gctgcgagcc gctgagcctt ccgtacgccc cacgcgagtc ccgcaagacg ggaccgaagg
      781 gcactctgca tgcgcacccg gcggaatccc ggcattgcgg ccatcggcgt cgttctcgcc
      841 ctcatgccgg ttctgagctg cggggcaccg ccatcgggcg gtgccaccac ggtcggcgcc
      901 ggcaacgcgc gcttcgaatt cctgactgcg acactcgtgc ggctcgagta ctcgccgtcc
      961 ggcgccttcg tcgacgcgcc cacggcggtc gtcgagaagc gcgactggcc acaggtccgt
     1021 atcgattcgc gcgagcagga tggctggctc atcgccgcga gcgaggcggt gaccgtgcgc
     1081 taccggctgc gctcaggcgc cttcacagcc gcgaatctcg aggtgcggtg gaaggatcgc
     1141 tccggtgcag agcgcagctg gcaccccggg gatgtcgatg ccggcaatct cggcggactc
     1201 aactattcgc tcgataacat cgccgcggcg aacctgccga aggacgggat ggatctcgag
     1261 agcccggtcg atgattccat tccaggcatc gacctcgttc tgcccaaggc acagcccggg
     1321 ctcctgagtc gcagcggtta tgcgctcatc gatgacagcg agactcccgt gtggaacgcg
     1381 gagcgtcgct ggatcgagcc acggcgcccg accggcggcc aggactggta tctcttcagc
     1441 tacggccgcg attaccaccg ggtgctcgag gcgtacgcgg agctctgcgg atccattccg
     1501 atgatccctc gctacgtcct cggcccgcag atcaccgact tcaacttcga gtacttcccg
     1561 ggcagcgcgc agtcgcggcg gccggatttc gtccgctaca accagcggta tctgatgggc
     1621 gaggtggcgc ggatgcgcgc cgaccgcatt cccttcgaca cgctcattct cgatttcgcc
     1681 tggcacaact acggctggga cgggggctat gactggagcc cgctctttcc gcagccgctc
     1741 gagctgatgc gcgcgctgca cgcgcgcggc gtgaagctga gcctcaacga tcacccgggc
     1801 tacgtccaca cggacgagag catcctgtcg ttcgaggaca gtcacgcccc tgaagtgctt
     1861 g
//
LOCUS       DATLKL010000280         1895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_25657, whole genome shotgun sequence.
ACCESSION   DATLKL010000280 DATLKL010000000
VERSION     DATLKL010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25657"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..71)
                     /locus_tag="VM713_03660"
     CDS             complement(<1..71)
                     /locus_tag="VM713_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016138692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HVH83395.1"
                     /translation="MTFVVTENCIKCKYMDCVEVCPV"
     gene            complement(195..755)
                     /locus_tag="VM713_03665"
     CDS             complement(195..755)
                     /locus_tag="VM713_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVH83396.1"
                     /translation="MNYCSQCGAPVALRVPPGDHLPRHVCDACGTIHYQNPRLVVGCV
                     PEYEGRILLCRRAIEPRLGFWTVPAGFMENGETLQQAAARESHEEALARVEIGSLLAV
                     VHVLHARQVHVFFRAALRAPEFAPGAESLEVTLLAPADLPWSELAFPSTEFALRRYLE
                     DRAAGRESHHFTSIDRRLAQPGAGAT"
     gene            complement(805..>1895)
                     /locus_tag="VM713_03670"
     CDS             complement(805..>1895)
                     /locus_tag="VM713_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83397.1"
                     /translation="YHQRLHALDVATGAEQLNGPVEVAAIYPAAAGGSNTFAPANYEE
                     RAALLLVNGIIYTTWTSHCDIAPYSGWVIAYNSGTLAQSGVLNVAANAAGGGPSIWMA
                     GGGPAADSAGNIYVLTANGVFDTTLDANGFPNQGDYGNSFVKIASSGGALTVADYFTM
                     YNEVAESNGDVDLGSGGIMLLPDLADSGGTTRHLAVGAGKDGNLYVVSRDSMGKFNPA
                     DNSQIWQELSGALPGGIWSTPAYFNGTVYYGPVGAPLRAFKVMNAMLAGTPSSQSAAS
                     FAYPGTAAAISASGSANGIVWAHENGGSAVLHAYDASNLAHELYNSAQAAGGRDSFGA
                     GNKFITPTIADGKVFVGTTNGVAVFGLL"
BASE COUNT          291 a          627 c          682 g          295 t
ORIGIN      
        1 tccacggggc agacctcgac gcagtccatg tacttgcact tgatgcagtt ctcggtgacg
       61 acaaaggtca ttcaggttcc tcgaagcctg ggttgccggc ggcgcgcgca acgacggcgc
      121 ccgcggggcc acgtattgtg ccacggcgcc cggcgcaggt gaggatcgct ccggtctcag
      181 ctgcgacgca ggggttatgt ggcgcccgca cccggctgcg ccagccgccg atcgatgctc
      241 gtgaaatggt gggattcgcg gcccgcggca cggtcctcga gatagcggcg cagcgcgaac
      301 tccgtgctcg ggaatgccag ctcgctccac gggaggtcgg caggcgcgag cagcgtcact
      361 tcgagactct cggcgccggg tgcgaactcg ggcgcgcgca gcgcggcgcg gaagaacacg
      421 tgcacctgcc gcgcgtgcag cacgtgcacg accgcgagga gcgagccgat ttcgacacgc
      481 gccagcgcct cctcatgcga ctcgcgggcg gcggcctgct gcagcgtctc gccgttctcc
      541 atgaagccgg ccggcacggt ccagaagccg agccgcggct cgatggcgcg gcggcagagc
      601 agaatgcggc cctcgtactc gggcacacaa ccgacgacca ggcgcggatt ctggtagtgg
      661 atcgtgccgc aggcgtcgca tacgtggcgc ggcaggtgat cgcccggggg aacgcgcagc
      721 gcgaccggcg cgccgcactg gctgcaatag ttcatggcgg gcgccgagga tacgggcgcg
      781 cacctgggct tgtcgactcg gccctcacag caggccgaac accgccacgc cgttggtggt
      841 gccgacgaac accttgccgt cggcgatcgt cggcgtgatg aacttgttgc cggccccgaa
      901 ggaatcgcgg ccgcccgccg cctgagcgct gttgtagagc tcgtgcgcca ggttgctcgc
      961 gtcataggcg tgcagaaccg cggatccgcc gttctcatgt gcccagacga tgccgttggc
     1021 gctgccgctg gcggagatcg cagccgccgt gcccgggtag gcgaagctgg cggccgactg
     1081 cgatgagggc gtgccggcga gcatcgcgtt catcaccttg aaagcgcgca gcggcgcgcc
     1141 caccgggccg tagtacacgg tgccattgaa gtaggcgggg gtcgaccaga tgccgccggg
     1201 cagcgccccg gagagctcct gccagatctg actgttgtcg gcagggttga atttgcccat
     1261 cgagtcgcgg ctcaccacgt agagattgcc gtccttgccg gcgcccacgg cgaggtggcg
     1321 cgtcgtgccg ccggagtcgg cgaggtccgg gagcagcatg atgccgcccg agcccaagtc
     1381 gacgtcgcca ttcgactcgg cgacctcgtt gtacatggtg aagtagtcgg cgacggtgag
     1441 cgcgccgccg ctgctcgcga tcttcacgaa cgagttgccg tagtcacctt gattcgggaa
     1501 gccgttcgcg tcgagcgtcg tgtcgaacac gccattggcg gtgagcacgt agatgttgcc
     1561 ggccgagtcc gccgccggtc cgccgcccgc catccagatg gacggtccgc cgcccgcggc
     1621 gttggcggcg acgttgagca cgccactctg cgcgagcgtg cccgagttgt aggcgatcac
     1681 ccagcccgaa taaggcgcga tgtcgcagtg cgaggtccag gtggtgtaga tgatgccatt
     1741 cacgagcagc agtgcggcgc gctcctcata gttagccggc gcgaaggtgt tggagccgcc
     1801 cgccgcggcc ggatagatcg cggcgacctc cacgggcccg ttgagctgct cggcgcccgt
     1861 ggccacgtcg agcgcgtgca ggcgctgatg gtagt
//
LOCUS       DATLKL010000281         2242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_25670, whole genome shotgun sequence.
ACCESSION   DATLKL010000281 DATLKL010000000
VERSION     DATLKL010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25670"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2242)
                     /locus_tag="VM713_03675"
     CDS             complement(<1..>2242)
                     /locus_tag="VM713_03675"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HVH83398.1"
                     /translation="QIRASLDAVASLDDDRILRDYLNLVRAMLRTNYYQAGGDDRPKR
                     YLSFKLDPAQIPDLPLPRPKFEIFVYSPRVEGVHLRMGFVARGGIRWSDRREDFRTEV
                     LGLMKAQNVKNTVIVPVGAKGGFVPKRLAGTREEIQAEVVACYQTFIRGLLDLTDNIA
                     SGRIVPPRDVVRRDGDDPYLVVAADKGTATFSDIANAISADYGFWLGDAFASGGSAGY
                     DHKKMAITARGAWECVKRHFREMGVDTQKTDFTVAGIGDMSGDVFGNGMLLSRHIRLQ
                     AAFDHRHIFLDPDPDPAAAFAERARLFALPRSSWDDYDRKKLSRGGGVFPRSLKSIAL
                     PAEARALLGLEAASAAPNEIIRAVLRMPVDLLWNGGIGTYVKASDETNAEVSDRTNDG
                     LRINGRELRAKVVGEGGNLGLSQRGRVEYALKGGRLNTDFIDNSAGVNTSDVEVNIKI
                     LLNPQVAEGRLARGERNRLLARMTPAVADLVLRNNYLQGQAISTLEVQSAARLQEFQH
                     LIRSLERSGDLNRGLEFLPSDDELLERRKRGIGLARPELAILLAYSKIWLNNHLLASD
                     VPEDPYLSHELERYFPAPVQERFPRAIAHHRLRREIIATATTSSLVNRMGPTFVPRAQ
                     EDTGAEPAQIARAYTAAREIFAMREVWEQIEALDNRVPAKLQYEAAFQTSRLLRHATY
                     WLLSARRRDLQVDAAVKEFRSGVRELEARIAELLTGDELQRFSESRARYAQAGLPPQL
                     AARIASL"
BASE COUNT          326 a          735 c          812 g          369 t
ORIGIN      
        1 cgaggctggc gatgcgggcg gcgagctgtg gcggcagtcc cgcctgtgca tagcgagcgc
       61 gcgactcgct gaagcgttgc agctcgtctc cggtcaggag ctcggcgatg cgcgcctcga
      121 gctcgcgcac gccgctgcgg aattccttga ccgcggcatc gacctgcagg tcgcgccggc
      181 gcgcgctgag cagccagtag gtcgcgtgcc gcagcaggcg gctggtctgg aaggcagcct
      241 cgtactgcag cttcgcaggc acccggttgt cgagcgcctc gatctgctcc cacacctcgc
      301 gcatggcgaa gatctcgcgc gccgcggtgt aggcgcgggc gatctgcgcg ggctccgcac
      361 ccgtgtcttc ctgggcgcgc ggcacgaacg tcggtcccat gcggttcacc aggctgctgg
      421 tggtcgccgt ggcgatgatc tcgcggcgca accggtggtg agcgatggcg cgcgggaagc
      481 gctcctgcac cggcgcgggg aagtagcgct cgagctcgtg ggacaggtag gggtcctcgg
      541 gcacgtcgga ggcgagcagg tggttgttga gccagatctt gctgtaggcg agcaggatcg
      601 cgagctccgg ccgcgccagt ccgatgccgc gcttgcggcg ctcgagcagc tcatcgtcgc
      661 tcggcaggaa ctcgaggccg cggttcaggt cgccggagcg ctcgagcgag cggatgagat
      721 gctggaattc ctgcaggcgc gccgcggact gcacctcgag ggtgctgatc gcctggccct
      781 gcaggtagtt gttgcgcagg accaggtcgg ccaccgccgg cgtcatgcgt gccagcagcc
      841 ggttgcgctc gccgcgcgcg agtcgcccct cggcaacctg cgggttgagc aggatcttga
      901 tgttcacctc gacgtcggag gtgttgacgc cggcggagtt gtcgatgaaa tcggtgttga
      961 gccgtccacc cttgagggcg tactcgaccc ggccgcgctg cgagagcccg aggttgccac
     1021 cttcaccgac caccttggcg cgcagctccc ggccgttgat gcgcaggccg tcgttggtgc
     1081 ggtcgctgac ctcggcgttg gtttcatcgc tcgccttgac gtaggtgccg atgccgccgt
     1141 tccacagcag gtcgaccggc atgcgcagaa ccgcgcggat gatctcgttg ggcgcggcgc
     1201 tggcggcttc gagcccgagg agcgcgcgcg cctcggccgg cagggcgatc gacttcagcg
     1261 accgcgggaa cacaccgccg ccgcgcgaga gcttcttgcg gtcgtagtcg tcccagctcg
     1321 agcgcggcag cgcgaacagc cgcgcgcgct cggcgaaggc cgccgccgga tcgggatccg
     1381 ggtcgaggaa gatgtggcgg tgatcgaacg ccgcctgcag gcggatgtga cgcgacagca
     1441 gcatgccgtt gccgaacacg tcacccgaca tgtcgccgat gccggccacg gtgaagtcag
     1501 tcttctgcgt gtcgacgccc atctcgcgga aatggcgctt gacgcactcc caggcgccgc
     1561 gcgccgtgat tgccatcttc ttgtggtcgt agccggccga gccgccggag gcgaacgcat
     1621 cgccgagcca gaagccgtag tcggcggaaa tggcgttggc gatgtcggag aaggtggcgg
     1681 tgcccttgtc ggccgcgacc accaggtacg ggtcgtcgcc gtcgcggcgc acgacgtcgc
     1741 gcggcggcac gatgcgcccg gaggcaatgt tgtcggtgag atcgagaagt ccgcggatga
     1801 aggtctggta gcaggccact acctccgcct gaatctcctc gcgggttccc gcgagtcgct
     1861 tcggcacgaa gccacccttg gcgcccaccg gaacgatcac ggtgttcttg acgttctgcg
     1921 ccttcatgag accgaggact tcggtacgga agtcctcgcg ccggtccgac cagcggatgc
     1981 cgccgcgcgc cacgaacccc atgcgcaggt gcacgccctc gacgcgcggg ctgtagacga
     2041 agatctcgaa cttcggccgc ggcagcggca gatcgggaat ctgcgccggg tcgagcttga
     2101 aggacaggta gcgcttcggg cggtcgtcgc ctccggcctg gtagtagttg gtgcgcagca
     2161 tcgcccgcac caggttgaga tagtcgcgca ggatccgatc gtcatcgagg ctcgctaccg
     2221 catcgaggct cgcccggatc tg
//
LOCUS       DATLKL010000282         2612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_104655, whole genome shotgun sequence.
ACCESSION   DATLKL010000282 DATLKL010000000
VERSION     DATLKL010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104655"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1946
                     /locus_tag="VM713_03680"
     CDS             <1..1946
                     /locus_tag="VM713_03680"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HVH83399.1"
                     /translation="VIEDTPTPKDQLAARFGAVVAEIVDGVTKLDQIKFKSREEAQAE
                     SFRKMLLAMVRDLRVILVKLADRTHNMRTIEAMAPARRRAIARETLEIYAPIAERLGL
                     YNMKLELEDLGFKALYPRRYQVLERTLRRARGNQKEFLKKIEQQLKAALLKSDLQAEV
                     ETREKHLYSIYKKMHRKRSLLNEIVDVYGIRIILDRPDTCYRALGVVHSVFKPMPGRF
                     KDYIAIPRVNGYQSLHTTLFGPNGVPIEVQLRTADMHRVAESGIAAHWKYKAGDPSDS
                     AQQDRTREWLSNLVSLQEAGSSEEFLESVKVDLFPDKVYVFTPKGQILRLPSGATVVD
                     FAYAVHTDVGNRCVAAKVDRRLTPLRTVLRNGQTVEIITAKGAKPNPSWVNFVVTAKA
                     RSAIRHYLKGLRRAEAIALGSRLLSQALHEFGLALDDVKPEVKRAALGELGMKDLDEL
                     YEKLGLGERLAPLVARRLLPGAPAGDGKAAPAPLAIAGTEGLLVTYARCCFPIPYDPI
                     FAFLSTGRGVVIHRENCVNVEDYRKHPENWLPVSWQSAPDRLFSSEIRVHVVNRTGVL
                     AAVAAAIASTETNIDHVSIDEQDADAAVITFELRVRDRRQLARLVRVIRRMPDVTRVT
                     RSIAARARDEHGPESEAESDASG"
     gene            1981..2364
                     /locus_tag="VM713_03685"
     CDS             1981..2364
                     /locus_tag="VM713_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVH83400.1"
                     /translation="MSRQSIHTDRAPQAIGTYSQAVRAGDTVYLSGQVPFDPATMQLV
                     TGDIEVQARRVFENLKAVAEAAGGTLAHAVKLTIFLTDLANFAKVNEVMATYFAKPYP
                     ARSTIGVASLPRGAQIEIECVLHLA"
     gene            2418..>2612
                     /locus_tag="VM713_03690"
     CDS             2418..>2612
                     /locus_tag="VM713_03690"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83401.1"
                     /translation="MGAALAARLERLGVASVADLLFLLPLRYEDRTRVMAIGALAAGA
                     RATVEGEVQLTEVAYRRRRQL"
BASE COUNT          441 a          945 c          862 g          364 t
ORIGIN      
        1 atgtcatcga agacaccccg acgccgaaag accagctcgc ggcgcgcttc ggcgcggtgg
       61 tggcggagat cgtcgacggc gtcaccaagc tcgaccagat caagttcaag agccgcgagg
      121 aggcgcaggc ggagtcgttc cgcaagatgc tgctcgcgat ggtgcgcgac ctgcgcgtga
      181 tcctcgtcaa gctcgccgac cgcacccaca acatgcgcac catcgaggcc atggcgcccg
      241 cgcggcgccg ggcgatcgcg cgcgagacgc tcgagatcta cgcacccatc gccgagcggc
      301 tcggcctcta caacatgaag ctcgagctcg aggacctcgg cttcaaggcg ctctacccgc
      361 gccgctacca ggtgctcgag cgcacgctgc gccgcgcgcg cggcaatcag aaggagttcc
      421 tgaagaagat cgagcagcag ctgaaggccg cactcctgaa gagcgacctg caggcggaag
      481 tcgagacgcg cgagaagcac ctctacagca tctacaagaa gatgcatcgc aagcgctcgc
      541 tcctcaacga gatcgtcgac gtctacggca tccgcatcat cctcgaccgg cccgacacct
      601 gctaccgggc gctcggggtg gtgcactcgg tcttcaagcc catgcccggg cgcttcaagg
      661 actacatcgc catcccgcgg gtcaacggtt accagtcgct gcacacgacg ctgttcgggc
      721 ccaacggcgt gccgatcgag gtgcagctgc gcaccgccga catgcatcgc gtggccgaat
      781 ccggcatcgc ggcgcactgg aagtacaagg caggcgatcc gtccgactcc gcgcagcagg
      841 accgcacgcg cgagtggctc tcgaacctgg tgtcgctgca ggaggcgggc tcctccgagg
      901 agttcctcga gagcgtcaag gtcgacctgt tccccgacaa ggtgtacgtg ttcactccca
      961 aagggcagat cctgcgcctg ccctcgggcg ccacggtcgt ggacttcgcc tacgccgtgc
     1021 ataccgacgt cggcaaccgc tgcgtcgccg ccaaggtcga ccggcggctc acgccgctgc
     1081 gcaccgtgct gcgcaacgga cagacggtcg agatcatcac cgccaagggt gcgaaaccga
     1141 atccctcctg ggtcaacttc gtagtgaccg ccaaggcgcg cagcgccatc cgccactact
     1201 tgaaggggct gcggcgcgcc gaggcgatcg ccttgggctc gcggctcctg tcgcaggcgc
     1261 tgcacgagtt cggcctcgcg ctcgacgacg tgaagcccga ggtgaagcgc gctgcgctcg
     1321 gcgagctcgg catgaaggac ctcgatgagc tctacgagaa gctcgggctc ggcgagcgcc
     1381 tggcgccgct cgtagcgcgg cggctcctgc ccggggcgcc ggccggcgac ggcaaggcgg
     1441 cgccggcgcc gctcgcgatc gccgggaccg aggggctgct cgtgacctac gcgcgctgct
     1501 gcttcccgat cccctacgat ccgatcttcg ccttcctctc caccgggcgc ggcgtggtga
     1561 tccatcgcga gaactgcgtc aacgtcgagg actaccgcaa gcacccggag aactggctgc
     1621 cggtgagctg gcagagcgcg cccgatcggc tgttctcctc cgagatccgt gtccacgtcg
     1681 tcaaccgcac cggcgtgctg gcggcggtcg ccgcggcgat cgccagcacc gagaccaaca
     1741 tcgaccacgt atcgatcgac gagcaggacg ccgacgccgc ggtgatcacc ttcgagctgc
     1801 gggtgcgcga ccgccgccag ctggcgcgcc tggtgcgggt aatccgccgc atgccggacg
     1861 tcacccgcgt cacgcgctcc atcgccgcgc gggcgcgcga cgagcacggc cccgagagcg
     1921 aggccgagag cgacgccagc ggatagaaca acccacgaga ctctgaggag gagtgcgccg
     1981 atgagccgcc agagcatcca taccgaccgc gccccgcagg cgatcggcac ctactcacag
     2041 gccgtgcgtg ccggtgatac ggtgtatctc tcggggcagg tgccgttcga ccccgccacc
     2101 atgcagctcg tgaccgggga catcgaagtc caggcacggc gggtattcga gaacctgaag
     2161 gccgtcgccg aggccgccgg cggcacgctc gcccatgcgg tgaagctcac catcttcctc
     2221 accgacctcg caaacttcgc caaggtcaac gaggtcatgg cgacctactt cgccaaaccc
     2281 taccccgcac gctccaccat cggggtcgcc tccctgccgc gcggcgcgca gatcgagatc
     2341 gagtgcgtgc tgcacctcgc ttaagaccgc tgcccgccgc ggacgccggc gcgcgcccgg
     2401 tcacgagtct gcgcggcgtg ggagcggcgc tcgcggcacg cctcgagcgc ctcggcgtcg
     2461 cgagcgtggc cgatctgctg ttcctcctgc cgctgcgcta cgaggaccgc acgcgcgtca
     2521 tggccatcgg tgcgctcgcg gcgggggcgc gcgccacggt cgagggggag gtgcagctca
     2581 ccgaggtggc gtaccgccgc cgccgccagc tc
//
LOCUS       DATLKL010000283         1607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_25703, whole genome shotgun sequence.
ACCESSION   DATLKL010000283 DATLKL010000000
VERSION     DATLKL010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25703"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..>1607
                     /locus_tag="VM713_03695"
     CDS             154..>1607
                     /locus_tag="VM713_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HVH83402.1"
                     /translation="MALLVPTLTAVAAAPASEAPTATAGAKQSFPIVEFRILGNHVLE
                     RIKVEAAVYPFLGPERTLDTVQKAQAALVAAYHDAGFGTVLVDIPEQSVDDGVVRLKV
                     TEGRIGRVHIEGARYYSQRRILAALPAITPGTVPQLPQLQSELGQLAAEARDRAITPV
                     LKAGGAPGTMDVSLAVKDHLPLHGSLAVDNRYSADTAHTRLTGTLSYDNLFQRNETLS
                     LTYQVAPSDPSQVKVWVLSYLGHLPTPNWDWNAYAIRSESNVAAVGTLSVVGNGEVYA
                     AHLVHAFGGSASSVNSLNLGLDYKNFPQNVLLPGAVSSDTPIHYVLWSAQVATTRVHE
                     RYDLSGSLGAYFGLPGIAGDDTEFEFKRSGATPSFAYLRGSGSLTWRLWHGFALLGRT
                     EFQYTDSPLVSNEQFTLGGLDTVRGYLEAEELVDAGVAATLELHSPKLTLGRSETFAY
                     LFYERGVGMIQQPVPSEIDAGLVRSGLTSLGFGV"
BASE COUNT          262 a          537 c          525 g          283 t
ORIGIN      
        1 aaggggcaac ccttcctcct ctctcctttt cctgttcctg cgtttttctg ggccatcatt
       61 cgcaacgtga agagcacaat atcgccgcac accaccgcgg aaccgcgccc gccggtccga
      121 acgcggaacg gccgatgccg gttgtgcctc ggcgtggcgc tcctcgtccc aacgctgacg
      181 gcggtggcag ccgcgccggc ctcggaggcg ccgacagcga cggcaggagc gaagcagagc
      241 tttccgatcg tggagttccg gatcctcggc aatcatgtgc tcgagcgcat caaggtcgag
      301 gccgcggtct accctttcct gggtcccgag cgcaccctcg ataccgtgca gaaggcgcag
      361 gcggcactcg tcgctgcgta tcacgatgcc ggcttcggca ccgtgctggt cgacatcccg
      421 gaacagagcg tggacgatgg ggtggttcgg ctcaaggtga ccgaagggcg catcgggcga
      481 gtgcacatcg agggcgccag atactattcg caacggcgca ttctggcggc gctgccggcg
      541 atcacgcccg gcacggtgcc ccagctgccg cagctgcaga gtgagctcgg tcagctcgcc
      601 gccgaggcga gggatcgcgc gatcacgccg gtcctcaagg ccggcggtgc gccgggcacg
      661 atggacgtca gtcttgcggt gaaggatcac ttaccgctgc acgggtccct ggcagtcgac
      721 aaccgctact cggctgatac cgcccacacg cgcctgaccg ggactctcag ctacgacaat
      781 ctctttcagc gcaacgagac actctcgctc acctatcaag ttgcgccctc ggatccctcg
      841 caggtgaagg tctgggtgtt gagctacctc ggccacctgc ccaccccgaa ctgggactgg
      901 aacgcctacg cgatccgctc cgagagcaat gtcgcagcgg tcggcacgct atcggtagtt
      961 ggcaacggcg aggtttacgc cgcgcatctg gtgcacgcct tcggtggctc ggcgagcagc
     1021 gtgaacagcc tcaacctcgg gttggactac aagaactttc cgcagaatgt gctgctgccc
     1081 ggcgcggtca gttccgacac gcccatccac tacgtgctgt ggtcggcgca ggtcgcgacc
     1141 acgcgtgtgc acgagcgcta cgatctcagc ggcagcctgg gagcgtattt cggcctgccg
     1201 ggcatcgcgg gcgatgatac agagttcgag ttcaagcgtt ccggggcgac gccgagcttc
     1261 gcgtacctgc gtggctcggg cagtctcacc tggcggttgt ggcacgggtt cgcgctcctc
     1321 gggcgcacgg agttccagta cacagactcc ccgctggtaa gcaacgagca gttcaccctc
     1381 gggggtttgg atacggtgcg cggctatctc gaggccgagg agctggtcga cgcgggcgtg
     1441 gcggcaaccc tcgagctgca ctcccccaag ctcaccctgg ggcggtccga gactttcgcg
     1501 tacctgttct acgagcgcgg cgtcggcatg atccagcagc ccgtgccgag cgagattgat
     1561 gccggcctgg tgcgctcggg cctcacgagc ctcggcttcg gcgtgca
//
LOCUS       DATLKL010000284         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_161051, whole genome shotgun sequence.
ACCESSION   DATLKL010000284 DATLKL010000000
VERSION     DATLKL010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161051"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VM713_03700"
     CDS             complement(<1..299)
                     /locus_tag="VM713_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83403.1"
                     /translation="MTDDLVSAPWLRRVLTAAMLAGLLLLGFLVLDPFIVPVVWAAIL
                     AYVTWPGYEWLLGKCRGRGIIAALAMTTVVSAAVIVPLAWLAVVVRVELVHAYSET"
     gene            complement(296..772)
                     /locus_tag="VM713_03705"
     CDS             complement(296..772)
                     /locus_tag="VM713_03705"
                     /inference="COORDINATES: protein motif:HMM:NF023338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 family protein"
                     /protein_id="HVH83404.1"
                     /translation="MEPLGNDTAPARLGTAAELLGYLAAAPLIVCLACEATLPAYAGR
                     ELAQRGAIAWGAVLLASAGAVHWGLALAGRVPATAARLAAAAAPTPVAAAAVLIGGQR
                     ALALLVAGFGLFWLYEHRSLGSVLPPAYLGLRRNLSLATCTLLALTMIASDAAGLL"
     regulatory      839..1066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1175..>2032
                     /locus_tag="VM713_03710"
     CDS             1175..>2032
                     /locus_tag="VM713_03710"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVH83405.1"
                     /translation="MSQDLILRRALVCAAFALISCVGTAWGQQLPPSQQPGSSEQLEQ
                     VIVTASRVPEPADQSLFSVTVLTRADIEARQVLSVQDLLADLAGINIDSTGGLGQQSS
                     VFIRGADSDHTLLLIDGVRVGSATLGTAPFEIIPLEQIDHIEVVRGPRSTLYGSDAVG
                     GVIQIFTRQAGDPGTTLGGSAMTGSYDTHQFTGYLAARGREAWGSLSGDVLTTAGIPA
                     CLPSAVAGCFDPAAPQVPDGHQNRSGSLSAGVRLTDQLSASADVLYTHGWTAFDGAGF
                     DDRLDFEEKV"
BASE COUNT          314 a          707 c          743 g          268 t
ORIGIN      
        1 gtctcgctgt aggcgtggac cagctcgacg cgtaccacca cggcgagcca tgcgagcggc
       61 acgatgaccg ccgcgctcac cacggtggtc atcgcgagcg cggcgatgat gccgcggccg
      121 cggcacttgc cgagcagcca ctcgtagccc ggccaggtga cgtacgcgag gatcgccgcc
      181 cataccaccg gcacgatgaa gggatcgagc acgagaaaac cgagcagcag cagcccggcg
      241 agcattgccg cggtgagcac ccggcgcagc cagggcgcgg agaccagatc gtccgtcaca
      301 gcagccccgc ggcgtcggag gcgatcatgg tgagcgccag cagcgtgcag gttgcgaggc
      361 tcaggttgcg ccgcaggccg aggtatgcgg gtggcagcac gctgccgagc gagcggtgct
      421 cgtagagcca gaagagtccg aagcctgcca ccagcagcgc gagcgcgcgc tgaccgccga
      481 tcaggaccgc cgccgccgcg accggggtgg gcgcggccgc cgccgcgagg cgggccgcgg
      541 tcgcaggcac gcggccggcg agcgcgagtc cccagtgcac cgcgccggca gaggcgagca
      601 gcaccgcccc ccaggcgatc gcgccgcgct gcgcgagctc gcgcccggcg taggcgggga
      661 gggtcgcctc gcaggcgaga cacacgatga gtggcgccgc cgccagatag ccgagcagct
      721 cggccgcggt gccgagccgg gcgggtgcgg tgtcgttccc cagcggttcc acggcccgcg
      781 atgatgccac gcggggcggc gtgttgaatt gtcttgacgc cggataccgc gtacaggcag
      841 gcttcgcgcg gcgtgaggtg ctcgttcccg tgagggaacg ggtaacacgg gaagtccggt
      901 gcggattcgt ccaaacccgg cgctgccccc gcaacggtaa tcgagatcgg gcggatcaag
      961 agccactgca cctgagtcaa gaagagggtg cgggaaggcg atccacccgg aggcttaagg
     1021 cccccactcg agagcccgga gaccggcctc gcgcatcacc gttggacgtc gcggcgggcg
     1081 acgctggtcg aacggtgtgc gcctcagggc gcagacctgc cagctcctga cacgacccaa
     1141 cgggcacggg tgtgtgctgt cgaggagctc tgccatgtct caggatctca tcttgcgccg
     1201 cgcgctggtc tgcgcggctt ttgcgttgat ctcgtgcgtg gggacggcgt ggggccagca
     1261 gttgccgccg tcgcagcagc cgggatcgag cgagcagctc gagcaggtca tcgtcaccgc
     1321 ctcgcgcgtg ccggagcccg cggaccagtc gctgttctcg gtgacggtgc tgacgcgcgc
     1381 cgacatcgag gcgcgccagg tgctgtcggt gcaggatctg ctggcggatc tggccggcat
     1441 caacatcgac agcaccgggg ggctcggcca gcagagctcg gtattcatcc gcggcgccga
     1501 ctccgaccac acgctgctgc tcatcgacgg cgtgcgcgtc ggctcggcga cgctcggcac
     1561 cgcgcccttc gagatcatcc cgctcgagca gatcgatcac atcgaggtcg tgcgcggacc
     1621 gcgctcgacg ctctacggct cggacgcggt cggcggggtc atccagatct tcacccgcca
     1681 ggcgggcgac cccgggacga ccctcggcgg cagcgccatg accggcagct acgacacgca
     1741 ccagttcacg ggctacctgg cggcgcgcgg ccgcgaggcc tggggcagcc tctcgggcga
     1801 cgtgctgacc accgccggca tccccgcctg cctgccctcg gcggtggccg gctgcttcga
     1861 cccggccgcg ccgcaggttc cggatggcca tcagaaccgc tccggctcgc tgagtgccgg
     1921 cgtgcggctc accgatcagc tcagcgcatc ggccgacgtg ctctacaccc atggctggac
     1981 ggcgttcgac ggcgccgggt tcgatgaccg gctggatttc gaggagaagg ta
//
LOCUS       DATLKL010000285         1809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_206112, whole genome shotgun sequence.
ACCESSION   DATLKL010000285 DATLKL010000000
VERSION     DATLKL010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206112"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VM713_03715"
     CDS             <1..365
                     /locus_tag="VM713_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HVH83406.1"
                     /translation="HFATAAEVARRLVAQLLRKPLEAALILNVNVPDVPLDALRGFRA
                     TRLGYRHRSEPVMRAEDPRGRPVYWVGPAGAEQEAGPGTDFDAVARGYVSVTPLHVDL
                     TRHGALPGVSTWLEGADG"
     gene            358..1035
                     /locus_tag="VM713_03720"
     CDS             358..1035
                     /locus_tag="VM713_03720"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003222172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HVH83407.1"
                     /translation="MADVRLTGIGMTSARTRDRLVQRLREQGIANLAVLDRIRNVPRH
                     IFVDEALGSRAYEDTALPIGFGQTISQPYIVARMTEALLAGGAAWAGTLDNVLEVGTG
                     CGYQTAVLAPLVTQLYTIERIEPLLARARERLKELGIRNVRFRHGDGSLGWKAHAPYD
                     GILVAAAPLTVPEALLQQLKIGGRLIVPLGPEGEQELVRFTRREPRIERESLGAVAFV
                     PLLGGTA"
     gene            complement(1427..>1809)
                     /locus_tag="VM713_03725"
     CDS             complement(1427..>1809)
                     /locus_tag="VM713_03725"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HVH83408.1"
                     /translation="RTLVKFTLEPGAPVNLIRGYSDSEGRIGEHAVRGSCIVTADTLI
                     TQWEPRSFAELRPAHLEAVLALSPELIVLATGPAQQFPPAEIRALLARRGVGLEAMQL
                     GAACRTFNVLVQEERRVAAALFLR"
BASE COUNT          294 a          607 c          607 g          301 t
ORIGIN      
        1 gacatttcgc gacggctgcc gaggttgcgc gccgcctggt cgctcagctc ctgagaaagc
       61 cgctcgaggc ggcgctcatt ctcaacgtca acgtgccgga cgtgccgctc gatgccctgc
      121 gcggcttccg cgcgacccgc cttggctatc ggcaccgctc cgagccggtc atgcgggccg
      181 aggatccgcg cgggcggccg gtgtactggg tggggccggc cggagccgag caggaggccg
      241 gtcccggcac ggacttcgat gcggtcgcgc gcggctacgt gtcggtgacg ccgctgcatg
      301 tcgatctcac gcgccacggc gcgctgcccg gggtgtccac ctggctcgag ggcgccgatg
      361 gctgacgtgc gcctcaccgg tatcggcatg acctcggcgc gcacgcgcga tcgcctggtg
      421 cagcggctgc gcgagcaggg catcgccaac ctcgccgtgc tcgatcggat ccgcaacgtg
      481 ccgcgccaca tcttcgtcga tgaggcactc ggcagccgcg cctacgagga cacggcgctg
      541 ccgatcggct tcggtcagac gatctcccag ccctacatcg tggcgcggat gaccgaagcg
      601 ctgctcgcag gaggagcggc ttgggcgggc acgctcgaca acgtgctcga ggtcggcacc
      661 ggttgcggct accagaccgc cgtgctcgcg ccgctcgtga cgcagctcta tacgatcgag
      721 cgcatcgagc cgctgctcgc gcgggcacgc gagcggctga aggagctcgg catcaggaac
      781 gtgcgcttcc ggcacggcga cggctctctc ggttggaagg cgcacgcgcc ttacgacggg
      841 atcctggtgg ccgccgcccc gctaaccgta ccggaggcgc tgctgcagca gctgaagatc
      901 ggcggtcgcc tgatcgtccc cctgggtccc gagggagagc aggagctcgt gcgcttcacc
      961 cgccgtgagc cgcgcatcga gcgggagtcc ctgggtgccg tcgccttcgt gccgctgctc
     1021 ggcggtactg catgattttc agtcgagata tatgccgtaa ctcattgttt atagatacat
     1081 caattcactc acaaagaggc gaaattgccg ctccgactcg caaatttcag cgtcaaaggc
     1141 gcctcgcggg ccacgcagac cgcgcaatcg tggggaaaag tccttgcgtg tcaaggcgct
     1201 tgcgcgggta tctacgacgt gtttaccatg cgcaccctgt ctctcgacga cgacaggtca
     1261 ccggggaggg ccatcagcag aaggcacagg gaggagggag tcgcgacaac agcaataacg
     1321 ttcccaacaa caacaagaac agtgcaattg ttcgagtctg tcagcttcga atgattgttt
     1381 ggttggttga gcctccccct gtgtcttctg ccgatcgtct tcccgattag cgcaaaaaca
     1441 gcgccgcggc cacccgccgc tcctcctgca ccagcacatt gaacgtccgg cacgcggcgc
     1501 cgagctgcat cgcttcgagc ccgacgcccc gtcgcgcgag cagcgcgcgg atctcggccg
     1561 gcggaaactg ctgagcgggg ccggtggcga gcacgatgag ctcggggctg agcgcgagca
     1621 ccgcctcgag gtgcgcgggc cgaagctccg cgaagctgcg cggctcccac tgtgtgatca
     1681 gggtgtcggc ggtaacgatg cagctgccgc gcaccgcgtg ctcgccgata cgcccctcgc
     1741 tgtccgagta gccgcggatc agattgaccg gtgccccggg ttcgagcgtg aacttcacga
     1801 gcgtacgat
//
LOCUS       DATLKL010000286         1805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_194842, whole genome shotgun sequence.
ACCESSION   DATLKL010000286 DATLKL010000000
VERSION     DATLKL010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194842"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VM713_03730"
     CDS             <1..1202
                     /locus_tag="VM713_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HVH83409.1"
                     /translation="SFITWASLVGVCVGVAALIVILSVMNGFESELRDRLLSLGAQLR
                     VIAAAAPGGPTPISGALDWQDAARRARAVAGVVAAAPYAELTALAVREPEMMPVVLRG
                     IEPQQSAAVTDLGKAITQGRLTDLVPGSGRVIVGELIAERLGLAVGEQLTLLVPTVSA
                     DGTPEARLRGFTVAGVFEVGLQEHDGTLVFAPLEDVRALTPASASGQALSVRIADVLA
                     APSVAARLRVALPKVYEVRDWTEDNASYFRATRIEKTMMSLILLLIVAVAAFNIVAML
                     VMVVTDKRTDIAILRTFGAVPRRVMGIFITQGLVIGWLGVALGVALGLTLALNVETIE
                     PFLERTLHFQILDSDVYYSSVIPSEVHATNVVAIGAAALLLTALATIYPAIRAARTAP
                     AEALRYE"
     gene            1205..>1805
                     /locus_tag="VM713_03735"
     CDS             1205..>1805
                     /locus_tag="VM713_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVH83410.1"
                     /translation="MAGAVYEWLIGTRYLRSTHRRGFVSFVALMSVCGLTLGVATLLV
                     VLSVMNGFERELRTRILAVTSHATLTGLSGTLADWRTLAQRARGQSGVVAAVPYVESE
                     AMLANGYALAGATVRGVLPDEERTATGLAQRLTAGKLSDLKPGGYGIILGSALAHELG
                     ARMGGTVVVIAPQGTATPTGVVPRMRRFRVVGLFQSGMYE"
BASE COUNT          231 a          625 c          657 g          292 t
ORIGIN      
        1 tgtcgttcat cacctgggcg tcgctggtgg gagtgtgcgt cggggttgcg gcgctcatcg
       61 tcatcctctc ggtgatgaat ggcttcgaga gcgagctacg cgaccggctg ctgtccctcg
      121 gcgcccagct acgcgtgata gcggcggccg cgccgggtgg gccgacgcca atctccggcg
      181 cgctcgactg gcaggacgcg gcgcggcgtg cgcgcgccgt cgccggcgtc gtggccgcgg
      241 ctccctacgc ggagctcacc gcgctggcgg tgcgcgagcc cgagatgatg ccggtcgtgc
      301 tgcgcggcat cgagccgcag cagagcgccg cggtgaccga tctcggcaag gcgatcactc
      361 aggggcgcct cacggacctc gtgccgggct ccgggcgcgt catcgtcggc gagctgatcg
      421 ccgagcggct gggccttgcg gtgggcgagc agctcacttt gctggtgcct accgtgagcg
      481 cggacggcac gcccgaggcc aggctgcgcg gcttcaccgt ggcgggcgtc ttcgaagtgg
      541 ggctgcagga acacgacggc acgctggtat tcgcgccgct cgaggacgtg cgcgccctca
      601 ccccggccag cgccagcggg caggcgctca gcgtccgcat cgccgacgtg ctggccgcgc
      661 cgtcggtggc ggcgcggctg cgcgtcgcct taccgaaggt ctacgaagtg cgcgactgga
      721 ccgaggacaa cgccagctac tttcgcgcga cccgcattga gaagaccatg atgtcgctca
      781 tcctgctgct gatcgtcgcg gtcgccgcct tcaacatcgt ggcgatgctg gtgatggtgg
      841 tcaccgacaa gcgcaccgac atcgcgatcc tgcgcacctt cggcgccgtg ccgcgccggg
      901 tgatgggcat cttcatcacg cagggactgg tgatcggctg gctcggggtg gcgctcggcg
      961 tggcgctcgg cctcacgctg gcgctcaacg tcgagaccat cgagccgttc ctcgagcgca
     1021 cgttgcactt ccagatcctc gactcggacg tctactacag cagtgtcatc ccctccgagg
     1081 tgcacgccac gaacgtggtg gcgatcggcg cggcggcgct gctgctcacc gcgctcgcga
     1141 cgatctaccc ggcgatccgc gcggcccgca cggcgcccgc cgaagcgttg cgctacgagt
     1201 agccatggcg ggcgccgttt acgagtggct gatcggcacg cgctacctgc gctcgacgca
     1261 ccggcgcggc ttcgtatcgt tcgtggcgct catgtcggtg tgtggactga cgctcggggt
     1321 ggcgaccctc ctcgtggtgc tgtcggtcat gaacggcttc gagcgggagc tgcgtacccg
     1381 cattctcgcc gtgacctcgc acgccacgct caccgggctg tccggcaccc tcgccgactg
     1441 gcgcacgctc gcgcagcgcg cccggggcca gagcggggta gtggcggcgg tgccgtacgt
     1501 cgaaagcgag gcgatgctcg ccaacggcta cgcgctggcc ggcgccaccg tgcgcggcgt
     1561 gcttccggac gaggagcgca ccgcaaccgg gctggcgcag cgcctgaccg ccggcaagct
     1621 gagcgatctc aagcccggcg gctacggcat catcctcggc tcggcgctgg cgcatgagct
     1681 cggcgcgcgc atgggcggta cggtggtggt gatcgcgccg cagggtaccg caacgcccac
     1741 cggcgtcgtg ccgcgcatgc gccgcttcag ggtggtgggc ctgtttcagt cgggcatgta
     1801 cgagt
//
LOCUS       DATLKL010000287         2015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_48180, whole genome shotgun sequence.
ACCESSION   DATLKL010000287 DATLKL010000000
VERSION     DATLKL010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48180"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1370)
                     /gene="gorA"
                     /locus_tag="VM713_03740"
     CDS             complement(18..1370)
                     /gene="gorA"
                     /locus_tag="VM713_03740"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF004776.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HVH83411.1"
                     /translation="MGEQFDLVVIGGGSGGLAAAQRAAEYGARAVLIESGRLGGTCVN
                     VGCIPKKIMWSAADLGGALHDAREYGFEVAAAGHDWRSLKRKRDAYITKLNDIYAANL
                     ARRNVELLRGRASFLDARTVRAAGRTLSAPHIVIATGGRPEVPPVPGAELGITSDGFF
                     ELEALPPRVLVAGGGYIAVELSGIFAGLGAQTTLALRGPAPLRTFDPMIAETSLAMLR
                     EAGVTVVTDIAPGTVARDAGGALIATFADGRSIGPFDRLLWAVGRQASVADLALERAG
                     VRLDADGFVATDMYQATSAAGIYAVGDVAGRAQLTPVAIAAGRRLADRLFGGQAGRHL
                     DYENIPTVIFGRPPIGTVGLTEPAARARYGRDAVTVFRSSFVPLYHALTEAQPRTAMK
                     LVTVGAEQRIVGLHVVGAGADEMLQGFAVAVRMGATKKDFDDTVAIHPTSAEELVTMR
                     "
     gene            1450..>2015
                     /locus_tag="VM713_03745"
     CDS             1450..>2015
                     /locus_tag="VM713_03745"
                     /inference="COORDINATES: protein motif:HMM:NF040252.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83412.1"
                     /translation="MDNPLLGQDPLPRFVQIRPEHVEPAVRVLLERNRARIGELAALA
                     RPTFATLIEPLEELQHGITRTWSPVSHLNAVLNSDALRAGYNACLPLLSAYHTDLAQS
                     EPLFRAYRTIAERDAATLAPVQRRVIEHAVRDFRLAGVALPAEQKSRFKTVMQELTQL
                     QVKFEENLLDAANAWSWHVTDPDELRGL"
BASE COUNT          302 a          743 c          684 g          286 t
ORIGIN      
        1 gagcgatccg cgagtcgcta gcgcatcgtg accagttcct ccgcgctggt cggatggatc
       61 gccacggtgt cgtcgaagtc cttcttggtg gcgcccatgc gcacggccac ggcgaatccc
      121 tgcagcatct cgtcggcccc cgcccccacg acgtgcagcc ccacgatgcg ctgctcggcg
      181 ccgaccgtca ccagcttcat cgcggtgcgc ggctgcgcct ccgtcagcgc gtgatagagc
      241 ggcacgaagc tcgagcggaa caccgtgacg gcgtcgcggc cgtagcgcgc ccgggccgcg
      301 ggctcggtca gccccacggt gccgatcggc ggacgcccaa agatcaccgt cgggatgttc
      361 tcgtagtcga gatgccggcc cgcctggccg ccgaagaggc gatcggcgag acgccttccg
      421 gcggcgatcg ccaccggggt gagttgcgct ctacctgcga cgtcgccgac ggcgtagata
      481 cccgcggcgc tcgtcgcctg gtacatgtcg gtagccacga agccgtccgc atccagcctc
      541 acaccggcgc gctcgagggc aaggtctgcg accgaggcct gccttcccac ggcccacagc
      601 aggcggtcga agggcccgat gctgcggccg tcggcgaagg tagcgatgag cgcgccgccg
      661 gcgtcgcgcg caaccgtgcc gggcgcgatg tcggtcacca ccgttacgcc cgcctcccgc
      721 agcatcgcga ggctcgtctc ggcgatcatc gggtcgaagg tgcgcagcgg cgcgggaccg
      781 cgtagtgcca gcgtcgtctg cgcgcccagg cccgcgaaga tcccgctgag ctcgaccgcg
      841 atatagccgc cgcccgcgac gaggacgcgc ggcggcaaag cctcgagctc gaagaagccg
      901 tcggaggtga tgccgagctc cgcccccggc acgggcggca cctcgggacg cccgccggtc
      961 gcgatgacga tgtgcggcgc gctcaaggtg cgcccggcgg cacgcacggt gcgcgcgtcg
     1021 agaaagctcg cccggccgcg cagcagctcg acgttgcggc gggcgaggtt cgcggcatag
     1081 atgtcgttga gcttagtgat ataggcgtcg cgcttgcgct tcagcgagcg ccagtcgtgg
     1141 cccgccgcgg cgacctcgaa gccgtactcg cgcgcatcgt gcagcgcgcc gccgaggtcc
     1201 gccgcgctcc acatgatctt cttgggtatg cagcccacgt tcacgcacgt accgccgagg
     1261 cggcctgact cgatgagcac ggcgcgggcg ccgtattcgg ctgcgcgttg cgccgccgcg
     1321 agcccgccgc tgccgccgcc gatcaccacc agatcgaatt gctcgcccat agacacctcg
     1381 gaccgccatg ttatatgagg ggccggcggc gcggtcctgc cggcgcagtc ggctacaata
     1441 gtgccccgga tggacaaccc cttactcggc caggatccgc tgccgcgctt cgtgcagatc
     1501 cgccccgagc acgtggagcc ggcggtgcgt gtgctcctcg agcgcaaccg cgcccgcatc
     1561 ggtgagctcg cggcgctcgc gcgacccacc ttcgcgacgc tgatcgagcc gctcgaagag
     1621 ctgcagcacg gcatcacgcg tacctggtcg cccgtgagcc acttgaacgc ggtgctcaat
     1681 tccgacgcgc tgcgcgccgg ctacaacgcc tgtctgccgc tcctgtccgc ctaccacacg
     1741 gatctcgcgc aaagcgagcc gctgttccgc gcctatcgca cgattgccga gcgcgacgcc
     1801 gccacactcg cgccggtgca gcgccgggtc atcgagcacg ccgtgcggga cttccgcctc
     1861 gccggcgtcg ccctgccggc cgagcagaag agccgattca agaccgtgat gcaggagctc
     1921 acgcagctgc aggtgaaatt cgaggagaac ttgctggacg cggccaacgc gtggagctgg
     1981 cacgtcaccg acccggacga gctgcgcggc ctcaa
//
LOCUS       DATLKL010000288         4543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_104720, whole genome shotgun sequence.
ACCESSION   DATLKL010000288 DATLKL010000000
VERSION     DATLKL010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104720"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2469)
                     /locus_tag="VM713_03750"
     CDS             complement(<1..2469)
                     /locus_tag="VM713_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVH83413.1"
                     /translation="MSALRAASLRHLLRHPAQLALALIGLTLGVGTIVAVDVATASAR
                     RAFELSVAAVNGAATHQIVGGPAGIDEQLYVRIRHAMRPDAAPGPRFAPIVAGYVSAG
                     GRIMQLIGIDPFASAELEGGAGRPGALGVQGLEEARGWFTRPGAVVMSAAAARELGVL
                     EGGTFELSVGGVPHRATLIARLTASQAGLDTVILTGLAQAQEWLGSIGRLSRIDVGLP
                     AAGTGAALAGGLAALLPAGLMLRETSTEARETLAMTDAFTTNLRAMSLLALLVGTLLI
                     YGAVSFAVLQRRAIIGVLRALGATRREVLTMVLGEAAALGLAGALCGVALGVLIGRGL
                     VGLVSRTINDLYFVVAVNEVSVPPGALLEALGAGIATALVAALLPALEVAASTPQLGL
                     ARSVLEGRAARAARSLALLGLGLACAAGLTIALSSRSLFAGFVALFLLLLAAAAVTPA
                     MLRVLAAAASRLSAGTSSVARLAWADVGASLSRTGVAIAALGMALTAMIGVGVMVESF
                     RESLREWLVQTMRADVYVTAPGVTEGLERRLEPEVIRAILAVPGIRDHSEGRRVTVAS
                     PVGPVDLNAVRLAPSSYSGFRFTAGDRAAAWREFAQGAILISEPLAWRLSLAPGSSLT
                     LITAAGAHDFAVAGVYREYGNDRGEVLIDLGEYRRWWHEEGIAGLGLYLGPGVSDAQL
                     IGAVRAAASTRQALLVRSNAAIRELSMRIFERTFVITRVLYWLAAGVAAVGLVSALLA
                     WELERARELALLRTLGLTPAATAALVLGQTLFMGLVALLAAIPAGLLTALILTEVINR
                     RAFGWRIDLHLTAAQFSNALWLALA"
     gene            complement(2466..3218)
                     /locus_tag="VM713_03755"
     CDS             complement(2466..3218)
                     /locus_tag="VM713_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVH83414.1"
                     /translation="MVDGDHPVNSTMQPQEPTRVLRIRQLSRSFHEGAHVHRALDGLE
                     AEVRPGERVAIMGRSGSGKSTLLNLISGIDRADSGTIEMDGVEITALAEPARTLYRRA
                     HVGFVYQFFNLIATLDVAENVRLVLELNGVRGARAQARSLAALEAVGLAARAHSAVDQ
                     LSGGEQQRVAIARALVHEPQLLLADEPTGNLDEETAREVLPVLLSLTRARGATLLMVT
                     HDEALAATADRVLELREGRLLERHAPPGGQSR"
     gene            3201..3866
                     /locus_tag="VM713_03760"
     CDS             3201..3866
                     /locus_tag="VM713_03760"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HVH83415.1"
                     /translation="MITVHHLNNSRSQRVLWLLEELGCEYSLVKYQRDPHTLLAPPEL
                     RRIHPLGKSPVITDGAHTLAESGAILEYLVDRYGDGRLRPPPGSAEQLRYTYWLHYAE
                     GSAMPPLLLKLVFLRLPRPVPALIRPIVRAVAQRAQQSFIDPQLKTHIDYWEAELGKS
                     TWFAGEELTAADVQMSFPLEAAAARAGIALGPRVTQFLARIHARPAYRRALERGGEFR
                     LAR"
     gene            4057..>4543
                     /locus_tag="VM713_03765"
     CDS             4057..>4543
                     /locus_tag="VM713_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HVH83416.1"
                     /translation="MTLPARHRGRITLTLGLAAAAVLLVVSLPATAQQPATPAAATPA
                     AATPATAPAAAKKAKAPATSAAAESPASARSYSLGLMWGDQLRTTGVAPDGINAARIA
                     QGVHDAITGKVALTDKDHENIRALANSAGDSNHQVAAKFLAENGKKPGVVTTASGLQY
                     QV"
BASE COUNT          722 a         1684 c         1571 g          566 t
ORIGIN      
        1 cgcaagcgcg agccacagcg cgttgctgaa ctgtgccgcg gtgagatgca gatcgatccg
       61 ccagccgaag gcgcggcgat tgatcacctc cgtcagaatg agagccgtga ggagacctgc
      121 ggggatcgcg gcgagcaatg ccaccagccc catgaacagc gtctgcccga gcaccagcgc
      181 cgcggtggcc gcgggggtga gccccagggt gcgcagcagc gcgagctcgc gcgcgcgctc
      241 gagctcccag gcgagcagcg cactcacgag gcccaccgcg gcgacgccgg cggccagcca
      301 gtagagcacc cgggtgatga cgaaggtccg ctcgaagatg cgcatcgaga gctcgcggat
      361 cgccgcattc gagcgcacca gcagcgcctg gcgtgtgctg gccgcggcgc gcaccgcgcc
      421 gatcagctgc gcgtcgctga cgccggggcc gagatacagg ccgagtccgg caatgccctc
      481 ctcgtgccac cagcgccggt actcaccgag atcgatcagg acttcgccgc ggtcgttgcc
      541 atactcgcgg tagacgcccg cgaccgcgaa gtcgtgcgca ccggcggccg tgatgagcgt
      601 gagcgagctg ccaggggcca gcgacagccg ccaggcgagc ggctcggaaa tgagaatcgc
      661 tccctgcgca aactccctcc aggcagcggc tcgatcgccg gccgtgaagc ggaagcctga
      721 gtaactcgag ggagcgagcc tcacggcgtt gaggtccacc gggccgacgg gcgaggcgac
      781 cgtgacccgc cgtccctcgc tgtggtcgcg gatacccggc accgcgagga tcgcccggat
      841 gacctcgggc tcgagccgcc gctcgaggcc ctcggtcacg ccgggggccg tcacgtaaac
      901 gtccgcccgc atcgtctgca cgagccactc ccgcagcgac tcgcgaaagc tctcgaccat
      961 gacgccgacg ccgatcatcg ccgtgagcgc catgccaagc gcggcgatcg caaccccggt
     1021 acggctcagc gaggcaccca cgtccgccca ggcgagccgc gccacggagc tcgtgcccgc
     1081 actgagcctt gaggcggccg cggcaagcac gcgcagcatc gcaggcgtca ccgccgccgc
     1141 cgcgagcagc agcaggaaga gcgccacgaa gcctgcaaag aggctgcggc tcgagagcgc
     1201 aatcgtgagt cccgcagcgc atgccagccc gaggccgagg agcgccaggc tgcgcgcggc
     1261 acgggcggcg cgtccctcga gcaccgagcg cgcgagcccg agctgtgggg tgctggccgc
     1321 cacctcgagc gcggggagca gcgccgccac gagcgcagtt gcgataccgg cccccagggc
     1381 ctcgagcagc gcgcccggcg gcacgctcac ctcgttcacc gccaccacga agtagagatc
     1441 gttgatggtc cgcgacacga gtcccacgag ccccctgccg atgaggaccc cgagggccac
     1501 gccgcacagg gcgccggcga gcccgagcgc cgccgcctcg cccagcacca tcgtgagcac
     1561 ttcgcgccgc gtcgcgccga gcgcgcgcag cacgccgatg atggcgcgcc gctgcagcac
     1621 ggcgaagctc accgcaccgt agatgagcag tgtccctacg agcagcgcga gcaggctcat
     1681 cgccctgagg ttggtcgtga acgcatccgt catcgccagg gtctcgcgcg cctccgtgct
     1741 cgtttcgcgc agcatgagcc ctgcgggcag cagcgcggcg agtccgcccg ccagggcggc
     1801 gcccgtgccg gcagcgggca ggcctacgtc gatccgcgac aggcggccga tcgacccgag
     1861 ccactcctgt gcctgcgcga ggcccgtgag tatcacggtg tcgagaccgg cttgggaggc
     1921 ggtgaggcgc gcgatgagcg tggcgcggtg cggcaccccg ccgacgctga gctcgaaggt
     1981 gccgccctcc aggacgccca gctcgcgcgc ggcggctgca ctcatgacga ccgcgccggg
     2041 gcgagtgaac cagccgcgcg cctcctccag accctgcaca ccgagggccc ccggccgccc
     2101 tgccccgccc tcgagctcgg cgctcgcgaa cggatcgatg ccgatgagct gcatgatgcg
     2161 cccgcccgcg ctcacgtagc cggcgacgat gggcgcaaac cgcggacctg gcgcggcatc
     2221 ggggcgcatg gcgtggcgga tccgcacgta cagctgctcg tcgatgccgg cggggccacc
     2281 gacgatctga tgggtggcgg ctccgttcac cgcagccacc gagagctcga aggcgcgccg
     2341 cgcgctcgcc gtcgccacgt caaccgccac gatggtgccg accccgagcg tgagcccgat
     2401 gagcgccagc gccagttgcg ccgggtggcg cagcaggtgc cgcaggcttg ccgcgcgcag
     2461 tgcgctcacc ggctctgacc gccgggcggt gcgtgccgct cgagcagccg cccctcacgc
     2521 agctcgagca cgcggtcggc ggtggcggcg agcgcctcat cgtgcgtcac catcaggagc
     2581 gtcgcgcccc gcgcccgcgt cagcgacagc agcaccggca acacctcgcg ggcggtctcc
     2641 tcgtcgaggt tgccggtcgg ctcatcggcc agcagcagtt gcggctcgtg caccagggcg
     2701 cgcgcgatgg ccacgcgctg ctgctctccg ccggagagct gatcgaccgc actgtgcgcg
     2761 cgggcggcga gacccacggc ctcgagcgcg gccaggctgc gcgcctgcgc ccgcgcgccg
     2821 cgcactccgt tgagctcgag caccaggcgc acattctcgg ccacgtcgag cgtcgcgatg
     2881 aggttgaaga actggtagac gaagccgacg tgcgcacgcc gatagagcgt gcgcgctggc
     2941 tcggcgagcg ccgtaatctc gacgccgtcc atctcgatcg tgccgctgtc ggcgcggtcg
     3001 atgccgctga tgagattgag gagggtcgac ttgcccgagc cgctgcgccc catgatggcc
     3061 acgcgctcgc cggggcgcac ctcagcctcc agcccatcga gcgcgcggtg cacgtgcgcg
     3121 ccctcgtgga agctacggga gagttgccga attctcagca cgcgggtggg ttcctggggc
     3181 tgcattgtag aattgaccgg atgatcaccg tccaccacct caacaactcc cgttcccagc
     3241 gcgtgctgtg gctgctcgaa gagcttggtt gcgagtacag cctcgtgaaa taccagcgcg
     3301 acccgcacac cctgctcgcg ccgccggagc tgcggcgcat ccatcctctg ggaaagtccc
     3361 cggtgatcac cgacggcgcg cacacgctgg ccgaatccgg cgcgatcctc gagtacctgg
     3421 tcgatcgcta cggcgacgga cggctgcggc cgccgccagg aagcgccgag cagctgcgct
     3481 acacgtattg gctgcactac gcggaaggct cggcgatgcc gccgctgctg ctgaagctgg
     3541 tgttcctgcg cctgccccgc ccggtgccgg cgctcatccg tcccatcgtg cgcgcggtcg
     3601 cgcagcgggc gcagcagagt ttcatcgacc cccagctcaa gacgcacatc gactactggg
     3661 aggcggagct cggaaaatcg acctggttcg ccggcgagga gctcacggcg gccgatgtgc
     3721 agatgagctt tccgctggag gccgccgccg cgcgcgccgg gatcgcgctc ggcccgaggg
     3781 tcacccagtt cctcgcacgc atccacgcgc ggcccgccta ccggcgggcg ctcgagagag
     3841 gcggcgagtt ccgacttgca cggtaagcgc ccggtccgga gcgccgtcga cgacggggcc
     3901 tcctcagtcg tcgcacggcc cgcattatta tggagaatcg cgacgcgacg cgccttatgc
     3961 ggctggcacg ctgggggcta atcggcgaca ctctcgcccc gccgcggtcc tcaagccgtg
     4021 ccgtgcacgg cccgcaggct tccagaagga aaactcatga cattgccagc gcgacaccgc
     4081 ggccggatta ctctcaccct cgggcttgcg gcggccgccg tattattggt cgtttcgctc
     4141 cctgcgacgg cgcagcagcc cgccactccc gcagccgcca ctcccgcagc cgccacgccg
     4201 gccaccgccc ctgcggcggc aaagaaggcg aaggctcccg ccacgagcgc cgccgccgag
     4261 agcccggcca gcgcgaggag ctacagcctt ggcctcatgt ggggcgacca gctgcgcacc
     4321 acaggcgtcg cccccgatgg catcaatgcg gcgcgcatcg cccagggagt gcacgatgcg
     4381 atcaccggca aggtcgcgct gacagacaag gaccacgaga acatccgggc gctcgccaac
     4441 tcggccggcg actccaacca tcaggtcgcg gccaagttcc tggccgagaa cggcaagaag
     4501 ccgggcgtgg tgacgacggc gagcgggctg cagtaccagg tgc
//
LOCUS       DATLKL010000289         2564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_194854, whole genome shotgun sequence.
ACCESSION   DATLKL010000289 DATLKL010000000
VERSION     DATLKL010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194854"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2108)
                     /locus_tag="VM713_03770"
     CDS             complement(<1..2108)
                     /locus_tag="VM713_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HVH83417.1"
                     /translation="MQTARDITGYRKYWAERFGTAPFLPMSRAEMDLLGWDSCDVIIV
                     TGDAYVDHPSFGMAIVGRVLEAQGFRVGIIAQPDWHDAAPLAALGRPNLFFGITGGNM
                     DSMVNRYTADRRVRSDDAYTPGGVAGRRPDRSVIVYAQRAREAFRDVPIVIGGIEASL
                     RRIAHFDYWSEKVRRSVLLDAKADMLVYGNGERQICEIAHRLAGGETIGEITDVRGTV
                     FVRRGTPADWIEIDSTHLDAPGPLNPPVDPYAMTDRAPGSAAPVASGGTPACAGAPSA
                     PATEHVVRFVRRVKNADRERSVIRMPGCEAVAADPVLYAHASRILHLESNPGNARALT
                     QRHGDVDVWLNPPPLPLTTAEMDWVYERPYARAPHPCYGKAQIPAYKMIRFSVAIQRG
                     CFGGCTFCSITEHEGRIIQSRSETSVLREIETVRDTVPGFTGVISDLGGPTANMYRLA
                     CRSREIESACRRPSCVYPGICPNLDTDHAPLIRLYRRARALPGIKKVLIASGVRYDLA
                     IESPEYVKELAQHHTGGYLKIAPEALAEGPLSKMMKPGVAAYDRFKELFDRYSKEAGK
                     EQYLIPYFIAAHPGTSDADMLELALWLKKNGFRADQVQAFLPGPMATATAMYHSGKNP
                     LRRITRSSEEVLIPKGLKVRRLHKAFLRYHDPENWPVLREALRRMGRADLIGHGRQQL
                     IPAYQPAGAGGAARRRKAGAA"
     gene            2304..2561
                     /locus_tag="VM713_03775"
     CDS             2304..2561
                     /locus_tag="VM713_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83418.1"
                     /translation="MKLITRTQTGVRLESHLLKVLKALATELDLSLGDLLEGIVLHAF
                     EGKAPFSASTLKKVKALKAVYGLDLTARDSHQLREAGQDAP"
BASE COUNT          398 a          856 c          901 g          409 t
ORIGIN      
        1 gccgcccccg ccttgcggcg ccgcgccgcg ccgcccgcgc ccgcgggctg gtaggcggga
       61 atcagctgct ggcggccgtg gccgatcagg tccgcacggc ccatgcgccg cagcgcctcg
      121 cgcagcacgg gccagttctc cgggtcgtgg tagcgcagga atgccttgtg gaggcggcgc
      181 accttgagac ccttcggtat caacacctct tcgctcgagc gcgtgatgcg cctcagcgga
      241 ttcttgcccg agtgatacat ggcggtcgcg gtggccatgg ggcccggcag gaacgcctgc
      301 acctggtcgg cgcgaaagcc gttcttcttc aaccacagcg cgagctccag catgtccgca
      361 tccgaggtgc ccggatgcgc ggcgatgaaa taaggaatca ggtactgctc cttgcccgcc
      421 tccttcgagt agcggtcgaa gagctccttg aagcggtcgt aggcggcgac cccgggcttc
      481 atcatcttcg agagcgggcc ctcggcgagc gcctcgggcg cgatcttgag gtagccgccg
      541 gtgtggtgct gggcgagctc cttcacgtac tcgggcgact cgatcgcgag gtcgtagcgg
      601 acccctgagg cgatcagcac cttcttgatt ccggggagcg cccgggcgcg acggtagagc
      661 cggatcagcg gtgcgtggtc ggtgtcgagg ttcgggcaga ttccggggta cacgcacgag
      721 gggcggcggc aggcgctctc gatctcgcgg ctccggcagg cgaggcgata catgttggcg
      781 gtcgggccgc cgaggtcgga gatcaccccg gtgaagcccg gtacggtatc gcgcaccgtc
      841 tcgatctcgc gcagcacgga agtctcggag cggctctgga tgatgcggcc ctcgtgctcg
      901 gtgatcgagc agaacgtgca gccgccgaag cagccgcgct ggatcgcgac cgagaagcgg
      961 atcatcttgt aggcggggat ctgcgccttg ccgtagcaag gatgcggggc gcgcgcgtac
     1021 ggccgctcgt acacccagtc catctcggcc gtggtgagcg gcagcggcgg gggattcagc
     1081 cacacatcga cgtcgccgtg acgctgcgtg agcgcccggg cgttaccggg gttggactcg
     1141 agatgcagga tccgcgaggc gtgcgcgtac agcaccggat ccgccgccac cgcctcgcag
     1201 cccggcatgc ggatgacgct gcgctcgcga tcggcattct tcacccggcg cacgaagcgc
     1261 acgacgtgtt cggtggcggg cgccgacggc gcgccggcgc aagctggcgt cccgccggaa
     1321 gccacgggcg cagctgaccc gggggcgcgg tcggtcatcg cgtaggggtc gaccggggga
     1381 ttgagaggcc cgggcgcatc gagatgcgtc gagtcgatct cgatccagtc cgcgggcgtg
     1441 ccacgccgca cgaacaccgt gccacgcaca tcggtgattt cgccgatggt ctcgccgccg
     1501 gcgaggcggt gcgcgatctc gcagatctgc cgctcgccgt tgccatatac cagcatgtcc
     1561 gccttggcat cgagcagtac cgagcggcgc actttctccg accagtagtc gaagtgcgca
     1621 atgcgccgga gcgaggcctc gatgccgccg attacgatcg gcacgtcgcg gaatgcctcg
     1681 cgggcgcgtt gtgcgtagac gatcaccgaa cggtcggggc gcctgcccgc cactcctccg
     1741 ggcgtgtagg cgtcatcgct ccgtacgcgg cggtcagcgg tatagcggtt gaccatggaa
     1801 tccatgttgc cgccggtgat gccgaagaac aggttcggcc ggccgagcgc cgcgagcgga
     1861 gcggcgtcgt gccagtcggg ctgcgcgata atgccgacgc ggaagccctg cgcctcgagc
     1921 acgcggccga cgatcgccat gccaaagctc gggtgatcga cgtaggcatc acccgtcacg
     1981 atgatgacgt cgcagctgtc ccagccgagg aggtccatct cggcgcgcga catgggcagg
     2041 aacggcgccg tgccgaagcg ctcggcccag tacttgcgat agccggtgat gtcgcgggcg
     2101 gtctgcatgc agccgagtag tttatcggga atcgcccgcc ttggccggga agctgtccgc
     2161 cgcgcagccg gcacaggcgc gagtcttacc gtgccttgcg ccgggacccc gcgacccgcc
     2221 gcaccgtgcc cgcccggcag ctggccgctc tggttcatgg tacgttggta ccatggtacc
     2281 aacaaccatg ataacatccg cccatgaagc tcattacccg cacccagacc ggcgtgcgac
     2341 tcgaatcaca tctgctcaag gttctgaagg cgctcgcgac cgagctcgac ctgtcgctcg
     2401 gcgacctgct cgaggggatc gtgctgcatg ccttcgaggg caaggcgccc ttctcggcgt
     2461 ccacgctgaa gaaggtcaag gcgctgaagg cggtctacgg gctcgacctc accgcgcgcg
     2521 actctcatca actgcgcgag gccggccagg atgcaccctg actg
//
LOCUS       DATLKL010000290         1877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_217428, whole genome shotgun sequence.
ACCESSION   DATLKL010000290 DATLKL010000000
VERSION     DATLKL010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217428"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="VM713_03780"
     CDS             complement(<1..1231)
                     /locus_tag="VM713_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83419.1"
                     /translation="MRRLSCLPLLLALATPVYGDSFGFATLAALIRTHDVGTVEELLA
                     ALPEAQRRNYALMFDSRSLQAASFENPRVILYGPDARFVLTFNGAPTQRGFRVVETLE
                     FDAVTREFRLRELEFPERPGGAAAVRVSEVNPERCAGCHGNPSRPLWDSFPLWPGAYG
                     ERYGASLSERERAGLARYLAQQQTHPRYRYLLDVDRYADSRTFRPGAGGQYAATVREP
                     PNAELSADLGELQFQAIAARLMRQPAFATYQFALLGVADGGCAPLSDFYPEGLWRAER
                     AAFARLGRDTARANAQQAQRKAERVAADVQAPPAPAASDTLLPLRFVAESALGIPTGS
                     WTLALESGTYDFTRPPAAALPLRASLLEALAPRDRALETLSYSATSADGDRYCSYLKR
                     RSRAALAGAEDTVNTMAA"
     gene            1469..>1877
                     /locus_tag="VM713_03785"
     CDS             1469..>1877
                     /locus_tag="VM713_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83420.1"
                     /translation="MGEIAPSDALPAAQPAARARGSTLTRSHDRLIALQFLAVVVPIA
                     FVLLAQMVADARRAAALAESRPLRTLANDARADYRTFTNGAADAVDTGSLGRQSAEAL
                     KNASDRLTELALRDGEPAVNKTAGQVADLARLLG"
BASE COUNT          293 a          633 c          676 g          275 t
ORIGIN      
        1 gtgccgccat ggtgttcacg gtgtcctcgg cgcctgccag ggccgcgcgg ctgcgccgct
       61 tgaggtaact gcagtagcga tcgccatcgg ctgaggtcgc ggagtagctg agcgtctcga
      121 gcgcgcgatc tcgcggggcg agtgcctcga gcagtgaggc gcgcagcggc aacgccgcgg
      181 caggtgggcg cgtgaagtcg tacgtgccgc tctcgagggc cagcgtccag ctgcccgtcg
      241 ggatgccgag cgccgactcg gccacgaagc gcagcggcag cagcgtgtcg ctcgcggcgg
      301 gcgcgggcgg cgcctgaacg tccgccgcaa cgcgttccgc cttgcgctgt gcctgctgcg
      361 cgttggctcg agccgtatcg cgcccgagtc gcgcgaaggc cgcgcgctcg gcacgccaca
      421 agccctccgg atagaagtcg gagagcggcg cgcagccgcc atcggcgacc ccgaggagcg
      481 cgaactgata ggtcgcgaag gccggctgac gcatgagacg cgcggcgatg gcctggaact
      541 gcagctcccc gagatcggcc gagagctcgg cattgggtgg ctcgcgcacg gtcgcggcgt
      601 actgaccgcc cgcacccggt cggaaggtac gcgagtcggc gtagcggtcg acatcgagca
      661 ggtagcggta gcgcgggtga gtctgctgct gcgcgaggta gcgtgcgagt cccgcgcgct
      721 cgcgctccga aagactggct ccgtagcgct ccccgtacgc ccccggccag agcggaaaac
      781 tatcccagag cgggcgcgat ggattcccgt ggcagcccgc gcagcgctcg gggttcacct
      841 ccgacacacg cacggcggcg gcgccccccg gccgctccgg gaactcgagc tcgcgcaggc
      901 ggaactcccg ggtcacggcg tcgaattcca gggtctcaac cacgcgaaag ccgcgctgcg
      961 tgggcgcgcc gttgaaagtc agaacgaagc gcgcgtccgg gccatagaga atgacgcgcg
     1021 gattctcgaa gctcgccgcc tggaggctgc ggctgtcgaa catcagagca tagttgcggc
     1081 gctgcgcttc agggagcgcc gccagcagct cttcgacggt tccgacatcg tgtgtgcgga
     1141 tcagcgccgc gagtgtcgca aatccgaagc tgtcgccgta gacgggggtg gcaagcgcga
     1201 ggaggagcgg caggcaggat aggcggcgca cggctcggat cattcccacg gccggggggt
     1261 ggcgaggatg cccccaggcc ttcccgaata gtgttcgggg gatcacggtg cgcggatgtt
     1321 acaccgcgta acttgacaaa gcgtgaccag catctcgtct ggaaccccct gaagatgtta
     1381 tcgatttccc tgtttagtgt ggtcaaccgg tcggcctcga ggggaccggg tctaacggag
     1441 cgcgcctcgg aattttttta tgctcgcagt gggtgaaatt gcaccctcgg acgcactgcc
     1501 tgcggcccaa ccggccgcac gggcgcgcgg gtctacgctc acacgctccc acgaccggct
     1561 gatcgccctg cagttcctcg cggtcgtggt gccgatcgct ttcgtgctgc tcgcacagat
     1621 ggtggccgat gcacgccgtg cggcggcgct ggccgaatcg cgcccgctgc gcacgctcgc
     1681 caacgacgcg cgggccgact accgcacctt caccaacggc gccgccgacg cggtcgatac
     1741 ggggtctctg ggccggcagt cggccgaggc actcaagaac gcctccgacc ggctcaccga
     1801 gctggccctg cgcgatggcg agccggcggt caacaagacg gccgggcagg tggccgacct
     1861 cgccaggctc ctcggca
//
LOCUS       DATLKL010000291         3368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_3260, whole genome shotgun sequence.
ACCESSION   DATLKL010000291 DATLKL010000000
VERSION     DATLKL010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3260"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VM713_03790"
     CDS             <1..758
                     /locus_tag="VM713_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVH83421.1"
                     /translation="YQAGELVVANVGWQDYGVSDGRDLVRINPTLERPSYALGVLGMP
                     GLTAYVGLLDIGGPKEGETVVVAAATGAVGSVVGQIAKLKGCRAVGVAGGAAKCDYAV
                     RVLGFDACLDHRAPELRERLAAACPQGIDIYFESVGGEVLYAAMPLLNVGARVPLCGL
                     IAWYNLEKLPAGVDRTPALLSAVLRRRIRIQGFIIFDHYNRFPAFVKDMSEWLRAGRV
                     HYREEVIEGLENAPRGLMGLLQGENFGKLVVRV"
     gene            770..1411
                     /locus_tag="VM713_03795"
     CDS             770..1411
                     /locus_tag="VM713_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HVH83422.1"
                     /translation="MSERITVSWVFDVISPFAYLGLHDLERLPAHIDLRFVPVLFAGM
                     LSHFGQVGPAEIPSKRRFTNRFVLWRARQLGLPMRFPPAHPFNPLAALRLVLAAGSDR
                     RAAAMVLEAVFGEGRDVTDPRVIAELAGRLGVADVERTLSEPAVKQRLRDNTDWAISL
                     GIFGVPTLVIGRELFWGHDAFGMALDYLNDRAGFVDPEMQALETLRVGVERRR"
     gene            1408..2934
                     /locus_tag="VM713_03800"
     CDS             1408..2934
                     /locus_tag="VM713_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83423.1"
                     /translation="MSPARLRALVVLALTAGPAAASGTDYWAYQYKNIDVTVAGSSDY
                     ARNVARNADRLGAALSRILNLKSAARLPTHIYVLADEQIVELLGSSGSSNYNSSGYDA
                     TVIASRGRGGDDAYWGVYFGYVGSLLAGDGGMRYPYWLRLGVPEVFATTQFDHDRIRT
                     GAIAPGYALTLASGTPIPLRTFLGMQEQDPQLQAGPPHEMYAAESWYLTREILVEGRH
                     RDEFSRYLGLLHQGRSERAAFAASFNVSYEDLDRMLREDQRTSGHVFVLASPTDERLE
                     AAPPRKLSPLEVKTCLALINLRAGRRAQALHLVDDALRDDPANEQALRVAAQAQLEEG
                     NYLASFAAVDQLSRGALSPDGMAESAAILTVLASAVSGGHAVLGVDAQKLLRYAQRYY
                     QGAIDANPDDLRSWAGLAGLYGAQRDRTAAQSLLPAASQALARHPSNVNLAYALTHMC
                     AQTQQWECAAEFASAWRENALTEPSRAEAAAFESRLSTYRRRLASAPAADSIPLPTQR
                     "
     gene            3066..>3368
                     /locus_tag="VM713_03805"
     CDS             3066..>3368
                     /locus_tag="VM713_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83424.1"
                     /translation="MNRDATFTRASRLTAPLCRWSLLACFLWPLYSSSAAAAPPPPPA
                     RIDLDAFEAMKKSVALPDGETLAYLSMGDPDGTPVVLIHGYTDSARDWVPLIPYLSP"
BASE COUNT          501 a         1138 c         1185 g          544 t
ORIGIN      
        1 cctatcaggc cggcgagctg gtggttgcca atgtcggctg gcaggactac ggggtgtccg
       61 acgggcgcga tctcgtcagg atcaatccca ccctcgagcg gccttcctac gcgctcggcg
      121 tgctcggcat gcccgggctc accgcctacg tgggactgct cgacatcggc gggccgaagg
      181 agggcgagac ggtcgtggtc gcggccgcca ccggagcggt gggatcggtg gtcggacaga
      241 tcgcgaagct caagggctgc cgcgccgtgg gcgtcgccgg gggcgcggct aaatgcgact
      301 acgcggtgcg cgtcctcggc ttcgacgctt gcctggacca tcgggcgccg gagctgcggg
      361 agcggctggc cgcggcctgt ccgcagggga tcgatatcta tttcgagagc gtcggcggcg
      421 aggtgctcta tgccgcgatg ccactcctca acgtcggggc ccgtgtgccg ctgtgcgggc
      481 tcatcgcctg gtacaacctc gagaagctcc ccgcgggcgt cgatcgcacg ccggcgctgc
      541 tctcggcggt gctgcgcagg cgcatcagga tccagggatt catcatcttc gaccattaca
      601 atcggttccc tgccttcgtg aaggacatga gcgagtggct gcgcgccggg cgcgtgcact
      661 accgggagga agtgatcgaa ggcctcgaga acgcgccccg tggactcatg gggctcctgc
      721 agggcgagaa cttcggcaag ctggtggtgc gcgtctgagg tgtccggaca tgagcgagcg
      781 catcacggtc tcctgggtgt tcgatgtcat atcccccttc gcctatctcg gcttgcacga
      841 tctcgagcgc ctgccggcgc acatcgacct gaggttcgtg ccggtgctgt tcgccgggat
      901 gctgtcgcac ttcgggcagg tcggacccgc cgagatccca tcgaagcggc ggttcaccaa
      961 ccgcttcgtc ctgtggcgcg cgcggcagct gggcctgccg atgcgctttc ctcccgcgca
     1021 tcccttcaac ccgctcgcgg cgctgcgcct ggtgctcgcg gccgggagcg accggcgcgc
     1081 cgccgcgatg gttctcgagg cggtgtttgg ggagggccgc gacgtgaccg acccccgggt
     1141 gatcgccgag ctcgcaggtc ggctgggggt cgccgatgtg gagcgcacgc tgagcgaacc
     1201 ggcggtgaag cagcggctgc gcgacaacac cgactgggcg atctcgctgg gtatcttcgg
     1261 cgtgccgacg ctggtgatcg ggcgcgagct cttctgggga cacgacgcct tcggcatggc
     1321 gctcgattac ctgaacgacc gggcagggtt tgtggacccc gagatgcagg cgctggaaac
     1381 gctgcgggtc ggggtggagc ggcggcgatg agtcccgcgc ggctgcgggc gctcgtagtg
     1441 ctggcgctga cggccgggcc ggcggccgcg tcgggcacgg actactgggc ctatcagtac
     1501 aagaacatcg atgtgaccgt cgccggcagc agcgactacg cgcggaatgt ggcgcgcaac
     1561 gccgaccggc tcggggcggc gctcagccgc atactgaacc tcaagagcgc tgcgcgcctc
     1621 ccgactcaca tctatgtgct cgcggatgag cagatcgtcg agctgttggg gtcgagcggc
     1681 agctcgaatt acaacagctc gggatacgac gccacggtca tcgcgagccg tgggcggggc
     1741 ggcgacgatg cctactgggg cgtctacttc ggctacgtgg ggagcctcct cgcgggcgat
     1801 ggcgggatgc gctaccccta ctggctccgg ctcggcgtcc cggaggtgtt cgcgacgact
     1861 cagttcgatc acgaccgcat caggaccggc gctatcgctc ccgggtacgc gctgacgctc
     1921 gccagcggga cgccgatccc gctgcggacc ttcctcggca tgcaggagca ggacccgcag
     1981 ttgcaagcgg gcccgccgca cgaaatgtac gcggccgaga gctggtatct cacccgtgag
     2041 atcctggtag agggccgtca tcgcgacgaa ttcagccgct acctcgggct gctgcatcag
     2101 ggcaggagcg agcgggcggc gttcgccgcg agcttcaatg tgagctacga ggacctcgac
     2161 aggatgctgc gcgaagacca gcgcacctcc ggccacgtgt ttgtgctggc gagccccacg
     2221 gacgagcgcc tggaggcggc cccgccgcgt aagctcagcc cgctcgaggt aaagacgtgc
     2281 ctcgcgttga tcaacctgcg cgccggccgg cgcgcgcagg cgctacatct cgtcgatgac
     2341 gcgctccgcg atgaccctgc caacgagcag gcgctgcggg ttgcggcgca ggcacagctc
     2401 gaggagggca actacctggc gtcgttcgcc gcggtggacc agctctccag aggtgccttg
     2461 tcgccggacg ggatggccga gagcgccgca atcctgacgg tgctggcctc cgccgtgagc
     2521 ggcggccacg ccgtgctcgg ggtggatgcg cagaagctgc tgcggtacgc gcagcgctac
     2581 taccagggcg cgatcgacgc caacccggac gatctgcgct cctgggcggg gctggcgggg
     2641 ctctacggcg cgcagcgcga ccgcaccgcg gcgcagtcac tgctgcccgc tgcctcgcag
     2701 gccctggcgc ggcatcccag caacgtgaac ctcgcttacg cgctcaccca catgtgcgcg
     2761 cagacccaac agtgggagtg cgcggcggag ttcgccagtg cctggcgtga gaacgcgctc
     2821 accgagccga gccgcgccga ggccgccgcc ttcgaatcgc ggctcagcac ctaccgacgg
     2881 cgcctcgcca gcgcacctgc ggcggattcc atcccactgc cgacgcagcg ctagctgata
     2941 tatcagaggc gccactgccg tctgcggcgc gcgccgcgca aaagctgttg cccggtcggt
     3001 acagccgcta tatattatcg agaagataat attaaccacc ggccgcaatt ccgacgcgtg
     3061 ttagcttgaa ccgcgacgcg accttcaccc gggcttcccg cctgacggcg ccgttatgcc
     3121 gctggtcgct gctcgcgtgc ttcctgtggc cgctctacag ttcatcggcg gccgcggctc
     3181 cgcccccgcc tcccgcccgg atcgatctcg acgccttcga ggcgatgaag aagagcgtcg
     3241 cgctgcccga cggggagacg ctggcgtacc tgagcatggg ggatcccgac ggtacgccgg
     3301 tcgtgctgat ccacggctac accgacagtg cccgcgactg ggtgccgctc attccatacc
     3361 tctcgccg
//
LOCUS       DATLKL010000292         2007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_71080, whole genome shotgun sequence.
ACCESSION   DATLKL010000292 DATLKL010000000
VERSION     DATLKL010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71080"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VM713_03810"
     CDS             <1..254
                     /locus_tag="VM713_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVH83425.1"
                     /translation="FGVTSNAVLPGWVRTAMAERSAQTEAERRGVSIEQVWRERAAIY
                     PQNRVLEPREVAEVIAFLCSDAAGGVNGEAITVALGGIW"
     gene            complement(264..1979)
                     /locus_tag="VM713_03815"
     CDS             complement(264..1979)
                     /locus_tag="VM713_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HVH83426.1"
                     /translation="MGSSILLDLVGGVALLLWGLHMVHSGIVRAFGSGLRRFLGRALS
                     NRFLAFLAGLLATTLLQSSTATGLMTASFVTDGSVNLVPALAIMLGANVGTTLIVQVL
                     SFNVAAVAPVLFLIGVIAFKRGGQTRTRDLGRVAIGVGLMVLSLHILLDTLAPAESAP
                     AVRTVLGAITAEPLLCLAVAAALTWVAHSSVTVVLLVMSLAYSQFIAPEAALALVLGA
                     NFGSAINPLLEGSRGDNPASRRLPVGNLLNRAVGCLLVLPFLQPIAAALARIEPNPAR
                     MAADFHTAFNVALALVFILPLDAVAALLTRLLPERPRAADPSTPLYLDETALATPSVA
                     LACAARETLHIGDIIETMLRQTMTALTADDRKLVEEICRMDDAVDRLNEAVKLYITRL
                     TRESLGERDGLRAMEIVSFSINLEHVGDIIDKNLMEVASKKIKRQIAFSAEGAAELAA
                     FHQEVYDNLKLAFGVFMTGDLGSARQLVTEKATLRATEFAAAENHLARLREGRAESIE
                     SSSLHLDILRDLKRIHSHICSVAYPVLERAGALQPSRLRAADSATAAATAGDAAAATR
                     RRRKH"
BASE COUNT          339 a          648 c          716 g          304 t
ORIGIN      
        1 ccttcggggt cacatccaac gcagtgctgc ccggctgggt gcgcacggcg atggcggagc
       61 gctcggcgca gacggaagcc gagcggcgcg gcgtgagcat cgagcaggtg tggcgcgagc
      121 gcgccgcgat ttacccgcag aaccgggtgc tcgagccgcg cgaggtcgcc gaggtgatcg
      181 cgttcctgtg cagcgacgcc gctgggggcg tgaacggcga agccatcacc gtggcgctcg
      241 gcggcatctg gtagccgtcc cggtcagtgc ttccgccgcc gccgcgttgc tgcggcggca
      301 tctcccgccg tcgccgcggc ggtcgcggaa tccgctgccc tcagccggct cggttgcagc
      361 gcgcccgcgc gctcgagcac cggataagcg accgagcaga tatgtgaatg gatgcgcttc
      421 aggtcgcgga ggatgtccag atgcagggag ctggattcga tactctcggc acggccttcc
      481 cgcagtcgcg ccagatggtt ctcagccgcc gcgaactcgg tggcgcgtag cgtggccttc
      541 tccgtcacca gctggcgcgc actcccgagg tcgccggtca tgaagacgcc gaaggccagc
      601 ttgagattgt cgtagacttc ctggtgaaag gcggccagtt cggcggcacc ttccgccgag
      661 aacgcgatct gacgtttgat cttcttggac gcgacttcca tcaggttctt atcgatgatg
      721 tcgccgacgt gctccagatt gatggagaac gagacgatct ccatcgcccg cagcccgtcg
      781 cgctcgccca ggctttcgcg cgtgagcctg gtgatgtaga gcttcaccgc ctcattgagg
      841 cggtcgacgg catcgtccat gcgacagatc tcctcaacca gcttgcggtc gtcggccgtg
      901 agcgcggtca tggtctggcg cagcatggtc tcgatgatgt cgccgatgtg gagcgtctcg
      961 cgggcggcgc aggcgagcgc caccgacggt gtcgcgagag cggtctcatc cagatagagc
     1021 ggcgtcgacg ggtcggcggc cctgggtcgc tcgggcaaca ggcgcgtcag cagggccgcc
     1081 acggcgtcca gcggcagaat gaacaccaag gccagggcga cgttgaaggc ggtgtgaaaa
     1141 tccgcggcca tgcgcgcggg attgggctcg atccgcgcca gcgccgcggc gatcggctgc
     1201 agaaacggca gtaccaggag gcagccgacc gcgcggttca ggagattccc cacaggcagg
     1261 cggcggctgg caggattgtc gccgcggctt ccttcgagca gaggattgat ggcgctgccg
     1321 aagttggcgc cgaggacgag ggcgagcgcc gcctcgggtg cgatgaactg cgagtaggcc
     1381 agggacatca cgagcagcac gaccgtcacg ctcgagtggg ccacccaggt gagcgccgcc
     1441 gccacagcca agcacagcaa aggctcggcc gtgatggcgc cgagcacggt gcgtaccgcg
     1501 ggggcgctct ccgccggcgc cagcgtgtcg agcaggatgt gcagcgacag caccatgagt
     1561 ccgacgccga tggccactcg cccgaggtcg cgcgtgcgcg tctggccgcc gcgcttgaag
     1621 gcgattacgc cgatcagaaa cagtaccggt gccaccgccg cgacgttgaa cgacagcacc
     1681 tgaacgatga gcgtggtgcc gacattggcg ccaagcatga tcgcgagcgc cggcaccagg
     1741 ttcactgagc cgtcggtgac gaaggacgcc gtcatgagac ccgtagccgt gctgctttgc
     1801 aggagcgtcg tggcgaggag accggcgaga aaggccagga agcggttgct cagtgcccga
     1861 ccgaggaagc gccggaggcc cgagccgaac gcgcgcacga tgccgctgtg gaccatgtgc
     1921 aggccccaca ggagcagcgc gaccccgccc accagatcga gaaggatgct gcttcccacg
     1981 ttcggtgccc gcgccgttca gtcgttt
//
LOCUS       DATLKL010000293         2197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_3309, whole genome shotgun sequence.
ACCESSION   DATLKL010000293 DATLKL010000000
VERSION     DATLKL010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3309"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1737)
                     /locus_tag="VM713_03820"
     CDS             complement(130..1737)
                     /locus_tag="VM713_03820"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HVH83427.1"
                     /translation="MSTATLFNYSYDPSTGRDPNAGPPPVKRIVIVGGGTAGWMTALI
                     LARSFIERGVEITLLESPVVGIIGVGEGSTPWLRGFFDSLGIEESEWMPACNATYKCG
                     ISFDRWSTKPGFERYFHAFASMLDNLTLRQFVHNADARVNGADVHAHPDRFFVAARLA
                     ARQRAPKAPESFPFDIWHGYHFDAVLLGQFLHRKAVERGVRYVSCHVTQANLAADGSI
                     ASVSTREGETIEADLFVDCSGFSGLLIEKALHTPYVSFSSNLFNDAAVAMPTPIDAAI
                     PSETISTALRHGWAWKIPLTNRYGNGYVYSSSFCTQDEAETELRRHLGLLESDVEARH
                     LKMRIGRVTRHWNRNCLAVGLAQGFIEPLEATALLFIQQTAATFVEFLEKGDLGEEAH
                     QRFNERVNAHFEGTRDYIVTHYKTNSREDTEYWRANAANMNLSEELKQLYGLWMSGKG
                     IAAPVGQQLLGKGYPVFSWYSLLAGMGIFPERLQLRPPNAREGIYKMAEIDDLIERSS
                     AHFPDHRAALGHIPPRRTAPSLQVYFF"
     gene            complement(1899..>2197)
                     /locus_tag="VM713_03825"
     CDS             complement(1899..>2197)
                     /locus_tag="VM713_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83428.1"
                     /translation="NTFNVLGYFETAQFSARLAYTYRSAFYSGLDRNTAFFQDAVGNL
                     AASLGYQVNDSFTITLDGLNLNNPTLKYYALNTDQPRAFYKNGSQYYLNFRFKL"
BASE COUNT          353 a          702 c          757 g          385 t
ORIGIN      
        1 tggcgcccat acgcgagtac cgcggcctga gcgagcaggc catccgcagc gaggtgttca
       61 cgtcctggca gccggcggtg ttgcgcggcc tcgtcggtca ctggcgcgcc gtggctgcgc
      121 ggtacggcat cagaagaagt agacctggag cgagggcgcg gtgcggcgcg ggggaatgtg
      181 gccgagcgcc gcgcgatgat ccgggaagtg agcggagctg cgctcgatca ggtcgtcgat
      241 ctcggccatc ttgtagatgc cttcgcgcgc gttcggcggc cgcaactgca ggcgctcggg
      301 aaagatgccc atgccggcca ggagcgaata ccacgagaac accgggtagc ccttgccgag
      361 cagctgctgg ccgaccgggg cggcgatccc cttcccggac atccacagcc cgtagagctg
      421 cttgagctcc tcagagaggt tcatgttggc ggcattcgcc cgccagtact cggtgtcctc
      481 gcggctgttg gtcttatagt gggtcacgat gtaatcgcgc gtcccttcga agtgcgcgtt
      541 gacccgctcg ttgaagcgct ggtgcgcctc ctcaccgagg tcgcccttct cgaggaactc
      601 gacgaaggtc gccgcggtct gctggatgaa gagcagcgcg gtcgcctcga gcggctcgat
      661 gaacccctgc gccagcccca cggccaggca gttcctgttc cagtgacgcg taacgcgccc
      721 gatcctcatc ttgaggtgcc gtgcctccac gtctgactcg agcagcccca ggtggcggcg
      781 cagctcggtc tcggcctcgt cctgggtgca gaagctgctc gagtacacgt agccgttgcc
      841 gtatcggttg gtgagcggaa tcttccacgc ccagccatgc cgcaaggcgg tcgagatcgt
      901 ctccgacggg atcgccgcgt cgatcggcgt cggcatcgcc accgccgcgt cgttgaagag
      961 attactggag aagctcacgt agggcgtgtg cagcgccttc tcgatcaaaa gtcccgagaa
     1021 cccgctgcag tcgacgaaca gatcagcctc gatggtctcg ccctcgcgcg tgctgactga
     1081 ggcgatcgag ccgtcggccg cgaggttcgc ctgggtcacg tggcagctca cgtagcgcac
     1141 cccgcgctcc accgccttcc tatgcaggaa ttgcccgagc agcaccgcat cgaagtgata
     1201 gccgtgccag atatcgaagg gaaagctctc gggcgccttc ggcgcgcgct gccgggccgc
     1261 gagccttgcg gcgacgaaga agcggtccgg gtgggcgtgg acgtcggcgc cgttgacgcg
     1321 cgcgtccgcg ttgtggacga actgccgcag tgtcaggttg tcgagcatcg aagcgaaggc
     1381 gtggaaatag cgctcgaagc ccggcttggt cgaccagcgg tcgaagctga tgccgcactt
     1441 gtaggtggcg ttgcaggccg gcatccattc cgactcttcg atcccgaggc tgtcgaagaa
     1501 gccgcgcagc cagggcgtcg agccctcgcc cacgccgatg atgccgacca cgggcgactc
     1561 gagcagcgtg atctcgacgc cgcgctcgat gaaggagcgt gccaggatga gtgccgtcat
     1621 ccacccggcg gtgccgccgc cgacgatgac gatgcgcttg acggggggtg ggcccgcgtt
     1681 cgggtcacgg cccgtgctcg ggtcgtagga atagttgaac agcgtggcgg tgctcatggc
     1741 ttccgtctgg actggtcgcg ggcgtgagat cccccgcggc acgttactcg caccacgccg
     1801 cacccgccaa cctccctggg tcgggtcccc gttgagcccc gccgcccgga aagggggcgg
     1861 cattgcgccg cccgccatcc gggtcattcc ggcagtcctt agagcttgaa gcggaagttc
     1921 aggtagtact gcgaaccgtt cttgtagaac gcgcgcggct ggtccgtatt cagcgcgtag
     1981 tacttgaggg tcgggttgtt gaggttcaag ccgtccagcg tgatcgtgaa cgagtcgttc
     2041 acctggtagc cgagcgaggc cgccaggttc ccgacggcgt cctggaagaa cgccgtgttg
     2101 cggtcgaggc cgctgtagaa cgccgagcga taggtgtagg ccagccgcgc gctgaactgc
     2161 gccgtctcga agtaccccag cacgttgaag gtgttct
//
LOCUS       DATLKL010000294         3343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_93613, whole genome shotgun sequence.
ACCESSION   DATLKL010000294 DATLKL010000000
VERSION     DATLKL010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93613"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..443
                     /locus_tag="VM713_03830"
     CDS             180..443
                     /locus_tag="VM713_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003213503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83429.1"
                     /translation="MRKNSSGVALATAAAMLFGTALTSTASAADAPKAEGAKIHCTGV
                     NSCKGQSACKSANNACKGQNSCKGKGFVEMTKADCDAAKAKAK"
     gene            complement(526..1146)
                     /locus_tag="VM713_03835"
     CDS             complement(526..1146)
                     /locus_tag="VM713_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016657123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HVH83430.1"
                     /translation="MTPALTALAALREGNARFVAELREAHPVSPRRRELPAGQEPLAI
                     ILGCSDARVPAEIVFDQGLGDLFVIRVAGNIVAPSQIGSDEFAAAAFGTRLVVVLGHS
                     NCGAIHATLDQLNRPSRDQSPNLRSIVNRIRPAVEGLIERGAGSSRDDLVHRAVRANI
                     RMSADHLRHGSEVLEQAIAQDGLLVVGAEYSLESGVVEFFDGVPEG"
     gene            complement(1198..>3343)
                     /gene="hrpB"
                     /locus_tag="VM713_03840"
     CDS             complement(1198..>3343)
                     /gene="hrpB"
                     /locus_tag="VM713_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HVH83431.1"
                     /translation="LDARAQLRPDLRILVMSATLDGAAVARLLGDAPVVSVSGRAYPV
                     ETRFAGRGPPPLPGAHAGGGTSPEQLVARLTLRALREASGDVLVFLPGAREIRRVQEL
                     LEASALEPGVRLLPLYGELTGEQQDAALAPAAPGARKVVLATNIAETSLTIPGVRVVV
                     DSGLARRALFDPATGMSALVTRRISRASAEQRQGRAGRLGPGVCYRAWSEGAHEGLAA
                     FTPPEIVEADLAPLALELASWGTRDARTLGWLDAPPAAMLAAAAALLERLGALAADGR
                     ITPHGRGMARLGVHPRLAHLLLRARELGCLPLGAQLAALLSERDLLRGPAAGADADIR
                     TRLELVRGEGARAAVDRAALERVRRAARDLERQLAERPGRQSQRRAQRAGRDPQPGVG
                     LLLALAYPDRIGRHRAGGGGSFTLANGRGATFVEAQPLARRELIVAVDLDDRERDARI
                     RLAAPLERDELMEQFAARIRCIDSVAWSTREQAVVARRTLELDALVLEEKPLRDAPAE
                     AVREAMLAGVRELGLAALPWDREARDLQARSEFLRRAPARAGAAPWPDLSDGALAGAL
                     ELWLAPWLDGVTRRQHLAHVPLEKALRARLSFEQQRALEERAPAYLTVPSGSRIRVDY
                     QGDDAPAVAVRLQEVFGLAATPRVDGGRVAVTMRLLSPAHRPVQVTRDLASFWRSAYL
                     EVRKDLRGRYPKHHWPEDPLAAQPTRGVRRPR"
BASE COUNT          481 a         1229 c         1220 g          413 t
ORIGIN      
        1 ggtttctctc gactactggc tgcagaggcg ttttggcgcg cgcggaaccc ctccggtcgc
       61 ggcggtgggt gcattcggcg gccggacgta acaaaatacg cgcccccccg gtctgctcac
      121 ttgaccgggg gttcgagccc gggaagtcaa tacgtattca ctaactgagg tcataccaaa
      181 tgcgtaagaa cagctccggc gtagccctcg cgacagcggc ggcgatgctc tttggcacgg
      241 ctctgaccag caccgcctcg gccgcggatg cccccaaggc agagggagcc aagattcatt
      301 gcacgggggt caactcctgc aagggtcagt cggcgtgtaa gagcgccaac aacgcctgca
      361 agggacagaa ctcctgcaag ggcaagggct tcgtcgagat gacgaaggcc gactgcgacg
      421 ccgccaaggc caaagccaag taacagaccc tgaagcctac agccccggtg tggcggtccg
      481 ccgtgcgcgc cgccgcaccg gggtttgtta ttttccgcgg ggcaatcagc cctccggcac
      541 gccgtcgaag aactcgacca ccccgctctc gagcgaatac tcggcgccca ccaccagcag
      601 cccgtcctgt gcgatcgcct gctccagcac ctccgagccg tgacgcaggt gatcggccga
      661 catgcggatg ttggcacgca cggcccgatg gaccaggtcg tcgcgactgc tgccggcgcc
      721 gcgctcgatc aggccctcga cggccgggcg gatgcggttg acgatcgagc gcaggttcgg
      781 cgactgatcc ctgctcgggc ggttgagctg gtcgagtgtc gcgtgaatcg cgccgcagtt
      841 ggaatggccc agcaccacga ccaggcgtgt gccgaaggcc gcggcggcga attcgtcgct
      901 gccgatctgg gagggggcca cgatgttgcc cgccacgcgg atcacgaaca ggtcgccgag
      961 cccctgatcg aagacgatct cggcgggcac ccgggcatcg gagcagccga gaatgatcgc
     1021 gagcggctcc tgcccggcgg gcagctcgcg gcggcgcgga cttaccggat gcgcctcgcg
     1081 cagctccgcg acgaagcggg cgttgccttc acggagcgcg gcgagcgcgg tcagtgcggg
     1141 tgtcatggcg ccaccgtacc gggagcagcg ctataaagcc aacccgcgcg gcgcgtttca
     1201 gcgcggccgg cgcacgccgc gggtcggctg ggcggcgagc ggatcctcgg gccagtgatg
     1261 cttcgggtag cggccgcgca gatccttgcg cacctcgagg taggcgctgc gccagaagct
     1321 cgccagatcg cgcgtcacct gcaccgggcg gtgcgcgggc gataagaggc gcatggtgac
     1381 cgctacccgc ccgccgtcga cgcgtggggt cgccgccagt ccaaacacct cctgcagccg
     1441 caccgcgacc gccggcgcat cgtccccctg gtagtcgacg cggatgcgcg agccgctcgg
     1501 caccgtgagg tacgcgggcg cgcgctcctc gagcgcgcgc tgctgctcga agctcaagcg
     1561 cgcccgcagc gccttctcga gcggcacgtg cgcgagatgc tggcgccgcg tcactccgtc
     1621 gagccacggc gcgagccaga gctcgagcgc gcccgcgagg gcgccgtccg agaggtcggg
     1681 ccagggtgct gctccggcac gcgcgggcgc ccggcgcagg aactcgctgc gcgcctgaag
     1741 atcgcgcgcc tcgcggtccc acgggagcgc cgcaagtccg agctcgcgca cccccgcgag
     1801 catcgcctcc cggaccgcct ccgcgggcgc gtcgcggagc ggcttctcct cgagcacgag
     1861 cgcgtcgagc tcgagcgtgc gccgggcgac gaccgcctgc tcgcgcgtgc tccaggcgac
     1921 cgagtcgatg cagcggatgc gcgcggcgaa ctgctccatg agctcgtcgc gctcgagcgg
     1981 ggcggcgagc cggatgcgcg cgtcgcgctc gcgatcgtcc aggtcgacgg cgacgatgag
     2041 ctcgcgccgc gcgagcggct gtgcctcgac gaaggtcgca ccgcggccgt tggcgagcgt
     2101 gaagctcccg ccccctccgg ctcggtgccg cccgatccgg tcaggatagg cgagcgcgag
     2161 cagcagtccg acgcccggct gcggatcgcg gccggcgcgc tgggcgcggc gctggctctg
     2221 ccgcccgggc cgctccgcga gctgccgctc gagatcccgc gccgcgcgcc gcacgcgctc
     2281 gagcgccgcc cggtcgacgg cggcgcgcgc tccctcgccc cgcaccagct cgaggcgcgt
     2341 gcggatgtcg gcgtcggcgc cggccgcggg tccgcgcaac aggtcgcgct cgctaagcag
     2401 cgccgcgagc tgagcgccaa gcggcaggca gccgagctcg cgcgcgcgca gcagcaggtg
     2461 cgcgagccgt ggatgcacgc ccagacgcgc catgccgcgc ccgtgcgggg tgatgcgtcc
     2521 gtcggctgcc agcgccccga ggcgctcgag cagcgctgcg gccgccgcga gcattgccgc
     2581 cggcggcgca tcgagccagc cgagcgtgcg cgcatcgcgc gtgccccagc tcgcgagctc
     2641 gagggcgagc ggcgcgagat ccgcttcaac gatctcgggc ggcgtgaacg ccgcgaggcc
     2701 ctcatgggca ccctcgctcc aggcccggta gcagacccct gggcccagcc gtccggcgcg
     2761 gccctgccgc tgctcggcgg aggcgcgcga gatgcgccgc gtcacgagag cgctcatccc
     2821 ggtcgcggga tcgaacagcg cccggcgcgc cagacccgag tcgaccacca cccgtacgcc
     2881 cgggatcgtg aggctggtct cggcgatgtt ggttgcgagc acgaccttgc gggcgcccgg
     2941 cgcggcggga gcgagcgccg cgtcctgctg ctcgccggtg agctcgccgt acagggggag
     3001 aaggcgcaca ccgggctcca gcgcgctcgc ttcgaggagc tcctgcacgc ggcggatctc
     3061 gcgcgctccg ggcaggaaca ccagcacgtc acccgatgcc tcgcgcagcg cgcgcagcgt
     3121 gaggcgcgcc acgagctgct cgggcgaagt gccgccgcca gcgtgcgcgc cggggagcgg
     3181 gggcgggccc cggcccgcga aacgcgtctc caccggatag gccctgcctg agacactcac
     3241 gacgggcgcg tccccaagga ggcgcgcgac ggcggcgccg tccagcgtcg ccgacatcac
     3301 caggatccgc aggtcgggcc gcagctgcgc gcgcgcatcg aga
//
LOCUS       DATLKL010000295         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_161202, whole genome shotgun sequence.
ACCESSION   DATLKL010000295 DATLKL010000000
VERSION     DATLKL010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161202"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VM713_03845"
     CDS             <1..591
                     /locus_tag="VM713_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HVH83432.1"
                     /translation="GLLGLPNAGKSTLIRAVSAARPKVADYPFTTLHPNLGVVAVGAH
                     RSFVMADIPGLIEGAAEGAGLGHRFLRHLQRTRLLLHLVDIAPLDPQADPVRDARAIV
                     AELRKFSRELAAKPRWLVLNKRDLLPQDEADQRAREIVRRLRYRGPRFLISGATGAGT
                     KELCEAVMRLLEEDARAARARASSPAGTGAAPRAAT"
     gene            complement(651..914)
                     /gene="rpsT"
                     /locus_tag="VM713_03850"
     CDS             complement(651..914)
                     /gene="rpsT"
                     /locus_tag="VM713_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HVH83433.1"
                     /translation="MANTKSAEKAARQAEKHRARNVALRSRMRTAVRKVTTAITAGDK
                     VAAQNTYKEAVPVIDTLVGKQIIHRNKAARHKSRLSARIRAMQ"
     gene            1093..2037
                     /locus_tag="VM713_03855"
     CDS             1093..2037
                     /locus_tag="VM713_03855"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HVH83434.1"
                     /translation="MQLIRGQVDLPRSHQGCALTIGTFDGLHLGHQALIARLLSHAQR
                     LGEPAVMLTFEPMPREFLSPGDPPARLTTFRERWRILAKAGIDYLWLLHFGEALRNLS
                     AEDFAQLLARRLRPRVVVVGHDFRFARKGEATAAALAAAGERLGFAVDIVPAVRLAGE
                     RVSSSGVRAALACGDFARAERWLGRRYSMRGRVIGGKRLGRDLGFPTANLALGRRRAP
                     VAGIFAVQVHGAANAALPGVASLGTRPTIGGGEALLEAHVFDFSDDLYGREIEVEFAA
                     KLRDEQRFATLEALTTQMQRDAADARRILGGARTGLNG"
     gene            2040..>2725
                     /locus_tag="VM713_03860"
     CDS             2040..>2725
                     /locus_tag="VM713_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVH83435.1"
                     /translation="MADYKHTINLPRTDFPMKADLAQREPAMARAWEERGTYAKLREL
                     ARGRPRFVLHDGPPYANGAIHIGHAVNKILKDVVVKSRSLDGFDSPYIPGWDCHGLPI
                     ELQVEKKHGRPGQKLDAQAFRAACRAYAAEQIDLQRSDFKRLGVLGDWDRPYMTMSPR
                     YEAQQLRAFGRIIGNGHLYKGVKPVHWCTECRSALAEAEVEYEERTSPAIDVAFRVSD
                     NADLARRVSLG"
BASE COUNT          402 a          909 c          977 g          437 t
ORIGIN      
        1 gggctgctcg ggctccccaa tgccggcaag tccacactca ttcgggcggt gtcagccgcc
       61 cggcccaagg tggcggatta tcctttcacg acgctgcacc cgaacctggg ggtggtggcg
      121 gtgggcgcgc accgcagctt cgtgatggcc gatatcccgg ggctcatcga gggggcggcg
      181 gagggggccg ggctcgggca ccggttcctg aggcatctgc agcgcacacg gctgctcctg
      241 cacctggtgg acatcgcgcc gctggacccg caagccgatc ccgtgcgcga tgcgcgcgcc
      301 atcgtggcgg agctcagaaa attcagccgt gagctggcgg cgaagccgcg ctggctggtg
      361 ctcaacaagc gcgacctgct gccgcaggac gaagcagacc agcgagcgcg cgagatcgtg
      421 cgccgactgc gctaccgcgg accccgcttc ctcatctcgg gagcgacggg ggccggaacg
      481 aaagagctgt gcgaggcggt tatgaggctt ttggaggagg acgctcgcgc ggcacgcgcg
      541 cgcgcatcat ctccagcagg aactggcgcc gcgccgcggg ccgcaacttg agcggcgtcg
      601 gcacgacggg gcttggaatc cgggtcttgt cattcacgtt catcgcagcc tcattgcatc
      661 gcgcgaattc gagccgaaag ccggcttttg tgccgggcgg ccttgttccg gtggatgatc
      721 tgcttgccaa cgagcgtatc gatgaccggg acggcttcct tgtaggtatt ctgagcggcc
      781 accttgtcac ccgcggtgat ggccgtggtc accttgcgaa cggcggtgcg catgcgggag
      841 cgcagggcaa cgttacgggc gcggtgcttc tcggcctggc gggcagcctt ttcggcggac
      901 ttggtattgg ccactcaatc tccgttatcg atagtccccc ggaggcgggg gccggtagtc
      961 tggcgagtgg gcatggcctt gtcaacgcaa accgccaggc tcggcgtgag cacttcacat
     1021 aaactaggcc gtgacctgcc gcggcgggcc agacttgact tagtccttcc gactcagtga
     1081 cttaaggagt tcatgcagct cattcgcgga caggtggacc tgccgcgcag ccaccaggga
     1141 tgcgcgctga ccattggcac cttcgacggc ctgcacttgg gtcaccaggc gctcatcgcg
     1201 cggctgttga gccacgcgca acggctcggg gagccggctg tcatgctgac cttcgaaccc
     1261 atgccgcgcg agttcctctc gcccggggat cctccggcga ggctcacgac gttccgcgag
     1321 cgctggcgga tcctcgccaa ggcaggcatc gactacctct ggctgctgca cttcggggag
     1381 gctttgcgta acctctccgc cgaggacttc gcgcagctcc tggcgcgccg cttgcgcccg
     1441 cgcgtggtcg tcgtcgggca tgatttccgc ttcgcgcgca agggcgaggc gactgcggcg
     1501 gcgctggcgg cggccggcga gcggctcggt ttcgccgtgg acatcgtgcc cgcggtgagg
     1561 ttggccgggg agcgcgtctc gagcagcggc gtgcgcgcgg cgctcgcgtg cggcgatttt
     1621 gcgcgcgcgg agcgctggct cggccggcgg tactcgatgc gcggccgcgt gatcgggggc
     1681 aagcgcctcg gaagggatct cgggtttcct accgcgaatc tggcgctcgg gcggcggcgg
     1741 gccccggtcg cgggcatctt tgcggtgcag gtgcacggcg cggcgaatgc cgcgctcccc
     1801 ggggtcgcca gcctcgggac gcgtccgacg atcggcggtg gcgaggcgct gctcgaggcg
     1861 cacgtgttcg atttcagcga cgatctctac gggcgcgaga tcgaggtgga gttcgccgcg
     1921 aagctgcgcg acgagcagcg cttcgcgacg ctcgaggcgc tcacgacgca gatgcagcgc
     1981 gatgccgcgg acgcgcgccg catcctcggc ggcgcgcgca cgggcctgaa cggatgacca
     2041 tggcggatta caagcacacc atcaacctgc cgcggacgga tttccccatg aaggcggacc
     2101 tcgcccagcg cgagcccgcc atggcgcgcg cctgggagga gcgcggcact tacgcgaagc
     2161 tgcgcgagct cgcgcgcggc cgcccgcgct tcgtgctgca cgacggcccg ccgtacgcga
     2221 acggcgcgat ccacatcggc catgccgtca acaagatcct caaggacgtc gtcgtcaagt
     2281 cgcggtccct ggacggcttc gactcgccct acattcccgg ctgggactgc cacgggctgc
     2341 cgatcgagct gcaggtggag aagaagcacg gccggccagg acagaagctc gatgcccagg
     2401 ccttccgcgc cgcctgccgc gcctacgccg ccgagcagat cgacctgcag cgcagcgact
     2461 tcaagcggct cggcgttctc ggcgactggg accggcccta catgaccatg tcgccgcgct
     2521 acgaggcgca gcagctgcgg gccttcggcc gcatcatcgg gaacggccac ctctacaagg
     2581 gggtgaagcc ggtgcactgg tgcaccgagt gccgctcggc gctcgccgag gcggaagtcg
     2641 agtacgagga gaggacttct ccggccatcg acgtggcctt ccgggtgagc gacaacgccg
     2701 atctggcgcg gcgggtctcc ctcgg
//
LOCUS       DATLKL010000296         1868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_184072, whole genome shotgun sequence.
ACCESSION   DATLKL010000296 DATLKL010000000
VERSION     DATLKL010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184072"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1702
                     /locus_tag="VM713_03865"
     CDS             233..1702
                     /locus_tag="VM713_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH83436.1"
                     /translation="MKFKRTPPIQAWAAQCAIGGAGLALITFVCFQLGLSNATAGFAY
                     MLLIAPLSLLGSFGASVILSIAAAACLNYFFTPPLFSFRVDAPQDALAIAAFTTTSLI
                     VTALTTRVRKSAEAARASQQALIDTIPAFVWTALPDGSRDFHNRRWVDFMGVSAAEAA
                     GEGWIGRLDPEDRAKVVEKWRSAVATGEPFEVEARFRSARGEYRTLLVRAAPLRDETG
                     TIVKWYGVTTDIEDRRRAAEALRASEEQLELLRTELARVSRVTTLGELTAAIAHEMKQ
                     PLAATITNAQTALRWLNAQPAGLAEAGQALGRIVKDGNRAGEVIDRIRALIKKAPPRE
                     EHLEINGAIREVIELARGEAVKNGVSVKTELAEGLPLIRGDRIQLQQVILNLILNAVE
                     ATSGIDGGARELQISSGRAGSDGVLVAVRDSGPGLDPKSADRLFESFYTTKPGGLGLG
                     LSICRSIIEAHGGRLWASANVPRGAIFQFTVPAYSDTAS"
BASE COUNT          329 a          578 c          642 g          319 t
ORIGIN      
        1 cgtggccatg atcatgaagg cggtcggcct caacccgcgg gggcggctca gtcagtggct
       61 gtcccggctc gctacctggt tcatgcagct ccgcgccgac cgcttggccc gcgccggcgc
      121 gtgagccgcg acttcaaacg ataagaactc gaagacacga ggtctgcgcg gtgccgggtg
      181 cctagtcctg cggctgccgt gagcgtcaat tcgaggcgcg cttcgaacag ggatgaagtt
      241 caagcgtaca ccccccatac aggcttgggc cgcgcagtgc gcaataggcg gtgcggggct
      301 tgcattgatc actttcgttt gctttcagct tggccttagt aatgcgacgg cgggatttgc
      361 gtatatgctc ctcatcgcgc cgctttcctt gctgggaagc tttggcgcat cggtcatcct
      421 gtcgatcgcc gccgcggcct gcctgaacta tttcttcacg ccgccgttgt tcagtttccg
      481 cgtggatgct ccgcaggatg ctctcgcgat agccgccttc acgacgacgt cgctcattgt
      541 gactgcgctc acgacgaggg tgcgcaagtc ggcggaggcc gctcgggcgt cacagcaagc
      601 gctgattgac acgattccgg cgttcgtctg gaccgcgctt cccgatggtt cgcgcgactt
      661 ccataaccgg cgctgggtgg atttcatggg ggtttcggcg gcggaggcgg ccggcgaggg
      721 ctggattggc cggctcgatc ccgaagaccg cgcaaaggtc gtcgagaaat ggcgctcggc
      781 ggtcgcaacg ggcgagccgt tcgaagtcga agcccgcttt cggagcgcca ggggcgaata
      841 tcgcacgctg ctggtgcgcg ccgcgccgtt gcgcgacgag acggggacca tcgtgaagtg
      901 gtatggcgtg accacggaca ttgaggatcg taggcgcgct gccgaagcgc tgcgcgccag
      961 cgaagagcag ctggagcttc tacgcacgga gcttgcgcgg gtctctcggg tgacgaccct
     1021 gggagagctg acggcggcca tcgcgcatga aatgaagcag cccctggccg ccacgatcac
     1081 gaatgcccag acggcgctgc gctggctgaa cgcccagccg gcgggtctcg cagaggccgg
     1141 gcaggcgctc ggtcgcatcg tcaaggacgg caatcgagcg ggcgaggtca tcgaccggat
     1201 ccgcgctctc atcaagaagg cgcccccacg agaggagcac ttggagatca atggagcgat
     1261 ccgcgaggtg atcgagctcg cccgcggcga agcggtgaag aacggcgtgt cggtcaagac
     1321 ggaactcgcg gagggcctgc cgctcattcg cggagaccga atccagctgc aacaggtgat
     1381 cctaaacttg attctcaacg cggtcgaagc cacgagcggc atcgacggcg gggcgcgaga
     1441 gttgcagatc agcagcggaa gagcggggtc ggacggcgtg ctcgtcgcgg tgcgggattc
     1501 cggcccgggc ctggacccga agagcgcgga ccgcctcttc gagtccttct acacgaccaa
     1561 gccgggcggt ctggggctgg ggctgtcgat ctgccgctcg atcatcgaag cgcacggagg
     1621 aaggttgtgg gcgagcgcga acgtcccgcg cggcgccata tttcaattca ccgtgccggc
     1681 ctattcggac accgcatcgt gaccgagtag tttggcacgc gagttcgccg attggttcat
     1741 ccggctccgc gcccatcgcc cggcgggcgc cgaggccata accccaacta cgtacaatca
     1801 aatagccaca acggcacgac gacacaacga atcaagcttg cgcggcgcgc aagagcgacg
     1861 cggtgtcg
//
LOCUS       DATLKL010000297         1612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_127509, whole genome shotgun sequence.
ACCESSION   DATLKL010000297 DATLKL010000000
VERSION     DATLKL010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127509"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VM713_03870"
     CDS             complement(<1..665)
                     /locus_tag="VM713_03870"
                     /inference="COORDINATES: protein motif:HMM:NF028265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83437.1"
                     /translation="MFDWTHSGAAILAAFLASLVEFIEALTIVLAVGAVRGWRPALLG
                     SFLGTVLLVILVAALGPLLGRFPLSWLQSAVGVLLLLFGMRWLRKSVLRAAGIVPLHD
                     ELASYAKEAERLRGEGGTRALWDTVGVAATFKAVMLEGMEVVFIVIAIGAAGRRLIPA
                     SLGAAAALFIVIILGAALHRPVARVPENSLKFSVGVLLSAFGVFWIGEGSGLAWPGGD
                     LAIL"
     gene            1113..>1612
                     /locus_tag="VM713_03875"
     CDS             1113..>1612
                     /locus_tag="VM713_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HVH83438.1"
                     /translation="MRTISIWDLPTRIFHWLLVACVASAYLTSSARPHGLQFTVHVAS
                     GYAVALLLVFRLLWGFVGGEHARFSAFVRGWKAVKAHLQGLLRLAPARALGHNAIGGW
                     MILLILATLLLIVVTGLLAQGIPGGGGPLSAVLPNALAKPVGAVHELLGSFIIYLAAF
                     HVAGALG"
BASE COUNT          271 a          563 c          511 g          267 t
ORIGIN      
        1 aggatggcga gatcgccgcc cggccacgcg agtccgctcc cttctccgat ccagaatacg
       61 ccgaaggccg agagcagcac gccaaccgag aatttgaggc tgttctccgg cacccgcgcg
      121 acgggcctgt gcagcgccgc cccgaggatg atgacgatga acagtgctgc ggcggcaccg
      181 agcgaagccg ggatcagccg ccgccccgca gcgccgatcg cgatcacgat gaagacgacc
      241 tccatacctt ccagcatcac cgccttgaat gtcgcagcca ctccgaccgt gtcccacagc
      301 gcacgggtgc caccctcgcc acggaggcgc tcggcctcct tggcgtagct cgccaactcg
      361 tcatgcagcg ggacgatgcc ggcggcgcgc agaacggact tgcgcagcca ccgcatgccg
      421 aacaaaagca ggaggacgcc gaccgccgac tgcagccagc tgagcgggaa gcggccgagc
      481 agcgggccga gcgccgcgac gaggatgacg agcaggaccg tgcccaaaaa actcccgagg
      541 agggcgggcc gccatccgcg caccgcgccc acggcaagca cgatcgtcag ggcctcgatg
      601 aactcgacga gcgaggccag gaatgccgcg aggatggcgg cgcccgaatg ggtccaatcg
      661 aacattgttc cgcgtccttc cgaaatgcgc gccttgtact gctggccaca ggcccgagac
      721 ctgcgttcgc tacgaaccgc cgcccagggc tgattctcgc ctcgcttcct gctcgatatg
      781 aaatgttgct gacgattgtc aaagcctcgc tccgcgccgg tctccgcctt cccgagcagg
      841 atgcgataaa gttggatcag tcattgtcgt cactcccgcg aaagcgggag cccatcgact
      901 ggtggacccc cgcttgcgcg ggggtgacga caggggagcg tctcaactac gttgcatttc
      961 gctctagcat agctccattg cgaactcttt ggggtcgtgc cgattcggcc ctaccccgcc
     1021 cgtcccgttc agcgcaggtt catttggtcc caccatcatg caccggctcg gctgagttcg
     1081 ggcgtaaggg gatatgaagg gacggaggcg cgatgcgcac gatctcgata tgggacctgc
     1141 cgacgcggat cttccactgg ctgctcgtgg cgtgcgtggc gagcgcgtat ctcacgagtt
     1201 cggcacgtcc gcatgggctc caattcacgg tccacgtggc gagcggctac gccgtcgcgc
     1261 tcttgctcgt cttccgcctc ctctggggct tcgtgggcgg cgagcacgcg cgcttctctg
     1321 ccttcgtgcg cggctggaaa gcggtcaaag cccatctcca aggtctcctg cgcttggcac
     1381 cggcgcgcgc cctcggacac aacgcgatcg gcggctggat gatcctgttg atcctcgcga
     1441 cgctcctgct catcgtcgtg accgggctgc tggcgcaagg catacccggc ggcggcgggc
     1501 cgctctccgc cgttctgccc aatgcgctcg ccaagccggt cggcgccgtc cacgagctgc
     1561 tcggcagctt catcatctac ctcgccgcgt tccacgtggc gggcgcgctg gg
//
LOCUS       DATLKL010000298         3171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_59765, whole genome shotgun sequence.
ACCESSION   DATLKL010000298 DATLKL010000000
VERSION     DATLKL010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59765"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..224)
                     /locus_tag="VM713_03880"
     CDS             complement(69..224)
                     /locus_tag="VM713_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83439.1"
                     /translation="MSRNAAGFDRRADAPHGSWERSAPWVAPLLTAAVLALATFVMFA
                     SCGAALI"
     gene            complement(221..1081)
                     /locus_tag="VM713_03885"
     CDS             complement(221..1081)
                     /locus_tag="VM713_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVH83440.1"
                     /translation="MTAVIEARGLTKSYGATRALDGVSFSVEPGRIVGLIGPNGAGKT
                     TALKAILGLTPFGGELRVLGLDPRSQRDELMRDVCFIADVAVLPRWLRVAQAVDFVEH
                     VHPRFDRSIAENFLAKTDIKRGSRVGQLSKGMVTQLHLALILAIDARLLVLDEPTLGL
                     DLLYRRQFYDTLLNDYFDKERTILLTTHQVEEVEQLFTDVIFINHGHFALNSSVEELG
                     ARYQQLTVSSDNVARARELRPFYEREIFGRVAMYFEGRSSAELGAFGELRTPSIADLF
                     VAKMQGGAST"
     gene            complement(1065..1511)
                     /locus_tag="VM713_03890"
     CDS             complement(1065..1511)
                     /locus_tag="VM713_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVH83441.1"
                     /translation="MSTETLTTATMDPQWDNSLPIYRQLRDRVVAMILEGVLKEGDPL
                     PSVRNVAADFRLNPLTVLKGYQQLVDEQLVEKRRGRGMFVSLGARKALMKDERQRFLE
                     DEWPKVYATIQRLGLSGEELLRAGAAAPAAPFPAAGAKGEQDDGGN"
     gene            complement(1538..1732)
                     /locus_tag="VM713_03895"
     CDS             complement(1538..1732)
                     /locus_tag="VM713_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83442.1"
                     /translation="MNALATLSRNIACATAAALICVLSSMAFVESTAVVPGTQPEFIS
                     VAHVSAPSWFGKPEPAVLVD"
     gene            2008..2988
                     /locus_tag="VM713_03900"
     CDS             2008..2988
                     /locus_tag="VM713_03900"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HVH83443.1"
                     /translation="MKPAVNVAITGAAGQIGYALAFRVASGALLGPDTRVRLHLLEIP
                     AALPALQGVLMELNDCAFPTLESASASDDANVAFRDCQVAMLVGARPRGPGMERKDLL
                     LANAQIFSAQGKALNQHADRSVRVLVVGNPANTNALITMANAPDLKPRNFTAMTRLDH
                     NRAVAQLAEKTGTHVSDIRRMTIWGNHSATQYPDLTHALVRGKSAPALVGREWIEQTF
                     IPTVQQRGAAVIKARGASSAASAASAAIDHVHTWVHGTATDDWVSMGVASDGSYGIEA
                     GVIYSYPVTIANGEYRIVPNLGVDEFSRKRMDATNAELREERDGVQHLLG"
BASE COUNT          528 a         1053 c         1080 g          510 t
ORIGIN      
        1 catagtgtcg agcgggccgg cgcggcgcgt gagcgcgctc gcgggcgcga tggttgcggc
       61 gttggcgttc atatgagtgc ggccccgcag ctggcgaaca tgacgaaggt ggcgagcgcg
      121 agcacggcgg cggtgaggag cggcgccacc cagggcgccg agcgctccca ggacccgtgc
      181 ggcgcgtcgg cgcggcggtc gaagcctgcg gcgttgcggc tcatgtggag gcgcctccct
      241 gcatcttggc cacgaacagg tcggcgatcg agggcgtgcg cagctccccg aaggcgccga
      301 gctcggcgga cgagcggcct tcgaagtaca tcgccacccg cccgaatatc tcgcgctcgt
      361 agaacgggcg cagctcgcgg gcccgggcga cattgtcgga gctcacggtg agctgctggt
      421 agcgggcgcc gagctcctcc accgaggagt tgagcgcgaa gtgcccgtgg ttgatgaaga
      481 tcacgtccgt gaagagctgc tcgacctcct ccacctggtg cgtcgtgagg aggatggtgc
      541 gctccttgtc gaaatagtcg ttcagcagcg tgtcgtaaaa ctggcggcga tacagcaggt
      601 cgagcccgag cgtcggctca tcgagcacta ggagacgggc gtcgatcgcc aggatcagcg
      661 ccaggtgcag ctgcgtcacc atgcccttgg agagctggcc gacgcggctg ccgcgcttga
      721 tgtcggtctt ggcgaggaaa ttctcggcga tgctccggtc gaagcgcggg tgtacgtgct
      781 cgacgaagtc gaccgcctgg gcgacgcgca gccagcgcgg cagcaccgcc acgtccgcta
      841 tgaaacatac gtcacgcatg agctcatcgc gctgcgagcg cgggtcgagc ccgagcacgc
      901 gcagctcgcc gccgaagggg gtgaggccga ggatcgcctt gagcgcggtg gtcttgcccg
      961 cgccgttcgg cccgatgagg ccgacgatgc ggcctggctc gacactgaag ctcacgccat
     1021 ccagcgcgcg cgtcgcgccg taggacttgg tgaggccgcg cgcttcaatt accgccgtca
     1081 tcttgctccc ccttggcacc cgcggccggg aaaggcgccg cgggtgcggc ggctccggcg
     1141 cgcagcagct cctcgcccga gaggcccaag cgctggatgg tcgcgtagac cttcggccac
     1201 tcgtcctcga gaaagcgctg ccgctcgtcc ttcatgagcg ccttgcgagc ccccaggctc
     1261 acgaacatgc cgcggccccg gcgtttctcc accagctgct cgtccacgag ctgctggtat
     1321 cccttgagca ccgtcagcgg attcaaacgg aagtccgcgg cgacgttgcg caccgagggc
     1381 agcgggtctc cctccttcag gacgccctcg aggatcatgg caaccacgcg gtccctcagt
     1441 tgccggtaga tcggcaggct gttgtcccat tgcggatcca tggtggccgt cgtgagcgtc
     1501 tcggtggaca aggaggctgt tgcgggaggt tagcgaacta atctaccaaa acggccggct
     1561 cgggcttgcc gaaccaggac ggcgcggaca cgtgagcgac gctgatgaac tcgggctggg
     1621 tgcccggaac aactgcggtc gactcgacga aggccatgct ggacagcacg cagatcaggg
     1681 cggcggcagt ggcgcaggcg atattgcgag acagtgtggc gagtgcgttc atggtcatct
     1741 cctctctggg tcttcctctt ctcggctccc tgcaccatgc aggtcgcctt ccttagtgat
     1801 gtactctagt acaacactac ttgagtgtca agctatttca gtagatgacc tccggggccc
     1861 gaaaatgggg gtctcacggc tcccggggcg tagtctccgc aggggacagg gctgggtgtc
     1921 ccggagaagc cgtttttgcc ggctcgccga gtcgcacacg ctagaatttc aggtcctttt
     1981 tttccctcgc cacgaagaaa atcccacatg aaacccgctg tgaatgtcgc gatcaccggc
     2041 gccgccggcc agatcggcta tgcgctcgcc tttcgcgtcg cctcgggagc tctgctaggg
     2101 cccgatacgc gcgtccgact acatctgctc gagatcccgg cggcgctgcc ggcactgcag
     2161 ggcgtgctga tggagctcaa cgactgcgcc ttcccgacac tggagagcgc gagcgcgagc
     2221 gacgatgcga acgtggcatt ccgcgattgt caggtggcga tgctggtggg cgcgcggccg
     2281 cgcgggccag gcatggagcg caaggacctg ctgctcgcca acgcgcagat cttctcggcc
     2341 cagggcaagg cgctcaacca gcacgccgac cgcagcgtgc gcgtgctggt ggtcggtaac
     2401 cccgccaaca ccaacgcgct gatcaccatg gcgaacgcgc ccgatctcaa gccgcgcaac
     2461 ttcacggcca tgacgcgcct cgatcacaac cgcgcggtgg cgcagctggc ggagaagacc
     2521 ggcacgcacg tgtcggacat ccgccgcatg acgatctggg gcaaccattc cgccacccag
     2581 taccccgacc tcacccacgc gctggtacgc ggcaagagcg cgcccgcgct ggtcggccgc
     2641 gagtggatcg agcagacctt cattccgacc gtgcagcagc gcggtgccgc cgtcatcaag
     2701 gcgcggggcg cctcctcggc cgcttcggcc gcctcggccg cgatcgatca cgtgcacacc
     2761 tgggtgcacg gcaccgccac ggacgactgg gtcagcatgg gcgtggcgtc cgacggcagc
     2821 tacggcatcg aggccggcgt catctactcg tatccggtca cgatcgcgaa cggcgagtac
     2881 cgcatcgttc cgaacctggg cgtcgacgag ttcagccgca agcgcatgga cgccacgaat
     2941 gcagagctcc gcgaggagcg cgacggagtg cagcacctgc tcggctgaaa tatggacagg
     3001 acgaccgaaa ccgccatgtt gactagcggg caaaggccgc gccttttgcc cgcgcgcgtg
     3061 ggcgcgcctt ggcctgctgg ctggcccgcc ggcacagccg gccaatagga gaacactgcc
     3121 atgttaggtg cgatcgtcac gctgctcgcg gtgctggccg ccgcctacgc g
//
LOCUS       DATLKL010000299         4338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_37229, whole genome shotgun sequence.
ACCESSION   DATLKL010000299 DATLKL010000000
VERSION     DATLKL010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37229"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /gene="recG"
                     /locus_tag="VM713_03905"
     CDS             <1..1777
                     /gene="recG"
                     /locus_tag="VM713_03905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HVH83444.1"
                     /translation="QQQGLARGIRLRAFGEARRGPLGLEMVHPEYRRLGGEAAPLEET
                     LTPIYPLTEGVPQGRLRALIGQALDELERSGLADLLPARAGLPSGLPTLREALAYLHR
                     PPREAQLAVLAAGRHPAQRRLAFEELLAHTLALRSRKRALKSDPAWELDDREGLARRL
                     IGALPFALTRAQARALGEVEADLATGVPMVRLLQGDVGCGKTVIAAAAAARAAGTGKQ
                     AALMAPTELLAEQHWRNFQDWFRPLGIAVALLSGGQAARTRRSALEGLASGEIRVVVG
                     THALFQEGIEFAALALVVVNEQHRFGVQQRLRLSEKGRGGGRLPHQLIMTATPIPRTL
                     AMTAYADLDISVIDELPPGRTPVKTVVVPEERRAEVVARIVGACGAGRQVYWVCPLID
                     ESDELRAQAAEETAAALAEALPGVRVGLVHGRMPPQPKDQAMLAFKSGKIQLLVATTV
                     IEVGVDVPNATLMVIENAERMGLAQLHQLRGRVGRGAEVSTCVLLYRPPLSEPAHERL
                     KAIRATNDGFEIARRDLELRGPGELLGTRQTGLAQLRVADLMRDADLLPAVQAAAQLL
                     ESHPESSAALTARWIGSGERYGRVG"
     gene            1792..2760
                     /gene="ubiA"
                     /locus_tag="VM713_03910"
     CDS             1792..2760
                     /gene="ubiA"
                     /locus_tag="VM713_03910"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HVH83445.1"
                     /translation="MAAAAPELQATTPAPGLAETPTPAPRFREAPLSWRLTRRLTQYG
                     RLMRLDRPVGTWLLLWPALWALWVAGAARPKPNVLIIFVLGVVVMRAAGCVINDFTDR
                     DLDPYVRRTRDRPLAARLVTPVEALFLFAVLIAVALYLVTRLDEFTVKLAAIGALLTI
                     SYPFVKRVFPMPQLYLGISFGGWAVPMAFAAQLGTLPRVCWVLYIAAVIWAAMYDTIY
                     AMIDREDDIKIGVKSSAILFGDMDRLLIGVMQLMMLYALYLVGEDMHYSRWYRGGVIA
                     AAELFLWQQWLIRRRDPAGCLRAFLNNQYVGLVIFAGLLLQYVYKS"
     gene            2771..3133
                     /locus_tag="VM713_03915"
     CDS             2771..3133
                     /locus_tag="VM713_03915"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HVH83446.1"
                     /translation="MAWKNQSFPVRLRYALHGIAHALRSEHSLQFQAGAFVAAMVGLA
                     VLRPPPVWWALVLLASALVVGAELLNTALEELADHLNPQEHPRIRLVKDCAAGAVLAA
                     VLGALGVALAFAVQLLHQ"
     gene            3196..4245
                     /gene="bioB"
                     /locus_tag="VM713_03920"
     CDS             3196..4245
                     /gene="bioB"
                     /locus_tag="VM713_03920"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005103519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HVH83447.1"
                     /translation="MSHAAPEPIRHDWTLPEVEALFALPFMDLMFRAQRVHRAWHDPN
                     TVQLSTLLSIKTGACPEDCAYCPQSVHYQTGLARSALLEVAAVRASAERARAAGATRF
                     CMGAAWRQPRARDLKVVAAMIREVRALGLESCATLGMLTAAQAAELKDAGLDYYNHNL
                     DTSAEFYGEIITTRTYQERLDTLDAVRAAGIGVCCGGIVGMGESVTDRARLLRTLANL
                     PEHPGSVPINRLVRVPGTPLADVPDVDPFDFVRAIAVARILMPRAHVRLSAGREAMSD
                     ELQALCLLAGANSIFYGEKLLTTGNPDVERDRQLFARLKVSAERAPGADAAGGDARAA
                     GATSAGHEAGRARAF"
BASE COUNT          580 a         1591 c         1520 g          647 t
ORIGIN      
        1 ccagcagcag gggctggccc gcggcatacg cctgcgcgcc ttcggtgagg cgcgccgcgg
       61 acccctggga ctcgaaatgg tgcacccgga gtaccgccgc ttaggcggcg aggccgcgcc
      121 gctcgaggag accctgacac cgatctatcc gctcaccgag ggggtgccac agggaaggct
      181 gcgcgcgctc atcggccagg cgctcgatga gctcgagcgc tccgggctcg ccgacctgct
      241 gccagcgcgc gcgggactgc cgtcgggatt gccgacgctg cgcgaggcgc tcgcctatct
      301 gcatcgcccg ccgcgtgagg cgcagctcgc cgtgctggcc gccggacgcc acccggcgca
      361 gcggcgcctc gccttcgagg agctgctcgc acacaccctc gcgctgcgct cgcgcaagcg
      421 cgcgttgaag agcgacccgg cctgggagct cgacgaccgg gaaggcctcg cgcgccgtct
      481 catcggcgca ctcccctttg cgctcacgcg ggcccaggcg cgcgcgctcg gcgaggtcga
      541 ggcggacctc gccaccggcg tgccgatggt gcgcctcctg cagggcgatg tgggttgcgg
      601 caagaccgtg atcgcggcgg ccgcggccgc gcgtgccgca gggacgggta agcaggcagc
      661 gctcatggcg ccgaccgagc tcctcgccga gcagcattgg cgcaactttc aggactggtt
      721 tcgcccgctc gggattgcgg tggcgctgct ctcgggcggg caggcggcgc gcacgcgccg
      781 cagcgcactc gagggtctcg cctcggggga gatccgcgtc gtggtcggca cgcacgcgct
      841 ctttcaggaa ggcatcgagt tcgccgctct cgcgctcgtc gtcgtcaacg agcagcatcg
      901 cttcggcgtg cagcagcgcc tcaggctctc ggagaagggg cgggggggcg gccgcctgcc
      961 gcaccagctg atcatgaccg cgacgccgat cccgcgcacg ctcgccatga ccgcctacgc
     1021 cgacctcgac atctcggtga tcgacgagct gccgcccgga cgcacgccgg tcaagaccgt
     1081 ggtggtgccc gaggagcggc gggccgaggt ggtggcgcgc atcgtcggcg cctgcggcgc
     1141 cgggcggcag gtgtactggg tgtgtccgct gatcgacgag tccgacgagc tgcgcgccca
     1201 ggctgcggaa gagacggccg ccgcgctcgc cgaggcactg cccggagtgc gcgtcgggct
     1261 cgtgcacggg cgcatgccgc ctcaacccaa ggatcaggcg atgctcgcgt tcaagtcggg
     1321 gaagatccag ctgctggtcg ccaccaccgt gatcgaggtg ggcgtcgatg tccccaacgc
     1381 caccctcatg gtcatcgaga acgccgagcg catgggactc gcccagctgc accagctgcg
     1441 cggacgcgtc ggccgcggcg ccgaggtgag cacctgcgtg ctgctctatc gcccaccgct
     1501 ctcggagccg gcgcacgagc ggctcaaggc catccgcgcg accaacgacg gcttcgagat
     1561 cgcgcggcgt gacctcgagc tgcgcggacc gggcgagctc ctcggcacgc gccagaccgg
     1621 gctcgcgcag ctgcgcgtcg ccgacctgat gcgtgatgcg gatctcctgc ccgccgtgca
     1681 ggcggccgcg cagctgctcg agtcgcaccc ggagagcagc gccgcgctga ccgcgcgctg
     1741 gatcggcagc ggcgagcgct acgggcgcgt gggctaacat actcaccacg catggcagcc
     1801 gcagcccccg aacttcaggc gacgacgccg gcgccgggtc ttgccgagac acccaccccg
     1861 gcgccgcgct ttcgcgaggc cccgctctcg tggcggctca cgcgtcgcct cactcagtac
     1921 gggcgcctca tgcggctcga tcgcccggtg gggacgtggc tgctcctgtg gccggcgctc
     1981 tgggcgctgt gggtcgcggg cgctgcgcgg ccgaagccca acgttctcat catattcgtg
     2041 ctcggcgtcg tggtgatgcg cgccgcgggc tgcgtgatca acgacttcac cgaccgcgac
     2101 ctcgacccct acgtgcggcg cacgcgcgac cgcccgctcg ccgcgcgtct cgtgacaccg
     2161 gtcgaggcgc tcttcctgtt cgcggtgctg atcgcggtcg cgctctatct ggtgacgcgg
     2221 ctcgatgagt tcaccgtgaa gctcgccgcc atcggcgcgc tcctcacgat ctcctacccg
     2281 ttcgtcaagc gcgtcttccc catgccgcag ctctacctcg gcatctcctt cggcggctgg
     2341 gccgtgccga tggccttcgc ggcgcagctc ggtacgctgc cgcgggtgtg ctgggtgctg
     2401 tacatcgccg cggtgatctg ggccgccatg tacgacacga tctacgccat gatcgaccgc
     2461 gaggacgaca tcaagatcgg cgtgaagtcg agcgccatcc tgttcggcga catggacagg
     2521 ctcctgatcg gcgtgatgca gctgatgatg ctgtacgcgc tctacctcgt cggcgaggac
     2581 atgcactaca gccgctggta ccggggcggc gtgatcgccg ccgcggagtt gttcctctgg
     2641 cagcaatggc tcatccgccg gcgcgacccg gccgggtgcc tgcgggcctt cctcaataac
     2701 cagtacgtcg ggctcgtgat cttcgcaggt ctgctgctgc agtacgtgta caagagctga
     2761 gccgcgcgcc gtggcctgga agaatcagtc attcccggtg cgcttgaggt acgcgctcca
     2821 cggcatcgcg catgcgctgc gcagcgagca cagcctgcag tttcaggccg gggcattcgt
     2881 ggcggcgatg gtggggctcg ccgtgctgcg cccgcccccc gtgtggtggg cgctggtact
     2941 gctcgccagc gcgctggtcg tcggggccga gcttctcaac acggctctcg aggagctcgc
     3001 cgaccacctc aacccgcagg agcatccgcg catccgcctg gtcaaggact gcgccgccgg
     3061 cgcggtgctg gcggcagtgc tcggggcgct cggcgtggcg ctcgccttcg cggtacagct
     3121 gctccatcag taattgtcga ctttggatcg cccgtcgcgt tgacacgggg cagcagcctc
     3181 gggcaaggtc accgcatgag ccacgccgcg cccgagccga tccgccacga ctggacgttg
     3241 cccgaggtgg aggcgctctt cgcactgccc ttcatggatc tcatgttccg cgcgcagcgg
     3301 gtgcaccgcg cctggcacga tccgaacacg gtgcagctct cgaccctgct ttccatcaag
     3361 accggcgcct gccccgagga ctgcgcctac tgcccgcaga gcgtccacta ccagaccggc
     3421 ctcgcgcgct cagcgctcct cgaggtcgcc gcggtacgcg cgagcgccga gcgggcgcgc
     3481 gccgccggcg cgacccgctt ctgcatgggc gccgcctggc gccagcccag ggcgcgcgac
     3541 ctgaaggtcg tcgccgccat gatccgcgag gtgcgcgcgc tcgggctcga gagctgcgcg
     3601 acgctcggca tgctcaccgc cgcgcaggcc gcggagctca aggatgcggg cctcgactac
     3661 tacaatcaca acctcgacac ctcggcggag ttctacggcg agatcatcac gacgcgcacc
     3721 taccaggagc ggctcgacac gctggacgcg gtgcgcgcgg ccggcatcgg cgtgtgctgc
     3781 ggcggcatcg tcggcatggg cgagtcggtc accgaccgcg cgcggctgct gcgcacgctc
     3841 gccaacctgc cggagcatcc gggcagcgtc cccatcaacc gcctggtcag ggtgccggga
     3901 acgccgctcg ccgacgtacc ggacgtcgat cccttcgact tcgtgcgcgc gattgcggtc
     3961 gcgcgcatcc tcatgccgcg cgcccacgtg cgcctctccg ccgggcgcga ggcgatgtcg
     4021 gacgagctgc aggcgctgtg cctgctcgcg ggggcgaatt ccatcttcta cggcgagaag
     4081 ctcctcacca ccggcaaccc cgacgtcgag cgcgaccggc agctcttcgc acgcctcaag
     4141 gtcagcgccg agcgagcccc gggcgctgac gcggccggag gcgacgcccg cgccgcgggt
     4201 gcgacgtccg ccggccatga agcgggccgc gcacgcgctt tctgaggcgc tgtcgcggct
     4261 cgaagccgcg cagctcaggc gccgccgcgc caccgtcgag gggttcgcgg ctgccgacca
     4321 ccgtgcgcac gtagtggt
//
LOCUS       DATLKL010000300         2588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_14482, whole genome shotgun sequence.
ACCESSION   DATLKL010000300 DATLKL010000000
VERSION     DATLKL010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14482"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VM713_03925"
     CDS             <1..404
                     /locus_tag="VM713_03925"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HVH83448.1"
                     /translation="PGGTPAGGRLEVTVEVAALDTGDKDRDDTLRSDDLFAVAKFPQA
                     RFSAPQVVKTASGYEAAGKLTIRGVTRDANVPFTFREATEGGAAVAYMTGKTVVRRLD
                     FGVGQGDWKATDQAGNEVTVSFALRLARAPR"
     gene            complement(406..882)
                     /locus_tag="VM713_03930"
     CDS             complement(406..882)
                     /locus_tag="VM713_03930"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVH83449.1"
                     /translation="MASHQGAVEARSVAAQSRSNYPEPYRSRVLPRARRRLGDVFGLT
                     RIGINQTTLSPGKESSMRHWHTHEDEFLYVLEGEAVLRTDAGEQLLTAGMCVGFPAGV
                     ANGHQLINRSDRPVVYLEISNRDPEDSAYYADPDVDMVWSAPQARGRISRRDGRPY"
     gene            953..>2588
                     /locus_tag="VM713_03935"
     CDS             953..>2588
                     /locus_tag="VM713_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HVH83450.1"
                     /translation="MPEIFDDVGHCVDAVLRRVGPRVVLALPLGIGKPNHLANEFYRR
                     AARDPGLELTIVTALSLLKPVAHSALEGRLLAPLVTRVFGSYVEPEYARAMRADRLPG
                     NVRVIEFFLTPGAFLGASHAQRNYLSANYTHVAREVMAHGVNVIAQLVARRTVNGQTL
                     LSFGSNADVTPDLLPQIAAARRAGREILMVGEIHAQMPFMTGHAQVEPERFDFLIDDP
                     RYDFDLFGPPNPPLSSVDHAIGMYASSLVCDGGTLQIGIGELGDSLAYALLLRHQQNA
                     AWASALGALGTRTAADLIRATGGLEPFARGLFASTEMFVDQLLELYHAGILRRRVYDS
                     LPLERLLAAGALAERFDAGLLERLVAAGVGPTLSATEFGELKHYGVFREDVEFAGGRV
                     RAPGGEWIRADLSDAQCRAALAQCLGRELRNGQVLHAGFFLGPRGFYAALRELPESDR
                     AQLGMRGVAYVNQLYGPDQELRILQRRGSRFVNTTMMVTLLGAAVSDALESGQVVSGV
                     GGQYNFVAMAHALPDARSILCVRATRTRHGRTTSNIV"
BASE COUNT          374 a          923 c          879 g          412 t
ORIGIN      
        1 acccgggtgg gacgccggcc ggcgggcggc tcgaggtcac ggtagaggtc gcggcgctcg
       61 ataccgggga caaggatcgt gacgacacgc tgcgcagcga tgacctgttc gcggtggcga
      121 agtttccgca ggcacgtttc agcgccccgc aggtcgtgaa gacggccagc ggttacgaag
      181 ccgccggcaa gctcacgatc cgcggtgtga cccgcgatgc aaacgtgccc tttacgttcc
      241 gcgaggccac cgaaggcggc gccgcggtcg cctacatgac cggcaagacg gtcgtgcgcc
      301 gcctcgactt cggagtcggt cagggtgact ggaaagccac cgaccaggcc gggaacgagg
      361 tcaccgtctc gttcgcgtta cgcctcgcgc gggcgccccg gtagatcagt acggccttcc
      421 gtcgcggcgg ctgatccggc cgcgcgcctg cggcgcgctc cacaccatat cgacgtccgg
      481 atcggcgtag taggccgagt cctcggggtc gcggttgctg atctcgaggt agacgaccgg
      541 gcggtcgctg cggttgatga gctggtgtcc gttggcgacg cccgcgggaa aaccgacgca
      601 catgccggcg gtgagcaact gctcgccggc gtcggtgcgc agcaccgcct cgccctcgag
      661 cacgtacagg aactcgtcct cgtgggtatg ccagtggcgc atcgaggact ccttcccggg
      721 cgagagcgtc gtctggttga tgccgatgcg cgtcaagccg aagacatcgc ccagacggcg
      781 cctcgcccgc ggcaggaccc gcgaccggta gggctcggga tagttgctgc ggctctgcgc
      841 cgcgacgctg cgcgcctcga cggcgccctg gtgcgatgcc atgtcacgct cctcatggtg
      901 tgcgcgcggt tgacgcgcat cagttccatc ggggagcatg ccacaagctc gcatgcctga
      961 aatattcgac gacgtcggac actgcgtcga cgcggtgctg cgccgcgtgg gaccacgcgt
     1021 cgtgctggcg ctgccactcg gcatcggcaa gcccaaccac ctcgccaacg agttctaccg
     1081 gcgcgccgcg cgcgatcccg ggctcgagct caccatcgtc acggcgctgt cgctgttaaa
     1141 gcccgtggcg cactccgccc tcgaggggcg tctgctcgcc ccgctcgtca cgcgcgtgtt
     1201 cggcagctat gtggagccgg aatatgcgcg cgccatgcgc gccgaccgcc tgcccggcaa
     1261 cgtccgggtc atcgagttct tcctgacccc cggcgcgttt ctgggcgcct cccacgcaca
     1321 gcggaattat ctgagcgcca attacaccca cgtcgcccgc gaggtcatgg cgcacggcgt
     1381 caacgtgatc gcacagctcg tggcgcgacg caccgtgaac gggcagaccc tcttgagctt
     1441 cggctcgaac gccgacgtga cgcccgacct gctgccgcag atcgcagcgg cacggcgcgc
     1501 cgggcgtgaa atcctcatgg taggtgagat tcacgctcag atgcccttca tgaccgggca
     1561 cgcgcaggtc gagcccgagc gcttcgactt cctcatcgac gacccgcgtt acgacttcga
     1621 cctcttcggc ccgcccaatc cgcccctcag cagcgtcgat cacgccattg gcatgtacgc
     1681 gagcagcctg gtgtgcgacg gcggcactct gcagatcggt atcggcgagc tcggcgactc
     1741 gctcgcctac gccctgttgc tgcggcacca gcagaacgcg gcctgggcga gcgcactcgg
     1801 cgccctcggg acgcgcaccg ccgccgacct catccgcgcc acgggcggac tcgagccctt
     1861 cgcgcgcgga ctgttcgcct ccaccgagat gttcgtcgat cagctgctcg agctctacca
     1921 tgccggcatc ctgcgccggc gcgtctacga ctcgctgccg ctcgagcggc tgctcgccgc
     1981 gggggcgctc gccgagcgct tcgatgccgg gcttctcgag cggctggtcg ccgccggcgt
     2041 gggtccgact ctgagcgcga ccgagttcgg cgaactcaag cactacgggg tgttccgcga
     2101 ggacgtggaa ttcgccggcg ggcgggtgcg cgccccgggc ggcgagtgga tccgcgccga
     2161 cctgagcgac gcgcagtgcc gcgcggcgct cgcccaatgt cttggacgcg agctgcgtaa
     2221 cggccaggtc ctgcacgccg ggttcttcct cgggccgcgc ggcttctacg cggcgctgcg
     2281 cgagctgccc gagagcgacc gcgcgcagct cggcatgcgc ggcgtcgcct acgtcaacca
     2341 gctgtacggt cccgaccagg agctgcgcat cctgcagcgg cggggctcgc gcttcgtcaa
     2401 caccacgatg atggtaacgc tgctcggggc ggcggtctcg gatgcgctcg agagcggcca
     2461 ggtggtgtcg ggcgtcggcg gtcaatataa cttcgtcgcc atggcgcatg cgctacccga
     2521 cgcgcgctcg atcctgtgcg tgcgcgcgac gcgcacgcgc cacggccgca ccacctccaa
     2581 tatcgtct
//
LOCUS       DATLKL010000301         1766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_104886, whole genome shotgun sequence.
ACCESSION   DATLKL010000301 DATLKL010000000
VERSION     DATLKL010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104886"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..>1766
                     /locus_tag="VM713_03940"
     CDS             40..>1766
                     /locus_tag="VM713_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HVH83451.1"
                     /translation="MLSSSPVDPRKTHAVFGGVAEEAAARKKAAHAAQLAHTAHAEPA
                     TPPTPAAADDFDQEPTLSAAALQVIGMNGAKSRTLLIAGACLAVAVLAAGAWFLTRGP
                     GTAPVQPSAVVREAPVAAPVTAPAAEGPESTLPLAPSADLAILHGKVDELLEKARLAM
                     RERRYTEPIGDNALVYYRSAVAADATNGEARDGLQRVAAVIAGRLDESLNAGRLDEAA
                     LNLASLKAAAPDDRRTTEFAARLAAAQFTKALAEGSLERAAGFLRQAQQSAAVPADQL
                     AKWKSDLARRQDDVKTVLRLAGLVEDRIRDGKLVDPAGDSAKAYLGQLLSAYPSSLSA
                     QRVQRELTSAYLRKAHDAGVARNSAEAERWLNEARAAGAAPADISTLQRDLAGAQAKA
                     VRSEGDRLAQLARERVRDGRLTDPAQDSAAYYLTQLQSADPDNAALAAVSRDLAGKLL
                     DRARTATQAGRSADLDLSAARRFGADPKDILGAQQSGAKGTNVDPATLAAGLKALRTE
                     PPEYPAGALEHRMTGSVLLSYTVDGKGNPRDVQILQSTPTGVFDRAAMNAVKRWKYAP
                     VIVNGAPVEV"
BASE COUNT          287 a          651 c          609 g          219 t
ORIGIN      
        1 caggtggccg cgtcggtcaa aggctcgaag gttttcgcag tgctgtcctc ctcaccggtc
       61 gacccgcgca agacccatgc ggtgttcggc ggagtagctg aggaggccgc ggccagaaag
      121 aaagcggccc acgcggcgca gctcgcgcac acggcccacg cggaacctgc cactcccccg
      181 acgcccgccg cggccgacga tttcgaccag gaaccgacgc tcagcgcagc cgccctccag
      241 gtcatcggga tgaacggcgc gaaaagccgg acactgctga tcgcaggcgc ttgcttggcc
      301 gtggccgtac tggctgccgg cgcgtggttc ttgacccgtg gccccggcac cgcgccggtt
      361 cagccttccg ccgtagtccg ggaggcgccg gtcgcggcgc ccgtcacagc gcccgcggcc
      421 gaaggccccg aatcgacgct cccgctcgcc cccagtgcag atctcgcgat cctgcacggc
      481 aaggtggacg agcttctgga gaaggctcgt ctcgccatgc gcgagcggcg ctacaccgag
      541 cccatcggcg acaatgcgct ggtctactac cggtccgccg tcgcggccga cgccaccaac
      601 ggcgaggccc gggatggcct gcagcgcgtc gccgcggtaa tcgccggccg gctggatgaa
      661 tctctgaacg ccggtcgcct ggacgaggcg gcgctgaatc tcgccagctt aaaggcggcg
      721 gcacccgacg atcggcgcac cacggagttt gccgcgcggc ttgccgccgc gcagttcacc
      781 aaggcactcg ccgaagggag cctggagcgc gcggcggggt tcctccgtca ggcacagcag
      841 tccgcggccg tgcccgccga tcagctcgcc aagtggaaga gcgacctcgc gcggcgtcag
      901 gacgatgtca agaccgtgct gcgcctcgcg ggtctcgtgg aggaccgcat ccgcgacggc
      961 aagctcgtcg accctgcggg tgacagcgcc aaagcctacc tcgggcagct tctctcggcc
     1021 tatccgtcga gcctgagcgc gcaacgcgtc cagcgcgagc tcaccagcgc ctacctgcgc
     1081 aaggcgcacg acgccggggt cgcccgcaac agcgccgagg cggagcgctg gctcaacgag
     1141 gcgcgcgccg ccggcgcggc gcccgccgac atcagcaccc tccagcgcga cctcgccggc
     1201 gcgcaggcca aggcggtccg cagcgagggc gacaggctcg cgcagctcgc gcgcgagcgc
     1261 gtacgcgacg gacgcctcac cgatcccgcc caggacagcg ccgcgtacta cctgacgcag
     1321 ctgcagtcgg ccgatccgga caacgcggca ctcgctgcgg tcagtcgtga tctcgccggg
     1381 aagctcctcg accgggcacg caccgcgacg caggccggca ggagcgccga cctggacctg
     1441 agcgctgcgc gccgcttcgg cgcggacccg aaggacatcc tgggcgcgca gcagtccggg
     1501 gcaaagggaa cgaatgtcga tccggcgact ctggccgcag gcctcaaagc gctgcggacc
     1561 gagccgccgg agtatccggc gggcgccctc gagcaccgca tgaccggcag tgtgctgctg
     1621 tcgtacacag tcgatggcaa gggcaacccc cgcgacgtgc agatcctgca gtcgaccccg
     1681 accggtgtgt tcgatcgcgc ggcaatgaac gccgtgaagc gttggaagta cgcgccggtg
     1741 atagtcaacg gcgccccggt cgaggt
//
LOCUS       DATLKL010000302         2077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_150147, whole genome shotgun sequence.
ACCESSION   DATLKL010000302 DATLKL010000000
VERSION     DATLKL010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150147"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VM713_03945"
     CDS             complement(<1..981)
                     /locus_tag="VM713_03945"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVH83452.1"
                     /translation="MQVEITVLPNGLRVVSDCMEGVETVSLGVWVGAGTRFESPELNG
                     VSHLLEHMAFKGTRRRSAQAIAEEIEAVGGHLNAYTTREHTAYYAKVLKEDLGLALDI
                     IADILQHAAMDGEELARERAVVLQEIGQANDTPDDIIFDYFQETAFPGQPLGRPVLGS
                     AKVVEGMPREALLAYMAHHYGGARMVLAASGRLDHEELVARAAEAFRELPATAPSAPE
                     GARYVGGDFRADRGLEQVHLVIGLEGLSYHDPDFYALQVFSTLLGGGMSSRLFQNIRE
                     KQGLAYAVFSELTPYSDAGILSVYAGTAKETVGQVIDLTIAEFRAMKESPV"
     gene            complement(1139..2017)
                     /gene="thrC"
                     /locus_tag="VM713_03950"
     CDS             complement(1139..2017)
                     /gene="thrC"
                     /locus_tag="VM713_03950"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVH83453.1"
                     /translation="MTTVSAANVWNIAIDGSFDDCQDLVKAMFSDQPFRDAMRLSAVN
                     SINWARIMAQIPYYVWSALALGAPERPVAFAVPSGNFGNVYAGYAAREMGLPIAQLVV
                     GSNRNDVLARFFEQGAIETRAVVPTLSPSMDIQIPSNFERLLFDLLGRDGGKVASAMD
                     RLRAGGRLAIAKPAWRRARRLFSGHCLDDEATRRAITDVYRDTGVLLDPHTAIGVMAG
                     RAARRDAAIPVIALATAHPAKFPDAVEAATGVRPALPARLADLLARPERFTSLPNQLG
                     AVEAFIRERVSLRGAA"
BASE COUNT          364 a          693 c          689 g          331 t
ORIGIN      
        1 caccggggat tccttcatgg cgcgaaattc ggcgatggtg agatcaatca cctggccgac
       61 ggtttccttc gcggtgcccg cgtaaacact caaaatgccg gcgtcggaat agggagtcag
      121 ttcgctgaag accgcgtagg cgaggccctg cttttctcga atgttctgga agaggcgtga
      181 ggacatgccg ccgccgagca gcgtggagaa cacttgcagc gcgtagaagt cggggtcgtg
      241 gtaggagagc ccctcgagcc cgatcaccag atggacctgc tcaagcccgc ggtcggcgcg
      301 gaagtcgccc cccacgtaac gggccccctc gggcgccgat ggcgccgtcg ccggcagctc
      361 gcggaacgcc tcggccgcgc gggccacgag ctcctcatgg tcgagcctgc cggatgccgc
      421 caataccatg cgcgcgccgc catagtggtg cgccatataa gcgagcaggg cctcgcgcgg
      481 catgccctcc accaccttgg ccgagcccag cacggggcgc ccgagcggct ggccggggaa
      541 agcggtctcc tggaagtagt cgaagatgat gtcgtccggt gtgtcgttcg cttggccgat
      601 ctcctgcaac acgaccgccc gctcgcgcgc cagctcctca ccgtccatgg ccgcgtgctg
      661 caagatgtcg gcgatgatgt cgagagccag cccaaggtcc tccttgagca ccttggcgta
      721 ataagcggtg tgctcgcgcg tcgtgtaggc gttgagatgg ccgcccacgg cctcgatctc
      781 ctcggcgatc gcctgcgccg agcgccgccg cgtccccttg aaggccatgt gctcgaggag
      841 atgggaaacg ccgttgagct cgggcgactc gaagcgggtg cctgccccca cccacacgcc
      901 caaggacacc gtctcgaccc cctccatgca gtcgctcacc acccgcaagc cattgggaag
      961 aacggtaatt tcgacctgca ttgcgggccc gctcggggcc cccggcacca ccggacgcaa
     1021 cccagccatt ttctttaatt tcctttcaaa tcaaggcctt aggaaatagg gggcccggcc
     1081 cacgccccat ataatatgat ccccaagagg ccgagatgta caggcgaatt gaggggtgtc
     1141 acgccgctcc gcgcaggctc acgcgctcgc gaatgaaggc ctcgaccgcg ccaagctggt
     1201 tgggaagcga ggtgaaccgc tccggcctgg cgaggaggtc ggcgagccgc gcaggcagcg
     1261 ccgggcggac cccggttgca gcctcgaccg cgtcgggaaa cttggcggga tgcgcggtcg
     1321 cgagcgcgat cacagggatc gccgcgtcgc ggcgcgcggc ccggcctgcc atgaccccga
     1381 tcgccgtatg gggatcgagg agcacgcctg tgtcgcggta gacgtctgtg atcgcacgcc
     1441 tggtcgcctc gtcgtcgagg cagtggcccg agaagagtcg ccgcgccctt cgccacgccg
     1501 gcttggctat cgcgaggcgg ccgccggccc gcagacggtc catggcagag gccaccttgc
     1561 cgccgtcgcg gccgagcagg tcgaagagca ggcgctcgaa gttgctcggg atctgaatgt
     1621 ccatgctcgg cgagagcgtc ggcacgaccg ctcgggtctc gatcgcgcct tgttcgaaga
     1681 accgggccaa aacgtcgttg cggttggaac cgaccacgag ctgagcaatc ggcagtccca
     1741 tctcccgcgc ggcgtagccg gcatagacgt tgccgaaatt tccggaaggc acggcaaagg
     1801 cgacggggcg ctcgggcgcc cccaacgcca gcgcggacca gacgtaatag ggaatctggg
     1861 ccatgatgcg cgcccaattg atcgagttga ctgccgagag ccgcatcgcg tcacggaagg
     1921 gctggtcgct gaacatcgcc ttcaccaagt cctggcagtc gtcgaagctt ccgtcgatcg
     1981 cgatgttcca cacattggct gctgacaccg tggtcatctg gcgccgctgc acctccgaca
     2041 cgcggccgcg gggatggagg atgaggattt cgatcgc
//
LOCUS       DATLKL010000303         2348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_37275, whole genome shotgun sequence.
ACCESSION   DATLKL010000303 DATLKL010000000
VERSION     DATLKL010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37275"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /gene="paaA"
                     /locus_tag="VM713_03955"
     CDS             <1..650
                     /gene="paaA"
                     /locus_tag="VM713_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HVH83454.1"
                     /translation="QLLSGVAKYSSIFNYPTLTWADIGAIGWLVDGAAIMNQIPICRC
                     SYGPYARAMVRICKEESFHQRQGFEIMLTLARGSAAQRAMAQDALNRWWWPSIMMFGP
                     SDSDSKHTAQSMRWKIKRFSNDELRQKFIDITVPQAQFLELTIPDAALSWDEATQHFS
                     IGPIDWDEFHAVLKGNGPCNRERLEARRAAYRAGAWVRAAAAAHAAKEAAREREV"
     gene            647..955
                     /gene="paaB"
                     /locus_tag="VM713_03960"
     CDS             647..955
                     /gene="paaB"
                     /locus_tag="VM713_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HVH83455.1"
                     /translation="MSTPTPDPATGDWPLFEVFIRSRSGLEHKHVGSLHAADARMAIE
                     HARDVYTRRQEGVSIWVVRSAEIVASDPAESDALFEPARDKVYRHPTFYAIPDEVKNL
                     "
     gene            952..1746
                     /gene="paaC"
                     /locus_tag="VM713_03965"
     CDS             952..1746
                     /gene="paaC"
                     /locus_tag="VM713_03965"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HVH83456.1"
                     /translation="MSARAAPPARRDQALAEALLRYVLRLADTSLVLGQRLGEWIGHA
                     PALEEDLGLANLALDLIGQARLLLTYAGEIEGRGRDEDALAYFRDAPEFLNLTLAEQP
                     NGDFAQTIVRQWLIDAWQLELYEGLTGSSDTRLAAIAAKALKETRYHYRFSSGWLVRL
                     GDGTTESQARTQQAVDSLWRFTEELFTPDDADLAVAAAGIAPQLAAAGGRFRARLDDD
                     LRRATLVRPSGQPHPWYGKRGVHTEHLGHLLGEMQHLQRTYPGARW"
     gene            1761..2240
                     /gene="paaD"
                     /locus_tag="VM713_03970"
     CDS             1761..2240
                     /gene="paaD"
                     /locus_tag="VM713_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HVH83457.1"
                     /translation="MAADRGGLQTPAEDALWELLARVPDPEIPVLSIVDLGVVRYVRR
                     AADGALHIGITPTYTGCPATAAIRSATRAALDAAGFSAVLLEEVLSPPWTSDWVTPAG
                     RAKLLAFGIAPPAAAPVRWPRCGSPATERISEFGSTPCKAHYVCSACREPFDHFKCI"
BASE COUNT          357 a          823 c          814 g          354 t
ORIGIN      
        1 agcaactgct gtcgggtgtc gccaagtact cgagcatctt caactacccg acgctcacct
       61 gggcagacat cggcgccatc ggctggctgg tggacggcgc ggcgatcatg aaccagatcc
      121 cgatctgccg ctgctcgtac gggccttacg cccgcgccat ggtgcgcatc tgcaaggagg
      181 agagcttcca ccagcgccag ggcttcgaga tcatgctgac gctcgcgcgc gggagcgccg
      241 cgcagcgcgc catggcgcag gatgcgctca accgctggtg gtggccgtcg atcatgatgt
      301 tcgggccgtc ggactctgac tccaagcaca ccgcccagtc gatgcgctgg aagatcaagc
      361 gcttcagcaa cgacgagctg cggcagaaat tcatcgatat caccgtgccg caggcgcagt
      421 tcctcgagct gactattccg gacgcggcac tgagctggga cgaggccacg cagcacttct
      481 ccatcggccc gatcgactgg gacgagtttc atgcggtcct caaaggcaac ggtccgtgca
      541 accgcgagcg gctcgaggcg cgacgcgcgg cctaccgggc cggcgcctgg gtgcgcgccg
      601 ccgccgcggc gcatgccgcc aaagaggcgg cacgggagcg cgaggtatga gcactccgac
      661 tcccgacccg gccacgggcg actggccgct gttcgaggtg ttcatccgct cgcgctccgg
      721 gctcgagcac aagcacgtcg gcagcctcca cgcggcggat gcgcgcatgg cgatcgagca
      781 cgcgcgcgac gtgtacacgc gccgccagga gggcgtgtcg atctgggtgg tgcgctccgc
      841 cgagatcgtc gcctcggatc ctgccgagtc ggacgcgctg ttcgagccgg cgcgtgacaa
      901 ggtgtaccgg cacccgacct tctatgcgat cccagacgag gtgaagaatc tgtgagcgcg
      961 cgcgccgcac cgcccgcgcg gcgcgaccag gcgctcgccg aggcgctgtt gcgctacgtg
     1021 ctgcgcctcg ccgatacgag cctcgtgctc ggccagcgcc tcggagagtg gatcgggcac
     1081 gcgccggcgc tcgaggagga tctggggctc gccaacctgg cgctcgacct catcggccag
     1141 gcgcggctgt tgctcaccta cgccggggag atcgagggcc ggggacgcga cgaggacgcg
     1201 ctcgcgtact tccgcgatgc cccggagttc ctgaatctga cgctcgccga gcagccgaac
     1261 ggcgacttcg cccagaccat cgtgcgccag tggctcatcg atgcctggca gctcgagctt
     1321 tacgagggcc tcacgggctc gagcgatacg cggctcgcgg cgatagccgc caaggcgctc
     1381 aaggagacgc gctatcacta ccgcttcagc agcggctggc tggtgcgcct cggcgacgga
     1441 accacggaga gtcaggcgcg cacgcagcag gcggtggact cgctgtggcg tttcaccgag
     1501 gagctgttca ctcccgacga cgccgacttg gcggtggccg ctgcgggcat cgcgccgcag
     1561 ctcgcggcag ccggcggccg cttccgtgcg cgtctcgacg acgacctgcg ccgggcgacg
     1621 ctggtgcgtc cctcggggca gcctcatccc tggtacggca agcgcggcgt gcacaccgag
     1681 cacctcggcc acctgctcgg ggagatgcag catctgcagc ggacctaccc tggggcgcgc
     1741 tggtgagagc ggagtccagc gtggcggcgg acagaggcgg cctgcagact cccgccgagg
     1801 acgcgctgtg ggagttgcta gcacgcgtac ccgatcccga gattccggtg ttgagcatcg
     1861 tcgacctggg tgtcgtgcgc tacgtgcgcc gcgccgccga cggagcgctg cacatcggca
     1921 tcactccgac ctacaccggt tgtccggcga ccgccgcgat ccgcagcgcg acgcgcgccg
     1981 cgctcgatgc cgccgggttt tccgccgtgc tcctcgagga agttctctcc ccgccctgga
     2041 cgagcgactg ggtgacgccc gcggggcgcg ccaagctcct ggctttcggc atcgccccgc
     2101 ctgcagcggc gccggtgcgt tggccgaggt gcggcagccc cgcgaccgag cggatcagcg
     2161 aattcggttc gacgccctgc aaggcgcact acgtctgcag cgcctgccgc gagcctttcg
     2221 atcacttcaa gtgcatctga catgctgcgc ttccattcac tcacggttgg cgccatcagc
     2281 ccggatgcgg aggacgccgt ggccatcgcg ctcgaggtgc cggcgacgct gcgcgacgag
     2341 taccgcgg
//
LOCUS       DATLKL010000304         2406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_161274, whole genome shotgun sequence.
ACCESSION   DATLKL010000304 DATLKL010000000
VERSION     DATLKL010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161274"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2406)
                     /gene="mprF"
                     /locus_tag="VM713_03975"
     CDS             complement(<1..>2406)
                     /gene="mprF"
                     /locus_tag="VM713_03975"
                     /inference="COORDINATES: protein motif:HMM:NF033480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HVH83458.1"
                     /translation="RRWLGPVAALLVFAAVVLILHRQLAHLHVRSVLEHLHSIPRRQV
                     LAALGFTAASYWLLSNYDVLALAYLRRAMPYPRILFTSFIAYSFGHTLGFAAFTGAAI
                     RFRLYATAGVSAIDVATISAFCSLSLGIGLATIAGLSLFLSPAHASQILHLHHNWSLL
                     AGAVLLGAVALYALWATLARSTLELRGWALRAPGPAIGLAQLALSVVDLSLACAVLWS
                     LLPAGAHIGFVPFLGTYALAVIAGIVSHVPGGVGVFEAVMLLTLPGVPADALLGSLLA
                     YRGVYYLVPLVFGTLLFGSKELAAQRSRMLRAQELASVYIAPVVPQVAGALTFLAGAL
                     LLFSGATPALEERLAFLDRFLPLAVLEVSHLAGSVIGLGLLVLSRALFRRVQGAYHIA
                     VWLLVAGIFASLLKGFDFEEATLLALVLGVLVFGRRAFYRPTAILSERFTPAWVASIA
                     GVIALAIWLGVLTNRHVAYSRELWWTFALDGNAPRMLRASLSIVLLGTAYVLLNMLRP
                     ARPEPAVAGPAELARARALIEQSDNTVANAALTGDKRLLFNEAGTAFVMYQIAGRSWI
                     ALGDPVGARAGAEELVWRLREMSDRHGCQTVFYQVGGDRLALYVDLGLAALKIGEEAR
                     VPLEQFTLEGAARADLRQAYRRAQRDGASFEVVPPEGVAALLPALQRISDAWLASKST
                     GEKRFSVGAFSAQYLTQFPLAVVRAEGTPVAFANLWTTGTKMELSVDLMRFGPEAPRS
                     AMDYLFIELMLWGQRTGYRYYGACACRMRPSSRCRESPPPGGARHRATAPPAPARRRR
                     SSP"
BASE COUNT          443 a          830 c          872 g          261 t
ORIGIN      
        1 gtggagaaga acgtcgcctt cgggctggag caggaggggc tgtcgcgcga tgccgtgcgc
       61 cgccaggtgg gggagattct cgacatcgtg aagatgggcg cattcggcac gcgcaagccc
      121 catagtagcg atagccggta cgctgtcccc acagcatgag ctcgatgaac aggtaatcca
      181 tcgcactgcg cggggcctcg gggccgaagc gcatcagatc gacggacagc tccatcttcg
      241 tccccgtcgt ccagaggttg gcgaaggcca ccggcgtgcc ctcggcccgc accaccgcca
      301 gcggaaactg cgtcaggtac tgcgctgaga acgcgcccac cgagaagcgc ttctcgccgg
      361 tcgatttgct cgcgagccag gcgtcggaga tgcgctgcag cgccggcagc agcgccgcga
      421 cgccctccgg cggcacgacc tcgaagctcg ccccgtcgcg ctgcgcgcgc cggtacgcct
      481 ggcgcaggtc cgcgcgcgct gcgccctcga gcgtgaactg ctcgagcggt acacgcgcct
      541 cctcgccgat cttgagcgcg gccaaaccta aatcgacata gagcgcgaga cggtcgccgc
      601 ctacctgata gaagacggtc tggcagccgt gacggtcgga catctcgcgc agccgccaca
      661 cgagctcctc ggcccccgcg cgcgctccca ccggatcgcc gagcgcgatc cagctgcgac
      721 ccgcgatctg gtacatcacg aaggcggtgc cggcctcgtt gaacagcagc cgcttgtcac
      781 ccgtgagcgc cgcgttcgcc accgtgttgt cggactgctc gatcagcgcg cgcgcccgcg
      841 cgagctccgc gggtccggcg acggcaggct cggggcgtgc cggccgcagc atattgagca
      901 gcacgtaggc ggtgccgagc agcacgatcg agagcgaggc gcgcagcatc cgcggcgcat
      961 tgccatcgag cgcgaacgtc caccacagct cgcgggaata ggcgacgtgc cggttggtga
     1021 ggaccccgag ccagatcgcg agcgcgatca ccccggctat gctcgccacc cacgccggcg
     1081 tgaagcgctc ggagaggatc gcggtcgggc ggtagaaggc gcgccggccg aacaccagca
     1141 cgccgagcac gagagcgagc aacgtcgcct cctcgaagtc gaagcccttg aggagcgagg
     1201 cgaagatgcc cgcgaccagc agccacaccg cgatgtggta agcgccctgc acgcggcgga
     1261 acagcgcgcg cgagagcacc aggagcccga gaccgatcac gctgccggcg aggtgcgaga
     1321 cctcgagcac cgcgagcggc aggaagcggt cgaggaatgc gagccgctcc tcgagggccg
     1381 gggtggcgcc cgagaacagg agcagcgctc cggcgaggaa ggtgagcgcg cccgccacct
     1441 gcggcacgac cggcgcgatg tagacagagg cgagctcctg cgcccgcagc atgcggctgc
     1501 gctgcgcggc gagctccttc gagccgaaca gcagcgtccc gaacacgagt ggcaccaggt
     1561 agtacacgcc ccggtaggcg agcagcgacc ccagcagcgc gtccgccggc accccgggaa
     1621 gggtgagcag catcaccgcc tcgaacactc ccaccccgcc cgggacgtgg ctcacgatcc
     1681 cggcgatcac cgcgagcgcg taggtgccga gaaacggcac gaagccgatg tgcgcccccg
     1741 ccggcagcag cgaccacagc accgcgcagg cgagcgacag atccaccacc gagagggcga
     1801 gctgcgccag cccgatcgca ggtcccgggg cgcgcagcgc ccagccgcgc agctcgagcg
     1861 tgctgcgcgc gagggtcgcc cacagggcgt agagagccac ggcgccgagc agcaccgcgc
     1921 cggcgaggag cgaccagttg tgatgcaggt gcaggatctg cgaggcgtgc gcgggcgaga
     1981 ggaacagcga gagccccgcg atggtcgcga gcccgatgcc gagcgacagg ctgcagaagg
     2041 cggaaatggt cgcaacatcg atcgcgctca cgcccgccgt cgcgtagagg cgaaagcgga
     2101 tggcggcgcc ggtgaaggcg gcgaagccga gcgtgtgccc gaaggagtag gcgatgaagg
     2161 aggtgaaaag gatgcgcggg tagggcatcg cgcggcgcag gtaggcgagc gccagcacgt
     2221 cgtagttgga gagcagccag tagctcgccg cggtgaagcc gagcgccgcc agcacctggc
     2281 gtcgcgggat cgagtgcagg tgctcgagca cgctgcgcac gtgcaggtgc gcgagctggc
     2341 ggtgcaggat cagcaccacg gcggcgaaca ccagcagggc tgcgaccggc cccagccagc
     2401 gccggc
//
LOCUS       DATLKL010000305         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_150182, whole genome shotgun sequence.
ACCESSION   DATLKL010000305 DATLKL010000000
VERSION     DATLKL010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150182"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1048)
                     /locus_tag="VM713_03980"
     CDS             complement(188..1048)
                     /locus_tag="VM713_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVH83459.1"
                     /translation="MAAEYWFDSDDGLRLFARVYPAPGAAAPSVVCLHGLMRTGRDFE
                     DLAAHLAARYRVIVPDMRGRGLSARDPNFNNYQIPVYLADLTRALAGLAAARVSIIGT
                     SMGGLMAMVLAAMQPEVVAGIVLNDVGPEVDPAGLERIRGYAGKAPPVRDWEGAVKVV
                     RMVYGPALPGLDDARFEKLTRASYRADAQGVPQPDADPLIAEPLKDTSRAAPVLWPLW
                     GAIARVPMLAIRGAQSDLLSAGTLARMQREKPDLRTLTVADRGHAPLLDEPECMAAID
                     DFLTALGARA"
     gene            1093..1953
                     /gene="rlmJ"
                     /locus_tag="VM713_03985"
     CDS             1093..1953
                     /gene="rlmJ"
                     /locus_tag="VM713_03985"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HVH83460.1"
                     /translation="MKYRHSFHAGNFADVHKHVTLLALLAALKRKEKGFLYFDTHAGR
                     GGYDISGGGAEAAAGVGRFLASAPRAAELRHYAALLERLRAVRGSDHFYPGSPLLAVG
                     ELRPQDRAVLVEWQGAEAQALRTAVDAAAAPGATRTARPRARIEHGDGLKLLRAALPP
                     PERRALIFVDPPFEERRTEFAAVTAAVGDGLRRSATGVFALWYPIKEQRVVAAWQAEF
                     ARTVAAPTLAAELWLYPRDSRVALNGSGLLIVNPPWRIEERMREWLPELAAALGAGAA
                     GGTQVRMLSE"
BASE COUNT          305 a          759 c          728 g          289 t
ORIGIN      
        1 agcgccggcc gtggagaccg cgacccacgt caaggccgcc cgcacccgcc cgcggccggc
       61 ggcgcgcggc gtgcgcaagc cccacgccgc agccaagggc gcggccgcca agagcgcggc
      121 ccgcaagcgt ccgcagccca aggcggcgcc ggcgcgcgcg cgcggtgtca agtagcgcga
      181 gctagcctca agcgcgcgcg ccgagcgccg tcagaaagtc gtcgatcgcc gccatgcact
      241 ccggctcatc gagcagcggc gcgtggccgc gatccgcgac cgtcagcgtg cgcaggtccg
      301 gcttctcgcg ctgcatccgc gcgagcgttc cggcgctcag gaggtcggat tgcgcgccgc
      361 ggattgccag catcggcact cgcgcaatcg cgccccacag tggccagaga accggggccg
      421 ccctggaggt gtctttcagc ggctcggcaa tcagcggatc ggcgtcgggc tgcggcacgc
      481 cctgcgcatc ggcccggtag ctcgcgcgcg tcagcttctc gaagcgcgcg tcatcgaggc
      541 cgggcaatgc cggcccgtac accatgcgca ccactttcac ggccccctcc cagtcccgca
      601 cgggaggagc cttgccggca tagccccgga tgcgctcgag tccggccgga tccacctccg
      661 gccctacatc gttcagcacg atgcccgcca ccacctcggg ctgcatcgcc gccagcacca
      721 tcgccatgag gccgcccatt gaggtgccga tgatgctgac acgcgcagcg gcgaggcccg
      781 cgagcgcccg cgtgaggtcg gccagataca ccgggatctg gtagttgttg aaattcggat
      841 cgcgcgccga gagcccgcgg ccgcgcatgt cgggcacgat cacgcggtag cgcgccgcga
      901 ggtgcgccgc aagatcctca aagtctcgcc cggtgcgcat cagtccatgc aggcacacca
      961 ccgacggtgc cgccgcgccg ggcgcggggt agacgcgggc gaagagcctg agtccatcat
     1021 cgctgtcgaa ccagtattcg gccgccatct cgaacgctcc cgttggatga gcttttatac
     1081 tgcaaacgcg ctatgaaata ccgccacagt ttccacgcag gcaacttcgc cgacgtgcac
     1141 aagcacgtga cgctgctcgc gctgctcgcg gcgctcaagc gcaaggagaa gggcttcctc
     1201 tacttcgata cgcacgccgg ccgcggcggt tacgacattt cgggcggtgg cgctgaggcc
     1261 gccgccgggg tcgggcgctt cctcgcgagc gcgccgcgcg ccgcggagct gcgccactac
     1321 gccgccctcc tcgagaggct gcgcgcggtg cggggctccg accacttcta cccgggctca
     1381 cccctcctgg cggtcggcga gctgcgtccg caggaccgcg cggtgctcgt cgaatggcag
     1441 ggcgcggagg cgcaggcgct gcggaccgcg gtcgacgcag ccgcggcacc cggcgcgacg
     1501 cggacggcgc ggccgcgcgc gcgtattgag cacggcgacg gactgaagct gctgcgcgca
     1561 gcgctcccgc cgcccgagcg gcgcgcgctg atcttcgtcg atccgccgtt cgaggagagg
     1621 cgcacggaat ttgccgccgt gaccgccgcg gtcggcgacg ggctgcggcg ctccgcgacc
     1681 ggcgtgttcg ccctctggta cccgatcaag gagcagcgcg tcgtcgcggc ctggcaggcg
     1741 gagttcgcgc gcacggtcgc ggcgccgact ctcgccgcgg agctgtggct gtatccgcgc
     1801 gactcgcgcg tggcgctcaa cggctcgggg ctgctgatcg tgaatccgcc ctggcggatc
     1861 gaagagcgca tgcgcgagtg gctgccggag ctcgccgcgg ccttgggcgc cggggctgcg
     1921 ggaggcacgc aggtacggat gctgtcagaa taggttaaat gaacgctgca gccgatccct
     1981 acggagtcgt cgggcatccg atcagtcaca gcctctcgcc gttcattcac ggtctgtttg
     2041 cacgcgagac cgcgcaggcg atgagctatc gcctctacga t
//
LOCUS       DATLKL010000306         1504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_104932, whole genome shotgun sequence.
ACCESSION   DATLKL010000306 DATLKL010000000
VERSION     DATLKL010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104932"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VM713_03990"
     CDS             complement(<1..916)
                     /locus_tag="VM713_03990"
                     /inference="COORDINATES: protein motif:HMM:NF008872.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HVH83461.1"
                     /translation="MALKIMILGVNGFIGSALTDAILRTRDWEVYGMDLTDNKLGDLP
                     QNDRFHFVEGDITINREWVEYHVKKCDVIVPLVAIANPAQYVTHPLRVFELDFEANLA
                     VVRQCAKYGKRLVFPSTSEVYGMSPDAVLDEESSPLVYGPINKQRWIYAASKQMLDRV
                     IYAYGVRDNLDYTLFRPFNFIGPNLDNVEEPKEGSSRVFTQFLSNVLYKRPIKLVDGG
                     SQKRSFTYIDDAIECLLIILENHNRRASQRIFNIGNPANCVSIRDLAQRTIRIAQEFP
                     ILRDNARATEIVDVSAGEYYGKYYQDIQV"
     gene            complement(1018..>1504)
                     /locus_tag="VM713_03995"
     CDS             complement(1018..>1504)
                     /locus_tag="VM713_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83462.1"
                     /translation="PRDSGTALAGLRQKFALAMEVYSDQDALHRAVNEIRAARTRIAA
                     LSAGAKGKPRGASLEADGTALLAQAAKIEGLLMQVNIKGSEANLNYPGMLNEQIYSFS
                     LLLEDADTAPNEQETETYTGMHARLAAQLADWSALKIRQVAAFCERARAAGIDAAASC
                     P"
BASE COUNT          251 a          447 c          507 g          299 t
ORIGIN      
        1 gcacctggat gtcctggtag tacttgccgt agtactcgcc cgccgacacg tcgacgatct
       61 cggtcgcgcg ggcgttgtcc cgcaggatcg ggaattcctg ggcgatgcgg atggtgcgct
      121 gcgcgagatc gcggatcgag acgcagttgg cgggattgcc gatgttgaag atgcgctggc
      181 tggcgcgccg gttgtggttc tcgaggatga tcagcagaca ctcgatggca tcgtcgatgt
      241 aagtgaagga gcgcttttgg ctgccaccgt cgaccagctt gatgggccgc ttgtagagca
      301 cgttggacag gaactgggtg aagacccgcg agctgccctc cttcggctcc tcgacgttgt
      361 cgaggttcgg tccgatgaag ttgaacggcc ggaacagcgt gtagtcgagg ttgtcgcgca
      421 cgccataggc gtagatgacg cgatcgagca tctgcttgga ggcggcgtag atccagcgct
      481 gtttgttgat cgggccgtag accagcgggc tcgactcctc atcgagcacc gcgtccggcg
      541 acatgccgta gacctcggag gtcgaaggga acaccaggcg cttgccgtac ttggcgcact
      601 ggcgcaccac ggcgagattg gcctcgaagt ccagctcgaa gacccgcagc ggatgggtga
      661 cgtactgcgc gggattcgcg atcgccacca gtggcacgat cacgtcacac ttcttgacgt
      721 gatactccac ccactccctg ttgatggtga tgtcgccttc cacgaaatga aaacggtcgt
      781 tctgcggcag atccccgagc ttgttgtcgg tgaggtccat gccgtagacc tcccagtcgc
      841 gcgtgcggag gatagcgtcg gtaagggcgc tgccgatgaa accgttgacg ccgagaatca
      901 tgatcttgag tgccataagg atcctcgccc gcggtgtcta tgcgcgggca gcaaagccga
      961 gaagtttagc atttttgcgg gcttagagcc gtctgcagtc cctgttgcgg cgcagcgcta
     1021 cgggcaggac gcggcggcgt cgattcccgc cgcgcgcgcc cgctcacaga atgccgcgac
     1081 ctgcctgatc ttcagggcgc tccagtcagc gagctgcgcg gcgagccgcg cgtgcatgcc
     1141 ggtataggtc tcggtctcct gctcattggg tgccgtgtcg gcgtcctcga gcagcaggct
     1201 gaaggaatag atctgctcgt tcaacatgcc cggatagttg agattggctt ccgagccctt
     1261 gatgttcacc tgcatcagca ggccttcgat cttcgcagcc tgcgccagca gcgccgtgcc
     1321 gtcagcttcg agcgacgcgc cgcggggctt gccctttgcg ccggcggaca acgccgcgat
     1381 gcgggtccgg gcggcgcgga tctcgttgac ggcgcgatgc agtgcgtcct gatcgctgta
     1441 gacctccatg gcgagagcga atttctgccg gagcccggcg agcgcggttc ccgagtcgcg
     1501 cgga
//
LOCUS       DATLKL010000307         1680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_82302, whole genome shotgun sequence.
ACCESSION   DATLKL010000307 DATLKL010000000
VERSION     DATLKL010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82302"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VM713_04000"
     CDS             complement(<1..216)
                     /locus_tag="VM713_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HVH83463.1"
                     /translation="MRRARAASLIGAPTDVGAADRGASMGPEALRVAGLAAALGQREL
                     DVVDLGNLSGPGNPWQPPQHGYRHLPQV"
     gene            complement(213..1304)
                     /locus_tag="VM713_04005"
     CDS             complement(213..1304)
                     /locus_tag="VM713_04005"
                     /inference="COORDINATES: protein motif:HMM:NF005762.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HVH83464.1"
                     /translation="MPERRAVMVDFIGIERVQELVSRRGAARFIEELAREIESDFLRW
                     SEFEKSPRHATHSAVGVIELMPASDGRLYAFKYVNGHPKNTALGLLTVTAFGVLADVQ
                     TGYPLLLSELTLTTALRTAATSALAARYMARPGSRVMALIGNGAQSEFQAIAFHHLLG
                     IRELRLYDTDGLATEKLVRNLGALRLPGLEVLRFGSTAAAVAGADIVTTVTADKRNAT
                     ILTPAMIAPGMHLNAVGGDCPGKTELHADILRRSDARVVVEFEPQSRIEGEIQQLPAD
                     FPVTELAEVLAGRASGRASEREVTVFDSVGFALEDFSSLRYLMRIHQEERGSERQIDL
                     IPDLEDPKDLFGLLAERSAQPAARSGVAA"
     gene            1411..>1680
                     /locus_tag="VM713_04010"
     CDS             1411..>1680
                     /locus_tag="VM713_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVH83465.1"
                     /translation="MDDIDRQLIALLRNDARASVASLAKALGVARGTVQNRMARLTAN
                     GTIVGYTVRLKPDVEEQRMRAFMTVAVEGTQVDAVIRALRGEPAVG"
BASE COUNT          269 a          581 c          558 g          272 t
ORIGIN      
        1 gacctgcggc aggtgccggt agccgtgctg cggtggctgc caggggttcc cggggccgct
       61 gagatttcca agatcgacta cgtccagctc gcgctgcccg agagccgccg cgagcccggc
      121 gacgcgcagc gcctcgggcc ccatcgacgc cccgcgatcc gccgcgccca catcggtggg
      181 cgcgccgatc aggctcgccg cacgcgcgcg cctcatgccg ccacccccga gcgggccgcg
      241 ggctgcgcgc ttctctcggc cagcaacccg aacaggtcct tgggatcctc gagatcgggg
      301 atcaggtcga tctgccgctc actgccgcgc tcctcctggt gaatgcgcat cagatagcgg
      361 agcgaggaga aatcctcgag cgcaaagccg accgagtcga agacggtcac ttcgcgctcg
      421 ctcgcacggc cgctcgcgcg tcccgcgagc acttcggcca gctcggtgac cgggaaatcg
      481 gccggtagct gctggatctc gccttcgatg cgcgactgcg gctcgaactc caccactacc
      541 cgggcgtcgg accgcctgag gatatcggca tgcagctcgg tctttcccgg gcagtcgcca
      601 cccacggcgt tcaggtgcat cccgggggcg atcatcgccg gtgtcaggat ggtggcgttg
      661 cgcttgtccg cggtcaccgt ggtgacgatg tccgctccgg ctaccgcggc cgccgtcgag
      721 ccgaagcgca gcacctcgag gccgggcaga cgcagcgcac ctaagttgcg caccagcttt
      781 tcggtggcga gcccatcggt gtcgtagagg cgcagctcgc ggatgccgag caggtggtga
      841 aacgcgatcg cctggaactc gctctgcgca ccgttaccga tcagcgccat gacgcgactg
      901 ccgggccggg ccatgtagcg cgccgcgagc gccgaagtcg ccgcggtgcg cagtgccgtc
      961 gtgagggtga gctccgacag cagcagcgga tagcccgtct gcacgtcggc gagcacgccg
     1021 aacgcagtca cggtgagcag tccgagcgcc gtgttcttcg gatggccgtt cacgtacttg
     1081 aaggcataga gccgcccgtc ggaggcgggc atgagctcga tcaccccgac ggccgagtgg
     1141 gtcgcgtggc ggggcgactt ctcgaattcg ctccagcgca ggaagtcgct ctcgatctcg
     1201 cgcgcgagct cctcgatgaa acgcgccgcg ccccggcggg ataccaactc ctggacgcgc
     1261 tcgatgccga tgaaatcgac catgactgct ctcctttcag gcatggtcgc gccctcctgg
     1321 accaaagaaa agacagaata ctgctcaatc attgtagcta tttgatcatt ctgtcatgac
     1381 attctggcgt tttttggtat cctttgggcc atggatgaca tcgaccgcca gctcatcgcg
     1441 ctactgcgca atgacgcccg cgcctcggtg gcttcgctcg ccaaagcgct cggcgtcgcc
     1501 cgggggacgg tgcagaaccg gatggcacgc ctcaccgcca acggcacgat cgtcggctac
     1561 acggtgcgcc tgaaacccga cgtcgaggag cagcgcatgc gcgccttcat gaccgtcgcg
     1621 gtcgagggaa cccaggtcga cgccgtgatc cgggcgctcc ggggcgagcc ggccgtgggc
//
LOCUS       DATLKL010000308         2439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_48451, whole genome shotgun sequence.
ACCESSION   DATLKL010000308 DATLKL010000000
VERSION     DATLKL010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48451"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1248
                     /locus_tag="VM713_04015"
                     /pseudo
     CDS             76..1248
                     /locus_tag="VM713_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1439..2128
                     /locus_tag="VM713_04020"
     CDS             1439..2128
                     /locus_tag="VM713_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HVH83466.1"
                     /translation="MAFPDTPRALRAVRSPLIDAGFIVGYAAVRGGGEYGREWHKAGQ
                     LMNKPNTWHDLIDVCEELCKLKYTAPERLAIGGRSAGGITVGRALTERPELFAAVVDG
                     VGWSNPLRYVAEQNGYGEEPEWGAIRDESGYRALKLIDSYQAVQDGVAYPAVLLTTGV
                     TDPRVAPFHVAKMAARLQVASSSGKPILLRVDFDAGHGIGSTRSQQDREAADTYAFLL
                     WRTGVKDYQPA"
     gene            2219..>2439
                     /locus_tag="VM713_04025"
     CDS             2219..>2439
                     /locus_tag="VM713_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83467.1"
                     /translation="MCNFIARVVASISVLAFLGVAGCGGSGGSGSMMSLSVGDAPVDG
                     AEKVVVVFSGVQLIPDAGDPVTINFAAPRT"
BASE COUNT          381 a          845 c          836 g          377 t
ORIGIN      
        1 caagccgccg gagccggggc cgcggcggtc gccgcctcgg ccggatcggc catcccgccc
       61 gcgccggtgg cgcgcgtggc ggtggtcacc gacagctatt tcggcgagac ggtgcgcgat
      121 ccgtatcgct ggatggagaa cgaccaggat tcggagtggc tgccctatct caaggggcag
      181 aacgcgcaca cgcgcgcggt gctcgagcgg attccgcacc gggagaagct gctcgcgcgc
      241 atccagcagc tttcgggaga tgtgacgctc acgcggcgcg tgcagcgcgc cggcggcagg
      301 ctcttcatcg agcagcggcc ggcgggtgcc gacaactatc gcctgttcgt cgaggagcat
      361 ggcgtgcgcc gcctgctcat cgaccccacc gcgctctcga gcgccaccag ccacatgtcg
      421 ctcgactggt ggcacgcctc gcccgacggg acgcacctcg tctacgggct gtcgaaggac
      481 ggcagcgagg actcggtcct gcacatcttg aggaccgccg atggcaccaa cctcccggag
      541 cgcatcgcca acacccaagc cgccaacccg gcgtggctcg atgacggcag cggcttcttc
      601 tacaaccagc tcaccggcaa ggtcgacacg cccgagcgct atctcgatag ccaggcgcgc
      661 ttccaccggc tcggtgccga ccccgagacc gatcccatcc tcatgaagcg tgggctcgat
      721 cctgacgtgc agtacgagaa aatccaggcg ccggccatcg aaacccacta cggctcgccc
      781 tacgcgctgc tcacgctctc ggacgtacgt agcgagtcgc gcctgcttat agcgaaggtc
      841 gcagacgctg cggccaaccg cgcccgctgg gtgccggtcg ccgccttcga ggacgaagtg
      901 accgacgccg tgatcgacgg cgagacgctc tatctgctcg ccaacgaggg tcacccgcgc
      961 ggccgcattc tccaggcctc cgtgagtgcg cccgccgtga gggacgcccg cgagctggtc
     1021 gggcagggca gcgccgtcat cgaagggctc gcgcgggcgc gcgacggcgt gtacctgagg
     1081 atgatggacg ggggcatcgc cggattgaag cgcctcggac gcgacggccg agtggccgac
     1141 atcgcgctgc ccttcgacgg cacgctgacg gcgctctcga ccaactcgac cgaggagggc
     1201 gcgctgttct ccttcaccgg ctggctcaac ccggcggaca tctggtcggt ggacgcacag
     1261 ggcaaggtgg ccgcgactgg cttgactccg cgcccgccga tcgagacttc acgctacgag
     1321 accgagcggc gcttcgcgac cgcgcgcgac ggcacgcgca ttccctactc gctgatcttc
     1381 aggaaggggc ttaagcgcga cggcgcgacc cccgcctgga tctccgcgta cggctcgtat
     1441 ggctttcccg gatacacccc gagctttgcg ggccgtacgc tcgcccctca tcgacgccgg
     1501 tttcatcgtc ggctacgccg ccgtgcgcgg cggcggcgag tacggccgcg aatggcacaa
     1561 ggccgggcag ctgatgaaca agcccaacac ctggcacgac ctcatcgacg tctgcgagga
     1621 gctctgcaag ctcaagtaca ccgcgcccga gcgtctcgcc atcggtggcc gctccgccgg
     1681 cggcatcacc gtgggacgcg cgctcaccga gcgcccggag ctgtttgccg cggtagtcga
     1741 cggggtcggt tggtcgaatc cgctgcgcta cgtcgccgag cagaacggct acggcgaaga
     1801 gcctgagtgg ggcgcgatcc gcgacgagtc gggctatcgc gcgctcaagc tcatcgacag
     1861 ctaccaggcg gtacaggacg gcgtcgcgta tccggcggtg ctgctcacga ccggtgtcac
     1921 cgatccgcgc gtcgcgccgt ttcacgtcgc gaagatggcg gcgaggcttc aggttgcgtc
     1981 gagctccggg aagccgatcc tgctgcgcgt cgatttcgac gccggccacg gtatcggctc
     2041 gacgcgctcg cagcaggacc gcgaggcggc cgacacgtac gcattcctcc tgtggcgcac
     2101 cggcgtgaag gactaccagc cggcgtgacc gtgagcggcg cgtgtgttca gccttcgttc
     2161 agcgcgccgc tgccggaatg catggcttcc gggacgtggc cctggggaag gaggccgcat
     2221 gtgcaatttc atcgcgaggg ttgtggccag catctcggtg ctcgccttcc tcggtgtggc
     2281 gggctgtgga ggtagcggcg gttccggcag catgatgagt ctgtcggtcg gcgatgcgcc
     2341 ggtcgatggg gcggagaaag tggtggtcgt gttctccggc gtgcaactca tcccggacgc
     2401 cggcgatccc gtcaccatca actttgcggc cccgaggac
//
LOCUS       DATLKL010000309         2157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_82332, whole genome shotgun sequence.
ACCESSION   DATLKL010000309 DATLKL010000000
VERSION     DATLKL010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82332"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1779
                     /locus_tag="VM713_04030"
     CDS             124..1779
                     /locus_tag="VM713_04030"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HVH83468.1"
                     /translation="MYRYDEFDQRLVDERVAQFRDQVRRRLDGELSEDEFKPLRLMNG
                     LYLQLHSYMLRIAIPYGALSARQMRKLAHIARRYDKGYGHFTTRQNIQFHWPKLAEVP
                     DILAELAEVQMHAIQASGNSFRNVTADQYAGVAADEIEDPRIYGEIIRQWSTLHPEFS
                     FLPRKFKIAISGAARDRAAVKTHDIGLFIRRGDGGEVGFEVVVGGGQGRPPMIAKTVR
                     AFLPKEDLLAYLEAIMRVYNEFGRRDNLYKARIKILVHELGIEKFTAMVEEEWAASRA
                     YATPLPPEEIERIRAHFAPPAYETIATPVASFEAKRFEDREFARWVRHNVAAHRQPGY
                     AIVNLSLKPVGETPGDVTAEQMDAIADLADRYSLGEIRVTHEQNLVLPHVKQADLHAL
                     WQSLAALSLATPNIGLATDIIACPGLDYCSLANARSIPVAQRVARRLADFDRQEQIGE
                     LRINISGCINACGHHHVGHIGILGVDKQGEEFYQVTLGGNAEEDASLGSILGPAFSSD
                     EIAGAVETIVDTYLAQRRDGERFVDTCRRVGLQPFKEKLYGAH"
     gene            1766..>2157
                     /locus_tag="VM713_04035"
     CDS             1766..>2157
                     /locus_tag="VM713_04035"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HVH83469.1"
                     /translation="MALIKEGQIAADPWRGLADDEALPAEGRIVISLERWQREQNALL
                     ARGAPLGIRLKAGQHPQAISGHVQLFALVALEFTKFTDGRPYSYARLLRQRYGYEGEL
                     RATGQVLRDQLLFMQRCGFDAFECANADG"
BASE COUNT          369 a          750 c          704 g          334 t
ORIGIN      
        1 ctcatcgacg tgttggagga agcgggctcg atccggccgg cgcgacggcg tgagcgcatt
       61 cgcggcgcgg gtcccacggt caggcccgat ctcggctacc aggcggggga ggaggcgtga
      121 gccatgtacc gctacgacga gttcgaccag cgcctcgttg acgagagggt cgcccagttc
      181 cgcgaccaag tgcgccggcg cctcgacggg gagctttcgg aggacgagtt caagccgctc
      241 cggctgatga acgggctcta tctgcagctc cattcctaca tgctgcggat cgccatcccc
      301 tacggcgcgc tctccgcgcg gcagatgcgc aagctggcgc acatcgcgcg gcgttacgac
      361 aaggggtacg gccacttcac cacgcgccag aacatccagt tccactggcc gaagctcgcg
      421 gaggtgcccg acatcctggc cgagctcgcg gaagtgcaaa tgcacgcgat ccaggcgagc
      481 ggaaactcgt tccgcaacgt cacggccgac caatatgccg gcgtcgccgc cgacgagatc
      541 gaggatccgc gcatctacgg cgagatcatc cgccagtggt cgacgctgca cccggagttc
      601 tccttcctgc cgcgcaagtt caagatcgcg atctcggggg ccgcgcgcga ccgcgctgcc
      661 gtgaagactc atgacatcgg cctcttcatc cggaggggag acgggggcga ggtcggcttc
      721 gaggtggtgg tcgggggcgg gcagggccgc ccgcccatga tcgccaagac cgtgcgcgcg
      781 ttcctgccca aggaggacct gctcgcctat ctcgaggcga tcatgcgcgt ctacaacgag
      841 ttcgggcggc gcgacaacct ctacaaggcc cgcatcaaga tcctcgtcca cgagctcggg
      901 atcgagaaat tcaccgccat ggtcgaggag gaatgggccg cgagcagggc ctacgccacg
      961 ccactcccgc ccgaggagat cgagcgcatc cgcgcccatt tcgccccccc ggcctacgag
     1021 accatcgcga ctccagttgc gagcttcgag gcgaagcgct tcgaggaccg cgaattcgcg
     1081 cgctgggtgc gccacaacgt cgcggcgcat cggcagccgg gctacgcgat cgtcaacctc
     1141 tcgctcaagc cggttggcga gactccgggc gacgtgacag cagagcagat ggatgcgatc
     1201 gccgatctcg cggatcgcta cagcctgggc gagatccgcg tcacccacga gcagaacctg
     1261 gtgctgcctc acgtgaagca ggcggacctt cacgcactct ggcagtcgct cgcggccctc
     1321 tcgctcgcca cgcccaacat cgggctcgcc accgacatca tcgcctgccc gggcctcgat
     1381 tattgcagcc tcgccaatgc gcggtccatt cccgtggctc agcgcgttgc ccgccgcctt
     1441 gccgatttcg accgacagga gcagatcggc gagctccgca tcaacatctc gggctgcatc
     1501 aacgcctgcg gccatcacca tgtggggcac atcggcatcc tcggcgtcga caagcagggc
     1561 gaggagttct accaggtcac gctcggcggc aatgccgagg aggacgcctc gctcggctct
     1621 atcctcggcc cggccttctc gagcgacgag attgcgggcg cggtcgagac catcgtcgac
     1681 acctatcttg cgcagcggcg ggacggcgag cgcttcgtcg acacctgccg ccgcgtcggg
     1741 ctccagccgt tcaaggagaa gctctatggc gctcattaag gagggacaga tcgccgcgga
     1801 cccctggcgc gggctcgcgg acgacgaggc gctccccgcc gagggccgca tcgtaatcag
     1861 cctcgagcgg tggcagcgcg agcagaacgc gctcctcgcg cgcggggcgc cgctcgggat
     1921 ccggctcaag gccggtcagc acccgcaggc gatctcaggg catgtgcagc tcttcgcgct
     1981 ggtcgcgctc gagttcacca aattcacgga cggacgcccc tactcctacg cgcgcctcct
     2041 gcgccagcgc tatggctatg agggcgagct gcgcgcgacg ggccaagtgc tgcgcgatca
     2101 gcttctcttc atgcagcgtt gcgggttcga tgcattcgaa tgcgctaacg ccgacgg
//
LOCUS       DATLKL010000310         6076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_138950, whole genome shotgun sequence.
ACCESSION   DATLKL010000310 DATLKL010000000
VERSION     DATLKL010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138950"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VM713_04040"
     CDS             <1..1386
                     /locus_tag="VM713_04040"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HVH83470.1"
                     /translation="RILFLGFGSIGQGVLPLILRHIGIAPERITIVTAEDKGHEEAAK
                     YGIKFLKERLTRENFRRVLNPLLGRGDFLINVSVEVSSVALIKLCWEKGAMYIDTCIE
                     PWPGGYTDPTVPVARRTNYALREEALALRAGSERAPTAVLTHGANPGLVSHFVKQALL
                     NIAQDTQTEAGRPATRAEWGELARRLGVKVIHVAERDTQISSTPKLPGEFVNTWSIDG
                     FVSEGSQPSEMGWGTHERNFPRDGKRHDFGCLAAIYLMQPGAGTRVRTWTPRAGHFHG
                     FCITHGESISIADYYTVRDGAQVVYRPTVHYAYHPCDSAVMSVHELAGRNYVQQERQR
                     ILMDDITAGIDELGVLLAGHRRNAYWYGSQLSIGEARKLVPYNNATSLQVTVAVLSGV
                     VWAMENPNQGLVEPDELDFRRNLEICMPYLGPVSGEYTDWTPLHERERLFPEDVDKSD
                     PWQFKNVRVLW"
     gene            complement(1394..1894)
                     /gene="greB"
                     /locus_tag="VM713_04045"
     CDS             complement(1394..1894)
                     /gene="greB"
                     /locus_tag="VM713_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HVH83471.1"
                     /translation="MGRAPPREAEGSKYITPQGAERLRSELDELWRGERPRVTQAVAA
                     AAAQGDRSENAEYTYGKRRLREIDRRVRFLRRRLDGMVVVDQPPADFSRVFFGAWVRV
                     EAEDGAQSRYRIVGPDEFDMAPGYISMDSPLGKALLRRRLDEEVVVETPAGARAYVIV
                     AIEYRP"
     gene            2000..2203
                     /locus_tag="VM713_04050"
     CDS             2000..2203
                     /locus_tag="VM713_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83472.1"
                     /translation="MATKYYTHFVTGIGKAHSAPGNEWSGVVELREPLTQARANRELR
                     SLLAQSFDLDTDEIRILHWARLH"
     gene            2369..3019
                     /locus_tag="VM713_04055"
     CDS             2369..3019
                     /locus_tag="VM713_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HVH83473.1"
                     /translation="MRAWMVALLSAAVLASSGCGYNTLQQQDESVKAAWSEVVNQYQR
                     RADLIPNLVEVVKGYAAQEQKVLIGVTEARARATSIQVTPQVLNNPELFQKYQAAQGE
                     LTQALSHLLAVTENYPQLKSDQRFADLQSQLEGTENRITVARNRYIQAVQQYNVTVRS
                     FPVNLTAKMFDFQVKPNFTVANEQQIATAPTVSFDTAAPAASAPSTPAATGQPAKQ"
     gene            3024..3926
                     /locus_tag="VM713_04060"
     CDS             3024..3926
                     /locus_tag="VM713_04060"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HVH83474.1"
                     /translation="MSRNALRAFHWVGVALPLVFGFALVAGAQDLQPLPKLSARVTDL
                     TGTLTAAQQTALEQKLSAFESAKGTQLAVLIVPTTQPEALEQYSIRVVDAWKLGRKGV
                     DDGALLIVAKDDHKVRIEVGRGLEGVLTDALCNRILDETVKPQFRQGDFYGGIDAGLG
                     QMMKLIQGEPLPPPERQWQPGRRAHGGGLLLPLLFAVFVGSVVLRGIFGRTVGSVLTG
                     AGAGLLVWLAGYALAFAGLAAMGAFVFTLVGGLGRGSGWSSGGYGGGWGGFGGGLGGS
                     GGFGGGGFSGGGGGFAGGGASGSW"
     gene            3930..4427
                     /locus_tag="VM713_04065"
     CDS             3930..4427
                     /locus_tag="VM713_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HVH83475.1"
                     /translation="MKLTRLLRHLLTTRFSTRRHFPPAVRAAIEHAIKECEARHAGEL
                     RFVVETAYDLPELWHDLPPRQRALQLFGQLGVWDTAHNNGVLIYVDMADRVVEIIADR
                     GIAARVPQAEWAAVCRQMEHHYRGGRFREGSVVGILGVGALLGRHFPGRTSGGHELPE
                     QPVLL"
     gene            4478..5725
                     /locus_tag="VM713_04070"
     CDS             4478..5725
                     /locus_tag="VM713_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HVH83476.1"
                     /translation="MKIVRVRAALGALACGSMLAACSLFGPRHHEAPPPAPPPAPAAP
                     ALPLPVATERFELESGQSVVGGVQVVTAAKDDTLTDIARRFNVGYEELLRANPKMDPW
                     LPGEGHEIVVPTQFVLPNAPYKGVVINIAAMRIYYFPPAKRGEPVVVLTHPIGIGKVG
                     WRTPEGVTKIVRRQQDPTWRVPVSVLKEHHDNGEDLDPVIGPGPDNPLGKYAFYLQWP
                     SYLIHGTNKPAGVGLRSSHGCIRLYPEDIEQFFNMIPVGTEVRVVNQPFLFGWGNGQL
                     YLQPFDVLEDDERDWSKAQRKLLSKSLPAALQQQLKDHHQQIDWDLVASLAHNPRGIP
                     VPVSTTDASLEQVLAAAPRVQNVLPEGSTWDGTSDLPMDEASFRQMFSEIEPGASAPA
                     ASAPTPTGAAVGKTPPPVPKDGS"
     gene            5725..5901
                     /locus_tag="VM713_04075"
     CDS             5725..5901
                     /locus_tag="VM713_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83477.1"
                     /translation="MAGEPAPDALSPSQSRGPALSQWLQLMVAEIAAKREALERARAE
                     EARRHAERADSHQT"
BASE COUNT         1018 a         2055 c         2042 g          961 t
ORIGIN      
        1 aggatcctct tcctcggctt cggcagcatc ggccagggcg tgctgccgct catcctgcgg
       61 cacatcggca ttgcgcccga gcggatcacc atcgtgaccg ccgaggacaa gggccacgag
      121 gaggccgcca agtacggcat caagttcctc aaggagcgcc tgacgcgcga gaacttccgc
      181 cgcgtgctca atccgctgct cgggcgcggc gacttcctca tcaacgtgtc ggtggaggta
      241 tcgagcgtcg cgctcatcaa gctgtgctgg gaaaagggcg cgatgtacat cgatacctgc
      301 atcgagccct ggccgggcgg ctacaccgat ccgacggtgc cggtcgcccg gcgtaccaac
      361 tatgcgctgc gcgaggaggc gctggcgctg cgggcgggca gcgagcgtgc gccgaccgca
      421 gtgctcaccc acggtgccaa tccggggctg gtgtcgcact tcgtcaagca ggcgctgctc
      481 aacatcgccc aggacacgca gaccgaggcc ggcaggcccg cgacgcgcgc ggaatggggg
      541 gagctggcgc gccggctcgg ggtcaaggtg atccacgtcg ccgagcgtga cacccagatc
      601 tcgagcacgc ccaagctccc cggggagttc gtcaacacct ggtcgatcga cggtttcgtg
      661 agcgaaggat cgcagccgag cgagatgggc tgggggacgc acgagcgtaa ctttccgcgg
      721 gacggcaagc gtcatgactt cggctgcctc gcggcgatct atctgatgca gcccggggcc
      781 ggcacgcgcg tgcgcacctg gacgccgcgc gccggccact tccatggttt ctgcatcacc
      841 cacggcgaat cgatctcgat cgccgactac tacacggtgc gcgacggcgc ccaggtggtg
      901 taccgcccga ccgtgcacta cgcctaccac ccatgcgatt cggcggtgat gtcggtgcac
      961 gagctcgctg gccgcaacta tgtgcaacag gagcgccagc gcatcctcat ggacgacatc
     1021 acggcgggaa tcgacgagct cggcgtgctg ctcgccggac accggcgcaa cgcctactgg
     1081 tacggctcgc agctctcgat cggcgaggcc cgcaagctcg tgccctacaa caatgcgacg
     1141 agtctgcagg tgaccgtggc ggtactgtcg ggggtcgtct gggcgatgga gaatccgaac
     1201 caggggctgg tggaacccga tgagttggat ttccggcgca atctcgagat ctgcatgccg
     1261 tacttgggcc cggtgtcggg ggaatacacc gactggactc cgctgcacga gcgcgagcga
     1321 ctgtttcccg aggacgtcga caagagcgac ccctggcagt tcaagaacgt gcgggtactc
     1381 tggtagatcg gcttcagggc cggtactcga tcgccacgat cacgtaggcg cgcgcgccgg
     1441 ccggggtctc gaccacgact tcctcgtcaa gacgccgccg cagcaacgcc ttccccaagg
     1501 gcgagtccat gctgatgtac ccgggcgcca tgtcaaattc atcgggcccc acgatccggt
     1561 agcgcgactg cgcgccgtcc tcggcctcga cccgcaccca ggcgccgaag aacacccttg
     1621 agaagtcagc cgggggctgg tccaccacca ccatgccatc gagccggcgg cgcaggaagc
     1681 gcacacgccg gtcgatctcg cgcaggcggc gcttgccgta ggtgtattcg gcgttctcgg
     1741 agcgatcgcc ctgggccgcg gcggcggcga ccgcctgggt gacccgtggc cgctcgccgc
     1801 gccacagctc gtcgagctcc gaccgcagcc gctcggcccc ctggggggtt atgtacttgg
     1861 atccctcggc ctctcgcggg ggcgcgcgtc ccatggcccg aaagtcctgt gaattagttc
     1921 gcagcctttt tacagaagtc cggggttctg tggaacatct cacggacggg aaccgtagga
     1981 cgctactaac ctttgaaccg tggctaccaa gtactacacg cacttcgtaa ccggcattgg
     2041 caaggcgcac agcgccccgg gcaacgagtg gagcggcgtt gtggagcttc gggagccgct
     2101 cacgcaggcg cgcgccaacc gcgagctgcg ctcgctcctc gcgcagagtt tcgacctgga
     2161 cacggacgag atccgtatcc tgcactgggc ccgattgcac tgaatcccct ggcggacttc
     2221 cttcccttca tcgaaggtcc gcccttccag accccaggcc tgaaaacctg gggttttttt
     2281 tgcccttgat cgacgccgct gcggcccccg tgtgacaatc cgcacccccg gtcgccgggc
     2341 cggactcatc tctcgaggag aaactcccat gcgtgcgtgg atggtcgcgt tgctcagcgc
     2401 cgcggtgctg gcatcatcag gctgcgggta caacaccctg caacagcagg acgagagcgt
     2461 gaaggccgcc tggtccgagg tcgtcaacca gtaccagcgc agggctgacc tcatcccgaa
     2521 tctcgtggag gtcgtgaagg gctacgccgc ccaggagcag aaggtcctga tcggggtcac
     2581 cgaggcgcgc gcccgcgcta cctccattca ggtgacgccg caggtgctca acaacccgga
     2641 gctctttcag aagtatcagg ccgcgcaggg cgagctcacc caggccttga gccacctgct
     2701 ggcggtcacc gagaactacc cgcagctgaa atccgaccag aggttcgccg acctgcagtc
     2761 gcagctggag ggcaccgaaa accgcattac ggtcgcgcgt aaccgctaca tccaggcggt
     2821 gcagcagtac aacgtcacgg tgcgctcctt ccccgtgaat ctcaccgcca agatgttcga
     2881 ttttcaggtg aagccgaact tcacggtcgc caacgagcag cagatagcca cggcgccgac
     2941 ggtgagcttc gacactgcgg ccccggccgc gtcggcgccg agcacaccgg cggccacggg
     3001 ccagcccgcc aaacaatgac gcgatgagcc gcaacgcgct tcgggccttc cactgggtgg
     3061 gggtcgcgct gccgctcgtc ttcggcttcg ctctggtggc cggcgcacag gatctgcagc
     3121 cgctgccgaa gctcagcgcc cgtgtcacgg acctcaccgg gacacttacc gccgcgcagc
     3181 agaccgcact cgagcagaaa ctgtccgcgt tcgagtccgc caagggcacg caactcgcgg
     3241 tgctcatcgt gccgacgacg cagccggaag cgctcgagca gtactcgatc cgggtggttg
     3301 atgcctggaa gctcggccgc aagggggtcg atgatggcgc actgctcatc gtcgccaagg
     3361 acgaccacaa agtccgcatc gaagtggggc ggggactcga gggcgtgctc accgacgccc
     3421 tttgcaaccg catcctcgat gagaccgtga agccccagtt ccgccaggga gacttctacg
     3481 gcggcatcga cgcaggcctc ggacagatga tgaagctcat tcagggcgag cctctgccgc
     3541 cccccgagcg gcagtggcaa ccgggccggc gcgcccacgg cggcggactg ctgctgccgc
     3601 tgctgttcgc ggtgttcgtg ggctcagtgg tgctgcgtgg gatctttggg cgcaccgtcg
     3661 gctcggtgct gacgggggcg ggagcgggac ttctggtatg gctcgcgggc tatgccctcg
     3721 cgtttgcggg cctcgccgct atgggcgcat ttgtctttac gctggtcggc ggtctcgggc
     3781 gcggcagtgg ctggtcaagc ggcggctacg gcggaggctg gggcggcttc ggcggcggac
     3841 tgggcgggag cggcggtttc ggcggcgggg gtttcagcgg cgggggcggc ggcttcgccg
     3901 gcggcggcgc ttccgggagc tggtaagcca tgaagctcac gcgtctcctt cgccacctgc
     3961 tgaccacccg cttcagcaca cgccggcact ttccgccggc ggtgcgcgcc gcgatcgagc
     4021 acgccataaa ggaatgcgag gcgcgccacg cgggcgagct gcgcttcgtg gtggagacgg
     4081 cgtacgacct gccggagctc tggcacgatc tgccgccgcg tcagcgcgcg ctacagctgt
     4141 tcgggcagct tggggtgtgg gataccgcgc acaacaacgg cgtgctgatc tatgtggaca
     4201 tggccgatcg cgtggtcgag atcatcgccg accggggcat cgccgcgcgt gtgccgcagg
     4261 ccgagtgggc ggcggtgtgc cgccagatgg agcaccatta tcgtgggggg cggtttcgcg
     4321 agggttcggt cgtcggtatc ctcggcgtgg gcgcgctcct cggacgccat ttccccggca
     4381 ggacatccgg cgggcacgag ctgccggagc agccggtgct gctgtagtga atctgcgacg
     4441 gctggcccgc tacgacatcg gtcataatgc actacttatg aagattgttc gggtgcgcgc
     4501 ggcgctggga gcgctcgcgt gcggctccat gcttgctgct tgctcgctgt tcggtccgcg
     4561 gcaccacgaa gcaccaccgc ccgcgccacc gcccgcgccg gcggcaccgg cactaccgct
     4621 acccgtggcg accgagcgct tcgagctcga gagcggccag agcgtggtgg gcggcgtgca
     4681 ggtcgtcacc gccgccaagg acgataccct caccgacatc gcccgccgct tcaacgtcgg
     4741 ctacgaggag ctgctgcgcg ccaacccgaa gatggatccg tggctgccgg gcgaggggca
     4801 cgagatcgtc gtgccgacgc agttcgtcct ccccaacgcc ccctacaagg gcgtggtgat
     4861 caacatcgcc gccatgcgca tctactactt cccgcccgcg aaacgcggcg agcccgtcgt
     4921 ggtgctgacg catccgatcg gcatcggcaa ggtgggctgg cgtaccccgg agggggtcac
     4981 caagatcgtg cggcgccagc aagatcccac ctggcgcgtg ccagtgtcgg tgctcaagga
     5041 gcaccacgac aacggcgagg atctcgatcc ggtgatcggc ccgggtccgg acaaccccct
     5101 tggcaagtat gcgttttacc tgcagtggcc gagctacctc attcacggca ccaacaagcc
     5161 ggccggcgtc ggattgcgct cgagccacgg ctgcatccgc ctctacccgg aggacatcga
     5221 gcagttcttc aacatgatcc cggtcggaac ggaggtgcgg gtggtgaacc agccgttcct
     5281 gttcggctgg ggtaatgggc agctctatct gcagccgttc gatgtgctgg aggacgacga
     5341 gcgcgactgg agcaaggcgc agcgcaagct cctcagcaag tcgctccccg ccgcactgca
     5401 acagcagctc aaggaccacc accagcagat cgattgggat ctggtggcct cgctcgccca
     5461 caacccgcgc ggaatcccgg tgccggtctc gacaaccgat gcgagcctcg agcaggtgct
     5521 cgccgccgcc ccgcgggtac agaacgtgct tcccgagggt tccacctggg atggcacctc
     5581 cgacctgccg atggacgagg catccttcag gcagatgttc tcggagatcg agcccggcgc
     5641 gagcgcgccg gccgccagcg cgccgactcc cacaggcgcg gccgtgggca agactccgcc
     5701 gccggtaccc aaggacggca gctgatggcg ggcgagccgg cgccggacgc gctgtcaccg
     5761 tcgcagtcgc gcggcccggc actttcgcag tggctgcagc tgatggtggc ggagatcgcc
     5821 gccaagcgcg aggcgctcga gcgtgcccgt gccgaggaag cgcggcgcca cgcggagcgc
     5881 gccgactcac accagactta agtcgccaac gcgccgagat tgtgcctaca gcggtgatcc
     5941 gctgtgctaa agtcccgcgg gcacaatacc aagaggcgtt gagtggccat cagccgtagc
     6001 ggcagcgcac aactcgccca acaaagccgt gacctgcacg ccatcgacgg agtcgcgacc
     6061 ggaattgccg ccttcg
//
LOCUS       DATLKL010000311         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_37367, whole genome shotgun sequence.
ACCESSION   DATLKL010000311 DATLKL010000000
VERSION     DATLKL010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37367"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2057
                     /locus_tag="VM713_04080"
     CDS             <1..>2057
                     /locus_tag="VM713_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83478.1"
                     /translation="ILAPGVPDAHSPLVVRTGTGSIDLAAAGDLRLGNQASVIYTAGA
                     QGPPNFATARDGTSYLAQEGGSVTVRVGRDIVGATSDQLFTNWLFRSGLNSSNLRGGF
                     QPTAWGISYDNFEQGIGALGGGDLTISAGRNIIDLGADVPSIGVPVGTAHSATTVEEN
                     PGTLSIESGGEILGGKFLDMAGVASVVAGGAILTGSTQAIANGSPTGLNLILGTGSGQ
                     FSVTARSDLLFDAATAVTLIPRARTQAVTGGTTYFGTFTDASSLTLESVGGTLDFTNR
                     SRLIEESAPAQSPRFDSQASDDQPGLHILAPTFGAIAFGGDLRVSGSVALWPSAHGNL
                     DLLAAGSVQLNDAPVLVSDVDPGRLPSANAVSQSIVQNVDPILGLLDPRSPDGAATLS
                     TPFLHAPLPVHGGAYASDGQPDRVPIRIVALTGDVSLQPASNGTVSPIDVPKAIDIFA
                     GKDIVNLDLNVQQDTSADASSIVAGGNITYPFNRIFTGVLVPSNAAINVAGPGNFEIQ
                     AGGTINLGTSLGISSIGNIYNPALPATGANLSVAAGISAAPQYPAFISTYLADGSTYD
                     QQLEQYIADTEGVQNPTKAQALALFEALPTAQQVPLIEEVLFDELRAGGRSAAAAGPT
                     HDNFTSAFAALTTLYPEATNSRAQGQTVPYVGDLLLYFSRIYTLAGGDINLLAPGGQV
                     NVGL"
BASE COUNT          351 a          698 c          662 g          346 t
ORIGIN      
        1 atcctggcac ccggtgtgcc ggacgcgcac tccccgctcg tcgtgcgcac tggtacgggt
       61 agcatcgatc tggcggccgc cggggatctg cggctcggca atcaggcctc ggtcatctac
      121 acggcgggag cccaaggccc acccaacttc gcgaccgccc gggatggaac ttcctacctg
      181 gcgcaggaag gcggcagcgt cacggtccgc gtgggccgcg atatcgtcgg cgccaccagc
      241 gatcagcttt tcaccaactg gctgttcagg tccggtctca actcgtcaaa ccttcgcggc
      301 ggcttccagc cgaccgcttg gggcatctcc tatgacaatt tcgaacaggg tatcggcgcg
      361 ctcgggggcg gcgatctgac gatcagcgcg ggtcgtaaca tcatcgatct gggggcggac
      421 gttccctcga tcggcgtgcc ggtggggacc gcccacagcg cgacgacggt ggaggagaat
      481 cccgggacgc tgagcatcga gtccggcggc gagatcctcg ggggtaagtt cctcgacatg
      541 gccggcgtcg catccgtcgt ggcgggcggg gcgatactca ccggcagcac gcaggccatc
      601 gcgaatggct ccccgaccgg cctcaacctg atcctgggaa ccggttcagg gcagttctcg
      661 gtgacggccc gttcggacct tctgttcgat gccgcgaccg cggtgacgct cataccgcgt
      721 gcaaggacgc aggctgtcac cggcggtacg acctacttcg gcacattcac ggatgcgagc
      781 tcgctgactc tggagtccgt cggtggcacg ctggacttca cgaaccgcag cagactcatc
      841 gaggaatccg cgcccgcgca gtcgccccgt ttcgactcac aggccagcga cgaccagccc
      901 ggcctgcaca ttctcgcccc caccttcgga gcgattgcct ttggcggtga cctgcgggta
      961 agcggcagtg tggcgctctg gccgagtgct catggcaacc tcgacctcct ggctgccggc
     1021 tcggtgcagc tcaacgacgc cccggtcctc gtgtcggatg tggatccggg caggctgccc
     1081 tcagccaacg cggtctcgca aagcatcgtc cagaacgtcg atccgattct cgggctgctc
     1141 gatccgcggt cgccggacgg ggcggccacc ctctcgacgc cattcctgca cgcgccgctg
     1201 ccggtgcatg gcggagcgta cgcgagcgac ggtcagccgg atcgggtacc gatccgcatc
     1261 gtcgcgctca cgggagacgt atccctgcag ccggcgtcga acgggaccgt gagtccgatt
     1321 gacgtgccga aggcaatcga cattttcgcc ggcaaggaca tcgtcaacct ggatctcaac
     1381 gtgcagcagg acacgagcgc cgacgcttcg agcatcgtgg cgggggggaa catcacctat
     1441 cccttcaatc gaatctttac cggtgtcctg gtgccgagca acgcggcgat caacgtggcc
     1501 ggccccggaa atttcgagat tcaggcgggc ggcaccatca atctcggcac ctcgttgggc
     1561 atctcgagca tcggtaacat ctataaccct gcgctgcccg ccacgggcgc gaatctcagc
     1621 gtcgcagcgg gcatcagcgc ggcgccgcag tacccggcct tcatcagcac ctacctggcc
     1681 gacggctcga cttacgatca gcagctcgaa cagtacatag ccgataccga aggggtgcag
     1741 aaccccacca aggcgcaggc actcgcgttg ttcgaggcac tgcccaccgc gcagcaggtg
     1801 ccactcatcg aggaggtgct gttcgatgag ctgcgggcgg gagggcgcag cgcggccgct
     1861 gccgggccga cgcacgacaa cttcacgagc gcctttgcgg cgttgacgac cctatacccg
     1921 gaggccacga atagtcgcgc tcaggggcag acggtgccct atgtgggcga cctgctgctg
     1981 tacttcagcc gcatctatac gctcgctggt ggcgacatca acctgctcgc tcccggcggg
     2041 caggtgaacg tcggact
//
LOCUS       DATLKL010000312         1628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_82354, whole genome shotgun sequence.
ACCESSION   DATLKL010000312 DATLKL010000000
VERSION     DATLKL010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82354"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..429)
                     /gene="gatC"
                     /locus_tag="VM713_04085"
     CDS             complement(139..429)
                     /gene="gatC"
                     /locus_tag="VM713_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HVH83479.1"
                     /translation="MALSREQIESIAALARLELTAAELPAYQDSLSRILEFVGALERA
                     DTASVAPMAHPLPGLSQRLRPDVVTEQDHHREYQQNAPQVAAGLYLVPKVIE"
     gene            564..1607
                     /locus_tag="VM713_04090"
     CDS             564..1607
                     /locus_tag="VM713_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HVH83480.1"
                     /translation="MLKRLRGYFSSDLSIDLGTANTLIYVRDKGIVLNEPSVVAVQDE
                     PGRGGKVIVAVGTEAKSMLGRTPGHITAVRPMKDGVIADFTYTEKMLQYFINKVHGAR
                     MIKPSPRVLVCVPFGSTQVERRAIRESAEGAGARNVAIVSEPMAAAVGAGLPVHEARG
                     SMVLDVGGGTSEVAVLSLNGVVYANSARIGGDRFDEAIMNYVRRNYGILIGEATAERI
                     KIEIGSAYPGQQVRELSVKGRNLSEGVPRSFTLNSNEILEALQEPLQAIVSAVKQALE
                     QTPPELGADVAERGIVLTGGGALLRDIDKLLTEETGLPVVVADDPLTCVARGGGRILE
                     LMDELGPGHFGLE"
BASE COUNT          271 a          528 c          569 g          260 t
ORIGIN      
        1 aaggcattga gcgcgccctg gctccgctcg atgcgcccga ggaacgcctc ggtgagctcg
       61 acgctcgaga agcgccgcgc gcgcagcccg gcggcgagct cggtgaggcc gatgtgatgc
      121 agttcactca tcggcagctc actcgatcac cttcggcacc aggtacagcc ccgcggctac
      181 ctgaggcgcg ttctgctggt attcgcggtg gtggtcctgc tcggtcacga cgtcgggccg
      241 cagccgctgc gagagcccgg gcagcgggtg cgccatggga gcgacgctcg cggtatcggc
      301 gcgctccagc gccccgacga attccagaat gcgcgacagg ctgtcctggt acgcgggaag
      361 ttccgcggcg gtcagctcca gacgcgccag ggcagcgatg ctctcgatct gctcgcggct
      421 aagggccatg ggaggtaagg ctcggggtgt cggcgaactg cgaaaaatga gcggaaaatc
      481 atacatgttg ccttgctgaa tgtcctgtcg gttgctagat taccggccac tttcggggtc
      541 ttcccccact ttccggtcgc ttcatgctga aacgcctgcg cggttatttt tccagcgatc
      601 tgtcgatcga cctgggtacg gccaacacgc tcatctacgt gcgcgacaag ggcatcgtgc
      661 tcaacgagcc gtccgtggtc gcggtgcagg acgagccggg tcgcggcggc aaagtgatcg
      721 tcgccgtcgg cacggaagcg aagagcatgc tcgggcgcac accgggacac atcaccgccg
      781 tgcgtccgat gaaggacggc gtgatcgccg acttcaccta caccgagaag atgctgcagt
      841 acttcatcaa caaggtgcac ggcgcccgca tgatcaaacc ctcgccgcgc gtgctggtgt
      901 gcgtgccctt cggctcgacc caggtcgagc ggcgcgcgat ccgcgagtcg gccgaaggcg
      961 ccggggcgcg caacgtggcg atcgtgagcg agccgatggc ggcggcggtg ggcgcagggc
     1021 tcccggtgca cgaagcccgc ggctcgatgg tgctcgacgt cggcggcgga acctccgagg
     1081 tcgcggtgct gtccctcaac ggcgtggtgt acgccaactc ggcgcgcatc ggcggcgacc
     1141 gcttcgatga ggcgatcatg aactacgtgc gccgcaacta cggcattctg atcggcgagg
     1201 cgacggccga gcgcatcaag atcgagatcg gctcggccta ccccgggcag caggtgcgcg
     1261 agctgtcggt caagggccgc aatctctccg agggcgtgcc gcgcagcttc accttgaaca
     1321 gcaacgagat cctcgaggcg ctgcaggagc cgctgcaggc gatcgtcagc gcggtcaagc
     1381 aggcgctcga gcagacgcct cccgagctcg gcgcggacgt cgccgagcgc ggcatcgtgc
     1441 tcaccggcgg tggcgcgctg ctgcgtgaca tcgataagct gctcaccgag gagacgggac
     1501 tgcccgtcgt cgtggccgac gacccgctca cctgcgtggc gcgcggtggc ggccgcattc
     1561 tcgagctgat ggacgagctg gggcccgggc acttcggtct cgagtagcgc aaggagcgca
     1621 taaaccca
//
LOCUS       DATLKL010000313         3161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_71380, whole genome shotgun sequence.
ACCESSION   DATLKL010000313 DATLKL010000000
VERSION     DATLKL010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71380"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VM713_04095"
     CDS             <1..362
                     /locus_tag="VM713_04095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVH83481.1"
                     /translation="VPGFVIQGGGLTADFANKKTRAPIRNEAQNGLKNTRGSLSMART
                     SDVNSATSQFFVNLKDSGFLDHGPRDFGYAVFGRVTDGLEVIDRIASLSTGTRKGYQD
                     APLEDVVIVSARRLAPA"
     gene            399..1112
                     /gene="mtgA"
                     /locus_tag="VM713_04100"
     CDS             399..1112
                     /gene="mtgA"
                     /locus_tag="VM713_04100"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HVH83482.1"
                     /translation="MRLLRWLGAALFVWLLLTAVPVVTLRWLHPLTSAFMLEAAADAA
                     LADQRSYYTDYRWVSLERISPHAAIAVIASEDQQFPFHAGFDFDSIRDAVRASDRGKR
                     LRGASTISQQVAKNLFLWPGHSFVRKAFEAWFTVLIETLWPKERILEMYLNVAQFGDG
                     IYGVQAAAARFWHKPALSLSSDEAALLAAVLPNPLRLHAERPSAYVLERRDWILEQMR
                     ELGGAGYLREVEKERSSKH"
     gene            1126..1596
                     /locus_tag="VM713_04105"
     CDS             1126..1596
                     /locus_tag="VM713_04105"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HVH83483.1"
                     /translation="MPNTRTLEIWNLLEEFPGGKWAFSRLVCLRAPYFSSIRPLITEL
                     RPERCEVRMRKRRAVLNHIGTVHAIAMCNMAELAAGMMTDVTIPPTHRWIPKGMSVQY
                     LQKATTSLVAIAVPGGVADWSAPADYPVKVLVQDGQGHVVLAALITMRVSPKQA"
     gene            2051..>3161
                     /locus_tag="VM713_04110"
     CDS             2051..>3161
                     /locus_tag="VM713_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83484.1"
                     /translation="MSSLISPPARSARLFAVLALIILPRGGSAASEDGMSAALGAYPM
                     TREASGTAWQPDSSVHEGLHLPAGEWMVMSHALLDGVYDRQEGRRGDDKTFVGGMLMS
                     AATRVLPSEDIVRLRAMLSPEPLMGASGYPLLLATGETANGRDPLIDRQHPHNLFMEL
                     SGSFSHPLRAEESLYVYAGLPGEPAFGPPTFMHRLSILDDPAAPIAHHWLDSTHISNG
                     VVTLGYVQGDFKWEASRFYGREPDQHRYQIEPGPLDSTAARLSWNPAETLSLQISWAR
                     QISPEQLDPHADETRASGSAIYTASLGANRYWSTTAAYGYRHSTDSPHLWAYVLESTV
                     KPTELWTIFARFERESNNELIDLGGQVGATYDVGET"
BASE COUNT          520 a         1095 c         1011 g          535 t
ORIGIN      
        1 tcgtgcccgg cttcgtcatc cagggcggcg gcctgacggc ggacttcgcc aacaagaaga
       61 cccgtgcacc gatccgcaac gaggcgcaga acggcctgaa gaacacgcgc ggctcgctct
      121 ccatggcgcg caccagcgat gtcaacagcg ccacctcgca attcttcgtc aacttgaagg
      181 acagcggctt tctcgatcac ggtccgcgcg atttcggcta cgccgtattc ggccgggtga
      241 cggacggcct ggaggtcatc gacaggatcg cgagcctctc gacgggcaca cgcaagggtt
      301 accaggacgc acccctcgag gacgtcgtga tcgtctcggc ccgccgcctc gcgcccgcat
      361 aagaccggct gaccttggcg cagcggcgat ccctcaccgt gcggctgctc cggtggctgg
      421 gcgcggcgct gttcgtgtgg ctgctgctca ccgccgttcc ggtggtcact ctgcgctggc
      481 tgcaccccct cacgagcgcc ttcatgctcg aggcggcggc cgatgcggcg ctcgccgacc
      541 agcgcagcta ttacaccgat taccgctggg tgagcctcga gcgcatctcg ccccacgccg
      601 ccatcgcggt catcgcctcg gaggatcagc agttcccgtt ccacgccggc ttcgacttcg
      661 actcgatccg cgatgcggtg cgggccagcg accgcggcaa gcggctgcgc ggcgcgagca
      721 ccatctcgca gcaggtcgcc aagaatctct tcctgtggcc gggacacagc ttcgtgcgca
      781 aggcgttcga ggcctggttc accgtgctga tcgagacgct gtggccgaag gagcggatcc
      841 tcgagatgta cctgaacgtc gcgcagttcg gcgacggcat ctacggcgtg caggcggccg
      901 ccgcgcgctt ctggcacaag ccggcgctga gcctgagctc cgacgaagcg gcgctgctcg
      961 cggcggtact gccgaatccg ctgcgcctgc acgccgagcg gccttcggcc tacgtgctcg
     1021 agcggcgcga ctggatcctc gagcagatgc gcgagctcgg cggcgcgggc tacctgcgtg
     1081 aggtggagaa ggaacggtcg tccaagcact aacgagggtc acagcgtgcc gaataccagg
     1141 actcttgaga tctggaacct cctcgaggaa tttcctggcg ggaagtgggc cttctcgcgg
     1201 ctggtgtgcc tcagggcacc ctacttcagc agcattcgac cgctcatcac ggagctgcgc
     1261 ccggagcgct gcgaggtgcg catgcgcaag cgccgcgccg tgctcaatca catcggcacc
     1321 gtgcatgcga tcgccatgtg caacatggcg gagctcgccg cgggaatgat gaccgacgtg
     1381 accatcccgc cgacgcaccg ctggatcccg aaggggatga gcgtccaata cctccagaaa
     1441 gccacgacga gcctggtcgc gatcgccgtg ccgggtgggg tcgccgactg gtcggcaccg
     1501 gcggattacc cggtgaaggt gctggtgcag gacgggcaag ggcatgtggt gctcgcggcg
     1561 ctcatcacga tgcgcgtatc gccgaagcag gcatgagcgc ttagacttat gcggatgaag
     1621 ccggtgcttc attccctgac gctggcgatg ccgctgcttg cgctggctct gcagggatgg
     1681 gcggcctgtc cgatgcactc tgtgccgcct cgatccggcg cggtcgcggc tcacgagcac
     1741 tgtgcggcga accactcgtt atcgggaaca ccgcacgcgc ccaagactcc gcgcgctgcg
     1801 tgctgcgacc tcacaggatg caccggcgtc gcgctgctac ctaacccatt gccgatcgaa
     1861 gtcttcgcgg gggcggcacc ggtgagcgcc tcgctgccgc cgagctcttc tccctcacac
     1921 ttcaacggcc gcctcgaacg gccacccaag ccgatctcgc tctgaccacc cgccgttagg
     1981 cgggcagtac ccgtgcgtgc acgggcctcg gggcgcgaga cgctccgcct gtcatcgagg
     2041 tcgcagattc atgtcaagct tgatttcgcc gcctgcgcgc tcggcgcgcc tgttcgccgt
     2101 gctggcattg atcattcttc cgcgcggcgg gtcggccgcg tccgaagacg gaatgagcgc
     2161 cgcgctcggt gcctacccca tgacccgcga ggcatccggc actgcctggc agccggatag
     2221 ctcagtgcac gaaggcctgc acctgccggc aggcgagtgg atggtgatga gccacgcgct
     2281 tctcgatggc gtctacgacc gtcaggaggg gcggcgcggc gatgacaaga ccttcgtcgg
     2341 cggcatgctg atgagtgcgg ccacgcgggt gctcccgtct gaggacatcg tgcgactgcg
     2401 ggcgatgctg agccctgagc cattgatggg ggcatccgga taccccctgc tgctggccac
     2461 tggcgaaacc gcaaacggca gggatccgct catcgaccgg cagcacccgc acaatctctt
     2521 catggagctt tccggcagct tcagtcatcc gctccgagcc gaggagagcc tctacgtgta
     2581 cgcggggctg cccggcgagc ccgccttcgg ccccccgaca ttcatgcatc gcctctcgat
     2641 tctcgatgac cccgcagccc ccatcgccca ccactggctc gactccaccc acatcagcaa
     2701 cggcgtggtg accctcggct acgtgcaggg ggacttcaag tgggaggctt cccgtttcta
     2761 cggccgtgag cccgatcaac atcgctatca gatcgagcct ggacccttgg attcgaccgc
     2821 ggccagactg tcgtggaatc ctgccgagac cctgtccctg cagatatcct gggcacgaca
     2881 gatcagcccc gagcagctcg acccgcatgc cgatgagacg cgcgcttcgg ggagcgcgat
     2941 ctacacggcc tccctcggcg cgaatcgcta ttggtcgacc acggccgcct atggctatcg
     3001 tcacagcacc gacagcccgc atctttgggc atacgtgctc gagagcaccg tgaagccgac
     3061 cgaactctgg acgatctttg ctcgcttcga gcgcgaatcc aacaacgagc tcatcgacct
     3121 cggcggtcaa gtcggcgcca cctacgacgt cggtgagact t
//
LOCUS       DATLKL010000314         1723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_139042, whole genome shotgun sequence.
ACCESSION   DATLKL010000314 DATLKL010000000
VERSION     DATLKL010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139042"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VM713_04115"
     CDS             complement(<1..698)
                     /locus_tag="VM713_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HVH83485.1"
                     /translation="MLEVQGHRGARGLWPENTLAAFAGALELGVSAVELDCGMTRDGV
                     MVVTHDPDLNPDLTRGADGRFLTATGPRIVDLTWRELEAYDVGRLRPGSAYAARFPEQ
                     QAVEGERIPRLADVLALVRARGLGRVRVAIEVKTFPEEPRLTASPEALVAALRRDVEA
                     TGTLTLVSVMAFDWRVLTAARALMPQAATVALTEEQRGGDTVRVGAPEPSPWLGGLDP
                     ARFGNSVVRLVKATG"
     gene            844..>1723
                     /locus_tag="VM713_04120"
     CDS             844..>1723
                     /locus_tag="VM713_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVH83486.1"
                     /translation="MNSPRAWRALSLFLALAASAALPAAADDAKVLRIITWASYVPSE
                     VIAQFRKESGIEVQVTLSNNEEMISKLRATGGAGFDLAQPSQDRIFGAQQEFRIYKPL
                     DTGKVKLDEFVPELVDAVRKNATVDGKLYGLPYVWGTEGLIFNSSRSRIADYSDLCRP
                     ELKGRTAVRLKRPTLMAFALASGHDPFALYHDTKAYTALMEQVGKTLAACKANFRFFY
                     DNKDQLLNGVRSGEIWAAMMWDSGGWLLNRENPAIKFVNPRSGAIAWMDTFALPARGR
                     NEAGAYAWINFVMRPEI"
BASE COUNT          296 a          595 c          563 g          269 t
ORIGIN      
        1 cccgtcgctt tgacgaggcg caccaccgag ttaccgaagc gcgcggggtc gagaccgccg
       61 agccacggcg aaggctcggg cgcgccgacg cgcacggtgt cccctccgcg ctgctcctcg
      121 gtgagcgcca cggtagccgc ctgtggcatg agcgcccgcg cggccgtgag cacgcgccag
      181 tcgaaggcca tgacgctgac gagcgtgagc gtgccggtcg cttccacatc ccgacgcagc
      241 gccgcgacga gagcctcggg cgaggccgtg agccgcggct cctcggggaa ggtcttgacc
      301 tcgatcgcca cgcgcaccct accgaggccg cgcgcacgca ccagggcaag cacatcggcc
      361 aggcgcggaa tgcgctcgcc ctcgaccgcc tgctgctcgg gaaagcgcgc cgcgtaggcg
      421 ctgccgggtc gcaagcgccc gacgtcgtag gcttcgagct ccctccaggt gagatccacg
      481 atgcgcggac cggtcgcggt gagaaagcgc ccgtccgcgc cgcgcgtcag gtcgggattc
      541 aagtccgggt cgtgagtgac gaccatgacg ccgtcgcggg tcattccgca atcgagctcg
      601 accgcgctca caccgagctc gagcgccccg gcgaatgccg cgagcgtatt ctccggccac
      661 agaccccgtg cgccgcggtg gccctgcact tcaagcacgg cggcagctcc cgggagcggc
      721 ggtcacacga caagcggtgg aggcgatgtt atatttccaa gtatccgagc ccgatcagac
      781 tatctcgcca aaaacttgcc tgtcaacgcg acggcaatcg gtccgggagg ggcgttagac
      841 ccgatgaact cacctcgtgc gtggcgtgcg ctcagcctgt tcctcgccct cgcggccagc
      901 gccgcgctgc cggcggctgc tgacgatgcc aaggtactgc gcatcatcac ctgggcgagc
      961 tacgttccct cggaggtgat cgcccagttc aggaaagaga gcggcatcga ggttcaggtg
     1021 acgctctcga acaacgagga gatgatctcc aagctccgcg ccaccggcgg tgccggtttc
     1081 gacctggcgc agccctcgca ggaccggatc ttcggtgcgc agcaggagtt ccgcatctac
     1141 aagcctctcg acacgggcaa ggtcaagctg gacgagttcg taccggagct ggtcgatgca
     1201 gtgaggaaga acgccacggt cgacggcaag ctctatggcc tcccctacgt ctggggcacc
     1261 gaggggctca ttttcaattc cagccgcagc aggatcgccg actacagtga cctttgtcgg
     1321 cccgagctca agggccgcac cgcggtgcgc ctcaagcgcc cgacgctcat ggcgttcgcg
     1381 ctcgcctcgg gtcacgatcc cttcgcgctc tatcacgata ccaaggcgta cacggcgctc
     1441 atggaacagg tcggcaagac cctcgccgcc tgcaaggcga acttccgctt cttctatgac
     1501 aacaaggacc agctcctgaa cggggtgcgc tcgggggaga tctgggccgc gatgatgtgg
     1561 gactcgggtg gttggctcct caatcgcgag aatcccgcca ttaagttcgt caacccgcgc
     1621 tcgggcgcga tcgcgtggat ggacaccttc gcgcttccgg cccgcggccg caacgaggct
     1681 ggcgcctacg cctggattaa cttcgtgatg cgacccgaga tcg
//
LOCUS       DATLKL010000315         3010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_206466, whole genome shotgun sequence.
ACCESSION   DATLKL010000315 DATLKL010000000
VERSION     DATLKL010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206466"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1016)
                     /locus_tag="VM713_04125"
     CDS             complement(24..1016)
                     /locus_tag="VM713_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HVH83487.1"
                     /translation="MSGSAEALPPWIAPATRSLAAAQRSGRLPHALLIHEAPGAGGDW
                     LAHWAARLALCGHPAAAPCGSCGACRRALAWHHPDLTLVTPLEDSRQIRIEQVRDLAA
                     ELALTSHAGGYKVGVLTPADSMNRFAANALLKTLEEPPPRTLLTLVASQPSRLPPTVL
                     SRCQRLTVPAPSRAEAVAWLTEVRGPGPWDAALETVGEAPMLLLDADPEPLAQVGAET
                     RAGLEGLAAGRADPLATAERWARSELAVRLRCFENWLTERIRRASGAGNVMREVRAGP
                     YLPGAGPFLNMRGLYGLLDEVRELRTALDVPLNRTLALEALLRRLAPPGAGVAA"
     gene            complement(1013..1636)
                     /gene="tmk"
                     /locus_tag="VM713_04130"
     CDS             complement(1013..1636)
                     /gene="tmk"
                     /locus_tag="VM713_04130"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HVH83488.1"
                     /translation="MTGRFITLEGIEGAGKSTIAKFVLACLARRQIPGRLTREPGGTP
                     LAERIRQLVLERGTEPLSPVTETLLMFAARDLHLEHVIRPALARGEWVVCDRFTDATR
                     AYQGSGRGVAAELIEALAAAVHGGLTPDRTLLLDIPVSLGLARARGRAAGHADRFEGE
                     TQAFFEKVRAGYLELARRAPERFRVIDAAAPLDAVEERVERILEELR"
     gene            complement(1633..2727)
                     /gene="mltG"
                     /locus_tag="VM713_04135"
     CDS             complement(1633..2727)
                     /gene="mltG"
                     /locus_tag="VM713_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HVH83489.1"
                     /translation="MADRTQRLRRWLRRAGLAAGVLVLVLGVAATLVHDWLRREYVSP
                     GPAAAPARFQVEQGASVRNVLTRLESQGVVHNARAVLWYLRLLAEHPNVQAGTYEIPA
                     KASPEQIITMFEEGKVLLEQVTVVEGSTCGDFLELLAHEPDVTHTLAGKTPAEVMAAL
                     GHPGENPEGRFFPDTYRFAANTTDAVILALAYEKMSQALAAAWSDRSPKLPFDSPTQA
                     LILASLVEKEAQLKSERARIAGVFVARLRKGMRLQSDPTVIYGLGASYDGQVHTRDLR
                     TDTPYNTYTRAGLPPTPIALPGRESLVAAVQPDETGDLYFVASGLGDGAHHFSKTLGE
                     HNSAVQAYLARLRRQCAATHDTVPGASAHP"
     gene            complement(2720..>3010)
                     /locus_tag="VM713_04140"
     CDS             complement(2720..>3010)
                     /locus_tag="VM713_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HVH83490.1"
                     /translation="FLVEGGTLRTPLLDRWGVAGIMRQVVLREAGRAGVACEEAPLEA
                     ADLARTRELFLTSAVIGLRPVRSVDGRACELGAVTRELQRRLAPILAEPADG"
BASE COUNT          436 a         1080 c         1071 g          423 t
ORIGIN      
        1 ggtcgtgcgt tcatccgtgt ttcctatgct gcgacaccgg caccgggggg tgccagccgc
       61 cgtaacagag cctcgagcgc gagtgtgcgg ttgagcggca catcgagcgc agtccttagc
      121 tcccggacct cgtccagcag cccgtacaac ccccgcatat tcaagaacgg gccggcccct
      181 ggcaagtagg ggccagcacg cacttctcgc attacgttac ccgcgccgga tgcgcggcgg
      241 atgcgttctg tgagccaatt ctcaaagcac cggagcctga ccgccagctc gctgcgtgcc
      301 cagcgctccg cggtcgcgag cggatcggca cggccggccg cgaggccctc gagccccgcg
      361 cgcgtttccg cgccaacctg cgccagaggc tccggatcgg cgtcgaggag cagcatgggc
      421 gcctccccca ccgtctcgag cgccgcgtcc caggggcccg ggccgcggac ctccgtgagc
      481 caggcgacgg cctcggcacg ggagggtgcc ggcaccgtga gccgctggca gcggctcaat
      541 accgtcggcg gcagccgcga aggctggctt gccaccagcg tcaggagcgt gcgcggcggc
      601 ggctcctcga gcgtcttcag cagcgcgttg gcggcaaagc ggttcatgga atccgccggt
      661 gtgagcaccc cgaccttgta gccgcccgcg tgactcgtga gcgcgagctc cgccgcaagg
      721 tcgcgcacct gctcgatgcg gatctggcgc gagtcctcga gcggggtgac gagcgtgaga
      781 tccggatggt gccaggcgag cgcccggcgg catgcgccgc agcttccgca cggcgccgca
      841 gccggatgcc cgcacagggc gagccgcgcg gcccagtgtg cgagccagtc gccgccggcg
      901 ccaggggcct catgaatgag gagcgcgtgc ggcagccgcc cgctccgctg agccgcggcg
      961 agcgagcgcg ttgcgggggc gatccacggc ggcagggctt ccgcggaacc gctcatcgga
     1021 gctcctcgag gatgcgctcg acccgctcct ccaccgcatc gagcggggca gccgcatcga
     1081 tcaccctaaa gcgctccgga gcgcgccgcg cgagctcgag gtacccggcg cggaccttct
     1141 cgaagaaggc ctgggtctcg ccctcgaagc gatccgcgtg gcctgcggcg cgcccgcgcg
     1201 cgcgcgcgag cccgaggctc accggaatgt cgagcagcag cgtgcggtcg ggggtcaggc
     1261 cgccgtgtac ggcggcggcg agcgcctcga tgagctctgc agcgacgccc cgcccgctac
     1321 cctgataggc gcgcgtcgcg tcggtgaagc gatcgcacac gacccactcg ccacgggcga
     1381 gcgccggccg gatgacgtgc tcgaggtgca ggtcgcgcgc cgcaaacatg aggagcgtct
     1441 cggtcaccgg ggacaggggc tccgtccccc gctcgagcac gagctgccgg atgcgctcgg
     1501 cgagcggcgt gccgcccggc tcgcgcgtca gccgcccggg tatctgccgc cgcgccaggc
     1561 aagccagcac gaacttggcg atcgtcgact tgccagcgcc ttcgatcccc tcgagcgtga
     1621 tgaagcggcc ggtcatgggt gagcgctcgc gccgggcacg gtgtcgtgag tggcggcgca
     1681 ctgccggcgc agccgtgcga gataggcttg cactgcggaa ttatgctctc cgagcgtttt
     1741 cgagaaatgg tgcgccccgt ccccgagccc actcgcgacg aagtaaaggt cgccggtctc
     1801 gtcgggctgg accgcggcca ccagcgactc gcgtccggga agcgcgatcg gcgtcggcgg
     1861 aagacctgcg cgcgtgtagg tgttgtaggg cgtgtcggtc cggaggtcac gcgtatgaac
     1921 ctgtccatcg tagctcgccc ctagtccgta gatcacggtc ggatccgact gcagtcgcat
     1981 gcccttgcgc aggcgcgcca cgaatacccc ggcgatgcgc gcgcgctcgg acttcagctg
     2041 cgcctccttc tcgaccagcg aggcgagaat cagcgcctgg gtcggactgt cgaaggggag
     2101 cttggggctg cggtcgctcc aggccgcggc gagcgcctga ctcatcttct cgtaagccag
     2161 ggcgaggatc acggcatcgg tggtgttcgc ggcgaagcga taggtgtcgg ggaagaagcg
     2221 cccttcgggg ttctcgccgg ggtgtccgag cgcggccatc acttcggcgg gcgtcttgcc
     2281 ggccagggtg tgcgtcacgt cgggctcgtg ggccaggagc tcgaggaaat cgccgcaggt
     2341 cgagccctcg accaccgtca cctgctcgag cagcaccttg ccttcttcga acatcgtgat
     2401 gatctgctcg gggctggcct tcgcggggat ctcgtaggtg cccgcctgaa cgttcgggtg
     2461 ctcggcgagc agtcgcaggt accacagtac ggcccgcgcg ttgtgaacga ccccctggct
     2521 ctcgagccgc gtgagcacgt tgcgcacgct cgccccctgc tccacctgga agcgcgccgg
     2581 cgccgcggcc ggcccgggcg acacgtactc acgcctgagc cagtcgtgca cgagagtggc
     2641 cgccaccccg agcaccaata ccagcacgcc ggcggcgagg cccgcgcggc gcagccagcg
     2701 gcgcaggcgc tgagtgcggt cagccatcgg ctggctccgc cagaatcggc gccaggcgtc
     2761 gctgcagctc gcgggtcacc gccccgagct cgcaggcacg cccgtcgacc gagcgcaccg
     2821 ggcgcagccc gatcacggcg ctcgtgagga agagctcgcg tgtgcgcgcg agatctgcgg
     2881 cctcgagggg ggcctcttcg cacgcgacac ctgcgcgccc cgcttcacgc agcaccacct
     2941 gccgcatgat gcccgcgacg ccccaccgat cgagcagcgg ggttcgcagc gttccaccct
     3001 ccacgagaaa
//
LOCUS       DATLKL010000316         3364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_127699, whole genome shotgun sequence.
ACCESSION   DATLKL010000316 DATLKL010000000
VERSION     DATLKL010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127699"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VM713_04145"
     CDS             <1..286
                     /locus_tag="VM713_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83491.1"
                     /translation="GWPKARDCAELAPGGEPEALLALLRRLPETRIAVVGHEPGLGRL
                     VAASLPGTAAPRAFRFKKMGAALLTFPGGARVRSARLDWLVPAKVLRAAR"
     gene            complement(315..1907)
                     /locus_tag="VM713_04150"
     CDS             complement(315..1907)
                     /locus_tag="VM713_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003850384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HVH83492.1"
                     /translation="MSDASSDKPLAAPPPRAAAELAPLTGSKLAIGTIALSLATFMNV
                     LDTSIANVSIPAIAGDLGVSPDQGTWVITSFGVANAISLPLTGWLTRRFGQVKLFTAS
                     VLLFVIASFLCGLAPSLSALIVFRVLQGAVAGPMIPLSQSILLTSYPKSRAGTALAMW
                     AMTTLVAPVVGPLLGGWITDNISWPWIFYINIPVGVGAALATWAIYHKRETPTARVPV
                     DTVGLGLLVIWVGSLQIMLDQGKDLDWFNSGAIATLACVAAVAFAIFLVWELTEEHPI
                     VDLTLFARRNFWVSTVALLMGYGLYFGNVVLLPLWLQQYMGYTATLAGLVLAPVGLLA
                     LVFTPYIGRIVHRVDPRILVTISFAIFALVFFMRANFTTEASLWTLLVPTLIQGAGAA
                     TFFIPLLTMTLSGLPPERIPNASGLSNFARITAGSFGTSITTTLWERRASLHHAQLVQ
                     HLTPNDPAAAQTFANLQASGLGNEQSYALLNRLVDGQAFMLSADEIFYASGIIFIVMI
                     GLVWLARPQLGAAGGAAASGAH"
     gene            complement(1920..3179)
                     /locus_tag="VM713_04155"
     CDS             complement(1920..3179)
                     /locus_tag="VM713_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVH83493.1"
                     /translation="MSTNNPKTPAISRRWRLLAAVGAGVLAVALGYGLYWAQVLRYHQ
                     TTDDAYVSGNVVQITPQISGTVVAIGADDTQFVKAGQSLVRLDRADAKVALDQAEAQL
                     ARTVRDVRNQFATSAQLEAAVDAKQTDLTRAQNDLERRARLGASGAVSGEELQHARDT
                     VKAAQADLNAARQQLAANRARIDGTTLQDHPEVRDAAAAVRSAYLTWVRTELPAPVSG
                     FIARRNVQLGQRVAPGTPLMAVVPLDQVWVDANFKEPQLSRMRVGQPVTLSADLYGGR
                     VVFHGKVAGFGAGTGAAFSLLPARNATGNWIKIVQRVPVRVALDPRELAEHPLQIGLS
                     MRVDVDVHDASGARLSQLASTATPWSTDVFGSTDAQADARVAAIISANEPAAARASVT
                     PTSPVRGPGLLADAHSVVAGAGHAKAP"
BASE COUNT          562 a         1102 c         1198 g          502 t
ORIGIN      
        1 cggatggccc aaggcaaggg actgcgcgga gctcgccccg ggcggagagc ccgaggctct
       61 gctcgccctc ttgaggcgct tgcccgagac gcggatcgcg gtcgtcggac acgagccggg
      121 actcgggcgg ctggtcgcgg catcgcttcc cggaaccgcg gcgccgcggg cgttccgctt
      181 taagaaaatg ggcgcagcgc ttctcacctt cccgggcggg gcgcgcgtca gaagcgcgcg
      241 gctcgattgg ctggtgcctg caaaagtctt gcgcgccgcg cgttagctta gcggcggctc
      301 gcgggctcgc cgctttagtg ggcgccggac gctgccgcgc cgcccgcggc accgagctgc
      361 gggcgcgcga gccacacgag cccgatcatc acgatgaaga tgattcccga ggcgtagaag
      421 atctcgtccg ccgagagcat gaacgcctgg ccatcgacca ggcggttgag gagcgcatag
      481 ctctgctcgt tcccaaggcc gctcgcctgc aggttcgcga aagtctgcgc agcagccggg
      541 tcattcggcg tcaggtgctg aacgagctgc gcgtgatgca ggctcgcccg ccgctcccag
      601 agcgtcgtcg tgattgaggt gccgaaggag ccggcggtga tgcgcgcgaa gttcgagagt
      661 cccgaggcat ttggtatgcg ctcgggcggc agccccgaga gcgtcatcgt gaggagcggg
      721 atgaagaagg tggcggcgcc tgcgccctga atgagcgtcg gcacgaggag cgtccacagg
      781 ctcgcctcgg tcgtgaaatt cgcgcgcatg aagaagacca gcgcgaagat cgcgaacgag
      841 atggtcacca gaatacgcgg atcgacgcga tggacgatcc gtccgatgta gggtgtgaac
      901 accaaggcga ggagcccgac cggcgccagc accagtcccg cgagcgttgc ggtgtagccc
      961 atgtactgct gcagccacag cggcagcagc accacgttgc cgaagtagag cccgtaaccc
     1021 atgaggagag cgaccgtgct cacccagaaa ttgcgccggg cgaagagcgt caggtctacg
     1081 atcgggtgct cttcggtgag ctcccagacg aggaagatcg caaaggcgac cgccgccacg
     1141 cacgcgagcg tcgcgatcgc cccggagttg aaccagtcga ggtccttgcc ctggtcgagc
     1201 atgatctgca gcgacccgac ccagatgacg aggagcccga ggcccaccgt gtcgaccggc
     1261 accctcgccg tcggcgtctc gcgcttgtga tagatcgccc aggtggcgag cgcggctccg
     1321 acacccaccg ggatgttgat gtagaaaatc cacggccagg agatgttgtc ggtgatccag
     1381 ccgccgagga gcggccccac caccggcgcg accagcgtcg tcatcgccca catggcgagc
     1441 gccgtgccgg cgcggctctt cgggtagctc gtgagcagga tcgactgcga gagcggaatc
     1501 atgggacctg cgaccgcgcc ctgcagcacg cggaacacga tgagcgccga caggctcggc
     1561 gcaagcccgc acagaaacga ggcgatcacg aatagcagga ccgacgctgt aaagagcttc
     1621 acctgaccga agcggcgcgt cagccagccg gtgagcggga gcgagatcgc gtttgcgacg
     1681 ccgaaggagg tgatcaccca ggtgccctga tcgggtgaga cgccgaggtc gccggcgatc
     1741 gccggaatgg acacgttcgc gatcgacgtg tcgagcacat tcatgaaggt cgcgagcgag
     1801 agtgcgatcg tgccgatcgc gagctttgag cccgtgaggg gcgcgagctc ggccgccgcc
     1861 cgcgggggcg gcgcggcaag aggcttatcc gagctcgcgt cggacatcgt cacgagccct
     1921 cagggcgcct tggcgtgccc ggcgcccgcc acgaccgaat gcgcgtccgc aagaagcccc
     1981 gggccgcgga ccgggctcgt cggggttacc gaggcgcgcg ccgcggccgg ctcgttggcg
     2041 ctgatgatcg ccgccacgcg ggcatcggcc tgcgcatcgg tcgagccgaa cacatccgtc
     2101 gaccagggtg tcgcggtgct cgcgagctgc gagaggcgcg cgccgcttgc atcgtgcacg
     2161 tcgacgtcga cccgcatgga gagcccgatc tgtagcgggt gctctgcgag ctcacgcgga
     2221 tcgagtgcga cgcgcaccgg cacgcgctgc acgatcttga tccagttgcc ggtggcattc
     2281 cgggccggga ggagcgagaa cgcggcgccc gtgccggcgc caaatccggc caccttgccg
     2341 tggaacacca cgcggccgcc atagagatcc gcgctgagcg tcaccggctg tccgactcgc
     2401 atgcgcgaga gctgcggctc cttgaagttc gcatcgaccc acacctggtc gagcggcacc
     2461 accgccatga gcggcgtgcc gggggcgacc cgttgcccga gctgcacgtt gcggcgggcg
     2521 atgaagcctg aaacgggtgc cggcagctcg gtgcgcaccc aggtgaggta ggcgctgcgc
     2581 accgcggcgg cggcgtcacg cacctcgggg tggtcctgga gagtcgtgcc atcgatgcgg
     2641 gcgcggttag cggcgagctg ctgccgggcg gcattgagat ccgcctgcgc agctttcacc
     2701 gtatcgcgcg cgtgctgcag ctcctcgcct gagacggcac cggaggcccc aagccgtgcc
     2761 cgccgctcga gatcgttctg cgcccgggta aggtccgtct gcttcgcatc gactgcggct
     2821 tcgagctgcg cgctggtcgc gaactggttg cgcacgtcac gcacggtgcg cgcgagctgc
     2881 gcctcggcct gatcgagcgc caccttggcg tcggcgcgat cgaggcgtac cagcgactgt
     2941 cccgccttca cgaactgagt gtcgtcggcg ccgatcgcga cgacggtccc ggaaatctgc
     3001 ggcgtgatct gcacgacgtt gccactcacg tacgcatcgt ccgtcgtctg gtgatagcgc
     3061 agcacctgtg cccaatagag gccgtacccg agcgcgaccg cgagcactcc ggctcccact
     3121 gcggcgagca ggcgccagcg gcgggagatc gcaggggtct tggggttatt ggtgctcatg
     3181 gcattcctta tcgggaaatc cgtgttgaac tgaaatatcg gtccgtcgtt ggtcaggtca
     3241 ggcgctcgcc cggaccgccg gcgatccggc gttctcgagc atgcgcgtca ggaggctttc
     3301 gagctgccgc gcctccgtct tggaaaagcc gtggaggaag cggttcgcga cgacgagtgc
     3361 gagc
//
LOCUS       DATLKL010000317         1941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_139057, whole genome shotgun sequence.
ACCESSION   DATLKL010000317 DATLKL010000000
VERSION     DATLKL010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139057"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..592)
                     /gene="rfbC"
                     /locus_tag="VM713_04160"
     CDS             complement(44..592)
                     /gene="rfbC"
                     /locus_tag="VM713_04160"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HVH83494.1"
                     /translation="MRATPLPLPGVLLIEHQVFDDARGAFFESWNERDFAAAGIEARF
                     VQENVSRSGAYVLRGLLYQIRHSQGKLVRVLAGEVFDVVVDLRRSAPTFGKSCTVQLG
                     AASARSLWVPAGFAHGFLALADETHVQYKVTDFWVREAERTLQWNDAALGIRWPQPAG
                     VEPLLSAKDRDGTPLARAECFP"
     gene            complement(592..1473)
                     /gene="rfbA"
                     /locus_tag="VM713_04165"
     CDS             complement(592..1473)
                     /gene="rfbA"
                     /locus_tag="VM713_04165"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HVH83495.1"
                     /translation="MITKGIVLAGGSGTRLYPVTRAVSKQLLPLYDKPLVYYPLATLM
                     LAGIRDVLLISTREDAPLFERLLGDGSQWGMRVCYAVQPEPRGIAQALLIGADFIGGD
                     PVCLVLGDNLFYGHGLPEQLRAATARDRGATVFAYYVRDPERYGVVEFDSAGRAVGLE
                     EKPAAPRSHYAVTGLYFYDAECVAAARALQPSARGELEITDVNRAYLTRGMLQVEVLG
                     RGVAWLDTGTHASLLAAANFVETVEARQGLKICCPEEIAYRQGFIDRAQLARLAAPLK
                     RTGYGEYLEGVLKERVF"
     gene            1561..1803
                     /locus_tag="VM713_04170"
     CDS             1561..1803
                     /locus_tag="VM713_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83496.1"
                     /translation="MRASRWVAAAALTLAALAALSSAVADPPVDPKRRAKASSFAPHH
                     TKRHVYGAPIAKPILHKRNKPTPPAPGTSTAAPPVK"
BASE COUNT          323 a          674 c          678 g          266 t
ORIGIN      
        1 agcgcgcgac cgagctggcc cgaagagccc gtgatgagca ctctcacgga aagcactccg
       61 cccgggcgag cggcgtgccg tcacgatcct tcgccgacag gagcggctcg acccccgcgg
      121 gctgcggcca gcggatgccg agcgcggcat cgttccactg gagggtgcgc tccgcctcgc
      181 gcacccagaa gtcggtgacc ttgtactgca cgtgcgtctc gtcggccagc gcgagaaacc
      241 cgtgcgcgaa gccggcgggc acccacagcg agcgcgcgct ggcggcgccg agctgtaccg
      301 tgcagctctt gccgaaggtc ggagcggagc ggcgcaggtc caccacgacg tcgaagacct
      361 ctccggcgag cacccgcacg agctttccct gggagtgcct gatctggtag aggagtcccc
      421 gcagcacgta cgcccccgag cggctcacgt tctcctgcac gaagcgcgcc tcgatgccgg
      481 ccgccgcgaa gtcgcgctcg ttccagctct cgaagaaggc gccgcgcgca tcatcgaaca
      541 cctggtgctc gatcagcagc acgcccggta gcgggagcgg cgtggcgcgc atcagaacac
      601 ccgctccttg agcacgccct cgaggtattc gccgtagccg gtgcgcttca agggtgcggc
      661 caggcgcgcc agctgcgcac ggtcgatgaa gccctgacgg taggcgatct cctccgggca
      721 gcagatcttg agcccctgcc gcgcctcgac cgtctcgacg aagttggcgg ccgcgaggag
      781 cgaggcatgg gtgccggtgt cgagccaggc gacgccgcgg cccaggacct cgacctggag
      841 catgccgcgc gtgaggtagg cgcggttgac gtcggtgatc tcgagctcgc cgcgcgccga
      901 tggctgcagg gcacgcgccg ccgcgacgca ctcggcgtcg tagaagtaga ggccggtcac
      961 cgcataatgc gagcgcggcg cggccggctt ctcctcgagc cccacggcgc ggcccgcgga
     1021 gtcgaattcc acgacgccgt aacgctccgg atcgcgcaca tagtaggcga agacggtcgc
     1081 accgcggtcg cgcgccgtgg cggcgcgcag ctgctcgggc aggccgtgtc cgtagaagag
     1141 gttgtcgccg agcaccaggc acaccgggtc gccgccgatg aagtcggcgc cgatcaggag
     1201 cgcctgcgcg atgccgcgcg gctcgggctg caccgcgtag cagacgcgca tgccccattg
     1261 cgagccgtcc ccgagcagcc gctcgaacag cggagcgtcc tcgcgcgtcg agatgagcag
     1321 cacgtcgcgg atgccggcca gcatcagcgt cgccagggga tagtagacga gcggcttgtc
     1381 ataaagcggc agcagctgct ttgagaccgc ccgcgtgacc ggataaagtc gggtgccgga
     1441 gccaccggcg aggacgatgc ctttggtgat catggtgcct tgtcagtcag agcgggcggc
     1501 gcatcctagc gtgtcgccgt gggcgccacc aagcagcggc ggggcaagcg ggtggctgtc
     1561 atgagagcgt cacgctgggt cgcggcggcg gcgctgacac tggctgcact cgcggcgctg
     1621 agctcagcag tcgcggatcc gcccgtcgat cccaagcgaa gggccaaggc gagctccttc
     1681 gcgcctcatc acaccaagag gcacgtgtac ggcgcgccga tcgcgaaacc gatcctgcac
     1741 aagcgtaaca agcccacccc gcccgcgccc ggcacgagca cggcggcccc gccggtcaag
     1801 taggcgtgcg tgccgaccgt gcattagagt ggccgcgtgc tcactgcggc cgaactcgac
     1861 acaaatcgat caggcgttca tgttcgagga gattgtgggc tcgtcaccgg ccctgcaaac
     1921 cgtcctctcc agcattgtga a
//
LOCUS       DATLKL010000318         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_59990, whole genome shotgun sequence.
ACCESSION   DATLKL010000318 DATLKL010000000
VERSION     DATLKL010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59990"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2043)
                     /locus_tag="VM713_04175"
     CDS             complement(<1..>2043)
                     /locus_tag="VM713_04175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HVH83497.1"
                     /translation="ERLTDVRLDGVPVSAVESDDAQQLTTVRLAQAAPAGEHTLSFSY
                     TGRLETQPHGLFVQPYLTSDGSQRRLLSTKMESTDARRMFPCWDEPAFRATFELTVTV
                     PAAWATVSNMPVARRELHGDQATTTFERSPSMPSYLIEFTGGDLAALNGRAGDTGLGI
                     WTVRGREPDGRVALADAALILADYNDYFGYSFPLPKLDSLAIPGGFSGAMENWGAITY
                     NDQLLLLGANSTLADRQTVFAVQAHEIAHQWNGDLVTMGWWDGLWLNESFAAWRGASE
                     TDRRNPAWKWWERQDGAKESAMRADSRQSTHPIQQPVADELQAANAFDEITYEKGQSV
                     LRMLENYLGPDLFRDGIRRYLRARALSNATAADLWSALAAASGQDVSHLARDWTEQAG
                     FPLVSVAASCAHGTRTLTLTQQRFLLQRAARDGGHWSIPLRIRVGDEVPRAELLTRDG
                     QQSSGGRCNEPLSINADAIGFYRAKYDAATLAANTRAFGTLPDGDRIALLDDGWALAL
                     AGEQPLGNYLALAAAMGKDRDSRAWEQIAASLGTLEHYARGSRGNAAFTAAATALVRP
                     VADGLGWDGRPGETPDVARLRRTLLADLGAWDDRKVLAEARRRFARFTGDRSAIPPDD
                     QDTVLAIAMQAADAASFEQLHAIARAEADETAQRRDYAALMQVGDPALAARAAQIAL"
BASE COUNT          283 a          716 c          730 g          314 t
ORIGIN      
        1 agcgcgatct gcgcggcccg cgcggcgagc gccgggtcac ccacctgcat cagcgccgca
       61 tagtcgcgcc gctgtgcggt ctcatccgcc tcggcacgcg cgattgcatg cagctgctcg
      121 aagctcgcgg catccgccgc ctgcatcgct atggcgagca ccgtgtcctg gtcgtccgga
      181 gggatagcgc tgcggtcgcc ggtgaagcgg gcgaagcgcc ggcgcgcctc ggcgaggacc
      241 ttccggtcgt cccaggcgcc gagatcggcg agcagcgtgc gccgcagtcg cgcgacatcc
      301 ggcgtctcac ccggccgccc gtcccatccg aggccatcgg ccactggcct caccagcgct
      361 gtcgccgccg cagtgaatgc cgcgttcccg cggctgccgc gcgcgtaatg ctcgagcgta
      421 ccgagagagg cggcgatctg ctcccaggcg cgggagtcgc gatctttccc catggcggcg
      481 gccagtgcca ggtagttgcc gagcggctgc tccccggcga gcgcgagcgc ccagccgtca
      541 tcgagcagcg caatccggtc gccgtcgggc agggtgccga aggcgcgggt gttggccgcg
      601 agcgtcgccg catcgtattt cgcgcgatag aagccgatgg cgtcggcgtt gatgctcagc
      661 ggctcgttgc agcgaccgcc gctgctctgc tgcccatcgc gggtcagaag ctcagcgcgc
      721 ggcacctcat cgccgacgcg gatccgcagc ggaatgctcc agtgcccgcc atcacgcgcg
      781 gcgcgctgca gcaggaagcg ctgctgcgtc agcgtgagcg tgcgcgtgcc gtgcgcgcag
      841 ctcgccgcga cactgaccag cgggaagcca gcctgctcgg tccagtcacg cgctaggtgg
      901 ctgacgtcct ggccgctggc cgcggcgagc gcgctccaca agtctgccgc ggtggcgttc
      961 gacagtgcgc gggcgcgcag atagcgccgg atgccgtcgc ggaacaggtc cggtccgagg
     1021 tagttctcga gcatccgcag caccgactgt cccttctcgt aggtgatctc gtcgaaggca
     1081 ttggcggcct gcagctcgtc cgccaccggc tgctggatgg gatgggtgga ctgccgggag
     1141 tccgcgcgca tggcgctctc cttggcgccg tcctgccgtt cccaccactt ccacgccggg
     1201 ttacgccggt cggtctcgga ggcaccgcgc caggcggcga agctctcgtt gagccacagg
     1261 ccgtcccacc aacccatggt cacgagatca ccgttccact gatgcgcgat ctcgtgcgcc
     1321 tgaaccgcaa ataccgtctg gcggtcggcg agcgtgctgt tggcgccgag gagcagcagc
     1381 tggtcgttgt aagtgatggc gccccagttc tccatcgctc ccgagaagcc gccgggaatc
     1441 gccagcgagt cgagcttcgg cagcgggaag gaatagccga agtagtcgtt gtaatccgcg
     1501 aggatcagcg cggcgtcggc gagcgccacc cggccgtcgg gctcgcgtcc gcgcacggtc
     1561 cagatgccaa gcccggtgtc gccggcgcgc ccgttgagcg ccgccaggtc gccgccggtg
     1621 aactcgatca ggtaggacgg catgctgggc gagcgctcga aggtcgtggt cgcctgatca
     1681 ccgtgcagct cgcgcctggc gaccggcatg ttcgacacgg tcgcccacgc ggccggcacg
     1741 gtcacggtga gctcgaaggt cgcgcggaag gcgggctcgt cccagcaggg gaacatacgc
     1801 cgcgcgtcgg tgctctccat cttggtggac aggagccgtc gctgcgagcc gtcggaggtc
     1861 aggtacggtt ggacgaacaa gccgtgcggc tgcgtctcga gcctgccggt gtaggagaag
     1921 ctgagcgtgt gctcgccggc cggcgccgcc tgcgcgagcc tcaccgtggt gagctgctgc
     1981 gcgtcgtcgg actcgaccgc gctcaccggc acgccgtcga gacgcacgtc ggtgagccgc
     2041 tcg
//
LOCUS       DATLKL010000319         3607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_161426, whole genome shotgun sequence.
ACCESSION   DATLKL010000319 DATLKL010000000
VERSION     DATLKL010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161426"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..646)
                     /locus_tag="VM713_04180"
     CDS             complement(8..646)
                     /locus_tag="VM713_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83498.1"
                     /translation="MRVRRHPTRGGDNLRRALAQEAARIMAEHGVQDFLVAKRKAAER
                     LGVTDGAAVLPKNREIELALAEYQRLFGGESHVERLGAQRRAALAAMRRLSEFEPRLV
                     GAVLAGTATAHSDVQLHLFADRAESVTLKLIDAGIAHEVTERRVKLDAERVRPFPGVR
                     FAVDDHSIEATVFPADGIRQAPVSPVDGRPMRRANAFEVEALLGEKPGERLT"
     gene            complement(651..3128)
                     /locus_tag="VM713_04185"
     CDS             complement(651..3128)
                     /locus_tag="VM713_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HVH83499.1"
                     /translation="MTLAAGAVMLVTFLGAYALACAYLYLVPTLPSTDAMRNAELQVP
                     LRVFTRSGELIAQIGEQRRLPVSYAEIPELVKHAFVAAEDERFFEHHGIDYFGVIRAA
                     VVDVISGHKTQGASTITMQAARNMFLSLDKTARRKLQETFVTYRMEHEFTKQEIFGLY
                     LNVIFFGQRSYGVAAASEAFFGKTLDKLDVAEAATLAGVPKAPSSYNPIVNPEAATAR
                     RAYVLRRMRELGYIDTATAEAANKEPMRARAHAPLFDVEAPYVAEMARLELRQRFGAS
                     AETAGYKVYTTLDGRLQAAAVRAVRIGLIEYDRRHGWRGPAGHIELPATGAPDYDDLV
                     DEYASIGALAPAVVTSVEERGVHAYVKAVGPVTIDWGGLSWARKAQPNEAVGPTPSRA
                     AQVLKKGDVVYVVADQAGHAQLAQVPEAQSALVALDPNDGSIVALVGGFDYFTNKYNR
                     AIQARRLPGSGFKPFLYSAALERGITPASTFLDAPIVLEGNGAEEAWRPENDSRQFGG
                     PTRLRDALVHSRNLVSIRVLKEVGVDSAIDYISHFGFDPKTMPHDLTLALGTLDVTPL
                     ELASGYAVFANGGYRVQPYFIDRIEDAANQVVWRAAPRMVCRQCDEGAAPPSGATAVK
                     VANTAGGAGTFPGSDPRTSAAPSGSANGTRGEPAALPAAQVAPRVITAQNAYVMTDMM
                     SDVIRHGTGRRALALGRDDLAGKTGTTNKAVDTWFNGFTENLVATVWVGFDQEHPLGE
                     AEEGARTALPIWITFMREALRGVPEERRAMPDGIVSLRISPQTGELVSDENPSGVTEI
                     FLANHLPQNEPGMMQSAEGQQTGEPIF"
     regulatory      3205..3292
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3425..>3607
                     /locus_tag="VM713_04190"
     CDS             3425..>3607
                     /locus_tag="VM713_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83500.1"
                     /translation="MDKTQRHWLTEDSATSGRDAIPDSQSPVRTDARFRETAHVQFNF
                     ALLYSDIRNLLGGHFQA"
BASE COUNT          535 a         1239 c         1243 g          590 t
ORIGIN      
        1 cccggcgcta ggtgagcctc tccccgggct tctccccgag cagcgcctcg acttcgaagg
       61 cgttggcgcg ccgcatcggg cgcccatcga ccgggctcac gggcgcctgg cggatgccgt
      121 cggcggggaa cacggtcgcc tcgatcgagt gatcgtcgac cgcaaagcgc acgccgggga
      181 aggggcgcac gcgctcggcg tcgagcttca cgcgccgctc ggtgacctcg tgggcgatgc
      241 ctgcgtcgat cagcttcaac gtgaccgact cggcgcggtc ggcaaagaga tgcagctgca
      301 cgtcggaatg cgcggtggcg gttcctgcga gcaccgcgcc caccaggcgc ggctcgaact
      361 cgctcaagcg ccgcatggcc gcgagcgccg cgcggcgctg cgcgcccaag cgttcgacgt
      421 gcgattcccc accgaagagc cgctggtact cggcaagcgc gagctcgatt tcgcggtttt
      481 tcggcagcac cgcggccccg tcggtcacgc cgaggcgctc ggcggccttg cgcttggcga
      541 cgaggaagtc ctgcacgcca tgctcggcca tgatgcgcgc ggcctcctgc gccagtgcac
      601 gccgcaggtt gtcgccgccg cgggtcggat gtcgcctcac tcgcatgggt tcagaagatc
      661 ggctcgccgg tctgctggcc ctcggcgctc tgcatcatgc cgggctcgtt ctgcggcagg
      721 tgattcgcca ggaagatctc ggtcaccccg ctcggattct catcgctcac gagctcgccg
      781 gtctgcggcg agatgcgcag gctgacgatc ccgtcgggca tcgcgcgccg ctcttcaggc
      841 acgccgcgca gcgcctcgcg catgaaggtg atccagatcg gcagcgcggt gcgcgcgccc
      901 tcctcggcct cgccgagcgg atgctcctgg tcgaagccca cccacacggt cgcgacgaga
      961 ttctccgtga agccgttgaa ccaggtgtcc accgccttgt tggtggtgcc ggtcttgcct
     1021 gcgaggtcat cgcgcccgag cgcgagcgcg cgccggccgg tgccgtggcg gatcacgtcc
     1081 gacatcatgt cggtcatgac gtaggcgttt tgcgcagtga tgacgcgcgg cgcgacctgc
     1141 gccgcgggca gcgccgccgg ctcgccacgc gtgccattgg cgctcccgct gggcgcggcg
     1201 ctggtgcgcg ggtcgctccc cgggaaagtg ccggccccac ccgccgtgtt ggccaccttg
     1261 accgcggtgg cgccggatgg cggcgcagcg ccctcgtcgc actggcggca caccatgcgc
     1321 ggcgccgcgc gccacaccac ctggttggct gcgtcctcga tgcgatcgat gaagtagggc
     1381 tgcacgcggt agccgccgtt ggcgaacacg gcgtacccgc tcgccagctc gagcggtgtc
     1441 acgtccagcg tgccgagcgc cagcgtcagg tcatggggca tggtcttcgg atcgaagccg
     1501 aaatggctga tgtagtcgat ggcgctgtcg acgccgacct ccttcagcac gcggatcgag
     1561 acgaggttgc gcgaatgcac cagcgcatcg cgcaggcgcg tcggcccgcc gaactggcgc
     1621 gagtcgttct ccggccgcca ggcttcctcg gcgccgttgc cctcgaggac gatcggcgca
     1681 tcgaggaagg tgctcgccgg ggtgatgccg cgctcgagcg ccgcggagta caggaacggc
     1741 ttgaagcccg agccggggag ccggcgcgcc tggatggcgc ggttgtactt gttggtgaaa
     1801 tagtcgaagc cgccgaccag cgcgacgatg ctgccgtcgt tgggatcgag cgcgaccagg
     1861 gcgctctgcg cctcgggcac ctgagcgagc tgcgcgtggc cggcctgatc cgccaccacg
     1921 tacacgacgt cgcctttctt cagcacctgt gcggcccggc tcggcgtcgg cccgacggct
     1981 tcgttgggct gcgccttgcg cgcccacgag agtccgcccc agtcgatggt cacgggcccg
     2041 accgccttca cgtaggcatg cacgccgcgc tcttcgaccg aggtcacgac cgcgggcgcg
     2101 agggcgccga tcgaggcgta ttcatcgacc agatcgtcgt agtcgggcgc gcccgtcgcg
     2161 ggcagctcga tgtgtccggc ggggccgcgc cagccgtgac ggcggtcgta ctcgatgagc
     2221 ccgatgcgca ccgcgcgcac cgcggcggcc tgcagccgcc catcgagcgt cgtgtagacc
     2281 ttgtagccgg cggtctcggc gctcgcgccg aagcgctgcc gcagctcgag ccgcgccatc
     2341 tctgccacat agggcgcctc gacgtcgaag agcggcgcgt gcgcgcgcgc gcgcatcggc
     2401 tccttgttgg cggcttccgc ggtcgcggtg tcgatgtagc cgagctcgcg catgcgccgc
     2461 agcacgtagg cgcgccgcgc cgtggccgct tcaggattca cgatcgggtt gtagctcgag
     2521 ggggccttcg ggacgccggc gagagtggcc gcctcggcca cgtcgagctt gtcgagcgtc
     2581 ttgccgaaga aagcctcgga cgccgccgcc acgccgtagg agcgctgccc gaagaagatg
     2641 acgttgagat agagcccgaa gatctcctgc ttggtgaact cgtgctccat gcggtaggtg
     2701 acgaaggtct cctggagctt gcggcgcgcg gtcttgtcga ggctcaggaa catgttgcgc
     2761 gccgcttgca tggtgatggt gctggcgccc tgggtcttgt ggccggagat cacgtcgacg
     2821 accgccgcgc ggatcacgcc gaagtaatcg atcccatggt gctcgaagaa gcgctcgtcc
     2881 tcggcggcga cgaacgcatg cttgacgagc tcggggatct cggcgtagct gaccgggagg
     2941 cgccgctgct caccgatctg cgcgatcagc tcgcccgagc gcgtgaagac gcgcaaaggc
     3001 acctgcagct ccgcgttgcg catcgcatcg gtcgaaggga gcgtgggcac caggtagagg
     3061 taagcgcacg cgagtgcgta ggcgccgaga aatgtgacca gcatcacggc cccggcggcg
     3121 agggtcataa tccgaaagta attccatttc acagaaacca accctctctg cttgcgcaca
     3181 ctcggtgcgc tatgcatagt agccgccgca tcagggcaaa ctcaccgaaa ggtggggacg
     3241 caaagcctcc ggtctacggg acgtacgcgc ctatgacagc ggggttgccg gtcggaacgt
     3301 taacgcactc cgctgcgcag ggccacgcgg tcctcaaggg cagcaccgtt cgaccgctct
     3361 ccgttctccg cgcgcgacgc acttcccgca gccctgaggc ttcagctgtc ccttcacgac
     3421 acgcttggac aaaacgcagc gccattggtt gaccgaagac tccgcgacga gcgggcgtga
     3481 cgccattcct gattcacagt ctccagtgcg caccgacgcg cggttccgtg agaccgctca
     3541 cgttcaattt aactttgcgc tgctatatag tgacatccga aatctactgg gcggtcattt
     3601 tcaggcc
//
LOCUS       DATLKL010000320         1913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_60024, whole genome shotgun sequence.
ACCESSION   DATLKL010000320 DATLKL010000000
VERSION     DATLKL010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60024"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VM713_04195"
     CDS             complement(<1..774)
                     /locus_tag="VM713_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH83501.1"
                     /translation="MSYSALALLRGALSGQRHWQPAWASPEPKPAYDVVIIGGGGHGL
                     AAAYYLAKNHHVTRVAVLERGWLGGGNTGRNTTVVRSSYLFPESARIYDFSLQLYEGL
                     SRELNFNVMLSQRGHVMLAHSRHDLESMSRWVNTMHMHGIDAELLSREEVGARAPALD
                     LSPRARLPVLGGFLQPRAGTARHDAVAWGYARAASRLGVDIIQECEVTGFVRNGAGRV
                     TGVETTRGRISAERVAIAAAGHSSVLAGLAGCRLPVTSYA"
     gene            complement(785..>1913)
                     /locus_tag="VM713_04200"
     CDS             complement(785..>1913)
                     /locus_tag="VM713_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HVH83502.1"
                     /translation="ARARSKVKSAKAKSATAKSAARAVQRVGKGNGKERDLLARLREG
                     AVICAEGYVFELERRGYLQAGAFVPEVLFEHPEVVEQLHLDFVHAGSDVTQALTYYVH
                     REKLRIIGREKDLAPMNRAALRIAKSVARRTGTLFAGDLCNTNIYDPNDARAIKEVER
                     IFDEQVGWAVEAGVDYFVAETLAWVGEAELALKAIKRQAKVPTVITFAMHQEPILRDG
                     LTPAEACRRMEQAGADVVGLNCHRGPATMMPMIRDIRQAVKCHVAALPVPYRTTSQQP
                     SFMSLTDPCCNNQRAFPVGLDPFMATRSEIFEFGREAFALGVRYLGVCCGAGPHHIRA
                     LAESLGRRPPASRYTVDMSKHGIFGTDKRIRPIQTVYRDKA"
BASE COUNT          275 a          684 c          646 g          308 t
ORIGIN      
        1 cgcgtagctg gtgacgggca gccggcagcc ggcgagcccg gcgagcaccg agctgtgacc
       61 cgccgcagcg atggcgacgc gctcggcgct gattctgccg cgcgtcgtct cgacgccggt
      121 gacccggccg gccccgttgc gcacgaagcc cgttacctcg cactcctgga tgatgtcgac
      181 gccgagcctc gaggccgcgc gcgcgtatcc ccaggcgacc gcgtcgtggc gcgcggtccc
      241 ggcgcgcggc tgcaggaatc ctccgagcac cggcaggcgc gcccgcggcg acaggtcgag
      301 cgccggcgcc cgcgccccga cttcctcgcg gctcagcaac tcggcgtcga tgccgtgcat
      361 gtgcatggtg ttgacccagc gcgacatcga ctcgaggtcg tgacggctgt gcgccagcat
      421 cacgtggccg cgctggctca gcatgacgtt gaagttgagc tcccgcgaga gcccctcgta
      481 cagctgcagc gagaagtcgt agattcgcgc gctctccggg aacaggtagg acgagcgcac
      541 cacggtggtg ttgcggccgg tgttgccgcc gccgagccag ccgcgctcga ggacggcgac
      601 gcgcgtcacg tggtgattct tcgccaggta gtaggcggcc gcgagcccgt gacctccccc
      661 gccgatgatc accacgtcgt aggcgggctt gggctcgggg gaggcccagg ccggctgcca
      721 gtgacgctgt ccgctgagcg cgccgcgcaa cagcgcgagg gccgagtagc tcaccgcccg
      781 cgcttcaggc cttgtcgcgg tacaccgtct ggatcgggcg gattctcttg tcggtcccga
      841 agatcccgtg cttggacatg tcgacggtgt agcggctcgc cggcggacgg cgcccgaggc
      901 tctcggcgag cgcgcggatg tggtgcggtc ccgcgccgca gcacacgccg agatagcgca
      961 caccgagcgc gaacgcctcg cggccgaact cgaagatctc ggagcgcgtc gccatgaagg
     1021 ggtcgagacc cacggggaag gcgcgctgat tgttgcagca cgggtcggtg agcgacatga
     1081 acgagggttg ctgcgaggtc gtgcggtacg gcaccggcag tgccgccacg tggcacttca
     1141 ccgcctgccg gatgtcacgg atcatcggca tcatggtcgc aggtccccgg tggcagttga
     1201 gtccgaccac gtccgcccct gcctgctcca tgcgccggca ggcctccgcc ggcgtgagtc
     1261 cgtcgcgcag gatcggctcc tggtgcatcg caaaggtgat gaccgtcggc actttcgcct
     1321 gccgcttgat ggccttgagc gcgagctccg cctcgcccac ccaggcgagc gtctccgcca
     1381 cgaaataatc gaccccggcc tcgaccgccc agcctacctg ctcgtcgaag atccgctcga
     1441 cctccttgat ggcgcgggcg tcgttcggat cgtagatatt ggtgttgcac aggtccccgg
     1501 cgaagagcgt cccggtcctg cgcgccaccg acttcgcgat cctgagcgcc gcgcgattca
     1561 tcggtgccag gtccttctca cggccgatga tgcgcagttt ctcgcggtgc acgtagtagg
     1621 tgagcgcctg cgtcacgtcc gagcccgcat gcacgaaatc caggtgcagc tgctcgacga
     1681 cctcagggtg ctcgaacaga acctcgggca cgaaggcccc ggcctgcaga tagccgcgcc
     1741 gctccagctc gaacacatag ccctcggcgc agatcaccgc cccctcccgc aggcgcgcca
     1801 ggagatcccg ctccttcccg ttacccttgc cgacgcgctg gacggctcgc gctgctgatt
     1861 tcgctgtcgc gctcttcgcc ttggcggact tcaccttcga tctggccctg gct
//
LOCUS       DATLKL010000321         2432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_150375, whole genome shotgun sequence.
ACCESSION   DATLKL010000321 DATLKL010000000
VERSION     DATLKL010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150375"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VM713_04205"
     CDS             <1..752
                     /locus_tag="VM713_04205"
                     /inference="COORDINATES: protein motif:HMM:NF036968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HVH83503.1"
                     /translation="DPDSPWLARLLPLANELVAFPGFPRHLSQHVGGFVISEGALEEL
                     VPIENAAMPERTVVQWDKDDLNDLGLLKVDLLALGMLTALKRAFALVNDYRGTHHALG
                     ELPAEDPRVYDMICRADTIGVFQIESRAQMAMLPRLKPRNYYDLVIEVAIVRPGPIQG
                     DMVHPYLRRRCGTEPIEYPGEEVRAVLHRTLGVPLFQEQVMQIAIVAAGFSPGEADAL
                     RRAMGAWKRTGGLDAFRERLLTGMRDKGYPD"
     gene            777..1760
                     /locus_tag="VM713_04210"
     CDS             777..1760
                     /locus_tag="VM713_04210"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HVH83504.1"
                     /translation="MEGFGDYGFPEAHALSFALLVYDSSWLKCYEPAAFTCALLNSQP
                     MGFYAPAQLVRDARAHGVEVRPVDACVSLWDSTLERCADGEPALRLGLRLVKSLTQAA
                     AQRLIEARIVRPFDSVPELAARAALDRGDLEALAAAGAFASLSGNRHLAFWEVAGSER
                     PLPLATAADPQAGEGAGFMEGRPLLEKPSEGEEICADYAAVGLTLGRHPLALLRARLS
                     ADSLLSASELGRAANGAKVRTAGIVLMRQRPSTASGVTFVTLEDESGQVNVIVWERVG
                     QAQRHALIESQLLEVHGELQHQDGVMHVIARRLIDRTALLGQLTTRSRDFH"
     gene            2138..>2432
                     /locus_tag="VM713_04215"
     CDS             2138..>2432
                     /locus_tag="VM713_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83505.1"
                     /translation="MPHVYIVQARSAAEAGSAVRQAGGEVRGELTVIRAVSALLDARE
                     LAALRRASIPFLNVYDDTQVHATSLGTLPETYYPSEIAARDLQVGGITGTGVTV"
BASE COUNT          368 a          808 c          848 g          408 t
ORIGIN      
        1 tcgatcctga cagtccctgg ctcgcgcgac tgctcccgct cgcgaacgag ctcgtggcct
       61 ttcccggctt tccgcgccac ctgtcgcagc acgtcggcgg cttcgtgatc tccgaggggg
      121 cgctcgagga gctggtgccg atcgagaacg ccgccatgcc cgagcgcacc gtggtgcagt
      181 gggacaagga cgacctcaac gacttaggcc tcctcaaggt ggatctgctg gcgctcggca
      241 tgctcaccgc gctgaagcgc gcttttgcgc tggtgaacga ctaccgcggc acgcaccacg
      301 cgctcggcga gctgccggcc gaggacccgc gcgtctacga catgatctgc cgcgccgaca
      361 ccatcggtgt gttccagatc gaatcgcgcg cgcagatggc gatgctgccg cgcctcaagc
      421 cgcgcaacta ctacgacctg gtcatcgaag tcgcgatcgt gcgtcccggg ccgatacagg
      481 gcgacatggt gcatccctac ctgcggcgcc gctgtgggac ggagccgatc gagtatccgg
      541 gcgaggaagt gcgcgcggtg ctgcatcgca cgctcggcgt gccgctcttt caggagcagg
      601 tgatgcagat cgcgatcgtc gccgcgggct tctcgcccgg agaagcggat gcgctgcgcc
      661 gggcgatggg cgcgtggaag aggaccggcg ggctggatgc ctttcgcgaa cgcttgctga
      721 cggggatgcg tgacaagggc tatccggatt agttcgcgga gcgcatctat aggcaaatgg
      781 agggtttcgg cgactatgga tttccggaag cgcatgccct gagcttcgcg ctgctggttt
      841 acgattcgtc ctggctcaag tgctatgagc cggcggcctt cacttgcgcg ctgctcaaca
      901 gccagccgat gggcttctat gcgccggcgc agctggtgcg cgatgcgcgt gcccatggcg
      961 tcgaggtgcg tcccgtggat gcctgtgtct cgctctggga ctcgacgctc gagcggtgtg
     1021 ccgacggcga gccagcgctg cgcctggggc tgcggctggt gaagtcgctg acgcaagcgg
     1081 ccgcgcagcg tctgatcgag gcgcgcatcg tacgcccctt cgacagcgtg ccggagctgg
     1141 ccgcgcgcgc agcgctcgac cgcggcgacc tggaagcgct cgccgcggcc ggagcattcg
     1201 cttccttgag tggcaatcgg catctggcct tctgggaggt cgccggcagc gagcggccgc
     1261 tgccgctcgc caccgctgca gacccgcagg cgggcgaggg cgcaggcttc atggagggcc
     1321 gcccgctgct cgagaagccc tccgagggcg aggagatctg tgccgactac gccgccgtgg
     1381 ggctcacgct cggccggcat ccgctcgcgc tgctgcgcgc gcggctgtct gccgactcac
     1441 tcctgagtgc gagcgagctc ggccgcgccg ccaatggcgc caaggtgcgc accgccggca
     1501 tcgtgctcat gcgccagcgc ccgtccaccg cgagcggcgt taccttcgtc acgctcgagg
     1561 acgagagcgg ccaggtgaat gtcatcgtgt gggagcgcgt ggggcaggcg cagcgccacg
     1621 ccctcatcga atcgcagttg ctcgaggtgc atggcgagct gcagcaccag gacggggtca
     1681 tgcacgtgat cgcccggcgg ctcatcgacc gcacggcgct gctcggacag ctcacgaccc
     1741 gctcgcggga ttttcactga cgccgcaggc gcagcatatc gcgcgcgcgt ggtgcccgtg
     1801 ccacggccag cgctgttatg ttgatccatc gcaacgctat gttgagtacg cgcgcactgc
     1861 tgcgctggac ataacagcga cacacgaatc ctgagaagtg cgtcgcactc cgcagtggac
     1921 cgtgtcactg tccggcgaca cccgttgtag taaaaatgga gccgtagaaa aaagggccgc
     1981 tccaacacca aaggtgggtt cgatgaaggt gcaccgcgag ccatgggtac cggctgtcgc
     2041 cggcgtcatt tccgcaatat tctccctttt ggttttgtcg ggcggtgacg ccccgcgctc
     2101 aggtgcgcaa gcgcccgcgc ctagcccggc gagcggcgtg ccgcatgtct acatcgtgca
     2161 ggcgcgcagc gccgctgagg cgggcagcgc ggtccggcag gccggcggcg aggtccgcgg
     2221 cgagctgacc gtcatccgcg ccgtcagcgc gctgctcgat gcgcgcgagc tggccgcgct
     2281 gcgccgggct tcaatcccgt tcctcaacgt gtatgacgac acgcaggtgc atgcgacttc
     2341 attgggcacg ctgcccgaga cctactaccc gagcgagatc gccgcgcggg acttgcaggt
     2401 cgggggcatt accggcaccg gagtgacggt tg
//
LOCUS       DATLKL010000322         1839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_127765, whole genome shotgun sequence.
ACCESSION   DATLKL010000322 DATLKL010000000
VERSION     DATLKL010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127765"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VM713_04220"
     CDS             complement(<1..1314)
                     /locus_tag="VM713_04220"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HVH83506.1"
                     /translation="MVSEIRQFFRDHGISEVEAIIPDMAGIARGKIMPAEKFAEDGGM
                     RLPESVFLQTVTGDYPPETGDAMSPAEIDIVLKADPKTVRRVPWAAEPTAQVIHDCFY
                     SDGRRVSMAPRHVLRNILELYTQRGWEPVVAPELEFYLVEQNKDADYPLKPPVGRSGR
                     AEPGRQSYSIAAVNEFDPLFDDIYQFCEDQDIEIDTLIHEDGPAQMEINLIHGQPMDL
                     ADQVFPFKRTAREAALRHKMYATFMAKPHAKEPGSAMHIHQSVVDVKTRKNIFSNPDG
                     TPSPLFFSHIGGLQRYMPAAMALFCANVNSYRRLTRYLSSPINVHWGYDNRTAGLRVP
                     MSDPQARRVEDRVGGADANPYIAIAASLACGYLGMVEGVQPSDPIVGSAHDLPFGLPR
                     SLDEALRELRACEPLVKLLGEPFVEAFTIVKEAEYEVFLQVISSWE"
     gene            complement(1326..>1839)
                     /locus_tag="VM713_04225"
     CDS             complement(1326..>1839)
                     /locus_tag="VM713_04225"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HVH83507.1"
                     /translation="SAPGTLHDPARDATTLPLIRHAVDSGVPVLGICRGFQEMNVAFG
                     GTLHQRLHEVPGLLDHRDDESQPLEVQYGPAHDVTLEPGGVLRSLADGDRIRVNSLHS
                     QGIERLGAPLAVEARAPDGVIEAFRVRAAASFALAVQWHPEWQVMTNPFSRVLFAAFG
                     KAAAERNRQK"
BASE COUNT          279 a          608 c          643 g          309 t
ORIGIN      
        1 ctcccaggag ctgatcacct gcaggaacac ctcgtactcg gcctccttga cgatcgtgaa
       61 cgcctcgacg aaaggctccc cgaggagctt caccagcggt tcgcaggcgc gcagctcgcg
      121 cagcgcctca tcgagcgagc gcggcaggcc gaacggcaga tcgtgcgcac tgccgacgat
      181 cgggtcggag ggctgcacgc cctcgaccat gcccagatag ccgcaggcga gcgaggcggc
      241 gattgcgatg taggggttgg cgtcggcgcc gccgacgcgg tcctcgacgc ggcgcgcctg
      301 cgggtccgac atcggcacgc gcagtcccgc ggtgcggttg tcgtagcccc agtggacatt
      361 gatgggcgag gacaggtagc gcgtgagtcg ccggtaggag tttacgttgg cgcagaacag
      421 cgccatcgcg gccggcatgt aacgctgcag cccgccgata tggctgaaga acagcggcga
      481 cggcgtaccg tcggggttgg agaagatgtt cttgcgcgtc ttcacgtcca ccacgctctg
      541 gtggatgtgc atggcgctgc cgggctcctt ggcgtgaggc ttggccatga aggtggcgta
      601 catcttgtgg cgcagcgccg cctcgcgcgc cgtgcgcttg aagggaaaca cctggtcggc
      661 gagatccatc ggttggccgt ggatcaggtt gatctccatc tgcgccgggc cgtcctcatg
      721 gatcagggta tcgatctcga tgtcctggtc ctcacagaac tggtagatgt cgtcgaacag
      781 cggatcgaac tcgttgaccg cggcgatgct gtaggactgg cggccgggct cggcgcgccc
      841 ggagcgcccg accggcggct tgagcggata gtccgcatcc ttgttctgct ccaccagata
      901 gaactcgagc tccggcgcca ccaccggctc ccagccgcgc tgcgtgtaga gctcgaggat
      961 gttgcgcagc acgtggcgcg gtgccatcga cacccggcgg ccgtccgaat agaagcagtc
     1021 gtggatcacc tgcgcggtgg gctccgccgc ccacggcacg cggcgcaccg tcttgggatc
     1081 ggccttgagg acgatgtcga tctccgccgg gctcatggca tccccggtct ccggcgggta
     1141 gtcgccggtt acggtctgca ggaacacgct ctcgggcagg cgcatgccgc cgtcctcggc
     1201 gaacttctcc gcgggcatga tcttgccgcg ggcgatgccg gccatgtcgg ggatgatcgc
     1261 ctcgacctcc gagatgccgt gatcgcggaa gaattgccgg atctcactca ccatgggcgc
     1321 gcctttcact tctgcctgtt gcgctcggcc gccgccttgc cgaaggcggc gaacagtacg
     1381 cgcgaaaacg ggtttgtcat gacctgccac tccgggtgcc attgcacggc gagcgcgaag
     1441 ctcgctgccg cgcgtacccg gaatgcctcg atgacgccat ccggagcccg cgcctcgacc
     1501 gcgagcggcg caccgaggcg ctcgatgccc tggctgtgga gcgagttcac ccggatgcgg
     1561 tcaccgtcgg cgagggagcg cagcacgccg ccgggctcga gggtcacgtc gtgcgccgga
     1621 ccgtattgca cctcgagcgg ctggctctcg tcgtcgcggt gatcgaggag ccccggcacc
     1681 tcgtgcagcc tctggtgcag cgtcccgccg aaggcgacgt tcatctcctg aaagccgcgg
     1741 cagattccga gcaccggcac gcccgagtcg acggcgtggc ggatcaatgg cagcgtcgtc
     1801 gcatcgcgcg cggggtcgtg cagggttccg ggggcgctc
//
LOCUS       DATLKL010000323         2055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_116525, whole genome shotgun sequence.
ACCESSION   DATLKL010000323 DATLKL010000000
VERSION     DATLKL010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116525"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1893
                     /gene="glgB"
                     /locus_tag="VM713_04230"
     CDS             <1..1893
                     /gene="glgB"
                     /locus_tag="VM713_04230"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HVH83508.1"
                     /translation="PVMFLTEHDIYHFREGSYFRAYERLGAHAAGADPAHATHFALWA
                     PNASAVSVVGDFNSWQDGRHPLHPRHDSSGIWEGTVEGIGPGTLYKYHVVGRDGHTAT
                     DKADPYAFRAEVPPRTASVVWDLGYEWRDADWMRARAGANALGAPWSIYEVHLGSWRR
                     VPEEANRSLGYRELAPALADYVAGLGFTHVELLPVMEHPFYGSWGYQVTGYFAPSARY
                     GTPQDFMYLVDYLHQRGIGVVLDWVPSHFPTDEHGLARFDGTHLYEHADPRQGFHPEW
                     NSSIFNYGRAEVRNFLASSALFWLEKYHIDALRVDAVASMLYLDYARRAGEWIPNSFG
                     GHENLEAVEFLRQLNLAVYRDHPETQTIAEESTAWPMVSRPTYLGGLGFGLKWNMGWM
                     HDTLEYFQQDPVHRKYHHSRLTFSLWYAFTENFVLPLSHDEVVHGKGSLIGKMPGDEW
                     QQFASLRLLFGYMWTHPGKKLLFMGGEFGQRREWQHEESLEWHVLQYPLHAGVQRWVR
                     DLNRLYRDTPALYELDFSEAGFSWIDCDDADVSVISFLRRDRSGGVALVACNFTPVPR
                     ERYRIGVPHGGRWRERLNSDAADYGGSGQGNLGALEAAQIPAHGHGFSLHLRLPPLAV
                     VVLTPG"
BASE COUNT          319 a          719 c          682 g          335 t
ORIGIN      
        1 ccggtgatgt tcctcaccga gcacgatatc tatcacttcc gcgaaggctc ttacttccgt
       61 gcctatgagc ggcttggcgc gcacgccgcc ggcgccgacc cggcacacgc gacccacttc
      121 gcactgtggg cgcccaacgc gtccgcggtc tcggtggtgg gggatttcaa cagctggcag
      181 gacggccgcc atccgctgca tccgcgccac gactcctccg ggatctggga gggtaccgtg
      241 gagggcatcg gccccggcac cctttacaag taccacgtgg tcgggcgcga cggccatacg
      301 gccaccgaca aggccgatcc gtatgctttc cgcgccgagg tcccgccgcg caccgcctcg
      361 gtagtctggg atctcggcta cgagtggcgc gacgcggact ggatgcgcgc acgcgcgggc
      421 gccaacgcgc tcggcgctcc ctggtcgatc tacgaggtgc acctcggatc gtggcggcgc
      481 gtccccgagg aagctaaccg ctcgctcggc taccgtgagc tcgcacccgc gctcgccgac
      541 tatgtcgccg ggctcggctt cacgcacgtc gagctgttgc cggtgatgga gcatcctttc
      601 tacggctcgt ggggctacca ggtgaccggt tatttcgccc cgagcgcgcg ctacggcacg
      661 ccacaggact tcatgtacct cgtcgactat ctgcaccagc gcggcatcgg cgtggtgctc
      721 gactgggtgc catcgcactt tccgaccgac gagcacgggc tcgcgcgctt cgacggcacg
      781 cacctgtacg agcacgccga cccgcgccag ggctttcacc cggagtggaa cagctcgatc
      841 ttcaactacg gtcgcgccga ggtgcgcaac ttcctggcga gcagcgcgct gttctggctc
      901 gagaagtatc acatcgacgc gctgcgcgtg gacgccgtcg cctcgatgct ctatctcgac
      961 tacgcacggc gcgccggcga gtggatcccc aacagtttcg gcgggcacga gaatctcgag
     1021 gccgtcgagt tcctgcgtca gctcaacctc gccgtgtacc gcgaccaccc cgagacgcag
     1081 acgatcgccg aggaatcgac cgcctggcca atggtgtcgc gtccgaccta cctcgggggg
     1141 ctggggttcg gcctcaagtg gaacatgggc tggatgcacg acacgctcga gtacttccag
     1201 caggatccgg tgcaccgcaa gtaccaccac agccgcctca ccttcagcct ctggtacgcc
     1261 ttcaccgaga acttcgtgct gccgctctcg cacgatgagg tggtgcacgg caagggctcg
     1321 ctcatcggca agatgcccgg cgacgagtgg caacagttcg cgagcctgcg tctgctgttc
     1381 ggctacatgt ggacgcatcc gggcaagaag ctgctgttca tgggcgggga attcgggcag
     1441 cggcgcgagt ggcagcacga ggagagtctc gagtggcacg tgctgcagta tccgctgcac
     1501 gcgggcgtgc agcgctgggt gcgggacctc aaccgcctgt accgcgacac gcccgccctc
     1561 tacgagctcg acttcagcga ggcgggcttc agctggatcg actgcgacga cgccgacgtg
     1621 agcgtgatct cgttcctgcg ccgcgatcgg tccggagggg tcgcgctcgt cgcctgcaac
     1681 ttcacgccgg tgccgcgcga gcgctaccgg atcggcgtgc cgcacggcgg gcgctggcgg
     1741 gagcgcttga acagcgacgc cgccgactac ggcggcagcg gccaggggaa tctgggcgca
     1801 ctcgaggccg cacagatccc agcgcacggc cacggcttct cgctgcacct gcgactgccg
     1861 ccgctggccg tggtggtact cacgcccggc tagggcgcgc ccgtaaacgc acgccgcccc
     1921 tcgtgcgttg actcgactgg cgggacactg gaggtcggat cagaccatgc ggcacaagct
     1981 cacggcactc acggcagtgg ccgcactggc agcgctgctg gccggctgcg ccacgggcgg
     2041 cgcgtcagcc ggttc
//
LOCUS       DATLKL010000324         3712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_127798, whole genome shotgun sequence.
ACCESSION   DATLKL010000324 DATLKL010000000
VERSION     DATLKL010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127798"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1835)
                     /gene="sdhA"
                     /locus_tag="VM713_04235"
     CDS             complement(51..1835)
                     /gene="sdhA"
                     /locus_tag="VM713_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HVH83509.1"
                     /translation="MNAYPFIDHTYGVVVVGAGGAGLRATLGLAESGLSTACITKLFP
                     TRSHTVAAQGGISAALGNMGEDDWRWHMYDTVKGSDWLGDQDAIELMCKEAPAAVIEL
                     EHYGVPFSRTETGRIYQRPFGGMTTHFGKGIAQRTCAAADRTGHAMLHTLYQQSLRHA
                     ATFFVEYFAIDLIMQDGACRGVLALDLAEGTLHRFRSHVTILATGGYGRTYFSCTSAH
                     SCTGDGNAMVLRAGLPLQDMEFVQFHPTGIYGAGCLITEGARGEGGYLTNSKGERFMG
                     RYAPNAKDLASRDVVSRAMTLEIREGRGAGPEHDYIELHLEHLGADVIHERLPGIAET
                     ARIFAGVDVTREPIPVQPTVHYNMGGIPCNHRAEVVCPKSGDPDCVVPGLMAVGEAAC
                     VSVHGANRLGSNSLLDLVVFGREAARRCVEIVEPGSSHRPLAADAGEAAVTRLDRLRR
                     AAGARGTAAIRVEMQKTMQLDAAVFRTGESLAQGIARIGQTFESFADVRITDRSLIWN
                     TDLVETLELENLLLQSTATIHSAANRTESRGAHAREDFPQRDDRNWMKHTLVWVEGPK
                     QVRFDYRPVHLNTLTAEVESIPPRARTY"
     gene            complement(1832..2218)
                     /gene="sdhD"
                     /locus_tag="VM713_04240"
     CDS             complement(1832..2218)
                     /gene="sdhD"
                     /locus_tag="VM713_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HVH83510.1"
                     /translation="MSLRTPLRRVLGAGSAKEGVQHWWQQRLTAVALVPLSVWFVVSL
                     LALPSLDHATVRAWMGQSWTALLLILLVLVAAWHSQLGLRVIVEDYVPAPGARTVTLV
                     LLSFLHTLLAAAGVFAVLRVALGSGA"
     gene            complement(2215..2610)
                     /gene="sdhC"
                     /locus_tag="VM713_04245"
     CDS             complement(2215..2610)
                     /gene="sdhC"
                     /locus_tag="VM713_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HVH83511.1"
                     /translation="MPARPLSPHLSVYKMSRYTLATSIFNRFTGVGLSVGLVLLVYWL
                     MSVAGGPGSQARAARLLSLPVVKLVYAALVVAFCYHLVAGIRHLVWDTGRGLERAQSQ
                     KSAWLVVAVSMALSLLIGYCVLVLKGHVP"
     gene            2845..>3712
                     /locus_tag="VM713_04250"
     CDS             2845..>3712
                     /locus_tag="VM713_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83512.1"
                     /translation="MTATESQGKTTHNSMYAQWNLLPLESLGALRARGRDVIPFLQGQ
                     LSNDLTGLAAERSLLAGYHNPQGRVLALMRLIEPAPGEALAVMPRELVTPTLSRLRTF
                     VLRSKVELTDESLAWRLEGLIGEGGSEPAAGAAPSAPPFAARLPGGVGAVARLGDSFA
                     LRVGERPARWLIISPAAAPLGDPALAGCAPAVPQLWQRFGIENGEPQVYTATSGEFVS
                     QMLNLDVLGAIAFDKGCYTGQEVIARAHYRGRVKRRLQRFRTRELRPLAPGDAGELAD
                     GRTFRVVDAVRLA"
BASE COUNT          604 a         1269 c         1304 g          535 t
ORIGIN      
        1 accgggcgcg agttaggggg caggcggaac tcagccatgc tcgaggtcct tcagtaagtg
       61 cgcgcccggg gcggaatcga ctccacctcc gcggtgagcg tattgagatg caccgggcgg
      121 tagtcgaagc gcacctgctt cggtccctcg acccacacca gcgtgtgctt catccagttg
      181 cggtcgtcgc gctgcgggaa atcctcgcgc gcatgcgcgc cgcggctctc ggtgcggttg
      241 gccgccgaat gaatcgtcgc ggtcgactgc agcagcagat tctcgagctc gagcgtctcg
      301 acgagatccg tgttccagat gagcgagcga tcggtgatgc gcacgtcggc gaaggactcg
      361 aaggtctggc cgatgcgcgc gatcccctgg gcgaggctct cgccggtgcg gaacaccgcg
      421 gcatcaagct gcatggtctt ctgcatctcg acgcggatcg ccgcggtgcc gcgggcgccc
      481 gcggcacgcc gcagccggtc gagacgcgtc accgccgcct caccggcgtc cgcggcgagc
      541 ggccgatgcg agctgccggg ctcgacgatc tccacgcagc ggcgtgccgc ctcgcggccg
      601 aagaccacca ggtcgagaag cgaattggaa cccaagcggt tggcgccgtg caccgacacg
      661 caggcggcct cgccgacggc catgaggccg gggacgacgc agtcgggatc cccgctcttc
      721 ggacacacca cctcggcgcg atgattgcag gggatgccgc ccatgttgta gtgcaccgtc
      781 ggctgcaccg ggatcggctc gcgcgtcacg tcgacgccag cgaagatacg cgccgtctcg
      841 gcgatgcccg gcagccgctc gtggatcacg tcagcgccga gatgctccag atgcagctcg
      901 atgtagtcgt gctcggggcc ggcgccgcgg ccctcgcgga tctcgagcgt catcgcgcgg
      961 ctcaccacat cgcgggaggc gagatccttg gcgttcggcg cgtagcgtcc catgaagcgc
     1021 tcgcccttgg agttcgtgag gtagcccccc tcaccgcgcg ctccctcggt gatcaggcag
     1081 ccggcgccgt agatcccggt gggatggaac tgcacgaact ccatgtcctg cagcggcaac
     1141 cccgcccgca gcaccatggc gttgccgtcc ccggtgcacg agtgggccga cgtgcacgag
     1201 aagtaagtgc ggccgtagcc accggtcgcg aggatggtga cgtgcgaacg gaaacgatgg
     1261 agcgtcccct cagcgagatc aagcgccagg acgccgcggc acgcgccatc ctgcatgatg
     1321 aggtcgatgg cgaagtactc gacgaagaag gtcgcggcgt ggcggagcga ctgctggtaa
     1381 agagtgtgga gcatggcgtg cccggtgcgg tcggcggccg cacaggtgcg ctgggcgatg
     1441 cccttcccga agtgcgtggt catgccgccg aaagggcgct ggtagatacg cccggtctcg
     1501 gtgcgcgaga acggcacgcc gtagtgctcg agctcaatca ccgccgccgg cgcctccttg
     1561 cacatgagtt cgatcgcatc ctggtcgccg agccagtccg agcccttgac ggtgtcgtac
     1621 atgtgccagc gccagtcgtc ctcgcccatg ttgccgaggg cggccgagat gccgccttgg
     1681 gccgctaccg tgtggctgcg cgtcgggaag agcttggtga tgcaggcggt cgagagtccg
     1741 ctctcggcga gcccgagcgt ggcacgcagg cccgcgccgc cggcgccgac caccacgacg
     1801 ccataggtgt gatcgatgaa cgggtaggcg ttcacgctcc gctcccgagc gccaccctga
     1861 gaaccgcgaa caccccggcc gccgccagca gcgtgtgcag gaaggagagc agcacgaggg
     1921 tcaccgtgcg ggcccccggg gctggcacat agtcctcgac gatcacccgc aggccaagct
     1981 gcgagtgcca ggccgccacc agcacgagaa gaatgaggag cagcgccgtc cagctctggc
     2041 ccatccaggc ccgcacggtg gcgtgatcca acgacggcag cgccaggagc gataccacga
     2101 accagacgct caagggtacc agcgccacgg cggtgagccg ttgctgccac cagtgctgca
     2161 cgccctcctt cgccgagccc gcgccaagga cgcgccgcaa cggggtgcgc aggctcacgg
     2221 cacgtggccc ttcagcacga ggacgcagta accgataagg agtgagagcg ccatgctgac
     2281 cgcgacgacg agccatgcgc tcttctgtga ctgtgcgcgc tcgagcccgc ggcccgtgtc
     2341 ccatacgagg tggcggatgc cggccaccag gtggtagcag aaggcgacga ccagggcggc
     2401 gtagacgagc ttcacgaccg gcagcgacag cagccgcgcg gcgcgcgctt gactccccgg
     2461 cccgcccgcc acgctcatca gccagtacac gagcagcacg aggccgaccg acagcccgac
     2521 gcccgtaaag cggttgaaga tcgaggtcgc cagggtgtac ctggacatct tgtaaaccga
     2581 caggtgcggg gagagcggcc gggcgggcat gggctaaaaa tcgatgcagc gaccgttctt
     2641 ctcccaatcc ccgaagcgcg tcgggtcggg cccgggcgtg ccgccgatct ctcgcgaccg
     2701 ccgcggcgcg ggcgcaggat ccaggggaac gagagggccg acgcgatgct cagactggag
     2761 aggaacggaa ggggcggacg gaggccgtcc gggcctcttg cgttgcgacg tcgacatact
     2821 tattgagtct gccagaattc cctcatgacg gccacggaaa gccaaggaaa aacaacgcac
     2881 aactccatgt acgcacaatg gaatctgctg ccgctcgagt ctctcggcgc gctgcgggcg
     2941 cgtggccgcg acgttattcc gtttctgcag gggcagctct cgaacgactt aacagggctc
     3001 gccgccgagc gctcgctgct tgccggctac cacaacccgc agggccgcgt gctcgcgctg
     3061 atgcgcctca tcgagcccgc gcccggcgag gcgctcgccg tgatgccgcg cgagctggtg
     3121 acgccgacgc tcagccggct gcggactttc gtgctgcgct cgaaagtcga gctcacggac
     3181 gaatcgctcg cctggcgcct cgaggggctc atcggggaag gcgggtcgga gcctgcagcg
     3241 ggcgccgcgc cgtccgcccc gccctttgct gcgcggctgc cgggcggcgt gggcgcagtc
     3301 gcgcgtctcg gggactcctt tgcgttgcgt gtcggcgagc gaccggcgcg ctggctgatc
     3361 atctccccgg cggcagcccc gctcggcgat cccgcgctcg ccggctgcgc gcccgcggtg
     3421 ccgcagctgt ggcagcgctt cggtatagag aacggcgagc cgcaggtcta cacggcgacc
     3481 tcgggagaat tcgtctcgca gatgctgaac ctcgacgtgc tcggcgcgat cgccttcgac
     3541 aagggctgct acaccggcca ggaggtgatc gcgcgtgccc actaccgcgg cagggtcaag
     3601 cgccgtctgc agcgctttcg gacgcgcgag ctgcggccgc tcgcgccggg cgatgccggc
     3661 gagctcgccg acgggcgcac gttccgtgtc gtggacgcgg tgcgacttgc gg
//
LOCUS       DATLKL010000325         1592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_37571, whole genome shotgun sequence.
ACCESSION   DATLKL010000325 DATLKL010000000
VERSION     DATLKL010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37571"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="VM713_04255"
     CDS             <1..1313
                     /locus_tag="VM713_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVH83513.1"
                     /translation="SLSEFCAWCVCLGGWAMLLFAVPTAHVLLPLLTLSILLVVWAAA
                     RLGAALASLAALVFAMLAAGSFALQLGALATPDAATGVAYVWGFVGVLSVISLFLAAL
                     LAEHDGRRREISAVNQRYRRLFQGDPRPLWLHDTRSGQILEANEPAARAYGYSMAEFT
                     ALQVTQLLAPGMSPAALAAPGDRSVGPFAMRHLRKSGDSLEVEMWSYGTFLDGRRVSI
                     CFAHDVTERNTLRRLLFDRAELERRELAAELRRTLAGPLAELRIVAHKLLLELGRRAA
                     SPRTRELLESLARQARRAAERCREVAQRLSPLQANCGDLVAALEALRRQTPEGAPLEI
                     SVIGEVPLALDQQQSEHLYSLLSEILTRCRSSRQGSVHVALRSFGHTVRVAVDADLPP
                     EPAGAPPSLARHPSVLLRVRAMGARLWERSSGGAYTRLVCDYPL"
     gene            complement(1342..>1592)
                     /locus_tag="VM713_04260"
     CDS             complement(1342..>1592)
                     /locus_tag="VM713_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83514.1"
                     /translation="DQLHIGALRGDLSCGLDAVQLGHGDDENCNARRKLQDLRHGIAA
                     VAGGSNYPDIGLRSEVCDQAIAYDGVVIGDEDGNCCHG"
BASE COUNT          221 a          559 c          546 g          266 t
ORIGIN      
        1 aaagcctgtc ggagttctgc gcctggtgcg tctgcctcgg cgggtgggcg atgctgctgt
       61 tcgctgttcc caccgcgcac gtgttgttgc cgcttctcac cttgagcatc ctgctggtgg
      121 tgtgggccgc ggcccgcctc ggcgcggcgc tcgcctcgct cgctgccctg gtgttcgcca
      181 tgctggccgc cgggagcttt gccctgcagc ttggggcgct cgctacgccc gacgcagcga
      241 ccggggtggc gtacgtctgg ggcttcgtcg gagtgctcag cgtcatcagc ctctttctcg
      301 ccgcgctgct cgccgagcac gacggccggc gccgcgagat cagcgcagtc aaccagcgct
      361 atcgtcgcct gttccagggc gatccgcggc cgctatggct gcacgatacc cgcagcgggc
      421 aaattctcga ggcgaacgag cccgcggcgc gtgcctacgg ctactcgatg gcggaattca
      481 ctgcgctgca ggtgacgcag ctgctcgccc ccgggatgag ccctgcggca ctggccgctc
      541 ctggagaccg gtccgtgggg ccgttcgcga tgaggcatct gcggaaatcc ggcgacagcc
      601 tggaagtgga aatgtggtcg tacggcacct tcctcgacgg acgccgggtc agcatctgct
      661 tcgcccacga cgtgactgag cgcaatacgc tgcggcgtct gttgttcgac cgggccgagc
      721 tcgagcggcg cgagctcgcc gcggagctgc gccgcacgct ggccggcccg ctggccgagc
      781 tgcgcatcgt ggcccacaag ctgctgctgg agctcggacg gcgggcggcg tcccccagga
      841 cgcgggaatt gctcgaatct ctggcgcggc aggcgcggcg cgcagccgaa cgctgccgcg
      901 aggtcgcgca gcgactctca ccgctgcagg ccaactgcgg cgatctggtc gcggcgctcg
      961 aggcgctgcg gcgccagact ccggagggcg cgcccctcga gatcagtgtc atcggggagg
     1021 tgccgctggc gctcgaccaa cagcagtccg agcacctcta cagtctactc agcgagatcc
     1081 tcactcgctg ccgctcgagc cgccagggaa gcgttcatgt ggccttgcgg agcttcggcc
     1141 acaccgtgcg cgtggcggtc gacgcggatc tgccgccaga gcccgcgggg gcgccaccga
     1201 gcctcgcacg ccacccctcg gtcctcctgc gggtcagggc catgggcgcg cgcctctggg
     1261 agcgctcgag cggcggcgca tacacccggc tggtctgcga ctacccattg tgagacagcg
     1321 cggcaccgcg ggtgtaagca actacccatg acagcagtta ccgtcttcat cgccgatgac
     1381 cacgccatcg tacgcgatgg cctggtcgca cacctcgctg cgcagcccga tatcagggta
     1441 gttggaaccg ccagcgacgg ccgcgatgcc gtggcgcagg tcctgcaact tgcgccgcgc
     1501 gttgcaattc tcgtcatctc catgcccgag ctggacggca tcgaggccgc aagacagatc
     1561 gccgcggagt gccccgatgt gcagttgatc at
//
LOCUS       DATLKL010000326         1777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_172831, whole genome shotgun sequence.
ACCESSION   DATLKL010000326 DATLKL010000000
VERSION     DATLKL010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172831"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1777
                     /locus_tag="VM713_04265"
     CDS             <1..>1777
                     /locus_tag="VM713_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH83515.1"
                     /translation="VRTGQGDIVADHLVLATGNYARQTGRLLGLNVPAIPVEHQYIVY
                     DESPELVAYRKGGGRELAVLRESDRSYYLREERMGWILGPYERGAPARFADGVPDWFG
                     RSLFPGDLDRLVPHVEAATRRVPALENCGIKDIVNGPISYTPDGSPLIGPAWGLPNVW
                     LNEGHSFGITAAGGAGWQLAEWIVEGEPGIDMLAVDPRRFGAYTSKRYVVQKNEETYR
                     SVFTIHYPDEERPDGRPAKTSPVYDKLNARGAVWGQRYGWERATWFAPAGVERKDRWS
                     FRRTNYFEHVGNEARLMRERVGVIDLTPFTKHEVTGPGAEAWLDGLVANKVPAKVGRM
                     ALSHALTRRGGIRSEFTITRIAPQHFYVVSAGAAERYDSDYLFTSLPQDGSVALRNIT
                     NTRGTFVLAGPKSREVLAQLTATPLDNNSFPWLTAQVIEAALAVDVYALRVNFVGALG
                     WELHFPVEYAHGLFDALFEAGRPHDIGMVGMRAMESLRMEKSYRMWGSDLTPDYTPLE
                     AGLDRFVRMGKGSFIGREALEKQLAVGVPNRFVTLEVHGVSDADPLGNEPLFEPGGRM
                     IGRATSGYYGHCLRKSLAIGYARTDR"
BASE COUNT          283 a          626 c          592 g          276 t
ORIGIN      
        1 gtgcgcaccg gtcaaggcga catcgtcgcc gaccatctgg tgctcgcgac cggcaactac
       61 gcgcgccaga ccggccgtct gctcggcctc aatgtgccgg cgatcccggt cgagcatcag
      121 tacatcgtct acgacgagtc tccggagctc gtcgcctacc gcaagggcgg cgggcgcgag
      181 ctggcggtgc tgcgcgagtc cgacaggtcg tactacctgc gcgaggagcg catgggatgg
      241 attctcggtc cctacgagag gggcgcgccg gcgcgcttcg ccgacggcgt gcccgactgg
      301 ttcggacgga gcctgttccc cggcgatctc gatcggctcg tgccgcacgt cgaggccgcg
      361 acccgccgcg tgcccgcgct cgagaactgc ggcatcaagg acatcgtcaa cggaccgatc
      421 tcctacacgc cggatgggag cccgctcatc ggcccagcct ggggccttcc caacgtgtgg
      481 ctcaacgagg gacacagctt cggcatcacc gccgcgggcg gtgcgggctg gcagctcgcg
      541 gaatggatcg tcgagggcga gcccggcatc gatatgctcg cggtcgatcc gcgccgcttc
      601 ggcgcataca cctcgaagcg ttacgtggtg cagaagaacg aggagaccta ccgtagcgtc
      661 tttacgatcc actatcccga cgaggagcgg cccgacgggc gccccgccaa gaccagtccg
      721 gtctacgaca agctgaatgc ccggggcgcc gtctgggggc agcgctacgg ctgggagcgc
      781 gccacctggt tcgcgcccgc cggggttgag cgcaaggacc gctggagctt ccgccgcacg
      841 aattacttcg agcacgtcgg caacgaggcg cgtctcatgc gcgagcgcgt cggcgtcatc
      901 gatctcaccc ccttcaccaa gcacgaggtc acgggccccg gcgccgaagc ctggctcgac
      961 ggcctggtcg caaataaagt gccggcgaag gtcggtcgca tggcgctctc gcacgcgctc
     1021 acccgccgcg gcggcatccg ctccgaattc accatcaccc ggatcgcgcc ccagcacttc
     1081 tacgtggtga gcgccggcgc cgccgagcgc tacgacagcg actatctctt cacgtcactg
     1141 ccgcaggacg gctcggtggc gctccgtaac atcaccaaca cccgtggcac cttcgtgctc
     1201 gcgggaccga agtcgcgcga ggtgctcgcg cagctcaccg ccacgccgct cgacaataac
     1261 tccttcccgt ggctcaccgc gcaggtcatt gaggccgcgc tcgccgtgga tgtctacgcc
     1321 ttgcgcgtca acttcgtcgg cgccctaggc tgggagctgc acttccccgt cgagtacgcg
     1381 catggcctct tcgacgcgct gttcgaggcg ggcaggcctc atgacatcgg catggtcggc
     1441 atgcgcgcca tggagtcgct gcgcatggag aagtcctacc gcatgtgggg cagcgacctg
     1501 acgcccgact acacgccgct cgaggcgggc ctcgatcgct tcgtgcgcat gggcaagggt
     1561 tcgttcatcg gccgcgaggc gctcgagaag cagctggcag tgggcgtacc caaccgcttc
     1621 gtgacgctcg aggtgcacgg ggtcagcgat gccgatccgc tcggcaacga gccgctgttc
     1681 gagcccggcg gccgcatgat cgggcgagcg acctcgggct actacgggca ctgcctgcgg
     1741 aagagcctgg cgatcggcta cgccaggacc gatcgct
//
LOCUS       DATLKL010000327         1639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_116565, whole genome shotgun sequence.
ACCESSION   DATLKL010000327 DATLKL010000000
VERSION     DATLKL010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116565"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VM713_04270"
     CDS             complement(<1..1101)
                     /locus_tag="VM713_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HVH83516.1"
                     /translation="MRSFLASLQRVGGALMLPIAVLPIAGLLLRLGQPDLLNSASIAA
                     AGGAIFDNLGLLFAAGVAVGLARENHGAAGLAAVVAYLVATKGAEVLISVPGSAVADL
                     AGRARDLGVAAYKAKELAKLSVPVGILSGLIAGALYNRFFNITLPSYLSFFGGRRFVP
                     IVSGLAGLALAAVFGMEWQPLERGMDALSESVLHAGAFGLFAYGLLNRILIVTGLHHI
                     INNIAWFLLGDYHGTTGDLKRFFAGDPSAGVFMSGFFPVMMFGLPAACLAMYHTALPA
                     RRAAVAGLLGSIALTSFLTGVTEPIEFTFIFLAPLLYAVHALLTGVAFIIMSALGVRL
                     GFSFSAGFIDYFLNFNHATRPLLLLPVGALYFA"
     gene            complement(1098..>1639)
                     /locus_tag="VM713_04275"
     CDS             complement(1098..>1639)
                     /locus_tag="VM713_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HVH83517.1"
                     /translation="TEAAEVQAVRAVLEELRAELGVTQAIEVGVMIETPASAVLADRL
                     LEVADFISIGTNDLTQYTLAMDRGHPQLAPRLDALHPAVLRLIETAAQAAATRGRMVA
                     VCGGLASEPLAAPLLVGLGVHELSAVGAVIPQLKANLSRVTVAECRALARSALAAADA
                     AAVRMLARRALGEGREAAQ"
BASE COUNT          302 a          559 c          585 g          193 t
ORIGIN      
        1 cgcgaagtag agcgcgccca ccggcagcag cagcagcggc cgcgtcgcgt ggttgaaatt
       61 cagaaagtag tcaatgaacc cggcggagaa gctgaagccg aggcgcacgc cgagcgcgct
      121 catgatgatg aaggcgaccc cggtgaggag ggcgtgcacg gcatagagga gcggcgcgag
      181 gaagatgaag gtgaactcga tcggctcggt gacgcccgtc aggaacgagg tgagcgcaat
      241 cgagccgagt agccccgcca cggcggcgcg ccgcgccggt aacgccgtgt ggtacatggc
      301 gaggcaggcc gccgggagtc cgaacatcat gaccggaaag aagcccgaca tgaagacgcc
      361 ggcgctcgga tcgccggcga aaaagcgctt caggtcgccg gtcgtgccgt gatagtcccc
      421 gagcaggaac caggcgatgt tgttgatgat gtgatggagc ccggtgacga tcagtatgcg
      481 gttcaggagg ccgtaggcga acaggccgaa ggctccggcg tgcagcaccg actcgctgag
      541 cgcatccatg ccgcgctcga gcggctgcca ctccatgccg aacacggccg cgagagccag
      601 ccccgccagc ccgctcacga tcggcacgaa gcgccgcccg ccgaagaagc tgaggtagct
      661 cggcagcgtg atgttgaaga agcggttgta gagggcgccg gcgatgaggc ccgagagaat
      721 gcccacggga acgctgagct tcgccagctc cttcgccttg taggcggcga cgccgagatc
      781 gcgcgcccgg cccgcgaggt cagcgaccgc gctgccgggc acgctgatca gcacctccgc
      841 acccttggtc gcaaccaggt aggcgaccac agcggcaagc ccggccgcgc cgtgattctc
      901 gcgcgccagc cccaccgcca cgccggcggc aaagagcagc cccagattgt cgaagatggc
      961 cccgccggcg gcggcgatgg aggcggagtt gagcaggtcc ggctgaccga gccgcagcag
     1021 caaccccgcg atcggcagca ccgcgatggg cagcatcagc gcgccaccga cccgctgcag
     1081 actcgcgagg aacgacctca ctgcgccgcc tccctgccct cgccgagcgc acgccgcgcg
     1141 agcatgcgca ccgccgcggc atcggccgct gcgagcgccg agcgggcgag tgcgcggcat
     1201 tccgcgacgg tgacgcggct caaattcgcc ttgagctgcg ggatgaccgc accgaccgcc
     1261 gaaagctcgt gcacgccaag accgacgagg agcggggccg cgaggggctc ggaggcgagc
     1321 ccgccgcaca cggcgaccat ccgtccgcgg gtagcggcgg cctgcgcggc ggtctcgatc
     1381 agccgcagca ccgccgggtg cagcgcgtcc agcctcggtg ccagctgcgg gtggccgcga
     1441 tccatggcga gcgtgtactg ggtaaggtca ttggtgccga tcgagatgaa atccgcgacc
     1501 tcgaggagcc ggtcggcgag cacggccgag gccggtgtct cgatcatgac gcccacctcg
     1561 atggcctgcg tcacgccgag ctcggcgcgc agctcctcta gtaccgcacg caccgcctgc
     1621 acttcggccg cctcggtga
//
LOCUS       DATLKL010000328         1858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_172889, whole genome shotgun sequence.
ACCESSION   DATLKL010000328 DATLKL010000000
VERSION     DATLKL010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172889"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VM713_04280"
     CDS             <1..770
                     /locus_tag="VM713_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HVH83518.1"
                     /translation="APATAASPPAAAPPPATAPAQSETEQARGAQESSDGAVNEKPES
                     SDASLERMASLPPDAQLPAGRWKPGVNYAPVVPGQPTSVEPGKVEVIEVMWLGCPHCY
                     ALEPYVKAWLKSKPPYIEFVQVPVIWQAQHQAHAKLFYTLKALGRLDLIDKAFQTIQQ
                     QRNPLIGNSDADSLRIEQAWATQNGVTASDFANAYNSFSVNSDLARAQELTQRYRIES
                     VPTVIINGKYITEESKAGSPAKLFQLIDDLAASERRH"
     gene            complement(775..851)
                     /locus_tag="VM713_04285"
     tRNA            complement(775..851)
                     /locus_tag="VM713_04285"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(815..817),aa:Arg,seq:ccg)
     gene            998..1519
                     /locus_tag="VM713_04290"
     CDS             998..1519
                     /locus_tag="VM713_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVH83519.1"
                     /translation="MSKQDTHFFNVFSLVIGLLVAVTIVLFALARIVASHTQEAQVLT
                     EPDYSKNVAARIAEPAHEAVAGADNSALAIKVETPAGAGSAALAIPKNGTEVFEQTCS
                     VCHGQGIAGAPKAGDKAAWAPRIAAGKATLYEHALKGFQGKTGVMPPKGGRTDLPDDL
                     VRQAVDHMVQLAQ"
     gene            complement(1527..>1858)
                     /locus_tag="VM713_04295"
     CDS             complement(1527..>1858)
                     /locus_tag="VM713_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83520.1"
                     /translation="GELDALPDAGGGSAAAAVAVSRARELQRARQGICNARLTDAEVR
                     RWCAPDEAGRQILEVAMQRRRLSARARARVLKVARTIADLDGRAALGAREVSEAVMLR
                     CLDRAAP"
BASE COUNT          322 a          673 c          614 g          249 t
ORIGIN      
        1 aagccccggc gacggcggcg agccctcccg ccgctgcgcc cccgccggcc accgcgcccg
       61 cccaaagcga gaccgagcag gcgcgcggcg cccaggaatc ctccgacggc gccgtcaacg
      121 agaaacccga gtcgagcgat gcctcgctcg agcgcatggc atcgctccct cccgatgcgc
      181 agctgccggc gggccgctgg aagcccggag tgaactacgc gccggtcgtg ccaggccagc
      241 cgaccagcgt cgagccgggc aaggtcgagg tgatcgaagt catgtggctc ggctgcccgc
      301 actgctacgc gctcgagccc tacgtcaaag cctggctcaa gagcaaaccg ccctacatcg
      361 agttcgtgca agtgccggtg atatggcagg cgcagcatca ggcccatgcc aagctcttct
      421 acacgctgaa ggcgctggga aggctggatc tcatcgacaa ggcgttccag accatccagc
      481 agcagcgcaa cccgctcatc gggaactccg atgccgattc gctccggatc gagcaggcgt
      541 gggcaacgca gaacggcgtc accgcctcgg acttcgccaa cgcctacaac tcgttttcgg
      601 tgaattccga tctggcgcgc gcccaggagc tgacgcagcg ctatcgcatc gagtcggtgc
      661 ccaccgtcat catcaacggc aagtacatca ccgaggagag caaggccggc agtcccgcca
      721 agctcttcca gctgatcgat gacctggcgg cgtccgagcg ccggcactag aggttggcgc
      781 gcccgactgg attcgaacca gcgacctgca gcttcggagg ctgccactct atccaactga
      841 gctacgggcg cgttcgggcg ggcgatgata cgcggggcca gaagggccgt ctaattgcgc
      901 gccgcatcgt ggcgcggcgc gccggcattt ccgcagccgc gaggcgagcg gctatactgc
      961 gccgcccgaa tcggcttggt gcgcggcgga aactcacgtg agcaagcagg acacgcattt
     1021 cttcaacgtt ttcagcctgg tgatcgggct gctggtggcc gtcaccatcg tgctgttcgc
     1081 gctcgcgcgc atcgtcgcca gccacacgca ggaagcgcag gtgctcaccg agccggacta
     1141 cagcaagaac gtggccgcgc gtatcgccga gccggcgcat gaggcggtcg cgggagcaga
     1201 caattccgcg ctcgccatca aggtggagac gcccgccggc gcaggctcgg cggcgctcgc
     1261 cattccgaag aacggcacgg aggtcttcga gcagacctgc agtgtgtgcc acggccaggg
     1321 aatcgccggc gcgcccaagg cgggcgacaa ggcggcgtgg gcgccgcgca tcgccgcggg
     1381 caaagcgacg ctctacgagc acgcgctcaa gggcttccag ggcaagacgg gtgtgatgcc
     1441 cccgaagggc ggccgcaccg acctgccgga cgacctcgtc aggcaggccg tcgatcacat
     1501 ggtccagctg gcccagtagc cgtgctctaa ggcgccgcgc ggtccagaca gcgcagcatc
     1561 acagcctcgc tcacctctct ggcgccgagc gccgcgcggc cgtcgagatc ggcgatcgta
     1621 cgtgcaacct tgagcacgcg cgcccgtgcc cgcgcggaca gccgccggcg ctgcatcgcc
     1681 acttccagga tctgccgccc cgcctcatcg ggcgcgcacc agcgacggac ctcggcatcg
     1741 gtgagccgcg cattgcagat cccctggcgc gcgcgctgca gctcgcgggc acggctcacc
     1801 gcgaccgccg ccgcagcgct gccgccgccg gcatccggca gcgcgtcgag ctcccccg
//
LOCUS       DATLKL010000329         2489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_217905, whole genome shotgun sequence.
ACCESSION   DATLKL010000329 DATLKL010000000
VERSION     DATLKL010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217905"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VM713_04300"
     CDS             complement(<1..736)
                     /locus_tag="VM713_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83521.1"
                     /translation="MSAPEPTSGNFDAIVVGSGISGGWAAKELTEKGLRTLVLEAGPP
                     IVPERDFVEHVQDYSLAYRGYGNQRKLHEHQPIQSQTGACDEWVSKWFVNDRENPYHN
                     DADKPFLWIRGRHVGGRSLMWGRQVYRWSDLDFEANAREGIGVDWPIRYADIAPWYSH
                     VERFIGVSGQAEGLAQLPDGEFLPPMQLNCAELQVRDSMRQRWGRERVLTIGRVAILT
                     QPHLGRAACHYCGPCWRGCITHSYFNS"
     gene            complement(723..1331)
                     /locus_tag="VM713_04305"
     CDS             complement(723..1331)
                     /locus_tag="VM713_04305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HVH83522.1"
                     /translation="MCGSVTCVDRREFLESVAGITALLPGLALLPLEQPAFAAGLHAR
                     AQAATALRTLGTAQNAAVSCIAEILLPQTDTVGATEVGVNRFIDLLLTDSMVERDRNR
                     FLEGLAAIDARSRALYGAPVASAHQDQQQSLLRSLDEQLPPRAPTKAEQALLDKQPVT
                     AERGYELLKRLVVFAYFTSAPVAKDLIKAPIIPGRYDGCVST"
     gene            1413..2339
                     /locus_tag="VM713_04310"
     CDS             1413..2339
                     /locus_tag="VM713_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HVH83523.1"
                     /translation="MKRISLALLAGVLLALSVAAQAVTVGVSMALFDDNFLTAVRASM
                     KERAQQLNVPVQFEDAENDIGRQLNHIQNFIAQHVDAIIVNPVDTDATPRMTQLAVAA
                     GIPLVYVNRMPGDRQLPPRVSFVGSDETQSGTLEMAEVCRLLRGKGDIVVLMGELTNQ
                     SARQRTQDIRDVLARPACHDIRVIDQQAANWKRTEAADLVTNWLSAGLHSAAVVANDD
                     EMAIGAIQALKQAHLLSTTIVAGIDATPDALAEMKAGGLAVTVFQNAPAQGRGAIDTA
                     LKLARHEPVAAFVWVPFELVTRANLAGYLGRH"
BASE COUNT          403 a          868 c          832 g          386 t
ORIGIN      
        1 tgctgttgaa gtaggagtgc gtgatgcagc cgcgccagca cggcccgcag taatgacacg
       61 cggcgcgccc gaggtgcggc tgggtgagga tcgccacgcg cccgatggtg agcacccgct
      121 cgcgccccca gcgctgccgc atcgagtccc gcacctgcag ctccgcgcag ttgagctgca
      181 tcggcggcag gaactccccg tccggaagct gcgccaagcc ctccgcctgg ccgctcacgc
      241 cgatgaatct ctcgacgtgg ctgtaccagg gcgcgatgtc cgcgtagcgg atcggccagt
      301 cgacgccgat gccctccctg gcgttggctt cgaagtcgag atcgctccag cgatagacct
      361 gccggcccca catgagggag cggccgccca cgtgacgccc gcggatccac aggaacggtt
      421 tgtcggcgtc gttgtggtag ggattctccc ggtcgttcac gaaccacttc gatacccact
      481 cgtcgcacgc gccggtctgc gactgaatcg gctggtgctc gtggagcttg cgctggttgc
      541 cgtagccccg gtacgcgagc gaatagtcct gcacgtgctc gacgaaatcc cgctccggca
      601 cgattggcgg tccggcctcg agcaccagcg ttctcaggcc tttctcggtg agctccttcg
      661 cggcccagcc gccggagatc cccgagccga cgacgatcgc gtcgaagttc cccgaggtcg
      721 gctcaggtgc tgacacagcc gtcgtacctt ccgggaatga tcggagcctt gatgaggtcc
      781 ttggcaacgg gcgcggaggt gaagtaggcg aagaccacca ggcgcttcag cagctcgtag
      841 ccgcgctcgg cggtcaccgg ctgcttatcg agcagcgcct gctctgcctt ggtcggagcc
      901 cgcggcggca actgctcgtc gagcgagcgg agcagcgact gctgctggtc ctggtgcgcg
      961 gaggcgaccg gtgcgccgta gagcgcgcgg ctgcgcgcgt cgatggccgc gagcccctcg
     1021 aggaagcggt tgcgatcgcg ctccaccatc gagtcggtga ggagcagatc gatgaagcgg
     1081 ttcacgccca cctcggtcgc cccgacggtg tcggtctgcg gcaggaggat ctcggcgatg
     1141 cagctgaccg ccgcgttctg cgccgtgccg agcgtgcgca gcgccgtcgc ggcctgcgcg
     1201 cgcgcgtgca gcccggccgc aaacgccggc tgctcgagcg gcaggagcgc cagccccggg
     1261 agcagcgcgg taatgcccgc gacggattcg aggaactcgc gccgatccac gcaggtgacg
     1321 ctaccacaca tggagacccg acggttagag tcgcggccgg cccccgcatt agaatgagcg
     1381 ctacgctcgg ctatcctgca aaggacttga gcatgaagcg catttcgctg gcgctgctcg
     1441 ctggtgtgct gctggcactg tccgtcgctg cgcaggcagt cacggtgggc gtgtccatgg
     1501 ctctgttcga cgacaacttc ctgacagcgg tgcgcgccag catgaaggag cgcgcccagc
     1561 agctcaacgt cccggtgcag ttcgaggacg ccgagaacga catcggccgg cagctcaacc
     1621 atatccagaa cttcatcgcg caacacgtcg acgccatcat cgtcaatccc gtcgacaccg
     1681 acgcgacgcc gcgcatgacg caactggccg tcgccgccgg gatcccgctc gtatacgtca
     1741 accgcatgcc gggcgaccgg cagctgccgc cgcgcgtctc cttcgtcggc tcggacgaaa
     1801 cccagtccgg gacactggag atggccgaag tctgccgcct gctgcgcggc aagggcgaca
     1861 tcgtcgtact gatgggcgag ctcaccaacc agtcggcgcg ccagcgcacc caggacatcc
     1921 gcgacgtgct cgcgcggccc gcgtgtcacg acatccgcgt gatcgaccag caggccgcca
     1981 actggaagcg caccgaggcg gccgacctcg tgaccaactg gctgagcgcg gggctgcatt
     2041 cggccgccgt cgtcgccaac gacgatgaga tggcgatcgg cgctatccag gcgctcaagc
     2101 aggcgcacct cctcagcacc accatcgtcg ccggaataga tgccaccccc gatgcgcttg
     2161 cggagatgaa ggccggaggg ctcgcggtga cggtcttcca gaacgcgccg gcgcagggcc
     2221 gtggcgccat cgacaccgca ctcaagctcg cgcggcacga gcccgtggcc gccttcgtgt
     2281 gggtgccgtt cgagctggtg acgcgcgcca acctcgccgg ttacctcgga cgtcattgat
     2341 ggactcagga ttgatgggcc caggcgccgc cgctgccatg ccgctcgacc tgctcgaggt
     2401 gagcagggtc tgcaaggcct tccccggtgt cacggcgctc gaggacgtga cgctccacct
     2461 caagcacggc tcggtgcacg cactgatcg
//
LOCUS       DATLKL010000330         2342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_48757, whole genome shotgun sequence.
ACCESSION   DATLKL010000330 DATLKL010000000
VERSION     DATLKL010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48757"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1813)
                     /gene="lepA"
                     /locus_tag="VM713_04315"
     CDS             complement(17..1813)
                     /gene="lepA"
                     /locus_tag="VM713_04315"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HVH83524.1"
                     /translation="MRHIRNFSIIAHVDHGKSTLADRFIQLCGGLADREMQEQVLDSM
                     DLERERGITIKAQSVTLYYSARDGERYQLNMIDTPGHVDFSYEVSRSLAACDGALLVV
                     DAPQGVEAQSVANCNTAIEQGLEVLPVINKIDLPSADPERVMREIEEIIGIEAVDAAR
                     VSAKTGEGVAELLEVLIRRIPAPRGDGAAPLQALIIDSWFDNYVGVVSLVRVVNGSLK
                     SGDRIRVVSTGRSHYVDRLGRFTPKAVVEEELATGDVGFVVAGIKEIDGAPVGDTITL
                     ENRPASTPLPGFKQIKPRVFAGLFPVNSEDYEKFRDALSKLRLNDSALHYEPEVSGAL
                     GFGFRCGFLGLLHMDIVQERLEREYQLTLVTSAPTVVYEVAATDGTVEYVDNPAKLPP
                     ADEIEELREPVIIANILVPPDHVGAVLKLCNDKRGTQNRMLYLGTQVSLQYQLPLAEV
                     VLDFFDRLKSASRGYASFDYEFSHFQAAPLVKLDIPINGDKVDALSIIVHKESAYQRG
                     RELVDKMQELIPRQMFEVAVQAAIGSAVIARATVKALRKNVLAKCYGGDVTRKRKLLE
                     KQKEGKKRMKQLGHVEIPQEAFLAVLQVGRKH"
     gene            complement(1923..2201)
                     /locus_tag="VM713_04320"
     CDS             complement(1923..2201)
                     /locus_tag="VM713_04320"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HVH83525.1"
                     /translation="MSPQPEPAARLTLVQRHGCELCETMAQDLAALARRLTLPPIELI
                     DVDSDPQLRRRHGLDVPVLLLDGLVVCRHRLDAAELARLLRLAPPGGR"
BASE COUNT          355 a          757 c          821 g          409 t
ORIGIN      
        1 catttggtgc gcgcccctag tgcttgcggc cgacttgcag caccgcgagg aatgcctcct
       61 gcgggatctc gacgtggccg agctgtttca tgcgtttctt gccttctttc tgcttctcga
      121 ggagcttgcg cttgcgggtg acgtcgccgc cgtagcactt tgccagcacg ttcttgcgca
      181 gcgccttgac ggtggcgcgg gcgatgaccg cgctgccgat ggcggcctgc accgcgactt
      241 cgaacatctg ccgcggaatg agctcctgca tcttgtcgac cagctcgcgg ccgcgctggt
      301 aggcgctctc cttgtgcacg atgatcgaca gggcgtccac cttgtcgccg ttgatgggaa
      361 tgtcgagctt gacgagcggc gcggcctgga agtggctgaa ttcgtagtcg aaggaggcgt
      421 agccgcggct cgcggacttc aggcggtcga agaaatcgag caccacctcg gcgagcggca
      481 gctggtattg cagcgacacc tgggtgccga ggtacaacat ccggttctgt gtgccgcgct
      541 tgtcgttgca gagcttgagc acggcgccga catgatcggg gggcaccagg atgttggcga
      601 tgatgaccgg ctcgcgcagc tcctcgatct catccgccgg cgggagcttg gcggggttgt
      661 cgacgtactc gaccgttccg tcggtggccg ccacctcgta gaccacggtc ggcgcgctgg
      721 tgacgagggt gagttgatac tcgcgctcga ggcgctcctg gacgatgtcc atgtgcagca
      781 gaccgaggaa accgcagcgg aagccgaagc cgagcgcgcc ggagacctcc ggctcgtaat
      841 ggagcgcgga gtcgttgagg cgcagcttcg agagcgcgtc gcggaacttc tcgtagtcct
      901 ccgagttcac cggaaagagc ccggcgaaca cccgcggctt gatctgcttg aagccgggga
      961 gcggggtact cgcagggcgg ttctccagcg taatggtgtc gccgaccggg gcgccgtcga
     1021 tctccttgat gccggcgacc acgaagccga cgtcgcccgt ggcgagctcc tcctcgacca
     1081 ccgccttggg ggtgaagcgg ccgaggcggt ccacgtaatg gctgcggccg gtcgagacga
     1141 cacggatccg gtcgccgctc ttcaggctcc cgttcaccac ccgcaccagc gatacgacgc
     1201 ccacgtagtt gtcaaaccac gagtcgatga tcagcgcctg caacggcgcg gcgccgtcgc
     1261 cgcgcggcgc ggggatgcgc cggatcaaca cctcgagcag ctcggcgact ccctcgccag
     1321 tcttggcgct cacgcgcgcg gcatccaccg cctcgatgcc gatgatctcc tcgatctcgc
     1381 gcatcacccg ctcggggtcc gccgacggca ggtcgatctt gttgatgacc ggcagcacct
     1441 cgagcccctg ctcgatcgcc gtgttgcagt tggcgacgct ctgggcctcc acgccctggg
     1501 gggcgtcgac caccagcaat gccccatcgc atgctgcgag cgagcgcgac acctcgtagg
     1561 agaagtcgac gtgccccggg gtgtcgatca tgttgagctg atagcgctca ccgtcgcggg
     1621 cgctgtagta gagcgtgacg ctctgtgcct tgatggtgat gccgcgctcg cgctccaggt
     1681 ccatggagtc gagcacctgc tcctgcatct cgcgatccgc gaggcccccg cacagctgga
     1741 tgaaccggtc ggcgagggtc gatttgccgt ggtctacgtg cgcgatgatg gagaagttac
     1801 gtatgtgccg catgaatcgg ggaagccgcc gggagggaac ccacgccgtg gtccaagtcc
     1861 agcggcgggc ggattatagg gaagtttgcg ggttctggcc cgccgcactg ggtgcgctta
     1921 tgtcagcggc cgcccggggg cgccagccgc agcagccgtg cgagctcggc ggcgtcgagc
     1981 cggtggcggc acaccaccag gccgtcgagc agcagcaccg ggacgtcgag accgtggcgg
     2041 cgccgcagct gcggatcgga atcgacgtcg atgagctcga tgggcggcag ggtgagcctg
     2101 cgggcgagtg cggcgaggtc ctgcgccatg gtctcgcaca gctcgcagcc gtgccgctgc
     2161 accagcgtga ggcgcgcggc cggctccggc tgcggtgaca tgggcttcag cggcgggtgg
     2221 ccgcgggcag cgtcgcctct tcggccttga cctggctggc gatgaaacgc acggtcgccg
     2281 gcggcacctc gcccatggca gtgatcttgt agccgttcat caccgtcgag aacgccgagg
     2341 ag
//
LOCUS       DATLKL010000331         1592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_217979, whole genome shotgun sequence.
ACCESSION   DATLKL010000331 DATLKL010000000
VERSION     DATLKL010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217979"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VM713_04325"
     CDS             <1..712
                     /locus_tag="VM713_04325"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVH83526.1"
                     /translation="FQREIQQGDRFEVAFERLADGRGQVAKTGDIVYAALTLSGHTLK
                     IYRYTPRGGMADYFNERGESVRKALLRTPVDGARLTSGFGMRMHPLLGYSTMHRGVDF
                     GAAPGSPIMAAGDGIIDMAGWNGGYGNYVRIRHNERYSTAHAHMSRIANGIYKGSHVR
                     QGQVIGYIGMTGLATGPHLHYEVLLHNQQINPLSVKLPTGIKLVGPELKAFTEARAKT
                     DAAVAALPPVDKLAQTAF"
     gene            complement(818..>1592)
                     /locus_tag="VM713_04330"
     CDS             complement(818..>1592)
                     /locus_tag="VM713_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVH83527.1"
                     /translation="VGKTELTKALAEFLFDDESALIRIDMSEYMEKHSVARLIGAPPG
                     YVGYDEGGSLTEAVRRRPYQVILFDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFR
                     NTLIVMTSNLGSDILAAQPPGQDVDAVRAQVMEIVRQHFRPEFLNRLDEILLFHRLMR
                     EQMAGIVEIQLGRLRKLLAERKITLTLDERARAWLAEAGYDPVYGARPLKRVIQRNLQ
                     NPLASLILEGKVRDGEEVRVTVKGGRLAINGVTVIEKAA"
BASE COUNT          289 a          553 c          483 g          267 t
ORIGIN      
        1 tttccagcgc gagatccagc agggcgatcg cttcgaggtc gccttcgagc ggctcgccga
       61 cggacgcggc caagtcgcga agaccggcga cattgtctac gccgccctca cgctctcggg
      121 tcacacactc aaaatctacc gctacacccc caggggtggg atggctgact acttcaatga
      181 gcggggcgag agcgtgcgga aagcgctctt gcgtaccccc gtcgacggcg cccggctcac
      241 ctcgggcttc ggcatgcgca tgcacccgct gctgggctac agcacgatgc atcgcggcgt
      301 ggacttcggc gcagcccctg gctcgccgat catggcggca ggtgacggaa tcatcgacat
      361 ggccggctgg aacggcggct acggcaacta tgtccgcatc cgtcacaacg agcgatacag
      421 cacggcccac gcacatatga gccgaatcgc taacggaatt tacaaaggtt cccacgtgcg
      481 gcagggccag gtcatcggct atatcggcat gaccggcctc gccaccggcc cgcacctcca
      541 ttacgaggtg ctgttgcaca accagcagat caacccgcta agcgtgaagc tgccgaccgg
      601 cataaagctc gtcggccccg agctcaaggc cttcaccgag gcgcgcgcca agacggacgc
      661 cgctgtcgca gcgctgccac ccgtggacaa actcgctcag accgcattct gacctgtcgc
      721 ccgaggcacc gctcggaaga tcacgcgaag gttcgctctc ggccggtcca cgggcgcagt
      781 tcaacctcga atgccggggc cgccgcgacc cgctcgctca ggccgccttc tcgatcaccg
      841 tgacgccgtt gatggctagc cggccgccct tgacggtcac gcgcacctcc tcgccgtcgc
      901 gaaccttgcc ttcgaggatc aagctcgcga gcgggttctg caggttccgc tgaatcacac
      961 gtttcagcgg cctggcccca tagaccggat cgtacccagc ctcggcgagc caggcgcgcg
     1021 cccgctcgtc gagggtgagt gtgatcttgc gctcggcgag cagcttgcgc aggcggccca
     1081 gctggatctc gacgatcccg gccatctgct cgcgcataag tcggtggaac agcagaatct
     1141 cgtcgagccg attcagaaat tctggccgga aatgctggcg cacgatctcc atcacttgcg
     1201 cgcgcaccgc gtccacgtcc tggcccggcg gctgggcggc aagaatgtcg ctgcccagat
     1261 tcgaggtcat gacgatcaag gtgttgcgaa aatcgaccgt gcggccctgg ccgtccgtca
     1321 ggcgaccgtc gtcgagcacc tgcagcagca cgttgaagac atccgggtgg gccttttcga
     1381 cctcgtcgaa caggatcacc tgatacggcc ggcgccgcac cgcctcggtc agcgagccac
     1441 cctcgtcgta gccgacatag cccggaggtg ccccgatcaa acgggcaacc gagtgcttct
     1501 ccatgtattc cgacatgtcg atgcggatca gcgcgctctc gtcgtcgaac aggaattcgg
     1561 cgagcgcctt tgtcagctcg gtcttgccga cg
//
LOCUS       DATLKL010000332         2274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_48830, whole genome shotgun sequence.
ACCESSION   DATLKL010000332 DATLKL010000000
VERSION     DATLKL010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48830"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VM713_04335"
     CDS             <1..493
                     /locus_tag="VM713_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVH83528.1"
                     /translation="SADLGPLISRAHRDKVLFYYRLAREEGGEIVCGGGVPQLGDERD
                     AGAFIEPTILRGLAEQARCVREEIFGPVCHVAPFDSEEEVVRLANDTRYGLCAAVWTS
                     NLQRAHRVARQMEVGITWVNDWYLRDLRTPFGGAKLSGIGREGGMHSLAFFSEPMNIC
                     VKL"
     gene            490..966
                     /locus_tag="VM713_04340"
     CDS             490..966
                     /locus_tag="VM713_04340"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVH83529.1"
                     /translation="MMTSTKSAISGGVAAASATGDSGVLAGKARPRGTFPHFRRAGDF
                     IFISGTSARRPDDSIAGAEPGHDGTPRLDIRAQTRSVIENIRDILAAAGASLADVVEV
                     STFLVSMDDFKGYNEVYSEFFGFEGPARTTVAVAQLPHPHLLIEIKAVAWHPRNGA"
     gene            993..2030
                     /locus_tag="VM713_04345"
     CDS             993..2030
                     /locus_tag="VM713_04345"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVH83530.1"
                     /translation="MSIDIHTHFLPRTWPDLAARFGTPDWPWMRHGPGGKAMLMVGER
                     EFRPVSQACWDARLRLEHMDRDGVEVQIMCATPLLFAYERPAAQTLECARIFNDAALE
                     ICRHAPRRLKSLCQVPLQDTELACRELSRAMDDGHIGVHIGNHVGAREMNDEGILTFL
                     RHCAGEGAAVFVHPWDMMAQERMPKYQLPWLVSMPAETQLSILWLILSGAFERLPRSL
                     KLCFAHGGGSFPYLLGRVDNAWRNRDIVREDCPHPPSSYLDRFSVDGAVFSHDALEFL
                     VRTMGEDRVMLGSDYPYPLGEQGVGTLVRSHPQLTAATRAKLLQENAAAFFGIEAGRP
                     TRAGSGAQLSV"
BASE COUNT          355 a          779 c          772 g          368 t
ORIGIN      
        1 gagcgcggac ttgggtcctc tcatctcccg ggcccatcgc gacaaggtgc tcttctacta
       61 tcgcctggcg cgcgaggagg gcggcgagat cgtctgcggg ggcggcgtgc cacagcttgg
      121 cgatgagcgc gacgcgggcg cgttcatcga gccgaccatt ctgcgcggcc tcgccgaaca
      181 ggcgcgctgc gtgcgcgagg agatcttcgg ccccgtctgc cacgtggcgc cgttcgattc
      241 cgaggaggaa gtggtgcgcc tcgcgaacga cacgcgctac gggttgtgtg ccgcggtctg
      301 gacttcgaat ctgcaacgcg cccaccgggt cgcgcggcag atggaagtcg gcatcacctg
      361 ggtgaacgac tggtacctgc gcgacctccg caccccattc ggcggcgcga agctctccgg
      421 catcgggcgc gaaggcggca tgcattcgct cgcctttttc tccgagccga tgaatatctg
      481 cgtgaagcta tgatgacctc cacgaagagc gcgatcagcg gcggcgtcgc ggcggcgtcc
      541 gcgaccggcg acagcggcgt gctcgccggc aaggcgcggc cgcgcggcac cttcccgcac
      601 tttcgccgcg ccggggactt catcttcatc tccggcacga gcgcgcgccg ccccgacgac
      661 tcgatcgcgg gcgccgagcc cggtcacgac ggcacgccgc ggctcgacat ccgcgcgcag
      721 acgcgcagcg tcatcgagaa catccgcgac attctcgccg ccgccggcgc cagcctcgcc
      781 gacgtcgtcg aggtgagcac gttcctcgtc agcatggacg acttcaaggg ttacaacgag
      841 gtgtacagcg agttctttgg cttcgaaggt ccggcgcgca ccaccgtggc ggtggcacag
      901 ctgccgcacc cgcacctgct gatcgagatc aaggcggtgg cctggcaccc gcgcaacggc
      961 gcgtgagcgc catttcgagg agagacacag ccgtgagcat cgacatccac acacacttcc
     1021 tgccgcgcac ctggccggat ctcgcggcgc gtttcggcac acccgactgg ccgtggatgc
     1081 ggcacggccc cggcggcaag gcgatgctca tggtcgggga gagggagttc cgcccggtca
     1141 gccaggcctg ctgggacgcg cggctgcgcc tcgagcatat ggacagggac ggggtggagg
     1201 tgcagatcat gtgtgccaca ccgctgctgt tcgcctacga gcgcccggcc gcgcagacgc
     1261 tcgaatgcgc gcgcatcttc aacgatgcgg cgctcgagat ctgcaggcac gcgccgcggc
     1321 ggctgaagtc cctgtgccag gtgccgctgc aggacacgga gctcgcctgc cgcgaattga
     1381 gccgcgcgat ggacgacggt cacatcggcg tgcacatcgg caaccatgtc ggggcacggg
     1441 agatgaacga cgaagggatc ctcaccttcc tcaggcactg cgccggggag ggcgcggcgg
     1501 tgttcgtgca tccgtgggac atgatggcgc aggagcggat gcccaagtac cagctgccgt
     1561 ggctggtgtc catgccggcc gagacgcagc tgtcgatcct gtggctgatt ctctcggggg
     1621 ccttcgagcg gctgccgcgc tcgctcaagc tctgcttcgc gcacggtggc ggcagcttcc
     1681 cgtacctcct cggccgcgtc gacaatgcct ggcgcaaccg cgacatcgtg cgcgaggact
     1741 gcccgcaccc cccttcatcg tacctcgacc gcttctcggt cgacggcgcg gtgttcagcc
     1801 atgacgcgct cgagttcctg gtacgcacca tgggcgagga ccgcgtcatg ctcgggtccg
     1861 actatcccta tccgctcggt gagcagggag tcggaactct ggtgcgctcg cacccccagc
     1921 tcactgcggc gacgcgcgcg aagctcctgc aggagaatgc ggccgcgttt ttcgggattg
     1981 aggccggccg gccgacccgc gccggctccg gcgcgcagct gagcgtctag tcatattggc
     2041 ccaaatcgca ggaagcgatt tgggcggacc tcactccgtc ggtcgccggc aaaaagcggg
     2101 gtttttgccg gctgcgcgcg gccaaaccgt tatgatcgac ggccatgact tcggcccggc
     2161 tcgagcggtg gatcacgcgc aagttccgtc tgcgacacgt cgagctgatc gcggagctgc
     2221 atgactgccg cagcatcctc aaggcctcga aacgcctgag tctcgcccag ccta
//
LOCUS       DATLKL010000333         4087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_71662, whole genome shotgun sequence.
ACCESSION   DATLKL010000333 DATLKL010000000
VERSION     DATLKL010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71662"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VM713_04350"
     CDS             complement(<1..943)
                     /locus_tag="VM713_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVH83531.1"
                     /translation="MQTQLLIGGRLVGGEGPPETVLDAASGAAIATVASAGVEQVDAA
                     VAAAAAAFDGWAGTPPRERATQLLRIADGIEREAAAYASLESRNTGKPLQAMLNDEMP
                     AIADVFRYFAGACRIPQGLAAGEYLAGHTSLIRRDPVGVVASIAPWNYPLMMAAWKIA
                     PAIAAGNTLVLKPSEQTPLTALKLGEQLAGHLPAGVVNIVCGRGEPVGTALTSHPRVR
                     MVSLTGDVGTGQKVLAAAAGNLKHTHLELGGKAPVIVFDDADLEAVVSGVRTFAFYNA
                     GQDCTAACRLYAGERIYERLVADLTGAARSIRVGAQQE"
     gene            1050..1949
                     /locus_tag="VM713_04355"
     CDS             1050..1949
                     /locus_tag="VM713_04355"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HVH83532.1"
                     /translation="MNSDVIITCAVTGDDTKVTKSPHCPITPEQIAAAAVEAARAGAA
                     IVHIHVRDPETGAFSMATRHYREVVKRVRDAKVDVLINLTCGMGGYICVGEQGLADTP
                     AEGTDFVSQEERMRHVIDLCQEGLYRPDIATLDCGSLNFGDGNRAYVSTPSYLRKGAA
                     ILRDLRVKPELEVFDTGNLWFVKQLLKEELIGAPALIQLCTGIPYGVPADTGHLLAMV
                     NYLPAGSVWTSFAVSRMQMPWVAQSVLLGGNVRVGLEDNLYLSRGVYASNAQLVEKAR
                     TIVEAMGARVVSAAEARDRLKLH"
     gene            1969..2454
                     /locus_tag="VM713_04360"
     CDS             1969..2454
                     /locus_tag="VM713_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVH83533.1"
                     /translation="MVGIPVYETALQPEWIDYNGHVRDAYYGLIASSACDALMDRLGL
                     DAAYRARTRCTLYTLEMHVHYLHEVTQGERIAVDVIILGSDHKRLHAGFTLRRAPDGP
                     VAATVEFMLLHVHQGDTPGSEPFPPSVTSAIAELAAAAPAAAFGPGSRALSLRGGARS
                     G"
     gene            2451..>4087
                     /locus_tag="VM713_04365"
     CDS             2451..>4087
                     /locus_tag="VM713_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HVH83534.1"
                     /translation="MNPRALQRLLAPRSVALIGGEWADAVAAASRRIGYRGELWRVHP
                     ARPSSAAQHYFRSVEELPGAPDAAFIAAPSREVPAIAAALAARGAGGFVCFAAGFSES
                     GTEAGRRLTAELLAGAADLPFFGPNCYGFINFFDGVALWPDQVVGGRRDRGVALICQS
                     GTIALNLLFNSRSLPIGCLLTVGNQTRLAAEDLLELLAADERVTAFGLYLEGVKDIAR
                     FARAVMTARAAGKPIALVKAGRTAAAARTAHTHTGALAGADAAFDAFCRQAGIARCDS
                     LGTLCETLKVFHCGGPLAGRRVLVMGASGGDMAMTADNSRHLGLEFPPFPEASAARLR
                     EILSDRVSIANPFDFQTYVWFDRPRMREMFKVVQRAGFDAVGFTIDCPPPGEADASAY
                     LNGIEEFIAAIPGASSRGAVISSLPESLAAATRESCLRAGVVPLQGQREALEALDLAG
                     AVGEAWARGATPELRRPSGLKERARAIGEPEAKAALAAYGVHVPRSAVVRPRDAAGAA
                     EALGFPVVIKATGAALEHKSEVGGVVLNVRSALEATQA"
BASE COUNT          588 a         1465 c         1392 g          642 t
ORIGIN      
        1 cttcctgctg tgccccgacg cgaatgctgc gcgccgcgcc ggtgagatcc gcgaccagcc
       61 gctcgtagat gcgctcgccc gcatacaggc ggcaggccgc cgtgcagtcc tgcccggcat
      121 tgtagaaggc gaaggtgcgc acgcccgaca ccaccgcctc gaggtcggca tcatcgaaga
      181 cgatcaccgg cgccttgccg ccgagctcga gatgggtgtg cttgagattg ccggcggcgg
      241 cggccagcac cttctgcccc gtgccgacgt ctccggtgag cgagaccatg cgcacgcgcg
      301 gatggctcgt gagcgcggtg ccgaccggct cgccgcgccc gcagacgatg ttgacgacac
      361 cggcgggcaa gtgcccggcg agctgctcgc cgagcttgag cgccgtgagc ggggtctgct
      421 cggagggttt gaggaccagc gtgttgccgg cggcgatagc cggggcgatc ttccaggccg
      481 ccatcatcag cggatagttc cacggcgcga tcgaggcgac gacgccgacc gggtcgcggc
      541 ggatcaggct ggtgtgtccc gcgagatact cccccgccgc cagtccctgc ggtatgcggc
      601 aggctcccgc gaagtagcgg aatacatctg cgatcgccgg catctcgtca ttgagcattg
      661 cctgcagtgg cttgccggtg tttctggact cgagactcgc gtaggccgcg gcctcgcgct
      721 cgatcccgtc ggcgatcctc agcagctgcg tcgcacgctc cctgggtggc gtgccggccc
      781 agccgtcgaa ggccgccgcg gcagccgcca ccgccgcatc gacctgctcg acgcccgccg
      841 acgccacggt ggcgatggct gcgccgctcg ctgcatcgag taccgtctcc ggcgggcctt
      901 cgccccctac cagccggccg ccgattaaca attgagtctg catgggtcgc tccttcgcgc
      961 cggcgcgtcc gcttgccgca ccgcgccgcc gcgctatatt attatcgcca taatattatc
     1021 acgtcacccg cgccgcgctc gaggccgaca tgaactccga cgtcatcatc acctgcgccg
     1081 tcaccggcga cgacaccaag gttaccaaga gtccgcactg cccgatcacg cccgagcaga
     1141 tcgccgccgc ggcggtcgag gcggcgcgcg cgggagccgc catcgtgcac attcacgtgc
     1201 gtgatcccga gaccggggcc ttcagtatgg cgacgcgcca ttaccgcgag gtcgtcaagc
     1261 gcgtacgcga cgccaaggtg gacgtcctca tcaatctcac ttgcggcatg ggcggctata
     1321 tctgcgtggg cgaacagggg cttgccgaca cgcccgccga gggcacggac ttcgtcagcc
     1381 aggaagagcg catgcgacac gtgatcgacc tgtgccagga ggggctctac cgtcccgaca
     1441 tcgcgacgct cgactgcggc tcgctcaact tcggtgacgg caatcgcgcc tacgtctcga
     1501 cacccagcta cctgcgcaag ggcgccgcca tcctgcgcga cctgcgcgtg aagccagagc
     1561 tcgaggtgtt tgacaccggc aacctgtggt tcgtgaagca gttgctcaag gaagagctca
     1621 tcggcgcgcc ggcgctcatt cagctgtgca ccgggatccc ctacggcgtg cctgccgata
     1681 ccggccacct gctcgccatg gtcaattacc tgccggcggg gagcgtctgg acctcgttcg
     1741 ccgtgagccg catgcagatg ccgtgggtgg cccagtcggt gctgctcggc ggcaacgtgc
     1801 gcgtcggact cgaggacaat ctgtatctga gccgcggcgt gtacgcgagc aacgcgcagc
     1861 tggtcgagaa ggcgcgcacc atcgtcgagg ccatgggcgc gcgggtggtc agcgccgccg
     1921 aagcacgcga ccgtctcaag ctccactgaa caggtgtgaa ttcccgtcgt ggtcggaatt
     1981 cccgtatacg aaacggcgct gcagccggag tggatcgact ataacggtca cgtccgcgat
     2041 gcgtattacg ggctgatcgc cagcagcgcc tgcgacgccc tgatggaccg gctgggactc
     2101 gacgcggctt atcgcgcgcg cacccgctgc accctctata ccctggagat gcacgtgcat
     2161 tacctgcacg aggtgacgca gggcgagcgg atcgccgtgg acgtcatcat cctcggcagc
     2221 gatcacaagc ggctgcacgc cggcttcacg ctgcgccgtg ccccggacgg gccggtggcc
     2281 gccaccgtcg agttcatgct gctgcacgtg caccagggcg acactccagg gtccgagccg
     2341 tttccgccca gcgtaacttc ggccattgcg gagctcgctg cggccgctcc ggccgccgcc
     2401 ttcggcccgg gatcacgggc gctcagcctg cgcggcggcg cgcgcagcgg atgaatccgc
     2461 gcgccctgca gcgcctcctg gcaccgcgca gcgtcgcgct gatcggcggc gagtgggctg
     2521 acgcggtggc cgcggccagc cgcaggatcg gttatcgcgg cgagctgtgg cgcgtgcatc
     2581 ccgcgcgccc gtcgagcgcg gcgcaacact acttccgctc ggtcgaggag ctccccggcg
     2641 ccccggacgc ggccttcatc gccgcgccga gtcgcgaggt tccggcaatc gctgcggccc
     2701 tggcagcgcg cggcgcgggc ggtttcgtgt gcttcgctgc gggcttttcc gagagcggca
     2761 cggaggcggg acggcgtctc accgccgagt tgctcgcggg cgcagccgac ctgcccttct
     2821 tcggcccgaa ctgttacggc tttatcaact tcttcgatgg ggtagcactc tggcccgacc
     2881 aggtggtggg cgggcgccgc gaccgcggcg tggcgctcat ctgccagagc ggcaccattg
     2941 cgctgaatct cctcttcaac agccgctcgc tcccgatcgg ctgcctgctc acggtcggca
     3001 atcagacccg cctcgccgcc gaagacctgc tggagctcct ggccgccgat gagcgcgtga
     3061 ccgcgttcgg cctctatctc gagggcgtca aggacatcgc gcgcttcgcc cgtgccgtca
     3121 tgaccgcgcg cgccgccggc aagcccatcg cgctcgtcaa agccgggcgt acggcggccg
     3181 cggcgcgcac cgcccacact cataccggcg cgctcgccgg cgccgacgcc gccttcgatg
     3241 ccttctgccg gcaggcgggg atcgcccgct gcgattcgct cggcacgctg tgcgagacgc
     3301 tgaaggtatt ccattgcggc ggaccgctcg cgggccggcg cgtgctcgtc atgggcgcat
     3361 cgggcggcga catggcgatg accgccgaca attcccgcca cctcggactc gagttcccgc
     3421 cgtttcccga ggcgagcgcc gcacggctgc gcgaaattct ctccgaccgc gtgtcgatcg
     3481 cgaacccctt cgatttccag acctatgtgt ggttcgatcg cccgcgtatg cgcgagatgt
     3541 tcaaggtggt acagcgcgcg ggcttcgatg cggtcggctt tacgatcgac tgcccgccgc
     3601 ccggtgaggc cgacgcgagc gcctacctga acggcatcga agagttcatc gcggcgatcc
     3661 cgggcgcatc gagccgcgga gcggtgatct cctcgctccc cgagtcgctc gccgccgcga
     3721 cccgcgagag ctgtctgcgc gccggcgtcg tgccgctgca ggggcagcgc gaggcgctcg
     3781 aggccctcga tctggcgggc gcggtaggcg aggcgtgggc gcgcggggcg acgcccgagc
     3841 tgcgccggcc gagcggcctg aaggagcgcg cccgcgcgat cggcgagccc gaagcgaagg
     3901 cggcactcgc cgcctacggc gtgcacgtgc cccgttcagc ggtcgtgagg ccgcgcgatg
     3961 ccgccggcgc cgccgaagcc ctcggctttc ccgtggtgat caaggccacg ggcgcagcgc
     4021 tcgaacacaa gtccgaggtc gggggcgtgg ttctcaacgt gcgcagcgcc ctcgaggcga
     4081 cccaggc
//
LOCUS       DATLKL010000334         1888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_3704, whole genome shotgun sequence.
ACCESSION   DATLKL010000334 DATLKL010000000
VERSION     DATLKL010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3704"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VM713_04370"
     CDS             complement(<1..672)
                     /locus_tag="VM713_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVH83535.1"
                     /translation="MAANKQRAVVSGGAGFIGSHLCEALLDRGMRVLALDNFITGTPE
                     NLRHLVGNPDFELKQADVNHGVFIGGDVRYVLHFASPASPPQYDANPIHTLKVGTVGT
                     MNMLGLARAKGATFLLASTSEVYGDPLVHPQPETYWGNVNPIGPRGCYDEAKRCAEAF
                     AMAYHRAHDVDTRIVRIFNTHGPRMQVHDGRAVPNFMAQAIRGEPVTVYGDGMQTRSL
                     CYVSDL"
     gene            complement(659..>1888)
                     /locus_tag="VM713_04375"
     CDS             complement(659..>1888)
                     /locus_tag="VM713_04375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HVH83536.1"
                     /translation="LGNDVTLVDIDQGRIALLKRYQVPFFEPGMRELVERNARAGRLT
                     FTTSYKDAVPGAEFAIIAVSTPEGEGGEADLSHVESAASSIADAMEGPLIVVNKSTVP
                     PLTGDMVSGVLTKGNTKHRTAVVSNPEFLREGSAIQDFMHPDRVVIGSNDRQAAEKVA
                     GLYAPLDAPILVMPDIYTAEMIKYASNAFLATRISFINEIAWISERVGADAKLVAEGM
                     GLDKRIGQHYLDAGIGWGGSCFPKDVAALAAISERFNYHPQLLHAVMEINRDQRMVVI
                     DLLRELLDELPGRVVGLLGLAFKPNTDDMREAPSVDIAKVLIAAGAEVRAYDPAAIET
                     AKRVLPEIEYGRDAYEVASGADAVVLVTEWNEFRRLDMARLKQVMRRPVIVDGRNIYD
                     PAVMRSLGFTYRGIGRE"
BASE COUNT          309 a          610 c          616 g          353 t
ORIGIN      
        1 caggtcggag acgtagcaga ggctccgggt ctgcatgccg tctccgtaga ccgtgaccgg
       61 ttcgcctcgg attgcctggg ccatgaagtt cgggaccgcg cggccgtcgt gcacctgcat
      121 gcgcggaccg tgggtgttga agatgcgcac gatgcgggtg tcgacgtcgt gcgcgcggtg
      181 gtatgccatc gcgaaagcct cggcacagcg cttggcctcg tcgtagcagc cccttggccc
      241 gatcgggttg acgttgcccc aatacgtctc cggttgcgga tgcacgagcg ggtcgccgta
      301 aacctcggag gtcgaggcga gcagaaaggt ggcgcctttc gcgcgcgcca ggccgagcat
      361 gttcatcgtg ccgaccgtgc cgaccttcaa tgtgtgaatc gggttggcgt cgtattgcgg
      421 cggggaggcg ggtgaggcga agtgcaggac gtagcgcacg tcgccgccga tgaacacgcc
      481 gtggttcacg tcggcctgct tcagctcgaa atccggattg ccgacgagat gacgaaggtt
      541 ttcgggcgtt ccggtgatga agttgtccag cgcgaggaca cgcatgcctc ggtcgagcag
      601 cgcctcgcac aggtgcgaac cgataaaacc ggctccccca gaaactaccg ctcgctgttt
      661 attcgcggcc aatgccgcgg tacgtgaagc ccaggctccg catcactgcg ggatcgtaga
      721 tgttgcgtcc gtcgacgatg accggccggc gcatcacctg cttcaagcgc gccatgtcga
      781 gccggcggaa ctcgttccac tcggtcacca ggacgacggc gtccgctccc gaggcgacct
      841 cataggcgtc cctgccgtat tcgatctccg gaagcaccct cttcgcggtc tctatagcgg
      901 ccgggtcgta ggcgcgaacc tccgcgccgg cggcgatcag caccttggca atgtcgacgc
      961 tcggcgcctc gcgcatgtcg tccgtgttgg gcttgaaggc gaggccgagg agaccgacga
     1021 cgcgtccagg cagctcgtcg agcagctcgc gcaaaagatc gatgacgacc atgcgctgat
     1081 cgcggttgat ctccatgacc gcatggagca gctgtgggtg gtagttgaat ctctccgaga
     1141 tcgccgccaa cgccgcgaca tcttttggaa aacagctccc gccccagccg atcccggcgt
     1201 cgaggtagtg ctgcccgatc ctcttgtcca ggcccatgcc ttcggcaacg agcttggcgt
     1261 cggcgcccac gcgctcgctg atccaggcga tctcgttgat gaaggagatg cgcgtggcga
     1321 gaaatgcgtt cgacgcgtac ttgatcatct ccgcggtgta gatgtccggc atgaccagga
     1381 tcggcgcatc gagcggcgcg tagagtcccg ccaccttttc cgccgcctgg cggtcgttgc
     1441 tcccgatgac gacgcggtcc gggtgcatga agtcctggat cgccgagcct tcgcgtagaa
     1501 attccgggtt cgacacgaca gccgtccgat gcttcgtatt gccttttgtc aggacgcccg
     1561 agaccatgtc gccggtcaac ggcgggaccg tcgacttgtt gacgacgatc agcggtccct
     1621 ccatcgcgtc ggcgatcgag ctggccgccg actccacgtg cgacagatcg gcctctccgc
     1681 cttcgccttc gggcgtgctc acggcgatga tcgcgaactc ggcgcccggc accgcgtcct
     1741 tatatgaggt cgtgaaggtc agccgccccg cgcgcgcgtt gcgctcgacc agctcccgca
     1801 tgcccggctc gaagaacggc acctggtagc gcttcagcag cgcgatcctg ccctggtcga
     1861 tgtcgaccag ggtgacgtca ttgccaag
//
LOCUS       DATLKL010000335         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_139332, whole genome shotgun sequence.
ACCESSION   DATLKL010000335 DATLKL010000000
VERSION     DATLKL010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139332"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1539
                     /locus_tag="VM713_04380"
     CDS             124..1539
                     /locus_tag="VM713_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83537.1"
                     /translation="MSLAQYVLLALLLCAGAGAAQAGEPPEDKDLDLIPPAAPAAAPR
                     AAAQSAGALQKIYVEDAFTASWLQDSLVPAPAPLPPRWEERALLDARKEWQLADGVRL
                     AYSGRLNLRAAEGLGSPSHENLTNDLREAYLSWEPVDRLFLDAGRINLKSGIALGFNP
                     TDFFKTRAVVEPLSADPAVLREDRLGVLMLRAQRVWDAGSLTAAYAPAVASDSSIYTS
                     FDLPSFNPMFDRTNAHERWLLKGTVDVAGGLSPEVLAYASEGRVRFGANVAENIGKSV
                     VAYLEWSGGRQGSVSSDALAFGRATGSLPAAAAGLLPGLAPTRFRNELAVGASYTTAL
                     PKITFNLEFHYEESGFSRRDWDQWFRTGNGSSAQSPIAGALWYVRDYASDQQRLISRR
                     FAFLNMDWVDAFVPKLEVSGFIDTDLVDGSTRLQLGANYDLSDRWTIGALVLIDSGGR
                     RSNFGSLPQGGSVLLKVVRYF"
     gene            complement(1530..>2064)
                     /locus_tag="VM713_04385"
     CDS             complement(1530..>2064)
                     /locus_tag="VM713_04385"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HVH83538.1"
                     /translation="TSTLEIATRARVSKRELYALVGGKQDMLIACIAERSARMRWVPA
                     ELPVPHDRESLTRALEAFGARLLGEVSHPTVVATFRLAIAEAKRAPEVARALESQGRR
                     VNRAPLNEVLAAASAAGILHGEPAEMAEQFIALLWGDLRMALLLRLAEPPDTSELRRR
                     ARKAAGALLQLHGSDQK"
BASE COUNT          299 a          746 c          699 g          320 t
ORIGIN      
        1 gacgatcatc atcgacgcgg tcgactcatc gctcgttacc accgccagcc tcggcgagta
       61 cgcttaccag gagatcccgg aggcctggtt ccagcgcgat tacctgcccc acgtgcaggc
      121 cagatgagcc tcgcgcagta cgtcctcctc gcactgctgc tctgcgccgg ggcgggcgcg
      181 gcgcaggccg gggagcctcc ggaggacaag gacctggatc tcatcccacc ggctgcgcct
      241 gccgcggcgc cgcgcgcggc ggcgcagagc gccggcgcgc tgcagaagat ctacgtggag
      301 gacgccttca ctgcgtcgtg gctgcaggac tccctcgtgc cggcgccggc gccgttgccg
      361 ccgcgctggg aggagcgggc actcctcgat gcgcgcaagg agtggcagct cgccgacggc
      421 gtgcgtctcg cctacagcgg ccggctgaac ctgcgcgccg cggaggggct cgggtccccg
      481 agccacgaga acctgaccaa cgatctgcgc gaggcctacc tgagctggga gcccgtcgac
      541 cggctgttcc tcgatgccgg acgcatcaat ctcaagagcg ggatcgcgct cggcttcaat
      601 cccacagact tcttcaagac gcgcgccgtg gtcgagccgc tctccgcgga cccggcggtg
      661 ctgcgcgagg accgcctcgg agtgctgatg ttgcgcgcac agcgcgtttg ggatgcgggc
      721 tccctgaccg ccgcctacgc gccggcggtg gcctccgaca gcagcatcta cacctcgttc
      781 gacctgccga gcttcaatcc gatgttcgac cgaaccaacg cgcacgaacg ctggctgttg
      841 aagggcactg tcgacgtcgc gggaggactg agccccgagg ttcttgccta tgcctccgag
      901 ggacgcgtgc gcttcggcgc aaacgtcgcc gagaacatcg gcaagagcgt cgtcgcctac
      961 ctcgagtggt ccggcggccg ccagggcagc gtgagctcgg acgccctcgc tttcggccgc
     1021 gcgaccggca gcctgccggc ggccgccgcg ggcctgcttc ccggcctcgc gccgacgcgc
     1081 tttcgcaacg agctcgccgt cggcgcctcg tacacgaccg cgctgccgaa gatcaccttc
     1141 aacctcgagt tccactacga ggagtccggg ttctcgcgcc gcgactggga tcagtggttc
     1201 cgcaccggca acggctcgag cgcacagtcg ccgatcgcgg gcgcgctctg gtacgtcaga
     1261 gactatgcgt ccgaccagca aaggctgatc agccgccgct tcgcgttcct gaacatggac
     1321 tgggtcgatg ccttcgtgcc caagctcgaa gtgtcgggct tcatcgatac cgacttggtc
     1381 gacggctcga cccggctgca actcggcgcg aactacgacc tgtcggaccg ctggacgatc
     1441 ggcgccctgg tcctcatcga ctcggggggc cgtcgttcca acttcggcag cttgccgcag
     1501 ggcggcagcg tgctccttaa ggtcgtgcgc tacttctgat ccgacccgtg gagctgcagg
     1561 agagccccgg ccgctttgcg ggcgcggcgc ctgagctcgg acgtgtcggg aggctcggcg
     1621 aggcgcagca gcagagccat gcgcagatcg ccccagagga gcgcgatgaa ctgctcggcc
     1681 atctccgccg gctccccgtg caggattccc gccgcgctgg cggcggccag cacctcattc
     1741 agcggggcac gattcacccg gcggccctgc gactcgagcg cgcgtgccac ttctggcgcc
     1801 cgtttggcct cggcgatggc gagccggaag gtcgccacga ccgtcggatg gctgacctcg
     1861 ccgagcagcc gcgcgccgaa ggcctcgagc gcgcgcgtga gactctcgcg gtcgtgcggc
     1921 accggcagct ccgccggcac ccagcgcatc cgcgccgagc gctcagcgat gcaggcgatc
     1981 agcatgtcct gcttgccgcc gaccagggcg tagagctcgc gcttcgagac cctggcgcgc
     2041 gtcgcgatct cgagcgtgct ggtc
//
LOCUS       DATLKL010000336         3364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_60235, whole genome shotgun sequence.
ACCESSION   DATLKL010000336 DATLKL010000000
VERSION     DATLKL010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60235"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VM713_04390"
     CDS             complement(<1..371)
                     /locus_tag="VM713_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83539.1"
                     /translation="MNEAAEACRWRTSLIERADTSAGWRYRKAREFPWDARNVESAKS
                     LRRLHAALTDLRPDHDLWSRYAAICSRAAGEECLRLVEIEYAMLHSYGFSIHGAAGSA
                     EGFLLELLPALELELAAPAHDG"
     gene            646..1890
                     /locus_tag="VM713_04395"
     CDS             646..1890
                     /locus_tag="VM713_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVH83540.1"
                     /translation="MNSPSDEPKPVTWIGRDRPFERAPVEPVPVTKMTPAKTLLDRRW
                     WIIGALVGIGLVALLAWRLMAGGPKPATSLADRGGVPLVSVIVPGQQAVTASVSITGA
                     IAARYDMPIANEGDTGRIVGVYVEAGDHVKRGQTLAKVDDSVIVQQVNRLEASLEQAR
                     AQAELSAAEYKRALGVQAAGALSAEDIEKRRATSITDAANVKVVAAQLAEAQARLART
                     RITAPVEGTVLTRHAEVGQIANPGGEALFRVASGGEVEMRGQIAEQDLAQVRTGQTAT
                     VHLTGVDRPFTGQVRLLGAIIDPATRLGDIRIQLKPDPELRPGAFARAEVTINQAQRP
                     VLPQSAVMADTNGSYVLVVGAADKVERRSVKVSGTTDNGVIIAEGLTGNERVVSAAGG
                     FLRDGETVKVAPAPGPAAGGAT"
     gene            1905..>3364
                     /locus_tag="VM713_04400"
     CDS             1905..>3364
                     /locus_tag="VM713_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH83541.1"
                     /translation="MKNISAWAIRHPVSPVVLFVVLLFLGTVAFNRLPRTLNPDVTFP
                     VVLVQVFQPGASPQEIETQIMQKVEAAVAGVGNIDNIISLAIEGQSRVFVQFNIGTPI
                     DRAVADVRDAVAKVRVDLPQGIQEPTVTRIDIDGNEIVAYAVSSSALSAEDLSWFVDN
                     AVSKSLLAVEGVSQVTRGGGVSREIRVELDPARMQALGITAAQVNQQLRTLNLDSPGG
                     RAEIGGGEQAIRVLGEARTARALGDTRIMLPGGRFARLSDLADVKDSVGEIRALARLN
                     GRPATIFKVFKAKGTSDVNVLAGVEKELAKIEKQNPQVTISQVYTSVDYTKATYHSAM
                     SALIEGSILAVLVVFLFLRDVRATLIAALAIPLSAIPTFALMQWVDFTLNQISLLALS
                     LVAGVLVDDAIVEIENIVRHMRMGKSGYQAALDAADQIGLAVVATSATIIAVFLPVSF
                     MGGLTGQYFKQFGLTVAAAVFFSLLVARLITPVLAA"
BASE COUNT          540 a         1161 c         1108 g          555 t
ORIGIN      
        1 ccgtcgtgcg caggcgctgc cagctcgagc tcgagggccg ggagaagctc gagcagaaac
       61 ccttccgcgc ttcccgctgc gccgtgaatc gagaacccgt acgagtgcag cattgcgtat
      121 tcgatctcga ccaaccgcag gcactcttct cccgccgccc ggctgcagat cgcggcgtac
      181 ctgctccata agtcgtggtc ggggcgcaga tcggtcagcg ccgcgtgcag ccgccgcagc
      241 gacttggcgc tttcgacatt ccgtgcgtcc caggggaatt cgcgcgcctt gcgatatctc
      301 catcccgcag aggtatcagc cctctcgatc aggctggtgc gccagcggca agcctcggca
      361 gcctcattca tgtgcccctc cttccatggc gccgtcagat cgtgagcttc ccgcgcggcc
      421 gaccggcccg ccacctgtct ccggttagca acggggtcct gctatcctcc gcacccctcg
      481 gcccgtcgcc cgcgcatgtc ccttgggcgc gttgggggcg ggaattaccg ttggttacga
      541 ttaaacttcg ctgcaacgcg ctggttctaa agggtatcgg cgtggtctcg agcgccgtag
      601 gcacggattt gacaaggaat ccccgtgggc acaaacccgg aacatatgaa cagcccctcc
      661 gatgagccca agccggtcac ctggatcggc cgcgaccgcc cgttcgagcg cgcgcccgtc
      721 gagcccgttc ccgtcacgaa aatgacgccc gccaagacgc tgctcgaccg gcgctggtgg
      781 atcatcggtg ccctggtcgg catcggcctg gtggcgctgc tcgcctggcg tctcatggcc
      841 ggcggcccga agcccgcaac ctccctcgcc gaccgcggcg gagtgccgct cgtgagcgtg
      901 atcgtcccgg ggcagcaggc agttacggcc agtgtgagca tcacaggcgc cattgcagcc
      961 cgctacgaca tgccgatcgc caacgagggc gacaccggcc gtatcgtcgg cgtctacgtc
     1021 gaggccggcg atcacgtcaa gcgcgggcag acgctcgcga aagtcgacga ctcggtcatc
     1081 gtgcagcagg tgaatcgact cgaggcctcg ctcgagcagg cgcgagccca ggcagagctg
     1141 tcggccgctg aatacaagcg ggcgctgggc gtgcaggccg ctggcgcgct gtccgccgag
     1201 gacatcgaga agcgtcgcgc gacttcgatc accgacgcgg ccaatgtcaa ggtggtcgcc
     1261 gcgcagctcg cggaagcgca ggcgcggctc gcgcgtacgc gcattactgc gcccgtcgag
     1321 ggcacggtgc tcacgcgcca tgccgaggtg ggccagattg ccaacccggg cggcgaggcg
     1381 ctgttccgcg tcgccagcgg cggcgaggtc gagatgcgcg gacagatcgc cgagcaggat
     1441 ctggcgcagg taagaaccgg gcaaaccgcg acggtgcacc tgaccggcgt ggatcgcccc
     1501 ttcaccggcc aggtgcgcct actcggggcg atcatcgacc cggcgacgcg cctcggcgac
     1561 atccggattc agctcaagcc tgatccggag ctgcgtcccg gggcctttgc ccgcgccgag
     1621 gtcacgatca accaagcgca gcgaccggtg ttgccacagt ccgccgtcat ggccgatacc
     1681 aacgggagct acgtgctcgt ggtgggtgca gctgacaaag tcgagcggcg gtcggtcaaa
     1741 gtctccggta cgacggacaa cggcgtgatc atcgccgagg gtctcaccgg gaacgagcgc
     1801 gtcgtgtccg ccgcgggcgg cttcctgcgc gatggcgaga ctgtcaaggt ggcccccgct
     1861 cccggcccgg ctgccggcgg cgccacctga agagtgacgc gcgcatgaaa aacatctcag
     1921 cgtgggcgat ccgccacccg gtgtcgccgg tcgtgctgtt cgtcgtgctg ctgttcctcg
     1981 ggacggtggc cttcaatcgc ctgccgcgca cgctcaaccc cgatgtgacc ttcccggtgg
     2041 tgctggtgca ggtgttccag cccggcgcgt cgccccagga gatcgagacc cagatcatgc
     2101 agaaggtcga ggccgccgtc gccggggtgg gcaacatcga caacatcatc tcgctcgcga
     2161 tcgagggcca gtcgcgcgtc ttcgttcaat tcaacatcgg cactcccatc gatcgcgcgg
     2221 tggcggacgt gcgcgacgcg gtagccaaag tgcgcgtcga cctgccgcag ggcattcagg
     2281 agcccaccgt cacccgcatc gacatcgacg gcaacgagat tgtcgcctac gcggtgagct
     2341 cgagtgcact ctcggccgaa gacctgtcct ggttcgtcga caatgccgtc agcaagagcc
     2401 tgctcgcggt cgaaggcgtg tcgcaggtga cccggggcgg cggcgtcagc cgcgagatcc
     2461 gggtcgagct cgatccggca cgcatgcagg cgctcggcat caccgccgcg caggtcaatc
     2521 agcagttgcg taccttgaac ctcgactccc ccggggggcg cgccgagatc ggtggcggcg
     2581 agcaggcgat ccgcgtgctg ggcgaggccc gcacggcacg cgcgctcggt gacacccgga
     2641 tcatgctgcc aggcgggcgg ttcgcacgtc tctcggacct ggccgacgtc aaggacagcg
     2701 tcggcgagat ccgcgcgctc gcccgcctga acgggcgccc ggccaccatc ttcaaggtct
     2761 tcaaggccaa gggcacctcc gatgtgaacg tcctcgcggg cgtcgagaag gagctcgcca
     2821 agatcgagaa gcagaacccg caggtcacga tctcgcaggt ctacacctcg gtcgactaca
     2881 ccaaggccac ttatcactcc gcgatgagtg ccctcattga gggctcgatc cttgcggtgc
     2941 tggtggtctt tctgttcctg cgtgacgtcc gcgcaactct catcgcggcg ctcgccatcc
     3001 cgctgtcggc gattccgacc ttcgcgctca tgcagtgggt ggacttcacg ctgaatcaaa
     3061 tcagtctcct cgccctgtcg ctggtcgccg gcgtgctggt ggacgatgcc atcgtcgaga
     3121 tcgagaacat cgtgcgccac atgcgcatgg gcaagagcgg ctaccaggcg gcgctcgatg
     3181 ccgcggatca gatcggactc gcggtcgtgg cgacctccgc gaccatcatc gcggtgttcc
     3241 tgccggtgag cttcatgggc gggctcaccg gacagtactt caagcagttc ggcctcacgg
     3301 tcgcggccgc ggtgttcttc tcgctgctgg tggcgcgcct catcaccccg gtgctcgccg
     3361 ccta
//
LOCUS       DATLKL010000337         3671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_195537, whole genome shotgun sequence.
ACCESSION   DATLKL010000337 DATLKL010000000
VERSION     DATLKL010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195537"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VM713_04405"
     CDS             complement(<1..501)
                     /locus_tag="VM713_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVH83542.1"
                     /translation="MSAASRTVGRYVIETLAANGIDTAFGIPGVHNIELYRGFESRRV
                     RHVLARHEQNAAFAADGFARMSGRAAAAFVISGPGLTNAITAIAQAASDSVPMLVIAS
                     TPVRASLGRHWGVLHELADQRGVAAGAAGLALHASSAAEVRAALAAAFAFLSGGRPRP
                     AYVGIPL"
     gene            542..1732
                     /locus_tag="VM713_04410"
     CDS             542..1732
                     /locus_tag="VM713_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HVH83543.1"
                     /translation="MKFSSLVGRVGGTGADAWLTHHQAAETRARGEDVIVLSVGDPDI
                     DSPAPVLERAIEALRRGDTHYVAPAGRPELRQAIARTHAARTGQAVSAENVVYLAGAQ
                     NALFVASLCLAGPGDEVVSFEPMYPTYPATLEVGGARLVHAPAGEGLRPDLAALAALV
                     TSRTRAIVWASPNNPSGIIFTAAELAVIGELARRHDLWLVADEVYAGLAPGGRVPGLA
                     ALLPEQVVTLGSLSKSHAMTGWRAGWLVGPRELAAKAELLAMCMLFGLPGFVQEAAIT
                     ALAIAPEAEQRTREYCRTRQLRFAAGLRGVRELRLIEPEAGMFMLIDVSATGLSGREF
                     VRALYAAQKVSLLDGAAFGAATAGCVRACFATDEASIDSACERIRRFCATGLAAVRPA
                     SAAG"
     gene            complement(1718..3202)
                     /locus_tag="VM713_04415"
     CDS             complement(1718..3202)
                     /locus_tag="VM713_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVH83544.1"
                     /translation="MARARGLAVLRRKSVALCLAESRGERGPHLERALGFWSLTAFGV
                     GCTIGAGIFSLTGEVAAHQAGPAVSLAFVLASLACFFAGLCYAEFAAMVPISGSAYSY
                     AYVTLGELTAWMIGWSLVLEWLFSASLVAISWSGYAQAALHDLGLVLPPRFAQAPFAV
                     DAHHHLLATGAWLDLPAVTVVLACTALTLAGTRTSAAVNTAIVLAKVFALLVLVVVGA
                     HYVHPAHWQPFIPANDGGFGHFGWSGLARGAGILFFAYLGFDGVSTLAEEARNPQRTL
                     PWSLFASLLICTVLYVSVSLVITGLADYRSLAVADPLYYALSAARVPLGSVRGLVSVV
                     ALVGLVSVVLACIVGQVRIFYSMGRDGLLPAAFARVSRAHRTPVIGTVLTGAVAAVIA
                     GVIPLGTLGELISLGTLLAFTMVCVGVLVLRRIDPAAHRPFRTPAVPLVPLLGIASCA
                     VLMVSLPGGTWLQLCAWSTLGLVIYAAYGRRHSRLAAAAPSAGR"
     gene            complement(3206..3538)
                     /locus_tag="VM713_04420"
     CDS             complement(3206..3538)
                     /locus_tag="VM713_04420"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HVH83545.1"
                     /translation="MGLLDGLIGGVVGAEMASVVNTFIQQHGGVQGIVSQLESQGLGA
                     TARSWVSTGANQSISPAEISKVFGSSPVLAQLAAKAGMSPQDLAQKLAQVLPAAVDKL
                     TPGGAIAK"
BASE COUNT          591 a         1262 c         1338 g          480 t
ORIGIN      
        1 gagagggata ccgacatagg cgggcctggg ccgcccgccg cttaggaatg caaacgccgc
       61 ggcgagcgcg gcgcgcacct ccgccgcaga gctcgcatgg agggccagcc ctgcggcgcc
      121 ggccgccacg ccccgctggt cggcgagctc atgcagcaca ccccagtgac ggccgagcga
      181 ggcgcgcacg ggcgtgctgg cgatcaccag catcggcacc gaatcggacg cagcctgcgc
      241 aatggccgtg atggcgttgg tgagccctgg acccgagatc acgaatgccg cggcggcgcg
      301 cccgctcatg cgggcaaagc cgtcggctgc gaaggccgcg ttctgctcgt ggcgggcgag
      361 cacgtggcgc acgcgccgcg actcgaagcc gcgatatagc tcgatgttgt gtacgccggg
      421 gatgccgaag gcggtgtcga tgccgttggc cgcgagcgtc tcgatgacat agcgtccgac
      481 tgtgcgggaa gctgcgctca tgcgcttgaa atgttagcac cgcccacgca gagtaagctg
      541 cgtgaagttc tcctccctcg taggaagggt cggcggcact ggcgccgacg cctggctcac
      601 gcaccatcag gcggccgaga cgcgcgcgcg cggcgaggat gtgatcgtgc tgagtgtcgg
      661 cgaccccgac atcgatagcc ccgcgccggt gctcgagcgc gccatcgaag cgctgcgccg
      721 cggcgacacg cactacgtgg cgccggccgg ccggcccgag ctgcggcagg cgatcgcgcg
      781 cacgcacgca gcgcgtaccg ggcaggcggt ctccgccgag aacgtcgtct accttgccgg
      841 agcgcagaac gcgctattcg tcgcctcgct gtgcctcgcc ggccccgggg atgaggtcgt
      901 gagcttcgaa cccatgtacc cgacctaccc ggcgaccctg gaagtgggcg gcgcgcggct
      961 ggtgcatgcg ccggcgggtg aggggctgcg tccggacctc gcggcgcttg cggcgctcgt
     1021 cacgagccgc acgcgcgcca tcgtctgggc gagccccaac aacccgagcg gcatcatctt
     1081 caccgcggcc gagctcgccg tcatcggcga gctggcgcgc cgccacgatc tgtggctggt
     1141 cgcagacgag gtctacgcag gactcgcccc tggcggccgc gtgccggggc tcgccgcgct
     1201 cctcccggag caggtcgtga cgctcgggag tctctcgaag tcgcatgcca tgacgggctg
     1261 gcgcgccggc tggctcgtcg gtccgcgcga gctcgcggcg aaggccgagc tcctcgccat
     1321 gtgcatgctg ttcgggctgc cgggcttcgt gcaggaggcg gcgatcaccg cgctcgccat
     1381 cgcgcccgag gccgagcagc gcacccgcga gtactgccgc acgcgtcagc tgcgcttcgc
     1441 ggccggattg cgcggcgtgc gcgagttgcg cctcatcgag cccgaggctg gcatgttcat
     1501 gctgatcgac gtgagcgcca ccggcctctc gggccgtgaa ttcgtgcgcg cgctctacgc
     1561 cgcgcagaag gtgtcgctgc tggacggcgc ggctttcggg gcggcgaccg ccggctgcgt
     1621 gcgggcgtgc ttcgcgaccg acgaagcgag catcgatagc gcctgcgagc gcatccgccg
     1681 cttctgcgcg accgggctcg ccgccgtgcg cccggcctca gcggccggct gacggcgccg
     1741 cggcggcgag gcgcgaatga cgccgtccgt aggcggcgta gatgacgagc ccgagcgtgc
     1801 tccacgcgca cagctgcagc caggttccac ccggcaggct caccatcagg accgcgcagc
     1861 tggcgatgcc gagcagcggt acgagcggca ccgcgggcgt gcggaaggga cggtgcgcgg
     1921 cgggatcgat ccggcgcagc accagcactc cgacgcacac catagtgaag gcgagcagcg
     1981 tgccgagcga gatgagctcg cccagcgtcc cgagcgggat gacaccggcg atcactgcgg
     2041 cgacggcgcc cgtcagcacc gtgccgatga ccggtgtccg atgggcgcgg ctcacgcgcg
     2101 cgaatgccgc cggcagaaga ccgtcgcgcc ccattgagta aaagatccgc acctgtccga
     2161 cgatgcaggc cagcacgacg gatacgaggc cgacgagcgc gacgacgctc acgagccccc
     2221 tgaccgagcc gagcgggacg cgggccgccg acagcgcgta gtagagcgga tcggcgacgg
     2281 cgagcgagcg gtagtcggcg agaccggtga tgacgaggct gacgctgaca tacagcacgg
     2341 tgcagatgag gagtgaggcg aacaggctcc aggggagggt gcgctgcgga ttgcgggcct
     2401 cctccgcgag cgtcgagacg ccgtcgaagc cgaggtaggc gaagaacagg atcccggcgc
     2461 cccgcgccag ccccgaccag ccaaagtgtc cgaagccacc gtcgtttgcc gggatgaagg
     2521 gctgccagtg agccggatgc acgtagtgcg cacccacgac caccagcacg aggagcgcga
     2581 agactttcgc cagcacgatc gcggtgttga ccgcggccga ggtgcgcgtg ccggcgagcg
     2641 tgagagccgt gcaggccagc accaccgtga ccgcgggcag gtccaaccag gcgccggtcg
     2701 cgagcagatg gtgatgggcg tcgaccgcga agggcgcctg cgcaaaccgc ggcggcagca
     2761 cgagaccgag gtcgtgcagc gccgcctgcg cgtaccccga ccagctgatc gccaccagcg
     2821 acgccgagaa cagccactcg agcaccagac tccagccgat catccaggcg gtgagctcac
     2881 cgagtgtcac gtaggcgtac gagtaggcgc tgcccgagat tggcaccatc gcggcgaatt
     2941 ccgcgtagca cagtcctgcg aagaagcagg cgagactggc gagcacgaag gcgagcgata
     3001 ccgcgggccc cgcctgatgg gcggcgacct cgccggtgag cgagaagatg cccgcgccga
     3061 tggtgcagcc gacgccgaag gccgtcaggc tccagaagcc gagggcgcgt tcgagatgcg
     3121 gaccgcgttc gccgcggctc tcggcgaggc acagcgcgac ggacttgcgc cgcaggaccg
     3181 caaggccgcg ggcgcgggcc acgggttact tggcgatagc tcctccgggg gtgagcttgt
     3241 cgacggcggc ggggagcacc tgcgcgagct tctgcgccag atcctgcggg ctcatgcccg
     3301 ccttggcggc gagctgggcg aggaccgggc tgctgccgaa gaccttgctg atctcggcgg
     3361 gcgagatcga ctggttcgca ccggtggaaa cccaggagcg cgccgtagca ccgagcccct
     3421 ggctttcgag ctggctcacg atcccctgca ccccgccgtg ctgctggatg aaggtgttca
     3481 ccacgctcgc catctcggcg ccgacgacgc cgccaatcaa tccatcgagc aatcccatga
     3541 tgcctcccgt gggtgagctg agccgttgtg gcgcgcattg tgcgcgcgct cccccggtgc
     3601 gtgcaacgcc gccttgttat attattacga cgacattaaa aagagcgcgc cggtgtcggg
     3661 cgcgctggat g
//
LOCUS       DATLKL010000338         1747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_139368, whole genome shotgun sequence.
ACCESSION   DATLKL010000338 DATLKL010000000
VERSION     DATLKL010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139368"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..903)
                     /locus_tag="VM713_04425"
     CDS             complement(124..903)
                     /locus_tag="VM713_04425"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HVH83546.1"
                     /translation="MCLLVLAWAAHPRYRLIVAANRDEFHERPAAPLAKWPEPPHILA
                     GRDLRASGTWLGLDPARRFGVVTNFRELQGREPAAPSRGRLIPQYLAGADRPGQFFAR
                     LEPAAKEYSGFNLLLTDAASLWYGSNRAAPFARELPPGVYGLSNELLDTPWPKLVRVK
                     ARFQQWLAQPDATAAGLFALLADRTEAAEADGLPTGGLPREWERILSAPFVAHPTYGT
                     RCTTVLLLEAGGALYLAERRFDAAGEPAGESEFRLNAGDWP"
     gene            complement(910..>1747)
                     /gene="nudC"
                     /locus_tag="VM713_04430"
     CDS             complement(910..>1747)
                     /gene="nudC"
                     /locus_tag="VM713_04430"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HVH83547.1"
                     /translation="EAREDPEWVAAAWADGATVYVVVRGTAHLLHGDPPRAALLTRAE
                     PLVRSAPATSLTLLGWFHGARCVLITLPPERAAPPPGMRFEELRPLSALLPADEAGLL
                     AYARALAVWRARTRHCGLCGAPTAPIRAGHCLVCSNHACAEEFFPRIDPAIIVLVSDG
                     ERALLGRQASWPARRYSTIAGFVEPGESLEDAVAREVEEETGVRIVSAGYDSSQPWPF
                     PSSLMLGFRAQAPAGSAVRVSGELEDARWFTRAQLTSGEALAPPSHSISWRLISRWLD
                     GG"
BASE COUNT          248 a          632 c          660 g          207 t
ORIGIN      
        1 catctcgacg ttggatcggg aagattcacc ggattctaga gcctcgcgag cgcactcgct
       61 cgccgctctt cacagtcgga gcgcgcgggc tgcgcgtcgg ttccgcgcgg cggtccgcat
      121 gacttaaggc cagtcgccgg cgttcagccg gaattcactt tcgccggcgg gctcgcccgc
      181 ggcgtcgaag cgccgctcgg cgagatacag cgcccccccc gcctcgagca gcagcaccgt
      241 cgtgcagcgc gtgccgtagg tcgggtgagc cacgaagggc gccgacagga tccgctccca
      301 ctcgcgcggc aggccgccgg tcggcagccc gtcggcctcc gccgcctcgg tgcgatccgc
      361 gagcagcgcg aacaagccgg ccgccgtggc gtccggctgc gcgagccatt gttgaaagcg
      421 cgccttgacg cgcaccagct tcggccacgg cgtgtcgagc agctcgttcg agaggccgta
      481 gacgccggga ggcagctcgc gcgcgaacgg cgcggcgcgg ttcgagccgt accagagcga
      541 tgccgcgtcg gtcagcagca ggttgaaccc ggaatactcc ttggccgcgg gctcgagccg
      601 cgcgaagaac tggccggggc ggtccgcgcc cgcgaggtat tgcgggatca ggcggccgcg
      661 cgacggggcc gccggctcgc gtccctgcag ctcgcggaag ttggtgacca caccgaagcg
      721 gcgcgccggg tcgagcccga gccaggtgcc tgaagcgcgc agatcgcggc cggcgaggat
      781 gtggggcggc tccggccatt tggcgagcgg cgccgccggc cgctcgtgga actcgtcacg
      841 gttggcggcg acgatgaggc ggtagcgcgg gtgcgctgcc caggcaagca ccagcaggca
      901 cacgcgcgcc tacccgccgt cgagccagcg tgagatcagg cgccaggaga tcgagtgcga
      961 gggcggcgcg agcgcctcgc ccgaggtgag ctgcgcgcgc gtgaaccagc gcgcgtcctc
     1021 gagctcgccg gagacgcgca cggcgctgcc cgcgggcgcc tgcgcccgga acccgagcat
     1081 gagcgaggag gggaacggcc agggctgcga ggagtcgtag cccgcgctca cgatgcgtac
     1141 gccggtttcc tcctcgacct cgcgcgcgac cgcgtcctca aggctctcgc ccggctcgac
     1201 gaagccggca atggtcgagt agcgccgcgc cggccagctc gcctgccgcc cgagcagcgc
     1261 ccgctccccg tcgctcacca gcacgatgat ggccggatcg atgcgtggaa agaactcctc
     1321 cgcgcaggcg tggttgctgc acaccagaca gtgcccggcg cggatcggcg ccgtgggcgc
     1381 accgcacagg ccgcagtggc gcgtgcgtgc gcgccacacc gcgagcgcgc gcgcgtaggc
     1441 gagcagcccc gcctcgtccg cgggcagtag cgccgagagc ggccgcagct cctcaaagcg
     1501 catgccgggg ggcggcgcgg cgcgctccgg cggcagagtg atcagcacgc agcgcgcgcc
     1561 gtggaaccag ccgaggagcg tcaggctcgt ggccggtgcc gagcgcacca gcggctcagc
     1621 gcgggtgagc agcgccgcgc gcggtggatc gccgtggagc aggtgggcgg tgccgcgcac
     1681 caccacatag acggtggcgc cgtcggccca ggccgccgcg acccattcgg ggtcctcgcg
     1741 cgcttct
//
LOCUS       DATLKL010000339         1606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_161704, whole genome shotgun sequence.
ACCESSION   DATLKL010000339 DATLKL010000000
VERSION     DATLKL010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161704"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..369)
                     /locus_tag="VM713_04435"
     CDS             complement(91..369)
                     /locus_tag="VM713_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83548.1"
                     /translation="MYETTDSEFADSALAALGAAEIPCYRVGRSCADGVQSAVNIYIE
                     RDSDYAEANRILISLGAAVERQPPAWLIGFIMLVAALGALGIALGGSS"
     gene            complement(385..906)
                     /locus_tag="VM713_04440"
     CDS             complement(385..906)
                     /locus_tag="VM713_04440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVH83549.1"
                     /translation="MSPSGTDRVVTVRRAAAADVPLILAFIRELAVYEHLEHEVVATE
                     ADVHAALFGPLAYAEVALACLDGQPVGFALYFHNFSTFIGKPGIYLEDLFVRPEARGL
                     GAGKRLFAYLAKTALERGCARLDWAVLDWNEPSIGFYRSLGAVDQSEWTTYRLDGAAL
                     ARLAESYRSALKE"
     gene            complement(912..1583)
                     /locus_tag="VM713_04445"
     CDS             complement(912..1583)
                     /locus_tag="VM713_04445"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HVH83550.1"
                     /translation="MRRAARRREPRGLPAARVPGRRLLRILPRDHSDRRDRAHADRLA
                     LDSSRRGGGIENLLPVPWVFAWSQTRYMLPEWYGAGAGLGAALDRFGLPRLRAVYGEW
                     FFMRNLLDDIETMLARVDLDIAQHYDALAPEPLRRFAGSIRAEYLAACQRILAIKDSR
                     ALLDSDPTMQRSIRLRNPYVDPMNLMQVDLLKRWRARGREDRDLFEALLASVGGIAQG
                     LQASG"
BASE COUNT          271 a          537 c          567 g          231 t
ORIGIN      
        1 ggacgccgag cgggttgtcg aggctctggt cagggcccgc tgcatagccg tcgagggaaa
       61 tcgcgaagct gcggacgagg agcctcgaca ctagctcgac cctcccaagg caatacctaa
      121 ggcaccgagc gccgccacca gcatgatgaa gccgatcagc cacgcgggcg gctgccgctc
      181 tacggccgca ccgaggctga tgaggattct attcgcctcg gcgtagtcgc tgtcgcgctc
      241 gatgtagatg ttgaccgcgc tctggacacc gtcggcgcag ctgcgaccga cccggtagca
      301 gggaatttcc gccgcgccga gtgccgcgag cgcgctgtcg gcgaactcgg agtcggtggt
      361 ctcgtacacg agccgtgcca ggactcactc cttgagcgct gagcggtaac tctcggccag
      421 ccgcgccagc gccgcaccat ccaggcggta ggtggtccac tcgctctgat cgacggcccc
      481 gaggctgcgg tagaaaccga tggagggctc gttccagtcg agcaccgccc agtcgagccg
      541 tgcacagccg cgctcgagtg cggtcttcgc caggtacgcg aacaggcgct tgccggcgcc
      601 gaggccgcgc gcttcggggc gcacaaacag atcctcgaga tagatcccgg gcttgccgat
      661 gaaagtcgag aagttatgga agtacagggc aaagccgacc ggctgcccat cgaggcaggc
      721 cagcgcgacc tctgcataag ccagcggccc gaacagcgcc gcgtgcacgt cggcctccgt
      781 ggccaccacc tcgtgctcga ggtgctcgta gacggcgagc tcgcggatga aggccagaat
      841 gagcggcacg tcggccgcag cggcgcgacg gaccgtgacg acgcgatcgg tgcctgacgg
      901 gctcacgtgg atcagccgct cgcctgcagc ccctgtgcga tgccgccgac gctcgccaac
      961 agcgcctcga acagatcgcg gtcctcgcgg ccgcgtgcgc gccagcgctt gagcaggtcc
     1021 acctgcatca ggttcatggg gtcgacgtac gggttgcgca gccggatcga gcgctgcatg
     1081 gtcggatccg agtcgagcag cgcgcggctg tccttgatcg cgaggatccg ctggcaggcc
     1141 gcgaggtatt ccgcgcggat gctgccggcg aagcggcgca gcggctcggg ggcgagcgcg
     1201 tcgtagtgct gcgcgatgtc gagatcgacg cgcgccagca tggtctcgat gtcgtcgagc
     1261 aggttcctca tgaagaacca ctcgccatag acggccctga ggcgcggcag gccgaagcga
     1321 tcgagagcgg cgccgaggcc ggcgccggcg ccgtaccact cgggcagcat gtagcgcgtc
     1381 tgcgaccagg cgaacaccca cggcacgggc agcaggttct cgatcccgcc ccctcggcgc
     1441 gatgagtcga gcgcgagccg atctgcatgc gctcgatcac gtcgatcgga gtgatcgcgc
     1501 ggaagtattc gtagaagtcg gcgtcctggt acacgagccg ccggtaggcc gcgcggctct
     1561 cggcggcgag cagcgcggcg cactcgaggc gccccgggtc gtcggg
//
LOCUS       DATLKL010000340         1627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_116757, whole genome shotgun sequence.
ACCESSION   DATLKL010000340 DATLKL010000000
VERSION     DATLKL010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116757"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VM713_04450"
     CDS             complement(<1..248)
                     /locus_tag="VM713_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83551.1"
                     /translation="MRNVGIIMRRELASYFATPLAYVFILIFLVLGNAFTFYLGGFYE
                     RGQADLDSLFGFLPWLFLFLIPALSMKLWAEERNSGSI"
     gene            complement(263..1186)
                     /locus_tag="VM713_04455"
     CDS             complement(263..1186)
                     /locus_tag="VM713_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVH83552.1"
                     /translation="MIKTEHLTKRYGPLMAADDVTFEVGPGEVLGFLGPNGAGKTTTM
                     RMLAGFVTPTSGSGSICGHDIGSDTLAAKRVLGYLPEGAPSYGEMTVRAFLAFIADLR
                     ALEGERRGARLAHVIEHLHLGGVLEQTIDTLSKGFRRRVGLAQAIVHDPPVLILDEPT
                     DGLDPNQKHEVRTLINDMARDKIIVISTHILEEVDAVCTRAIIIARGRIVADDTPAGL
                     AARSRYHNAVSMQLDDPTQLTAARAAVASLPTVADLEVSERDARLTALPKAGATILPA
                     LSELAVRQGLKLKELHLESGRLDEVFRTITA"
BASE COUNT          260 a          508 c          575 g          284 t
ORIGIN      
        1 tcgatgctgc cggagttgcg ttcctcggcc cacagcttca tcgacaaggc tggaatcagg
       61 aacaggaaca gccaggggag aaagccgaag agcgagtcga gatccgcctg gccgcgctcg
      121 tagaagccgc ccagatagaa cgtgaaggca ttgcccagca cgagaaagat gagtatgaaa
      181 acgtacgcga gcggcgtcgc gaagtaactc gccagctcgc ggcgcatgat gatgcccacg
      241 ttgcgcatgt gcttcccggt ggtcaggcgg taatggtgcg gaatacttcg tcgaggcggc
      301 cggactcgag gtgcagctcc ttgagcttga gtccctgccg cacggcgagc tcggagagcg
      361 ccggcaggat ggtggcaccg gccttcggta aggccgtgag tcgcgcgtcg cgctcgctca
      421 cctcgaggtc ggccaccgtc gggagcgagg cgaccgcggc gcgcgccgcg gtcagctgcg
      481 tcgggtcgtc gagctgcatc gacacggcgt tgtgatagcg cgagcgcgcc gcgaggccgg
      541 cgggcgtgtc gtccgcgacg atgcgcccgc gcgcgatgat gatggcgcgc gtacacacgg
      601 cgtcgacctc ctcgaggatg tgagtcgaga tcacgatgat cttgtcgcgc gccatgtcgt
      661 tgatgagcgt gcgcacctcg tgcttctggt tgggatcgag gccgtcggtg ggctcatcga
      721 gaatgaggac cggaggatcg tgcacgatcg cctgcgcgag cccgacgcgc cgccggaatc
      781 ccttcgacag ggtgtcgatc gtctgctcga ggacgccgcc gaggtgcagg tgctcgatga
      841 cgtgcgcgag ccgcgcgccg cggcgctcgc cctcgagcgc acgcaggtcg gcgatgaagg
      901 cgaggaaggc gcgcacggtc atctcgccgt agctcggcgc cccttccggg agatagccga
      961 ggactcgctt ggcggcgagc gtgtcgctgc cgatgtcgtg tccgcagatg ctgccgctcc
     1021 cggaggtcgg cgtgacgaag ccggcgagca tgcgcatggt ggtggtcttg ccggcgccgt
     1081 tgggcccgag gaagccgagc acctcgcccg gtcccacctc aaaggtcacg tcgtcggcgg
     1141 ccatcagtgg gccgtagcgc ttggtgagat gttcggtctt gatcatgtcg gatgcggtct
     1201 agaggagcag cttcccggga aagttccacg tcattttttg agcgcgcgat tgtgacggga
     1261 gctgcggcgg atttccaatg ccctgacgcg cgagctcggg ccgccgtacg ttacgctgtc
     1321 tgcgtagata gttagattgc atacttcatg ttatttcgac gttgcccgtc gacctccccc
     1381 ttcatcgatc agccacagac gcgtcatcta cgcgtcaagg ttccacgaca tactggcgct
     1441 cgggcccttg cccgccgcgc agaggaggcg cagatgcagt cgtggaggag tgtcgcttcc
     1501 ggccgggtgc ccgctctggc gcgcggcgcc gaggacgtgc tcgccgtcga caacgagctc
     1561 gagcgcggcg cccccttctg cgccaacccc tcgtgcgcgc tgcatgtgcg gctcgccgac
     1621 tcacagg
//
LOCUS       DATLKL010000341         1943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_60329, whole genome shotgun sequence.
ACCESSION   DATLKL010000341 DATLKL010000000
VERSION     DATLKL010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60329"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VM713_04460"
     CDS             <1..992
                     /locus_tag="VM713_04460"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HVH83553.1"
                     /translation="ADQQPILARDLKAHGAMAAVLKTALAPNLVQTLENNPAFIHGGP
                     FANIAHGCNSVIATRSALKLADYVVTEAGFGADLGAEKFVDIKCRKAGLEPSAAVIVT
                     TVRALKYHGGVELADLKTENLKALDHGVVNLERHIDNVRHRYGLPCVVAINRRAEDTE
                     SELQLVIDRIAPHDVRVIPSTHFTDGGRGARDLAREVMRLCEQPNGFRYVYQDDWTLW
                     DKMKAIATQVYHASDITADTKVRAQIRQLQESGYGRYPVCVAKTQYSFSTDPKLRGAP
                     SHHVVNIREVRLAAGAEFVIMICGDIMTMPGLPRVPAANNIDVDDSGRITGLF"
     gene            1007..1909
                     /locus_tag="VM713_04465"
     CDS             1007..1909
                     /locus_tag="VM713_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83554.1"
                     /translation="MPFCEADPWRLQYFEGVVCPPEVRIPTEDPDGYQWNPAHRWVYN
                     KLAVAESQGLVCGPHGVVPPSFPVFSKPIINLHGMGVGSRVLPDRAHYERHETAGYFW
                     MPLLVGAHVSTDIAVQRGQARWMRHCLGVPGMGGTFDYWVIETGRRESLAGYLDAWVL
                     RHLAGYTGMFNAETIGDRIIEVHLRVADQWPDLYEPGWLESVVGLYADGNWSLASSER
                     RTGYSVALFGPHGRRYRHPPEQLVEEVRRMQRVSSVQITFHEDRAPGAHAMPPGGFRL
                     AVVNSTDLEAGLAARARLGAFWAV"
BASE COUNT          328 a          661 c          649 g          305 t
ORIGIN      
        1 gggccgacca gcagccgatc ctcgcgcggg atctcaaggc gcacggcgcc atggccgcgg
       61 tcctgaagac cgccctcgcg ccgaatctgg tgcagacgct cgagaacaac ccggccttca
      121 tccacggggg gccgtttgcc aacatcgcgc acggctgcaa ctcggtgatc gcgacccgca
      181 gcgcgctcaa gctcgctgac tacgtcgtga ccgaggccgg gttcggcgcg gacctcggcg
      241 cggagaaatt cgtcgacatc aaatgccgca aggcgggact cgagccctcg gcagcggtga
      301 tcgtcaccac ggtgcgggcg ctcaagtacc acggcggcgt cgagctcgcc gacctcaaga
      361 ctgagaatct caaggcgctc gaccacggtg tggtgaacct cgagcgccac atcgataacg
      421 tgcgtcaccg ctatggcctg ccgtgcgtgg tcgccatcaa ccgccgcgcc gaggacaccg
      481 agagcgagct gcagctggtg atcgatcgca tcgccccgca cgacgtgcgc gtcatcccct
      541 cgacgcattt caccgacggc gggcgcggcg cccgcgatct cgcccgcgag gtgatgcgcc
      601 tgtgcgagca gccgaacggt ttccgctacg tgtaccagga cgactggacg ctgtgggaca
      661 agatgaaggc aatcgccacc caggtctatc acgcgagcga catcaccgcc gataccaagg
      721 tgcgtgccca gatccgccag ctgcaggaaa gcggttacgg gcgctacccg gtgtgcgtcg
      781 ccaagacgca gtactccttc tccaccgacc cgaagctgcg cggtgcgccc tcgcaccacg
      841 tagtcaacat ccgtgaggtg cgcctcgccg ccggcgcgga attcgtcatc atgatctgcg
      901 gcgacatcat gaccatgccg ggactgccca gggtgccggc ggcgaacaac atcgacgtcg
      961 acgacagcgg ccggatcacc gggctgttct gaggccagga gggccagtgc ctttttgcga
     1021 ggctgatccc tggaggttgc agtacttcga gggcgtcgtc tgtccgccgg aagtccgcat
     1081 ccccaccgag gatccggacg gttatcagtg gaaccccgcg catcgctggg tctacaacaa
     1141 gctcgccgtg gccgagtcac agggcctcgt gtgcggtccg cacggcgtcg taccaccgtc
     1201 ctttccggtc ttcagcaagc cgatcatcaa tctgcacggg atgggagtcg gcagccgcgt
     1261 gctccccgat cgcgcgcact acgagcgcca cgagaccgcc gggtacttct ggatgccgct
     1321 gctcgtgggt gcccacgtga gcaccgacat cgccgtgcag cgcgggcagg cgcgatggat
     1381 gcggcactgc ctcggtgtgc cgggtatggg tggaaccttt gactactggg tgatcgagac
     1441 ggggcggcgc gagtcgctgg ccggatacct ggatgcgtgg gtgctgcggc acctggcggg
     1501 ctacaccggc atgttcaacg ccgagacgat cggcgatcgc atcatcgagg tccatctgcg
     1561 ggtcgccgac caatggccgg atctgtacga gcctggctgg ctcgagtcgg tggtcggact
     1621 ctatgcggac ggcaactggt cgctggcgag cagcgagcgc cgcaccggct acagcgtggc
     1681 gctcttcggt ccgcacgggc gcaggtaccg ccacccgcca gagcaactgg tcgaggaggt
     1741 acggcgcatg cagcgcgtga gcagcgtgca gatcaccttt catgaggacc gcgccccggg
     1801 agcgcacgcc atgccccccg ggggctttcg cctcgcggtc gtcaattcca ccgacctcga
     1861 agcgggcctg gcagcgcgtg cgcgcctcgg cgctttctgg gcggtctagg gacgcgccgc
     1921 ccgacgcacc tcacggattc cag
//
LOCUS       DATLKL010000342         2038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_161747, whole genome shotgun sequence.
ACCESSION   DATLKL010000342 DATLKL010000000
VERSION     DATLKL010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161747"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VM713_04470"
     CDS             complement(<1..419)
                     /locus_tag="VM713_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HVH83555.1"
                     /translation="MTELALGFIGLGNVGGQLAQNLLRHGVDLTVRDLDPGRVAPLVA
                     RGAKAAATARELAERVDLVITCLPSPVVSAAVMEAPDGVLAGLSAGKIWLEVSTTDAA
                     EVRRLAARVEALGATAMDCPVSGGCHRAASGNISIFAG"
     gene            479..1024
                     /locus_tag="VM713_04475"
     CDS             479..1024
                     /locus_tag="VM713_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HVH83556.1"
                     /translation="MSARLALTAVLLAVVAADTTSSGAQQPPATPAPYPLDNVPEKMP
                     FDIPYGAPISLERAQAAIAATAAEARKHDWKLCIAVVDSGGNLVAFQRMDGAQLASVQ
                     ISEHKARASATFRRETKVFETAIQQSGYNYVLTLDGVIGSRGGMPLVEGGKLIGAIGC
                     SGGTGSQDEVACKAGVATIAK"
     gene            1032..1409
                     /locus_tag="VM713_04480"
     CDS             1032..1409
                     /locus_tag="VM713_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVH83557.1"
                     /translation="MQQAVHKYLAALEAGDAERAAALFVPEGWVQSPFLGRLPVRAFV
                     TKVASASQRSKLTVHDVLVSAEGHLRAVAYYLYDWTLADGSHVAFDCTDVFNFDRETG
                     KIESIVLVYDTQPVRGVVEKKYP"
     gene            complement(1452..>2038)
                     /locus_tag="VM713_04485"
     CDS             complement(1452..>2038)
                     /locus_tag="VM713_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVH83558.1"
                     /translation="TKLAGGSAAGAESFARSLERLRTRRLDLLLVHNLNGVADLIPRM
                     QEWKQQGRVRYIGVTTSRGGQHAALADCMRTYPLDFIQVDYSIANREAAETILPLALE
                     RRMAVLVNLPFGRSSLFREAAGRPLPPWAADIDVKSWAQYFLKYVIGHPAVTCAIPGS
                     TQLSHLEDNQGAARGRLPDAGMRRRMEEYWDTRA"
BASE COUNT          331 a          692 c          716 g          299 t
ORIGIN      
        1 ccggcgaaga tcgagatgtt gccgctcgcc gcgcggtggc agccgccgga gaccggacaa
       61 tccatcgcgg tcgcacccag ggcctcgacg cgcgcggcga gccgccggac ctcggcggca
      121 tcggtggtgc tcacctcgag ccagatcttc cccgcggaca ggcccgcgag caccccgtcc
      181 ggcgcctcca tcaccgcggc ggagaccacc ggggagggca ggcaggtaat caccagatcg
      241 acgcgctcgg cgagctcgcg cgccgtggcg gccgccttgg cgccgcgggc caccaggggc
      301 gcgacgcgcc cggggtcgag gtcgcgcaca gtaagatcca cgccgtggcg cagcaggttc
      361 tgggccagct gtccgccgac attgccgaga ccgatgaaac cgagagcgag ttcagtcatc
      421 ggcgtaagat acctcaagcc tcatcgcctg gccggcaact aaaaaaagga gcagcaccat
      481 gtccgcgagg ctcgcactga ccgcagtact cctggccgtc gttgcggcgg acaccacctc
      541 gagcggcgcg cagcagccgc cggcgacgcc ggcgccgtat ccgctcgaca acgtgccgga
      601 gaagatgccg ttcgacattc cctacggggc gccgatctcg ctcgagcgcg cccaagccgc
      661 catcgcggcc accgccgccg aggcacgcaa gcacgactgg aagctgtgca tcgcggtggt
      721 cgactcgggc gggaacctgg tcgccttcca gcgcatggac ggagcgcagc tcgcctcggt
      781 acagatctcc gagcacaagg cgcgcgcttc ggccacgttc cgccgcgaga ccaaggtgtt
      841 cgagaccgca atccagcaga gcggctacaa ctatgtcctg acgctcgacg gcgttatcgg
      901 ctcgcgtggc ggcatgccgc tggtcgaggg cggtaagctc atcggtgcga tcggctgctc
      961 gggcggcacg gggtcgcagg acgaggtcgc ctgcaaggcg ggcgtcgcca ccatagcgaa
     1021 gtgagggatc tatgcagcaa gccgtgcaca agtacctggc ggcgctcgaa gcaggcgatg
     1081 ctgagcgagc ggcggccctg ttcgttccgg agggctgggt acagtcgccc tttctcggcc
     1141 ggcttccggt gcgcgccttc gtgacgaagg tggcgagcgc ctcgcagcgc tcgaagctca
     1201 ccgtgcacga cgtgctggtg agcgccgagg ggcacctgcg ggcggtggcg tactacctct
     1261 acgactggac gctcgctgac ggctcgcacg tcgccttcga ctgtaccgac gtattcaact
     1321 tcgatcgtga gaccggcaag atcgagtcga tcgtgctggt ctacgatacg cagccggtgc
     1381 gcggcgtggt cgagaaaaaa tatccctgag gcgacccagg gcggcggatg ctccgggcag
     1441 gcgcggctgc ttcaggctct ggtgtcccag tattcctcca tgcggcggcg catgcccgcg
     1501 tccgggagcc gcccgcgcgc cgcgccctga ttgtcctcga ggtggctgag ctgcgtggag
     1561 ccggggatcg cgcaggtcac cgcggggtgc ccgatgacgt acttgaggaa gtactgcgcc
     1621 cagctcttga cgtcgatatc ggccgcccat gggggcaggg gccggccggc ggcctcgcgg
     1681 aacagcgagg agcgcccgaa gggcaggttc accagcaccg ccatccggcg ctcgagcgcg
     1741 agcggcagga tggtctcggc tgcctcgcgg ttggcgatcg agtagtccac ctggatgaaa
     1801 tccagcgggt atgtgcgcat gcagtcggcg agcgccgcat gctgaccgcc gcgggaggtg
     1861 gtcacgccaa tgtagcggac tcgtccctgc tgcttccact cctgcatcct gggaatgaga
     1921 tccgccacgc cgttcaggtt gtgtaccagc agcaggtcga ggcgccgcgt ccgcagacgc
     1981 tcgagcgagc gcgcaaagct ctccgcaccc gccgcgctgc cgccggcgag cttggtcg
//
LOCUS       DATLKL010000343         3468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_150702, whole genome shotgun sequence.
ACCESSION   DATLKL010000343 DATLKL010000000
VERSION     DATLKL010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150702"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VM713_04490"
     CDS             complement(<1..338)
                     /locus_tag="VM713_04490"
                     /inference="COORDINATES: protein motif:HMM:NF026143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4478 family protein"
                     /protein_id="HVH83559.1"
                     /translation="MASELSEFVDARVGPRGSLENLSQTEIDKLLDAGQAGLYPLFRR
                     CALAVLNSGARTDNAKEIFDRYRDFDIRIVRHAWGVKLELKNAPAAAFVDGQMIRGIK
                     EHLFAVLRDVV"
     gene            complement(434..970)
                     /locus_tag="VM713_04495"
     CDS             complement(434..970)
                     /locus_tag="VM713_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HVH83560.1"
                     /translation="MIFRDLSAILVLGCAAVAAHGDSLPVTEPVALKSDTLPAVDRVV
                     VKKTERRMYLMHGGNVVRTYHVALGLSPVGQKERSGDFRTPEGSYHLDVRNPRSDYFL
                     SIKVSYPNQTDVKRARARHWDTGGSIMIHGLPNLMKHDPDYYLNHDWTDGCIAVSNAD
                     MVEIWMLVPDSVPIDIDP"
     gene            complement(1055..3046)
                     /locus_tag="VM713_04500"
     CDS             complement(1055..3046)
                     /locus_tag="VM713_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HVH83561.1"
                     /translation="MKPTPRQARAPAILLAWLWALCCAPPLAAEGAMARPAALEPDVQ
                     FWIRVYTQTDTNAGFLHDQYNLAVVYETLHFAPDASQSAREQEVDAHRDRITAALRRI
                     AAAGDAPLSEEDQRIKDLWGSASPGRLREAVDDIRFQLGQADRFRAGLIRAGAWETHI
                     AETLANKGLPAELAVLPHVESSFNPSAYSKVGAAGLWQFMRSTGRRYMRIDRSVDDRL
                     DPFRSTEAAAQLLGYNYRVLGTWPLAITAYNHGTAGMLRAKETLGTDDIVAIVRRYNS
                     RTFGFASRNFYVSFLAALDIDHNPDKYFGAVQKQSEARFQVVSVPGYVPMVTLERVLN
                     VRPEELRALNPALLRPVWDGQLRVPRDYRLRLPQEAEQWTTEQLAHQLNPRDLYAGQP
                     QPKRYKVQKGDTLARVAERYGVPIDTLARMNRLRVSAALRVGRTIELPEGATVQVASA
                     APAPAPAAAAKPDLGAVSAAVAQRESAEDAAAVATITVVASREQPVSAAQAEALSPAL
                     GPAADIQQSADPTDYSVRSDDSIRVAAEETLGHYADWLSVSAQRLRELNHMSRAKPVL
                     VGHRLKLDFHKVSHDVFEQHRREYHRELQAAYFAAHRISGTEIYIARPGDSLWTVTKR
                     ARLLPEWLLVQYNPDVDFSELRAGMQIVIPRVEEVENSG"
BASE COUNT          565 a         1135 c         1173 g          595 t
ORIGIN      
        1 accacgtcgc ggagcaccgc gaacaggtgt tccttgatgc cgcggatcat ctggccgtcg
       61 acgaaggcgg ccgccggagc gttcttcaat tccagcttca cgccccaggc gtggcgcacg
      121 atgcggatgt cgaagtcgcg atagcggtcg aagatctcct tggcgttgtc ggtgcgcgcc
      181 cccgaattga gcaccgcgag cgcgcagcgg cggaacagcg gatagaggcc cgcctgtccg
      241 gcatccagca gcttgtcgat ctccgtctgg gaaaggttct cgagactgcc gcgcggtccg
      301 acgcgggcgt cgacgaattc tgagagttcg ctggccacgg cttcagggac gttatgtcag
      361 gtcgcgattt ctttcgggac ggtgccgcga gcgtccccgc tcgctcgcga ggcacggtcg
      421 caccgcgcgc ttcttaagga tcgatgtcga tcgggaccga gtcggggacc agcatccaga
      481 tctccaccat gtccgcgttg gagacggcga tgcagccgtc ggtccagtcg tggttcaggt
      541 agtagtccgg atcgtgcttc atgaggttcg gaagcccgtg gatcatgatc gagccgccgg
      601 tatcccagtg ccgcgcgcgt gcgcgcttca cgtccgtctg gttcggatac gacaccttga
      661 tcgagaggaa gtaatcgctg cgcggattgc gcacgtcgag atgatagctg ccttcgggcg
      721 tgcggaagtc gcccgagcgc tctttctgtc cgaccggcga gagaccgagc gccacgtggt
      781 aggtgcgcac gacgttgccg ccgtgcatga ggtacatccg ccgctcggtc ttcttcacca
      841 cgacgcgatc gaccgcgggc agcgtatcgc tcttgagcgc taccggctcg gtcacgggca
      901 gcgagtcgcc gtgcgccgcc accgcggcgc agccgagcac gagaatcgca gataagtccc
      961 tgaaaatcat gactttccgt agtccgtaag ccaccttgca acgattatag ccagcgccgg
     1021 ggaccgcgct atgtacctgg ttcacagtag gccttcaccc gctgttttcg acctcttcga
     1081 cccgcggaat gacgatctgc atacccgccc gcagctcgct gaaatcgaca tccgggttgt
     1141 actgcacgag cagccattcc ggcagaagcc gggcgcgctt ggtgaccgtc cagagcgaat
     1201 ccccgggacg ggcgatgtag atctcggtgc ccgagatgcg gtgcgccgcg aagtaggccg
     1261 cctgcagctc ccggtgatat tcgcgccggt gctgctcgaa gacatcgtgc gacaccttat
     1321 ggaaatcgag cttcagtcga tgcccgacca gcaccggctt ggcccggctc atgtgattca
     1381 gctcgcgcag gcgctgcgcg ctcacgctga gccagtcggc gtagtgcccg agcgtctcct
     1441 cggcggcaac gcggatcgag tcatcgctcc gtacgctgta gtcagtcggg tcggcgcttt
     1501 gctgaatgtc ggcggcgggc ccgagcgccg gactcagcgc ttcggcctgg gcggccgaca
     1561 ccggctgctc gcggctcgcg accacggtga tggtggccac cgccgcggcg tcctcggcgc
     1621 tctcgcgctg cgcgaccgca gccgacaccg cgcccaggtc cggcttggca gcagccgctg
     1681 gcgccggcgc cggcgcggcg gaagccacct gcaccgtcgc gccttccggc agctcgatgg
     1741 tgcggccgac cctgagagcg gcgctcacgc gcagccggtt catgcgcgcg agcgtatcga
     1801 tcggcacgcc atagcgctcg gcgaccctcg cgagcgtatc tcccttctgc accttgtagc
     1861 gcttcggctg cggctgtccg gcgtagaggt cgcgcggatt gagctgatgc gcgagctgct
     1921 cggtcgtcca ctgctccgct tcctggggca accgcagccg gtaatcgcgc ggcacgcgca
     1981 gctgcccgtc ccacaccggg cgcagcagcg ccggattgag cgcccgcagc tcctcgggtc
     2041 gcacgttgag cacccgctcg agagtcacca tcggcacgta gcccgggacc gacaccacct
     2101 ggaacctcgc ctcgctctgc ttctgcacgg cgccgaagta cttgtcgggg ttgtgatcga
     2161 tgtcgagcgc ggcgaggaac gacacgtaga agttgcgcga ggcgaagccg aaggtgcggc
     2221 tgttgtagcg gcgcacgatg gcgacgatgt cgtcggtgcc gagcgtctcc ttggcgcgca
     2281 acatgccggc ggtgccgtgg ttgtaggcgg tgatcgcgag gggccaggtg ccgagcaccc
     2341 gatagttgta gccgaggagc tgggcggcgg cctcggtcga gcggaacgga tcgagccgat
     2401 cgtcgaccga gcggtcgatg cgcatgtagc gccggccggt cgaacgcatg aactgccaca
     2461 ggccggccgc gccgaccttg gaataggcgg aggggttgaa cgaggactcg acgtgcggca
     2521 gcaccgcgag ctcggcgggg agccccttgt tggcgagggt ctccgcgatg tgcgtctccc
     2581 aggcgccggc gcggatcagc ccggcgcgga agcggtccgc ctgcccgagc tggaaccgga
     2641 tgtcgtcgac cgcctcgcgc agcctccccg ggctggccga gccccacagg tccttgatgc
     2701 gctgatcctc ttccgacagt ggcgcatccc ctgcggcggc gatgcggcgc agcgccgcgg
     2761 tgatccgatc acggtgcgca tccacctctt gctcgcgcgc gctctgcgag gcatcgggtg
     2821 cgaaatgcaa cgtctcgtag acgaccgcga ggttgtactg gtcgtggagg aatcccgcgt
     2881 tggtatcggt ctgcgtgtac acgcggatcc agaactgcac gtcgggctcg agtgccgctg
     2941 gccgcgccat ggcgccctcg gcggccaggg gcggcgcgca gcacagggcc cagagccaag
     3001 ccagcaggat tgcgggagct cgcgcttgac ggggtgtcgg cttcatttac accccgatca
     3061 cagccatgtt atcaagtgaa ttcaatggtt tgctgttcat ccggcctccc tgccgagaaa
     3121 cgcgggctgc ggaagcgtcg agtatcttga cacttgtgcc gtccagaggc ggggcagtgg
     3181 accgcagcca ccgcatctta ggctcaagcg gcgccccggc gggtagggtt agacggtaga
     3241 agacggcccc ggatcgtgcg ccgcgtcaca tttgtacagc ccctgcgcgg cagcatgtcg
     3301 cgccgattca gagatccatc ctcctggcac gggtgttgct catatccacc catctgcggc
     3361 acagccgcat tttgggaagc aagcgagcca gggcaaccga cgagaatttc cgggtcagga
     3421 acaaaagcaa ttcgccagac accggaagca tcagataaca acgagaag
//
LOCUS       DATLKL010000344         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_184491, whole genome shotgun sequence.
ACCESSION   DATLKL010000344 DATLKL010000000
VERSION     DATLKL010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184491"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1181)
                     /gene="plsX"
                     /locus_tag="VM713_04505"
     CDS             complement(132..1181)
                     /gene="plsX"
                     /locus_tag="VM713_04505"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HVH83562.1"
                     /translation="MTEGLTIALDAMGGDNAPAVVIKGAAITRVRFPNVRYLLFGDER
                     KMRLLLKRKPKLRKLVTLVHTDKVVLGDAKPSIALRQGKGSSMWEAIDSVRQGQAAAV
                     VSAGNTGALMGMAKLILGTLPGIDRPAIAAFFPTMRGESVMLDLGANVECDANNLVQF
                     AVMGEVFARTVLGLQQPTIGLLNVGEEPVKGSEAVRTASTILRDTRLPIKFEGFVEGD
                     DIAAGTVDVIVTDGFTGNIALKTAEGTARLLREFVRQAFRSSTLAKLGFIMARRALRK
                     MQVRTDPRRYNGAMFLGLNGIAVKSRGSADALGFANAIGVAIDLAAGGFNDRIKEEFH
                     RLNAGQDDKPHAAAE"
     gene            complement(1217..1399)
                     /gene="rpmF"
                     /locus_tag="VM713_04510"
     CDS             complement(1217..1399)
                     /gene="rpmF"
                     /locus_tag="VM713_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HVH83563.1"
                     /translation="MAVPKKKISKSRRDMRRSHHALTKATYVECPNCGELMRPHHICR
                     ACGHYRGREVVEAKQA"
     gene            complement(1484..>2057)
                     /locus_tag="VM713_04515"
     CDS             complement(1484..>2057)
                     /locus_tag="VM713_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HVH83564.1"
                     /translation="ILMNKHPPIAIEFSRIVAVDRLDQGEVVEEISAGAQERLALAAR
                     FDLVALDSLSAQVSLRRIGRGPVVRVEGRLSADVVQNCVVSLEPVHNRVEEDFVLLYA
                     PEGSGGGEGHVLEEGFDLADDEWPEPLEEGRIDIGEAVAQQLALALDPYPRKPGVRLE
                     DVIGRHEGIGTDEPPASPFAALARLSRRDS"
BASE COUNT          346 a          708 c          651 g          352 t
ORIGIN      
        1 cgctcgcgaa ttcccgtgcg ctcgacgatc cattcgtcgg aggtgtcgag gcgcctcgcc
       61 agttcatcat tggtgacgac gcgcgcgggc agataagccc cgcagccgac gaccctggaa
      121 tggcgctgca tctactcggc cgcggcgtgg ggcttgtcgt cctgaccggc gttcaagcgg
      181 tggaattcct ctttgattcg gtcattgaag cccccggcgg ccaggtcgat cgcgactccg
      241 atggcgttgg caaagccgag ggcgtccgcg ctgccacggc tcttcaccgc gatgccgttg
      301 agaccgagga acatcgcgcc gttgtagcgg cggggatcgg tgcgcacctg catcttcctg
      361 agtgcgcgcc gcgccatgat gaagccgagc ttggcgagcg tcgagctcct gaaggcctgg
      421 cgtacgaatt cgcgcagcag ccgcgccgtg ccctctgcgg tctttagcgc gatgttgccg
      481 gtgaatccat cggttacgat cacgtccacc gtccccgccg cgatgtcgtc gccctcgacg
      541 aacccctcga atttgatcgg cagccgggta tcgcgcaaga tcgtgctcgc ggtgcgcacg
      601 gcctcgctcc ccttgaccgg ctcctcgccc acattgagga gcccgatcgt gggctgctgg
      661 aggccgagga ccgtgcgggc gaagacctcg cccatcaccg cgaactgcac gagattgttg
      721 gcgtcgcact cgacgttggc gcccaggtcg agcatcacac tctccccgcg catggtgggg
      781 aagaaggcgg cgatcgccgg ccggtcgatc ccaggaagcg tgcccagaat cagcttggcc
      841 atgcccatga gcgcgcccgt attgcccgcc gagaccacag cggccgcctg accctgacgg
      901 acggaatcga tggcctccca catgctcgat cccttgccct ggcgaagggc gatcgacggc
      961 ttggcatcgc caaggacgac cttgtccgtg tggacgagcg tgaccagctt gcgcagcttc
     1021 ggcttgcgct tgagcagcaa tcgcattttg cgctcgtcgc caaacaggag atagcggacg
     1081 ttcggaaagc ggacgcgggt gatcgcagcg cccttgatca ccacggcggg cgcgttatct
     1141 ccgcccatcg cgtcgagcgc gatcgtcaac ccctcggtca cgcgcatcga acctccccgc
     1201 gggcgccaca agcggctcac gcctgcttag cctccaccac ctcgcggccg cgataatggc
     1261 cgcacgcccg gcaaatgtgg tgcggccgca tcaactcgcc gcagttcggg cactcgacgt
     1321 aggtcgcctt cgtgagcgcg tgatgggagc ggcgcatgtc gcggcgcgac ttcgagatct
     1381 tcttcttcgg gacggccatg acggatttct ctcaaagaac cagattgggg aagcccgcct
     1441 aactatcgga aaaccgaagt gacggcaaga cgctaccgcc aatttatgaa tcccttcttg
     1501 acaaccgggc cagtgcggcg aagggactgg ccggaggctc gtccgtgcct atgccctcgt
     1561 gtctgcctat cacgtcctca agccgtaccc cgggcttgcg cgggtagggg tcgagggcca
     1621 gggcgagctg ctgggcgacc gcctcgccga tgtcaatccg cccttcctcg agcggctcgg
     1681 gccattcgtc gtccgcaagg tcgaagcctt cttccagcac gtggccctcg cccccgcccg
     1741 acccctccgg cgcgtagagg agcacgaaat cctcctcgac ccggttgtgc accggctcga
     1801 gcgagacgac acaattctgc accacgtcgg cggagaggcg accctcgacg cgcaccaccg
     1861 gcccgcggcc aatgcggcgc agagacacct gcgccgaaag gctgtcgagg gcgacgaggt
     1921 cgaagcgcgc cgcgagcgct aggcgctcct gcgcgccggc gctgatctcc tcaacgacct
     1981 cgccttgatc cagccggtcc accgcgacaa tgcgcgaaaa ctcgatcgca atcggggggt
     2041 gcttattcat gaggata
//
LOCUS       DATLKL010000345         1621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_37859, whole genome shotgun sequence.
ACCESSION   DATLKL010000345 DATLKL010000000
VERSION     DATLKL010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37859"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="VM713_04520"
     CDS             complement(<1..1446)
                     /locus_tag="VM713_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HVH83565.1"
                     /translation="MREDGRTRQRIIANLGRKEAVVARGDLDRLARSVARLAQRSMVL
                     AAVEGEAPPHAVCRRIGPALLFERLWQEIGCRAVLDELAAQRQFAFAAERAVFLTVLH
                     RLFVSGSDRAAEKWRADYRIDGTEELQLHHLYRAMAWLGEELPQAQQQGSAKLVPRCT
                     KDVIEEQLFARHRDLFAELSVVFMDTTSLSFEGQGGEALGRHGYSKDCRRDLDQMIVG
                     LVMDQDGRPLCCELWPGNTADVTTLLPVVDRLRARFGVGHLCVVADRGMISAQTIAAL
                     EKRKLEYILGVRERRSAEVRRTVIDDSTPFVPLVVPRAAGVLTELEAKQVKIGERRYI
                     VCRNLAEARRDAEQRAAILDGLRAKLAQGDKALVGNSGFRRYLKTISARHFAIDEERV
                     TEDARFDGLYVLRTNTKLTPLQVMLRYRDLLRVEQLFRQAKAVLATRPIYHSSDMAIR
                     GHVFCSFLALLLVKELEDRLRRNGVATEWGDI"
BASE COUNT          278 a          529 c          538 g          276 t
ORIGIN      
        1 gatgtcgccc cattcggtgg ccaccccgtt ccggcgcagc cgatcttcga gttctttgac
       61 gagcagcagc gccagaaagg agcagaacac atggccgcgg atcgccatgt cgctgctgtg
      121 gtagatcgga cgggtggcga gcacggcctt ggcttggcgg aacaactgct cgacccgcag
      181 caggtcgcgg tagcgcagca tgacctgcag cggggtgagc ttggtgttgg tgcgcagcac
      241 gtagagcccg tcgaagcgcg cgtcttcggt gacccgttcc tcgtcgatgg cgaagtgcct
      301 ggccgagatg gttttgagat aacggcggaa gccgctgttg ccaaccagcg ccttgtcgcc
      361 ttgggcgagc ttggcgcgca gcccgtcgag gatggcagcg cgctgctcgg cgtcgcgccg
      421 ggcctcggcg agattgcgac agacgatgta gcgccgctcg ccgatcttga cctgcttggc
      481 ctcgagttcg gtgaggacgc ccgcagcccg cggcacgacc agcgggacaa agggcgtgga
      541 atcgtcgatc acggtgcggc gcacctcggc gctgcggcgc tcgcgcacgc cgaggatgta
      601 ttcgagcttg cgtttctcga gcgcggcaat ggtctgggcg ctgatcatgc cgcggtcggc
      661 gaccacgcag aggtggccga cgccaaaacg cgcgcgcaat cggtcgacga ccggcaacag
      721 ggtggtcaca tcggcggtat tgccgggcca cagctcgcag cacagcggcc taccatcctg
      781 atccatgacc agcccgacga tcatctggtc gagatcgcgc cggcagtctt ttgagtagcc
      841 gtgacggcca agcgcttcgc cgccctgtcc ctcaaaggac aggctggtgg tatccatgaa
      901 caccacgctg agctcggcaa acaggtcgcg gtggcgtgcg aacagctgct cctcgatcac
      961 gtccttggtg cagcgcggca cgagcttggc ggacccctgc tgctgcgcct gcggcagttc
     1021 ttcgccgagc caggccatgg cgcggtagag atgatgcagc tggagctcct cggtgccgtc
     1081 gatgcggtag tcggcccgcc acttttcggc agcgcggtcc gaacccgaca cgaacagccg
     1141 gtggagcacc gtcaggaaca cggcgcgctc ggcggcgaac gcgaactgcc gctgcgcggc
     1201 gagctcatcc aataccgcgc ggcagccaat ctcctgccac agccgctcga acagcagcgc
     1261 cggaccgatc cgccggcaca ccgcatgggg tggggcctcg ccctcgaccg cggccaaaac
     1321 catcgaccgc tgcgccagac gggcgaccga gcgcgccaga cggtcgagat cgccgcgcgc
     1381 gacgaccgcc tccttgcgcc cgagattggc gatgatgcgc tgcctggtgc ggccgtcctc
     1441 gcgcacgttc tcgaccaggt agacataggt gtaggggcca atgcgcttgt cacgggcgaa
     1501 cataccttta gcatccgtag atcgcgcaca gagtcaatca gttattggcc tgctattggc
     1561 gattttcaga agaatttagc acctcagttt cggcctggct ttatcaaccc ccagtgtttc
     1621 t
//
LOCUS       DATLKL010000346         3300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_184512, whole genome shotgun sequence.
ACCESSION   DATLKL010000346 DATLKL010000000
VERSION     DATLKL010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184512"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2396)
                     /gene="gyrA"
                     /locus_tag="VM713_04525"
     CDS             complement(<1..2396)
                     /gene="gyrA"
                     /locus_tag="VM713_04525"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HVH83566.1"
                     /translation="MAEIAREVLPVSLEEEMRQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLHAMRELGNDFNKPYKKSARVVGDVIGKYHPHGDTAVYDTIVRLAQPFSMRYLL
                     VDGQGNFGSVDGDMPAAMRYTEVRMSRIAHELLADIDKETVDFVPNYDESESEPSVLP
                     TRIPNLLVNGQTGIAVGMATNIPPHNLTETINACLALLDEPALPLAAIMQHVPGPDFP
                     TGGIINGAQEIATAYRTGRGRLSVRARVSIEEVGRGDRQAIVVTELPYQVNKARLIER
                     IAELVREKQLDGIAADGLRDESDKDGMRVVIELKRGEVTEIVLNNLYAQTPMETVFGI
                     NMVALQDGQPKLMSLKEMLEALIRHRREVVTRRTIYDLGKARERAHILEGLAVALANI
                     DEVIALIKAAPAPPEARAALLARAWPPGLVTELLERAGAISTRPAGLAADFGLNGGGY
                     RLSEAQAQAILDMRLHRLTGLERNKIVEEYRGLLEQLRDLSDILARPERLTQVVRAEL
                     VEMRETYGDARRTEINRDHLDLTTEDLIEPQDVVVTLSHAGYAKAQPLTEYQTQRRGG
                     RGKTATAVKDEDFVERLFVAHTHDTVLCFSNRGKVYWLKVYELPQAGRGSRGKPMVNL
                     LPLGEGEKINTLLPVKQYDEQHFVFMATRAGTVKKTPLAAFSRPRSTGIIAVDLADND
                     QLVGVAITDGEREIMLVSSGGKALRFHESEVRHMGREAAGVRGIRLGTGQDLIALIVL
                     GVGEVLTASAAGYGKRTPLADFPAHGRGGQGVIALQTGGRNGDTVAALQVLPGQEIML
                     "
     gene            2611..>3300
                     /gene="ubiG"
                     /locus_tag="VM713_04530"
     CDS             2611..>3300
                     /gene="ubiG"
                     /locus_tag="VM713_04530"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HVH83567.1"
                     /translation="MSTPAATRTTNADPAELSKFEAVAARFWDSHGEFRPLHLLNPVR
                     AQYIATRAPLAGKHVLDVGCGGGLLAEALAALGAHVTGIDLAPGMIEVARLHAAESRL
                     AIDYRVADAGELAAAASGAFDVVTCMEMLEHVPEPAAMVTTLAALTRPGGALFVSTLN
                     RNLRSFLQAIVAGEYLLRLLPRGTHEYERLIRPAELARWGRAAGLTLLELRGIELNPF
                     TAQAALSGSVAV"
BASE COUNT          473 a         1152 c         1126 g          549 t
ORIGIN      
        1 atcagcatga tttcctggcc cggcagcact tgcagggccg ccacggtatc gccgttgcgg
       61 ccgccggtct gcagcgcgat cacgccctgg ccgccgcggc cgtgggccgg gaagtcggcg
      121 agcggcgtgc gtttgccgta gccagcggcg gaggccgtca gcacctcgcc cacccccagc
      181 acgatgagcg cgatcaggtc ctgcccggtc ccgaggcgga tgccgcgcac gccggcggcc
      241 tcgcgtccca tgtggcgcac ttcgctttcg tggaagcgga gcgccttgcc gccgctcgac
      301 accagcatga tctcgcgctc gccgtcggtg atcgcaaccc cgaccagctg gtcgttgtcg
      361 gcgagatcca ccgcgatgat gccggtcgag cgcgggcgcg agaacgccgc gagcggcgtc
      421 ttcttcaccg tgccggcgcg cgtcgccatg aacacgaagt gctgctcgtc atactgcttc
      481 accggcagca gcgtgttgat cttctcgccc tcgccgagcg gcagcaggtt caccatgggc
      541 ttgccgcgcg acccgcgccc ggcctggggc agctcataga ccttgagcca atagaccttg
      601 ccgcggttgg agaagcacag caccgtgtcg tgggtgtggg cgacgaacag cctctcgacg
      661 aaatcctcgt ccttgaccgc ggtcgcagtc ttgccgcggc cgccgcgccg ctgcgtctga
      721 tactcggtga gcggctgcgc cttggcatag ccggcgtgcg atagggtcac caccacgtcc
      781 tgcggctcga tcaggtcctc ggtggtgaga tcgaggtggt cgcggttgat ttcggtgcgg
      841 cgcgcgtcgc cgtaagtctc gcgcatctcg acgagctcag cgcgcaccac ctgggtgagg
      901 cgctcggggc gcgccaggat atccgacagg tcgcgcagct gctcgaggag accgcggtat
      961 tcctcgacga tcttgttacg ctcgaggccg gtgagccgat gcaggcgcat atcgaggatc
     1021 gcctgcgcct gcgcctccga gaggcggtag cccccgccgt tcaagccgaa gtcggcggcg
     1081 agtcccgcgg ggcgcgtcga gatggcgccg gcgcgctcga ggagctcggt caccagaccc
     1141 ggcggccagg cacgcgcgag cagcgccgcg cgcgcctcgg gcggcgcggg cgccgccttg
     1201 atgagcgcga tcacctcgtc gatgttggcg agcgcgaccg ccagtccctc gaggatgtgg
     1261 gcgcgctcgc gcgccttgcc gaggtcgtag atggtgcggc gggtgacgac ctcgcggcgg
     1321 tgccggatga gggcctccag catctccttg agcgacatga gcttcggctg cccgtcctgc
     1381 agcgccacca tgttgatgcc gaacaccgtc tccatcggtg tctgcgcgta gaggttgttg
     1441 aggacgatct cggtcacctc gccgcgcttc aactcgatga cgacgcgcat gccgtccttg
     1501 tcggactcat cgcgcagccc gtcggcggcg atgccgtcga gctgcttctc gcgcaccagc
     1561 tcggcgatgc gctcgatcag gcgcgccttg ttcacctgat agggaagctc ggtgaccacg
     1621 atcgcctggc gatcgccgcg accgacttcc tcgatgctca cccgggcgcg caccgacagc
     1681 cgcccgcgcc cggtgcggta ggcggtggcg atctcctgcg cgccgttgat gatgccgccg
     1741 gtgggaaagt cgggacccgg gacgtgctgc atgatggcgg cgagcggcag ggccggctca
     1801 tcgagcagcg cgaggcacgc gttgatggtc tcggtgaggt tgtgcggcgg gatgttggtc
     1861 gccatgccga ccgcgatgcc ggtctggccg ttcaccagca ggttcggaat gcgcgtgggc
     1921 agcaccgagg gctccgattc gctctcgtcg tagttcggga cgaagtcgac ggtctccttg
     1981 tcgatgtcgg cgagcagctc gtgcgcgatg cgcgacatgc gcacctcggt gtagcgcatg
     2041 gcggccggca tgtcgccgtc gaccgagccg aaattaccct ggccgtcgac cagcaggtag
     2101 cgcatggaga aaggctgcgc gaggcgcacg atcgtgtcgt acaccgccgt gtcgccgtgc
     2161 gggtggtact taccgatcac atcacccacc acacgcgccg acttcttgta gggcttgttg
     2221 aagtcgttgc cgagctcgcg catggcgtgc agcacgcgcc ggtgcacggg cttcaggcca
     2281 tcgcgcacgt cgggcaacgc ccgcccgacg atcacgctca tcgcatagtc gaggtaggac
     2341 tgccgcatct cctcttcgag actgacgggc agaacttcgc gggcgatttc agccattcac
     2401 tatcgggtag ggaggcccgt tggggccgtg aaataaagga gaaattctag cacgaagcaa
     2461 cgcgcggccc gcgcttaccc gaggtgctcg aggcgcgtcc cgcggccctt gtcaagccac
     2521 cggcgaaagc acgcggacgc gcccgttttg cgtgcgggcg ccgtatgcgg cgcgtcgata
     2581 tacttccgcc acatcgcacg acagctcacc atgtcaaccc ccgcagccac ccgcaccacc
     2641 aacgcagatc ccgccgagct cagcaagttt gaggccgtcg cggcccgctt ctgggacagc
     2701 cacggggaat tccgtccgct gcatctgctg aacccggtgc gcgcgcagta catcgccacg
     2761 cgcgccccgc tcgccgggaa gcacgtcctg gacgtcggct gcggcggggg actcctcgcc
     2821 gaggcgctcg cggcgctcgg cgcccatgtc accggcatcg atctcgcgcc cgggatgatc
     2881 gaggtcgcgc ggctgcatgc cgcggaatcc cgactcgcca tcgattatcg tgtcgccgac
     2941 gccggcgagc tggccgccgc ggcgtcgggc gcgttcgatg tcgtcacctg catggagatg
     3001 ctcgagcatg tgcccgagcc cgccgccatg gtgaccaccc tcgccgccct cacccgaccc
     3061 ggcggcgccc tgttcgtttc gacgctcaac cgcaatctgc gctcgttcct ccaggcaatt
     3121 gtcgccgggg agtatctgct gcgcctcctg ccgcgcggca ctcacgagta cgagcggctg
     3181 atccgtcccg cggagctggc ccgctggggg cgcgccgccg ggctgacgct cctcgagctg
     3241 cggggcatcg agctcaatcc cttcaccgcc caggcggcgc tctccggcag cgtggcggtc
//
LOCUS       DATLKL010000347         3356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_82893, whole genome shotgun sequence.
ACCESSION   DATLKL010000347 DATLKL010000000
VERSION     DATLKL010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82893"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..3223)
                     /locus_tag="VM713_04535"
     CDS             complement(212..3223)
                     /locus_tag="VM713_04535"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HVH83568.1"
                     /translation="MAFSRRQVIVLAAAALLLGAYALAGFVAVPHFARQGAGDFVRTH
                     YARALTIGEIRFNPFTLNLDVTGVALPDADGQTLLSFDRLHVGLQVASLWRLGPSFSE
                     IVLERPYVRAVIRPDGALNLADLGKGFPPAPPEQPQKKPAPMRLYIGRLAVIDGGTTF
                     EDRTRATPFRADFKPIAFELRDFSTTAATGDAYTLTAASPQGERLDWSGTVRLAPLGS
                     QGKFAITDLKARTLWSYLSASLPFEIDSGVIALNGDYDVGSAGGPITLKLNVHNTTVS
                     ALGVKPRGADADYISLRRIEVDDARIDLGGRAVEVAKVQLAGGDIRAWLSEQGRINLL
                     DLLAPAPAAGAGAPAGGAPAAAPAAAPAAAGGSPWSLSVPDISVAGLKVSAEDRQVKP
                     AAVVVLEPLEVHIAGFSLAPDDLLDIRLDSTVNTTGKVSATAKVAPRSGAVTAHVEAE
                     HLGLPALQPLLAQYTSMTLLKGELGARVDLERGADGTLGVKGNMRIADLSTVDNELQK
                     DFIRWKDLRIVDVSYRSQPASLRIGSVTALEPYVRMIIAPDRTVNITQVLTPPGGKAA
                     PAVPGAAAAPAPRKLKQAATAPAAASTAPLTPFPVSIGAFRMVNGSADYTDLWIKPSF
                     VLGMQALNGAVTGMSSDPRSRARVQLDGKLASFSPLRIAGEVNLLSAALYTDVTLSFK
                     DLDLTIVNPYSGYFVGYKIDKGKLSVDVSYKIEQRQLNAAQHFIVDQLELGDRVESPE
                     AMHLPLKLAVTLLKDRNGVIDLPLPMSGSLDDPTFRIGPIIWKMFVNLITKAATAPFA
                     LLGHLFGGGENMNVVEFAAGSGELSPAAQEQLAALNRAMKERPQLQLDVPLVASMGID
                     RPQLAQVQLRAALTARVASSRQGRKHPEEAAELALADPSRHLQLLIEQYQADLGRDAA
                     LPASAQAVQAGNKKEPADLDAAVADLETALINHIQVPDAELAGLGRARAQAIQKALLA
                     DGQINPARVFIVNALPKPDSGDTVKVELAVH"
BASE COUNT          549 a         1099 c         1147 g          561 t
ORIGIN      
        1 acctgccgcc gccccgacaa gtcccccgtc agccagtcag gtcaagcagc ctgaagaact
       61 tcaatggaac gcggtcgggg ctaccgctca atgcgtagac ggaacttttt accacgggac
      121 attcaaccag cacgcctgtg ccgatcacca tggcgttcga aggttgttac acacgcgcga
      181 tgagctcctg atccggtagt gtgcccgcgg ctcagtgaac cgccagctcg actttcacgg
      241 tgtcaccgct atccggcttc ggtaacgcgt tgacgatgaa cacgcgcgcc gggttgatct
      301 ggccgtctgc aaggagcgct ttctgaatcg cctgggcgcg cgcgcggccg agcccggcga
      361 gctcggcgtc cgggacctgt atgtggttga tgagcgcggt ctcgagatcg gcgacggccg
      421 catcgagatc ggccggctcc ttcttattgc ccgcctgcac cgcctgggcc gaggcgggca
      481 gcgctgcatc cctgccgaga tccgcctgat actgctcgat gaggagctgc aaatgcctgc
      541 tgggatcggc gagcgcaagc tccgcggctt cctccgggtg cttcctgcct tggcggctac
      601 tcgccacgcg agccgtgagc gccgcgcgca actgcacttg cgccagctgc ggccgatcga
      661 tccccatcga ggcaacgagc ggcacatcga gctggagctg cggccgctcc ttcatggccc
      721 gattcagcgc cgcgagctgc tcctgcgccg ccggactgag ctcgccgctg ccggcggcga
      781 actccacgac gttcatgttc tcaccacccc cgaagaggtg tccgaggagc gcgaacggcg
      841 cggttgcggc cttggtgatc agattgacga acatcttcca gatgatcggc ccgatcctga
      901 aggtcggatc atcgagcgag cccgacatcg gcagcggcag gtcgatcacg ccgttgcggt
      961 ccttgagcag cgtcaccgcc agcttcagcg gcagatgcat cgcttcgggg ctctcgaccc
     1021 ggtccccgag ctcgagctga tcgacgatga agtgctgcgc ggcgttcagc tgccgctgct
     1081 cgatcttgta ggagacatcg accgatagct tgcccttgtc gatcttgtag ccgacgaagt
     1141 acccggagta cggattgacg atggtcagat cgagatcctt gaagctcagc gtgacgtcgg
     1201 tgtagagcgc cgccgacaga aggttcacct cgccggcgat acgtagcggc gagaagcttg
     1261 cgagcttgcc gtcgagctgc acgcgggcgc gcgagcgcgg atcggaggac atcccggtca
     1321 cggcgccatt gagcgcctgc ataccgagca cgaagctcgg cttgatccac aggtcggtgt
     1381 agtccgccga gccgttgacc atgcggaaag ccccgatcga gaccgggaat ggtgtaagcg
     1441 gggccgtcga ggctgcggcg ggtgcggtgg ccgcctgctt aagcttgcgc ggggcgggcg
     1501 cagcggctgc gcctggcacg gcaggcgcag cttttccccc cggcggtgtc agcacctgtg
     1561 tgatgttcac ggtgcggtcg ggcgcgatga tcatacgcac gtaaggctcg agcgccgtca
     1621 cgctcccgat acgcaggctc gccggctgcg agcggtagct gacatcgacg atgcgcaggt
     1681 ccttccaccg gatgaagtcc ttctgcagct cgttgtccac ggtgctcaga tccgcgatgc
     1741 gcatattgcc cttgacaccg agggtgccat cggcgccgcg ctcgaggtcg acacgggcgc
     1801 cgagctctcc cttgagaagg gtcatcgagg tgtattgcgc gagcagaggc tgcagagccg
     1861 gcagcccgag atgctcggcc tcgacgtgcg ctgtgaccgc cccggaccgc ggcgccacct
     1921 tcgccgtcgc tgacaccttg ccggtggtgt tgacggtcga gtcgagccgg atgtcgagca
     1981 ggtcatcggg cgcgaggctg aatcccgcga tgtgcacctc gagcggctcc agcaccacga
     2041 ccgccgcggg cttcacctgc cgatcctcgg cggatacctt gaggccagcg accgatatat
     2101 caggtacgga cagcgaccag ggggaaccgc ctgccgccgc gggcgccgcc gcgggcgcgg
     2161 ccgcaggcgc gcctccggcg ggagcgcctg ctccggccgc cggcgcgggg gcgagcagat
     2221 cgaggagatt gatgcgtccc tgctcactga gccacgcccg gatatcgcca ccggcaagct
     2281 gcaccttggc tacctcgacg gcgcgtccgc caagatcgat gcgcgcgtcg tcaacctcga
     2341 tgcgccggag cgagatgtag tcggcatccg cgccgcgagg cttcacgccg agagcgctga
     2401 cagtcgtgtt gtgcacgttg agtttcaggg tgatcgggcc gccggcgctt ccgacgtcgt
     2461 agtcgccgtt gagcgcgatg acgcccgagt cgatctcgaa cggcaggctc gcggacagat
     2521 agctccacag ggtgcgcgcc ttgagatcgg tgatcgcgaa tttgccttgg gagccgagcg
     2581 gcgcgaggcg caccgtcccg ctccagtcga gccgctcgcc ctgtggggaa gcggccgtga
     2641 gcgtgtaggc atcgccggtc gccgccgtcg tgctgaagtc gcgcagctcg aaggcgatcg
     2701 gcttgaagtc ggcgcggaat ggagtcgcgc gcgtgcgatc ctcaaacgtc gtgccgccgt
     2761 cgatcacagc caggcgaccg atataaagcc gcatgggcgc gggttttttc tggggctgct
     2821 cgggcggcgc gggcggaaag cccttgccaa gatctgccag gttgagcgcg ccgtccgggc
     2881 gtatcacggc ccgcacgtag ggccgctcga gcacgatctc gctgaagctc gggccgagtc
     2941 gccagagact cgccacctgc agcccgacgt gcaggcggtc gaatgagagc agcgtctgac
     3001 cgtccgcgtc cgggagcgcg accccggtga cgtcgagatt cagcgtgaag ggattgaagc
     3061 ggatctcgcc gatcgtcagg gcgcgggcgt agtgggtgcg cacgaagtcg cccgcgccct
     3121 ggcgtgcgaa gtgcggcacc gcgacaaacc ccgcaagcgc gtacgccccg agcagcaacg
     3181 cggccgcggc gagcacgatg acctgacgcc gcgagaaggc catgagccgc gaatctcctg
     3241 gttttttcta ggcgctcagc tttgtggggc tatcgtagat cagcgcagcg cccgggcacc
     3301 atcggcgcga ttacatacag agggctgcac gactttgagg cagtctccga ccgctt
//
LOCUS       DATLKL010000348         1508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_195670, whole genome shotgun sequence.
ACCESSION   DATLKL010000348 DATLKL010000000
VERSION     DATLKL010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195670"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VM713_04540"
     CDS             <1..1049
                     /locus_tag="VM713_04540"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HVH83569.1"
                     /translation="HLGDAALCLGAPSAELRVQVRVGTALIDEAWADLKRAWSETSWR
                     MRRLRDDPQCADEEFAAQSAEADPGLSVDLQFDPEEDIAAPYVARGVRPKVAILREQG
                     VNSQTETAAVFAQAGFEPHDVHMTDLLTGVRTLDGFKGLVACGGFSYGDVLGAGEGWA
                     KSILYTDAVRSEFERYFARSDSFALGICNGCQMFAALKSLIPGTAHWPRFVQNRSEQY
                     EARFPLVELLHSPSVLLEGMAGSFLPIAVSHGEGRAEFPSPQAAAACAASALVGFRYV
                     QHDRSVATTYPANPSGSPFGIAALCNADGRVTITMPHPERSFRYLQNSWRPRAAGSYS
                     GWMRLFRNARRFVG"
     gene            complement(1050..>1508)
                     /locus_tag="VM713_04545"
     CDS             complement(1050..>1508)
                     /locus_tag="VM713_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83570.1"
                     /translation="TEVERAREKFSFAYIPEGVELLFREALACFSQGAFNAFASMCRR
                     TAQAMFADLGEAGKLRLFDELNGLRDLTDIAPDLFTRMRNVLFGAELDARAGIPLLDG
                     YQAGIMLEVMKDLLYEAYVRKGRLQQAIMVRRFFLDETGSHKVTPLQSAS"
BASE COUNT          235 a          505 c          532 g          236 t
ORIGIN      
        1 atcacctcgg cgatgcggcg ctctgcctcg gcgcaccgag cgcggagctg cgcgtgcagg
       61 tgcgggtcgg cacggcgctg atcgatgagg catgggcgga cctcaagcgc gcctggtccg
      121 agacctcgtg gcgcatgcgg cggctgcgcg atgacccgca gtgcgccgac gaggagttcg
      181 ccgcgcagag cgcggaagcg gaccccgggc tcagcgtcga tctccagttt gaccccgagg
      241 aggacatcgc ggcgccctac gtcgcgcgcg gcgtgagacc taaggtcgcg atcctgcgcg
      301 agcagggcgt caacagccag accgagacgg cggcggtgtt cgcccaggcg ggcttcgagc
      361 cgcacgatgt gcacatgacg gacctgctca ccggcgtgcg caccctcgat gggttcaagg
      421 ggctcgttgc ctgcgggggg ttcagttacg gcgatgtgct cggggcgggc gagggctggg
      481 cgaaatcgat cctctacacg gacgcggtga ggagcgagtt cgagcgctac ttcgcgcgca
      541 gcgacagctt tgcgctcggc atctgcaacg gctgccagat gtttgccgcg ctgaagagcc
      601 tcatcccggg taccgcgcac tggccgcgct tcgtgcagaa ccgcagcgag cagtacgagg
      661 cgcgcttccc gctggtcgag ctcctgcact caccctcggt gctgctcgag ggaatggccg
      721 gatcgttcct gccgatcgcg gtgtcgcacg gggaggggcg ggccgaattt ccctcgccgc
      781 aagcggccgc ggcctgtgcg gcgagcgccc tggtgggatt ccgctacgtg cagcacgatc
      841 gcagcgtcgc gaccacttac ccggcgaacc cgagcggcag ccccttcggc atcgccgcgc
      901 tctgcaacgc cgacgggcgc gtgaccatca ccatgccgca cccggagcgc tcgttccgct
      961 atctgcagaa ttcctggcgg ccgcgcgccg cgggcagcta cagcggctgg atgcggctgt
     1021 tccgcaacgc aaggcgtttc gttggttgat cagctcgccg actgcagcgg cgtgaccttg
     1081 tggctgccgg tctcgtcgag gaagaagcgg cgcaccatga tggcctgctg caggcgcccc
     1141 ttgcgcacgt aggcctcgta gaggaggtcc ttcatgactt ccagcatgat ccccgcctga
     1201 tagccatcga gcagcgggat gccggcgcgc gcgtcgagct cggcgccgaa cagcacgttg
     1261 cgcatgcgcg tgaacagatc cggggcgata tcggtgagat cgcgcaggcc gttgagctca
     1321 tcgaagagcc gcagctttcc ggcctctccc aggtccgcga acatcgcctg cgccgtgcgc
     1381 cggcacatcg aggcaaaggc gttgaaggcg ccctgcgaga agcaggcgag cgcctcacgg
     1441 aacagcagct cgacaccctc ggggatgtag gcgaagctga atttctcgcg cgcgcgctcc
     1501 acctcggt
//
LOCUS       DATLKL010000349         2831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_49037, whole genome shotgun sequence.
ACCESSION   DATLKL010000349 DATLKL010000000
VERSION     DATLKL010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49037"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VM713_04550"
     CDS             complement(<1..287)
                     /locus_tag="VM713_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVH83571.1"
                     /translation="MSQQKLSGVTGVSYQQIQKYERGTNRVGASMLWVLSEVLNVEPA
                     YFFEGLGRRGRGAAGHFGLAEAGAEPYRHDTLSDRETMELVKAYSAVGDPQV"
     gene            810..2165
                     /gene="ffh"
                     /locus_tag="VM713_04555"
     CDS             810..2165
                     /gene="ffh"
                     /locus_tag="VM713_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HVH83572.1"
                     /translation="MSSRLGDIFDRLRRRGALSETDVDAALREVRVALLEADVALPAV
                     KQLVAAIRDKAVGERVLASVTPGQMVIKLVHDELVEMLGAEAVELDLRVSPPVAILMV
                     GLQGSGKTTTTAKLALRLKTRDRKKVLMASLDTRRPAAQEQLSILGRQAEVDTLSVVP
                     GEPPLAIARRALQKARLEGYDAVLLDTAGRLHVDDALMAEAAAVREAIRPIETLLVAD
                     AMTGQDAVNVAAAFHERIGLTGIVLTRIDGDARGGAALSMRAVTGCPIKLIGVGEKLD
                     ALEPFHPQRIAGRILGMGDVVSLVEKAAETIEREEAEKLARKIEKGSFDLDDLAQQLR
                     QLRKMGGMEGVLAMLPGVAKAKRALAEHKLDPRLLKCQEAIIGSMTKAERRNPNLIHS
                     SRKRRIAAGSGTSVQDVNKLLKQFQEMSRMMKQVAKLGKKGLMRSGFPQSGLPFGPPR
                     R"
     gene            2219..2458
                     /gene="rpsP"
                     /locus_tag="VM713_04560"
                     /pseudo
     CDS             2219..2458
                     /gene="rpsP"
                     /locus_tag="VM713_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
BASE COUNT          471 a          921 c          992 g          447 t
ORIGIN      
        1 acctgcgggt cgccgacggc gctataagcc ttgaccagct ccatggtctc ccggtcgctg
       61 agcgtgtcgt gccgataggg ctcggcgccc gcctcggcga ggccgaaatg tccggccgcc
      121 cccctgccgc gacgacccaa cccctcgaag aagtaggccg gctccacgtt cagcacttcg
      181 ctcaagaccc agagcatgct cgcgccgacg cggttggtgc cgcgctcgta cttttgaatc
      241 tgctgatagc tgacgccggt gacgccgctc agcttttgct ggctcatgcc aagctcgatg
      301 cggcgctggc ggaggcgctt gccgacatgg ctgtcgatcg attctgattt tgccaccatc
      361 acccccttgt tcgacctgcc gctcaaaaga tggcgctgcc ggatctgccg ctccgctgcg
      421 cgaagccgcc gtgctgcaaa tcgctctgtg gagcggcgcg gagcgcgcta gacccgcatt
      481 tatcatcgac tcgcgtggct ttatcaaggc cgttcgcgat gccacaaacg cgacggcgta
      541 tgtgcaggag cgtgaccaag gtggtgtcgc acaacgacct ttccacttct tgtcgagcgg
      601 ccgattcgcg aattcgaagc aacttgccgc cgtatctcgc gctccctcgc cggctccgcg
      661 cgcgcttgac tcgcatcacg gcggtcccta gagtgcgccg ccatcgaagg gaagccgcag
      721 caaagcgctc ccgaaggggg tccgccagtc cgcgagtgtc gcgcactggc gcgtttgatt
      781 ttggtcgggt ggattggatg ttcgagggct tgagcagcag gcttggcgat atcttcgacc
      841 gcctgcgccg gcgcggagcc ttgagcgaga ccgacgtgga tgcggcgctg cgcgaggtgc
      901 gcgtggcgct gctcgaggct gacgtcgcgc tgcctgcggt caagcagctc gtcgcggcga
      961 tccgcgacaa ggcggtgggc gagcgggtgc tggccagtgt cacccccggc cagatggtga
     1021 tcaagctcgt gcacgacgag ctcgtcgaga tgctcggcgc cgaggccgtc gagctcgatc
     1081 tgcgtgtaag cccgcctgtg gccatcctca tggtcgggct gcagggctcg ggcaagacca
     1141 cgacgaccgc caagctggcg ctgaggctca agacgcgcga tcgcaagaaa gtgctgatgg
     1201 cttctcttga cacgcggcgg cccgcggctc aggagcagct ctcgattctc gggcggcagg
     1261 ccgaggtcga cacgctctcg gtcgtgccgg gggagccgcc gctcgcgatc gccaggcgcg
     1321 cgctgcaaaa ggcgcggctc gagggatatg acgcggtgct gctcgacacc gccggccgcc
     1381 tccacgtcga tgacgcgctg atggccgagg ccgcggccgt gcgcgaggca atcaggccga
     1441 tcgagactct gctcgtggcc gacgccatga ccggccagga tgccgtcaac gtggcggcag
     1501 ccttccacga gcgcatcggc ttgactggca tcgtgctcac gcgcatcgat ggcgatgcgc
     1561 gcgggggtgc cgcgctcagc atgcgcgcgg tcacgggctg tccgatcaag ctcattggcg
     1621 tcggcgagaa gctggacgca ctcgagccgt tccatccgca gcgaatcgcg gggcgcatcc
     1681 tcggcatggg cgatgtcgtc tcgctggtcg agaaggctgc cgagacgatc gagcgcgagg
     1741 aggccgagaa gctcgctcgg aagatcgaga aggggagctt cgatctcgac gatctggccc
     1801 agcagctgcg ccagctccgg aagatgggcg gcatggaagg cgtgctcgcc atgctgccgg
     1861 gggtggccaa ggccaagcgg gcgctcgccg agcacaagct cgaccccagg ctcttgaagt
     1921 gccaggaggc gatcatcggc tcgatgacca aggccgagcg gcgcaacccg aatctcatcc
     1981 attcctcgcg caagcgccgc attgccgcgg gctcgggcac gagcgtgcag gatgtgaaca
     2041 agctcttgaa gcagtttcag gagatgagcc ggatgatgaa gcaggttgcg aagctcggga
     2101 agaagggatt gatgcgctcc ggcttccccc agagcggcct gccgttcggg ccgccgcgcc
     2161 gctagcgggc gggcgcagcg gtcacaggtt tatcgtcgga agaggagagc acgaccccat
     2221 ggcattgaaa attcgactgg ctcgggcggg caccaagaag cgccccttct accgcatcgt
     2281 catcgccgac tcccgctcgc cgcgggacgg ccgcttcatc gagcgcgtgg gcagctacaa
     2341 cccgatggtc gccaaggacc atccggagcg cgtgatgctc aatgccgagc gcatcaagca
     2401 ctggctcggc gtcggcgcgc tgccgagcga gcgcgtggcc cgtttcctgg gcaaggcggg
     2461 cctggcaccg gtgccgccca tccccgagca gaccaagcag catctgcccc gggccaaggg
     2521 gcaggagcgg ccgaagggtg ccgacaaagc ggaagcggcg cctgccgagg gagcgaaggc
     2581 tgctccggac acggcggcta aggccgcgcc ggccaaggac gcggccgagg tgccggccag
     2641 ctagcatgcg cctcttgctt ctgcgaatgg cgaggtagtg aaaagagggc gagcgtggca
     2701 cggccaagcg atagggtctg tctcggcgtg attgcgggcg cgcatggcgt gcgtggcgag
     2761 gtccgcatcg agagcttcac ggccgacccc gccgcgctcg cgacctacgg gccgctaagc
     2821 gacgagcgcg g
//
LOCUS       DATLKL010000350         3349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_105571, whole genome shotgun sequence.
ACCESSION   DATLKL010000350 DATLKL010000000
VERSION     DATLKL010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105571"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="VM713_04565"
     CDS             complement(<1..1378)
                     /locus_tag="VM713_04565"
                     /inference="COORDINATES: protein motif:HMM:NF023689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3612 domain-containing protein"
                     /protein_id="HVH83573.1"
                     /translation="MPQLMRRGHFLGTKLRSLRKRNGLTLEELSVRCIQRDTAAAPSV
                     SYLSMIESGKRMPSPALLELLAAVFQRDPAWFLDESAEPEPAAAGAGAPVGGVARVPF
                     EPAFLFSKSLLQAAIPELLAQTGTSGRQFAHLLIRSHQEMSRNDFPDLERAAESVGER
                     RFPLGVEELLRLAKRHGLEIRWFDRKPVLARDKDREVRSMVRSFFEPPGVVYANRALQ
                     SDPARLKFDLAAHIAHKVLHGGDGLKSPHATGGEMGGSPEGGLGAAGLEPQDVLHAWR
                     DFECSFFAGALLAPRVPFRRFLARERYRVEAGARLELTPAVVMRRMTKVSPYPYWHFF
                     DAYPPGYLRAVYRGNGIPLPWGNLAQVSDPCPNWAVFRMIDGEHGGSGSQISVLRDAE
                     RSLLYCCHSRRVHDMAGNPHVLSVGVDLAPALASNGVASEPLVESILRECTHRGGEAR
                     IPKTAGQAL"
     gene            1471..3108
                     /gene="aceB"
                     /locus_tag="VM713_04570"
     CDS             1471..3108
                     /gene="aceB"
                     /locus_tag="VM713_04570"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HVH83574.1"
                     /translation="MLTYMQSGAPERAYSREPNGSRVEILGAKIERSEEVLTPLALQL
                     LASLHRRFDPRRLELLAARSGRQAEFDAGALPQFLASTAAVRAGDWRIAPVPADLLDR
                     RVEITGPVDRKMIINALNAPFSAFMADFEDSLAPTWENVIRGQVNLADAIRRTISFAD
                     AATGKVYRLAERTATLIVRPRGWHLPEKHVRINGEPVSGALFDFALFLANNHQALAAR
                     GTGPYLYLPKLESHLEARLWNDVFLAAQDALGIARGTIKATVLIETILAAFEMDEILY
                     ELREHSAGLNCGRWDYIFSFIKKFRNRPDFLLPDRASVTMERPFLKSYVDLLIRTCHR
                     RGAHAIGGMAAQIPIRDDPAANEAAMARVRADKLREARAGHDGTWIAHPGLAAIAREP
                     FDAVMHGPNQLSVLREDVHVSAPNLVAIPAGAITEEGLRNCIRVGVQYLESWLRGNGC
                     VPLYHLMEDAATAEICRAQLWQCLRHGARTSDGAAVTAVRFDRLMHAELDRIHGEVGP
                     KRLTEGVFPTAARLFEQMIKSETFDEFLTLPAYELLS"
     gene            3116..>3349
                     /locus_tag="VM713_04575"
     CDS             3116..>3349
                     /locus_tag="VM713_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83575.1"
                     /translation="MSDSTIMSLPRSLPSAEELRRVWRDSPRWRGIQRGYDAADVVRL
                     RGTVAVEHSIARLTAEKLWRSLHEKPLVNALGAL"
BASE COUNT          539 a         1165 c         1145 g          500 t
ORIGIN      
        1 cgagagcctg cccggcggtc ttggggatgc gcgcttcgcc gccacggtgc gtgcactcgc
       61 gcaggatcga ctcgaccagc ggctcgctgg ccacgccgtt ggaggccagt gccggggcca
      121 ggtccactcc caccgacagc acgtgtggat tgccggccat gtcgtgtacg cgtctggagt
      181 gacagcagta cagcagtgag cgctcggcgt cgcgcagcac cgagatctgc gagcccgagc
      241 cgccgtgctc gccgtcgatc atgcggaaca ccgcccagtt ggggcacgga tcgctcacct
      301 gcgcgaggtt gccccagggc agaggtatgc cgttcccccg gtacacggcg cgcaggtacc
      361 cgggtggata ggcgtcgaag aaatgccagt aggggtaggg agagaccttg gtcatccgcc
      421 gcatcaccac cgcgggagtg agctcgagcc tcgcgccggc ctccacccgg tagcgctcgc
      481 gtgcgaggaa gcgccggaac ggaacgcgcg gggcgagcag cgcgccggcg aagaaactac
      541 attcgaagtc gcgccaggcg tgcagcacgt cctggggctc gagccctgcg gcgccaagcc
      601 cgccctcggg gctcccgccc atctccccgc cggtggcgtg cggggacttg aggccgtcgc
      661 ccccgtgcag caccttgtga gcgatgtgcg cggcgagatc gaacttgagc cgcgccgggt
      721 cggactgcag cgctcggttc gcgtacacca ctcccggggg ctcgaaaaac gagcgcacca
      781 tgctgcgcac ttcgcgatcc ttgtcgcgcg cgaggacggg cttgcggtcg aaccagcgga
      841 tctcgagtcc gtgacgcttg gcgaggcgca gcagctcctc gacgccgagg gggaagcgcc
      901 gctcgccgac gctctccgcc gcccgctcga gatccggaaa gtcgttgcgc gacatctcct
      961 ggtgcgagcg gatgaggagg tgcgcgaact gccgtccgct cgtgcccgtc tgcgcgagca
     1021 gctccggaat cgccgcctgc agcaggctct tggagaagag gaaggcgggc tcgaagggca
     1081 cgcgcgccac gccgcctacg ggggcgcccg cgcccgcggc cgcgggctct ggctccgcgc
     1141 tctcatcgag gaaccaggcg gggtcgcgct ggaagaccgc cgccaggagc tcgagcagcg
     1201 ccggggaggg catgcgcttg ccgctctcga tcatgctgag gtacgacact gacggggctg
     1261 cggcggtgtc gcgctgaata caacgcaccg agagctcctc caacgtgagc ccgttgcgct
     1321 tgcggagcga gcgcagcttg gtgccgagga aatgtccgcg acgcatcaac tgtggcatat
     1381 ctcaaacacc cgtgccgacg ggactaaacc catggttgtg aaattaacag tgtgaagttt
     1441 ctgtagtcaa attcccttca taccccaggt atgctgacct atatgcaaag cggcgcaccg
     1501 gagcgggcct attcccgcga gccgaacggt tccagggtgg agatcctggg agcgaagatt
     1561 gagcgctcgg aggaggtttt gacgccgctc gcgctgcagc tgctcgcgag cctgcaccgg
     1621 cgcttcgatc cgcgccgact cgagctgctc gccgcgcgca gcggccgcca ggcggagttc
     1681 gacgccgggg ccctgccgca gttcctcgcg agcaccgccg cggtgcgcgc cggcgactgg
     1741 cgcatcgctc cggtgccggc ggatctcctc gaccggcgcg tcgagatcac cggtcccgtc
     1801 gaccgcaaga tgatcatcaa cgcgctcaat gcgccgttca gcgccttcat ggcggacttc
     1861 gaggattcgc tggcgccgac ctgggagaat gtgatccgcg gccaggtgaa cctcgccgac
     1921 gccatccgcc gcacgatcag cttcgcggac gccgcgaccg gcaaggtcta tcggctcgcc
     1981 gagcgcaccg cgacgctcat cgtgcggcca cgcggctggc acctgcccga gaagcacgtg
     2041 cgcataaacg gcgagccggt gtcgggcgca ctgttcgact tcgcgctgtt tctcgccaac
     2101 aaccaccagg ccctcgcggc gcgcggcacc ggcccgtacc tttatctccc gaagctcgag
     2161 agccacctgg aagcgcggct gtggaacgac gtgttcctcg cggcgcagga tgcgctcggc
     2221 atagcgcgcg gcaccatcaa ggcgacggtg ctgatcgaga ccatcctcgc ggcgttcgag
     2281 atggacgaga tcctctacga gctgcgtgag cactccgccg ggctcaactg cggccgctgg
     2341 gattacatct tcagcttcat caagaagttc cgaaaccggc cggactttct gctcccggac
     2401 cgcgccagcg tgaccatgga gcggcccttc ctcaagtcct acgtcgacct cctcatccgc
     2461 acctgtcatc gccgcggcgc gcatgcgatc gggggcatgg cggcgcagat accgatccgc
     2521 gacgatccgg ccgccaacga ggcggcgatg gcgcgcgtgc gggccgacaa gctgcgcgag
     2581 gcgcgcgccg gacacgacgg cacctggatc gcgcacccgg gactcgcggc gatcgcgcgc
     2641 gagccgttcg atgcggtgat gcatgggccc aaccagctgt cggtgctgcg cgaggacgtg
     2701 cacgtgagcg cgcccaacct ggtggcaatc ccggccggcg cgatcaccga ggagggcctg
     2761 cgcaactgca tccgtgtcgg cgtgcagtat ctcgaatcgt ggctgcgcgg caacggctgc
     2821 gtgccgctct atcacctcat ggaggacgcg gccaccgccg aaatctgccg ggcgcagctg
     2881 tggcaatgtc tgcgccacgg tgcgcgcacc agcgacggcg ccgcggtcac ggcggtgcgc
     2941 ttcgatcggc tgatgcatgc cgagctcgac cgcatccacg gcgaagtggg gccgaagcgc
     3001 ctcaccgagg gcgtgttccc gaccgccgcg cgcctcttcg aacagatgat caagtccgag
     3061 accttcgatg agttcctgac gctgcccgcc tacgagctgc tgagctgagg accccatgag
     3121 cgatagcacc atcatgagtc tgccccgttc actccccagc gccgaggagc tgcggcgcgt
     3181 gtggcgcgac agcccccgct ggcgcggcat ccagcgcggc tacgacgctg ccgacgtcgt
     3241 gcgcctgcgc ggcacggtcg cggtcgagca ctcgatcgcg cggctcaccg ccgagaagct
     3301 ctggcgctcg ctgcacgaga agcccctggt gaacgcgctc ggcgcgctc
//
LOCUS       DATLKL010000351         1533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_116951, whole genome shotgun sequence.
ACCESSION   DATLKL010000351 DATLKL010000000
VERSION     DATLKL010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116951"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VM713_04580"
     CDS             <1..294
                     /locus_tag="VM713_04580"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HVH83576.1"
                     /translation="YASFREFYPFYLGEHTNRTSRRLHVSGTLIALVLAAGALVSGHL
                     ALLWAVPLAGYAFAWVGHYFFEKNRPATFTYPLYSLGGDFRMLYEVLTGRMRW"
     gene            297..>1533
                     /gene="gshA"
                     /locus_tag="VM713_04585"
     CDS             297..>1533
                     /gene="gshA"
                     /locus_tag="VM713_04585"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HVH83577.1"
                     /translation="MSSAAIDRVFERRLASLVNSGEPQILQGGRKGVEKESLRVTPEG
                     HLAHTPHPRALGSALTSEHITTDFSESLIELVTPAFTHTWELLQYLLDLHQFVYRHLG
                     DELLWATSMPCSIDGDADIPLAQYGSSHVGRMKTVYRNGLGLRYGRMMQAISGVHFNY
                     SFPLPFWEAYAALRGARSSGSEFVSASYFDLLRNYRRHGWLVLYLFGVSPVVCKSFLR
                     GRDLKLQELGMGTAYEPHATSLRMSDVGYRNRTQAGLTVSVNDLQEYVRDLQRAITTP
                     HPPYEALGVKVNGEWRQLNANVLQIENEYYSFIRPKRVARSGERPTKALKRAGVEYVE
                     VRALDVSAFDPVGVNQNKLRFLEAFLALCLLKESTPIGLSEQAALDANHQAVARHGRE
                     PGLTLERDGRRAPMREWAGE"
BASE COUNT          238 a          527 c          514 g          254 t
ORIGIN      
        1 tacgcgagct ttcgcgagtt ctatccgttc tatctcggcg agcacaccaa ccgcacgtcg
       61 cgccgcctgc acgtgagcgg cacgctcatc gcgctcgtgc tcgccgccgg ggcgctcgtg
      121 agcgggcact tggcgctgct ctgggcggtg ccgctcgccg gctacgcctt cgcatgggtc
      181 gggcactact tcttcgagaa gaaccggcct gcgaccttca cgtatccgct ctacagcctc
      241 ggcggggact tccgcatgct ctacgaggtg ctgacgggcc ggatgcgctg gtagccatga
      301 gcagcgcggc catcgatcgc gtcttcgagc ggcgcctcgc ctccctcgtc aacagcggcg
      361 agccgcagat cctgcagggc ggccgtaaag gggtcgagaa ggagtcgctg cgggtgacgc
      421 cggagggcca tctcgcgcac acgccgcacc cgcgggcgct cggctcggcg ctcacgagcg
      481 agcacataac caccgatttc tccgagtcgc tgatcgagct ggtgacgccc gccttcacgc
      541 acacctggga gctgctgcag tacctgctcg acctgcacca gttcgtctac cgccacctgg
      601 gggacgagct cctgtgggcg accagcatgc cgtgctccat cgacggcgat gccgacatcc
      661 cgctcgcgca gtacggcagc tcgcacgtcg ggcgcatgaa gaccgtgtac cgcaacggcc
      721 tggggctgcg ttacgggcgc atgatgcagg cgatctcggg agtgcatttc aactattcgt
      781 tcccgctgcc gttctgggag gcgtacgcgg cgctgcgcgg cgcgcgctcc tcgggaagcg
      841 agttcgtctc ggcgagctac ttcgatctgc tgcgcaacta tcgccgccac ggctggctgg
      901 tgctgtacct cttcggcgtc tcaccggtgg tgtgcaagtc gttcctgcgc gggcgcgacc
      961 ttaagttgca ggagctcggc atgggcacgg cctacgagcc ccatgccacg agcctgcgca
     1021 tgagcgatgt gggttaccgc aaccgcaccc aggcagggct caccgtctcg gtgaatgatc
     1081 tgcaggagta cgtgcgcgac ctgcagcgcg cgatcacgac accgcacccg ccctatgagg
     1141 cgctcggcgt gaaggtgaac ggcgagtggc gccagctcaa cgccaacgtc ctgcagatcg
     1201 agaacgagta ctacagcttc atccgcccca agcgggtggc gcgctcgggc gagcggccca
     1261 ccaaggccct gaagcgcgcc ggcgtcgagt acgtcgaggt gcgcgctctc gatgtcagcg
     1321 ccttcgaccc ggtgggggtg aatcagaaca agctgcgttt cctcgaggcg ttcctcgccc
     1381 tgtgcctgct caaggagagc acgcccatcg gcctcagcga gcaggccgcg ctcgatgcga
     1441 accatcaggc ggtggcgcgc cacggccgcg agcccggact cacgctcgag cgtgatggcc
     1501 gcagggcgcc gatgcgcgag tgggcgggcg agc
//
LOCUS       DATLKL010000352         2277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_207021, whole genome shotgun sequence.
ACCESSION   DATLKL010000352 DATLKL010000000
VERSION     DATLKL010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207021"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1563
                     /locus_tag="VM713_04590"
     CDS             142..1563
                     /locus_tag="VM713_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HVH83578.1"
                     /translation="MARSDAESSLSAPDAALFPPTARRQELDDHLTRALALALERMRA
                     GPATPTIDIEAFNRELAGFDFAAGRPLTELLEWAIGVLEHGVTHQTHPRYFGLFNPAP
                     TFPAQCADRIVNSFNPQVASATTSPAAVALEAHVIRSVAQRVGFPPGTGGHFTSGGSE
                     ANYTGMLCALTRAHLAFASEGARAFAGQPVFYTSRECHGSWVKIAHQAGIGRSAAHLV
                     ATDGRGRMSAAALEQAISQDLAAGRVPFLIVASAGTSNAGMIDPLHECAELAARHRLW
                     YHVDAAWGGSIVASERLRAAVAGLERADSATIDAHKWLATTMACGMFIVRDPTALTAA
                     FQVAASYMPSRDPSLDPYTNSVQWSRRFLGLRLFLSLAAAGWAGHAAHIERAVAQAAW
                     IRSELESRGWSVVNDSPLAVLNVVPPAPVGEARSIVARVLKSGRAWVSLARFEEQDVV
                     RICITHGETSAEDLAILVQALHG"
     gene            complement(1569..>2277)
                     /locus_tag="VM713_04595"
     CDS             complement(1569..>2277)
                     /locus_tag="VM713_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83579.1"
                     /translation="PSLAVRTRGSIKDTFGQVATSAAVVQGQPITAYVPAGVTGTTVV
                     NLLPLGQVPTAASAAYRFNADNLTISIPTSELPAGVYGAWLRTGTAPPAGSPMMVRIL
                     PPPHVSPSAPGVEIVGAEATGGNSADVELREGTDAVVRYCLPAVSDLADGWIGVFPAG
                     TPLNNMTKKAARTIGIWLHTPGGGGQPAPCGEAEAYTSELTPNTTYQVLLFMTASNGT
                     KQIGSTAEFTVTPALPH"
BASE COUNT          320 a          779 c          805 g          373 t
ORIGIN      
        1 cgaagcgtac aagcgcggag cgatgctgcg gctgtcgggg gcgaacatca ttatctcgcc
       61 cgccctcacg atcgagcggg cgcagatcga cctgctgtgc gatgcgctcg aaggcgcgtt
      121 cggcgtcttc gagggctgag catggcccgc tccgacgcag agtcctcgct cagcgccccg
      181 gacgcggcgc tgtttccgcc aacagcgcgg cggcaggagc tcgatgacca cctgacgcgc
      241 gcgctggcgc tcgcgcttga gcgtatgcgc gcgggaccgg ccacgccgac catcgacatc
      301 gaggccttca accgcgagct ggccggcttc gatttcgcag ccggacggcc gctgactgag
      361 ctgctcgagt gggcaatcgg cgtactcgag cacggcgtca cgcaccagac gcacccgcgc
      421 tacttcgggc tcttcaaccc cgcacccacg ttccccgcgc agtgcgccga ccgcatcgtg
      481 aattcgttca atccacaggt cgccagcgcc acaacctcgc cggccgccgt ggcgctcgag
      541 gcacacgtga tccgcagtgt cgcgcagcgg gtaggattcc cgccgggcac tggcggccac
      601 ttcacctcgg gtgggtcaga ggccaattac accgggatgt tgtgcgcgct gacccgcgcg
      661 catctggcgt tcgcgtccga gggcgcgcgc gcgttcgcgg ggcagccggt gttctacacc
      721 tcgagggagt gccacggcag ctgggtgaag atcgcgcatc aggcgggcat cggccgttcc
      781 gccgcgcacc tggtggccac cgatggccgc gggcgcatgt cggcggcggc cctcgaacag
      841 gcgatctccc aggatctcgc cgccggccgc gtgcctttcc tgatcgtcgc ctcggcggga
      901 acgagcaacg ccggcatgat tgatcccctg catgaatgtg ccgagctcgc cgcgcgccac
      961 cggctctggt accacgtcga tgcggcctgg ggcggttcca ttgtggcttc cgagcggctg
     1021 cgcgcggcgg tcgccgggct ggagcgcgcc gattcggcga ctatcgacgc gcacaagtgg
     1081 ctcgcgacca cgatggcgtg cggcatgttc atcgtccgcg atcccacggc gctgacggcc
     1141 gcgtttcagg tagcggcgag ctatatgccc tcgcgcgacc cgtcgctcga tccgtacacc
     1201 aattccgtgc aatggtcgcg ccgctttctc gggctgcggc tgttcctgtc gctggcggcg
     1261 gccggctggg caggccatgc ggcccacatc gagcgcgcgg tcgcacaagc ggcttggatt
     1321 cgcagcgagc tcgaaagccg gggctggtcg gtggtcaatg actcgccgct cgctgtgctc
     1381 aacgtcgtac cgcccgcgcc ggtgggtgag gcgcgctcga tcgtcgcacg cgtgctgaag
     1441 tcaggccgcg cctgggtgtc gctcgcgcgt ttcgaggagc aggacgtggt gcgtatctgc
     1501 atcacgcacg gcgagacgag cgcggaggat ctggccatac tggtgcaggc cttgcacggg
     1561 tgatcgtgct agtgcggcaa ggccggtgtt accgtgaatt cggccgtgct accgatctgc
     1621 ttggtgccgt tcgaggcggt catgaacagc agcacctgat aagtggtgtt cggggtgagc
     1681 tctgaggtgt aggcctcggc ctcaccgcag ggcgccggct gtcctccgcc ccccggcgtg
     1741 tggagccaga tgccgatggt ccgcgcggcc ttcttggtca tgttgttgag cggcgtgccg
     1801 gccggaaaga cgccgatcca gccgtccgca agatcagaga cggcgggcag gcaatagcgc
     1861 acgactgcgt cggtcccttc ccgcagctcg acgtcggccg aatttccgcc ggtcgcctcg
     1921 gccccgacga tctcgacgcc gggcgcgctc gggctgacgt gcggcggcgg cagaatcctc
     1981 accatcatcg gggagccggc ggggggcgcg gtgccggtcc gcagccaggc gccgtacacc
     2041 ccggccggga gctcgctcgt cgggatcgag atcgtcagat tgtccgcgtt gaaccggtaa
     2101 gctgccgagg cggcggtcgg cacctgcccc aagggcagaa ggttgacaac cgtggtgccg
     2161 gtgacgccgg ccggcacata cgccgtgatc ggctggccct gcacgacggc ggcggacgtg
     2221 gccacctgcc cgaacgtgtc cttgatgctg ccgcgcgtgc gcaccgcgag gctcggc
//
LOCUS       DATLKL010000353         1669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_71926, whole genome shotgun sequence.
ACCESSION   DATLKL010000353 DATLKL010000000
VERSION     DATLKL010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71926"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VM713_04600"
     CDS             complement(<1..1068)
                     /locus_tag="VM713_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HVH83580.1"
                     /translation="MAHADFVHLRVHTAYSLSEGAIKIKPLIELCRQHAMPAVAITDT
                     GNLFGALEFAQAAEAAGVQPIIGCQVSIRREEGEGRGSGQTLLKTGAEPLVLLVKDEA
                     GYRSLLKLVSKSYLETPTGEAPQVALADLATLEQGLIAFTGGSAGAVGRLLAEGQASA
                     AEALLLRLAAIFDGRLYVELQRHGLAPEAQLEAPLLDLAYRHALPLVATNEVFFADER
                     MFEAHDALICIAEQTYISEVNRRRLTPEHRFKSAAEMRALFADLPEAVDNTLTIAQRC
                     SFYPEPRRPILPAYPTAGGRDEASELRAQAEEGLERRLDVQIFKPDMDGEARESVARP
                     YRERLHYELGVISQMGFAGYFL"
     gene            complement(1149..>1669)
                     /locus_tag="VM713_04605"
     CDS             complement(1149..>1669)
                     /locus_tag="VM713_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVH83581.1"
                     /translation="LGGLDVPSEGSVAVNGKFLNEVSESERGDIRNRTLGFVYQFHHL
                     LPEFSALENVMLPQMVAGSSPKAARERGRQILAMVGLEKRAGHRPARLSGGEQQRVAI
                     ARAIANGPRILLADEPTGNLDHHTADTVFAQLLGLVREAGLAALVATHNLELARRMDR
                     TLELRDGAVVPL"
BASE COUNT          271 a          552 c          573 g          273 t
ORIGIN      
        1 gaggaaatac ccggcaaagc ccatctggga gatgacgccg agctcgtaat ggagccgctc
       61 acggtagggc cgcgctacgc tctcgcgcgc ttctccatcc atgtccggct tgaatatctg
      121 gacgtcgagg cgccgctcga gcccctcctc cgcctgggcc ctgagctcgc tcgcctcgtc
      181 tcgtccgccc gcggtcggat aggccgggag gatcggtctg cgcggctcgg gatagaatga
      241 gcagcgttgg gcgatggtga gcgtgttgtc gaccgcctcc ggaagatcgg cgaagagggc
      301 gcgcatctcg gccgccgact tgaatcggtg ctcgggtgtc agccgacgcc ggttgacctc
      361 agagatgtag gtctgctcgg caatgcagat cagcgcgtca tgggcctcga acatgcgctc
      421 gtcggcaaaa aacacctcgt tggtggcgac taacggcagg gcatggcggt aagcaaggtc
      481 gaggagtggc gcctcgagct gcgcctccgg cgcgagccca tggcgctgga gctcgacgta
      541 cagccggccg tcgaagatcg ctgcaagacg cagcagcagg gcctccgccg ccgacgcctg
      601 cccctccgcg agcaacctgc cgacggcgcc tgccgatccg ccggtgaagg cgatcaggcc
      661 ttgttcgagc gtcgcgagat cggccagagc cacctgcggc gcttcgccgg tcggggtctc
      721 gaggtaggac ttgctgacga gcttcagaag cgaccggtag cccgcctcgt ccttgaccag
      781 caggaccagg ggttccgccc ccgtcttgag cagagtctgc ccgctgccgc gtccttcgcc
      841 ctcctcacgg cggatcgaca cttggcagcc gatgatcggc tggacaccgg ccgcctcggc
      901 cgcctgcgcg aattcaaggg cgccgaacag gttgcccgtg tcggtgatgg cgacggccgg
      961 catagcgtgc tgccggcaaa gctcgatcag cggcttgatc ttgatcgccc cttccgagag
     1021 cgagtaggcg gtgtggaccc ggagatggac gaaatcagcg tgcgccattg cctcactcag
     1081 gagtgatcga gcacaggatc gcacagagcg cggcgggtgt cagcgcgaat gagtcggggc
     1141 tcagcgcctt acagcggcac gactgccccg tccctgagct cgagcgtgcg gtccatgcgc
     1201 ctggccagct ccaggttgtg ggtggcgacc agcgccgcga gcccggcctc tcgaacgagc
     1261 ccgaggagct gggcgaacac ggtatcggcc gtgtggtggt ccagatttcc cgtcggctcg
     1321 tcggccagga gaatccgcgg gccattcgcg atcgcgcgcg cgatggcgac ccgttgctgc
     1381 tcgccgcccg agagccgagc cgggcggtgg cctgcgcgct tctcgaggcc aaccatggcg
     1441 aggatctgac gcccgcgctc gcgcgcggcc ttgggcgagc tgccggccac catctgaggc
     1501 aacatgacgt tctcgagcgc cgagaactcg ggcagcagat ggtggaactg gtagacgaag
     1561 ccgagggtgc ggttgcggat gtcgccgcgc tcgctttccg acacttcatt caggaatttt
     1621 ccattgacgg ccaccgatcc ctcgctcgga acatccaggc cccccagga
//
LOCUS       DATLKL010000354         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_184634, whole genome shotgun sequence.
ACCESSION   DATLKL010000354 DATLKL010000000
VERSION     DATLKL010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184634"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1870
                     /locus_tag="VM713_04610"
     CDS             302..1870
                     /locus_tag="VM713_04610"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HVH83582.1"
                     /translation="MTTNPDRVLIFDTTLRDGEQSPGCSMTQPEKLRVARALAELGVD
                     VIEAGFPAASRGDFESVQAVAREVQGPIICGLARCNPEDIDLAARALKDASRRRIHVF
                     LATSAIHRQYKLNMAQDEILRAAAAGVTRARELADDVEFSPEDASRTELEFLAQVVET
                     AIAAGASTVNIPDTVGYTVPDEFAELFRYLRKNVRGIDGIRLSVHCHDDLGMAVANSL
                     TAVVAGARQIECTINGIGERAGNCSLEEVVMALKTRAAFFNVTTGIHTQRLYPTSRLV
                     SSITGMPVPRNKAVVGENAFAHEAGIHQHGMLKHYSTYEILRPEDVGLSRSHLVLGKH
                     SGRHALRERVQALGFELGEEEFNQLFEEFKALADRKKELYDGDIEALVLRAEGSAQGP
                     WTLTELTTETRSTGAARALVVLTHTDGRSVEHGASGDGPVDAAFKAIEAATGIRVVLR
                     KFEVKAVSEGEDAQGEARVYVEYNRRTYRGASVSTNIVESGTRAFLEVINRIELAQAG
                     TRARAATPAVPAAV"
     gene            1886..>2590
                     /locus_tag="VM713_04615"
     CDS             1886..>2590
                     /locus_tag="VM713_04615"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HVH83583.1"
                     /translation="MPIPATQFIWFNGKLVPWEKATVHVLSHALHYGSSVFEGERAYE
                     TPRGVAIFRLRDHTRRLFDSAKIYRIAMPYSPEQINDACRQVISANGLKRGAYIRPVV
                     FRGYGEIGVAPKIDPPTDVAIAAWEWGKYLGAEDEDAGVDACVSSWNRVAPNTLPALA
                     KAGGNYLSSQLIGAEARRLGFAEGIGLSPDGTLSEGSGENLFLVKDGVLLTPALAHSV
                     LGGLTRDTVIRLARERG"
BASE COUNT          431 a          875 c          897 g          387 t
ORIGIN      
        1 gttcgcgcgc caatggatca aggagaacga gaccggcaag gcgaactacc agaagctgct
       61 ggccgccgac atcgaccggc agatagagaa agtcggcgcc agccttcggg ctcggatgcc
      121 atggctcaat gaacggcgag cgtgagatga aggccgctgc gatgagatcg acactttcgg
      181 gggcgtgcgc gccagggatg gcgcgcccgc agcctccagg gacggattta cggcgtcccc
      241 gaaagtgtct gtctcggcgc agcggtactc atcgctctga cgacgttttg aggtatcgca
      301 tatgacgacc aatcccgacc gcgttctgat cttcgacacc acgctgcgcg atggggagca
      361 gtcgccgggc tgcagcatga cgcagccgga gaagctgcgc gtcgcacgcg cgctcgccga
      421 gctcggcgtc gacgtgatcg aagcgggctt tcccgccgct tcgcgcggcg acttcgagag
      481 cgtgcaggcc gtggcgcgcg aggtgcaggg gccgatcatc tgcggcctgg cgcgctgtaa
      541 tcccgaggac atcgacctcg ccgcacgcgc gctcaaagac gcgtcgcgcc ggcgcatcca
      601 cgtgttcctc gccaccagcg ccattcaccg gcagtacaag ctcaacatgg cgcaggacga
      661 gatcctgcgc gccgccgcgg ccggcgtgac ccgcgcgcgt gagctcgccg acgatgtcga
      721 gttctcgccc gaggatgcct cgcgcaccga gctcgagttc ctcgcccagg tggtcgagac
      781 cgcgatcgct gccggtgcca gcacggtcaa cattcccgac acggtggggt acacggtgcc
      841 ggatgagttc gcggagctgt tccgctatct gcgcaagaac gtgcgcggca tcgacggcat
      901 ccgcctctcg gtgcattgcc acgacgatct cggcatggcg gtcgccaaca gcctgaccgc
      961 ggtggtcgcc ggagcgcgcc agatcgagtg caccatcaac ggcatcggag agcgggccgg
     1021 caactgctcg ctcgaggagg tggtgatggc cttgaagacc cgcgccgcgt tcttcaacgt
     1081 gaccacgggc attcacaccc agcgcctcta tccgacctcg cgcctggtct cgagcatcac
     1141 cggcatgccg gtgccacgca acaaggcggt ggtcggcgag aacgccttcg cgcacgaagc
     1201 cggcatccac cagcacggga tgctcaagca ctactcgact tacgagatcc tgcgtcctga
     1261 ggacgtcggg ctgtcgcgca gccacctggt cctcggcaag cacagcgggc gccatgcgct
     1321 gcgcgagcgg gtgcaggcgc tcggcttcga gctcggcgag gaggagttca accagctgtt
     1381 cgaagagttc aaggccctgg ccgaccgcaa gaaggagctc tacgacggcg acatcgaggc
     1441 gctggtgctg cgcgccgagg gctcggctca gggaccctgg accctcaccg agctcaccac
     1501 cgagacgcgc agcacgggtg cggcgcgcgc gctggtggtg ctcacgcata ccgatgggcg
     1561 cagcgtcgag cacggggcga gcggtgacgg gccggtcgat gccgccttca aggcgatcga
     1621 agcggccacc ggcatccgcg tggtgctgcg caagttcgag gtcaaggcgg tctccgaggg
     1681 tgaggatgcg cagggcgagg cgcgggtgta cgtggagtac aatcgccgca cctaccgggg
     1741 cgccagcgtg agcacgaaca tcgtcgagtc cggcactcgc gcgttcctcg aggtgatcaa
     1801 tcgcatcgag ctggcgcagg ctgggacgcg ggcgcgcgcg gcgacgccgg cggtgcccgc
     1861 cgcggtctga cctacaggag ctcccatgcc tattcccgcc acgcagttca tctggttcaa
     1921 cggcaagctc gtgccatggg agaaggcgac ggtgcacgtg ctgtcccacg cgctccacta
     1981 cggctcttcc gtttttgagg gcgagcgcgc ctacgagacg ccgcgcgggg tggccatctt
     2041 ccgcctgcgc gaccatacac ggcggctgtt cgattcggcc aagatctacc gcatcgccat
     2101 gccctactcg cccgagcaga tcaacgacgc ctgccgccag gtgatctcgg ccaacggcct
     2161 caagcgcggc gcctacatcc gcccggtcgt gttccgcggt tacggcgaga tcggcgtggc
     2221 gccgaagatc gacccgccca cggacgtcgc gatcgcggcc tgggaatggg gcaagtacct
     2281 cggcgccgag gacgaggacg ccggggtgga cgcctgcgtg tcctcatgga accgggtggc
     2341 ccccaacacg ctgccggcgc tcgccaaggc gggcggcaac tacctctcga gccagctgat
     2401 cggcgcggag gcgcgccgcc tcggcttcgc cgagggcatc ggactgtcgc ccgacgggac
     2461 cctgagcgag ggctcgggcg agaacctctt tctggtgaag gacggggtgc tgcttacccc
     2521 ggcgctcgcg cattcggtgc tgggcggcct gacccgcgac acggtgattc gcctcgcgcg
     2581 cgagcgcggc
//
LOCUS       DATLKL010000355         1997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_4037, whole genome shotgun sequence.
ACCESSION   DATLKL010000355 DATLKL010000000
VERSION     DATLKL010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4037"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="VM713_04620"
     CDS             <1..1807
                     /locus_tag="VM713_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVH83584.1"
                     /translation="QELAGFRELRSRLFNKRGDGTTARATRRSTVSFDAVFCEQAEAA
                     VTAVREALAADRPFAVVFLDVRMPPGPDGVWAATQIRELDPAIEIVICTAYSDTDPCE
                     IGGIVPPEDKLSYLQKPFHPHEVRQMTVALGAKWRAERRIVKLAYFDTLTGLPNREQS
                     RTRLESALVAARESQRTLAVLYLDLDNFKRVNDTLGHAVGDELLCVVAKRLRGSLRYG
                     DPRSEHGSTARPGDLARLGGDEFMVLLPHLHSSGDAGAVADRIIDALREPMQLAQNTL
                     VVTPSVGIALYPQDGTDADTLLRNADLAMYFAKRRSPGTHAFFDVSMNASALHRFTIE
                     GRLRGALERNELFLNYQPQFDVRTGAISGLEALLRWTSAELGVVTPAEFIPIAEETGL
                     IHPIGRWVLRSACRQARAWCTEGLPLQRIAVNVSGRQFALPGFSEAVAEILRETGLEP
                     SILELEITESVVTADEEWAERAINQLKQIGVSIAIDDFGTGYSSFGRLRHFAVDRLKI
                     DRSFVTSITECGDDRAIAGAIIAMSRSLHVNVTAEGVENFPQLAFLQEQECQDAQGFL
                     LSRPLQADAARALLRRMQDLADYSRTQRLKALMNK"
BASE COUNT          325 a          667 c          663 g          342 t
ORIGIN      
        1 acaggaactg gccggcttcc gcgagctgcg cagtcgactg ttcaacaaac gcggcgacgg
       61 tacgactgcg cgcgcgacgc ggcgtagcac cgtgtccttc gatgcggtct tctgcgagca
      121 ggcggaggcc gcggtgactg ccgtgcggga ggcactggcc gccgatcggc cgttcgcggt
      181 agtgttcctg gacgtgcgca tgccgccggg tcccgacggg gtgtgggcgg cgacccagat
      241 ccgtgagctc gatccggcga tagagatcgt catctgcacg gcgtactccg ataccgatcc
      301 ctgcgagatc ggcggcatcg tgccccccga ggacaagctc tcgtacctgc agaagccgtt
      361 ccatccgcac gaggtgcgcc agatgacagt ggcgctcggc gccaagtggc gcgcggagcg
      421 gcgcatcgtg aagctcgcct acttcgacac gctcaccggg ctgcccaacc gcgagcagtc
      481 ccgcacccgg ctcgagagcg cactggtcgc ggcccgggag agccagcgaa ctctggcggt
      541 gctgtacctg gatctcgata acttcaagcg ggtcaacgat accctcggcc acgccgtcgg
      601 tgacgagctc ttatgcgtgg tagccaagcg cctgcgcggc tcgctgcgct acggcgaccc
      661 ccgcagcgag cacggctcca ctgcccgtcc cggcgacctg gcgcgcctcg gaggcgacga
      721 gttcatggtg ctgctaccac acctgcacag ctcgggagac gctggtgcgg tcgccgaccg
      781 aatcatcgac gcgctgcgcg agccgatgca gctggcacag aatactctgg tcgtcacccc
      841 gagcgtcggc atcgctctgt acccgcagga cggaacggac gccgacacgc tgctgcgcaa
      901 cgccgatctc gccatgtact tcgccaagcg ccgcagtccc gggacgcatg cgttcttcga
      961 cgtgtcgatg aacgcaagcg cgctgcaccg cttcaccatc gagggccggc tgcgcggggc
     1021 gctggagcgc aacgagctct tcctgaatta ccagccacag ttcgacgtgc gcaccggcgc
     1081 catctccggt ctcgaggcgc tgctgcgctg gacgagcgcg gagcttggcg tggtaactcc
     1141 cgccgaattc atcccgattg ccgaggagac cgggctcatt catccgatcg gccgctgggt
     1201 gctgcggtcg gcctgccggc aggcgcgggc ctggtgcact gagggcctgc cgctgcagcg
     1261 catcgcggtc aacgtctcgg ggcgccagtt cgcgctgccc gggttcagtg aggcggtggc
     1321 cgagatcctg cgcgaaaccg gactcgagcc ctccatactc gaactcgaga tcaccgaatc
     1381 ggttgtcacg gccgacgagg aatgggcgga aagggccatc aatcagctca agcagatcgg
     1441 tgtgtcgatt gccatcgacg acttcggcac cggttactcg agcttcggcc gcctgcgcca
     1501 tttcgccgtc gaccggttga agatcgaccg ctccttcgtc accagcatca ccgaatgcgg
     1561 cgacgaccgt gctatcgccg gcgccatcat cgccatgtcg cgctcattgc acgtcaacgt
     1621 gaccgccgag ggcgtggaga acttcccgca gctggcgttc ctgcaggagc aggagtgcca
     1681 ggacgcgcag ggcttccttc tcagccggcc gctgcaggcg gatgcggcgc gcgcgctgct
     1741 gcgtcggatg caggacctgg ccgactactc gcgcacacag cgtttgaagg cgctcatgaa
     1801 taagtgattt ggtgcggagc tacgcgcggg gctctggatg cttacctgga tgcttcaagg
     1861 cgtagctgca gccccgctga cttgccttgt gtagtgactc agtggtgact ttcgtccccg
     1921 aacctgattg agcgggcgcc aggttgttga ttaattggcg cgcccggaaa gattgctcgg
     1981 ccgctgacgc ggcctcg
//
LOCUS       DATLKL010000356         2437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_105649, whole genome shotgun sequence.
ACCESSION   DATLKL010000356 DATLKL010000000
VERSION     DATLKL010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105649"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VM713_04625"
     CDS             <1..743
                     /locus_tag="VM713_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HVH83585.1"
                     /translation="GKALVAYALFVTAVVFNVAAIANNNLQDLKTGQLVESTPSKQQW
                     ALVIGVIASAVVIPPVLTKVNKAYGFAGAPGAGAHALPAPQAGLISALRQAVIQSDPE
                     KWQLMGVGALVGAGIIAVDFLLSKTTRSLRLPPLAVGLGIYLPTASTLMVTVGALVGW
                     WFDRRADRSSKPNATKQLGVLLASGLIVGESVLAVVFTALVAFTNNPFPIGVVGDSFE
                     TASEVLGGIAFVALIWWVYRWAERLQAR"
     gene            complement(751..1680)
                     /locus_tag="VM713_04630"
     CDS             complement(751..1680)
                     /locus_tag="VM713_04630"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HVH83586.1"
                     /translation="MPSSGVLAAIGNTPLIELQRVPGAGAARLVVKLESANPTGSMKD
                     RVARAMIERAAADGRLRPRDTVVEYTAGTTGISLALVCAALGFKAHFVFSDAFSPDKR
                     HTMRAYGAEITDVEAVGGAITEQLIRALIAKADEISRRPGHWYCDQLNNRDGEAGYHA
                     LGEELWQQSGGRVDAFVQSVGTAHSIHGSAHALRLHNPRLRVIAVEPAESPVLSGGAP
                     GAHHIDGIGIGFVPPLWRRAEVDEILTVPTADARAMARRLAREEGVFAGTSTGANLVA
                     ALAVAQRLGPKATVATIAVDSGLRYLSTDVFRG"
     gene            complement(1703..2074)
                     /locus_tag="VM713_04635"
     CDS             complement(1703..2074)
                     /locus_tag="VM713_04635"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HVH83587.1"
                     /translation="MTGFLLRALLSAFGLWLASRWVHGVRIDDVPTLLLAGLLLGLVN
                     AVVRPVAFILTLPLTIVTLGLFLLVLNAAMVALVAWILPGFHLYGGFRAALLTWIIVW
                     LTGWLGSALIGRHGFERLPQR"
     gene            complement(2071..>2437)
                     /locus_tag="VM713_04640"
     CDS             complement(2071..>2437)
                     /locus_tag="VM713_04640"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HVH83588.1"
                     /translation="ESLARWCSPEAELQPRQGGLFRARVDRLTELEAHIDVFEKGRRV
                     RLIYLPNPAWPRTESVVTDDLLIEAVPGGTVVRLLGSGLPGEPEWDTQYWRLRTSWLA
                     ALNRLKVLVEGQRGAGAAG"
BASE COUNT          367 a          862 c          861 g          347 t
ORIGIN      
        1 tgggcaaggc actcgtggcc tacgcactgt tcgtcaccgc cgtggtgttc aacgtggcgg
       61 ccattgccaa caacaacctg caggacttga agaccgggca gctggtggaa tccacgccct
      121 cgaaacaaca gtgggcgctg gtgatcggcg tcatcgcgag cgcggtcgtg atcccgccgg
      181 tacttaccaa ggtgaacaag gcctacggct ttgcgggcgc ccccggcgcg ggagcccacg
      241 ctctgcccgc gccccaggcg ggcctcatct ccgccctccg gcaggcggtg atccagagcg
      301 atccggagaa gtggcagctg atgggcgtgg gggccctcgt cggcgccggc atcatcgcgg
      361 tcgacttcct gctctcgaag accacgcgct ccctgcgcct gccgcccctt gcggtgggtc
      421 tcgggatcta cctgccgacc gcctcgacgc tgatggtcac cgtgggcgca ctcgtcggct
      481 ggtggttcga ccggcgcgcc gaccggagca gcaagcccaa cgccacgaag cagctcgggg
      541 tgctgctcgc ttcgggcctc atcgtcggcg agagtgtgct cgcggtcgtc ttcacggcgc
      601 tcgtggcctt caccaacaac cccttcccca tcggagtggt gggagactcg ttcgagaccg
      661 cctcagaggt cctcggcggg attgccttcg tcgcgctgat ctggtgggtg taccgctggg
      721 cggaaaggct gcaggcgcgc tgacgcggct tcagccgcgg aagacgtcgg tgctgagata
      781 acgcagcccc gaatccaccg cgatcgtcgc cacggtggcc ttggggccca agcgctgcgc
      841 cacggcgagc gccgccacga ggttagcgcc ggtcgaggtg ccggcgaaca cgccctcctc
      901 gcgcgccagg cggcgcgcca tcgccctggc atccgcggtc ggcacggtga ggatctcgtc
      961 gacttccgcg cgccgccaga gcggcggcac gaagccgatc ccgatgccgt cgatgtggtg
     1021 tgcccccgga gcgccccccg agagcaccgg cgattcggcc ggctcgaccg cgatcactct
     1081 gaggcgcggg ttgtgaaggc gcagcgcgtg cgcgctgccg tggatcgagt gcgcggtgcc
     1141 gacggactgc acgaaggcat cgacgcggcc cccgctctgc tgccagagct cctcgccgag
     1201 ggcgtgatag ccggcctcgc cgtcacggtt gttgagctgg tcacagtacc agtgcccggg
     1261 ccggcggctg atctcgtcgg ccttggcgat cagtgccctg atgagctgct cggtgatcgc
     1321 accgcccacc gcctcgacgt cggtgatctc ggcgccgtag gcccgcatgg tgtggcgctt
     1381 gtcgggactg aaggcgtcgg agaacacgaa gtgcgccttg aagccgagcg ccgcgcacac
     1441 gagcgctagc gagatgccgg tcgtgcccgc ggtgtactcg accaccgtgt cgcggggccg
     1501 caggcgcccg tccgcggcgg cgcgctcgat catggcgcgc gccacgcggt ccttcatgct
     1561 gccggtcggg ttggcggact cgagcttgac gacgaggcgc gcggctcctg cgcccgggac
     1621 gcgctgcagc tcgatgagcg gcgtgttgcc gatcgccgcc agtactccgc tcgagggcat
     1681 ggcgctctcc tgcaggccgc gctcagcgct gcggcagccg ctcgaagccg tgacggccga
     1741 tgagggccga gccgagccat ccggtcagcc agacgatgat ccaggtgagg agcgcggccc
     1801 gaaagccccc gtagaggtga aagcccggga ggatccaggc gacgagcgcc accatggcgg
     1861 cgttcagcac gaggaggaag aggccgagcg tcacgatggt gagcgggagc gtgaggatga
     1921 aggcgaccgg ccgcacgacc gcattcacga ggccgagcag gagcccggcg agcagcagcg
     1981 tcggcacatc gtcgatgcgc accccgtgta cccagcggct cgcgagccac agcccgaagg
     2041 cgctgagcag cgcgcgcagc aggaaaccgg tcacccggcc gctcccgccc cgcgttgccc
     2101 ctcgaccagc accttgagcc ggttgagggc cgccagccac gaggtccgca gccgccagta
     2161 ctgggtatcc cattcgggct cgcccggcag ccccgagccg aggagccgca cgaccgtgcc
     2221 ccccggcacg gcctcaatga gcaggtcgtc cgtgaccacc gactcggtgc gcggccaggc
     2281 agggttcggc agataaatca gccgcacccg gcggcccttc tcgaacacgt cgatgtgcgc
     2341 ctcgagctcc gtcagccggt caacgcgggc gcggaacaac ccgccctggc gcggttgcag
     2401 ctcagcctcg ggcgagcacc agcgggcgag gctctcg
//
LOCUS       DATLKL010000357         2405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_173307, whole genome shotgun sequence.
ACCESSION   DATLKL010000357 DATLKL010000000
VERSION     DATLKL010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173307"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VM713_04645"
     CDS             <1..800
                     /locus_tag="VM713_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HVH83589.1"
                     /translation="KTPRSCPATYIGLWDEVRRIYAGTTEARIRGYTASRFSFNTAGG
                     RCEACEGQGVKTIEMSFLPDVKVMCDVCGGRRFNSETLSVLWRGKSIGDVLAMDVDDA
                     VGFFAAHARVHRALKLLQDVGLGYLTLGQPSPTLSGGEAQRIKLVAELSKVRDDELPG
                     MAAPSRQTLYVLDEPTVGLHMADVEKLIHVLHRLVAAGNTAVVVEHNLDVMAEADWII
                     DLGPEAGAGGGRIVAQGPPAEIARRRKRSHTGEVLAQFLRERTLPAQ"
     gene            815..1117
                     /locus_tag="VM713_04650"
     CDS             815..1117
                     /locus_tag="VM713_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83590.1"
                     /translation="MAHDLVCWKCGAALAELSLPLRRLDECPKCHAELHVCRMCVDYD
                     TRVAKHCREPTADEVNDKTHANFCDHFKPRAAAYTPPNTAEVDKARAELERLFGKR"
     gene            complement(1122..2057)
                     /locus_tag="VM713_04655"
     CDS             complement(1122..2057)
                     /locus_tag="VM713_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83591.1"
                     /translation="MKPVFQDSNVREVLAALGETSTTGETRLMRLPPPPRNAPRAEGT
                     LSDTAVLRVLEERRTTEAEKTDDRQARNIPLLKPDDTGTRLALKEAVVNNAPVWFAVQ
                     LQWSVQPVELEKVPPLAIFSAYTLYTVEGSREGRHWYGLRLGFFSDAISAKQVAYYVR
                     SEFGSVAVVPVSPKERARATADEQAAAAVAAPERAPRADSGEFKLIGVEEEMRQAAAR
                     QVPARPAPKAPAPRPAEAGKPRSRLGRVGARERRGGPTLEETLEILGASELTMDTDRG
                     EGLNDTGVRHLRVEMHKNSPFSRLLERLSERVKKL"
BASE COUNT          332 a          828 c          884 g          361 t
ORIGIN      
        1 gcaagacccc gcgctcgtgt ccggccacct acatcggcct gtgggacgaa gtgcggcgta
       61 tctatgccgg caccaccgag gcgcgcatcc gcggctacac cgccagccgc ttctccttca
      121 acaccgccgg cggacgctgc gaggcgtgcg agggccaggg agtgaagacc atcgagatga
      181 gcttcctccc ggacgtcaag gtcatgtgcg acgtatgcgg cggccgccgc ttcaattcag
      241 agaccttgag cgtgctgtgg cgcggcaaga gcatcggcga cgtgctggcg atggacgtcg
      301 acgatgcggt cggcttcttc gcggcccacg cgcgcgtgca ccgtgcgctg aaactcctgc
      361 aggacgtagg cctcggctat ctcacgctcg ggcagccgag cccgacgctc tcgggaggcg
      421 aggcgcagcg catcaagctg gtcgcggagc tctccaaggt acgcgacgat gagctgccgg
      481 gcatggcggc cccgtcccgc cagactctct acgtgctcga tgagccgacg gtggggctgc
      541 acatggccga cgtcgagaag ctcatccacg tgctgcaccg cctggtcgcc gccggcaaca
      601 ccgcggtcgt ggtcgagcac aatctcgatg tcatggctga agccgactgg atcatcgact
      661 tgggccccga ggccggcgcc ggcggcggac ggatcgtcgc gcagggtccg ccggcggaga
      721 tcgcgcgccg gcgcaagcgc tcgcacaccg gggaggtgct ggcgcagttc ctgcgcgagc
      781 gcacgctacc ggcgcagtag tacgctaggc gtgcatggct catgatctcg tctgttggaa
      841 gtgcggcgct gccctcgccg agctcagcct gccgctgcgg cggctggacg agtgcccgaa
      901 gtgccacgcg gagctgcacg tgtgccgcat gtgcgtcgat tacgacacgc gtgtcgccaa
      961 gcactgccgc gagcccacgg cggatgaggt gaacgacaaa acgcacgcca acttctgcga
     1021 tcacttcaaa ccgcgcgccg cggcctacac cccgcccaat acggcggaag tcgacaaggc
     1081 gcgcgcggag ctcgagcgcc tgttcggtaa gcgctgagcg ctcagagttt cttgacgcgc
     1141 tcgctcaggc gctcgagcag ccgcgagaac ggcgagttct tgtgcatctc gacgcgcagg
     1201 tggcgcacgc cggtatcgtt gagaccctcg ccgcgatcgg tgtccatggt gagctcgctc
     1261 gctccgagta tctcgagcgt ctcttcgagg gtgggtccgc cgcggcgctc gcgtgcgccg
     1321 accctgccta ggcggctgcg gggcttgccg gcttcggcag gtctgggagc cggtgccttg
     1381 ggggccggcc gtgcggggac ctggcgggcg gccgcttgcc gcatctcctc ctcgacgccg
     1441 atgagcttga actcccctga gtcggcccgc ggcgcgcgct cgggcgcagc gaccgcggca
     1501 gccgcctgct cgtcggcggt ggcgcgggcg cgctccttcg ggctcaccgg cacgaccgcg
     1561 acggagccga actccgagcg cacgtagtag gcgacctgct tggcggagat cgcgtcgctg
     1621 aagaatccga ggcgcagccc gtaccagtgg cggccctcgc ggctgccttc gaccgtatag
     1681 agggtgtagg cgctgaagat cgcgagcggt ggcaccttct cgagctcgac cggctgcacc
     1741 gaccactgca gctgcaccgc gaaccacacc ggggcgttat tgaccacggc ctccttgagc
     1801 gccaggcggg tgccggtgtc gtcgggcttc agcagcggga tgtttctcgc ctgccggtcg
     1861 tcggtcttct cggcttcggt ggtgcggcgc tcttcgagga cccgcagaac cgccgtgtcg
     1921 ctgagcgtcc cctcggcgcg cggcgcattg cgcggcggcg gcggcaggcg catcaggcgc
     1981 gtttcgcccg tcgtgctggt ctcgccgaga gcggcgagaa cctcacgtac gttggaatcc
     2041 tggaatacgg gcttcaccgg ggcgcggcct gcagcgtctg cgcgcggcct ggccgccgcg
     2101 gccggcggct gcgggcgcgc cggggttcgg ggtgcggctc ttgccgtcgg aatgcttacc
     2161 ggcgccggac gcgcggcggc aacccgcgcg gccacgggcg cctggcgcac gcccgcaggg
     2221 ggcgcggccg ctctcacggt gggaggaatc tggggtgccc ggcgcgcaac cggcgcgctg
     2281 atcggagcgg gtcggggagt cccacggagc gcagcctgcg aggcggcttg aaccaccggc
     2341 acccgcagcc cagggggcgg accggcccgc ggcacgggag gcctgggggc ggcgacgcgg
     2401 gtcgc
//
LOCUS       DATLKL010000358         2027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_83085, whole genome shotgun sequence.
ACCESSION   DATLKL010000358 DATLKL010000000
VERSION     DATLKL010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83085"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..727)
                     /gene="pdsR"
                     /locus_tag="VM713_04660"
     CDS             complement(38..727)
                     /gene="pdsR"
                     /locus_tag="VM713_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HVH83592.1"
                     /translation="MKRLIAIVEDEAAIRDNYAAAFAREGYAVRTYANRAQAMSAFAT
                     RLPDLAVIDISLEDEPEGGFELCRQLRALSPVLPIIFLTARDSELDAVSGLRLGADDF
                     LTKDLSLAHLSARVNALFRRVDALRKPEQAGDILRRGPLTLDTERMQAQWDGKVVLLS
                     LTEFWIVHALARHPGHVRNRQQLMDAANVVLDDNTITSHVKRIRRKFQGVDPQFDAVQ
                     TVYGMGYRWVE"
     gene            884..1006
                     /locus_tag="VM713_04665"
     CDS             884..1006
                     /locus_tag="VM713_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83593.1"
                     /translation="MSRRYQTPADTAAPSGPRWGTLADWLALLMLLAGSVVWPR"
     gene            1222..>2027
                     /locus_tag="VM713_04670"
     CDS             1222..>2027
                     /locus_tag="VM713_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HVH83594.1"
                     /translation="MFTQVLDPLDNLTLTCLVALIPVVSLLVMLAVFRMPAWLATLIG
                     SLITFVLALWVWKMPMEQGSHAYLYGAATGVWNVDWITFWGMMLFNTLSVSGLFENFR
                     RWLISKGSLDVRVQTILFAWAFGALLEGLVGFGYPWAIVAPIPISLGISDINAIRVAA
                     IANNAPVSYGALGAPIIALAAVTNYPLLALSGSIGTIVAILALLPPWVLLYLVSGKEG
                     MKGGWPLAIVGSLGYIAGQYPTAVYWGPYLPDVVGSIVCFAALLILLKVW"
BASE COUNT          313 a          695 c          671 g          348 t
ORIGIN      
        1 cagcagcagc ttcaggcgca gcgacatcgt cagggggcta ctcgacccag cggtagccca
       61 tgccgtagac ggtctgtacg gcgtcgaact gcggatcgac gccctggaac ttgcgccgaa
      121 tgcgcttcac gtgcgaggtg atggtgttgt cgtcgagtac cacgttggcg gcgtccatca
      181 gctgctggcg gttgcgcacg tggccgggat ggcgggcgag ggcgtgcacg atccagaact
      241 cggtgagcga cagcagcacc accttgccgt cccactgtgc ctgcatgcgc tcggtgtcga
      301 gcgtgagcgg gccgcgccgc agtatatcgc cggcctgctc gggtttccgc aacgcatcga
      361 cgcggcgaaa cagcgcattc acgcgcgccg acaggtgcgc gaggctcagg tccttggtga
      421 ggaaatcgtc ggcaccgagg cgcagccccg agacggcgtc aagctcgctg tcgcgcgcgg
      481 tgagaaagat gattggcagc accggtgaga gcgcgcgcag ctgccggcac agctcgaagc
      541 ccccctcggg ctcgtcctcg agcgagatgt cgatgacggc gagatccgga aggcgcgtcg
      601 cgaaagcgct catggcctgc gcgcggttcg cataggtgcg caccgcataa ccttcgcgcg
      661 cgaacgcggc ggcgtagttg tcgcggatgg ccgcttcatc ctcgacgatg gcgatcaggc
      721 gcttcatgac agcgacaggc taccgcctgc gcgcggccgg tgaaagcggt gtgcgtcacc
      781 gaactggcac ggcgcgcgcg ccgccacatc tcgccacgat tcgccgcccg cgtgcccgat
      841 tggtgccgtg ggccggcaat ccccggagcg cgaaatactc gacatgagcc gccgctacca
      901 gacacccgcc gacaccgccg cgccttccgg accgcgctgg ggcacgctcg ccgactggct
      961 cgcgctcctc atgctgctcg ccggcagcgt ggtgtggccg cgctgaggcg cggccagccg
     1021 ctcgagccca gcccgcggcg cgtaaccgcc gcgcgcccgt gccaacgttg agccgctccc
     1081 ccggtacgtg ctaggttgtc cggcctcaag cgccggacgc gctggtgttg gatccaccag
     1141 gagcagtcgc aaccagaaga agcaaagcgc cgcgaccccc gagacacgct catcgcgccg
     1201 ctcaagttga ggggataaac catgttcacc caagtactcg acccgctcga caacctgacg
     1261 ctcacctgcc tcgtggcgct gattccagtc gtgtcgctgc tcgtcatgct ggccgtgttc
     1321 cgcatgccgg cgtggctcgc gacgctcatc gggtcgctga tcacctttgt gctggcgctg
     1381 tgggtgtgga agatgcccat ggaacagggc tcccacgcct atctctacgg cgccgcgacc
     1441 ggcgtgtgga acgtcgactg gatcaccttc tggggaatga tgctgttcaa cacgctctcg
     1501 gtgagcgggc tgttcgagaa tttccgccgc tggctcatca gcaagggcag cctcgacgtg
     1561 cgcgtgcaga cgatcctgtt cgcctgggca ttcggcgcgc tgctcgaagg gctggtcggc
     1621 ttcggctatc cgtgggcgat cgtggcgccg atcccgatct ccctcggaat ctccgacatc
     1681 aacgcgatcc gcgtcgcggc aatcgccaac aacgcgcccg tctcctacgg cgcgctcggc
     1741 gcgccgatca tcgcgctcgc cgccgttacc aactatccgc tgctggcatt atcgggctcg
     1801 atcggcacca tcgtcgcgat actggcactg ctgccgccgt gggtgctgct gtacctggtc
     1861 tccggcaagg aaggcatgaa gggcggctgg ccgctcgcga tcgtcggctc gctcggctac
     1921 atcgccgggc agtatccgac cgcggtttac tgggggccat acctgccgga cgtggtcggc
     1981 tcgatcgtct gcttcgccgc gctcctcatc ctgctcaagg tgtggca
//
LOCUS       DATLKL010000359         3002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_150975, whole genome shotgun sequence.
ACCESSION   DATLKL010000359 DATLKL010000000
VERSION     DATLKL010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150975"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VM713_04675"
     CDS             complement(<1..1268)
                     /locus_tag="VM713_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HVH83595.1"
                     /translation="MNQTGKRKARILVVDDDPGLLRLLTIRLRAESYDVEAVESAAQA
                     LAAASRFRPDLVITDLRMDQMDGIGLLKELQNRWPGLKVIILTAHGTIPDAVQATQMG
                     AFGFLTKPVDKQELLDQVQKALRISGFGASDEDWRAEIVTRSAVMEEKLAQAHMVAGT
                     DARVLITGESGTGKELLARAIHRASPRRNKPFVAINCSAMAENLLEAELFGQEKGPVT
                     AATRAHQGLFVAADGGTLLLDEIGDMPMRLQVKLLRVLQENAIRPASGGDAIPVNVRV
                     ISATHRDLQQLMVSGQFREDLYYRLNVVHIEIPPLSRRREDIPLLVAHFLAQIARESG
                     VRKIYAPEAVELLATADWAGNIRQLQNVVRQNVALSQTPIIPVELVQQSLGGTPGRLP
                     SFDEARDEFTRSYLSQILQITGGNVSQAARL"
     gene            complement(1286..1933)
                     /locus_tag="VM713_04680"
     CDS             complement(1286..1933)
                     /locus_tag="VM713_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83596.1"
                     /translation="MRAPPEHCRTRLLAALVLLALAGCNGKLGLLSRDRAAPAPRPAQ
                     TASTSSLLLSDYLLLLQRLVQGSASEQAEIVARAQNEYETAPTPSRELKLALVLSTPG
                     HPATDQPRAQGLLRELMAHPETLAPAERALAFLQLSQIDDHLTLVAENRRLQTDAVKE
                     DQARTASANRRLQAELDENARLRKELDEARAKLDAIANIERSLNERKPGTTTPHP"
     gene            complement(1917..>3002)
                     /locus_tag="VM713_04685"
     CDS             complement(1917..>3002)
                     /locus_tag="VM713_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVH83597.1"
                     /translation="TTSDLSARFTQLSVLVEQVAQQSNAQVDTEVAALEAQTLRARQQ
                     LLWESALLLPLALLAVLVLTVGVGRPLRQLDRAISELGQGNLTNTIAVAGPHDLERLG
                     RQLEWLRQRLLDLAHERNRFLRHMSHELKTPLANIREGTELLMDGAVGALDSSQREVT
                     AILRENGIKLQRMIENLLSFSAWQTSRVGLEATEFRLRPLVKQVLENQQLTLLSQRVR
                     LEVRVEDITLVADRGKIRLILENLVSNAVKYSPKGGTIHVRARAAGARAILDVADSGP
                     GIPPEDRAHIFDAFYTGRAARSTAVKGTGIGLSVVLEFVAAHNGTVEIVDGEFTGAHF
                     RITMPISATTGDQPVTVAKAKAHARAA"
BASE COUNT          429 a         1003 c         1065 g          505 t
ORIGIN      
        1 aggcgcgcgg cctgactcac gttgccgccg gtgatctgca ggatctgcga gaggtagctg
       61 cgcgtgaact catcgcgcgc ctcgtcgaag gagggcagcc gcccgggcgt gccgccgagc
      121 gactgctgca ccagctccac cgggatgatg ggggtctgcg acagcgcgac gttctgccgc
      181 accacgttct gcagctggcg gatgttgccg gcccagtcgg cggtggcgag cagctcgacc
      241 gcctcgggtg cgtagatctt gcgcaccccg ctctcgcggg cgatctgcgc gaggaaatgc
      301 gcgaccagga gcggaatgtc ctcgcggcgg cggctcaagg gcggaatctc gatgtgcacg
      361 acgttcagcc ggtagtagag atcctcgcga aactgtccgc tcaccatcag ctgctgcagg
      421 tcgcgatggg tggcggagat cacgcgcaca ttgaccggga tcgcatcgcc gccgctcgcg
      481 gggcggatgg cgttctcctg cagcacgcgc agcagcttca cctgcagccg catcggcatg
      541 tcgccgatct catcgagcag gagcgtgccg ccgtctgccg ccacgaacag gccctggtgc
      601 gcgcgcgtcg ccgcggtgac cgggcccttc tcctggccga acagctccgc ttccagcagg
      661 ttctccgcca tcgcgctgca gttgatggcg acgaacggct tgttgcggcg gggactcgcg
      721 cgatggatgg cgcgtgccag taattccttg ccggtgccgc tctcgccggt gatgagcacg
      781 cgcgcatcgg ttcccgcaac catgtgcgcc tgagcgagct tttcctccat cacggcgctg
      841 cgcgtgacga tctccgcgcg ccagtcttcg tccgaggcgc caaagcccga gatgcgcagc
      901 gccttctgca cctggtcaag cagctcctgc ttgtcgacgg gcttcgtgag gaagccgaac
      961 gcgcccatct gcgtcgcctg cacggcatcg gggatggtgc cgtgcgcggt gaggatgatg
     1021 accttgagtc ccggccagcg gttctgcagc tccttgagca gcccgatgcc gtccatctgg
     1081 tccatgcgca ggtcggtgat caccaggtcc gggcggaagc ggctggcggc cgcgagcgcc
     1141 tgggcggcgc tctcgaccgc ctcgacgtcg tagctctccg cgcgcaggcg gatcgtgagg
     1201 aggcgcagca gccccgggtc gtcgtcgacc acgagaatgc gtgctttgcg ttttccggtc
     1261 tgattcaagt gcggctcctg tcgcatcacg ggtgcggggt ggtcgtgcca ggcttgcgct
     1321 cattcagcga gcgctcgatg ttggcgatcg catcgagctt ggcgcgcgcc tcgtccagct
     1381 ccttgcgcag gcgcgcgttc tcatcgagct ccgcctgcag ccggcggttg gcgctcgccg
     1441 tgcgcgcctg gtcctctttc accgcatcgg tttggagcct gcgattttcc gcgaccagcg
     1501 tcaaatggtc gtcaatttgc gaaagctgca gaaacgcgag cgcgcgctcg gccggcgcca
     1561 gagtctcggg atgcgccatc agctcgcgca gcaggccctg ggcgcgcggc tggtcggtcg
     1621 ccgggtgacc cggagtcgac agcaccaggg cgagcttgag ctcgcggctc ggcgtcggcg
     1681 ctgtctcgta ctcgttctgg gcgcgcgcga cgatttccgc ctgctcggag gcgctgccct
     1741 gcaccaggcg ctgcagcagc agcaggtaat ccgacagcag gagcgaggag gtcgaggcgg
     1801 tctgcgccgg ccgcggggcg ggcgcggccc ggtcgcggga caggaggccc aacttgccgt
     1861 tgcagcccgc gagcgccagc agcacgaggg ctgccagcag ccgagtgcgg cagtgctcag
     1921 gcggcgcgcg catgtgcttt ggccttcgca accgtaaccg gctgatcgcc ggtggtggcg
     1981 ctgatcggca tggtgatgcg gaaatgcgcc ccggtgaact caccgtcgac gatctccacc
     2041 gtgccgttgt gtgccgcgac gaactcgagc accaccgaca atccgatgcc ggtgcccttc
     2101 accgccgtgc tgcgcgccgc acggcccgtg tagaaggcgt cgaaaatatg cgcgcggtcc
     2161 tcgggcggaa tgccggggcc gctgtcggcc acgtcgagaa tggcgcgggc gcccgcggcg
     2221 cgtgcccgca cgtggatggt gccgcccttg ggcgagtact tgacggcgtt cgataccagg
     2281 ttctcgagga tcaggcgaat cttgccgcgg tcggccacca gcgtgatgtc ctcgacgcgc
     2341 acctcgagcc gcacgcgctg cgagaggagc gtcaactgct ggttctccag tacctgcttc
     2401 acgagcggac ggagccggaa ctcggtggcc tcgaggccga cgcgcgaagt ctgccaggcg
     2461 ctgaacgaca ggagattctc gatcatgcgc tgcagcttga tgccgttctc gcgcaggatc
     2521 gccgtgactt cgcgctggct cgagtcgagc gcgccgaccg cgccgtccat gaggagctcc
     2581 gtcccttcgc gaatgttggc gagcggggtc ttcagctcgt gcgacatgtg gcggagaaag
     2641 cggttgcgct catgcgccag gtcgagcagc cgctgacgca gccattcgag ctgccggccg
     2701 agccgctcga gatcgtgggg cccggcgacg gcgatggtgt tggtcagatt cccctggccg
     2761 agctcgctga ttgcgcggtc gagctggcgc agcggccggc cgacgccgac ggtgagcacg
     2821 agcaccgcga gcagcgcgag cggcaggagc agtgccgact cccacagcag ctgctggcgc
     2881 gcgcgcagcg tctgcgcttc gagcgccgcg acctcggtgt cgacctgggc gttgctctgc
     2941 tgggccacct gctcgaccag caccgacagc tgagtgaagc gcgccgacag atcggacgtg
     3001 gt
//
LOCUS       DATLKL010000360         1888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_94490, whole genome shotgun sequence.
ACCESSION   DATLKL010000360 DATLKL010000000
VERSION     DATLKL010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94490"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VM713_04690"
     CDS             complement(<1..610)
                     /locus_tag="VM713_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH83598.1"
                     /translation="MTSNVRILLVEDDAMLGEAVRDGLRQLGYVVDWVQDGGPARAAL
                     ATTSFGAVVLDLGLPTGGGVSVLRWLRHSGRNTPVVIVTARDRVTDRIAGLDAGADDY
                     LIKPFDIDELGARLRAVTRRTEGAVDSVLTVGDVVLDLRQRIVTCRGEQAALTAREYA
                     VVELLMRKSGCLVTRAEIEEELYGFDDDIASNAIEVHIHNLRR"
     gene            673..1326
                     /locus_tag="VM713_04695"
     CDS             673..1326
                     /locus_tag="VM713_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVH83599.1"
                     /translation="MFSIFTAARARASLSALAILLLAAAPPCFAGGGPFGIDHEWSYD
                     NSGIWARSNQQVLEYGLIAFEVGGAVWEGGDTRFGRTLWQSIDSSAASGIVALALKHA
                     FSRVRPIDSGGDPNLWFKGHGNQSFPSGEVTEVSAIITPIVLEYRHDDPWVYALELLP
                     VYDGIARMKVQAHWQSDVIAGFALGTGFGWLMHNHEGTPFVLSVLPHGFYVGLKKSF"
     gene            1371..>1888
                     /locus_tag="VM713_04700"
     CDS             1371..>1888
                     /locus_tag="VM713_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HVH83600.1"
                     /translation="MLLDSTPPHAPASQVTEREGGIVAVSFRAMASPCEVLLETTDRL
                     LAAELGQAAAREAWRIELKYSRYRPESIVSVINRSDGHAVVVDDETAALLDYAARCHA
                     LSLGRFDITSGVLRHCWKFDGSDRLPDPRVVEKLLPLIGFDKLRWQPPRITLPPGMEI
                     DFGGFGKEYAVDR"
BASE COUNT          297 a          633 c          626 g          332 t
ORIGIN      
        1 tgcggcgcag gttgtggatg tggacctcga tggcgttgct ggcgatgtcg tcatcgaagc
       61 catacagctc ctcctcgatt tcggcgcgcg taaccaggca gcccgacttg cgcatcagca
      121 gctccaccac cgcgtactca cgcgcggtga gcgcggcctg ctcgccccgg caggtgacga
      181 tgcgctggcg cagatcgagc acgacatcgc ccaccgtgag caccgagtcc acggcgccct
      241 cggtgcggcg cgtgacggcg cgcaggcgcg cgccgagctc gtcgatgtca aagggtttga
      301 tgagatagtc gtccgcaccg gcgtcgaggc cggcgatgcg gtcggtgacg cgatcgcgcg
      361 ccgtgacgat gacgaccgga gtgttgcggc cgctgtgccg cagccaccgc agtacgctca
      421 cgccgccgcc cgtcggcagg ccgagatcga gcaccaccgc gccgaagctc gtggtcgcga
      481 gcgccgcgcg cgccgggccc ccgtcctgta cccaatcgac gacatagccg agctgacgca
      541 gtccgtcgcg gaccgcctca ccgagcatcg catcatcttc gaccagcagg atccgcacgt
      601 tcgaggtcat cccgggctga gcgcccattt gatcacgccg cgcttaagtc tgcggctcct
      661 attctcgagt caatgttctc catcttcacc gcagcgcgag cgcgcgcgtc cttgagcgcg
      721 ctggccattc tactgctcgc ggcggcgccg ccgtgctttg ccggcggcgg ccccttcggc
      781 atcgatcacg aatggagcta cgacaacagc ggcatctggg cgcgctcgaa ccagcaagtg
      841 ctcgagtacg gcctgatcgc cttcgaagtg ggcggagcgg tgtgggaagg tggggacacg
      901 cgctttggcc gcacgctttg gcaatcgatc gattcttcgg cggcctcggg catcgtcgct
      961 ctggcactca agcacgcatt ctcgcgcgtg cgcccgatcg acagcggcgg ggatcccaac
     1021 ctgtggttca agggtcatgg gaaccagagc tttccgagcg gcgaggtcac ggaggtgagc
     1081 gcgatcatca ctccgatcgt gctcgagtac cgtcacgatg acccctgggt ctacgcgctc
     1141 gagctgctgc cggtctacga cgggatcgcg cgcatgaagg tacaggccca ctggcagtcg
     1201 gatgtcatcg cgggctttgc gcttggtacc ggcttcgggt ggctgatgca caatcacgag
     1261 gggaccccgt tcgtgctgag cgttctgccg cacgggttct atgtgggact caagaaaagc
     1321 ttctaggacg gcgctcgacc tcggggcgct caaaggccgc gtcgtctagc gtgttgctcg
     1381 attccacacc gccgcacgcg ccagcgtccc aggtgaccga gcgcgagggc ggcatcgtcg
     1441 cggtgtcatt ccgggcgatg gcaagcccgt gcgaggtgtt gctcgagacc accgacaggc
     1501 tgctggcggc ggagctcggg caggccgcgg cgcgcgaggc gtggcgcatc gagctcaagt
     1561 acagccgtta ccgtcccgag agcatcgtca gcgtcatcaa ccgcagcgac gggcacgccg
     1621 tggtcgtaga cgatgagacc gcggcgctcc tcgactacgc cgcacgctgt cacgctctct
     1681 cgcttgggcg tttcgacatc acttcgggcg tactgcgcca ctgctggaag ttcgatggct
     1741 ccgatcgcct tcccgacccg cgcgtcgtcg agaaactcct gccgctgatc ggtttcgaca
     1801 agcttcgctg gcagccgcct cgcatcactc tgcctccggg catggagatc gacttcggcg
     1861 gctttggcaa ggagtatgcc gtggaccg
//
LOCUS       DATLKL010000361         2184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_150984, whole genome shotgun sequence.
ACCESSION   DATLKL010000361 DATLKL010000000
VERSION     DATLKL010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150984"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            452..1021
                     /locus_tag="VM713_04705"
     CDS             452..1021
                     /locus_tag="VM713_04705"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HVH83601.1"
                     /translation="MIGRGLLTTLCAVALTAAVARAHADALDGVLVVRSAYVNIDHGV
                     FLLHARVDYPEGPAIRKALRDGVTLDFDLDVRIERERRLWFNANIVELTLHRELGYHA
                     VSDRYVVRDVRSGDQDSFATLDEALDYLGKVNGWPILVEPQLDGGNYSISVRAGVRRG
                     QLPASLRALLFWTDDWARQSEWYTWSLPV"
     gene            1054..>2184
                     /locus_tag="VM713_04710"
     CDS             1054..>2184
                     /locus_tag="VM713_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HVH83602.1"
                     /translation="MAALGAFWGVTGVGAFLLLAKSVQNSAEFERLQWWIVVLNVCAV
                     LVLTVLVVQKLSRLVRDYRAHVPGSRLTARTVGIFGALVIAPLLIVYLSSLEFLNRGI
                     DSWFTVEVKQGLNDAVVLSRAALDLRTREYSQRTQTLARELERLPPAALQARLDAERR
                     AGEAREIALFGAQGRIVAASLENPLEALPTPPPPDLVRQVAQHLPYVALEPQAGGSYL
                     IRTGAALANTAASHDARFVVGVYPVPAQLAALSGAVQGTYSQYGNLAAMREPLKYSFS
                     LTLTLVLLLAMLAAIYGAIFSAQRLVRPVQDLIEGTRAVGKGDFATRLPLPSRDEMGF
                     LVHSFNDMTKRLRRARAEAQHSQEAVERERERLAIILARLSTG"
BASE COUNT          302 a          737 c          790 g          355 t
ORIGIN      
        1 gttctggtgg acgcgccgtg ctcatccacc ggcgtgatcc gccgtcatcc ggatatcaag
       61 ctgttgcggc gcgccagcga cctgccggcg ctcgtggcgg cgcagctcgc gatcctcgcg
      121 ggggcggccc gcatgctggc cgtgggcggg cgcctggtgt attgcacttg ctcggtgctg
      181 cccgaggaga acgagggggt ggtgcagcgc ttcctcgagg gggcgcccca catgaccccc
      241 ggcagcatgc cgcgcgccgc cgctctcgct cccggcgcgc gcgaccgggc ggtgggggta
      301 cagctgcttc cgggggcaga gacggggacc gacggcttct attatgcttg tctcgaaaag
      361 acaacggccg gaacccgagc cgtagcccaa agtccacagt ggagagccag tcgttgcatc
      421 catgctacgc ggcggtccag caagggatgg tttgatcggg cgcggcctgc tcacgaccct
      481 ttgtgcggtg gcgctgaccg cggcggtcgc gcgggcgcac gcggatgccc tcgacggagt
      541 gctcgtcgtg cggtccgcgt acgtgaacat cgaccatggg gtgttcctgc tgcacgcccg
      601 ggtggattac ccggaagggc ccgccatccg taaggcgctg cgggatgggg tcaccctgga
      661 tttcgatctg gatgtgcgca tcgagcggga gcggcggctc tggttcaacg ccaacatcgt
      721 cgagctgaca ttgcaccgcg agctcggcta tcacgcggtg agcgaccgct acgtggtccg
      781 tgacgtgcgc agcggcgacc aggacagctt cgcaactctc gacgaggcgc tcgactacct
      841 cggcaaggtg aacggctggc cgatcctggt ggagccgcag ctcgacggcg gcaattacag
      901 catcagcgtg cgggccgggg tgcgccgtgg gcagctgccg gcgtcgctcc gggcgctgct
      961 gttctggacc gacgactggg caaggcagag cgagtggtac acatggtcgc tgcccgtgta
     1021 agtcaccgca gccaggcccg caggcgggcg accgtggccg cactcggcgc cttctggggc
     1081 gtgaccggcg tcggcgcctt tctgttgctc gcgaagagcg tacagaactc ggcggaattc
     1141 gagcgtctgc agtggtggat cgtggtcctc aacgtgtgcg cggtgctggt gctcaccgtg
     1201 ctggtggtgc agaagctctc gcggctggtg cgcgattatc gcgcgcatgt gccgggttcg
     1261 cggctcacgg cgcgcaccgt cggcatcttc ggtgcgctgg tgatagcgcc gctcctgatc
     1321 gtgtacctgt cgtcgctcga gttcctgaac cgcggcatcg acagctggtt cacggtggaa
     1381 gtgaagcagg ggctcaacga tgccgtggtg ctgtcgcgcg ccgcgctcga cctgcgcacg
     1441 cgcgagtact cgcagcgcac ccagactctg gcgcgtgagc tcgagcggct gccgcccgcg
     1501 gcgctgcagg cgcgcctcga tgccgagcgg cgtgcgggcg aggcgcgcga gatcgcgctc
     1561 ttcggcgcgc agggacgcat cgtcgcggcg agcctcgaga acccgctcga agcgctgccg
     1621 acgccgccgc ccccggacct ggtgcggcag gtggcgcagc accttcccta cgttgccctc
     1681 gagccccagg ccggcggcag ttacctgatc cgcacaggag ctgcgctcgc caacaccgcc
     1741 gcgagccacg acgcgcgctt cgtcgtcggc gtctacccgg tgccggcgca gctcgcggcg
     1801 ctctcggggg cggtgcaggg cacgtactcg cagtacggca atctcgccgc aatgcgcgag
     1861 ccgctcaagt acagcttcag cctcaccctc acactggtgc tgctgctcgc gatgctggcc
     1921 gccatctacg gcgcgatctt ctcggcgcag cgcctggtgc gcccggtgca ggacctcatc
     1981 gagggcacac gcgcggtcgg caagggcgat ttcgccacgc ggctgccgct gccgtcgcgc
     2041 gatgagatgg gctttctggt gcactccttc aacgacatga ccaagcgcct gcggcgcgcg
     2101 cgcgccgagg cgcagcacag ccaggaggcg gtggagcgcg agcgcgagcg cctcgcgatc
     2161 atcctcgcgc gcctgtcgac cggg
//
LOCUS       DATLKL010000362         1737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_105724, whole genome shotgun sequence.
ACCESSION   DATLKL010000362 DATLKL010000000
VERSION     DATLKL010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105724"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VM713_04715"
     CDS             complement(<1..353)
                     /locus_tag="VM713_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83603.1"
                     /translation="MSPLWRDEVGVYLSPHRLCLVRMKRGVRPVMVAEHELRFEGVRG
                     DSWSAPLEALAGLLAEPAWSGAQTRVVVADHWVRYAVVPWAAALSSADERVAHGRQVL
                     ASLYGEAVAGWDVRIS"
     gene            complement(334..>1737)
                     /locus_tag="VM713_04720"
     CDS             complement(334..>1737)
                     /locus_tag="VM713_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HVH83604.1"
                     /translation="AIFTDPFDEVLRGWLELRITAPVEWALAAHEEVSNLIARSAEAL
                     RAIDTMVPESEEHVRGDEGVENLSYVKISEDTSPVVRLVHSTVYDALRAGASDIHLES
                     RASGLAVRYRLDGVLVDITTVRGADLTKQIISRIKVMSELDIAERRVPQDGRFKIAIN
                     GRPIDFRVSIIPSIFGEDAVLRILDKQALTKMAGLRLDVLGFEAGIVEQLRRLAALPY
                     GMLLVTGPTGSGKTTTLYAAISETQTGRDKIITIEDPVEYQLPGVLQIPVNEKKGLTF
                     ARGLRSILRHDPDKIMVGEIRDPETAQIAIQSALTGHLVFTTVHANNVFDVVSRFTHM
                     GVDSHGFVSALSAVMAQRLVRIVCEQCAEAYMPARELLEESHVPIVTAASYKFRKGRG
                     CGHCRGTGYHGRRAVGELMVLNDEIREAIVNRVPVRQLKDLAHRAGVRLIRSAALDLV
                     RCGQTTVEEVNRVTIMA"
BASE COUNT          275 a          604 c          584 g          274 t
ORIGIN      
        1 gagatgcgga cgtcccagcc cgcgacggcc tcgccataga gactcgcgag aacctgtcgg
       61 ccgtgcgcca cgcgctcgtc cgcggagctg agcgcggccg cccagggcac gaccgcgtag
      121 cgcacccaat ggtccgcgac cactacgcga gtctgtgcgc cgctccaggc gggctcggcg
      181 aggagtcccg cgagcgcctc gagcggtgcc gaccaggaat cgcccctgac cccttcgaaa
      241 cgcagctcgt gctcggcgac catgacgggc ctcacgccgc gcttcatgcg caccaggcac
      301 aggcggtgcg gcgagaggta gaccccgacc tcgtcacgcc ataatggtga cacggttcac
      361 ctcctcgacc gtcgtctgtc cgcagcgcac cagatcgagc gccgcgctgc gaatgaggcg
      421 cacaccggcc cgatgcgcca gatccttgag ctgccgcacc ggcacccggt tcacgatcgc
      481 ctcgcggatc tcatcgttca gcaccatgag ctcgcccacc gcccggcggc cgtggtagcc
      541 ggtgccgcgg caatggccgc agccgcgccc cttgcggaac ttgtaggagg cggcggtcac
      601 gatcggcacg tgcgactcct cgaggagctc gcgcgcaggc atgtaggcct cggcgcactg
      661 ctcgcacacg atgcgcacca ggcgctgggc catcacggcc gagagcgccg aaacaaagcc
      721 gtgggagtcg actcccatgt gggtgaagcg gctgaccacg tcgaacacgt tattggcgtg
      781 cacggtggtg aacaccagat gcccggtgag ggccgactgg atcgcaatct gcgccgtctc
      841 gggatcgcga atctcgccga ccatgatctt gtcggggtcg tggcgcagga tggagcgcag
      901 cccgcgcgcg aaggtcaggc ccttcttctc gttcaccggg atctgcagca cgccgggcag
      961 ctggtactcg accggatcct cgatggtgat gatcttgtcg cgcccggtct gggtctcgga
     1021 gatcgcggcg tagagcgtgg tggttttgcc gctgcccgtg ggtccggtga cgagcagcat
     1081 gccgtagggg agcgccgcga ggcgccgcag ctgctcgacg atgcccgcct cgaagccgag
     1141 cacatcgagc cgcagccccg ccatcttggt gagcgcctgc ttgtcgagga tgcgcagcac
     1201 cgcgtcctcg ccgaagatgc tcgggatgat cgacacgcgg aagtcgatcg ggcggccgtt
     1261 gatcgcgatc ttgaagcgtc cgtcctgcgg cacgcggcgc tcggcgatgt cgagctccga
     1321 catcaccttg atgcgcgaga tgatctgctt ggtgaggtcg gcgccgcgca cggtcgtgat
     1381 gtcgaccagc acgccgtcga gccgatagcg caccgcgagc ccgctggccc tcgactcgag
     1441 gtggatgtcg ctcgccccgg cgcgcagcgc gtcgtacacg gtggagtgca ccaggcgtac
     1501 caccggactc gtatcttcgc tgatcttgac gtaggagaga ttctcgaccc cctcgtcgcc
     1561 cctgacgtgc tcctcgctct cgggcaccat cgtatcgatg gcacgcagcg cctcggcgct
     1621 gcgcgcgatc aggttcgaga cttcctcgtg ggccgcgagc gcccattcga ccggcgccgt
     1681 gatgcgcagc tccagccagc cgcgcagcac ctcatcgaag ggatcggtga agatggc
//
LOCUS       DATLKL010000363         2332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_49249, whole genome shotgun sequence.
ACCESSION   DATLKL010000363 DATLKL010000000
VERSION     DATLKL010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49249"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VM713_04725"
     CDS             <1..1097
                     /locus_tag="VM713_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVH83605.1"
                     /translation="ALGKVVVGDPRLEQVRMGPVASLSQRREVSEQLAKLEQEAEVVA
                     GGTGKPQLLGADPERGAFIAPTLLYCRDAARSRAIHAVEAFGPVCTVVPYRDTGEAIA
                     LARRGGGSLAGSVFTADDRLAAQLVLALAPFHGRIVVVNRHCAKESTGHGSPLPHLVH
                     GGPGRAGGGEEMGGIRGVLHYLQRTAVQGTPDVITATSGRWTKGARELDPGTHPFRKP
                     FGSLAIGDTFHSGERQVTVEDIERFAALSGDRFYAHMGEAEAARNPLFGGRVAHGYFL
                     VAAAAGLFVDAPYGPVLANYGIDGLRFVKPVKPGDRIRVRLTCKEKSLRAGAGYGEVR
                     WDTAITNQNGEAVANYDVLTMVSEKAIPDA"
     gene            1169..>2332
                     /locus_tag="VM713_04730"
     CDS             1169..>2332
                     /locus_tag="VM713_04730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVH83606.1"
                     /translation="MSDAGHSTRADVVIIGAGPAGSTAAILLAARGYRVALLEKAHHP
                     RFHIGESLLPANRPLFEQLGIAERVRAIGMLKWGAEFVSPWHGRSEVFEFASARNPEL
                     PFSYQVRRSQFDEILIRRAAECGATVVEGCRAREVELERPDGGVRVVAEHEDGSKSAW
                     QARFLIDASGRDTFLANHLHVKHRNPRHNSAAIYAHFAGVERRAGKRAGDISIYWFEY
                     GWFWLIPLADGVTSVGAVVWPHYLKQRRGPMPEFLLATIARCAPLAERLKDATLASQV
                     EATGNYSYSASLSHGRNYLLVGDAFAFIDPVFSTGVWLAMHGGVAACEAVDTCLRIPA
                     RAGAALRAYDRTTRRGPRLLSWFIYRVTNPALRDLLMAPRNALRMRDTLLSVLA"
BASE COUNT          336 a          784 c          836 g          376 t
ORIGIN      
        1 ccgcgctcgg caaagtcgtg gtgggcgatc cgcgcctcga gcaggtgcgc atggggccgg
       61 tcgcctcgct cagccagcga cgcgaggtgt ccgaacagct cgcgaagctc gagcaggaag
      121 cggaagtggt cgcgggcggg acgggcaaac cgcagctgct cggcgccgac cccgagcggg
      181 gcgcgttcat tgcgccgacg ctgctctatt gccgggacgc cgcgcgctcg cgcgccatcc
      241 acgcggtcga ggctttcggc ccggtgtgca cggtggtgcc ataccgggac acgggcgagg
      301 cgatcgcgct cgcgcgccgc ggcggcggga gtctggcggg ctcggtgttc acggcggacg
      361 ataggctcgc ggcgcagctg gtactcgcac tcgctccttt ccacgggcgc atcgtggtgg
      421 tgaaccgcca ctgcgccaag gaatcgaccg gccacggctc gccgcttccg cacctcgtgc
      481 acggcggccc gggacgcgcg ggcgggggtg aggagatggg cggcatccgc ggcgtgctgc
      541 attacctgca gcgcaccgcc gtgcagggaa caccggatgt catcaccgcg acgagcgggc
      601 gctggacgaa aggcgcgcgc gagctcgacc ctgggacgca tccgttccgc aagcccttcg
      661 gctcactcgc gatcggagac acttttcact ccggcgagcg ccaggtcacg gtcgaggaca
      721 tcgagcgctt cgcggcgctc tccggcgacc ggttctacgc gcacatgggc gaggccgagg
      781 ccgcccgcaa tccgctgttc ggcgggcgcg tcgcgcacgg ctacttcctg gtggcggcgg
      841 ccgccggcct gttcgtcgat gcaccttacg gcccggtgct cgccaactat gggatcgacg
      901 gcctgcgctt cgtgaagccg gtaaagcccg gcgaccgcat tcgcgtacgc ctcacctgca
      961 aggaaaaatc gctgcgcgcg ggcgccggct acggcgaggt gcgctgggac acggccatca
     1021 ccaatcagaa cggcgaggcg gtggccaact acgacgtatt gacgatggtg agcgagaagg
     1081 cgattcccga cgcatagcgt ctgccgctac gttaagattt ggcgaggggg cccgatgcag
     1141 gagcgccatg cgcgtcggag cggatcgcgt gtctgatgcc gggcacagca cgcgcgccga
     1201 tgtcgtgatc atcggcgctg ggccggcggg gtcgaccgcg gctattcttc tggccgcgcg
     1261 cggctaccgc gtcgcgctgc tcgagaaggc gcatcacccg cgctttcata tcggtgagtc
     1321 gctgctgccc gcgaaccggc ctctgttcga gcaactgggc attgccgagc gggtgcgcgc
     1381 catcggcatg ctcaagtggg gcgcggagtt cgtgtcaccg tggcacggca ggagcgaggt
     1441 gttcgaattc gcctcggcgc gcaatcccga gctgcccttc agctaccagg tgcggcgttc
     1501 gcagttcgac gagatcctga tccggcgcgc cgcggaatgc ggcgcgacgg tggtcgaggg
     1561 gtgccgcgca cgggaggtcg agctcgagcg ccctgacggc ggggtgcgcg tcgtcgccga
     1621 gcacgaggac ggctcgaaat cggcctggca ggcccggttt ctcatcgatg cgtccgggcg
     1681 cgacaccttc cttgcgaatc acctgcacgt caagcaccgt aatccgcggc acaacagtgc
     1741 cgcgatctac gcgcacttcg ccggggtcga gcgccgcgcc ggcaagcgcg ccggcgacat
     1801 ctcgatctac tggttcgagt acggctggtt ctggctgatt ccgctcgccg acggcgttac
     1861 cagtgtcggt gcggtcgtat ggccgcacta tctgaagcag cgccggggtc ccatgccgga
     1921 gttcctgctc gcgaccattg cgcggtgtgc cccgctcgcc gagcgactga aggacgccac
     1981 gctggcctcc caggtcgagg ccaccggcaa ttactcctac agcgcatcgc tcagccacgg
     2041 ccgcaactac ctgctggtcg gcgacgcatt cgcgttcatc gatccggtgt tctcgaccgg
     2101 tgtgtggctg gcgatgcacg gcggggtggc agcctgcgaa gcggtcgaca cgtgtctgcg
     2161 gattcccgcg cgggcgggtg ccgcgctgcg cgcctacgat cgcacgacgc gtcgcgggcc
     2221 gcggctgctc tcgtggttca tctatcgggt caccaatccg gcattgcggg acctgttgat
     2281 ggcgccgcgc aacgcgctgc gcatgaggga tactctgctc tcggtcctgg cc
//
LOCUS       DATLKL010000364         1765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_83197, whole genome shotgun sequence.
ACCESSION   DATLKL010000364 DATLKL010000000
VERSION     DATLKL010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83197"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..819)
                     /locus_tag="VM713_04735"
     CDS             complement(160..819)
                     /locus_tag="VM713_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83607.1"
                     /translation="MPRSRLIAVPVSLVLLATGTARVPGADAPAKQNGAQRVIAPVFH
                     RLVAFSLPPPFKMAFERTTGNIYVREHVPEGETVDEWSRMITLSGVQGMSYSPEAKPQ
                     DYLQALARGFQWHCPDSYVALDLGPQPLAREPSFATVVSCGRVSSGGKGHSETSVMLA
                     IRGSDDFYVLQWTERGPDSSHPLALDGKSWSARLARLGPVQLCPIVPGEGPPYSSCAG
                     R"
     gene            complement(854..1255)
                     /locus_tag="VM713_04740"
     CDS             complement(854..1255)
                     /locus_tag="VM713_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83608.1"
                     /translation="MATDCPELERFVRGEIPAAEFPHREHVRMAFEMLKRHDFPETVL
                     HYSRALRAMTERAGKPQAFHQTVTIAFLSLIAERMEHGRADDFGAFARANPHLLEKSV
                     LSRWYRPERLSSEAARRTFLLPDPALQTHQN"
     gene            complement(1245..1502)
                     /locus_tag="VM713_04745"
     CDS             complement(1245..1502)
                     /locus_tag="VM713_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83609.1"
                     /translation="MKIITRTQTGVRLESHLLKVLKALATELDLSLGDLLEGIVLHAF
                     EGKTPFSAPTLRKIKTLRGVYGLDLTARDSHRMREAGKDGD"
BASE COUNT          302 a          583 c          601 g          279 t
ORIGIN      
        1 ccggcaagct cgcgatcgac gtgatcaacg gcagcgacct gctgcggcgc tgggtggacg
       61 ataccgcggc cacgccgggg gatctcgacg cgctggtacg caccgacgag gccgcatgga
      121 gcaccgagcg ccgcgcgctg ctgctctact gatggcggcc tagcgacccg cgcaactcga
      181 gtatggcggc ccctcgccgg gcacgatcgg acagagctgc accggcccga gccgtgcgag
      241 acgcgcactc caagacttgc cgtcgagcgc gagcgggtgg ctcgagtccg gcccgcgttc
      301 ggtccactgc aaaacgtaga aatcatccga gccgcggatc gccagcatga ccgaggtctc
      361 gctgtgcccc ttgccacccg agctgacacg cccgcaactc accacggtgg cgaatgacgg
      421 ctcgcgcgcc agcggctgcg gaccgagatc gagcgccacg tagctgtccg gacagtgcca
      481 ctggaagccg cgcgccagtg cctgcaggta atcctgcggc ttcgcttcgg gactgtaaga
      541 catcccctgc acgccgctca gcgtgatcat gcgcgaccac tcgtccacgg tctctccctc
      601 cgggacatgc tcgcggacgt agatgttgcc ggtggtgcgc tcgaatgcca tcttgaatgg
      661 gggcgggagc gagaaggcga ccaggcggtg aaacaccggt gctatcacgc gttgggcgcc
      721 gttttgcttc gcgggcgcgt ccgcgccggg aacacgggcg gtacccgtgg cgagcagcac
      781 gagcgatacc gggacagcaa tcaggcgcga gcggggcatg gccccaaaag catagcgcac
      841 ctgatcgctg gtcttaattc tggtgggtct gaagcgcagg gtccggcagc aggaaggtac
      901 gccgcgccgc ctctgagctc agccgctcgg ggcggtacca gcggctaaga accgacttct
      961 cgaggaggtg cggatttgcg cgtgcaaacg cgccgaaatc gtccgcgcgg ccgtgttcca
     1021 tccgttccgc gatcagcgac aggaaggcga tcgtcaccgt ctggtgaaag gcctgaggct
     1081 tgcccgccct ctcggtcatt gcgcgcagcg cgcggctgta gtgcagcacc gtctcgggga
     1141 agtcgtggcg cttgagcatc tcgaacgcca tgcgcacgtg ctcgcggtgc gggaattcgg
     1201 ctgcgggaat ctcgccgcgc acgaagcgct cgagctccgg gcagtcagtc gccatctttg
     1261 ccggcctcgc gcatgcggtg ggagtcgcgc gccgtcagat cgagcccgta cacgccccgc
     1321 agggtcttga tcttcctcaa cgtcggcgcc gagaaaggcg tcttgccctc gaaggcatgc
     1381 agcacgatgc cctcgagcag gtcgccgagc gacaggtcta gctcggtcgc gagcgccttc
     1441 agaaccttga gcagatgtga ctcgagtcgc acgccggtct gggtgcgggt aattatcttc
     1501 atgggcggat gttaccatgg ttttcggtac catggtacca acgtaccata aaccagggcg
     1561 gccagctgcc gggcgggcac ggtacggcgg gtcgcggact cccggcacaa ggcacggtca
     1621 ggctcgcgcc cgtgccggcg gcgcggcgga cagctttcca gccaacgcgg gcgattcccg
     1681 ataaaatagg cggctgcatg cagaccgccc gcgacatcac cggctatcgc aagtactggg
     1741 ccgagcgctt cggcacggcg ccgtt
//
LOCUS       DATLKL010000365         1536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_139787, whole genome shotgun sequence.
ACCESSION   DATLKL010000365 DATLKL010000000
VERSION     DATLKL010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139787"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1069
                     /gene="fdxH"
                     /locus_tag="VM713_04750"
     CDS             179..1069
                     /gene="fdxH"
                     /locus_tag="VM713_04750"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HVH83610.1"
                     /translation="MALQSLDIIRRSATTTPEPEVRGAHTGTVAKLIDVSKCIGCKAC
                     QVACMEWNDLRDEVGTNFGSYQNPADLSDQSWTLMRFSEYENPKGDLEWLIRKDGCMH
                     CADPGCLKACPSPGAIVQYANGIVDFHEENCIGCGYCVTGCPFDIPRISKRDHKAYKC
                     TLCSDRVAVGLEPACIKACPTGALVFGTKEDMQEHAAERVADLKSRGFEQAGLYDPPG
                     AGGTHVMYVLHHADQPQLYAGLAPNPHISGMVKVWKGAAKPLALAAMGLTLLAGFFHF
                     VRVGRNEVTPADEAAAEKGS"
     gene            1066..>1536
                     /locus_tag="VM713_04755"
     CDS             1066..>1536
                     /locus_tag="VM713_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HVH83611.1"
                     /translation="MSANPRRDSLVRYSAELRSNHWLNAIAFVLAALSGLALFHPALF
                     WLSNLFGGGPWTRILHPFIGLTVALLFLLLAAALWSDNRMKPADWQWLRQWRDVVEDR
                     EDRLPEVGRYNAGQKLLFYVLVIGIAGLFLSGLVIWRAYFSSYFPVGLIRLASLV"
BASE COUNT          259 a          527 c          488 g          262 t
ORIGIN      
        1 aaagccggtg cacaccgtgg gcatcccgct gcacgcggga tacaccggtc tcgtcaagcg
       61 gggcttcctg accaacaccc tcaccccggc cgttggcgac ggcaactcgc agacaccgga
      121 gttcaagtcg ttcctcgtga aggtagagaa ggcgtgaggc gcccggagag gcactgacat
      181 ggcgctgcaa tcgctcgaca tcatccgccg ctccgcgacg accacacccg agcctgaggt
      241 gcgcggcgcc cataccggca ccgtggccaa gctcatcgac gtgagcaagt gcatcggctg
      301 caaggcctgc caggtcgcct gcatggagtg gaacgacctg cgcgacgagg tcggcaccaa
      361 cttcggcagc taccagaacc cggccgacct ctcggaccag agctggacgc tgatgcgctt
      421 ctccgagtac gagaatccca agggcgatct cgaatggctg atccgcaagg acgggtgcat
      481 gcactgcgcc gatcccggct gcctcaaggc ctgcccgtcg cccggggcga tcgtgcagta
      541 cgccaacggc atcgtcgatt tccacgagga gaactgcatc ggctgtggct attgcgtgac
      601 cggctgcccc ttcgacattc cgcgcatctc gaagcgcgat cacaaggcct acaaatgcac
      661 gctgtgctcc gatcgcgtgg cggtcggcct cgagccggcg tgcatcaagg cctgccccac
      721 cggcgctctg gtcttcggca ccaaggagga catgcaggag catgccgccg agcgggtcgc
      781 ggacctcaag tcccgcggct tcgagcaggc aggcctctac gatccgcccg gtgccggcgg
      841 cacgcacgtg atgtacgtgc tgcatcacgc cgaccagccg cagctctacg ccgggctcgc
      901 gcccaatccc cacatcagcg ggatggtcaa ggtctggaag ggagcagcga aaccgctggc
      961 gctcgccgcc atggggctga cgctgctcgc tggattcttc cacttcgtgc gggtcgggag
     1021 aaacgaagtc acgcccgcgg atgaggcggc agcggagaag ggctcatgag cgccaaccca
     1081 cgccgtgatt cgctggtgcg ttacagcgcc gaactgcgta gcaaccactg gctgaatgcg
     1141 atcgccttcg tgctggcggc gctctcggga ctcgcgctgt ttcatccggc gctcttctgg
     1201 ctcagcaatc tcttcggcgg gggcccctgg acgcggatcc tgcacccgtt catcgggctg
     1261 accgtcgcgc tcctcttcct gctgctcgcc gcagcactgt ggagcgataa ccgcatgaag
     1321 cccgccgact ggcagtggct gaggcaatgg cgcgacgtcg tcgaggatcg cgaggaccgg
     1381 cttcccgagg tcggccgcta caatgccggg cagaagctgc tgttctacgt gctggtcatc
     1441 ggcatcgcgg gcctgttcct ctccggtctg gtcatctggc gagcctactt ctcgtcctac
     1501 ttccccgtcg gcctgatccg tctcgcctcg ctggtg
//
LOCUS       DATLKL010000366         2178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_60663, whole genome shotgun sequence.
ACCESSION   DATLKL010000366 DATLKL010000000
VERSION     DATLKL010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60663"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="VM713_04760"
     CDS             <1..1823
                     /locus_tag="VM713_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVH83612.1"
                     /translation="IRDAEFLMRRRDGQQLVILENSRPMRDAADLIIGYEGTIRDITA
                     RKRAEQAVFAEKERAQVTLQSIGDAVIATDAEGRIEYLNPVAESLTAWSLAEARGQPL
                     GAVLKLVNELTREPIGNSLAGALGRAESGGATDHTVLITRAGNEVAIQESAAPISDRA
                     GRVIGAVVVFHDVTRERRLKRALAYQASHDALTGLINRREFDNRLHAALLSAQRGEGA
                     YALLYIDLDQFKVVNDTCGHQAGDRLLRDVTGLLQTRVRATDTIARLGGDEFGVLLED
                     CTVEQASRIAEGIRQAIRDFRFVWGASTLSVGASVGIVQIRAETENVTQVMSAADIAC
                     YAAKDAGRNRIHVYDDDGVSNRHREMQWVSRVTRAAEDNLFELYFQPIVPLTPAAGPS
                     FHELTVRLRDDQTLVPPSEFIPAAERYNVMPVIDRWVVKRALELLKERQRLGVPLPLL
                     AVNLSGTSLNEQSFVDFVLQSVSEPAIAAALCFEITETAAVTSLTNARYLMSELKQRG
                     CKFSLDDFGTGVSSFVYLKSLPVDFLKIDGQFISHVAQDPVNRSMVEAIGRVGRALGI
                     ATVAECVETQVVLDELKRIGVDFAQGFYLARPQPIGELPG"
     gene            1901..>2178
                     /locus_tag="VM713_04765"
     CDS             1901..>2178
                     /locus_tag="VM713_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83613.1"
                     /translation="MRIEPRFLPAAFEVMSGEIVSCDFAQIDYDARLSGLRGAVVLNS
                     PGIRARAARGCALAAVALGLLTPAVIACAAARRPEPPPTDEQIAAELR"
BASE COUNT          353 a          764 c          723 g          338 t
ORIGIN      
        1 agatccgcga cgccgaattc ctgatgcgcc gccgcgacgg ccagcagctc gtgatcctcg
       61 agaactcgcg cccgatgcgc gacgccgccg atctcatcat cggctacgaa ggcacgatcc
      121 gcgacatcac cgcacgcaag cgcgccgagc aggccgtgtt tgccgagaag gagcgcgcgc
      181 aggtgacgct gcagtcgatc ggcgatgcgg tgatcgccac cgacgccgaa ggacgcatcg
      241 agtacctcaa tccggtcgcc gagagcctga ccgcctggag cctggccgag gcgcgcggcc
      301 agcctctggg ggcggtgctc aagctcgtca acgagctcac gcgcgagccg atcggcaatt
      361 cgctcgcggg cgccctcggg cgcgccgaga gtggcggcgc caccgaccac accgtgctca
      421 tcacgcgcgc cggcaatgag gtcgcgatcc aggagtcggc cgcacccatc tcggatcgcg
      481 ccggccgggt gatcggtgcc gtggtggtat tccacgacgt caccagggag cggcggctga
      541 agcgcgcgct cgcctaccag gcgagccacg acgcgctcac cgggctcatc aaccggcgcg
      601 aattcgacaa tcggctgcac gcggcgctgc tctccgcgca gcgcggcgag ggcgcctatg
      661 cgctgctcta tatcgatctc gaccagttca aggtcgtgaa cgacacctgc ggccaccagg
      721 caggcgaccg gctgctgcgc gacgtgaccg ggctgctgca gacgcgcgtg cgcgccaccg
      781 acacgatcgc gcgcctcggg ggcgatgagt ttggcgtgct gctcgaggac tgcaccgtcg
      841 agcaggcgag ccgcatcgcc gagggcatcc ggcaggcgat ccgcgacttc cgcttcgtat
      901 ggggggcgag cacgctgtcg gtgggcgcca gcgtcggcat cgtgcagatc agagccgaga
      961 ccgagaacgt cacgcaggtg atgagcgcgg ccgacatcgc ctgctacgcg gccaaggatg
     1021 ccggccgcaa ccgcattcac gtctacgacg acgacggcgt ctccaaccgc caccgcgaaa
     1081 tgcaatgggt gtcgcgcgtc acgcgcgcgg ccgaggacaa cctcttcgag ctctacttcc
     1141 agccgatcgt gccccttacg cccgccgccg gcccgagttt tcacgagctc acggtgcggc
     1201 tgcgcgacga ccagacgctg gtgccgccct cggagttcat ccccgcggcc gagcgctaca
     1261 acgtcatgcc ggtgatcgac cgctgggtgg tgaagcgcgc gctcgagctc cttaaggagc
     1321 gccagcgcct cggtgtgccg ctgccgctgc tggccgtgaa cctgtccgga acgtcgctga
     1381 atgagcagtc gttcgtggac ttcgtcctgc agagcgtcag cgagccggcg atcgcggccg
     1441 cactttgctt cgagatcacc gagactgcgg ctgtgacgag cctcaccaac gcccgctatc
     1501 tcatgagcga gctcaagcag cgcggctgca agttctcgct cgatgacttc ggcaccggcg
     1561 tgtcgtcgtt cgtgtacctg aagagcctgc cggtggattt cctcaagatt gacggccaat
     1621 tcatcagcca cgttgcccag gatcccgtca accgcagcat ggtcgaagcc atcggccggg
     1681 tcggccgcgc gcttgggatc gccacggtcg ccgaatgcgt cgagacgcag gtggtgctcg
     1741 acgagctcaa gcgcatcggc gtcgatttcg cccaggggtt ctacctggcg cgcccgcagc
     1801 cgatcgggga actgccggga tagcccgcac ggcgcagccg gcaaaaaccc ctgcgccgac
     1861 ctagctacgg agtgctcggc tactgaggcg attcggaccc gtgcgtatcg agccccgctt
     1921 tttgccggcg gcattcgaag taatgtccgg cgaaatcgtt tcctgcgatt tcgcccaaat
     1981 agactatgat gcgcgccttt cggggcttcg cggggcggtc gtgctcaact cacctggcat
     2041 tcgtgcgcgt gccgctcgcg gctgcgccct ggcggccgtc gcgttggggc tgctgacccc
     2101 cgcggtcatc gcctgcgccg ccgcgcgccg ccccgagcca ccacccacgg acgagcagat
     2161 cgccgcggaa ctgcgcga
//
LOCUS       DATLKL010000367         2594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_72144, whole genome shotgun sequence.
ACCESSION   DATLKL010000367 DATLKL010000000
VERSION     DATLKL010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72144"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VM713_04770"
     CDS             <1..591
                     /locus_tag="VM713_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HVH83614.1"
                     /translation="QAYMDGLSERARGRRTLGTVASVASFFLSRIDTLVDRLLDELSA
                     RGQPAARTLRGKAAIASAARAYEIFEATMATPQWQGLSAQGARPQRLLWASTSTKDPS
                     YSPIKYVEELVVPGTVNTMPLETLNAYRRLGRPELRLERHLAEGSDAREGLERLGVDL
                     EAVAAQLEREGVAKFVEPFDRLQKWLEDRRRAPKGS"
     gene            609..971
                     /locus_tag="VM713_04775"
     CDS             609..971
                     /locus_tag="VM713_04775"
                     /inference="COORDINATES: protein motif:HMM:NF045308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HVH83615.1"
                     /translation="METDAVRERLLKRRDELRQRASDASADLRHEADPLSADFAEQVT
                     QRENDDVLGAISHSARAELQQVEAALRRLAQGRYTTCSVCGGDIEPERLAAVPYTDKC
                     RACAEGVRPVPGRGRPGG"
     gene            993..1799
                     /locus_tag="VM713_04780"
     CDS             993..1799
                     /locus_tag="VM713_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HVH83616.1"
                     /translation="MAASGVITVTTDFGHQGPFVGVIKGCILTRFPAARIVDLTHEIV
                     VHWPAEAGFWLARAFPFFPAGTVHVAVVDPGVGTSRDILALEAGGHLFLAPDNGLLAP
                     VVTRIPDSRLVRLTPQGLARLGVAHPSATFHGRDIFAPVAAELAAGRCSAADLGEPVS
                     TLVPSWVDDPTVDPASVSGVVITIDHFGNLITNIDAALIERFRLPVVHAGNHAFPLLR
                     TYGDMKPGEYLALVNSFGVIEIARAEHNAAEGLGLSRGAPVVVRDGTDHA"
     gene            1950..2165
                     /locus_tag="VM713_04785"
     CDS             1950..2165
                     /locus_tag="VM713_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83617.1"
                     /translation="MAERDNEGFDLDEEFLNSPPEDGTDYDRLLDRVDKRVRNQGKRG
                     KAAWSRLEEVLADKKLEKDLQDFDDEL"
     gene            2273..>2594
                     /locus_tag="VM713_04790"
     CDS             2273..>2594
                     /locus_tag="VM713_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83618.1"
                     /translation="MTRTPSAWIVLKFGGTSVATLANWTNIAGVASERATSGARVLIV
                     HSAVSGVTDRLERLLDAARGDAYEAELKAIAERHQRLAADLGVQLGEEVERHFAELRE
                     IAAGI"
BASE COUNT          407 a          881 c          900 g          406 t
ORIGIN      
        1 caggcctaca tggacggcct gagcgagcga gcgcgcggcc ggcggacgct cggcaccgtc
       61 gcctcggtgg cgagcttctt cctgagccgc atcgatactc tggtcgaccg gctcctggac
      121 gagttgagcg cgcgcggaca gccggcggcg cgcacgctgc gcggcaaggc ggcgatcgcc
      181 agcgccgccc gcgcctacga gatcttcgag gccaccatgg cgacgccgca atggcagggg
      241 ctcagcgcgc agggcgcgcg cccacagcgg ctcctctggg cgtcgacctc caccaaggat
      301 ccaagctaca gcccgatcaa gtatgtcgag gagctggtcg tgcccgggac cgtgaacacg
      361 atgccgctcg agaccctcaa tgcctaccgg cgtctcgggc gcccggagct gcgtctcgag
      421 cggcacctgg ccgagggttc ggacgcccgc gagggcctcg agcgtctcgg cgtcgatctc
      481 gaggcggtcg ccgcgcagct ggagcgcgag ggcgtcgcca agttcgtcga gccattcgat
      541 cggctgcaga aatggctcga ggaccggcgg cgcgccccga agggctcctg agggcagcgg
      601 gagggtcagt ggaaaccgac gcggtacgcg agaggctgct gaaacgccgg gacgagctgc
      661 gccagcgggc gagcgacgcg agcgcggacc tgcggcacga ggcggacccg ctgtcggccg
      721 acttcgccga gcaggtgacg cagcgcgaga acgacgacgt gctgggcgcc atctcgcact
      781 cggcgcgcgc ggagctgcag caggtcgagg cggcgctgcg ccgtctcgcg caggggcgct
      841 acacgacctg ctcggtgtgc ggtggcgaca tcgagcccga gcgcctcgcg gccgtgccct
      901 acaccgataa atgccgcgcc tgcgcggagg gagtgcgtcc ggtgcccggc cgcggacgtc
      961 cgggcggctg actcggagcc tgcgagcgag ccttggcggc ctcgggcgtc atcaccgtta
     1021 ccacggactt cggacaccag ggcccgttcg tcggcgtgat caaggggtgc attctcacgc
     1081 gcttccccgc cgcgaggatc gtcgatctca cccacgagat cgtcgtgcac tggcctgcgg
     1141 aggcgggttt ctggctggcg cgcgcctttc ccttctttcc ggcgggcacc gtgcatgttg
     1201 cggtggtgga cccgggggtg gggacgtcgc gggacattct cgcgctcgag gcaggtggac
     1261 acctcttcct cgctcccgac aacgggctgc tcgccccggt cgtgacccgt attcccgatt
     1321 cgcgtctcgt ccggctcacg ccacaagggc tcgcacggct cggcgtcgcg cacccgagcg
     1381 cgaccttcca tgggcgggac atctttgcgc cggtcgccgc cgagctggcc gccgggcgct
     1441 gcagcgcggc ggaccttggc gagccggtga gcacgctcgt gccctcctgg gtcgacgatc
     1501 ctaccgtgga ccccgcgagc gtctcgggcg tcgtcatcac catcgaccac ttcggcaacc
     1561 tcatcaccaa catcgacgcg gccctcatcg agcgtttccg cctgccagtc gtccacgccg
     1621 gcaaccatgc ctttccgctg ctgcggacct acggcgacat gaaaccgggc gagtatctgg
     1681 ccctcgtcaa ttccttcggg gtcatcgaga tcgcccgggc cgagcacaac gcggccgagg
     1741 gcctggggtt gagccgcggc gctcccgtag tcgtcaggga tggcacggat cacgcttaag
     1801 ctgggctggt cttgggtctt gttctcaggg tgggagaccc ccattccggc aaatgttgat
     1861 cggcggcgcg aatcggctat agttcgcgcc ttgttccgtg ccgaataagc acgttagcct
     1921 ttgaattaat tcaggattta caccgagaga tggctgaacg ggacaacgag ggcttcgacc
     1981 tcgacgagga attcctgaac agtcctcccg aggacggcac cgattacgac cggctgctcg
     2041 atcgggtgga taagcgcgtc cgcaatcagg gcaagagggg caaggctgcc tggagtcgcc
     2101 tcgaggaagt cctcgccgac aagaagctcg agaaggacct ccaggacttc gacgacgagc
     2161 tctagcacaa gctcgggctg ctcactccaa gttcaagcaa tggcgatccc gaggcagcct
     2221 taagctgtca ggccgcggcg cggccttggc gctggctgcg gagaatcgag tcttgacccg
     2281 tacgccctct gcctggatcg ttctcaaatt cggtggcacg agcgtcgcca cgcttgccaa
     2341 ctggaccaac attgcagggg ttgcctccga gcgcgccacc agcggcgcgc gcgtcctcat
     2401 cgtgcactcg gccgtcagcg gcgtcaccga ccgcctcgaa cggctgctcg acgccgcgcg
     2461 cggcgacgcc tacgaggcgg agctcaaggc gatcgcggaa cgccatcagc gcctcgcggc
     2521 ggatcttggg gtgcagctcg gcgaagaggt cgagcggcac ttcgcggagc tgcgcgagat
     2581 cgcggctggc atcg
//
LOCUS       DATLKL010000368         1802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_15428, whole genome shotgun sequence.
ACCESSION   DATLKL010000368 DATLKL010000000
VERSION     DATLKL010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15428"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VM713_04795"
     CDS             <1..1101
                     /locus_tag="VM713_04795"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HVH83619.1"
                     /translation="PLSAVMAGVKDIRAAVESARAARAADGRATLLFLDEVHRFNKAQ
                     QDTFLPYLEDGTLTFVGATTENPSFEVISALLSRARIYVLKALTVEDLRAVLRAALTD
                     EERGLGREQLVAEPEALELIAQAADGDARRALNMLELAAGLAQAGTTPHTLDAALARE
                     VAAGGHRRFDKGGDQFYEQISALHKAVRGSDPDAALYWLCRMLDGGCDPRYIARRVVR
                     MASEDLGLADPRALALTLDAWEAYERLGTPEGELALATAVVYLACAPKSNAVYVAMAE
                     AMADVAEFGTLEVPLKLRNAPTRLMKELGYGRDYRYAHDEPDAFAAGERYLPDGMPDR
                     RYYRPVPRGLELKIAEALARLRRATPTPKGSE"
     gene            1098..>1802
                     /locus_tag="VM713_04800"
     CDS             1098..>1802
                     /locus_tag="VM713_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HVH83620.1"
                     /translation="MIDPKLLRNEPEEVARNLARRGYRLDVEALKALEDRRKPWAVEV
                     DRLRAEHNASAKAVGMAKGRGEDAAALIARGEALTTELAAAEGQLAAVQRELEQWQLG
                     LPNLLHASVPDGGDEGANAEVRRWGEPRRLAFKARDHVEIGERLGGLDFEAAARISGA
                     RFVVMRGPVARLHRALAQFMLDLHTREHGYVEVYVPYMVLPAALVGTGQLPKFEQDLF
                     AVRGEQDFHLIPTAEVP"
BASE COUNT          267 a          604 c          674 g          257 t
ORIGIN      
        1 ccgctctcgg ccgtgatggc cggggtcaag gacattcgcg cggcggtcga gagcgcgcgc
       61 gcggcacgcg ccgcggacgg gcgcgcgacg ctcctgtttc tcgatgaagt gcatcgcttc
      121 aataaggctc agcaggacac cttcctgccg tacctcgagg acggcacgct gaccttcgtc
      181 ggcgcgacca ccgagaaccc ctcgttcgag gtgatcagcg cgctgctgtc gcgggcgcgg
      241 atctacgtgc tgaaggcgct cacggtcgag gatctgcgcg cggtgctgcg tgcggcgctc
      301 accgatgagg agcgcggcct cggccgggag cagctggtgg cggagcccga agcgctcgag
      361 ctgatcgcgc aggcggccga cggcgatgcc cgccgcgcgc tcaacatgct cgagctcgcg
      421 gccggcctgg cgcaggccgg cacgacgccg cacacgctcg atgcggcgct ggcgcgtgag
      481 gttgccgccg gcggccaccg gcgcttcgac aagggcgggg atcagttcta cgagcagatc
      541 tcggcgctgc acaaggcggt gcgcggctcc gatcccgatg cggcgctcta ctggctgtgc
      601 cgtatgcttg acgggggctg tgatccgcgt tacatcgcgc gccgcgtggt gcgcatggcg
      661 agcgaggacc tggggttggc cgacccgcgc gcgctcgcgc tgactctcga tgcgtgggag
      721 gcctacgagc ggctcggcac accggaaggg gagctggcac tcgccaccgc ggtggtgtat
      781 ctcgcctgcg cgccgaagag caacgccgtg tacgtcgcga tggctgaggc gatggcggac
      841 gtggccgagt tcggcacgct cgaggtgccc ctcaagctgc gcaacgcgcc gacgcggctc
      901 atgaaggagc tcggctacgg ccgcgactac cgctatgcac acgatgagcc cgacgccttc
      961 gcggccggcg agcgctacct gccggacggc atgccggatc ggcgatacta tcgaccggtt
     1021 ccgcgcggcc tcgagctgaa gatcgccgag gcgctggcgc gcctgcgacg cgcgacaccc
     1081 acgccgaaag gtagcgagtg atcgatccga agctgctgcg taacgagccc gaggaagtgg
     1141 cccgcaatct ggcgcgccgc ggctatcggc tggacgtcga ggcgctcaag gccctggagg
     1201 acaggcgcaa accctgggcc gtcgaggtcg accggctgcg cgccgaacac aatgccagcg
     1261 ccaaggcggt cggcatggcc aaagggcgcg gggaggacgc ggccgcgctg atagcgcgcg
     1321 gcgaagcgct caccaccgag ctcgcggccg ccgagggcca gctggccgcg gtgcagcgcg
     1381 agctcgagca gtggcagttg gggctcccga acctgctgca cgcctcggtc cccgacggcg
     1441 gcgatgaggg tgccaatgcg gaagtgcggc gttggggcga gccgcggcgt ctcgccttca
     1501 aggcgcgcga tcacgtcgag atcggcgaac ggctgggcgg cctcgacttc gaggcggcag
     1561 cgcgcatctc gggcgcacgt ttcgtggtga tgcgcgggcc ggtcgcgcgg ctgcaccggg
     1621 cgcttgcgca gttcatgctc gacctgcata cccgcgagca cggctatgtc gaggtgtacg
     1681 tgccctacat ggtgctgccc gctgcgctcg tcggcaccgg acagctgccg aagtttgagc
     1741 aggacctgtt cgcggtgcgc ggcgagcagg atttccacct cattccgacc gcggaggtac
     1801 cg
//
LOCUS       DATLKL010000369         4162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_173438, whole genome shotgun sequence.
ACCESSION   DATLKL010000369 DATLKL010000000
VERSION     DATLKL010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173438"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="argH"
                     /locus_tag="VM713_04805"
     CDS             <1..1176
                     /gene="argH"
                     /locus_tag="VM713_04805"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HVH83621.1"
                     /translation="RLWDKGAPLDERVLRYTAGEDHALDDRLVPYDVRASIAHAEMLN
                     AQGLLPLADLDAIRDGLQELADEHARGLWHVELADEDGQTALERRLTERIGAAGARIH
                     LGRSRNDQVLTALRLYLREAIGDLESGALQVADALDELAVREGGTPLPGYTHMQQAMP
                     SSVPLWAGGFAAEIRDDAQGLAAAARRSSLSPLGSAAGYGTPGLPIDREATRAALGFA
                     AVQEPVTAVQLSRGKAEAQLLFEITLLMQDLGRLAADVLLYATHEFGFLTLADAFTTG
                     SSIMPQKRNPDLFELIRGHSATALGCLVEALGVCAKLPSGYNRDVQLLKFPLFRAIDL
                     ALETLAILPPAIAAVRFNAQAIRLDPTIHAAEEANRLAVREGIPFREAYRRIAAKLK"
     gene            1193..1753
                     /locus_tag="VM713_04810"
     CDS             1193..1753
                     /locus_tag="VM713_04810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HVH83622.1"
                     /translation="MLKLLGISGSLRRGSYNSALLRAAVRLMPADSLLEVGSIAGIPL
                     YNGDVEAQGIPAVVAQLKEAVVAAAGVLLVTPEYNNSIPGVFKNAIDWLSRPNTDIRR
                     VFGAKPFAIMGASPGGYGTNLSQVAWLPVLRTLGTELWTGGRLQLSRAATVFDAAGNL
                     KDPGVEQQLQQFLAGYVAFVRRAVSA"
     gene            complement(1767..2102)
                     /locus_tag="VM713_04815"
     CDS             complement(1767..2102)
                     /locus_tag="VM713_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83623.1"
                     /translation="MSPLNNSGVLEYLRGPPLEADDDTVNAWYAQWIARGFAALEQDA
                     ANTSGDGRHMFGTALTLADVLLVPQMYNARRYRCDLTPYPLLRGICAHLEALPAFARA
                     APEAQEGAQ"
     gene            complement(2199..2414)
                     /locus_tag="VM713_04820"
     CDS             complement(2199..2414)
                     /locus_tag="VM713_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVH83624.1"
                     /translation="MKLYHYFRSSASYRVRIALNVKGLAYEAVAVDLRPQVHGQRAAE
                     FLALNPQGLVPVLIDGERTLTQSLAII"
     gene            complement(2411..3103)
                     /locus_tag="VM713_04825"
     CDS             complement(2411..3103)
                     /locus_tag="VM713_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVH83625.1"
                     /translation="MSEFAFPPSAQPAVAIEGGGRFPVRRIYCVGLNYADHIREFGRD
                     PERSPPVFFMKPADALVANGAAVPYPPRTANFHYEIELVVAIGRAGSGIARSSALEHV
                     FGYAAGNDLTRRDLQRAAKERGEPWDIAKGFDHSAPVAAIRPASRGHLARGRIWLTVN
                     GERRQESDLSQMLYDVPHLVAELSTLYTLMPGDLIFTGTPSGVGPVQPGDRLEGGIDG
                     LEVLRNTIVAPA"
     gene            3148..3579
                     /locus_tag="VM713_04830"
     CDS             3148..3579
                     /locus_tag="VM713_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HVH83626.1"
                     /translation="MGIWRVTATPAELNERGSRTLSDFLGIRITEIGPDFLRATMPVN
                     ERTHQPFGVLHGGASVALAETVGSLAAMLCIEPGYLALGQDINANHLRSISSGLVIAT
                     ARPFHLGRTSHVWHIEIRDEQDRLVCVSRLTMAVVERRPKS"
     gene            3608..4036
                     /locus_tag="VM713_04835"
     CDS             3608..4036
                     /locus_tag="VM713_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83627.1"
                     /translation="MPDSATPPMAAPAPRVDESLVTYTNVIYALHSLSVLIGLTSFHT
                     VVGAFIWGLPSIVAVIMNYARRSATHGTYLESHFRWQIRTFWYAVLWSVVIWAVSVPL
                     MVILIGFPLLILGHYALAIWIIYRIARGWLALRDQRPMYV"
BASE COUNT          628 a         1460 c         1435 g          639 t
ORIGIN      
        1 cgcctgtggg acaagggcgc gccgcttgat gagcgggtgc tgcgctacac ggcgggcgag
       61 gaccatgcgc tcgatgaccg cctggtgccc tacgacgtgc gcgcctcgat cgcgcacgcc
      121 gagatgctga atgcgcaggg actgctgccg ctcgccgacc tcgatgcgat ccgcgacggc
      181 ctgcaggagc tcgccgacga gcacgcgcgc gggctctggc acgtggagct cgccgacgag
      241 gatggccaga cggcgctcga gcggcgcctg accgagcgca ttggggccgc cggggcgcgc
      301 attcacctcg gacgctcgcg caacgaccag gtgctcaccg cgctgcgcct gtatctgcgc
      361 gaagcgatcg gcgacctcga gagcggcgcg ctccaggtgg cggatgcgct cgatgagctc
      421 gccgtgcgcg agggcggcac tccgctcccc ggctacacgc acatgcagca ggcgatgccg
      481 agctcggtgc cgctctgggc ggggggcttc gccgccgaga tccgcgatga cgcgcagggc
      541 ctcgccgcgg cggcgcgccg cagctcgctc agcccgctgg gatcggcggc cggctacggc
      601 accccgggac tgcccatcga ccgcgaagcg acgcgcgccg cgctcggctt cgccgcggta
      661 caggagccgg tgaccgccgt gcagctctcg cgcggcaagg cggaggcgca gctgctcttc
      721 gagatcacgc tcctcatgca ggacttgggc aggctcgccg cggacgtgct gctgtacgcg
      781 acgcacgagt tcggctttct cacactcgcg gatgccttca ccaccggctc ctcgatcatg
      841 ccgcagaaga gaaatcccga cctgttcgag ctgatccgcg gccactcggc cacggcgctc
      901 ggctgcctcg tcgaggcgct cggggtgtgc gccaagctcc cctcgggcta caaccgcgac
      961 gtgcagctgc tcaaatttcc gctgttccgc gccatcgatc tggcgctcga gacgctcgcc
     1021 atcctgccgc cggccatcgc cgccgtgcgc ttcaacgccc aagcgatcag gctcgatccc
     1081 acgatccacg ccgccgagga agcgaaccgc ctggcggtgc gcgagggtat cccgttccgc
     1141 gaggcctacc ggcgcatcgc ggcgaagctc aaataaaagc ccaactcaac tcatgctcaa
     1201 gctgctgggg atatcgggaa gcctgcgccg cggctcctac aactcagcgc tgctgcgcgc
     1261 cgcggtgcgc ctcatgcccg cagactcgct gctggaggtc ggcagcatcg cgggcatccc
     1321 gctctacaat ggcgacgtcg aggcgcaggg gatccccgcg gtggtggcgc aactcaagga
     1381 agccgtggtc gccgccgccg gcgtgctgct ggtcactccg gaatacaaca acagtatccc
     1441 cggggtcttc aagaacgcga tcgactggct gtcgcgaccg aacaccgaca tcaggcgcgt
     1501 gttcggggcg aagccgttcg cgatcatggg cgcatcgcct ggcggctacg gcacgaacct
     1561 ctcgcaggtc gcgtggctcc cggtgctgcg cactcttggc acggagctct ggaccggcgg
     1621 gcggctgcag ctgtcgcgcg ccgccacggt gttcgatgcg gccggcaacc tcaaggaccc
     1681 gggcgtcgag cagcagctgc agcagttcct cgcgggctac gtcgctttcg tgcggcgggc
     1741 ggtgtccgcc tagcggaggc acgcagctac tgcgcgccct cctgggcctc cggggcggca
     1801 cgcgcgaacg ccggcagcgc ctcgaggtgc gcgcagatcc cgcgcaacag cggatacggc
     1861 gtgaggtcgc accggtagcg gcgcgcgttg tacatctgcg gcaccaacag cacatcggcg
     1921 agcgtcaatg cggtgccgaa catgtggcgg ccgtcgccac tcgtgttcgc cgcatcctgc
     1981 tcgagcgccg cgaagccgcg ggcgatccac tgcgcgtacc aggcgttcac ggtatcgtca
     2041 tccgcctcga gcggcgggcc gcgcaggtac tcgagcaccc cggagttgtt gagcgggctc
     2101 atctcgcagg cgatggcgag cgcgagagcg cgcacccggg cccgtgcggc ggcgccctgg
     2161 ggcagcagcg gcggccgcgg gtgagtctcg tcgagatact agatgatcgc gagcgactgg
     2221 gtgagcgtgc gctcgccgtc gatgaggacc ggcacgaggc cctgtgggtt gagcgcgaga
     2281 aactccgccg cgcgctgccc gtgcacctgc ggccgcaggt cgaccgccac cgcctcgtac
     2341 gcgaggccct tcacattgag cgcgatgcgt acccggtagg aggcggagct gcggaagtag
     2401 tgataaagct tcatgccggt gcgacgatgg tgttgcgcag cacctcgagc ccatcgatcc
     2461 cgccctcgag ccggtcgccg ggttgcacgg gcccaacgcc ggacggcgtg ccggtgaaga
     2521 tgaggtcccc cggcatgagc gtgtagagcg tcgacagctc ggcgacgaga tgcgggacgt
     2581 cgtagagcat ctgactcagg tccgactcct ggcggcgctc gccgttcacg gtgagccaga
     2641 tccgcccgcg cgcgaggtgg ccgcggctcg cggggcggat cgcggcgacc ggcgcggagt
     2701 gatcgaatcc cttggcgatg tcccagggct cgccgcgctc cttggcggcc ctctgcagat
     2761 cgcggcgcgt caggtcgttg ccggcggcat agccgaacac gtgctcgagc gcgctcgagc
     2821 gcgcgatgcc gcttccggcg cggccgatcg ccaccaccag ctcgatctcg tagtggaagt
     2881 tcgcggtgcg cggcggataa ggaaccgccg caccgttcgc gaccagggcg tccgcgggct
     2941 tcatgaagaa caccggcggg gagcgctccg ggtcgcgtcc gaactcgcgg atgtggtccg
     3001 cgtagttgag gcccacacag tagatgcgac gcaccgggaa gcgcccgccc ccctcgatgg
     3061 cgacggccgg ctgtgcggat ggcggaaaag caaactcgct catgggcctt gaagattgcc
     3121 accgcgcgaa cggctagact agctcacatg ggcatctggc gcgtgaccgc gacccccgcg
     3181 gagctcaacg agcgcggcag ccgcacgctg tccgacttcc tcggcatccg catcaccgaa
     3241 atcggcccgg acttcctgcg cgccaccatg ccggtgaacg agcgcaccca tcagcccttc
     3301 ggcgtgctcc acggcggggc gtcggtcgcg ctcgcggaga ccgtggggag cctcgcggcg
     3361 atgctgtgca tcgagccggg ctacctggcc ctcggccagg acatcaacgc caatcacctg
     3421 aggagcatct cctccggact cgtcatcgcc acggctcggc ccttccatct cggcaggacc
     3481 agccacgtat ggcatattga gatccgcgac gagcaggacc gcctggtgtg cgtgtcgcgg
     3541 ctcacgatgg ccgtggtcga gcggcgcccg aagagctgag cgcaccagcg cccgcgagag
     3601 gtacgtcatg cccgacagcg caaccccgcc gatggctgcc ccagctcccc gcgtcgacga
     3661 gtcgctcgtc acctacacca acgtcatcta tgcgctgcac tcgctgtccg ttctgatcgg
     3721 gctcacgagc ttccacactg tggtgggagc tttcatatgg ggcctgccgt cgatcgtcgc
     3781 ggtgatcatg aattacgcac gccgctcggc gacccacggc acctatctcg agtcgcactt
     3841 ccgctggcag atccgcacct tctggtatgc ggtgctgtgg agcgtggtca tctgggccgt
     3901 gtcggtcccg ctcatggtga tcctgatcgg cttcccgctc ctcatactcg ggcactatgc
     3961 gctggcgatc tggatcatct atcgcatcgc gcgcgggtgg cttgcgctgc gcgaccagcg
     4021 tcccatgtac gtatgagggg cgcctggccg ctgctcgcgc tggctgggct cgcagccggt
     4081 ggctgcggcc agaagggacc cctttatctt ccggacaaag ttggcaaggt ggtgacgagc
     4141 gcgccggccg ccgcgcagtc cg
//
LOCUS       DATLKL010000370         1854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_128472, whole genome shotgun sequence.
ACCESSION   DATLKL010000370 DATLKL010000000
VERSION     DATLKL010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128472"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..615
                     /locus_tag="VM713_04840"
     CDS             82..615
                     /locus_tag="VM713_04840"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HVH83628.1"
                     /translation="MALWKDAPTGTVTGAGTVSPGPGGVVSTPYAREPEKTNVAPLSP
                     DVPRRAAAPPRQEVKESFLSAELTIEGKIAGSGHVRIAGRFKGDVQVDGNLSLDAGAR
                     LEGHVKASVVSVGGELIGNIENAKRVDLLETGVINGDVKAGALTVAAGSRMRGQVEFG
                     YEVEGRSKAESKSFGNA"
     gene            612..1139
                     /locus_tag="VM713_04845"
     CDS             612..1139
                     /locus_tag="VM713_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83629.1"
                     /translation="MSVARPDTAGKTRTCPHCKATILESLTICPGCLHHLRFDQPAAK
                     RQVSAVSALRVEGVIEHPTTAKGPWEYFVVIAVRNEHGEEITRQVVNVGALQHNDKRT
                     FALSVEVMPSDSLAVPKKPAAAQAPGPAPRVPAPKAPLPRPPAASSAAKPSPAAATAG
                     VVAKPGAGSAAPPKR"
     gene            complement(1167..>1854)
                     /locus_tag="VM713_04850"
     CDS             complement(1167..>1854)
                     /locus_tag="VM713_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83630.1"
                     /translation="DFIRLYNHDQILRTEDFYLGTLATLRVGWADSALGSSRSALIVQ
                     GTAGRGFRDGGTSTLILGSAFSGRLENGTLQNGVADASVRYYAEQSSNWLFFTTLVGT
                     KAWRPDLDNQVLLGGDNGLRGYPLRYQDGTARALWTIEQRYFTDWFIFRLFRAGAAIF
                     FDAGRSWGEAPLAQPSLGLLKDAGFGLRFGNARSGLGNVIHVDVAFPFERANGTIRGA
                     QFLVTTQQSF"
BASE COUNT          332 a          628 c          636 g          258 t
ORIGIN      
        1 cgtgtctgca cgcagagaca ctggtcgccc cgcgaccggg cgggtaagtt ccattgttca
       61 atcatttggg gggcatatct catggcgctt tggaaagacg cacctaccgg aaccgtaacc
      121 ggagccggaa ccgtatcccc cggccccggc ggcgtggtga gcacgccgta tgcacgggag
      181 ccggagaaga ccaacgtagc tccgctcagc ccggatgtgc cgcgccgcgc ggctgcaccg
      241 ccgcggcagg aagtgaagga atccttcctc tctgccgagc tcaccatcga aggcaagatc
      301 gccgggtccg gccacgtgcg cattgccggc cgcttcaagg gcgacgtgca ggtcgatggg
      361 aacctgagcc tcgatgcggg agcccgcctc gaaggccacg tcaaggcgag cgtcgtgagc
      421 gtcggaggcg agctcatcgg caacatcgag aatgccaaga gggtggacct cctcgagacc
      481 ggcgtcatca acggcgacgt caaggcagga gcgctcacgg tcgccgcagg ctcgcgcatg
      541 cgcggccagg tcgagttcgg ctacgaggtc gagggccgct ccaaagctga gagcaagagc
      601 ttcggcaacg catgagcgtc gctcgccccg acaccgcggg caagacgcgc acctgccccc
      661 actgcaaggc gacgattctc gagagcctga cgatctgccc ggggtgcctg caccacctgc
      721 gcttcgacca gccggcggcg aagcgacagg tgtcggccgt ttccgcgctg cgcgtggagg
      781 gagtgatcga gcatcccacg acagccaagg ggccctggga gtacttcgtc gtgatcgcgg
      841 tgcgtaacga gcacggcgag gagatcacgc ggcaggtcgt caatgtcggc gcgcttcagc
      901 acaacgacaa gcgcaccttc gctctctcgg tcgaggtgat gccgtcggac tcgctcgcgg
      961 tgcccaagaa gcccgccgcg gcgcaagctc ccggcccggc cccccgcgtc cccgccccca
     1021 aggcgccgct tccgcggccg cccgccgcga gctcggcagc gaagccgagc ccggcagctg
     1081 cgacggccgg cgtcgtggcg aagcccggcg caggctccgc ggcgccgcct aagcgctagc
     1141 tcgacgaagt cagccgagac ggtgactcag aaactctgct gagtcgtcac gagaaactgc
     1201 gcgccacgga tcgtgccgtt cgcccgctcg aacggaaagg caacatcgac gtggatgaca
     1261 ttgccgagcc ctgatcgcgc attcccaaat cgcagcccaa agcctgcgtc cttgaggagc
     1321 ccgagcgagg gctgcgcaag cggggcttcg ccccagctgc gaccggcgtc gaagaagatc
     1381 gcggctcccg cccggaagag ccggaagatg aaccagtcgg tgaagtagcg ctgctcgatc
     1441 gtccacaagg cgcgcgcggt gccgtcctgg taacgcagcg ggtagccccg caggccgttg
     1501 tccccgccga gcagcacctg gttgtcgaga tcgggccgcc aggccttggt gccgaccagc
     1561 gtcgtgaaga acagccagtt cgagctctgc tcggcgtaat agcggaccga ggcatccgcg
     1621 acgccgttct ggagcgtgcc gttctcgagg cgccccgaga aagcgctccc gaggatgagc
     1681 gtcgaggtgc cgccgtcgcg aaaaccgcgg cccgcggtgc cctggacgat gagcgcgctg
     1741 cggctcgatc cgagcgccga gtccgcccag ccgacgcgta gcgtcgcgag ggtcccgagg
     1801 tagaagtcct cggtgcgcag gatctggtcg tggttgtaga gccgtatgaa gtcg
//
LOCUS       DATLKL010000371         2046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_139845, whole genome shotgun sequence.
ACCESSION   DATLKL010000371 DATLKL010000000
VERSION     DATLKL010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139845"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1602)
                     /locus_tag="VM713_04855"
     CDS             complement(<1..1602)
                     /locus_tag="VM713_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HVH83631.1"
                     /translation="MTGTQFGGIVLGLIVVAVVAAIIVWLFHWLYLRSSKERAFVRTG
                     MGGQKVVMNGGAFVLPIVHDVIPVNMNTLRLEVSRGRDKALITKDRMRVDVVAEFYVR
                     VRAETAAIASAAQTLGQRTMQPELLKELLEGKFVDALRTVAAEMAMEELHERRGEYVK
                     RVRAAVAEDLLQNGLELESASLTQLDQTQMEFFNPSNAFDAEGLTRLTEQIERRKKIR
                     NDIEQDTMIQIRNKNLEAEKLSLEIERDSEHARLSQERELEIARAEQRAALASERAEK
                     QQLAERAEITAREQVERARIETDRMLEGERIAKERAIEAAEIERRQAIELAEQKRAIA
                     VAGQSKALSEAQAGADRARALAAAEEEKVFTARETEMAARRKAIELIAAAQEAEREAL
                     RLKVGAEAEKLAAADRGLAIRAQAEAEAESEKIRALAQQLSNEINAEGVRLMNEAQNV
                     LTLEARGSAARLKLIDKLDAIIREQVKPMERIEGIKILHVDGLGSGSGGYSAAHEGGS
                     FADQIVNSALRFRAQAPLIDELLKEI"
     gene            complement(1625..>2046)
                     /locus_tag="VM713_04860"
     CDS             complement(1625..>2046)
                     /locus_tag="VM713_04860"
                     /inference="COORDINATES: protein motif:HMM:NF020927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HVH83632.1"
                     /translation="CDLLTTPYVFDAEQARAMTRAGADIIVCHMGLTTGGAIGAETAI
                     SLEDCVPRIEEWAAAAKRVRKDVIVLCHGGPIARPEDAAFILERCGECHGFYGASSME
                     RLPTERAIVDQTRHFKGIARRERAQPVQNGWRGESRR"
BASE COUNT          282 a          726 c          643 g          395 t
ORIGIN      
        1 gatctccttc agcagctcgt cgatgagcgg cgcctgagcg cggaagcgaa gcgctgaatt
       61 cactatctgg tcggcgaagc tgccgccctc atgcgctgcg ctatagccgc cgctgccgct
      121 gccgaggccg tcgacatgca ggatttttat gccctcgatc cgctccatcg gcttcacctg
      181 ctcgcggatg atggcgtcga gcttgtcgat cagcttcaag cgcgcggccg agccgcgcgc
      241 ctcgagcgtg agcacgttct gcgcctcatt cataagccgc acgccctcgg cattgatctc
      301 gttcgagagc tgttgcgcca gcgcccggat cttctcggac tcggcctcgg cctcggcctg
      361 cgcccggatc gcgaggccgc ggtcggcggc ggcgagcttc tccgcctcgg caccgacctt
      421 gagtcggagc gcctcgcgct cggcctcctg cgccgcagcg atgagctcga tggccttgcg
      481 ccgtgctgcc atctcggtct cacgcgcggt aaagaccttt tcctcttcgg cggcggcgag
      541 cgcgcgggcg cggtcggcgc cggcctgcgc ctctgatagc gccttcgact gtcccgccac
      601 ggcgatagcg cgcttctgct cggcgagctc gatcgcctgg cggcgctcga tctcggcagc
      661 ctcgatcgcg cgctctttgg cgatgcgctc cccttccagc atgcggtcgg tttcgatgcg
      721 cgcccgctcg acctgctcgc gcgcggtgat ttcggcgcgc tcggcgagct gctgcttctc
      781 tgcgcgctcg ctcgccagcg ccgcgcgctg ctcggcgcgc gcgatctcga gctcgcgctc
      841 ctgcgagagc cgtgcatgct ctgaatcgcg ctcgatttcg agcgagagct tttccgcctc
      901 cagattcttg ttgcggattt ggatcattgt gtcctgctca atgtcattgc ggatcttttt
      961 gcggcgctcg atctgctcgg tcaaacgcgt cagaccctcg gcgtcgaagg cgttcgacgg
     1021 gttgaagaac tccatctgcg tctggtcgag ctgggtgagc gaggcagatt cgagctcgag
     1081 cccattctgc agcagatcct ccgccactgc ggcgcgcacg cgcttcacgt attcgccgcg
     1141 tcgctcgtgt agctcctcca tcgccatctc ggcggccacc gtgcgcagcg cgtcgacgaa
     1201 cttgccctcg agcagctcct tcagcagctc cggctgcatc gtgcgctggc ccagcgtctg
     1261 tgccgcgctg gcgatcgctg cggtctcggc gcggacacgc acgtagaact cggcaacgac
     1321 gtcgacccgc atacggtcct tggtgatgag cgccttgtcg cggccgcgcg atacctcgag
     1381 gcggagcgtg ttcatgttga ctgggatcac gtcgtgcacg atcgggagca cgaaggcgcc
     1441 tccgttcatc acgaccttct ggccgcccat gccggttcgc acgaaggcgc gctccttgct
     1501 cgatcggaga taaagccagt ggaagagcca gacgatgata gcggcaacga ccgcgaccac
     1561 aatgagcccg aggacgatgc ctccgaattg tgtgccggtc atatcaacct cccgtaccct
     1621 atctctaccg tctcgactca ccgcgccagc cgttctggac tggctgggcg cgctcgcgcc
     1681 gcgcgatgcc cttgaagtgc cgcgtctggt cgacaatcgc ccgctccgtc ggcagccgct
     1741 ccatgctcga ggcgccgtag aagccgtggc actcgccaca gcgctcgagg atgaaggcgg
     1801 cgtcctccgg ccgcgcaatt ggcccaccat ggcacagcac gatcacgtcc ttgcggacgc
     1861 gcttggccgc cgccgcccat tcctcgatcc gcggcacaca atcctcgagc gagatcgcgg
     1921 tctcggcgcc gattgcgccg ccagtggtga gccccatatg gcagacgatg atgtccgcgc
     1981 ccgcccgcgt catggcgcgc gcctgctcgg cgtcgaagac atagggcgtg gtcagcaggt
     2041 cgcacg
//
LOCUS       DATLKL010000372         1682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_151109, whole genome shotgun sequence.
ACCESSION   DATLKL010000372 DATLKL010000000
VERSION     DATLKL010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151109"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1186)
                     /gene="rodA"
                     /locus_tag="VM713_04865"
     CDS             complement(47..1186)
                     /gene="rodA"
                     /locus_tag="VM713_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HVH83633.1"
                     /translation="MIYEEVPYGSRTRRTFSGAARVLLALQIDGPLVVGLGLIAGFGL
                     IVLYSASGQSTPTVVRTVMRIVLGTAAMLLLARVNPNFLRRSTPWLYAAGVFLLLIVA
                     AFGHTGLGAQRWLNLGFFRFQPSELMKLAVPMTCAWYLYERPLPPGMLSLLVLAALIF
                     LPVGLVAIEPDLGTAALIAVAGALVIVLAGLRVRVMVALLAVAAACAWFGWSFMHDYQ
                     RKRVLTFLNPQTDPLGAGYHIIQSQIAIGSGGVFGKGWMNGSQAQLEFLPERSTDFIF
                     AVVGEEFGLLGLLLLLLLYLFVVARSIFLATQTQDTFARLLAGSIALTFFVYVVINAG
                     MVTGLLPVVGVPLPLVSYGGSSVVTLLAGFGILMALYSRRKLVGS"
     gene            complement(1183..>1682)
                     /locus_tag="VM713_04870"
     CDS             complement(1183..>1682)
                     /locus_tag="VM713_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HVH83634.1"
                     /translation="GAVRRLAPVPGGEVTAIAPADWERVIRDMVGVTQRGTAAGIGAH
                     APYIFGGKTGTAQVFTVARTEKYNAKNINERLRDHSWFICFAPADSPRIALAVIEENG
                     GAGASAAAPIARKVLDAFMLDAEGKVKSAPARPAPAAAPAAPAPIPAAPPAPAPAVTT
                     AANHT"
BASE COUNT          301 a          555 c          608 g          218 t
ORIGIN      
        1 caagcaaagc gtgacgggag tagatcaagc tggatccgtg ctcgctttac gagcctacga
       61 gcttccgccg tgagtacagc gccatgagaa taccgaagcc cgcgaggagc gtcacgaccg
      121 agctgccgcc gtagctcacg agcggcaacg gcacgccgac caccgggagg agccccgtca
      181 ccatgccggc gtttatcacc acgtagacga agaaggtcag tgcgatgctt cccgcgagga
      241 gccgcgcgaa cgtgtcctgc gtctgcgtgg cgagaaagat cgagcgcgca acgacgaaca
      301 gatagaggag cagcaacagc agcaggccca gcaggccgaa ctcctcaccg accacggcga
      361 agatgaagtc ggtcgaacgc tccggcagga attcgagctg cgcctggctg ccgttcatcc
      421 agcccttgcc gaagacgccg cccgagccga tcgcgatctg cgactggatg atgtgatagc
      481 cggcgcccag cggatcggtc tgcgggttga ggaaggtcag tactcgcttg cgctggtagt
      541 cgtgcatgaa gctccagccg aaccaggcac aggccgccgc aaccgccagg agcgccacca
      601 tcacccgcac gcgcaggccc gcgagcacga tcacgagcgc ccccgcgacc gcgatcagcg
      661 ccgcggtgcc gagatcgggc tcgatcgcga cgagtccgac tggcaggaag atcagcgccg
      721 cgagcacgag cagcgacagc atgcccggtg gcagcgggcg ctcgtacaga taccaggcgc
      781 aggtcatcgg cacggccagc ttcatgagct cggagggctg gaagcggaaa aatccgagat
      841 tgagccagcg ttgcgcgccg agcccggtgt ggccgaacgc cgccacgatg agcagcagga
      901 acaccccggc ggcgtacagc cacggtgtcg agcggcgcag gaagttggga ttgacgcgcg
      961 ccagcagcag cattgccgcc gttccgagga cgatgcgcat gacggtgcgc acgaccgtag
     1021 gggtgctctg accggaggcg ctgtagagga cgatgagccc gaagcccgcg atcaacccga
     1081 ggcctacgac gagcgggccg tcgatctgga gcgccagcag cacccgcgcc gccccgctga
     1141 acgtgcgccg cgtgcgcgag ccgtagggga cttcctcgta gatcatgtgt ggttcgccgc
     1201 ggtggtcacc gcgggggcgg gggccggggg cgcagccggg atcggggcgg gggcggcggg
     1261 cgcggcggcc ggggccgggc gcgccggagc ggacttgacc ttgccttcgg cgtcgagcat
     1321 gaacgcgtcg agcaccttgc gggcgatggg ggcggcggca ctcgcgccgg cgccgccgtt
     1381 ctcctcgatc acggcgagcg cgatgcgcgg ggagtctgcc ggcgcgaagc agatgaacca
     1441 ggaatgatcg cgcagccgct cgttgatgtt cttggcgttg tatttctcgg tgcgcgccac
     1501 cgtgaagacc tgtgcggtgc cggttttgcc gccgaagata tagggggcgt gcgcgccgat
     1561 ccccgcggcg gtgccgcgct gcgtgacgcc caccatgtcg cggatcacgc gctcccagtc
     1621 ggccggggcg atggcggtca cctcgccccc cggcaccggg gcgagccgcc gcaccgctcc
     1681 gc
//
LOCUS       DATLKL010000373         5753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_94668, whole genome shotgun sequence.
ACCESSION   DATLKL010000373 DATLKL010000000
VERSION     DATLKL010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94668"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2184
                     /locus_tag="VM713_04875"
     CDS             <1..2184
                     /locus_tag="VM713_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH83635.1"
                     /translation="DQVIDRTITIQASVRDVELSLLIAILLVVTVIFLFLRSFWATAI
                     PGITVPLALFGTAALMYLVGYSLDNLSLMALTIAVGFVVDDAIVMLENIYRHIEEGLT
                     PYQAAVKGAGEIGFTILSISISLIAVFIPLLLMSGIIGRLFREFAVTVTMTIAVSAVV
                     ALTLSPMLGALYLRDEKHARHGRAYQTLERGFEWLLDHYTRGLDFVLRHQGATLTSFL
                     ITVGVAVALYAIVPKGFFPQQDTGIIAGLTDAPQDVSFNQMMRLQRNLTGIVARDPAV
                     AGYAAFVGGGRPLNNGFVIIGLKPRDERDVTADQVIERLRRQINSVPDGTLFLQAQQD
                     LNVGGRLTRTQYQYTLQDPNLEELNTWAPRLLDKLKKLPQLRDVASDQQSSSTKLALV
                     IDRDQAARFGIQPALIDNTLYDAFGQRQVTQYFTQLNSYHVVLEVTPSLLGDPDTLRK
                     IYIKSPLTGQQVPLSTFTRYDTGHTSYLSVNHQGQFPAVTLSFNLAPGVALGDAVTAI
                     QKTAAAIHLPAAILGTFQGTAQAFKDSLSTAPLLILAALLAVYIILGVLYESYIHPLT
                     ILSTLPSAGVGALLMLMLFHFDLSVVGLIGIILLIGLVKKNGIMMVDFALHAERNEGL
                     EPQAAIRKACLLRFRPIMMTTMAAILGALPLMLGRGTGSELRQPLGFTMVGGLVLSQM
                     LTLFTTPVVYLYLDRLSVRWARKHPHRDVGGGAVAAADAAQASAP"
     gene            2181..2861
                     /locus_tag="VM713_04880"
     CDS             2181..2861
                     /locus_tag="VM713_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVH83636.1"
                     /translation="MKILLVEDNERVARFVTKGLREAGHTVEHAGNGRDGMFLAASES
                     HDVIVMDRMLPGNIDGLGIIEALRKAGNHTPILILSALDAVDERIRGLRAGGDDYLTK
                     PFAFGELLARVDALGRRSSGGDAERTLQVADLRMDLLSRRVTRGTRAVTLQPREFKLL
                     EYLMRHANQVVTRTMLLEAVWEYNFDPQTNVVDVHISKLRQKIELDSERPLVRTVRNA
                     GYMLTDHE"
     gene            2854..4233
                     /locus_tag="VM713_04885"
     CDS             2854..4233
                     /locus_tag="VM713_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH83637.1"
                     /translation="MNRLIRSSPVTWALGYLLLGLAALVAFAVLLWYAWNVSIFSGRE
                     EILRAEAQHLTDVFAREGPDGLTAHIKARLRLQIVGERTLLLTDAAYRPLAGNLAAWP
                     REIPDDSGTHAVMVEHLAGQPTHTLLVRAALPGGYHLLVGRDLGFYLPLERQFAFGLT
                     AAVTFLFVVGVAGAILIRRQLLARVQGIRTTVSAIMAGDLRRRLPPAGSDDELDTLSQ
                     TINGMLDHIERLIHGISDVSNSIAHDLRTPLAELRSRLEELTLTRPEPAETFAEIEAA
                     VVDVDRVMRIFAALLRLAELDTGARRSGFVQVDASRVAAEVVEFYEPAAEQKGVAFSF
                     EARGAAPVAGDPVLLAQALSNLIDNSLKCVPERGAIRVCVDRRDDGSVAIEVADNGPG
                     IAAADKPKVTERFFRGDTSRGTAGVGLGLSIVDAVARLHGGVLELLDNHPGLRAQMVL
                     PVTDGRSSR"
     gene            complement(4208..>5753)
                     /locus_tag="VM713_04890"
     CDS             complement(4208..>5753)
                     /locus_tag="VM713_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83638.1"
                     /translation="AAQDTKAGADKAEPASDAGALMRLCHIDVKGDLKDEWKRLPVAQ
                     SSVTEHHFGGAGKSFDYTATAGTLIVRDDEDKPIASMGYVAYTKKGGKDGELRPVTFA
                     FNGGPGSSSLWLHMGVLGPKRVVVADPTPTPNAPYRTVENQYGLLDKSDVVMIDPVGT
                     GLSHAVCNHKDDEFFGVDPDIDSVSRLIAQYVSDNNRWTSPKYLLGESYGTTRGAAIV
                     NWLRVHRALEFNGLILVSVATDIEAIFAELPGNDRPYSVYLPGYAAVAWYQKALPHQP
                     PAFEPFLAEVRAYAMGPYTAALLKGDGLSDAERDAVAGQLHEYTGLSTEYLKFANLRV
                     SEIMFAHELLKNQRKTIGRLDGRFVGPTQDPLAKEADYDPQFAAIGAAFTAAFFDYYH
                     GELKFGQGKTYYTTNFGIGEKWKFTHKTDNGEQPLVNSGVDLAQALVQDQNLKVLVLN
                     GYFDLATPFSATEYMMSHLGVPASASARISMKYYPAGHMMYVNPQSIAKMKKDLDDFI
                     DSTDRL"
BASE COUNT          906 a         1999 c         1840 g         1008 t
ORIGIN      
        1 gaccaggtga tcgaccgtac catcaccatc caggcctcgg tgcgcgacgt cgagctctcg
       61 ctcctcatcg ccatcctcct cgtggtgacg gtgatcttcc tgttcctgcg cagcttctgg
      121 gcgaccgcga tcccgggcat caccgtgccg ctcgcgctct tcggcaccgc cgcgctcatg
      181 tacctggtcg gctacagcct cgacaacctg tcgctgatgg ccctcaccat cgccgtgggc
      241 ttcgtggtcg acgatgcgat cgtgatgctc gagaacatct accgccacat cgaggagggc
      301 ctcaccccct accaggcggc cgtgaagggc gcgggtgaga ttggtttcac tatcctctcg
      361 atcagcattt cgctgatagc ggtgttcatc ccgctgctgc tgatgagcgg catcatcggg
      421 cggctgttcc gcgaattcgc ggtcacggtg accatgacca tcgcggtctc ggcggtggtg
      481 gcgctcaccc tctcgccgat gctgggggcg ctctacctgc gcgacgagaa gcacgcgcgc
      541 cacggccgcg cctaccagac gctcgagcgc ggctttgaat ggctcctcga ccattacacc
      601 cgcggcctgg atttcgtact gcgccaccag ggcgccacgc tcacgagctt cctcatcacg
      661 gtcggcgtcg cggtggcgct ctacgccatc gtgccgaagg gcttcttccc gcagcaggac
      721 accggaatca tcgccggcct caccgacgcc ccgcaggatg tgtccttcaa ccagatgatg
      781 cgcctgcagc gcaatctcac cggcatcgtc gccagggacc cggcggtcgc gggctacgcc
      841 gccttcgtcg gcggcgggcg cccgctcaac aacggcttcg tcatcatcgg cctgaagccg
      901 cgcgatgagc gcgacgtgac cgccgaccag gtcatcgagc ggctgcgccg gcagatcaac
      961 tcggttcccg acggcacctt gttcctgcag gcgcaacagg acctgaacgt cggcggacgt
     1021 ctgacgcgca cccagtacca gtacacgctg caggatccga atctggaaga gctcaacacc
     1081 tgggcgccgc ggctgctcga taagctgaag aagctgccgc agctgcgcga cgtggcctcc
     1141 gaccagcagt cgagcagcac caagctcgcg ctcgtgatcg accgcgacca ggcggcgcgc
     1201 ttcggcatcc agccggcgct catcgacaac accctctacg atgccttcgg ccagcgccag
     1261 gtgacgcagt acttcacgca gctcaacagc tatcacgtgg tgctggaggt gacgccgtcg
     1321 ctcctcggcg acccggacac gctgaggaag atctacatca agtcaccact caccgggcag
     1381 caggtgccgc tttccacctt tactcgctac gacaccgggc acaccagcta cctgtcggtg
     1441 aaccaccagg ggcagttccc agccgtcact ctctccttca atctcgcgcc gggagtcgcc
     1501 ctcggcgatg cggtgacggc gatccagaag accgcggccg ccattcacct gccggcggcg
     1561 atcctcggca ccttccaggg cacggcgcag gccttcaagg actcgctcag caccgcgccg
     1621 ctgctgatcc tggcggcgct gctcgccgtg tacatcatcc tgggagtcct gtacgagagc
     1681 tacatccacc cgctcaccat tctctcgacg ctcccctcgg caggcgtcgg cgcgctcctc
     1741 atgctgatgt tgtttcactt cgacctgtcg gtcgtaggcc tcatcggcat catcctgctc
     1801 atcggcctcg tgaagaagaa cggcatcatg atggtggact tcgcgctgca cgccgagcgc
     1861 aatgaggggc tcgagccgca ggcggcgatc cgcaaggcct gcctgctgcg cttccgcccc
     1921 atcatgatga cgaccatggc ggcgatcctc ggcgcgctgc cgctcatgct cggccgcggc
     1981 acgggctcgg agctgcgcca gccgctcggc tttacaatgg tgggaggatt ggtgttgagc
     2041 cagatgctga ccctgttcac caccccggtc gtctatctgt acctggatcg cctgtcggtg
     2101 cgctgggcgc gcaaacatcc gcatcgcgac gtgggcggcg gcgccgtggc ggccgcggac
     2161 gccgcgcagg cctcagcgcc gtgaagattc tcctcgtcga ggacaacgag cgggtggcac
     2221 ggttcgtgac caagggcctg cgcgaggcgg gccacacagt cgagcacgcc ggcaacggcc
     2281 gcgacggtat gtttctcgct gccagcgagt cccacgacgt catcgtcatg gaccgcatgc
     2341 ttcccggcaa catcgacggg ctcggcatca tcgaggcgct gcgcaaggcg ggcaatcaca
     2401 ctccgatcct gatcctctcg gcgctcgatg cggtcgatga gcgcatcaga gggctgcgcg
     2461 cggggggcga cgactacctc accaagccct tcgccttcgg tgagctcctg gcacgggtcg
     2521 acgccctcgg ccggcgcagc tccgggggcg atgccgagcg gacactccag gtcgccgacc
     2581 tgcgcatgga cctgctgtcg cgacgggtga cccgcggcac ccgcgccgtg acgctgcagc
     2641 cgcgcgagtt caagctgctc gagtacctga tgcgtcacgc caaccaggtg gtgacccgca
     2701 ccatgctgct ggaagcggtg tgggaatata acttcgaccc tcagaccaac gtagtcgacg
     2761 tgcacatctc caagctccgc cagaagatcg agctcgactc ggagcggccg ctggtgcgta
     2821 ccgtgcgcaa cgccggctac atgctcaccg accatgaata ggctgatccg ttcctccccc
     2881 gtcacctggg cgctcggcta cctgctgctt gggctcgccg cactggtcgc cttcgcggtg
     2941 ctcctgtggt acgcgtggaa cgtgagcatc ttctccggcc gcgaggagat cctgcgcgcc
     3001 gaggcgcagc acctcacgga tgtgttcgcg cgcgaggggc ccgacggact cactgcgcac
     3061 atcaaggcgc gcctgcggct gcagatcgtc ggggagcgga cgctcctcct gaccgacgcc
     3121 gcctaccggc cgctcgccgg caacctcgct gcctggccgc gcgagattcc cgacgactcc
     3181 ggcacgcacg cggtgatggt ggagcacctg gccgggcagc caacccacac actgctggtg
     3241 cgcgcagctc tcccgggcgg ctatcacctg ctggtcggcc gcgacctcgg cttctacctg
     3301 ccgctcgagc ggcaattcgc cttcggcctc accgccgcgg tgaccttcct cttcgtggtg
     3361 ggcgtcgcgg gcgccattct catccgccgc cagctgctcg cgcgcgtgca gggcatccgc
     3421 acgacggtgt cggccatcat ggcgggcgat ctgcgccggc ggctaccgcc ggcgggctcg
     3481 gacgatgagc tcgacacgct atcgcagact atcaacggca tgctcgatca catcgagcgg
     3541 ctgattcacg gcatcagcga cgtctccaac tccatcgccc acgacctgcg taccccgctc
     3601 gccgagctgc gctcgcgcct cgaggagctc acgctcaccc gccccgagcc cgccgagacc
     3661 tttgccgaga tcgaggcggc ggtcgtcgac gtcgaccgcg tgatgcgcat cttcgccgcg
     3721 ctcctgcgtc tcgcggagct cgataccgga gcgcggcgct ccggcttcgt gcaggtggac
     3781 gcctcgaggg tcgccgcgga agtggtcgag ttctacgagc cggccgccga gcagaaaggc
     3841 gttgccttct cgttcgaggc gcggggcgcc gcgccggtgg ccggcgaccc ggtgctgctc
     3901 gcgcaggcct tgagcaacct cattgacaat tcgctcaagt gcgtgccgga gcgcggcgcc
     3961 atccgggtgt gcgtcgacag gcgggacgac ggctcggtcg cgatcgaggt ggccgacaac
     4021 ggccccggca tcgccgcggc cgacaagccg aaagtcaccg agcgcttctt ccgcggcgac
     4081 acgagccgcg ggacggcggg cgtggggctc ggactgtcca tcgtcgacgc cgtggcgagg
     4141 ctccacggtg gcgtgctcga gctgctcgac aaccatccgg gcctcagggc ccagatggtg
     4201 ctgccggtca cagacggtcg gtcgagtcga tgaagtcgtc gagatccttc ttcatcttgg
     4261 cgatcgactg cggattcaca tacatcatgt gcccggccgg gtagtacttc atcgagatac
     4321 gtgcgcttgc cgaggccggc acgccaaggt gggacatcat gtactcggtc gccgagaagg
     4381 gcgtggcgag gtcgaagtag ccgttcaaca ccagcacctt gaggttctgg tcctgcacga
     4441 gcgcctgggc gagatccact cctgagttga cgagcggctg ctcgccgttg tcggtcttgt
     4501 gcgtgaattt ccacttctcg ccgataccga aattcgtcgt gtagtaggtc ttgccctgtc
     4561 cgaacttgag ctcgccgtgg tagtagtcga agaacgccgc ggtgaaggcc gcaccgatgg
     4621 cggcgaactg cggatcgtaa tccgcctctt tggcgagtgg gtcctgcgtg ggtccgacga
     4681 agcggccatc gagccggccg atggtcttgc gctgattctt caggagctcg tgggcgaaca
     4741 tgatctccga gacccgcagg ttcgcgaact tgaggtactc ggtcgagagg ccggtgtact
     4801 cgtgcagctg gcccgcgacc gcatcgcgct cggcatcgct taagccatcg ccctttaaga
     4861 gcgccgccgt gtacgggccc atcgcgtagg cgcgcacttc ggcgaggaac ggctcgaacg
     4921 cgggcggctg gtgcggcagc gccttctgat accacgccac agcggcgtag cccggcagat
     4981 acaccgaata gggccggtcg ttgcccggga gctcggcgaa aatcgcctcg atatcggtcg
     5041 ccaccgagac caggatcaga ccgttgaact cgagcgcgcg atgcacgcgc agccagttga
     5101 caatcgccgc gccgcgcgtc gtgccatagc tctcgccgag caggtacttc ggcgaggtcc
     5161 agcggttgtt gtcgctcacg tattgcgcga tgaggcggct gaccgagtcg atgtcggggt
     5221 cgaccccgaa gaactcatcg tccttgtggt tgcagacggc gtgggagagc cccgtgccca
     5281 cgggatcgat catgacgaca tcgctcttgt cgagcagacc gtactggttc tcgacggtgc
     5341 ggtagggcgc gttcggggtc ggggtaggat ccgcgaccac cacgcgcttc ggtccgagca
     5401 ctcccatgtg cagccacaat gaagaggagc ccggcccgcc gttgaacgcg aaggtgacgg
     5461 gacgcagctc gccgtccttg ccacccttct tggtgtaggc cacgtacccc atgctcgcga
     5521 tcggcttgtc ctcgtcgtcg cgcacgatga gggtgcctgc ggtggcggtg tagtcaaagg
     5581 acttgcctgc gccgccgaag tgatgctcgg tgaccgagct ctgcgccacg ggcagcctct
     5641 tccactcgtc ctttaggtcc cccttgacgt cgatatgaca caggcgcatc agcgcgcccg
     5701 catcactcgc cggctccgcc ttgtcggcgc cggccttggt gtcctgcgcc gca
//
LOCUS       DATLKL010000374         2577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_196108, whole genome shotgun sequence.
ACCESSION   DATLKL010000374 DATLKL010000000
VERSION     DATLKL010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196108"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VM713_04895"
     CDS             <1..696
                     /locus_tag="VM713_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HVH83639.1"
                     /translation="RLAGEACLRVGAGLTTVAVAPENVAAIAAGRPELICLALTEPRV
                     LADAAARADVVAIGPGLGTGPWARAALDSVLSSDRPLVVDADALNLLAEAEARPRGDW
                     ILTPHPGEAARLLDLTTLDIQHDRLAALDRLLARFGGTIVLKGAGTLVGSAGRTPGVC
                     ERGNPGMASAGTGDVLTGAIAGILAQCRDPWTAARVGVLVHAMAGDAAARGGERGVLA
                     SDLARELHHAVNL"
     gene            707..1186
                     /gene="tsaE"
                     /locus_tag="VM713_04900"
     CDS             707..1186
                     /gene="tsaE"
                     /locus_tag="VM713_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HVH83640.1"
                     /translation="MRTRTLTAEETEELGARVARMRPAGDAALTVLYLRGELGSGKTT
                     FARGFARAAGVRGLVRSPTYTLLELYPAGSVTLVHLDLYRVQSPEELGSLGLREWATP
                     HHVWLVEWPEHGAGRLPAADVTVAFAAGTAGHDVELAATSALGAAWLVQLESHAGTP"
     gene            1216..2454
                     /locus_tag="VM713_04905"
     CDS             1216..2454
                     /locus_tag="VM713_04905"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVH83641.1"
                     /translation="MIRKVFLRGLIGSLLTLPVLSTATAAELRSMQLSATADNAVMTL
                     ALTDGPAAKVFTLEGPGRAVIDLPRTHARHGMVVPAPAGIVSAVRLGKQPGDTLRIVL
                     ELKSAVAVHSSLAAGASGGRQLTVALGESAAAAVDAGPKVVPAPHAPGDGDRDIIIAV
                     DAGHGGVDPGAIGHGGTREKDVTLAIARDLAARIDTEPGMRAVLTRDRDEFLELRERI
                     QRARAARADMFVSIHADSIADRSVSGASVYILSVHGASSEAAKELAEGQNAADLMGGV
                     HLDEVGAAAPVVLDLVQQEAIGLSATAAERVVSALERVGEVRRAQVQRAGFVVLKSPY
                     IPSMLIETAYISNPAEERRLRNPDQQVRLADAICGGVRSYFLQHPPDGTRFKQERRVA
                     SAAEVAAAVTGLTAATPLNP"
BASE COUNT          355 a          913 c          913 g          396 t
ORIGIN      
        1 cgccttgcgg gggaggcctg cctgcgcgtc ggtgccgggc tcacgacggt ggctgtggcg
       61 cccgagaacg tcgccgcgat cgccgccggc cgacccgagc tcatctgcct cgcgctcacc
      121 gagccgcggg tgctggcgga cgcggccgcg cgcgcggacg tggtcgcaat agggccgggc
      181 ctcggaaccg gtccttgggc ccgtgcggcg ctcgattcgg tgctctcctc tgacaggcca
      241 ctggtggtcg acgcggacgc gctcaacctg ttggccgagg ccgaagcgcg cccgcgcggc
      301 gactggatcc ttacgccgca cccgggcgag gctgcacggc tgctcgacct cacgacactg
      361 gatatccagc acgatcggct cgcggcactc gatcggctgc tggcgcgctt cggcggaacc
      421 atcgtgttga agggcgccgg caccctggtc ggctccgcgg gccggacgcc gggcgtgtgc
      481 gagcggggca atcccggcat ggcgagcgcc ggcaccggcg atgtgctcac gggggcgatc
      541 gccggcatcc tcgcgcagtg ccgcgacccc tggaccgctg cgcgcgtcgg cgtgctggta
      601 cacgccatgg caggcgacgc cgccgctcgc ggcggcgagc gcggtgtgct cgcgagcgat
      661 ctggcgcggg agctgcacca cgcagtcaac ctctagagcc gctgcggtgc ggacgcggac
      721 cctgacggcc gaggagaccg aagagctcgg cgcgcgggtc gcccgcatgc ggcccgcagg
      781 cgatgcggcc ctgacagtgt tgtacctgcg cggcgagctc ggctccggca agaccacgtt
      841 cgcgcgcggg tttgcccgcg cggcaggtgt gcgcgggttg gtgcgcagcc cgacttacac
      901 gctgctcgag ctgtaccccg ccggcagcgt taccctcgtg cacctggacc tctaccgggt
      961 gcagtcgccc gaagagcttg gctccctggg attgcgcgag tgggcaacgc ctcatcacgt
     1021 gtggctcgtc gaatggcctg agcatggcgc cgggcgcctc cccgcagcgg acgtcaccgt
     1081 ggctttcgcc gctggcacgg ccggccacga cgtcgagctc gccgcgactt cggcactcgg
     1141 cgctgcctgg ctggtccaac ttgaaagtca tgcaggaacg ccgtagatta cgtgggagct
     1201 tacgccgcga acttgatgat tagaaaggta tttcttcgag gactcatcgg cagtctgctg
     1261 acgctgcctg tcctctcgac ggcgaccgca gcggagctgc gctccatgca gttgtctgcg
     1321 acagccgaca acgccgtcat gaccctcgcg ttgaccgacg gcccggcggc caaggttttc
     1381 acgcttgagg gccccggccg cgcggtcatc gatcttcccc gcacgcacgc caggcacggc
     1441 atggtggtac ccgcgccggc cggtatcgtg agcgccgtgc ggttaggtaa gcagccgggc
     1501 gacacgctgc gcatcgtcct cgagctcaag tcggcagtcg ctgtgcacag ctctctggcc
     1561 gcgggagcgt cgggcggccg gcagctgacc gtggcgctcg gtgagtccgc ggcggcggct
     1621 gtggacgcgg gacccaaggt ggtccccgcg ccgcacgcgc ccggcgatgg cgaccgtgac
     1681 atcatcatcg cggtggatgc gggccatggc ggcgtcgatc ccggcgccat cggacacggt
     1741 ggcactcgcg agaaggacgt cacgctcgcg atcgcccggg acctcgccgc gcgcatcgac
     1801 accgagcccg ggatgcgcgc cgtgctgacg cgcgatcggg acgagtttct cgagctgcgc
     1861 gagcgcatcc agcgtgcccg cgcggcacgc gccgacatgt tcgtgtccat acatgcggac
     1921 tcgatcgccg atcgcagcgt ctcgggcgct tcggtgtaca tcctgtcggt gcacggggcc
     1981 tcgagcgagg cggccaagga gctggccgag ggacagaacg cggcggatct catgggcggc
     2041 gtgcacctgg atgaggtcgg cgcagctgct cccgtggtcc tcgatctggt gcagcaggaa
     2101 gccatcggcc tgagcgcgac ggcggccgag cgcgtggtat ccgctctcga gcgcgtcggc
     2161 gaagtgcgca gggcccaggt gcagcgcgcc ggcttcgtag tgctcaagtc cccctacatt
     2221 ccctcgatgc tcatcgagac cgcctacatc tccaaccccg ccgaagagcg ccgcctgcgt
     2281 aatcccgatc aacaggtacg tctcgcggat gcgatctgcg gcggtgtacg cagctacttc
     2341 ctgcagcatc cgcccgacgg cacgcgcttc aagcaggagc gccgggtcgc cagcgctgcg
     2401 gaggtggcgg ctgcggtcac cgggctcacc gccgcgacgc ccctcaaccc ataagcctcg
     2461 agtgcccgcc gtccgccgcc gcgaggcagg atcgcctgta cactgcgctg cgcgctcgat
     2521 gtgtgcgctg ccaatcgccc ttcagccgcc atgcccattc gcgttcttcc cagcgag
//
LOCUS       DATLKL010000375         2404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_49398, whole genome shotgun sequence.
ACCESSION   DATLKL010000375 DATLKL010000000
VERSION     DATLKL010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49398"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VM713_04910"
     CDS             <1..1372
                     /locus_tag="VM713_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVH83642.1"
                     /translation="VVIVGSSIGDNRAADVERGVIRGFDALSGALIWSFDPLPDSPAH
                     PAAAEWNLAQAAGTGAGNAWGVMTVDEEHALVLVPTGSASPDFYGGTRSGSNRFADSL
                     LALDARTGKLTWHQQLVHHDLWDYDLAAQPALGDVDVRGVPVAAVIEATKTGMLYVFD
                     RTDGAPVFPVSERPVPPSLVPGEQAWPTQPFSALPALVPQRPVNPADAWGLTFWDRGK
                     CREKLAALRNEGIFTPPDTRGTLEFPGYAGGVNWGGVVFDEQRGRVIAAVNDLPMVVT
                     LIPSASLEAEQRSGLHPQAEFGRQAGTPYAMRREPLLSPWGLPCTAPPWGTLVNVDLH
                     ANRIVWQVPLGSTEGFTPWPLVRDFGMPGMGGPIATAGELVFVGAAMDSYFRAFDIET
                     GRELWKHKLPAGGQATPMTYRAGQDERQYIVISAGGHGALGTPRGDYVIAFALPPAAR
                     AARAAR"
     gene            1390..2130
                     /locus_tag="VM713_04915"
     CDS             1390..2130
                     /locus_tag="VM713_04915"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH83643.1"
                     /translation="MSLLFIVSLLIQAGFIIHVIKTGRNQLWIWVLIIPGLAWAGIVA
                     YIVVEILPELLRGRTARRTARGFRRALDPGAELRRYENEASVTGNVASRQRYAEELTR
                     HERYDEAIAQYHQALTGLYEHDPNLMLGLAQAQFGKGDARAARTTLDELIRQNPGFRS
                     PEGHLLYARSLAAEGNVAKALEEYRVLAPSYPGAEAAARYAQLLEAEGQHAEAQRVAR
                     ELIEQARIAPGHYRRAQREWLEAAQRLL"
     gene            complement(2141..>2404)
                     /locus_tag="VM713_04920"
     CDS             complement(2141..>2404)
                     /locus_tag="VM713_04920"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HVH83644.1"
                     /translation="RVAGERLAASYVNFYIANGGIVMPLLDERTDRRAAAAQKRLFPR
                     RRVVGVPAREILLGGGNIHCITQQIPLGRGAGRQSRASSASAR"
BASE COUNT          354 a          838 c          849 g          363 t
ORIGIN      
        1 cgtggtgatc gtcggctcct cgattggcga caatcgcgcc gcggacgtcg agcgtggcgt
       61 gatccgcggc ttcgatgcgc tgagcggcgc cctcatctgg tcgttcgatc cgctgccgga
      121 ctcgccggca catcccgccg ccgccgaatg gaacctcgca caggccgcgg gcaccggtgc
      181 cggcaatgcc tggggcgtca tgacagtgga cgaggagcac gcgctggtgc tggtgccgac
      241 cggctcggcc agtcccgact tctacggcgg cacgcgctca gggagcaatc gcttcgccga
      301 ctcgttgctg gccctcgatg cgcgcaccgg taagctcacg tggcaccagc agctggtgca
      361 ccacgacctg tgggactacg acctcgcggc gcaaccggcg ctcggggatg tcgacgtgcg
      421 cggcgtaccg gtggcggccg tgatcgaggc gaccaagacc ggcatgctgt acgtgttcga
      481 tcgcaccgac ggcgcgccgg tcttcccggt gagcgagcga ccggtaccgc cgagcctcgt
      541 gccgggcgag caggcctggc cgacccagcc gttctcggcg ctgccggcgc tcgtcccgca
      601 gcggcccgtc aatcccgcag acgcctgggg cctcaccttc tgggaccggg gcaagtgtcg
      661 cgagaagctc gcagcactgc gcaacgaggg catcttcacc ccgcccgaca cgcgcggcac
      721 gctcgagttt cccggttacg ccggcggtgt gaactggggt ggcgtggtct tcgacgagca
      781 acgcgggcgc gtgatcgccg cagtgaatga tctgcctatg gtggtgacgc tgatcccgag
      841 cgcgagcctc gaagccgagc agcgctcggg gctgcacccg caggccgagt tcggacgcca
      901 ggcgggtacc ccctatgcaa tgcgccgcga gccgctgctc tcgccgtggg gcctgccgtg
      961 caccgcccct ccctggggta cgctggtgaa cgtggatctg cacgccaacc gcatcgtctg
     1021 gcaggtgccg cttgggtcga ccgagggatt cacgccgtgg ccgctggtgc gcgacttcgg
     1081 catgccgggc atgggcggac ccatcgccac ggcgggcgag ctggtgttcg tcggcgctgc
     1141 gatggacagc tacttccgcg ccttcgacat cgagaccggg cgcgagttgt ggaagcacaa
     1201 gctccccgcc ggcggccagg ccacgccgat gacctaccgc gccggacagg atgagcggca
     1261 gtacatcgtc atcagcgccg gcggccacgg tgcgctcggc actccgcgcg gagactacgt
     1321 cattgcgttt gcgctgccgc cagcagcgcg cgcggcgcgc gcggcgcgct agggccgcgg
     1381 agagaccgga tgtcgctact gttcatcgtt tcgttgctga tccaggcggg gttcatcatt
     1441 cacgtcatca agaccgggcg taatcagctg tggatctggg tcctcatcat ccccggactg
     1501 gcctgggccg gcatcgtcgc ctacatcgtc gtcgagatcc tcccggagct gctgcgcggg
     1561 cgtaccgcac ggcgtaccgc gcgcggattc aggcgtgcgc tggatccggg cgccgagctg
     1621 cgccgctatg agaacgaggc aagcgtcacc ggcaatgtgg cgagccgtca gcgctacgcc
     1681 gaggagctca cgcgccacga gcgctatgac gaggcgatcg cccagtacca ccaggcgctc
     1741 accgggctct atgagcatga cccgaatctc atgctgggcc tcgcccaggc gcagttcggc
     1801 aagggcgacg cgcgcgctgc gcgcacgacg ctcgatgagc tcatccggca aaaccccggc
     1861 tttcgctctc cggaaggcca tctgctctac gcccgctcgc tcgcggccga gggcaacgta
     1921 gccaaggcgc tggaggagta ccgcgtgctc gcgccgtcct acccgggtgc cgaggccgca
     1981 gcgcgctacg cacagctgct cgaggccgag ggtcagcacg ccgaggcgca gcgcgtggcg
     2041 cgcgagctca tcgagcaggc gcgcatcgca ccggggcact accgccgcgc gcagcgcgag
     2101 tggctcgagg cggcgcagag gctactgtag cgcgggaact tcagcgcgcg ctggcgctcg
     2161 aggcgcgact ctggcgacct gcgcctctgc cgagcgggat ctgttgagtg atgcagtgaa
     2221 tgttgccgcc gccgaggagg atctcgcgcg ccggcacgcc gaccacgcgc cggcgcggaa
     2281 acaggcgctt ctgtgctgcc gcggctcggc ggtcggtgcg ctcatcgagc agcggcatga
     2341 cgatcccgcc gttggcgata tagaagttga cgtagctcgc ggccagccgc tcacccgcga
     2401 cgcg
//
LOCUS       DATLKL010000376         3595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_173509, whole genome shotgun sequence.
ACCESSION   DATLKL010000376 DATLKL010000000
VERSION     DATLKL010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173509"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VM713_04925"
     CDS             complement(<1..1003)
                     /locus_tag="VM713_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH83645.1"
                     /translation="MFELPPVFLALAFVLVGLATYFLGRRNGAARTVRLPRDYYVGLD
                     HLINDRFDHAAEIFARMAEADGDAAEIQFALGSLFRRRGEVERAIAIHSRLREHGGGS
                     VSDKAAFALALDYLSAGLMDRAEQVLEELAASREYRNAALDHLVRIYESQGDWAKALK
                     VFHALPANVQLERRRVAAHYLCELAEHALLQGDPERACTLLRQARTHQSELPRADILT
                     ARIAEGEGDADGALLLYLQALEMAPGLALEIIPAVLRIAGAAGGLDVMQELGRRLRRS
                     GRVTPRQLAWLLATAVPAGDVARLPGIAAELGPGVQSVADATSLSALLTRIGDAGGRY
                     "
     gene            complement(1074..1376)
                     /locus_tag="VM713_04930"
     CDS             complement(1074..1376)
                     /locus_tag="VM713_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HVH83646.1"
                     /translation="MTKSELIEIITAKQKHLPAKDVELALKQILEIMSDALSQGERIE
                     IRGFGSFSLHFRPPRQGRNPKTGEAVALSGKHVPHFKPGKDLRERVNDGADRPIRD"
     gene            complement(1383..3068)
                     /gene="rpsA"
                     /locus_tag="VM713_04935"
     CDS             complement(1383..3068)
                     /gene="rpsA"
                     /locus_tag="VM713_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016568693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HVH83647.1"
                     /translation="MSESFAQLFEQSLANQRIRPGMILTGLVVDVTPDVVIVNVGLKS
                     EAVIPLEQFKNERGEVEVKPGDQVEVALDSVEDGTGETRLSREKAKRARTWTRLEEAF
                     NKSEIVTGVITGRVKGGFTVEIENVRAFLPGSLVDVRPVRDTSYLEGKPLEFKVIKLD
                     QKRNNVVVSRRAVVEQEFSAERSALLENLQEGAVVRGAVKNLTDYGACVDLGGIDGLL
                     HITDMAWKRVKHPSEVVKVGDEVEVRILKFDRERSRVSLGLKQLGADPWENIARRYPP
                     GTRVFGKVTNIADYGAFVEIEDGVEGLVHVSEMDWTNKNVNPAKVVHSGQEVEVMVLD
                     VDEERRRISLGLKQCQANPWKEFAENYNRGDKVAGQIKSITDFGIFIGLPGNIDGLVH
                     LSDISWDQPGEEAVRNYQKGQQLEAMVLSIDPERERISLGIKQLAKDPFSAYIADNPK
                     GTIVKGVVKEVDARGAVIDLGNGIEGQLRASELGRDRVEDARAVLKVGDEIEAKFTGV
                     DRKSRTISLSIKAKEAHDEAEAVQSYRSEVAPSGTSLGDLLKEHIGSGGERGS"
     gene            complement(3205..>3595)
                     /gene="cmk"
                     /locus_tag="VM713_04940"
     CDS             complement(3205..>3595)
                     /gene="cmk"
                     /locus_tag="VM713_04940"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HVH83648.1"
                     /translation="GASRVAAWAQVRGALLERQRGFAEPPGLVADGRDMGTVVFPQAD
                     LKIFLTATPEERALRRYKQLKDKGSDVSLAALSREIAERDLRDRTRQVAPLRPAADAL
                     VIDSTGLDIGAVVARVLALGVARRLWP"
BASE COUNT          567 a         1267 c         1169 g          592 t
ORIGIN      
        1 gatagcgccc gccggcatcc ccgatgcgcg tcagcagcgc actcagcgag gtcgcatccg
       61 cgaccgactg cacgccgggt ccgagctccg cggcgattcc gggaagtcgt gccacgtcgc
      121 cggccggaac cgccgtcgcg agcagccagg cgagctggcg cggcgtgacg cggccgctgc
      181 gcctgaggcg gcggccgagc tcctgcatga cgtcgaggcc gcccgcggcg cccgcgatgc
      241 gcagcaccgc cggaatgatc tcgagcgcga gcccgggcgc catctccagc gcctgcagat
      301 aaagcagcag cgcgccgtcg gcgtcgccct caccctcggc aatgcgcgcg gtgaggatat
      361 cggcccgcgg aagctcgctc tggtgggtgc gcgcctggcg cagcagggta cacgcccgct
      421 ccgggtcgcc ctgcagcagg gcgtgctcgg cgagctcgca caggtagtgc gccgcgaccc
      481 gccggcgctc gagctgcacg ttcgccggca gcgcatgaaa gaccttgagc gccttggccc
      541 agtccccctg ggattcgtag atgcgcacca ggtgatcgag cgcggcgttg cggtactcgc
      601 gtgaggcggc cagctcctcc agtacctgct cggcgcggtc catgaggccg gcgctcaggt
      661 aatcgagggc cagggcaaag gccgccttgt cgctgacgct gccgcccccg tgctcgcgca
      721 ggcgcgagtg aatcgcgatg gcgcgctcga cctcgccgcg ccggcggaac aggctcccga
      781 gggcaaactg gatctcggcg gcatcgccgt ccgcctccgc catgcgcgcg aagatttccg
      841 cggcgtggtc gaagcggtcg ttgatcaggt gatcgaggcc gacgtagtag tcgcgcggca
      901 gccgcacggt acgcgcggcg ccgttgcggc gcccgaggaa gtaggtggcg agcccgacca
      961 ggacgaaggc cagcgccaga aagaccggag gcagctcaaa cacagcgctg acgataacag
     1021 gtccgctgcg cgagcgataa cggccgccag caggcggccg cccgagcact cgctcagtcg
     1081 cggatcgggc ggtcagcgcc gtcgttgacg cgctcgcgca ggtccttgcc gggcttgaag
     1141 tgcggcacgt gcttgcccga aagcgccacc gcctcgccag tcttgggatt gcgcccctgg
     1201 cggggcgggc ggaaatgcag cgagaagctg ccgaagcccc ggatctcgat gcgctccccc
     1261 tgcgagagcg catcgctcat gatctcgagg atctgcttca gagccagctc gacgtccttg
     1321 gccggcaggt gcttctgctt ggcggtgatg atctcgatga gctcggactt cgtcatggcg
     1381 cttcagctgc cgcgctcgcc gcccgagccg atgtgctcct tgagcaggtc gccgagactg
     1441 gtgccgctcg gcgcgacttc cgagcggtag ctctgcaccg cctcggcctc gtcgtgcgcc
     1501 tccttcgcct tgatcgacag cgagatggtg cgcgacttgc ggtcgacgcc ggtgaacttg
     1561 gcctcgatct catccccgac cttgagcacc gcgcgcgcgt cctcgacgcg atcgcggccg
     1621 agctcggagg cgcgcagctg tccttcgatg ccgttgccga ggtcgatgac cgcgccgcgg
     1681 gcatcgactt ccttcaccac acccttgacg atcgtaccct tggggttatc ggcgatgtag
     1741 gcggagaacg gatccttggc gagctgcttg atgccgagcg agatgcgctc gcgctccgga
     1801 tcgatcgaca acaccatggc ctcgagctgc tggcccttct ggtagttgcg caccgcctcc
     1861 tcgcccggct gatcccagga gatgtccgag aggtgcacca ggccgtcgat gttgcccggg
     1921 agcccaatga agataccgaa gtcggtgatc gacttgatct gcccggccac cttgtcgccg
     1981 cgattgtagt tctcggcgaa ctccttccac gggttggcct ggcactgctt caagcccagc
     2041 gagatgcggc ggcgctcctc gtcgacgtcc agcaccatga cttcgacttc ctggccgctg
     2101 tgcaccacct tcgcgggatt gacgttcttg ttggtccagt ccatctccga gacgtgcacc
     2161 aggccctcga cgccgtcctc gatctctacg aacgcgccgt agtcagcgat gttggtgacc
     2221 ttgccgaaca cccgggtgcc cggcggatag cgccgcgcga tgttctccca gggatcggcg
     2281 ccgagttgct tcagtcccag gctgacgcgc gagcgctcgc ggtcgaactt gaggatccgc
     2341 acctcgacct catcgcccac cttgacgacc tcggaaggat gcttcacgcg cttccacgcc
     2401 atatcggtga tgtgcagcaa cccgtcgatg cccccgagat cgacgcacgc gccgtagtcc
     2461 gtgaggttct tgaccgcgcc gcgcaccacc gcgccctcct gcaggttctc gaggagcgcg
     2521 gagcgctcgg ccgagaactc ctgctcgacc actgcgcggc gggacaccac cacgttattg
     2581 cgcttctgat cgagcttgat caccttgaac tcgagcggct tgccttcgag ataagaggtg
     2641 tcgcgcaccg ggcgcacatc gacgagcgag cccggcagga acgcccggac gttctcgatc
     2701 tccacggtga agccgccctt gacgcggccc gtgatgacgc cggtgacgat ctcggacttg
     2761 ttgaacgcct cctcgagccg cgtccaggtg cgggcgcgct tggccttctc ccgcgagaga
     2821 cgcgtctcgc cagtgccgtc ctcgaccgag tcgagggcga cctccacctg gtcgccgggc
     2881 ttcacctcga cctcaccgcg ctcgttcttg aactgctcga gcggaatgac cgcttcggac
     2941 ttgaggccga cgttgacgat gacgacgtcg ggggtaacgt ctacgacgag cccggtcagg
     3001 atcatgccgg ggcggatgcg ctggttcgct agactttgct cgaagagctg ggcaaaactt
     3061 tctgacatac cttcactttg cacgccctgc gtgccgactc tggaccccgt ccctgggcgc
     3121 ccaggaaaaa gtaacaggcg cccgcatccc gcaggcacca acctcacata accttcctca
     3181 ccgcgtgact gaaagcgggc ggcctcaggg ccacaatcga cgggcgaccc ccagcgccag
     3241 cacccttgca acgaccgccc cgatgtcgag acccgtcgag tcgatcacca aggcatcggc
     3301 tgccggacgc aacggggcga cctggcgggt tctatccctc aagtcccgct cggctatctc
     3361 gcgcgaaagg gcggcaaggc taacatcgga ccccttgtct ttcaactgct tatagcggcg
     3421 aagagcccgc tcctccgggg tcgccgtgag gaatatcttg aggtcggcct gggggaagac
     3481 cacggtgccc atatcccggc cgtccgcgac gagcccgggc ggctcggcaa agcctctctg
     3541 gcgctcgagg agcgcaccgc gcacctgcgc ccacgccgcc acccgggaag ccccg
//
LOCUS       DATLKL010000377         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_117275, whole genome shotgun sequence.
ACCESSION   DATLKL010000377 DATLKL010000000
VERSION     DATLKL010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117275"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VM713_04945"
     CDS             <1..761
                     /locus_tag="VM713_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HVH83649.1"
                     /translation="VISDEVGLSLEKATLNDLGDAKKIVVEKENTTIIDGAGKPADIK
                     ARIESIRQRIEEATSDYDKEKLQERVAKLSGGVAVIKVGAATEIEMKEKKARVEDALH
                     ATRAAVEEGVVPGGGVALIRVQRAIEKLEGANEDQTVGVRILSRSIEEPLRQIVENAG
                     EDAAVILNQVKQPPGQGNKSTSAYGYNAAKGEFGDLLEAGILDPTKVTRLALQNAASV
                     AGLLLTTEVMVAEAPKDEEHSHAGPPGGMGGMDM"
     gene            951..1886
                     /locus_tag="VM713_04950"
     CDS             951..1886
                     /locus_tag="VM713_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83650.1"
                     /translation="MPDPKARALVAALSLTWVALAACSRQGQDEMSWARAALERNDRL
                     EVIAADPQSRSFTVRSKDTRELRVVRADQLIAVPALPAAAAVTATAPSAAPAAAAAAG
                     TAANAAPDPAAPAATGTIAAPGEYATTPTPAQSPVFASGALQSDGAARRLPGQVLKSG
                     PGYTIEAGSAPVRGPVREAGPIVADAALAAAPIREAGGGGAVVELRHEPIVCQGARLL
                     QLDSLNLQFEGDAVSAQDGCEIHITNSRIRATGVGVSARAANVHIDNSLIEGDAASID
                     ASEGAQIYTATSRFRGMSRRLDTATVHDLGGNVWN"
     gene            1873..>2057
                     /locus_tag="VM713_04955"
     CDS             1873..>2057
                     /locus_tag="VM713_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83651.1"
                     /translation="MSGTDARARAALASAPAEVRSSLPRVLAASDFVAESCTRDPGLL
                     AALIERGDLTRPLTSTEL"
BASE COUNT          345 a          720 c          703 g          289 t
ORIGIN      
        1 cggtgatctc cgatgaggtc gggctgtcgc tcgagaaggc gacgctgaac gacctcggcg
       61 atgccaagaa gatcgtcgtg gagaaggaga acaccacgat catcgacggc gccggcaagc
      121 ccgccgacat caaggcgcgc atcgagtcga tccgccagcg gatcgaggag gccacctccg
      181 actatgacaa ggagaagctg caggagcgtg tcgcgaagct ctccggcggc gtggccgtga
      241 tcaaggtcgg agccgccacc gagatcgaga tgaaggagaa gaaggcccgc gtcgaggacg
      301 cgctgcacgc cacgcgtgcg gcggtcgagg agggcgtggt gccgggcggc ggcgtggcgc
      361 tgatccgcgt gcagagggcc atcgagaagc tcgagggcgc gaacgaggac cagacggtcg
      421 gcgtgcgcat cctgtcgcgc tcgatcgagg agccgctgcg ccagatcgtc gagaacgccg
      481 gcgaagatgc cgccgtgatc ctcaaccagg tcaagcagcc tccgggccag ggcaacaagt
      541 cgacgagcgc ctacggctat aacgccgcca agggcgagtt cggcgacctg ctcgaggcgg
      601 gcatcctgga tcccaccaag gtgacgcgtc ttgcactgca gaacgcggct tccgtggcag
      661 gtctgctgct caccaccgag gtcatggtgg ccgaggctcc gaaggacgag gagcacagcc
      721 acgcgggacc gccgggcggc atgggcggaa tggacatgta ggggaaccct ctctaaagag
      781 gtatcgattc tgtaacactg agccgttagc ctttacataa cggttagggg ttcgcgccag
      841 aggctctcag cctcaggcgc cgccccaaag acgcggaaca agacccccta ttccgcgcat
      901 cattcgcagg ccccctcgtg gggcctgttt ttttggttta tcgtttcccc atgcctgacc
      961 cgaaagcccg cgcactcgtc gccgcgctct ccctgacctg ggtggcgctc gctgcctgca
     1021 gccggcaggg ccaggacgag atgagctggg cccgcgcggc cctcgagcga aacgaccgcc
     1081 tcgaggtcat cgccgctgac ccgcagagcc gctccttcac cgtgcgcagc aaggacaccc
     1141 gtgagctgcg cgtcgtgcgc gcggatcaat tgatcgccgt gcctgcgctc ccggccgccg
     1201 ccgccgtcac cgcgaccgcc cccagcgcag ctcccgctgc cgccgccgcc gccggcactg
     1261 cggcgaacgc cgctccggat ccggccgctc cggctgcgac cggcacgata gctgcaccgg
     1321 gcgagtacgc aacgaccccg accccggcgc agtcgcccgt gtttgcatcg ggagcattgc
     1381 aatccgatgg tgccgcgcgc cgccttccgg gccaggtcct caagtccggc ccgggctaca
     1441 cgatcgaggc cggctccgcg ccggtccgcg gcccggtgcg tgaggcggga ccgatcgtgg
     1501 ccgacgccgc gctcgccgcc gccccgatac gcgaggcggg agggggcggc gccgtggtcg
     1561 agttgcgtca cgagccgatc gtctgccagg gggcgcggct gctgcaactc gacagcctca
     1621 atctccagtt cgaaggcgat gccgtgagcg cccaggacgg ctgcgagatt cacatcacca
     1681 acagccgcat ccgggcgacg ggcgtcggag tgtcggcgcg cgccgccaac gtccacatcg
     1741 acaacagcct gatcgagggc gatgccgcct cgatcgatgc ctcggagggg gcgcagatct
     1801 atactgcgac ctcgagattc cgggggatgt cccgccggct cgacaccgca accgtgcacg
     1861 acctcggggg caatgtctgg aactgacgcg cgcgcgcgcg cggccctcgc gtcggctccc
     1921 gccgaagttc gcagctcgct cccgcgggtg ctggcggcga gcgatttcgt cgccgagtcg
     1981 tgcacgcgcg atccaggtct cctcgccgcg ctcatcgagc gcggcgatct cacccggcct
     2041 ctgacctcca ccgagct
//
LOCUS       DATLKL010000378         1582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_60815, whole genome shotgun sequence.
ACCESSION   DATLKL010000378 DATLKL010000000
VERSION     DATLKL010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60815"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1582
                     /locus_tag="VM713_04960"
     CDS             <1..>1582
                     /locus_tag="VM713_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH83652.1"
                     /translation="MAITRNISRRKALEQELRRARRRADDANAAKSAFLARMSHELRT
                     PMNGVLGMTSLLLDTRLDAAQRRFAEGIHRSADSLLAIVDDVLDLSKIEAGRLQLDPT
                     DCDLMELIEQTAEVLAPRASGKGIELLVDMPPEPLPHVRVDATRLRQVLINLGGNAVK
                     FTERGEVTLRAVALGVIPGTGAADAAADGKITGSALRVRLEVADTGIGIAPENQAKIF
                     EEFAQEDASTTRRFGGTGLGLAISRQIVELMGSELNLVSAPGIGSTFSCELTLAVSAA
                     QPPRSAPRPRLDAIRVLVADDNAAARVLTANALRAWGARPTLAESLEAALAELRGGAY
                     EAVIIDDPLPDGDARQLLRRLRGLGAARPRVIRLVGFASLKPVAAEGERDEWFDAEVT
                     KPLRLAQLHAALTGRATASERETQGACRRTPPQQLSGRVLVVEDQPLNREVAHGMLSS
                     LGITVDTAADGAQALERLQLESFDLVLMDCEMPVMDGFSATRELRRGEAAERRLPVIA
                     LTADVTREGRSACLAAGMD"
BASE COUNT          238 a          559 c          569 g          216 t
ORIGIN      
        1 gatggcgatc acccgcaaca tctcgcgccg caaggcgctg gagcaggagc tgcggcgggc
       61 ccggcgccgg gccgacgacg ccaacgccgc caagagcgcg ttcctggccc gcatgagcca
      121 cgagctgcgc acgccgatga acggcgtcct cggcatgacc agcctgctgc tcgacacccg
      181 cctggatgcg gcccagcggc gcttcgcgga aggtatacat cgctccgccg attcactgct
      241 cgccatcgtc gacgacgtgc tcgacctgtc caagatcgaa gcggggcggc tgcagctcga
      301 tcccaccgac tgtgatctga tggagctcat cgagcagacg gcggaagtgt tggcgccgcg
      361 cgcgagtggc aagggcatcg agctcctggt cgacatgcct cccgagccgc tgccgcacgt
      421 gcgggtcgac gccactcggc ttcgccaggt gttgatcaac ctcggaggca acgccgtcaa
      481 gttcaccgag cgcggcgagg tcaccctgcg cgccgtggcc ctgggggtga tcccggggac
      541 gggcgctgct gacgccgcgg ccgacggcaa gataacgggc agcgcgctgc gggtgcgact
      601 cgaggtcgcc gacacgggta tcggcatcgc tcccgagaac caggcgaaga tcttcgagga
      661 gttcgcccag gaggacgcgt cgaccacgcg gcgcttcggc ggcacgggcc tggggctggc
      721 gatctccagg cagatcgtcg agctgatggg cagcgagctc aacctggtaa gcgcccccgg
      781 gatcggctcg acgttctcct gcgagctcac cctggcagtg agcgctgcgc aacctccgcg
      841 tagcgccccg cgcccgcgcc tcgatgccat acgcgttctg gtggcggacg acaacgccgc
      901 cgcgcgcgtc ctcacggcca acgcgctgcg cgcctggggc gcgcgcccga cgcttgccga
      961 atcgctcgag gccgcgctcg ccgagctgcg cggcggggcg tacgaggcgg taatcatcga
     1021 cgatccgctc ccggacggcg acgcccgcca gctcctgcgg cggctgcggg gcctcggcgc
     1081 cgcgcgcccg cgagtgattc gcctggtggg cttcgcgagc ctgaagccgg tggcggcgga
     1141 gggcgagcgc gacgagtggt tcgatgccga agtgaccaaa ccgttgcgcc tcgcgcagct
     1201 gcacgccgca ctcaccgggc gtgcgaccgc gagcgagcgc gagacccagg gggcctgccg
     1261 gcgcaccccg ccgcagcagc tctcgggcag ggtgctggtg gtcgaggatc agcccctcaa
     1321 ccgcgaagtt gctcacggca tgctgagctc gctgggcatc acggtggata ccgccgccga
     1381 cggcgcccag gcactcgagc ggctgcagct cgagtcgttc gacctagtcc tcatggattg
     1441 cgagatgccc gtgatggatg gattctcggc gacccgcgag ctgcgccgcg gcgaagctgc
     1501 ggagcggcgc ctgccggtca tagcgctgac cgccgatgtg acccgcgaag ggcgtagcgc
     1561 ctgcctcgcg gcaggcatgg ac
//
LOCUS       DATLKL010000379         1564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_196156, whole genome shotgun sequence.
ACCESSION   DATLKL010000379 DATLKL010000000
VERSION     DATLKL010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196156"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..564
                     /locus_tag="VM713_04965"
     CDS             10..564
                     /locus_tag="VM713_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HVH83653.1"
                     /translation="MPSPLLVTFTAGTSGSWRIDRMSAVIGEGLPLARRLSMYEHPSI
                     PTGDPVAWTLRGTTSNTRYTNHDEVTRLTARQESLQRPQATRAALIPIRKSEAWWSLA
                     QDERREIFEEQSRHIAIGMDYLPAIARRLHHCRELGEPFDFLTWFEYASADADKFEGL
                     VRRLRATREWQFVDREVDIRLSRI"
     gene            complement(545..>1564)
                     /locus_tag="VM713_04970"
     CDS             complement(545..>1564)
                     /locus_tag="VM713_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83654.1"
                     /translation="VLDAAARRRLGIPRVDTHEYARENGWMIRALARLEEVTGDSAAL
                     AAAVSAAEWVVAHRALPGGGFSHDASDKGGPFLDDTLSMGQAFLALYRASGDRRWLQR
                     ARAALDYIVTTFRDARGGFVTAAAPPGAVGVFADPVRTIEQNAAVARFTNLLHRYTGD
                     EHCREAALHAVKFLAAAVAGAEDLHADVLLADRELANAPIHITIVGGKSDPEAQALHA
                     AALGFPAGYLQIDWWDRAEGPLPNPEVRYQSSSAPPLSPARRVPARSPFTSPANSTGP
                     SPPPCTSRRRAVSRPCLLRSDGSGRPGALSDNHLHRDRGCHRISLLTEGRNEATRLTV
                     RFGSA"
BASE COUNT          278 a          495 c          516 g          275 t
ORIGIN      
        1 ggagtgccaa tgccatcacc cttgcttgtg acatttaccg ccggtacgag cggaagttgg
       61 cgcatcgacc gcatgtctgc agtcatcggc gagggcctgc cattggccag acgcctctcg
      121 atgtacgagc atccttcgat acccaccggc gaccccgtgg cctggacgct ccgcggtacg
      181 accagtaaca ctcgctatac gaaccatgac gaagtgacaa gactcacggc gcgccaggag
      241 agtttgcaaa gaccgcaagc gacacgcgct gcgctgattc caatcaggaa atccgaagcg
      301 tggtggtctc tcgcgcaaga tgagcggcgg gaaatattcg aagaacaatc gagacacatt
      361 gcgatcggta tggactatct gccggcaatc gccaggcgtc tgcatcattg ccgtgaactc
      421 ggtgagccat ttgattttct gacgtggttt gagtacgcgt cagcggacgc ggacaaattc
      481 gagggactag tgcgacggtt acgcgccacc cgcgaatggc agtttgtaga ccgtgaagtc
      541 gacattaggc tgagccgaat ctgacagtta gtctcgtcgc ttcattcctg ccctctgtga
      601 gcagggatat gcggtggcac ccgcggtcgc ggtgaaggtg attgtcactc agtgctcctg
      661 gccgaccgct gccgtctgag cgcaggaggc acggccggct gacagcgcgc cgcctactcg
      721 tacagggcgg cggtgacggt ccggtagagt tcgccgggct cgtaaacggg cttcgagcag
      781 gtactctgcg tgcaggcgaa agcggcggcg cgcttgagct ctggtaccgt acctcgggat
      841 tcggcagcgg cccctccgcg cggtcccacc agtcgatctg caggtagcca gccggaaatc
      901 cgagcgccgc ggcgtgcagt gcctgggcct cggggtcact cttgccgccc acgatagtga
      961 tgtgaatcgg cgcgttggcg agttcccggt cggcgagcag cacgtcagcg tgcagatcct
     1021 cggcgccggc gaccgccgct gcaaggaact tcaccgcgtg cagtgctgcc tcccggcaat
     1081 gctcgtcgcc agtgtaacgg tgcagcaggt ttgtgaagcg tgcgaccgca gcgttctgct
     1141 cgatggtgcg cacgggatcg gcgaacacac ccactgcgcc cggaggcgcg gccgccgtga
     1201 caaatccccc gcgggcatcg cggaaggttg tgacgatgta gtcgagcgcg gcgcgcgcgc
     1261 gctgcagcca gcggcgatcg ccgcttgcgc ggtacagggc gagaaatgcc tgtcccatcg
     1321 agagggtgtc gtcgaggaac gggccaccct tgtcgctcgc gtcgtggctg aagccccctc
     1381 ccggcagtgc gcgatgcgcg acgacccatt cggccgcgct caccgccgcc gcaagcgcag
     1441 cggaatcacc ggtaacctcc tcgagcctgg cgagtgcgcg gatcatccag ccgttctcgc
     1501 gcgcgtattc gtgggtgtcg actctcggga tcccgaggcg ccgccgcgcg gcggcatcca
     1561 gcac
//
LOCUS       DATLKL010000380         1547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_128621, whole genome shotgun sequence.
ACCESSION   DATLKL010000380 DATLKL010000000
VERSION     DATLKL010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128621"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(328..594)
                     /locus_tag="VM713_04975"
     CDS             complement(328..594)
                     /locus_tag="VM713_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HVH83655.1"
                     /translation="MARPVRKTVKEVPLAEVKDDFSRYLREAAAREIVITRHGRPAGV
                     LIGFGSEDDWLEYRLENDQRFLKRIAKARASLRAGRGIALEDIE"
BASE COUNT          239 a          498 c          521 g          289 t
ORIGIN      
        1 cgacttcacc agcagatcga gacggttgga tggcctgctt ccgttttggt gacctcgatt
       61 gactcgagcg ctgcgctcgc gccagctgct tctgagtaat gcgagcgcag ctcgatatag
      121 gcagccttct ccaggctcaa gcccagaaag tccttggcgc ttcgagtcct ccagctcctg
      181 gctgccagtc tcttgcgtta ctttcccgca ttgtcagccg ccgcgagtcg catgtgcatg
      241 cagtgggtct cttgttgaat gacccgcatt cgcggagaag cagcacgaag gctcacgttg
      301 cttcgcgacc atggttagac attgccttca ctcaatgtct tcgagcgcga taccgcgccc
      361 ggcgcgaagg ctcgcgcgag ctttggcgat gcgcttcaaa aaccgctgat cgttttcaag
      421 ccggtactct aaccaatcgt cttcagaccc aaagccaatg agaacccccg caggtctgcc
      481 gtggcgcgtg atcacaattt cccgagccgc agcctccctg agataacggg agaagtcgtc
      541 cttaacctct gcaagaggca cttcctttac ggttttccga actgggcgag ccatgatgtc
      601 gtctccgatt tcgggactat agccaacact tccaccaccc cgtttgtcac gtcatagaag
      661 acccgcacgt tcccgacccg caaccggtat tgcggccgcg acaggccacg caaccgcttg
      721 atgcggctac ggctggtctt acgcgggtcg tggcggaggt gcgtctccaa cgctcccttc
      781 actgccgagc gcaggccggc cttgagccta tcgagatctt caaccgcttc cggtgcgcgg
      841 actctggcaa acggcattct ggccagatta tagccagaac tgccgaacag tacagcaccg
      901 ccttctggct accgctccac cctatccctt gaccggtccg ccttgggatt cgattcgtcc
      961 cgcgacttct cctgcgactg cgcggggtgc gcgggcggcg gcgcaggatg cgcaggcggc
     1021 gacgccggat gctgctgcgg ctggctgggc ggcgggctcg aagcggcggg agtgctgtgc
     1081 accgcgggcg ccggcgcggt ttcgactcgg gtcggcggag tctccggccg gaacaccggc
     1141 tcggcggacg ggtgatcgtt cgtgcggggc gactccgccg gtgcgctcgg gcgctgcgag
     1201 gcccacggcg gccggtcggt gtgcgccggc agcggagcgg gcgacgaagc gtcgggcgac
     1261 cgcgtgttga ccggcgtgcg gggcacagtg ctcggggaga tcgcggggct cggggggagc
     1321 gctgggctcg cgagcgagcg agcgcgttcg ctgaggcccg gttgcgccgc tgccggcgac
     1381 gatggccgcg gcggcggcgg gagccgcgat cctttggttg ctggcagcac gtgcacttgg
     1441 gcagtgggag gaccgggttg cagtcgcgcg acgtctgagc gcgcgagcgg acggccgccg
     1501 ttcgcctgga tcgctgcgag ctgcgcatca aaggagaccg gcgcgcg
//
LOCUS       DATLKL010000381         2562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_60850, whole genome shotgun sequence.
ACCESSION   DATLKL010000381 DATLKL010000000
VERSION     DATLKL010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60850"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1738
                     /locus_tag="VM713_04980"
                     /pseudo
     CDS             291..1738
                     /locus_tag="VM713_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
     gene            1843..>2562
                     /locus_tag="VM713_04985"
     CDS             1843..>2562
                     /locus_tag="VM713_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83656.1"
                     /translation="MRRFSWVVLATACCAPGAYLEAADTPAVTPYQVTILSNANAQGN
                     SVNDFGLVSGSYTLANGALHASLWAAGRQVDLGTLGSGAALSSRVQWPVKNLLGLISG
                     ISLTDQLDPNKEGWSCGFFLNNPNFNVCLGFVWDRFSGMRPLPTLGGTNGFATGTNNF
                     AQTVGWAENSVHDPSCISPQVLQFRPVVWGPRRNQIQALPLPDGDTSGAATAINDRGQ
                     IVGIAGSCGFAVGGPTGKHAVL"
BASE COUNT          421 a          931 c          787 g          423 t
ORIGIN      
        1 ccgtgtgccc gaggtgcaga tcgggcgccg tcggatcgaa gcccgccttc acgcgcagcg
       61 gcgtgccgcg cccgagcttc ttctccagct ccggcggcgt caacacctcg ctcgcaccgc
      121 gcacgagctc tgcgatctgt ggctcgaaat tcatcccgat tcccgtgatg cccgttatgg
      181 acccgttaag gttcgatcct gcagactcaa cttacgttga ccggggatcc taaggccggt
      241 acggttcgcc cctattagaa acggataagg cactcgccta agtctagtcg atggcactgc
      301 gattccgtct tccaaaacct tcgtttgcct tcttcctcca ggccaccgcc tgcgtggtag
      361 tcgcctggtc gttatggaac ggcggactga cggccccacc gaccccgagc gggaccgagc
      421 ccgcggccgc cgcggacaca ggtcagtcga ctcccttcaa cccctttaaa gaccctgcgg
      481 ccgacatccc cttcgcactc acgagccccg ctcccgagga cccctcgctt gcgacatccc
      541 ctgccgcggc cggcgccagc actccggccg ccgctgccgc cagcgacgcg cccgctccgg
      601 tgcggctgat cggcgctgcc ggcttcgacg gcgcgcagct cgcgctctcc tcgatcgatg
      661 tcatcgtgac ccgcaatgac tcgctcgacc ggatcttccg ccgcctgcag ctgaacctcg
      721 ccgacctcgc aacgctgcgt gctctgccgg gactcaaggc gcatctcgac cgcctccgcc
      781 cgggcgaatc gctgcacttc acgcacaagg acggcgagct cttcgggctg cagcgccggc
      841 tgagtgacgc cgagacgctc gaggtgacgc gcgccgccga gggcctcaag gcgaacgtcg
      901 tcgagaaccc gctcgagacc cgcaggcgca tcgtcggcgg acgcatttac agctcgctct
      961 ttgaagcggt cgagggcgcc ggtgcccacg atcagaccgc cgtggcgctc gccgatatct
     1021 tcggttggga catcgacttc gtgctggacg tgcggcccgg ggactcgttc gtggtcacct
     1081 acgaggagat ctggcgcgac gggcactacc tcaaggacgg cccggtactc gccgcctcgt
     1141 tcgtcaacca gggacacgag taccgcgcgg tgcgttacac cgatccggcc ggcgccacca
     1201 gctattacgc gcccgacgga cgcagcatgc gcagggcgtt cctgcgcgcg ccgctcgaat
     1261 tcagccgcgt gagctccgga ttcaacaccg tgcgcctgca tccgatcctc caccgcatcc
     1321 gcgcgcacaa gggcgtcgac tacgccgccc cgatcggtac gccggtgcgt gccgcgggcg
     1381 atggcacggt gcgcttcgcc ggagtgatgg gcggctacgc aacctggtcg agatcgagcg
     1441 caccaacagc atcacgaccg tctacggaca cctgtcgcac tttgcccatg gcctccggcc
     1501 cggcacccgt gtcacccagg gaacggtcat cgcctatgtg ggaatgacgg gactcgcgac
     1561 cgggccgcac ctgcactacg agtatcgcgt gaacggggtc ttcatgaatc cccaggaggt
     1621 ggtgctgccg gccgccgccc ccatcagcgc gacctatagc gccgacttcc acgcacagac
     1681 cgacccactg ctggcatccc tcgaccaggc cgtggggccg atgctggtat cccgctaaaa
     1741 ccagcgcccc taaaaactag cgcgtccgaa ttccgccggt atccccgcac cccccggtgt
     1801 aggttcccct gcgcttcttt acagcggcaa ggaggaacgg acatgcgtcg cttctcatgg
     1861 gtcgtgctgg cgaccgcgtg ctgtgcaccg ggtgcgtacc tcgaggcagc ggatacgcct
     1921 gcagtcaccc cctaccaggt gacgatactc agtaacgcaa acgcccaggg caacagcgtc
     1981 aatgacttcg gcctcgtctc cgggagctac acgctggcca acggcgcgct gcacgcgagt
     2041 ctgtgggccg ccgggcggca ggtggacctc ggtaccctcg gcagcggcgc ggccttgagc
     2101 agcagggtgc aatggccggt gaagaacctc ctcggcctga tctccgggat ctccctgacc
     2161 gatcagctcg atcccaacaa ggagggctgg agctgcgggt tctttctcaa caacccgaac
     2221 ttcaatgtct gcctcggctt cgtatgggac cgtttcagcg gcatgcgccc gctgccgacc
     2281 ctcggtggca ccaacggctt cgccactggc accaacaatt tcgcgcagac ggtcggctgg
     2341 gcggagaaca gtgtgcacga cccgagctgc atcagcccgc aggtgctgca gttcaggccg
     2401 gtcgtgtggg gcccgcggcg caaccagatc caggcgctgc cgctgcccga cggcgacacc
     2461 tccggggccg caactgcgat caatgaccgc ggccagatcg tcggtatcgc gggcagctgc
     2521 ggctttgcag tcggcggacc cacgggcaag cacgcggtgc tg
//
LOCUS       DATLKL010000382         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_185014, whole genome shotgun sequence.
ACCESSION   DATLKL010000382 DATLKL010000000
VERSION     DATLKL010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185014"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VM713_04990"
     CDS             <1..415
                     /locus_tag="VM713_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83657.1"
                     /translation="YSPFLPGGQWVGPLAPAGTNLPVVPKFKGNVVVRYTFGSTSNVS
                     PYAQAAYVYQSQTSPQLQADIDQIIGMMPAYGISDLAAGAAIDNFNVEMFCSNCGDRR
                     AQLSRFANTNPLADNQVYILPATPRTIGIKFSQRF"
     gene            complement(488..964)
                     /locus_tag="VM713_04995"
     CDS             complement(488..964)
                     /locus_tag="VM713_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83658.1"
                     /translation="MRTIYSILIVPALAAAATCAEAQNVSVNAVITGQVVPGVYGQVV
                     IGNAPPPPVVYQQPMVVEPPPVVVGAPPDAPLYLHVPPGHAKHWNKHCREYNACDRPV
                     YFVRSPEYEPGFDMDRWRREHGHEHHGHGHDRDDHDRGDHDRGDHDRGDHDRDHVR"
     gene            complement(1067..1744)
                     /locus_tag="VM713_05000"
     CDS             complement(1067..1744)
                     /locus_tag="VM713_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HVH83659.1"
                     /translation="MCGKYILAQAAKAERALGIRHGRWEYPPSYRVLPTEQVPVAVSH
                     HGEHAAAMMRWGLIPFWAHGVPLKASTINATVERVETAPSYRDAWQRDQRCIFPMGGF
                     YEPHVNEDGSRDQFFLHLADREVFGVAGLWDRSRRSDGSWMHSCVLITVPANRLLAEI
                     HNAKQRMPAILAAEDHEAWLAGGKPEAKAALKPYPEELMVGWKVSRRLNNPRLPNDAT
                     LIEPLGS"
     gene            1836..>2066
                     /locus_tag="VM713_05005"
     CDS             1836..>2066
                     /locus_tag="VM713_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83660.1"
                     /translation="MLPWNSVERLRDHFGDYVIVVTCRHCRHSRELTPAFLARHCRAG
                     WDEPVAKIIARFRCRCGKKLVDVHVGFNKKPRG"
BASE COUNT          324 a          693 c          684 g          365 t
ORIGIN      
        1 gtattcgccg ttcctgccgg gcggtcagtg ggtcggaccg ctcgcgcccg ccggcacgaa
       61 tttgccggtg gtgccgaagt tcaagggcaa cgtcgtggtg cgctacacct tcggctcgac
      121 cagcaacgtg tcgccgtacg ctcaggcggc ctacgtctac cagtcgcaga cctcgccgca
      181 gctgcaggcg gacatcgacc agatcatcgg catgatgccg gcttacggca tctcggatct
      241 ggcggccggc gccgcgatcg acaacttcaa cgtcgagatg ttctgcagca actgtgggga
      301 taggcgcgca cagctatcgc gctttgccaa caccaacccg ctcgccgaca accaggtgta
      361 catcctgccg gcgacgccgc gcacgatcgg catcaagttc tcgcagcgct tctaaagggc
      421 gcgctcgcgc ccgactcgcc aagccggctc gcctctcgca gggcgagccg gcgcgcgagc
      481 ttccctatca gcggacgtga tcgcggtcgt ggtcgccgcg gtcgtggtcg ccgcgatcat
      541 ggtcgccgcg gtcgtggtca tcacgatcat ggccgtggcc gtggtgctcg tgcccgtgct
      601 cgcggcgcca gcggtccata tcgaagcccg gctcgtattc cggcgatctc acgaagtaca
      661 ccggccggtc gcaggcgttg tactcgcggc aatgcttgtt ccagtgcttg gcatgcccgg
      721 gcggcacgtg caagtagagc ggtgcgtcgg gcggcgcgcc caccaccacc ggtgggggct
      781 ccacgaccat cggctgctga tacaccaccg gcggcggcgg ggcgttgccg atcactacct
      841 ggccgtacac tcccggcact acctgcccgg tgatgaccgc attgacgctg acgttctgcg
      901 cctcggcgca ggttgcagcg gccgccagag ccggcacgat caggatcgaa taaatggttc
      961 gcatgaatta ttcctccagg cagtaattat gccgccgccc gcgcctctca tgacaccctg
     1021 cggccgctga acagattatg aacggcgcgg ctgctcgacc gtggcgtcag ctcccgagcg
     1081 gctcgatcag ggtggcatcg ttgggcaggc gcggattgtt gagacggcgg ctgaccttcc
     1141 agccgaccat gagctcttcg gggtagggct tcagcgccgc cttggcctcg ggtttcccgc
     1201 cggcgagcca ggcttcgtga tcctccgcgg ccagaatcgc cggcatgcgc tgcttggcgt
     1261 tatgaatctc ggccagcagg cggttggcgg gcactgtgat cagcacgcag gaatgcatcc
     1321 agctgccatc gctgcgccgc gagcggtccc acagtcccgc gacgccgaac acctcgcggt
     1381 cggcgaggtg caggaaaaac tgatcgcgcg agccgtcctc gttgacgtgc ggctcgtaga
     1441 aaccgcccat gggaaagatg cagcgctggt cgcgctgcca ggcgtcgcgg tagctcggcg
     1501 ccgtctccac gcgctcgacc gtggcgttga tcgtggaggc tttgagcggc acgccgtgcg
     1561 cccagaaggg aatgagcccc cagcgcatca tggcggcggc gtgctcgccg tgatgcgaca
     1621 ccgcgaccgg cacctgctcg gtgggcagca cccggtagct cggcggatat tcccagcgtc
     1681 cgtgccggat accgagagcg cgctcggcct tggcggcctg ggcgaggatg tacttaccac
     1741 acatgagctt gcgcgtgccg aagatgcttc agacagtatc gcacgcactt tagcactggt
     1801 tggacgcgcc gccggcgggc tgctcacact gtgccatgct gccgtggaat tcggttgagc
     1861 ggctgcgtga tcacttcggc gactacgtga tcgtcgtaac ctgccgccac tgccgccatt
     1921 cgcgcgagct gacgccggct tttctcgcgc gccattgccg cgccggctgg gacgagccgg
     1981 tcgcgaagat catcgcgaga ttccgctgtc gctgcggcaa gaagctggtc gatgtgcacg
     2041 tcggcttcaa caagaagccg cgcggc
//
LOCUS       DATLKL010000383         1906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_60856, whole genome shotgun sequence.
ACCESSION   DATLKL010000383 DATLKL010000000
VERSION     DATLKL010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60856"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1815
                     /locus_tag="VM713_05010"
     CDS             157..1815
                     /locus_tag="VM713_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83661.1"
                     /translation="MSRHPFTSRLLWAVALASCAALAAAADPPQERKAEGDSEKAEGH
                     KFEPFRPEAVTTSGSVTIGGKALSYQAIAGTLVVHPRGWDDVPRDPNAKAAAGEEGEA
                     GNPTAEASVFYVYYSRGGEGPRPVTFLYNGGPGSSTMWLHMGAFGPRRIVTSSDAHTP
                     AAPYSLINNAYSLLDATDLVFIDAPGTGFSRIAGKDKEKAFFGLDPDAYAFSEFITQF
                     LTKYGRWNSPKYLFGESYGTPRSAVVVNQLESERSVDLNGVILLSQILSFDLSPDRPT
                     GNPGVDLPYETVLPTYAATAWYHKKLPGEHANLEALLTEVEQFAMGDYARALAAGSEL
                     SASDRTTVAQKLHEYTGLPLDYILKADLRIDGGEFRQNLQSDGGLTTGRLDSRFSGPD
                     IDPLSQRAEYDPQSAALSSAYVSAFNDYARKELHYGEGKIFKPSIQTFRTWNFAHQQP
                     GQTQGPPLSPRQGVNVLPDLANAMKLNPNLKVQLNAGYFDLATPFYQGVYEMHHLPMP
                     QSLESNIEFRFYDSGHMVYAKDASLRQLHDNVADFIRRTSHAGG"
BASE COUNT          353 a          677 c          571 g          305 t
ORIGIN      
        1 cgttggtgat gagcttcacc ggcgcttacg ccgtcatagc gagcgcccgg gcgaagtaag
       61 gcacacgtct cccgcaacgc gcgcggcgca tgcggcgccc ggtccgctcg gctagactct
      121 cgcgctcccg gaggccggta ccagaaggat gagatcatga gccgccaccc attcacctcg
      181 cgtctgctgt gggcggtcgc actcgcctcg tgcgcggcgc tggctgccgc cgccgacccg
      241 ccgcaggagc gtaaagccga aggcgactcc gaaaaggcgg agggtcacaa gttcgagcct
      301 ttcaggcccg aggcggtcac gaccagtggc agcgtcacca tcggcggcaa ggcgctgagt
      361 taccaggcga tcgccggcac gctcgtcgtt cacccgcggg gctgggatga cgtgccgcgc
      421 gacccgaatg ccaaggcggc ggccggcgag gaaggcgaag ccggcaaccc gacggccgag
      481 gcctccgtct tctatgtcta ttactcgagg ggcggcgagg gtccgcggcc cgtcacattc
      541 ctttacaacg gcggccccgg ctcctccacc atgtggctgc acatgggggc cttcgggccg
      601 cgccgcatcg tgacctcatc ggacgcccac accccggctg cgccgtattc actgatcaac
      661 aatgcgtaca gcctcctcga tgccaccgac ttggtcttca tcgatgctcc gggcacgggg
      721 ttcagccgca tcgccggcaa ggacaaggaa aaggcgttct tcgggctcga cccggatgcc
      781 tacgccttca gcgagttcat tactcagttc ctcaccaagt acggccggtg gaactcaccc
      841 aagtacctct tcggcgagag ctacggcacg ccgcgctcgg cggtcgtcgt taaccagctc
      901 gaatcggagc gctcggtcga cttgaacgga gtcatcctcc tttcgcagat tctcagcttc
      961 gacctgagcc ccgatcggcc gaccggtaat cccggcgttg atcttcctta cgagacggtg
     1021 ctgccgacct acgcagctac cgcctggtac cacaagaaac tcccgggcga gcatgcgaat
     1081 ctcgaagcgc tgctcaccga agtcgagcag ttcgccatgg gggactacgc gcgcgcattg
     1141 gcggccggct cggagttgag cgccagcgat cgcaccacgg tcgcgcagaa gctccatgag
     1201 tacaccgggc tgccgctcga ctacatcctc aaggccgacc tgcgcatcga tggcggcgaa
     1261 ttccgtcaga acctgcagag cgatggcggt ctcaccaccg gccggctcga cagccgcttc
     1321 tccggtcccg acattgatcc cctgagccag cgcgccgaat acgatccgca gtccgccgcg
     1381 ctgagctcag cctacgtctc ggcgttcaac gactacgcgc gcaaggagct gcactacggc
     1441 gaggggaaga tcttcaagcc gagcatccag accttccgca cctggaactt cgcccaccag
     1501 cagcccggac agactcaggg cccgcccctc tcgccgcgcc agggcgtcaa cgtgctgcca
     1561 gacctcgcca atgcgatgaa gctcaacccg aatctcaagg ttcagctcaa cgccggctac
     1621 ttcgacctcg cgaccccgtt ctaccagggc gtctacgaga tgcaccacct gccgatgccg
     1681 cagagcctcg agtccaacat cgaattccgt ttctacgatt cggggcacat ggtatacgcc
     1741 aaggacgctt cgctcaggca gctgcacgac aacgtggcgg acttcatccg ccgcaccagc
     1801 cacgcgggcg gctaactgcg caggtacgag gcgccgttca cgtccagaat tgcacccgtc
     1861 atggccgcag gcgcgtcgag cacgcagaac gcgacgacct gcgcga
//
LOCUS       DATLKL010000384         1690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_207412, whole genome shotgun sequence.
ACCESSION   DATLKL010000384 DATLKL010000000
VERSION     DATLKL010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207412"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VM713_05015"
     CDS             complement(<1..542)
                     /locus_tag="VM713_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HVH83662.1"
                     /translation="MADTEPKLRRESPAEGGAEPKLKRESPAESRAEVLTLDRPVRDT
                     ARPRVRIIPLLITLATIVIALALGWGMWDAYMGSPWTRDGTVRVYVVTMAPEVAGRIV
                     ELPVHDNQFVHKGDLLMVIDPINYRIAVDLADAAVKQADANAQNAAIQAERRARLTTL
                     AVSQEEKQIYATQAVAAQAQL"
     gene            complement(535..753)
                     /locus_tag="VM713_05020"
     CDS             complement(535..753)
                     /locus_tag="VM713_05020"
                     /inference="COORDINATES: protein motif:HMM:NF019484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HVH83663.1"
                     /translation="MRFTEVDLFGVYVAPMSVMLVAAWVVTIALRRVASRFGLLRYVW
                     HPALFSFAVYMIVLSSMVLFLAHRVSHG"
     gene            complement(750..>1690)
                     /locus_tag="VM713_05025"
     CDS             complement(750..>1690)
                     /locus_tag="VM713_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HVH83664.1"
                     /translation="SRVALRLHVPDWLPAFVNAGRAFVTIGAVAVFWIVTEWPNGAFA
                     ITFAANGTILLAPRADQAYAAAMSLMVATALGTAFAAIIKFAVLPGSETFAAFALAIG
                     LLLVPAGAGSAQPWQTAVFFLMVVNFVPLLAPANQMSYDTVQFYNQASAIVAGTVAAA
                     ISFRLLPPLSPAFRTRRLLALALSDLRRVATGPIPRTTEDWEGRMFARLAVLPNLPDT
                     LPGARLVAARLVGTEIIELCRTAPGLGLGADLDTALEALARGDEEATTTRLARLDRLL
                     ASHPAGPVALRVRGRVLAISEAITQHASYFLAGVSR"
BASE COUNT          326 a          549 c          561 g          254 t
ORIGIN      
        1 agctgggcct gggcggctac cgcttgcgtc gcataaatct gcttctcttc ttgcgagacc
       61 gccagagtgg taagcctcgc ccggcgctcc gcctgtattg cagcgttctg cgcgttggcg
      121 tcggcctgct tgaccgcggc atcggcgagg tcgacggcga tcctatagtt gatcgggtcg
      181 atcaccatca gcaagtcgcc cttgtgcacg aactgattgt cgtgcaccgg aagctcgaca
      241 atgcggcctg cgacctccgg cgccatcgtg acgacataga ctcgcaccgt tccgtcgcgc
      301 gtccagggcg accccatata ggcgtcccac attccccaac cgagtgccag ggcaatcacg
      361 atcgtcgcca gtgtgatcag caacggaata atgcggacgc gcggcctcgc cgtgtcgcgg
      421 acgggccggt cgagcgtcaa gacctcggcg cggctctcgg ccggcgattc gcgtttcaat
      481 ttgggttcgg cgccgccctc ggccggcgat tcgcgcctta atttgggttc ggtgtcagcc
      541 atgactgact ctgtgcgcga ggaaaagcac catcgacgag agcacgatca tgtagacggc
      601 aaacgaaaag agagccggat gccagacgta acgcagcaag ccgaaccggc tcgccaccct
      661 gcgcagcgcg atggtgacta cccaggccgc gaccagcatc accgacatcg gcgcgacgta
      721 tacgccgaac aagtcaacct cggtgaacct cacctcgata ctcccgcgag gaagtaggag
      781 gcatgttggg taatcgcctc cgagattgca aggacgcggc cgcgcacccg gagagccacg
      841 ggtccggcag gatgcgaggc gagaagccgg tcgagccggg cgagccgcgt ggtcgtggcc
      901 tcctcgtctc ctcgcgccaa ggcctcgagc gccgtgtcga ggtccgcccc cagaccgagc
      961 ccgggtgcgg tgcggcaaag ctcgatgatc tcggtaccca ccagtcgcgc cgccacgagc
     1021 cgcgcgccgg gcagcgtgtc gggcagattc ggcaagacag cgagccgtgc aaacattcgg
     1081 ccctcccaat cctcggtcgt ccggggaatg gggcccgtcg caacgcggcg cagatcgctc
     1141 agtgccaagg ccagcagcct gcgggtgcgg aacgcgggcg acagcggcgg cagcagtcga
     1201 aacgaaatcg ctgcggcgac cgtccctgcg acgatcgccg acgcctggtt gtagaattgc
     1261 acggtgtcgt aactcatctg gttcgcgggc gcgagaagcg gcacgaaatt tactaccata
     1321 aggaaaaaca ccgccgtctg ccacggctgg gcggatccgg cgccggcggg aaccagcaaa
     1381 aggccgatcg ccagggcgaa ggcggcaaag gtctccgacc ccggcagcac ggcaaatttg
     1441 atgatcgccg cgaaagcggt gccgagggcg gtggccacca tgagactcat ggctgcggcg
     1501 taggcctggt ccgctcgcgg cgcgagcagg atggtgccgt tcgcggcaaa ggtgatcgca
     1561 aacgcgccgt tcggccactc ggtcacgatc caaaagaccg cgacggcgcc gatcgtgacg
     1621 aacgcgcgcc cggcattgac gaatgcgggt agccaatcgg gcacgtggag ccggagcgca
     1681 actcgagaag
//
LOCUS       DATLKL010000385         2623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_27097, whole genome shotgun sequence.
ACCESSION   DATLKL010000385 DATLKL010000000
VERSION     DATLKL010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27097"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VM713_05030"
     CDS             <1..548
                     /locus_tag="VM713_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83665.1"
                     /translation="QVETAVPAACALGLEPRRPLVVYFLAGTAAGTPIENPRQVAAYH
                     YPRQYGPASPTFARAMLAGGSDGATLLVSGTSSIVGHRTLHAGDIVAQTRETVANLRA
                     LLAEASSHAAGARFALEELAYKVYLRHERDLSACAAELSVALGPAAPLVFLRADVCRA
                     DLLVEIEGVGQAPAQAARRAQ"
     gene            complement(524..1258)
                     /locus_tag="VM713_05035"
     CDS             complement(524..1258)
                     /locus_tag="VM713_05035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVH83666.1"
                     /translation="MQAPHPPLHSYYRSESDRSAWVRDCFDRSAPDYDRLERILGLGT
                     GSRYRRRALRAAGLGTGMSVLDIGSGTGLLASAAASIVGDPKLVVGMDPSPGMIAHAR
                     VPAGLRLLSGSAEQIPAADSSADFLCMGYALRHVGDLSAAFGEFHRVLRPGSRLCILE
                     ITVPAGRIRRALLKTWLHGAVPRIATLVASQREAPLLMRYYWDTIAACVPPHTVLAAI
                     AAAGFSDVTRRVELGMFSAYCARRAA"
     gene            complement(1264..2382)
                     /gene="modC"
                     /locus_tag="VM713_05040"
     CDS             complement(1264..2382)
                     /gene="modC"
                     /locus_tag="VM713_05040"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HVH83667.1"
                     /translation="MLRVSLLKRREEFTLAASFAAPLPGAIAVFGRSGSGKTTLIDLI
                     AGLLPPDEGEVQLAGAVLTDTRRGIAVAPERRRIGYVFQDGRLFPHLTAAANLRYGER
                     RARAISHGIRFEEVVELLGLAALLHRRPHELSGGERQRVALGRALLSQPQLLLLDEPM
                     ASLDVARREEVLPYLETLRDWLSVPMLYVSHQFEEVLRLATYIVLLEEGRVLAAGPVG
                     EMSLRPELRTVLAPDLVGAVLDGLVTGVDAAGGTVELAIGAGKLQVSLRQVPLGARVR
                     VQLLARDIILATQPIEHLSVRNALLGTVTAITSDDAASVLVQVDVGGPTVLARITEAA
                     RQALRLAPGDAVWALVKAVSTRGHAFRLFGAPPGPALG"
     gene            complement(2398..>2623)
                     /gene="modB"
                     /locus_tag="VM713_05045"
                     /gene_synonym="chlJ"
     CDS             complement(2398..>2623)
                     /gene="modB"
                     /locus_tag="VM713_05045"
                     /gene_synonym="chlJ"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="part of ModCBA molybdate transporter; member of ABC
                     superfamily; inner membrane component; regulated by
                     repressor protein ModE; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HVH83668.1"
                     /translation="AFAAGLGEFGAVITFAANIPGVTRTRPLALYTAMQSPTGDPLAL
                     RLAILSFALGLTGLLGAELLVRRVRRLLGR"
BASE COUNT          406 a          912 c          965 g          340 t
ORIGIN      
        1 gccaggtcga gactgcggtc ccggcggcgt gcgcgctcgg actggagcca cgcaggcccc
       61 tggtggtgta tttcctcgcc ggcacggcgg ccggcactcc catcgagaac ccgcgccagg
      121 tcgccgccta ccattatccg cggcaatacg gcccggcgag cccgactttc gcgcgcgcga
      181 tgctcgcggg tggcagcgat ggcgcaacgc tgctggtgtc gggaacgtcg agcatcgtcg
      241 ggcatcgcac cctgcacgcg ggggacatcg tcgcgcagac gcgcgagacc gtggccaacc
      301 tccgcgcgct gctcgccgag gcgagcagcc acgccgcggg cgcaagattc gcgctcgagg
      361 agctcgctta caaggtatac ctgcggcacg agcgcgactt gagcgcgtgc gcggccgagc
      421 tgagcgtcgc gctcggaccg gcggcgccgc tcgttttcct acgcgccgac gtatgccgcg
      481 cggatttgct ggtagagatc gagggcgtcg gccaggcgcc cgctcaggct gcgcggcgcg
      541 cgcagtaggc cgagaacatc ccgagctcga cccggcgggt gacgtcgctg aagccggccg
      601 ctgcgatcgc cgccagaact gtgtgcggcg gaacgcaggc cgcaatcgtg tcccagtagt
      661 agcgcatgag cagcggcgcc tcgcgctggc ttgctacgag ggtcgcgatc cgcggcaccg
      721 cgccgtgcag ccaggtcttg aggagcgcgc ggcggatacg gccggccggg actgtgatct
      781 cgaggatgca cagccgggag ccaggccgca gcacgcggtg gaattcgccg aatgctgcgc
      841 tgagatcgcc gacatggcgc agcgcatagc ccatgcagag gaagtcggcg gagctgtccg
      901 cggcggggat ctgctcggcg ctgccgctga gcaggcgcaa tccggcaggc acccgcgcgt
      961 gcgcgatcat gcccgggctc ggatccatgc cgacgaccag cttcgggtcg ccgacgatgc
     1021 tcgccgcggc gctcgccagc aggccggtgc cggagccgat gtcgagtacg ctcattccgg
     1081 ttccgaggcc cgcggctctg agcgctcgcc ggcggtagcg ggagccggtc ccgaggccga
     1141 ggatgcgctc gaggcgatcg tagtcggggg cgctgcggtc gaagcaatcg cgaacccagg
     1201 cgctgcgatc actttcgctg cggtagtagc tgtggagcgg tgggtggggc gcttgcaagg
     1261 cagtcatcct aaggcaggtc ccggcggcgc gccgaacagg cgaaaggcgt ggccacgcgt
     1321 cgacacggcc ttcacgagcg cccatacggc atcgccgggt gcgagccgca gcgcctgccg
     1381 tgcggcctcg gtgatgcgcg cgagcacggt cggaccgccg acatccacct gcacgagcac
     1441 gctcgcggca tcgtcggacg tgattgcggt gacggtcccg agcagggcgt tgcgcacgct
     1501 cagatgctcg atcggctggg tcgcgaggat gatgtcgcgc gccagcagct gcacccgcac
     1561 gcgcgctccg agcggcactt gcctcaggct cacctgcagc ttgcctgcgc caatggcgag
     1621 ctcgaccgtg ccgccggccg catcgacccc ggtgaccagt ccatcgagca ccgcgccgac
     1681 caggtcgggc gccagtaccg tgcgcagctc gggacgcagg ctcatctccc cgaccggccc
     1741 tgcggcgagc acccgtccct cctcgagcag cacgatatag gtcgcgagcc gcagcacctc
     1801 ctcgaactgg tggctcacgt acagcatggg gacggacagc cagtcgcgca gcgtctcgag
     1861 gtagggcagc acctcctcgc gccgcgccac atcgagcgag gccatgggct cgtcgagcag
     1921 cagcagctgt ggttgcgaca gcagggcgcg gcccagggcg acgcgctgac gctcgccgcc
     1981 ggagagctcg tgcggccggc gatgcagaag ggcggcgagg ccgagcagct ccaccacctc
     2041 ctcgaagcgg atgccatggg aaatcgcccg ggcgcgccgc tcgccgtagc ggaggtttgc
     2101 agcggcggtg aggtgcggga acagccgccc atcctggaac acatagccga tgcggcgccg
     2161 ctccggcgcc acggcgatgc cacggcgggt gtcggtgagg acggcgcccg caagctgcac
     2221 ttcgccctca tcgggcggca gcagccccgc gatcaggtcg atgagcgtgg tcttgccgct
     2281 gccggagcgg ccgaagacgg cgatcgcgcc gggcagcggc gctgcgaacg aggcggcgag
     2341 tgtgaactcc tcgcggcgct tgagcagtga gacacgcagc atccccgtgc tccgacgcta
     2401 acggcccagc agccggcgta cgcgccgcac gagtagctcc gcgccgagca gtcccgtcag
     2461 cccgagcgcg aacgacagaa tcgcgaggcg caaggcgagc ggatcgcccg tgggcgactg
     2521 catcgccgtg tacagcgcca gcggccgggt gcgggtcacg ccggggatgt tggcggcgaa
     2581 ggtgatgacg gcaccgaact cgcccagacc cgccgcgaac gcg
//
LOCUS       DATLKL010000386         2437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_128689, whole genome shotgun sequence.
ACCESSION   DATLKL010000386 DATLKL010000000
VERSION     DATLKL010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128689"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VM713_05050"
     CDS             <1..315
                     /locus_tag="VM713_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVH83669.1"
                     /translation="LTAQALRALLDPRATPAPPLIDVREPWEYDTGHLPDAVNIPLGE
                     LAQRMGEVPRAGGAVFICRSGGRSLRACSLALAAGVAAPANLEGGLLAWAAEIDPTLP
                     VA"
     gene            complement(336..1091)
                     /locus_tag="VM713_05055"
     CDS             complement(336..1091)
                     /locus_tag="VM713_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HVH83670.1"
                     /translation="MAHSQKLAYHDPKFIDSDEGRPIRIVSEYLAPLRTFCEEGVTAT
                     VVFFGSARARSGGVLARYVEEATELARLVTEWGRGLPGGERLIVCSGGGPGIMEAANR
                     GATLAGGRSIGLNIGLPQEQRPNPFITPALSFQFHYFFMRKLWFAHLAHALVAFPGGF
                     GTLDELFEILTLAQTRKLDRSIPVLLYGSAYWKEVINFEALVRHGTIAAADLELFCFV
                     DEPQAALALLKRRIALAPEPPGAPCFAKSRTAD"
     gene            1238..2149
                     /locus_tag="VM713_05060"
     CDS             1238..2149
                     /locus_tag="VM713_05060"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HVH83671.1"
                     /translation="MLRPHGRSIACLFAAALAPPWVSAVAADPASGAAALPPLDAQTS
                     LLVVAPHPDDETLCCAGVIQRVVRAGGRTSVVWITSGDGSFLDLLLVEKSLTGSAARQ
                     RALAARRMREARTAAALLGVRDDGQLFLGYPDGGVLELLTAHHDTPYTSRSTAAAAVP
                     YPDALFPGHAYTGASLEHDFAAVLERVHPTLVLAPSPRDSHPDHRAAALLTLRVLGSR
                     GELRALRCWIVHGGAGWPSPRELSPGLPLPRAPLLAGVPLVSFELTPAEEDRKLAAVR
                     AHATQLTTLAPFLLAFVRSSEWFSAAP"
     gene            complement(2168..>2437)
                     /locus_tag="VM713_05065"
     CDS             complement(2168..>2437)
                     /locus_tag="VM713_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HVH83672.1"
                     /translation="HAKMIVCDDWVSIGSSNLDRWSFKWNLEANQEVADPRVADAAAA
                     LFAKDLAVSSVLSRRHWPQRAWLDRLRERIAGALDRWLEGWRRPP"
BASE COUNT          346 a          878 c          840 g          373 t
ORIGIN      
        1 ctcacggcgc aggcgctgcg tgcgctgctc gatccgcggg ccacaccggc tccgccgctc
       61 atcgacgtgc gcgagccctg ggaatacgac accggccacc tgccggacgc cgtcaacatt
      121 cccctcggcg agctcgcgca gcggatgggc gaagtcccac gcgcgggtgg cgcggtgttc
      181 atctgccgca gcggcggccg cagtctgcgt gcctgcagcc tggcgctcgc ggcgggcgtc
      241 gccgcgccgg ccaacctcga ggggggactg ctcgcctggg cagccgagat cgatccgacg
      301 ctgccggtcg cctgaggctt attgatttcg gcccatcaat cagcagtgcg cgacttagcg
      361 aagcacggcg cgccgggcgg ctcgggcgcg agcgcaatgc ggcgcttgag cagtgcgagt
      421 gccgcctgcg gctcgtcgac gaagcagaag agctcgaggt ccgctgcggc gatggtgccg
      481 tggcgaacca gagcttcgaa gttgatcact tctttccagt aggctgagcc gtacagcagc
      541 accggaatcg agcgatcgag cttgcgtgtc tgcgccagcg tcaggatctc gaacagctca
      601 tcgagcgtgc cgaagccacc ggggaacgcc accagagcgt gggcgaggtg ggcgaaccag
      661 agcttgcgca tgaagaagta gtggaactgg aatgacagcg ccggcgtgat gaacgggttg
      721 ggacgctgct cctgcggcag gccgatgttg agtcctatac tgcggccgcc ggcgagcgtc
      781 gccccgcggt tggcggcttc catgatcccc ggcccgccgc ccgagcacac gatgaggcgc
      841 tcaccgcccg gcaggccacg gccccactca gtcacgaggc gggcgagctc ggtcgcctcc
      901 tcgacgtagc gggccagcac gccgccgctg cgggcgcgcg ccgagccgaa gaacaccacc
      961 gtggcagtga cgccctcctc gcaaaaggtg cgcaggggcg cgaggtactc ggacacgatc
     1021 cgtatcgggc gcccctcgtc cgagtcgatg aatttcggat cgtggtacgc aagcttctgg
     1081 ctgtgcgcca tgacactaga gctccgcgat cgcggcggtc gccgcgtgga gcgttagtgt
     1141 gtcaagtcgc ggcgggcccg tcatccgctc cccgtaacgg gcgctccctc gcaccgctcc
     1201 ggcccctcgg gtccgactgc gctaaggtac gatgcgcttg ttgcgccctc atggcagaag
     1261 cattgcgtgc ctgttcgctg cggcgctcgc cccgccgtgg gtctccgccg tggcggcgga
     1321 tccagccagc ggcgcagccg cgctgccgcc gctcgacgcg cagacttccc tgctggtggt
     1381 cgcaccccat ccggacgatg agacgctgtg ctgtgcggga gtcatccagc gggtcgtgcg
     1441 cgccggtggc cgcacgagcg tcgtatggat cacgagcggc gatggctcgt tcctcgatct
     1501 gttgctggtc gaaaagagtc tcaccggaag tgctgcccgg cagcgcgcgc tggccgcgcg
     1561 gcgcatgcgc gaggcgcgca cggcggcagc gctcctcggc gtgagggacg acgggcaact
     1621 gttcctcggt tatccggacg gcggcgtgct cgagctcctc accgctcacc acgacacgcc
     1681 ttacacctca cgctccaccg cggccgccgc ggtgccctac ccggatgcgc tctttcccgg
     1741 ccacgcctac acgggtgcga gcctcgaaca cgacttcgcg gcggtgctcg agcgggtgca
     1801 tccgacgttg gtgttggcac cgagcccgcg cgactcgcat cccgatcacc gcgcggccgc
     1861 tctgctcacg ctgcgcgtcc tggggagccg cggcgagctg cgcgcgctgc gctgctggat
     1921 cgtgcacggc ggcgcaggct ggccgagtcc gcgcgagctg tcgccgggtc tgccgctccc
     1981 gcgcgccccg ctcctcgccg gtgtgccact cgtctccttc gagctcacgc cagcggagga
     2041 ggaccgcaag ctcgccgcgg tgcgcgccca cgcgacgcag ctcacgacgc tggcgccatt
     2101 cctgctggct ttcgtgcgca gcagcgagtg gttctccgcg gcaccgtgaa gcgcaccaag
     2161 gctcacctca cggcgggcgc cgccacccct cgagccagcg atcgagcgcc cccgcgatcc
     2221 gctcgcgcag ccggtcgagc caggcgcgct gtggccagtg gcggcggctg aggacgctcg
     2281 agacggccag atccttcgca aacagcgccg ccgcggcgtc cgccaccctg ggatcggcca
     2341 cctcctggtt cgcctcgagg ttccacttga agctccagcg atcgagattg ctcgagccga
     2401 tcgacaccca gtcgtcgcac acgatcatct tggcgtg
//
LOCUS       DATLKL010000387         1686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_105990, whole genome shotgun sequence.
ACCESSION   DATLKL010000387 DATLKL010000000
VERSION     DATLKL010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105990"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..919
                     /locus_tag="VM713_05070"
     CDS             293..919
                     /locus_tag="VM713_05070"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HVH83673.1"
                     /translation="MLAWLIDLCLHLDRHLVELLAAYHLWIYPILFAVIFAETGLVVT
                     PFLPGDSLLFAVGALAAVDTSGTLTAPLASLTLALAAVLGNTVNYGIGRAIGPPAFSG
                     RYRLLKVEYLRRTEQFFRRYGGLAIFLSRFMPIIRTCAPFVAGVGRMPYARFLAWNLA
                     GGCCWVLLFVWGGYLFGNIPLVKQHFGLVTIAIILASLVPLASALRRR"
     gene            complement(924..1322)
                     /locus_tag="VM713_05075"
     CDS             complement(924..1322)
                     /locus_tag="VM713_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HVH83674.1"
                     /translation="MPYVHIVLALALVEFFFFCLAVARARDTYQVPAPATTGHAVFER
                     YFRAQMNTLEQLIIFVPSIILFGAYVSAWLAAALGVVFIIGRAVYFRGYVRAAEGRHR
                     GFMLSVIPNLTLLGGGLLGAAWKLIRPALG"
     gene            1362..>1686
                     /locus_tag="VM713_05080"
     CDS             1362..>1686
                     /locus_tag="VM713_05080"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HVH83675.1"
                     /translation="MAERVRGYAHRVDIAADADRVWRAIIEPESLARWCSPEAELQPR
                     QGGLFRARVDRVTELEAHIDVFEKSRRVRLIYLPNPAWPRTESVLTDDLLIEAVPGGT
                     VVRLLG"
BASE COUNT          270 a          582 c          543 g          291 t
ORIGIN      
        1 gcacctgacg cgccacctcg tcccgtagtt aattggctga aattgcctgc ggcaatttca
       61 gcggaattag cgcatcactt cgcacaaacg gcgggcgaaa agcggggttt tcgcccgcga
      121 ggcaagccgg tacctgcggc aatttcagca gaattagcgc atcacttcgc acaaacggcg
      181 ggcgaaaagc ggggcttgat gcgccttcgc tgcgatcact tcagcagccg agcactccgg
      241 agcaaggtcg gcgcactggt tttcgcccgc gagaccgcta ttctctcgcg ccatgctcgc
      301 ctggctcatc gatctgtgcc tgcatctcga ccgccacctg gtcgagctcc tcgcggccta
      361 tcacctgtgg atctacccga tcctgttcgc cgtgatcttt gccgagacgg ggctcgtcgt
      421 gacgccgttt ctgccgggcg actcgctgct gttcgccgtc ggcgcactcg cggcggtcga
      481 cacgagtggc actctgaccg cgccgctcgc gagcctgacg ctggcgctcg ccgcggtgct
      541 cggcaacacg gtgaattacg gcatcggtcg tgcgatcggc ccgcccgctt tctcgggccg
      601 ctaccgcctg ctcaaggtcg agtacctgcg ccgcaccgag cagttcttcc gccgctacgg
      661 cgggctcgcg atcttcctgt cgcgtttcat gcccatcatc cgcacctgcg cgccgttcgt
      721 cgccggcgtc ggccgcatgc cctacgcgag gttcctcgcc tggaacctcg ccgggggctg
      781 ctgctgggtc ctgctcttcg tctggggcgg gtacctcttc ggcaacatcc cgctggtgaa
      841 gcagcatttc ggcctcgtga cgatcgcgat catcttggcc tcgctcgtgc cgctcgcgag
      901 cgcgctgcgg cggcgttgag gagtcagccg agcgccggcc ggatgagctt ccaggccgcg
      961 ccgagcaatc ctccaccgag gagcgtgagg ttcgggatga cgctcagcat gaagccccgg
     1021 tggcgcccct cggcagcgcg cacgtagccc ctgaagtaga ccgcccggcc gatgataaac
     1081 accaccccca gcgcggccgc gagccacgca ctcacataag cgccaaaaag gatgatcgac
     1141 ggcacgaaga tgatgagctg ctcgagcgta ttcatctgcg cccggaaata gcgctcgaac
     1201 accgcgtggc cggtggtggc cggcgccggc acctggtagg tatcccgcgc gcgggcgacc
     1261 gcgaggcaga agaagaagaa ctcgacgagg gcgagcgcga gcacgatgtg aacgtatggc
     1321 atggattccc cctgcgccca gcatagatgc aaaatgcgcc catggccgaa agagtccgtg
     1381 gttacgcgca ccgggtcgac atcgccgccg acgccgaccg ggtctggcgg gcgatcatcg
     1441 agcccgagag cctcgcccgc tggtgctcgc ccgaggctga gctgcaaccg cgccagggcg
     1501 gcctgttccg cgcccgcgtc gaccgggtga ctgagctcga ggcgcacatc gacgtgttcg
     1561 agaagagccg ccgggtacgg ctgatttatc tgccgaaccc tgcctggccc cgcaccgagt
     1621 cggtgctcac ggacgacctc ctaatcgagg ccgtgccggg gggcacggtg gtgcggctcc
     1681 tcggct
//
LOCUS       DATLKL010000388         1705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_83463, whole genome shotgun sequence.
ACCESSION   DATLKL010000388 DATLKL010000000
VERSION     DATLKL010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83463"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VM713_05085"
     CDS             <1..253
                     /locus_tag="VM713_05085"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PHB accumulation regulatory domain-containing
                     protein"
                     /protein_id="HVH83676.1"
                     /translation="RRLVIERIDFVVIDKKTQGDITRAILLQVIAEQEHGSDPLMSRD
                     FLSQVIRSYGVATRGMVGSYLEQSLRQFASQTAGRPPQG"
     gene            complement(347..>1705)
                     /gene="pnp"
                     /locus_tag="VM713_05090"
     CDS             complement(347..>1705)
                     /gene="pnp"
                     /locus_tag="VM713_05090"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017391359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HVH83677.1"
                     /translation="VSLQTQAALGEAYNITEKQQRHTRLGEIKTQVLAALTGGESPKW
                     TKEAVESVLFRLESNIVRQRILNGEPRIDGRDTRTVRPISVAVGVLPRTHGSALFTRG
                     ETQALVVTTLGTTRDAQIIDGLEGERREPFMLHYNFPPFSVGETGMMGSPKRREIGHG
                     NLARRGVYAVMPDMTTFPYVIRVVSEILESNGSSSMASVCGTSLSLMDAGVPIKAPVA
                     GVAMGLVLEGSRYKVLTDILGDEDHLGDMDFKVAGTKDGVTALQMDIKVEGVTPEIMR
                     VALEQAREGRLHILGEMNRVIHQPREKMSEWAPSIITLKIDPEKIRDVIGKGGAVIRQ
                     ITEETGTTIDIESDGTVKIASVLGTAGREAQRRIELITADVEVGRIYEGKVARLMDFG
                     AFVTILPGRDGLVHISQISNERVERVSDKLREGDVIRVKVLEVDRQGRIRLSMRDVDA
                     PH"
BASE COUNT          258 a          595 c          549 g          303 t
ORIGIN      
        1 ccgccggctg gtgatcgagc gcatcgactt cgtcgtgatt gacaagaaga cccaggggga
       61 catcacccgc gccatcctgc tgcaggtgat cgccgagcag gagcacggca gcgacccgct
      121 gatgagccgc gacttcctct cgcaggtgat ccgctcctac ggcgtggcca cacgcgggat
      181 ggtcggcagc tatcttgagc agagcctgcg ccagtttgcc agccagaccg ccggacgacc
      241 gccgcagggc tgagcgcgcg ccctctagct tagccgttag cttagggccg tgggcctgtc
      301 ggcaaacgcc gcaggcgtgc cgtcacacgc gcccaaccgg cctgattcaa tgaggtgcgt
      361 cgacgtcgcg catcgacagg cggatacgcc cctgccggtc gacctctagg accttaacgc
      421 ggatcacgtc gccctcgcgc agcttgtcgc tgacgcgctc gacgcgctcg ttcgagatct
      481 gcgaaatgtg caccagcccg tcgcgccccg ggaggatcgt gacgaaggcg ccgaagtcca
      541 tgaggcgcgc caccttgccc tcgtagatgc gccctacctc cacatcggcg gtgatgagct
      601 cgatgcgccg ctgcgcctcg cggcccgcgg taccgaggac cgaagcgatc ttcaccgtgc
      661 cgtcgctctc gatgtcgatg gtggtgccgg tctcctcggt gatctgccgg atgaccgcgc
      721 cgcctttgcc gatgacgtcg cggatctttt ccggatcgat cttgagcgtg atgatcgagg
      781 gcgcccactc cgacatcttc tcgcgcggct ggtgaatgac gcgattcatc tcgccgagga
      841 tgtgcaggcg gccttcgcgc gcctgctcga gcgccacgcg catgatctcg ggcgtgacac
      901 cctcgacctt gatgtccatc tgcagcgcgg tgacgccatc cttggtgccc gcaaccttga
      961 agtccatgtc gccgaggtgg tcctcgtcgc cgaggatgtc ggtgaggacc ttgtagcgcg
     1021 agccctcgag caccagtccc atggcgacgc cagccaccgg cgccttgatc ggcacaccgg
     1081 cgtccatcag cgacagcgac gtgccgcaca cgctcgccat cgagctcgag ccgttcgact
     1141 cgagaatctc cgacaccacg cggatcacgt acggaaaggt agtcatgtcg ggcatcacgg
     1201 cgtacacgcc acgccgcgcc aggttgccgt ggccgatctc gcgccgcttc ggcgatccca
     1261 tcatgcccgt ctcgcctacc gagaacggcg ggaagttgta gtgcagcatg aagggctcgc
     1321 gccgctcgcc ctcgagtccg tcgatgatct gggcatcacg cgtggtcccg agcgtggtga
     1381 ccaccagcgc ctgggtctcg ccgcgggtga agagcgccga gccgtgcgta cgcggcagta
     1441 cgccgaccgc cacactgatc ggccgcacgg tgcgcgtgtc gcgcccgtcg atgcgcggct
     1501 cgccgttgag gatccgctgg cgcacgatgt tgctctcgag cctgaagagc acgctctcga
     1561 ccgcctcttt ggtccacttc gggctctcgc cgccggtgag cgcggccagc acttgcgtct
     1621 tgatctcgcc gaggcgcgtg tggcgctgct gcttctcggt gatgttgtag gcctcgccga
     1681 gcgccgcctg cgtctgcagg ctgac
//
LOCUS       DATLKL010000389         1708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_207456, whole genome shotgun sequence.
ACCESSION   DATLKL010000389 DATLKL010000000
VERSION     DATLKL010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207456"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VM713_05095"
     CDS             <1..318
                     /locus_tag="VM713_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83678.1"
                     /translation="EHPHGPPEERRAHPPAAAASLIPASGPLYWGTIRKVEGQRVELE
                     LRNGSRLTVDVSKVAPTASSDTGAIGHTLAVSGTMGANKVFVASGLWRIKRHNPWGPD
                     RER"
     gene            complement(348..1550)
                     /locus_tag="VM713_05100"
     CDS             complement(348..1550)
                     /locus_tag="VM713_05100"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HVH83679.1"
                     /translation="MPHLPASRLALLLMALFFSGVSAAAAAGEGQAARPRARDLGVPF
                     DGTPGPLNAITDVEGVLVGQTTLISGNGKLVVGQGPVRTGVTAVLPRGAASMQRFSFA
                     GWYSQNGNGEMTGTTWVEESGFLEGPVLITNTHSVGVVRDAVIAWRVAHGPPDATTSW
                     WSLPVVAETWDGWLNDINGFHVKPEHVFHALDSARSGPVEEGAVGGGTGMICNGFKGG
                     IGTASRRLTDKDGGYLLGVLVQCNYGRRPNLRIAGIPVGREIESEDPYAFVPFELAER
                     GSIIVVVATNAPLLPHQLKRIARRVSLGLGRTGSIAGNGSGDIFVAFSTANPAASDSG
                     HVLDLKMLPNDSMDPLFAATVQATEEAVVNAMVAATDMTGIDGHHVRALPHEELRAVL
                     AKYNRLVK"
BASE COUNT          285 a          590 c          582 g          251 t
ORIGIN      
        1 gagcacccgc acgggcctcc agaggagcgc agggcccacc cgcccgcagc ggcggcatcg
       61 ctcattccgg cctccggccc cctctactgg ggaacgatcc gcaaggtcga ggggcagcgc
      121 gtcgagctcg agctgcgcaa cggctcgcgc ctgacggtcg atgtctcgaa ggtcgcgccg
      181 acggccagct ccgacaccgg cgcgatcggg cataccctgg ctgtcagcgg aacgatgggt
      241 gcgaacaagg tgttcgtcgc gagcggcctg tggcgtatca aacgccacaa tccctggggc
      301 ccggaccgcg agcgttgatg cggcgcgctc ccggctagcc gcttcgacta tttgacgagc
      361 cggttgtatt tggcgagcac cgcacgcagc tcctcatgcg gcagcgcccg cacgtgatgg
      421 ccatcgatgc ccgtcatgtc ggtcgcggcg accatggcgt tgacgacggc ctcctcggtg
      481 gcctgtacgg tcgcggcgaa cagcgggtcc atcgaatcat tcggcagcat cttgaggtcg
      541 agcacgtggc ccgagtcgct cgccgccgga ttggcggtcg agaacgcgac gaagatgtcg
      601 cccgagccgt tgccggcgat gctgccggtg cgcccgagtc cgagcgacac gcgccgggcg
      661 atacgcttga gctgatgcgg cagaagcggt gcgttggtgg ctaccaccac gatgatcgag
      721 ccacgctcgg cgagctcgaa gggcacgaag gcgtaagggt cttcgctttc gatctcgcgt
      781 ccgacgggaa tcccggcgat gcgcaggttc ggccgcctgc catagttgca ctgcacgagc
      841 acgccgagga gatagccgcc gtccttgtcc gtgagccggc gcgaggcggt gccgatgccg
      901 cccttgaagc cgttgcagat catgccggtg ccgccgccca cggcgccttc ctccaccggg
      961 ccggagcggg cgctgtcgag ggcgtgaaac acatgctcgg gtttgacatg aaaaccgttg
     1021 atgtcgttga gccagccgtc ccaggtttcg gcgacgaccg gcagcgacca ccaggaggtg
     1081 gtcgcatccg ggggcccgtg cgcgacgcgc caggcgatca ccgcatcacg caccacgccg
     1141 acgctgtggg tgttggtgat gagcaccgga ccctcgagaa agcccgattc ctcgacccag
     1201 gtggtgccgg tcatctcgcc gttgccgttc tgcgagtacc agccggcgaa ggagaagcgc
     1261 tgcatggagg cggcgccgcg cggcagcacg gcggtgactc ccgtgcgcac cgggccctgt
     1321 ccgacgacca gcttgccgtt gcctgagatg agcgtggtct gacccaccag cacgccctcg
     1381 acgtcggtga tggcgttgag cggccccggc gtcccatcga agggcacgcc caggtcacgc
     1441 gcccgcggtc tcgccgcttg cccctcgccc gccgcagctg cagcgctcac tcctgaaaaa
     1501 aacaacgcca tcaataagag cgcgagacgg gacgctggca gatgcggcat gtcgctctcc
     1561 tcaggtcgca agcgccggcc gccgcacgat cgcgtaccac cagtagaccg gcacccccac
     1621 ggcgcaaggt accagcgccc agaggagcga cttgccgccg atgccgcccg aggcactcca
     1681 gagcgcaatc acgatggcga cgaggagg
//
LOCUS       DATLKL010000390         1858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_151354, whole genome shotgun sequence.
ACCESSION   DATLKL010000390 DATLKL010000000
VERSION     DATLKL010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151354"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VM713_05105"
     CDS             complement(<1..453)
                     /locus_tag="VM713_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVH83680.1"
                     /translation="MARNVLGIDVGGSSIKAGLFDVDSGVVTGGLISAHTPQPSTPKA
                     MMPALASLAARLPGAVGGVGIAFPSVIKQGTARTAANVDHAWIGTDGAALAARALGRP
                     ALFLNDADAAGLAEMRWGAGRGESGTVIMVTLGTGIGTALFCDGKLYPN"
     gene            complement(464..1312)
                     /locus_tag="VM713_05110"
     CDS             complement(464..1312)
                     /locus_tag="VM713_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVH83681.1"
                     /translation="MKPRGLFTRSALVLGLVFLYVPIISMIVFSFNNSRLVTVWDAAH
                     SPTLKWYGVLMHNEQILTAAWLSIRIAIIAASASVVLGTLAGVALARLGAFRGRLLLA
                     GMVTAPIVMPEVITGLSLLLMFVALGQLIGWPKGVGAVTIALAHITFCMAYVTIVVQS
                     RLAGFDESLEEAAMDLGARPLKVFLRITLPLILPAIASGWLLAFTLSWDDLVITQFVA
                     GPGSSTLPMVVFSKVRFGVTPDVNALATILVLLVATGIAISTVWMGYQQRRRERDLQL
                     AAAANL"
     gene            complement(1309..>1858)
                     /locus_tag="VM713_05115"
     CDS             complement(1309..>1858)
                     /locus_tag="VM713_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVH83682.1"
                     /translation="LMLIVLPFWTSFLLRVYAWIGLLNNNGVINTVLLWLGVIHRPLV
                     MMQTDFAVYIGIVYSYLPFMILPLYANLEKHDQALLEAAADLGARPLRAFLRITLPQS
                     LPGIAAGSLLVFIPAVGEYVIPTLLGRPDQLMIGRVLSDEFFENRDWPVASAVAIVIL
                     LLLVVPILLFQRLERRQLEVEP"
BASE COUNT          358 a          614 c          642 g          244 t
ORIGIN      
        1 gttggggtag agcttgccgt cgcagaacag cgccgtgccg atgccggtgc cgagagtcac
       61 catgatgacc gtgccgctct cgccccggcc cgcgccccag cgcatctcgg cgaggccggc
      121 cgcgtccgcg tcgttcagga acagcgccgg ccgcccgagc gcgcgcgcgg cgagggcggc
      181 cccatcggta ccgatccagg cgtgatcgac gttggcggcg gtgcgcgccg tgccttgctt
      241 gatgacgctc gggaaggcga tgccgacgcc gccgacggct cccggcaggc gtgccgcgag
      301 cgaggcgagc gccggcatca tcgccttggg ggtcgagggc tggggagtgt gcgccgagat
      361 cagcccgccg gtgaccaccc cggagtcgac gtcgaacagg ccggccttga tcgaggatcc
      421 gcccacatcg attccgagca cgttgcgtgc catgtgcgac aactcacagg ttggcggcgg
      481 ccgcgagctg caggtcgcgc tcgcgccggc gctgttggta acccatccac acggtcgaga
      541 tggcgatgcc ggtcgcgacc agcagcacga ggatggtggc gagcgcgttc acatcgggcg
      601 tcactccgaa gcgcaccttg gaaaacacca ccatgggcag cgtgctcgag ccaggtcccg
      661 cgacgaactg cgtaatgacc agatcatccc acgagagcgt gaacgccagg agccatcccg
      721 aggcgatcgc cggcaggatg agcggcaagg tgatgcgcag gaacaccttg agcggccggg
      781 caccgaggtc catagccgcc tcctccagcg actcatcgaa cccggcgagc cgcgactgca
      841 ccacgatggt cacgtaggcc atgcagaagg tgatgtgggc aagcgcgatg gtgacggcac
      901 cgacaccctt cggccagccg atgagctgcc ccagcgcgac gaacatgagc agaagggaga
      961 gtccggtgat gacctcgggc atgacgatcg gagcggtcac catgccggcg agcagcagcc
     1021 gcccgcgaaa agcgccgagg cgcgccagcg ccacgccggc cagcgtcccg agcaccacgg
     1081 aggcgctcgc ggcgatgatc gcgatgcgga tcgacagcca ggcggcggtc aggatctgct
     1141 cgttgtgcat cagtaccccg taccacttga gcgtcggcga gtgcgcagca tcccacactg
     1201 tgacgaggcg cgaattgttg aaggaaaaca cgatcatcga gatgatcggc acgtagagga
     1261 acaccaggcc gagcaccagc gcgctgcggg taaagagccc gcgcggcttc atggctccac
     1321 ctccagctgt cgccgctcga ggcgctggaa cagcaggatc gggacgacca gcagcagcag
     1381 gatcacgatc gcgactgcgc tcgccaccgg ccagtcgcgg ttctcgaaga actcatccga
     1441 cagcacccgg ccgatcatca gctggtccgg gcgcccgagg agtgtcggga tcacgtactc
     1501 gcccaccgcc gggatgaaca ccaggagcga ccccgcggcg atgccgggaa gggactgcgg
     1561 cagcgtgatg cgtaggaacg cccgcagtgg ccgcgcgccc aggtccgccg ccgcctcgag
     1621 cagcgcctgg tcgtgcttct cgaggttggc atacagcggc aggatcatga acggcaggta
     1681 cgaatagacg atgccgatgt agaccgcgaa gtcggtctgc atcatcacca gcggccggtg
     1741 gatgacgccg agccacagca gcaccgtgtt gatgacgccg ttgttgttga gaagcccgat
     1801 ccaggcgtac acgcgcagca ggaacgaggt ccagaacggc agcacgatca gcatcagt
//
LOCUS       DATLKL010000391         2031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_49550, whole genome shotgun sequence.
ACCESSION   DATLKL010000391 DATLKL010000000
VERSION     DATLKL010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49550"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VM713_05120"
     CDS             complement(<1..569)
                     /locus_tag="VM713_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HVH83683.1"
                     /translation="MARIDAFLKLGTQQGCSDVHLAVGVPPMLRLYGDLLPIKFRDLR
                     PGELEGYIGEILTTSQNEHFAKGNDLDFSYVSADGGRFRVNVYRKDTGIGATFRAIPS
                     TVPTLASLNLPPVVTKLCDFHQGMVLVTGSTGTGKSTTLAAMIDHLNSTRKLNIISLE
                     DPIEFVHTSKQSQVIQRELGTHIPSFADGV"
     gene            complement(742..>2031)
                     /locus_tag="VM713_05125"
     CDS             complement(742..>2031)
                     /locus_tag="VM713_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVH83684.1"
                     /translation="LHTGSHGKAMPIVVRALGRLGDSKLVEELLPLVSRPEREIRIEA
                     MQALSKVADESRADQVRQELQAQAASADQTISRMAAAAVTELDNRIAGVVPTAPGLPG
                     PAAAPTIPPTAAPTGVRPPQAGAPEPTRTVLINDQARRQVEAAAAAASRLDIQTLKSG
                     DIIEGRYKYIDRIGRGAFGTVLLMEDTVVDERLILKFLNPNVSQDEEVMKRFVHELRY
                     SRKITHKNVIRIYDFLYIQGLYAISMEYFPSHTLGSEVVNEKPVELKRAMQFGIDICT
                     GMTVAHQQGIVHRDLKPANVLIDQEGLLKIVDFGVAAAQREGDTQLTKTGYVIGSPKY
                     MAPEQILGKKVDERADIYALGVMLYEMVTGVPPYSRGDHMSVMYQHVQGKARVPQDLN
                     PKLPPGLSDLVMKAMAVDKAKRFQTMDELRLALERFR"
BASE COUNT          310 a          640 c          713 g          368 t
ORIGIN      
        1 accccgtcgg caaagctcgg gatgtgggtg ccgagctcgc gctgaatgac ctggctctgc
       61 ttgctcgtgt gcacgaactc gatcggatcc tccaggctga tgatgttgag cttacgggtc
      121 gagttcaggt gatcgatcat cgccgcgagc gtggtcgact tgccggtgcc ggtggagccg
      181 gtgacgagca ccatgccctg gtggaagtcg cacagcttgg tgacgaccgg cggcaggttc
      241 aggctcgcaa gcgtcggtac ggtcgagggg atggcacgga aggtggcacc gatgccggtg
      301 tccttgcgat agacgttgac gcggaagcgc ccgccgtcgg cggacacgta ggagaagtcg
      361 aggtcgttgc ccttggcgaa gtgctcgttc tgggaggtgg tcaggatctc gccgatgtag
      421 ccctcgagct ccccgggccg caggtcgcgg aatttgatcg gcagcaggtc gccgtacagg
      481 cgcagcatcg gcggcacgcc gaccgcgagg tgcacgtccg agcacccctg ctgcgtgccg
      541 agcttcagga acgcatctat acgcgccacg cgacgacccc tgcccccaga tagcgacgat
      601 tgctgctgtg tagcccgccg caggccgcag gatcaacagc ttgacgacgg aaatttgacg
      661 taacgcacga agtttgtcca ttggcgggcc cccgcacggc ggttgggggc cgcccggcgc
      721 ccagctggcg cgtgcgcgcc cttaccggaa ccgctcgagg gcgagccgca gctcgtccat
      781 cgtctggaag cgcttggcct tgtcgaccgc catcgccttc atgaccagat ccgagaggcc
      841 gggcggcagc ttgggattga ggtcctgcgg gacacgcgcc ttaccctgga cgtgctggta
      901 catcaccgac atgtgatcgc cgcgcgaata gggcggcacg ccggtcacca tttcgtagag
      961 catgacgccg agcgcgtaga tgtcggcccg ctcgtcgacc ttcttgccga ggatctgctc
     1021 gggggccatg tacttcggcg agccgatgac gtagccggtc ttggtgagct gcgtgtcgcc
     1081 ctcacgctgc gccgccgcga cgccgaagtc cacgatcttg agcagcccct cctggtcgat
     1141 cagcacgttg gcgggcttca ggtcgcgatg cacgatcccc tgctggtgcg ccacggtcat
     1201 gccggtgcag atgtcgatgc cgaactgcat ggcgcgtttc aattcgaccg gcttctcgtt
     1261 gacgacctcg ctgccgagcg tgtgcgaagg gaagtactcc atcgagatgg cgtacagacc
     1321 ctggatgtag aggaagtcgt agatgcggat gacgttcttg tgggtgatct tgcgcgagta
     1381 acgcagctcg tggacgaagc gcttcatcac ttcctcgtcc tgcgagacgt tcgggttgag
     1441 gaacttgagg atgaggcgct catcgaccac cgtgtcctcc atcaacagca cggtgccgaa
     1501 ggcgccgcgc ccgatgcggt cgatgtactt gtaacggccc tcgatgatgt cgcctgactt
     1561 gagcgtctga atgtcgagcc gtgaggcggc ggccgccgca gcctcgacct ggcgccgtgc
     1621 ctggtcgttg atcagcaccg tgcgggtagg ctcgggagcc ccggcctgcg ggggccttac
     1681 gccggtgggc gccgcagtgg gcgggatggt cggcgcggcc gcggggccgg ggaggccggg
     1741 tgccgtggga acgacgccgg cgatgcggtt gtcgagctcg gtgacggccg ccgcggccat
     1801 gcgcgagatg gtctgatccg ccgaggcggc ctgcgcctgc agctcctggc gcacctggtc
     1861 ggcgcggctc tcgtccgcga ccttcgacag cgcctgcatc gcctcgatgc ggatctcgcg
     1921 ctccgggcgt gacaccagcg gtaacagctc ctcgaccagc ttgctgtcgc ccagcctgcc
     1981 gagggcgcgt accacgatcg gcatggcctt gccgtggctg ccggtgtgca g
//
LOCUS       DATLKL010000392         3915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_117452, whole genome shotgun sequence.
ACCESSION   DATLKL010000392 DATLKL010000000
VERSION     DATLKL010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117452"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..978
                     /locus_tag="VM713_05130"
                     /pseudo
     CDS             1..978
                     /locus_tag="VM713_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
     gene            1146..2549
                     /gene="odhB"
                     /locus_tag="VM713_05135"
     CDS             1146..2549
                     /gene="odhB"
                     /locus_tag="VM713_05135"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HVH83685.1"
                     /translation="MSTEIRVPQLPESVADATLVAWHKQPGDAIRRDENLADLETDKV
                     VLEVPAPSNGVVREIRVKSGTVVTSGQVLAVIDEGDTAATMPVKLSPAAMAAAAAAAA
                     SQAATAQAPGASKAGSGTATSGAAARAAPAADAEAGREGGKLSPSVRRLVEENRLDPA
                     AIPASGRDGRLTKADVVGFLDKKPVPAASAPGTAPAPVPPVAVAAPPAPAAAPARSSA
                     PAARPATPLPSPPPSGPRAEQRVPMTRLRARIAQRLIEAQSTQALLTSFNEVDLTAVQ
                     ELRARYKERFEKQHGVKLGFMSFFVKACIEALKKFPVVNASVDGNDIIYHEYYDIGVA
                     VSTDRGLLVPIVRDADLKSFAQIEKEVAEYARKARDGTIALADLTGGTFTITNGGVFG
                     SLMSTPIVNAPQSAILGMHKIQDRPMVVDGQVAVRPMMYLAVTYDHRIIDGREAVQFL
                     VYVKETLEDPGRILLGI"
     gene            complement(2577..>3915)
                     /locus_tag="VM713_05140"
     CDS             complement(2577..>3915)
                     /locus_tag="VM713_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003123890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HVH83686.1"
                     /translation="THSLPPHVAVVYSGTLNFLGVLASSGAVAFGIISLLPVELILQV
                     GSGAGFSMVFALLIAAILWNLGTWWLGLPASSSHTMIGSIIGVGVANALMHGRDGTSG
                     VDWAQATKIGYSLLLSPLVGFICAALLLLLLKVLVRNRDLYQEPRANTSPPWWIRGIL
                     ILTCGGVSFAHGSNDGQKGMGLIMLILIGTVPMAYALNRAMPARQMAPFIQMAGETQQ
                     ALQQAAPGQAPAPADPRQTLSTYVRTHAWNDEVLPALAAITGSIGEQVKASGTFAQIP
                     GERVTNVRNDMYLASEAIRFYGKDAHSKALSDATRQKLDGFKKQIDLATKFIPLWVKV
                     AVAIALGLGTMVGWKRIVVTIGEKIGKTHLTYAQGACAEVVAMATIGAADMFGLPVST
                     THVLSSGVAGAMAANRSGLQLATLRNLAVAWVLTLPAAILLSGTLYWLFRQIF"
BASE COUNT          651 a         1323 c         1372 g          569 t
ORIGIN      
        1 tacgcgacgc tggtcacgga cggcttcgcc gtacgtctca ccggccagga caccgggcgc
       61 ggcaccttct tccaccgcca cgcggtgctg cacgaccaga acagcgacgc cacctggatc
      121 ccgctgcagc acctcgccgc ggggcagccg cgcgtgcagg tgatcgactc ggtgctgtcc
      181 gaggaagcgg tgctcggctt cgagtacggc tacgcgacga ccgacccggg ctcgctgacg
      241 gtgtgggaag cacagtacgg cgactttgtg aacggcgcgc aggtgatcat cgaccagttc
      301 atcagctcgg gcgaggccaa gtgggagcgc ttctgcggcc tggtgctgct gctgccgcac
      361 ggctacgaag gcgcaggccc tgagcactcc tcggcgcgcc tcgagcgttt cctgcagctg
      421 tgcgccgaga acaatatgca ggtgtgtgtg ccttcgacgc cggcgcagat gttccacatg
      481 ctgagaaggc agatgcgcca ggcgttccgc aagccgctca tcgtcatggc gccgaagagc
      541 ctgctgcgcc acgagctgtc ggtgtcgccg ctcgaggatc tcacccgcgg cagcttcgcg
      601 cgcgtcatcg gcgagatcga tccgctgccg gccgccgagg tgcggcgtgt cgtattctgc
      661 agcggtaagg tgttcttcga tcttctcagg gcgcgccgca aggaagcgct gcgggatgtg
      721 gcgctggtgc gcatcgagca gctctacccg ttcccgagcg aggagtacga ggccaccctt
      781 ggccgcttcg cgaacgcacg cgaggtggtg tggtgccagg aggagccgca gaaccagggg
      841 gcgtggtatc agatccggca ccggctgcag gagctggtgg cgggtcggcg tccagtgctg
      901 tacgccggcc gggccccggc ggccgcgccc gcgaccggca tcgcgcggat ccacgagctc
      961 gagcagcagg cgctggtggc cgcggcgctg catgcgacga ccaccgagga gtcggcgcgc
     1021 gatacgacgc gcctgacggc cgcggtgccg gctgcggtgc cggcagcgga ggcggcggct
     1081 gcggccacca ccgccgaggg acaagccgcg cccccgccgc cggcgaccca gataaggaag
     1141 cgctcatgag caccgagatc cgcgttccgc agcttcccga atccgtcgcc gacgcgacgc
     1201 tggtcgcctg gcacaagcag ccgggcgatg cgatccgccg cgacgagaat ctcgccgacc
     1261 tcgagaccga caaggtcgtg ctggaagtgc cggcacccag caacggcgtg gtgcgcgaga
     1321 tccgcgtaaa gagcggcacc gtcgtgacga gcgggcaggt gctggcggtc atcgacgaag
     1381 gcgacacggc cgcgaccatg ccggtcaagt tgtctccggc cgcgatggcg gccgcggccg
     1441 cagctgccgc ctcgcaggcg gcgacggcgc aagcgccggg agctagcaaa gccggatcgg
     1501 gcaccgcgac ttcgggcgcc gccgccagag ccgcgcccgc cgccgacgcc gaggcgggcc
     1561 gtgagggcgg gaagctcagc ccctcggtgc ggcggctcgt cgaggagaac cgcctcgatc
     1621 cggccgcaat cccggcctca ggccgagatg gccgcctcac gaaggccgac gtcgtggggt
     1681 tcctcgacaa gaaacccgtg ccggcggcga gcgcacccgg cactgcgccg gcgcctgtgc
     1741 cgccagttgc cgttgcagct ccgcccgcac cggcggcggc accggcccgc agcagcgcac
     1801 cggctgcgcg cccggctacg ccgctgccct ctccgccgcc gagcggaccc cgcgccgagc
     1861 agcgcgtgcc gatgacgcgg ttgcgcgcgc gcatcgccca gcgcctcatc gaggcgcagt
     1921 ccacccaggc gctgctcacc tcgttcaatg aagtcgacct gacggcggtg caggagctgc
     1981 gcgcgcgtta caaggagcgc ttcgagaagc agcacggcgt gaagctcggc ttcatgtctt
     2041 tcttcgtaaa ggcctgcatc gaggcgctga agaagttccc ggtggtcaac gcctcggtcg
     2101 acggcaacga catcatctat cacgagtact acgacatcgg cgtggccgtc tcgaccgacc
     2161 gcgggctcct cgtgccgatc gtgcgcgatg cggacctgaa gagcttcgcg cagatcgaga
     2221 aagaggtggc cgagtacgcc cgcaaagcac gcgacggcac gattgcgctc gccgatctca
     2281 ccggcggcac gttcaccatc accaacggcg gcgtgtttgg ctcgctgatg tcgacgccga
     2341 tcgtcaacgc gccgcagagc gccatcctcg gcatgcacaa gatccaggat cgcccgatgg
     2401 tcgtcgacgg gcaggtcgcg gtgcgcccga tgatgtatct cgcggtgacc tacgatcacc
     2461 gcatcatcga tgggcgcgag gcggtgcagt tcctggtgta cgtcaaggaa acactcgagg
     2521 acccggggcg aatcctgctc gggatctgag ggcgcgccgc ggcgcacagc gctgcgtcag
     2581 aagatctggc ggaacagcca gtagagcgtt cccgacagca gtatcgccgc cggcagcgtc
     2641 agcacccagg ccacagccag gttgcgcagc gtcgcgagct gcagcccaga gcggttcgcg
     2701 gccatcgcgc ccgcgactcc cgaagagagt acgtgggtgg tcgagaccgg cagcccgaac
     2761 atgtcggcgg ccccgatggt tgccatcgcg accacttcgg cgcaggcgcc ctgcgcgtag
     2821 gtgagatgag tcttgccgat cttctcgccg atggtcacga cgatgcgctt ccatccgacc
     2881 atggtgccga gtccgagcgc gatggccacc gcgaccttga cccagagcgg aatgaacttc
     2941 gtcgcgaggt cgatctgctt cttgaagccg tcgagcttct ggcgcgtcgc atcgctcagg
     3001 gccttggagt gggcgtcctt gccgtagaag cggatggcct ccgaggccag atacatgtcg
     3061 ttgcgcacgt tggtgacgcg ctcaccgggt atctgcgcga aggtgccgct cgccttgacc
     3121 tgctcgccga tgctgccggt gatggcggcg agggccggca gcacctcatc gttccaggcg
     3181 tgagtgcgca cgtaggtcga gagggtctgg cggggatcgg cgggcgccgg cgcctggcct
     3241 ggtgccgcct gttgcagcgc ctgctgcgtt tcgccggcca tctggatgaa cggggccatc
     3301 tgccgcgccg gcatggcgcg gttcagcgca tacgccatcg gcaccgtgcc aatgaggatc
     3361 agcatgatga ggcccatgcc cttttgcccg tcgtttgagc catgcgcaaa ggagacaccg
     3421 ccgcaggtga ggatcaggat gccgcggatc caccaggggg gtgaggtatt ggcgcgcggc
     3481 tcctggtaga gatcgcggtt tcgcaccagc accttgagca gcagcagcag cagcgccgcg
     3541 cagatgaagc cgacgagcgg cgagagcagc agcgagtagc cgatcttggt cgcctgcgcc
     3601 cagtcgacgc cgctcgtgcc gtcgcggccg tgcatgagtg cgttggccac acccacgccg
     3661 atgatcgagc cgatcatcgt gtgagaggag gaggccggca gtcccagcca ccaggtgccg
     3721 aggttccaca ggatcgccgc gatcagaagc gcgaacacca tcgagaatcc ggcgcctgag
     3781 ccgacctgca ggatcagctc gaccggcagc agcgagatga tgccgaaggc caccgcgccg
     3841 ctcgaggcca gcacgccgag aaaatttagc gtgcccgagt acaccaccgc cacatgcggc
     3901 ggcagggaat gggta
//
LOCUS       DATLKL010000393         1826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_162414, whole genome shotgun sequence.
ACCESSION   DATLKL010000393 DATLKL010000000
VERSION     DATLKL010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162414"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="VM713_05145"
     CDS             complement(<1..1706)
                     /locus_tag="VM713_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVH83687.1"
                     /translation="MYRAPLREMRFVLEELLRAGTLSGCPAFADYSDELGRSVLEEAA
                     RFAQDVLDPLNRPGDTEGARWTPDGVVAAPGFRAAYQQFVAGGWPALGSDPDYGGQAV
                     PRVLVSAVGEFWGSANLAFKLGPMLTHGASRALELTGSAEQKRRFLPKMVSGEWSGTM
                     VLTEAQAGSDLGMVRTRAVPEGDHYRLFGQKIFITWGDHDLTANTIHMVLARIEGAPA
                     GVKGISLFIVPKVLVNADGLLGEPNDVRCLSIEHKLGINASPTCVLAFGEKVGALGYL
                     IGEPGRGLEYMFIMMNSARLAVGSEGYSVGERAFQRAAEWARTRVQGRPPGAERGSAA
                     PIINHPDVKRMLLTMKSQTEASRALTLYTALQFDLAAASADAAVRAAAQARGELLTPI
                     VKGWCTEVGNEVAAIGVQVHGGMGYIEETGAAQYVRDVRITTIYEGTTGIQSNDLIGR
                     KIARDRGAAMSAFLAEMQRELAALAVTDQAAEATRRAALEGVALLVRATQSLGKALAT
                     RPELAQAVSVPYLKLCGYVAGGWLLAKSAALAAAGGADREFYAAKLRTAGFYAAQVLP
                     KAA"
BASE COUNT          271 a          661 c          626 g          268 t
ORIGIN      
        1 gcggccttcg gcagcacctg cgcggcatag aagcccgcgg tgcggagctt ggcggcgtag
       61 aactcgcgat ccgcaccgcc ggccgcggcg agcgcggcgg acttcgcgag caaccacccg
      121 cccgctacgt aaccgcaaag cttgaggtac ggcaccgaca cggcctgcgc cagctcgggg
      181 cgtgtcgcga gcgccttgcc gagggactgc gtggcgcgga cgaggagagc gacgccctca
      241 agcgcggcgc gccgcgtcgc ctcggccgcc tgatccgtca cggcgagcgc tgcgagctcg
      301 cgctgcatct cggcgaggaa tgcgctcatg gcggcgccac ggtcgcgcgc gatcttgcgg
      361 ccgatgagat cgttcgactg gatcccggtc gtgccttcat agatggtggt gatgcgcacg
      421 tcgcgcacgt actgcgccgc gcccgtctcc tcgatgtagc ccatgccgcc atgcacctgc
      481 acgccgatgg cggccacctc gttgccgacc tcggtgcacc agcccttgac gatcggcgtc
      541 agcagctcgc cgcgcgcctg tgcggcggca cgcaccgcgg catcagcgct cgccgcggcg
      601 agatcgaact gcagagcggt gtagagagtg agcgcgcgcg aggcctcggt ctgcgacttc
      661 atcgtgagga gcatgcgctt cacgtcaggg tgattgatga tcggcgcggc gctgccgcgc
      721 tcggcccccg gcgggcggcc ctgcacgcgc gtgcgcgccc actcggccgc ccgctggaag
      781 gcgcgctcgc cgaccgagta gccttcgctg ccgacagcga gccgggcgct gttcatcatg
      841 atgaacatgt actccaggcc ccggccgggc tcgccgatca gatagcccaa cgctccgacc
      901 ttctcgccga aggcgagcac gcaggtcggg ctcgcgttga tgccgagctt gtgctcgatc
      961 gacaggcagc gcacgtcgtt gggctcgccg agcaagccgt cggcgttcac cagcaccttg
     1021 ggcacgatga acagcgagat gcccttcacc cccgcaggcg ctccctcgat gcgcgccagc
     1081 accatgtgga tggtgttggc ggtgagatcg tgatcgcccc aggtgatgaa gatcttctgg
     1141 ccgaagagcc ggtagtggtc cccctcgggt acggcgcgcg tgcgcaccat tcccaaatcc
     1201 gagcccgcct gcgcttcggt gagcaccatg gtgccgctcc actcgccgct caccatcttc
     1261 ggcaggaagc ggcgcttctg ctccgcggag ccggtgagct cgagcgcgcg cgaggcgccg
     1321 tgggtcagca tcgggccgag cttgaaggca agattcgccg agccccagaa ctccccgacc
     1381 gcgctcacca gcacgcgcgg caccgcctgc cccccgtagt caggatcgga gccgagcgcc
     1441 ggccagccgc cggcgacaaa ctgctggtag gcggcacgga accccggcgc cgcgaccacg
     1501 ccatcgggcg tccagcgcgc gccctcggtg tcgcctgggc ggttgagcgg atcgagcaca
     1561 tcctgcgcga agcgcgcggc ttcctcgagc accgagcgac cgagctcatc ggagtagtcg
     1621 gcgaaggccg ggcagccgct gagcgtgccc gccctgagga gctcctcgag cacgaagcgc
     1681 atttccctca gtggtgcacg gtacatggac gaccccctcc tcctctcctg accggccgcg
     1741 caacggcgcc cggcgccttc cttggccgga gaggctttca gtgtaccttg gtcttcaagc
     1801 gtgggcggca gcgggagccg ccagcg
//
LOCUS       DATLKL010000394         1802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_27151, whole genome shotgun sequence.
ACCESSION   DATLKL010000394 DATLKL010000000
VERSION     DATLKL010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27151"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VM713_05150"
     CDS             <1..626
                     /locus_tag="VM713_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HVH83688.1"
                     /translation="LKVAHPWWQFSARFNVAISQSVPVARMHERESEGVMMRWGLAHS
                     TASGTIEFTTCGLVRHDALPSARDLHSMWVHGRRGIVPVAGFYLWQRAAAGHEQPYYV
                     RVVNRPVFGVAVLWERAEANDEVIESCALITVAANALLAEINPAAGQMPAILAAADYD
                     AWLSASAQRANELLQPYPHTRMVSHPVAPYVNHLEFDEPSLIRPADR"
     gene            complement(660..>1802)
                     /locus_tag="VM713_05155"
     CDS             complement(660..>1802)
                     /locus_tag="VM713_05155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HVH83689.1"
                     /translation="ILDAANAVIALAPARYPKELFSTRRSSERPELVTTADEQAEADY
                     VADRVLEHREAGIALQRQAVLMRAAHHSDLLEVELARRNIPFVKYGGLRFLEAAHVKD
                     LLSVLRWVDNPRNTLAAFRVLQLLPGMGPVNASAAIEHMEAAGYAFESLRSFKPPQTH
                     EMDWKRLIELMLTLSDPELQWAGQVHQARTWYQRHFERIYEHFHTRLGDLDQLEHLSG
                     QYPSRERFITELTLDPPHATSDLAGQPALEEDYLVLSTIHSAKGMEWDTVYVLNVVDG
                     SFPSEFATGKVELIEEERRLLYVALTRAQNDLALVMPLKFYLTQQPRQGDAHVYGGRS
                     RFMTEKVLKTLDAVTFHGSLGAGGDALQSTGEQVTVDVGARLREMW"
BASE COUNT          298 a          584 c          605 g          315 t
ORIGIN      
        1 aactcaaggt cgcgcatccc tggtggcaat tttcggcgcg cttcaatgtc gccatctcgc
       61 agagcgtccc ggtagctcgg atgcacgagc gcgaatcgga aggcgtgatg atgcgctggg
      121 gtcttgcgca cagcaccgca agcggcacca tcgaattcac cacgtgcggc ctggtccgcc
      181 acgacgctct gccgtctgca cgagacctgc actccatgtg ggttcacggc cgacgcggca
      241 tcgtgcctgt tgcgggattc tatctctggc aacgcgcggc cgccggccac gagcaaccct
      301 actacgtgcg cgtggtgaac cgaccggtct tcggcgtggc agttctctgg gagcgagcgg
      361 aagccaacga cgaggtaatc gagagttgtg cgctcatcac cgtcgcggcc aatgcgttgc
      421 tcgccgagat caaccctgcg gcgggacaga tgcctgcgat cctcgcagcg gcggactacg
      481 acgcgtggct gtcagcaagc gcgcagcggg cgaacgagct gttgcagccg tatccgcaca
      541 cgcggatggt cagtcaccct gtcgcgccct acgtaaatca cctggagttc gacgagccca
      601 gtctgatcag acctgctgat cggtagcgct gcggtccgcc ccgtacccgg cgagtggtcc
      661 taccacatct ctctcaggcg cgccccgaca tcgaccgtga cctgctcacc ggtgctttgc
      721 agggcgtctc cgcccgcgcc gagcgagccg tggaaagtca cggcatcgag cgtcttcaga
      781 accttctccg tcatgaagcg actgcggccg ccgtagacgt gtgcatcacc ctggcgcggc
      841 tgctgggtga gatagaactt gagcggcatg acgagtgcga ggtcgttctg ggcacgcgtc
      901 agcgcgacgt acagcagccg ccgctcctcc tcgatgagct cgactttccc ggtggcgaac
      961 tccgagggga aactaccgtc gaccacgttg agcacgtaga ccgtatccca ctccatgccc
     1021 ttggctgagt ggatggtgga gagcaccaga taatcctcct cgagcgccgg ctgcccggcg
     1081 agatcgctgg tggcatgcgg cggatcgagc gtgagctcgg tgatgaagcg ctcgcgcgac
     1141 gggtattgcc ccgagaggtg ctcgagctga tcgagatccc cgagccgggt gtggaagtgc
     1201 tcgtagatgc gctcgaagtg ccgctgatac cacgtacgcg cctggtgcac ctggccggcc
     1261 cattgcagct ccgggtcgga gagcgtcagc atcagctcga tgagccgctt ccagtccatt
     1321 tcgtgggtct gcggcggctt gaaggaccgg agcgactcga aggcatagcc ggcggcttcc
     1381 atgtgctcga tcgctgcgct cgcattgacc gggcccatgc ccggcagcag ctgcagtacg
     1441 cggaaggcag cgagggtgtt acgcgggttg tcgacccagc gcagcaccga cagcagatcc
     1501 ttgacgtgcg ccgcctcgag gaacctgagc cccccgtatt tgacgaacgg gatgttgcgc
     1561 cgtgcgagct cgacctcgag caggtcggag tggtgcgcgg cgcgcatcag caccgcctgg
     1621 cgctggagcg cgattcccgc ctcgcggtgc tcgagcaccc ggtcggcgac atagtccgct
     1681 tcggcctgct cgtccgcggt ggtcacgagc tcggggcgct cgctcgagcg ccgcgtggag
     1741 aagagctcct tggggtaccg ggccggggcg agcgcgatca cggcgttcgc ggcgtcgagg
     1801 at
//
LOCUS       DATLKL010000395         2580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_4469, whole genome shotgun sequence.
ACCESSION   DATLKL010000395 DATLKL010000000
VERSION     DATLKL010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4469"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..773)
                     /locus_tag="VM713_05160"
     CDS             complement(219..773)
                     /locus_tag="VM713_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HVH83690.1"
                     /translation="MRRILFTLLSLACSVTLATTIYKWVDENGVIHYSDQPHENAEKL
                     QVEGAQTYSASAPAPRGPAAPAQSSAANTYRGCAIVQPVDQQNLPNAESVMIRVVADP
                     VPRGGDRVYITMDGLGLNGTQPTGSSFNVTSIDRGEHTVQAQIRGNDDAVLCQTPAVT
                     FYVQQHTLFSPAASGQPGVSPRPH"
     gene            complement(971..2383)
                     /gene="glnA"
                     /locus_tag="VM713_05165"
     CDS             complement(971..2383)
                     /gene="glnA"
                     /locus_tag="VM713_05165"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HVH83691.1"
                     /translation="MTTPSDVIRLIKEKQVKFADLRFTDTRGKEQHVTIPARLVDEDF
                     FRDGKMFDGSSIAGWKGINESDMILMPEANTAVIDPFFEETTVDIRCDVIEPATMQGY
                     ERDPRSLAKRAEAYLKSTGIADYALFGPENEFFIFDSVRFGNEMNGAFYSIDSVQGAW
                     NSEREYQEGNKGHRPGIKGGYFPVPPVDAFQDIRSAMCLAMEEMGLKVEVHHHEVATG
                     GQAEIGVGAGTLVQKADQVQILKYCVHNVANGYGKTATFMPKPLVGDNGNGMHVHQSL
                     QKEGRALFAGDKYGGLSDLALYYIGGIIKHAKALNAITNPGTNSYKRLVPGFEAPTLL
                     AYSARNRSASIRIPWVSSPKARRIEVRFPDSCANPYFAFAAMMMAGLDGIQSKIHPGD
                     PSDKDLYDLEPEEMKDIPTVCHSLDMALEQLDRDREFLTRGGVFTNDVLDAYMGLKLQ
                     EITRFRMSTHPVELELYYSC"
BASE COUNT          401 a          815 c          892 g          472 t
ORIGIN      
        1 gcgagcaggt cctgcgccgc cacgttggcg tagatcgggc agagctgcgc atcgagcatc
       61 aggatcccgg tcgagagcgc atcgagcagc tccgccggat cgaagtgcga gagcgccgaa
      121 ggggtgcggt actcagcggc cacggcgccg cctgcttcgg ttcagctgcg ggagcggcgc
      181 gcggcgcgcg ctcgcggcgg ttcgtgcgct tggcgcggtt agtgcgggcg cggcgagacg
      241 cccggctgcc cgctcgctgc cgggctgaag agcgtgtgct gctgcacgta gaaggtcacc
      301 gccggcgtct ggcacagcac cgcatcgtcg ttgccgcgga tctgcgcctg cacggtgtgc
      361 tcgccacgat cgatcgacgt cacattgaag ctggatccgg tcggctgcgt gccgttcagg
      421 cccaggccgt ccatcgtgat gtagacgcgg tcgccgcccc gcggcacggg atcggccacg
      481 acgcggatca tcaccgactc cgcattcggg agattctgct ggtcgaccgg ttgcacgatg
      541 gcgcagcccc ggtaggtatt cgcggctgag ctctgggccg gcgcggcggg cccacgcggc
      601 gcgggcgcac tcgcggaata ggtctgcgcg ccctccacct gcagcttctc cgcgttctcg
      661 tgcggctgat cgctgtagtg gatcacgccg ttctcgtcca cccacttgta tatcgtggtc
      721 gcgagcgtca ccgagcaggc gagggacaat aaggtaaaga ggatgcgccg catcgcagcc
      781 gagcatatcc cggcatgacc gaggagacaa aagcggcacg ccgcgaatgt gcgctgccgc
      841 tcgcgtgcgc gcagtgcgcg ctgaccggcg cactggcggc cgctcccccg gagcgcccgc
      901 cggcccgccc cggaacgggc cggacgctcg cagggcgggg cgcgggggcg gcggtcgcgt
      961 cattccgtga tcagcagctg tagtacagct ctagctccac cgggtgtgtg gacatgcgaa
     1021 agcgcgtgat ctcctggagc ttcaagccca tgtacgcatc gagcacgtcg ttggtgaaga
     1081 cgccgccgcg ggtgaggaac tcgcggtccc tgtcgagctg ctcgagcgcc atgtcgagcg
     1141 agtggcacac ggtcggaatg tccttcatct cctcgggctc gaggtcgtag agatccttgt
     1201 cggaagggtc gcccgggtgg atcttgctct ggatgccatc cagccctgcc atcatcatcg
     1261 ccgcgaaggc gaagtagggg ttggcgcacg agtcggggaa gcgcacctcg atgcgccgcg
     1321 ccttgggcga ggacacccac gggatgcgga tcgaggcgga gcggttgcgc gccgagtagg
     1381 cgagcagcgt cggcgcctcg aagccgggca ccaggcgctt gtagctgttg gtgccggggt
     1441 tggtgatggc gttcagcgcc ttggcgtgct tgatgatgcc gccgatgtag tagagcgcga
     1501 gatccgagag cccgccgtac ttgtcgccgg caaagagcgc gcgcccttcc ttctgcagcg
     1561 actggtgcac gtgcatgccg ttgccgttgt cgcccaccag gggcttcggc atgaaggtcg
     1621 cggtcttgcc gtagccgttg gcgacgttgt gcacgcagta cttgaggatc tgcacctggt
     1681 cggccttctg caccagcgtc cccgcgccga cgccgatctc ggcctggccg ccggtcgcca
     1741 cctcgtggtg atgcacctcg accttgagcc ccatctcctc catcgcgagg cacatcgccg
     1801 agcggatgtc ctggaaggca tcaaccggcg gcaccgggaa gtagccgccc ttgatgccgg
     1861 gacggtggcc cttattgccc tcctggtact cacgctcgga gttccaggcg ccctgtaccg
     1921 agtcgatcga gtagaaggcg ccgttcatct cgttgccgaa acggacgctg tcgaagatga
     1981 agaattcgtt ctccggtccg aagagggcgt agtcggcgat gccggtggac ttgagatagg
     2041 cctcggcgcg cttggcgagc gagcgcggat cgcgctcgta gccctgcatc gtcgccggct
     2101 cgatcacgtc gcagcggatg tcgaccgtcg tctcctcgaa gaacggatcg atgacggcgg
     2161 tgttggcttc gggcatcagg atcatgtccg actcgttgat gcccttccat ccggcgatgc
     2221 tcgagccgtc gaacatcttg ccgtcgcgga agaagtcctc gtcgacgagc cgcgccggga
     2281 tcgtcacgtg ctgttccttg ccacgcgtat cggtgaagcg caggtccgcg aacttcactt
     2341 gtttttcctt gatgagcctg atcacgtcac ttggtgtcgt catatcgcct ccggggagaa
     2401 agggggtcgg gcggccgccg cctcttgagt cctcgagcca ttgcaggccc tgtgccatgg
     2461 acacagggaa gggaaatcaa acggttagcg gacattctga agggtcattg caccattcgg
     2521 gtgcgtccag gagtgattat tgcactccat atgtgcgcat aacaggggca cgctgcattt
//
LOCUS       DATLKL010000396         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_49584, whole genome shotgun sequence.
ACCESSION   DATLKL010000396 DATLKL010000000
VERSION     DATLKL010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49584"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..>2045)
                     /locus_tag="VM713_05170"
     CDS             complement(69..>2045)
                     /locus_tag="VM713_05170"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HVH83692.1"
                     /translation="TRSKNSGAGKVVQSSAERIANANLTRFMRCVMERTGIPLADYEA
                     TYAWSIAQPTQFWTELARFADVRADFGDGPVIEHPTQMPGARFFPAAHLNFAENLLKF
                     ADGREALVFRNERGTRRALTGRELRAEVARVAAGLKAAGVIAGDRIAGYLPNLPEATV
                     AMLATASIGAIWSSCSPDFGVHGVLDRFGQIQPKVLFTADGYFYAGKTLDSLAPMAEV
                     LAQLSHIQRVVVIPYVTAQPELARLGSAAGRASLWAEFGMRGAPLRFAPQPFNHPLYI
                     LYSSGTTGVPKCIVHGAGGTLLQHQKEQLLHTDLGRSDRLFYFTTCGWMMWNWLMSAL
                     ATGATLVLYDGSPFHPDPGVLWRMAAEERVTVFGTSAKYLASLEKSEFVPARAVDLTA
                     LRAVLSTGSPLLPDGFDFVYRSVKSDLQLASISGGTDIVACFALGCPIRPVYRGEIQC
                     RGLGMAVEVFDEEGHPLRGARGELVCTAPFPSMPIQFWNDPDGARYRAAYFERFPGVW
                     HHGDFAAITAHDGVVIYGRSDAVLNPGGVRIGTAEIYGAVEALPEIVESLAVGQDWQG
                     DVRVVLFVRLKPGVELTEELRQRIRLTIRTQTTARHVPARIIAVPDIPRTLSGKPTEL
                     AVRNVIHGLPVKNVDALANPQALEHFRNLPELAT"
BASE COUNT          334 a          674 c          738 g          299 t
ORIGIN      
        1 gccgcggacg gcaagctcgc gtgcatagag ctgccgcagg tcggagaccg cggccacttg
       61 ctcagggctc aggtggcgag ctcgggcagg ttgcggaagt gctcgagcgc ctgcggattg
      121 gcgagcgcgt cgacgttctt caccgggagc ccgtggatga cgttgcggac ggcgagctcg
      181 gtgggcttcc ccgacagggt gcgcgggatg tcgggcacgg cgatgatgcg cgccgggacg
      241 tgccgcgcgg tggtttgcgt gcggatcgtg aggcgaatcc gctgccgcag ctcctcggtg
      301 agctcgacac cgggcttgag gcgcacgaac agtacgacgc gcacgtctcc ctgccagtcc
      361 tggccgaccg ccagcgactc gacgatctcg ggcagtgcct ccaccgcgcc gtagatttcc
      421 gcggtgccga tgcgtacgcc gccgggattc aagaccgcgt ccgaccggcc atagatcacc
      481 acgccatcgt gtgcggtgat cgcggcgaag tccccgtgat gccacacgcc cgggaagcgc
      541 tcgaaatagg cggcgcggta gcgcgcgccg tcggggtcgt tccagaactg gatcggcatc
      601 gaggggaagg gcgcggtgca gaccagctcg ccgcgcgcgc cgcgcagcgg gtggccctcc
      661 tcatcgaaca cctcgaccgc catgccgagc ccgcggcact gaatctcgcc ccggtagacg
      721 gggcggatgg ggcagccgag cgcaaagcac gcaacgatgt cggtgccacc cgagatcgag
      781 gcgagctgca ggtccgactt gacgctgcga tagacgaaat cgaacccgtc gggcaggagc
      841 ggtgagccgg tcgagagcac ggcgcgcaac gccgtgagat ctaccgcgcg cgccggtacg
      901 aactcgctct tctcgagcga ggcgaggtac ttggcgctgg tgccgaacac ggtgacgcgc
      961 tcctcagcgg ccatgcgcca cagcacgccg gggtcgggat ggaagggact gccgtcatag
     1021 agcaccagcg tcgcgccggt cgcgagcgcg ctcatcagcc agttccacat catccagccg
     1081 caggtggtga agtagaagag gcggtcactg cgcccaaggt cggtgtgcag cagctgctcc
     1141 ttctggtgct gcagcagcgt accgccggcg ccgtggacga tgcatttcgg cacgcccgtg
     1201 gtgcctgaag aatagaggat atagagcgga tggttgaagg gctgtggcgc aaaccgcagc
     1261 ggcgccccgc gcatgccgaa ctcggcccag aggctggctc gccccgccgc gctcccgagc
     1321 cgcgcgagct ccggctgcgc cgtcacgtac ggaatgacga ccacgcgctg gatgtggctg
     1381 agctgcgcga gcacttcggc catgggagcg agcgaatcga gcgtcttgcc ggcgtagaag
     1441 tagccgtcgg cggtgaacag caccttgggc tggatctgcc cgaagcggtc gaggacgccg
     1501 tgcacgccga aatccggcga gcacgacgac cagatggcgc cgatgctcgc cgtcgccagc
     1561 atcgcgaccg tcgcctcggg caggttcggc aggtagccgg cgatgcggtc gcccgcgatc
     1621 acgccggccg ccttgaggcc ggcggccacg cgcgccacct cggcgcgcag ctcgcgcccg
     1681 gtaagggcgc ggcgcgtgcc gcgctcgttg cgaaacacca gcgcttccct gccgtcggcg
     1741 aatttgagga gattctcggc gaagttgaga tgtgcggcgg gaaagaaacg cgcccccggc
     1801 atctgcgtcg ggtgctcgat caccggcccg tcgccgaagt cggcacgcac gtccgcgaag
     1861 cgcgcgagct cggtccagaa ctgcgtcggt tgcgcgatcg accacgcgta ggtcgcctcg
     1921 taatcggcga gcggaatgcc ggtgcgctcc atcacgcagc gcatgaagcg tgtcaggttc
     1981 gcgttggcga tgcgttcggc ggatgactgg acaactttgc ccgccccgct gttcttcgag
     2041 cgcgt
//
LOCUS       DATLKL010000397         2441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_27161, whole genome shotgun sequence.
ACCESSION   DATLKL010000397 DATLKL010000000
VERSION     DATLKL010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27161"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VM713_05175"
     CDS             <1..964
                     /locus_tag="VM713_05175"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83693.1"
                     /translation="PAAGPRAPAVTKRDLLRYLARVGRFMLPHLADRPLTMIRMPEGI
                     NGERFFQKHWEQPLPEFVETVTVFSETKDDQHRYLLANNLPTLIWLGQIGTLEFHVWH
                     SRARPGPDTPKASTDYASSLAALQASVLNFPDYLIFDIDPYIYSGKEAKGAEPELNKK
                     GFAVGKRVAFWLRALLREMGLEAVIKTSGKTGLHMFVPIERTVTFDEARHICETVGRH
                     VLRAHPKDITMDWAIEKRTGKIFIDYNMNVRGKTLNVAYSPRGVPGTPVSMPLTWEEL
                     EAAEPMDFTLNNVPARLEKRGDRWHDVLELKQSLTKAFGVKGEK"
     gene            976..1854
                     /locus_tag="VM713_05180"
     CDS             976..1854
                     /locus_tag="VM713_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HVH83694.1"
                     /translation="MAAARSIGSLTVSFGLVAIPVKLFTANQSSSAISFNLLHKADGS
                     RLKQQYICQKDGAVVERDDMVKGYEFAKDQYVRFAPEEIKALEEVGSHAVEISEFVPI
                     ESVDPVYYDKTYYLAPDKGAAKPYALLTEALKQAGRCGVGRWAARGKGYLVILRPIGD
                     VLAMQQLHYAADVRRASEVEVAKTEVKPAELKLAQQLIDAQTAEKFDPDAYKDEVRGR
                     IEAAIKKKVDEGQEISMVEPAPPGEGKVIDLMEALRASLEKSGKGRDQASGLGPRKAP
                     KRVEEPAKVARKAGKR"
     gene            1913..>2441
                     /locus_tag="VM713_05185"
     CDS             1913..>2441
                     /locus_tag="VM713_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83695.1"
                     /translation="MQKFLRLSPAITRGLIRAGFVKPTRGPRRAYRLSFQDLIVLRAA
                     RALIDARVPAKRIRRSLQRLRRELPASLPLSGLAISAVGERVVVRDGETRWQADSGQY
                     LLGLDVTLEDGVLQVVEHKPPAAPAAAEAPQPARDWFAEGLTLESTNPGAAAEAYRQA
                     AQENPHNAAAWINLGR"
BASE COUNT          457 a          803 c          805 g          376 t
ORIGIN      
        1 gcccgcggca ggaccgcgcg ctcccgcagt gaccaagcgg gatctgctgc gctatctcgc
       61 gcgagtcggg cgcttcatgc tgccgcacct cgccgaccgg ccgctcacca tgatccgcat
      121 gccggagggg atcaacggcg agcgcttctt tcaaaagcac tgggagcagc cgctcccgga
      181 gttcgtcgag accgtcacgg tgttctcgga gaccaaggac gaccagcacc gctacctgct
      241 cgccaacaat ctgccgacgc tcatctggct cgggcagatc ggcacgctcg agttccacgt
      301 ctggcattcg cgtgcacgcc cgggccccga tacgcccaag gccagcactg actacgcgag
      361 ctcgctcgcc gcgctccagg cctcggtgct caacttcccc gactatctga tcttcgacat
      421 cgacccctac atctattcgg gcaaggaggc gaagggggcc gagcccgagc tcaacaagaa
      481 gggtttcgcg gtcggcaaac gcgtcgcgtt ctggctgcgc gcgctgcttc gggaaatggg
      541 cctcgaggcc gtcatcaaga cctcgggcaa gaccggtctc cacatgttcg taccgatcga
      601 gcggaccgtg accttcgacg aggcccgcca tatctgcgag accgtcggac gccacgtgct
      661 gcgggcccac cccaaggaca tcactatgga ttgggcgatc gagaagcgca ccggcaagat
      721 cttcatcgac tacaacatga acgtacgggg caagacgctc aacgtcgcct actccccccg
      781 aggggtcccc gggacgccgg tctccatgcc gctcacctgg gaggagctgg aggccgccga
      841 gcccatggac tttaccctca ataatgtgcc ggcccgcttg gaaaagaggg gggaccggtg
      901 gcatgatgta ctcgagctaa aacagagcct tacgaaggct tttggtgtca aaggtgagaa
      961 gtagagggtg acgctatggc cgctgctcgc tcaatagggt ctctgacggt gtccttcgga
     1021 ctggttgcga tcccggtgaa gctcttcacc gcaaaccagt cgtcgagcgc catctctttc
     1081 aacctgctgc ataaggccga cggctcgcgc ctcaagcagc agtacatctg ccagaaagac
     1141 ggtgcagtgg tcgagcgtga cgacatggtc aaaggctacg agttcgccaa ggaccagtac
     1201 gtgcgcttcg cgcccgagga gatcaaggcg ctcgaggaag tcggcagcca cgccgtcgag
     1261 atctccgagt tcgtcccgat cgagtccgtc gatccggtgt actacgacaa gacttactac
     1321 ctggcccccg acaaaggcgc ggccaagccc tatgcgctcc tcaccgaggc gctgaagcag
     1381 gccgggcgct gtggcgtcgg ccgctgggcg gcccgcggca agggctatct cgtcatcctg
     1441 cgcccgatcg gcgacgtgct cgccatgcag cagctgcact acgcggccga cgtgcgtcgt
     1501 gcgagcgaag tcgaggtcgc gaagaccgag gtgaagcccg cggagctgaa gctcgcgcag
     1561 cagctcatcg atgcgcagac ggcggagaaa tttgatccgg acgcctacaa ggacgaggtg
     1621 cgcggccgca tcgaggccgc gatcaagaag aaggtcgacg agggccagga gatcagcatg
     1681 gtcgagccgg ctccgccggg cgaaggcaag gtcatcgatc tcatggaggc gctgcgcgcg
     1741 agcctggaaa agagcggcaa gggacgggat caggcgagcg ggctcggccc gcgcaaagct
     1801 cccaagcgag tcgaggaacc ggctaaagta gcgcgcaagg cgggcaagcg ctgacggccg
     1861 gggaacgacc tttccgcaag agctcaggat gcgttcttac agtgtgcggg acgtgcaaaa
     1921 gttcctgcgg ctgtcccctg cgatcacgcg cgggctgatc cgcgccggat tcgtcaagcc
     1981 gacgcgtggc ccgcgtaggg cctatcgcct gtctttccag gatctgatcg tgctgcgcgc
     2041 ggcgcgtgcg ctgattgacg cccgcgtgcc cgccaagcgc attcgccgtt cgctccagag
     2101 gctgcggcgc gagctcccgg cctcgctgcc gctgtcgggg cttgcgatca gtgcggtggg
     2161 cgagcgcgtg gtggtgcgcg acggcgagac ccgctggcag gcggacagcg gtcaatacct
     2221 cctgggactc gacgtcacgc tcgaggacgg cgtgctgcag gtcgtcgagc acaagccgcc
     2281 ggccgcgccc gcggccgccg aggctccgca acccgcgcgc gactggttcg cagaaggtct
     2341 gaccctcgag agcaccaacc cgggcgcagc tgccgaggca tatcgccagg ccgcgcagga
     2401 gaatccgcac aacgccgccg cgtggatcaa cctgggccgc c
//
LOCUS       DATLKL010000398         2422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_38489, whole genome shotgun sequence.
ACCESSION   DATLKL010000398 DATLKL010000000
VERSION     DATLKL010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38489"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VM713_05190"
     CDS             <1..500
                     /locus_tag="VM713_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVH83696.1"
                     /translation="ATDGVYVRPSETMQLLPAHSQTLRVFALSCPPGTLAWPATLPDN
                     FDARYERRVASVDEAQRRATGPRYFQLLVDKGIGSQLITQFIGHIPKSKAAPHRHLYE
                     EAIIVLSGEGCLWTEDRRAQVRAGDVIFLPRKQLHSLEATAPAGLDVVGVICPGDNPS
                     ITYYD"
     gene            493..1914
                     /locus_tag="VM713_05195"
     CDS             493..1914
                     /locus_tag="VM713_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVH83697.1"
                     /translation="MTETGKFAPISERESGLRRGLGKTQIVMIGLGGAIGTGLFMGSG
                     LAIGYAGPAVILSYALAGFVALVMVFSLSEMAVVHPTAGSFGTYAETYLNPWAGFVVR
                     YTYWIAQVIAIGGEAVAAGLYMGLWFPGVPVWMWACGFCVVLLYFNLRSVSNFGTIES
                     WFALIKVAAIVLFIILGAASLFGAGHLAAGLHNLTGLPGGFLPKGLPGVWMAVIVGIF
                     SFNGIEVIAVTSGEARDPQVAIPAALRSMLLRLFLFYVLAVTIIVTATPWTATGVKVV
                     AQSPFVSVFQHSGIAHAAGVMNFVVISAALSSMNTNIYLCARMLFSLSRGHYAPRKLG
                     DLGRRGTPVAAVLVSGAGILVSAAVSIVTPNAYGYLLGVALFAAILVWIFILLSHLSF
                     RRQHPASELPVRMPLFPWMQLAGLLMLAAILVTMGLDANLYVSWVYGVPWLILISAGY
                     FLWRAHRRRAGPPHAAVRAAPPA"
     gene            1922..>2422
                     /locus_tag="VM713_05200"
     CDS             1922..>2422
                     /locus_tag="VM713_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVH83698.1"
                     /translation="MTDFRALRRRFPVLERKTYLNSGSYCALADSVRDAINAYMDDRL
                     QVGANWDVWVTKNEAVRSAMAAVLRADRDEVAVTASASAGLNALASALDFSGRRNKVV
                     VSDFEFPTNAQIWHAQEPRGARVVHVPKTAGGDIPLESFARMIDEGTQLVAVTHVCFR
                     NGARLDI"
BASE COUNT          349 a          790 c          824 g          459 t
ORIGIN      
        1 aggcaaccga cggcgtttac gtgcgcccgt ccgagacgat gcaattgctg cccgcgcact
       61 cgcagaccct gagagtcttc gcgttgagct gtccgcccgg gacgctcgcg tggcccgcga
      121 cactgcccga caacttcgac gcgaggtacg agcggcgagt ggcctccgtc gatgaggcgc
      181 agcgccgggc gaccgggcca cgctacttcc agctcctcgt ggacaagggc atcggctcgc
      241 agctcatcac gcagttcatc gggcacatcc cgaagtcgaa ggccgcgcca catcgccatc
      301 tctacgagga ggcgatcatc gtgctctccg gcgagggctg cctgtggacc gaggatcgcc
      361 gggcacaggt gcgggcgggc gatgtgatct tcctgccgcg caagcagctg cactctctcg
      421 aggcgaccgc tcccgcgggg ctcgacgtcg tcggcgtgat ctgccccggc gacaatccca
      481 gcatcaccta ctatgactga gacggggaag ttcgccccga tcagcgagcg tgagagcggg
      541 ctgcggcgcg gtctcggcaa gactcaaatc gtcatgatcg ggctcggcgg ggcgattggc
      601 accgggctgt tcatggggag cgggctcgcc atcggctacg cggggccggc cgtgatcctg
      661 agctacgcgc tggcgggatt tgtggcgctg gtgatggttt tcagcctgtc ggaaatggcg
      721 gtggtgcatc ccacggccgg ttcgttcggc acctatgccg aaacctatct caatccctgg
      781 gcaggcttcg tggtgcgcta cacctattgg atcgcgcagg tgatcgccat cggcggcgag
      841 gccgtcgccg cggggctgta catgggattg tggtttcccg gcgtcccggt gtggatgtgg
      901 gcgtgcggct tctgcgtggt gctcctgtat ttcaacctgc gctcggtgag taacttcgga
      961 accatcgaat cctggttcgc gctgatcaag gtcgcggcga tcgtgctgtt catcatcctc
     1021 ggcgcggcaa gcctattcgg cgccgggcac ctcgcggcgg gcctccacaa tctcaccggg
     1081 ctcccgggcg ggttccttcc caaagggctc ccgggcgtgt ggatggcggt aatcgtcggc
     1141 atcttttcct tcaacggcat cgaggtcatc gccgtgacct cgggagaggc gcgcgatccg
     1201 caggtggcta tccctgccgc gctgcgcagc atgctgctcc ggctgttcct gttctatgtg
     1261 ctggccgtga cgatcatcgt gaccgcgacg ccgtggacag ccaccggggt caaggtcgtg
     1321 gcgcagagtc cgttcgtgag cgtgttccag cactcgggga tcgcccacgc cgccggcgtc
     1381 atgaactttg tggtgatcag tgcggcgctc tcgagcatga acaccaacat ctacctgtgc
     1441 gcccgcatgc tgttctcgct gtcacggggg cactacgctc cgcggaagct cggcgacctc
     1501 ggtcgccgcg gcaccccggt cgccgccgtc ctggtctcgg gcgcgggcat cctggtctcg
     1561 gccgccgtct cgatcgtgac gcccaatgct tacggctacc tcctcggcgt cgctctgttt
     1621 gccgcaatcc tcgtgtggat cttcatcctc ctgagccacc tgagcttcag gcgtcagcac
     1681 ccggcgagcg agctgccggt gcggatgccg ctgtttccgt ggatgcagct cgcggggctg
     1741 ctgatgcttg ccgcgatcct cgttaccatg gggctggatg ccaatctcta cgtttcgtgg
     1801 gtgtatgggg tcccatggct gatcctgatt tcggcgggat atttcctgtg gcgggcgcac
     1861 cgccgccggg ccgggccccc gcacgcggca gtacgtgccg cgccaccggc gtgagacgcg
     1921 catgactgac ttccgggcgc tgcggcgccg ctttccggtg ctcgagcgca agacctacct
     1981 gaacagcgga tcctactgcg cgctggctga cagcgtgcgc gacgccatca acgcctacat
     2041 ggacgatcgt ctgcaggtgg gcgcgaactg ggacgtatgg gtgacgaaga acgaggcggt
     2101 gcgctccgcc atggctgcgg tgctgcgggc ggaccgcgac gaggtcgcgg tcactgcctc
     2161 ggcgtccgcc ggactcaatg ccctggcgag cgcgctcgac ttcagcgggc gacgaaacaa
     2221 ggtcgtggtg agtgacttcg agttcccgac caatgcccag atctggcacg cgcaggagcc
     2281 gcgcggcgcg cgcgtcgtgc acgtgccgaa aacggccggc ggcgacatcc cgctcgagag
     2341 ctttgcgagg atgattgacg aggggacgca gctcgtcgct gtgacccacg tgtgcttccg
     2401 taacggtgcg cggctcgaca tc
//
LOCUS       DATLKL010000399         2543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_162462, whole genome shotgun sequence.
ACCESSION   DATLKL010000399 DATLKL010000000
VERSION     DATLKL010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162462"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..2543
                     /gene="uvrA"
                     /locus_tag="VM713_05205"
                     /pseudo
     CDS             112..2543
                     /gene="uvrA"
                     /locus_tag="VM713_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797750.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
BASE COUNT          408 a          901 c          832 g          402 t
ORIGIN      
        1 gagatagtca agagcccgtc gctccggctc tcccgtcctc tattccttgt atagatcccc
       61 atataggggg tttgggatgg gctcgccacc ccacgtacca ttaccagttc gatgcagcaa
      121 attcgcgtgc gcggtgcgcg cactcacaac ctcaagaacc tcaacctcga cctcccgcgc
      181 gagcggttga tcgtggtcac ggggctctcg ggctcgggca agtcctcgct gaccttcgac
      241 accctgtacg ccgaaggaca gcggcgctac gtcgagtcgc tgtcggccta tgcgcggcag
      301 ttcctcgcca ccatggacaa gcccgacgtc gacagcatcg aggggctgtc gccggcgatc
      361 gccatcgagc agaaggcgag ctcacacaac ccgcgctcga ccgtcggcac ggtcaccgag
      421 atctacgatt acctgcggct gctgtacgct cgcgcgggta cgccgcgctg ccccgatcac
      481 ggcatcgact tgagcgcgca gaccgtgagt cagatggtcg accaggtgct gaagcttccc
      541 gagggcacgg cggccgcgct gctcgccccg gtggtcgcgc agcgcaaggg ggagcacctc
      601 gaactgctcg aggacttggg cgcccagggc ttcgtgcgcg tgcgcatcga cggccgcatc
      661 tacgagctcg acgcgctgcc cgcgctcgat ggcagaaaaa agcacgacat cgaggcggtc
      721 gtcgaccgca tgcgcgttcg tcccgacgcc gcgcagcgcc tggcggagtc cttcgagacg
      781 gccctcaggc tctcgggcgg gctcgcgcag ctcatctttc tcgatgagcc cgggcgtgag
      841 ccgctcatct tctcgagccg gcatgcgtgt ccggtgtgcg gctacagcgt gccgccgctc
      901 gagccgaagc tcttctcctt caacaacccc tcgggggcgt gccccgcatg cgacggactc
      961 ggggtcgagg agttcttcga cccgcagcgc gtcgtgctgc atccccacct ctcgctcgcg
     1021 ggcggcgcgg tgcgcggctg ggaccgtcgc aatgagcact acttccacct catccagtcg
     1081 ctcgcgaggc actacgcgtt cgacatcgag actccatgga cggagctccc cgagcacgtg
     1141 caccacgtgc tcctctacgg cagcgggacc gaagccatcg aattcaatga tcgcgcggaa
     1201 ggcggtcggc gcgcgcgccg ccgccatccc ttcgagggga tcctcccgaa tctcacgcgg
     1261 cgctaccgcg agacttcctc gccgatggtg cgcgaggagc tgtcacgcta cctgagcctg
     1321 cgtccctgcc ctgactgcca gggcacacgg ttgaaccgcg ccgcgcgctt cgtcttcgtc
     1381 gcggagcgga gtctcccgga agtggcgcac ctcacggtgg gccgcgcgct cgagtttctg
     1441 cgcggcctcg agctcgcagg ctggcgcggc gaagtggcgg ccaggatcgt caagggcatg
     1501 gtggagcgtc ttgcgttcct cgccgacgtg ggactcgaat acctcacgct cgatcgcagg
     1561 gccgaaacgc tctcgggcgg ggaatcgcag cgcatccgcc tcgcgagcca ggtcggctcg
     1621 gggctcaccg gggtcatgta tatcctcgac gagccctcga tcgggctgca ctagcgcgat
     1681 aaccggcggc tcctcgcgac gctcaaacag ctgcgcgacc tcggcaacac ggtgatcgtg
     1741 gtcgagcacg acgaggaggc gatccgcgaa gccgaccacg tggtggactt gggtcccggc
     1801 gccgctgcgc acggcggcca cctggtcgcg gaggggacgc cacgggagat cgaggcgacg
     1861 cctgcttcga tcaccggcca atacctctcg gggcggcggc gcatcgaggt gccttcctta
     1921 cgcatccggc ccgccccgga ccggcagatc cgcatcgtgg gcgcgcgtgg caacaacctg
     1981 cgcaacgtca cggcggagat tcccctcgga ctcctcacct gcgtcaccgg cgtctccggc
     2041 tcggggaagt cgacgctcat cgtcgatacg ctcttcgggc acacggcggc gcgcttgaac
     2101 ggcgcgcgca tcgaggccgc accctgcgaa cgtatcgagg gcctcgagca gatcgaccgc
     2161 gtcatcgaca tcgatcagag cccgatcgga cgcacgccgc gctcgaaccc ggcgacctac
     2221 acgggactgt tcgcgccgct gcgcgagctg ttcgcggcgg tgccggaagc gcgcgcccgc
     2281 ggctacgacg ccgggcgctt tagcttcaac gtcaaaggcg ggcgctgcga agcctgccag
     2341 ggagacggcg tgctgcgcgt cgagatgcac ttcctgcccg acgtgtacgt accttgcgac
     2401 gtctgcaagg gccaacgcta caaccgcgag acgctcgaga tccgctaccg cggtcgcaac
     2461 atccacgagg tgctggagat gaccgtcgag gaggccgcgc gcttcttcca gaacgtcccg
     2521 gccgccgccg gcaagctgca gac
//
LOCUS       DATLKL010000400         1759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_27195, whole genome shotgun sequence.
ACCESSION   DATLKL010000400 DATLKL010000000
VERSION     DATLKL010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27195"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VM713_05210"
     CDS             <1..292
                     /locus_tag="VM713_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83699.1"
                     /translation="QPVPDFWVASASYGFMQRPDMPDLMRQLAARGCGVVPEKLTYFV
                     GSERVVPRADGGGLPRWMEAIFSALLRNSVHMPDYLNVPRDQLVDLGRQISI"
     gene            356..895
                     /locus_tag="VM713_05215"
     CDS             356..895
                     /locus_tag="VM713_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83700.1"
                     /translation="MSLLRAAAPLWALLSLPLAPSHARADDPCAAFAWDVRHERDLFS
                     KQPVDLASGRAFGDAPALTPDRLYELELRAQPEVSFSAPPSRSWPQEATYAGLARLTL
                     DTAGVYRFSLDQPAWVDVVVNGAVLQSREAQGRIGCSAPYKVVEFALPAGKPLVLEFS
                     ASVTPSIRVSVSRSPVQSP"
     gene            982..>1759
                     /locus_tag="VM713_05220"
     CDS             982..>1759
                     /locus_tag="VM713_05220"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH83701.1"
                     /translation="MFLWLWLATVVSNIGGWLYNAASGWLMTSLDPDPLVVSLVQVAD
                     SLPLFLFALPAGALADMTDKRRFILVLEILTTILCAVFAALVSLHRVNSALLLLFIFL
                     VGTLGAVETPAWQAITPQLVPQASLSSAITLNSIGVNISRVIGPALAGVIILAGGIAA
                     PFWLNAVSNLGVIAVLLWWRPPLRQPRALPPEHLSGAIRAGVRYARYHGSLRATLARA
                     VGFFLFASAYWALLPVLARTQLHGGATLYGVLLATLGAGAV"
BASE COUNT          219 a          631 c          588 g          321 t
ORIGIN      
        1 gcagccggta cctgacttct gggtggcgag cgcctcctac ggcttcatgc agcggccgga
       61 catgccggat ctcatgcgcc agctcgccgc gcgcggctgc ggcgtcgtcc cggagaagct
      121 cacctacttc gtcggctccg agcgcgtcgt gccgcgcgcg gacggcggcg ggctgccgcg
      181 ctggatggaa gcgatcttca gcgccctgct gcgcaactcc gtgcacatgc ccgactacct
      241 gaacgtgccg cgtgatcagc tggtcgacct cgggcggcag atctcgattt aagcccgacc
      301 gcgagcactc gcttataatt gctcgcctga ggggaatggt tccgtgggaa gccggatgag
      361 tcttttgcgc gcagccgcac cgctgtgggc gttgctttcg ctgccgctcg cgcccagcca
      421 cgctcgcgcg gacgatccct gcgccgcctt cgcgtgggac gtgcgccacg agcgcgacct
      481 gttcagcaag cagccggtgg atctcgcgag cggcagggcc ttcggcgatg ccccggcgtt
      541 gacgcccgac cggctctacg agctcgagct gcgcgcgcaa ccggaagtga gcttcagcgc
      601 cccgccgagc cgcagctggc cccaagaagc gacctacgcc ggactcgcgc ggctcacgct
      661 cgacaccgcg ggcgtttacc gcttctcgct cgaccagccg gcctgggtgg acgtggtcgt
      721 caatggcgct gtgctgcagt cgcgcgaggc gcaggggcgc atcggctgca gcgctcccta
      781 caaggtcgtc gagttcgcgc tgccggccgg gaagccgctc gtgctcgaat tcagcgcgag
      841 cgttacgccc tcgatcagag tcagcgtgag ccgctctccg gtccagtcac cgtgaccggc
      901 cacgagtccg cagtcgcgcc gagcggtacg ccggcgccgg cgcctgcgcc tgcgtccgcg
      961 tggagcgcgc tgcaccaccg cgtgttcctc tggttatggc tcgccacggt ggtctcgaat
     1021 atcggcgggt ggctgtacaa cgccgcgtcc ggctggctca tgaccagcct cgatcccgac
     1081 ccgctcgtcg tgtcgctggt ccaggtcgcc gatagcctgc cgctgttcct gttcgccctg
     1141 cccgcgggcg cgctcgccga catgaccgac aagcgacggt tcatcctcgt gctcgagatc
     1201 ctcacgacca tcttgtgcgc ggtatttgcc gcgctggtgt ccctgcaccg ggtaaattcg
     1261 gcgctgctgc tgctgtttat ctttctggtc gggacgctcg gggcagtgga gactccggcg
     1321 tggcaggcga tcacgccaca gctcgtgccg caagcgtcgc tctcctccgc catcacgctc
     1381 aacagcatcg gcgtcaacat cagtcgcgtc atagggccgg cgctggcggg cgtgatcatc
     1441 ctcgctggcg gcatcgcggc acccttctgg ctgaacgcgg tgagcaacct gggagtgatc
     1501 gcggtccttt tgtggtggcg tccgccgctg cgccagccgc gcgccctgcc ccccgagcat
     1561 ctgtcgggcg ccatccgcgc cggggtccgc tatgcgcgct atcacggatc gctgcgcgcg
     1621 acgctcgcgc gtgcggtcgg attctttctg ttcgccagtg cctattgggc cctgctgccg
     1681 gtcctcgcgc gcacgcagct ccacggcggt gccacgctct atggtgtgct gctcgcgacg
     1741 ctcggcgccg gcgcggttg
//
LOCUS       DATLKL010000401         2038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_38506, whole genome shotgun sequence.
ACCESSION   DATLKL010000401 DATLKL010000000
VERSION     DATLKL010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38506"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1188
                     /locus_tag="VM713_05225"
     CDS             37..1188
                     /locus_tag="VM713_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83702.1"
                     /translation="MNLTGLLRDRGEDWPYTAGELPVVFGAPANPTHPLHRRIAYGAT
                     AILVGVTGALGNALVFVNLPQLQGALGLYASEIAWLPTAYVMAIVPMNLLLVKFRAQF
                     GLRLFVLGTTSAFTLVMVAHLFVHSFASSVAVRAASGIAGAALITQSIFYMAQALPLH
                     RRLQGIVIGLGIPQFAVPLARCLFSSELLALGEWRALYLCELGLTLLSLGAVLLITLP
                     PAPRRATYEWTDLLTFAVFASGAAAVSAVVGLGRYLWWHDTPWLGYTLAASIPLLIAA
                     FLIERRRANPLFAMDLLGSAPFLRFIVLSLLLQVAVAEQRVGAVGLLNSAGLNNDQLH
                     PLFWLVTLAMLLGLLTTSFLLRPAWFAQLVALGFLIIAAAAMLDSGVTT"
     gene            1209..1691
                     /locus_tag="VM713_05230"
     CDS             1209..1691
                     /locus_tag="VM713_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83703.1"
                     /translation="MSQFLVGFATTFTLGPLFLYGLGLALPKGPAAFISFLAAYSTTA
                     NLGSLGAFSLVSSYETLREKAHSHQLVEYVVSSHPPVVTRLTEASHLYSAVTADPTLR
                     ATAGAALLDDEITRESSVLAYIDVFQAIALLAALTAAFLLALILWRRYRASRAAALSA
                     "
     gene            1688..>2038
                     /locus_tag="VM713_05235"
     CDS             1688..>2038
                     /locus_tag="VM713_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HVH83704.1"
                     /translation="MSTPAATPTAAAQRDSHWHPPPHSRLGLAIITVIGLTGFSCVLA
                     AWHLWPFTGALEHTEDAYVRGYTTVLSSQVDGYVARVWVRDFDFVQAGQVLVTIDDRT
                     YLARVREAQAALDGA"
BASE COUNT          271 a          742 c          606 g          419 t
ORIGIN      
        1 agctgacccc ggcctcatca gtggcgagac gctcggatga acctcaccgg actgcttcgg
       61 gatcgaggcg aggactggcc ctacacggcc ggtgaactac ccgtcgtctt cggcgcgccc
      121 gccaacccca cacatccttt gcaccggcgc atcgcctacg gcgcaacggc catcctggtg
      181 ggcgtcacgg gcgcactcgg gaacgcgctg gtcttcgtca acctgcccca actgcaaggg
      241 gcgctcggtc tctatgcgtc cgagatcgcc tggctcccca ccgcctacgt catggcgatc
      301 gtcccgatga atctgctcct ggtgaagttc cgggctcagt tcggtctacg cctcttcgtg
      361 ctcggcacca cgagcgcctt cacgctggtc atggtggcgc atctgttcgt gcactcgttc
      421 gcgtcgagcg tcgcagtgcg agcggcgagt gggatagccg gcgcggcgct catcacgcag
      481 tccatcttct acatggcaca ggcgctcccg ctccatcggc gtctgcaagg aatcgtcatt
      541 ggcctcggta tcccccagtt cgcggtgccg ctggcccgat gtctcttctc gagcgaactc
      601 ctggcgctcg gcgagtggcg cgcgctctac ctgtgcgagc tcggcctcac cctgctgtcc
      661 ctgggcgcgg tcctcctcat taccctgccg ccggcgcctc ggcgggcaac ctacgagtgg
      721 acggacctgc tcaccttcgc cgtcttcgcg agcggtgccg ccgccgtgtc ggccgtcgtg
      781 ggactcggtc gctatctctg gtggcacgac acgccgtggc tcgggtacac gctcgcggct
      841 tccatcccgc tcctcatcgc ggcatttctc atcgaacgtc gccgcgcgaa cccgctcttt
      901 gccatggatc tcctcggcag tgcgcccttt cttcgtttca tcgtcctgtc gctcctcttg
      961 caggtcgccg tggccgagca gagggtcggc gcggtgggac tcctgaattc ggcgggcctc
     1021 aacaacgatc agctgcaccc gctcttctgg ctggtcaccc tggcgatgct ccttggcctg
     1081 ctcacgacct cgttcttgtt gcggcccgcc tggttcgcgc agctggtggc gctcgggttc
     1141 ctgatcatcg ccgcggccgc catgctcgat tccggagtga caacctgact cggcccgagc
     1201 agctctacat gagtcagttt ctcgtcggct ttgccaccac ctttacgctc ggaccgctct
     1261 ttctctacgg gttaggcctg gcgcttccca aaggcccggc cgccttcatc tcgttcctgg
     1321 cggcctacag cacgacggcg aacctcggaa gcctcggggc tttctcgctc gtgagttcct
     1381 acgagaccct gcgcgagaag gctcattcac accagctcgt cgagtacgtc gtttcatcgc
     1441 accctcccgt cgtcacccgg ctgacggaag cgagccacct gtacagcgct gtgacggcag
     1501 accccaccct tcgcgcgacc gcaggtgccg ctctcctcga cgatgagatc acccgtgaat
     1561 cgagcgtcct ggcctacatc gatgtctttc aggccattgc actcctcgcc gcgctgacgg
     1621 cggcgttcct tctggccctt atcctgtggc gtcggtaccg cgcatcgcgc gcggcggcgc
     1681 tctccgcatg agtacccccg ccgcgactcc gaccgccgcc gcccagaggg actcgcactg
     1741 gcatccgccg ccgcattcgc ggctcgggct cgcgatcatc accgtgattg gattgacggg
     1801 attttcctgt gtgctcgcgg cctggcacct atggccgttc accggcgcgc ttgagcacac
     1861 ggaagacgcc tacgtccgcg gctataccac ggtcctcagt tcccaggtag acggctacgt
     1921 cgcgcgtgtt tgggtgcggg acttcgactt cgtccaggcc ggccaggtgc tcgtcacgat
     1981 tgacgaccgc acctacctgg ccagggtgcg cgaagcgcag gccgctctgg atggggcg
//
LOCUS       DATLKL010000402         2295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_61024, whole genome shotgun sequence.
ACCESSION   DATLKL010000402 DATLKL010000000
VERSION     DATLKL010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61024"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VM713_05240"
     CDS             complement(<1..1068)
                     /locus_tag="VM713_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVH83705.1"
                     /translation="MSIYDEFLDRNAANYVALSPVSFVERSAEVYGDLPAVVHGGRRY
                     RWRETHERSARLAAALRALGVERGTTVSAMLANTPEMIEAHYAVPALDAVIHTLNTRL
                     DAPLLAWQMHHCEAAVLLTDREFSPVMARALRILRTEHGRTPVVIDVCDSEYAGPGER
                     LGALEYEALLAAHAPLALLKGPANEWDAIAVSYTSGTTGNPKGVVTHHRGAYLNAVSN
                     ALCWEMPQFPRYLWTLPLFHCNGWCFPWTVAMLAGMQVCLRRVEARAILEAMRIHGVD
                     HYCGAPIVHTMLINADPAWRAGITQRVRALVAGAAPPVAMIEGMQRIGFDLTHVYGLT
                     ETYGPAAVAAKRAAWSGESVAE"
     gene            complement(1075..1779)
                     /locus_tag="VM713_05245"
     CDS             complement(1075..1779)
                     /locus_tag="VM713_05245"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HVH83706.1"
                     /translation="MPKSSGEPRVVVTKNGPYLVSGSVPMARQTIVADQEGGSQQWRE
                     SHPFPPQESYALCRCGHSGNKPFCDGTHKKIGFEGTETADRRPYREQAQLMEGPALAL
                     SDAESLCAFARFCDPNGQVWNQVERTDQAEVRATFVRQVNYCPSGRLVAWDRATGKPV
                     EHPLPVSIGVVEDPPEGVSGPLWLRGGIPVIAADGFAYEVRNRVTLCRCGASKHKPFC
                     DGSHAAIKFRDSGARG"
     gene            1939..>2295
                     /locus_tag="VM713_05250"
     CDS             1939..>2295
                     /locus_tag="VM713_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83707.1"
                     /translation="MAKKTHPVPPIPRVGKHHYARELRRLQIELVKLHRHVIARGRRV
                     LVILEGRDAAGKDGTIKRIAAHLSPREVRVVAPGKPSDREETEWYFQRLVGRLPAARE
                     IVLFNRSWYNRAGVEYV"
BASE COUNT          372 a          789 c          795 g          339 t
ORIGIN      
        1 ctccgcgacg ctctcgccgg accaggcggc gcgcttggct gcgaccgccg cgggcccgta
       61 ggtctcggtg agcccgtaga cgtgcgtcag atcgaagccg atgcgctgca tgccctcgat
      121 catggcgacc ggcggcgccg ctcccgcgac cagcgcccgc acccgctgcg tgattcccgc
      181 acgccaggcg ggatcggcgt tgatcagcat ggtatgcacg atgggcgcac cgcagtagtg
      241 atcgacgccg tgaatgcgca tcgcctcgag gatcgcgcgc gcctccaccc tgcgcaggca
      301 cacctgcatg ccggcaagca tcgccacggt ccaggggaaa caccagccgt tgcagtgaaa
      361 cagcggcagc gtccacaggt agcgcggaaa ctgcggcatc tcccagcaca aggcgttcga
      421 tacggcgttg agataagccc cgcggtgatg ggtcaccacg cccttggggt tgcccgtggt
      481 gccggaggtg tacgagaccg cgatcgcgtc ccattcgttc gccggcccct taagaagcgc
      541 gagcggggcg tgtgcggcga gcagcgcctc gtactcgagc gcaccgaggc gctcccccgg
      601 tcccgcatac tcgctgtcgc agacgtcgat caccacgggc gtgcggccgt gctccgtgcg
      661 cagaatgcgc agggcgcgtg ccatgacggg ggagaattcg cggtcggtga gcagcaccgc
      721 cgcctcgcag tggtgcatct gccaggcgag gagcggcgca tcgaggcgcg tgttgagcgt
      781 atggatcacg gcatcgaggg cgggcactgc gtaatgcgcc tcgatcatct ccggcgtgtt
      841 ggcgagcatg gcgctcacgg tcgtgccgcg ctcgacgccg agcgcgcgca gagcggcggc
      901 gagccgtgca ctgcgctcgt gggtctcgcg ccagcggtag cggcggccac cgtgcacgac
      961 ggccggcagg tcgccgtaca cttcggcgct gcgctcgacg aaactcaccg gcgacaacgc
     1021 cacgtagttg gcggcgttgc ggtcgaggaa ctcgtcgtag atgctcatcg atcctcagcc
     1081 gcgtgcgccc gaatcgcgga acttgatggc ggcatgcgag ccgtcgcaga agggcttgtg
     1141 cttggaggcg ccacaacggc acagggtcac acggttgcgc acttcgtagg caaagccgtc
     1201 ggccgcgatc accgggatcc cgccgcgcag ccacagtggc ccgctcacgc cctcgggcgg
     1261 atcctcgacc acgccgatcg acaccggcag aggatgctcg accggcttgc cggtggcccg
     1321 gtcccaggcg accagccgtc ccgaggggca gtaattcacc tgccgcacga aggtcgcgcg
     1381 tacctcggcc tggtcggtac gctcgacctg gttccacacc tgcccgttcg ggtcgcagaa
     1441 gcgcgcaaac gcgcagagcg actccgcgtc cgagagggcc agcgccggtc cctccatgag
     1501 ctgcgcctgc tcccgatagg ggcggcggtc ggcggtctcg gtgccctcga agccgatctt
     1561 cttgtgggta ccgtcgcaga agggcttgtt gcccgagtgg ccgcagcggc acagcgcgta
     1621 gctctcctgg ggcgggaacg ggtgactctc ccgccattgc tgcgagccgc cctcctggtc
     1681 ggcgacgatc gtctgccggg ccatcgggac cgagccgctc accagatagg gcccgttctt
     1741 cgtgacgacc acccgtggct cgccgctgga cttcggcatg aatgactccc gaacgcgctg
     1801 ttgttggatc tgccgcggtg cggagtgcct agactgaacc aagcgcgtgc gcgccacaag
     1861 agtatggccg gtacgcaacg tccgggggaa taattgcaca cggcacctcc agcgagggca
     1921 gctgcagcgg agcagatcat ggcgaagaag acccacccgg taccgcccat ccccagggtc
     1981 ggcaaacatc actacgcgcg cgagctgcgc cggctgcaga tcgagctggt gaagctccat
     2041 cgccacgtca tcgcccgggg acgcagggtg ctggtgatac tcgaggggcg cgacgcggcc
     2101 ggcaaggacg gcaccatcaa gcgcatcgcc gcgcacctca gcccgcgcga ggtgcgggta
     2161 gtggcgcctg gcaagccgag cgaccgcgag gagaccgagt ggtacttcca gcgcctcgtc
     2221 ggccgcctcc cggccgcgcg cgagatcgtg ctgttcaacc gcagctggta taaccgcgcg
     2281 ggcgtcgaat acgtc
//
LOCUS       DATLKL010000403         1809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_61025, whole genome shotgun sequence.
ACCESSION   DATLKL010000403 DATLKL010000000
VERSION     DATLKL010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61025"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VM713_05255"
     CDS             complement(<1..884)
                     /locus_tag="VM713_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83708.1"
                     /translation="MRALFLHPPSFDGYDGGAGARYQMKREVRSFWYPTWLAQPAALV
                     ADSKLIDAPPHGLTFADIAPELKGRDLVVMHTSTPSFKADAKTAQMIREVNPQIRIGL
                     IGAQVAVEPEKSLLAAPAVDFVARNEFDFTIKEVAEDRPWSEIDGLSYRDATGAIVHN
                     PDREPVGNMDELGWVTPVYKRDLRIESYYSGYLQHPYLSFYTGRGCKSRCTFCLWPQT
                     IGGHRYRVRSVADVVEETRWAQRAFPQVKEFFFDDDTLTDNHDRVEELARALGPLGVT
                     WSCNAKANVPRKTLEVMKA"
     gene            complement(898..>1809)
                     /gene="hpnI"
                     /locus_tag="VM713_05260"
     CDS             complement(898..>1809)
                     /gene="hpnI"
                     /locus_tag="VM713_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HVH83709.1"
                     /translation="IICGVRDPHDPALDVVRRLQREFPQLDLKIVADPTQHGSSGKIS
                     NLVNMMALASHDYLVIADSDVRVPADYLGRVVLPLADPGVGIVTCPYRACPRPGLWSL
                     LGSLFVNEWFMPAVRVGSLLGSQSYASGVTIALRRDTLTRIGGFGALAEQLPDDYRLG
                     QLTRQLGLRTVLSELEVETSIGEGSLAELARHELRWLRTIRTVQPLGYAFSGVTFALP
                     VALLAALLAGGNGLTLALLAITAGARLMVNSAPRSSHSLLAQLWLVTLNDLLAFALWC
                     WSFTAREVQWGRVRYRVARDGSAREIG"
BASE COUNT          324 a          575 c          630 g          280 t
ORIGIN      
        1 gccttcatca cttcgagcgt cttgcgcggc acgttcgcct tggcattgca cgaccaggtg
       61 acgccgagcg gcccgagcgc acgcgccaac tcctcgacgc gatcatggtt gtcggtgagc
      121 gtgtcgtcgt cgaagaagaa ctccttcacc tgcggaaagg cgcgctgcgc ccagcgggtc
      181 tcctcgacca cgtccgcgac cgagcgcacg cgatagcgat ggccgccgat ggtctgcggc
      241 cagaggcaaa aggtgcaacg cgatttgcag ccgcggccgg tgtagaagga gaggtaagga
      301 tgctgcagat agccgctgta atagctctcg atccgcaggt cgcgcttgta caccggcgtc
      361 acccagccga gctcgtccat gttgccgacg ggctcgcgat cggggttgtg aacgatggcg
      421 ccggtggcat cacggtagga aagcccgtcg atctcgctcc agggccggtc ctcggcgacc
      481 tccttgatcg tgaaatcgaa ctcgttgcgg gcgacgaagt cgacggcggg cgcagcaagc
      541 aggctcttct cgggctcgac cgccacctgc gctccgatca ggccgatgcg gatctgcggg
      601 ttgacctccc ggatcatctg cgccgtcttc gcatctgcct tgaaggaggg ggtcgaggtg
      661 tgcatcacga ccaggtcgcg gcccttgagc tccggtgcga tgtcggcgaa ggtgaggccg
      721 tgcggcgggg catcgatgag cttcgagtcc gctaccagcg ccgccggctg cgcgagccag
      781 gtcggatacc agaaggagcg cacctcgcgc ttcatctgat agcgggcgcc ggcaccgccg
      841 tcgtagccat cgaaggaggg cggatgcagg aacagcgctc tcactgccgt cagcccctca
      901 accgatctcg cgcgcgctgc cgtcgcgcgc tacccggtag cgcacgcgcc cccactgcac
      961 ctcgcgggcg gtgaagctcc agcaccagag tgcgaacgcg agcagatcgt tgagcgttac
     1021 cagccatagt tgcgcgagga gagagtggga gctgcgagga gcggaattta ccatgaggcg
     1081 cgcgccggcc gtgattgcga ggagcgcgag cgtcagaccg ttcccgccgg cgagcagcgc
     1141 tgcgagaagc gctacgggca aggcgaaggt cacgcctgaa aaggcgtacc cgaggggttg
     1201 caccgtgcga atggtgcgca gccaccgcag ctcgtgccgg gcgagttccg cgagactgcc
     1261 ctcaccgatc gaggtctcga cctcgagctc ggagagcacg gtgcgcagac ccaactgtcg
     1321 cgtcagctgg ccgagccggt agtcgtcggg caactgctcg gcgagcgcgc cgaatccgcc
     1381 gatgcgcgtg agggtgtcgc gccgcagcgc gatggtgaca ccggaggcgt acgactgcga
     1441 gccaaggagt gacccgaccc gcaccgcggg catgaaccac tcattgacga agagcgaccc
     1501 gaggagggac cacaatcccg ggcgcggaca ggcgcggtag ggacaggtca cgatgccgac
     1561 ccctggatcc gcaagaggca ggacgaccct cccgaggtag tcggcaggca cgcgcacgtc
     1621 gctgtcagcg atcaccaggt agtcgtggct ggcgagcgcc atcatgttca cgaggttgct
     1681 gatcttgccg ctgctgccgt gctgagtggg gtccgcgacg atcttaaggt cgagctgcgg
     1741 aaactcgcgt tgtagtcgtc ggactacatc caacgcgggg tcgtggggat cgcgcacacc
     1801 gcagatgat
//
LOCUS       DATLKL010000404         3202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_128828, whole genome shotgun sequence.
ACCESSION   DATLKL010000404 DATLKL010000000
VERSION     DATLKL010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128828"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..525)
                     /locus_tag="VM713_05265"
     CDS             complement(124..525)
                     /locus_tag="VM713_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVH83710.1"
                     /translation="MPAAMPIGEVARAAGIAPSAIRYYEKAGLLPKPGRQSGQRRYGA
                     ETGARLKIIQLAREAGFTIAETRTFLSGFSPSTTPAARWRALAERKLAELEAQSQRIG
                     RMKRLLESSFHCGCLRIEECERAIAGARDGR"
     gene            613..1371
                     /locus_tag="VM713_05270"
     CDS             613..1371
                     /locus_tag="VM713_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83711.1"
                     /translation="MNSSLQLLAALLCALTSAPTPSASNPAVSAALADPARPPEEVQL
                     DALRKPAEVIAFAGLKSGDRVADFMSGGGYFTRIFSGVVGPKGRVYAFIPAQQLANCS
                     ASETAGTKALERDKRYANVEVLIDAADRFAVPEPVDLVWTAQNYHDLHDSFMKPMDIA
                     AMNAAIYRALKPGGVYLVIDHAAEAGSGLRDTETLHRIDPQSIRAEVTAAGFRFEGES
                     AVLRNPDDSHKLRVFDPAIRHRTDQMVLKFRKPG"
     gene            complement(1399..2100)
                     /locus_tag="VM713_05275"
     CDS             complement(1399..2100)
                     /locus_tag="VM713_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVH83712.1"
                     /translation="MTTVTLLAGLTGAPRASAEEPEVERLRTDWAQLEQYRDANRRLP
                     PPGAARPRVIFLGDSLTQSWDLAGLGLDQVELLNRGISGQTSPQMLVRFRQDVVDLRP
                     AVVHILAGTNDLAGNTGPTTLEAIEDNVSSMAEIAAANHIRVVLGSVLPALDYPWRRG
                     LEPAPRIVALNGWMRAYARRRKLVYADYYSALVDSRGGFKSALADDGVHPNRAGYAAM
                     EPIARESLRQALAPH"
     gene            complement(2117..2593)
                     /locus_tag="VM713_05280"
     CDS             complement(2117..2593)
                     /locus_tag="VM713_05280"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVH83713.1"
                     /translation="MQYRYGIVPAAEAARLSGRELLQGMIDGRFPSPPIAEVLSLWLA
                     EVGEGTAVFEGEPGGQFLNPMGIVHGGWTMTLIDSAAGCAAHTLLAAGVGYTTVETKV
                     NFSRAIRTDTGRVRCEGRVINQGRQVISAEALVKNRDGRVLAHGTSTLLVLQDAAK"
     gene            complement(2612..3082)
                     /locus_tag="VM713_05285"
     CDS             complement(2612..3082)
                     /locus_tag="VM713_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HVH83714.1"
                     /translation="MTERLNEIYSLLIPLVDGRLIVPRACVAEVIGFQTPSEMTGAPP
                     WYIGTVPWNGKSVPLVSFEGTCGQMIPPASGRTRVVILHALGTRVEGGNFALVAQGFP
                     QLVRVSSDVVRPDNSRVFPDRAPIICQIRMVNETPVVPDLERLEEMIADETSIG"
BASE COUNT          501 a         1108 c         1080 g          513 t
ORIGIN      
        1 gccaggagaa gcgcggccgc tttgaccatg ctgcgatact gaaccatgtg actgcccctc
       61 atgaagggag ctccccgcgg cagccgttcc cggccggcgg gtagcggagc gcgctgctcg
      121 gtctcaacga ccgtcgcgtg cgcccgcgat cgcccgctcg cattcctcga tccgcagaca
      181 cccgcagtgg aagctcgact cgagcagcct cttcatgcgc ccgatccgct gcgactgcgc
      241 ctcgagctcg gccagcttgc gctcggccag cgctcgccag cgcgccgcgg gtgtcgtcga
      301 cggcgagaac ccggaaagga aggtgcgtgt ctcggcgatc gtgaagccgg cttcgcgcgc
      361 cagctgaatg atcttgagcc gtgcgccagt ctccgcgccg tagcgccgct ggccggactg
      421 ccggccgggc ttgggcagga ggcccgcctt ctcgtaatag cggatggcgg acggtgctat
      481 gccggccgcc cgagccactt ctccaatcgg catcgctgca ggcatcgcgc gctcccgttc
      541 ccgactgctt gacttaaagt gcactttaac tcgtatgctc ggatcgtcac aagctcaact
      601 cgaggcagtc atatgaactc atcattgcag ctgctcgcag ccctcctgtg cgcgctgacg
      661 tcggccccga ccccgtcagc ctcgaacccc gcggtgtccg cggccctcgc cgacccggcg
      721 cgcccgccgg aggaggttca gctcgatgca ctgcgcaagc ccgccgaggt gattgcgttc
      781 gcgggactga agagcggcga ccgtgtcgcc gacttcatgt ccggcggcgg ctatttcacc
      841 cgcatcttca gcggggtcgt cgggcccaag ggtcgcgtgt acgccttcat tcccgcccaa
      901 cagctcgcca actgctcggc gagcgagacc gccggcacca aggcgctcga gcgcgataag
      961 cgctatgcga acgtcgaggt gctgatcgac gccgcggatc gcttcgcggt gccggagccc
     1021 gtcgacctgg tgtggaccgc acagaactac cacgacctgc acgactcgtt catgaagccg
     1081 atggatatcg cggccatgaa tgcggcgatc taccgcgcgc tgaagccggg cggcgtctac
     1141 ctcgtcatcg atcatgcggc ggaagccggc tcggggctgc gtgacaccga gacgctccac
     1201 cgcatcgatc cgcagagcat ccgcgcggag gtgaccgcgg cggggttccg gttcgagggc
     1261 gaaagcgcgg tgctgcgcaa cccggacgac tcgcacaagc tgcgggtctt tgatccggcg
     1321 atccgccacc gcacggatca gatggtcctc aaattccgca agcccggatg aggccggcgc
     1381 gcccgcatcg gtctgtgctc aatgcggcgc gagggcctgg cggagcgact cacgcgcgat
     1441 cggctccatg gcggcgtagc ccgcgcggtt gggatgcacg ccgtcgtcgg ccagcgcgga
     1501 cttgaagccc cctcgcgagt cgacgagcgc ggagtagtag tcggcgtaaa cgagtttgcg
     1561 ccgcctcgca taggcgcgca tccagccatt cagcgccacg atcctcggcg ccggttcgag
     1621 cccccgccgc cacggataat cgagtgccgg gagcaccgag ccgagcacga cccggatatg
     1681 gttggccgct gcgatctcgg ccatggagct tacgttgtct tcgatggcct cgagggtcgt
     1741 gggcccggtg tttccggcca ggtcattggt gccggcgagg atgtggacca cggcgggcct
     1801 gaggtcgacg acatcctggc ggaacctcac gagcatctgc ggtgaggtct ggccgctgat
     1861 accgcgattg agcagctcga cctgatcgag gccgagaccg gccagatccc aactctgagt
     1921 cagggagtca ccgaggaaaa tcacccgcgg ccttgcggcg ccgggcggag gaagccggcg
     1981 gttcgcgtcg cggtattgct cgagctgcgc ccagtcggtt cgcagccgct cgacctccgg
     2041 ctcctcggcg gaggcgcgcg gcgctccggt cagacccgcg aggagcgtga cagtggtcaa
     2101 cactccaagc cggccgctac ttcgccgcat cctgcagcac cagcagcgtc gaggtcccgt
     2161 gggcgagcac ccgcccgtcc cggttcttca ccagcgcctc ggcggaaatg acctggcgtc
     2221 cctggttgat gacgcggcct tcgcaacgca cgcggccggt atcggtgcgg atggcgcgcg
     2281 agaagttcac cttggtctcg acggtcgtgt agccgactcc tgccgcgagc aatgtgtgcg
     2341 ccgcgcagcc ggcggcgctg tcgatgagcg tcatggtcca gccgccgtgg acaatgccca
     2401 tggggttgag gaactgtccg cccggctcgc cctcgaacac cgcggttccc tcgccgacct
     2461 cggcgagcca gagactgaga acctcggcga tgggcggcga cggaaaacgt ccgtcgatca
     2521 tgccctgcag cagctcgcgt ccgctcaacc ttgcggcctc cgccgcgggc acaatcccgt
     2581 accggtattg cacggcggtc aggcttgccg cctacccgat gctggtctcg tccgcgatca
     2641 tctcctcgag gcgctcgagg tccggaacga ccggcgtctc gttcaccatg cggatctggc
     2701 agatgatcgg cgcacggtcg ggaaacactc gcgagttgtc ggggcgcacc acgtccgagc
     2761 tcacgcgcac cagctgcggg aagccctgcg ccaccagcgc gaagttgccg ccttcgacgc
     2821 gcgtgccaag cgcatgcagg atcacgacac gggtgcggcc gctcgcgggt gggatcatct
     2881 ggccacaggt gccctcgaag gacacgagcg gcaccgattt gccgttccag ggcaccgtgc
     2941 cgatgtacca gggtggcgcg ccggtcatct cggacggcgt ctgaaagcca atcacctccg
     3001 cgacgcaggc gcgcggaacg atgagccgcc cgtcgaccag tgggatcagc aggctgtaga
     3061 tctcgttgag gcgctcggtc atggcggcgg gtcgtggggc tcagctgccg gaggcggtct
     3121 ggcgcaccgc ggcgcggcgc cgctcgacca gcggcgcgat cgcgtccagc agctgcgcct
     3181 cctggtatgg cttgccgagg ta
//
LOCUS       DATLKL010000405         2384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_106126, whole genome shotgun sequence.
ACCESSION   DATLKL010000405 DATLKL010000000
VERSION     DATLKL010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106126"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /gene="tsaB"
                     /locus_tag="VM713_05290"
     CDS             <1..595
                     /gene="tsaB"
                     /locus_tag="VM713_05290"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HVH83715.1"
                     /translation="ESILGMIDEVLAEAGASLAGLTAIAFGRGPGSFTGVRLAASVTQ
                     GLAHGGGVPVVPVSDLAAVAQRILADDTGAQRVLVCNDARMREVYWGCFGRSADGLAA
                     PVGEELVSDPGLVALPADWSRGPGLRGAGTGFAAYPELRAAWSSVLAAITEVRPRAAE
                     IALLAVEEVRAGRLRRPEEALPVYLRDNAARPAASGH"
     gene            684..1379
                     /gene="phoB"
                     /locus_tag="VM713_05295"
     CDS             684..1379
                     /gene="phoB"
                     /locus_tag="VM713_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HVH83716.1"
                     /translation="MPAKQVLIVEDERPIREMIAFGLKRAGFEVREAEDCRAARAALA
                     DTRPDLVLVDWMLPDMSGLELTRALKRDRETRELPIIMLTARAEEGDKVAGLEGGADD
                     YVTKPFSPRELLARINAVLRRSTPTGADELIEFEGLVLDQGSHRVMVGPRTVGLGPTE
                     YRMLEFFMTHPERVYTRDQLLDRIWGGNVYVEERTIDVHIRRLRKALEEFGYDRFVQT
                     VRGSGYRFSARTV"
     gene            1404..>2384
                     /locus_tag="VM713_05300"
     CDS             1404..>2384
                     /locus_tag="VM713_05300"
                     /inference="COORDINATES: protein motif:HMM:NF023236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor protein PhoR"
                     /protein_id="HVH83717.1"
                     /translation="MQPATQMWWFTVGRVFATVLVGLAAGWLLGNLWAGLACALALHL
                     GWVLANLFRLEWWLRHRNFADPPDVGGVWGEIIAQVVRLHRRKRFHKQRFVQLMRQLQ
                     RSTAALPNGVVILNAQREIVWFNRRAAQLLELRTTADLGLRIENLLRAPEFLRYLARQ
                     DYSNPVVIRPAMGEDDTYLSLQVAPYGDGQLLLLVSDVSRQMRLEAVRRDFVANASHE
                     LRSPLTVISGYLETLGQDPALDPDLLGPLAEMRRQAERMTSIIRDLIELSRLEETDEI
                     VGGAPIDVAALLSMLRKDVLARPVHPHEVRVKIESAAELVGDEPEIHSAFS"
BASE COUNT          340 a          817 c          822 g          405 t
ORIGIN      
        1 cgagagcatt ctcggcatga tcgatgaggt gctcgccgag gccggcgctt cgctcgcggg
       61 cctcacggcc atcgcctttg gccgcgggcc ggggtcgttc accggtgtgc gcctcgccgc
      121 gagtgtgacc cagggcctcg cccatggcgg tggggtgccc gtcgtgccgg tgtcggatct
      181 cgcggcggtt gcccagcgaa tcctcgccga cgataccggc gcgcagcgcg tactcgtctg
      241 caacgatgcg cgcatgcgcg aggtgtactg gggctgcttt ggtcgctctg ccgatggcct
      301 cgcagctccc gtgggggagg agctcgtgag cgaccccggc ctcgtggcgc tgcccgccga
      361 ctggtcgcgc ggccccggat tgcgcggcgc cggtaccggc ttcgcggcgt acccggagct
      421 tcgagctgcc tggtccagcg tgctggccgc gatcaccgag gtgcgcccgc gggctgccga
      481 gatcgcgctt ctggcggtcg aagaggtccg cgccgggagg ctccgtcgtc ctgaggaggc
      541 tttaccggtc tacctgcgcg ataacgcggc ccgcccagcc gcgagcggtc actgaactga
      601 aacagctctt gcagacttgt cacgaatctg caacacagcg accctgtaat acgcgcgtgc
      661 cagcagggga aacgccgtta acgatgcccg ctaaacaggt tctcatcgtc gaggacgagc
      721 gcccaatccg cgagatgatc gccttcgggt tgaaacgcgc cggcttcgag gttcgcgagg
      781 ccgaggactg ccgcgccgct cgcgccgcgc tcgccgacac acgtccggac ctcgtgctgg
      841 tcgactggat gctgcccgac atgagcgggc tcgagctgac gcgcgcgctc aagcgcgatc
      901 gcgaaacgcg cgagctgccg atcatcatgc tcacggcgcg cgccgaggag ggcgataagg
      961 tcgcgggcct cgaaggcggc gcggacgact atgtcaccaa gcccttctcg ccgcgggagc
     1021 tgctcgcgcg catcaacgcc gtgctgcgcc gctccacgcc gacgggcgcc gacgagctca
     1081 tcgagttcga ggggctggtg ctcgaccagg ggagccaccg cgtcatggtc ggcccgcgta
     1141 ccgtcgggct cggcccgacc gagtaccgca tgctcgagtt cttcatgact cacccggagc
     1201 gggtctatac ccgcgatcag ctgctcgatc ggatctgggg tggcaacgtg tacgtcgagg
     1261 aacgcacgat cgacgtccac attcgccgcc tgcgcaaggc gctcgaggaa ttcgggtacg
     1321 atcgcttcgt gcagaccgtg cgcggctcgg ggtaccgctt ctctgcccgc accgtctgaa
     1381 acgccggcac ggcaggggca gtgatgcagc ccgcgacgca gatgtggtgg ttcaccgtcg
     1441 ggcgcgtgtt cgcgacggta ctggtgggct tggccgccgg ctggctcctc ggcaatctct
     1501 gggcgggact cgcctgcgcg ctcgcgctgc accttggatg ggttctcgcc aacctgtttc
     1561 ggctggaatg gtggctgcgc caccgcaatt ttgccgatcc tccggacgtc ggcggcgtgt
     1621 ggggcgagat catcgcgcag gtcgtgcggc tgcatcgccg caagcgcttc cacaaacagc
     1681 ggttcgtaca gctcatgcgc cagctgcagc gctcgactgc ggcgctgcct aacggcgtgg
     1741 tgatcctcaa cgcgcagcgc gagatcgtgt ggttcaaccg ccgggcggcc cagctgctcg
     1801 agctgcgcac caccgccgac ctggggctgc ggatcgagaa tctgctgcgc gcgccggaat
     1861 ttctgcgcta cctcgcccgt caggactact cgaacccggt cgtgatccgt ccggcgatgg
     1921 gcgaggacga cacgtacctc tcgctccagg tcgcgcctta cggcgacggt cagctgctgc
     1981 tgctggtgag cgatgtctcg cggcagatgc gcctcgaggc cgtgcggcgc gacttcgtgg
     2041 caaatgcctc ccacgagctg cgctcccccc tcaccgtgat ctcgggctac ctcgagacgc
     2101 tcggccagga tccggctctc gatccggacc tcctcgggcc ccttgccgag atgcgccgcc
     2161 aggccgaacg catgaccagc atcattcgcg acctgatcga gctctcgcgc ctggaggaga
     2221 ccgacgagat cgtcggcggg gctcccatcg atgtcgcggc gctcctctcg atgctgcgca
     2281 aggatgtgtt ggcgcggccc gtgcacccgc acgaggtgcg ggtgaagatc gagtccgccg
     2341 cggagctggt cggggacgag cccgagatcc attcggcgtt ctcg
//
LOCUS       DATLKL010000406         5335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_151524, whole genome shotgun sequence.
ACCESSION   DATLKL010000406 DATLKL010000000
VERSION     DATLKL010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151524"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VM713_05305"
     CDS             <1..356
                     /locus_tag="VM713_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HVH83718.1"
                     /translation="RRAPQRPGARLALHKPCTQQHALRCADRTEELLRALGTEPLPVA
                     EAHMCCGSAGTYSLLQGELARSLRARKLESLLAPKPDLILSANIGCITHLAAASEVPV
                     WHWIEWVDRGLNGPA"
     gene            complement(391..972)
                     /locus_tag="VM713_05310"
     CDS             complement(391..972)
                     /locus_tag="VM713_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVH83719.1"
                     /translation="MSRVVSFRAMQDGTREDYLLLDESERAYAAGLADRVLEGLARLD
                     HSLEGYPVSRLGHSLQAATRAARDGADEELVAAALIHDIGDELAPYNHAEFAAAILRP
                     YVRPEVTWIVAQHGLFQNYYYVHHLGGDRNARERFRDHPWFEACAHFCASFDQCSFDP
                     GYRSEALGYFEPLVRRIFARTPHDPRYVSGPVG"
     gene            complement(969..2069)
                     /locus_tag="VM713_05315"
     CDS             complement(969..2069)
                     /locus_tag="VM713_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVH83720.1"
                     /translation="MLERADGRTETLHPLWLRERCRDPVSMDPRTQQRLQDPSDFDLN
                     LRLTAISQPSPGLFQLTFSDGHQATFSAADILEEAALAANSHDIPPAQLWDGSLGELP
                     RARWRAEPAESELAAWLESFLTFGFVIFEGVPTEPGMVLKVGARFGFTRETNFGALFN
                     VRSTPNANDLAYTSIALDPHTDNPYRTPVPGIQLLHCLVNETSGGLSTLVDGFAVAEA
                     LRAREPEAFRILSTTPVRFKYIDTDTELTASAAPIELDVTGELKAIHFSPRLDFVPLF
                     PRAQLEAYYRARRSFDHALRAPDFEIRFLLRDGDLVMFDNCRLLHGRTGFDPAEGLRH
                     LQGCYIDIDGPRSLYRVLRRRRAQGSDVRRSA"
     gene            2271..3377
                     /locus_tag="VM713_05320"
     CDS             2271..3377
                     /locus_tag="VM713_05320"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HVH83721.1"
                     /translation="MTLSRRDLLTASALLLAGPGMALRAAARESGAKGLKNPIILCWN
                     ENPYGPSPAARAALSEAIGASCRYPDDELEPLVQMLATREGVSTDAVVTGTGSGELLR
                     SLGMLYARDGGEIVAAEPTYAELTGYAAGCGAKIRFVPVDGQLRLDLAAMHAALSPRT
                     RAVYLCNPNNPTGTALRAADIRAFVAALPASVTTIVDEAYMDFAVGPDIGSVADVTRG
                     ERRVVVLRTFSKIHGMAGIRCGYAIARPDVAQELAAARMTTPNVFAVRAARASLGDQA
                     FLADCRRRILASRVRITTELARLRLPYAEPQGNFIFFDTGMPLKHFTELMRARNILVG
                     RRFAPYENWCRITIGTEPEVDAFLAALPGVTGRA"
     gene            complement(3391..4149)
                     /locus_tag="VM713_05325"
     CDS             complement(3391..4149)
                     /locus_tag="VM713_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83722.1"
                     /translation="MIAVLREVSPSLAACELSFLERAPIDVERARAQHAHYAAELTAL
                     GCSVEWLAPLPANADAVFVEDTAVVLPEVAVITRPGVASRRNEVESVAASLATHRPLR
                     RIGAPGTLEGGDVVCIGRNLYVGASARSNAAGIEQLRSALAPFGYRVASVAMRDCLHL
                     KSAVTFIPPDVLPVNAAWVEPQVFDARRVVEVDAAEAFAANTLTVQGVTLVSAAFPRT
                     ELRLRGAGVTTRALDVSELQKAEAALTCMSLMLV"
     gene            complement(4220..5164)
                     /locus_tag="VM713_05330"
     CDS             complement(4220..5164)
                     /locus_tag="VM713_05330"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVH83723.1"
                     /translation="MTSARLERWITRKFRLRHVELIAELYDCRSILKASRRLSLTQPT
                     LTKALRDVESTLGVRLFERSNRGLEPTPYGEIFARHAKIVLAQLRHAAEELESLRAGY
                     SGKVTVGTLLAASASILPDAIALLKKQRPGVAISVAVGTYDILMPSLLAGDLDMVLGR
                     LPEEGRSRALVYEEFYAEPICVVTRRGHPLARKRRLTLRDLANEAWLLPLPETALRRQ
                     IERAFVEAGAPLPRNVIESVSILTNRALLRKSDSVGVMPYHVALDDVEQGLLAILPVR
                     LKSIESPVGAILRAPGTLPPAASALLDCLRLAGKEVPK"
BASE COUNT          815 a         1856 c         1874 g          790 t
ORIGIN      
        1 agcgccgcgc gccgcagcga ccgggagcgc gcctcgcgct ccacaagccc tgcacccagc
       61 agcatgcgct gcgctgcgcc gaccgcaccg aggagctgct gcgtgcgctc gggaccgagc
      121 cgctgccggt ggccgaagcg cacatgtgct gcggctcggc gggaacctat tcgctactcc
      181 agggcgagct ggcgcgctcg ctgcgcgcgc gcaagctcga gagcctgctc gcgccaaaac
      241 ccgacctcat cttgtcggcc aacatcggct gcatcaccca cctcgccgcg gcgagtgagg
      301 tgccggtatg gcactggatc gaatgggtgg atcgcgggtt gaacggaccc gcctgagatc
      361 gttgcaggcc tacgcggcgc ggcccgtctc tcagcccacc ggacccgaca cgtagcgcgg
      421 gtcgtggggc gtgcgggcga agatccgccg caccagcggc tcgaagtagc cgagcgcctc
      481 cgagcggtac ccgggatcga aggaacattg gtcgaagctc gcgcagaagt gcgcgcacgc
      541 ctcgaaccac ggatggtcgc ggaagcgctc gcgcgcgttg cgatcgccgc cgaggtgatg
      601 cacgtagtag tagttctgga agaggccgtg ctgcgccacg atccaggtca cttccggccg
      661 cacgtacgga cgcaggatcg ccgcggcgaa ctccgcgtgg ttgtagggcg cgagctcatc
      721 gccgatgtcg tggatcagcg ccgccgccac cagctcctcg tccgccccat cccgcgcggc
      781 gcgcgtcgcg gcctggagcg aatgtccgag ccggctgact ggatagccct cgagggagtg
      841 atcgagccgg gcgaggccct cgagcacgcg gtcggcaagg cccgcggcgt aggcgcgctc
      901 ggactcatcg agcagcaggt aatcttcccg cgtgccgtcc tgcatcgccc ggaagcttac
      961 gacgcggctc acgcgctgcg cctcacgtcg gacccttgag cacgcctgcg tcgcagcacg
     1021 cggtagaggc tccgcggtcc gtcgatgtcg atgtagcagc cctgcaggtg ccgcagccct
     1081 tccgctggat cgaagccggt gcgcccgtgc agcagtcggc agttgtcgaa catcacgaga
     1141 tcgccgtccc tgaggaggaa gcggatctcg aagtccggcg ctcgcagcgc gtggtcgaag
     1201 gaacgtcgcg cccgatagta agcctcgagc tgcgcgcgcg ggaacagcgg cacgaaatcc
     1261 agccgcgggc tgaagtggat ggccttgagc tcgcccgtga cgtcgagctc gatcggcgcg
     1321 gcgctcgcgg tgagctcggt atcggtgtcg atgtacttga atcggaccgg ggtggtggaa
     1381 aggatgcgga aagcctccgg ctcgcgcgcg cgcaacgcct ccgccacggc aaaaccgtcg
     1441 accagcgtcg acaggccccc gctcgtctcg ttcaccagac aatgcagcag ctggatgcca
     1501 ggcaccggcg tccggtaggg attgtcggta tgcgggtcga gcgcaatcga ggtgtaggcc
     1561 agatcgttgg cattcggcgt cgagcgcacg ttgaagagcg ccccgaagtt ggtctcgcgc
     1621 gtgaagccga atcgcgcacc caccttcagg accataccgg gctcggtggg caccccttcg
     1681 aagatgacga aaccgaaggt gagaaaggac tcgagccacg cggccagctc cgactcggca
     1741 ggctcggcgc gccagcgcgc ccgcgggagc tcgccgagcg agccgtccca cagctgcgcc
     1801 ggcggaatgt catggctgtt agcggcgagt gcggcctcct cgaggatatc ggcggcgctg
     1861 aaggtcgcct gatggccgtc gctgaacgtc aactggaaga ggccggggga cggctgggag
     1921 atcgcggtga gcctcaggtt gaggtcgaag tcggacggat cctgtagacg ctgctgcgtc
     1981 cgcggatcca tgctcaccgg gtcgcggcag cgctcgcgca gccacagcgg atgcagcgtc
     2041 tcggtacgcc cgtcggcgcg ctcgagcacg agctcgtgag gcgcgccaag ccgcactcgc
     2101 gcggggcgtt gagtgaccat ggccgcgagt ctagcctcgg ctgttcggca gtgtccacgc
     2161 cgacccccat cgcgacggct ggggcgctgt tgccatgctc ctcttgtcgc cggccgcgcg
     2221 cttcctccag cataggctat cgggccaagg cccggacggg agcactcgcc atgaccctga
     2281 gccgccgcga cctgttgacc gcctccgcgc tgcttttggc tgggcccggc atggcgctgc
     2341 gcgccgcggc gcgcgagtcg ggcgccaagg gcctcaaaaa tcccatcatc ctctgttgga
     2401 acgagaatcc ctacggcccc tcccccgcgg cgcgcgcggc gctgagcgag gcgatcggtg
     2461 cgagctgtcg ctaccctgat gatgagctcg agcccctggt gcagatgctg gcgacccgcg
     2521 agggcgtgag caccgatgcc gtcgtcaccg gaacggggtc aggcgagctg ctgcgctcgc
     2581 tcggcatgct gtacgcgcgc gacggcgggg aaatcgtcgc cgccgagccc acctacgccg
     2641 agctcaccgg ctatgccgca gggtgcggcg cgaagattcg cttcgtcccc gtcgacggcc
     2701 agctgcgcct cgacctcgcg gcgatgcacg cggcgctctc gccgcgcacc cgggccgtgt
     2761 acctctgtaa tcccaacaac ccgaccggca cggcgctccg cgccgccgac atccgtgcct
     2821 tcgtcgcggc actgcccgcc tccgttacga cgatcgtcga tgaggcctac atggactttg
     2881 cggtggggcc ggacatcggc tcggtggcgg atgtcactcg cggcgagcgt cgcgtcgtgg
     2941 tgctgcgcac cttctccaag atccacggca tggcgggcat ccgctgcggc tacgccattg
     3001 cgcgccccga cgtggcgcag gagcttgcgg ccgcgcgcat gacgaccccg aacgtattcg
     3061 cggtgcgcgc cgcgcgcgcc agcctcggcg atcaggcctt cctcgccgat tgccggcggc
     3121 ggatcctcgc gagccgcgta cgcatcacta ccgagctcgc ccgcctcagg ctcccctacg
     3181 ccgagccgca ggggaacttc atcttcttcg acaccggcat gccgctcaag cacttcaccg
     3241 agctcatgcg cgcgcgcaac atcctggtgg gacgccgctt cgccccctat gagaactggt
     3301 gccgcatcac catcgggacc gagcccgagg tcgacgcgtt cctcgcggcg ttgcccgggg
     3361 taaccgggcg cgcttagctg cgcttgcgtc ctagaccagc atcagactca tgcaggtgag
     3421 ggcggcttct gccttctgca gctcgctcac atcgagcgcg cgcgtggtga cccccgcccc
     3481 gcgcagccgc agctcggtgc gtgggaaggc cgcgctgacg agcgtcacgc cctgcacggt
     3541 gagcgtgttg gcggcgaatg cctcagccgc gtccacctcg actacgcggc gcgcgtcgaa
     3601 cacctgcggt tccacccacg ccgcgttcac ggggaggaca tcggggggaa tgaaggtgac
     3661 tgcactcttc aagtgcaggc agtcgcgcat ggcgacgctc gcgacgcggt agccgaacgg
     3721 cgcgagcgcg ctccgcagct gctcgatgcc ggctgcgttg ctgcgcgccg aggcgccgac
     3781 atagaggtta cgcccgatgc acacgacgtc cccgccctcg agagtgccgg gtgcgccgat
     3841 gcgccgcagc ggacgatgcg tcgcgaggct cgccgcgaca ctctcgacct cgttgcgacg
     3901 cgaggcgaca ccgggccggg tgatgacggc gacctcggga agcaccaccg cggtgtcctc
     3961 gacgaacacg gcgtcggcgt ttgccgggag cggtgcgagc cactcgacgc tgcaaccgag
     4021 cgcggtcagc tcggccgcgt agtgcgcgtg ctgcgcgcgc gcccgctcga cgtcgatcgg
     4081 cgcgcgctcg aggaagctca gctcgcaggc cgcgaggctc ggacttactt cgcgaaggac
     4141 ggcgatcatc gcgcagcgag catagcccag gcccgcgagc ggcggaacgc gcgcgccgcg
     4201 cggcggcggc agcgctgcct cacttgggga cctctttacc ggcgagccgc aggcagtcga
     4261 gcagcgcgct cgccgccggc gggagcgtac ccggcgcgcg caggatcgcg cccaccggac
     4321 tctcgatcga cttgagcctc accggcagga tggccagcag cccctgctcg acgtcatcga
     4381 gcgccacgtg gtacggcatc accccgaccg agtcggactt gcgcagcagc gcccggttgg
     4441 tgaggatcga cacggactcg atcacgttgc gcggcagcgg tgcccccgcc tcgacgaagg
     4501 cgcgctcgat ctggcgtctg agtgccgtct cgggcagcgg cagcagccat gcctcgttgg
     4561 cgaggtcgcg taaggtgagt cgccgcttgc gcgcgagcgg atgtccgcgc cgcgtcacga
     4621 cgcagatcgg ctcggcgtag aattcttcgt acaccagcgc acggctgcga ccctcctcgg
     4681 gcagccgccc gagcaccata tcgagatcac cggcgaggag cgagggcatc aggatgtcgt
     4741 aggtgccgac cgcgacgctg atggcaactc cagggcgctg cttcttcaag agcgcgatgg
     4801 cgtcgggaag gatgctggcg gaagcagcca gcagggtgcc caccgtcact ttgccgctgt
     4861 agcccgcgcg caggctctcc agctcctcgg ccgcatgccg cagctgcgcc agcacgatct
     4921 tggcgtggcg cgcgaagatc tcgccgtacg gggtgggctc gagtccgcgg ttgctgcgct
     4981 cgaagagcct gaccccgagg gtcgactcga cgtcgcgcag tgccttagtg agcgtcggct
     5041 gggtgagact caggcgcctg gaggccttga ggatgctgcg gcagtcgtac agctcggcga
     5101 tcagctcgac gtggcgcagg cggaacttgc gcgtaatcca gcgttcgaga cgggccgatg
     5161 tcatggccgc cgatcataac ggttcggccg cgccgcgcgc gccgcccgga gctacacgct
     5221 gagcggcgcg ccggtgcccg cgcgtgtcgg gcgcccggtt tcgatgccga agaaggccgc
     5281 ggcgttcgag tggaggactt tggcgcgggt cgcagcagcg agcgcgggat gcgag
//
LOCUS       DATLKL010000407         1513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_151531, whole genome shotgun sequence.
ACCESSION   DATLKL010000407 DATLKL010000000
VERSION     DATLKL010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151531"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..238)
                     /locus_tag="VM713_05335"
     tRNA            complement(167..238)
                     /locus_tag="VM713_05335"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Thr,seq:tgt)
     gene            complement(295..1305)
                     /locus_tag="VM713_05340"
     CDS             complement(295..1305)
                     /locus_tag="VM713_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83724.1"
                     /translation="MNLIDSVAQCHTPLVVRDHTSGRVTALNNTADCGALVARCPLRY
                     VLSDDLTRLCAELAYSKGARTIACADLLHIPAEALWMEWCNAPWNKALQQYGFPLVEA
                     GAQWVGRRGAWIRATRDGRRGLVRTFWSVPAGEVLASSVEAYFDLDTTTGAEPHPLDG
                     EPGLAGRVIDSDSVDDLLGRCFRFRYEQSWSEYYESAGLAREQSFALWRHAVGTIAMD
                     IPMLLAFFLLLATRSGLPQRVQTLEQLNRRRLRGGKAPLLEHIEVTAPLLPEYRDYQH
                     SEPFASRKSPRLHHVRGHLVRRGSLIFWRVPHLRGSARAGAVGTRTVTWTFARGAAGR
                     IH"
BASE COUNT          271 a          508 c          483 g          251 t
ORIGIN      
        1 agcagccgat agacgtacag cgctttatca cttgcagaat ccgtcgactc gaactcgacc
       61 gccttgcggt gctccgcata ttcaacgcct gcggccaagg ctgctttgaa caattccgct
      121 tcgtgcttga acttcttcac acgctaagcc cgtaggacat tcttgatgcc ggctgagaga
      181 tttgaactcc cgaccaacgg tttacaaaac cgctgctcta ccactgagct aagccggcgt
      241 tgacggtatc ccgcgcgagc ggattgtacc gatggcggtg ccccgccgcc cgcttcagtg
      301 tatgcgcccg gcggcgcccc tggcgaaggt ccaggtaaca gtacgcgtgc cgacggcgcc
      361 ggcgcgggcg ctgccgcgaa gatgcggcac gcgccagaag atcaggctgc cgcggcgcac
      421 gaggtgcccg cggacgtgat gaagccgcgg actcttcctg ctcgcgaatg gctcgctgtg
      481 ctggtagtcg cggtactcgg gcagcagcgg agccgtcacc tcgatgtgtt ccagcagcgg
      541 cgccttgccg ccccgcaagc gcctgcggtt gagctgctcg agcgtctgca cgcgctgggg
      601 caagccgctg cgcgtcgcga gcaggagaaa gaatgcgagc agcatcggaa tgtccatcgc
      661 aatcgtgccc acggcgtgcc gccacaacgc aaagctctgc tccctcgcga gcccggcgct
      721 ctcgtagtac tccgaccagc tctgctcgta gcgaaagcgg aagcagcggc cgagcaggtc
      781 gtcaaccgag tcgctgtcga tgactctccc ggcaagtccc ggctcaccgt cgaggggatg
      841 cggctccgcg cccgtcgtcg tatcgaggtc gaagtacgcc tcgacgctgc tggcgagtac
      901 ctccccggcc gggacgctcc agaaggtacg gaccagaccg cgccggccat cgcgcgtggc
      961 tcgaatccag gcgccgcggc ggcccaccca ttgggcgccg gcctcgacca gcggaaaacc
     1021 atactgttgc agcgccttgt tccagggtgc gttgcaccac tccatccaca aggcttctgc
     1081 gggtatatgc aggagatccg cacaggcgat cgtgcgtgcg cccttggagt aggccagctc
     1141 ggcacacagc cgcgtcaggt catcgctcag cacgtaccgc agaggacagc gcgcgacgag
     1201 cgcgccgcag tcagccgtgt tgttgagagc cgtcacccta ccgctcgtat ggtcgcgcac
     1261 cacgagcggc gtgtgacact gcgcgacgct gtcgatgaga ttcacgccgc caacgtagca
     1321 gcgtgcagcg gggctcaaga ataaatgtga cgaagtcgcg tgctgccgtg acgacgcccg
     1381 cgtctgtgcg ttacggagcc ggtgcctccg ttccgtccga ccagcgtgcg accggcacca
     1441 ggaagatagt ttgaggctcg ggaaccgcgc gttcgtcgag cagcaccgga gaacccggaa
     1501 ggttggctcc cca
//
LOCUS       DATLKL010000408         2248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_185324, whole genome shotgun sequence.
ACCESSION   DATLKL010000408 DATLKL010000000
VERSION     DATLKL010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185324"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VM713_05345"
     CDS             complement(<1..488)
                     /locus_tag="VM713_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83725.1"
                     /translation="MSRPTLRHLANVDPVMGSLIRAAGPYRHARETGSEPFQILAQAI
                     AHQQLNGTAANTILARLVASVGQGAFPTPKQVLAAPVEQLRAAGFSRAKVAALKDLGA
                     KALAGVVPDGAALEQLSDEAIIERLTQVRGVGRWTVEMLLMFHLDRPDVLPVDDFGVR
                     NG"
     gene            complement(505..1320)
                     /gene="mutM"
                     /locus_tag="VM713_05350"
     CDS             complement(505..1320)
                     /gene="mutM"
                     /locus_tag="VM713_05350"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HVH83726.1"
                     /translation="MPELPEVETTRRGLARHVRGRSIRALKVYERRLRWPVSAGLPAA
                     VAGQRIVSTGRRAKYLLLTLESGTLLVHLGMSGNLRVVAADSPRVAHDHFDLELDSGC
                     ALRFNDPRRFGSLLYTRGDPHAHPLLAELAPEPLAREFDAPYLWRITRGRRVAVKQLL
                     MNSRLVVGVGNIYASEALFRARIRPRRQARSLTRSECARLVRAVRAVLRSAIRAGGTT
                     LRDYLRTDGSPGYFRQRLFVYERAGLPCRRCGARVRATTQGQRSTYYCPACQK"
     gene            complement(1342..1497)
                     /gene="rpmG"
                     /locus_tag="VM713_05355"
     CDS             complement(1342..1497)
                     /gene="rpmG"
                     /locus_tag="VM713_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HVH83727.1"
                     /translation="MREKIKLLSTAGTGHFYVTTKNKRLHPNKLEVRKFDPVARKHVA
                     YKESKIK"
     gene            complement(1522..1758)
                     /gene="rpmB"
                     /locus_tag="VM713_05360"
     CDS             complement(1522..1758)
                     /gene="rpmB"
                     /locus_tag="VM713_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HVH83728.1"
                     /translation="MSRVCEITGKKPAVGNRVSHANNKKRRRFLPNLHTQRFWLETEK
                     RWVTLRLTTSGMRTIEKKGVDKVVAELRAQGVKL"
     gene            2008..>2248
                     /locus_tag="VM713_05365"
     CDS             2008..>2248
                     /locus_tag="VM713_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83729.1"
                     /translation="MKRTTQVVCALAALFGVSSLSSTAVFADGKDWSDGPVINVASIR
                     TIDGHFDDYMHWLATTYKKQQEAAKKAGLITNYEVI"
BASE COUNT          379 a          750 c          761 g          358 t
ORIGIN      
        1 aatccgttgc gcactccgaa gtcatcgacc ggcagcacgt cggggcggtc gaggtggaac
       61 atgagcagca tctccaccgt ccaccggccg accccgcgca cctgggtgag ccgctcgatg
      121 atcgcctcat cggatagctg ctcgagcgcc gcaccgtcgg gaacgacccc cgcgagcgcc
      181 ttggccccga gatccttgag tgccgcgacc ttcgcgcgac tgaaacccgc ggcgcgcagc
      241 tgctccacgg gagcggccaa cacctgctta ggcgtcggaa aagcgccctg gccgaccgag
      301 gcgacgaggc gcgcgaggat ggtgttggcg gcggtgccgt tgagctgctg atgcgcgatc
      361 gcctgcgcga gtatctgaaa cggctcggaa cccgtctcgc gcgcgtgccg atagggaccg
      421 gcggcgcgga tgaggctgcc catcaccggg tcgacgttcg ccaggtgccg cagagtgggc
      481 cggctcatgg gcgttcctcg ggcgctactt ctgacatgcc ggacaatagt acgtcgagcg
      541 ctgcccctga gtggtggccc gcaccctcgc tccacagcgc cggcagggca gtccggcgcg
      601 ctcgtatacg aagaggcgct ggcggaagta gcccggcgag ccgtcggtgc gcaggtaatc
      661 gcgcagtgtc gtgccgccgg cgcggatcgc cgaccggagc accgcgcgca ccgcgcgcac
      721 gagccgcgcg cactctgagc gcgtcagcga gcgtgcctgg cgccgcgggc ggatgcgcgc
      781 gcgaaacagc gcctcgctcg cgtagatgtt gccgacgccg accaccaggc ggctgttcat
      841 gagcagctgc ttcaccgcga cgcgccggcc gcgcgtgatg cgccacaggt aaggcgcgtc
      901 gaattcgcgc gccagaggct cgggagcgag ctcggcgagc agcgggtgtg cgtgcggatc
      961 gccgcgcgtg tagaggaggc tgccgaagcg gcgcgggtcg ttgaagcgca gcgcgcaacc
     1021 cgagtcgagc tcaagatcga agtgatcgtg cgcgacgcgc ggactgtccg ccgctacgac
     1081 ccgcagatta cccgacatcc ccagatgcac gagcagcgtc cctgactcga gcgtgaggag
     1141 cagatatttc gcacgccggc cggtcgacac gatccgctgc ccggccaccg ccgcgggaag
     1201 tcccgccgac accggccagc gcagacgccg ctcatacact ttaagcgcgc ggatcgagcg
     1261 cccgcgcacg tgccgcgcca ggccacgccg cgttgtctcg acttcgggga gctccggcac
     1321 gcgccgggac gcgcgcggga attacttgat cttcgactct ttgtaggcga cgtgcttgcg
     1381 cgccaccggg tcgaacttgc ggacttcgag cttgttcggg tggagccgct tgttcttggt
     1441 cgtcacgtag aagtggccgg tgccggccgt ggagagcaac ttgatcttct cgcgcatggg
     1501 tgttctcccg ctgcagtccc gtcagagctt cacgccctgc gcgcgcagct cggcgacgac
     1561 cttgtcgacg cccttcttct cgatggtgcg catcccgctg gtcgtgaggc gcagcgtcac
     1621 ccagcgcttc tcggtctcga gccagaagcg ctgcgtgtgc aggttcggca ggaagcggcg
     1681 acgcttcttg ttgttggcgt gagagacgcg gttaccaacg gccggtttct tgccagtgat
     1741 ctcgcagacg cgggacatag ctgcaacctt gagacaaatc agatacttag gagtcttgcc
     1801 aggcggaact gctcgactcc gggccgggct ttataccagc ccggcggcgg gatctcaagg
     1861 cgccagggcg cataaagagc gcacacagcc gcccggacca gcgtaccgtg tgccgcgccc
     1921 cggtgactgg cgggagcaat ttccgcaggc cgggcgctcg accctaacaa gctactaaac
     1981 aaaccttcgg gttcaggagc atccttcatg aaacgcacga ctcaagtcgt ctgcgccctc
     2041 gcggcgctgt ttggcgtgag ctctctttcg agcactgcgg ttttcgccga cggcaaggac
     2101 tggagcgacg gaccggtcat caatgtcgcc tcgatccgca ccatcgatgg gcacttcgac
     2161 gactacatgc actggctggc gacgacctac aagaaacagc aggaggcggc gaagaaggcg
     2221 ggcctcatca ccaactacga ggtgatcg
//
LOCUS       DATLKL010000409         3441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_128945, whole genome shotgun sequence.
ACCESSION   DATLKL010000409 DATLKL010000000
VERSION     DATLKL010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128945"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VM713_05370"
     CDS             <1..251
                     /locus_tag="VM713_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83730.1"
                     /translation="QSIGRASQAIYGRDAVHMGCGGTIPFMGMLGERFPSTQFFITGV
                     LGPHANAHGPNEFLHIEYAKKLTACVSLVLADHARTVS"
     gene            complement(255..851)
                     /locus_tag="VM713_05375"
     CDS             complement(255..851)
                     /locus_tag="VM713_05375"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HVH83731.1"
                     /translation="MNDLSRLPRVVWAAALGAAALAFVAWWVWSWYRYYRARRALRVA
                     VTGGAADHLANALVPDGMGGGFHVDFLLLTMRGVVVIDLRDVRGNIFGGDQMAEWTVI
                     DGALRFTFTNPQSALYDRIAAVKAVAGDVPVEGRIVFTRRGKFPKGLPKWTLMVDALR
                     AEFPSVEYQSHAEATAPLQAGWQRLKAAVKPSYMADMR"
     gene            complement(848..2164)
                     /gene="thrC"
                     /locus_tag="VM713_05380"
     CDS             complement(848..2164)
                     /gene="thrC"
                     /locus_tag="VM713_05380"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVH83732.1"
                     /translation="MSEPFAFTSSRDPSVRVGFSAALLQGLAPDGGLYVPASWPRLSV
                     EDFAGAATLAEFAQPLLAPFLAGDRLAGELTPLAHAAFDFPAPLVPLHADGRLSVLEL
                     FHGPTAAFKDFGARFLAACFARLREARPLTILVATSGDTGGAVAAAFHRRPGIEVAVL
                     FPRGLVSPTQERQLTCWGENVRSLAVRGTFDDCQRLVKEAFLDAGLRARGAFSSANSI
                     NLGRLLPQAVYYAAASLAVWREHGERASFVIPSGNLGNSLACLWARQAGLPIGAVVLA
                     HNANRPVPDYLASGEWRPRPSVATLASAMDVGNPSNMERLRSLYPQLALLREAVSAVS
                     VSDEEIRNRIRAGYRTFGQIWCPHTATAAEAWERLPPEQRAHGRWVLVSTAHPAKFRE
                     IVEPLIGRSVPVPESLARLFARPVRCAEIDATLEALRTALEPQGAA"
     gene            complement(2184..3125)
                     /locus_tag="VM713_05385"
     CDS             complement(2184..3125)
                     /locus_tag="VM713_05385"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HVH83733.1"
                     /translation="MSAAARQRATAFAPASVGNVAIGFDILGFSVEALGDRVSVARRS
                     APGVAIGAVRGIAGELPREPRDNTAGRALLALQESVQPDFGFILEIDKGIPLGSGLGG
                     SAASAVGAVVAANALLPAPCSLQELLKFAMAGEAVASGARHVDNIAPSLYGGLVLTVG
                     IDEPRVKQIPVPAGIRAIIVRPHMFLATAEARAILKGSVELADFVWQTAHLAGFISGC
                     YTDDLDMIRASFEDVVIEPQRQALIPGFQDVRRAALRAGALGCSISGAGPAMFAWALE
                     GSAAAVLEAMRREFDARGLPSDGWIVDMRRAGARVIA"
     gene            complement(3122..>3441)
                     /gene="metL"
                     /locus_tag="VM713_05390"
                     /gene_synonym="metM"
     CDS             complement(3122..>3441)
                     /gene="metL"
                     /locus_tag="VM713_05390"
                     /gene_synonym="metM"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="multifunctional homodimeric enzyme that catalyzes
                     the phosphorylation of aspartate to form
                     aspartyl-4-phosphate as well as conversion of aspartate
                     semialdehyde to homoserine; functions in a number of amino
                     acid biosynthetic pathways; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HVH83734.1"
                     /translation="EFMQELPRYDSMMRGRLESARARGKVLRYIGRLTAAGEASVGLA
                     ELSTSHAFANIALTDNVVRFATRRYCENPLIVQGPGAGPEVTAGGVFADLLRLAAYLG
                     AHL"
BASE COUNT          549 a         1251 c         1179 g          462 t
ORIGIN      
        1 agcagtcgat cggtcgcgcc agtcaggcga tctacggccg cgacgccgta cacatgggct
       61 gtggcggaac gatcccgttc atgggcatgc tgggcgaacg ctttccgagc acgcagttct
      121 tcatcaccgg agttctcggg ccgcacgcga acgcacacgg gcccaacgag ttcctgcaca
      181 tcgagtacgc gaagaagctc accgcgtgcg tctcgctggt gctcgccgac cacgcgcgca
      241 cggtgtcctg agtactagcg catatccgcc atgtagctcg gcttcaccgc ggccttgagc
      301 cgctgccagc ctgcctgcag cggcgccgta gcctcggcgt gcgactgata ctcgacgctc
      361 ggaaattcgg cgcgtagcgc atcgaccatc agggtccact tcggcaggcc cttggggaac
      421 ttgccacgcc gcgtgaagac gatccggcct tcaaccggca cgtcccccgc caccgccttc
      481 accgccgcga tccggtcgta gagcgcactc tgggggttcg tgaaggtgaa gcgcagcgcg
      541 ccgtcgatga cggtccactc cgccatctga tcgccaccga agatgttgcc gcggacgtcg
      601 cgcaggtcga tcactaccac tccgcgcatg gtgaggagca gaaagtcgac gtggaatcct
      661 ccgcccatcc cgtccggcac cagcgcgttg gcgaggtgat cggcggcccc cccagttacc
      721 gcgacgcgca gcgcccggcg ggcgcggtag tagcggtacc agctccacac ccaccacgcc
      781 acaaacgcca gcgccgccgc gccgagcgcc gccgcccaca cgacacgcgg caacctgctc
      841 aagtcgttca cgcagccccc tgcggctcga gcgccgtacg cagcgcctcg agcgtggcgt
      901 cgatttcagc acaccggacg gggcgcgcaa acagccgcgc gaggctctcc ggcaccggca
      961 cactccgtcc aatgagaggc tcgacgatct cgcggaactt ggccggatgg gcggtcgaca
     1021 ccagcaccca gcgcccgtgc gcccgctgct cggggggcag ccgctcccag gcctcggccg
     1081 cggtcgcggt gtgcgggcac cagatttgcc caaaggtacg gtagccggcg cggatgcggt
     1141 tgcggatctc ctcgtcgctg acgctgacgg cgctcaccgc ctcccgcagt agcgccagct
     1201 gcggatagag cgagcgcagc cgttccatgt tgctcgggtt gccgacgtcc atggccgagg
     1261 ccagcgtcgc gacactcggc cgcggccgcc attcgccgct cgcgagataa tcgggcacgg
     1321 gccgattggc attgtgcgcc agcaccaccg cgccgatcgg cagtcccgcc tgccgcgccc
     1381 acaggcaggc gagcgaattg ccgagattgc cgctcggaat gacgaaggaa gcgcgctcgc
     1441 cgtgctcacg ccagacggcg aggctcgcgg ccgcgtagta gaccgcctgc ggcagcaggc
     1501 gtcccaggtt gatgctgttg gcggaggaga acgcaccgcg tgcgcgcagc ccggcgtcga
     1561 ggaatgcctc ctttacgagg cgctggcagt catcgaacgt gccgcgcacg gcgagcgagc
     1621 gcacattctc gccccagcag gtgagctgtc gctcctgggt cggtgagacg aggcccctgg
     1681 ggaagagcac cgcgacttcg atcccggggc gccggtgaaa cgccgcggcc accgcgccgc
     1741 cggtgtctcc ggaagtggca accaggatgg tgaggggacg cgcttcacgc agccgcgcga
     1801 agcaggccgc gagaaagcgc gccccgaagt ccttgaacgc ggcggtcggg ccatggaaga
     1861 gctcgagcac cgacaaccgg ccgtctgcgt gcagcggcac gagcggcgcc gggaaatcga
     1921 aggccgcgtg cgcgagcggg gtcagttcgc ccgccaggcg atcccctgca aggaatggcg
     1981 cgagcagcgg ctgtgcgaac tcggccaggg tcgccgcgcc ggcgaagtct tcgaccgaga
     2041 gccgcggcca gctcgccggc acgtacaggc cgccgtccgg ggcgagcccc tggaggagcg
     2101 cggcgctgaa tcccacccgg acactcgggt cgcgggagct ggtgaaggcg aacggctcgc
     2161 tcacgaccgc gcggctcgcc cactcatgcg atcacccggg cgccggcgcg tcgcatatcg
     2221 acgatccagc cgtcgctcgg gaggccgcgc gcgtcgaact cgcgccgcat ggcctcgagc
     2281 acggctgcgg cgctcccttc gagggcccag gcgaacatcg ccgggcctgc tcccgagatc
     2341 gagcagccga gtgccccggc gcgcagcgcg gcgcgccgca cgtcctggaa tcccggtatc
     2401 agcgcctggc gctgcggttc tatgaccaca tcctcgaacg aagcgcggat catgtcgaga
     2461 tcgtcggtgt agcacccgga gatgaagccc gcgagatgcg ccgtctgcca cacgaagtcc
     2521 gcgagctcga cgctcccctt gaggatcgcg cgcgcctcgg cggtggcgag gaacatgtgg
     2581 ggacggacga tgatggcgcg gatacccgca ggcacgggga tctgcttcac ccgcggctcg
     2641 tcgatcccga cggtgagaac cagcccccca tagagcgacg gcgcgatatt gtcgacatgc
     2701 cgcgcgccgc tcgccaccgc ttcccccgcc atcgcgaact tgagtagctc ctgcaggctg
     2761 cagggcgccg gcagcagcgc gttcgcggcc acgacggccc ccacggcgga tgcggccgag
     2821 cccccgagcc cggagccgag cgggatgccc ttgtcgatct cgagaatgaa gccgaagtcc
     2881 ggttgcaccg actcctgcag cgccagcagc gcgcgacctg cggtgttgtc gcgtggctcg
     2941 cgcggcagct ctccggcgat gccgcgcacg gcgccgatcg cgacgccggg cgcgctgcgc
     3001 cgcgccacgc tcacgcggtc gccgagcgcc tcgaccgaga agccgagtat gtcgaagccg
     3061 atcgccacgt tgccgaccga ggccggcgca aaggcggtgg cgcgctggcg cgcggccgcg
     3121 ctcacaggtg ggcccccagg tacgccgcga ggcgcagcag gtcggcgaac accccgcccg
     3181 cggtcacctc cggtcccgcc cctggcccct gcacgatgag cgggttctcg cagtagcggc
     3241 gcgtcgcgaa gcgcaccacg ttgtcggtga gcgcgatgtt cgcgaaagca tggctcgtcg
     3301 acagctccgc gagtccgacg ctcgcttcgc cggccgcggt cagtcggccg atatagcgca
     3361 gcaccttgcc gcgcgcccgt gcgctctcga gacggccgcg catcatggag tcgtagcgcg
     3421 gcagctcctg catgaactcc t
//
LOCUS       DATLKL010000410         1847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_117654, whole genome shotgun sequence.
ACCESSION   DATLKL010000410 DATLKL010000000
VERSION     DATLKL010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117654"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..1423
                     /locus_tag="VM713_05395"
     CDS             359..1423
                     /locus_tag="VM713_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HVH83735.1"
                     /translation="MFLIQRKYRPLLGLDITTSSVKLIELAMAGGQYRVEAYAAEPTP
                     QNAINEKAIVDAEAVGEAIRRAVKRAGAKSDEVAIAISGDAAITKVIQMPRSLRTGDL
                     ESQVEMQADQYIPFPMDEVSYDFEVLGVNDKDPESNDVLLVATRTENVEQRQAAVKAA
                     GLAAKIVDVEAFALENACKLMTHQMPDGGIDRTIAVVDFGASSTTFSVLRNLKVVYTR
                     DFAFGGQQLTEEIMRTYGLAMEEAGRAKKEGGLPGNYQSEVLDPFIDDMTQQVSRSLQ
                     FYLASGSGREQPEKILVCGGCANIPGVAEVISSRVGIAAEKGDPLGQMKLSSRAKAQA
                     VQRDATALLTACGLALRSFD"
     gene            1425..>1847
                     /locus_tag="VM713_05400"
     CDS             1425..>1847
                     /locus_tag="VM713_05400"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HVH83736.1"
                     /translation="MPRINLLPWREGQRKERKVAFLVALGVAVLAAMVTTFAFYLMFD
                     SMIAGQQRRNQLLRSQIKLLDKQIEEINSLENEKQRFISRMDVIEKLQRSRPGIVHVF
                     DEIVRTLPEGVYLTAVHQADRRLKFEGIAQSSTRVSSFM"
BASE COUNT          339 a          593 c          596 g          319 t
ORIGIN      
        1 gtgcctatga cagcggggtt gccggttcga acgttaacgc actccgccgc gaggggccac
       61 gcgcttcggc gagtacggtt cgaccgctct cctcgctcag cgccgtcccg cttccgatcc
      121 gtctggcgtc acgtcccctc acaggacgct ttggacaaga cgcagcgcca ttggttgact
      181 gaaggccccg cccgcgcggg gcgtgacgcc tttggtggtt cgccttcgca cgtccgatcg
      241 gcgccgcgga accgtgaggc tcctcacgtt caatttaact ttgcgctgat atatagtgtc
      301 tccggattta tacagggcgg tcactttcag gcgtaatagt ctgaaaagga aataaaaaat
      361 gttccttatt cagcggaagt accgaccgct cctcggtctc gatataacga cctcctcggt
      421 caagctcatc gagcttgcca tggcgggcgg ccagtaccgc gtggaggcct atgcggccga
      481 gcccacgccc cagaatgcca tcaacgagaa ggcgatcgtc gatgccgagg cggtgggcga
      541 ggcgatccgc cgcgcggtca agcgcgccgg cgccaagagc gacgaggtcg cgatcgcgat
      601 ctcgggcgat gccgccatca ccaaggtgat tcagatgccg cgctcgctgc gtacgggcga
      661 tctcgagtcg caggtcgaga tgcaggccga ccagtacatc cccttcccga tggacgaggt
      721 gtcctacgat ttcgaagtgc tcggggtgaa tgacaaggat cccgagtcga acgacgtgct
      781 gctggtcgcg acgcgcaccg agaacgtcga gcagaggcag gccgcggtca aggcggccgg
      841 gctcgcggcg aagatcgtcg acgtcgaggc cttcgcgctc gagaacgcct gcaagctcat
      901 gacgcatcag atgcccgacg gcggcatcga ccgcaccatt gcggtggtgg acttcggtgc
      961 gagcagcacc accttcagcg tgctgcgcaa cctgaaggtg gtgtacacgc gcgacttcgc
     1021 cttcggcggc cagcagctca ccgaggaaat catgcgcacc tacggcctcg ccatggagga
     1081 ggccgggcgc gccaagaagg aaggcgggct tccgggcaac taccagtccg aggtgctcga
     1141 tcccttcatc gacgacatga cgcagcaggt gagccgctcg ctgcagttct acctcgcctc
     1201 cggctccggg cgcgagcagc cggagaagat cctggtgtgc ggcggctgcg ccaacatccc
     1261 gggcgtcgcc gaggtgatct cgagccgtgt cggtattgcc gcggagaagg gcgatccgct
     1321 cggccagatg aagttgtcct cacgcgccaa ggcgcaggcg gtgcaacgcg atgcgacggc
     1381 gctcctgacc gcctgcgggc tcgcactgcg gagtttcgac tgacatgcca cgcataaatc
     1441 tacttccctg gcgtgaaggc cagcgcaagg agcgcaaggt cgccttcctg gtggcgctcg
     1501 gcgtcgcggt gctcgccgcg atggtgacca ccttcgcctt ctatctcatg ttcgactcga
     1561 tgatcgccgg gcagcagcgc cgcaatcagc tgctgcgcag ccagatcaag ctgctcgaca
     1621 aacagatcga ggagatcaac agcctcgaga acgagaagca gcgcttcatc tcgcgcatgg
     1681 acgtcatcga gaagctgcag cgctcgcggc ccgggatcgt gcatgtgttc gacgagatcg
     1741 tgcgcacgct ccccgagggg gtctatctca ccgccgtgca ccaggcggac cggcggctga
     1801 agttcgaggg catcgcgcag tcgagcacgc gcgtctcctc cttcatg
//
LOCUS       DATLKL010000411         1781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_140291, whole genome shotgun sequence.
ACCESSION   DATLKL010000411 DATLKL010000000
VERSION     DATLKL010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140291"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /gene="rpoB"
                     /locus_tag="VM713_05405"
     CDS             complement(<1..1716)
                     /gene="rpoB"
                     /locus_tag="VM713_05405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020852081.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HVH83737.1"
                     /translation="MRKNFGKQASILDTPYLLAIQLDSYRTFLQVDKPEEKRDRIGLH
                     AAFQSVFPISSYSGNASLEYVSYRLGEPVFDVKECQLRGLTYAAPLRVKVRLIVLDKE
                     AAGAKKPVKDVREQEVYLGELPLMTDNGTFVINGTERVIVSQLHRSPGVFFDHDRGKT
                     HSSGKLLFSARIIPYRGSWLDFEFDPKDCVFARIDRRRKLPVTIILRALGMNESEILA
                     TFFETNTFYLSRDEVALQLVPQRLRGETATFEIRIGEQVIVEEGRRITARHVRQLEDA
                     KVTRLRVPREYVYGKILAHDVVNTETGEILAKANEVLTAASVEKLIEANIAQVNTLYV
                     NDLDRGPYISDTLRIDPTKTRLEALVEIYRMMRPGEPPTKDAAENLFANLFFNPERYD
                     LSAVGRMKFNRRVGRKEILGPGILYDFKYASSRSDELSKRLVAQYGGMSDVIDVLKVL
                     IDIRNGNGQVDDIDHLGNRRVRSVGEMAENAFRVGLVRVERAVKERLTIAETEPLMPQ
                     EMINAKPVAAAVKEFFGSSQLSQFMDQNNPLSEVTHKRRVSALGPGGLTRERAGFEVR
                     DVHPTH"
BASE COUNT          290 a          550 c          603 g          338 t
ORIGIN      
        1 gtgggtcggg tgcacgtcgc gcacctcgaa tcccgcgcgc tcgcgcgtga gtccccccgg
       61 cccgagtgcc gagacacgcc gcttgtgcgt gacctcggag agcgggttgt tctggtccat
      121 gaactgcgac agctgcgagg agccgaagaa ctccttgacc gccgcggcca ccggcttggc
      181 gttgatcatc tcctgcggca tcagcggctc ggtctcggcg atcgtgaggc gctccttgac
      241 ggcacgctcg acgcgcacga gtcctacgcg gaaggcgttc tccgccatct cgcccaccga
      301 gcgcacgcgg cggttgccca ggtgatcgat gtcgtcgacc tggccgttgc cgttacggat
      361 gtcgatgagc actttcagca cgtcgatgac gtctgacatc ccaccgtact gggcgacgag
      421 tcgcttggac aactcatcgc tgcggctgct cgcgtacttg aagtcgtaga ggatccccgg
      481 accgaggatt tccttgcgcc cgacgcggcg gttgaacttc atgcggccga cagccgagag
      541 gtcgtagcgc tcggggttga agaacagatt cgcgaacagg ttctccgcgg cgtccttggt
      601 cggcggctcg ccgggacgca tcatgcgata gatctccacc agcgcctcga ggcgcgtctt
      661 ggtgggatcg atgcgcagcg tgtcggagat gtacgggccg cggtcgaggt cgttcacgta
      721 cagcgtgttg acctgtgcga tgttggcctc gatgagcttc tcgaccgacg ccgcggtcag
      781 cacctcgttg gccttggcga ggatctcgcc cgtctcggtg ttgactacgt cgtgggcgag
      841 gatcttgccg tagacgtact cgcgcgggac gcgcagccgt gtgaccttgg cgtcctcgag
      901 ctgccgtacg tggcgggcgg tgatacgccg gccttcctcg acgatcacct gctcgccgat
      961 cctgatctcg aaggtcgcgg tctcgccgcg caggcgctgc ggcacgagct gcagcgcgac
     1021 ttcgtcacgg ctcagataaa aggtgttggt ctcgaagaag gtcgcgagga tctcggactc
     1081 gttcatgccg agggcgcgca ggatgatcgt caccgggagc ttgcgccgcc ggtcgatgcg
     1141 cgcgaacacg cagtccttcg ggtcgaactc gaagtcgagc caggagccgc ggtagggaat
     1201 gatgcgcgcg gagaacaaca gcttccccga cgaatgggtc ttgccccggt cgtgatcgaa
     1261 gaacacgccc ggcgagcggt gcagctgcga gacgatgacg cgctcggtgc cgttgatgac
     1321 gaaggtgccg ttgtcggtca tcaggggcag ctcgccgagg tagacctcct gctcgcgcac
     1381 gtccttcacc ggcttcttgg cgccggcggc ttccttgtcg agcacgatca ggcgcacctt
     1441 gacgcgcagc ggggcggcat aggtgaggcc gcgcagctgg cactccttga cgtcgaacac
     1501 cggctcgccg agccggtagc tcacgtactc gagcgaggcg ttgccgctgt agctcgagat
     1561 cgggaacacg ctctggaatg cggcgtgcag gccgatgcga tcgcgcttct cctcgggctt
     1621 gtcgacctgc aggaaggtgc gatacgagtc gagctgaatg gccagcaggt acggcgtatc
     1681 gaggatgctc gcctgcttgc cgaaattctt gcggatgcgc ttcttctagg tgaaggaata
     1741 agccatctgg aggtcacctc aaaaggtcag tgcaacccaa t
//
LOCUS       DATLKL010000412         2785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_173894, whole genome shotgun sequence.
ACCESSION   DATLKL010000412 DATLKL010000000
VERSION     DATLKL010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173894"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VM713_05410"
     CDS             complement(<1..320)
                     /locus_tag="VM713_05410"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HVH83738.1"
                     /translation="MTHHASGPFEVKVTPQKPDTQIARTANLGRLTIDKRFHGDLEAS
                     AKGEMLATQTEVKGSAGYVALERVTGKLQGRSGSFVLQHSATMKRGEPESTITVVPDS
                     GTGEP"
     gene            complement(346..876)
                     /gene="def"
                     /locus_tag="VM713_05415"
     CDS             complement(346..876)
                     /gene="def"
                     /locus_tag="VM713_05415"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HVH83739.1"
                     /translation="MAVRRVLKMGEPLLRQVAAPVTRFDEELAALVADMDDTMRALSG
                     AGIAAPQIGVSLRVVIFELKDNPRYPQLSPVPYTVLVNPQLTVLDPAEEEGWEGCLSV
                     PGMRGLVPRARRLRYQGFDARGAPIDRSVEGFHARVVQHEVDHLDGILFPQRVRDLRN
                     FGFEDALAGQMTPMPD"
     gene            complement(888..2090)
                     /gene="pqqE"
                     /locus_tag="VM713_05420"
     CDS             complement(888..2090)
                     /gene="pqqE"
                     /locus_tag="VM713_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HVH83740.1"
                     /translation="MSAPQVAGASGAAAGGVGPPLWLLLELTYRCPLHCVFCYNPTDF
                     ARTGPELPTGDWLRVLREARALGAVQLGLSGGEPLAREDLEPIVAEAHALGYYVNLIT
                     SGVGMTEERIGALKRAGLDHIQLSFQDSTRELNDFLSSTRTFELKARVAELIRRYDYP
                     MVLNVVLHRLNIDHVGEILAMAERMGAQYVELANTQYYGWAWLNRAHLLPSRAQLERA
                     EEVTRTVRTRVGSRMQIYFVVPDYFETRPKPCMNGLGSVFLAVTPDGAALPCHAARML
                     PGLEFPNVREHDVRSIWYDSAAFNHFRGEAWMKEPCRSCPERQRDFGGCRCQAYLLTG
                     DAANADPVCELSPHHHLVSAAVAEAERAVAAGTAQRAVAAPMAPLTFRDHRISIAVEP
                     RRGAPPAE"
     gene            complement(2087..2365)
                     /gene="pqqD"
                     /locus_tag="VM713_05425"
     CDS             complement(2087..2365)
                     /gene="pqqD"
                     /locus_tag="VM713_05425"
                     /inference="COORDINATES: protein motif:HMM:NF002535.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HVH83741.1"
                     /translation="MSSVNAAARPAIARGMRLQWEPAQEAHVLLYPEGMVKLNGSAGA
                     ILARCDGARTLAEIVADLERAFGATDLSGDVTAFVELALERRWLELRE"
     gene            complement(2362..>2785)
                     /locus_tag="VM713_05430"
     CDS             complement(2362..>2785)
                     /locus_tag="VM713_05430"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83742.1"
                     /translation="IESWLRLAAAVGLERAEVEGLKLLLPGVRFAVDAYVNFARRAPW
                     LEGVCASLTELFAPEIHRQRLAGWPQHYPWIDPTGLSYFQARVGRAQRDVEFALAVTL
                     ERFATRAAQERALEVLKFKLDILWQMNDALALAYGVTP"
BASE COUNT          429 a          982 c          958 g          416 t
ORIGIN      
        1 ggctcgccgg tgccggagtc ggggacgacg gtgatggtcg actcgggctc accgcgcttc
       61 atcgtggcac tgtgctgcag tacgaagctg ccgctgcgtc cctgaagctt gccggtcacg
      121 cgctcgagcg ccacgtagcc agccgagccc ttcacctcgg tctgtgtggc gagcatctcg
      181 cccttggcgc tcgcctcgag atcgccgtgg aatcgcttgt cgatcgtgag ccgcccgagg
      241 ttcgcggtcc gggcgatctg cgtgtccggc ttctgcggcg tgaccttgac ttcgaagggc
      301 ccgctggcgt ggtgagtcat gcgagtctcc ctggggctca tgtgctcagt ccggcatggg
      361 tgtcatctgc ccggcgagcg cgtcctcgaa cccgaagttg cgcaggtcgc gcacccgctg
      421 cgggaagagt atgccatcca ggtgatcgac ttcgtgctgc acgacgcggg catggaagcc
      481 ctcgaccgag cggtcgatgg gggcgccgcg ggcatcgaac ccctgatagc gcagccgccg
      541 cgcgcgcggc acgaggccgc gcatgccggg gaccgacaga catccttccc agccctcctc
      601 ttcggccgga tcgagcacgg tgagctgcgg attcaccagc accgtgtagg gcacggggct
      661 gagctgcggg tagcgcgggt tgtccttgag ctcgaagatc accacgcgca ggctgacgcc
      721 gatctgcggg gcggcgatcc cggcgccgct gagcgccctc atggtgtcgt ccatgtcggc
      781 aaccagcgcg gcgagctcct cgtcgaaacg ggtcacggga gcggccacct gcctgagcag
      841 cggctcgccc atcttcagca ccctgcgaac ggccatcgcg gtgcgcctca ctccgccggt
      901 ggtgcgccgc ggcgcggctc gaccgcgatc gagatccggt ggtcgcggaa agtgagaggc
      961 gccatgggcg cggcgacggc gcgctgcgcc gtgccggccg cgaccgcccg ctccgcctcg
     1021 gccaccgccg cgctcaccag gtgatggtgc ggcgagagct cgcacaccgg gtcggcattg
     1081 gcggcatcgc ccgtgaggag atacgcctgg cagcggcagc cgccgaagtc gcgctgccgt
     1141 tccgggcagc tcctgcaggg ctccttcatc cacgcttcgc ctcgaaagtg attgaacgcc
     1201 gccgagtcgt accagatgct gcgcacatcg tgctcgcgca cgttcggaaa ctcgagtccc
     1261 gggagcatgc gcgcggcgtg acacgggagt gccgcgccgt ccggcgtaac cgcgaggaac
     1321 acgctcccta agccgttcat gcacggcttg gggcgagtct cgaagtaatc cggcaccacg
     1381 aagtagatct gcatcctcga gccaacccgc gtgcgcacgg tgcgcgtcac ctcctcggcg
     1441 cgctcgagct gtgcgcggct cggcaggagg tgcgcgcggt tgagccaggc ccagccgtag
     1501 tactgcgtat tggcgagctc gacgtactgc gcgcccatgc gctccgccat cgcgagaatc
     1561 tcgccaacgt gatcgatatt gaggcggtgc agcacgacgt tgagcaccat cggataatcg
     1621 taacggcgga tgagctccgc cacccgcgcc ttgagctcga aggtgcgggt gctcgaaagg
     1681 aaatcattga gctcgcgcgt gctgtcctga aaggacagct ggatgtggtc gaggccggcg
     1741 cgcttcagcg cgccgatccg ctcctcggtc atgccgacac ccgaggtgat gagattcacg
     1801 tagtagccga gcgcgtgcgc ctccgcgacg atcggctcga gatcctcgcg cgccagcggc
     1861 tcgccacccg agagcccgag ctgcaccgcg ccgagcgcgc gcgcttcgcg cagcacccgc
     1921 agccaatccc cggtcggcag ctccggcccc gtgcgggcga agtcggtcgg gttgtagcag
     1981 aacacgcagt gcagcgggca gcgataggtg agctccagca acagccacag cggtggcccg
     2041 accccacccg ccgcggcgcc actcgcccca gccacctgcg gcgcgctcac tcgcgcagct
     2101 ccagccagcg ccgctcgagg gcgagctcga cgaacgccgt gacatccccg ctgagatccg
     2161 tggcgccaaa ggcgcgctcg aggtccgcga cgatctcggc cagcgtgcgt gcgccgtcgc
     2221 agcgcgcgag gatcgccccg gcactgccgt tgagcttcac catgccctcc gggtagagca
     2281 gcacgtgcgc ctcctgcgcc ggctcccact gcagccgcat gccgcgcgcg atcgccgggc
     2341 gcgccgcggc gttcacggag ctcacggcgt caccccgtag gcgagcgcca gcgcatcgtt
     2401 catctgccag aggatatcga gcttgaactt gaggacctcg agcgcgcgtt cctgcgcggc
     2461 gcgggtcgcg aagcgctcga gcgtcacggc gagcgcgaat tcgacgtcgc gctgcgcacg
     2521 gccgacccgc gcctggaagt aactcaggcc ggtcgggtcg atccatggat agtgctgtgg
     2581 ccacccggcg agacgctggc gatggatctc cggcgcaaag agctcggtga gtgaggcgca
     2641 cacgccttcc agccagggtg cacggcgcgc gaagttgacg tacgcatcga ccgcgaagcg
     2701 cactcccggg agcagaagct tcaagccctc cacctcggcg cgctcgagac ccaccgccgc
     2761 ggcgaggcgc agccaggact cgatg
//
LOCUS       DATLKL010000413         2355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_207762, whole genome shotgun sequence.
ACCESSION   DATLKL010000413 DATLKL010000000
VERSION     DATLKL010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207762"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VM713_05435"
     CDS             complement(<1..238)
                     /locus_tag="VM713_05435"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HVH83743.1"
                     /translation="MATARAQVAPRPEVPDAIKAPAGEQVVLVAHAAGSQIYTCGKGD
                     DGKPQWTLKAPEAQLRDAKGALIGHHDAGPSWKHI"
     gene            479..673
                     /locus_tag="VM713_05440"
     CDS             479..673
                     /locus_tag="VM713_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83744.1"
                     /translation="MVKRAPNWQEMMRLNLELKLVSATSPTRVRSVPGFFTSGTSEPS
                     WLGRTAQGPVMTAGRKARIH"
     gene            complement(710..>2355)
                     /locus_tag="VM713_05445"
     CDS             complement(710..>2355)
                     /locus_tag="VM713_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVH83745.1"
                     /translation="SFVGVVHVLHYPAGQASNTADVALGSYGSARGSAAFALPEWNGY
                     RHSFAAEGESIGFADDREKVSDGRLLYRGALDLGPGELRLDANVSIVRDVPPSPVIRE
                     GTALTTLTPINANFNPADAALDEDKYQLALAYTRPTAWGTWETLASFSRSHMDDIRAF
                     LHPDLSGAADTQNQDRTILDHYFDTHLASDLEEEVTLLVGADLLYGYGRQVTLNGNSA
                     YTVPLDGSVLPPPTTALPVNEVGFVADKRLFAGQYAQLDFKPDARWDLLLGLRLNETD
                     EDKRSSDLTGQYAAQRASRNVTRPTEAAGVSYRVWSAGHDELVLYADFRNAFKPAALD
                     FGPDYQPAVLLPETAKSYEAGVKGAAADGQLSWQAEAFRLDFTNLVVATESGFLTNAA
                     GERLDGIELEARYAATKDLALAVNYAWHDARFTRYQFFDIGSGNYVNVAGNELPLSPK
                     HLASAGVLYEPPRGFNSTLVLTYVGRRFLDEENIAPVGGYARLDATLGYRLGHYELAL
                     EGTNLGNQRPPVSASEFGSESFYLLNARTLWLRLAYRKR"
BASE COUNT          389 a          767 c          812 g          387 t
ORIGIN      
        1 cgatgtgctt ccacgagggt cccgcgtcgt gatggccgat gagcgccccc ttcgcatcgc
       61 gcagctgcgc ctcgggcgcc ttgagcgtcc attgcggctt gccgtcgtct cccttgccgc
      121 aggtgtagat ctgcgagccc gcggcatgcg cgaccagcac cacctgctcg ccggccggtg
      181 ccttgatcgc gtcgggcacc tcgggccgcg gtgcgacctg tgcacgggcg gtggccatac
      241 tgatgaatag ggcggcgagt aagacggcgc gcccgggcga tgcaacggtc agcatcgagc
      301 catcctacgc cgcggccgca ggaaaagccc cccgcggggc ggttccgcgg ccggccggct
      361 cacccgctgc aaattctttg aagtgcgtct gcatgcaagt cacggacaga tcgctttcgg
      421 tgggagcatc ctttttccat cgaatccacc tcgaccgtcg tgagtacgga gacacaccat
      481 ggtcaagcgc gcacccaact ggcaggagat gatgcggctc aacctcgagc tcaagctcgt
      541 ctccgcgact tctccgaccc gcgttcgtag cgttcctggt ttcttcacca gcgggaccag
      601 cgagcccagc tggctggggc gcaccgcgca gggcccggtg atgacggccg gccgcaaagc
      661 caggattcac tagagctcgg ctgcggcacc cgacgcgacc actcgcccgc tagcgcttgc
      721 ggtacgcgag gcgcagccac agcgtgcgcg cattcagcag gtagaacgat tcgctgccga
      781 attcgctggc gctgaccggc gggcgctggt tgccgagatt ggtgccctcg agcgcaagct
      841 cgtagtgacc gaggcgatag ccgagggtgg catcgagccg ggcgtagccg ccgacgggcg
      901 cgatgttctc ctcatcgaga aagcgccggc ccacgtaggt gaggaccagc gtcgagttga
      961 agccgcgcgg cggctcatag agcaccccgg ccgaggcgag atgcttgggc gacagcggca
     1021 gctcgtttcc cgccacgttc acgtagttgc ccgagccgat atcaaagaac tggtaccggg
     1081 tgaagcgggc gtcgtgccag gcgtaattca ccgccagtgc cagatccttg gtagcggcgt
     1141 aacgtgcctc gagctcgatg ccatcgagcc gctcgccggc ggcgttggtc agaaagcccg
     1201 actcggtcgc cacgacgaga ttggtgaagt ccaggcggaa ggcttccgcc tgccagctga
     1261 gctgcccgtc ggccgcggca cccttgaccc cggcctcgta gctcttggcg gtctcgggca
     1321 gcaacacggc gggctgatag tcgggaccga agtcgagcgc cgcgggcttg aaggcattgc
     1381 ggaagtccgc gtagagcacc agctcatcgt ggccggcgct ccagacacgg tagctgactc
     1441 cggcggcctc ggtgggccgg gtgacgttgc ggctggcgcg ctgcgccgcg tactgcccgg
     1501 tgagatcgct cgagcgcttg tcctcgtcgg tctcgttgag gcgcagcccg agcagcaggt
     1561 cccagcgcgc atcgggcttg aaatcgagct gcgcgtactg tcccgcgaac agccgcttgt
     1621 cggcgacgaa gcccacttcg ttgaccggca gcgcagtagt gggcggcggc agcaccgagc
     1681 catcgagggg caccgtgtac gcgctgttgc cgttcagtgt gacctgccgg ccgtagccat
     1741 agagcaggtc cgcgccaacg agcagcgtca cctcctcctc gaggtcgctg gcgagatgcg
     1801 tatcgaaata atggtcgagg atcgtgcgat cctggttctg ggtgtcggcg gcgccgctca
     1861 ggtccggatg caggaacgca cggatgtcat ccatgtgcga gcgcgagaag gaagccaggg
     1921 tctcccaggt accccaggca gtcggccgcg tgtaggcgag ggcgagctgg tacttgtcct
     1981 catcgagcgc ggcatccgcg gggttgaagt tggcattgat cggagtgagc gtggtgagag
     2041 ccgtgccctc gcggatgacc gggctcggtg gcacgtcgcg cacgatgctc acgttcgcat
     2101 cgaggcgcag ctcacccgga ccgagatcga gcgcgccgcg atagagcagc cgtccgtctg
     2161 agactttctc ccgatcgtcg gcgaagccga tactctcccc ctcggccgca aaggagtggc
     2221 gatatccgtt ccactcgggc agcgcgaagg ctgccgagcc gcgcgcggac ccatagctgc
     2281 cgagcgcgac atcggcggta tttgacgcct gcccagccgg gtagtggaga acatggacga
     2341 ccccgacgaa ggagg
//
LOCUS       DATLKL010000414         2253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_117705, whole genome shotgun sequence.
ACCESSION   DATLKL010000414 DATLKL010000000
VERSION     DATLKL010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117705"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VM713_05450"
     CDS             <1..223
                     /locus_tag="VM713_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83746.1"
                     /translation="ALAFLRARALRFRTFMYFAERLKAVTDCHPNRIDAGICEASHAA
                     TTGSVIWVTRREVASKRSRKGAGVTKTQV"
     gene            310..918
                     /locus_tag="VM713_05455"
     CDS             310..918
                     /locus_tag="VM713_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HVH83747.1"
                     /translation="MKTGSAWQRGIAGALSMLLVVGALPRATAGGGAQPMTAILITAR
                     AEVRDPYFGGSVVLVLNNLGPTPVGVVINRPTPLPVARLFPDLKPLAALSDRVYFGGP
                     VAMGSVWFLFRAARPPEHAVRACEGVYLSASRELLLQLLARDKPMNGLRIFMGHSGWG
                     PGQLESEIARGDWTLGHAEPDAIFSGKAERPWPAPPDPKDGT"
     gene            complement(930..2162)
                     /locus_tag="VM713_05460"
     CDS             complement(930..2162)
                     /locus_tag="VM713_05460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH83748.1"
                     /translation="MNRTIMIVALAATYFVFAMLLNSVAPVILQSMLTFGVDKIATST
                     LDACKDLPIALTSLAAAAYIPRLGYRRAMMFGLGFVALACTAMPLVPGFVTTRLMFVA
                     VGVGFALVKVSVYSSIGLLAPAPKQHASLTSLLEGLFMLGVLSTSWVFSLFINRDAPG
                     DPVWLHVYWLLAAVAAAAALLVGFARLDESAVHAAAAAEESPIPGMLNLLRHREVLVF
                     LAAAFLYVLIEQQVQTWLPTFNREVLLLPVTLSVQLASVYSGALAFGRLGASVALRHI
                     YWRNLLIGCLIAAACTLLLSLRASGAGATDWASLPLAAYLPAVLGLFLAPIYPTICST
                     MLSSLPLEKQAPMTGLIVVFSALGGTSGSFITGRIFAATDGRTAFSLGLVPMALLVGA
                     LLLFDRTLRSRPAVAQPA"
BASE COUNT          408 a          741 c          780 g          324 t
ORIGIN      
        1 ggccttggcc ttcctccgcg cgcgcgctct tcgctttcgt acgttcatgt acttcgccga
       61 gcgattaaaa gctgtaaccg attgtcatcc taacagaatc gacgcgggta tttgtgaggc
      121 cagtcacgcc gcaaccaccg gctcggtgat atgggtcaca cggcgggagg tcgcgagcaa
      181 gcgctcgcgt aaaggcgcgg gtgtcactaa aactcaagtg tgaggcccgt cgctgatctc
      241 gggctccacg agcctgctca ggtttcgcag tgactcctgc cagcagcgtg acgggtaaaa
      301 tcgcccctgg tgaaaacggg ctcagcttgg cagcgcggca tcgccggagc gctctcgatg
      361 ctcctggtgg tcggcgcgct gccgcgcgcg acggccggcg ggggcgcgca gcccatgacc
      421 gccatcctca tcacggcgcg ggccgaggtg cgcgatccct acttcggcgg ctcggtggtg
      481 ctggtgttga acaatctcgg ccccaccccc gtgggagtcg tcatcaacag gccgacgccg
      541 cttcccgttg cgcggctgtt cccggatctc aagcccctcg cggcgctctc cgaccgcgtg
      601 tacttcgggg gccccgtcgc aatggggtcg gtgtggttcc tgttccgcgc ggcccggccg
      661 cccgagcatg ccgttcgggc ctgtgaaggt gtctacctga gcgcgagccg ggaactgctg
      721 ctgcagctgc tcgcccgtga taagccgatg aacgggctgc gcatcttcat gggtcactcg
      781 ggctggggac cgggccagct cgagtccgag atcgcacgcg gcgattggac gctcggacac
      841 gccgagccgg acgcgatctt cagcggcaaa gcggagcgcc cctggccggc gccgccggat
      901 cccaaagacg gcacgtaaat cgacggcgtc taggccggct gcgcgaccgc ggggcgcgat
      961 cgcaacgtcc gatcgaacag cagcagggcg cccaccagca gagccatcgg caccagaccg
     1021 agggagaagg ccgtgcggcc gtcggtggcg gcgaatatgc ggccggtaat gaatgacccg
     1081 gaggtcccgc cgagcgccga gaacacgacg atcagtccgg tcatcggcgc ctgcttctcg
     1141 agcgggagcg agctcagcat ggtcgagcag atcgtcggat agatcggcgc gaggaacaga
     1201 ccgagtacgg cgggcaggta ggccgcgagc ggcaggctcg cccagtcggt tgcaccggcg
     1261 ccgctcgcgc gcagcgagag cagcagcgtg cacgccgcgg cgatcaggca gccgatcagg
     1321 agattccgcc agtagatgtg gcgcagcgcg acgctggcgc ccaagcggcc gaacgccagc
     1381 gcccctgagt acacgctcgc cagctgcaca ctgagcgtga ccggcagtag cagcacctcg
     1441 cggttgaagg tcggcagcca ggtctgcacc tgctgctcga tcagcacgta gaggaaggcc
     1501 gcggcgagga ataccagcac ctcgcggtgg cgcagcaggt tcagcatgcc ggggatcggt
     1561 gattcttccg cggcggccgc cgcatggacc gccgactcat cgagccgtgc gaatccgacc
     1621 agcagcgcgg ccgccgccgc gacggcggcc agcagccaat acacgtgcag ccagaccgga
     1681 tcgccgggtg catcgcggtt gatgaacagg ctgaagaccc atgacgtcga cagcacaccc
     1741 agcatgaaca ggccctcgag cagactcgtg aggctggcgt gctgcttcgg tgccggcgct
     1801 agcagcccga tcgaagagta gaccgagacc ttgactaggg cgaagcccac gccgaccgcg
     1861 acgaacatga gtctggtggt gacgaatccc ggcaccagcg gcatcgccgt gcaggcgagc
     1921 gccacgaagc cgagcccgaa catcatcgcg cggcggtagc cgagccgcgg aatgtaggcg
     1981 gccgccgcga gcgaggtgag cgcgatgggc agatccttgc aggcgtcgag cgtcgaggtc
     2041 gcaatcttgt cgacgccgaa ggtcagcatc gactgcagga tgaccggcgc cacgctgttc
     2101 agcagcatcg cgaacacgaa gtacgtggcc gcgagcgcga cgatcatgat tgttctgttc
     2161 atggcgccac cgggagccta acccaacggc tgggaggaga accagtgatg cgcacgcacg
     2221 ccgacgcggc cgcacgtggc agcggcaaat ccg
//
LOCUS       DATLKL010000415         1503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_196591, whole genome shotgun sequence.
ACCESSION   DATLKL010000415 DATLKL010000000
VERSION     DATLKL010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196591"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VM713_05465"
     CDS             complement(<1..501)
                     /locus_tag="VM713_05465"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HVH83749.1"
                     /translation="MTIAARKIRLIMELRRLGITDTRVLSAIERVPRELFVPEPFLDQ
                     AYENVALPIGHGQALSQPAVVARMTEALGPGERMKLLEIGTGSGYQTAILAQLCRRVY
                     TIERRRRLLAEAEQRFAALHLRNITANLGDGNRGWPAQAPFDRILVTAAAAALPELLL
                     QQLKLGG"
     gene            complement(498..1280)
                     /gene="surE"
                     /locus_tag="VM713_05470"
     CDS             complement(498..1280)
                     /gene="surE"
                     /locus_tag="VM713_05470"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HVH83750.1"
                     /translation="MNDAPLDLACARILISNDDGVEATGLKVLERIAHVLSTDVWVVA
                     PETEQSAASHSLTLRVPLRIRELSTRRFAINGTPTDSVLLAVQHILKDRRPDLVLSGV
                     NRGGNLGEDVTYSGTIAAAMEGTLLGVPSIALSQYVIHGQRTRWATVEAHAAAVIRKL
                     VSIRWPKGVLVNVNFPDVPAEQVTGIEVTSQGRRKLGGELERRLDPRGVPYYWIGTMR
                     TEEPSRKGSDLAAIKRGAISVTPLSLDLTHKPTVAGLRKAFA"
     gene            complement(1273..>1503)
                     /locus_tag="VM713_05475"
     CDS             complement(1273..>1503)
                     /locus_tag="VM713_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HVH83751.1"
                     /translation="SCSNCGDFQARRMKARFRRAGEKGTQFVHTLNGSGIAVGRALIA
                     IMENYQQADGSILVPEAIRPYMGGRTRIAADE"
BASE COUNT          229 a          498 c          532 g          244 t
ORIGIN      
        1 accgccaagc ttgagctgct gcagcaagag ttcgggcaac gccgccgccg ccgctgtgac
       61 aagaatgcga tcaaacggcg cttgcgccgg ccagcctcgg ttgccgtcgc ccaagttggc
      121 agtgatgttt cgcaggtgca gcgcggcgaa gcgctgctcc gcctcggcga gcagtcggcg
      181 ccgacgctcg atggtgtaca cgcgtcggca gagctgggcg aggatcgccg tctggtaccc
      241 ggaaccggtg ccaatctcga ggagtttcat ccgctcccct ggcccgaggg cctcggtcat
      301 gcgcgcgacc accgccggct ggctcagggc ctggccgtgg ccgatgggca gcgccacgtt
      361 ctcgtacgcc tggtccagga aaggctcggg gacgaacagc tcgcgcggca cccgctcgat
      421 ggccgagagc acgcgggtat ccgtgatgcc caagcggcgc agctccatga tcaggcggat
      481 cttgcgcgcc gcgatcgtca tgcgaaggcc ttcctgagcc ccgccacggt cggcttgtgc
      541 gtgaggtcga gggagagcgg tgtcacggag atggcgccgc gcttgatggc ggcgagatcg
      601 ctgcctttgc gcgagggctc ctcggtgcgc atggtgccga tccagtaata gggcacgccg
      661 cgcgggtcga gccgtcgctc gagctcgccg ccgagcttgc gccggccctg gctcgtcacc
      721 tcgatccccg tgacctgttc ggcgggcacg tcgggaaagt tcacgttgac gagtacgccc
      781 ttgggccagc gaatgctcac gagcttgcgg atcaccgcag cggcgtgcgc ctcgaccgtc
      841 gcccagcgcg tgcgctggcc gtgaatcacg tactggctga gcgcaatgga gggcacgccg
      901 agcagcgtcc cctccatcgc cgccgcgatg gtgcccgaat aggtcacgtc ctcgccgagg
      961 ttgccgccgc ggttgacccc cgacagcacg aggtcggggc gcctatcctt cagaatgtgc
     1021 tgaacggcga gcagcacgct gtcggtcggc gtgccgttga tggcaaagcg gcgcgtgctg
     1081 agctcgcgga tgcgcaacgg cacgcggagg gtgagcgagt ggctcgcggc gctctgctcg
     1141 gtctcgggcg ccaccaccca cacgtcggtg gagagcacgt gggcgatgcg ctcgagcacc
     1201 ttgagcccgg tcgcctcgac gccgtcgtcg ttcgaaatga gtatgcgggc gcacgcaagg
     1261 tcgagcggag cgtcattcat cggcggcgat gcgcgtgcgc ccgcccatgt agggccgaat
     1321 agcctcgggg acaaggatcg acccgtcggc ctgctgatag ttctccatga tcgcgatgag
     1381 cgcgcggccg acggcgattc cagagccgtt caaggtatgg acgaactgcg ttcccttctc
     1441 gcctgcgcgg cggaaacgcg ccttcatgcg ccgcgcctgg aagtcgccgc agttggagca
     1501 gct
//
LOCUS       DATLKL010000416         2444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_129054, whole genome shotgun sequence.
ACCESSION   DATLKL010000416 DATLKL010000000
VERSION     DATLKL010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129054"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VM713_05480"
     CDS             <1..208
                     /locus_tag="VM713_05480"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HVH83752.1"
                     /translation="PFDGRYVIRGGAVQALEGEPPRGYLVVIAFDSVARARAWYDSPA
                     YQAIQPIRANSTRSRILIVQGVAP"
     gene            368..1006
                     /locus_tag="VM713_05485"
     CDS             368..1006
                     /locus_tag="VM713_05485"
                     /inference="COORDINATES: protein motif:HMM:NF037679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVH83753.1"
                     /translation="MAVIALYMYFRQPIPLRPARDRLLDAAERVVVESGATHLTLDAV
                     AKSAGVSKGGLLYHFPSKEALLDGMLARNFEDVDAEVAKRLESRAGKTAGRGRKGANG
                     ARKPARADVFSERVRVLLEWHAERPAFGAAMVAASAENPEHMAACRAEYRKLLDEFAK
                     LPGGFERTALVLLAVQGLLLGELLHLSPYTPEERSRLVKALQRAADQCRSTP"
     gene            1003..>2444
                     /locus_tag="VM713_05490"
     CDS             1003..>2444
                     /locus_tag="VM713_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVH83754.1"
                     /translation="MTRSARGFPLALLVAAAVSACAVGPDFRTPPPPATTHYTRAPEP
                     VGTVAAPGASGASGIAQTFSTERDIPADWWTLFHSSALDELVRRALTDSPTVAAARAT
                     LLTAAETYKAQRGGLLLPAFDATGSAERQREPGAAFGQPGSAFLFNLFDASVGVTYRL
                     DLFGASRRQVEALRAQSDYQRWQLEAASLALSANVVTTAVNIASLHAQIAALADIIAS
                     ERDQLKVVQRQFDAGAASRSDLLAQQTQVADNEAQLPALEKQLAQAQHRLAVLAGRTP
                     DDTSVPDFTIDQLTLPGELPLSVPAKLVRQRPDVQAAEALLHVSTAQLGVATANLYPQ
                     LNLTGSIGSETVTASDLFKSGTAAWNVGGSLTQPLFHGGELLGLKRAARADLDRATAE
                     YRQAVLAALQDVADTLRALEADARTLQADTITESDAQDTLNITKLQYKVGGVSYLQLL
                     NAERVFLQARQNRVQAEAARYADTAALFQAL"
BASE COUNT          375 a          877 c          820 g          372 t
ORIGIN      
        1 cccgttcgat ggccgatatg tgatccgtgg tggcgcagtg caggctctcg agggcgagcc
       61 tcccagaggc tatctcgtcg tgatcgcctt cgacagcgtg gcgcgggcac gtgcctggta
      121 tgactcgccg gcctatcagg cgatccagcc gatccgggcc aactccacca ggagccgcat
      181 cctgatcgtc cagggcgtcg ctccctgaga cctgactcgc cccgcctcac acccgctaac
      241 gctgtcgtca catcgctgtc ataggtcggg ttcgacccta tcccgatcga gagatggtct
      301 gtggaccgtc tggacggtag ggttgccgcc ttgtcgcgag cgtcagggat cgggccgtat
      361 agagcctgtg gctgtaatcg cactgtatat gtacttccgc caacccattc ccctgcgtcc
      421 cgcacgcgat cgtctcctcg atgcggcaga gcgagtggtg gtcgagtcgg gcgcaacaca
      481 cctgaccctc gatgccgtgg caaagtctgc cggcgtcagc aagggtggac tcttgtatca
      541 ctttccctcc aaggaagcgc tgctcgatgg catgctagcg cggaatttcg aggacgtaga
      601 tgccgaggtc gcaaagcggc tcgagagccg cgccggcaag acggcgggcc gcggccgcaa
      661 gggcgcgaac ggcgcccgca agcccgcgcg cgccgatgtg ttcagcgagc gggtgcgcgt
      721 gctgctggag tggcatgcag aacgtccggc gttcggcgcc gccatggtgg ctgcttccgc
      781 cgagaacccg gagcacatgg cggcctgtcg cgccgagtac cgcaagctcc tcgatgaatt
      841 tgcgaagctc ccgggcggtt tcgagcgcac cgcgctcgtg ctgctcgccg tgcaggggtt
      901 gctgctcggc gagctgttgc acctctcgcc ctacacgccc gaagagcgtt cgcggctcgt
      961 caaggcgctg cagcgcgccg ccgaccagtg caggagcacg ccatgacacg gagcgcacgt
     1021 ggatttcccc tcgccctgct ggtcgccgcg gcggtgtcgg cctgcgcggt cgggcccgac
     1081 ttccgcacgc cgcccccacc tgccacgaca cactacacgc gtgcgcccga gcccgtgggc
     1141 acggtcgcgg cacccggcgc gagcggtgcg agcggcatcg cccagacttt cagcaccgag
     1201 cgtgacattc cggcggactg gtggacgctg tttcactcga gcgcactcga tgagttggtg
     1261 cggcgggcgc tcaccgacag tccgacggtg gcggccgcac gcgcgacgct gctgacggcc
     1321 gcggaaacct acaaggcgca gcgcggcggg ctgctgctcc cggcgtttga tgccacgggc
     1381 agcgccgagc ggcagcgcga gcccggcgcg gccttcgggc agccgggcag cgccttcctc
     1441 ttcaacctgt tcgacgcctc ggtgggcgtg acctaccggc tcgatctgtt cggcgcctcg
     1501 cgccgccagg tggaggcgct gcgcgcgcag agcgattacc agcgctggca gctcgaggcc
     1561 gcgagcctcg cgctgagcgc gaacgtcgtc accacggccg tcaacatcgc ctcgctccac
     1621 gcgcagatcg ccgccctcgc cgacatcatc gcgagcgagc gcgaccagct caaggtcgtg
     1681 cagcgccagt tcgacgccgg cgctgcctcg cgctccgacc tgctcgcaca gcagacccag
     1741 gtggccgaca acgaggcaca gctcccggcg ctcgagaagc agctcgcgca ggcgcagcac
     1801 cgcctcgcgg tgctggccgg ccgcacgccc gatgacacca gcgtgcccga cttcacgatc
     1861 gatcagctca cgctgccggg cgagctgccg ctttccgtcc cggcgaagct ggtgcgccag
     1921 cgcccggacg tgcaggccgc cgaggcgctg ctgcacgtgt cgaccgcgca gctcggagtc
     1981 gcgaccgcca atctctatcc gcagctcaac ctcaccggct cgatcggctc ggagacggtc
     2041 acggcgagcg acctgttcaa gagcggcacc gccgcctgga atgtcggcgg ctcgctgacg
     2101 cagccgctgt tccacggcgg cgagctcctg ggtttgaagc gcgcggcgcg cgccgacctc
     2161 gaccgcgcca ccgccgagta ccgccaggcg gtgctcgccg cgctgcagga cgtcgccgac
     2221 acgctgcggg cgctcgaggc cgacgcgcgc acgctgcagg cggacacgat caccgagagc
     2281 gacgcgcagg acactctcaa catcaccaag cttcaataca aagtgggtgg cgtcagctac
     2341 ctgcagctgc tcaatgccga gcgtgtgttc ctgcaggcgc ggcagaaccg ggtacaggcg
     2401 gaagcggcac gctacgcgga taccgccgcg cttttccagg cgct
//
LOCUS       DATLKL010000417         2169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_106332, whole genome shotgun sequence.
ACCESSION   DATLKL010000417 DATLKL010000000
VERSION     DATLKL010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106332"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..611
                     /locus_tag="VM713_05495"
     CDS             111..611
                     /locus_tag="VM713_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HVH83755.1"
                     /translation="MSRPRLVNIAGFLLCAGLMAYALYAQYGLGLEPCPLCIFQRIGV
                     ILMGVTFLAAGLQYPRGRGRYVYGVALAVFALLTAAVAARQVYIQSLPPGTLPSCGAP
                     LAMMLRFMPVTEVVRKVLSGSGECGEVNWTFLGLAMPAWVLIWSVLLGGVGALANFRR
                     EASAAR"
     gene            complement(583..1710)
                     /gene="dapE"
                     /locus_tag="VM713_05500"
     CDS             complement(583..1710)
                     /gene="dapE"
                     /locus_tag="VM713_05500"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HVH83756.1"
                     /translation="MSQVLKLAENLIARRSVTPADEGCQQLIGERLAALGFRVEPLRF
                     GNVENLWARRGVAPPVFCFAGHTDVVPTGPLEEWRSDPFVPTLRDGLLYGRGAADMKT
                     GLAAMVTATEEFVRAHPTHRGSIAFLITSDEEGPSVDGTKRVAETLAGRGERIDWCLV
                     GEPSSETTIGDTIKVGRRGSLSGRLTVHGVQGHVAYPQLAENPVHTLAPALAELTRRV
                     WDEGTEHFQPTTFQVSNLNAGTGAPNVIPGELRARFNLRYSPVQTLERLKGTVEEILR
                     RHGVRYTIEWYVSGEPFYTPPGVLSDAVGAAVQQVTGARPRLSTGGGTSDGRFIAPLG
                     AQVVELGVVNASIHKVNECVRAADLDALESMYVNVLRKLLA"
     gene            1971..>2169
                     /locus_tag="VM713_05505"
     CDS             1971..>2169
                     /locus_tag="VM713_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVH83757.1"
                     /translation="MNLRRFQRVLTVLLTAAACAGAVQAADISGAGATFPYPIYSKWA
                     DAYKQQTGIGLNYQSIGSGSGI"
BASE COUNT          339 a          731 c          722 g          377 t
ORIGIN      
        1 gcgtaaccca tgcgaatcag ccgtgcgcat gacccatgcg catgacctat gcgcatgacc
       61 cgcgtaccac accgccctac ggggcaccgc tgatccgtta aactgaccga atgtcgcggc
      121 cccgcctggt gaacatcgca gggttcctct tgtgcgcggg cctcatggcc tacgcgctct
      181 acgcccaata cggcttgggg ctcgagccct gcccgctgtg catcttccag cgcatcggag
      241 tcatcctcat gggtgttacc ttccttgccg ccgggctgca gtacccgcgc ggacgcggac
      301 gctatgtcta tggggtggcg ctggcggtgt ttgcgctgct cacggcggcc gtcgcggcac
      361 gccaggtcta catccagagc ctgccgccgg gcacgctgcc ttcctgcggc gccccgcttg
      421 ccatgatgct gcgcttcatg cccgtcaccg aagtggtccg caaggtgctg agcggaagcg
      481 gggagtgcgg tgaggtgaac tggacctttc tcggcctcgc catgcccgcc tgggtgctga
      541 tctggtcggt gttgctcggc ggcgtcggcg cgctcgccaa cttcaggcga gaagcttccg
      601 cagcacgttg acgtacatgc tctcgagggc gtcgagatcg gcggcccgca cgcactcgtt
      661 gaccttgtgg atcgaggcgt tcaccacgcc gagctccacc acctgagctc cgagcggcgc
      721 gatgaagcgc ccgtcggaag taccgccgcc ggtcgagagc cggggtctcg ctcctgtcac
      781 ctgctgcacg gccgcgccga ctgcgtccga cagcacaccg ggcggggtgt aaaagggctc
      841 gcccgagacg taccactcga tcgtgtagcg cacgccgtgc ctgcgcagga tctcctccac
      901 cgtgcccttc agtctctcga gtgtttgcac cggcgagtag cgcaggttga agcgggccct
      961 gagctccccg ggaatcacgt tcggggcgcc cgtgccggcg ttgaggttcg agacctggaa
     1021 ggtggtcggc tggaaatgct cggttccctc atcccacacg cgccgggtga gctccgcgag
     1081 cgccggcgcc agggtgtgca ccggattttc ggccagctgg gggtaggcga cgtgaccctg
     1141 cacgccatgc accgtgagcc gaccgctgag cgagccgcgc cgcccaactt tgatggtgtc
     1201 tccgatggtg gtctcgctcg aaggctcgcc gaccaggcac caatcgatgc gctcaccgcg
     1261 cccggcaaga gtctccgcta cgcgcttggt gccgtcgacc gacgggcctt cctcgtcgct
     1321 cgtaatgaga aaggcgatcg agccgcggtg cgtgggatgg gcacggacga actcctcggt
     1381 ggcggtgacc atggccgcga ggccggtctt catgtctgcg gcgccacgcc cgtagagcag
     1441 gccgtcccga agggtcggca cgaacggatc gctgcgccac tcctcgagcg ggcccgtggg
     1501 gacgacgtcg gtgtgcccgg cgaagcagaa aaccggcggc gccactcccc ggcgtgccca
     1561 gaggttctcc acgttgccga agcgcagcgg ctcgaccctg aagccgagcg cggcgagccg
     1621 ctcgccgatc aactgctgac agccctcatc cgcgggggtc acggagcgcc gggcgatcag
     1681 gttttcggca agcttcagga cctgggacat ctgggctggc ggctccttgg ctcgccgcag
     1741 gcgttcgcga ccaagcggct cgcgtttata cggtgaggcg gccggccggg caaggcgtgt
     1801 gctgtcacga cggtgaaaac tctcccagca agacttagcc cgagcggcgg cgggcatgcc
     1861 gccgggtcct gtcatcgaag cgtcacatta ttgtcatcta atgcgcgcga gagggctaag
     1921 accctcgaga ccgggacacc cgagcgttcc ccgactgcag gagttgagcc atgaatttac
     1981 gaagatttca gcgggtcctg accgtcctac tcactgcggc tgcgtgcgcg ggagcggtgc
     2041 aggcggccga catttccggc gccggggcaa ccttccctta tccgatctat tcgaagtggg
     2101 ccgacgccta taagcagcag accggaatcg gtctcaacta ccagtcgatc ggttccggca
     2161 gcggcatca
//
LOCUS       DATLKL010000418         4094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_15972, whole genome shotgun sequence.
ACCESSION   DATLKL010000418 DATLKL010000000
VERSION     DATLKL010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15972"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..608
                     /locus_tag="VM713_05510"
     CDS             75..608
                     /locus_tag="VM713_05510"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HVH83758.1"
                     /translation="MSQVKHRCALRPLGAALACLGYLLIAGCLSADDDLNRFIEETKH
                     EPGGRVEPLPEIKPYETYVYADSEMRSPFMPSAPGAGAGLAGVRPDSKRNREFLEQFS
                     LDTLRMVGTLRLGGSMFGLVQTKDGLVHRVAVGEHLGQAEGKITEISPAKISLVEIVP
                     DSLGGYMERPAALALNE"
     gene            632..2830
                     /locus_tag="VM713_05515"
     CDS             632..2830
                     /locus_tag="VM713_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HVH83759.1"
                     /translation="MHASSLLKRILGTAAWVVIAGAFAVAPVPPALAADQPSLQSVDV
                     QPLAGQQLQLTLRLSGAAPEPLSFTIDNPARISLDLPGTTLSLPSRRIDVHAAGLDTI
                     IAAETKDRTRVVLNLDKLVPYITRVEGNDIIVLLGSGAAGTVATTRGPSVVSGAGAVG
                     SGTVAASSSGVRELRSIDFRRGTDGAGRVVVRLSDPHIRISLKQVGSQIVVDFTDAGV
                     PANLLRRFDATDYGTPVTGFDVSRVGNGARIAINATGDYEQLAYQSDNQYVVEVAPRR
                     KANATAEEKPVYTGERLTLNFQDIDTRAVLQLLADASGQNIVVSDSVSGNVTLRLQNV
                     PWDQALDIVLKTKGLDKRRDGNVIIVAPQAELATREKAELAARKDVSELAPLRSEYLQ
                     VNYAKAQDLAALIKSQNNSLLSPRGSVAVDERTNTLLMLDTADKLADIRRLVATLDIP
                     VRQVLIESRIVIVNNDFERQIGAIFGITAFHQNGSTLYNTTGTAAGIDTQATTFITGQ
                     NAINNAINAGTTPVPLSTYGIPAATNRYNINLPVTSPAGSIALGLVTNNVLVDLELNA
                     AQAETQANIISSPRVITANQKEATIEQGIEIPYQQSASSGATTIQFKKAVLQLKVTPQ
                     ITPDNRIILDLDVRDDSVGTIVVASGGVNVPTINTREISTQVLVNDGQTVVLGGILQT
                     TQREDDTKVPFLGDIPILGHLFKNTDHKEDKDELMIFITPKIIREGVNVY"
     gene            3012..3554
                     /locus_tag="VM713_05520"
     CDS             3012..3554
                     /locus_tag="VM713_05520"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014595709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HVH83760.1"
                     /translation="MLGKASVFLIGPMGSGKTAVGRSLARSLGVAFHDSDAEVERRTG
                     VDIPFIFEKEGEAGFRAREREAIEALTRLERIVLATGGGAVLSPENRRHLAERGVVVY
                     LETSVTQQADRVRHGRNRPLLDEGDIAGRLGELMDVRSPLYSGIADHTVSTDGRRVPA
                     VVEEILRLLARPPPAAPPGR"
     gene            3622..>4094
                     /locus_tag="VM713_05525"
     CDS             3622..>4094
                     /locus_tag="VM713_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HVH83761.1"
                     /translation="MDTLKVELGTRSYPILIGAGLLAQAEVLKPHVPGRDLLIVSNTT
                     VAPLYLPALAAGLPNQRRLEVILPDGEMHKTLENASRVLDVLISNRFGRDATVVALGG
                     GVVGDLTGFVAACYQRGVAFVQVPTTLLAQVDSAVGGETAVNHAGGKNLIGAFHQP"
BASE COUNT          710 a         1463 c         1313 g          608 t
ORIGIN      
        1 gggcgaggcc gagaagaagc agaaagacgt gacccaccgt ccgactccgc agccggccgg
       61 gtgaggtgta agtcatgagc caagtgaaac accgctgcgc gcttcgcccc ctgggtgcgg
      121 cgctcgcctg tctcggctac ctcctgatcg ccggctgctt gagcgccgac gacgacctca
      181 accgcttcat cgaggaaacc aagcacgagc cgggcggccg cgtcgagccg ctgcccgaga
      241 tcaagcccta cgagacctac gtgtatgcgg actcggagat gcgctcgccg ttcatgccga
      301 gcgcgccggg cgccggcgcg ggacttgccg gggtgcggcc cgactccaag cgcaaccgcg
      361 agttcctcga gcagttctcg ctcgacactc tgcggatggt cggcacgctg cgcctcggcg
      421 gcagcatgtt cggcctggta cagacgaagg acggcctggt gcatcgcgtc gcagtgggcg
      481 agcacttggg tcaggccgaa ggcaagatca ccgaaatctc accggccaag atcagcctcg
      541 ttgaaatcgt tccggacagc ctcggcgggt acatggagcg gcccgcggcg cttgccctga
      601 acgagtaatg gtccagcagg gagtcctcgc aatgcatgcc tctagcctct tgaagcgcat
      661 cctcggcacc gccgcctggg tcgtgatcgc gggggccttt gcggtcgccc cggttccgcc
      721 cgcgctggcc gcggatcaac cgagcctcca gtcggtggac gtgcagccgc tcgccggaca
      781 gcagctgcag ctcacgctac ggctgtcggg agcggcgccg gagccgctgt cgttcaccat
      841 cgacaacccg gcgcgcatct ccctcgacct gcccgggacg acactctcac tcccgtcgcg
      901 ccgcatcgac gtgcacgccg cgggactcga caccatcatc gcggctgaga cgaaggaccg
      961 cacccgcgtg gtactcaatc tcgacaagct cgtgccctac atcacgcgcg tcgaggggaa
     1021 cgacatcatc gtgctgctcg gcagcggcgc cgccggcacg gtcgctacca cccggggccc
     1081 gagcgtcgtc agcggcgctg gcgccgtggg ctctggcacc gtggccgcga gctccagcgg
     1141 cgttcgcgag ctgcgctcga tcgacttccg ccgcggcacc gacggtgccg gccgggtggt
     1201 cgtgaggctc tccgacccgc acatccgcat cagcctgaag caggtcggca gccagatcgt
     1261 ggtggacttc accgacgccg gagtgccggc gaacctcctg cgccgcttcg acgccaccga
     1321 ctacggcacc ccggtgaccg gcttcgacgt cagccgcgtc ggcaacggcg cgcgcatcgc
     1381 gatcaacgcc accggcgact acgagcagct cgcctaccag tccgacaacc agtacgtggt
     1441 cgaggtggcg ccgcgccgca aggcgaacgc cacggcggag gagaagcccg tctataccgg
     1501 cgagcgtctc acgctcaact tccaggacat cgacacccgc gcggtgctgc agctgctcgc
     1561 cgacgccagc ggccagaaca tcgtggtgag cgactcggtg agcggcaacg tgacactgcg
     1621 cctgcagaac gtgccgtggg accaggcgct cgacatcgtg ctgaagacca agggcctcga
     1681 caagcgccgc gacggcaacg tcatcatcgt cgccccgcag gccgagctcg ccacgcgcga
     1741 gaaggccgag ctcgccgccc gcaaggatgt ttccgagctc gcgccgctgc gctcggagta
     1801 cctgcaggtc aactacgcca aggcgcagga cctcgccgcg ctcatcaagt cgcagaacaa
     1861 ctcgctgctc tccccgcgcg gcagtgtcgc ggtcgatgag cgcaccaata cgctgttgat
     1921 gctcgacacg gccgacaagc tcgcggacat ccgccgcctg gtggcgaccc tcgacatccc
     1981 ggtgcgtcag gtgctgatcg agtcgcgcat cgtcatcgtg aacaacgact tcgagcggca
     2041 gatcggcgcg atcttcggca tcaccgcgtt ccaccagaac ggcagcacgc tctacaacac
     2101 caccggcacg gcggcgggca tcgacactca ggcgacgacc ttcatcaccg ggcagaacgc
     2161 gatcaacaac gcgatcaacg ccggcacgac gccggtgccg ttatcgacct acggcatccc
     2221 ggcggcgacc aaccgctaca acatcaacct gccggtaacc agtccggccg gctcgatcgc
     2281 gctcggcctg gtaaccaata acgtactggt cgacctcgag ctgaatgcgg cgcaggccga
     2341 gactcaggcg aacatcatct cctccccgcg cgtcatcacc gcgaaccaga aagaggcgac
     2401 gatcgagcag ggcatcgaga tcccctacca gcagtcggcc tcgagcggcg ccaccaccat
     2461 tcagttcaag aaggcggtgc tgcagctcaa ggtcaccccg cagatcaccc ccgacaaccg
     2521 catcattctt gacctcgacg tgcgcgacga ctcggtcggc accatcgtgg tcgcctcggg
     2581 cggcgtgaac gtgcccacca tcaacacgcg cgagatctcg acccaggtgc tggtgaacga
     2641 cggccagacc gtggtgctgg gggggattct gcagaccacg cagcgcgagg acgacaccaa
     2701 ggtgccgttc ctcggcgata tcccgatcct cggccacctg ttcaagaaca ctgatcacaa
     2761 agaggacaag gacgagctga tgatcttcat caccccgaag atcattcgtg aaggcgtcaa
     2821 cgtgtactga gatgtagctg ctgccagcgc ggccggatcc gggaatttat ccatccaggt
     2881 caagtcagcg gatacggcga cgcttacgac tctgctcgtt ccggtcacgg tcacctagac
     2941 tgcgattgct aagttgagcg cggcggccgc cttcgggcgg ccgcatcgtt ttcggtatcc
     3001 ttcgccacaa ggtgctcggc aaggccagcg tattcctcat cggccccatg ggctcgggca
     3061 agaccgccgt cgggcgctcg ctcgcgcgct cgctcggtgt ggcattccac gacagcgacg
     3121 ccgaggtcga gcggcgcacc ggcgtcgaca tcccctttat cttcgagaag gagggcgagg
     3181 cgggcttccg ggcgcgtgag cgtgaggcca tcgaggccct cacccggctc gagcgcatcg
     3241 tgcttgccac cggcggcggc gcggtgctct cgccggagaa tcgccggcat ctcgccgagc
     3301 gcggcgtagt cgtctacctc gagacctcgg tgacgcagca ggccgaccgc gtccgtcacg
     3361 gccgcaaccg cccgctgctc gatgagggtg acatcgccgg ccgtctcggg gagctcatgg
     3421 acgtgcgctc cccgctctac agcggaatcg ccgaccacac ggtctcgacc gacgggcggc
     3481 gcgtaccggc ggtcgtcgag gagatcctga ggctcctcgc acggccgccg ccggcggcgc
     3541 ccccggggcg ctgaagggac ggctcccggt atactgccgc gggcgcgaat cgcccgagat
     3601 ggctccgttc cgccgcgcgc agtggatacc ctcaaggtcg aactcggcac ccgcagctac
     3661 ccgatcctga tcggcgcggg gctgctcgcg caggctgagg tgctcaagcc ccacgtgccg
     3721 gggcgcgacc tgctcatcgt cagcaacacc accgtcgcgc cgctttacct gccggcgctc
     3781 gcggccggcc ttcccaacca gcgccgcctc gaggtgatcc tgccggacgg cgagatgcac
     3841 aagacgctcg agaatgcctc gcgggtgctc gacgtgctca tcagcaaccg cttcgggcgc
     3901 gacgcaaccg ttgtggcact cgggggcggc gtggtcgggg acctcaccgg cttcgtcgcg
     3961 gcctgctatc agcgcggcgt cgccttcgtg caggtgccga cgacgctcct tgcgcaggtg
     4021 gattcggccg tcggcggcga gaccgcggtc aaccacgcgg gcggcaagaa tctgatcggc
     4081 gccttccatc agcc
//
LOCUS       DATLKL010000419         1634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_207832, whole genome shotgun sequence.
ACCESSION   DATLKL010000419 DATLKL010000000
VERSION     DATLKL010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207832"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VM713_05530"
     CDS             <1..393
                     /locus_tag="VM713_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83762.1"
                     /translation="REACTAYLGRVLDWFDGRDERLGELCARPPVGDAGRSALAALAV
                     PGRGREALAGLEAAESASESWQALAQFINGPWDARRGAIEASLAAIARVADWRAFGRV
                     DADSIWQERALYARFRAALPAGTPPGLV"
     gene            458..1012
                     /locus_tag="VM713_05535"
     CDS             458..1012
                     /locus_tag="VM713_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HVH83763.1"
                     /translation="MAAGASTRFGSPKQLVRIGGRPLLHAVVTRAAEVTGNALIVVLG
                     AGAAELAPLLKHSPGSVVVNRHWREGLASSIRAGVARLPATCAGVMLLLADQAAVTSD
                     DLRRLAGTWRRRPQCIVAALYAGTTGAPAIFPRSAFAELAALRGDSGARSVLRRSADR
                     VIRVPMPGAELDIDTPEDLLQIDS"
     gene            complement(1049..>1634)
                     /locus_tag="VM713_05540"
     CDS             complement(1049..>1634)
                     /locus_tag="VM713_05540"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HVH83764.1"
                     /translation="LTTLETERGMSRGATGLLVIATETPRGMLSLPLYPTALGAARAA
                     TVRLAGLTWGAEDLGAALGAAAKHDSSGALTFTFALARTTCLLAAAALGVQAIDGVHV
                     DFRDASGLERALVEARRDGFSGKLAIHPDQVGPINAAFTPTEEERQHARRVVAAFAAA
                     GGAGVASLDGQMIDRPHLLQARRVLAASAPARKP"
BASE COUNT          212 a          593 c          595 g          234 t
ORIGIN      
        1 cgtgaagcct gcaccgcgta cctcgggcgc gtgctcgact ggttcgacgg gcgcgacgag
       61 cgcctcggcg agctctgcgc ccgaccgccc gtcggcgacg ctggccgcag cgctctcgcg
      121 gcgctcgccg tccccggccg aggacgcgag gcgctcgcgg ggctcgaggc ggcggaatct
      181 gcgagcgaga gctggcaggc actggcgcaa ttcatcaacg ggccatggga tgcccggcgc
      241 ggcgccatcg aggcgtcgct cgccgcgatt gcgcgcgtag cggactggcg cgcgttcggc
      301 cgagtcgacg ccgactccat ctggcaggag cgcgcgctct atgcgcgctt tcgcgcggcc
      361 ctccccgcgg gcacaccgcc ggggctggtg taggagcggc gcggtgctcg agccctaacc
      421 cccagggaaa gccggcgacc gcctctacgc aatcgtactg gctgcagggg cttcgacccg
      481 cttcggctcg ccgaagcagc tcgtgcgcat cggcgggcgt ccactgctgc acgccgtcgt
      541 gacgcgtgcc gcggaggtca ccggcaacgc cctcatcgtc gtcctgggcg caggcgcggc
      601 tgagctcgcg ccgctactca agcactcacc cggctcggtc gtggtcaacc gtcattggcg
      661 cgaggggctc gcgagctcga tccgcgcggg agtcgcacgg ctgcccgcca cgtgtgccgg
      721 tgtgatgctg ctgctggccg atcaggccgc ggtcaccagc gatgatctcc ggcgcctcgc
      781 cggtacctgg cgccggcgtc cgcaatgcat cgtcgccgcg ctctatgcgg gcacgaccgg
      841 tgccccggcg atcttcccgc gcagcgcctt cgctgagctc gccgcgttgc gcggcgacag
      901 cggcgcgcgc tcggtcctgc gccgcagcgc ggatcgcgtg atccgcgtgc ccatgccagg
      961 cgcggagctc gatatcgaca cgcccgagga tctgctgcag atcgatagtt gatctggtcg
     1021 aaatcgcagg aagcgatttc cggctgggtc agggcttgcg cgccggcgcg ctcgcggcga
     1081 gtacccgccg cgcctgcagg agatgcggtc gatcgatcat ctgtccgtcg aggctcgcaa
     1141 cccccgcgcc gcccgcggcg gcgaaggccg cgaccacgcg ccgggcatgc tggcgctctt
     1201 cttcggtggg agtgaaggcg gcattgatcg gcccgacctg gtcggggtgg atggcgagct
     1261 tgcccgagaa accgtcgcgg cgcgcctcga cgagcgcgcg ctcgaggccg ctcgcatcgc
     1321 gaaagtcgac atggacgccg tcgatggcct gcacaccgag ggccgcggcc gcaagcagac
     1381 aggtggtgcg cgccagcgcg aaggtgaaag tcagcgctcc cgaggagtcg tgcttcgcgg
     1441 ccgcgccgag cgccgcgccc aggtcctcgg ctccccaggt gagaccggcg agccgcaccg
     1501 ttgccgcgcg cgcggcgccg agcgcggtcg gatacagcgg cagcgacagc attccccgcg
     1561 gtgtctcggt ggcgatgacc agcaatcccg tcgccccgcg gctcatgccg cgttccgtct
     1621 cgagcgtcgt gaga
//
LOCUS       DATLKL010000420         1631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_196638, whole genome shotgun sequence.
ACCESSION   DATLKL010000420 DATLKL010000000
VERSION     DATLKL010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196638"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /gene="rpsG"
                     /locus_tag="VM713_05545"
     CDS             <1..368
                     /gene="rpsG"
                     /locus_tag="VM713_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HVH83765.1"
                     /translation="KQVAQRIVYTALEGTREKTGTDPVVTLKRALDNVKPAIEVKSRR
                     VGGATYQVPVEVRATRRQTLAMRWVIEAARDRSEKSMAFRLAHELMDASENRGAAVRK
                     REDTHRMAEANKAFAHYRW"
     gene            391..>1631
                     /gene="fusA"
                     /locus_tag="VM713_05550"
     CDS             391..>1631
                     /gene="fusA"
                     /locus_tag="VM713_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HVH83766.1"
                     /translation="MARATPIERYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAAVMDYMEQEQERGITITAAATTCFWKGMDNSFPEHRINILDTPGHVDFTIEVERS
                     LRVLDSAVALFDSVAGVQPQSETVWRQMNKYRVPRIAFVNKMDRAGANFLRVVEMIRA
                     RLRANPVAIQLPIGAEDSFEGVIDLIRMKAIYWDMETQGMKFEYQEIPQHLRAQAEEY
                     RAKMIEAAAEANDTLMHKYLEHEQLSDAELRAGLRERSLRNEVVLAMCGSAFKNKGVQ
                     ALLDAVIEFMPSPVDMPDVKGIDDHERPITRKASDEEPFSALAFKILNDPFVGNLTFF
                     RVYSGVLNSGDTVFVPTKGRKERIGRLLQMHASERTEIKEVRAGDIAAAVGLKDVTTG
                     DTLCAQDHVITLERMTFPEPV"
BASE COUNT          320 a          546 c          529 g          236 t
ORIGIN      
        1 agaagcaggt cgcccagcgg atcgtctaca ccgctctcga aggcacccgt gagaagaccg
       61 gcaccgaccc ggtcgtcacg ctcaagcgcg ctctcgacaa cgtgaagccc gccatcgagg
      121 tcaagtcccg ccgcgtcggc ggcgcgacct accaggtgcc ggtcgaagtg cgcgccacgc
      181 gccgccagac cctcgccatg cgttgggtca tcgaggcggc gcgcgatcgc agcgagaagt
      241 cgatggcgtt ccgcctggcg cacgagctga tggatgcctc cgagaaccgc ggcgccgcag
      301 tgcgcaagcg cgaggacacg caccgcatgg cggaggccaa caaggccttc gctcactacc
      361 gctggtaaga gctcacgaag gaatattgca gtggcacgcg cgacgcccat cgagcgctat
      421 cggaacatcg gcatctccgc gcacatcgac gcggggaaga cgaccacgac cgagcgcatc
      481 cttttctaca ccggcgtctc gcacaagatc ggcgaggtcc acgacggcgc ggccgtcatg
      541 gactacatgg agcaggaaca ggagcgcggc atcaccatca ccgccgccgc cacgacctgc
      601 ttctggaagg ggatggacaa cagcttcccc gagcaccgca tcaacatcct cgacaccccg
      661 ggacacgtcg acttcaccat cgaggtcgag cgttctctgc gggtgctcga ttcggcggta
      721 gcgctctttg actcggtcgc cggcgtgcaa ccgcagtccg agaccgtatg gcggcagatg
      781 aacaagtacc gtgtgccgcg catcgccttc gtcaacaaga tggaccgcgc cggagctaat
      841 ttcctgcgcg tcgtcgagat gatccgcgcg cggctgcgcg ccaacccagt cgcgatccag
      901 ctgccgatcg gcgccgagga ctcgttcgag ggcgtcatcg acctgatccg catgaaggcg
      961 atctactggg acatggaaac ccaggggatg aagttcgagt accaggagat cccgcagcac
     1021 ctgcgggcgc aggccgagga gtaccgcgcc aagatgatcg aggcggccgc cgaggccaac
     1081 gacacgctga tgcacaagta cctcgagcac gagcagctct cggatgcgga gctgcgtgcg
     1141 gggctgcgcg agcgctcgct gcgcaacgag gtggtgctgg cgatgtgcgg ctcggccttc
     1201 aagaacaagg gcgtgcaggc cctgctcgat gccgtcatcg agttcatgcc ctcaccggtc
     1261 gacatgccgg acgtgaaggg catcgacgac cacgagcgcc cgatcacccg caaggcgagc
     1321 gacgaggagc cgttctccgc gctcgcgttc aagatcctca atgatccctt cgtcggtaac
     1381 ctgaccttct tccgcgtcta ctcgggggtc ctcaactccg gcgatacggt gttcgtgccg
     1441 accaagggcc ggaaagagcg catcggacgg ctgctgcaga tgcacgcgag cgagcgcacg
     1501 gagatcaagg aggtgcgcgc cggcgacatc gccgcggcgg tgggcctgaa ggacgtcacc
     1561 accggcgaca cgctgtgcgc gcaggaccac gtcatcaccc tggagcgcat gacgttcccg
     1621 gagccggtga t
//
LOCUS       DATLKL010000421         4317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_129147, whole genome shotgun sequence.
ACCESSION   DATLKL010000421 DATLKL010000000
VERSION     DATLKL010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129147"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VM713_05555"
     CDS             complement(<1..397)
                     /locus_tag="VM713_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH83767.1"
                     /translation="MTTQPQVDRAAILTRRARVLSPTYRLFYDQPLQIVRAEGVWMYD
                     EHGRAYLDAYNNVPVVGHCHPYVVEALTRQARTLNTHTRYLAEQPLQLAEELLGSMPP
                     ELANIVFTCTGSEANDLAVRVSKAVTGGTD"
     gene            complement(394..1437)
                     /locus_tag="VM713_05560"
     CDS             complement(394..1437)
                     /locus_tag="VM713_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HVH83768.1"
                     /translation="MATDVEDFMATMVAPPRPVPLERALALVRESYGLEPQSTARLTG
                     ERDENFRLTAAGDAEYVLKIANPAENPAETDFQTAALLHVEETDPALPCPRVLRNRAG
                     NTHIRFLDEGGAGRTARVLTFLPGRLLGASTRSRQQRAACGRIGARLTLALRDFEHPA
                     ARRTIVWDVRHTGHMRRLLEELPRFPYHGAARELLAKLTPTIESQLPRLRHQVVHGDL
                     NPLNILVDPADEERVTGVIDFGDMTHTALIADVAVCAAELVPIDCADAGAARDAILDV
                     AGAYHACVPLLRPELALLGALVAARLLQTLVIHEWHVQRNPASGHYQPLAEDFMRARL
                     RIADRLLLEEMRL"
     gene            complement(1484..2509)
                     /locus_tag="VM713_05565"
     CDS             complement(1484..2509)
                     /locus_tag="VM713_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVH83769.1"
                     /translation="MTSNVSGGRVPLALSCACLLLGMQGVARAAPGDEVYTRPGRLIP
                     AAGTRLNFYCMGSGRPAVVFDSGWEDWAPVWTLVQPEVAKWTRACSYDRAGAGFSDPG
                     PLPRTSVRIADELHGALHHAGIDGPYILVGHAFGGDNVRTFAQRYATEVAGLVLVEAD
                     VGGLDDHRGDAGHIASLRDCREAIASGKPLPRLPARPGRPDRSCAQQFFRGLPEATWS
                     PQLNAKLLELAQTKLAMYEAYISEMEQMPADEAYLEQHRRALGSRPVRVLSTGYHGVH
                     TMDPAHPLDAQQQQYEQQVARAQAKWLDLSSNSKQLFTDQSSEYIPFDQPGFVIDAIR
                     EVYSQSR"
     gene            complement(2499..3854)
                     /locus_tag="VM713_05570"
     CDS             complement(2499..3854)
                     /locus_tag="VM713_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVH83770.1"
                     /translation="MRNSLAVSLLLALLGATQAAAPAAAPAVLVKAARLIDPRSGSAL
                     SPAAVLIEDGKVKLVGPPARVQADTPATVKVVDLGGATLLPGLIDGHTHLLLDVIVPQ
                     EAQVKRMTFNGEFEPGLLLAIAGMTPSARVLLGAQLAREDLESGFTTVRNVGHSGIDG
                     DVALRDAINAGRVAGPRMLASGRKIIASGDYIESLNPAVAPSIAEQEFLFLDGADSAR
                     RAVRANAFYGVDLIKVALDDNISLAEMLALVDEAHRQQLKVAVHAPSALSIQIAIDAG
                     ADSIEHGNEASDEQLRAMRAKGIFLDLTPTVFDGTFWARVHSTTVLSADFRAQLLARD
                     ARGRERAAALVQRVLRSGVKFAAGSDMCWYLPGKTRGEAAATMFTALRTAGMPPLDIL
                     RAVTTNAAEMLGWQDRVGAVEPGKLADLIAVAGDPLADISELERVRFVMKGGQVIRND
                     L"
     gene            3918..4316
                     /locus_tag="VM713_05575"
     CDS             3918..4316
                     /locus_tag="VM713_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HVH83771.1"
                     /translation="MKRTRLSKAEARILELYWKLGTRSVREILDSLPEEERVAYTTVQ
                     TLVYRLEQKGALRRVRKIGNAQLFEPAIDQREFRTGLVRDLLDLFGGSPRLLVSNLLE
                     TGTITLKDLKALQQALPPKAPRRGRGKGHA"
BASE COUNT          674 a         1432 c         1515 g          696 t
ORIGIN      
        1 gatctgtacc gcccgtcacc gctttgctga cgcgtaccgc gagatcgttg gcctcgctcc
       61 ccgtgcaggt gaagacgatg ttagcgagct cgggcggcat gctgccgagc agctcctcgg
      121 cgagctgcag cggctgctcg gcgagatagc gggtgtgggt gttgagcgtg cgcgcctggc
      181 gcgtcagtgc ctcgaccacg taggggtggc agtggcccac caccgggacg ttgttgtagg
      241 cgtcgagata ggcgcggccg tgctcgtcgt acatccatac gccctcggca cgcacgatct
      301 gcagcggctg gtcgtagaag agccggtagg tgggggagag cacccgcgcg cggcgtgtca
      361 ggatcgccgc gcggtccacc tgcggttgag tcgtcacagc ctcatctcct cgagcaacag
      421 tcggtcggcg atgcgcagtc gggcgcgcat gaaatcctca gcaagcggct ggtagtggcc
      481 gctcgcgggg ttgcgctgca cgtgccattc gtgaatgacc agggtctgga gcagccgcgc
      541 cgccaccagc gcgccgagca gcgccagctc gggccgcagc agcggcacgc acgcatgata
      601 ggcgccggcg acatcgagga tggcgtcgcg cgccgccccc gcatccgcgc aatcgatcgg
      661 gacgagctcg gcggcgcaca ccgccacgtc cgcgatcaga gccgtgtggg tcatgtcgcc
      721 gaagtcgatc accccggtta cgcgctcctc gtcggcgggg tccaccagaa tgttgagggg
      781 attcaggtcg ccgtgcacga cctgatgtcg caggcgcggc agctgtgatt cgatcgtcgg
      841 ggtgagcttc gcgagcagct cgcgagccgc cccgtggtac gggaagcgcg gcagctcctc
      901 gaggagtcgc cgcatgtgcc ccgtgtggcg cacgtcccag acgatcgtgc gcctggccgc
      961 cgggtgctcg aagtcacgca gcgcgagcgt gaggcgcgcg ccaatccggc cgcaggcggc
     1021 gcgctgctgg cgcgaacggg tagacgcgcc gagcagccgg cccgggagaa aggtcaggac
     1081 ccgggccgtg cgtccggcgc cgccctcgtc caggaaacgg atgtgagtgt tcccggcacg
     1141 gttgcgcagc acgcgcgggc acggcagggc cggatcggtt tcctcgacgt ggagaagtgc
     1201 cgcggtctga aagtcggtct cggccggatt ctcggccggg ttcgcgatct tgagcacgta
     1261 ttccgcgtcg ccggcggcgg taagcctgaa gttctcatcg cgctcgcccg tgagccgtgc
     1321 ggtcgactgc ggctcgaggc cgtagctctc ccgcaccagg gccagggcgc gctcgagcgg
     1381 caccggccgc ggcggtgcga ccatggtggc catgaaatcc tcgacgtcgg tggccacagt
     1441 gcagctcccc ggggctagcc cagggggtgg gttttactgt ttcctacctg ctttgtgaat
     1501 acacctcgcg gatggcatcg atcacgaagc cgggctggtc gaagggaatg tattcggagc
     1561 tctggtccgt gaacagctgc ttggagttcg aggacaaatc gagccatttc gcctgcgcgc
     1621 gcgccacctg ctgctcgtac tgctgctgct gcgcatccag cggatgcgca ggatccatcg
     1681 tatgcacccc gtgatagccg gtcgagagca cccggacggg gcgcgagccg agggcgcggc
     1741 ggtgctgctc gaggtaggcc tcgtctgcgg gcatctgctc catctcggag atataagcct
     1801 cgtacatcgc gagtttcgtc tgtgcaagct cgaggagctt ggcgttgagt tgcggcgacc
     1861 aggtggcctc gggcaagccg cggaagaatt gctgcgcgca gctgcggtcc gggcggccgg
     1921 gacgggcggg gagccgcggc agcggcttgc cggaggcgat cgcctcgcga cagtcgcgta
     1981 gcgatgcgat gtggccggcg tctccccggt ggtcgtcgag cccgccgacg tcggcctcca
     2041 ccagcacgag tccggcaacc tcggtcgcgt accgctgcgc aaaggtacgt acgttatcgc
     2101 cgccgaaagc atggccgacc aggatgtagg gcccgtcgat cccggcatgg tgcagcgcgc
     2161 cgtgcagctc atcggcgatg cgcacgctgg tgcgcggcag cgggcccgga tcgctgaagc
     2221 ctgcaccggc gcgatcgtaa ctgcaggcgc gcgtccattt ggctacctcc ggctgcacaa
     2281 gcgtccacac cggtgcccag tcctcccacc cggaatcgaa gaccaccgcg ggccggccgc
     2341 tccccatgca atagaaattg aggcgcgtcc cagcggccgg gatgagtcgt ccgggccgcg
     2401 tgtagacctc atcccccggc gcggcgcgtg ccacgccctg catcccgagc agcaggcagg
     2461 cacagctcag cgccagcggt acccgcccgc ccgacacgtt agaggtcatt gcggatcacc
     2521 tgaccgcctt tcatgacgaa gcggacgcgt tcgagctcgc tgatgtcggc gagcgggtct
     2581 cctgcgacgg cgatgagatc ggcgagcttg ccgggctcga cggctcccac gcggtcttgc
     2641 cagccgagca tctcggcggc gttggtcgtg acggcgcgca ggatatcgag cggcggcatt
     2701 ccggcggtgc gcagcgccgt gaacatggtt gcggcggcct cgccgcgcgt cttgcccgga
     2761 aggtaccagc acatgtcgga gccggcggcg aatttcaccc cggacctcag cacccgctgc
     2821 accagcgcgg ccgcgcgctc gcggccgcgc gcgtcacgag cgagcagctg ggcgcgaaag
     2881 tccgccgaca gcacggtggt cgagtgaacc ctcgcccaga aggtgccgtc gaagacggtc
     2941 ggcgtcaggt cgaggaagat gcccttggcg cgcatcgccc gcagctgctc gtcgctcgct
     3001 tcgttgccgt gctcgatcga gtcggcgccc gcatcgatcg cgatctggat gctcagcgcg
     3061 ctgggagcgt gcaccgcgac cttgagctgc tggcgatgcg cttcgtcgac gagcgccagc
     3121 atttccgcga gactgatatt gtcatcgagc gcgaccttga tgagatccac cccgtagaag
     3181 gcattggcgc gcacggcacg tcgcgccgag tcggcgccgt cgaggaacag gaactcctgc
     3241 tccgctatcg aaggcgcgac ggcggggttg agactctcga tgtagtctcc ggaggcgatg
     3301 atcttgcgtc ccgaggccag catgcgtggt cccgctacgc ggccggcgtt gatggcgtcg
     3361 cgcagggcga cgtcgccgtc gattccggaa tggcccacgt tgcgcaccgt agtaaagcca
     3421 ctctcgagat cctcgcgggc gagctgcgca ccgaggagca cgcgggcgct cggtgtcatc
     3481 ccggcgatcg cgagcagcag ccccggctcg aactcgccgt taaaggtcat gcgcttcacc
     3541 tgcgcttcct gcggcacgat cacatcgagc agcaggtgtg tatggccatc gatcaggccg
     3601 gggagtagcg tggcgccgcc gagatcgacg accttcaccg tggcgggagt gtccgcctgc
     3661 actcgggcgg gcgggcccac cagcttgacc ttgccgtcct cgatcagcac ggcagctgga
     3721 gacaaggcgc tgccgctgcg cggatcgatc agccgggccg ccttgaccag caccgcgggc
     3781 gcggcagcag gcgctgcggc ctgagtggct cccaggagcg ccaggagcag gctaaccgcc
     3841 agggaattac gcatctctcg cccctccgtt gccgtcctac gttatatgta gtagcattta
     3901 tttgaggcgt agtaaatatg aagcgcaccc gtctctccaa ggccgaggcc cgcatcctgg
     3961 agctttactg gaagctggga acgcgctcgg tacgcgagat cctcgatagc ctccccgagg
     4021 aggagcgggt cgcgtacacc accgtgcaga cgctggtcta ccggctcgag cagaagggtg
     4081 ccctgcgccg ggtccgcaag ataggcaacg cgcagctgtt cgagcctgcc atcgaccagc
     4141 gcgagttccg caccggcctg gtgcgcgatc tgctcgacct gttcggcggg tccccgcgcc
     4201 tcctggtctc aaatctcctc gagacgggta cgattacgct caaggacctc aaggccctgc
     4261 agcaggcgct gcccccgaag gctccgcggc gcgggcgagg taagggtcat gcatgag
//
LOCUS       DATLKL010000422         1820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_207923, whole genome shotgun sequence.
ACCESSION   DATLKL010000422 DATLKL010000000
VERSION     DATLKL010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207923"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /gene="hemH"
                     /locus_tag="VM713_05580"
     CDS             <1..668
                     /gene="hemH"
                     /locus_tag="VM713_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HVH83772.1"
                     /translation="QRILVVPLFPQYCGATTGAVYDQVSEELRRWRWLPELRFVAEYH
                     DHPGYIEALRASVAEHWSREGRTGHLLMSFHGIPERYFADGDPYFCKCQKTARLLADE
                     LMLRESEWSVAFQSRFGPSAWLKPYTRAVLEGMPARGARSVTVLCPGFAVDCLETLEE
                     IAIENRASFMRAGGERFQYVPALNARAPHAQCLGDLIVEHTHGWTHPEHGRLPSAARG
                     ASA"
     gene            668..1399
                     /locus_tag="VM713_05585"
     CDS             668..1399
                     /locus_tag="VM713_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVH83773.1"
                     /translation="MARAPLPPPGRFHELSLATDDIRASVEFYERLGFSQTVTADTYA
                     HPYGVLTDGRVHIGLHQRRGVSPTLTFVRPGIAACLAEFAALGIELTLCRTGEDVFNE
                     IGFAGPHGESVRVIEARTYSPAARSSAEVSLCGDFAQLSLPATDFAAARTFWEPLGFV
                     ATDEVAAPYEHLPLTSDHLDLAFHRPRTCDAPLLVFRAADMAQRIERLRTLGVAEEAS
                     PRGIARGTLLASPEGTLLLLLSDSD"
     gene            1470..>1820
                     /locus_tag="VM713_05590"
     CDS             1470..>1820
                     /locus_tag="VM713_05590"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HVH83774.1"
                     /translation="MRALPALTALAVLATSAAYGDPGMWTFHDFPSERVMKEHGVVID
                     QAWLDRVRTATIRLANCTASFVSPDGLVLTNHHCSESCLDEHSTAEQPLLKNGFLARK
                     REQELRCGTQVADVL"
BASE COUNT          262 a          674 c          592 g          292 t
ORIGIN      
        1 cacagcgcat cctcgtggtg ccgctgtttc cgcagtactg cggcgccacc accggcgcgg
       61 tctacgatca ggtgagcgag gagctgcgcc gctggcgctg gctgccggag ctgcgcttcg
      121 tcgccgagta ccacgatcat cccggctaca tcgaggcgct gcgcgcgagc gtcgccgagc
      181 actggagccg cgaggggcgt accgggcacc tgctgatgtc gtttcacggc attcccgagc
      241 gctacttcgc ggacggcgac ccgtatttct gcaagtgcca gaagacggcg cggctgctgg
      301 cggacgagct gatgctgcgc gagagcgagt ggagcgtcgc cttccagtcg cgcttcgggc
      361 cgagcgcctg gcttaagccc tatacgcgtg cggtgctcga gggcatgccg gcgcggggcg
      421 cgcgcagcgt gacggtgctg tgcccgggct tcgcggtcga ctgcctggag acgctcgaag
      481 agatcgccat cgagaaccgc gcgtccttca tgcgcgccgg cggcgagcgc tttcagtacg
      541 tgccggcgct caacgcccgc gctccgcacg cgcagtgcct cggcgatctg atcgtcgagc
      601 atacccacgg ctggacacac cccgagcacg gacggctgcc gagcgccgcg cgcggcgcct
      661 ccgcctgatg gcgcgtgcgc cgctgccgcc gcccgggcgt tttcacgagc tgagcctcgc
      721 gaccgacgac atccgcgcct cggtcgagtt ctacgagcgg ctcggcttct cccagaccgt
      781 gaccgccgac acctacgcgc atccttacgg cgtgctcacc gacgggcggg tccacatcgg
      841 cctgcaccag cgacgcggcg tctcgcccac gctgaccttc gtgcggcccg gcattgccgc
      901 ctgcctggcc gagttcgccg cgctcggtat cgagctcacc ctctgccgca ccggcgagga
      961 cgtcttcaac gagatcggtt tcgcgggccc gcacggggag tccgtgcggg tgatcgaggc
     1021 gcgcacctac tcgcccgccg cgcgctccag cgccgaggtc tcgctgtgcg gcgacttcgc
     1081 gcaactgtcg cttccggcga cggacttcgc cgcggcccgc accttctggg agccgctcgg
     1141 cttcgtcgcg accgacgaag tggcggctcc ctacgagcat ctgccgctta ccagcgatca
     1201 cctcgatctc gccttccacc gtccgcgcac ctgcgacgcg ccgctgctcg tgttccgcgc
     1261 ggcggacatg gcacagcgga tcgagcggct gcgcacgctc ggcgtcgcgg aggaggcgtc
     1321 gccgcgcggc atcgcccgcg ggacgctgct cgcaagcccc gagggcacac tgctgctgct
     1381 gttgtcggat tccgactgat cgaatcgcgg gtaatggcgc cccgaccaaa cgccaataat
     1441 cacaatcgcc aataatcaaa ggaccgccca tgcgcgccct cccggccctg accgcactcg
     1501 ccgtgctcgc gacctcagcc gcctacggcg accccggcat gtggactttt catgacttcc
     1561 cgagcgagcg cgtgatgaaa gagcatggcg tcgtcatcga ccaggcgtgg ctcgaccggg
     1621 tacgcaccgc gaccattcgc ctcgccaact gcaccgcctc attcgtgtcc cccgacgggc
     1681 tggtcctcac caaccaccac tgttcggagt cgtgtctcga tgagcactcc accgccgagc
     1741 agccgcttct gaagaacggc ttcctcgcgc gcaagcgcga acaggagctg cgctgcggaa
     1801 cgcaggtcgc ggatgtgctt
//
LOCUS       DATLKL010000423         3007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_38822, whole genome shotgun sequence.
ACCESSION   DATLKL010000423 DATLKL010000000
VERSION     DATLKL010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38822"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VM713_05595"
     CDS             complement(<1..970)
                     /locus_tag="VM713_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HVH83775.1"
                     /translation="MSGPYKRLETKLVHAGEPRPRIDGAVETPIFQSATYEYRGERRY
                     DDVRYLRLNNTPNQLALHAKLADLEGSEAALVTASGMAAITTTLLTVLSAGDHLLAQS
                     CLYGGTHDFVTREFAGLGLSVDFIDADRRESWRAQLKPNTRAVYVEAMTNPLLEVADL
                     RAVTEFARARGLVSIIDNTFASPVNFRPIEAGFDLVLHSATKYLNGHSDIVAGAVAGS
                     AALIERIRHKANHLGGSLDPHAAFLLSRGLKTLALRVRYQNGSALRIAQFLEAHPAVA
                     RVHYAGLPSHPRHERAAGLFAGFGGVLSFELKGPAARADELAARVRI"
     gene            1122..1886
                     /locus_tag="VM713_05600"
     CDS             1122..1886
                     /locus_tag="VM713_05600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVH83776.1"
                     /translation="MTRPTERFSSRVDNYARYRPSYPPPAIELLRERCGLIPGAVVAD
                     LGSGTGILTQLLLERGAEVFAVEPNDGMRAAAEAQLGRYPRFRSIRGSAEDTTLAPAS
                     VELLVAGQAFHWFDPQRARTEALRVLRPGAWAALLWNERPPEATAFLADYEALLRRQA
                     PEYTRITASRADVSTMRQFLGEAMEVRAFPNQQVLDFEGLKGRLMSSSYAPEPGSAAY
                     EPMIALLRGVFDRHERDGRIVMPYSSLVYFAQLRGS"
     gene            complement(1903..2559)
                     /locus_tag="VM713_05605"
                     /pseudo
     CDS             complement(1903..2559)
                     /locus_tag="VM713_05605"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
     gene            complement(2559..>3007)
                     /locus_tag="VM713_05610"
     CDS             complement(2559..>3007)
                     /locus_tag="VM713_05610"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HVH83777.1"
                     /translation="ADGRLRNWWTPADFAHFEAAGKRLAEEFDRYHPFADVAVNGRQT
                     LSENIADVAGLSDSFDAWRLTLGGKQAAGRAGFTAEQLFFLSFAQAWREKIREAALRQ
                     QLVTDGHAPHEYRGDTVRNLDAWYDAFGIKAGEKLYLAPSDRVRVW"
BASE COUNT          454 a         1019 c         1046 g          488 t
ORIGIN      
        1 gaatccgtac gcgggctgcc agctcgtccg cccgcgcggc cggacccttg agctcgaagc
       61 tcagcacgcc gccgaagccg gcgaacaggc cagcggcgcg ctcgtgccgc gggtggctcg
      121 ggagccccgc atagtgcacg cgggccacag cggggtgcgc ctcgagaaac tgtgcgatcc
      181 gcagcgcgct cccgttctgg tagcgcacgc gcagcgccag cgtcttcagc ccgcggctga
      241 ggaggaaggc ggcgtgcgga tcgagcgagc cgcccagatg gttggccttg tggcggatcc
      301 gctcgatcag cgcggcgctg ccggcgaccg cgcccgcaac gatgtcggag tgcccgttca
      361 gatacttggt ggcgctgtgg agaaccaggt cgaagccggc ctcgatggga cggaagttga
      421 ccgggctcgc aaaggtattg tcgatgatgg agacgaggcc gcgcgcgcgg gcgaactcgg
      481 tcaccgccct caagtccgcc acctcgagca gcgggttggt cattgcctcg acgtacacgg
      541 cgcgcgtgtt gggcttcagc tgcgcgcgcc agctctcgcg ccggtcggcg tcgatgaagt
      601 ccaccgacag accgagaccc gcaaactcgc gtgtcacgaa gtcatgcgta ccgccgtaca
      661 ggcagctctg ggccagcagg tgatcgccgg ccgacagcac cgtcagcagt gtcgtggtga
      721 tcgccgccat gccgctcgct gtgaccagcg cggcctcgga cccttcgagg tcggcgagct
      781 tggcgtgcag cgcgagctgg ttgggcgtgt tgttgagtcg caggtagcgc acgtcgtcgt
      841 aacgccgctc gccgcggtac tcgtaggtgg ccgactggaa gatcggtgtt tccacggctc
      901 cgtcgatgcg cgggcgcggc tctccggcat gcaccagctt ggtctcgagg cgcttgtagg
      961 ggccggacat cgcgatcctc cgcacgacgc tgtgacgaca ttgtcacgcg attaagctac
     1021 cgtgtccgca tgtctttatg gagaggcgcg cagcgggacc tgagcgcaca ctgtgtcgtg
     1081 gcgaggccag tacagtgtcc gcgcctgccg caagactcaa catgacgcga ccgaccgagc
     1141 gcttcagctc ccgggtagac aactacgcgc gctaccgccc gagctatccg ccgccggcga
     1201 tcgagctgct gcgggagcgc tgcggactca ttcccggcgc cgtggttgcc gatctcggct
     1261 ccggcaccgg catcctgacg cagctgctcc tcgagcgcgg tgcggaggtc tttgcggtcg
     1321 agccgaacga tggcatgcgt gccgccgcgg aagctcagct cggccgctac ccgcgctttc
     1381 gcagcattcg tggcagcgcc gaagacacca cgctcgcgcc cgcgagcgtc gagctcctgg
     1441 tcgcgggcca ggcctttcac tggttcgatc cgcagcgcgc gcggaccgag gcgctgcgcg
     1501 tgctgcgccc gggcgcgtgg gccgcgcttc tctggaacga gcgcccgccc gaggcgacgg
     1561 cgtttctcgc cgactatgag gccctgctgc gccgccaggc gcccgaatac acgcgcatca
     1621 ccgcgagccg cgccgacgtc agcaccatgc ggcagttcct cggcgaggcg atggaggtca
     1681 gagccttccc gaatcagcag gtgctcgatt tcgagggact gaagggacgg ctgatgtcct
     1741 cgtcctatgc acccgagccc gggtctgcgg cctatgagcc catgatcgcg ctgctgcgcg
     1801 gggtgttcga ccgtcacgag cgcgacggcc gcatcgtcat gccctacagc agcctggtct
     1861 attttgcgca gctgcggggc tcgtgaacgg ccgccgccgc acctactggg gaacgcgctt
     1921 cttcagctcc tcgagctcca cacgcaactg gcgcagctcc tcctcgagcc gctcgagccg
     1981 cgcgccggtg tccggcgcat ccggcgcagc gacaacggga ggctctgccg tgggtggcgc
     2041 tatcgccacc ggaccgctga agaggtgcgc atagcgcgaa tcgcggcggc cgggctcccg
     2101 cggcaactgc acgacgaacg gcccgtcgtc gcgcgtgcgg agcccctcga gcgtgccctc
     2161 cgcctcggtc acgtcggcga agggcgccat gcggctcgcg cgggcgcgca gttctcccgg
     2221 ggtctgcggc ccgcgcagca tgagctcgca tacgatcgcg agctcgggcg cggagagctt
     2281 gagagtgccg aactcggtgt tgcagaagcg gtgctggtat tacggcaccc ggctgccgaa
     2341 gcctgacttc tcgatcacca gatgccgccg cgcgagttga tcgagggtgt tctgcaccga
     2401 cgcctcgtcg agatccatga cagggtgacg attgctcttc tggttgcagg ccgcaaccaa
     2461 ggcattgagc gagagcggat actggtcagg cgtggtgatc tgcttctcga tcagatagcc
     2521 gatgacgcgg gcctcgaggg gcgagagctc gatcttcatt accagacccg cacgcggtcg
     2581 gacggggcga gatagagctt ctcgcccgcc ttgatgccga aggcgtcgta ccaggcgtcg
     2641 aggttgcgca cggtgtcgcc gcggtactcg tgcggggcat gtccgtcggt gaccagctgc
     2701 tggcgcagcg cggcctcacg gatcttctcg cgccaggctt gcgcgaagct gaggaaaaac
     2761 agctgttcgg cagtgaaacc cgcgcggccc gcggcctgct tgccgccgag ggtcaaccgc
     2821 caggcgtcga acgagtccga taaccccgct acgtcggcga tgttctcgct gagcgtctgc
     2881 cggccgttca ccgcaacgtc cgcgaacggg tggtagcgat cgaactcttc ggcgaggcgc
     2941 ttgcccgcgg cctcgaagtg cgcgaagtcc gccggggtcc accagttgcg cagccgcccg
     3001 tcggcat
//
LOCUS       DATLKL010000424         1517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_129186, whole genome shotgun sequence.
ACCESSION   DATLKL010000424 DATLKL010000000
VERSION     DATLKL010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129186"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VM713_05615"
     CDS             complement(<1..182)
                     /locus_tag="VM713_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HVH83778.1"
                     /translation="MRQIVLDTETTGLEPELGHRIIEIGCVELVNRRATGRTFHRYLN
                     PEREIDDAALAVHGISR"
     gene            complement(194..661)
                     /gene="rnhA"
                     /locus_tag="VM713_05620"
     CDS             complement(194..661)
                     /gene="rnhA"
                     /locus_tag="VM713_05620"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HVH83779.1"
                     /translation="MTAEQTTGVVEIYTDGACRGNPGPGGWAALLRRGTEEKEIHGAE
                     PHTTNNRMELTAVIRALESLRRPVEARVYTDSEYVRRGITEWLGSWKARGWRTADKKP
                     VKNQDLWQRLDELAAPHRIKWHWVPGHAGVEGNERVDRLANEAIDALARGGLR"
     gene            complement(680..1321)
                     /locus_tag="VM713_05625"
     CDS             complement(680..1321)
                     /locus_tag="VM713_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVH83780.1"
                     /translation="MEDVFGWELLQIGAWGSARELLARGRTRRQSLIAAPSFPLGADI
                     IARPAHLPVTSDSIDAALLPHTLEFAADPYSILREVDRILTGEGQLLILGFRPWSLWG
                     LRARASRSGFPPGMRRVLSGNLVREWLVLLGYEVVAATRYLYLSPWSRGLSPGKETGR
                     LLHPGLTYPLPGGAYLLKARKRVYTMTPIRPRLREQRPAVLGGLVKPTTRLGH"
BASE COUNT          258 a          514 c          499 g          246 t
ORIGIN      
        1 cgcgagatac cgtgcaccgc gagcgccgcg tcgtcgatct cgcgctcggg attcaagtaa
       61 cgatgaaagg tgcggccggt cgcgcggcgg ttcacgagct cgacacagcc gatctcgatg
      121 atccggtgtc cgagctcggg ctcgaggccc gtcgtctcgg tgtcgaggac gatctgtcgc
      181 atggggcgcg agtctaccgc aagccgcccc gcgctaacgc gtcgatggcc tcattggcga
      241 ggcgatcgac gcgctcgttc ccctcgacgc ccgcgtgtcc cggcacccag tgccatttga
      301 tgcggtgggg agccgcgagc tcatcgagac gctgccagag gtcctgattc ttgacgggct
      361 tcttgtccgc ggtgcgccag ccgcgcgcct tccacgagcc aagccattcg gtgataccgc
      421 gccggacata ttccgaatcg gtgtagacgc gcgcctcgac cggacggcgg agcgattcca
      481 gcgcgcggat cactgccgtg agctccatgc ggttgttggt ggtatggggc tcagcgccgt
      541 ggatctcctt ctcctcggtg ccgcgccgaa gaagcgcagc ccaaccgccc gggcccggat
      601 tgccgcgaca cgcgccgtcg gtgtagatct cgacgacgcc cgttgtttgc tcggctgtca
      661 ccttttgagg cgtgagtggc taatgcccca agcgcgtcgt cggcttcacg agtccgccca
      721 gcaccgcggg ccgctgctcg cgcagtcgcg ggcggatcgg cgtcatggtg tagacccgct
      781 tgcgcgcctt gaggaggtac gcgccccccg ggagggggta cgtgagcccg gggtgcagca
      841 accgccccgt ttccttaccg ggagagaggc cgcggctcca gggactcaag tacaggtagc
      901 gcgtggccgc aacgacttcg tagccgagga gtaccagcca ctcgcgcacc aggttccccg
      961 acagcacccg gcgcatcccg ggcggaaatc cgctgcggct cgcgcgcgcg cgcaggcccc
     1021 acaggctcca gggacgaaag ccgagaatga ggagctgccc ctcgcccgtc aggatccgat
     1081 ccacctcgcg caggatcgag taagggtcag cggcaaattc gagcgtgtgg gggaggagcg
     1141 ccgcatcgat cgagtcgctc gtgaccggca ggtgcgctgg acgggcaatg atgtccgccc
     1201 cgagcggaaa gctcggcgcc gcaatcaggc tctgacgacg cgtgcggccc cgcgccagga
     1261 gctctcgtgc gcttccccac gccccgatct gcaagagctc ccacccgaag acgtcctcca
     1321 tcgcttcgcc gacaagctcg gactcggcag caagcactga acgcccgagt gggctccccc
     1381 accaggcact taaggccgca tcccgttcag caatggttga agtgcgaact ggcacgatag
     1441 aaactctggt aaatatgtga acttacgggt taaatttcgg gccctgaacc agtagcgtgc
     1501 caataccaag gacccgc
//
LOCUS       DATLKL010000425         1649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_117919, whole genome shotgun sequence.
ACCESSION   DATLKL010000425 DATLKL010000000
VERSION     DATLKL010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117919"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..172)
                     /locus_tag="VM713_05630"
                     /pseudo
     CDS             complement(1..172)
                     /locus_tag="VM713_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928447.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
     gene            complement(334..1206)
                     /locus_tag="VM713_05635"
     CDS             complement(334..1206)
                     /locus_tag="VM713_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVH83781.1"
                     /translation="MAEFLQFTFSGITVGAIYALVGLGFSIIYNASQVINFAQGEFVM
                     IGGLTTAVLEADGTPLPLAAAVAILAAVLAGLALERLAVEQAKGASVATLIIITIGAS
                     IFLRGLAQVVFDRNYHSLRAFSGEHPINVAGATIVPQTLWVLVTTLLIVLVLEWFFGR
                     TLLGKAMLATSYNRLAAQLVGIDARAVLLLSFGLSAMLGAIGGILIAPISLTSYDVGV
                     MLGLKGFSAAVFGGMGNSKGAIAGGLILGILEALGAGYVSSAYKDAIAFVIILLVLFV
                     APRGLFGRGGTEQV"
     gene            complement(1325..>1649)
                     /locus_tag="VM713_05640"
     CDS             complement(1325..>1649)
                     /locus_tag="VM713_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVH83782.1"
                     /translation="LLVASQLPDGDPQKLPGMAYTKAYPERYHDDVSTFGGHAYDALM
                     LLVGAIERTGSTDPAMVRDALENTKNYIGLDGIYSMSATDHGGLDQASFKMVEVRNGD
                     WKFLY"
BASE COUNT          322 a          553 c          551 g          223 t
ORIGIN      
        1 cgagaagccc gacggcgacg gcacccccca gcatcgccag cgggggcggc cagccatagc
       61 gcgccgtcag cacggcggag gcataggcgc caaggccgaa aaatccagcg tggccgaggc
      121 tgatctgccc ggcatagcca atgagcagat tgagaccgac cgcgacgacc gcattaaggc
      181 caatcaggat cgctacattg aagaagaaat tgtttgggag cagccacggc agcgtcgcga
      241 tgacggcggc catcgcgaga agcccacggt agcgcgcaag gcccgccgtc gccgcgcgtc
      301 gccgcgcgcc cgggcgccgc ggtcccggtc ccctcatacc tgctccgtcc cgccccggcc
      361 gaacaggccc cggggagcca cgaacaggac gagaagaatg atcacgaagg caatcgcgtc
      421 cttgtaggca gacgagacgt agccggcgcc gagcgcctcg aggatgccga gaataagccc
      481 gcccgcgatt gcccccttgc tgttccccat gccgccaaag accgcggcgc tgaacccctt
      541 gagccccagc atgaccccca cgtcgtagga ggtcagcgag atcggggcaa tcaggatgcc
      601 gccgatcgca ccgagcattg ccgaaagacc gaaggagagc agcagcaccg cgcgcgcatc
      661 gatgccgacg agctgggcgg cgaggcggtt gtaggaagtc gccagcatcg ccttgccgag
      721 aagcgtgcgg ccgaagaacc actcgagcac cagcacgatg aggagggtgg tcaccagcac
      781 ccacagggtc tggggtacga tggtggcgcc ggcgacgttg atcggatgtt cgcccgagaa
      841 ggcgcgcagc gagtggtagt tgcggtcgaa caccacctgg gccaggccgc gcaggaagat
      901 ggaggcgccg atcgtgatga tgatgagggt ggcgaccgag gcgcccttcg cttgctcgac
      961 cgccagccgc tcaagcgcga gcccggcgag cacggcggcc agaatcgcga ccgcggcggc
     1021 gagcgggagc ggggtgccgt cagcctccag caccgcggtc gtgagccctc cgatcatgac
     1081 aaactcgccc tgggcgaagt tgatcacctg gctcgcgtta tagatgatcg agaagccgag
     1141 gccgacgagc gcgtagatcg cgcccacggt aatacccgag aaggtgaact gcagaaactc
     1201 ggccaagcct acgctgcctc cagccggccg cgcaccagac cgctctacgt ccgcttgcgc
     1261 ccgccgtacg gccccgcgat cccgctgaaa gccgccccgc cgcggctccg ccagcgcgag
     1321 gagcctagta gaggaacttc cagtcgccgt tgcgcacttc caccatcttg aaggaggcct
     1381 ggtcgaggcc gccatggtcc gtggccgaca tgctgtagat tccgtcgagt cctatgtagt
     1441 tcttggtatt ctcgagcgcg tcgcgcacca tcgccggatc ggtcgatccc gtgcgctcga
     1501 tggccccaac caatagcatg agcgcgtcgt acgcgtgccc gccgaaggtc gagacgtcgt
     1561 catggtagcg ctcggggtag gccttggtgt aggccatccc cggcagtttc tgcgggtcgc
     1621 cgtccggaag ctgactggca acaagcagc
//
LOCUS       DATLKL010000426         6126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_185630, whole genome shotgun sequence.
ACCESSION   DATLKL010000426 DATLKL010000000
VERSION     DATLKL010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185630"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..235)
                     /gene="ffs"
                     /locus_tag="VM713_05645"
     ncRNA           complement(138..235)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VM713_05645"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(251..342)
                     /locus_tag="VM713_05650"
     tRNA            complement(251..342)
                     /locus_tag="VM713_05650"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(306..308),aa:Ser,seq:gga)
     gene            complement(472..747)
                     /locus_tag="VM713_05655"
     CDS             complement(472..747)
                     /locus_tag="VM713_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83783.1"
                     /translation="MRSFLWLAPLLAMLSGCHPFRHFTYACHKPQGYMRATSIPPLKI
                     PPGLAPPDTTNALRLPALNEPAPPPRTGKQPCLDQPPPFKVVKPPQA"
     gene            complement(792..1673)
                     /gene="dapA"
                     /locus_tag="VM713_05660"
     CDS             complement(792..1673)
                     /gene="dapA"
                     /locus_tag="VM713_05660"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003317384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HVH83784.1"
                     /translation="MSFSGSLVAIVTPMRPDGALDLEAWVRLLDFHLANGTSAVVVGG
                     TSGESATLRELELRELTQRACEHVRGRLAVIVGAGTSSTATTVERVRTLSDLPIDGFL
                     LVTPAYNRPTQEGLYQHFAAAAAASAKPILLYNVPSRTAVDMKPATVARLTRVARIAG
                     VKEALPELSRVRELVDVVTKGFAVLSGDDATAREAIGCGARGVISVTANVAPRAMSQM
                     VAAALAGDATRARELDAPLASLHRDLFVEANPIPVKWALAQMGLIGAGLRLPLTPLSE
                     AHHATVRAAARAAGALA"
     gene            1838..2368
                     /locus_tag="VM713_05665"
     CDS             1838..2368
                     /locus_tag="VM713_05665"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HVH83785.1"
                     /translation="MKQHLAVSAIGSDRTGMVHELTRVISESGGNISESRMANLGTEF
                     AMLLLVSGNWHALAKLETELKRLAETSNLSIHLRRTEPRAPRTDMLPYSIDVVCLDQA
                     GIVSGLAGFFATRGIDIAEVSTRSYPAAHTGAPMFAVQMIVNVPSRIHVAHLREEFME
                     FCDSLNLDAILEPVKS"
     gene            2374..2841
                     /locus_tag="VM713_05670"
     CDS             2374..2841
                     /locus_tag="VM713_05670"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVH83786.1"
                     /translation="MPQIKTGSKVPEFSLPATGGGTWRLKDAAGGKLVIYFYPRDLTS
                     GCTREAQDFRDLYGAFRKAGVEIVGVSRDSVASHDKFAAKETLPFPLLADADERVCRV
                     FDVIKPKSLYGRKYLGIERSTFLVDAAGKLRQEWRKVKVPGHAEAVLEAARSL"
     gene            2879..4294
                     /locus_tag="VM713_05675"
     CDS             2879..4294
                     /locus_tag="VM713_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HVH83787.1"
                     /translation="MKGRSRGGRRLFVLDTNVLIHDPTAIFRFEEHDVYLPMVVLEEL
                     DAHKKGLSEASRNVRQVSRFLDDMMRDVTNEQIGQGLKLPSGYSGNHGKRPSTGRLFF
                     QTRQLRSALPESLPGQGGDNAILAQTLALQNEHPEARVILVSKDINLRIKAAVLGVHA
                     EDYYSDKTLEDSDILYSGLTALPGDFWEQHGKDMRSWKESDRTFYEITGPAVREWHPN
                     QGVFEDTPEGIEGIARKIRGDTATIELTRDYRNERHNVWGVTARNREQNFALNLLMDP
                     EIDFVTVLGPAGTGKTLLALAAGLMQTLETNRFVEIIMTRVTIPLGEDIGFLPGTEEE
                     KMEPWMGALMDNLEVLTGSQEGGNWGRAATNDLLRNRIKIRSLNFMRGRTFLNRFLIL
                     DEAQNLTPKQMKALITRAGPGTKLACLGNIAQIDTPYLTEMTSGLTYAVNRFRGWAHS
                     GHVTLVRGERSRLADYASDIL"
     gene            4456..5997
                     /locus_tag="VM713_05680"
     CDS             4456..5997
                     /locus_tag="VM713_05680"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HVH83788.1"
                     /translation="MRPVLIVLASWILASATVAFGEPPMPSTGFLNGPTAGSNLPDLG
                     SPAETILSKSDEFRLGAMVAKELRDQNVLIEDPEVSEYINDIGKRLASQSEMGGEYFH
                     YFVIRDNTINAFAVTGGYVFFYAGLVLATETESELAGVMAHETAHITQHHIARMLADQ
                     SRQSLTTAAAMIGAILLGALGGGGQAVEGGIVAAQGMAAQHQINFTRDNEWEADRVGI
                     SYLAGAGFDPYGMGEMFDTMARHSGLAQTYIPAMLIDHPVDSERIAEQRSRAAQFPSR
                     TGHDSQSYELIRERVRVLMATGDTDLAAQYAQKIAHGGDNMGNQYGRALALINGNRAN
                     GEEAVGILTRLVQQHEGLTELYSALGQAQAKAGHVSEALATFKHAETLFPRNVPVTVR
                     YAQTLMAAGRAGEAHTMLLDLFNLVPPTPDQIHLTALAASAAGDPGDAYFYMGEYQIA
                     NGDLPLAIQQIQLALAAPGTSTIQRQRYRARLDEVREFLASTRKPRLTDNSDQGPGHG
                     GGRAH"
BASE COUNT         1102 a         2123 c         1991 g          910 t
ORIGIN      
        1 ggtggatgcg cccggtgtcg agcgcattga cgagcgcgcg ccgcacgtgc tcctgcccga
       61 tgagctcgga gaagttcttc ggacgccatt tgcgcgcgag cgcggtgtag ctcattccat
      121 atccagttgg aggcggcccg caccagcttg gcctccggca cccgacatcg ccgctgccgc
      181 tgctgccttc cggccctgac gggattcacg actggtcgtc gcggagaaac cgatgcgggc
      241 caccacggtt tggcggagag ggtgggattc gaacccacga acaccggtga agatgttact
      301 ggaattccag tccagcgcct tcgaccgctc ggccacctct ccgatcaatg acttacagat
      361 gattcctcaa aagaactcga accaggttcg tcccctggaa aaagggcggc gcatgataat
      421 gcatggcgcc cggtgacgca tccggccgcg tgaccgcctc gtgagccgcg ctcaggcctg
      481 cgggggcttc accactttaa agggcggcgg ctggtcgagg cagggctgct tcccggtgcg
      541 cggcggcggc gccggctcgt tgagcgccgg cagacgcagc gcgttggtcg tatcaggcgg
      601 agcgagcccg ggcggaatct tcagcggcgg aatgctggtc gcccgcatgt agccctgcgg
      661 cttgtggcat gcataggtga aatgccggaa cgggtgacac ccgctcagca tagccaggag
      721 cggcgcgagc cacaggaatg atctcatctt tgtttccaac ccccagcgca aaggcccctg
      781 tttctgacgc ctcatgccag cgcgcctgcg gcgcgggcgg ccgcccgcac cgtggcgtga
      841 tgagcctcag agagcggcgt gagaggcaat cttaacccag cgccgatgag ccccatctgc
      901 gccaacgccc atttgaccgg aatcgggttc gcctcgacga acaggtcccg gtgcaggctc
      961 gcgagcgggg cgtcgagctc gcgcgctcgg gtggcatcgc cagcgagcgc cgcggccacc
     1021 atctgcgaca tcgcccgcgg ggcaacgttc gccgtcaccg aaatcacgcc gcgcgccccg
     1081 cagccgatcg cctcgcgcgc cgttgcgtca tcgccgctca gcaccgcgaa gcccttcgtg
     1141 accacatcga ccagctcgcg cacccggctc agctccggca gcgcctcctt cacgccggcg
     1201 atgcgcgcga cgcgcgtcag gcgcgccacc gtcgccggct tcatgtcgac agccgtgcgg
     1261 ctcggaacgt tgtagagcag gatcggcttg gccgaggcgg cggcggccgc ggcgaagtgc
     1321 tggtagagcc cctcctgcgt cgggcggttg taggcgggcg tgaccagcag aaatccgtcg
     1381 atgggcaggt ccgagagcgt cctgacgcgc tcgaccgtcg tcgcagtgct gctggtgccc
     1441 gcccccacga tgaccgcgag ccgcccgcgc acatgctcgc aggcccgctg cgtcagctcg
     1501 cgcagctcca actcgcgcag tgtcgccgac tcgccgctgg tgccgccaac cacgacagcg
     1561 ctggtgccgt tcgccaggtg gaaatcgagc agccgaaccc aggcttccag gtcgagcgcg
     1621 ccatcggggc gcatcggagt gacgatggca accaggctgc cggagaacga catacggctc
     1681 tcagacggac taaagggcga ggcacggatg ttacgagcgg ggctcccgcg gaggcaagcc
     1741 tcgagcgcgc gtggcgccgc gggatgggtc gcaaccggcg ggcgctcccg gtacactcgg
     1801 taaccgcagg ccggcaaaag cactccgcca ccgacagatg aagcagcacc tggccgtttc
     1861 ggcgatcggc agcgaccgaa cgggtatggt ccacgagctg acccgcgtca tcagcgagtc
     1921 cggcggcaat atcagtgaga gtcgcatggc gaatctcggc accgagttcg cgatgctgct
     1981 gctggtctcc ggcaactggc acgcgctcgc gaagctcgaa accgagctca agcgcctcgc
     2041 cgagaccagc aacctgagca ttcacctgcg gcgcaccgag ccgcgcgcgc cgcgcaccga
     2101 catgctgccg tactcgatcg atgtggtgtg cctcgatcag gcgggcatag tgtcggggct
     2161 cgccggcttc ttcgcgacgc gcggcatcga catcgccgag gtctcgaccc gcagctaccc
     2221 ggccgcgcac accggcgcgc cgatgttcgc cgtgcagatg atcgtcaacg tgccaagccg
     2281 cattcacgtc gcgcacctgc gcgaggagtt catggagttc tgcgactcgc tcaacctcga
     2341 cgccattctc gagccggtca agtcctgaaa gagatgccgc agatcaagac ggggagcaag
     2401 gtgccggagt tttccctgcc cgcgacgggc ggcggcacgt ggaggctgaa ggacgcagcg
     2461 ggcgggaagc tcgtcatcta cttctatccg cgcgacctga cttccggctg cacgcgcgag
     2521 gcgcaggact tccgcgacct ctacggcgcc ttccgcaagg cgggggtcga gatcgtcggc
     2581 gtatcgcgcg acagcgtcgc ctcacacgat aagttcgccg ccaaagagac gctgccgttc
     2641 ccactgctcg ccgatgccga cgagcgcgtc tgccgggtgt tcgacgtcat aaaaccgaaa
     2701 agcctgtacg gcagaaagta tctcggcatc gagcgcagca cgttcctcgt ggatgcggcg
     2761 gggaagctgc gacaggaatg gcgcaaggtg aaagtaccag gtcacgcgga ggcagtgctt
     2821 gaagcagcgc ggagtctatg aacccggtcc cccggcagcc tcgggcgagg cgcgcgcggt
     2881 gaaaggcagg tcacgcggcg gacggcggct gttcgtgctc gacacgaacg tgctgatcca
     2941 cgacccgacg gcgatcttcc gcttcgagga gcacgacgtt tacctgccga tggtggtgct
     3001 cgaggagctc gacgcgcaca agaagggtct gtcggaggcc tcgcgcaatg tgcgccaggt
     3061 gagccgcttc ctcgacgaca tgatgcgcga cgtcaccaac gagcagatcg gccagggact
     3121 caagctcccg agcggctact cgggcaacca cggcaagcgc ccgtccaccg ggcgcctgtt
     3181 cttccagacg cgtcagctgc gcagcgccct gcccgagagc ctgcccggcc agggcggcga
     3241 caacgcgatc ctcgcgcaga cgctcgcgct gcagaacgag catccggaag cgcgggtgat
     3301 cctggtgtca aaggacatca acctgcgcat caaggctgcg gtcctcggcg tgcacgccga
     3361 ggactactac agtgacaaga cgctcgagga ctcggacatc ctctacagcg gcctgacggc
     3421 gctgcccggg gacttctggg agcagcatgg caaagacatg cgctcctgga aggaaagcga
     3481 ccgcaccttc tatgagatca ccgggcccgc ggtgcgcgag tggcatccta accagggggt
     3541 gttcgaggac acccccgaag gcatcgaggg catcgcgcgc aagatcaggg gcgataccgc
     3601 caccatcgag ctcacccgcg attaccgcaa cgagcgccac aatgtctggg gcgtcaccgc
     3661 gcgcaaccgc gagcagaact tcgcgttgaa cctgctcatg gacccggaga tcgatttcgt
     3721 gacggtgctc ggaccggctg gcaccggcaa gacgctgctg gcgctcgccg ccggtctcat
     3781 gcagaccctc gagaccaacc gcttcgtcga gatcatcatg acgcgcgtca ccatccccct
     3841 tggtgaagac atcggcttcc tgccgggaac cgaggaagag aagatggagc cgtggatggg
     3901 cgcgctcatg gacaacctcg aagtgctcac cggctcgcag gaaggcggca actggggccg
     3961 cgcggcgacc aacgacctgc tgcgcaaccg catcaagatc cgctccctga acttcatgcg
     4021 cggtcgcacc ttcctcaacc gcttcctgat cctcgacgag gcgcagaacc tgacgcccaa
     4081 acagatgaag gcgctgatca cccgtgccgg ccccggcacg aagctcgcct gcctcggcaa
     4141 catcgcgcag atcgacacgc cctatctcac cgagatgacc tccgggctca cttacgcagt
     4201 gaaccgcttc cgcggctggg cccattccgg gcacgtgacg ctggtgcgcg gcgagcgctc
     4261 gcgcctcgcc gactacgcct ccgacattct ctaggcgcag cgcatactta cattgtcaaa
     4321 gtcggccgcg ggtccggatt ggtgtacgct tggacgcgcg gcagcgaagc ggtcagggat
     4381 gtgcccgcgg agcctttgta agcgtctgta agcgcgagga aactcgcccg tgccgaccac
     4441 ggtcaaatga accttatgcg tcctgtgctg atcgtgctcg cgagctggat cctcgcctcg
     4501 gcaaccgtcg ccttcggcga gccgccgatg ccgagcaccg gcttcctgaa cggaccgacg
     4561 gcgggctcga acctcccgga tctcggcagc cccgccgaga cgatattgtc gaagagcgat
     4621 gagttccgcc tcggcgccat ggtcgcgaag gagctgcgcg accagaacgt cctgatcgag
     4681 gacccggaag tcagcgagta catcaacgac atcggcaagc ggctcgcctc gcagagcgag
     4741 atgggcggtg agtacttcca ctacttcgtg atcagggaca acacgatcaa cgccttcgcc
     4801 gtgacgggcg gctatgtctt cttctacgcc ggcctggtgc tcgccaccga gaccgaatcg
     4861 gagctcgcgg gcgtgatggc ccacgagacc gcgcacatca cccagcacca catcgcgcgc
     4921 atgctcgccg accagtcccg gcagagcctg acgaccgcgg ccgccatgat cggggccatc
     4981 ctgctcggtg cgctcggcgg cggcggccag gcggtcgagg gcggcatcgt cgccgcccaa
     5041 ggcatggcgg cgcagcatca gatcaacttc acgcgcgaca acgaatggga agccgaccgc
     5101 gtcggcatca gctacctcgc gggcgcgggc ttcgatccct acggcatggg tgagatgttc
     5161 gacaccatgg cgcgccactc ggggctcgcc cagacctaca tcccggcgat gctgatcgat
     5221 cacccggtgg actcggagcg aatcgccgag cagcgttccc gggcggcaca gtttccgtcg
     5281 cgcaccgggc acgactcgca aagctacgag ctcatccgcg agcgggtgcg cgtgctgatg
     5341 gcaacgggcg acaccgacct ggcggcgcag tacgcccaga agatcgcgca cggcggcgac
     5401 aacatgggca accagtacgg ccgggcgctg gcgctcatca acggcaaccg cgccaacggc
     5461 gaggaggcgg tcggaatcct cacgaggctc gtgcagcagc acgagggcct caccgagctc
     5521 tattcagccc tcggccaggc gcaggccaag gccggacacg tcagcgaggc gctcgcgacc
     5581 ttcaagcacg ccgaaacgct ctttccgcgc aacgtgccgg tgacggtccg ttacgcgcag
     5641 acgctgatgg ccgcaggccg cgcgggtgag gcgcacacca tgctgctcga tctcttcaac
     5701 ctcgtgccgc ccacccccga tcagatccac ctcacggcgc tcgcggcgag cgccgccggc
     5761 gaccccggcg atgcctactt ctacatgggc gagtaccaga tcgccaacgg cgatctgccg
     5821 ctcgccatcc agcagatcca actggcgctc gcggctcccg gcaccagcac catccagcgc
     5881 cagcgctacc gtgcgcgcct cgacgaggtg cgcgagttcc tcgcgagcac acgcaagccc
     5941 cgcctcaccg acaacagcga tcagggaccc gggcacggcg gcgggcgtgc ccactgacgg
     6001 cgccggccgc ctccgccccc gccgcctccg tctttagtgc gatagagcgc cggcgcgctg
     6061 ctgctagaat gcccgccggt cttcgctcta ccggtgtcat gcaagccacg atgcgcattc
     6121 ccctgg
//
LOCUS       DATLKL010000427         1560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_129237, whole genome shotgun sequence.
ACCESSION   DATLKL010000427 DATLKL010000000
VERSION     DATLKL010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129237"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VM713_05685"
     CDS             <1..1188
                     /locus_tag="VM713_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVH83789.1"
                     /translation="MVFLAQNRFLKVLLLGAISLLADGCAKHAPPAAPKPAVTVVRLE
                     AKPVSLTTELPGRISAFRIAEVRPQVNGVVLKRLFKEGDLVAAGQQLYQIDPAPYEAT
                     LASARASLAHARASAVAAGLTAERYAALVEAHAISRQDYDNAVATLQQDQADVASGEA
                     AVRYAEINLTYTKVYSPISGRTGRSSVTEGALVTANQATSLVTITQLDPVYVDLTQPS
                     TTLVRLKRELAAGQIRRADGDEAPAELLLEDGSSYDSAGKLEFSEVTVDQGTGSVTLR
                     AIFPNPQGLLLPGMFVRATIEEGVREGAIVAPQQGITHSPDGGATALVVGANDTVEKR
                     TVELNRALGDQWLVTRGLAAGDRLVVAGVQAIKPGMQVVVQGQTAPDKRTADRSALAL
                     AAR"
     gene            1219..>1560
                     /locus_tag="VM713_05690"
     CDS             1219..>1560
                     /locus_tag="VM713_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH83790.1"
                     /translation="MARFFIDRPIFAWVIAILVMMAGVLALLSLPVAQFPALAPPAVA
                     ITVTYPGASAETVEGTVVQPIEQQMTGLDGLLYMSSESDKDGSITITVSFAQGTNPDI
                     AQVQVQNKLQLA"
BASE COUNT          278 a          528 c          492 g          262 t
ORIGIN      
        1 atggtcttcc tagcccagaa ccgcttcctc aaggtcctgc tgctcggtgc aatcagcctg
       61 ctcgcggacg gttgcgcaaa gcacgcgccg ccagcggcac cgaagccggc cgtcacggtt
      121 gtacggctcg aagcgaagcc cgtgtcgctg acgacggagc tgccgggccg catatccgcc
      181 ttccgaatcg ccgaggtccg cccacaggtg aacggtgtcg tgctcaagcg actgttcaag
      241 gaaggcgatc tggttgccgc cggccagcag ctctaccaga tcgaccccgc gccctacgaa
      301 gcgacgctcg ccagtgcgcg agcatcgctg gcgcatgcgc gcgcttccgc ggtcgcggca
      361 ggccttaccg ccgagcgcta tgccgcactt gtcgaagcgc acgcgatcag ccggcaggat
      421 tacgacaacg ccgttgcaac gctgcagcag gaccaggccg acgtggcatc aggcgaggcc
      481 gcggtgcgtt acgcggaaat caacctcacc tacaccaagg tctactcccc aatcagcggg
      541 cgcaccggcc gctcctcggt caccgagggc gctctcgtca cggcaaacca ggccacgagc
      601 ctggtcacga tcacacagct cgacccggtg tacgtggatc tcacgcagcc gagcacgacg
      661 ctcgtgcgct tgaagcgcga gcttgccgcc gggcagatcc ggcgggccga cggcgacgag
      721 gcaccggcgg agctccttct ggaggacggg agctcttacg actctgctgg aaaactcgaa
      781 ttctctgagg tcacggtcga tcagggcacg ggctcagtca ccctgcgcgc gattttcccc
      841 aatcctcaag gcctgctgct gccgggtatg ttcgtgcgcg caaccatcga ggagggcgtt
      901 cgcgagggcg ccatcgtcgc tccgcagcag ggcattactc actctcccga tggcggtgcc
      961 acggcgctcg tcgtgggtgc gaacgacacc gtcgagaaac gcaccgtgga gctcaatcgt
     1021 gcgctcggtg atcagtggtt ggtcacacgc ggactggcgg ctggcgatcg tctcgtcgta
     1081 gccggtgttc aagcaatcaa gcccggtatg caggttgtcg tgcaaggaca gaccgcgccg
     1141 gacaaaagga cggccgaccg atccgcgttg gcgctcgccg cgcgctaatc ggcaatccac
     1201 tatccgaggg agttcaccat ggctcgtttc ttcatcgatc gaccgatctt cgcctgggtt
     1261 atcgccatcc ttgtgatgat ggcgggcgtg ctcgcgctgc tcagcctgcc cgttgcgcaa
     1321 tttccggctt tggccccgcc cgcggtggcg atcacggtca cctaccccgg cgcgtccgcg
     1381 gagacggttg aaggcaccgt cgttcagccc atcgagcagc agatgaccgg gctcgacggg
     1441 ttgctctaca tgtcctccga gagcgacaaa gacggcagca tcacgatcac cgtgagcttc
     1501 gcgcagggca cgaatcccga catcgcgcag gtgcaggtgc agaacaagct gcagctcgcc
//
LOCUS       DATLKL010000428         1901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_38924, whole genome shotgun sequence.
ACCESSION   DATLKL010000428 DATLKL010000000
VERSION     DATLKL010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38924"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VM713_05695"
     CDS             <1..194
                     /locus_tag="VM713_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83791.1"
                     /translation="AGFDVLTTEPPKEGNPLLALHAPNFILTPHVAWASDEAMQFLAD
                     QLIDNIEAFVRGQPQNLVT"
     gene            211..1197
                     /locus_tag="VM713_05700"
     CDS             211..1197
                     /locus_tag="VM713_05700"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HVH83792.1"
                     /translation="MRIAIVGAGAVGGYVGVKLALAGERVTFLARGANLEAIRSRGVK
                     LIMDDGAELVARDVQATADYSSAGPQDVVILAVKAHQVDAVAPEVPKLLGPETCVVTM
                     QNGIPYWYFHRHGGPFEGRCIAAVDRAGACLRGIPPSRVIGCVVYPASVLESPGVVRH
                     IEGDRFPVGELDGSASPRVRAVSQSLTAAGFQAPVLEDIRAEVWLKLWGNLTFNPISA
                     LSRATLVEICQFPATRQLAASMMAEAQAVAHKLGISFRVPIERRIAGAERVGRHKTSM
                     LQDLEAGRAMETDALLGSVLELARMTEVPTPTLAAVYALLKLLGRPLEVSAS"
     gene            1204..>1901
                     /locus_tag="VM713_05705"
     CDS             1204..>1901
                     /locus_tag="VM713_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HVH83793.1"
                     /translation="MQDTAIFVESMALGGDGLRVGVKDSIDIAGFPTRMGSACLGDAL
                     PAAQHAAVVRALLDAGCRIVGKTNLHELAFGLTGINRWTGTPVNPRAPGRVPGGSSSG
                     SAVAVAAGLADFALGNDTGGSIRLPAACCGLVGLKPSYGRVSRSGVHPARSTLDCVGP
                     LAPTVSMIERAMEMIDSTFRAQGGPRCPAVGWVEVEASPAMTAAARAALEHAGLALRP
                     VALPSFAAAFSAALV"
BASE COUNT          271 a          656 c          665 g          309 t
ORIGIN      
        1 gcgcgggatt cgatgtgctg accacggagc cgcccaagga aggcaatccg ctgctcgcgc
       61 tgcacgcgcc gaacttcatt ctcacgcccc acgtggcgtg ggcgagcgac gaggcgatgc
      121 agttcctcgc cgatcagctc atcgacaaca tcgaggcgtt cgtgcgcggg cagccgcaga
      181 acctcgtcac ctgacggagc caagcgcacc atgagaatcg cgatcgtggg ggcaggggcc
      241 gtcggcggct acgtgggggt caagctggcg ctcgccggcg aacgcgtcac gtttctcgcc
      301 cgcggcgcca acctggaggc gatccgcagc cgcggcgtca agctcatcat ggacgacggc
      361 gccgagctcg tggcgcgcga tgtacaggcg accgcggact actcatccgc cggaccccag
      421 gacgtggtga ttctcgccgt caaggcccat caggtggatg cggtcgcgcc tgaggtgccg
      481 aagctcctcg gtcccgagac ctgcgtcgtc acgatgcaga acggaattcc ctactggtac
      541 tttcaccgcc atggcggtcc gttcgaaggc cggtgtatcg ccgcggtcga tcgcgcgggc
      601 gcatgcctgc gtgggattcc gccgagccgc gtcatcggct gcgtcgtcta ccccgcctcg
      661 gtgctggagt ctcccggagt cgtccgtcac atcgaggggg atcgtttccc ggtgggcgag
      721 ctcgatgggt cggcgagccc gcgggtgcgg gcggtgtctc aatccctgac cgcggccggg
      781 tttcaggcgc cggtgctcga ggacatccgc gcggaagtct ggctcaagct ctggggcaac
      841 ctcacgttca atccgatcag cgccttgagc cgcgcgacac tcgtggaaat ctgccagttc
      901 cccgccaccc gacaactggc ggcgtcgatg atggcggaag cacaggccgt ggcgcacaag
      961 ctcggcatca gctttcgtgt cccgatcgaa cggcgcatcg ccggtgcgga gcgggtgggc
     1021 aggcacaaga catccatgct gcaggatctc gaagcgggcc gcgccatgga gaccgatgcg
     1081 ctgctcggct cggttctcga gctggcgcgc atgacggaag tgcccacgcc gacactcgcg
     1141 gccgtgtatg cgctgctcaa gctcctcggc aggccgctcg aggtgagtgc ctcctgagcg
     1201 tcgatgcagg acaccgccat cttcgtggag agcatggccc tgggtggcga cggcctccgt
     1261 gtcggcgtca aggactcgat cgacatcgcc ggttttccca cccgcatggg gagcgcctgc
     1321 ctcggtgacg cgctgccagc ggcgcagcat gccgccgtcg tgcgcgcgct gctcgatgcg
     1381 ggctgccgga tcgtcggcaa gaccaacctg cacgagctcg ccttcggcct caccgggatc
     1441 aatcgctgga ccggtacccc cgtcaacccg cgcgcccccg ggcgcgtgcc gggtgggtcc
     1501 tcgagcggat ccgccgtggc cgtggcggcg ggactcgcgg actttgcgct cggcaacgac
     1561 accggcggct cgatccgcct gcctgcggcg tgttgcggcc tcgtcggcct caagccttcc
     1621 tatggaagag tcagccgcag cggggtccac ccggcgcgct cgaccctcga ttgcgtgggg
     1681 ccgctggcgc cgaccgtgtc gatgatcgaa cgcgccatgg agatgatcga ttcgacgttc
     1741 cgcgcgcagg gcgggccgcg gtgtcccgcg gtcggttggg tcgaggtgga ggcaagcccc
     1801 gcgatgaccg ccgcggcacg cgccgcgctc gagcacgccg gcctcgcgct gcgtccggtc
     1861 gcgctaccgt cctttgcggc ggccttctcg gcggcactgg t
//
LOCUS       DATLKL010000429         5300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_27637, whole genome shotgun sequence.
ACCESSION   DATLKL010000429 DATLKL010000000
VERSION     DATLKL010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27637"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VM713_05710"
     CDS             complement(<1..265)
                     /locus_tag="VM713_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83794.1"
                     /translation="MSLPRSVVALVVFLSGLCAVPPRAAAEPLAVLLKPLEWRSIGPF
                     RGGRVLSVTGVPGEPSHFYFGAVNGGVWETRDAGRGDDVPLRVG"
     gene            335..1510
                     /locus_tag="VM713_05715"
     CDS             335..1510
                     /locus_tag="VM713_05715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HVH83795.1"
                     /translation="MKRGEERRLAAGHPWVFSNEIDTDASPLGAFAPGALAELRTHRG
                     GFVGYGYVNPHALICARLLSRDAAHPVDRALIEARLKRALALRTRLGAAPFYRWVFGE
                     SDALPGLVLDRYGDVVVGQIATAGMEALKDEVAAAVRAVLAPAGLFWKNDTAARDLEQ
                     LPQEAQLAFGAVPDELTVEEAGLAFRAPLAHGQKTGWFYDQTANRARLARYIAPGARV
                     LDVCSYAGAWAIAALRAGAARACCIDSSHSALEFAAANAARSGRVVETLKADAFEALR
                     KLNDQGARYDVVILDPPAFIKRKKDIPQGQAAYRKLNQLALGVLDDEGMLVSCSCSYH
                     LAPEELMSAIQSAARHSGRFVQILEAGGQSPDHPVHPAIPETRYLKAFFCRVTRELG"
     gene            1532..2335
                     /gene="lgt"
                     /locus_tag="VM713_05720"
     CDS             1532..2335
                     /gene="lgt"
                     /locus_tag="VM713_05720"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HVH83796.1"
                     /translation="MVKFPGFDPIAFHLGPVKVHWYGIMYLLGFGCAWWLARLRAAAP
                     RSTWKPADVDDLIFFGMLGVILGGRIGYVLFYGLKFWAGDVWYPLKIWEGGMSFHGGL
                     IGVAIAMMLFAWRRGRNVGDVYDFTAPLPPLGLFFGRIGNFINGELWGKTTTVPWGFE
                     VNGQVRHPSQLYEAALEGLLLFAVLWWFTARPRPRLAPSALFLLIYGAVRSLIEFVRV
                     PDEHIGYLAGGWLTEGQLLSLPMIIVGVVLLAWAYHTRTPSGNFAAAPP"
     gene            2332..3126
                     /gene="thyA"
                     /locus_tag="VM713_05725"
     CDS             2332..3126
                     /gene="thyA"
                     /locus_tag="VM713_05725"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HVH83797.1"
                     /translation="MKQYLELLRRIRTEGVRKGDRTGTGTLSLFGHQLRFDLAQGLPL
                     VTTKKIHLPAVIYELLWFLRGDTNIAWLREHGVTIWDEWADERGELGPVYGRQWRAWG
                     AADGRTIDQITQAVERLKRDPDSRRNLVSAWNVGELERMALAPCHLLFQLYVAAGRLS
                     LQVYQRSCDFFLGVPFNIASYALLTHMLAQQCDLEPGELVWTGGDCHLYLNHLEQADL
                     QLSRTPYPLPRLVLRRRPPSMFDYEYDDFEFVDYRSHAAIRAPVAV"
     gene            3123..3626
                     /locus_tag="VM713_05730"
     CDS             3123..3626
                     /locus_tag="VM713_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HVH83798.1"
                     /translation="MSGTGRTAEPLIEVRASPLHGLGVFALRRIPRGSRVIEYLGERI
                     SHEEADRRYEHKSADDNHTFLFIADDTTVIDAGVDGNEARFVNHACDPNCESVIEHAR
                     VFLDAIRTIAPGEELTYDYQIQREADDPPDIDVVFACRCGKARCRGTMMWPPRRARGA
                     TAVGKRR"
     gene            3632..>5300
                     /locus_tag="VM713_05735"
     CDS             3632..>5300
                     /locus_tag="VM713_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HVH83799.1"
                     /translation="MNHPRPFRLAAVVAATGIVYGDIGTSPLYTMDAALGAAGNFDAE
                     AVLGVLSLIFWALTISVTLKYLTVIMRADNQGEGGILALFALAQRRLITGSTWARVAV
                     GLALAGTAFFFCDALITPAISVLGAVEGLEVLNPGLKSWVIPATIVVLAVLFAYQHRG
                     TAAVAALFGPIMVVWFVTIAVIGAIPIARNPHVLVALNPACGLTLLFHRPQAALAMIG
                     AAFLAITGGEALYADMGHFGKRPVRIAWFALVGPALVLNYFGQGALLLELGKPVAHPL
                     FHLVPEPVLPWLVILATAAAVIASLATISGAFSMASQAVHLDLLPRLRVLQTSALEQG
                     QIYVPIVNWLVFPAVILFVVGFGSSDALGGAYGAAVVGTMAVTTVLGASVAATQWNWP
                     KWLVAGIFGLIMVTDLAFVAGNMTKIPQGGWIPLTLGAILYLIFMTWRSGRLELRAAL
                     AKLAVPRSELPKLVAGVQRVPGTGVFLASNPTLVPSALIRNIEHNAVVHQRVIILNVE
                     IADTPRQDPAGRLNIQEPVPGVYYISARFGFMETPDVAEALKACRTRG"
BASE COUNT          760 a         1831 c         1791 g          918 t
ORIGIN      
        1 cccccacccg taggggaacg tcatcgcctc gccccgcatc acgcgtctcc cacacgccgc
       61 cgttcactgc gccgaagtag aagtgactcg gctcgcccgg gacgcccgtg acgctgagca
      121 cgcgcccgcc gcggaacggc ccgatggagc gccactccag cggcttgagc agtaccgcga
      181 gcggctcggc cgccgcgcgc ggcggcaccg cgcacaggcc ggacagaaac acgacgagcg
      241 ccacgacgct gcgtggtaag gacacgtttg cacctcggag aaaggagggc aattgtatac
      301 ttgccgcccc ttgcgcaacg gatccttact gatattgaag cgcggcgagg agcgacgcct
      361 cgccgccgga catccctggg tgttcagcaa cgaaatcgac accgatgcga gtccgctcgg
      421 cgcctttgcg ccgggcgcgc tcgccgagct ccgcacccac cgtggcggtt tcgtcggcta
      481 cggctacgtc aatccgcacg ccctcatctg cgcgcggctg ctgtcgcgtg atgccgcgca
      541 ccccgtcgac cgcgcgctca tcgaggcgcg cctgaagcgc gcgctcgcgc tgcggacgcg
      601 gctcggcgcg gctccttttt accgctgggt attcggcgag tccgacgcac tgccgggcct
      661 ggtgctcgat cgctatggcg acgtcgtcgt cggccagatc gccaccgccg gcatggaggc
      721 gctcaaggac gaggtggcgg ccgcggtgcg cgcggtgctc gcccccgccg ggctcttctg
      781 gaagaacgac accgcggcgc gcgatctcga gcaactgccg caggaggcgc agctggcctt
      841 cggcgcggtc ccggacgagc tcaccgtgga ggaggcgggg ctcgccttca gggccccgct
      901 cgcgcacgga cagaagaccg gctggttcta tgaccagacg gcgaatcgcg cccgcctggc
      961 ccgctacatc gcgccgggtg cgcgcgtgct cgacgtctgc tcttacgccg gtgcgtgggc
     1021 gatcgcggcg ctgcgtgcgg gagctgcgcg cgcctgctgc atcgactcct cgcattcggc
     1081 gctcgaattc gcggcggcca acgccgcgcg cagcgggcgc gtggtcgaga cgctcaaggc
     1141 cgatgccttc gaggcgctgc gcaagctgaa cgatcagggc gcgcgctacg acgtggtgat
     1201 cctcgatccg ccggcgttca tcaagcgcaa gaaggacatt ccgcaaggac aggccgccta
     1261 ccgcaagctc aatcagctcg ccctcggagt gctcgacgac gaaggcatgc tggtgtcgtg
     1321 ctcctgctcc tatcatctcg ctcccgagga gctgatgagc gccatccaga gcgccgcgcg
     1381 gcatagcgga cgcttcgtgc agatcctcga ggccggcggc cagtcgcccg accacccggt
     1441 gcatccggcg atccctgaga ctcgttatct caaagcgttc ttctgccgcg tgacgcgcga
     1501 gctcggctaa accgctaaac taacgcgcct catggtgaag ttccccggct tcgatccgat
     1561 cgcgttccac ctcggcccgg tcaaggtgca ctggtacggg atcatgtacc tgctcggctt
     1621 tggctgcgcc tggtggctgg cgcggctccg cgccgcggcg ccgcgttcca cctggaagcc
     1681 ggccgacgtc gacgatctga tcttcttcgg catgctcggc gtgatcctcg gcgggcgtat
     1741 cggctacgtg ctgttctacg ggctgaagtt ctgggcgggc gatgtgtggt acccgctcaa
     1801 gatctgggag ggcggcatgt ccttccacgg cggcctgatc ggtgtggcca tcgcgatgat
     1861 gttgtttgca tggcggcgcg gtcgcaacgt gggcgacgtc tacgatttca ccgcgccgct
     1921 gccgccgctc ggactgttct tcgggcggat cggcaacttc atcaacggcg agctatgggg
     1981 caagacgacc acagtgccgt ggggcttcga ggtgaacggc caggtacgcc acccgtcgca
     2041 gctctacgag gcggctctcg aggggttgct gctgtttgcc gtactgtggt ggttcaccgc
     2101 gcggccgcgg ccgcggctcg cgccgtcggc cctgttcctc ctcatttacg gcgccgtgcg
     2161 ctcactcatc gagttcgtac gcgtgccgga cgagcacatc ggctatctgg ccggcggctg
     2221 gctcaccgag ggccagttgc tgtcgctgcc gatgatcatc gttggtgtgg tgctgctggc
     2281 gtgggcctac cacacgcgca cgccgtcggg caatttcgcc gcggcgccgc catgaagcag
     2341 tatctcgagc tgctgcgccg gatccgcacc gagggcgtgc gcaagggcga tcgcaccggc
     2401 accggcacgc tgtcgctgtt cggccatcag ctgcgcttcg acctggcgca gggcttgccg
     2461 ctggtgacca ccaagaagat tcatctgccg gcggtgattt acgagctgct gtggtttctg
     2521 cgtggcgata ccaacatcgc ctggctccgc gagcacggcg tgaccatctg ggacgaatgg
     2581 gcggacgagc gtggtgagct cggtccggtg tacggccggc aatggcgcgc ctggggcgca
     2641 gccgacggcc gcaccatcga tcagatcacc caggccgtcg agcgcctcaa gcgcgacccc
     2701 gactcgcgcc ggaacctggt gagcgcctgg aacgtgggcg agctcgagcg gatggcgctc
     2761 gcgccctgcc atctgctgtt ccagctgtac gtcgctgccg gccggctgtc gctgcaggtg
     2821 tatcagcgca gctgcgattt cttcctcggc gtgcccttca acatcgcaag ctacgcgctc
     2881 ctcacccata tgctcgcgca gcaatgcgat ctcgagccgg gagagctcgt ctggacgggc
     2941 ggcgattgcc acctgtacct gaatcatctc gagcaggcgg acctgcagct gtcgcgcacg
     3001 ccgtacccgc tgccgcgcct cgtgctgcgg cgccgtccgc cgagcatgtt cgactatgag
     3061 tacgacgact tcgagttcgt ggattatcgc agtcacgcag ccatccgcgc gccggtggcg
     3121 gtatgagcgg gacggggcgc acggcagagc cgctcatcga ggtgcgggcc tcaccgctgc
     3181 acggactcgg agtgtttgcg ctacggcgca tcccgcgcgg ttcgcgcgtg atcgagtacc
     3241 tcggcgagcg catctcgcac gaggaggccg accggcgcta cgagcacaag agcgcggacg
     3301 acaaccatac gttcctcttc atcgccgacg acaccacggt gatcgacgcc ggcgtcgacg
     3361 gcaacgaggc gcgctttgtg aatcatgcct gcgatccgaa ctgcgagtcg gtgatcgagc
     3421 acgcgcgcgt ctttctcgac gcgatccgca ccatcgcgcc tggggaggag ctcacctacg
     3481 actaccagat ccagcgcgag gccgatgatc cgccggatat cgacgtcgtc ttcgcctgcc
     3541 gctgcggtaa ggcccgctgc cgcggcacca tgatgtggcc gccgcggcgg gcgcgcggcg
     3601 ccaccgccgt gggcaagcgg cgctgaccgg catgaaccac ccacgccctt tcaggctggc
     3661 ggccgtggtc gccgcgaccg gcatcgtgta cggcgacatc ggcacgagtc cgctctatac
     3721 aatggacgcg gcgctcggcg ccgcgggcaa cttcgacgcc gaggcggtgc tcggcgtgct
     3781 gtcgctgatc ttctgggcgc tcacgatctc ggtgaccctc aagtacctca cggtgatcat
     3841 gcgggccgac aaccaggggg agggcgggat cctcgcactg ttcgcgctgg cccagcggcg
     3901 tctcatcacc ggcagcacct gggccagggt ggcggtggga ctggcgctcg cggggaccgc
     3961 cttcttcttc tgcgacgcgc tcatcacacc ggcgatctcg gtgctgggcg cggtcgaggg
     4021 cctcgaggtg ctgaacccgg ggctcaagag ctgggtgatt ccggcgacga tcgtggtgct
     4081 cgcggtgttg ttcgcctatc agcaccgcgg gactgcggcg gtggcggcgc tgttcggccc
     4141 catcatggtc gtgtggttcg tgacgatcgc ggtgatcggc gcgattccga tcgcgcgtaa
     4201 cccgcacgtg ctggtggcgc tcaatcccgc ttgcggactg actctgctct ttcaccggcc
     4261 gcaggcggcg ctggcgatga tcggcgcggc gttcctcgcc atcaccggcg gcgaggcgct
     4321 ctacgccgac atgggccact tcggcaagcg gccggtgcgc atcgcgtggt ttgctctggt
     4381 cggcccggcg ctggtgctca actacttcgg ccagggggca ctgctcctcg agctcggcaa
     4441 gccggtcgcg catccgctct ttcacctggt gccggaaccg gtgctgccgt ggctggtgat
     4501 cctggcgacg gcggcagccg tgatcgcctc gctggcgacg atctccggcg cattctccat
     4561 ggcgagccag gcggtgcacc tcgatctgct gccgcggctg cgggtgctgc agacctcggc
     4621 gctcgagcag ggtcagatct acgtaccgat cgtcaactgg ctggtgttcc ccgcggtgat
     4681 tctcttcgtc gtcggcttcg gctcttccga cgcgctcggc ggcgcctatg gcgccgcggt
     4741 ggtcggcacc atggcggtca ccaccgtcct cggcgcctcc gttgccgcca cgcagtggaa
     4801 ctggccgaag tggctggtag cagggatctt cggactgatc atggtgaccg acctcgcctt
     4861 cgtagcaggc aacatgacta agatccccca gggcggctgg attccgctca ccctcggcgc
     4921 catcctgtat ctgatcttca tgacctggcg cagcggccgc ctggagctgc gtgccgcgct
     4981 tgccaagctc gccgtgccac gcagtgagct gccgaagctc gtggccggcg tgcagcgcgt
     5041 gccgggcacc ggcgtgttcc tcgccagcaa cccgacgctg gtgccgtcgg cgctgatccg
     5101 caatatcgag cacaacgccg tcgtgcacca gcgcgtcatc atcctcaacg tcgagatcgc
     5161 cgacacgccg cgccaggacc cggccggaag gctcaacatc caggagcccg tgccgggcgt
     5221 gtactacatc agcgcgcgct tcggcttcat ggagacccca gacgtggccg aggcgctcaa
     5281 ggcctgccgc acgcgcgggc
//
LOCUS       DATLKL010000430         2202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_38981, whole genome shotgun sequence.
ACCESSION   DATLKL010000430 DATLKL010000000
VERSION     DATLKL010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38981"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1443)
                     /locus_tag="VM713_05740"
     CDS             complement(196..1443)
                     /locus_tag="VM713_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVH83800.1"
                     /translation="MSWLTPLFVLAVLVGLGLELWLAQRQAAAVARHRGTVPQPFAAS
                     ISAQEHTKAADYTIAKVRFGRISLGVDALLTLLLTVGGGLAALDALWGRVHLREPWLG
                     LAVIASAALVIRLVGMPLSLWRTFALEARFGFNRTTVALYLSDLAKSVALAVVLGGPL
                     VLATLALMERAGRWWWLAAWGLWLGVMLLMAWAWPAFIAPLFNRFTALADVALKARIE
                     ALLERCGFASKGVFVVDSSRRTSHGNAYFTGIGRHKRIVFFDTLLKTLGDGEVEAVLA
                     HELGHFRLRHVRQRLLVSVAAAFAGLAVLGWLTTQSGFYHALGVPNPSPHAALLLFVL
                     VVPAFTFFLTPLASLWSRRHEFQADAFATRHASAAELAAALVKLHRDNASTLTPDPLY
                     AAFYYSHPPPLERITKLQEQAQA"
     gene            complement(1454..>2202)
                     /locus_tag="VM713_05745"
     CDS             complement(1454..>2202)
                     /locus_tag="VM713_05745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH83801.1"
                     /translation="FGLAAASLAAALLYFWLDRREAPRGTLALPPPPERFDWRHLLTF
                     RPEYWVLVAVCVSFYSVIFPFRSTFAIKYLQEAQGYTLAQAGRLNSYVFLAAVFATPV
                     FGLILDRIGRNTLLLAAGALLLPPSFLVLGALPGGAGLSTALLGISFSFVPAVLWPTV
                     VRYAPPEHLGTAYGLMTTLQNAGLFGANIIAGYLNDLNGAGAANPGGYLPMLWFFGLL
                     SLTAFALTLVLRALDHGAAPRTAAVAGSRD"
BASE COUNT          413 a          741 c          769 g          279 t
ORIGIN      
        1 ccagcacgcc attgccgctg tgaaggtgaa cgcgccgtct ctgagccact gccgtgcgtg
       61 gccggcgtac tccggccaga ggtgcgccag ccccgtcgcc agcgcataga accccaccat
      121 ccatacggct gcgccgatca ccacgccgag cacgctgcgc gtcatgttac taacccctcg
      181 gtcgcccgaa ggtcttcatg cttgagcctg ttcctgcagc ttggtgatgc gctcgagcgg
      241 cggcggatgt gagtagtaga acgccgcata tagcggatcc ggggtcagag tgctggcgtt
      301 gtcgcgatgc agcttcacga gcgccgcggc gagctcggcc gcgctcgcat gccgggttgc
      361 aaaggcatcc gcctggaact cgtgccgccg cgaccacagc gaggcgagcg gggtgaggaa
      421 gaaggtgaag gccggcacca ccagcacgaa cagcagcagc gccgcatgtg gcgaggggtt
      481 cggcacgccg agtgcgtggt agaaaccact ctgtgtagtg agccagccga gcacggcgag
      541 tccggcaaag gccgccgcca ccgacacgag cagccgttgt cgtacgtgtc tgaggcggaa
      601 gtggccgagc tcgtgcgcca gcaccgcctc cacctcgccg tcaccgaggg tcttaagcag
      661 tgtgtcgaag aatacgatgc gcttatggcg gccgatgccg gtgaagtagg cattgccgtg
      721 gctggtgcgc cgcgagctgt cgacgacgaa gacgcccttg gaagcaaagc cgcagcgctc
      781 gagcagcgcc tcgattcgcg ccttgagcgc tacgtccgcg agcgccgtga agcgattgaa
      841 gagcggtgcg atgaacgccg gccacgccca ggccatgagc agcatcacgc cgagccacag
      901 cccccacgcc gccagccacc accagcggcc ggcgcgttcc atcagcgcaa gagtggcgag
      961 gaccagcggc ccgccgagca ccacggcgag ggccacgctc ttggcgaggt cgctcaagta
     1021 cagcgcgacg gtcgtgcggt tgaacccgaa gcgcgcctcc agcgcaaaag tgcgccacag
     1081 cgacagcggc atgcccacca ggcgaatcac gagcgcggcg ctcgcgatca ccgccagtcc
     1141 gagccagggc tcgcgcagat gcacgcgacc ccacagcgca tcgagcgcgg cgaggccgcc
     1201 gccgaccgtg agcagcagcg tgagcagagc gtccacaccc aggctgatgc gcccgaagcg
     1261 caccttggcg atggtgtagt cggccgcctt ggtgtgctcc tgtgccgaga tgctggccgc
     1321 gaagggctgc ggcaccgtgc cgcgatgacg tgcgacggcg gcggcctggc gctgggccag
     1381 ccacagctct aggcctaagc ccacgagcac cgcgagcacg aacagcgggg tgagccagct
     1441 catgcctctg gcgtcagtcg cggctgccgg ccactgcagc ggtccgcggc gcggcaccgt
     1501 ggtcgagcgc ccgcagcacc agcgtcagcg cgaacgcggt gaggctcagc aggccgaaga
     1561 accacagcat gggcagatag ccgcccgggt tggctgcgcc agcaccgttc aggtcgttga
     1621 gatagccggc gatgatgttc gcgccgaaga gcccggcgtt ctgcaacgtg gtcatgagac
     1681 cgtaggcggt gccgaggtgc tcaggcggcg cgtagcgcac gaccgttggc cacagcacgg
     1741 cgggcacgaa cgagaagctg atgccgagca aggcggtcga cagcccggcc cctcccggca
     1801 gcgctcccag caccaggaag ctcggcggca gcagcagcgc acccgcggcc agcaacagcg
     1861 tgttgcgccc gatgcggtcg aggatcaagc cgaacaccgg ggtggcaaac acggcggcga
     1921 gaaacacgta gctgttgagc ctgccggctt gcgccagcgt gtaaccctgc gcctcctgca
     1981 ggtacttgat ggcgaaggtg ctgcggaagg ggaagatgac cgaatagaag ctcacgcaca
     2041 ccgcgaccag cacccagtac tcgggcctga aggtcagcag gtggcgccag tcgaagcgct
     2101 ccggcggcgg cggcagggcg agcgtcccac gtggcgcctc gcgccggtcg agccagaagt
     2161 aaagcagtgc tgccgccaag gaggcggccg ctagaccgaa cg
//
LOCUS       DATLKL010000431         2969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_38997, whole genome shotgun sequence.
ACCESSION   DATLKL010000431 DATLKL010000000
VERSION     DATLKL010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38997"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VM713_05750"
     CDS             <1..911
                     /locus_tag="VM713_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid dehydrogenase"
                     /protein_id="HVH83802.1"
                     /translation="AAADALRLSRGMSYKNAMAGLPFGGGKAVIIADARTAKTPELFA
                     AFGRLVDSLRGRYVTAEDVGTTIADMAHVARVTRYVSGLGRSPGGAGGDPAPKTALGV
                     FLGMQAAVRFRLGRADFAGLKVAVQGAGGVGYHLCGLLSAAGAHLAVADVQPQAVERV
                     CDEFKATPVAPVHMLEEEADVLAPCALGAILTARSIARLRVPVVAGAANNQLALGEDG
                     EALRAAGILYAPDYVINAGGIISVAREYYGGTEAQVFEDLQGIPARLTEIFERARREG
                     RTTNEIADQMARERLGSGREAPRLVA"
     gene            1036..1797
                     /locus_tag="VM713_05755"
                     /pseudo
     CDS             1036..1797
                     /locus_tag="VM713_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
     gene            2006..2902
                     /locus_tag="VM713_05760"
     CDS             2006..2902
                     /locus_tag="VM713_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HVH83803.1"
                     /translation="MRKPTLGRGLADLLGTAVARPTVTPGPGEELARLPLDLLQRGRY
                     QPRTDMRTDTLTELADSIKAQGVVQPIVVRALEPGAGGAPGSPQRYEIIAGERRWRAA
                     QMAGLSEIPAVIRRIPDSAAIAVALIENIQRENLNPLEEARALTRLISEFGLTHQEAA
                     DSVGRSRAGVSNLLRLLDLPAEICALLEQRSLEMGHARALLALGGRRQQVEVGLLVAK
                     KGLSVRETEALVRRLTAPTVHGADGDGEPARDPNVERLEQELAEKLGARVAIQHSSKG
                     RGKLVVSFNSLDELDGILTHIQ"
BASE COUNT          462 a         1060 c         1041 g          406 t
ORIGIN      
        1 aggctgcagc ggacgcgctg cgcctctcgc gcggcatgag ctacaagaac gccatggcgg
       61 ggctcccgtt tggcggcggc aaggcggtca tcatcgccga tgcgcgcacg gccaagactc
      121 cggagctctt cgccgccttc ggccggctgg tcgattcgct gcgcgggcgc tacgtgacgg
      181 ctgaggatgt cggcacgacc atcgctgaca tggcccatgt ggcccgcgtt acccgctacg
      241 tatcgggcct cggccgctcg cccggtgggg ccggcggcga cccggctccg aagaccgcgc
      301 tcggcgtgtt cctcggcatg caggcggcgg tgagattccg gctcggccgc gccgacttcg
      361 ctggtctcaa ggtcgcagtc cagggcgcag ggggcgtcgg ctaccacctg tgcggtctgc
      421 tctcggccgc gggagcgcac ctcgcggtgg ccgacgtgca gccgcaggcc gtcgagcgcg
      481 tgtgcgacga gttcaaggcg acgccggtcg ctcccgtgca catgctggag gaggaggcgg
      541 acgtgctcgc tccctgcgcg ctcggcgcga ttctcaccgc tcgctcgatc gcgcggttgc
      601 gcgtgccggt ggtggcgggc gcggcgaaca accagctggc gctgggtgag gacggcgagg
      661 cgctgcgcgc ggcgggcatc ctctacgcgc cggactacgt catcaatgcc ggcggcatca
      721 tcagcgtggc gcgtgagtac tacggcggca ccgaggccca ggtcttcgag gatctgcagg
      781 gaatcccggc gcggctcacc gagatcttcg agcgcgcccg gcgcgagggc cgcaccacca
      841 acgagatcgc cgaccagatg gcgcgcgagc gcttaggcag cggtcgcgag gcgccgcgcc
      901 tcgtagcctg aagcgcgagg taagccgcgg cgggcaggcc cgtttcgggg gctcccgccc
      961 ctgtcgccac gcgtagacac caaagcccgg gggttacggc agcacgcggg cggcggtgaa
     1021 gtacactgtg cgcccatgaa gcgggtcatt gccatcgcga accagaaggg cggcgtcggc
     1081 aagaccacca ccgcggtcaa tctcgccgct tcattggccg ccacccgccg gcgcgtgctg
     1141 ctcatcgacc tcgacccgca aggcaacggc accatgggca gcggcatcga caagtcccag
     1201 gtctcccgca gcgcctgcga ggtgctgctc ggggaggtgg acattgcttc ggcgctcgtg
     1261 ccggtccccg acggcgggta catgctacta ccggccaatc aggacctcac ccacgcggag
     1321 gtgcggctgc tgacgctccc catcgggcgc gagaccaagc tccgccacgc gctgcactcg
     1381 cagcgggacg ccttcgacct catcttcatc gactgcccgc cggcgctcaa catgctcacc
     1441 gtgaatgcgc tggtcgccgc cgacagcgtt cttatcccca tgcagtgcga gtattacgcg
     1501 ctcgaggggc tcacggcgct gctggcgacc gtcgagcaga tccgcgatgc ggtcaacccg
     1561 ggcctcggcc tcgagggcgt gctgcgcacc atgttcgacc cgcgcaataa tctcgccaac
     1621 gaggtgagca gccagctgat cggccacttc ggggacaagg tgtttcgcac ggtcatcccg
     1681 cgcaacatcc gtctcgcgga ggcgccctcc ttcggcaagc cggcgctgtt ccacgacaag
     1741 gaatcgcgcg gcgcactggc gtatctcgcg ctcgcagggg agatgatccg gcgtgaggaa
     1801 gaagcgaact ccgaagcgcg tccggccacg cgcccgaacg cggccggcaa cggcggcagc
     1861 gcgcacgcgg agccggccgc cgcgcccgag cgcgagtccg gcgccgaagc cgacaacgaa
     1921 tccgacaccg aatccgatgc cgcagccgac gccgcggtcg gcggcggaaa aggcgcatga
     1981 gctcaacgtc acgggaaaca cccgcatgag aaagccgacg ctcgggaggg ggctcgcgga
     2041 tctgctcggc acggcggtcg cccgtccgac ggtcacgccc ggccccgggg aggagctcgc
     2101 caggctcccg ctcgatctgc tgcagcgcgg ccgctatcag ccgcgtaccg acatgcgcac
     2161 cgacacgctc accgagctcg ccgactcgat caaggcccag ggcgtggtgc agcccatcgt
     2221 ggtgcgggcg ctcgagcccg gggccggcgg cgcgcccggc tcgccccagc gctacgagat
     2281 catcgccggc gagcgccgct ggcgggcggc gcagatggcc gggctcagcg agatccccgc
     2341 cgtcatccgc cgcattccgg attcggccgc gatcgccgtc gcgctcatcg agaacatcca
     2401 gcgcgagaac ttgaatccgc tcgaggaggc gcgcgccctc acccgcctca tcagcgaatt
     2461 cgggctgacg caccaggagg cggccgactc cgtcgggcgc tcgcgcgccg gcgtgagcaa
     2521 tctgctgcgg ctgctcgatc tcccggccga gatctgcgcg ctcctcgagc agcgctcgct
     2581 cgagatgggc cacgcacgcg cgctgctcgc actcggcggc cgccgccagc aggtcgaagt
     2641 ggggctgctg gtggccaaga aggggctctc ggtgcgcgag accgaggccc tggtgcgccg
     2701 tctcacagcg ccgaccgtgc acggcgccga cggcgatggc gagccggccc gcgacccgaa
     2761 cgtcgagcgg ctcgagcagg agctggcgga gaagctcgga gcccgtgttg caatccagca
     2821 ttccagcaag ggccgcggca agctggtcgt cagcttcaac agcctcgatg agctcgacgg
     2881 gatcctcacg cacatccaat agcgaggggc ggatacaata ggcagtagcg ctgtggactg
     2941 ccgtccacgc gctctctata atcccgcgg
//
LOCUS       DATLKL010000432         1826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_61518, whole genome shotgun sequence.
ACCESSION   DATLKL010000432 DATLKL010000000
VERSION     DATLKL010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61518"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VM713_05765"
     CDS             complement(<1..542)
                     /locus_tag="VM713_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83804.1"
                     /translation="MNRPLVLTAFLALVLAACSRHEAPPSPAAPAAEAPAAVNAPAAA
                     PAPAAATGAAGPAQSEIEQARGAQESADGGVNEKPESSDASLERMVALPPDAQLLAGR
                     WKPGVNYDPLVPGQPTRVEPGKVEVLEIMWLGCPHCYALEPFIRAWLKTKPAYIEFVR
                     VPVIWGPPQRAHAKLFYTLKA"
     gene            complement(547..1251)
                     /locus_tag="VM713_05770"
     CDS             complement(547..1251)
                     /locus_tag="VM713_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVH83805.1"
                     /translation="MRTMPNTLRVGWMGLAMSALALAQPLAVAQNSPAAAPGAAPSVP
                     FTTGKAASGEAKAALCMACHGPNGNSTNPEWPRLAGQSAVYIAEQLKLFRSGVRDNPV
                     MKPLAASLSDQDINDLAVYYEAQTPTGLEADPSYWKSGEALYLSGDAVRGVPACVACH
                     GPVGRGNLAAGYPALRAQQSVYVVKQLNDYAKGTRYAGPNPAQASRNGVMMLDLAKRL
                     TPEEIRDVASYVQGMR"
     gene            1343..>1826
                     /gene="yihA"
                     /locus_tag="VM713_05775"
     CDS             1343..>1826
                     /gene="yihA"
                     /locus_tag="VM713_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HVH83806.1"
                     /translation="MSRFQQARFLTSAAAPAQFPPDLGAEVAFAGRSNAGKSSAINAI
                     TQRRGLARTSKTPGRTRLLNFFEVAPQRRLVDLPGYGYAGGPQAERRAWQPLIDALHA
                     RASIKGLFLIVDARRGVTAGDEGLLEWVSPQQRVHVLLSKADKLTRAEAAGALRTAAD
                     E"
BASE COUNT          262 a          635 c          643 g          286 t
ORIGIN      
        1 gccttcagcg tgtagaagag cttcgcgtgc gcgcgctgcg gcggacccca gatgaccggc
       61 acgcgcacga actcaatgta tgcgggcttg gttttcagcc aggcgcgaat gaagggctcg
      121 agcgcgtagc agtgcgggca gccgagccac atgatctcga gcacctcgac cttcccaggc
      181 tcgacgcgcg tcggctgtcc cggcaccagt gggtcgtagt tgacgccggg cttccagcgg
      241 cccgccagca gctgcgcatc gggcggcagc gccaccatcc gctcgagcga agcgtcgctc
      301 gactcgggct tctcgttgac gcccccgtca gcggattcct gcgcgcctcg tgcctgctcg
      361 atctcgctct gggcgggccc ggcagcgccg gtggcagcag cgggtgccgg cgctgcggcg
      421 ggcgcattca ccgccgctgg cgcctcggct gcgggtgcgg cgggcgaagg tggcgcttcg
      481 tggcgcgagc aggccgcgag aacgagggcc aggaacgctg tcaggacgag cggccggttc
      541 atcggctcag cgcatgccct gcacgtagga ggcgacgtcg cggatttcct ccggagtcag
      601 gcgcttggcg aggtcgagca tcatcacgcc gttgcggctc gcctgggccg ggttcgggcc
      661 cgcgtaacgg gtgcccttgg cgtagtcatt gagctgcttc acgacgtaca ccgactgctg
      721 cgcgcgcagt gccgggtacc cggccgcgag attcccgcgg cccaccgggc cgtggcacgc
      781 gacgcaggcc ggaacccccc gcaccgcatc gccgctgaga tagagcgctt cgccggattt
      841 ccagtaggag gggtccgcct cgagcccggt cggggtctgc gcctcgtagt acaccgcgag
      901 gtcattgatg tcctggtcgc tcaaggaggc ggcgagcggc ttcatgaccg ggttgtcgcg
      961 cacgcccgag cggaagagtt tgagctgctc ggcgatgtag accgcgctct ggcccgcgag
     1021 acgcggccat tcggggttgg tgctgttgcc gttcggaccg tggcaggcca tgcacagcgc
     1081 cgccttggcc tcgccgcttg ccgccttgcc ggtcgtaaac ggtaccgagg gggccgcgcc
     1141 cggcgcagcg gccggggagt tttgtgcgac ggcgagcggt tgcgcgagcg ccagagcgct
     1201 catcgcaagt cccatccaac cgaccctgag ggtgttcggc attgttctca ttccggctta
     1261 ggtcccgcga agcgacggcg gcggattgta cactacggcc ccactcgggc cttcggcctc
     1321 ccgcgagcag caaaccctga aaatgagccg ctttcagcag gcgaggtttc tcacgagcgc
     1381 cgcggcgccg gcgcagtttc cgcccgatct cggcgccgag gtcgccttcg ccggacgctc
     1441 caatgccggc aagtcgagcg ccatcaacgc gatcacgcag cggcggggac tcgcgcgcac
     1501 cagcaagacg cccggccgta cccggctgct caatttcttc gaggtcgcac cgcagcggcg
     1561 cctcgtggac ctgccgggct acggctatgc gggcggaccg caagccgagc gccgcgcctg
     1621 gcagccgctc atcgacgccc ttcacgcgcg cgcgagcatc aaaggacttt tcctgatcgt
     1681 cgatgcgcgg cgcggcgtca ccgcaggaga cgaggggctc ctcgaatggg tctcaccgca
     1741 acagcgtgtg cacgtgcttc tctccaaggc cgacaagctg acccgcgccg aggctgccgg
     1801 cgcgctgcgc accgccgcgg atgagc
//
LOCUS       DATLKL010000433         1942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_219341, whole genome shotgun sequence.
ACCESSION   DATLKL010000433 DATLKL010000000
VERSION     DATLKL010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219341"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VM713_05780"
     CDS             complement(<1..559)
                     /locus_tag="VM713_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HVH83807.1"
                     /translation="MRRRPASARPGSTRVPAATGAPAERLQKLLARAGLASRREVEAW
                     IRAGRLTVNGAVATLGTRAHGADQVRLDGRPVRVQAAHSPLVLLCHRSPGEELATTLA
                     RLPRRAGKRFIAVSPMPRVDGGLELVTADGALGESLQRRVHALTSEFGVRVRGELGEP
                     QLRGVLGGELDSGIQLTVARCEAAGG"
     gene            complement(559..1266)
                     /gene="scpB"
                     /locus_tag="VM713_05785"
     CDS             complement(559..1266)
                     /gene="scpB"
                     /locus_tag="VM713_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HVH83808.1"
                     /translation="MSDSRIRNVVEAALLAAGQPLPVSELARLFDEGARPSTRKLEAA
                     LEALAAEYAGRGIELKHTASGYRIQVRRELAAEISRLFPERTVRYSRALLETLALIAY
                     RQPITRAEIEAVRGVAVNPNIVRTMIERNWVRVVGHRDVPGHPELLGTTREFLDYFGL
                     KSLDELPPLAELKAVGDINLQLALAGEELPGAAADSGAAGGADPADGEDAGEDDELSA
                     GGSGSHELVAAPYEPEG"
     gene            complement(1263..>1942)
                     /locus_tag="VM713_05790"
     CDS             complement(1263..>1942)
                     /locus_tag="VM713_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HVH83809.1"
                     /translation="IPLAEITRQYMQYIELMQELQLELAGEYLVMAATLAEIKSRMLL
                     PRPPSGLEEGEDPRAELVRRLQEYERYKRAAQDLDGLTRLERDVWPTVVELKERRVTR
                     TLPQITLQEMLVAFKEVVARAEMFAHHHIARERLSVGERMSDILAMLGSAGFVEFTRL
                     FRPEEGRMGVTVTFVAILELMREGLIDIVQAEAYAPLHVRAAPAGRTLHLVADNTAGA
                     VTGEEPS"
BASE COUNT          254 a          718 c          692 g          278 t
ORIGIN      
        1 ggccgccggc cgcctcgcag cgcgcgactg tgagctgtat gccgctgtcg agctcgccgc
       61 ccaggacacc ccgcagctgc ggctccccga gctcgccccg cacccgcacg ccaaactcgc
      121 tggtgagcgc gtgcacccgg cgctgcaggc tctcgcccag cgcgccgtcg gcggtgacca
      181 gctcgagtcc gccatcgacg cgcggcatgg gactcacggc gatgaagcgc ttgccggcgc
      241 gccgcggcag ccgcgcgagc gtcgtcgcca gctcctctcc cggcgagcgg tgacagagca
      301 gcaccagggg tgaatgcgcc gcttggacgc gcaccggccg cccgtcgagg cgtacctggt
      361 cggcgccgtg cgcccgggtc ccgagcgtcg ccaccgcgcc gttcaccgtg aggcggccgg
      421 cgcggatcca ggcttcgacc tcgcgacgcg aggccagtcc ggcgcgcgcc agcagcttct
      481 gcagtcgctc ggcgggagcg cccgtggcgg ccggcacgcg cgtgctgccc ggccgcgccg
      541 acgccgggcg ccggcgcatc agccctccgg ttcgtagggc gccgcgacca actcgtggga
      601 gccgctaccg ccggcagaga gctcgtcatc ttccccggcg tcctcaccgt ccgcggggtc
      661 ggcgccgccg gcagcgccgc tgtcggctgc agcgcccggc agctcctcgc cggcgagcgc
      721 cagctgcagg ttgatgtcgc cgacggcctt gagctcagcg agtggcggca gctcgtcgag
      781 gctcttgagg ccgaagtagt cgaggaactc tcgcgtcgtg ccgagcagct ccggatgtcc
      841 gggtacgtcg cgatgcccga ccacccgcac ccagttgcgc tcgatcatgg tgcggacgat
      901 gttgggattg accgcgacgc cgcgcaccgc ctcgatctcc gcgcgggtga tgggctggcg
      961 gtaggcgatc agcgcgagcg tctcgagcag cgcccgcgag tagcgcacgg tgcgctccgg
     1021 gaacagccgc gagatttccg cggccagctc gcggcgcacc tggatgcggt agccgctggc
     1081 ggtgtgcttg agctcgatgc cgcggccggc gtactcggcg gcgagtgcct cgagcgcggc
     1141 ctcgagcttc cgcgtgctcg ggcgcgcgcc ctcatcgaac agacgggcga gctcgctcac
     1201 gggcagcggc tggccggcag cgaggagcgc cgcttcgacg acgttgcgga tacgggagtc
     1261 gctcatgagg gctcctcgcc cgtcaccgcg ccggcggtgt tgtcggcgac caggtgcagg
     1321 gtgcgtcccg cgggcgcggc gcgcacgtgc agcggcgcgt aggcctcggc ctgcacgatg
     1381 tcgatgagcc cctcgcgcat cagctcgagg atcgccacga acgtgacggt gacgcccatc
     1441 cggccctcct caggacggaa cagccgcgtg aactcgacga agccggcact gcccagcatg
     1501 gcgagaatgt cgctcatgcg ttcgcccacc gacagccgct cgcgggcgat gtggtgatgc
     1561 gcgaacatct cggcgcgcgc caccacctcc ttgaaggcaa ccagcatctc ctgcagcgtg
     1621 atctgcggga gcgtgcgcgt cacgcgccgc tccttgagct cgacgaccgt cggccatacg
     1681 tcgcgctcga gccgcgtgag cccgtcgaga tcctgggcgg cgcgcttgta gcgctcgtat
     1741 tcctgcaggc gccgcaccag ctcggcacgc ggatcctcgc cctcctccag cccgctcggc
     1801 ggccgcggca ggagcatgcg cgatttgatc tcggcgagcg tcgccgccat caccaggtac
     1861 tcgccggcga gctcgagctg cagctcctgc atcagctcga tgtattgcat gtactggcgg
     1921 gtgatctcgg cgagcgggat at
//
LOCUS       DATLKL010000434         2342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_27682, whole genome shotgun sequence.
ACCESSION   DATLKL010000434 DATLKL010000000
VERSION     DATLKL010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27682"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VM713_05795"
     CDS             complement(<1..353)
                     /locus_tag="VM713_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HVH83810.1"
                     /translation="MEYRDYYQILGLQRTATADQVKTAYRRLARKYHPDVSKEANAEE
                     RFKEVQEAYEVLKDPEKRAAYDQLGSEWKTGQQFRPPPDWGSGFEFGGGRGGARTGSA
                     RGGDGEAYAEERFSD"
     gene            complement(675..1451)
                     /locus_tag="VM713_05800"
     CDS             complement(675..1451)
                     /locus_tag="VM713_05800"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HVH83811.1"
                     /translation="MRTIVTFQTGRAALAALAVALLTLAACSGEQQDWRAAEAAGTTE
                     AYKHFLEQHPDSELAGKARERVGDFAEDRDWQQASQLGTADAYRGFLAAHPAGHWAQE
                     ARIRIESFALGSIPRMQPQTPGLTASRPAGVKLLRLASASPAVPASAPAPAAAGATPA
                     VAADPAAARPAFSASAGKGYAVQLGAFGTAGSANREWHRLKLRFGPQLAGLSPQVVRG
                     DSPSGQLYRLQARASGEAQARAICDSLRAQSQPCVPVLPH"
     gene            1532..1903
                     /locus_tag="VM713_05805"
     CDS             1532..1903
                     /locus_tag="VM713_05805"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HVH83812.1"
                     /translation="MLDQLGDAPTFVAPGSRVAGDLETLGPLVVCGAVRGDGRVGGAL
                     SMSANAEWTGEVHARTAVIAGRVTGRLVVEDTLEVGPTAVIQADVVARTVAIARGAVI
                     EGAVTVTSGQPVIRFDERRIA"
     gene            complement(1916..>2342)
                     /locus_tag="VM713_05810"
     CDS             complement(1916..>2342)
                     /locus_tag="VM713_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83813.1"
                     /translation="PTETLVALETAMPGYQGFFVRKWRRSLRKKQAAWPDSYDFAGVR
                     GGLRAMTAELVRRHSEFATLEDYLNGYSITGARLASLAVPAHILTSLDDPMIPVGGLA
                     RLARPAALTVTITRYGGHCGFIESLAGLTWAERFVLERL"
BASE COUNT          369 a          795 c          808 g          370 t
ORIGIN      
        1 aaatcgctga acctctcctc ggcgtaagcc tccccgtcgc cgccccgggc gctgccagtg
       61 cgcgcgccac cgcgcccacc accgaactcg aatccgctgc cccaatccgg cggtggccgg
      121 aactgctgac cggtcttcca ctcgctgccg agctggtcgt aggcggcgcg cttctccggg
      181 tccttcagga cctcgtacgc ctcctgcact tccttgaagc gctcctcggc attggcctcc
      241 ttgctgacgt ccgggtggta cttgcgcgcg agtcgccggt acgccgtctt cacctgatcg
      301 gcggtcgcgg tccgctgcag gccgagaatc tgatagtagt cccggtattc catgaagctt
      361 ctattgagcc gaatgcccgc ggtgtcaacg ttggattcta tccgcgaaaa aaaaggcgcc
      421 gcgagcgggg gaagtcgcat cgcggcgcgc aggagcgaac acggggaagt tgttcgcaac
      481 tcaggtcacg gacgcgctca gggggcgcgt ccgacgaacc tcgcggtgat catccgattc
      541 accgcacccc gatcaggagc aaaggctgtg ccaaagagct aaaccatcta aaaacaatag
      601 cttgaagcac agggtcgggt ccccgggtga caggaattgt cactttggag cgaagcgcag
      661 cagagtcgta ccgttcagtg tgggagcacg ggcacgcagg gctgggactg cgccctgagc
      721 gagtcgcaga tcgcacgcgc ctgcgcctcg ccgcttgccc gggcctgcag ccgatagagc
      781 tgacccgagg ggctgtcgcc acgaacaacc tgaggcgaga ggcccgcgag ctgcggcccg
      841 aagcgcagct tgagacggtg ccattcgcgg ttggcgctgc ctgctgttcc gaaggcgccg
      901 agctgaaccg cgtagccctt gccggctgaa gcgctgaacg cgggccttgc tgccgcagga
      961 tctgccgcaa cggccggcgt ggcgcccgca gcggctggag ccggtgcact tgcgggaacc
     1021 gcgggcgaag cgctcgcgag gcgcagaagc ttcaccccgg cggggcgcga ggcggttagc
     1081 cccggcgtct gcggctgcat cctgggaatc gacccgagcg cgaagctctc gatgcggatc
     1141 cgcgcctcct gcgcccagtg tccggctggg tgtgccgcga gaaatccccg gtaggcatcg
     1201 gccgtcccga gctggctcgc ctgttgccag tcgcgatcct cggcgaagtc gcccacgcgc
     1261 tcgcgcgcct tcccggcgag ctcgctgtcg gggtgctgct cgaggaagtg cttgtacgcc
     1321 tcggtggtgc cggcggcctc ggcggcccgc cagtcctgct gctcgccgct gcatgctgca
     1381 agtgtgagca gcgccacggc tagcgcggcc agcgcggccc tgcctgtttg gaatgtgacg
     1441 atggtccgca ttttggcccc ctggcggtcc gtattatgga cccgcagcct ccagggagca
     1501 ccgcatgggt gaggacacac aacgtcgcag aatgctagac cagctcggcg atgcgccgac
     1561 tttcgtcgcg cccgggagcc gtgtggccgg cgacctcgag actctgggtc ccctggtggt
     1621 gtgcggcgcc gtgcgcggcg acgggcgcgt cggcggcgcg ctcagcatgt cggccaacgc
     1681 cgagtggacc ggcgaggtgc acgcgcgcac ggcggtgatc gccggccgcg tgaccgggag
     1741 gctggtcgtc gaggacaccc tcgaggtcgg cccgaccgcg gtgatccagg cggacgtcgt
     1801 cgcccgcacg gtcgcgatcg cgcgcggggc cgttatcgag ggcgccgtga ccgtgaccag
     1861 cggccaacca gtcatccgct tcgacgaaag acgcatcgct taagaaacgc agcgcttaaa
     1921 gccgttcgag tacgaaccgc tccgcccagg tgaggcccgc gagcgactcg atgaagccgc
     1981 agtgaccccc gtagcgcgtg atcgtcacgg tgagcgcggc gggacgcgcc aggcgcgcga
     2041 gtccccccac cggaatcatc ggatcatcga gcgacgtgag gatgtgcgcg ggcaccgcga
     2101 gtgaggcgag ccgcgcgccg gtaatcgagt agccgttgag gtagtcctcg agtgttgcga
     2161 attctgagtg ccggcgcacc agctccgccg tcatcgcccg caggccgccg cgcacgcccg
     2221 cgaaatcata gctgtcgggc caggccgcct gtttcttgcg cagcgagcgc cgccacttgc
     2281 gcacgaagaa tccctgatat cccggcatgg ccgtctcgag cgcgaccagg gtctcggttg
     2341 gc
//
LOCUS       DATLKL010000435         3957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_84118, whole genome shotgun sequence.
ACCESSION   DATLKL010000435 DATLKL010000000
VERSION     DATLKL010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84118"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VM713_05815"
     CDS             <1..610
                     /locus_tag="VM713_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVH83814.1"
                     /translation="EQALEWLRAHQCALIVLDVMLPGIDGFGVCRALREQGNNTPVLF
                     LTARGDPVDRVRGLEAGGDDYLAKPFHLEEFLLRVRAILRRWDWYRSASATVATAVLR
                     FGGNEVDFRAFRARAWNGEPQELTEKEAMILKVLAEHAGEIVSREDLLERVWGYDVFP
                     STRTVDNFILRLRKRFERDPASPRHFLTVWGVGYRFLVEGEP"
     gene            607..1578
                     /gene="hemC"
                     /locus_tag="VM713_05820"
     CDS             607..1578
                     /gene="hemC"
                     /locus_tag="VM713_05820"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HVH83815.1"
                     /translation="MSRPSLRIGTRASALALWQARHVEALLRAQRGAPPIELVHIRTS
                     GDAQREVPLWQTEGRAFFTREIDAALSAGTIDVAVHSLKDLPTTLAPGLALGAALARE
                     DPRDALLTRAGVSLRQLPPRPRIGTSSLRRRAFLARLRPDAVPTELRGNVPTRIERLE
                     RGDYDAIILAAAGLARLGLSQHVSEYLSPEEFPPAVSQGVIGVCARADDESTLALLRA
                     LDDAGARLAVSAERALLQRLEGGCQVPLGALATTRGAALNLQAAVCALDGSRELRARG
                     TASAAHGAVLTRADAVALGERIADELLGAGAAELIAHERAALAVTAP"
     gene            1575..2345
                     /locus_tag="VM713_05825"
     CDS             1575..2345
                     /locus_tag="VM713_05825"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HVH83816.1"
                     /translation="MSSASDTRPVVVTRAESADGPLSRELRSLGLEVLSWPAVSVTAS
                     DSSALEDAISRLESFAWIVFASRHAVAAVLERRPKPPAQPRVAAVGRATALTLSQRGW
                     PVHLTPPEANAAALMAAFAAQWRPSDAGARVLYPASSRALPTIAAGLRQLGAVVTQVE
                     AYRTEAAALDVAECRAWIERGGLGAVTFASPSAVTELARALGEENFARLLDAAPPVAI
                     GPTTARELAARGRNVTLADTATLHGLAVTTYRVMQQRA"
     gene            2360..3337
                     /gene="hemB"
                     /locus_tag="VM713_05830"
     CDS             2360..3337
                     /gene="hemB"
                     /locus_tag="VM713_05830"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HVH83817.1"
                     /translation="MPFPQERPRRTRQSAAWRRMVRETRLTPDALVYPLFVVPGRSVR
                     HAVESMPGIAQLSVDEAVKEAKAAAGAGVPAVLLFAAPAEKDARASAARDPRGLAAQA
                     IAAIKEACPELLVWADVCLCGATDHGHCGHVLEGGTIDNDSSVATLAEVALNYARAGA
                     DAVAPSDMMDGRVQAIRALLDREGLAATPIISYAAKYASAFYGPFREAAESAPAFGDR
                     RSYQMDPANAREALREVLTDVAEGADLVMVKPAGPYLDVIRQVRDAVRVPVVAYQVSG
                     EFSLIKAAAQRGWIDERAAVLETLTGIARAGADLIISYFAPEVARWLRT"
     gene            3322..>3957
                     /locus_tag="VM713_05835"
     CDS             3322..>3957
                     /locus_tag="VM713_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HVH83818.1"
                     /translation="MAAHMSERAGETRAAASAFLAACRRQPVAHRPIWIMRQAGRYLP
                     EYRELRAKVDFEALTRTPELAAEVTLQPLRRFELDAAILFSDIMTPLQGMGVGLTFEP
                     APVVREPIRTRAQIDALPALEPARDVPFVLETIRLVRRSVPRAAPLIGFAGAPFTLLC
                     YLVCGRPSKEFAAARTFLFAQPEDAERLLTHLADAMAAYLSAQAAAGAQALM"
BASE COUNT          515 a         1415 c         1434 g          593 t
ORIGIN      
        1 cgagcaggcg ctggaatggc tgcgcgcgca tcagtgtgcg ctcatcgtgc tggatgtgat
       61 gctgccggga attgacggct tcggcgtgtg ccgcgcgctg cgcgagcagg gcaacaacac
      121 accggtgctt tttctcaccg cgcgtggtga cccggtggac cgggtgcggg gtctggaggc
      181 cggcggcgac gattacctcg ccaagccctt ccacctggag gagttcctgc tgcgcgtgcg
      241 ggccatcctg cgccgctggg actggtatcg cagcgcctcg gcgacggtcg ctacagcggt
      301 gctgcgcttc ggtggcaacg aagtcgattt ccgcgccttt cgcgcgcgtg cgtggaatgg
      361 cgagccacag gagctcaccg agaaggaggc gatgatcctc aaagtcctgg cggagcacgc
      421 cggcgagatc gtctcgcgcg aggatctgct cgagcgggtg tggggctacg atgtgtttcc
      481 ctcgacgcgc accgtcgaca atttcatcct gcggctgcgc aagcgcttcg agcgcgatcc
      541 cgccagcccg cgccatttcc tcacggtctg gggggtcggt taccgcttcc tcgtggaggg
      601 cgagccgtga gccggccgag cttacgcatc gggacgcgcg cctcggcgct ggcgctgtgg
      661 caggcgcggc acgtcgaggc gctgctgcgc gcgcagcgcg gggcgccgcc catcgagctg
      721 gtgcacatcc gcaccagcgg cgatgcgcaa cgcgaggtgc cgctgtggca gaccgaaggc
      781 cgcgccttct tcacgcgcga gatcgacgcg gcactttccg cgggcacgat cgacgtcgcg
      841 gtgcacagcc tcaaggatct gccgaccacg ctcgctccgg gactcgcgct cggcgcggcg
      901 ctcgcgcgcg aggacccgcg cgatgcgctg ctcacgcgcg cgggcgtgtc cttgaggcag
      961 ctgccgccgc gaccgcgtat cggcacgagc agcctgcggc gccgcgcgtt cctcgcgcgc
     1021 ctccggcccg acgccgtgcc gacggagctg cgcggcaatg taccgacgcg catcgagcgg
     1081 ctcgagcgcg gcgattacga tgcgatcatt ctggccgcgg ccggactcgc ccggctgggg
     1141 ctcagtcagc acgtcagcga atacctgtcg ccagaggagt tcccgccggc ggtatcgcag
     1201 ggggtgatcg gcgtgtgtgc gcgcgctgac gacgagtcga cgctcgcgct gctgcgcgcc
     1261 ctcgatgacg cgggtgcgcg gctcgcggtg agcgccgagc gcgcactgct gcagcgcctc
     1321 gaaggaggct gccaggtgcc gctcggcgcg ctcgccacga cgcgcggcgc ggcgctcaat
     1381 ctgcaggccg cggtgtgcgc gctcgacgga tcgcgcgagc tgcgcgcgcg cggcacggcc
     1441 agcgctgctc acggcgcggt cctcacccgc gccgatgccg tggcgctcgg ggagcggatc
     1501 gccgacgagc tgctcggcgc gggcgcggcc gagctcatcg cgcacgagcg cgcggcactt
     1561 gcagtgacgg cgccatgagc agtgcctccg acacgcgccc cgtcgtggtg acccgcgcgg
     1621 aaagcgccga cggccccttg agtcgcgagc tccgcagtct cgggctcgag gtgctgtcgt
     1681 ggcccgccgt gagcgtcacg gcctccgatt cgagcgcgct ggaagacgcc atctcgcgcc
     1741 tcgagtcctt cgcctggatc gtgtttgcga gccgccacgc cgtggcggcg gtgcttgagc
     1801 gcagacctaa gccgcccgcg cagccgcgcg tcgccgccgt cgggcgcgcc accgcgctca
     1861 ctctgagcca gcgcggctgg ccggtgcacc tgacgcctcc ggaggcgaat gcggcggcgc
     1921 tgatggccgc cttcgccgcg caatggcgtc catcggacgc cggcgcccgc gtgctctacc
     1981 ccgcgagctc ccgggcgctt cccaccatcg ccgcgggctt gaggcagctc ggcgccgtcg
     2041 tgacccaggt cgaagcctat cgtaccgaag ccgcggcgct cgacgtggcc gaatgccgcg
     2101 cctggatcga gcgcggtgga ctcggcgccg tgacctttgc gagtccctcg gccgtcaccg
     2161 agctcgcgcg ggcgctcggt gaggagaact tcgcgcggct gctcgacgcg gctccgccgg
     2221 tcgcgatcgg ccccaccacc gcgcgtgagc ttgccgcgcg cgggcgcaac gtgacactcg
     2281 ccgacaccgc gaccctccac gggctcgccg tcaccactta ccgtgttatg caacagagag
     2341 cctgaagaga gagccgccca tgccattccc ccaggaaaga ccgcggcgca cccggcagtc
     2401 ggctgcgtgg cgccgcatgg tgcgcgagac gcgcctgacg cccgatgcgc tcgtctatcc
     2461 gctgttcgtc gtgcccgggc gctcggtgcg tcatgcggtc gagagcatgc cggggatcgc
     2521 acagctgtcg gtggacgagg cggtcaagga agcgaaggcg gcggccggcg ccggggtgcc
     2581 ggcggtgctg ctgttcgcgg ctcccgcgga aaaagacgcg cgcgcgagtg cggcccgcga
     2641 tccgcgcggc ctcgcggccc aggcgatcgc tgcgatcaag gaggcgtgcc ccgagctcct
     2701 cgtgtgggcg gacgtctgcc tgtgtggcgc gaccgatcac ggccactgcg gccacgtgct
     2761 ggaaggcggc acgatcgaca atgattcctc ggtcgcgacg ctcgccgagg tcgcgctcaa
     2821 ttacgcgcgt gccggagccg atgcagtggc gccgagcgac atgatggacg gccgtgtgca
     2881 ggcgattcgt gcactactcg accgcgaagg gctcgcagcg acgccgatca tctcctacgc
     2941 cgccaagtac gcgtccgctt tctacgggcc gttccgcgag gcggccgagt ccgccccggc
     3001 cttcggcgac cggcgctcct atcagatgga tcccgccaat gcgcgcgaag cgctgcgtga
     3061 ggtgctgacc gacgtcgccg agggtgctga cctcgtcatg gtgaagcccg ccgggcccta
     3121 cctcgacgtc atccgccagg tgcgcgatgc agtgcgggtg ccggtagtgg cctatcaggt
     3181 gtcgggggag ttcagcctca tcaaggcggc ggcccagcgc ggctggatcg acgagcgcgc
     3241 ggcggtgctc gagacgctca ctggaatcgc gcgcgccggc gccgacctga tcatcagcta
     3301 ctttgcgccg gaggttgcgc ggtggctgcg cacatgagcg agcgtgccgg cgagacgcgc
     3361 gcggcggcga gcgcctttct cgcggcgtgc cgccgccagc ccgtggcgca tcggccgatc
     3421 tggatcatgc gtcaggccgg ccgctacctc cccgagtacc gcgagctgcg cgccaaggtc
     3481 gacttcgagg cactcacccg cacacccgag ctcgccgccg aggtgactct gcagcccttg
     3541 cggcgcttcg agcttgatgc ggcgatcctc ttcagcgaca tcatgacgcc gctgcagggc
     3601 atgggggtgg gactcacctt cgagcctgcg cctgtggtgc gcgagccgat ccgcacgcgg
     3661 gcgcagatcg acgcgctgcc cgcgctcgag cccgcgcgcg acgtgccgtt cgtgcttgag
     3721 acgatccgcc tggtgcgccg cagcgtgccg cgcgcggcgc cgctcatcgg cttcgccggg
     3781 gcgccgttca cgctcctttg ctatctggtg tgcggccggc cgtcgaagga gtttgcggcg
     3841 gcgcgcacct tcctcttcgc acagcccgag gacgccgagc ggctgctgac gcacctcgcc
     3901 gacgccatgg ccgcttacct gagcgcgcag gccgcggccg gcgcacaggc gctcatg
//
LOCUS       DATLKL010000436         3700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_73142, whole genome shotgun sequence.
ACCESSION   DATLKL010000436 DATLKL010000000
VERSION     DATLKL010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73142"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VM713_05840"
     CDS             <1..1062
                     /locus_tag="VM713_05840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVH83819.1"
                     /translation="FVSPNVLEVMGASGSQRIRFEQCIIAAGSEPVRLPGLPADPRIL
                     DSSDALELPEFSGGLLVIGGGIIGLEMACVFDALGKRVSVVELTPQLMPGCDPDLVRP
                     LERRIRGRYQQILLGTRVSAVEPQPAGLRVSFAGEKAPAPQIFDRVLVAVGRAPNGHS
                     VGADLAGVKVSERGFIPVDRQMRTNVPHIFAIGDIAAPPMLAHKAMHEGKVAAEVAAG
                     HKRAFDARVIPSVAYTDPEVAWTGLTETEAKEKGIAYAKGSFPWVANGRSLSLGREDG
                     FTKLLFDPQTHRVLGGGIVGTNAGELISEVGLAVENGCDAADIGLTIHPHPTLSETVA
                     GAAEAFEGTLTDLYIPKKK"
     gene            complement(1099..1797)
                     /gene="nth"
                     /locus_tag="VM713_05845"
     CDS             complement(1099..1797)
                     /gene="nth"
                     /locus_tag="VM713_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HVH83820.1"
                     /translation="MHPATRAAIFRRLRAANPTPTTELVHGSPFELLVAVMLSAHTTD
                     KSVNAATSVLFPVANTPAAIAALGVEGVKPYIRSVGLYNTKAANLVALARQIVERHGG
                     EVPSDRAALEALPGIGRKTASIILNMVFGQPEIAVDTHIFRVANRTGLAPGKTPREVE
                     DGLVAGTPPEFRKDAHHWLILHGRYVCTARAPKCPTCIIRDLCEYKHKTKAPAAKRRG
                     VAAAIRRSGKRARR"
     gene            complement(1820..2386)
                     /locus_tag="VM713_05850"
     CDS             complement(1820..2386)
                     /locus_tag="VM713_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HVH83821.1"
                     /translation="MGGSSLAAFMVSAHDIDAVLPQTQCTRCGYQGCQPYAEAIAAGL
                     AEINQCPPGGAATIGALAALLGRTPLPLNPANGVAGPELVAQIDEEACIGCARCLPPC
                     PVDAIVGAGKQMHTVVMALCTGCELCVAPCPVDCIAMVPRASLAGAPPAPTPVDNRAR
                     YVAHGTRLAQRGEQRASLLAARKQAAPA"
     gene            complement(2388..>3700)
                     /gene="metG"
                     /locus_tag="VM713_05855"
     CDS             complement(2388..>3700)
                     /gene="metG"
                     /locus_tag="VM713_05855"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HVH83822.1"
                     /translation="LAALKRYFASGKARAQGERRTFEEFLAAPDTEQIHFIGKDIIYF
                     HTLFWPAMLKFAGPPYKVPSHVYVHGFITVSGEKMSKSRGTGISPLRYLDVGMNPEWL
                     RYYIAAKLNASVEDLDFNPEDFLARVNSDLIGKYVNIASRAASFITRHFGGTLRYGGD
                     TGQLTARAAAHAALVQESYESREFGKAMRDIMGVADHINQEFDARQPWVLARDAKRAA
                     ELQDVCSRALAGFKLLSVLLAPVLPAVAERVARQLFGLDRPFRWSDAAVLPERINPYE
                     HLMTRVEPRQLEALFATDAPTPSGSTTATAGGSKSPAVSPGPAPAAASGPVAATISID
                     EFRRIDLRVARIVRAEAVAGADKLLKLTLDLGTETRTVFAGIKTAYDPAALEGRLTVM
                     VANLAARKMKFGVSEGMVLAASGESPGLYLLAPDGGAAPGMKIT"
BASE COUNT          553 a         1231 c         1333 g          583 t
ORIGIN      
        1 ttcgtgagcc cgaacgtgct cgaggtcatg ggcgcaagcg gctcgcagcg catccgcttc
       61 gagcagtgca tcatcgccgc gggctccgag ccagtcaggc ttcctggcct ccctgccgat
      121 ccgcgcatcc tcgactcgag cgacgcgctc gagctcccgg agttctccgg gggcctgctg
      181 gtgatcggcg gcggcatcat cggactcgag atggcctgcg tgttcgatgc gctcggcaag
      241 cgcgtgagcg tcgtcgagct caccccgcag ctcatgcccg gttgcgatcc cgacctggtg
      301 cggccgctcg agcggcgcat ccgtgggcgc taccagcaga tcctgctcgg caccagggtg
      361 tcggccgtcg agccgcagcc cgcggggctg cgggtaagct ttgccgggga gaaggctccg
      421 gcgccgcaga tcttcgaccg ggtcctggtg gcggtcgggc gggcgcccaa cggtcacagc
      481 gtcggcgccg acctcgccgg agtgaaggtg tccgagcgcg gcttcatccc ggtggatcgg
      541 cagatgcgca ccaacgtgcc gcacattttt gcgatcggtg acattgccgc gccgccgatg
      601 ctggcgcaca aggcgatgca cgaaggcaag gtggccgccg aagtcgcggc cggccacaag
      661 cgtgccttcg atgcacgcgt gattccgtcc gtggcctaca ccgatccgga agtcgcctgg
      721 accggtctca ccgaaaccga ggcgaaggag aaggggattg cctacgccaa gggcagcttt
      781 ccgtgggtgg cgaatggccg ctcgctgtcg ctcggacgcg aggacggctt caccaagctg
      841 ctgttcgacc cgcagacgca ccgcgtgctg ggcggcggca tcgtcggcac caacgcgggc
      901 gagctgatca gcgaagtcgg gctcgcggtg gagaacggct gcgacgccgc ggacatcggc
      961 ctgacgattc acccgcaccc gaccctgtca gagaccgtcg cgggggcggc cgaggcgttc
     1021 gagggaacgc tgacagacct gtatatcccg aagaagaagt agcgacgcgc gcaggcgccc
     1081 gagcgtccgg ccagaggctc agcgccgtgc gcgcttcccg ctgcgtctaa tggcggccgc
     1141 cactcccctg cgcttcgccg ccggcgcctt cgtcttgtgc ttgtactcgc acaggtcgcg
     1201 gatgatgcag gtcggacact tcggcgcgcg cgccgtgcac acgtagcggc cgtgcaggat
     1261 cagccagtga tgcgcgtcct tgcggaactc gggcggcgtg ccggcgacga ggccatcttc
     1321 gacttcgcgg ggcgtcttgc cgggagcgag cccggtgcgg ttggcgacac ggaatatgtg
     1381 cgtatcgacc gcgatctcgg gctggccgaa caccatgttg agaatgatgc tggcggtctt
     1441 gcggccgatg cccggcagcg cttcgagcgc ggcgcgatcg gatggcacct caccgccgtg
     1501 tcgctcgacg atctgccgcg cgagcgcgac caggtttgcg gccttggtgt tgtagagacc
     1561 gacgctgcgg atgtagggct tcactccctc gacgccgagc gctgcgatgg ccgcgggcgt
     1621 gttggcgacc gggaaaagca cgctggtggc ggcgttcacg ctcttgtcgg tggtgtgcgc
     1681 cgagagcatc acggccacca gcagctcgaa cggcgagcca tgaacgagct cggtggtggg
     1741 ggtgggattc gcggctcgca gccggcgaaa gatggcagcg cgcgttgccg gatgcatggg
     1801 tcagcagggc cgcacggggt taagccggcg ctgcctgctt gcgcgcggcg aggagggacg
     1861 cgcgctgctc gccccgctgc gcgagccgcg tgccgtgggc gacgtagcgc gcgcgattat
     1921 cgacgggcgt cggcgcgggc ggcgccccgg cgaggctggc gcgcggcacc atcgcgatgc
     1981 aatcgacggg gcacggcgcg acgcacagct cgcaaccggt gcagagcgcc atcacgacgg
     2041 tgtgcatctg tttgccagcg ccgacgatcg catccaccgg acagggcggc aggcagcggg
     2101 cgcagccgat gcaggcctcc tcgtcgatct gtgccacgag ctcgggtccg gccacgccgt
     2161 tcgcggggtt gagcggcagc ggcgtgcgcc caagcagcgc tgcgagcgcg ccgatggtgg
     2221 cggcgcctcc gggcggacat tgattgatct cggcgagccc ggccgcgatt gcctcggcgt
     2281 agggctggca gccctggtac ccgcaacgcg tgcattgggt ctgcggcagg acggcgtcga
     2341 tgtcgtgcgc gcttaccatg aacgccgcga ggctcgagcc gcccacctca ggtgatcttc
     2401 atgccgggcg ccgcgccgcc gtccggcgcg aggagataga ggcccggact ctcaccgctc
     2461 gccgccagca ccataccttc cgagacgccg aacttcatct tgcgggcggc aagattggcg
     2521 accatcacgg tcagtctgcc ctcgagcgct gccggatcgt aggccgtctt gattcccgcg
     2581 aacacggtgc gggtttccgt tcccagatcg agcgtgagct tgaggagctt gtcggcaccg
     2641 gcgaccgcct cggcgcgcac gatccgtgcc acccgcaggt cgatgcggcg gaactcgtcg
     2701 atggagatgg tggcagccac ggggccggac gccgccgcgg gcgcggggcc ggggctcacc
     2761 gcggggctct tcgacccgcc cgccgtcgcg gtggtgctgc cggagggcgt cggcgcatcg
     2821 gtagcgaaca gcgcctcgag ctgccgcggc tcgacccgcg tcatgagatg ctcgtaaggg
     2881 ttgatgcgct cgggcagcac cgcggcatcg ctccagcgga acggccggtc gagtccgaag
     2941 agctgccgcg cgacccgctc ggccaccgcc ggcagcaccg gcgcgagcag tacactgagg
     3001 agcttgaagc cggcgagcgc acgcgagcac acgtcctgca gctccgcggc gcgtttggca
     3061 tcgcgcgcca gtacccaggg ctggcgcgca tcgaactcct gattgatgtg gtcggctacg
     3121 cccatgatgt cgcgcatggc cttgccgaac tcgcggctct cgtagctctc ctgcaccaac
     3181 gcggcgtgcg ccgccgcccg cgcggtaagc tgtccggtat caccgccgta gcgcagcgtg
     3241 ccgccgaaat gccgggtgat gaagctcgcc gcgcggctcg cgatgttgac gtacttgccg
     3301 atgagatcac tgttgacgcg cgcgaggaag tcctcggggt taaagtcgag gtcctcgacg
     3361 ctggcgttga gcttggccgc aatgtaatag cgcagccact cggggttcat gccgacatcg
     3421 agatagcgca acgggctgat gccggtgccg cgcgacttcg acatcttctc gccggagacg
     3481 gtgatgaagc cgtgcacgta cacgtggctc ggcaccttgt acggcgggcc ggcgaacttg
     3541 agcatcgccg gccagaacag cgtgtggaaa tagatgatgt ccttgccgat gaaatggatc
     3601 tgctcggtgt cgggcgccgc caggaactct tcgaaggtgc gccgctcacc ctgcgcgcgc
     3661 gccttgccgc tcgcgaagta cctcttgagc gcagccaggt
//
LOCUS       DATLKL010000437         1972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_208187, whole genome shotgun sequence.
ACCESSION   DATLKL010000437 DATLKL010000000
VERSION     DATLKL010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208187"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..954)
                     /gene="kdsA"
                     /locus_tag="VM713_05860"
     CDS             complement(109..954)
                     /gene="kdsA"
                     /locus_tag="VM713_05860"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HVH83823.1"
                     /translation="MTSPHHVRVGDLVIGNDRPLALIAGPCALESRQHALEMSQAIKE
                     IADRLGIGLIYKTSFDKANRTSIDSPRGIGLSESLPILAEVRERWGIAVLTDVREPGQ
                     CAIVAQAADVLQIPAFLCRQTDLLVAAARTGRAVNVKKGQFLAPWDMANVAAKITHEG
                     NENVLLTERGVSFGYNTLVSDMRALPILARMGFPVVFDATHSVQQPGGQGTRSGGQRE
                     FVAVLARAAVAVGVAAVFMEVHQDPDRAPSDGPNMIKLKDLPAILEILLAHDRVTKKA
                     KPVPA"
     gene            complement(1066..>1972)
                     /locus_tag="VM713_05865"
     CDS             complement(1066..>1972)
                     /locus_tag="VM713_05865"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HVH83824.1"
                     /translation="TIYEVPLRYHAEGFDTQVIKHFGIRQETEPDLDRWQDIVERVRR
                     PEGEVTIAVVGKYTHLLDSYKSLAEALSHGGIANHVRVNMNWIDSEIFEHDDTIRRLE
                     GVHGILVPGGFGERGTEGKIQAVKFARERKVPYFGICFGMQMAVIEVARHIAGLKGAG
                     STELGPAEHPVVGLLTEWMRGNVLERRAIGDNLGGTMRLGAYDCMLAKGSIVHRAYGR
                     DRISERHRHRYEVNINHKPQLERAGLKFSGMSPDGELPEIIEIPGHPWFVGVQFHPEL
                     KSKPLEPHPLFTSFIKAAVDQSRLV"
BASE COUNT          318 a          641 c          655 g          358 t
ORIGIN      
        1 tgattgcgct catgcagggc tctcccggcc tgccgatcgg gctggccaca gatatccgcg
       61 cggtggtgcc gttgcaaggc ggagcggggc atgcattgca ccgctcgctt aggccgggac
      121 cggctttgcc ttcttcgtaa cgcggtcgtg cgcgagaaga atctcaagga tggctggcaa
      181 gtctttgagt ttgatcatat tggggccgtc ggatggcgca cggtcgggat cctggtgaac
      241 ctccatgaag acagcggcga cgccgaccgc gaccgccgcg cgggccagca cggccacgaa
      301 ctcccgctga ccgccgctcc tcgtcccctg cccgcccggt tgctgcaccg agtgcgtagc
      361 gtcgaacact accgggaagc ccatccgcgc caggatcggc agcgcccgca tgtcagaaac
      421 gagcgtgttg tagccgaacg agacgccccg ctcggtcaag agcacgttct cgttcccctc
      481 atgggtgatc tttgccgcga cgttcgccat gtcccagggc gcgaggaact ggcccttctt
      541 gacattgacg gcgcggccgg tgcgcgcggc ggcaacgagc aggtcggtct gccggcagag
      601 gaaggcggga atctgcagca cgtcggcggc ctgggcgacg atcgcgcact gccccggctc
      661 acgcacgtcg gtcagcacgg cgatccccca ccgctcgcgc acctcggcca gaatcggcaa
      721 gctttcggag agcccgatgc cgcgcgggct gtcgatcgag gtgcggttgg ccttgtcgaa
      781 ggaggtcttg tagatgagcc cgatgccgag cctatccgcg atttccttga ttgcctggga
      841 catctccagc gcgtgctggc gggattccag cgcgcagggg cccgcgatga gagcgagtgg
      901 tcggtcgttg ccgatgacga gatcgccgac ccgaacgtga tgaggtgacg tcatcgagcc
      961 cgctcaaaga ccttcgcacc cgcgacccct cgcccctccg tcggctcgcg tcgagggcgg
     1021 ccgggggtag ggaccgatga aactatcagc ccgtcgcgcg cgaggctaca ccagtcggct
     1081 ctggtccacc gccgccttga tgaaggaggt gaacagcgga tgcggctcga ggggcttcga
     1141 cttgagctcc gggtggaatt ggacgccgac gaaccatgga tggcctggga tctcaatgat
     1201 ctcgggcagc tcgccgtccg gtgacatgcc cgagaatttg aggcctgcgc gctcgagctg
     1261 gggcttgtgg ttgatgttga cctcgtaacg atgtcggtgt cgctcgctga ttcggtcgcg
     1321 tccgtaggcc cggtgcacga tgctgccctt ggcgagcatg cagtcataag cgccaagccg
     1381 catggtgccg cccagattgt caccgatcgc gcgccgctcg agcacgttgc cgcgcatcca
     1441 ctcggtcagg aggccgacga ccggatgctc ggccgggccg agctcggttg agccggcgcc
     1501 cttgagccca gcgatgtggc gcgccacctc gatcactgcc atctgcatgc cgaagcagat
     1561 gccgaaatag ggcaccttgc gctcgcgcgc gaacttcacc gcctgaatct ttccctcagt
     1621 gccgcgctcg ccgaagccgc ccggcacaag gatgccgtgc acgccctcga gtcggcggat
     1681 ggtgtcgtcg tgctcgaaga tctccgaatc gatccagttc atgttgacgc gcacatggtt
     1741 ggcaatgccg ccatggctca gcgcctcggc aagcgatttg tacgaatcga ggaggtgcgt
     1801 gtacttgccg accacggcga tcgtgacctc cccctctggt ctgcgcacgc gctcgacgat
     1861 gtcctgccag cggtcgaggt cgggctccgt ttcttgcctg atgccgaaat gcttgatcac
     1921 ctgggtgtcg aacccctcgg catggtagcg cagcggcacc tcgtagatgg tg
//
LOCUS       DATLKL010000438         2131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_163078, whole genome shotgun sequence.
ACCESSION   DATLKL010000438 DATLKL010000000
VERSION     DATLKL010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163078"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2131
                     /locus_tag="VM713_05870"
     CDS             <1..>2131
                     /locus_tag="VM713_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HVH83825.1"
                     /translation="RVYTPEDGRRLGLTGMIDDVFTRLAAVEKPPYEFRPLNARDHGL
                     VARTISVLEGAAGAAEPQAEQIRRALGRSRHRAPVIGITGTGGAGKSSLTDELLARLT
                     RALPKLQIAVVAMDPTRRRSGGALLGDRIRMNSLSSDNVYMRSLATRRAHLATSAVLR
                     EVIQLYQACGFDLVLIETAGIGQSDTEIVDMVDLSLYVMTSEYGAASQLEKIDMLDYA
                     DFIVLNKSEKRGAEDSLRDVRKQWRRNHPERAKLGDAELPVFPTIASRFNDPGVNRLF
                     ASLCATLAARHIGTGSWQLSDAGPTELSARAPLIPGSRTRYLAEIAHAGRAARARAEE
                     QCQAARRAYGLYESLKALHDARLPGPLEAYPPEALEERSGDATRRELRTAYNRALEEI
                     GAEGQAELKAWPERARSASDATYSYKVRERLVKGENYTESLARSRIPKLAVPKVRDWG
                     ELLHFLLLENLPGAYPYTGGVYPYRREEEDPTRMFAGEGGPERTNRRFHYLAHGHGAA
                     RLSTAFDSTTLYGEDPDTRPDVYGRTGNSGVSIATLDDMKKLYSGFDLCSPSTSVSMT
                     INGPAPMLLAMFMNTAIDQRVERYLKAEGRWAAASERIAAGNAAAAARGAAPPTYKGA
                     LPAHHDGAGLALLGVTGDELIEPEVYERIRAETLEAVRGTVQADILKEDQAQNTCIFS
                     TEFALRMMGDVQQYFSEHRVRNFYSVSI"
BASE COUNT          347 a          763 c          728 g          293 t
ORIGIN      
        1 cgggtctaca cacccgagga cggccggcgg cttggcctta ccggcatgat cgacgacgtg
       61 ttcacgaggc tcgctgcggt cgagaagccg ccgtatgaat tccgtcctct caatgcgcgc
      121 gatcacggcc tcgtggcgcg caccatttcc gtgctcgagg gcgccgcggg agccgcggag
      181 ccccaggccg agcagatccg ccgcgcgctg gggcgttcgc gccaccgggc accggtgatc
      241 ggcatcaccg gcacaggggg cgcgggcaag tcgagcctca ccgacgagct gctcgcgcgc
      301 ctgacgcgcg ccctccccaa gctgcagatc gccgtggtgg cgatggaccc gacgcggcgg
      361 cgcagtggcg gggcgctgct cggggaccgc atccgcatga acagcctctc gtccgacaac
      421 gtctacatgc gctcgctcgc cacgcggcgt gcgcacctcg cgacctcggc ggtgttgcgc
      481 gaggtgatcc agctgtacca ggcgtgcggc ttcgacctgg tgctgatcga gaccgccggc
      541 atcggccaat cggacaccga gatcgtcgac atggtcgacc tgtcgctgta cgtcatgacg
      601 agcgagtacg gcgccgccag ccagctcgag aagatcgaca tgctcgacta cgccgacttc
      661 atcgtgctca acaagagcga gaagcgcggc gcggaggaca gtctgcgcga cgtgcgcaag
      721 caatggcggc gcaaccatcc cgagcgcgcc aagctcggcg atgccgagct gccggtattc
      781 ccgacgatcg cgagccgctt caacgacccg ggcgtgaacc ggctgtttgc gagcctgtgc
      841 gccacgctcg ccgccaggca catcggcacc ggctcgtggc agctcagcga cgccggcccc
      901 accgagctca gcgcgcgcgc cccgctcatt cccgggtcgc gcacccgcta tctggccgag
      961 attgcgcacg cgggccgagc cgcgcgcgcg cgcgccgagg agcagtgtca ggccgcgcgc
     1021 cgcgcctacg gcctgtacga gtcgctgaag gcgctgcacg acgcacggct gccggggcca
     1081 ctcgaggcgt atccgccgga agctctcgaa gagcgctcgg gcgatgccac gcggcgcgag
     1141 ctgcgcacgg cctacaaccg cgcgctcgaa gagatcggcg ccgagggcca agctgagctc
     1201 aaggcctggc ccgagcgggc gcgctcggcg agcgacgcaa cctactccta caaggtgcgc
     1261 gagcggctcg tgaagggtga gaactacacc gagagcctcg cgcgctcgcg gatcccgaag
     1321 cttgccgtgc cgaaggtacg cgactggggc gagctgctgc atttcttgct gctggagaac
     1381 ctccccggcg cctatcccta caccggcggc gtctatccct atcggcgcga ggaggaggat
     1441 ccgacccgca tgttcgccgg ggagggggga cccgagcgca ccaaccggcg cttccactac
     1501 ctcgcgcacg ggcacggcgc ggcgcgcctt tcgaccgcgt tcgattcgac caccctctac
     1561 ggagaggatc cggacacgcg tcccgacgtc tacggccgca ccggcaactc cggtgtctcg
     1621 atcgccaccc tcgatgacat gaagaagctc tactcgggct tcgacctgtg ctcgccctcg
     1681 acctcggtgt ccatgacgat caacgggccg gcgccgatgc tgctcgccat gttcatgaac
     1741 acggccatcg accagcgggt cgagcgctac ctcaaggccg agggccgctg ggccgcggcg
     1801 agtgagcgga tcgccgccgg caacgcggcg gccgcggcgc gcggcgcggc gccgcccacc
     1861 tacaagggcg cgctgcccgc gcaccacgac ggcgcggggc tcgcgctcct cggggtgacg
     1921 ggagacgagc tcatcgagcc tgaggtgtac gagcgcatcc gtgccgagac gctcgaagcg
     1981 gtgcgcggca ccgtgcaggc cgacatcctc aaggaagacc aggcgcagaa cacctgcatc
     2041 ttctcgacgg agttcgcgct gcgcatgatg ggggacgtgc agcagtactt cagcgagcac
     2101 cgggtgcgca acttctactc cgtgtcgatc t
//
LOCUS       DATLKL010000439         1631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_95506, whole genome shotgun sequence.
ACCESSION   DATLKL010000439 DATLKL010000000
VERSION     DATLKL010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95506"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VM713_05875"
     CDS             <1..566
                     /locus_tag="VM713_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVH83826.1"
                     /translation="LDVTDGASVIRCVAAATALGPLTGLVNCAGVVRETRLDEMPEVE
                     AAAMWEINVMGSARVTRACLPHLTDGASVVHIGSVAGAVGRFAGTGIYSASKAALGGL
                     ARVGACELAARGIRVNTVAPGFIDVPMAPAWDRMSGGEAALAAQVPLGRLGRTDEVAE
                     VVEFLLSARASFVTGAVIPVDGGTAAW"
     gene            619..>1631
                     /locus_tag="VM713_05880"
     CDS             619..>1631
                     /locus_tag="VM713_05880"
                     /inference="COORDINATES: protein motif:HMM:NF018275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HVH83827.1"
                     /translation="MTAAERERANLAAHRRMCEAEPVLVGVAAARDAVPGMTDRTILT
                     SGPAVPFARYSGGQRAALVYAALYEGLAGSPADAERRMDEGDIVIGACHDHGCVGSVA
                     GVFTASMPVFVVRDQVHGTTGRCNFYEGESRKRLNYGAYDDGVRDQLRYIEKVIAPTI
                     GAAVERAGGIPLKPIMRRAVHMGDELHSRNTAATLLFGRELFPHLLAAGPDRRDDVLQ
                     TLGYLSASDYFFLRLSMASGKAVADAARGIEASSVVTAMSITCRGVSIRVSGLGDEWF
                     DGPFPDVSAKLFDGFTPDDIEWIGGGSLINETVGLGGFAQAAAFPLQNYQGGSPEAMI
                     EMNL"
BASE COUNT          221 a          552 c          642 g          216 t
ORIGIN      
        1 cgctcgacgt caccgacggg gcgagcgtca tccggtgcgt ggcggccgcg acggcgctcg
       61 ggccgctgac cggcctggtg aactgcgccg gggtggtgcg cgagacgcgg ctcgacgaga
      121 tgcctgaggt cgaggccgcg gcgatgtggg agatcaacgt gatgggcagc gcccgggtga
      181 cccgggcctg cctgccccac ttgaccgacg gcgcctcggt cgtgcacatc ggcagcgtcg
      241 ccggcgcggt cggcaggttc gccgggaccg gcatctactc ggcctcgaag gccgcgctcg
      301 gcggcctggc gcgggtcggg gcctgcgagc tggcggcgcg cggcatccgg gtcaacacgg
      361 tcgcgcccgg cttcatcgac gtgccgatgg ccccggcctg ggaccgcatg tcgggcggcg
      421 aggccgcgct ggcggcgcag gtgccgctcg gccggctcgg ccggaccgac gaggtcgcgg
      481 aggtcgtgga gttcctgctg tcggcgcggg cgtccttcgt caccggcgcg gtgatcccgg
      541 tggacggcgg gacagcggcg tggtgagcga gccggagcgc ctccaggcgc cccgcaggcg
      601 agcgaaaagg cagacacagt gaccgccgcc gagcgcgagc gggccaacct ggctgcccac
      661 aggcggatgt gcgaggccga gcccgtgctg gtcggggtgg cggcggccag ggacgcggtg
      721 cccggcatga ccgaccggac gatcctgacg tcggggcccg cggtgccgtt cgcgcggtac
      781 agcggcggac agcgggccgc gctggtctac gccgccctgt acgagggcct ggccggcagc
      841 ccggccgacg ccgagcggcg gatggacgag ggcgacatcg tcatcggggc gtgccacgac
      901 cacggctgcg tggggtcggt ggccggcgtg ttcaccgcgt cgatgccggt gttcgtggtg
      961 cgcgaccagg tgcacggcac caccggccgc tgcaacttct acgaggggga gtcgcgcaag
     1021 cgactgaact acggcgccta tgacgacggc gtgcgcgacc agttgcgtta catagaaaag
     1081 gtcatcgctc ccacgatcgg ggccgcggtc gagcgggcgg gcggcatccc gctcaagccg
     1141 atcatgcgcc gcgccgtgca catgggcgac gaactgcaca gccgcaacac cgcggccacg
     1201 ttgctgttcg gccgggagct gttcccgcac ctgctcgccg ccggcccgga ccgccgcgac
     1261 gacgtgctgc agaccctggg ctacctgtcc gccagcgact acttcttcct gcggctgtcg
     1321 atggccagcg gcaaggcggt ggccgacgcc gccaggggca tcgaggccag cagcgtcgtg
     1381 accgcgatga gcatcacctg ccggggcgtc tcgatccggg tcagcgggct cggcgacgag
     1441 tggttcgacg ggccgtttcc cgacgtgtcg gcgaagctgt tcgacgggtt cacgccggac
     1501 gacatcgagt ggatcggcgg ggggagcctg atcaacgaga ccgtggggct cggcggattc
     1561 gcccaggcgg cggcgttccc gctgcagaac taccagggcg gatcgcccga ggccatgatc
     1621 gagatgaacc t
//
LOCUS       DATLKL010000440         1772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_16345, whole genome shotgun sequence.
ACCESSION   DATLKL010000440 DATLKL010000000
VERSION     DATLKL010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16345"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VM713_05885"
     CDS             complement(<1..286)
                     /locus_tag="VM713_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVH83828.1"
                     /translation="MHGLWLPGEESLVLKHHLAQEFALELHWFRYFGASESMSDITAR
                     LAAFVRELKSAEVHFVGHSLGGLVLYRFLECFPDQPPGRAVFLGVPCVASR"
     gene            complement(344..>1772)
                     /gene="aspS"
                     /locus_tag="VM713_05890"
     CDS             complement(344..>1772)
                     /gene="aspS"
                     /locus_tag="VM713_05890"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HVH83829.1"
                     /translation="GEEVRLRYRYLDLRREVMSERLRLRHRLTRSMRTYLDGHGFVDL
                     ETPMLTKATPEGARDYLVPSRTHPGKFFALPQSPQIFKQLLMIAGFDRYYQIVRCFRD
                     EDLRADRQPEFTQLDIETSFLSQDEIMAMMEGLVRHFFREVLDTALPEPFPRMTYAEA
                     MRRYGSDKPDLRIPLELTDVADLVRDCDFKVFAGPAKDPGGRVAALRVPGGGALARSA
                     IDECTAFVARYGAKGLAYIKVNERTRGRDGLQSPIIKFPSDAALAGILERTQAADNDL
                     VFFGADRAAVVNDALGALRLKLGHDLKMVASGWRPLWVFDFPMFEWDAEAKRWVAMHH
                     PFTSPKNPDPAALRAEPAVALAKAYDMVLNGSEIGGGSVRIHRQEMQSTVFELLGIDA
                     EEAQKKFGFLLDALRFGAPPHGGIAFGLDRLAMLMAGADSIREVIAFPKTQTAADLLT
                     DAPTEVSEAQLRELHVRVRVPPAAP"
BASE COUNT          300 a          614 c          600 g          258 t
ORIGIN      
        1 cacggctcgc cacgcacggc accccgagga acaccgcacg gccgggcggc tggtcgggga
       61 agcactcgag gaaccggtag agcaccagtc ccccgaggct atgacccacg aaatgcactt
      121 cggcgctctt gagctcgcgc acgaacgcgg cgagccgcgc ggtgatgtcg ctcatcgact
      181 cggacgcgcc gaagtagcgg aaccagtgca gctcgagcgc gaactcctgc gcgaggtggt
      241 gcttgaggac gagcgactcc tcgcccggca gccacaggcc atgcacgtag acgacgtgcg
      301 cgatgggcgg ctcctcgggc gtgcgcgctg acaggagcgc ggcttaaggc gcagcgggcg
      361 gcacgcgcac ccgcacgtgc agctcgcgca gctgcgcctc gctcacttcg gtcggcgcat
      421 cggtgagcag atcggcggcg gtctgcgtct tggggaaggc gatcacctcg cggatgctgt
      481 cggcgcccgc catcagcatc gctaggcgat cgaggccgaa ggcgatgccg ccgtggggcg
      541 gcgcgccgaa cctgagcgca tcgagcagga acccgaactt cttctgcgct tcctccgcgt
      601 cgatgccgag cagctcgaac accgtcgact gcatctcctg gcggtgaatg cgcaccgagc
      661 cgccgccgat ctcggagccg ttcagcacca tgtcataggc cttggcgagc gccaccgccg
      721 gctcggcgcg cagtgccgcc ggatccggat tcttcggaga cgtgaacggg tgatgcatgg
      781 ccacccagcg cttggcctcc gcgtcccatt cgaacatcgg aaaatcgaac acccacagcg
      841 gacgccatcc gctcgcgacc atcttcaggt cgtggccgag cttcaggcgc agcgccccca
      901 acgcatcgtt gacgaccgcg gcgcggtcgg cgccgaagaa caccaggtcg ttgtcggccg
      961 cctgcgtgcg ctcgaggatg cccgcgagag ccgcatcgct cgggaacttg atgatcggcg
     1021 actgcagccc gtcgcgcccg cgggtgcgct cgttcacctt gatgtaggcg aggcccttcg
     1081 cgccatagcg cgcgacgaac gccgtgcact catcgatcgc cgagcgcgcc agcgcgccgc
     1141 cacccggcac acgcagcgcc gcgacccgtc ccccagggtc cttcgccgga cctgcgaaca
     1201 ctttgaagtc gcagtccctc acgaggtcgg cgacgtcggt aagctcgagc ggaatgcgca
     1261 ggtccggctt atcggagccg tagcggcgca tcgcctcggc gtaagtcatg cgcgggaagg
     1321 gttccgggag cgcggtgtcg agcacctcgc ggaaaaaatg gcgcaccagg ccctccatca
     1381 tcgccatgat ctcgtcctgc gagaggaacg aggtctcgat gtcgagctgg gtgaactcgg
     1441 gctgccggtc cgcgcgcagg tcctcgtcgc ggaagcagcg cacgatctgg tagtagcggt
     1501 cgaagcccgc gatcatcagc agctgcttga agatctgcgg cgactgcggc agcgcgaaga
     1561 acttgcccgg atgcgtgcgg ctcggcacca gataatcccg cgccccctcg ggggtcgcct
     1621 tggtgagcat cggcgtctcg agatcgacga agccgtgccc atcgaggtac gtgcgcatcg
     1681 agcgggtgag gcggtggcgc agccgcagcc gctcactcat cacctcgcga cgcagatcga
     1741 gatagcggta gcgcaggcgc acctcctcgc cg
//
LOCUS       DATLKL010000441         1672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_50246, whole genome shotgun sequence.
ACCESSION   DATLKL010000441 DATLKL010000000
VERSION     DATLKL010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50246"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="VM713_05895"
     CDS             complement(<1..1646)
                     /locus_tag="VM713_05895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HVH83830.1"
                     /translation="MLTELTAKRTADLIKGAAEAPAGSDARKIGDYYASFMDEARIEK
                     LGLEPLRAALARIDTIRDRAQLAQALGTTLRADVDVLNNTNLHTQNLFGLWVAQDLDD
                     PRSYSPFLLQGGLGMPDRDYYLSQSAPMEAIREKYQAHIATVLELAGVADARTRAARI
                     FALERRIAEAHVSRADSEDVRKGNNHWSRADFDTRAPGLDWQAFFAAADLAQRPRFVV
                     WQPGAVSAISSLVASEPLDTWKDYLRFHALERASPYLPKAFVQENFEFHSHVLSGTPA
                     LRERWKRAVDDTNDALGDAVGKLYVARYFPASDKARVEAMVRNLVAAFAARIDRLEWM
                     APETRRQAKAKLATLKVGLGYPDTWRDYSGLEVVRGDALGNAERASLFEYRHSLAKLG
                     APVDRSEWCLTPQTVNALNLPAMNALNFPAAILQPPFFDPRRDPVLDYGAIGAVIGHE
                     ISHSFDDQGALFDADGRLRNWWTKDDFAHFEAAGKRLAEQFDRYHPFPDVAVNGRQTL
                     SENIADVAGLADALDAWRLQLAGKPAAGAAGFSAEQLFFL"
BASE COUNT          244 a          549 c          605 g          274 t
ORIGIN      
        1 ctgaggaaga acagctgctc ggcgctgaag cccgcggcgc ccgcggccgg tttgccggcg
       61 agctgcaggc gccaggcgtc gagcgcgtcg gcgaggccgg cgacgtcggc gatgttctcg
      121 ctgagcgtct ggcggccatt caccgcgacg tccggaaacg gatggtaccg gtcgaactgc
      181 tcggcgaggc gcttgccggc ggcctcgaag tgcgcgaagt cgtccttggt ccaccagttg
      241 cgcagccgcc cgtcggcatc gaagagcgcc ccctggtcgt cgaagctgtg gctgatctcg
      301 tgaccgatca cagcgccgat cgcgccgtag tcgagcaccg gatcgcgccg tgggtcgaag
      361 aacggcggct gcaggatcgc cgccgggaag ttcagcgcat tcatcgccgg caggttcagc
      421 gcgttgactg tctgcggcgt caggcaccat tcggagcgat cgaccggtgc tccgagcttg
      481 gcgagactgt gccggtactc gaagaggctc gcccgctcgg cgttgccgag cgcatcgccg
      541 cgcaccacct cgagccccga atagtcgcgc cacgtgtcgg ggtagccgag gccaaccttg
      601 agtgtcgcga gcttcgcttt cgcctggcgc cgggtctcgg gcgccatcca ctcgagccgg
      661 tcgatgcgcg cggcgaaggc cgcgacgaga ttgcgcacca tcgcctcgac gcgggccttg
      721 tccgaggccg ggaaatagcg cgcgacatac agcttcccga cggcatcgcc gagggcatcg
      781 ttggtgtcgt cgaccgcgcg cttccagcgc tcgcgcagcg ccggcgtgcc ggagagcaca
      841 tggctgtgga actcgaaatt ctcctggacg aacgccttcg gcaggtaggg cgaggcccgc
      901 tcgagggcgt gaaagcgcag gtagtccttc caggtgtcga gcggctcgct cgcgaccagc
      961 gaggagatcg cgctgaccgc gcccggctgc cagacgacga agcgcggccg ctgcgcaaga
     1021 tccgcggcgg cgaagaatgc ctgccagtcg agccccggag cgcgcgtgtc gaaatcggct
     1081 cgcgaccaat gattgttgcc cttcctgacg tcctcggagt cggcgcggct tacgtgcgct
     1141 tccgcaatcc gccgctcgag ggcgaagatt cgtgcggccc tggtgcgtgc gtcggcgacg
     1201 ccggccagct cgagcaccgt ggcgatgtgc gcctggtatt tctcgcggat ggcctccatc
     1261 ggcgccgact ggctgaggta gtagtcgcga tccggcatcc cgagtccgcc ctgcagcagg
     1321 aacggcgagt aggaccgggg gtcatcgaga tcctgcgcga cccacaggcc gaagagattc
     1381 tgggtatgga ggttggtgtt gttgagcacg tcgacgtcgg cgcgcagcgt ggtgccgagc
     1441 gcctgcgcca gctgggcgcg atcgcggatg gtgtcgatgc gcgcgagtgc ggcgcgcaac
     1501 ggctcgagcc cgagcttctc gatgcgcgcc tcgtccatga agctcgcgta gtagtcgccg
     1561 atcttgcgcg cgtcggatcc ggcaggggcc tcggccgcgc ccttgatcag atcggcggtg
     1621 cgctttgcgg tcagctcggt cagcacctcg ccggtgccgt aggtgctgcg gt
//
LOCUS       DATLKL010000442         2395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_129396, whole genome shotgun sequence.
ACCESSION   DATLKL010000442 DATLKL010000000
VERSION     DATLKL010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129396"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VM713_05900"
     CDS             complement(<1..1391)
                     /locus_tag="VM713_05900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HVH83831.1"
                     /translation="MAHLLSILIWLPIVAGLGVLLLGDRRIGAGRWLSLLASLVTLIL
                     TVPLWGEFDTGTAALQFVEKLPWIPRFQAYYALGIDGISLPLIVLTAFMTVPVVIAGW
                     TVIESRPAQYYAAFLIMEGLMIGVFSASDALLFYFFWEAMLIPMFLIIGIWGGPRRVY
                     ATIKFFLYTFLGSVFMLAALIYMYVKAGDYSIASLQALPLTLVEQRWIFLALMLAFAV
                     KVPMFPVHTWLPDAHVEAPTGGSVILAAIMLKMGGYGFLRFSLPIAPDASRELDLLVI
                     ALSLIAVIYIGFVALVQQDMKKLIAYSSIAHMGFVTLGAFVIYEIVRATGSSRGAGMG
                     LDGAMVQMISHGLISGALFLCVGVLYDRVHSRQIADYGGVVNTMPVFASFMVLFALAN
                     TGLPGTSGFVGEFLVIIASFRASFWYAALAALTLILGACYTLWLVKRVIFGAVATPKV
                     AALEDLNPREFVVL"
     gene            complement(1402..>2395)
                     /locus_tag="VM713_05905"
     CDS             complement(1402..>2395)
                     /locus_tag="VM713_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVH83832.1"
                     /translation="THAFFKALLFLAAGSVIIALHHEQDMRRMGGLAKYMPITAVTCW
                     IGALALIGTPFFSGFYSKDAIIEAVGASHRWGARFAYPCVLLGVFVTALYTFRLLFLT
                     FHGPERFREAAGHAARDDHEDSHDANHGHDDDGGHGGDPRESPWVVTVPLIALAIPSV
                     LIGALTVAPVLFGSYFGPSIFVLPANDVTAKVGEDFSGAVSFALHGFLAPPFWFAAAG
                     AATAWLFFLKRPALADQAARRLRLMYRILCEKYYFDWFDEKVLATLTRAVGFGLWKGG
                     DEALIDGAMVNGTADSVSWFGGVLRRVQSGYLYSYAFWMVIGLAVLLGWYLTHP"
BASE COUNT          499 a          764 c          818 g          314 t
ORIGIN      
        1 agcaccacga actcacgcgg gttcaagtcc tcgagcgcgg cgaccttggg tgtggcgacg
       61 gcgccgaaga tgacgcgctt caccagccag agcgtgtagc aggcgccgag aatcagcgtg
      121 agcgcggcga gcgcggcgta ccagaagctc gccctgaagc tcgcgatgat caccaggaat
      181 tcgccgacga acccggaggt gccgggcaac cccgtgttgg cgagggcgaa cagcaccatg
      241 aagctggcga ataccggcat ggtgttgacc acgccgccgt agtcggcgat ctggcggcta
      301 tgcacccggt cgtagagcac gccgacgcac aggaacagcg ccccggagat gagcccgtgg
      361 gagatcatct gcaccatcgc gccgtcgagc cccatgccgg cgccgcggct cgagccggtc
      421 gcgcgcacga tctcgtagat cacgaaggcg ccgagcgtca cgaagcccat gtgcgcgatc
      481 gaggagtagg caatcagctt cttcatgtcc tgctgcacca gcgccacgaa gccgatgtag
      541 atcaccgcta tgagtgacag ggcgatcacc agcagatcca gctcgcggct cgcatccggc
      601 gcgatcggca acgagaagcg caggaagccg tagccgccca tcttcagcat gatggccgcg
      661 aggatcaccg agccgccggt gggcgcctcg acgtgcgcat ccggcagcca ggtgtgcacc
      721 gggaacatcg gcaccttgac ggcgaaggcg agcatgagcg cgaggaagat ccagcgctgc
      781 tcgacgagcg tgagcggcag cgcctgcagg ctcgcgatcg agtagtcgcc cgccttgaca
      841 tacatgtaga tgagcgcggc cagcatgaac accgagccga ggaaggtgta gaggaagaac
      901 ttgatggtgg cgtagacgcg gcgcggaccg ccccagatgc cgatgatgag gaacatcggg
      961 atcagcatcg cttcccagaa gaagtagaag agcagcgcat cgctcgccga gaacaccccg
     1021 atcatgagtc cttccatgat gaggaaggcg gcgtagtact gcgccgggcg gctctcgatc
     1081 accgtccagc cggcgatcac gaccggcacg gtcatgaagg cggtgagcac gatgagcggc
     1141 agcgagatgc cgtcgatgcc gagtgcgtag tacgcctgga agcgcgggat ccacggcagc
     1201 ttctcgacga actgcagcgc cgccgtgccg gtgtcgaact ccccccacag cggcaccgtg
     1261 aggatgagcg tcacgagcga ggcgagcagc gacagccagc gccccgcgcc gatgcgccgg
     1321 tcgccgagca gcagcacacc taagcccgcg acgatcggca gccagatgag gatggagagc
     1381 agatgtgcca tgcgcgggtc tttatggatg ggtcaggtac cagccgagga gcaccgcgag
     1441 cccgatgacc atccagaagg cataggagta gagatagccg ctctgcaccc gccgcagcac
     1501 gccgccgaac cagctgacgc tgtccgcggt gccgttgacc atggcgccgt cgatcagtgc
     1561 ctcgtcaccg cccttccaga gaccaaagcc gaccgcgcgc gtgagcgtcg ccaggacctt
     1621 ctcgtcgaac cagtcgaagt agtacttctc gcacaggatc cggtacatga ggcgcaagcg
     1681 tcgcgcggcc tggtcggcca gcgccggacg cttgaggaaa aacagccacg cggtcgcggc
     1741 cccggcggcc gcgaaccaga acggtggcgc gagaaatccg tggagcgcaa aggagacggc
     1801 accgctgaaa tcctcaccga ccttggccgt aacatcgttg gcgggcagca caaagatcga
     1861 ggggccgaag tagctgccga agagcaccgg agccaccgtg agggccccga tcagaaccga
     1921 agggatcgcg agcgcgatga gcggcaccgt caccacccag gggctctccc gggggtcgcc
     1981 gccgtgcccg ccgtcatcat cgtgtccatg attggcgtca tgcgagtcct cgtgatcgtc
     2041 acgcgccgcg tgcccggccg cctcgcggaa gcgctccggg ccatggaagg tgaggaacag
     2101 caggcggaag gtgtagagcg cggtgacgaa tacgccgagc agcacgcacg ggtaggcaaa
     2161 gcgcgcgccc caccggtggg acgcgccgac ggcctcgatg atcgcatcct tggagtagaa
     2221 gccggagaag aacggtgtgc cgatgagagc cagcgcgccg atccagcagg tcaccgcggt
     2281 gatcggcatg tatttcgcga gcccgcccat gcggcgcatg tcctgctcgt ggtgcagggc
     2341 gatgatcacc gagccggccg cgaggaacag cagtgccttg aagaaggcat gcgtc
//
LOCUS       DATLKL010000443         2192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_73159, whole genome shotgun sequence.
ACCESSION   DATLKL010000443 DATLKL010000000
VERSION     DATLKL010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73159"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1275
                     /locus_tag="VM713_05910"
     CDS             64..1275
                     /locus_tag="VM713_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HVH83833.1"
                     /translation="MLTESEPHYRRPLLPTPFHERARRLSQVDSFVPWAGYTTVDVFT
                     TVEQEYFAIRNTASLYDLTPMVKYRIAGADALRFLNRLMTRNIAKLRPGRVAYGVWCN
                     DAGHLIDDGTVFCLGAGEYRLCTAERQLDWLRDSAVGFDVGISEVTEDVAALALQGPT
                     SYSVLRKAGVAGVELLKPFELGEFRLPAAGGAPLMVSRTGFTGDLGYELWMAPVDAER
                     VWDALLEAGRTRGIRPVGSRALNIARIEAGFLLPSVDFVPAERAVFIGTERSPLELGL
                     AWLVDFAKGHFTGRRALLEEQRRGVRRRLVGLDIEGTKPAHNALLYTGRSLRRQAGSI
                     TSAVWSPTTKRNLALAMVEAPHFGTGATLWAEIYLNRELQWERRVMRARVVERPFFAP
                     ERRRATPPGEF"
     gene            1284..>2192
                     /locus_tag="VM713_05915"
     CDS             1284..>2192
                     /locus_tag="VM713_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVH83834.1"
                     /translation="MIATQQKQSADRPWQDPTATPQIVIEGVSKTYGGFTAVASVSLS
                     IYKGEMFALVGASGCGKTTLLRMLAGFAEPTSGRILIDGVPMSGVPPYERPVNMMFQS
                     YALFPHMTVERNVGYGISRMKMDAATRKSRIHEALEMVQLAHVAARKPHQLSGGQRQR
                     VALARALIRRPKVLLLDEPLSALDKKLREQTQFELMDLQWKVGITFIVVTHDQDEAMA
                     LATRIAVMDHGRVVQVGTPAEIYEFPRSRFVADFVGTTNLFEGTVSACDGGLISVRCA
                     ETDGEQLVDDAGSFEPGQRVWISLRPE"
BASE COUNT          340 a          723 c          774 g          355 t
ORIGIN      
        1 gcggagtcat gggaatcgcc gggcgtaacg ccgcgcgcgc ggtcgtgaga gaggcggcct
       61 gagatgctca ccgagagcga acctcactat cgccggccgc tgctgccgac gccgttccac
      121 gaacgcgcgc ggcgcttgag tcaggtcgac agcttcgtac cctgggctgg ctacaccacg
      181 gtcgatgtgt tcacgaccgt cgagcaggag tatttcgcga tccgcaacac cgcctcgctc
      241 tacgatctga cgccgatggt caagtaccgg atcgcgggcg ccgacgccct gcgtttcctc
      301 aaccgcctga tgacacgcaa catcgccaag ctgcgtccgg gccgggtcgc ttacggcgtg
      361 tggtgcaacg acgccggaca tctgatcgac gacggcaccg tattctgtct gggcgcgggc
      421 gagtatcggc tgtgcaccgc cgagcgacag ctcgactggc tgcgcgactc cgcggtcggc
      481 ttcgacgtcg ggatcagcga ggtgaccgag gatgtggccg cgctcgcgct gcagggcccg
      541 acctcgtact cggtgctgcg caaggccgga gttgcgggtg tcgagcttct gaaacccttc
      601 gagctcggcg agttccggct gccggctgcg ggcggcgcgc cgctcatggt gtcgcggacc
      661 ggcttcacgg gggatcttgg ctacgagctg tggatggcgc cggtggatgc cgagcgcgtc
      721 tgggacgcgc tcctcgaggc gggccgcacc cgcgggatcc ggccggtcgg ctcgcgtgcg
      781 ctcaatatcg cgcgcatcga ggccggcttc ctcctgccga gtgttgattt tgttcctgcc
      841 gagcgcgccg tcttcatcgg taccgagcgc tcgccgctcg agctcggcct cgcgtggctg
      901 gtcgattttg ccaaggggca tttcaccggg cggcgggcgt tgctggaaga gcagcgccgg
      961 ggcgtgcggc gccgcctggt ggggctcgac atcgagggca cgaagcccgc tcacaacgcg
     1021 ctgctctaca ccggccggag cctgcgccgc caggcgggca gcatcacctc cgcggtctgg
     1081 tcgccgacca ccaagcgcaa cctcgcgctc gccatggttg aggcgccgca tttcggaact
     1141 ggcgctacgc tgtgggcgga gatctacctc aatcgcgagc tgcagtggga gcgacgagtg
     1201 atgcgcgcgc gggtcgtgga gcgccccttc tttgcgccgg agcggcgccg ggcaactccc
     1261 ccgggggaat tctaggagcg gctgtgatcg ccacccaaca gaagcaaagc gccgatcgac
     1321 cgtggcagga tccgaccgcg acgccgcaga tcgtcatcga gggagtgagc aagacctacg
     1381 gcggcttcac cgcggtcgct agcgtgagtc tcagcatcta caagggcgag atgttcgcac
     1441 tggtaggcgc ttcgggctgc ggcaagacca cgctgctgcg catgctggcg ggattcgccg
     1501 agccgacgag cggccgcatc ctcatcgacg gcgtgccgat gagcggggtg ccgccgtacg
     1561 agcggcccgt caacatgatg ttccagtcgt atgcgctgtt cccgcacatg acggtggagc
     1621 gcaacgtcgg ctacggcatc agccgcatga aaatggatgc ggcgacgcgc aagagccgca
     1681 tccacgaggc gctcgagatg gtgcagctcg cgcacgtcgc ggcgcgcaag ccccaccagc
     1741 tctccggcgg ccagcgtcag cgcgtggcgc tggcgcgtgc gctcatccgg cggccaaagg
     1801 tactgctgct ggacgagccg ctgtcggcgc tcgacaagaa gctgcgcgag cagacgcagt
     1861 tcgagctgat ggacctgcag tggaaggtcg gcatcacgtt catcgtggtg acgcacgacc
     1921 aggacgaggc catggcgctc gccacccgca tcgcggtcat ggatcacggc agggtggtcc
     1981 aggtcggcac gcccgccgag atctacgagt ttccgcgcag ccgcttcgtc gccgacttcg
     2041 tcggcaccac caatctcttc gagggcacgg tcagcgcctg cgacggcggg ctgatcagcg
     2101 ttcgctgcgc cgagaccgat ggggagcagc tggtggatga cgccggcagc ttcgagcccg
     2161 gtcagcgcgt atggatctcg ctgcgcccgg ag
//
LOCUS       DATLKL010000444         3244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_163108, whole genome shotgun sequence.
ACCESSION   DATLKL010000444 DATLKL010000000
VERSION     DATLKL010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163108"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VM713_05920"
     CDS             complement(<1..782)
                     /locus_tag="VM713_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83835.1"
                     /translation="MFAKRSSRALALLLALPSAAFALGLGDIHLNSALNSPLDAEIEI
                     TDVAPDEVSTLRATLAPREVFSQNGIDYPTYLTSVQLRTVETKDGRHIIKLKSTEAIT
                     DPFITLLINVTWVHGQLVREYTMLLDPPVYTPSESAVASTPVAAPAAGADTRAGTITR
                     STEAPAPAATAPAPAAAAEPAAVEPAAAATAQAPAPAEAAAAPPSAPAARSSSKGATA
                     PSAATAAEGASASPAPAGPSAEPSAAQGANTSHVVQKGETLS"
     gene            complement(1106..2209)
                     /gene="aroC"
                     /locus_tag="VM713_05925"
     CDS             complement(1106..2209)
                     /gene="aroC"
                     /locus_tag="VM713_05925"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HVH83836.1"
                     /translation="MSGNTIGKLFTVTTFGESHGPALGCIVDGCPPGLELSEADLQQD
                     VDRRRTGTSHFVSQRREADQVRILSGVFEGRTTGTPIGIVIENTDARSRDYERIKDRF
                     RPGHADYTYQQKYGVRDYRGGGRASARETVMRTAAGAIARRYLATRLGVRIYGFLSQV
                     GPLTLEAVDPAFAYQNPFFCPDPKRVPELEELIWKTREAGDSVGARVTVVASGVPPGL
                     GEPVFDRLDADIAHALMGINAVKAVEIGAGVRSAAQRGSEHRDELTPGGFLSNHAGGV
                     LGGISSGQDILAHLTLKPTSSIQVPGRTIDVEGHAVEIVTTGRHDPCVGLRAVPIAEA
                     MLAIVLMDHYLRQRAQNADVHSPTPVIPGAPRA"
     gene            complement(2214..3128)
                     /gene="prmB"
                     /locus_tag="VM713_05930"
     CDS             complement(2214..3128)
                     /gene="prmB"
                     /locus_tag="VM713_05930"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:TIGR03533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HVH83837.1"
                     /translation="MPRRLKRPRRKPSPPFTVGRLIARGARRLKGARVHFGHGTDNAW
                     DEAASLTLHALGLGQGGGAALYGRRVNRADAARAGELIGRRIAERVPAPYLTDVAWFT
                     VLEFAIDARVLVPRSPLAELIERGFAPWADVRRVRRILDLGTGSGCIAIAAAKALPTA
                     RVDAVDISAAALEVARLNVRRHRLARRVRLRRSDHFRALRKATYDIIVANPPYVGARE
                     LKRLPPEYRHEPRLALAAGARGLDSVAVILREARRHLRKKGLLIVEVGNTEHAVRRAY
                     PHLPFLWLEFARGGGGVFLLTREQLVAH"
BASE COUNT          473 a         1080 c         1180 g          511 t
ORIGIN      
        1 tcggagagcg tctcaccctt ttgcacgacg tgactcgtgt tggcgccctg cgccgcgctc
       61 ggctcggccg acgggccagc cggggcgggg ctcgcgctcg cgccctccgc agccgtcgcc
      121 gcgctcggcg ccgttgcgcc cttgctggag ctgcgcgcgg caggcgcgct cggtggcgca
      181 gcggcagcct cggcgggcgc cggcgcctgc gcagtcgcgg ccgccgcggg ctcaacggcg
      241 gcaggctcgg cggctgcggc cggtgcggga gctgtggccg cgggagccgg agcttcggtg
      301 ctgcgtgtga tcgtccccgc gcgcgtgtcg gcgcccgccg ctggcgctgc caccggggtg
      361 ctcgccaccg ccgactcgct cggcgtgtac accggcggat cgagcagcat ggtgtactcg
      421 cgtaccaact gcccgtgcac ccaggtgacg ttgatcagga gtgtgatgaa gggatcggtg
      481 atcgcctcag tcgacttgag cttgatgatg tgccggccgt ccttggtctc gaccgtgcga
      541 agctgaacgc tcgtcaggta cgtggggtag tcgatgccgt tctgcgagaa gacttcgcga
      601 ggcgccagag tcgcccgcag cgtgctgacc tcatcggggg cgacgtcggt gatctcaatc
      661 tccgcatcga ggggagagtt cagtgccgag ttgagatgaa tgtctccgag accaagggca
      721 aacgccgctg acggcaaagc cagcagcaat gccagcgccc ggcttgagcg tttggcgaac
      781 atcagggcac cccggcagga gtcatttgaa atatcttgta gaaacaatcg gatggacgat
      841 aagttgcgag tccatcacga attgggccgc aatataactt aaataggctt gcaacccaaa
      901 gccttagggc ttccacggcc gtcgatcgcg gctgttcggc gcacattgtc gggtacagtc
      961 ctcgagtgaa atccccgctc agaagcagaa tttttacgga ttcttaggac ataaactgtg
     1021 tcgcggttcg cctcggaggc ggatcgcggg ccacaggccg cggtggaact gtgaacgccg
     1081 ccacatcttt tcggtgcgcc gctcgtcagg cgcgcggggc ccctggaatt accggcgttg
     1141 gcgagtgcac atcggcgttc tgcgcccgct ggcgcaggta gtggtccatc agcacgatcg
     1201 cgagcatcgc ctccgcgatc ggcacggcac gcagccccac gcacgggtcg tgccgtccgg
     1261 tcgtgacgat ctcgaccgca tggccctcga cgtcgatggt ccgccccggc acctggatac
     1321 tggaggtcgg tttgagcgtc agatgcgcga ggatgtcctg gccggaggag atgccgccga
     1381 ggacgccgcc ggcgtgattg ctgaggaacc cgcccggcgt cagctcgtcg cggtgctcgc
     1441 tgccgcgctg cgccgccgag cggacgccgg cgccgatctc gaccgccttc acggcgttga
     1501 tgcccatgag cgcatgggcg atgtccgcat ccagccggtc gaacaccggc tcgcccaagc
     1561 ccggcggcac gccgctcgcg accacggtga cgcgcgcgcc gaccgagtcg cccgcttcgc
     1621 gcgtcttcca gatgagctcc tcgagctcgg gcacgcgctt cgggtctggg cagaagaacg
     1681 gattctggta ggcgaaggcg ggatccaccg cctcgagggt gagtgggccg acctggctca
     1741 ggaagccgta gatgcgtacg ccgagccgcg tggcgagata gcgccgcgcg atcgcgccgg
     1801 ccgcggtgcg catcaccgtc tcgcgcgcgc tcgcgcggcc gccgccgcgg tagtcgcgca
     1861 cgccgtactt ctgctgatag gtgtagtcgg catgccccgg gcggaagcgg tccttgatgc
     1921 gctcgtagtc gcgcgagcgc gcgtcggtgt tctcgatgac gatgccgatc ggcgtgccgg
     1981 tcgtgcgccc ctcgaagact ccggagagga tgcgtacctg gtcggcctcg cgacgctggc
     2041 tcacgaagtg cgacgtaccg gtgcggcgcc ggtcgacgtc ctgctgcaga tccgcttcgg
     2101 agagctcgag ccccggcggg cagccgtcga cgatgcatcc gagcgccggc ccgtggcttt
     2161 cgccgaaggt ggtgacggtg aagagcttgc cgatggtgtt gccggacatg gcgctagtgt
     2221 gccaccagtt gctcgcgtgt cagcaggaac accccgccgc cgccgcgcgc aaactcgagc
     2281 cacaggaacg gcaggtgcgg gtaggcgcgc cgcaccgcgt gctcggtgtt gccaacctcg
     2341 acgatgagga gtcccttctt gcgcaggtgg cggcgcgcct cgcgcaggat gacggccacc
     2401 gagtcgaggc cacgcgcacc ggctgcgagc gcgaggcgcg gctcgtgccg gtattccggc
     2461 ggcagtcgtt tgagctcacg ggcccctacg taaggcggat tggcgacgat gatatcgtag
     2521 gtggccttgc ggagcgcgcg gaagtggtcg gagcgcctca ggcgcacgcg ccgcgcgagg
     2581 cgatggcgcc gcacgttgag gcgtgcgact tcgagcgccg cggcggagat gtccacggcg
     2641 tcgacgcggg cggtcggcaa cgccttcgcg gcggcgatgg cgatgcagcc ggagcccgtg
     2701 ccgagatcga ggatgcgccg cacgcgccgc acgtcggccc acggagcgaa gccccgctcg
     2761 atgagctctg cgagcggcga gcgcggcacg agcacgcgcg catcgatcgc gaactcgagg
     2821 accgtgaacc aggcgacgtc ggtgaggtag ggggccggca cgcgctcggc gatgcgccgc
     2881 ccgatgagct ctccggcacg cgccgcgtcg gcccgattca cgcgccgccc gtagagagcg
     2941 gcgccgccgc cttggccgag cccgagcgcg tgcagcgtaa ggctcgcggc ctcatcccat
     3001 gcattgtcgg tgccgtggcc gaaatggacg cgggcgccct tgaggcgccg tgcgccgcgc
     3061 gcgatgaggc ggcccacggt gaagggcggg ctcggcttac gccgcgggcg cttcagccgg
     3121 cgtggcatcc accctcgccg cggccccggc cgcgcgccgg ggtatgaaat aattccctca
     3181 tgggcaccgt gaatgccgga gcgccgtgga ccgccgtgcg caggccttgg ttttggatcc
     3241 tcgt
//
LOCUS       DATLKL010000445         1862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_118144, whole genome shotgun sequence.
ACCESSION   DATLKL010000445 DATLKL010000000
VERSION     DATLKL010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118144"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="VM713_05935"
     CDS             complement(<1..1165)
                     /locus_tag="VM713_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVH83838.1"
                     /translation="MKMRMVTIATAVVVLSAAASGQALAADEVVIGDIDDMSGVYADV
                     MGAAGVEAVKMAIADFGGTVLGRKITLLTADHQNKPDIGAAKFREWADQNGLNLLLGG
                     SNSGVNIAMSKVAAVKKVPFFTIGAAGASLTNEDCTAYSVHYVYDTTSLANGTATAIV
                     AAGGKTWFYLTADYAFGTQLQNAASKVVEAHGGKNLGAVRVPLGAADFSSFLLQAQAS
                     GAQVLGLSNAGTDFSNSLKAANEFGITKTMKPAALLAFITDINSLGLPTAQGLYLTTG
                     FYWDLSPETRAFGKRFYTKINRMPTMDQAGAYSATLTYLKALKAAGTTDSDKVMAELK
                     KTRINDVFDKDGYIRADGVMVHEMYVMQVKAPQESKYPWDYYKVVRRMPGEEAF"
     gene            complement(1194..>1862)
                     /locus_tag="VM713_05940"
     CDS             complement(1194..>1862)
                     /locus_tag="VM713_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVH83839.1"
                     /translation="AYYGESHVLHGVDCHVGRGEVVTLLGRNGAGRTTTLRSLLGLVG
                     ARSGSVMINGAETIGLPPHRIARLGIGYCPEERGIYASLSTEENLELPPPVAPGGMSV
                     EEIYAMFPNLKERRASRGSHLSGGEQQMLALARILRTGARLLLLDEISEGLAPVIVQA
                     LGRTIQKLRVQGYTMVMSEQNFRFAAPLADRFYIMERGRIVAGFAAHELQEKMPLLHQ
                     YLGV"
BASE COUNT          308 a          596 c          619 g          339 t
ORIGIN      
        1 cgaaggcctc ctcgccgggc atccgccgca ccaccttgta gtaatcccag ggatacttcg
       61 attcctgcgg cgccttcacc tgcatcacgt acatctcgtg gaccatgacg ccgtccgcac
      121 ggatgtagcc atccttgtcg aacacgtcgt tgatcctggt tttcttgagc tccgccatga
      181 ctttgtcgga gtcggtggta ccggccgcct tcagggcctt gaggtaggtc agggtggccg
      241 aatacgcgcc cgcctggtcc atggtcggca tgcggttgat cttggtgtag aaacgcttgc
      301 cgaaggcgcg cgtctccggg ctcaggtccc aatagaagcc cgtggtgagg tacagcccct
      361 gcgcggtcgg gaggcccaga ctgttgatat cggtgatgaa ggcgagcagc gctgccggct
      421 tcatggtctt ggtgatgccg aactcgttgg cggccttcag ggagttactg aagtcggtgc
      481 cggcgttgga gagcccgagc acctgtgcac ccgaggcctg ggcctgcagc aggaatgagg
      541 agaagtccgc cgcgcccagc ggcacgcgca ccgcgccgag gttcttgccg ccgtgcgcct
      601 cgaccacctt ggaggcggca ttctgcagct gggtgccgaa ggcatagtcg gcagtcagat
      661 agaaccaggt cttgccgccc gcggcgacta tggcggtcgc ggtaccgttg gcgagcgagg
      721 tcgtatcgta gacatagtgg acggagtagg cggtgcagtc ctcgttggtg agagaggcac
      781 cggccgcgcc gatggtgaag aacgggacct tctttacggc ggcgaccttc gacatggcga
      841 tgttgacacc actgttggag ccgcccagca ggaggttaag accgttctgg tcggcccatt
      901 cgcggaactt ggccgcgccg atgtcgggct tgttctggtg gtcggcggtc agcagcgtga
      961 tcttcctgcc gagcaccgta ccgccgaagt cggcgatcgc catcttgacg gcttcgacgc
     1021 cggcggcacc catgacgtcc gcatagacgc ccgacatgtc gtcgatgtcg ccgatcacca
     1081 cctcgtcagc cgccagcgcc tgcccgctgg ccgcagcgct gagcactact accgccgtcg
     1141 caatggtcac cattcgcatt ttcatgagtc gtgctcctcg agttggtgcg ggctcagacg
     1201 cccaggtact ggtgcagcag gggcattttc tcctgaagct cgtgcgccgc aaaacccgcg
     1261 acgatccgcc cgcgctccat gatgtagaag cggtccgcca gcggagccgc gaagcggaaa
     1321 ttctgctccg acatgaccat ggtgtagccc tgcacgcgca gcttctggat ggttctgccg
     1381 agtgcctgca cgatgacggg cgcgaggccc tcggaaatct catcgagcag cagcagacgc
     1441 gcgccggtgc gcaggatccg cgccagcgcc agcatctgct gctcgccgcc ggaaaggtgc
     1501 gatccccggc tcgcgcgccg ctccttcaag ttgggaaaca tagcgtagat ctcttcgact
     1561 gacattcccc cgggcgcgac cggcgggggg agctcgaggt tttcctcggt cgagaggctg
     1621 gcatagatac cgcgctcctc cgggcagtag ccgatgccga gccgggcaat gcggtggggc
     1681 ggcaggccga tggtctccgc gccgttgatc atcaccgagc cggagcgcgc gcccacgagc
     1741 cccagaagcg aacgcagcgt ggtggtgcgg ccggcgccgt tgcggccgag gagcgtcacg
     1801 acttcgccac gccccacgtg gcagtcgacg ccgtgcagca catgcgactc gccgtaatag
     1861 gc
//
LOCUS       DATLKL010000446         1790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_95551, whole genome shotgun sequence.
ACCESSION   DATLKL010000446 DATLKL010000000
VERSION     DATLKL010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95551"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /gene="speB"
                     /locus_tag="VM713_05945"
     CDS             <1..933
                     /gene="speB"
                     /locus_tag="VM713_05945"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HVH83840.1"
                     /translation="MKEPRFAEIATFMRCPLAATLDNVDIGLIGVPTDIAVTNRPGAR
                     HGPREIRNSSSLMRGFNVALGVNPYELCRVADLGDVPISHRYDLERQNDAIEAFYRRV
                     KAAGILPVSAGGDHSITYPIFRALGAERPVGMVHVDAHCDTWGEFFGSKFTHGAPFRL
                     AVEAGVLDPKRTIQIGIRGAQNFLDGIEFSKSHGMRVVFMDEFAELGVDKVIQLARKV
                     VGDGPTYISFDVDGLDPVYAPGTGTPEIGGITTLEAQRLLRGLRGLDLIGGDVVEVAP
                     PYDQTGNTALVGATMMYEILCLIADRHFGAARRA"
     gene            930..>1790
                     /locus_tag="VM713_05950"
     CDS             930..>1790
                     /locus_tag="VM713_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH83841.1"
                     /translation="MSGEPRGSLEVALQHATRLLASQPTLAAEQASEILKVVPGQPAA
                     LLLLAVAQRACGDLPAALQGLEELSRRQPAWAEAQFELGMTLGAAGQGESAIAALRRA
                     VSLKPDLPDAWRALADHLAGAGDTAGAAEAYARHIKAATRDPRLLAPAAALVENRIPE
                     AEALLRAHLRAHPTDVAALRMLAEVAARLRRWRDSQTLLERCLELAPGFDAARHNYAL
                     VLNRQGKAAAALPHVEQLLAKEPRNPGYRNLRAAVLVNLGEYAESARIYAGVLAEFPQ
                     QPKVWMSYGHS"
BASE COUNT          267 a          662 c          594 g          267 t
ORIGIN      
        1 atgaaggagc cgcgctttgc cgaaatcgcc accttcatgc gctgccccct cgcggccacc
       61 ctcgacaacg tcgacattgg cctcatcggc gttccgaccg acatcgcggt caccaaccgc
      121 ccgggcgcgc gccacggccc gcgcgagatc cgtaattcct cgagcctgat gcgcggcttc
      181 aacgtcgcgc tcggcgtcaa cccctacgag ctctgccgcg tcgccgacct gggcgacgtg
      241 ccgatctccc accgctacga cctcgagcgg cagaacgacg ccatcgaggc gttctatcgc
      301 cgcgtcaagg cggccggcat cctgccagtg agcgccggcg gcgatcactc catcacctac
      361 ccgatcttca gggcgctcgg cgccgagcga ccggtcggca tggtgcacgt cgatgcgcac
      421 tgcgatacct ggggcgagtt cttcggctcg aaattcacgc atggcgcacc atttcgcctc
      481 gcggtcgagg cgggcgtgct cgacccgaag cgcaccatcc agatcggtat ccgcggcgcg
      541 cagaacttcc tcgacggcat cgagttctcg aagtctcacg gcatgcgcgt cgtcttcatg
      601 gacgagttcg ccgagctcgg cgtcgataag gtgatccagc tggcgcgcaa ggtcgttggc
      661 gacggcccaa cctatatctc cttcgacgtc gacggactgg accccgtgta tgcgcccggc
      721 accggcacac ctgagatcgg gggcatcacg acactcgagg cgcagcgcct gctgcggggc
      781 ctcaggggtc tcgacctgat cggtggcgac gtcgtcgaag tcgcgccgcc ctacgaccag
      841 accggcaaca ccgcgctcgt cggtgccacg atgatgtacg agatcctctg tctgatcgcc
      901 gaccggcact tcggcgccgc gcggcgggcg tgagcggcga gccacgaggc tcgctcgagg
      961 tggcgctgca gcacgccacg cgcctcctcg cctctcaacc gacgctcgcc gcggagcagg
     1021 ccagcgagat cctcaaggtc gtgcctgggc aacccgctgc gctgctgctg ctcgccgtgg
     1081 cgcagcgcgc ctgcggtgat ctgcccgcgg cgttgcaagg gctcgaggaa ctcagccggc
     1141 ggcaaccggc gtgggccgaa gcacagttcg agctcggcat gacgctcggg gccgccggcc
     1201 agggtgagtc ggcgattgcg gcgctgcgcc gtgccgtctc gctcaaaccc gacctgccgg
     1261 acgcctggcg cgcgctcgcc gaccacctcg cgggcgcggg cgacacggcc ggcgccgccg
     1321 aggcctacgc ccggcacatc aaggccgcga cgcgtgatcc gcgtctgctc gccccggcgg
     1381 ccgcactggt tgagaaccgc attcccgagg ccgaagcgct gctgcgcgcg cacctgcggg
     1441 cccacccgac cgacgtcgca gctctgcgca tgctcgcgga ggtggcggcg cggctgcggc
     1501 gctggcgcga ctcgcagacc ttgctcgagc gctgcctcga gctcgcgccc ggcttcgatg
     1561 ccgcgcgtca caactacgcc ctggtgctca accgtcaggg caaggcggcg gcggcgctcc
     1621 cgcacgtcga gcagctgctc gccaaggagc cacgcaatcc cggctacaga aatctccggg
     1681 ccgcggtgct cgtcaacctc ggtgagtacg ccgagtcggc gcgcatctac gccggggtgc
     1741 tggcggagtt cccgcagcag ccgaaggtgt ggatgagcta cgggcactct
//
LOCUS       DATLKL010000447         4568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_106739, whole genome shotgun sequence.
ACCESSION   DATLKL010000447 DATLKL010000000
VERSION     DATLKL010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106739"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="VM713_05955"
     CDS             <1..1270
                     /locus_tag="VM713_05955"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HVH83842.1"
                     /translation="KAELLSTLNNFLIEQHAAGRKVLLIVDEAQNLEARVLEEIRLLS
                     GVETTKEKVLRIILAGQPELNETLNAPRLEQLAQRVRLRFHLTALSESETRAYIEHRL
                     EVAGASDREIFVPDTFHEIFRYTGGVPRLINTLCDTAMMAAFTENRDMVRLADVASAV
                     AELRWPLYPERVQAAAARAQAAAAARAQAAAAAAPENETESDEEVPEAPAVGTPSPES
                     AARETRARPTGGHGAAAHEAAAREAAAQEAVADDAVAKAVAQTVARETAAREGAAKTM
                     AAATEPVGKLLVATDGRTVQEVPLRIGRLIIGRTPDNDIHIDSRFVSRHHCQVITTAH
                     SCVIEDLNSTNGIYVKSARVRRHYLNDGDVVLIGKHELIYVDERAARGRPTASETEPK
                     LQAVSEVPELRGTPAAEGSGDGKSKRQVGG"
     gene            complement(1321..1569)
                     /locus_tag="VM713_05960"
     CDS             complement(1321..1569)
                     /locus_tag="VM713_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83843.1"
                     /translation="MEHRHLSRPVYEGLPWFYMAFGVAALGASYGLAAQKALSHGLGL
                     LGLIVLVYGIVVFLRRRDYRALRSEYGNPDSLSDDNPR"
     gene            complement(1654..1821)
                     /locus_tag="VM713_05965"
     CDS             complement(1654..1821)
                     /locus_tag="VM713_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83844.1"
                     /translation="MQAEPNLSMSDTSTLKLPAELLAELKRHTKPKNDFRVESFKPSL
                     LERLLGMFGLK"
     gene            complement(2097..3584)
                     /gene="tldD"
                     /locus_tag="VM713_05970"
     CDS             complement(2097..3584)
                     /gene="tldD"
                     /locus_tag="VM713_05970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HVH83845.1"
                     /translation="MSAPDSRAPVPAAPPAAADPLSMARELILRPGGLDDRRLDKVFG
                     EVLRHSVDFADLYFQLAHQESWTLEDGIVKDGSASIEQGVGVRALAGEKTGFAYSDEI
                     VLPALEEASRAARAIAVQGGERRVSAWHAAHGHQLYLPADPAAALAAADKVAWLERAD
                     REARRLDPRVVHVNASLVAVQEVVLIATSEGELAADVRPLVRFNVSVLVEQNGRREQG
                     YAGAGGRYSLAELVASDKPLELAREAVRQALINLEAVPAPAGTMTVVLGPGWPGILLH
                     EAIGHGLEGDFNRKGTSAFANRIGERVAAAECTVVDDGTLARRRGSLNIDDEGTPTQC
                     TTLIENGVLRGYIQDRLNARLMGVAPTGNGRRESFAHTTLPRMTNTYMRPGPHDPGEI
                     IRSVARGIYAVNFGGGQVDITSGKFVFSASEAYAIENGRITAPLKGATLIGNGPDVLT
                     RVTMVGNDLKLDEGVGTCGKEGQSVPVGVGQPTLRIEALTVGGTA"
     gene            complement(3581..4405)
                     /locus_tag="VM713_05975"
     CDS             complement(3581..4405)
                     /locus_tag="VM713_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HVH83846.1"
                     /translation="MRVAALQMTSGPEVEANLANAAALLEEAASRGARLAVLPENFAF
                     LGLRDADKRAVAEADGAGPVQQFLAESSRRLKLWLVGGTIPLKSGADGRVAAASLVYD
                     AGGARVARYDKIHLFDVDIPGRAETYRESANVAPGTRAVVLDTPAGRLGLSVCYDVRF
                     PELYRALSAAGAQLLSVPSAFTAPTGRAHWETLLRARAIENLCYVIAPAQSGIHPSGR
                     ETYGDSMIVDYWGRILKRVPRGRGCAVAEVDLARQSAVRESFPALQHRTFIGEAHP"
BASE COUNT          709 a         1604 c         1592 g          663 t
ORIGIN      
        1 caaggcggag ctgctcagca ccctcaacaa cttcctcatc gagcagcatg ccgccggacg
       61 caaggtgctg ctcatcgtcg acgaggcaca gaatctcgag gcgcgcgtgc tcgaggagat
      121 ccgcctgctg tcgggcgtcg aaaccaccaa ggagaaggtg ctgcgcatca ttctcgccgg
      181 gcagcccgag ctcaacgaga cgctcaatgc cccgcggctc gagcagctcg cccaacgggt
      241 gcggctgcgc ttccatctca cggcgctgtc ggaatccgag acgcgcgcct atatcgagca
      301 ccggctggag gtggccgggg cgagcgaccg cgagatcttc gtgcccgaca ccttccacga
      361 gatcttccgc tataccggcg gggtaccgcg gctcatcaac acgctgtgcg acaccgcgat
      421 gatggcggca ttcacggaga accgcgacat ggtgcgcctc gccgatgtcg cgagcgccgt
      481 ggcggagctg cgctggccgc tgtatccgga gcgtgtgcag gcggcggcgg cgcgcgctca
      541 ggctgcggca gcggcgcgcg cccaggcggc cgcggcagct gcgccggaga acgagaccga
      601 gagcgacgag gaggtgccgg aggcgccggc ggtgggcact ccctcgcccg agtccgccgc
      661 gcgcgagacg cgagcgcgcc cgacgggtgg gcacggggcg gccgctcacg aggcagccgc
      721 tcgcgaggca gccgctcagg aggcggtcgc tgacgatgcg gtcgctaagg cggtcgctca
      781 gacggtcgcc cgggagacgg ccgcgcgcga gggcgccgcg aagacaatgg ccgcagcgac
      841 cgagccggtc gggaagctgc tcgtggcgac cgacgggcgc accgtgcagg aggtccccct
      901 gcgcatcggc cggctcatca tcgggcgcac tcctgacaac gacatccaca tcgacagccg
      961 cttcgtgagc cgccatcact gccaggtcat caccacggcg cactcgtgcg tgatcgagga
     1021 tctcaacagc accaacggca tctatgtcaa atcggcacgc gtgcggcgcc actacctcaa
     1081 cgacggcgac gtcgtgctga tcggcaagca cgagctgatc tatgtggacg aacgcgcggc
     1141 gcggggccgc ccgaccgcat ccgagaccga gccgaagctc caggcggtga gcgaggtgcc
     1201 ggagctgcgc gggacgccag ccgcagaggg gtctggagac ggcaagagca agcgtcaggt
     1261 gggcggctga ggcccccggg ccccgcccga tggcggcgag ttaagccgct ctacgcggcc
     1321 tcacctgggg ttgtcatcgc tgagcgaatc gggattgccg tactcactgc gcagcgcgcg
     1381 gtagtcgcgg cggcgcagga acacgacgat tccgtacact aacacgatga ggccgaggag
     1441 cccgaggccg tggctcagcg ccttttgcgc cgccagcccg tagctcgcgc cgagcgccgc
     1501 gaccccgaag gccatgtaga accagggcag gccctcgtag acgggacggg aaagatggcg
     1561 gtgctccatg ccggcatcct aacttgccgg cgcggcggcg gatactcgcc ccctgaaatt
     1621 gccccccgca ggggatgcgg cagcgtgctg cggctatttc aggccgaaca tccccagcag
     1681 gcgctcgagg agagacggct tgaagctctc gacccgaaaa tcgttcttcg gcttggtatg
     1741 acgcttgagc tccgccagca gctccgcggg gagcttcagg gtggaggtgt cggacatcga
     1801 caagttgggc tcggcttgca tcgtgtcgtc cttcctggcg gcagtgtctg cgggatgcac
     1861 gctacggact cggctcgttc gggggcgtgt ggagattcac aaaccagcca acgctcccga
     1921 ttatgtagtg gttgcggtgc gggccgcgcc ttgcgtctgc acgcggagcg atcctggccg
     1981 gcccgcggct gcaagcatcg cgctgcgccg cgtgcctact cacggcacat attggcgtgc
     2041 tgcgtccaga atgcgaaatg tcgtgtaacg acgacgtcag tagacgccga cagctctcag
     2101 gcggtccccc cgaccgtgag cgcctcgatc ctgagcgtcg gctgacccac gccgaccggc
     2161 acgctctgcc cttctttgcc gcaggtgccg acaccctcgt cgagcttcag atcgtttccc
     2221 accatcgtga cgcgcgtcag cacgtcgggc ccgttgccga tcagcgtcgc gcccttgagc
     2281 ggagctgtga tgcgtccgtt ctcgatggcg taggcctcgc tcgccgagaa cacgaacttt
     2341 cccgaggtga tgtccacctg tccgccgccg aaattgacgg cgtagatgcc gcgggccacc
     2401 gagcggatga tctcgcccgg gtcgtgagga ccggggcgca tataggtgtt ggtcatgcgc
     2461 gggagcgtcg tgtgggcaaa ggactcgcgc cggccgttgc cggtgggtgc gactcccatg
     2521 agacgcgcgt tcaggcgatc ctggatgtag ccgcgcagca cgccattctc gatgagcgtc
     2581 gtgcactgcg tcggcgtgcc ctcgtcgtcg atattgagcg agccgcggcg gcgcgccagc
     2641 gtgccgtcgt ccaccaccgt gcactccgcc gccgccaccc gctcgccgat gcggttggcg
     2701 aacgccgagg tgcccttgcg gttgaagtcc ccctcgagcc cgtggccgat ggcctcgtgc
     2761 agcaggatgc cgggccaccc ggggcctagc accaccgtca tggtgccggc gggcgccggc
     2821 acggcctcga ggtttatcag cgcctgccgt acggcctcac gcgcgagctc gagcggcttg
     2881 tcgctcgcga ccagctcggc gagcgagtag cgcccgcccg ccccggcgta accctgctcg
     2941 cgccggccgt tctgctcgac cagcaccgag acgttgaagc gcaccagcgg gcgcacgtcg
     3001 gccgcgagct cgccctcgct ggtggcgatg agcaccacct cttggaccgc gaccaggctc
     3061 gcgttgacgt gcacaacgcg cggatcgagc cgccgcgcct cgcgatcggc gcgctcgagc
     3121 caggccacct tgtcggccgc ggcgagcgcg gcagccgggt ccgccggcag gtacagctgg
     3181 tggccgtggg cggcgtgcca ggcgctcacg cgccgctcgc cgccctgcac ggcgatggcg
     3241 cgcgccgcgc gcgaggcctc ctcgagcgcc ggcaggacga tctcgtcgga gtacgcgaag
     3301 ccggtcttct cgccggcgag cgcgcgcacg ccgactccct gttcgatgct ggcgctgccg
     3361 tccttgacga tgccgtcctc gagcgtccag gactcctggt gcgcgagctg gaaatacaga
     3421 tccgcgaagt cgaccgagtg ccgcagtacc tcgccgaaga ccttgtcgag gcggcggtcg
     3481 tcgagcccgc cggggcgcag gatcagctcg cgtgccatgc tgagtggatc ggccgccgcc
     3541 ggcggcgcgg ccggcaccgg agcacgcgaa tcgggtgcgc tcacgggtgc gcctccccga
     3601 tgaaggtgcg gtgctgcagg gccggaaagc tctcgcgcac ggcgctctgg cgcgcgaggt
     3661 ccacctcggc cacggcgcag ccgcggccgc gcggcacgcg cttgaggatc ctgccccagt
     3721 aatcgacgat catgctgtct ccataggtct cgcgcccgct cggatgaatc ccggactgcg
     3781 ccggtgcgat cacgtagcag aggttctcga tcgcacgggc gcgcagcagt gtctcccagt
     3841 gcgcgcgccc ggtcggcgcg gtgaaagccg acggcacgga gagcagctgc gcccccgcgg
     3901 cgctcagggc ccggtacaac tccggaaacc tcacgtcgta gcataccgat agtccgaggc
     3961 gtcccgccgg cgtatcgaga accaccgccc gcgtccccgg agcgacgttg gccgactcgc
     4021 gataggtttc ggcgcggccg ggaatatcga cgtcgaacag atggatcttg tcgtagcgcg
     4081 cgacgcgggc accgccggca tcgtagacga gcgaggcggc ggccacgcgc ccgtcggcgc
     4141 cgctcttcag gggaatggtg cctcccacga gccagagctt gagccgtcgc gagctctcgg
     4201 caagaaactg ctgcaccggg ccggcgccgt cggcttccgc caccgcgcgc ttgtcggcgt
     4261 ccctcaagcc gaggaacgcg aagttctccg gcagcaccgc gaggcgcgca cctctcgagg
     4321 cggcttcctc gagcagcgcc gctgcattgg cgagattcgc ctcgacctcc ggtcccgagg
     4381 tcatctgcag ggccgcgact ctcatgggct aaccatgggg tcatcccacg tgccgtgcaa
     4441 gcgcagcgcg gcgcgctcgt ggctggagcc ggcaagcagc tcccgcagcg acatccagag
     4501 cgccgccacc cgtggtccgg gactcaagcg ccgcacggcg gccggcagcc gatcctcgcc
     4561 cttcagca
//
LOCUS       DATLKL010000448         2589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_73199, whole genome shotgun sequence.
ACCESSION   DATLKL010000448 DATLKL010000000
VERSION     DATLKL010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73199"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2151)
                     /gene="clpA"
                     /locus_tag="VM713_05980"
     CDS             complement(<1..2151)
                     /gene="clpA"
                     /locus_tag="VM713_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HVH83847.1"
                     /translation="MLSNELEYCLNDAFHQAREARHEYLTVEHLLLAILDTPKVREVL
                     RACGVDFAKLRQELKEHIDQSTPRLEDGEEREVQPTLGFQRVLQRALFHVQSSGKKEV
                     GVTNVLVAIFSEKQSHAVFLLNRQHVTRLDVVNYISHGLSKIAEEKSDKEEASAEGER
                     DGEGGSALEKYTTNLNRMAQDGRIDPLIGRKLEVERTIEILCRRRKNNPLYVGEAGVG
                     KTAIAEGLARLIVEGKVPEVLADCTIFALDMGALIAGTKYRGDFEKRLKSVITEMKKL
                     PGAVLFIDEIHTVIGAGAASGGVMDASNLIEPVLSNGEIRCIGSTTYSEYRGIFEKDH
                     ALARRFQKIDVVEPSVAETIDILFGLKPRFEEHHGITYADEALRAAAELAARHINERH
                     LLDKAIDVVDEAGARARLKPVAEREPVVQVRHIEDVVARMARIPAKSVSISDKEVLKN
                     LERNLKLVIFGQDKAIESLGAAIKMARSGLGEARKPVGSFLFAGPTGVGKTEVTRQLA
                     IAMGVEFLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAITKHPHCVLLLDEVE
                     KAHPDVFNLLLQVMDHGTLTDNNGRKADFRHVIIVMTTNAGAQEMARPSIGFTHSDNA
                     SDGMEAIRRIFTPEFRNRLDAVIQFASLDPVTIERVVDKLVLEVESQLEQKGVTITLD
                     DAARRWIAEKGYDPKMGARPMARTIQEHIKRPLAE"
     gene            complement(2208..2498)
                     /gene="clpS"
                     /locus_tag="VM713_05985"
                     /pseudo
     CDS             complement(2208..2498)
                     /gene="clpS"
                     /locus_tag="VM713_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
BASE COUNT          407 a          835 c          830 g          517 t
ORIGIN      
        1 ctcggcgagt ggccgcttga tgtgctcctg gatcgtccgt gccatcggac gcgcgcccat
       61 cttcgggtcg taacctttct cggcgatcca gcgccgcgca gcgtcatcga gtgtgatggt
      121 cacgcccttc tgctcgagct gcgactccac ctccagcacc agcttgtcga ccacgcgctc
      181 gatggtgacc gggtcgaggg acgcgaactg gatgacggcg tcgaggcggt tgcggaactc
      241 gggcgtgaag atgcgccgga tcgcctccat gccgtcgctc gcgttgtcgg agtgcgtgaa
      301 gccgatcgag ggacgcgcca tctcctgagc gccggcattg gtcgtcatga cgatgatgac
      361 gtggcggaag tcggcttttc tgccgttgtt gtccgtcagc gtgccgtgat ccatcacctg
      421 cagcagcaga ttgaacacgt ccggatgcgc cttctcgacc tcatcgagca gcagcacgca
      481 gtgcgggtgc ttggttatgg cctcggtgag gagcccgccc tggtcgaagc cgacgtagcc
      541 gggcggcgcg ccgatcaggc gcgaaaccgt gtgcctctcc atgtactcgg acatgtcgaa
      601 gcgcaggaac tcgaccccca tggcgatggc gagctgccgc gtaacttcgg tcttgccgac
      661 tccggtggga ccggcgaaca ggaagctgcc gacgggcttg cgcgcctcgc cgaggcccga
      721 gcgcgccatc ttgatcgcag ctcccaggga ctcgatcgcc ttgtcctggc cgaagatcac
      781 gagcttcagg ttccgctcga ggttcttcag gacctccttg tcagagatcg agacgctctt
      841 ggcgggaatg cgcgccatgc gcgccaccac atcctcgatg tgccgcacct gcaccaccgg
      901 ctcgcgctcg gctaccggct tcaaacgcgc gcgcgcgccg gcctcgtcga ccacgtcgat
      961 ggccttgtcg agcagatgtc gttcgttgat gtggcgtgcg gccagctccg ccgcggcgcg
     1021 cagcgcctca tcggcgtaag tgatgccgtg atgctcttcg aagcgcggct tcagcccgaa
     1081 caggatgtcg atggtctcgg ccaccgaggg ctcgactacg tcgatcttct ggaagcgccg
     1141 ggccagggcg tggtccttct cgaagatccc gcggtactcg ctgtaggtgg tcgagccgat
     1201 gcagcggatc tcaccgttgg agagcaccgg ctcgatgaga ttggaggcgt ccatcacgcc
     1261 gcccgaggcg gcgccggcgc cgatcacggt atggatctcg tcgatgaaga gtaccgcccc
     1321 ggggagcttc ttcatctcgg tgatcacgct cttcaggcgc ttctcgaaat cgccgcggta
     1381 tttggtgccg gcgatcagcg cgcccatgtc gagcgcaaag atggtgcagt cggcgagcac
     1441 ctcgggcacc ttgccctcga cgatgagccg cgcgagtccc tcggcgatcg cagtcttgcc
     1501 gacgccggcc tcgccgacgt acagcgggtt gttcttgcgg cggcggcaca ggatctcgat
     1561 cgtgcgctcg acctcgagct tgcgtccgat cagcgggtcg atgcgcccgt cctgcgccat
     1621 gcggttgaga ttggtggtgt atttctcgag cgcgctgccg ccctcgccat cgcgctcgcc
     1681 ctcggctgag gcctcctcct tgtcgctctt ctcctctgcg atcttcgaga gcccgtggga
     1741 gatgtagttg acgacgtcga gccgcgtgac gtgctggcga ttcagcagga acacggcatg
     1801 gctctgcttc tcactgaaga tggcgaccag tacgttggtc accccgacct ctttcttgcc
     1861 gctcgactgc acgtggaaca gcgctcgctg cagcacacgc tggaagccga gggtcggctg
     1921 cacctcgcgc tcctcgccgt cctcgagacg cggcgtggac tgatcgatgt gctccttgag
     1981 ctcctggcgg agcttggcga aatccacccc gcaggcgcgc agcacctcgc gcaccttggg
     2041 ggtgtcgagt atggcgagga gcagatgctc gaccgtcagg tactcgtgac gcgcctcgcg
     2101 cgcctggtga aaggcgtcgt tgaggcagta ttccagttcg ttcgacagca cgtcagtcca
     2161 gcctttcaga agttcagttc actcgccgca gtccctgcgc gtgccgatca ggcctcttcc
     2221 agggtgcaca tcagcggatg ctggtgatcc cgggcgtagg tcgtcacctg cgccacctta
     2281 gtttcagcga tctcgtaagt gaagaccccg cagacgccct tacccttggt atggacctcg
     2341 agcatgatcc gggtggcggt cggacggttc agcccaaaga acttttccag cacatcgacc
     2401 acaaactcca tgggggtgta gtcgtcattg agcagtacga cccggtacag cggcggctgc
     2461 ttaacctccg gttgcgcctc ttctacggct atgccggagt cggggcggac cgtatgctgc
     2521 tcgggcatgc gttctttata ggggtcgtga aacggcaaca caagagcggg acaatgtcac
     2581 ctggttact
//
LOCUS       DATLKL010000449         1514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_197050, whole genome shotgun sequence.
ACCESSION   DATLKL010000449 DATLKL010000000
VERSION     DATLKL010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197050"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..492
                     /locus_tag="VM713_05990"
     CDS             40..492
                     /locus_tag="VM713_05990"
                     /inference="COORDINATES: protein motif:HMM:NF020413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HVH83848.1"
                     /translation="MATSDPYELARFLEAQSRCYAQVLEELAAGEKASHWMWFIFPQL
                     KGLGVSTTARRFGLSGLEEARAYLAHAVLGERLRQCTQLLLAVEGRSAHEIFGSPDDL
                     KLRSCLTLFAQAAPAPAQEDRVFVEALAKYYGGEPDPVTLRLLTSSVS"
     gene            complement(473..1234)
                     /locus_tag="VM713_05995"
     CDS             complement(473..1234)
                     /locus_tag="VM713_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83849.1"
                     /translation="MERAAILAAATLLALSAASPGAYAAPDSATPKYIANAVADPARP
                     AADRERDANRKPAETLAFAGVKPGDAVVELAPGKGYYTRLLSAVVGTKGKVYPVTQPP
                     KPDAPPPAVQAIAADPHYGNVQVTLTKYAEPKLPAAVDVVWTTQNYHDFHNIPDVQVA
                     TLNKAVFDALKPGGIFFILDHAAEAGSGARDTNTTHRIDEETVKEEVKAAGFELAGES
                     NILRNKDDPRTGKVFEPPIQGHTDQFVLKFRKPKK"
BASE COUNT          210 a          507 c          518 g          279 t
ORIGIN      
        1 gaccgaccgc ttcgtgctgg tgttccgcaa agccccttga tggcgacctc ggatccctac
       61 gagctcgcgc gcttcctcga agcacagtcg cgctgctatg cgcaggtgct ggaagagctc
      121 gccgcgggag agaaggcgag ccactggatg tggttcatct tcccgcagct caagggtctc
      181 ggcgtgagca cgacggcgcg gcgcttcgga ctcagcgggc tcgaggaggc gcgcgcctat
      241 ctggcgcacg cggtgctcgg cgagaggctc cgccagtgca cgcagctgct gctcgccgtc
      301 gaggggcgca gcgcccatga gatcttcggc agccccgatg acctgaagct gcgctcgtgc
      361 ctgacgctgt tcgcgcaggc ggcgcccgcg ccggcccagg aagatcgggt cttcgtcgag
      421 gcgctcgcca aatactacgg cggcgagccg gatcctgtga cgctcaggct ccttacttct
      481 tcggtttcct gaacttcagg acgaactggt cggtatgccc ctgtatcggg ggctcgaaca
      541 ccttaccggt gcgcgggtca tccttgtttc tgaggatgtt gctctcgccc gcgagctcga
      601 agccagccgc cttcacttcc tctttcaccg tctcctcgtc gattcggtgc gtcgtgttcg
      661 tgtcacgcgc gccggagccg gcctccgcgg cgtgatccag tatgaagaag atgccgcccg
      721 gcttcagcgc atcgaacacc gccttgttga gggtcgcgac ctggacatcc ggaatattgt
      781 ggaagtcgtg atagttctgg gtggtccata cgacgtccac agcggcgggc agcttcggct
      841 cagcgtactt cgtcagcgtc acctgcacgt tgccgtaatg cgggtcggcc gcgattgcct
      901 gcaccgcggg aggcggtgcg tccggtttcg gcggctgcgt cacggggtac accttgcctt
      961 tcgtgcccac caccgcgctc agcaggcggg tgtagtagcc cttgcccggt gccagctcca
     1021 ccaccgcatc gccgggcttg acgccggcga aggcgagcgt ctccgcgggt ttgcggttcg
     1081 cgtcgcgctc gcggtcggct gcggggcggg ccgggtccgc gactgcgttg gcgatgtatt
     1141 tcggggtcgc gctgtcgggg gccgcatagg cgcccgggct cgcggcgctc aaggcaagaa
     1201 gggtcgcggc ggcgagaatc gctgcacgtt ccatggatcg cctcctcgag aaaggagctc
     1261 gctgctccga agggcgcgcc actgtagcag gagtccgcac cgcctcacta tcgcttcggc
     1321 gcgccgcgcc ggcggtacgc cgcgcgctgt gtcagatggg cgaagtaacg cggcccgccg
     1381 ctcgcgagag cccccttcag gtcgccaggc gttgagtgag gcgctgccgc accccgggcc
     1441 actcggacgc gacggtcttg ccccggaaat tcttccggaa cgcctgcgcc gccatggtgc
     1501 cgagaaccgt cgag
//
LOCUS       DATLKL010000450         1986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_185851, whole genome shotgun sequence.
ACCESSION   DATLKL010000450 DATLKL010000000
VERSION     DATLKL010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185851"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1986
                     /locus_tag="VM713_06000"
     CDS             <1..>1986
                     /locus_tag="VM713_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVH83850.1"
                     /translation="LCVLAALSSTPGSAAELNPARTHPRGTTAVAAEQLIIKLRAGPA
                     TTAASTDAAGGARERVATLAARNGMRLVRHRPITALIHAVHIETAGGESAPAVLARLR
                     ADPEVEYAVTDERRYIHGAPNDPLYAQQWYLQTAAATPSAVDALDAWSTTTGSATLVI
                     ADIDTGVRPDHPDLAGRLLSGYCFISDAIVVNGGSCPGPGAADPGDWVTSSDITNDPT
                     ECGQATAGPSSWHGTRVAGMLGALTNNGIGVAGLTWGSPILPVRALGKCGGLDSDIIT
                     AMLWAGGIAVSVNGANVTNPNPAKIINMSLGGAGACPQSYIEAIGQLSALGVLVVASA
                     GNEGGPVDAPANCPGVASVAGLRQAGTKVGYSSLGPEVALGAPAGNCGNSFTGGQTAC
                     VYSMTSTTNLATMGPATPDANDYTGLYHCDATSGSYTGCTISSGQYRTYSIGTSFSAP
                     VVSGIAALMSSVNSSLNSCQLISRLKEGSLPYPQTSLDAAGAQPAMCHVPASASDVQN
                     TECICTRNDQTCGAGMANAPGALTAALRPIAAVAVPASVTAGQSVTLDGSASGAANGH
                     IVSSYQWSPVSGGLTLSVTGAATAKASITAPACGVGTVSLTVTADAGHSDTANVIVTP
                     GSVTTSAPAAAGQGACSSAAPAVLVEVCPAAGSVQAGG"
BASE COUNT          307 a          750 c          674 g          255 t
ORIGIN      
        1 cctctgtgtg ctcgccgcgc tgagcagcac gcccgggagc gccgcggagc tgaacccggc
       61 gcgcacccac ccgcgcggca caacggccgt ggcggcggag cagctcatca tcaagctgcg
      121 tgccgggccg gctaccaccg ctgcctccac agacgccgcg ggcggtgcca gggagcgcgt
      181 cgccacgctc gctgcgcgca acggcatgcg gctcgtgagg catcgcccga tcaccgcgct
      241 catccacgcc gtacacatcg agacggcggg cggagagtca gcgccggcgg tgctggcgcg
      301 actgcgcgcc gaccccgagg tcgaatacgc ggtaaccgac gagcgccgct atatccacgg
      361 cgcgccgaac gatccgctct acgcgcaaca gtggtacctg cagacggccg cggcgacgcc
      421 gagtgccgtc gatgcgctcg atgcttggag caccaccacc ggcagcgcca ccctcgtcat
      481 cgccgacatc gataccgggg tgcggccgga tcacccggat ctcgcgggcc ggctgctgtc
      541 cggctactgc ttcatttccg atgccatcgt cgtcaacggc ggcagctgcc cgggcccggg
      601 cgcggccgac cccggagact gggtcacgag ctccgacatc accaacgacc cgaccgagtg
      661 cggccaggcc accgcgggac cgagctcctg gcacggcacg cgcgtcgcgg ggatgctcgg
      721 ggctctcacc aacaacggca tcggcgtcgc cgggctcacc tggggctcgc cgatcctgcc
      781 ggtgcgcgcg ctcggcaagt gcggcggcct cgactccgac atcatcacgg cgatgctctg
      841 ggccggcgga atcgcggtca gcgtcaacgg cgccaacgtg accaacccga acccggcgaa
      901 gatcatcaat atgagcctcg gcggcgcggg cgcctgcccg cagagttata tcgaggccat
      961 cggccagctg tcggcgctcg gcgtgctggt ggtcgcctcg gccggcaacg aaggcggccc
     1021 ggtggacgca ccggccaatt gcccgggcgt cgcgtctgtg gcggggctgc gccaggccgg
     1081 gaccaaggtc ggctatagca gcttaggtcc cgaggtcgcg ctcggggcgc cggcgggcaa
     1141 ctgcggcaac tcgttcaccg gcggccagac ggcctgcgtg tactccatga cgagcacgac
     1201 caatctggcc acgatgggtc cggcgacgcc cgacgccaac gactacaccg gcctctacca
     1261 ctgcgatgcc accagcggca gttacaccgg ctgcaccatc agcagcgggc aataccgcac
     1321 ctacagcatc ggcacgagct tctccgcacc ggtcgtctcc gggatcgcgg ccctcatgtc
     1381 ctcggtcaac tcgagcctca actcctgcca gctcatctcg cggctgaagg aaggctcgct
     1441 gccgtatccg cagacctccc tcgatgcggc cggcgcccag cccgcgatgt gccacgttcc
     1501 ggccagcgcc agcgacgtgc agaacaccga gtgcatctgc acgcgcaacg atcagacctg
     1561 cggcgccggc atggccaacg cgccgggcgc cctcactgcg gcgctgcgcc ccattgccgc
     1621 cgtcgcagtc ccggcgagcg tcacggcggg ccagagcgtg acgctcgacg gcagtgccag
     1681 cggcgcggcc aacggccaca ttgtcagcag ctatcagtgg agcccggtga gcggcgggct
     1741 cacgctgagc gtcacgggcg cggcgacggc gaaggcttcg atcaccgcgc ccgcgtgcgg
     1801 tgtcggcacc gtctccctca ccgtcaccgc cgacgccggc cacagcgaca ccgccaacgt
     1861 gatcgtcacg ccgggatctg tgaccacgag cgcgccggct gcggccggcc agggcgcctg
     1921 ctcgagcgcc gcgcctgcgg tcctcgtcga ggtctgcccg gcagccggga gtgtgcaggc
     1981 gggagg
//
LOCUS       DATLKL010000451         1584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_152166, whole genome shotgun sequence.
ACCESSION   DATLKL010000451 DATLKL010000000
VERSION     DATLKL010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152166"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VM713_06005"
     CDS             <1..788
                     /locus_tag="VM713_06005"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170938.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HVH83851.1"
                     /translation="AEALAAFGNDQVYMEKFLERPRHIEFQVLADHHGNVIHLGERDC
                     SMQRRHQKEIEEAPAPGITLRQRKMMGDRCVEACRAVSYRSAGTLEFLYEDGEFYFIE
                     MNTRIQVEHPVTELITGIDLVKAQLLIASGERLPYLQSDVQIRGHAIESRINAEDPKT
                     FVPSPGPIRLWHAPGGPGVRVDSHVYSGYNVPPYYDSLIAKLITHGDSRDTAIARMMN
                     ALAEMVIEGIKTNVALHQEIFNHAAFQKGGTDIHYLERRLGLR"
     gene            832..>1584
                     /locus_tag="VM713_06010"
     CDS             832..>1584
                     /locus_tag="VM713_06010"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HVH83852.1"
                     /translation="MAASVTVSFDLSGLDAEGAESTCFACGAAAVTLVDSRDDPVLEP
                     APGEVRLWPATRVSALFLDAPEPRSLAAALAGRLGLPPERVSAELLPERAWEREWLRD
                     FHAMRFGERLWVCPRHERVGDPTAVVVTLDPGLAFGTGTHPSTALCLRYLDAHLAPGA
                     RVIDYGCGSGVLALACAKLGAAEVHCFDIDGQALIATRDNALENEVAGVVHVHERAEA
                     LPAAVDLLMANILSGPLCTLAASFAALVRPGGA"
BASE COUNT          246 a          554 c          534 g          250 t
ORIGIN      
        1 aggcggaggc gctcgcggcg ttcggaaacg atcaggttta catggagaag ttcctcgagc
       61 gtccgcgcca catcgagttc caggtgctcg ccgatcatca cggcaacgtg atccatctcg
      121 gcgagcggga ctgctcgatg cagcgccggc accagaagga gatcgaggag gcgcccgccc
      181 ccgggatcac cctgaggcag cgcaagatga tgggcgaccg ctgcgtcgag gcctgccgcg
      241 cggtcagcta ccgcagcgcc ggcacgctcg agttcctcta cgaggacggc gagttctact
      301 tcatcgagat gaacacgcgc atccaggtcg agcatccggt gaccgagctg atcaccggca
      361 tcgatctggt caaggcgcag ctgctcatcg cgagcggcga gcggctgccc tacttgcaga
      421 gcgacgtgca gatccgcggc catgccatcg agtcccgcat caacgccgag gaccccaaga
      481 ccttcgtgcc ctcgccgggg ccgatccgcc tgtggcacgc ccccggcggg cccggcgtcc
      541 gcgtcgacag ccacgtgtac tcgggctaca acgtgccgcc ttactacgat tcgctcatcg
      601 cgaagctgat cacgcacggc gacagtcgcg atacggctat cgcccgcatg atgaacgcgc
      661 tcgcggaaat ggtgatcgag ggcatcaaga ccaacgtcgc cctgcaccag gagatcttca
      721 accacgcggc cttccagaag ggcggcaccg acattcacta cctggagcgg cggctcggat
      781 tgcggtgagg acggtgacta gcgccctcag caagccgcgc ataatcggcg catggctgcg
      841 agcgtcacgg tctccttcga cttgagcggg ctcgacgcgg aaggtgcgga gagcacctgc
      901 ttcgcctgcg gcgcggccgc cgtcaccctg gtcgactcgc gcgacgaccc ggtgctcgag
      961 ccggcgccgg gcgaggtgcg gctgtggccg gcgacgcgcg tgagcgcgct gttcctcgac
     1021 gcgcccgagc cgcgctcgct cgcggccgcg ctcgcaggca ggctcggact cccgcctgag
     1081 cgcgtcagcg ccgagctgct gcccgagcgc gcctgggagc gcgagtggct cagggacttc
     1141 catgccatgc gctttggcga gcggctgtgg gtgtgtccgc ggcacgagcg cgtcggcgat
     1201 ccgaccgccg tcgtcgtgac gctcgatccc gggctcgcct tcggcaccgg cactcatccg
     1261 agcaccgcgc tgtgcctgag gtatctcgat gcgcatctcg caccgggcgc gcgcgtcatc
     1321 gattacggct gcggctcggg cgtgctggcg ctcgcctgcg ccaagctcgg cgcggccgag
     1381 gtccattgct tcgatatcga tggccaggcg ctcatcgcca cccgtgacaa cgccctcgag
     1441 aacgaggttg ccggcgtggt ccacgttcac gagcgtgcag aagctctgcc ggctgccgtc
     1501 gatttgctga tggcgaacat cctctccggg cctctgtgca cactggccgc aagcttcgcg
     1561 gcgctggtcc gcccgggcgg cgct
//
LOCUS       DATLKL010000452         1875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_208306, whole genome shotgun sequence.
ACCESSION   DATLKL010000452 DATLKL010000000
VERSION     DATLKL010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208306"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VM713_06015"
     CDS             <1..900
                     /locus_tag="VM713_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HVH83853.1"
                     /translation="GCYGHLTPSGALAQGALAGAPPDAGKAWREMIHRYAAGTLALLI
                     VALAALALGARRRRAVSVSFALALLATVIVQALLGMLTVTWRLKPLVVTLHLLVGMTT
                     LGMLWWLALSLPLSSWGASSLGGAGRSLRGGAAYIASAHHLALLWLVALALQIALGGW
                     TSSNYAATACPDFPTSQGAFWPHGDFRDAFVPWHAANVNYEGGLLGNPARVAIHLTHR
                     LGALLAAAALALAAVYTLRHRGLAGARLRAWAVLAALGLQLILGVSMVVRGFPLWLAT
                     AHTAGAALLLLAALALVRNLSAR"
     gene            927..1829
                     /gene="cyoE"
                     /locus_tag="VM713_06020"
     CDS             927..1829
                     /gene="cyoE"
                     /locus_tag="VM713_06020"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HVH83854.1"
                     /translation="MATSSDLASRAPPLWRRYLELTKPRVVALITFTAIVGTLLASPG
                     APPLEPLVFGNLGVALAAACAATINHVLDRRIDEQMARTRARPLPTGALTERAALVFA
                     AVLGVAAMSVLAFLVNLLTAVLTFCSLIGYAVIYTVWLKRATSQNIVIGGAAGAAPPV
                     LGWTAVTNSVDPNALLLFLIIFVWTPPHFWALAIARRDEYARAGIPMLPVTHGVKFTR
                     LQVLLYTVLLTVVTLLPWLTRMSGLVYLAAALVLNTGFLYYALALKVSAREELPMRVF
                     RFSVNYLMWLFAALLVDHYLPALR"
BASE COUNT          207 a          701 c          638 g          329 t
ORIGIN      
        1 ggctgttacg ggcatctcac tccgagcggc gcgctcgcgc agggtgcgct cgctggtgcg
       61 ccgcccgatg ccggcaaggc gtggcgcgag atgatccacc gctatgctgc cggcacgctg
      121 gcgctcctca tcgtcgcgct cgcggcgctc gcgctcggcg cacgtcgccg gcgcgcggtg
      181 agcgtgtcct tcgccctcgc gctgctggcg actgtgatcg tgcaggcgct gcttggcatg
      241 ctaaccgtga cctggcggct caagcccctc gtcgtgacgc tgcacctcct ggtcggcatg
      301 acgacgctcg ggatgctgtg gtggctcgcg ctgtcgctgc cgctctcgtc ctggggcgcg
      361 agctcgctcg gcggcgcggg ccgctcgtta cgcggcgggg cggcttatat cgcgagcgcg
      421 caccacctgg cgttgctctg gctcgtggca ctcgcgctgc agatcgcgct tggcggctgg
      481 accagcagca actatgccgc caccgcctgt cccgacttcc ccaccagcca gggcgccttc
      541 tggccgcacg gcgactttcg cgacgccttc gtgccgtggc acgcggcgaa cgtcaactac
      601 gagggggggc tcctcggcaa ccccgcgcgc gtcgccatcc acctcaccca ccgcctgggg
      661 gcgctgctcg ccgccgcggc gctcgcgctc gccgcggtgt acacgctgcg ccatcgcggc
      721 ctcgccggag cgcggctgcg cgcctgggcg gtgctcgcgg cgctcggcct gcagctcatc
      781 ctcggggtct ccatggtggt gcggggcttc ccgctgtggc tcgccacggc gcacacggcc
      841 ggcgcggcgc tgctgctgct cgcggcgctc gcgctggtga gaaacttaag cgcacgctag
      901 gctgcgcata ccgcgaagtg agcatcgtgg cgacctcatc ggaccttgcg agccgcgcgc
      961 cgccgctctg gcggcgctac ctggagctga ccaagccgag ggtcgtcgcg ctcatcacct
     1021 tcaccgcgat cgtcggcacg ctgctcgcga gcccgggcgc gccgccactc gagccgctgg
     1081 tgttcggcaa cctgggggta gcgctcgccg ctgcctgcgc cgccaccatc aatcacgtgc
     1141 tcgaccggcg gatcgacgag cagatggcgc gcacgcgcgc gcgcccgctg ccgaccggcg
     1201 cgctcaccga acgcgccgcg ctcgtcttcg cggcggtgct cggcgtcgcc gccatgtcgg
     1261 tgctcgcgtt cctggtgaac ctcctcaccg cggtgctcac cttctgctcc ctgatcggct
     1321 acgcggtcat ctacaccgtg tggctgaagc gcgcgacctc gcagaacatc gtgatcggag
     1381 gagccgccgg cgccgctccg ccggtgctcg ggtggacggc ggtcaccaac agcgtcgatc
     1441 cgaacgcgct gctgctgttc ctcatcatct tcgtgtggac gccgccgcat ttctgggcgc
     1501 tggcgatcgc gcgccgcgac gagtacgcgc gcgccgggat cccgatgctg ccggtgaccc
     1561 acggcgtcaa gttcacgcgg ctgcaggtgc tgctctacac cgtgctgctc accgtggtga
     1621 cgctgctccc ctggctcacg cgcatgagcg ggctggtcta tctcgccgcc gcgctcgtcc
     1681 tcaacaccgg cttcctctac tacgcgctgg cgctcaaggt gagcgcgcgc gaggagctgc
     1741 cgatgcgcgt gttccgcttc tcggtcaact acctcatgtg gctgttcgcg gcgctgctcg
     1801 tcgatcacta cctgccggcg ctgcggtagt tggattgtgc tcaggtctcg cggctgagcc
     1861 gcatcgccca ggagt
//
LOCUS       DATLKL010000453         1655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_27845, whole genome shotgun sequence.
ACCESSION   DATLKL010000453 DATLKL010000000
VERSION     DATLKL010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27845"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1655)
                     /locus_tag="VM713_06025"
     CDS             complement(<1..>1655)
                     /locus_tag="VM713_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HVH83855.1"
                     /translation="VRGEIERLLHDSTAETEAEPAPKKPSKTPLLDQFGRDLTELARR
                     NQLDPVIGRETEIERVIQILSRRTKNNPALIGEPGVGKSAIAEGLAQQINFGNVPESL
                     QHKRVLTLDMGALVAGTKYRGVFEERLKKILDEIRSSREVVLFIDELHTLVGAGAAEG
                     AIDAANILKPALARGEIQCIGATTLNEYRKYIEKDAALERRFQPVYVDQPTLSETIDI
                     LNGVKSLYEKHHRVTITDAAVRSAAVLSDRYVSDRALPDKAIDLIDEASARVRMKLTT
                     TPEEHKDLQKEIRRQRVEQDESVTKQDFEAAAGVRDKVKKLQDKLAVKEAQWRDKLGE
                     TVPDVGEEDIAQIVAAWTGVPVSRLVEEESARLLRMEDELHKRMVGQDEAIVTVSQAV
                     RRARAGLKNPRRPIGSFIFLGPTGIGKTELARALAEYLFDSEDALIKLDMSEFMERHT
                     TARLVGSPPGYVGYDEGGQLTEAVRRRPYSVILFDEIEKAHPEVFNMLLQILEDGRLS
                     DAKGKIVNFANTVIIMTSNLGIRDVNQAGTSLGFQPAVVDEKD"
BASE COUNT          234 a          562 c          528 g          331 t
ORIGIN      
        1 gtccttctcg tccacgaccg cgggctggaa cccgagggat gtccccgcct ggttgacgtc
       61 gcggataccg aggttcgagg tcatgatgat cacggtgttc gcgaagttga cgatcttgcc
      121 cttcgcgtca cttagccttc cgtcctcgag gatctggagc agcatgttga agacctccgg
      181 gtgcgccttc tcgatctcgt cgaaaaggat caccgagtaa ggccgcctgc ggaccgcttc
      241 ggtgagctgg ccgccctcgt cgtagcccac gtagccggga ggcgaaccga ccaaccgcgc
      301 ggtggtgtgc cgctccatga actccgacat gtcgagcttg atcagcgcgt cctcggagtc
      361 gaagaggtac tcggccagcg cgcgggccaa ctccgtcttg ccgatgccgg ttggccccag
      421 gaagatgaac gatccgatcg gccggcgcgg gttcttgaga ccggcgcggg cgcggcgcac
      481 cgcctgcgac accgtgacga tcgcctcgtc ctgaccgacc atgcgcttgt gcagctcgtc
      541 ttccatgcgc aggagccgcg cgctctcttc ctcgacaagg cgtgagaccg gaactccggt
      601 ccacgccgcc acgatctggg cgatgtcttc ctcgcccacg tcgggcacag tctctccgag
      661 cttgtcgcgc cactgcgcct ctttgaccgc cagcttgtcc tggagcttct tgaccttgtc
      721 gcgcacgccc gcggccgcct cgaagtcctg cttggtcacg gactcgtcct gctcgacgcg
      781 ctgccggcgg atctccttct ggaggtcctt gtgctcctcc ggcgtcgtag tgagcttcat
      841 gcgcacgcgc gccgacgcct cgtcgatcag gtcgatcgcc ttgtcgggca gcgcccggtc
      901 gctcacgtag cggtcgctca acaccgccgc cgagcgcaca gccgcgtccg tgatggtcac
      961 gcggtggtgc ttctcgtaca gcgacttgac gccgttgaga atgtcgatcg tctcgctgag
     1021 cgttggctgg tcgacgtaga cgggctggaa gcgtcgctcg agggcggcgt ccttctcgat
     1081 gtacttgcgg tactcgttga gcgtggtcgc gccaatgcac tgaatctcgc cgcgcgccag
     1141 cgccggcttc agaatgttcg ccgcatcgat tgcgccttcc gccgcgccgg ccccgacgag
     1201 cgtgtgcagc tcgtcgatga agagcacgac ctcgcgactc gagcggatct catcgaggat
     1261 cttcttcaac cgctcttcga acacaccgcg gtacttggtg cccgcgacca gcgcacccat
     1321 gtccagcgtc agcacgcgct tgtgctgcag cgactcgggc acgttgccga aattgatctg
     1381 ctgtgcgaga ccctcggcga tcgcggactt gccaacgccc ggctcgccga tgagcgcggg
     1441 gttgttcttg gtgcggcggc tcaggatctg gatcacgcgt tcgatctcgg tctcgcgtcc
     1501 gatcacgggg tcgagctgat tgcgtcgcgc gagctcggtc aagtcgcggc cgaactggtc
     1561 cagcagcgga gtcttggagg gcttcttcgg cgcgggctcg gcctcggtct cggccgtcga
     1621 gtcgtgcagc aggcgctcga tctcgccacg cacct
//
LOCUS       DATLKL010000454         4081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_185910, whole genome shotgun sequence.
ACCESSION   DATLKL010000454 DATLKL010000000
VERSION     DATLKL010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185910"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VM713_06030"
     CDS             complement(<1..698)
                     /locus_tag="VM713_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HVH83856.1"
                     /translation="MSVVTPDEMHANDTGTVSAERRLSFAFAKRHGVLVRSIAEGVAD
                     CVCREDASPLALAEVRRYLRRPVKLERVPEEEFDLLLRRAYEAGSDAMQAVEGLDETT
                     DLAHLAQELPEQADLLDSEDDAPIIRLINAVLTQAVKENASDIHIEPFENRLVIRFRV
                     DGVLREVLQSKRAVAPLVVSRVKVMSRLDIAEKRLPQDGRISLRIAGRAVDVRVSTIP
                     SGHGERVVLRILDK"
     gene            complement(712..2844)
                     /gene="gspD"
                     /locus_tag="VM713_06035"
     CDS             complement(712..2844)
                     /gene="gspD"
                     /locus_tag="VM713_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HVH83857.1"
                     /translation="MTRRRSFAPCALAILLAGSLATAQPEQQVQRITPNFKDADITQV
                     AEAVAAATGKIFIIDPRVRAQVTMLSSTSMSPAAFYEAFLSILQVYGFIAVPQGNIVK
                     IIPDTNAKTSPSVDLPEHVSATSDVIVTQVLDVKNVSAAQLVPILRPMVPQYGSLVAY
                     PAGNILVITDHASNVNRMIRIIQRIDTVGNQDPEIVPLSNASAAEIVRVVNSLYQGAA
                     AEGSTVKVVADERSNTVLIGGQEAQRLRIRALIAHLDTPLGGGGDTRVRYLHYANAEK
                     LAPKLKEQITGIAQAAAGPAGPTAAAANPLAQAEKNAMVWADPTNNALVITAPPKIMR
                     AVMDIVDKLDIRRPQVLVEAIIADINFDKDAELGVNWAAFINGTNVPAGAFVSPVGGT
                     SIVDLAGAIQNPANATTTLLQGTTLGIGRIGGSSGTNFAAVLRAIRADTNDNVISTPS
                     AVTMDNQEAELKVAQEVPFITGQYTANNTAVVGGAVNPFQTIQREEVGTILKITPTIS
                     PEGTAVMLKISIESSTIGQKPAGAVDLVTNKRTVSTTVMIDDGGIVVLGGLTEDDRTK
                     GENRVPFLGNIPIIGYLFKTRNEQSTKNNLMIFIRPKIMRDAAQAAYETDLKYNYMLD
                     QERQIDKASREALPMLPGVPRGTLPPLPPGGSAPAPRPQGSTPPTSQGTPANNPPITM
                     PPPGPAVAPGTAAPESPTAAPAEPPRKQ"
     gene            complement(2878..3969)
                     /gene="gspC"
                     /locus_tag="VM713_06040"
     CDS             complement(2878..3969)
                     /gene="gspC"
                     /locus_tag="VM713_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HVH83858.1"
                     /translation="MNAASWLEGLPAPQRWRALLRAEGPRAATWALALALGVQAAFIV
                     TDLAGAGGGRRTAAAASQPFTPHFTDVAAITNAHLFGAAPVPKLSEADAPQTSMPLVL
                     TGIIAANDPQNGLAILGPTAQTAKVHAVGDSVPGGARLHAVYSDRVIIDRNGQLESLV
                     LPRQTGGNAPAPSTASLPTVSPVVDRMRRALTEQPGLIADLLRPQAVIEDGRVKGFRV
                     FPGRNRLAFARLGLRPGDEVVAINGTPLDDQDRGQAILNTLGTSPDARVTVIRNNQQQ
                     DLTLNIAQVAQEAETATGLQPAGPGAPPPLPGASQFFAPAQPSAPAPPGAAPEIAPPP
                     PPAASPGAATVPINTSSPEPGTPEGTQPQ"
BASE COUNT          576 a         1331 c         1465 g          709 t
ORIGIN      
        1 tgcttgtcga gaatgcgcag caccacgcgc tcgccgtgtc cggaggggat ggtcgacacg
       61 cgcacgtcga ccgcgcggcc ggcgatgcgc aggctgatgc gcccgtcctg cggcagacgc
      121 ttctcggcga tgtcgagccg cgacatgacc ttcacccgcg acaccacgag cggcgccacc
      181 gcgcgcttcg actggagcac ctcgcgcagc accccgtcga cgcggaaacg gatcaccagc
      241 cggttctcga acggctcgat gtggatgtcg gaggcgttct ccttcaccgc ctgggtgagc
      301 accgcgttga tcaggcggat gatcggggcg tcatcctcgc tgtcgagcag gtccgcctgc
      361 tcgggcagct cctgcgcgag atgcgcgagg tcggtggtct catcgaggcc ctcgaccgcc
      421 tgcatggcgt ccgagccagc ctcataggcg cgccgcagca gcaggtcgaa ctcctcctcc
      481 ggcacccgct cgagcttcac cggacggcgc agatagcggc gcacctcggc gagcgcgagc
      541 ggcgaggcgt cctcgcggca cacgcagtcg gcaacccctt cggcaatgct gcgcaccagc
      601 accccgtggc gcttggcgaa cgcgaacgac aaccgtcgtt ccgcggacac cgtgcctgtg
      661 tcgttggcgt gcatctcgtc gggggtaacc acgctcatgc cgtgatgggg ctcattgttt
      721 ccgaggcggc tcggcgggcg cagcggtcgg gctctcgggc gcggccgtac caggtgccac
      781 cgcggggccg ggcggtggca tggtgatcgg gggattattc gccggcgtcc cctgactcgt
      841 gggcggtgtg cttccctgcg gccttggggc gggcgccgaa ccgcccggag gcagcggcgg
      901 cagtgtcccg cgggggacgc ccggcagcat cggcagcgct tcgcggctcg ccttgtcgat
      961 ctgcctctcc tgatcgagca tgtagttgta cttgaggtcc gtctcataag ccgcctgcgc
     1021 ggcatcacgc atgatcttcg ggcggatgaa gatcatcagg ttgttcttgg tcgactgctc
     1081 gttacgggtc ttgaacaggt agccgatgat cggaatgttg ccgaggaacg gcacgcggtt
     1141 ctcgcccttg gtcctgtcgt cctcggtgag gccgccgagc accacgatgc cgccgtcgtc
     1201 gatcatcacg gtggtgctga cggtgcgctt gttcgtgacc agatcgactg cgccggcggg
     1261 tttctgtccg atcgtagagc tctcgatcga gatcttgagc atcaccgccg ttccttccgg
     1321 ggagatggtc ggcgtgatct tgaggatcgt gccgacttcc tcgcgctgaa tcgtctggaa
     1381 cggattcacg gcgccgccca cgactgcggt gttgttggcg gtgtactggc cggtgatgaa
     1441 cggcacctcc tgcgcgacct tgagctccgc ctcctggttg tccatggtga ccgccgaggg
     1501 cgtggagatc acgttgtcgt tggtgtcggc gcggatcgca cgcagcacag cggcgaagtt
     1561 cgtgccgctg ctgccgccga tacggccgat gcctagggtc gtgccctgca gcagcgtcgt
     1621 ggtggcattg gcgggattct gtatggcgcc cgccaggtcg acgatgctgg tgccgccgac
     1681 cgggctcaca aaggcaccgg ccggcacatt ggtgccgttg atgaacgccg cccagttgac
     1741 accgagctcg gcgtccttgt cgaagttgat gtcggcgatg atcgcctcga ccagcacctg
     1801 cgggcggcga atatcgagct tgtcgacgat gtccatgacc gcgcgcatga tcttcggcgg
     1861 cgcggtgatg accagcgcgt tgttggtggg atccgcccac accatggcgt tcttctccgc
     1921 ctgggcgagc gggttggctg ccgcggcggt ggggccggcg gggccggcgg cggcctgcgc
     1981 gatgccggtg atctgctcct tgagcttcgg cgcgagcttc tcggcgttgg cgtaatgcag
     2041 atagcgcaca cgcgtgtcgc cgccgccgcc gagcggtgtg tcgagatgcg cgatgagcgc
     2101 gcggatgcgc agtcgctgcg cctcctgccc gccgatcagc accgtgttgg agcgctcatc
     2161 cgcgacgacc ttcacggtgc tgccctcggc ggcggctccc tggtagagcg aattgaccac
     2221 gcgcacgatc tcggccgccg aggcgttgct gaggggcacg atctcaggat cctggtttcc
     2281 gaccgtgtcg atgcgctgga tgatgcggat catccggttg acgttactgg catggtcggt
     2341 gatgaccagg atgttaccgg cggggtaggc gaccagcgag ccgtactgcg gcaccatggg
     2401 ccgcaggatc ggcacgagct gcgcggcact cacgttcttg acgtcgagca cctgcgtcac
     2461 gatcacatcg gaggtcgcgc tcacgtgctc gggcagatcg accgagggac tcgtcttggc
     2521 gttggtgtcc ggaatgatct tgacgatgtt gccctggggc accgcgatga agccgtatac
     2581 ctgcaggatc gacaggaacg cctcatagaa ggccgccggc gacatggagg tcgaggacag
     2641 catggtcacc tgggcgcgca cccgcgggtc gatgatgaaa atcttccccg tggccgccgc
     2701 caccgcctcg gcgacctgcg tgatgtcagc gtccttgaag ttgggcgtga tgcgctgcac
     2761 ctgctgctcc ggctgcgccg tggcgagcga gccggccagc aggattgcga gcgcgcatgg
     2821 agcgaacgag cggcgacgtg tcattgttgt tgaacccctc aaagcgtgcg gccccgccta
     2881 ctgaggctgg gtgccttccg gtgtgccggg ctccggagac gaagtgttga tgggaacggt
     2941 tgccgcgccc ggacttgcag ccggcggcgg cggcggcgcg atctcaggcg ccgcgcccgg
     3001 cggcgccggc gcgctcggct gggcgggcgc aaagaactga ctcgccccgg gcagcggggg
     3061 cggcgcgccg gggcctgcgg gctgcagacc cgtcgcggtc tcggcttcct gggccacctg
     3121 tgcgatgttc agcgtgaggt cctgctgctg gttgttgcgg atcacagtga cgcgcgcatc
     3181 gggcgaagtg ccgagcgtat tcaggatcgc ctggccgcgg tcctggtcat cgagcggcgt
     3241 gccgttgatg gcgaccacct cgtcccccgg ccgcagcccg aggcgcgcga aggccaggcg
     3301 gttacgtccg gggaagaccc ggaagccctt gacccggccg tcttcgatca ccgcctgcgg
     3361 ccgcagcaga tcggcgatca gcccgggttg ctcggtgagc gcgcggcgca tgcggtcgac
     3421 cacggggctc acggtcggga gcgaggccgt ggacggtgcg ggcgcgttgc cgcccgtctg
     3481 ccgcggcagg accagggact cgagctgccc gttgcggtcg ataatcacgc ggtcgctgta
     3541 caccgcatgc agccgcgcgc ctccgggaac actgtcgcca accgcatgca ccttggcggt
     3601 ctgtgcggtc ggtccgagga ttgccaggcc gttttgcgga tcattagcgg cgatgatccc
     3661 cgtgagcacc aggggcatgc tcgtctgcgg cgcgtcggct tcacttaact tcgggaccgg
     3721 ggccgccccg aacagatgtg cgttggtgat cgcggcgaca tctgtgaaat gcggcgtgaa
     3781 cggctgcgat gccgccgcgg cggtccgccg accgcctccg gcgcctgcca gatcggtgac
     3841 gatgaacgcc gcctggacgc cgagcgcaag cgccagggcc cacgtggccg cgcgcggccc
     3901 ctcggcgcgc agcagcgcgc gccatcgctg cggcgccggc aaaccctcga gccaggaggc
     3961 ggcgttcatg ggggcaaaag catgagctta agggaggtta acaagagttc ctcgcgcgtc
     4021 gcggagcgcc cgccgcgcag gggcgcccgc ggccgatccg cgcaatcata cgggccggct
     4081 g
//
LOCUS       DATLKL010000455         6523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_140849, whole genome shotgun sequence.
ACCESSION   DATLKL010000455 DATLKL010000000
VERSION     DATLKL010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140849"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VM713_06045"
     CDS             complement(<1..281)
                     /locus_tag="VM713_06045"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83859.1"
                     /translation="MSEPAWTRAEFETRLRERGQAYHIHHPFNVMLNSGRASREQIRG
                     WVANRFYYQINIPLKDAAILANCEERAVRRNWVQRILDHDGYGDDPGGI"
     gene            complement(278..1198)
                     /gene="pqqB"
                     /locus_tag="VM713_06050"
     CDS             complement(278..1198)
                     /gene="pqqB"
                     /locus_tag="VM713_06050"
                     /inference="COORDINATES: protein motif:HMM:NF003523.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HVH83860.1"
                     /translation="MRIRVLGSAAGGGFPQWNCNCHNCSGVRDGTVRAAPRTQSSIAV
                     AGPDGASWALVNVSPDILTQLKRHRELQPARAPRDSRLAGIVLVDAQIDHTAGLFMLR
                     ESVAPLAVWCTDAAYADLTGGNPIFRVLGHYCGIDRRRIDCAGEEFSVDGIAGVRWRA
                     IAVASKAAPYSPHRGAPIPGDNVALVITDAATGSAAVYAPGLGAMEPALWGALEGAAC
                     VLVDGTFWSDDEMVVQGLAAKRARDLGHLPQSGAGGMLEWLAQLPGTTRKILIHVNNT
                     NPILDEDSTERAVLERAGVEVACDGMEIVL"
     gene            complement(1358..1606)
                     /locus_tag="VM713_06055"
     CDS             complement(1358..1606)
                     /locus_tag="VM713_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83861.1"
                     /translation="MVALRLRRQKPVENEVLGTYSFPSRTVQVLKTQDDHILWTCDCD
                     KFRRQAGQREPLWCKHIAKAAARRSLERLTRRVAVGRG"
     gene            complement(1677..2279)
                     /locus_tag="VM713_06060"
     CDS             complement(1677..2279)
                     /locus_tag="VM713_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83862.1"
                     /translation="MTEEEQGLAKALGDLRSVLIAGESIEAWAIQRRMFAVAHRRVIV
                     AATSGRLIIITRPLLGGFELVTVRWQDLEEITLRVGIFGADLGIRAGKASDLASLATP
                     GAQRVDLKGLRKEQAQAVYRICQAQDQAWREKRRVRELEELRARSGGIQVTAGSPLSA
                     SAAAAGDDAVRRLQEAKRLLDARLITDAEYEAIKAKIVSP"
     gene            complement(2289..3101)
                     /locus_tag="VM713_06065"
     CDS             complement(2289..3101)
                     /locus_tag="VM713_06065"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HVH83863.1"
                     /translation="MWEEETADSFRRKLIWWLGGLVVVAAAGFALWYRFLFHPAQNAP
                     PAAAAPAAPAQAATEPAISHPLPAGAGGPEALPALNDSDPMVQESLMGVLGRPTIESL
                     LVPQNIVRHIVVTIDNLPRHKVAVELRPVKPTAGQTAVSTEGDLVVLSEANFQRYATF
                     VRAVQAADVKAVVANYQRLYPLFQQAYEDLGYPGKYFNDRLVEVIDHLLETPEVAPPI
                     QLEQPRVFYNYANAALEGRSAGQKLLIRMGPANARIIKAKLREFRADIVARK"
     gene            3258..>6523
                     /locus_tag="VM713_06070"
     CDS             3258..>6523
                     /locus_tag="VM713_06070"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVH83864.1"
                     /translation="MPAEFHPAVAGWFARTFDAATPAQESAWPAIAAGRHVLIAAPTG
                     SGKTLAAFLAVIDQLVRAAIEGHGLADETRVVYVSPLKALSNDIHRNLEAPLTGIADE
                     LATLGLPAHGIRTFVRTGDTPQHERTAMRKRPPHIVVTTPESLYILLGSDSGRRMLAS
                     TRSVIVDEIHAMAGTKRGVHLALTLERLEALAGRRLTRVGLSATQKPIEEVARFLTGA
                     GALRADGSPDCTIIDVGHVRNRDLQLEIPSAPLESVMSGEVWTQLYDRLAELVGEHRT
                     TLVFVNTRRQSERIARHLSERLGAEHVAAHHGSMAKESRLDAEARLKRGELRALVATA
                     SLELGIDIGEVDLVCQLASPRSIAAFLQRAGRSGHSVGGTPKARLFPLSRDDLVECAA
                     LLAAVRRGELDRLSVPTKPLDVLAQQIVAEAAAREWDEDGLFALVCRAYPYRDLRRSE
                     FDELVRMLAEGYSTRRGRHGALIHHDGVNHRIKGRRSARLTALTSGGTIPDTADYQVL
                     LEPQAQFVGTVNEDFAIESLQGDIFQLGNVSYRILRVEPGRIRVEDARGEPPSIPFWI
                     GEAPGRTDELSASVSRLRRELEARLDEGAEAASAWLSADTGISGPAAVQLTSYLAAAR
                     AALGALPTQQLVIFERFFDEAGGMQLVIHSPFGSRINRAWGLSLRKRFCRSFNFELQA
                     AATEDTIVLSLTTAHSFELAEVARYLHSNTVRPLLTQALCAAPMFPVRWRWSATIALA
                     LPRFRGGKKVPAPLQRMAAEDLLTAVFPDQVACAENLSGELEIPDHPLVRQAIRDCLE
                     EAMDIEGLERLLRGLEAGSIRSLARDLTEPSPLALEVLSARPYAFLDDAPLEERRTQA
                     VMSRRWLDPQSAADLGRLDPEAIARVRAEAWPDAANADELHDALVWLTFLTAAEVERH
                     AGWPELMTQLALERRVTRLAAEPVEIWVTAEQLPLFTALYPEAARDPQVVTPAAHARE
                     VTAEAALVEVVRGRLTGSGPVTAPALAALSGLAVPRIDAALAALEAEGFAMRGAFTPE
                     ASASVPEWCERRLLARIHRYTVKRLRAEIEPTEARDFLRFLFEWQRVTASGRMEGPDA
                     V"
BASE COUNT          968 a         2317 c         2220 g         1018 t
ORIGIN      
        1 tcgatgccgc ccggatcgtc gccgtagccg tcatggtcga ggatgcgctg cacccagttg
       61 cggcgcacgg cgcgctcctc gcagttggcg aggatcgccg catccttcag cgggatgttg
      121 atctggtaat agaagcggtt cgccacccag ccgcggatct gctcgcgcga ggccctgccg
      181 gagttgagca tgacgttgaa cggatggtga atgtggtagg cctgcccacg ctcgcgcagt
      241 cgggtctcga actccgcgcg cgtccaggcg ggctcgctca caatacgatc tccatgccgt
      301 cgcaggcgac ctcgaccccg gcgcgctcga gcacggcgcg ctcggtcgag tcctcgtcca
      361 ggatcgggtt ggtgttgttc acgtggatga ggatcttgcg cgtggtaccc ggcagctgcg
      421 ccagccactc gagcatgccg ccggcgccgc tctggggcaa atgaccaagg tcgcgggcgc
      481 gcttcgccgc gaggccctgc accaccatct catcgtcact ccagaaagtg ccgtcgacca
      541 gcacgcaggc ggccccctcg agcgcgcccc acagggcagg ctccatcgct ccgagcccgg
      601 gcgcgtagac agcggcgctg ccggtcgcag catccgtgat cacgagcgcg acgttgtctc
      661 ccgggatcgg cgcaccacgg tggggcgagt aaggcgcggc cttgctcgct accgcgatcg
      721 cccgccagcg caccccggcg atgccgtcga ccgagaactc ctcgcccgcg cagtcgatgc
      781 ggcgccggtc gatgccgcaa tagtgtccga gcaccctgaa gatcgggttg ccgccggtga
      841 gatccgcgta ggcggcatcg gtgcaccaga cggcgagcgg cgccaccgac tcgcgcagca
      901 tgaagagccc ggcggtgtgg tcgatctgcg catcgaccag cacgatgccg gccagacgtg
      961 agtcgcgcgg cgcgcgcgcg ggttgcagct cgcggtgacg tttgagctgg gtgaggatat
     1021 cgggggagac gttgaccagc gcccaactag ccccatcggg gccagcgacg gcgatcgagg
     1081 attgagtcct cggcgcggca cgcacggtgc cgtcgcggac gcccgagcag ttatggcagt
     1141 tgcagttcca ctggggaaag ccgccgccgg cggccgatcc cagtacccgg attctcaggt
     1201 atcagcgcct cgcgatgtac agggtgattt ccatgccgaa gcgcatgtcg atcgcccgcg
     1261 gagtttccca gcgcatgtcg cagctcctgt tgctgtggcg catcacgata ccgaaaaacc
     1321 taaggcgccg caaaggcggg gccgggctcg atcggcgcta gccgcgtccc accgccacgc
     1381 ggcgggtgag ccgctcgagc gagcgccgtg cggctgcctt ggcgatgtgc ttgcaccaga
     1441 ggggctcgcg ctgtccggcc tggcggcgga acttgtcgca gtcgcaggtc cacaggatgt
     1501 gatcgtcctg cgtcttcagg acctggaccg tgcggctcgg gaagctgtag gttccgagga
     1561 cttcgttctc gaccggcttc tgccggcgca ggcggagcgc gaccatgggc gcaagcgtaa
     1621 cgcacctgcc ctgtggatcg cctgtggatt agttgttgat gctcatgtat agcggctcag
     1681 ggcgacacga tcttcgcctt gattgcctcg tactcggcat cggtgatgag cctcgcatcg
     1741 agcagccgct tcgcttcctg aagtcgccgc accgcgtcat cgccggccgc agcggcgctc
     1801 gccgacagag gcgagccggc cgttacctgg atgccgcccg agcgcgcccg cagctcttcg
     1861 agctcgcgca cccgacgctt ctcgcgccag gcctgatcct gcgcctggca gatgcgatag
     1921 accgcctgcg cctgctcctt gcgcagaccc ttgaggtcca cgcgctgcgc gcctggggtg
     1981 gcgagcgatg ccagatcact ggccttgccg gcgcgaatgc cgagatcggc gccgaagata
     2041 ccgacccgca gggtgatttc ctccaggtcc tgccagcgca ccgtgaccag ctcgaagcca
     2101 ccgagcagcg gacgcgtgat gatgatgagc cggccgctgg ttgcggcgac gatgacccgg
     2161 cgatgcgcta cggcgaacat ccgtcgctgg atcgcccagg cttcgatcga ctctccggcg
     2221 atcagcaccg agcgcaggtc gccgagcgct ttagcgaggc cctgctcttc ttcggtcata
     2281 ggcgcgtttt acttcctggc gacgatgtcg gcgcggaact cgcgcagctt cgccttgatg
     2341 atgcgcgcgt tcgcaggtcc catgcggatc aggagcttct gcccggccga gcgtccttcg
     2401 agcgcggcgt tggcgtagtt atagaagacc cgcggctgct cgagctggat cggtggggcg
     2461 acttccgggg tctcgagcag gtgatcgatc acctcgacca gccggtcgtt gaagtacttg
     2521 cccgggtagc cgagatcctc gtaggcctgc tggaagagcg gatagagccg ctggtagttc
     2581 gcgacgaccg ccttgacgtc ggccgcctgc accgcccgca cgaaggtcgc gtagcgctgg
     2641 aagttggcct cgctcagcac caccaggtcg ccctcagtcg acaccgccgt ctgcccggcg
     2701 gtcggtttca ccggccgcag ctccaccgcc accttgtggc gcggcagatt gtcgatcgtc
     2761 accacgatgt gccgcacgat gttctgtggc accaacaggg actcgatcgt cgggcgtccc
     2821 aacacgccca tgagcgattc ctgcaccatc gggtcactgt cgttgagggc cggcaacgcc
     2881 tcgggaccac cagcacctgc gggcagcggg tgtgaaatgg cgggctcggt cgcggcctgt
     2941 gccggcgccg ctggcgcagc agcggccggc ggcgcgttct gcgccggatg gaacaggaac
     3001 cgataccaga gcgcgaaacc ggcggccgcg acgacgacca ggccaccgag ccaccagatg
     3061 agcttgcggc ggaatgaatc cgcagtctct tcttcccaca tcgtgagtga tccccacgtg
     3121 caaccaatga ggtcccgact ctactctttt gggtccatac gcgcatacag ctgccgttca
     3181 tgtcagctgc tgtacgttgc attaattcgt cgctcccgag ctcctcgcgc cacgtgcggt
     3241 gtgcttcaat gccgcccatg cccgccgagt tccatcccgc cgtcgccggc tggttcgccc
     3301 gcactttcga cgccgcaacc ccggcgcagg agtcggcctg gcctgcaatc gcagccggac
     3361 gccacgtgct gattgcggcg ccgaccggct cgggcaagac actggccgcg tttctcgcgg
     3421 tcatcgatca gctggtgcgt gctgcgatcg agggacacgg gctcgccgac gagacccgcg
     3481 tggtatatgt ctcgccgctc aaggcgctct ccaacgacat ccaccgcaat ctcgaagcgc
     3541 cgctcaccgg catcgccgac gagctcgcca cgctcgggct ccccgcgcac ggcatccgca
     3601 ccttcgtgcg caccggcgac acgccgcagc acgagcgcac ggcgatgcgc aaacgcccgc
     3661 cgcacatcgt ggtgaccacg cccgagtcgc tctacatcct cctgggctcg gactcaggcc
     3721 ggcgcatgct cgcgagcacg cgcagcgtga tcgtcgatga gattcacgcc atggcaggca
     3781 ccaagcgcgg cgtgcacctc gccctcaccc tcgagcggct cgaggcgctt gcgggacggc
     3841 gcctgacgcg cgtcggactc tcggccacgc agaagccgat cgaagaagtc gcccgcttcc
     3901 tcaccggcgc gggcgcgcta cgtgccgacg gcagccccga ctgcaccatc atcgacgtag
     3961 gccacgtccg caatcgcgac ctgcagctcg agatcccgtc cgcgccgctc gagagtgtca
     4021 tgtcgggcga ggtgtggacg cagctctacg accgcctcgc ggagctcgtg ggcgagcatc
     4081 gcacgacgct ggtgttcgtc aacacgcgtc gccagtccga gcgcatcgcc cgccacctgt
     4141 cggaacggct gggcgccgag cacgtcgcgg cccatcacgg cagcatggcc aaggagtcgc
     4201 gactcgatgc cgaggcgcgc ctgaagcgcg gggagctgcg cgccctggtg gcgaccgcat
     4261 cgctcgagct cggcatcgac atcggcgagg tggacctcgt gtgccagctc gcctcgccgc
     4321 gctcgatcgc cgccttcctg cagcgcgccg gccgctccgg ccactcggtg ggcgggacgc
     4381 cgaaggcgcg cctgttcccg ctgtcgcgcg acgacctggt ggagtgcgcc gcgctcctgg
     4441 ccgccgtgcg ccgcggcgag ctcgaccggc tcagcgttcc gaccaagccc ctcgatgtgc
     4501 tggcacagca gatcgtggcg gaggccgcgg cgcgcgagtg ggacgaggac ggactgttcg
     4561 cgctcgtgtg ccgcgcctat ccctaccgcg atctcaggcg cagcgagttc gacgagctcg
     4621 tgcgcatgct ggcggagggc tacagcacgc gccgcggccg ccacggggcc ctcatccacc
     4681 acgatggcgt caaccaccgc atcaagggcc ggcgcagcgc gcgcctcacc gctctcacct
     4741 cgggcggcac cattcccgac accgccgact accaggtgct gctcgagccg caggcgcagt
     4801 tcgtcggcac tgtcaacgag gacttcgcga tcgagagtct gcagggcgac atcttccagc
     4861 tcggcaacgt gtcgtaccgc atcctgcggg tcgagccggg ccggatccgc gtcgaggatg
     4921 cgcgcggcga gccaccgagt attcccttct ggatcggcga ggccccggga cgcacggacg
     4981 agctctcggc gagcgtctcg cggctgcggc gtgagctcga ggcgcggctc gatgagggcg
     5041 cggaggccgc cagcgcctgg ctcagcgcgg acaccggcat cagtgggccg gcggcggtgc
     5101 agctcacgag ctacctcgcc gcggcgcgcg ccgctctcgg cgccctgccg acacagcagc
     5161 tggtgatctt cgagcgcttc ttcgacgaag ccggcggcat gcagctcgtg atccactcgc
     5221 cgttcggcag ccgcatcaat cgcgcctggg gcctcagctt acgcaagcgc ttctgccgct
     5281 ccttcaactt cgagctgcag gccgccgcca ccgaggacac gatcgtgctg tcgctcacca
     5341 cggcgcacag cttcgagctc gcggaggtcg cccgttacct gcattccaac accgtgcggc
     5401 cgctgctcac tcaggcactg tgcgccgcgc cgatgttccc ggtgcgctgg cgctggagcg
     5461 ccaccatcgc gctcgccctg ccgcgcttcc gtggcggcaa gaaggtcccc gcaccgctgc
     5521 agcgcatggc ggccgaggac ctgctcaccg cggtgttccc cgaccaggtg gcgtgcgccg
     5581 agaacttgag cggcgagctg gagatccccg accaccccct ggtgcgccag gcgatccggg
     5641 attgcctcga ggaggccatg gacatcgagg ggctcgagcg gctcctgcgc ggactcgagg
     5701 cgggcagcat ccgctcgctc gcccgcgacc tgaccgagcc ctcgccgctc gcgctcgagg
     5761 tgctgtcggc gcggccgtac gcgttcctcg atgatgcgcc actcgaagag cggcgcacgc
     5821 aggcggtcat gagccggcgc tggctcgacc cgcagagcgc cgcggattta ggcaggctcg
     5881 acccggaggc gatcgcgcgg gtgcgcgccg aggcgtggcc cgatgcggcg aacgccgatg
     5941 agctgcacga tgcgctcgtg tggctcacgt tcctcacggc ggccgaagtc gagcgccacg
     6001 caggctggcc ggagctgatg acccagctcg cgctcgagcg gcgcgtgacg cggctcgccg
     6061 ccgagccggt cgagatctgg gtgacggccg agcagctgcc gctcttcacc gcgctctacc
     6121 cggaagcggc gcgcgacccg caggtagtca caccggccgc gcacgcgcgc gaggtcacag
     6181 cggaggctgc gctcgtcgag gtggtgcgcg gccgcctcac gggatcggga ccggtgaccg
     6241 cgccggcgct cgccgcgctg agcgggctcg ctgtgccgcg catcgacgcg gcgctggcgg
     6301 cgctcgaggc cgagggcttt gcgatgcgcg gcgcgttcac gccggaggcg tccgcgagcg
     6361 tgcctgagtg gtgcgaacgg cgcctgctcg cccgcataca ccgctacacc gtaaagcggc
     6421 tgcgcgccga gatcgagccg accgaggcgc gcgatttcct gcgcttcctg ttcgagtggc
     6481 agcgcgtcac ggcgagcggc cgcatggaag gtccggatgc cgt
//
LOCUS       DATLKL010000456         1958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_95682, whole genome shotgun sequence.
ACCESSION   DATLKL010000456 DATLKL010000000
VERSION     DATLKL010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95682"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VM713_06075"
     CDS             complement(<1..350)
                     /locus_tag="VM713_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83865.1"
                     /translation="MALGGQTTVIQRILERRSERPAEAHDGTTATLAVMVRVLPAPKA
                     LLACHDPTLRAQLMRRIAPDVFQIEAVASDQEALQQFAAEYRPVVMTDSLELVRQLRS
                     RKAARNPFVLYIAEL"
     gene            433..999
                     /locus_tag="VM713_06080"
     CDS             433..999
                     /locus_tag="VM713_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HVH83866.1"
                     /translation="MIAAMEPPGITRHRRPFLAPLWVTLLALLIAAAVAYTVYRRAAT
                     TVVVLASSGEIDPATIADPPISAEGERRAQRLAQLFTASGGPGRLDALYVSGDRRAQQ
                     TAAPLAERLHLMPTQYDARDAAAAAAGLLRAHEGEAVLVVGGTAAVQQFLRQLSGAAG
                     APQWADPDLLYIVSVPSFGRPQVLLLKY"
     gene            complement(1014..1496)
                     /locus_tag="VM713_06085"
     CDS             complement(1014..1496)
                     /locus_tag="VM713_06085"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVH83867.1"
                     /translation="MLAMGARAPEFTLPDQDDNSESLSTLLRHGSLILYFYPADFTSG
                     CTREACTLRDLHGEVLQAGLDVAGVSPQDPASHRAFREKYQLPFTLLSDVDKSVIRMY
                     EVRGPFGFGVRRATYLIDQARYIRAAVLADFRIAEHEDFVRKAVALSAGTARLRRLQN
                     "
     gene            1723..1798
                     /locus_tag="VM713_06090"
     tRNA            1723..1798
                     /locus_tag="VM713_06090"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1756..1758,aa:Val,seq:cac)
BASE COUNT          312 a          636 c          672 g          338 t
ORIGIN      
        1 agctcggcga tgtaaagcac gaaaggattg cgcgctgcct tgcgtgaacg cagctggcgg
       61 acgagctcga gactgtcggt catcaccacg ggccgatact cggcagcgaa ctgctgcagc
      121 gcttcctgat ccgacgcgac cgcctcgatc tggaagacgt cgggcgcgat gcgccgcatc
      181 agctgcgcgc gcagcgtcgg gtcgtggcag gcgagcagcg ccttgggcgc cggaagcacg
      241 cgcaccatca ccgcgagtgt cgcggtggtt ccgtcgtgtg cctcggcggg acgctcggag
      301 cgccgttcga ggatgcgttg gatgaccgtc gtctggccgc cgagcgccat tacgccccct
      361 aagtcgcgaa tgccttaggc agagattact cgatacggcg ggcaaaatac ccgtgcgcgt
      421 ggcggtcaca ctatgattgc cgccatggaa ccgccgggca tcacccgcca ccgacgccca
      481 tttctggcac ccctgtgggt gacgctgctg gcgctgctca tcgcggcggc tgtcgcgtac
      541 accgtgtatc ggcgcgccgc gacgacggtg gtcgtgctcg catcctcggg ggagatcgat
      601 ccggcgacga tcgccgatcc gccgatctcg gccgagggtg agcggcgggc tcagcgcctg
      661 gcgcagctgt tcaccgcgag cggcggcccg gggcgcctcg acgcgctcta tgtgagcggg
      721 gaccggcgcg cgcagcagac ggcagctccg ctggccgagc ggctgcatct catgccaacg
      781 cagtacgacg cgagggatgc tgccgcggcc gcggcgggcc tgctccgcgc ccacgagggg
      841 gaggcggtgc tggtggtcgg cgggactgcc gctgtgcagc aatttctgcg gcagctgagc
      901 ggtgccgccg gtgcgccgca atgggccgac cctgacctct tatatatagt cagcgtgccg
      961 agcttcggcc ggccgcaagt gctgcttctc aaatactgag gcgcgatgcc cgctcagttc
     1021 tgcagacgcc gcaagcgcgc ggtgccggcg ctcaacgcga ccgccttgcg tacgaaatcc
     1081 tcgtgctccg cgatgcggaa gtccgccagc acggcagcgc ggatgtagcg cgcctgatcg
     1141 atgaggtagg tggcgcgacg gacgccgaat ccgaacggcc cgcgcacctc gtacatgcgg
     1201 atgacggact tgtcgacgtc cgagaggagc gtaaagggca actggtactt ttcgcggaag
     1261 gcgcgatggc tcgcgggatc ctgggggctc acgcccgcga catccagccc ggcctgcaga
     1321 acctcgccgt gcagatcgcg caacgtgcat gcctcgcgcg tgcagccgga ggtgaaatcg
     1381 gccggataga aatagaggat gagcgatccg tgccgcagca gcgtgctgag cgactcgctg
     1441 ttgtcatcct ggtccggcaa cgtgaactcc ggcgcccggg cgcccatcgc cagcataaga
     1501 gctcccccgc agacctgacg gcgcatcata gccgcatcgg gggacggtgc gggttccgcg
     1561 gccgaacggt gctggcctgg cacgccggga gccggcccgt gcaaattgga ccggcccccc
     1621 tgcgatgcta gaatcgcgcg ccttaaccgg gcccccgcga aatccgcgtc ttgatgagct
     1681 tgctcgcgct ctgcgatgcg cgtgatttcg ggggtggtta gtggggcggt agctcagctg
     1741 ggagtgcgtc gcgttcacaa tgcgaaggtc gagggttcga tccccttccg ctccaccatt
     1801 tacccctcta cggctttccg acggcgtcca gcctcgtcca tctctgattc gctatattta
     1861 ataaattcaa tgtcttatac tccattcgcg tccggaggcg tcttggtgag tcgatcgcaa
     1921 gggcggccct agactggggt atcctgtggg gtatcctt
//
LOCUS       DATLKL010000457         2263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_106858, whole genome shotgun sequence.
ACCESSION   DATLKL010000457 DATLKL010000000
VERSION     DATLKL010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106858"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1835)
                     /locus_tag="VM713_06095"
     CDS             complement(<1..1835)
                     /locus_tag="VM713_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVH83868.1"
                     /translation="MIKVEQLKLWICAQFLIIIAPITLVLFYQAVSDLRRTTAVERVV
                     ARQGLSRAMTDSFESFMTHAADAVDTGSLGRAAYEALQRSLAAAVALHKKDEEAAAVA
                     TGLQDLTGRISVTMPVEELLRLQPQIRQIRAAIGTLDSGYEQASGAAIGQAIDSARRQ
                     SKIVAVATVFTLLVAAWFLYSMITGVTEPLNAAVGLAGRIAAGEFAAAGSLPKRDLGN
                     LLASLRLMSEKLHESQLEIAEDQRRLEQRVAERTAEVEARTRELTRSVTELQALSEVG
                     QAVSSSLELETVLATVIERAVHLADADSGTLYSFSDTDGVFDPRANFGVSPDAIENLR
                     GRRIGLRDGPLGTCAVRRAPVQVTDVELFNQNLKNLRLYEGIHGVIAVPLLREDHVLG
                     ALLIRRKSKGEFPPSTVRLLQTLASQSVLAIENARLFKESEAKSEQLAQVSKLKSQFL
                     ANMSHELRTPLNAIIGLTEMLHEDARELNRTEEVEPLERVLRAAHHLLELINDILDLS
                     KIEAGRMDLHVQTFAIAPLVEDVTATIAPVAAKNGNEISVHCPPNIGEMQSDSTRIRQ
                     ALLNLLSNANKFTEHGRVTVRVSRIMHNGSEAISAAVADTGIGMSR"
     gene            2073..>2263
                     /locus_tag="VM713_06100"
     CDS             2073..>2263
                     /locus_tag="VM713_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83869.1"
                     /translation="MTEPPRSHDEHRHRVGQFEFRMLTELKRRNVFRVAGPYLGSSWF
                     AVHIGSVLGENFTLLHRIMP"
BASE COUNT          369 a          754 c          719 g          421 t
ORIGIN      
        1 cgtgacatgc cgatgccggt atcggcgacc gccgccgaga tcgcttcgct gccgttgtgc
       61 atgatgcgcg acacacggac tgtgacgcga ccgtgctcgg tgaacttgtt agcgttgctg
      121 agcagattga gcagcgcctg gcggatccgc gtcgagtcgc tctgcatctc gccgatgttc
      181 ggcggacagt gcacgctgat ctcgttgccg ttcttcgccg ccaccggggc gatggtggcg
      241 gtcacgtcct cgaccagcgg cgcgatcgca aaggtctgca cgtgcaggtc catcctgccg
      301 gcctcgatct tcgacaggtc cagaatgtcg ttgatgagct cgagcaggtg gtgggccgcg
      361 cgcagcacac gttccagggg ctctacctct tccgtgcggt tcagctcgcg cgcatcctcg
      421 tgcagcattt cggtcaagcc gatgatggca ttgagcggcg tacgcagctc atgcgacatg
      481 ttggcgagga actgcgactt cagtttgctc acttgggcca gctgctcgct cttggcctca
      541 ctttccttga acagtcgcgc gttttcgatt gccaacaccg actgcgacgc cagtgtctgc
      601 agcagtcgca cggtcgaggg cggaaattcg cccttgctct tgcggcggat cagcagggca
      661 ccgagcacgt gatcctcgcg gagcagcggc accgcgatca cgccgtggat gccttcgtac
      721 agccgcagat tcttcaggtt ctggttgaac agctcgacgt cggtcacctg aaccggcgca
      781 cggcggaccg cgcaggtacc gagcggcccg tctcgcaggc cgatacgccg gccacgcagg
      841 ttctctatcg cgtcggggct gaccccgaag ttggcacggg gatcgaatac cccatcggtg
      901 tcgctgaacg agtacagcgt gccggaatcc gcgtccgcga ggtgcacggc tcgctcaatg
      961 acggtagcca gcaccgtttc gagctcgaga gaggagctca ccgcctggcc cacctcgctc
     1021 aaggcctgca gctcggtcac cgaccgcgtc aactcgcgcg tgcgggcctc gacctcggcg
     1081 gtgcgttcgg cgacccgctg ctccaggcgg cgctgatcct ccgctatctc cagctgtgac
     1141 tcgtgcagct tctcgctcat caagcgcagc gaggcgagca ggttaccgag atcgcgtttt
     1201 ggcaagctgc cggcggcggc aaactcgccc gccgcgatgc gcccggccag acccaccgcc
     1261 gcgttgagcg gttcggtaac cccggtgatc atgctgtaga gaaaccacgc cgccaccagt
     1321 agggtgaaca cggtagcaac ggctacgatc ttgctctgac gccgagccga atcgatcgcc
     1381 tggccgatgg ccgcgccgct cgcctgctcg taacccgaat ccagtgtgcc tatcgccgcg
     1441 cggatctgcc tgatctgggg ttggagtcgc agcagctcct cgacgggcat ggttacggag
     1501 atccgacccg tgaggtcctg aagtcctgtg gcgactgcag ctgcctcctc atcctttttg
     1561 tgcagcgcaa cggccgcggc caatgaccgc tgcagcgcct cgtacgcggc ccggccgagg
     1621 ctgccggtgt ccacggcgtc cgcggcgtgc gtcataaagg actcgaagct gtccgtcatg
     1681 gcccgcgaca gcccctggcg agcgacgacg cgctccaccg cagtcgtgcg ccgcaggtcg
     1741 gacaccgcct gatagaacag caccagggtg atcggcgcga tgatgatcag gaactgcgcg
     1801 cagatccaca attttagttg ttcgactttt atcattggga cccagcccgg tccgccccga
     1861 caaccgccgc gcagacctgc cccgctctcg atttctacgc ggtgtgcggc gggccgtcaa
     1921 tcgcgcgccg cccgccgctc ggcgggtgcg tatcgtgacg tggttgcgcc aggtgccccg
     1981 gaggtgaccg tgagacgctc gatgtcaggt tcttgacaga atcgcccccc gcagcggata
     2041 aaactcgccg gcgtggggga gaaaacaccg agatgaccga gccgccgcgc agccacgacg
     2101 agcaccggca tcgagtcggg caattcgagt tccggatgtt gacggagctc aagcgccgca
     2161 acgtgtttcg tgtggccggc ccgtatctgg gctcgtcctg gttcgccgtg cacattggat
     2221 ccgtgctcgg cgagaacttc actttgcttc accgcatcat gcc
//
LOCUS       DATLKL010000458         2255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_219568, whole genome shotgun sequence.
ACCESSION   DATLKL010000458 DATLKL010000000
VERSION     DATLKL010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219568"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..549
                     /locus_tag="VM713_06105"
     CDS             160..549
                     /locus_tag="VM713_06105"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HVH83870.1"
                     /translation="MKKGGSPQDKTPAQLIDARIAELGDWRGKTLSTLRALIKEADPG
                     VVEEWKWSVPVWSHDGLICTGETYKKVVKMTFAKGAALEDPARLFNSSLEGNTRRAID
                     FHEGAPIDQEALKTLIRAAVTLNKSTT"
     gene            593..1630
                     /locus_tag="VM713_06110"
     CDS             593..1630
                     /locus_tag="VM713_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVH83871.1"
                     /translation="MLAATSRGACASAHSGLEIYFIDVEGGQSTLLVTPDRHSLLIDA
                     GWAGDGSGFHPGDPHQARDANRIVAAARDAGVAQIDYLFITHFHVDHDGGVGELAKLM
                     PVRVFIDHGSPSDEAQKTSPETRDGFAAYLKVRGTAAGHLEPRPGDLLPLTDVEAIVV
                     SSAGATLAWSLPGAGEKNPLCRAQAPPPLDPYENPRSTGLVVRYGRFRFLDLGDLTGQ
                     PLFNLACPKNLIGPVDVYLVAHHGGPDAADPATFAAFMPSVAIMNNGLMKGGALTTYE
                     TLHQVPGLENVWQLHLSAEAGERNFAPTYSANLDEGTAYWIKLVANEDGSFRVFNQRT
                     GVWREYAARRR"
     gene            2107..>2255
                     /locus_tag="VM713_06115"
     CDS             2107..>2255
                     /locus_tag="VM713_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVH83872.1"
                     /translation="MTTLTINGKSVEVQAAADTPLLWVLRDHLGMTGTKFGCGAGFCG
                     ACTVHL"
BASE COUNT          399 a          731 c          733 g          392 t
ORIGIN      
        1 cgaaagctta cgcataggct tggagtctat gccggcacgt gcattaaaac actgctctca
       61 tcgaggcccc gcgcgtgctc cgacatggat cgctacggcg gcggcacggc agtatcctgc
      121 gctcaacccc ctggccgccg ccggggagga ggtgccgaca tgaagaaggg cggatcgccg
      181 caagacaaga ctcctgcgca gctgatagac gcgagaatcg cagagctcgg cgactggcgg
      241 ggcaagacgc tcagcacgct ccgcgctctg atcaaggaag ccgatcctgg ggtcgtcgag
      301 gagtggaagt ggagcgttcc ggtgtggtcg cacgacgggt tgatctgcac cggcgagact
      361 tacaagaaag tggtgaagat gacgttcgcc aaaggagccg ctcttgagga tcctgcgcgc
      421 ctgttcaact ccagcctcga ggggaacacg cggcgtgcca tcgacttcca cgagggcgcg
      481 ccgattgacc aggaggcgct caagactctg atccgcgccg ccgtgaccct gaacaagtcg
      541 acgacctgac gctgtggctg ctcggtcgat ttacggtgcg tgcctcctcg cgttgcttgc
      601 ggccaccagc cggggcgcct gcgcgtcggc acattcgggt ctcgagatct acttcatcga
      661 cgtcgaggga gggcaatcaa cactcctcgt cacgccggac cggcattccc tgctgatcga
      721 cgcggggtgg gcgggtgacg ggtccggttt ccatcccggg gacccgcatc aggcgcggga
      781 cgcgaaccgc atcgtcgccg ccgcccgcga cgcgggagtc gcacagatcg actacctctt
      841 tattacccat ttccacgtgg atcatgacgg cggcgtcggc gagcttgcca agctgatgcc
      901 cgttcgagtc ttcatcgatc acggctcgcc cagcgacgag gcccaaaaga ccagtcccga
      961 gacccgggat gggtttgccg cgtatctgaa ggtgcgcggc accgcggccg ggcacctcga
     1021 gccgcggccc ggcgatcttc tcccgctcac ggacgtggaa gccatcgtcg tcagttccgc
     1081 gggcgcaacg ctcgcttggt ctctcccggg cgcgggcgag aagaacccgc tctgccgagc
     1141 ccaggcgccg ccgccgctcg acccttacga gaacccgcgc tcgaccgggc tcgtggtccg
     1201 ctacgggagg ttccgctttc tcgatctggg tgacctgacc ggtcaaccgc tattcaatct
     1261 tgcctgtccg aaaaacctca tcgggcccgt ggatgtttac ctggtggccc atcacggtgg
     1321 tcccgacgcg gcggatcccg cgaccttcgc ggcgttcatg ccgagcgtcg cgatcatgaa
     1381 caacggtctg atgaaaggtg gggcgctcac cacttatgag accctgcatc aggtgcccgg
     1441 actcgagaac gtctggcagt tgcacctgtc cgccgaggca ggcgagcgta atttcgcgcc
     1501 tacctacagc gccaatctcg acgaaggcac ggcctactgg atcaagctcg tcgcgaacga
     1561 ggatggctcc ttccgcgtct tcaatcagcg cactggcgtg tggcgcgagt acgcggcacg
     1621 gcgccggtga cccggcgcag ggccgacgca gccgctactt ctgaatccag aatttgtaca
     1681 tcgcggtaaa ggcgtcggga ttgtcagcct cgaggcgcgc gcggtagtga ctgatccgcc
     1741 gcacggccga gtgcgcctgc gagccggtgc gctgcctgtg cggcagccgc cggggtcaag
     1801 ggaatcgttc cgggatctgg catcagcggt cggggtcgcg agactctaag tcgttgattg
     1861 gcggactctc gagctggacg gctttcgcac cgtggtatcg atcggcgtag ccggcggctg
     1921 agcagcaccc acaccgccgc gctgttactt gcggaagccc tgcgcgcgtt ggattatcac
     1981 gcgggcctcg caacaacaaa ggcatgttta aaggggccgc tcgcccgcaa agcatagaag
     2041 cataccgaac cggcttagtt ccgagcgtcg ccggcttgag cccttacacg gaccgggagt
     2101 ccttccatga ccacactcac catcaacggc aagagcgtcg aggtacaggc agccgcggac
     2161 acgccactcc tatgggtgct gcgcgaccac ctggggatga ccggcacgaa gttcggttgt
     2221 ggcgcggggt tctgcggcgc ctgtaccgtg catct
//
LOCUS       DATLKL010000459         2510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_208383, whole genome shotgun sequence.
ACCESSION   DATLKL010000459 DATLKL010000000
VERSION     DATLKL010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208383"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VM713_06120"
     CDS             <1..389
                     /locus_tag="VM713_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83873.1"
                     /translation="AAYGEGERATLEAAIDTALARATRGAPLPAGLAIKVTVEDVAPS
                     HPTREQLMADPAADPTQTHFLGGAELAGEVRDASGHVLTTVTHRYFPITLALGSSSLD
                     PWADARLAIDQFAVKLAAACRDLSRS"
     gene            complement(349..1515)
                     /locus_tag="VM713_06125"
     CDS             complement(349..1515)
                     /locus_tag="VM713_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVH83874.1"
                     /translation="MPRRLAGRCRVSLRLYFWGRGDRMSSDKAQGRAPGKATSFDIAY
                     RAGVSQPTVSRALRGSPRVSEETRKRVLAVAQQLNYRVDKNASNLRFQRSNTLALLIF
                     EDPTSDDSAINPFFVAMLGSITRACAQRGYDLLVSFQQRSSDWHKEYEDSRKADGLIL
                     LGYGDYLLYRERLERLTEQGTHFVRWGAVQPGQPGLSIGSDNFQGGYEAARHLLSLGR
                     RRLAFLGGVSRGCPEFLDRYRGFGRAIAEVGAPASAALQVDAISTEQAGHAAAGELLA
                     RGVPFDAIVGASDLIALGAMRALQERGLAVPADVAVVGFDDIPAAGLANPPLTTIAQN
                     TRLAGEVLVETVLRRIREEPAEARMLPARLVVRRSCGAQAELSSVTDRGTRPQA"
     gene            1697..>2510
                     /locus_tag="VM713_06130"
     CDS             1697..>2510
                     /locus_tag="VM713_06130"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVH83875.1"
                     /translation="MVQKIRDVSVGVRRWAMEAAAGCGLVLIGVTGPALAQEQAASTT
                     GSAELSLATITITGVRAAVETAINVKEHADNIVEVISAEDIGKLPDTSIAESISRLPG
                     LTSQRADGRASDISIRGTDPQFATGLLNGREQVSTSDNRNIEYDQYPAELISGVVVYK
                     TPDAALIGQGLSGTIALQTIRPLEYGHRATVLDFRLEKNSDASMGADSKNNGYRGSFS
                     FIDQFLDNTLGVALGYARLSSPVVGRELGLYDPWQPNGTEHANLSPTVNVTNG"
BASE COUNT          415 a          861 c          846 g          388 t
ORIGIN      
        1 tggctgccta cggggaaggg gagcgcgcca cgctcgaggc cgcgatcgac acggcgctgg
       61 cgcgcgcgac ccgcggcgcg cccttgcccg cgggactcgc gatcaaagtg accgtcgagg
      121 acgtcgcgcc gtcccatccc accagggagc agctgatggc agacccggcg gcggacccga
      181 cgcagaccca cttcctgggc ggtgcggagc tcgccggcga ggtgcgcgat gcgagcgggc
      241 acgtgttgac caccgtcacc caccgctact ttccgatcac gctcgcgctc ggctcgagct
      301 cactcgaccc gtgggcggat gcgcgcctcg cgatcgatca gttcgcggtc aagcttgcgg
      361 ccgcgtgccg cgatctgtca cggagctgag ctcggcctgc gctccgcacg agcggcggac
      421 gacgagtcgc gccgggagca tccgtgcctc ggcgggctcc tccctgatcc ggcgcagcac
      481 agtctcgacc agcacctcgc ccgcgaggcg ggtgttctgc gcgatggtcg tcagcggcgg
      541 gttggcgagc ccggcggccg ggatgtcatc gaagccgact accgcaacgt ccgcgggcac
      601 cgcgagcccg cgctcctgca gcgcgcgcat ggcgccgagc gcgatgaggt cgctcgcgcc
      661 cacgatggcg tcgaacggca cgccgcgcgc gagcagctcg ccggccgcag cgtggcccgc
      721 ctgctcggtg ctgatggcat cgacctgcag tgccgcgctc gccggagcgc cgacttcggc
      781 aattgcccgc ccgaagccgc ggtagcgatc gagaaactcc gggcaacccc gtgagacccc
      841 gccgagaaag gccaggcggc ggcggccgag gctgagcaga tgccgggccg cttcgtagcc
      901 tccctggaag ttgtccgatc cgatcgacaa ccccggctgt cccggctgca cggcgcccca
      961 gcgcacgaaa tgagtcccct gctcggtcag gcgctcgagc cgctcgcgat agagcaggta
     1021 gtcgccgtag ccgagcagga tcaggccgtc ggctttgcgg ctgtcctcgt attccttgtg
     1081 ccagtcgctc gagcgctgct ggaacgacac gagcagatcg tagccgcgtt gtgcgcaggc
     1141 gcgggtgatc gagccgagca tcgcgacgaa gaacggattg atcgcggagt catccgaggt
     1201 cgggtcctcg aagatgagga gcgcgagggt gttggagcgc tgaaagcgca ggttggaggc
     1261 gttcttgtcg actcgatagt tgagctgctg tgcgacggcg agcacgcgtt tgcgggtctc
     1321 ctcgctgacg cgcgggctgc cgcgcaacgc acgcgatacc gttggctggg acaccccggc
     1381 ccggtacgca atgtcaaacg acgtggcctt acccggcgca cgtccttgag ccttgtcgct
     1441 gctcattcgg tccccccgcc cccaaaagta taggcgtaaa gaaacccggc agcgacccgc
     1501 aagtcgccga ggcatcgcca agggtgggcc ggagcgggcg ctgttgcata tgaatacgta
     1561 tgccggctct tccggtcggt tgcgcccgat cgctagagtc aagcaacgct cgctagcggg
     1621 cgctcaagta tgcggtggtg cgcggatcct gcgggttcgc ggaccggccg tgtaccacca
     1681 ctcgggggga ttcactgtgg ttcaaaagat acgagacgtg tcggtcggcg tgcggcgctg
     1741 ggcgatggaa gctgctgcgg gctgcggcct ggtgttgatc ggtgtgaccg gccccgctct
     1801 ggctcaggag caggcggcat cgactaccgg ctctgccgaa ctgtcattgg cgacaatcac
     1861 catcacgggc gtccgcgccg cggtcgagac cgccatcaac gtcaaagagc acgctgacaa
     1921 catcgtcgaa gtgatctccg ctgaggacat cggcaagctg ccagacacga gcatcgcgga
     1981 gtccatctcg cgcctgcccg gtctcacttc acagcgcgcg gacggacgcg cctcggacat
     2041 cagcatccgc ggcaccgatc cgcagttcgc cacggggctt ctcaacggcc gcgagcaggt
     2101 gagcaccagc gacaaccgta acatcgagta cgaccagtac ccggcggagc tgatcagcgg
     2161 cgtggtcgtc tacaagaccc ccgacgcggc gctcatcggc caggggctct ctggcacgat
     2221 cgcgctgcag acgatccgcc cgctcgagta cggccaccgc gccaccgtgc tggacttccg
     2281 tctggagaag aactccgacg cttccatggg cgccgactcc aagaacaacg gttatcgcgg
     2341 cagcttctcc ttcatcgacc aattcctcga caacactttg ggtgtcgccc tgggatatgc
     2401 gcgcctctcc tcgccggtcg tcggccggga gctcgggcta tatgacccgt ggcagccgaa
     2461 cggtaccgag catgccaacc tgtccccgac cgtcaacgtg accaacggca
//
LOCUS       DATLKL010000460         2732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_208386, whole genome shotgun sequence.
ACCESSION   DATLKL010000460 DATLKL010000000
VERSION     DATLKL010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208386"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VM713_06135"
     CDS             <1..979
                     /locus_tag="VM713_06135"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HVH83876.1"
                     /translation="TIEQIDLVKQMCARYPQDLAMASTAADIVRLHKSGRIAALIGVE
                     GGHQINNSLAALRAYYELGARYMTLTHSANIAWVDSATDNPAHHGLTPFGKEVVREMN
                     RLGMLVDLSHVSADTMRAALSVSEAPVMFSHSSARALVDHPRNLPDDVLRLVAARGGV
                     VMVNFYPGYVSEPRRRWEADRAAEQARFNSPPFGGLYIGQPERAKAALEEWEHAHPKP
                     AVGIGAVADHIEYVARVAGIDHVGLGSDFDGMPEAPAGLDGVDKYPALLAELAHRGLS
                     DADLGKVAGGNLLRVLAKAEEVSARLRTTRGPSTATLAQLDGPPGAATH"
     gene            complement(1023..1940)
                     /locus_tag="VM713_06140"
     CDS             complement(1023..1940)
                     /locus_tag="VM713_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HVH83877.1"
                     /translation="MSETTRRAFLREAGRCSLAMLAAQAAPGVLRATPLGGPVGIQLY
                     AVRDTLRAEPAATLKALRAIGYGEVEAAGFAGLSVRRFRALLDEAGLGCPSAHLAFDP
                     DNLGAAFDDAHALGARYAASGSLRALVQRDLPEAAAADSGMSLEEARHTAALANHIGE
                     RAARVGLQYVYHNHDFEFADQGGGAIGYDLLLKETDPRLVKFEIDCGWMTLGGRDPRK
                     YFARYPRRFPMIHVKDFLRSAAGPRGPGAPAAAGAELGHGMIDYRPILAAAEKAGLKH
                     YFAEQEGPFTRMTELEAARQAYQYLSRLP"
     gene            complement(1937..>2732)
                     /locus_tag="VM713_06145"
     CDS             complement(1937..>2732)
                     /locus_tag="VM713_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HVH83878.1"
                     /translation="FVDLTDATALEAALRPNTRLVWVETPTNPLLKVVDLTALASLAR
                     KHRLVSVCDNTFATPYIQRPLEHDFDVVVHSTTKYLNGHSDSIGGAAVVRAGSELTER
                     LGFLQNAVGGVSSPFDSFLTLRGIKTLALRMERHCANALAVAKFLEQHAAVERVYYPG
                     LASHPQHALARRQMQGGYGGIVTAVLRGGLASARRTLERCRLFALAESLGGVESLIEH
                     PGIMTHASLPEATRKVLGINDGLIRLSVGVEDLQDLIEELERALSS"
BASE COUNT          424 a          969 c          937 g          402 t
ORIGIN      
        1 gaccatcgag cagatcgatc tcgtgaagca gatgtgcgcc cgctatcccc aggacctcgc
       61 catggcgtcc acggccgcgg acatcgtgcg gctgcacaag tccggccgga tcgccgcgct
      121 catcggcgtc gagggcggcc atcagatcaa caactccctg gcggcgctgc gcgcgtacta
      181 cgagctcggc gcgcgctata tgactctcac gcactccgcc aacatcgcgt gggtcgactc
      241 ggccaccgac aatcccgcgc accacggact cacgcccttc ggcaaggagg tcgtccgtga
      301 gatgaaccgc ctcggcatgt tggtcgacct gtcgcacgtg tcagcggaca ccatgcgcgc
      361 ggcgctcagt gtcagcgagg cgccggtgat gttctcgcat tcctcggcac gcgcactcgt
      421 tgatcaccca cgcaacctgc ccgatgacgt cctgcgcctg gttgcggcca gaggcggcgt
      481 cgtaatggtg aacttctacc cgggctacgt ctccgagcca cgccgccgct gggaagccga
      541 ccgcgccgcc gagcaggcgc gcttcaacag cccgccattc ggcgggctgt acatcgggca
      601 acccgagcgc gccaaggcgg cactcgagga atgggagcac gcgcacccca agcccgcggt
      661 cggcatcggc gcagtagccg atcacatcga gtacgtcgcc cgggtcgccg gcatcgatca
      721 cgtgggcctc ggctcggact tcgacggcat gcccgaggca cccgcgggtc tcgacggcgt
      781 cgacaagtat cccgcgctcc tcgccgagct cgcccaccgc ggcttaagcg atgcggatct
      841 cggcaaggtc gccggcggca acctgctgcg cgtgcttgcg aaagcggaag aggtcagcgc
      901 gcgactgcgc acgacgcgcg gcccctcgac ggcgacgctg gcgcagctcg acgggccgcc
      961 aggcgcggcc acgcactgat tttgccgccg gcgaaaggcg gggctcgata cgcccgtacc
     1021 gactaaggca ggcgactcag gtactggtac gcctgacgcg cggcctcgag ctcggtcatg
     1081 cgcgtgaagg gtccctcctg ctcggcgaag tagtgcttga ggccggcctt ctccgcggca
     1141 gcgaggatcg gccggtagtc gatcatgccg tggccgagct cggcccccgc agcagccggc
     1201 gcgccgggcc cgcgtggccc cgcggcgctg cgcaggaagt ccttcacgtg gatcatcggg
     1261 aagcgccgcg ggtagcgcgc gaagtacttg cgcggatcgc ggccgcccaa tgtcatccag
     1321 ccgcagtcga tctcgaactt gacgagccgc gggtcggtct ccttgagcag caggtcgtag
     1381 ccgatggcac ctccgccctg atccgcgaac tcgaagtcgt gattgtgata gacgtactgc
     1441 agccccacac gcgcggcacg ctcgccgatg tgattggcaa gcgcggcggt gtgccgtgcc
     1501 tcctcgaggc tcatgccgga gtcagccgcg gcggcctccg gcaggtcgcg ttgtaccagc
     1561 gcgcgcaggc tcccgctcgc cgcatagcgg gcgccgagtg cgtgcgcgtc gtcgaacgcc
     1621 gcacccaggt tatccgggtc gaacgcgaga tgtgcgctcg gacagccgag cccggcctca
     1681 tcgagcagcg cgcggaaccg ccgtacgctc aagcccgcga agccggcggc ctccacttcg
     1741 ccgtagccga tggcgcgcag cgccttgagc gtcgccgcgg gctcggcccg cagcgtgtcc
     1801 ctgaccgcat agagctggat cccgaccggg ccgccgagcg gcgtcgcgcg cagcacgccg
     1861 ggtgcggcct gagcggcgag catggccagg ctgcagcggc ccgcctcgcg caggaaggct
     1921 cgccgcgtcg tctcactcac gagctcaacg ctctctcgag ctcctcgatc agatcctgca
     1981 ggtcctcgac cccgaccgac aggcggatca gcccgtcgtt gatgccgagc accttgcgcg
     2041 tcgcctcggg aagcgaggcg tgagtcatga tgcccggatg ctcgatgaga ctctcgacgc
     2101 cgccgaggct ctcggcaagc gcgaacaggc ggcagcgctc gagcgtgcgg cgcgccgaag
     2161 cgagtccgcc gcgcagcacc gccgtcacga tcccgccgta gccgccctgc atctgccgcc
     2221 gggcgagcgc gtgctgcggg tgactggcga gacctgggta gtacacccgc tccaccgccg
     2281 cgtgctgctc gaggaacttc gcgaccgcga gcgcgttggc gcagtgccgc tccatgcgca
     2341 gggcgagtgt cttgatgccg cgcagcgtga ggaagctgtc gaacgggctc gacacgccac
     2401 cgaccgcgtt ctgcagaaag ccgaggcgct cggtgagctc gctcccggcg cgcaccacgg
     2461 cggcaccgcc gatcgagtcc gagtgcccgt tcaggtattt ggtggtcgag tgcaccacga
     2521 catcgaagtc atgctcgagc gggcgctgga tgtagggagt cgcgaaagtg ttgtcgcaga
     2581 cgctgaccag ccgatgtttt ctcgccagcg acgcgagcgc ggtcaggtcc acgaccttga
     2641 gtagcgggtt ggtgggtgtc tccacccaga cgaggcgcgt gttgggccgc agcgcggcct
     2701 cgagcgccgt ggcgtcggtg aggtcgacga ac
//
LOCUS       DATLKL010000461         2864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_185974, whole genome shotgun sequence.
ACCESSION   DATLKL010000461 DATLKL010000000
VERSION     DATLKL010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185974"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VM713_06150"
     CDS             complement(<1..238)
                     /locus_tag="VM713_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVH83879.1"
                     /translation="MARATADIGTINYTTALVAGHHPLTADEGAALGGKDAGPAPHEL
                     LCAALAACTATTLRMYAERKTWPLQAAHVDVLFQH"
     gene            318..866
                     /locus_tag="VM713_06155"
     CDS             318..866
                     /locus_tag="VM713_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HVH83880.1"
                     /translation="MPEHPLPGADLAVPVEASDHTLGPEHAPVVVVEYGDFECPTCKQ
                     AAPSVRLLLERFASRVRVAFRHFPLEQAHPHALAAAEAAESAAEQGKFWEMHDLLFAN
                     QEHLAARHLNSYAERLGLDMARFTAEMDDHVYLQRIREQQDGARRSHVRSTPGFFVNG
                     RIQDVSFGFERLFDTVSASLRR"
     gene            complement(886..2007)
                     /locus_tag="VM713_06160"
     CDS             complement(886..2007)
                     /locus_tag="VM713_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVH83881.1"
                     /translation="MRSLGRRPEGNRLERIAASPRWGGGGFRNLHPILPGLRDPTAAM
                     PTLREFLCGGEHRFPPGPLPSVSPLDTWSKPPGSGLRVTWLGHSTVLIEIGGLRVLTD
                     PVWGPRASPSRFIGPRRFQPVPVALRSLPPIDLVLVSHDHYDHLDYPTIRQLRKHPVP
                     FVTSLGVGAHLEAWGVEPERIVELDWWESYSLPNADLTVTAAPSQHFSGRTLKTRNTT
                     LWSSLVVRSGGHAVFFSGDTGLTSEYQQIRARLGPFDLTMLEVGAWHPAWGDMHLGPE
                     NALKAHALLGGGAFLPIHWGTFALAMHPWDQPAETLLRLGPAAGAQLLMPRLGEPLEP
                     AHGRDVEPWWRTERPERAVPLRETLTAAAVPKSIAVPMD"
     gene            2149..2814
                     /locus_tag="VM713_06165"
     CDS             2149..2814
                     /locus_tag="VM713_06165"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HVH83882.1"
                     /translation="MERTSRRSALPLIWIAWLWLGACAIGAGRAEAQSLCESCEVQLG
                     LGATYHFWGPTGGVVLPVTVNWSDQRYELGLFRVTTQQKLTDHAYPEGRIMADPYWGM
                     SLARRWRLIERGRVRGFVGFGLAVKTESDQLSSTRIDFASQLGMRFRLPGDRVTGELT
                     LRHWSNAGIRLPNHGQDFATLTFRLNSGLFGVPRADVIPLDPSFDLARAAWLGSRDGE
                     ALP"
BASE COUNT          434 a          991 c          983 g          456 t
ORIGIN      
        1 cgtgctggaa cagcacgtcg acatgcgccg cctgcagcgg ccaggttttg cgctcggcgt
       61 acatgcgcag cgtggtcgcg gtgcaggcag cgagcgccgc gcacagcaac tcgtgcggag
      121 cgggacctgc gtccttaccc ccgagcgccg cgccttcgtc ggcggtcagc gggtgatgcc
      181 ccgccaccag tgcggtcgtg tagttgatgg tgccgatgtc cgccgttgca cgcgccatgc
      241 tggttctcct ccggccgcgg gagcctccgc aagtctggca ggcgacgcgc tagaatgcga
      301 ttctcgagga gtctgcaatg cccgagcatc cccttcctgg cgcggatctg gccgtgcccg
      361 tcgaagcttc cgaccacacg ctcggccccg agcacgcgcc ggtcgtcgtg gtcgagtacg
      421 gcgacttcga gtgtccgacc tgcaagcagg ctgcgccgag cgtgcggctg ctgctcgagc
      481 gtttcgcgag ccgcgtgcgc gtcgctttcc gtcattttcc gctcgagcag gctcaccccc
      541 acgcgctggc agcggccgag gccgccgaga gtgccgccga gcagggcaag ttctgggaga
      601 tgcacgacct gctgttcgcc aaccaggagc atctggcggc caggcacctc aactcctatg
      661 ccgagcgcct cggactcgac atggcgcgct tcaccgcgga gatggacgat cacgtctacc
      721 tgcagcgcat ccgcgagcag caggacggcg cacggcgcag ccacgtgcgc agcacgcccg
      781 gctttttcgt caacgggcgg attcaggatg tgtcgttcgg cttcgagcgg ctgttcgaca
      841 ccgtgagcgc gtcgctacga cgatgacggc agcgcccggc accggctagt ccatcggaac
      901 cgcgatcgac ttcggaaccg cggcggcggt cagcgtctcg cgcagcggca ccgcccgctc
      961 cggccgctca gtgcgccacc agggctcgac gtccctgcca tgggccggct cgagcggctc
     1021 gccgaggcgc ggcatgagca gctgcgcccc ggccgccggt ccgagcctca gcagggtctc
     1081 ggcgggctgg tcccacggat gcatcgccag ggcgaacgtg ccccagtgaa tcggcaggaa
     1141 tgccccgcct ccgagaagcg catgcgcctt gagcgcgttc tcgggaccta ggtgcatgtc
     1201 gccccaggcg ggatgccacg cgcccacctc gagcatggtg aggtcgaagg gcccgagccg
     1261 cgcgcggatc tgctgatact cgctcgtgag tccggtgtca ccgctgaaga acaccgcgtg
     1321 cccgcctgag cgcacgacca gcgaggacca cagcgtcgta ttacgcgtct tgagcgtgcg
     1381 cccggagaag tgctgcgaag gcgcggcggt gaccgtgaga tcggcgtttg gcagcgaata
     1441 ggactcccac cagtcgagct cgacgatgcg ctcgggttct acgccccagg cctcgagatg
     1501 cgccccgacg ccgagtgagg tcacgaacgg caccgggtgc ttgcgcagct gccggatggt
     1561 cggatagtcg aggtgatcgt agtgatcgtg cgagacaagg acgagatcga tgggtgggag
     1621 cgagcgcagc gccaccggca cgggctggaa ccgcctcggc ccgatgaacc gcgaaggcga
     1681 ggcgcgcggt ccccacaccg ggtcggtgag gacgcgcagc ccgccgatct cgatgagcac
     1741 cgtcgagtga ccgagccagg tgacgcgcaa accgctgccg ggcggcttcg accaggtgtc
     1801 cagtggactc accgaaggca gcggccccgg agggaaccgg tgctcgccgc cgcataggaa
     1861 ttcccgcaac gtcggcatcg ccgccgtcgg atcgcgcagg ccaggcagta tcgggtgcag
     1921 gttgcggaag ccgccgcccc cccagcgggg cgaggccgcg atgcgctcga gccggttgcc
     1981 ttcgggtctt cgtcccaggg atctcatctg cctccatcat acttggcgtt cgctcgcgcc
     2041 ggtgctggct ctccacagag agacggccga gggcgcaccg ttagtgcaga gccgccctct
     2101 ccaatgtggc tgatgtgcct cgcccatgtc ccggcctagc gtcaccgcgt ggaacggacc
     2161 tcccgccgca gcgccctgcc gctcatctgg atcgcctggc tctggctggg cgcctgtgcg
     2221 atcggcgccg ggcgcgccga agcccaatcg ctctgcgaaa gctgcgaggt gcagctcggt
     2281 cttggcgcga cttatcactt ctggggcccg accggcggag tcgttctccc cgttaccgtg
     2341 aactggagcg accagcgtta cgagctcggc ctcttccgcg tcaccaccca gcagaagctc
     2401 accgaccacg cctatcccga gggacgcatc atggccgatc cttactgggg aatgtcgctg
     2461 gcgcggcgct ggcggctcat cgagcggggt cgggtgagag gcttcgtcgg cttcgggctc
     2521 gcggtcaaga ccgagtccga ccagctcagc tcgacgcgga tcgatttcgc ctcgcagctc
     2581 gggatgcgct tccggctgcc cggcgaccgt gtgacgggtg agctgacgct gcggcattgg
     2641 tcgaatgcgg ggatccggct ccccaaccac ggtcaagact ttgcgacgct tacgttccgg
     2701 ctcaactcgg ggctcttcgg cgtcccgcgg gcggatgtga ttccgctcga tccgtcgttc
     2761 gatctcgcgc gcgccgcctg gctcggcagc cgagacggcg aagctcttcc atgatggaat
     2821 aggagcccat gaacaccccc gccgcgagcg actcaggggt cgcg
//
LOCUS       DATLKL010000462         1868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_174526, whole genome shotgun sequence.
ACCESSION   DATLKL010000462 DATLKL010000000
VERSION     DATLKL010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174526"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VM713_06170"
     CDS             complement(<1..334)
                     /locus_tag="VM713_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HVH83883.1"
                     /translation="MKALARLLSEELRPKAIRDELAELNAAFVRLDALARIERALAVL
                     PGAYALASSFGAQAAVMLHLVTRSQPDIPVILIDTGYLFAETYRFVDELTERLRINLK
                     VYRSQPSPA"
     gene            complement(443..1429)
                     /locus_tag="VM713_06175"
     CDS             complement(443..1429)
                     /locus_tag="VM713_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HVH83884.1"
                     /translation="MIDKALLDEAWKTVRGAVRWTPLLHSDYLDSVVGARVFLKAESL
                     QIGGSFKMRGAYFRVSRLSPEQRSRGVVAFSSGNFAQALALAGRRTGVPVTIVMPSDA
                     PQRKIELTRRAGATVVLTDHGDRNREEVASERARAIAAEHQYALLHPFDDPLVVAGQS
                     TLMREVLVDARREHVHIDVILCPVGGGGLIAGTALAEKHFGQGAEVIAVEPDACDDMR
                     RSLISHRRERNVGTPQTICDAMQAVSPGAVPFEAAEHLISRGIAVDDAAVRRAMRIAF
                     EEFKIVLEPSGAIALAAALERREEFANRTVVLLCCGGSVSVEDLVRLTSSTH"
     gene            complement(1484..>1868)
                     /locus_tag="VM713_06180"
     CDS             complement(1484..>1868)
                     /locus_tag="VM713_06180"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1275 family protein"
                     /protein_id="HVH83885.1"
                     /translation="WQGPFEDPDKGWALLVGMTLVAAMAMQNAAHRAHLAHTPPSTIM
                     TGTTTQIMLDLAELLHGARPEVAAVTRARLTRLTLSVALFATGCGLASLTYIHAGKWC
                     FSVLPVLGVWTLTLRREIAEADAHK"
BASE COUNT          325 a          604 c          644 g          295 t
ORIGIN      
        1 aggcggggga gggctgtgaa cggtagacct tgaggttgat gcgcagccgc tcggtgagct
       61 catcaacgaa gcgataggtt tccgcgaata ggtagccggt atcgatgagg atcaccggaa
      121 tgtcaggctg ggagcgcgtc acgagatgca gcatgactgc cgcctgagcc ccgaaactgg
      181 aggcgagcgc gtaggccccg ggaagaacgg caagcgcgcg ctcgatgcgc gccaacgcat
      241 cgaggcggac aaacgccgcg ttcagttccg cgagttcatc gcggattgcc ttggggcgaa
      301 gctcctcgga gagcagccgg gccagcgctt tcatctttcg cggtgggatc agtgcatcgc
      361 tctagcacgc agcctaggtc aaggacccgt tccggtaaaa gcacatctgc ttatgtcggc
      421 ataactgtcc ggcacaggat gactagtgcg tgctcgaggt gaggcgcacg aggtcttcga
      481 ccgacacgct gccgccgcag cacagcagga ccacggtgcg gttggcgaat tcctcgcgcc
      541 gctcgagagc cgctgccagc gcgatcgcgc cgctcggctc gagaacgatc ttgaattcct
      601 cgaacgcgat gcgcatcgcc cggcgcacgg cggcgtcatc gaccgctatg ccgcgggaga
      661 tcaggtgctc ggccgcctcg aacggcaccg cgccggggct tacggcctgc atcgcgtcgc
      721 agatggtttg tggcgtgccg acgttgcgct cgcgccggtg cgagatcagc gagcggcgca
      781 tgtcatcgca ggcgtccggc tccactgcaa tcacttcggc accctggccg aaatgcttct
      841 cggcgagcgc ggtgccggcg atcagcccgc cgccgcccac cggacagagg atgacgtcga
      901 tgtgcacgtg ctcgcgacgc gcatcgacca gcacctcgcg catcagggtc gattgccccg
      961 cgacgaccag cggatcgtcg aacggatgga gcagggcata ttggtgctct gcggcaatcg
     1021 cgcgggcgcg ctcgctggcg acttcctcgc ggttgcgatc gccgtggtca gtgagcacga
     1081 ccgtcgcacc ggcacgtcgc gtgagctcga tcttgcgctg tggcgcatcg ctcggcatga
     1141 cgatcgtgac cggcaccccg gtgcggcgac cggcgagcgc gagcgcctgc gcaaagttgc
     1201 ccgaggaaaa ggccacgacg ccgcggctgc gctgctcggg cgagaggcgg ctgacgcgaa
     1261 agtaggcacc gcgcatcttg aacgagccgc cgatctgcag cgattcggcc ttgagaaaca
     1321 cccgcgcacc gaccacggag tccagatagt cggagtgcag caacggcgtc cagcgcacgg
     1381 cgccgcggac ggttttccat gcctcatcca atagagcctt gtcgatcatg agcacctctc
     1441 gacgggaacc ctcgcacgac gtggcctgcg gttcaagcca acgtcattta tgggcgtcgg
     1501 cttccgcgat ttccctgcgc aacgtcagcg tccacactcc gaggacgggc agcacactga
     1561 agcaccactt gcccgcgtga atgtaggtga gcgacgccag gccgcagccg gtcgcgaaca
     1621 gcgcgacgct gagggtgagg cgcgtgaggc gcgcccgggt gacggccgcc acctcgggtc
     1681 gcgctccgtg cagcagctcg gcgagatcga gcatgatctg cgtggtcgtg ccggtcatga
     1741 tggtcgaggg gggcgtgtgg gcgaggtgcg cgcggtgcgc ggcgttctgc atggccattg
     1801 ccgccacgag cgtcatgccg acgagcagcg cccacccctt gtcgggatcc tcgaagggac
     1861 cctgccac
//
LOCUS       DATLKL010000463         2368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_152313, whole genome shotgun sequence.
ACCESSION   DATLKL010000463 DATLKL010000000
VERSION     DATLKL010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152313"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="VM713_06185"
     CDS             <1..1611
                     /locus_tag="VM713_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVH83886.1"
                     /translation="IWVGVVFVLLQVSSPAHHAVGANLGSRTAAWLYDRLTTACVRPP
                     GLGHLEDPRLTTDLTVARDFDLGITGPPLATSMDFIAAELVATLGGLASAVVLAAYSW
                     WAPVVLAGAWLGTHWLLRESAVWRDRNTDEVRQAQRHADYAYRLAVDPPAAKEVRLFG
                     LADWVVERFTSRRRRLLDLQWQATRLRERPVLWSLLIVLAANIALFWAMAEDAATGRL
                     PLARVVTFATAALTTSMIAFGGLSWALDGAAAPVAAVLRLEGAMEPVGALVPGARSAE
                     NMPTREIRFRDLRFAYSQSHKPVLENFNLRIQAGSSLAIVGQNGAGKTTLAKLLCRLY
                     DPQEGAVEIDGVDLKALDVDRWRARLTAVFQDFTRFELSLRDNVAPSGAPDREIRAAL
                     EEAGAADLASLDTVLSRAYAGGTDLSGGQWQRIALARTLCAVRQGAGVVLLDEPTAQL
                     DVRGEAEIFERLLAATRHTTTILISHRFSTVRHADRICVLEHGRAVELGTHDELMAAG
                     GRYRTMFDLQASRFGGEEEEGVVLDALS"
     gene            1637..>2368
                     /locus_tag="VM713_06190"
     CDS             1637..>2368
                     /locus_tag="VM713_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83887.1"
                     /translation="MLRALRRAHQAEPRLLAVSFTLALLAALPDALLALWMKLLADGV
                     LERRHGLVIGAAVGLGASAAATWFLKVLSDRTQRRFRDRVAVALESHVANLQASVASI
                     AHHERPEYLDRLAMLRDQVFVLDHMYMSVFATCGWILRLGVTIALLVSIHPALALLAV
                     FALPTVLTSTWRPGVERVVEERVASMNRLARHLFTVATTAAPGKEVRVTRIGDRLFRQ
                     RRETWERWYGPVARSRWASALWHTLG"
BASE COUNT          338 a          805 c          773 g          452 t
ORIGIN      
        1 atctgggtgg gcgtcgtgtt cgttctcctc caggtgtcgt cacctgcgca tcatgcggtg
       61 ggggcaaacc tgggcagccg tactgcggcc tggctctacg atcgactgac gaccgcgtgc
      121 gtccggccgc ccggactggg acatctcgag gacccccggc tcacgaccga cctgaccgtg
      181 gcacgcgact tcgacctggg catcaccggt ccaccgctag ccacctcgat ggacttcata
      241 gccgccgagc tagtcgcgac gctaggtggt ctggcgtcgg cggtcgtgct cgcggcctat
      301 tcctggtggg cgcccgtggt gctcgccggg gcctggctgg gcacgcattg gctcctgcgg
      361 gaaagcgccg tgtggcgcga ccgcaatacc gatgaagtgc gccaggcgca acgccacgcc
      421 gactatgcct accggctggc ggtcgatccg cccgccgcca aggaagtccg cttgttcgga
      481 ttggccgatt gggtggtcga gcgcttcacg tcgcgtcgcc gtcggctgct cgatctgcaa
      541 tggcaggcca cgcggttgcg cgaacgtccg gtcttgtgga gcttgctcat cgtgctcgct
      601 gcaaacattg cgctgttctg ggcgatggcc gaagatgcag ccaccggccg cttgcctctc
      661 gcgcgcgtcg tcaccttcgc cacggccgcg ctcacgacca gcatgattgc ctttggcgga
      721 ttatcctggg ctctcgacgg ggctgccgcc ccggtcgcgg ccgtcctccg cctcgagggg
      781 gccatggaac ccgtaggcgc cctggttccc ggtgcgcgct cagccgagaa catgccgacg
      841 cgagagatcc ggttccgcga tctccgcttc gcgtactctc aaagccacaa acccgtgctc
      901 gagaatttca atttgaggat ccaggcgggt tcgtcgctcg cgatcgtcgg ccagaatggg
      961 gccggcaaaa cgacattggc gaaactgtta tgtcgcctgt acgacccgca agagggagcc
     1021 gtcgaaatcg acggcgtcga tctaaaagcc ctggacgtcg atcgctggcg cgcccgcttg
     1081 acggcggtgt tccaagattt cacacgcttc gagctctccc tgcgcgacaa cgtagcgcct
     1141 tcgggcgccc cggaccgaga gatccgtgcc gctctcgaag aggccggcgc tgcggatctc
     1201 gcgagcctcg acaccgtact ctcgcgcgcg tacgctggcg gaaccgacct atccggcggc
     1261 caatggcagc gcatcgccct cgcgcgcact ttgtgcgccg tgcggcaggg cgcgggtgtg
     1321 gtgttgctcg atgagccgac tgcgcagctg gatgtgcgcg gcgaagcgga gatcttcgag
     1381 cgtctcctcg ctgccacgcg ccacaccacc accatcctga tctcgcaccg cttctcgaca
     1441 gtgcgtcacg ccgaccgcat ctgcgttctc gaacacggtc gcgcggtcga actgggcacg
     1501 catgacgaat tgatggctgc cggcggccgc tatcgcacca tgttcgattt acaggcctcg
     1561 cgtttcggcg gcgaggaaga agagggagtg gttcttgatg ccctctcctg acgctctgcc
     1621 agccggcctt tcttcgatgc tgcgcgcgct tagacgcgcc caccaggccg aaccgcggtt
     1681 gctagcggtc tcattcacgc tggcgctgct tgccgccttg ccggacgcgc tgctcgcgtt
     1741 gtggatgaag ctgctggccg atggcgtgct cgagcggcgg catggcctcg tgattggcgc
     1801 agccgtcgga ctcggcgcct ccgccgccgc cacctggttt ctcaaagtgt tgagcgaccg
     1861 cacgcagcgc cggttccgcg accgggtcgc ggtggcattg gaatcccatg tggcgaacct
     1921 gcaagcctcg gtcgcgagca tcgcgcacca cgagcgtcca gagtacctcg accggctggc
     1981 catgctgcgc gaccaggtct tcgtactcga tcacatgtac atgtcggtgt tcgcaacttg
     2041 cggctggatc cttcggctcg gcgttacgat tgccctgttg gtctcgattc accccgcgct
     2101 cgccctgctg gcggtttttg ctttaccgac cgttctgacg tccacgtggc gtccgggtgt
     2161 ggagcgcgtc gtcgaggagc gggttgcctc gatgaaccgg ttggcgcgcc atctgttcac
     2221 cgtcgccacc acagcggcgc ccggcaagga ggtgcgggtc acgcggatcg gcgaccgtct
     2281 cttccggcaa cgccgcgaga cgtgggagcg ctggtacggc cccgtcgccc gctcccgctg
     2341 ggcgagcgcc ctctggcata cacttgga
//
LOCUS       DATLKL010000464         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_141047, whole genome shotgun sequence.
ACCESSION   DATLKL010000464 DATLKL010000000
VERSION     DATLKL010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141047"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VM713_06195"
     CDS             <1..182
                     /locus_tag="VM713_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83888.1"
                     /translation="ALRTQGGDDILVICGGVVPPQDYEILLAKGVSAIYGPGTNIPLA
                     AGEILSLIRGHRKAA"
     gene            262..1797
                     /locus_tag="VM713_06200"
     CDS             262..1797
                     /locus_tag="VM713_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83889.1"
                     /translation="MPKSGPASSAAGAVQLFLLFLGILVTIGWFAFTGAYITGRLGWP
                     ALLQVPPSQLGELLAGMFAPPAFLWMLLAYIARGAEFRREATRVAAYLETLTYPAEGA
                     GARVREVTVALRAQAQELNDATARAAAAALELRQALAAQGQELVSAAGDLVSRAAAAR
                     AALGNDAREFSELAGRIATGVEKLEEALARERQILGDTLTEAETRGQTLNGVLHAQTA
                     LIADAASRAAESTTSLEQALARNTDTLAAAATRADEQGAAIDERLAAARTALDQTGLR
                     AVERAGEIEAHLRERAEELARTADGAASTAVVIGEALATHTKQIAAAGAKAHREAEGL
                     REGLAGQTRALEELTSALAERAKTIEEALSRQAERLERASRPVLRLAEAERKVRREGF
                     LEASKAVREALGSIAVDLNRALQRELPRDAMQRYQAGDRGIFVRNILGWRDGALARAI
                     KDKYGSDNEFRRAVDSYIVKFQELLSGADAADGDALLAATFLTSDVGKLYMVLARAIG
                     RLK"
     gene            1876..>2339
                     /locus_tag="VM713_06205"
     CDS             1876..>2339
                     /locus_tag="VM713_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HVH83890.1"
                     /translation="MTPAEPTTLPRGTARRGPATVRALLPYLWPKGAPELRTRVVIAL
                     LFLVGAKLCNVYVPILYKRAVDQLSAPRPGAAALALGVPLTLILGYGAARVLTLAFAE
                     LRDAVFAKVAQRAIRRAALATFEHLHALSLRFHLERQTGGLSRAIERGTAGIA"
BASE COUNT          326 a          851 c          829 g          333 t
ORIGIN      
        1 aggcgctgag gacccaaggc ggagacgaca tcctggtcat ttgcggcggc gtggtgccgc
       61 cccaggacta cgagattctg ctcgccaagg gggtgagcgc gatatacggt ccgggcacca
      121 acattccact ggccgcgggc gagatcctct cgctgatccg cgggcacaga aaggcagcgt
      181 gacgggttgg cgcacagtct gagggaagag ctgcagggca gcggtgcagc gcccgcgacg
      241 ggccggcggg acttggtgac cgtgccgaag agcggcccgg cgtcgagcgc ggcgggggcc
      301 gttcagcttt tcctcctttt cctcggcatc ctggtcacca tcggatggtt cgccttcacc
      361 ggcgcctaca tcaccggacg gcttggctgg ccggccctgc tgcaagtccc gcccagccag
      421 ctcggcgagc tcctcgccgg catgttcgcg ccgccggcat tcctttggat gctgctcgcc
      481 tatattgcgc gcggcgccga gttccggcgc gaggcgaccc gggtggctgc ctatcttgag
      541 acgctgacct acccggccga gggcgccgga gcgcgcgtcc gcgaggtcac cgtggcgctc
      601 cgcgcccagg cgcaggagct caacgacgca accgcacgcg cggcggcggc ggcgctcgag
      661 ctgcgccagg cgctcgcggc gcagggccag gagctggtct cggccgcggg cgatctggtg
      721 tcgcgcgcgg ccgcggcgcg cgcagcgctc ggcaacgatg cgcgcgagtt ctccgagctt
      781 gcaggacgga tcgcaaccgg cgtggagaag ctcgaggagg cgcttgcgcg cgaacggcag
      841 atcctcggcg ataccctgac tgaggccgaa acccgcgggc agacgctcaa cggcgtcctg
      901 catgcgcaga cggcgctgat cgccgatgct gcctcccgcg cggcagagag caccacctcg
      961 ctcgagcagg cgctcgcgcg caataccgac accctcgccg ccgcggcgac gcgcgcggac
     1021 gagcaggggg ctgcgatcga tgagcgcctg gccgcggccc gcaccgcgct cgaccagacc
     1081 ggcctgcgcg ccgtcgagcg cgcgggcgag atcgaggccc atctgcgcga gcgcgcggag
     1141 gagctcgccc ggacggccga tggcgccgcc tcgaccgccg tcgtgatcgg cgaggcgctc
     1201 gcgacccata ccaagcagat cgccgccgcg ggtgctaagg cgcatcgcga ggccgagggc
     1261 ctgcgcgagg gtctcgcggg gcagacgcgc gcgctcgagg agttgacgtc cgcccttgcc
     1321 gagcgggcca agaccatcga ggaggccttg agccgccagg ccgagcggct cgaacgtgcc
     1381 tcgcgcccag tcctgcgcct ggcggaggcc gagcgcaagg tgcggcggga gggcttcctc
     1441 gaggcctcca aggcggtgcg cgaggccttg ggctcgatcg cggtcgatct caaccgcgcg
     1501 ctccagcgcg agctgccccg agacgcgatg cagcgctatc aggccggcga tcgcggcatc
     1561 ttcgtgcgca acatcctcgg ctggcgcgac ggcgcgctcg cgcgcgcgat caaggacaag
     1621 tacggctcgg acaatgagtt ccgccgcgcc gtcgacagct acatcgtcaa gttccaggag
     1681 ctgctctccg gggccgacgc cgcggacggc gacgcgctgc tcgctgccac cttccttacc
     1741 tcggacgtgg gcaagctcta catggtcctt gcccgagcga tcgggcggct caaatagcgc
     1801 ggatttcggc atttcccggg ctctcgccgc gaacggcggt ggtcttgcgc tgcgcgccga
     1861 tctaagttct tcgccatgac gcctgccgag ccgaccacgc tgccgcgcgg gaccgcccgg
     1921 cggggccccg cgaccgtccg cgcgctcctc ccctatctct ggccaaaagg ggcgccggag
     1981 ctaaggacgc gggtcgtcat cgccctcctc tttctcgtcg gcgccaagct atgcaacgtc
     2041 tacgtgccga ttctctacaa gcgcgccgtc gaccagctga gcgcgccgcg cccgggggcg
     2101 gccgcgctcg cgcttggcgt gccgctcacc ctcatcctcg gctacggtgc tgcgcgcgtc
     2161 ctgacgctcg ccttcgctga gctgcgtgac gccgtgttcg ccaaggtggc ccagcgcgcg
     2221 atccggcgcg cagcgctcgc taccttcgag cacctccacg ccctctcgct tcgcttccac
     2281 ctcgagcggc agacgggcgg cctcagtcgc gccatcgagc ggggaactgc gggcatcgc
//
LOCUS       DATLKL010000465         1832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_163407, whole genome shotgun sequence.
ACCESSION   DATLKL010000465 DATLKL010000000
VERSION     DATLKL010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163407"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1832
                     /locus_tag="VM713_06210"
     CDS             <1..>1832
                     /locus_tag="VM713_06210"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HVH83891.1"
                     /translation="VNMDEGMLESEAAMTRFLHLVASEPDIARVPVMIDSSKWSVIEA
                     GDYPAALEVARQQVTSGAQVIDVNMDEGMLESEAAMTRFLHLVASEPDIARVPVMIDS
                     SKWSVIEAGLKCVQGKPIVNSISLKEGEEVFIAHARKVRRYGAAAVVMAFDEQGQADS
                     LERRVAVCARAYRILTERVGFPAEDIIFDPNIFAIATGIEEHNAYGVAFIEATRRIKA
                     ELPHALVSGGVSNVSFSFRGNDPVREAMHSVFLYHAIAAGMDMGIVNAGQLGVYEQLP
                     PDLRAACEDVVLNRRADATERLLEIAARFKGEGGVRRSEDLEWRKLPVGKRLEHALVK
                     GIDDYVIADTEEARLAADRPLAVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKR
                     AVAHLVPFIEKSKDAGAQRSNGRIVMATVKGDVHDIGKNIVGVVLQCNNYEVIDLGVM
                     VPCEKILETARREQADFIGLSGLITPSLDEMVHVAREMQRQDSALPLLIGGATTSPAH
                     TSVKIAPQYTGAVVYVKDASRSVGVCQTLSTPTARVAYIDEVRAEHERRREQHANKKV
                     KVPELTIAAARANRRRIDWSSYVAPQPRLPGVHRFADYPLTELLG"
BASE COUNT          315 a          611 c          628 g          278 t
ORIGIN      
        1 gtcaacatgg acgaggggat gctcgagtcg gaagcggcga tgacgcgctt cctgcacctg
       61 gtggcgtccg agcccgacat cgcccgggtg ccggtgatga tcgactcctc caaatggtcg
      121 gtgatcgagg cgggcgacta cccggcagcc ctcgaggtgg cacggcagca ggtcacgagc
      181 ggcgcacagg tcatcgacgt caacatggac gagggcatgc tcgagtcgga ggcggcgatg
      241 acgcgcttcc tgcacctggt ggcgtccgag cccgacatcg cgcgcgtgcc ggtgatgatc
      301 gactcgtcca agtggtcggt gattgaggcc gggctgaaat gcgtccaggg taagccgatc
      361 gtcaactcga tcagcctgaa agagggcgag gaggtcttca tcgcccacgc gcgcaaggtg
      421 cgccgctacg gcgcggccgc ggtcgtcatg gccttcgacg agcagggcca ggccgactcg
      481 ctcgagcggc gggtggcggt ctgcgcgcgc gcctaccgca ttctcaccga gcgcgtcggc
      541 ttcccggccg aggacatcat cttcgatccg aacatcttcg cgatcgcgac cggtatcgag
      601 gagcacaacg cctatggcgt cgcgttcatc gaagctaccc ggcgcatcaa ggccgaacta
      661 ccgcacgcac tggtgtcggg cggcgtgagc aacgtgtcgt tttccttccg cggcaacgac
      721 ccggtgcgcg aggcgatgca ctcggtattt ctctatcacg ccatcgccgc cggcatggac
      781 atggggatcg tgaacgccgg gcagctgggc gtctacgagc agttgccgcc cgatctgcgc
      841 gcagcctgcg aggacgtggt gctcaaccgc cgcgccgatg ccaccgagcg gctgctggag
      901 atcgcggcgc gcttcaaggg cgagggcggc gtgcgccgct ccgaggacct cgagtggcgc
      961 aagctcccgg tcggcaagcg gctcgagcac gccctcgtca agggcatcga cgattacgtc
     1021 atcgcggaca ccgaggaggc gcgcctcgcc gccgatcgcc cgctcgccgt catcgagggc
     1081 ccgctcatgg acggcatgaa cgtggtcggc gacctgttcg gcagcgggaa gatgttcctg
     1141 ccgcaggtgg tcaagagcgc gcgcgtgatg aagcgcgcgg tcgcgcacct cgtgccgttc
     1201 atagagaaga gcaaggacgc gggcgcacag cgctccaacg gccgcatcgt catggcgacc
     1261 gtcaagggcg acgtgcacga catcggcaag aacatcgtcg gcgtggtgct gcaatgtaac
     1321 aactacgagg tcatcgacct cggcgtcatg gtgccgtgcg agaagatcct cgagaccgcg
     1381 cggcgcgagc aggctgactt catcggcctg tcgggtctca tcacgccctc gctggatgag
     1441 atggtgcacg tcgcgcgcga gatgcagcgc caggactccg cgctgccgct gctgatcggc
     1501 ggcgccacta cttccccggc gcacacctcg gtgaagatcg cgccgcaata cacgggcgcc
     1561 gtggtgtacg tgaaggacgc ctcgcgctcg gtgggcgtgt gccagacgct gagcactccc
     1621 accgcgcggg tcgcctatat cgacgaggtg cgcgccgagc acgagcgccg gcgggaacag
     1681 cacgccaaca agaaggtcaa ggtcccggag ctgacgatcg ccgcggcgcg cgccaaccgc
     1741 cgccgcatcg actggagctc ttacgtggct ccccaaccac gcttacccgg agtccaccgc
     1801 ttcgccgact acccgctcac cgagctcctc gg
//
LOCUS       DATLKL010000466         2067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_152339, whole genome shotgun sequence.
ACCESSION   DATLKL010000466 DATLKL010000000
VERSION     DATLKL010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152339"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1192
                     /gene="fliM"
                     /locus_tag="VM713_06215"
     CDS             170..1192
                     /gene="fliM"
                     /locus_tag="VM713_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HVH83892.1"
                     /translation="MAAAGEKVLDQAEIDALIHGVDSGAVNTDPGPAPGEAVSYDFSE
                     QKRIVRGRMPTLEMITERFARLLRTSIYNMLRRSPEIAVHPLQIKKFSEYTHTLHLPT
                     SLNMVKIHPLRGMALMVLDPKLVFAVVDNFFGGAGRHAKIEGREFTVTEQRIIHMLLR
                     SAFSDLREAWAHVANIEVEYLQSEVNPHFANIVSPSEIVVVTSCHIELNGGGGDLHVT
                     MPYSMIEPLRDVLDAGIASDRMEKDTRWSNLLRSEIEDAEVQLKTLLGRATLTLEDLL
                     NLKPGDILPCDFAGKVTLLAEDVPLFRGSFGLSRGQQAVKVETRMRRMGAPPPDPLNF
                     KPESRK"
     gene            1189..1524
                     /gene="fliN"
                     /locus_tag="VM713_06220"
     CDS             1189..1524
                     /gene="fliN"
                     /locus_tag="VM713_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HVH83893.1"
                     /translation="MNANADAVQAAEFEQMQDEGQPASGDVNLDVILDVPVTLSMEVG
                     RTRVPIRNLLQLNQGSVVELDRAAGEPLDVFVNGTLIAHGEVVVVNERFGIRLTDVVS
                     PAERLRKLK"
     gene            1529..1900
                     /gene="fliO"
                     /locus_tag="VM713_06225"
     CDS             1529..1900
                     /gene="fliO"
                     /locus_tag="VM713_06225"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HVH83894.1"
                     /translation="MFAAPQTAPAAHAGALGGLGQVTLALLLVLGAVFALAWLLKRAR
                     TFSSRAGSALDVLAQVPLGQKERAVLLKVGATQILLGVAPGRVNTLYVLPEPLDLTRA
                     AGTGGGEANSPFRNLLRSLSK"
BASE COUNT          335 a          732 c          673 g          327 t
ORIGIN      
        1 cgatctaacc agaagtacgc ccagctctcc agccgcgagg gcaaggaggc gctgcgcgct
       61 ggcgccctcg aagccgtgcg taaagtgctc agctcagccg gcgggcatcc cgagcggctc
      121 gaggcggtgt acttcaccag cttcgtgatg cagtagggag ccggaatcca tggccgcagc
      181 gggtgaaaag gtactggatc aggcggaaat cgacgccctg attcacggcg tcgactccgg
      241 cgccgtcaat actgaccccg gtcccgcccc cggcgaggct gtcagctacg acttctccga
      301 gcagaagcgc atcgtgcgcg ggcgcatgcc gacgctcgag atgatcaccg agcgcttcgc
      361 gcgcctcctg cgcaccagca tctacaacat gctgcgccgc tcgcccgaga tcgcggtgca
      421 tccgctgcag atcaagaaat tcagcgagta cacccatacg ctccatctgc ccaccagcct
      481 caacatggtg aagattcatc cgctgcgcgg catggcgctg atggtgctgg atccgaagct
      541 ggtgttcgcg gtggtcgaca acttcttcgg cggcgcgggc cgccacgcca agatcgaggg
      601 ccgcgagttc accgtcaccg agcagcgcat catccacatg ctgctgcgca gcgccttctc
      661 ggacctgcgc gaggcctggg cgcacgtcgc caacatcgag gtggagtacc tgcagtcgga
      721 ggtcaatccg cacttcgcca acatcgtctc gccgtccgag atcgtcgtgg tgacctcctg
      781 ccacatcgag ctcaacggcg gcggcggcga cctgcacgtg accatgccgt actcgatgat
      841 cgagccgctg cgcgacgtgc tggatgccgg catcgccagc gaccgcatgg agaaggacac
      901 gcgctggagc aacctgctgc gcagcgagat cgaggacgcg gaggtgcagc tgaagacgct
      961 gctgggccgc gccaccctga cgctcgagga tctcctcaat ctcaaacccg gcgacatcct
     1021 gccgtgcgac ttcgccggca aggtgacgct gctcgccgag gacgtgccgc tgttccgcgg
     1081 cagcttcggc ctgtcccgcg gccagcaggc cgtgaaggtc gagacgcgca tgcgccgcat
     1141 gggcgccccg ccccccgatc ccctgaactt caaacccgag agtcgtaaat gaacgccaac
     1201 gccgatgcgg tccaggccgc cgagttcgag cagatgcagg acgagggcca gccggcctca
     1261 ggcgacgtca atctcgacgt catcctggat gtccccgtca cgctgtcgat ggaggtcgga
     1321 cgcacgcgcg tgccgatccg taacctcctg cagctcaacc agggctcggt cgtcgagctc
     1381 gatcgcgcgg ccggcgagcc cctcgatgtc ttcgtgaacg gcaccctcat cgcccacggc
     1441 gaggtggtgg tcgtcaacga gcgcttcggc atccggctca ccgacgtcgt gagtcccgcc
     1501 gagcggctgc gcaagctgaa gtgagccgat gttcgccgcc ccgcaaaccg ctccggctgc
     1561 ccacgccggg gccttaggtg gcctcggcca ggtgacgctg gcactgctgc tggtgctcgg
     1621 cgcggtattc gcgctcgcct ggttgctgaa gcgcgcgcgg accttcagct cgcgggccgg
     1681 cagcgcgctc gacgtgctcg cccaggtgcc gctcggccag aaggagcgcg cagtgctcct
     1741 gaaggtcggg gcgacgcaga ttctcctcgg cgtcgctccc gggcgcgtca acactctcta
     1801 cgtgctgccg gagcctctcg atctgacccg cgccgcgggc acggggggcg gcgaggccaa
     1861 ctccccgttc cgaaacctcc tgcggagtct cagcaaatga gccgcctgcg cctgcccggc
     1921 ctgctcgctc tgctgacgct gctgcccgtc gtagccgcgg ctgcagctcc cgccggtatc
     1981 ccggccgtga ccgtctcggg cgcgggcggc ggacagacct acacgctgtc gctgcaggtg
     2041 ctggcgctga tgacggcgct gacgctg
//
LOCUS       DATLKL010000467         2882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_50572, whole genome shotgun sequence.
ACCESSION   DATLKL010000467 DATLKL010000000
VERSION     DATLKL010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50572"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VM713_06230"
     CDS             complement(<1..306)
                     /locus_tag="VM713_06230"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HVH83895.1"
                     /translation="MASASDRRDLVQGAAIFAGDTVTTGKDARLELTMMDGARITLGD
                     DSELRIDDYVYSPRGKTGRAALMLAHGVFRAVTGALATLAGGPFRVATPVASIGIRGT
                     "
     gene            complement(491..1543)
                     /locus_tag="VM713_06235"
     CDS             complement(491..1543)
                     /locus_tag="VM713_06235"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HVH83896.1"
                     /translation="MKGGAGNFFEDFRVGQELVHATPRTVTSGDTALYLALTGTRFAF
                     HCSDLFARALGLPRAPVDDLLAFHIVFGRTVPDLSLNAIANLGYADCRFLAPVYPGDT
                     LAARSRVIGLRENANRKSGVVYVRSSAANDRGEAVLDYVRWVMVRKRAEGAAPPPTTV
                     PELPPLVPAAALAVPAALTLKSYDFALAGAREIWEDYAPGERIDHVDGVTIEEAEHMM
                     ATRLYQNPARVHFNRRAQEADRFGRRLVYGGHIMSLARALSFNGLANGFKIAAINGGR
                     HVNPTFAGDTIYAWTEVLARWEVPGRRELGAMRLRTIATKDRASAGFPGKDAQGNYDP
                     SVVLELDYTVLIPRRR"
     gene            complement(1559..2449)
                     /locus_tag="VM713_06240"
     CDS             complement(1559..2449)
                     /locus_tag="VM713_06240"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HVH83897.1"
                     /translation="MSQTTRPRRSVLYMPGSNARALEKARTLAADGLILDLEDAVAPD
                     AKALAREQIAAALKTGGYGGRELIVRINGLDTPWGADDLAMVAASRAPAILIPKVERA
                     NMVAEVEARLEAAHAPRELAIWCMMETPLGMLHVEEIATSSPRVGCLVMGTSDLAKDL
                     HAAHTRERLPLLTSLGLCLLAARAFALAILDGVHLDLADDEGFAYACRQGKELGFDGK
                     TLIHPKTIAKANEVFAPSAEEVAWSRRIIGAHASASKEGKGVLLVDGKLIENLHVENA
                     RRLVALADAISTLESAQATQ"
     gene            2655..>2882
                     /locus_tag="VM713_06245"
     CDS             2655..>2882
                     /locus_tag="VM713_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83898.1"
                     /translation="MNVLSIQSFVAFGHVGNRAAILPLERLGHEVWPVNTVSLAHHPG
                     HGAWRGRIATPGEIGTLIDGLEARGALGRCDA"
BASE COUNT          442 a          964 c         1027 g          449 t
ORIGIN      
        1 ggtgccgcgg atgccgatgc tggcgacagg ggttgcgacc cggaaaggcc cgcccgcgag
       61 cgtggccagc gccccggtga ccgcgcgaaa gacgccgtga gccagcatga gtgcggcacg
      121 gcccgtcttg cccctgggcg aatagacata gtcgtcgatc cggagctcgc tgtcgtcgcc
      181 gagggtgatt ctggcaccgt ccatcatggt gagctcgagg cgcgcgtctt tgccggtggt
      241 gaccgtgtcg cctgcgaaga ttgccgcccc ttggacgagg tcgcggcgat cgcttgcgct
      301 cgccacgctc gcctcgcctc tgatgcgcgt gacatggccg gccggatccg cggcgcgggc
      361 caaactcgcc aaggcaagga tcacgatgag ggcgcctagg ccgagccatc gccggcaacg
      421 gcgcgccgac agggaagggt cgattgtcat gcgccattct tgcgcgcgcc gaccgcgccg
      481 acaaggccgg ctagcgccga cgcgggatca gtacggtata gtcgagctcg agcacgaccg
      541 acgggtcgta gttgccctgg gcatccttgc cggggaagcc agccgaggcg cggtccttgg
      601 tcgcgatggt gcgcagccgc atggcaccca gctcgcgccg gcccgggacc tcccagcggg
      661 ccagcacctc ggtccaagcg tagatggtgt ccccggcgaa ggtcgggttc acatgccggc
      721 cgccattgat cgcggcgatc ttgaacccgt tggcgaggcc gttgaagctg agggcgcgcg
      781 ccaggctcat gatgtggccg ccatagacga ggcggcggcc gaagcgatcc gcctcctggg
      841 cgcgccggtt gaagtgcacg cgcgcaggat tctggtagag ccgcgtcgcc atcatgtgct
      901 cggcctcctc gatggtgacg ccgtccacat ggtcgatgcg ctcgcccggt gcgtagtctt
      961 cccagatctc ccgcgcgccg gccagtgcga agtcatagct tttgagcgtg agggctgcag
     1021 gcacggccag ggccgccgcc ggcacaaggg gcggaagctc gggcaccgtc gtcgggggtg
     1081 gtgccgcgcc ctccgcgcgc ttgcgcacca tgacccagcg cacatagtcg agcaccgcct
     1141 cgccgcgatc gttggcggcg ctcgagcgga catagacgac gccgctcttg cggttggcgt
     1201 tttccctcag cccgatcacg cgcgagcgcg ccgcgagcgt gtcgcccggg tacaccggtg
     1261 cgaggaagcg gcaatcggca tagccaagat tggcgattgc gttgagggaa aggtcgggca
     1321 ctgtcctgcc gaagacgatg tggaaggcga ggagatcatc aacgggcgcg cgcggcagcc
     1381 cgagcgcgcg ggcgaagagg tcggagcagt ggaaggcgaa gcgcgtgcca gtgagcgcca
     1441 ggtagagtgc ggtgtcgccg ctcgtgacgg ttcgcggggt ggcgtgcacg agctcctggc
     1501 cgaccctgaa gtcctcgaag aagttgccgg cgcccccctt catcgcgccg atctccgctt
     1561 actgggtcgc ctgggcggat tcgagcgtcg agatcgcatc ggccagcgcc accagccgtc
     1621 gcgcgttctc gacgtgaagg ttctcgatca acttgccgtc gaccagcaac acgcccttgc
     1681 cttccttgct cgccgaggcg tgggcgccga tgatccggcg cgaccaggcg acctcctcgg
     1741 ccgacggcgc gaatacttca ttcgccttgg cgatggtctt cgggtggatg agagtcttgc
     1801 cgtcgaagcc gagctccttg ccctggcggc aggcataggc gaagccctcg tcatcggcga
     1861 gatcgaggtg gacgccgtcc aggatggcga gagcgaacgc gcgcgccgcg aggaggcaga
     1921 gcccgaggct cgtcaagagg ggcaggcgct cgcgcgtgtg cgccgcgtgc aggtccttgg
     1981 cgaggtcgga ggtgcccatg acgaggcagc caacgcgcgg agaggacgtc gcgatctcct
     2041 cgacatggag catgccgagc ggcgtttcca tcatgcacca gatcgcgagt tccctgggcg
     2101 cgtgcgcggc ctccaggcgc gcctccacct ccgctaccat gttagcgcgc tcgaccttgg
     2161 ggatgaggat agcgggcgcg cgcgaggccg cgaccatggc caggtcgtca gccccccagg
     2221 gcgtatcgag cccgttgatg cggacgatga gctcgcgccc gccatagccg cccgtcttca
     2281 gcgccgcagc gatctgctcg cgcgcgagtg ccttggcgtc aggcgccacc gcgtcctcga
     2341 ggtcgaggat gaggccgtcg gccgcaagcg tccgcgcctt ctcgagcgca cgggcgttcg
     2401 agccgggcat gtaaagaacg ctgcggcggg gcctcgtggt ctggctcatg gtgcgccctg
     2461 ttattgcctg tggcccggct gaacgagcgc gcgaacctat caaggaaacg agccgcgaag
     2521 caaggagcgg ctcctaaggg cccaggacgc aatagccttg ccattctgcc atgacgggca
     2581 gccgcgcccg atggccctag gctggccgcc atccctgtgg caggctgacg ccctcgccaa
     2641 cgcatcgcaa accgatgaac gtcctctcga tccagtcctt tgtcgccttc ggccatgtcg
     2701 gaaaccgcgc ggcgatcctg ccgcttgagc ggctcggcca cgaggtctgg ccggtcaata
     2761 cggtgtcgct tgcccatcac cccggccatg gcgcatggcg cgggcgcatc gccacgcccg
     2821 gggagatcgg gactctgatc gacgggctcg aggctcgcgg cgcgctcggc cgctgcgacg
     2881 cg
//
LOCUS       DATLKL010000468         3576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_84481, whole genome shotgun sequence.
ACCESSION   DATLKL010000468 DATLKL010000000
VERSION     DATLKL010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84481"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /gene="gcvPB"
                     /locus_tag="VM713_06250"
     CDS             complement(<1..1285)
                     /gene="gcvPB"
                     /locus_tag="VM713_06250"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HVH83899.1"
                     /translation="MSTREKVIFEYSHPGRGASDQWPHDPGTAALADLPAQLRRAKAP
                     ELPEVGELEAVRHFTRLSQLNFSIDTHFYPLGSCTMKYNPKACNQYAMLPQFLARHPL
                     APESHGQGFLACMYELQEMLKEVTGMQGVALSPMAGAHGEFAGVAMIRAYHRARGDLK
                     RNEIIVPEAAHGTNPATATMCGCVVREIPVNPSGDVDPAALKAVVGPNTAGIMLTNPS
                     TLGVFERGIEEVARVVHEAGGLLYYDGANLNAILGKVRPGDMGFDVMHMNLHKTFSTP
                     HGGGGPGAGAVGVAARLAPFLPIPIVGKEGERYRWLAESDLPQSIGRLSGFMGNAGVL
                     LRAYIYMRLLGRDGMRAVGEYATLNANYLLKRLEAAGFEAAYPERRASHEFIVTLKRQ
                     ARELNVTAMDFAKRLLDLGFHAPTTYFPLIVPECLL"
     gene            complement(1300..2673)
                     /gene="gcvPA"
                     /locus_tag="VM713_06255"
     CDS             complement(1300..2673)
                     /gene="gcvPA"
                     /locus_tag="VM713_06255"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HVH83900.1"
                     /translation="MAFIPHTEADVAAMLATIGVRGIEDLFAEIPPSLRASALAGVPA
                     QLNEMEIGRLMSERAAQDGTPLNFIGAGAYEHHIPAAVWAITTRGEYYSAYTPYQAEA
                     SQGTLQTIYEFQTMIARLTAMEVANASLYDGASATAEAALMAVRANRKSKSARILVPA
                     TLHPHYRRVAATMASNQGLRFEELPYCTVEGVTLPASLGRYAGEDVTALLIQQPNFFG
                     RLEDVDALTDWAHERGILVIAVVNPTSLALLKPPGGWGSRGADIAVGEGQPLGVPLSS
                     GGPYFGFMTTRMEYVRQMPGRIVGRTLDVAGREGFALTLQAREQHIRRAKATSNICTN
                     QGLLVTAATIYLSLMGPQGLARTAAAAHARSRELISALTRTPGVRLAFSGPCFHEAVL
                     LLDRPVAPVLKALAARGILGGLDLAPYYPELGAALLVCATETKTAADLERYRAALGEA
                     MQAARAA"
     gene            complement(2673..3071)
                     /gene="gcvH"
                     /locus_tag="VM713_06260"
     CDS             complement(2673..3071)
                     /gene="gcvH"
                     /locus_tag="VM713_06260"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HVH83901.1"
                     /translation="MSNVPADLKYTKSHEWVRSLADGTVEIGITDHAQHALGDLVFVE
                     VPEAGRTVKEDEPCAVVESVKAASDVYAPLAGEITAGNGKLADAPETLNSDPYGEGWL
                     MRMRPAAGALTAAQLLSAADYQKVLAAEGG"
     gene            complement(3082..>3576)
                     /locus_tag="VM713_06265"
     CDS             complement(3082..>3576)
                     /locus_tag="VM713_06265"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HVH83902.1"
                     /translation="MPAADAVPAWRRLNSLGVASCGLGARDTLRLEAGMNLYGHDMDE
                     STDPFESGLAWTVALEPPGRAFVGREALEAIRARGSQAKLVGLVLEDRGVLRDHQKVL
                     VPGVGEGEITSGTFSPTLERSIALARVPAATAARVQVDIRGKLLNAKVVKPPFVRFGK
                     SLIT"
BASE COUNT          538 a         1193 c         1280 g          565 t
ORIGIN      
        1 tgagcaggca ctccggcacg atcagcggga agtaggtggt cggcgcgtgg aagccgagat
       61 cgagcagccg cttggcgaag tccatggcgg tcacgttgag ctcgcgcgcc tggcgcttca
      121 gcgtcacgat gaactcgtga ctggcgcggc gttcggggta agcggcctcg aagcccgcag
      181 cctccaggcg cttcagcagg tagttggcgt tcaacgtagc gtactcgccc acggcgcgca
      241 tgccgtcgcg tcccagcagg cgcatgtaga tgtaggcgcg cagcagcacg ccggcgttgc
      301 ccatgaaccc cgacagccgc ccgatcgact gcggcaggtc gctctcggcg agccagcggt
      361 agcgctcgcc ctctttgccg acgatcggga tcggcagaaa cggcgcgagg cgcgccgcga
      421 cgcccaccgc gcccgcgccc ggcccgccgc cgccatgggg cgtcgagaag gtcttgtgca
      481 ggttcatgtg catgacgtcg aagcccatgt cgccgggccg caccttgccg aggatcgcgt
      541 tgagattggc gccgtcgtaa tagagcagtc cgccggcctc gtgcaccacg cgcgccacct
      601 cctcgatgcc gcgctcgaac accccgaggg tcgaggggtt ggtgagcatg atgcccgcgg
      661 tattggggcc gactacggcc ttgagcgccg cgggatccac atcgccggac gggttgaccg
      721 ggatctcacg caccacgcat ccgcacatgg tcgcggtggc gggattggta ccgtgcgcgg
      781 cctcggggac gatgatctcg ttgcgcttca gatcgccgcg cgcacggtga taggcgcgga
      841 tcatggcgac gccggcaaat tccccgtgtg caccggccat gggcgagagc gccacgccct
      901 gcatgccggt gacctccttc agcatctcct gcagctcgta catgcaggcg aggaacccct
      961 ggccgtgcga ttccggcgcg agcggatggc gcgccaggaa ctgcggcagc atggcgtact
     1021 ggttacacgc cttggggttg tatttcatgg tgcacgagcc gagcggatag aagtgagtgt
     1081 cgatggagaa gttgagctgc gacaggcggg taaagtgccg caccgcctcg agctcgccga
     1141 cctcggggag ctcgggtgcc ttggcgcgcc gcagctgcgc cggcagatcg gcgagcgccg
     1201 ctgtgccggg atcgtgcggc cactggtctg acgcgccgcg tccgggatgc gagtactcga
     1261 agatcacttt ctcacgagtg gacatcgggg taattcctct caggcggcgc gggccgcctg
     1321 catcgcctca ccgagcgcgg cgcgataacg ctcgaggtca gcggcagtct tggtctcggt
     1381 ggcgcacacc aggagcgctg ccccgagctc cgggtagtac ggggcgaggt cgagcccgcc
     1441 gagaatgccg cgggcggcga gtgccttgag caccggcgca accgggcggt cgagcagcag
     1501 caccgcttca tggaagcacg gtccgctgaa ggcgagacgc acgccgggcg tgcgcgtgag
     1561 cgcactgatg agctcacggc tgcgggcgtg ggcggccgcc gccgtgcgtg cgagtccctg
     1621 cggccccatg agcgagaggt agatcgtcgc cgccgtcacg agcagtccct ggttggtgca
     1681 gatgttggag gtcgccttgg cgcggcggat gtgctgctca cgcgcctgca gcgtcagggc
     1741 gaatccctca cgcccggcga catcgagggt acggccgacg atgcgcccgg gcatctgccg
     1801 cacgtactcc atgcgcgtcg tcatgaagcc gaagtacggg ccgccggacg acagcggcac
     1861 accgagcggc tggccttcgc cgacggcgat gtcggcgccg cggctacccc agccgccggg
     1921 gggtttgagc agggccagcg aggtagggtt gacgaccgcg atcacgagaa tgccgcgctc
     1981 gtgcgcccag tcggtgaggg catccacgtc ctcgagccgg ccgaagaagt tcggctgctg
     2041 gatgaggagc gcggtgacat cctcgcccgc gtacctgccg agcgaggcag gcagggtcac
     2101 tccttcgacg gtgcaatagg gcagctcctc gaatctgagt ccctgattgc tcgccatcgt
     2161 ggccgccacc cgccggtagt gcggatggag cgtcgcgggc acgaggatgc gcgcggactt
     2221 cgacttgcgg ttggcgcgca ccgccatgag cgccgcctcc gcggttgccg aggccccgtc
     2281 gtacagcgag gcgttggcga cctccattgc ggtgaggcgc gcgatcatgg tctggaactc
     2341 gtagatcgtc tgcagcgtgc cctggctggc ttcggcctga tagggcgtgt aggcgctgta
     2401 gtactcgccg cgcgtggtga tggcccacac cgccgcgggg atgtggtgct cgtaggcgcc
     2461 cgcgccgatg aagttgaggg gggtgccgtc ctgggctgcc cgctcgctca tcaggcggcc
     2521 gatctccatc tcgttgagct gcgccggcac tccggcgagc gcgctcgcgc gcaggctcgg
     2581 cgggatctct gcgaacagat cctcgatgcc gcgcacaccg atggtcgcga gcatcgccgc
     2641 gacgtcggcc tcggtatgag ggatgaaggc catcagccgc cctccgctgc cagcactttc
     2701 tgatagtccg ccgccgacag cagctgcgct gccgtgagcg cgccggcggc cgggcgcatg
     2761 cgcatcagcc agccctcgcc gtaggggtcg gagttgagcg tctcgggcgc atcggccagc
     2821 ttgccgttgc cggccgtgat ctcaccggcg agcggcgcgt acacgtcgga tgcggccttc
     2881 accgactcga ccaccgcgca cggctcatcc tctttcaccg tgcgaccggc ttccggcact
     2941 tccacgaaca ccaggtcgcc gagcgcatgc tgcgcgtgat cggtgatgcc gatctcgacc
     3001 gtgccgtcgg cgaggctgcg cacccattcg tgggacttgg tgtacttgag atcggcggga
     3061 acattgctca tgaaggggtc ctcatgtgat cagggatttg ccgaaacgca cgaacggcgg
     3121 cttcaccact ttggcgttga ggagcttgcc gcggatatcc acctgcaccc gtgccgcggt
     3181 tgccgcgggc acgcgggcga gcgcgatcga gcgctcgagc gtcggggaga aggtgccgct
     3241 ggtgatctcg ccctcgccca cgcccggcac caggaccttc tggtgatcgc gcagcacgcc
     3301 gcgatcctcg agcaccaggc cgacgagctt ggcttgcgaa ccacgcgcgc ggatcgcctc
     3361 gagcgcctcg cgcccgacga aggcgcgccc gggtggctcg agcgccacgg tccaggcgag
     3421 tcccgattcg aagggatcgg tgctctcgtc catgtcgtgc ccgtagaggt tcatgccggc
     3481 ttcgaggcgc agcgtgtcgc gcgcgccgag gccgcaggag gcgacgccga gagaattgag
     3541 gcgccgccag gccggcaccg catccgccgc cggcat
//
LOCUS       DATLKL010000469         2122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_219722, whole genome shotgun sequence.
ACCESSION   DATLKL010000469 DATLKL010000000
VERSION     DATLKL010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219722"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VM713_06270"
     CDS             <1..279
                     /locus_tag="VM713_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83903.1"
                     /translation="RRVLVTVEERVERLQAEPNAVVLPAWLITAASVVPGGAYPSYAQ
                     GYYPRDNRFYLAWDEIARDRRTFTAWMERHVLGVSDHAAQLRALGVAA"
     gene            309..1073
                     /locus_tag="VM713_06275"
     CDS             309..1073
                     /locus_tag="VM713_06275"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HVH83904.1"
                     /translation="MTVTAARHLVNGAVCFVGIGLPSAACNLARRTHAPDLVLIYESG
                     TVGTRPRVLPLSIGDGELAETATCVVPLPEIFAHYLQRGRVDVGFLGAAQIDRYGNLN
                     STVIGDYAHPTVRLPGAGGAPEIATYAREVLVIMKQTPRSFVPRLDFRTSCGHLAGSG
                     ARAASGAPGAGPRAVITDFGVLRPHPGTGELELAALYPGITVEAARAATGWPLEVAEH
                     LETIRAPEPADLAALRDLEAATRAAHGRAIRITLRN"
     gene            1075..1989
                     /locus_tag="VM713_06280"
     CDS             1075..1989
                     /locus_tag="VM713_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH83905.1"
                     /translation="MASPPHIRTPLPGPKAQALIARDRPVTTPSYPRDYPFVMARGRG
                     AEAWDVDGNRFLDFMSGIGVASTGHAHPKVVQAIKDAADDFLHISSDYWHERMTRLAE
                     RINELDPMREPVQILICQSGTEAVEGALKLARYVTGRPRFVGFLGGFHGRTMGSLAFT
                     SSKVTQQAGFFPTMPGVTHVPYPNPYRPLFVGADQGRAVLDYIEMLFQSNLPPGEVAA
                     IVVEPIQGEGGYLVPPDSFLPGLRALCDRHGILLIFDEVQCGIGRSGKMFASQHWGVS
                     ADIMTLAKGLASGLPMGLVVARRTHMEK"
BASE COUNT          313 a          784 c          701 g          324 t
ORIGIN      
        1 cggcgcgtgc tcgtcacggt cgaggagcgg gtcgagcggc tgcaggccga acctaacgcc
       61 gtggtgctgc cggcgtggct gatcaccgcc gcgagcgtgg tgccgggcgg cgcctatccc
      121 tcctatgccc agggctacta cccgcgcgac aatcgctttt atctcgcctg ggatgagatc
      181 gcgcgcgacc ggcgaacctt caccgcctgg atggagcgcc acgtgctcgg cgtgtccgat
      241 cacgcagccc agctacgcgc gctcggcgtc gccgcgtgaa cgcccccgcc tacaccgcgg
      301 atgagatcat gacggtgacc gccgcgcggc acctggtgaa cggtgccgtc tgcttcgtgg
      361 gcatcgggct gccgagcgcc gcgtgcaacc tggcgcgccg gacgcatgcc ccggatctgg
      421 tgctcatcta tgaatcaggt acggtcggca cccggccgag ggtgttgccg ctctcgattg
      481 gggacggcga gctcgccgag accgcgacct gcgtcgtacc gctgccggag atcttcgcgc
      541 attacctgca gcgcggccgc gtcgacgtcg gctttctcgg cgcagcgcag atcgatcgtt
      601 acggaaatct gaacagcacc gtcatcggcg actatgcgca cccgaccgtg cgtctgccgg
      661 gcgcgggcgg cgcacccgag atcgcaacct atgcgcgcga ggtgctcgtc atcatgaagc
      721 agacgccgcg cagcttcgtg ccgcggctcg actttcgcac ctcctgcggc catctcgccg
      781 gctcgggtgc gcgcgccgcg agcggcgcac ccggcgcggg cccgcgggcg gtgatcaccg
      841 acttcggcgt gctccggccc catcccggaa ccggggagct cgagctcgcc gccctctatc
      901 ccggcatcac cgtggaggcc gcgcgggccg cgaccggctg gccactcgag gtggccgagc
      961 acctcgagac gattcgcgcc cccgagccgg ccgacctcgc cgcgctgcgc gacctcgaag
     1021 cggctacgcg cgcggcgcac ggtcgcgcca tccgcatcac cctgaggaac tgaaatggca
     1081 tccccaccgc acatccgtac accgctcccc gggccgaagg cacaggcgct catcgcccgc
     1141 gaccggccgg tgacgacacc ctcctacccg cgcgattacc ccttcgtcat ggcgcgcggg
     1201 cgcggcgccg aggcctggga cgtcgacggc aaccgcttcc tcgatttcat gagcggcatc
     1261 ggcgtcgcca gcaccggtca cgcgcacccg aaggtcgtgc aggccatcaa ggacgcggcc
     1321 gatgacttcc tgcacatctc ttccgactac tggcacgagc gcatgacgcg cctcgccgag
     1381 cgcatcaacg agctcgaccc gatgcgcgag cccgtgcaga tactcatctg tcagagcggc
     1441 actgaagcgg tggaaggggc gctcaagctc gcgcgctatg tgaccgggcg cccgcgcttc
     1501 gtcggcttcc tcggcggctt tcacggccgc accatgggct cgctcgcctt cacctcgagc
     1561 aaggtgacgc agcaggcggg attctttccc acgatgccgg gcgtgactca cgtcccctac
     1621 cccaacccct accgcccgct gttcgtcggt gccgaccagg gccgcgcggt cctcgactac
     1681 atcgagatgc tcttccagag caaccttcca ccgggtgaag tggccgcgat cgtggtcgag
     1741 ccgatccagg gggaaggcgg ctacctcgtg ccgcccgaca gcttcctccc cggcctgcgc
     1801 gcgctatgcg accgccatgg gatcctcctc atcttcgatg aggtgcagtg cggcatcggg
     1861 cgcagcggca agatgttcgc gagccagcac tggggcgtgt cagcggacat catgaccctc
     1921 gccaagggcc tcgcttcggg gctgccgatg gggctggtag tcgcgcgccg cacccacatg
     1981 gagaagtgaa gcgcggcgcg catggcaaca ccttcggcgg caatccgctg tgctgcgcgg
     2041 cggcgctcgc cacgctcgac ctcgtcgaac gcgagtacgc ggcgaacgcg gccgcggtcg
     2101 gcgactactt catcgggcgg ct
//
LOCUS       DATLKL010000470         2991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_174646, whole genome shotgun sequence.
ACCESSION   DATLKL010000470 DATLKL010000000
VERSION     DATLKL010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174646"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VM713_06285"
     CDS             complement(<1..553)
                     /locus_tag="VM713_06285"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HVH83906.1"
                     /translation="MNIESPSMPYDFSDLEPAMSRDTVVFHFLRHQRVCYDRMTALAR
                     GAGLGELTLEELIRATEHDPAHHALYRYAAEVWNHNIFRRSMRPRGGGPAEGRVGEEL
                     RQRFGSYERFTHQLKSAASAHFGSGWLWLVWRGAAAEIVVTDNAGTPLVSGETVLLAL
                     DLWEHAYYLDHQNRRAAYVATFLD"
     gene            631..831
                     /locus_tag="VM713_06290"
     CDS             631..831
                     /locus_tag="VM713_06290"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HVH83907.1"
                     /translation="MNRTLVILGVALTLAGLAWPALKRVPLFRLPGDIVVNRPGFTFF
                     LPLTTMLLVSVIVSVIAWLLRR"
     gene            complement(806..1816)
                     /locus_tag="VM713_06295"
     CDS             complement(806..1816)
                     /locus_tag="VM713_06295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HVH83908.1"
                     /translation="MLERALPGAYHVVVDSSRDETKLVTVSFSDVMPPRYHLLDTVSG
                     KLSELGRQDGNLNEALLAPMKPITVPGAGGIAIPGYLTLPPGRAPGERLPAVVLPHGG
                     PYARDHWGYDPLVQVIASRGYAVLQLNFRGSTGYGEQWRDAGHQAWGTIMHEDITAGA
                     HWLIEQGIADPTRMCIVGWSYGGYAALTGVEKEPELYRCAVSIAGVSDMSQMAIDDER
                     FYGGKDSAGDAMGTDPATLRAESPLAHVERIRVPVLLVHGEDDYTVLAEQSKAMDRAL
                     SKQGLRHELVLIRHGEHSLVRPEMRLTLYRKVTEFLRASLSPDQMRAGAPTQVSGAAA
                     TQ"
     gene            complement(1907..2914)
                     /locus_tag="VM713_06300"
     CDS             complement(1907..2914)
                     /locus_tag="VM713_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83909.1"
                     /translation="MAQVLRTLARSLCAAGVVAVPVAWGGPPMRNPAPPILVAPAPPP
                     MRPAMPLRAAVPPVPASGAEVPLEIFAAGPAMADPHLSPDGGKLVYVTSVAGQLRIAV
                     RDLRTGHERTILNAAAGPFSVSHCEFKTNDRLLCHLEGVARGGDPQRLYPASRLLAIN
                     ADGSSLRILFRNAFFARLPPAARSQFQDRIIHWLPNDAYHVLIELGDEGSVFPSVYTL
                     DVYRGDLKLEVPSHPPVMDWTADSAGVVRFGYGFRDDTGVYIARNGPDAPWRTLEEFK
                     RFDRSRFDPLAFGARPNELLVFAPYQRRTAVWQIDLDSAASDLKLVFSHPDVDVGGIL
                     E"
BASE COUNT          458 a         1029 c         1035 g          469 t
ORIGIN      
        1 catcgaggaa cgtggccacg tacgccgcgc ggcggttctg gtgatcgagg taataggcgt
       61 gctcccacag gtcgagcgcg agcagcaccg tctcgcccga caccagcggc gttccagcgt
      121 tgtccgtcac gacgatctcc gcggccgcac cgcgccacac cagccaaagc cagccgctcc
      181 cgaagtgcgc gctggcggcg gatttcagct ggtgcgtgaa acgctcgtac gagccgaagc
      241 gctgacgcag ctcctcgccc acacggcctt cggcggggcc gccgccgcgc gggcgcatcg
      301 agcgcctgaa gatattgtga ttccacacct ccgccgcata gcggtagagc gcatggtgcg
      361 cggggtcgtg ctcggtggcg cggatcagct cctcgagtgt gagctccccg aggccggcgc
      421 cgcgcgcgag cgctgtcatc cggtcgtagc acacgcgctg atgacgcagg aagtggaaca
      481 ccactgtgtc gcgcgacatg gccggctcga ggtcggagaa atcgtacggc atcgacgggg
      541 actcgatgtt catgcagatc ctcccgcaag cacagcgcct tgcttggacc cgcgcgtcgc
      601 gcgccggttc cgcgctaagc ttgccatttg atgaatcgca cgctcgtcat tctcggcgtg
      661 gcgctcacac tcgccggcct cgcgtggcct gcgttgaagc gcgtgccgct gtttcgactt
      721 cccggcgaca tcgtggtcaa tcgcccgggg tttacgttct tcctgccgct gaccaccatg
      781 ctgctcgtga gtgtgatcgt ctcggtcatt gcgtggctgc tgcgccgctg acctgcgtgg
      841 gggcacccgc acgcatctgg tccgggctga ggctcgcgcg taaaaactcg gtgaccttcc
      901 gatacaacgt cagccgcatt tccgggcgca ccaggctgtg ctcgccgtgc ctgatgagca
      961 ccagctcgtg ccgcaggccc tgcttcgata gcgcgcggtc cattgccttg ctctgctcgg
     1021 cgagcaccgt gtagtcatcc tcgccgtgca ccagcagcac cggcacgcgg atgcgctcga
     1081 catgcgcgag cggcgactcc gcgcgcagcg tcgccgggtc ggtgcccatg gcatcgcccg
     1141 cggagtcctt gccgccgtag aagcgctcgt cgtcgatggc catctgcgac atatcggaca
     1201 ccccggcgat gctgaccgcg caacggtaga gctccggctc cttctccacg ccggtgagcg
     1261 ccgcatagcc gccgtagctc cagcccacga tgcacattcg cgtgggatcg gcgatcccct
     1321 gctcgatgag ccagtgggcg ccggcggtga tgtcctcgtg catgatcgtg ccccacgcct
     1381 ggtgcccggc gtcgcgccac tgctcgccgt acccggtcga gccgcggaag ttgagctgca
     1441 gcacggcgta gccccggctc gcgatcacct gcaccagcgg atcgtagccc cagtgatcgc
     1501 gcgcgtacgg cccgccatgc ggcagcacca cggcgggtag ccgctccccc ggcgctctcc
     1561 ccggtggcag cgtcaggtag cccgggatgg cgatgccgcc cgcccccggc acggtgatgg
     1621 gcttcatggg cgcaagcagc gcctcgttga ggttgccgtc ctgccggccg agctcgctga
     1681 gcttgccgct caccgtatcg agcaggtggt agcgcggcgg catcacgtca ctgaaggaga
     1741 ccgtgacgag cttggtctcg tcgcggctcg agtcgaccac cacgtgatac gcgccgggaa
     1801 gcgcgcgctc cagcatgtcc tcgacgctcg cggcgtgcgg gtcgatgaag tgcgtatgcg
     1861 gatggtcggt ctcgtagacg aagccggcga cgtgacggtc gagcggtcac tcgaggatgc
     1921 cgcccacgtc gacgtcgggg tgcgagaaca cgagcttgag gtcgctcgcc gcgctgtcga
     1981 ggtcgatctg ccagaccgcg gtgcgccgct ggtacggggc aaacaccagc agctcattgg
     2041 ggcgcgctcc gaaggcgagc ggatcgaacc gcgagcggtc gaaacgcttg aactcctcga
     2101 gcgtgcgcca gggcgcgtcg gggccgttgc gggcgatgta aacgccggtg tcgtcgcgga
     2161 agccgtagcc gaagcgcacc acgcccgccg agtccgcggt ccaatccatc accggcgggt
     2221 gcgaagggac ctcgagcttg aggtcccccc ggtagacgtc gagcgtgtag accgaaggga
     2281 agacgctgcc ctcgtccccg agctcgatga gcacgtgata ggcatcgttc ggcagccagt
     2341 ggatgatgcg gtcctggaac tgggagcgag ctgccggggg cagccgcgcg aagaaggcgt
     2401 tgcggaagag tattcgcaac gagctgccgt cggcgttgat ggcgagcagc cgcgaggccg
     2461 gatagagtcg ctgggggtcg cccccgcgcg cgacgccctc gaggtggcac agcagccggt
     2521 cgttggtctt gaactcgcag tggctcaccg agaaggggcc cgcggccgca ttgaggatcg
     2581 tgcgctcgtg tccggtacgg aggtcgcgca ccgcgatgcg cagctgcccc gccacgctcg
     2641 tgacatacac gagcttgccg ccgtccggcg agaggtgcgg atcggccatc gcaggtcctg
     2701 cggcaaagat ctccagcggg acctccgcac ccgaggccgg taccggcggc acggcggccc
     2761 ggagcggcat cgcgggccgc atcggcggcg gcgccggagc cacgaggatc ggcggggctg
     2821 gattgcgcat cggcgggccg ccccaggcca ccggcaccgc gacgactcct gcggcgcata
     2881 acgaccgggc gagcgtgcgc aggacctgcg ccatcggaac ctcctcgctc acgaccccgc
     2941 tgctgcgagc gcgcgagtga catcctcgat cagatcaccg aggtcctcaa g
//
LOCUS       DATLKL010000471         4009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_84524, whole genome shotgun sequence.
ACCESSION   DATLKL010000471 DATLKL010000000
VERSION     DATLKL010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84524"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..478
                     /locus_tag="VM713_06305"
     CDS             59..478
                     /locus_tag="VM713_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVH83910.1"
                     /translation="MKYLHTMVRVTDIDASLRFYCDALGLKELSRKDYPQGRYTLVFL
                     AAPGDESAQVELTHNWDPEAYGGGRNFGHLAYQVEDIYATCQRLRDHGVTILRPPRDG
                     RMAFVRSPDHISIELLQKGAALAAAEPWKSMANTGAW"
     gene            510..3152
                     /locus_tag="VM713_06310"
     CDS             510..3152
                     /locus_tag="VM713_06310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVH83911.1"
                     /translation="MKTIPSLIIAAVFATLTFAVWAYLNRPTREPPWPARIQGFAFSP
                     FRANEDPTTFTMPTDAEIDADLALLEGKVNAVRTYSVGATLADVPELADRHGLNVALG
                     AWLDNHLDKNEEQLKTAIDLAGTHQNVVRVVVGNEVLLRGDLTLEQLEKYLDRARAAI
                     GQPVGTAETWHAWLTHPELAQHVDYIGVHLLPYWEGVPVEAAVEYSFAQFKRLQHAFP
                     HKPIVIAEIGWPSRGRTRESAVASEANEALFLRRFLNRAEREQIKYYVMEAFDQPWKA
                     YQEGAVGSYWGVYDVNRQPKFAFTEPIVRVPEWHILAALSVAAALLVLGLVYLTSAAL
                     RNRGRSFLAIVVYAATTHAVWVIYDFTQQYMTVSSVIAGVLLLLGMLGVLLVLLAEAH
                     EWAEAHWVEYRRRLGAPMFTAGEQRPKVSIHVPAYNEPPDMLIETLDALARLDYPDFE
                     VLVIDNNTRDESVWRPVEAHCAKLGARFHFFHVAPLAGFKAGALNYALERTAPDAAIV
                     AVIDSDYVVDPRWLRELTPAFQDPRIAIVQAPQDYRDANQSAFKAMCYAEYRGFFHIG
                     MITRNERNAIIQHGTMTMVRRPQLERCRWAEWCITEDAELGLRIFEAGLDASYVPASY
                     GRGLMPDTFIDFKKQRFRWAYGAMQILKAHARALFLEGGPLSPGQRYHFVAGWLPWVA
                     DGSNLLFNLAALGWSVAMVWAPIEPPLVMYSVLPLSLFTFKLAKLVHLYRVRVGANVR
                     QTVAAAVAGLALTHTIGTAAVKGLVTRSEPFFRTPKQGRTSGLLHALAAAREETLLMS
                     GLMLSAWAVAHLGLNRVQGPEGFAWGPDRVAWIVVLLVQSVPYASSLVVSLMSAFPLP
                     ARLLGTSYRPVAAVRRQAHAGPVA"
     gene            3159..>4009
                     /locus_tag="VM713_06315"
     CDS             3159..>4009
                     /locus_tag="VM713_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVH83912.1"
                     /translation="MRERRLQAIATMIAAVAVFSFMDAVLKFFAMRYPPMEVAALRGA
                     TSLPFTVAPVLIAGRLRDLVPQRWPMHLLRGCLSVVVIGGFVYAVRSLSLANAYSVFL
                     SAPLLVAALSVPLLRERLDWRNWLAILAGLAGVLVMLRPTTSGLASIGAVAALIGATA
                     YALSGIAVRVLTRTDTTVSVVFWTISLMTVLAGAVAAPGWVGIEPGHWKWLLALGVLA
                     AAGQYLLTEAFRSAPPSVVSPFEYTALLWGVGIDRVVWHVLPSARVCLGGGIVIASGL
                     YLIWRER"
BASE COUNT          557 a         1410 c         1360 g          682 t
ORIGIN      
        1 gcgccggccc tcccgtacac cggcggttaa gacgcgcgcg gttaatctgg agaagcccat
       61 gaaatacctc cacaccatgg tgcgcgtcac cgacatcgac gcgagtctgc gcttctactg
      121 cgatgcgctc ggcctcaagg aactgtcccg caaggactac ccgcaggggc gctacacgct
      181 ggtgttcctc gccgctcccg gcgatgagag cgcccaggtc gagcttaccc acaactggga
      241 cccggaggcc tatggcggcg ggcgcaactt cgggcacctt gcctaccagg tcgaggacat
      301 ttacgccacc tgccagcgtc tgcgcgacca cggcgtcacg atcctgcggc cgccgcgcga
      361 cgggcgcatg gcattcgtgc gctcgcccga ccacatctcg atcgagctgc tgcaaaaggg
      421 cgcagcgctc gccgcggctg agccctggaa gtcgatggcc aacaccggcg cctggtaggc
      481 tgagtgcgcg ctagacaccc cctcagccca tgaagacgat cccgagcctc atcatcgcgg
      541 cggtgttcgc cacgctgacc tttgccgtgt gggcgtacct caaccgcccg acgcgcgagc
      601 cgccgtggcc ggcgcgcatc caggggttcg ccttctcgcc cttccgggcc aacgaggatc
      661 ccacgacttt cacgatgccg acggacgccg agatcgacgc cgacctcgcg ctgctcgagg
      721 gcaaggtgaa tgcggtgcgc acctactcgg tcggcgcgac gcttgccgac gtgcccgagc
      781 ttgccgaccg ccacggcctg aacgtcgcgc tcggcgcctg gctcgataac cacctcgaca
      841 agaatgaaga gcagctgaag accgccatcg acctcgccgg cacgcaccag aacgtggtgc
      901 gggtcgtggt gggcaacgag gtgctgctgc gcggtgatct gacgctcgag cagctcgaga
      961 agtatctcga ccgggcccgc gccgccatcg gccagccggt cgggaccgcc gagacctggc
     1021 acgcctggct cacgcacccc gagctcgccc agcacgtcga ctatatcggc gtgcacctgc
     1081 tgccctactg ggagggcgtc ccggtggagg ccgcggtcga atactccttc gcgcagttca
     1141 agcgcctgca gcatgccttc ccgcacaagc cgatcgtgat cgccgagatc ggttggccga
     1201 gccgcgggcg cacgcgcgag tcggcggtgg cctcggaggc caacgaggcg ctgttcctgc
     1261 gccgcttcct caaccgcgcc gagcgcgagc agatcaaata ctacgtgatg gaggccttcg
     1321 atcagccgtg gaaggcgtat caggaggggg cggtcggctc ctactggggc gtgtacgacg
     1381 tcaaccggca gccgaagttt gctttcaccg agcccatcgt gcgcgtgccc gagtggcaca
     1441 tcctggcggc gctctcggtt gcggccgcgc tgctcgtgct cgggctcgtg tatctcacca
     1501 gcgctgcgct gcgcaaccgc ggccgctcgt tcctcgctat cgtggtgtac gccgccacga
     1561 cgcacgccgt gtgggtgatc tacgacttca cccagcagta catgacggtc tcgagcgtga
     1621 tcgcgggcgt gctgttgctg ctcggcatgc tcggggtgct gctggtgctg ctcgccgagg
     1681 cgcacgaatg ggccgaggca cactgggtgg agtaccggcg ccggctcggc gcgccgatgt
     1741 tcaccgccgg cgagcagcga cccaaggtct cgattcacgt gccggcctac aacgagccgc
     1801 ccgacatgct catcgagacg ctcgacgccc tcgcgcggct cgactatccc gacttcgaag
     1861 tgctggtcat cgacaacaac acccgcgacg agagcgtctg gcgtccggtc gaggcgcact
     1921 gcgcaaagct cggagcgcgc ttccacttct tccacgtggc gccgcttgcg ggcttcaagg
     1981 caggcgcgct caactacgcg ctcgagcgca ccgctcccga cgccgccatc gtcgccgtca
     2041 tcgacagcga ctacgtggtg gatccgcgct ggctgcgcga gctgacgccc gccttccagg
     2101 acccgcgcat cgccatcgtg caggcgccgc aggattaccg cgacgcgaat cagagcgcct
     2161 tcaaggccat gtgctatgcc gagtaccgcg gcttcttcca catcggcatg atcacgcgca
     2221 acgagcgcaa cgccatcatc cagcacggca ccatgaccat ggtgcgccgc ccgcagctcg
     2281 agcgctgccg ctgggcggag tggtgcatca ccgaggatgc cgagctcggc ctgcgcatct
     2341 tcgaggcggg gctcgacgcg agctacgtgc cggcgagcta cggccgcggg ctgatgcccg
     2401 acacgttcat cgacttcaag aagcagcgct tccgctgggc ctacggcgcg atgcagatcc
     2461 tcaaggcgca cgcacgcgcg ctgttcctcg agggcggtcc gctctcgccc gggcagcgct
     2521 accacttcgt cgccggctgg ctgccatggg tcgccgacgg ctccaacctg ctgttcaacc
     2581 tggccgccct cggctggtcg gtcgccatgg tgtgggcgcc gatcgagccg ccgctcgtca
     2641 tgtactcggt gctgccgctg tcactgttca ccttcaagct cgccaagctc gtgcacctgt
     2701 atcgcgtgcg ggtgggcgcc aacgtcaggc agacggtggc ggcggccgtc gcgggactcg
     2761 ccctgaccca cacgatcggc acggcggcgg tcaagggcct ggtcacgcgc agcgagccct
     2821 tcttccgcac gcccaagcag ggccgcacct cggggctcct gcacgcgctc gcggcggcgc
     2881 gcgaggagac gctgctgatg agcgggctga tgctgtcggc gtgggcggtg gcgcaccttg
     2941 ggctcaaccg tgtgcagggg ccggagggct tcgcctgggg accggaccgc gtggcgtgga
     3001 tcgtggtgct gctggtgcag tcggtgccgt acgcctcatc gctggtggtc tcgctgatga
     3061 gcgccttccc gctgccggcg cgcctgctcg gcacgagcta tcgcccggtg gccgctgtca
     3121 ggcgccaggc gcacgcgggg cccgtggctt aactctccgt gcgcgagcgc aggctccagg
     3181 ccatcgcgac catgatcgcc gcggtcgcgg tgttctcctt catggacgcg gtcttgaagt
     3241 tctttgcgat gcgctatccg ccgatggagg tggcggcgct gcgcggggct acctcgctcc
     3301 ccttcacggt cgcaccggtg ctgatcgcgg ggcgtctgcg cgatctcgtg ccgcagcgct
     3361 ggccgatgca cctgctgcgc ggctgcctga gcgtcgtggt gatcggcggc ttcgtttacg
     3421 cggtccgctc tctgtcgctc gccaatgctt attcggtttt cctgtcggcg ccgctgctgg
     3481 tggcggcgct gtcggtgccg ctgctgcggg agcggctcga ctggcgcaac tggctggcga
     3541 ttctcgccgg gctggccggc gtcctggtca tgctccgacc cactacctcg gggctggcga
     3601 gcatcggtgc cgtcgcggcg ctcatcggcg ccaccgccta cgccctgagc ggtattgcgg
     3661 tgcgggtcct cacccggacc gacacgacgg tgagcgtcgt cttctggacg atcagcctca
     3721 tgaccgtcct ggccggcgcc gtggcggccc cgggctgggt cgggatcgag ccggggcatt
     3781 ggaaatggct gctggccctg ggggtcctcg ccgcggccgg gcagtacctc ctcaccgagg
     3841 cgttccgctc ggcgccgccc tcggtggtgt ccccgttcga gtacaccgcg ctcctctggg
     3901 gtgtcgggat cgaccgagtg gtgtggcacg tactgccctc ggctcgggtg tgcctgggcg
     3961 ggggtattgt gatcgcgagc gggctctacc tgatctggcg cgagcgcag
//
LOCUS       DATLKL010000472         2420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_152411, whole genome shotgun sequence.
ACCESSION   DATLKL010000472 DATLKL010000000
VERSION     DATLKL010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152411"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VM713_06320"
     CDS             <1..1213
                     /locus_tag="VM713_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HVH83913.1"
                     /translation="DRGSRLVVLELDTPGGLDSSMRDIIRAILASPVPVASYVTPSGA
                     RAASAGTYILYASHVAAMAPATNLGAATPVSIGGEPEAPAPPVLPQGKPADKGGGAGE
                     GSAPQSPPGTAMERKVVNDAVAYIRGLAELRGRNADWAEQAVRGAASLTANAALEQKV
                     IDLVARDLPDLLARCDGREVRIGDHTLKLASRELVAVSVKPDWRTELLAVITNPTVAY
                     GLMLIGIWGLLLEGYNPGAVLPGVVGSICLLVALFAFQILSVNYAGLALLVLGTAMII
                     AEFFFPTYGSLGAGGVVAFIVGSLILFDTDVPGIHIARSLIAAIATVGALVIAGIVYL
                     ATRAQRQPVATGAQAMVGDVAEVVADFSGRGRVRYGGELWNARSTVALHTGDMVRIVK
                     VEGLTLWIEPQ"
     gene            1242..1985
                     /locus_tag="VM713_06325"
     CDS             1242..1985
                     /locus_tag="VM713_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HVH83914.1"
                     /translation="MPFVIALVILFIILFFSTVNVLNEYERLVVFTLGRFAGVRGPGL
                     VLIVPGIQRTRRVDLRVIVLSVPKQDVITRDNVSVKVNAVIYFRVVDPGKAIIQVANA
                     FDATSQVAQTTLRSVVGQHEMDDLLSQRDKLNVDLQRILDQSTESWGIKVSTVEIKDV
                     DLDETMIRAMAKQAEAERTRRAKIINAEGEQQAAQRLVEAATLLATQPSAMQLRYLQT
                     LADIAHERTQLLVFPLPLDLIKPLLGGKG"
     gene            2038..>2420
                     /locus_tag="VM713_06330"
     CDS             2038..>2420
                     /locus_tag="VM713_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83915.1"
                     /translation="MTVGPDSSGLPIAAALPALREALGARGAAVLEAPPGAGKSTVAP
                     LALLDEPWALGQRLIVLEPRRLAARAIAQRLADSLGETVGQTVGYRMRLDTRVSSGTR
                     LEVVTEGVLTRMLQEDPALEGVAGLL"
BASE COUNT          360 a          839 c          855 g          366 t
ORIGIN      
        1 cgatcgcggc agtcgcctgg tcgtgctcga gctggacacc ccgggcgggc tcgacagttc
       61 gatgcgcgac atcatccgcg cgatcctcgc ctcgcccgtg ccggtcgcga gctatgtcac
      121 gccgtcgggg gcgcgcgcgg cgagtgccgg aacctacatc ctctatgcga gccacgtcgc
      181 cgccatggcg ccggccacca atctcggcgc cgcaaccccg gtgtcgatcg gcggcgagcc
      241 ggaggctccg gctccgcccg tactgccgca gggcaagccc gcggacaagg gcggcggcgc
      301 gggcgagggc agcgccccgc agagcccgcc ggggaccgcc atggagcgca aggtggtgaa
      361 cgatgccgtc gcctacatcc gcgggctcgc ggagctgcgc ggccgcaacg ccgactgggc
      421 cgagcaggcg gtgcgcggcg cggcgagtct caccgcgaac gccgctctcg agcagaaggt
      481 gatcgatctc gtcgcgcgcg acctgccgga tctgctcgcc cgctgcgacg gccgcgaagt
      541 acgcatcggc gatcacacgc tcaagctcgc cagccgcgag ctcgtggcgg tgagcgtcaa
      601 gcccgactgg cgcaccgagc tgctcgcggt cattaccaac ccgaccgtcg cctacggcct
      661 catgctcatc gggatctggg gcctgctgct cgaggggtac aatccgggag cggtgctgcc
      721 cggcgtggtc ggctcgatct gcctgctggt ggcgctgttc gcgttccaga tcctgtcggt
      781 gaattacgcg gggctggcgc tgctggtgct cggcaccgcc atgatcatcg cggagttctt
      841 cttcccgacc tacggctcgc tcggcgcggg gggtgtggtt gcgttcatcg tcggctcgct
      901 catcctgttc gatacggacg tgccgggcat acacatcgcg cgctcgctga tcgccgcgat
      961 cgccacggtc ggcgccctgg tgatcgccgg catcgtgtat cttgcgacgc gcgcgcagcg
     1021 ccagccggtg gcgaccggcg cgcaggccat ggtcggcgac gtggcggagg tcgtggccga
     1081 cttcagcggc cggggccggg tgcgctacgg cggcgagctg tggaacgccc gcagcacggt
     1141 cgcgctgcat accggcgaca tggtgcgcat cgtcaaggtg gaaggtctaa cattgtggat
     1201 cgagccgcag tgagcgcggc gcgcaccacc cggagaattg catgccgttc gtgatcgccc
     1261 tagtcatcct cttcatcatc ctgtttttca gcaccgtgaa cgtgctgaac gaatacgagc
     1321 gactggtggt gttcacgctc ggacgcttcg ccggcgtcag ggggccggga ctcgtgctga
     1381 tcgtccccgg catccagcgc acccgccggg tggacctgcg ggtcatcgtg ctgagcgtgc
     1441 ccaagcagga cgtgatcacg cgcgacaacg tctcagtcaa ggtgaacgcg gtcatctact
     1501 tccgcgtggt cgatcccggc aaggccatca tccaggtggc caacgccttc gatgccacca
     1561 gccaggtggc ccagacgacg ctccgctcgg tggtcggcca gcacgagatg gacgatctgc
     1621 tctcgcaacg cgacaagctc aacgtcgacc tgcagcgcat cctcgaccag agcaccgagt
     1681 cctggggcat caaggtgtcc accgtcgaga tcaaggacgt ggatctcgat gagaccatga
     1741 tccgcgccat ggcgaagcag gccgaggccg agcgcacccg ccgcgccaag atcatcaacg
     1801 ccgagggcga gcagcaggcg gcgcaaaggc tggtcgaggc ggcgacgctg ctggcgacgc
     1861 agccgagcgc catgcagctc aggtacctgc agacgctcgc cgacatcgcg cacgagcgca
     1921 cccagctgct ggtcttcccg ctgccgctcg atctcatcaa gccgctgctc ggcggcaagg
     1981 gataagcact cacggcgctt gcggcggcac ccggcgggcg cagcgcgttc acatctggtg
     2041 acggtgggtc cggacagctc ggggctgccg atcgcggcgg cgctgccggc gctccgcgag
     2101 gcgctcggcg cgcgcggcgc cgcggtgctc gaggcgccgc ccggcgcggg caagagcacg
     2161 gtggcgccgc tggcgctgct cgatgagccc tgggcgctcg gccagcggct cattgttctc
     2221 gagccacgcc gcctcgcggc gcgcgcaatc gcgcagcgtc tcgcagactc actcggcgag
     2281 acggtcgggc aaacggtcgg gtaccgcatg cggctcgaca cgcgcgtatc gagcggtacg
     2341 cgcctcgagg tcgtcaccga aggggtgctc acccgcatgc tgcaggagga ccccgcactc
     2401 gagggggtgg cggggctgct
//
LOCUS       DATLKL010000473         1781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_107104, whole genome shotgun sequence.
ACCESSION   DATLKL010000473 DATLKL010000000
VERSION     DATLKL010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107104"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /gene="queG"
                     /locus_tag="VM713_06335"
     CDS             complement(<1..770)
                     /gene="queG"
                     /locus_tag="VM713_06335"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HVH83916.1"
                     /translation="MTGPAAIDLDAAKSLLVAEARALGFNALGVTAIDIPEDERHLLR
                     WLGEGFHGEMGYMRRHARLRGSPQELLPGTVRVVSVRMDYWPGEARAAADVLRDSTLG
                     YVSRYALGRDYHKVLRHSLARLAADLARRIGGFGYRVCVDSAPVLEKAHARNAGVGWI
                     GKHTNLIARDAGSWFFLGEILTDLPLPVDERASAHCGSCRACIPACPTAAIIAPYRLD
                     ARRCVSHLTIEHHSAIPEELRAALGNRIYGCDDCQLVCP"
     gene            803..>1781
                     /locus_tag="VM713_06340"
     CDS             803..>1781
                     /locus_tag="VM713_06340"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HVH83917.1"
                     /translation="MALPIALYSSAQVRALDAHAIEVLGIPAYTLMKRAGEAALRHLR
                     SRYPVAHRIAVVCGGGNNGGDGYVLARFAQAAGLTVTVLAASEPEELRGAARQAFEDF
                     RATGARARPFAAALLAEGEVVVDALLGTGLRGAVRTEYAAAIRAINSSGRPVFALDVP
                     SGLDSDAGVALGEAVRADGTVTFVALKTGLLVGEGPEYTGTVFFDDLELTAPAGEFAP
                     RLTRIVESEIHAALPRRHRAAHKGDFGRVLLVGSGSGMPGATRLAGEACLRVGAGLTT
                     VAVAPENVAAVAAGRPELICLALTDSRVLTEALARADVVAIGPGLGRSPW"
BASE COUNT          237 a          614 c          677 g          253 t
ORIGIN      
        1 gggcacacca gctggcagtc gtcgcagccg tagatgcggt tcccgagcgc cgcgcgcagc
       61 tcctcgggaa tagcgctgtg atgctcgatg gtgagatggg agacgcagcg ccgtgcgtcg
      121 agccgatacg gcgcgatgat cgctgcggtg ggacaggccg gaatgcacgc ccggcagctg
      181 ccgcagtggg cgctggcgcg ctcgtccacc ggcagcggca ggtcggtcag gatctcgccg
      241 aggaagaacc acgagccggc atcgcgcgcg atcaggttgg tgtgcttgcc gatccagccg
      301 acaccggcgt tgcgcgcgtg cgccttctcg agcaccggcg cgctgtcgac gcacacgcgg
      361 tagccaaagc cgccgatgcg ccgggcgaga tccgcggcga gccgcgcgag cgagtgacgc
      421 agcaccttgt ggtagtcgcg gccgagcgcg tagcgcgaga cgtagccgag cgtgctgtcc
      481 cgcagcacgt ccgcggcggc gcgcgcctcg cccggccagt agtccatgcg cacgctcacc
      541 acgcgcaccg tgccgggcag cagctcctgc gggctgccgc gcaggcgggc gtggcgccgc
      601 atgtacccca tctcgccgtg gaagccctcg ccgagccatc tgaggaggtg ccgctcgtcc
      661 tcagggatgt cgatggcggt cactccgagg gcgttgaagc cgagggcgcg cgcctcggcc
      721 acgagcaagc tcttggccgc gtcaaggtcg atggcagcgg gtccggtcac ggcactatca
      781 cgcacgtcag tataatgagc cgatggcgct gccgattgcc ttgtactcca gcgcgcaggt
      841 gcgagccctc gacgcgcacg cgatcgaggt gctggggatc ccggcctaca ccctgatgaa
      901 gcgcgccggc gaggcagcgc tgcgccacct gcgctcgcgc taccccgtgg cgcatcgcat
      961 tgcggtcgtc tgcggcggcg gcaacaacgg gggcgacggc tacgtgttgg cgcgcttcgc
     1021 gcaggcggcg ggactcaccg tcaccgtgct cgcggcgagc gaaccggagg agctgcgcgg
     1081 cgcggcgcgc caggccttcg aggactttcg cgcgaccggc gcgcgggcgc gcccgtttgc
     1141 ggcagcgctc ctggccgagg gcgaggtcgt ggtggacgcg ctgctcggca cgggacttcg
     1201 cggcgcggtt cgtacggagt acgccgcggc catccgcgcc atcaactcga gcgggcgccc
     1261 ggtgtttgcg ctcgacgtac cgtcgggact cgacagcgat gccggcgttg cgctcggcga
     1321 ggcggtgcgc gccgacggca cggtcacgtt tgtcgcgctg aagaccgggc ttctggtcgg
     1381 ggagggtccg gaatacaccg ggaccgtgtt cttcgacgat ctcgagctca ccgccccggc
     1441 cggtgagttt gcgccgcgcc tgacgcgcat cgtcgagagc gagatccacg cggcgctgcc
     1501 gcggcggcac cgggccgccc acaagggcga tttcggccgg gtgctgctcg tcggcagcgg
     1561 cagcggcatg ccgggcgcaa cccgtctcgc gggcgaggcc tgtctgcgcg tcggggccgg
     1621 gctcacgacg gtcgcggtgg cgcccgagaa cgtggcggcg gtcgcggccg gcaggcccga
     1681 gctcatttgt ctggcgctca ccgattcgcg cgtgctcacg gaagcactcg cgcgcgcgga
     1741 cgtggtggcg atcggcccag gcctcggtcg cagtccctgg g
//
LOCUS       DATLKL010000474         1604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_186164, whole genome shotgun sequence.
ACCESSION   DATLKL010000474 DATLKL010000000
VERSION     DATLKL010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186164"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VM713_06345"
     CDS             <1..1103
                     /locus_tag="VM713_06345"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HVH83918.1"
                     /translation="MTVGAGRRCSTANAYLRPAMERTNLTVRTHALATRILFDGRRAV
                     GVRYRRGGALHDVLVRREVIVCAGAVNSPQLLKLSGLGPRAELAAHSIAVVHELPGVG
                     ENLQDHLEFYFQMACREPITLYAEMNPWRKTLIGLRWLLRHDGLGVSNHFETGGFIRS
                     RPGVRYPDIQFHFLPMAVSYDGSSLAREHGFQAHVGPMRSHSRGWVRLKSADPLEKPR
                     VFFNYMSQPQDWEEMRACVRLTREIFAQPAFARYRGREIQPGEPVQSDAEIDAFIRAK
                     VESAFHPSCSCRMGRASDPLAVVDAQARVIGTQALRVVDSSIMPSITTGNLNAPTIML
                     AEKAADYIRGRALLPPSAAPYYVAADWESKQR"
     gene            complement(1100..>1604)
                     /locus_tag="VM713_06350"
     CDS             complement(1100..>1604)
                     /locus_tag="VM713_06350"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HVH83919.1"
                     /translation="TGRPRFIDKMPNNFRHLGLIHLMLPNARIVDARREPMACCFGNF
                     KQLFAKGQEFTYGIEDIARYYRTYLELMRHWERVLPGRVLRVQHEDLVNDLEGNVRRL
                     LEYCGLGFEPACVEFYRTERSIRTASSEQVRQPIYREGLDQWRHYEPSLESLRAALGD
                     ALTRYRD"
BASE COUNT          248 a          552 c          538 g          266 t
ORIGIN      
        1 agatgacggt cggcgcgggc cggcgctgca gcaccgccaa cgcgtatctg cgtccggcga
       61 tggagcggac caatctcacg gtgcgcaccc acgcgctcgc gacgcggatc ctcttcgacg
      121 ggcggcgcgc ggtgggagtg cgctaccgcc gcggcggggc gctgcacgac gtgctggtgc
      181 ggcgcgaagt gatcgtgtgt gcgggcgccg tgaactcccc gcagctgctc aagctctcag
      241 gattgggccc gcgggctgag ctcgccgcgc acagcatcgc cgtcgtgcac gagctccccg
      301 gagtcggcga gaacctgcag gatcacctcg agttctactt tcagatggcc tgccgggagc
      361 cgattacgct ctacgcggag atgaatccct ggcgcaagac gctgatcggc ctgcgctggc
      421 tgctgcggca cgacggcctc ggtgtttcca atcactttga gaccggcggc ttcatccgca
      481 gccgccccgg ggtgcgctac ccggatattc agtttcattt cctgcccatg gcggtgagct
      541 acgacggctc ctcgctcgcc cgcgagcacg gcttccaggc gcacgtcgga ccgatgcgct
      601 cgcacagcag gggctgggtg cgcctcaagt ccgcggatcc cctggagaag ccgcgggtct
      661 tcttcaatta catgtcgcag ccccaggact gggaagagat gcgcgcctgc gtgcgcctga
      721 cacgcgagat ctttgcgcag ccggccttcg cccgctaccg cggccgcgag atccagccgg
      781 gcgagccggt gcagagcgac gccgagatcg acgcctttat ccgcgccaag gtggaaagcg
      841 ccttccaccc gtcctgcagc tgccggatgg ggcgggcctc ggacccgctc gcggtggtgg
      901 acgcccaggc gcgcgtcatc ggcacgcagg cgctgcgagt cgtcgactcc tcgatcatgc
      961 cctcgatcac cacggggaat ctcaacgcgc cgaccatcat gctggcggag aaggctgccg
     1021 actacatccg cggccgcgcc ctgctgccgc cgtcggctgc tccgtactac gtggcggccg
     1081 actgggagag caagcagcgc tagtcgcggt agcgcgtcag cgcgtccccg agtgccgcac
     1141 gcagcgactc gagcgacggt tcgtagtgcc gccactggtc gagtccctcg cgatagatcg
     1201 gctggcggac ttgctccgag ctcgcggttc tgatgctgcg ctcggtccgg tagaactcga
     1261 cgcacgccgg ctcgaagccg agcccgcaat actcgagcag gcggcgcacg ttgccctcga
     1321 ggtcgttcac caggtcctca tgctgaacgc gcagcacccg acccggcagc acccgctccc
     1381 aatgccgcat gagctcgaga taggtgcggt agtagcgcgc gatgtcctcg atcccgtagg
     1441 tgaattcctg tcccttggcg aacagctgct tgaagttccc gaagcagcag gccatcggct
     1501 cgcgccgcgc atcgacgatc ctggcgttcg gcagcatcag gtggatgagc ccaaggtgcc
     1561 ggaagttgtt cggcatcttg tcgatgaagc gcggcctgcc ggtg
//
LOCUS       DATLKL010000475         2408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_208586, whole genome shotgun sequence.
ACCESSION   DATLKL010000475 DATLKL010000000
VERSION     DATLKL010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208586"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VM713_06355"
     CDS             complement(<1..251)
                     /locus_tag="VM713_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83920.1"
                     /translation="MENQPSVTVNNPYGLSALWVSGDLVSRSVLILLLLMSLASWYVI
                     LTKLWDQRALRKSAKSVDKQFWNAPSIKDGVDRLKKGDD"
     gene            complement(303..929)
                     /locus_tag="VM713_06360"
     CDS             complement(303..929)
                     /locus_tag="VM713_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HVH83921.1"
                     /translation="MATLVHDSQFMTRRGAVLVASIVLHLFLVWALATGLARRAIEVL
                     APPIQTTIVEETKKEEAPPPPPPPQFERPPVELPPPDVTINMPVETETTAIRVTNTPV
                     VKAPPVAVAKPGTPVGFGKGFPNSDEFYPDASRRLEEQGVATVHVCVGPDNKLTEEPS
                     IAKSSGSARLDGGAMKLAKAGSGRYIAATEEGHAITKCINYNVKFQLR"
     gene            complement(1128..>2408)
                     /locus_tag="VM713_06365"
     CDS             complement(1128..>2408)
                     /locus_tag="VM713_06365"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HVH83922.1"
                     /translation="LNRPAVFVYGGTIRPGAQRRDIVTVFEAVGARSAGTLSESGLME
                     IERTAIPGPGSCGGMYTANTMASAIEALGLSLPGSSAQEAVGEAKHSDCARAGAAVVR
                     LVREGLRPRDILSRKAFENAITVVTALGGSTNAVLHLLAIARDAGVRLTLDDFTRVGR
                     RSPVLADLKPSGRYLMSELVAIGGIQPLMKMLLDGGLLHGECLTVTGQTLKQNLEKTA
                     MYMRGQEIVRPLSDPLKKDGHLVILYGNLAPAGAVAKITGKEGERFEGRARVFDGEER
                     ATQAILANKLRAGDVVVIRHEGPKGGPGMREMLSPTGAISGRGLDGKVALITDGRFSG
                     GSHGFVVGHVSPEAAVGGPIGLLHNGDRIIIDARKRELRVELPAAQLRERRRAWQPPR
                     PAAQAGVLAKYARLVGSASEGAVTEAIQARRRNS"
BASE COUNT          379 a          839 c          789 g          401 t
ORIGIN      
        1 aagtcgtcac ccttcttcag gcgatcgacg ccgtccttga tcgaaggcgc gttccagaac
       61 tgcttgtcga ccgacttggc cgacttgcgc agcgcccgct ggtcccacag cttcgtgaga
      121 atcacgtacc acgaagcgag cgacatgagg agcagaagaa tgagcacgga acgggagacc
      181 aggtctcccg acacccacag tgcgctcagg ccgtagggat tgttaacggt tacgctcggt
      241 tggttttcca tagtttcctc gaaacaaaaa agaagccgca ccgtggcagg tgcggccagt
      301 gttcatctca gctggaattt gacgttgtag ttgatgcatt tggtgatcgc gtggccttcc
      361 tcggtcgcgg cgatgtacct gcccgagccc gctttcgcga gcttcatcgc cccgccgtcg
      421 agacgcgcgc tgccggagct cttggcgatc gacggctcct cggtgagctt gttgtcggga
      481 cccacgcaca cgtgcacggt cgcgaccccc tgctcctcga ggcggcgcga ggcgtccggg
      541 tagaactcgt cgctgttggg gaagcccttg ccgaacccga ccggagtccc cggcttggcg
      601 accgcaaccg ggggtgcctt gacgaccggc gtgttagtga cgcggatcgc cgtggtctcc
      661 gtctccaccg gcatgttgat cgtgacgtca ggcggcggca gctccaccgg gggccgctcg
      721 aactgcggcg gcggcggcgg cggcggcgcc tcctctttct tcgtctcctc gacgatcgtc
      781 gtctggatcg gcggggcgag cacctcgatc gcgcggcgcg cgaggcccgt cgccagcgcc
      841 cagacgagga acagatggag cacgatgctc gcgaccagca cggcgccgcg ccgggtcatg
      901 aactgagaat cgtgtactaa ggtggccatg tgttgttgag aaccccgttg ccggcgggcg
      961 tacctgccgg ttccccaaaa tcatacttgc gaactcgctg cgtcagacag ccgcacggcg
     1021 ggcgtgaaat tctaaaatcc cccggtcggc gccccgcaga gtgcgctgcg atgaatgctt
     1081 tcgcgaactg taccgggtgc ccccgggact gacaactccg aagccactca ggaattgcgc
     1141 cgacgcgcct gaatcgcctc ggtgacggcg ccctcggagg cgctgccgac gagccgcgcg
     1201 tacttggcga gcacgccggc ctgcgcggcg ggccgcgggg gctgccacgc ccgccgccgc
     1261 tcgcgcagct gcgcggccgg cagctcgacg cgaagctcgc gcttgcgcgc atcgatgata
     1321 atgcggtcgc cgttatggag cagcccgatc ggaccgccca cggcggcctc cggagacacg
     1381 tgtccgacca cgaatccgtg gctgcccccg gaaaaacggc cgtcggtgat caacgccacc
     1441 ttgccgtcca ggccccgccc gctgatggcg cccgtcgggc tcagcatctc gcgcatgccc
     1501 gggccgccct tcggcccttc gtgccggatc acgacgacgt cgcccgcgcg cagcttgttg
     1561 gccagaatcg cctgcgtggc gcgctcctcg ccgtcgaaga cccgcgcccg gccctcgaag
     1621 cgctccccct ccttgccggt gatcttcgcc actgcgccag cgggcgcgag attgccgtag
     1681 aggatgacga ggtgaccgtc ctttttcagc gggtccgaga gcggccggac aatctcctgc
     1741 cctctcatat acatggccgt cttttcgaga ttctgcttca gggtctgccc ggtgaccgtc
     1801 aggcactccc cgtgcagcag gcccccgtcg agcagcatct tcatgagagg ctggatcccc
     1861 ccgatggcga cgagctcgga catcagatag cggccgctcg gcttgaggtc cgcgagcacc
     1921 ggggaacggc ggccgacgcg ggtgaaatcg tcgagcgtaa ggcgcacgcc cgcgtcgcgg
     1981 gcgatcgcga gcaggtgcag cacggcgttg gtcgagccgc cgagcgcggt caccaccgta
     2041 atggcattct caaaggcctt gcggctcaga atgtcgcgcg gtctcaaccc ctcgcgcacc
     2101 aggcgcacga ctgcggcgcc ggcgcgcgcg cagtcggagt gcttggcctc cccgaccgcc
     2161 tcctgggcgg agctccccgg caatgacaag cccaaggctt cgattgccga agccatcgta
     2221 ttggccgtgt acatgccgcc gcagctgccg gggccgggaa tggccgtgcg ctcgatctcc
     2281 atgagaccgc tctcggacag cgtccccgcc gagcgcgctc cgaccgcctc gaacaccgtc
     2341 acgatgtcgc gccgctgggc gcctggccgg atcgtgcccc cgtagacaaa gaccgccggg
     2401 cggttgag
//
LOCUS       DATLKL010000476         2243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_73525, whole genome shotgun sequence.
ACCESSION   DATLKL010000476 DATLKL010000000
VERSION     DATLKL010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73525"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VM713_06370"
     CDS             complement(<1..1018)
                     /locus_tag="VM713_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HVH83923.1"
                     /translation="MSADTTAREEGALALVAPLSGWSTPLDEAPDAVFAGRILGDGVA
                     IDPTAGTLCAPCDGELLVVAPSRHAVTLRTAGGCQILMHVGIDTVSLGGAGFTLAAQQ
                     GARVRSGDALLHFDLDLLARRAPSLLTPIVITADSGFRIVRRVEGREVKVGDPLMDLA
                     PELVAAATVAAPGPVASQRAIVTFEHGIHARPAALLAASLKNLAADVRLAAGAREANA
                     RSTVSLMALGAQHGDEIEIRAAGPDAALAVGALAAVLGAEAAAPRKNAAAAPPAAAAA
                     PAASSAAPQAGDALAGVIACRGLGVGRAFQLRRPEIAVMAAGAGVVHESEAFDRARAL
                     VHARL"
     gene            complement(1015..>2243)
                     /locus_tag="VM713_06375"
     CDS             complement(1015..>2243)
                     /locus_tag="VM713_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HVH83924.1"
                     /translation="PPAAVPSDWGVYPNLFNVDDSTFTFIENVLDEFMALFPGEYIHT
                     GGDEAVKDQWRASARIQARMRELAVPTEEALQGYFTARVGAYLARHGRRQIGWDEILE
                     GGVPPDAAVMSWRGVEGAIAAAARGHDTVLSPAPTLYLDNRQGPTPAEPPGRGTVITL
                     QDVYRFDPLPGPLAHDQSHVLGVQANLWTEHVRTEADAAYMTWPRAAALAEVGWSSAA
                     RIDWESFEARLAAEFDRYRTAGIRYSDDVFAPLRNVGPYERHFSQDLQTCSAKLVLNL
                     EDDAPLTGPRAVFLVDIMDPCWIFPASDLTHAASITAAVGQVPFNFQIGRDVEAIHFD
                     PPRTAAGELEVRLDRCDGVPLVVLPLAPAAGNEAVTVLPPAALPQQSGAHSLCLRFTQ
                     RTLDPLWTIDWVQLSQ"
BASE COUNT          333 a          792 c          828 g          290 t
ORIGIN      
        1 cgagccgggc gtgcaccagc gcgcgggccc gatcgaaggc ctccgactcg tgcaccactc
       61 ccgcgcccgc tgccatgacg gcgatctccg gccggcgcaa ctggaacgcg cggccgacgc
      121 cgagtccgcg gcacgcgatc acgcccgcga gcgcatcccc tgcctggggg gcggcgctgc
      181 tcgcggccgg ggccgctgcg gcggccggcg gggcggccgc cgcgttcttg cggggcgcag
      241 ccgcctcggc accgagtacg gcggcgagtg ctcccaccgc cagcgcggcg tcgggacccg
      301 cggcgcggat ctcgatctca tcgccgtgct gcgcgccgag tgccatgagc gacacggtgc
      361 tgcgcgcgtt cgcctcgcga gcgcccgcgg cgaggcgcac gtcggcggcg aggttcttca
      421 gtgaggccgc gaggagcgcc gcggggcgcg cgtgaatacc gtgctcgaag gtaacgatcg
      481 cgcgctgcga cgccacgggt ccgggcgcgg cgacggtcgc agccgcgacg agctcgggcg
      541 caagatccat gagcggatcc ccgaccttga cctcgcgccc ctcgacacgc cgcacgatac
      601 gaaagccgct atcggccgtg atgacgatgg gcgtgaggag actcggggcg cgccgtgcga
      661 gcaggtcgag atcgaaatgc agcagcgcat cgccgctgcg cacacgcgcg ccttgctgag
      721 cggccagggt aaagccggcg ccgccgagac tcacggtgtc gattcccacg tgcatgagga
      781 tctggcagcc gcccgcggtg cgcagcgtca ccgcgtggcg cgacggggcg accaccagga
      841 gctcgccgtc gcagggggcg cagagcgtcc cggcggttgg atcgatggcg acgccatcgc
      901 ccaaaatccg cccggcgaac accgcatccg gcgcctcatc gagcggcgtg ctccacccgc
      961 taagcggcgc caccagggcg agcgcaccct cctcccgggc tgtcgtgtcg gcgctcattg
     1021 cgagagctgc acccagtcga tcgtccagag tgggtcgagc gtgcgctgcg tgaagcgcag
     1081 gcacagggag tgcgcgccgc tctgctgggg aagcgccgcg ggcggcagca cggtcactgc
     1141 ctcgttgccc gccgccggcg cgagcggcag caccacgagc ggcacgccgt cgcagcggtc
     1201 gagccgcacc tcgagctccc cggcggccgt gcgcggcgga tcgaagtgaa tggcctcgac
     1261 gtcccggccg atctgaaaat tgaacggtac ctggcccacc gccgcggtga tcgaggctgc
     1321 gtgcgtcagg tcgctcgcgg gaaatatcca acaggggtcc atgatgtcga cgagaaacac
     1381 cgcgcgcggg cccgtgagcg gcgcatcgtc ctcgagattc agcacgagct ttgccgagca
     1441 ggtctggagg tcctggctga agtggcgctc gtaagggccg acgttgcgga gcggcgcgaa
     1501 cacgtcgtcg gagtagcgga tcccggcggt gcgatagcgg tcgaactcgg ccgcgagccg
     1561 cgcctcaaag ctctcccagt cgatcctcgc ggcgctcgac caaccgacct cggcgagcgc
     1621 cgccgcgcgg ggccaggtca tgtaggccgc gtcggcctcg gtgcgcacgt gctcggtcca
     1681 gaggttggcc tgaacgccga gcacatggct ctggtcgtga gcgagcggcc ccggcagcgg
     1741 atcgaagcga tagacgtcct ggagcgtgat caccgtgccg cggcccggcg gctcggccgg
     1801 ggtcggaccc tggcgattgt cgaggtaaag cgtcggcgcc ggcgacagca cggtgtcgtg
     1861 cccgcgggcg gccgcggcga tcgcgccctc gaccccgcgc cacgacatga cggccgcatc
     1921 tggcggcacg ccgccctcga ggatctcgtc ccaaccgatc tgccggcggc cgtgacgcgc
     1981 cagataggcg ccgacgcgtg cggtgaagta gccctgcaag gcctcctcgg tcggcacggc
     2041 gagctcgcgc atgcgcgcct ggatgcgcgc cgaggcgcgc cactggtcct tcaccgcctc
     2101 atcgcccccg gtgtgaatgt actcgccggg gaagagcgcc atgaactcgt cgaggacgtt
     2161 ctcgatgaag gtgaaggtcg aatcatcgac gttgaagagg ttcggataga cgccccagtc
     2221 tgagggcacg gcggcgggcg gct
//
LOCUS       DATLKL010000477         1629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_73531, whole genome shotgun sequence.
ACCESSION   DATLKL010000477 DATLKL010000000
VERSION     DATLKL010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73531"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1629
                     /gene="tkt"
                     /locus_tag="VM713_06380"
     CDS             <1..>1629
                     /gene="tkt"
                     /locus_tag="VM713_06380"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HVH83925.1"
                     /translation="ALSMDAVQRANSGHPGMPMGMADIAQVLWSDFLKYNPGNPGWVN
                     RDRFVLSNGHGSMLLYSVLHLTGYPLTIADLEAFRQLGSRTPGHPEYELPLGIETTTG
                     PLGQGLANAVGMALAEKLLALRFNRPGLSIVDHHTYAFCGDGCLMEGVSHEACSLAGT
                     LGLGKLIVFYDDNGISIDGRVAGWFTDDTPGRFAAYGWHVIPGVDGLDPEAVTVAIRA
                     ARAEVSRPSLICCKTIIGYGAPHRQGTKEAHGEALGPEEVAAARVALGWPYPPFVVPE
                     EIRAAWDHRAQGEALEAEWRGLLERYAQQYPDEAREFERRTRGDLPADFRATATQALD
                     TFARQTAAQATRASSQAVLNVFAPALPELFGGSADLTQSNSTFFKGARTITPQDETGD
                     YLHYGVREFGMTAVMNGIALHGGLLPYGGTFLVFSDYARNAVRLASLMQLRVVLVYSH
                     NSIGLGEDGPTHQPIEHLASLRAMPHLSLWRPCDAAETAVAWTLAAERRGPTALVLTR
                     QGLPQQPRTPQQLGSIARGGYILIERPGTPEALVI"
BASE COUNT          247 a          588 c          542 g          252 t
ORIGIN      
        1 gcactctcca tggacgccgt gcagcgtgcg aattccggcc acccgggcat gccgatggga
       61 atggcggata tcgctcaggt gctgtggtcg gatttcctca agtacaaccc gggaaatccc
      121 ggatgggtga accgcgaccg cttcgtgctc tcgaacggcc acggctcgat gctgctatat
      181 tccgtactgc atctcaccgg ctatccgctc accatcgcgg acctcgaggc cttccgccag
      241 ctcggcagcc ggacacccgg ccatccggaa tacgagctgc ccctcggcat cgagaccacc
      301 accgggccac tcgggcaggg actcgcgaac gcggtcggca tggcgctcgc ggagaaactg
      361 ctggcgctgc gcttcaatcg tcccgggctc tcgatcgtcg atcaccatac gtatgccttc
      421 tgcggcgacg gctgtctcat ggagggcgtc tcgcacgaag cctgctcgct cgccggcacg
      481 ctgggcctcg gcaagctcat cgtcttctac gatgacaacg gcatatccat agacggcagg
      541 gtcgccgggt ggttcaccga cgacacgccg ggacgtttcg ccgcctacgg ctggcacgtg
      601 atcccgggcg tcgacggact cgatccggaa gcggtgaccg ttgcgatccg tgccgcgcgc
      661 gcggaagtgt cgcgaccgtc cctcatctgc tgcaagacca tcatcggcta cggcgcgccc
      721 cacaggcagg gcaccaagga ggcgcacggc gaggctctcg ggccggagga agtcgcggcg
      781 gcgcgcgtcg ccctcggctg gccgtatccg ccgttcgtcg tgccggagga gatccgcgcg
      841 gcgtgggatc atcgcgcgca gggggaggcg ctcgaagccg agtggcgcgg gctccttgag
      901 cgctatgcgc agcagtaccc cgacgaggcg cgcgagttcg agcggcgtac gcgcggggat
      961 ctgccggccg acttccgcgc caccgccacg caggcgctcg acaccttcgc ccgtcagacg
     1021 gcggcccagg ccacgcgcgc ctcctcgcag gcggtgctca acgtcttcgc cccggcgctg
     1081 ccggagctgt tcggcggctc ggccgatctc acccagtcga acagcacctt cttcaagggc
     1141 gcgcgcacca tcacaccgca ggatgagacg ggcgactacc tgcactacgg cgtgcgcgag
     1201 ttcggcatga cggccgtcat gaacggcatc gcgctgcacg gcgggctcct gccctatggc
     1261 gggaccttcc tcgtgttctc cgattacgcg cgcaacgccg tgcggctcgc ctcgctcatg
     1321 cagctgcgcg tggtgctggt gtactcgcac aattcgatcg gcctcggtga ggacggtccg
     1381 acgcaccagc ccatcgagca tctcgcgagc ctccgggcca tgccgcacct gagtctgtgg
     1441 cgcccgtgcg atgcggcgga gaccgcggtc gcctggacgc tcgccgccga gcggcgcgga
     1501 cccacggcgc tcgtgctcac gcggcagggc ctgccgcagc agccgcgaac gccccagcag
     1561 ctcggcagca tcgcccgcgg gggctacata ctcatcgagc gcccggggac cccggaagcg
     1621 ctcgtgatc
//
LOCUS       DATLKL010000478         1797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_95975, whole genome shotgun sequence.
ACCESSION   DATLKL010000478 DATLKL010000000
VERSION     DATLKL010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95975"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="VM713_06385"
     CDS             complement(<1..1746)
                     /locus_tag="VM713_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HVH83926.1"
                     /translation="MFAKRSSRALALLLALPSAAFALGLGDIHLNSQLNSPLDAEIEV
                     TDVAPDEAPTVRAQLAAREIFSQNGLDYPTYLTSVQLRTVETTDGRHLIKVKSSDPII
                     DPFITLLINVTWAHGQLVREYTMLLDPPVYTPTNQAVAASAPVAAPVTGAETRAGAIA
                     RAAETAAAAAPAAPAVAPPAPPAPPAPPPSAEPPAAEASQPAPTPTEAAPPPPPPPPP
                     APPPPARPPTKATPAPEGPSASTAPPAPAPAQAANTDHRVEHGETLAGIARGLTGAAA
                     NSARTRAWILAIYEANPKAFDKNMNIMRAGAVLRIPDEGTAEAISPVDATAEISRQFA
                     AWRSSTAPSAGGTEGATAAPSAQPGRLHLVTPSEGASAGAGTAANSAETTALKGRVQD
                     LEGQLAEQKRLLDLKNQELARLQSELAAKQQAAKAPTPAPPPAPAPVVQAAPPPPAPA
                     PEEKPAAKEEQPPPVAAAPPPPVTKPLAKAAPAEKPEPAPAEGGSILDTIMGYWWLLA
                     LVAVVLAAVAGLRFMRSRRAAEFDDSLGRLAVAAVAGGNAAGSALGGAGARGAGFDAP
                     AMRAMPLSEPEPSVVV"
BASE COUNT          224 a          597 c          699 g          277 t
ORIGIN      
        1 gacgacgacg ctcggctcgg gctcgctgag cggcatggcg cgcatggctg gcgcatcgaa
       61 ccccgccccg cgcgcccctg ctccgccgag cgcactaccg gcggcgttcc ctccggcgac
      121 ggcggccacg gccagccggc cgagcgagtc atcgaattct gcggcccggc gcgagcgcat
      181 gaacctgagg cccgctactg ccgcgagcac caccgcaacc agcgcgagca gccaccagta
      241 gcccatgatc gtatcgagga tcgagccgcc ctcggcgggg gcgggctcag gcttttccgc
      301 gggcgcagcc ttcgccaacg gcttcgtgac cggcggcggc ggcgcagcgg cgaccggagg
      361 cggctgctct tccttcgcag ccggcttctc ctcgggagca ggcgccggcg gcgggggcgc
      421 ggcctggacg accggtgctg gggccggcgg cggggccggc gtgggcgctt tggcggcctg
      481 ctgcttcgcg gcgagctccg actggagccg cgcgagttcc tgattcttga gatcgaggag
      541 gcgcttctgc tcggcgagct gcccttcgag atcctgcacg cggcccttga gcgcggtcgt
      601 ctcggcgctg ttggccgcgg tgccggcgcc cgccgaggcg ccctctgatg gcgtgacgag
      661 atgcagtcgc ccgggctgcg cgcttggcgc ggcagtagcg ccctcggtgc cacccgcgga
      721 cggtgcggtg ctcgagcgcc aggccgcgaa ctggcggctg atctcggcag tggcgtcgac
      781 gggcgagatt gcctccgccg ttccctcgtc cggaatgcgc agcaccgcgc ccgcgcgcat
      841 gatgttcatg ttcttgtcga aggccttggg gttcgcctca tagatggcga gtatccaggc
      901 gcgcgtgcgc gcggaattcg ccgcagctcc ggtcagcccg cgcgcgatcc ccgcgagcgt
      961 ctcgccgtgc tccacccggt ggtccgtgtt ggcggcctgt gcgggggcgg gggctggagg
     1021 cgccgtgctc gcactcggac cttcaggggc cggagtggcc ttggtcggcg ggcgcgccgg
     1081 tggcggcggt gccggcgggg gtggcggggg tggcgggggc gccgcctcgg tgggtgtggg
     1141 cgcaggctgc gacgcctctg cggctggagg ctcagcgctc ggcggcggcg cgggaggtgc
     1201 tggaggtgcc ggaggcgcca ccgcgggagc cgcgggagcc gcggctgccg ctgtctcggc
     1261 agcgcgcgca atcgcgccgg cgcgcgtctc ggcaccggtc accggcgcgg cgaccggcgc
     1321 gctcgctgcc accgcctggt tcgtcggcgt gtacaccggc ggatcgagca gcatggtgta
     1381 ctcgcgcacc agctgtccgt gcgcccaggt gacgttgatg aggagcgtga tgaaaggatc
     1441 gatgatcggg tccgaggact tgaccttgat gaggtggcgg ccgtcggtcg tctccaccgt
     1501 gcgcaactga acgcttgtca ggtaggtcgg atagtcgagg ccgttctgcg agaagatctc
     1561 gcgcgccgcc agctgcgcac gcacggtggg ggcttcgtcg ggtgcaacgt ccgtgacctc
     1621 gatctcggca tcgagcgggg agttgagctg cgagttgagg tgaatgtctc cgagacctag
     1681 ggcaaacgcg gcggacggca aggccagcag caatgccagc gcccggcttg agcgtttggc
     1741 gaacatcagg acaccccggc aggagccatt agaaatatct tgtagaaaca atcggat
//
LOCUS       DATLKL010000479         1599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_208599, whole genome shotgun sequence.
ACCESSION   DATLKL010000479 DATLKL010000000
VERSION     DATLKL010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208599"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..>1599
                     /locus_tag="VM713_06390"
     CDS             101..>1599
                     /locus_tag="VM713_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83927.1"
                     /translation="MRVGVLELLTDSQNRGYTAALYARYYRRQFMSIMPQVVAVWCRQ
                     LGHRVSYATYCGNGDPRSLLPDDLDVVFIAVYTQASALAYALAKLYRRAGVLTVIGGP
                     HAKCFPADCLRFFDVVVKDCDRALIDDILRGRVDAPAIVTSGRSLASLPSVEERMPEI
                     AASAFTNGRPTLTSLVPLLASVGCPYSCGFCIDWNTNYVPMPRDQLQADLAYLARNYP
                     GVLVGYHDPNFAVRFDETMDVIETIPSERRNRYIMESSLSVLKPARLHRLRATRCVYV
                     APGIESWADFSDKSAVGAKSGRSKLDQVVAHLSEIRRHVPGLQANFIFGTDADHGEEP
                     VELTKEFIRRLPFVFPAVNIPTPFGGTPLFDHHLAEGRILRAMPFAFYYNPYLVTTLP
                     HYGPVEYYRHLIDIHASITSTASLVRRLSAGAPPAMHLVHTLRTFAVASDLGEMRWLY
                     RQLIDDRAFRAFHEGRSDRLPDYYQWRFRERLGVFADQLSWDDLIPRLDA"
BASE COUNT          255 a          573 c          469 g          302 t
ORIGIN      
        1 gttcgatgtt cccgtggcac cgagcagtct catgccggtg caatcgactg ccacggccgc
       61 gatcgatcgc aagcgaggcc gaaatggaga tccgtcaacg atgcgcgtag gcgtcttgga
      121 gctgctgacc gactcgcaga atcgcggcta cacggccgcg ctctacgccc ggtattaccg
      181 ccgccagttc atgagcatca tgccgcaggt ggtggccgtg tggtgccgac agctcggtca
      241 tcgggtatct tatgccacgt actgcggcaa cggcgacccg cgctccttgc tccccgacga
      301 tctcgacgtc gtcttcattg ccgtctacac gcaggcgagc gccctcgcct atgcgctggc
      361 caagctctat cgccgcgccg gcgtgctcac cgtgatcggc ggaccgcacg ccaagtgctt
      421 tcccgccgac tgcttgcgtt tcttcgatgt agtggtcaag gactgcgatc gcgcactcat
      481 cgacgacatt ctgcgcggcc gcgtcgacgc gccggcgatc gtcaccagcg ggcggtcgct
      541 cgcctcgctt ccctccgtcg aggagcggat gcccgaaatc gctgcctcgg ccttcaccaa
      601 cggccgtccg accctcacca gcctggtgcc actgctcgcc agcgtgggct gcccctacag
      661 ctgcggcttc tgcatcgact ggaacacgaa ctacgtgccg atgccgcgcg accagctcca
      721 ggccgacctg gcgtatctcg cgaggaacta ccctggcgtg ctggtcggct atcacgatcc
      781 caacttcgcg gtgcggttcg acgagaccat ggacgtgatc gagacgatcc cgtccgagcg
      841 ccgcaaccgc tacatcatgg agagctcgct ctcggtcctg aagccggcgc gcttgcaccg
      901 cttgcgcgcg acgcgttgcg tatacgtggc gcccggaatc gagtcctggg cggacttctc
      961 agacaaatcg gccgtcggcg cgaaatcggg acgcagcaag ctcgaccagg tggtcgcgca
     1021 cctctccgag atccgacgcc atgtcccggg gctgcaggct aacttcatct tcggcaccga
     1081 cgccgaccac ggagaggagc cggtcgagct gacgaaggag ttcatccgcc ggttgccctt
     1141 cgtctttccc gcggtgaaca tcccgacgcc cttcggcggc acgccgctgt tcgaccacca
     1201 tctcgccgag ggccggattc tccgagccat gccgttcgcg ttctattaca atccgtatct
     1261 tgtcaccacg ctgccgcact acggtcccgt cgagtactac cggcacctga tcgatatcca
     1321 cgcatccatt acctcgaccg cgagtctggt ccgccggctc tccgccggtg cgccgccggc
     1381 catgcatctc gtccacacgc tgcgcacctt cgcggtcgcc agcgacctcg gcgagatgcg
     1441 gtggctctat cgccaattaa tcgacgaccg ggcgtttcgc gcctttcacg agggcagatc
     1501 ggaccggctg cccgactact accaatggcg atttcgcgag cgtctcggcg tcttcgccga
     1561 ccagctttcg tgggacgacc tcatccctag gctcgatgc
//
LOCUS       DATLKL010000480         1867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_186200, whole genome shotgun sequence.
ACCESSION   DATLKL010000480 DATLKL010000000
VERSION     DATLKL010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186200"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VM713_06395"
     CDS             complement(<1..216)
                     /locus_tag="VM713_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HVH83928.1"
                     /translation="MIGAVRGRIASKTPPQLTVDVGGLGYELEAPMSTFFHLPPVGAE
                     VSLLTHLVVREDAHVLYAFATEEERRLF"
     gene            complement(213..809)
                     /gene="ruvC"
                     /locus_tag="VM713_06400"
     CDS             complement(213..809)
                     /gene="ruvC"
                     /locus_tag="VM713_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HVH83929.1"
                     /translation="MSRYRTRSWRAFDPAVAAAAPPAAAPNVRILGLDPGSRRTGFGV
                     LECRGAQLTLLAQGCLNVSAAAPAVRLRLIFEGLAALCRDHDPGEVAVERVFVSRNVD
                     SALKLGQARGAALCAVPGGVPVFEYAPRAIKLALVGSGAAEKFQVAHMVRVLLSVQGP
                     LSADAADALAVAVCHAHTRRLSALVAQQGATRVRQAQR"
     gene            complement(1030..1626)
                     /locus_tag="VM713_06405"
     CDS             complement(1030..1626)
                     /locus_tag="VM713_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVH83930.1"
                     /translation="MGGHLSWASIQRHKGSLAARRRRQRERAPRAGQQLVRFEGYGPG
                     GAAVIVECLTDDRGRVAAQVRRAFLEHGGSLGAEGSVSYLFNTVGLMTYPAGTDEEGL
                     MQVALEAGAEDVVPNGDRSMEVLADPAELDTVRAVLTHRGFAPQSAEVTERAASALAL
                     SGESAELMVRLLEALQGLDEVRDVYSNVEIAAEVLGTL"
     gene            1660..>1867
                     /locus_tag="VM713_06410"
     CDS             1660..>1867
                     /locus_tag="VM713_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83931.1"
                     /translation="METNQLCPPALRDAIIGARAALERAGPRAPADLGFATEAAEIMG
                     GLTENTELANALLARPVISRAALTP"
BASE COUNT          278 a          715 c          623 g          251 t
ORIGIN      
        1 gaacagccgc cgctcctcct cggtggcaaa ggcatacagc acgtgcgcgt cctcgcgcac
       61 gacgagatgg gtcagcaggc tcacctcggc gccgaccggc ggcaggtgga agaaggtcga
      121 catgggtgcc tcgagctcgt acccgagacc gccgacgtcg accgtgagtt gcgggggagt
      181 cttggaggcg atcctgccgc gcaccgcgcc gatcatcgct gcgcctgccg cacgcgcgtc
      241 gcgccctgct gcgcgacgag tgcgctgagg cgccgcgtgt gcgcgtggca cacagctacc
      301 gccagcgcat cggcggcgtc cgccgacagc ggcccctgga cactgagcag cacgcgcacc
      361 atgtgggcca cctgaaactt ctccgccgcc cccgagccga ccagcgccag cttgatcgcc
      421 cgcggcgcgt actcgaacac cggcacgccg cccggcaccg cgcacagcgc cgcgccacgc
      481 gcctgcccga gcttgagcgc gctgtcgacg ttgcggctga cgaacacgcg ctcgaccgcg
      541 acctcgccgg ggtcgtgatc gcggcagagc gccgcgaggc cctcgaagat caggcgcaac
      601 cgcaccgcgg gcgccgctgc actcacgttg aggcagccct gtgcgaggag cgtcagctgc
      661 gcgccgcggc attccagcac accgaaaccg gtgcggcgcg agccgggatc cagaccgagg
      721 atgcggacgt tgggcgccgc ggcgggtggc gcggccgcgg cgactgccgg atcaaacgcg
      781 cgccacgacc tcgtccggta cctcgacatt cggatgaaat tcctccgcgc ctgcaagcct
      841 aactgaaaac aagggcttgc ggaattgtga cgggctgcgc gctctgcgcg ctgaagcgcg
      901 gcggcggcgg atagctggat tgccaccgcg attgcttccc gaaccgcgct cgcggtgctc
      961 ctgagccaca cccgctggac ccccgccggt gggctctttt ttttcccagc ggtcgcgcag
     1021 cgcacgggct cacagcgtac cgagcacctc cgcggcgatt tcgacattgg aatagacgtc
     1081 gcgcacctca tcgaggccct gcagcgcttc gagcagtcgc accatgagct cggcggactc
     1141 gcccgacagc gcgagcgcgc tggcggcacg ctcggtcacc tcggcgcttt gcggcgcgaa
     1201 gccgcgatgc gtgagcaccg cgcgcaccgt atcgagctcc gccgggtccg ccagcacttc
     1261 catggagcgg tcgccgttcg gcaccacgtc ctcggcgccc gcctcgagcg ccacctgcat
     1321 gagcccctcc tcgtcggtcc ccgccgggta ggtcatgagg ccgacggtgt tgaagaggta
     1381 actcaccgag ccctcggccc cgaggctacc gccgtgctcg aggaaagcgc ggcgcacctg
     1441 cgctgcgact cgtccgcggt cgtcggtcag gcactcgacg atcacggcgg cgccgcccgg
     1501 gccatagccc tcgaagcgca ccagctgctg tcccgcacgc ggcgcgcgct cgcgttgccg
     1561 gcggcgccgg gccgcgaggc tccccttgtg gcgttggatg ctggcccagc tcaggtgtcc
     1621 gcccatgcgt ttatgatagc cacacgcccg cggactcaca tggaaaccaa ccaactctgc
     1681 ccacccgcgc tgcgcgatgc catcatcggc gcgcgcgccg ccctggagcg cgccggcccg
     1741 cgcgcgccgg cggacctcgg cttcgccacc gaggccgccg agatcatggg cggtctcacc
     1801 gagaacaccg agctcgccaa cgcgctgctc gcgcgcccgg taatctcgcg cgccgcactc
     1861 acgcccg
//
LOCUS       DATLKL010000481         1587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_186204, whole genome shotgun sequence.
ACCESSION   DATLKL010000481 DATLKL010000000
VERSION     DATLKL010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186204"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..846
                     /gene="mlaE"
                     /locus_tag="VM713_06415"
     CDS             82..846
                     /gene="mlaE"
                     /locus_tag="VM713_06415"
                     /inference="COORDINATES: protein motif:HMM:NF033619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HVH83932.1"
                     /translation="MNPRRPGTYLIGGVRRLGSVTIFLVQLLIQCVPALARPRLLVMQ
                     IYNAGARSLIIIMLSGLFVGMVLGLQGYDLLRRFGSEEALGIAAALGLLRELAPVITA
                     LLFAGRAGTALASEIGLMRATDQLTAMEIMAVDPLRYVAAPRFLGGVIAMPLLTAIFS
                     VIGLYGAQLVGVQLMGVDGGVFWSQTQGSVGLHDVNEGLMKSVVFGIACSLIAVHGGY
                     HAEPTAEGVGLATTRTVVASSVIILLLDYVLTAAFL"
     gene            862..1401
                     /gene="mlaD"
                     /locus_tag="VM713_06420"
     CDS             862..1401
                     /gene="mlaD"
                     /locus_tag="VM713_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HVH83933.1"
                     /translation="MQSNRTLEIGTGLFVLLGFAALLFLTTQLPASGVKFAGQKAGYH
                     VTAEFDNVGDLKIGSPVTMSGVRVGEVSGIRFNSRVYKAVVSMRIDPQYNEIPEDSFA
                     SIQTEGLLGGKYVGISPGGLDSYLKDGSRIDQTQSAIVLESLINKFFAAFASKGSGES
                     DGQGTDSQGKGTGKMEPKK"
     gene            1398..>1587
                     /locus_tag="VM713_06425"
     CDS             1398..>1587
                     /locus_tag="VM713_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83934.1"
                     /translation="MRIASVVLTAVSLCAAAMAGAHGAPAAAGQAAAPNAAAGTAASA
                     DSSDPTALVQTTAQGMLGE"
BASE COUNT          255 a          506 c          547 g          279 t
ORIGIN      
        1 gaccggcagc gccgaaggcc cggtgccgtt tcactatccc gccccggatt acgccacgca
       61 gcttctcgag gcggacgagc gatgaatccc cggcgccccg gcacctacct cattgggggc
      121 gtgcgccgcc tcgggtcagt caccatcttc ctggtgcagc tcctcatcca gtgtgtgccc
      181 gcgctcgcgc ggccgcgcct gctggtgatg cagatctaca atgccggcgc ccgctcgctc
      241 atcatcatca tgctgtcggg tctgttcgtc ggcatggtgc tgggtctgca gggctacgat
      301 ctgctgcggc gcttcggctc cgaggaggca ctcggcatcg cggcagccct cggcctcttg
      361 cgcgagctcg cgccggtgat cacggcgttg ttgttcgcag gccgcgccgg caccgcgctc
      421 gcctcggaaa taggcctgat gcgcgccacc gaccagctga cggcgatgga gatcatggcg
      481 gtcgatccgc tgcgctacgt ggcggcgccg cgcttcctcg gcggagtgat tgcgatgccg
      541 ctgctcaccg ccatcttcag cgtgatcggg ctctatggcg cgcaactcgt cggcgtgcaa
      601 ctgatggggg tcgacggcgg cgtcttctgg tcgcagactc agggctccgt cggcctgcac
      661 gatgtgaacg aaggcctgat gaagagcgtc gtattcggga tcgcctgcag cctgatcgcg
      721 gtgcacgggg gttatcacgc ggagcccacg gccgaggggg tggggctcgc gaccacccgc
      781 acggtcgtgg catcgtccgt gatcatcctg ttgctcgact acgtcctcac cgccgccttc
      841 ctctgagagg aactaccagc catgcaaagc aatcgaaccc tggagatcgg tacgggcctg
      901 ttcgtgctgt tgggattcgc ggcgctgctt tttctcacga cgcagctgcc ggcgagcggc
      961 gtcaagttcg cggggcagaa agcgggttat cacgtgaccg cggaattcga caacgtcggc
     1021 gacctcaaga tcggatcgcc cgttaccatg tcgggcgtgc gcgtcggcga ggtgtccggc
     1081 atccgtttca attccagggt gtacaaggcg gtggtgagca tgcgcatcga tccgcagtac
     1141 aacgagatcc ccgaagacag cttcgccagc atccagaccg agggactcct cggcggcaag
     1201 tatgtcggca tcagccccgg gggactcgac agctatctca aggacggcag ccgcatcgat
     1261 cagacccagt cggcgatcgt gctcgagagt ctcatcaaca agttcttcgc ggcctttgcc
     1321 agcaaaggca gcggtgagag cgacggccag ggtactgaca gccagggcaa gggcacgggc
     1381 aagatggagc ctaagaaatg agaatcgcaa gtgtggtgtt gacggcggtc tcgctgtgcg
     1441 cggcggcgat ggctggggcg cacggcgcgc cggccgcggc gggtcaggcg gcggcgccga
     1501 atgcggccgc ggggaccgcg gcgtctgccg acagcagtga ccccacggcg ctggtgcaga
     1561 ccacggcgca ggggatgttg ggtgagc
//
LOCUS       DATLKL010000482         2375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_73542, whole genome shotgun sequence.
ACCESSION   DATLKL010000482 DATLKL010000000
VERSION     DATLKL010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73542"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VM713_06430"
     CDS             complement(<1..271)
                     /locus_tag="VM713_06430"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="TrpG; with TrpE catalyzes the formation of
                     anthranilate and glutamate from chorismate and glutamine;
                     TrpG provides the glutamine amidotransferase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate/aminodeoxychorismate synthase
                     component II"
                     /protein_id="HVH83935.1"
                     /translation="MRPRLLLIDNYDSFAYNLVQAFLVLGAEVDVHRNDALTPVAARA
                     LAPSHLCISPGPGTPYDAGVSMDMIRAFAGSIPVLGVCLGHQAIVE"
     gene            complement(271..1656)
                     /locus_tag="VM713_06435"
     CDS             complement(271..1656)
                     /locus_tag="VM713_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HVH83936.1"
                     /translation="MQTAFDITGDLDTPVSAFMKLRAFAPRFLLESVEGGERLGRYSF
                     VGFGDGLELRLDQAGLAIGGERRGLPADGAELLAGLRAGLAAAPQPQPDIPGVPLAGG
                     LVGYAAYDVVRYFERLPARARTDHGVPALHYVAPRSVLVFDHLTRGIALLHAGDEAER
                     RALKREVVQALRGALPNGRRPGRYSPPAAAYTREAYMAGVRRTQEYIAAGDVYQMVLA
                     SRFAGRHELDPFQVYRALRLINPSPYMYYCALGEVAVVGSSPEALVKLSGRRAQLRPI
                     AGTRPRAADAAVDAAREAELRADPKENAEHVMLVDLARNDLGRVACAGSVAVDPYRAI
                     ERYSHVMHMVSGVHGELAPGRDAFDLFAAAFPAGTLVGAPKVRAMQIIDELEPVRRGL
                     YGGTVGYFGARGDMDHAITIRTLVFSGDEYSYQAGAGIVADSVPGTEHEEVLAKSAAM
                     VRALELAEEGL"
     gene            complement(1963..2325)
                     /locus_tag="VM713_06440"
     CDS             complement(1963..2325)
                     /locus_tag="VM713_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVH83937.1"
                     /translation="MYNHIGLKVRDLDASVRFYTAALKPLGHVLAFADESTAGFGPEG
                     APAFWLYADAKLAHSLLHIAFDANTRTAVDAFYREGLKSRGRDNGGPGIRKDYAPNYY
                     AAFLFDPDGNNVEAVCLK"
BASE COUNT          382 a          809 c          844 g          340 t
ORIGIN      
        1 cctcgacgat cgcctggtgc ccgaggcaca cgccgagcac cggaatgctg ccggcgaagg
       61 cgcggatcat gtccatggac acgccggcat cgtacggggt gccggggccc ggcgagatgc
      121 acagatgcga gggcgccagc gcgcgcgccg cgaccggagt cagcgcgtcg ttgcgatgca
      181 cgtccacttc ggcgccgagc acgaggaacg cctgcaccag gttgtaggcg aaggagtcgt
      241 agttgtcgat gagcagcagg cgcggcctca ttacaggccc tcctccgcga gctccagcgc
      301 ccgcaccatc gccgcgctct tggcgagcac ttcctcgtgc tcggtgcccg gcacgctgtc
      361 ggcgacgatg ccggcgcccg cctggtagct gtactcatcg cccgagaaca cgagcgtgcg
      421 gatggtgatg gcgtggtcca tgtcgccgcg cgcgccgaag tagccgaccg tgccgccgta
      481 gaggccgcgg cgcaccggct cgagctcgtc gatgatctgc atggctcgca ccttcggggc
      541 cccgacgagc gtcccggccg ggaacgccgc cgcaaacagg tcgaaggcat cgcgcccggg
      601 ggcgagctcg ccgtgcacgc cgctcaccat gtgcatgacg tggctgtagc gctcgatggc
      661 acggtaggga tccaccgcga cgctgcccgc gcaggcgacc cggccgaggt cgttgcgggc
      721 gagatcgacc agcattacgt gctcggcgtt ctccttggga tcggcgcgca gctctgcctc
      781 gcgcgccgca tcgaccgcgg cgtcggcggc gcgcgggcgg gtgccggcga tgggacgcag
      841 ctgggcgcgg cggccggaga gcttcaccag tgcctcgggg gaggagccca ccaccgccac
      901 ctcgccgagt gcgcagtagt acatataagg agaggggttg atgaggcgca gcgcgcgata
      961 cacctggaac gggtcgagct cgtggcggcc cgcgaagcgg ctcgccagca ccatctggta
     1021 cacatcgccc gcagcgatgt attcctgcgt gcgccgcacg cccgccatgt aggcttcgcg
     1081 ggtgtaggcc gcggccggcg gggagtagcg ccccggacgg cggccgttcg gcagcgcgcc
     1141 gcgcagcgcc tgcaccacct cgcgcttgag cgcgcgccgc tcggcctcgt cgcccgcatg
     1201 cagcagcgcg ataccgcgcg tcaggtgatc gaacaccagt acggagcggg gcgccacgta
     1261 gtgcagcgcc ggtaccccgt ggtcggtacg ggcgcgcgcc ggcagccgct cgaagtaacg
     1321 caccacgtcg taggcggcgt agccgaccag tccgccggcg agcggcaccc cggggatgtc
     1381 gggctgcggc tgcggcgcgg cggcgagccc ggcgcgcaga cccgcaagca gctccgcgcc
     1441 gtcggccggg agtccgcggc gctcgccccc gatcgcgagc cccgcctggt cgagccgcag
     1501 ctcgagcccg tcgccgaagc cgacgaagga gtagcgcccg agccgctcgc cgccttcgac
     1561 gctctcgagg aggaagcgcg gcgcgaacgc gcggagcttc atgaacgccg agaccggcgt
     1621 gtcgaggtca ccggtgatgt cgaaggcggt ctgcaagaat cgaggctccg agtatttaag
     1681 cgaagacaac aaaaaaccca tcccggggtc atccggagat gggttgctgg tgccgttaga
     1741 tcgtttaagt cgcggggaaa aagctaaccg tcagcccact ccgttatgaa gtgcgccacc
     1801 accaggaagt gctgacgggc cgctgcgaca tgctgcgaac gagtatccct gcttcgggcg
     1861 cgaggcgtca agctcggccc ggcgggcggt cgaggcgttc ccgcgcagtg ctgtcagcca
     1921 cgcgcctcgc ggcgcgtcag ccagcgcgtg atcactacat cgctacttca gacataccgc
     1981 ctcgacgttg ttgccgtcgg ggtcgaagag gaacgcggca tagtagttcg gggcgtagtc
     2041 cttgcggatg ccgggtccgc cattgtcgcg cccgcggctt ttcagcccct cgcggtagaa
     2101 ggcgtcgacc gcggtccgtg tattggcatc gaaggcgatg tgcagcagcg agtgggcgag
     2161 cttggcatcg gcatagagcc agaaggccgg cgccccctcg gggccgaagc cagccgtcga
     2221 ctcatcggca aatgccagga cgtggccgag cggcttaagc gccgccgtgt agaagcgcac
     2281 gctcgcgtcg agatccctca ccttcaggcc aatatggttg tacatgatcg atctcccctt
     2341 aaatcagggc cgatgatgaa ctcgggcccg gcctt
//
LOCUS       DATLKL010000483         3259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_152487, whole genome shotgun sequence.
ACCESSION   DATLKL010000483 DATLKL010000000
VERSION     DATLKL010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152487"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..932
                     /locus_tag="VM713_06445"
     CDS             144..932
                     /locus_tag="VM713_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVH83938.1"
                     /translation="MARAPAPAPVAAPVAAEHPAPAPSAASLPLDLVPFTKLRAGGQT
                     RRFQVQPRAPLAQRNAAAFDEQTLQHLLEWLAANRAEWNGVPTSFTVNLSIATLEDER
                     FLQRIGAALQTHGIAGDTLGFEIAESLCAQQRAKVERFFSQCEKLGTWIAIDDFSFDS
                     QVLPLLRSRALRLLKLDARLTAAALRDKLSQAMVVATVQAAKVLGIHCSAKKADSHAS
                     LQYLAAIGLDYAQGAALSKAVPLEDIATVADPRPSAIPHLGNNE"
     gene            1103..2233
                     /locus_tag="VM713_06450"
     CDS             1103..2233
                     /locus_tag="VM713_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVH83939.1"
                     /translation="MPTLEQFRKNRDVLSWTLHGAFWAAYGITQYVGDFFYAYGSSSS
                     WGPLLLIAAVAGFVLSAPMRYIYRRLWGQQPRAIIVGVLVTCYITALALRLVINLFYR
                     ELIAPDWEVKTLFELFGGALSTAYLLLCWSVLYFGIKFYESKRQQEEAMLKAVALAQE
                     AQLKMLRYQLNPHFLFNTLNAISTLILDNQNRKANQAVARLSEFLRYTLDQDPVKKVT
                     LRQEIEALDLYLGTERLRFGERLKLEYAIEEPALDALVPSLLLQPLLENSLKYAVSAR
                     EQGGMVRIEGRTREGLLELSVIDDGPGLPEGPPPGDRRGVGLANTRERLKVLYGESCR
                     FAALNTHPGLRVDMALPLETAPPEERAAQRALSARLSGGVHA"
     gene            2230..3051
                     /locus_tag="VM713_06455"
     CDS             2230..3051
                     /locus_tag="VM713_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HVH83940.1"
                     /translation="MKVRTIIVDDEVLSRRGIEIRTRELPDFEIVAQCANGREALAAI
                     QQYKPDLVFLDIQMPAMSGFEVLAHLPQESFPLVVFVTAYDQYAIQAFEARAIDYLLK
                     PIDEVRFAEAVARVREHLRARSAAAQRDRLMQIIAEITGCGELALDELLEHGRKALSG
                     GRPEVLPIRQGRETVRVPIASIQWVDAAGDYMCIHAAGETHILRGTMKELEELLDPKL
                     FQRVHRSTIVNLRLVRSLRAHMNGEYFLTLESGHELKLSRTYRDKIEYFLSGSMS"
BASE COUNT          511 a         1133 c         1103 g          512 t
ORIGIN      
        1 cgcagccccc acgcgcagcg gctgcgccgc gcagcgtgcc gcccccgcat tccgcgcccg
       61 ccgtccgcac gccgactgcg gctcgccccg cacctgccgc acctgccgca cctgccgcgc
      121 cggtcgcccc gcgatccgta cccgtggctc gcgcgccggc tccggcgccg gtcgcggcac
      181 ccgtggcggc ggagcacccg gcccccgcac cgagcgcggc gagtctgccg ctcgatctcg
      241 taccgttcac gaagctgcgc gccggcggcc agacgcgtcg cttccaggtg cagccgcgcg
      301 cgcctctcgc gcaacgcaat gccgcggcgt tcgacgagca gaccctgcag catctgctcg
      361 agtggctggc ggccaatcgc gcggagtgga acggcgtgcc gacgagcttc acggtcaatc
      421 tctcgatcgc cacactcgag gacgagcgct tcctgcagag gatcggcgcg gcgcttcaga
      481 cccacggcat cgcgggcgac actttaggat tcgagatcgc cgagtcgctg tgcgcgcagc
      541 agcgcgccaa ggtcgagcgc ttcttttccc agtgcgagaa gctcggcacc tggatcgcga
      601 tcgacgactt ctcattcgat tcccaagtgc tgccgctgct gcgctcgcgg gcgctgcggc
      661 tgttgaagct cgacgcgcgg ctcaccgcgg cggcgctgcg cgacaagctc tcgcaggcca
      721 tggtggtcgc gaccgtgcag gcggccaagg tgctcggcat tcattgctcg gccaagaagg
      781 ccgactcgca cgcctcactt cagtatctgg ccgcgatcgg cctcgactac gcgcagggcg
      841 ccgcgctttc gaaggccgtg cccctcgagg acatcgccac ggttgcggac ccacgcccca
      901 gcgcgatccc gcacctggga aacaacgaat aaattcagtg ccttaagggc tctgtgaagg
      961 gctctgtgcg acgaagcgag aggcgcgcgc gcccggggcg ccgacccgcg gcacgcctcg
     1021 tgccccggat gccctaagct tggccgaccc gccctgcggt ccgcctctgc gggtgcggcg
     1081 cgcgggaagg cgcgccatca aaatgccgac gctcgagcaa ttccggaaga accgcgacgt
     1141 cctgtcctgg acgctgcacg gggcgttctg ggccgcgtac ggcatcactc agtacgtagg
     1201 cgactttttc tacgcctacg gctcgtcctc gagctgggga ccactgctgc tgatcgcggc
     1261 ggtcgccggc ttcgtgctgt cggcgccgat gcgctacatc tatcggcggc tctgggggca
     1321 gcagccgcgc gccatcatcg tcggcgtgct cgtcacctgc tacatcacgg cgctggcgct
     1381 gcgcctggtc atcaacctct tctatcggga gctcatcgca ccggactggg aggtgaagac
     1441 gctcttcgag ctgttcggcg gcgcgctctc gaccgcgtat ctgctgctct gctggagcgt
     1501 gctgtatttt ggcatcaagt tctacgagtc caagcgccag caggaggagg cgatgctgaa
     1561 ggcggtggcg ctcgcgcagg aggcgcaact gaagatgctg cgctaccagc tcaaccccca
     1621 ttttctattc aatacgctga atgccatctc gacgctgatt ctcgacaacc agaaccgcaa
     1681 ggcgaaccaa gcggtggcgc ggctgtcgga gttcctgcgc tacacgctgg accaggaccc
     1741 cgtgaagaag gtgacgctgc gccaggagat cgaggctctc gacctgtatc tcggcaccga
     1801 gcgactgcgc ttcggcgagc ggctcaagct cgagtacgcg atcgaggagc cggcgctgga
     1861 cgcgctggtg ccgagcctgc tgttgcagcc gctgctcgag aactcgctga aatatgcagt
     1921 gtcggcgcgc gaacagggcg ggatggtgcg catcgagggc cgcacgcgcg aggggctgct
     1981 cgagctctcg gtgatcgacg acggcccggg gctgcccgag gggccgccgc cgggcgaccg
     2041 tcgcggcgtc gggctcgcca acacgcgcga gcggctcaag gtactctacg gcgagagctg
     2101 ccgcttcgcc gcgctcaaca cccatccggg actcagggtc gacatggcgt tgccgctcga
     2161 gacggcaccg cctgaggagc gcgcggcgca gcgggcgctc agcgcgcggc tttcgggagg
     2221 cgtccacgca tgaaggtgcg cacaatcatc gtcgacgacg aggtactgtc acggcgcggc
     2281 atcgagatcc gcacgcgcga gctgccggat ttcgagatcg tggcgcagtg cgccaacggc
     2341 cgcgaggcgc tcgccgccat ccagcagtac aagcccgacc tcgtgttcct cgacatccag
     2401 atgccggcga tgtcgggctt cgaggtgctg gcacatctgc cgcaggagtc gtttccgctc
     2461 gtggtgttcg tcaccgccta cgaccagtac gcgatccagg ccttcgaggc gcgcgccatc
     2521 gactacctcc tgaagccgat cgacgaggtg cgcttcgccg aggcagtggc gcgggtgcgc
     2581 gagcatctgc gcgcgcgcag cgccgcggca cagcgcgacc gcctcatgca gatcatcgcg
     2641 gagatcaccg gctgcgggga gctggcgctc gacgagctgc tcgagcacgg ccgtaaggct
     2701 ctcagcgggg ggcggcctga agtgctgccg atccgccagg ggcgcgagac ggtgcgcgtg
     2761 ccgatcgcga gcattcagtg ggtcgatgcg gccggcgatt acatgtgcat ccacgccgcc
     2821 ggcgagaccc acatcctgcg cggcaccatg aaggagctcg aggagctgct cgacccgaag
     2881 ctcttccagc gcgtgcaccg ctcgaccatc gtgaatctgc gcctggtacg gagcctgcgc
     2941 gcccacatga acggggagta cttcctcacg ctcgagagcg ggcacgagct gaagctctcg
     3001 cgcacctatc gcgacaagat cgagtacttc ctctccggct cgatgagctg agcgccaccg
     3061 cgggcgtcgc cgaaggcaat cgcgcgctcg tgcgagtgag aactgcgtca cgaagtgcgc
     3121 cggccgtccc agatctgtga gccacccgga tgccgctgcc ggagggcgtg gtgatcatat
     3181 gtccacgtat ggaagctgcg cgtggggacc cattgcctgg cctgccggtc gaggagtcgg
     3241 cagacggcgc ggcgcgcct
//
LOCUS       DATLKL010000484         2430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_28229, whole genome shotgun sequence.
ACCESSION   DATLKL010000484 DATLKL010000000
VERSION     DATLKL010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28229"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VM713_06460"
     CDS             complement(<1..1304)
                     /locus_tag="VM713_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HVH83941.1"
                     /translation="MTSSPDLVLVDGSSYVYRAFHALPPLSNSRGEPTGAVLGVLNML
                     QKFLKDFRPQHIAVVFDAPGRTFRDELFTEYKAQRPAMPDDLRAQVAPLLEIIQAQGL
                     PLLRVPGVEADDVIGTLARRAAAAGQQVLISTGDKDMAQLVNGSITLINTMTNTLLDR
                     EGVKLKFDVYPEQIIDYLALVGDSSDNIPGIEKVGPKTGAKLLQQYGGVDNLIAHAAE
                     VPGKVGENLRLGLATLELSRRLATIRTDLELPVALEDLKPGAPDTARLRELYTRFELR
                     ALLRSLEGGEDSAAAGAPAASAAPLPAPAVVPAPPAIERRYETLTSWEDFERWLEVLR
                     KAELFAFDTETTSLDYMRAEIVGVSFAVEPGAAAYVPLAHVYPGAPEQLDRARVLAAL
                     KPLLESAVHGKVGHNLKYDAHVLLNAGIALAGMRFDTMLESYV"
     gene            1413..>2430
                     /locus_tag="VM713_06465"
     CDS             1413..>2430
                     /locus_tag="VM713_06465"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HVH83942.1"
                     /translation="MQQLGSTIAVNRFGLGARPRELAALGGDGRDWLRAQLKAVPPAI
                     TDAQLASAAQVLGQAFALQREIQQERRSGNDGQQKLPQRLQPIYKSEATARFQQAVAT
                     ERPFLERLTQFWTNHFVVSTDKQFMAGLAGSFEREAIRPRVLGNFGDLLVAAEMHPAM
                     LLYLDNHLSVGPHSPAGLRAARRQRGNRTIGINENLGREILELHTLGVGGGYTQADVT
                     SFATVITGWSVGGGNGLFASGPPGRFVFRADQHEPGSKTVLGRRYADQGYEQGVMVLN
                     ELAQHPATARHIATKLARHFIADDPPKDSIERIARVFRDTGGDLRQVTAAVVKEDAAW
                     KTPFA"
BASE COUNT          382 a          825 c          854 g          369 t
ORIGIN      
        1 acgtacgact cgagcatcgt gtcgaagcgc atgccggcga gcgcgatccc tgcgttgagc
       61 agcacgtgcg cgtcgtactt gaggttatgg cccaccttgc cgtggaccgc gctctcgagg
      121 agcggcttga gcgcggcgag cacccgcgca cggtcgagct gctcgggcgc accgggatag
      181 acgtgggcga gcggcacata ggcggcggca cccggctcga cggcgaaaga cacgcccacg
      241 atctccgcgc gcatgtagtc gaggctcgtg gtctcggtat cgaaagcgaa cagctcggct
      301 ttgcgcagca cctcgagcca gcgctcgaag tcttcccagc ttgtcagggt ctcgtagcgg
      361 cgctcgatgg cgggcggcgc gggcaccacg gccggagccg gcagcggagc agcgctggcg
      421 gcgggggctc ccgccgccgc actgtcttcg ccgccttcga gcgagcgcag cagcgcccgc
      481 agctcgaagc gcgtgtacag ctcgcgcagc cgcgctgtat cgggcgcccc gggcttgagg
      541 tcctcgagcg ctaccggcag ctcgagatcg gtgcggatgg tcgcgaggcg ccgcgacagc
      601 tcgagcgtcg cgagcccgag gcgcaggttc tcgcccacct tgcccgggac ttccgcggcg
      661 tgggcgatca ggttatcgac gccgccgtac tgctgcagca gcttggcgcc ggtcttcgga
      721 cccacctttt cgatccccgg aatgttgtcg gagctgtcgc cgacgagggc gaggtagtcg
      781 atgatctgct cggggtagac gtcgaatttg agcttcactc cctcgcggtc gagtagcgtg
      841 ttggtcatgg tgttgatgag cgtgatcgag ccgttcacca gctgcgccat atccttgtcg
      901 ccggtggaga tgaggacctg ctgcccggca gccgccgcgc gccgcgccag cgtgccgatg
      961 acgtcgtcgg cctcgacgcc cggcacccgc agcagcggca ggccctgcgc ctgaatgatc
     1021 tcgaggagcg gcgccacctg cgcgcgcaga tcgtccggca tcgcgggccg ctgcgccttg
     1081 tactcggtga agagctcgtc gcgaaaggtc ctgccgggtg catcgaacac cacggcgata
     1141 tgctgcggac ggaagtcctt gaggaacttc tgcagcatgt tgagcactcc gagcacggcg
     1201 ccggtcggct cgccgcgcga gttcgagagc ggcggcaggg cgtggaacgc ccggtagaca
     1261 taagaggagc cgtcgacgag taccaggtcg ggggaggacg tcatgaaggg gcgctgccgg
     1321 gagcgggccg cgagtatagc ggacggcaag ccgcagcagt cccgcgcaat acgtcaacca
     1381 cttcggcgcc tggcgcgcta tagagtccac gcatgcagca gctcggctcc accattgcgg
     1441 tcaatcgctt cgggctcggc gcccgaccgc gcgagctcgc cgcgctcggc ggcgacggcc
     1501 gcgactggct gcgcgcgcag ctgaaggccg tgccgcccgc catcaccgat gcgcagctcg
     1561 cctccgcggc gcaggtgctc gggcaggcct tcgcgttgca gcgcgagatt cagcaggagc
     1621 gccgctcggg caacgacggg cagcagaagc tgccgcagcg ccttcagccg atctacaaga
     1681 gcgaggcgac ggcacgcttc cagcaggcgg tggcgaccga gcgccccttc ctcgagcggc
     1741 tgacccagtt ctggaccaat cactttgtcg tgtcgacgga caagcagttc atggcggggc
     1801 tcgccgggag ctttgagcgc gaggcgatcc gtccgcgggt gctcggcaac ttcggcgacc
     1861 tgctggtcgc cgccgagatg cacccggcga tgctgctcta tctcgacaac cacctgtcgg
     1921 tgggcccgca ttcgcccgcg gggctcagag ccgcccggcg ccagcggggc aatcgcacca
     1981 tcggcatcaa cgagaatctc ggccgcgaga tactcgagct gcataccctc ggcgtgggcg
     2041 gcggctacac gcaggccgac gtgacttcgt tcgctaccgt gatcaccggc tggtcggtgg
     2101 gcggcggcaa cgggctcttt gccagcggcc cgcccggccg cttcgtgttc cgtgcggacc
     2161 agcacgagcc tgggagcaag acggtgctcg gacggcgcta cgcggatcag ggctacgagc
     2221 agggcgtcat ggtgctgaac gaactggcac agcatcctgc gacggcgcgc cacatcgcga
     2281 ccaagctcgc gcgccacttc atcgccgacg atccgcccaa ggacagcatc gagcgcatcg
     2341 cccgcgtctt ccgcgacacc ggcggcgatc ttcgccaggt caccgccgcg gtggtgaaag
     2401 aggatgccgc ctggaagacg cctttcgcca
//
LOCUS       DATLKL010000485         1976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_118564, whole genome shotgun sequence.
ACCESSION   DATLKL010000485 DATLKL010000000
VERSION     DATLKL010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118564"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VM713_06470"
     CDS             complement(<1..288)
                     /locus_tag="VM713_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83943.1"
                     /translation="MELIQLVLNGIALGAAYALVALGFVLILNATGTVNFAQGDMVMA
                     GGFVTVALAQYLPAPGIVLLPLVFILMGALGLVFSLVAYFPLMGRPPVSVFI"
     gene            complement(323..1375)
                     /locus_tag="VM713_06475"
     CDS             complement(323..1375)
                     /locus_tag="VM713_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVH83944.1"
                     /translation="MSIPVLVPISGFLSLEGMSQRNGALLAFKHAPQGVKAEGEVSDT
                     GTSPEVAVNALEKALSEDRPVAIVASMLGVQMLAMLPVAAEHQIPLVTVSGTAKVTEL
                     GNAYVFRFFPNDAVVKLAQARYAVEVLGKTRPAILYQTTAYGQSGVAVLDPAFKQLGA
                     TVVFEEGLDVKIKDMLPILTKVREADPDVLILQLHAGSTALVIKEAAAMGLDLPIVAG
                     SAMHQPSTAALLEPAEMKGVCAETGSSPVSAESPALRKWLEEYRGAFQTDPDAYALGQ
                     YDGVLMVLKAVADGAKTAAEVRDYLASKTYKGLAMTYKSDRAGNMAHSAEIVCYDGKT
                     RVPKIMKHYENVSGVL"
     gene            complement(1625..1819)
                     /locus_tag="VM713_06480"
     CDS             complement(1625..1819)
                     /locus_tag="VM713_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83945.1"
                     /translation="MCAAPLARFVSRVDRVACANKFVQDWAQSHGQKLGDACAAPEWY
                     HQDETVAKIIAEQGKLGTGK"
BASE COUNT          338 a          668 c          656 g          314 t
ORIGIN      
        1 gatgaagacg gaaacgggcg gccgccccat cagcgggaaa taggcgacga gcgagaaaac
       61 cagaccgagg gcccccatca ggatgaacac gagcggcagc agcacaattc ccggcgccgg
      121 cagatactgg gccagcgcca cggtgacgaa gccgcccgcc atgaccatgt cgccctgggc
      181 gaagttgact gtgccggtcg cgttgaggat gagcacgaag cccagcgcca cgagcgcata
      241 ggccgcgccg agggcgatac cgttgaggac gagctggatg agctccacga ctccgagcgc
      301 gcgccgcgac gccgcgcttt ccctagagca cgccgctcac attctcgtag tgcttcatta
      361 ttttgggcac acgcgtcttg ccgtcatagc agacgatttc cgccgagtgc gccatattgc
      421 cggcacggtc cgacttgtac gtcatggcca ggcccttgta cgtcttgctc gcgagatagt
      481 cgcgcacctc ggccgccgtc ttggcgccat cggccaccgc cttcaggacc atcagcacgc
      541 cgtcgtactg gccgagcgca taggcatccg ggtcggtctg gaaggccccg cggtactcct
      601 cgagccattt cctgagcgct gggctctcgg ccgagacggg cgatgagccg gtctcggcgc
      661 aaaccccttt catctcggcg ggctcgagga gggccgcggt cgagggctgg tgcatggccg
      721 agccggcaac gatcggcaga tccaggccca tggcggccgc ctccttgatg acgagtgctg
      781 ttgagccggc gtggagctgg aggatgagca cgtcgggatc cgcctccctg accttggtca
      841 ggatcggcag catgtccttg atcttgacgt cgagcccctc ctcgaagacc accgtggcgc
      901 ccaattgctt gaatgcggga tcgaggacgg cgaccccact ctggccgtag gcggtggtct
      961 ggtagaggat ggccggccgc gtcttgccga ggacctcgac cgcgtagcgt gcctgcgcca
     1021 gcttgacgac cgcatcattg ggaaagaagc ggaagacata ggcattgccg agctcggtga
     1081 ccttggcggt gcccgacacc gtgactagcg gaatctggtg ctcggccgcg accggcagca
     1141 tggcgagcat ctggacgccg agcattgagg cgacaatggc gacgggtcgg tcctcggaga
     1201 gtgccttctc gagcgcgttg acggcgacct cgggcgaggt gcccgtgtcg gagacctcgc
     1261 cctcggcctt gacgccctgc ggcgcgtgct tgaaggcgag cagcgcgcca ttccgctggc
     1321 tcatgccctc gagcgacagg aaccccgaga tcggtaccag gacagggatg ctcacttcgc
     1381 gggcatgcgc agctcctgcc gcgaacgcca atgccgcgat cgcaccgcaa accaaggcct
     1441 ggacagctcg catcggcatg gacatcacat caactgcctc ccctggaagc gccatctcgc
     1501 ccgcccacgc taaggccggg gcggccggag tccgccgcgg gcggatgata gcccagctgc
     1561 gacgccggtc ccagggcgcc tatagctgcc gcacattgac ccagcgcgtg agaatgcggg
     1621 cgggctactt tcccgtgccg agcttaccct gctcggcgat gatcttcgcg accgtctcgt
     1681 cctggtgata ccattcgggc gcggcgcacg catcgccgag cttctggccg tgcgattgcg
     1741 cccagtcctg cacgaacttg ttggcgcagg cgaccctgtc gaccctcgaa acaaaccgcg
     1801 caagcggcgc ggcgcacaag tgcgggcgcc tctcaggccg gcagcagcgc ctcgatctcc
     1861 tcggtgatct cgcgcgcgag cgggctgacc cgcgagggcc aggcgcccgc gagcgcgccg
     1921 tccggaccga tcagatactt gtgaaagttc cagcgcggcg ccgcgtcctc gccgat
//
LOCUS       DATLKL010000486         1703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_197478, whole genome shotgun sequence.
ACCESSION   DATLKL010000486 DATLKL010000000
VERSION     DATLKL010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197478"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..637)
                     /locus_tag="VM713_06485"
     CDS             complement(140..637)
                     /locus_tag="VM713_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HVH83946.1"
                     /translation="MTTADYLIITVILISGLVGLARGLLREGVAFATWIVALILAWHF
                     SDLVEPRLGGVLAATAVKPWVARLIILLVVLLVGALVGSLANEFMRLSIFGGVDRLGG
                     FAFGTLRGLLLFGVFVMLGQLLSLDRDRWWADSHLIPVGESIANGVRFLVGEGGLPHS
                     RDIQV"
     gene            complement(731..1315)
                     /locus_tag="VM713_06490"
     CDS             complement(731..1315)
                     /locus_tag="VM713_06490"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HVH83947.1"
                     /translation="MDTLVKERLTGAFILVGLIVVLVPELLSGPIHTASRAHGPAASA
                     EGPPLRSYTITLADEPHGRSGSLQMQAASTPAAEPGGAARLPEPVPAAAPATRPAPAM
                     KASAPTTASAAAGTSAGTSGYVVQLGSFASRANAERLAAQVHGQGFAVSVSRGSAGRR
                     LYRVQVGPARDRGAAEELAAQLRAKGHGGTVVPK"
     gene            complement(1368..>1703)
                     /locus_tag="VM713_06495"
     CDS             complement(1368..>1703)
                     /locus_tag="VM713_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83948.1"
                     /translation="LRERALPGSKTGGRGRTFAVAGVLADKDAAGIAAALAPLVDHWI
                     VCALPGVRGVSAAALAERMQLPPGQFTLAASVAEGCARARARARPGDRVVVFGSFQTV
                     GPALDWLRL"
BASE COUNT          265 a          620 c          586 g          232 t
ORIGIN      
        1 cctcggccga agtcgcgatg cccgccgcgt cctggccgcg gtgctgcagc accgtgagcg
       61 catcgtacag tcgctggttg acttggctgg tcccgacgat gccgacaatg ccgcacatgg
      121 cttaggttcc ctcagctcct catacctgga tgtcgcggct gtgggggagc ccaccctcgc
      181 ccaccaggaa ccgcactccg ttcgcgatcg attcgccgac cgggatgagg tgcgaatcgg
      241 cccaccaccg atcccgatcg aggctgagaa gttgtccgag catcacgaag acgccgaaca
      301 gcagcagtcc ccgcagcgtg ccgaaggcga agccgccgag gcggtccacg ccgccgaaga
      361 tcgagagccg catgaactcg ttggcgagag agccgaccag cgccccaacg agcagcacca
      421 ccagcaggat gatgaggcgc gccacccagg gtttcaccgc ggtcgccgcc agcactcccc
      481 cgagacgcgg ctcgacgaga tcggagaaat gccaggcgag aatcagcgcg acgatccacg
      541 tggcgaacgc gacgccttcg cgcagcaggc cgcgggcgag gcccacgagg cccgagatca
      601 ggatcacggt gatgatcagg tagtcggcgg tagtcattgc cgggggcgat tcgcgcgctc
      661 gttgcgcgcg gcgcggattg tacaagagcg tcttgcgccg tgccgagcgt tgcgtcgcac
      721 gggcgcggag ctacttcggc acgaccgtgc cgccatgtcc cttggcgcgc agctgcgccg
      781 ccagctcctc ggccgcgccg cgatcgcgcg ccggtcccac ctgcacccgg taaagccgtc
      841 gcccggcgct gccgcgcgac acgctcaccg caaatccctg gccgtgtact tgtgcggcaa
      901 ggcgctccgc gttcgcgcgg ctcgcgaagc tgccgagctg cactacatag cccgaggtgc
      961 ctgcgctggt gccggccgcc gcggatgcgg tcgtcggcgc gctcgctttc atggcgggcg
     1021 cggggcgcgt tgccggtgcg gcggcgggta ccggctccgg taggcgtgcc gctccgcccg
     1081 gctccgcggc cggcgtgctc gccgcctgca tctgcagcga gccgctgcgc ccgtggggct
     1141 cgtccgcgag agtgatggtg taggagcgta atggcggtcc ctccgccgac gccgctggcc
     1201 cgtgtgcgcg tgatgccgtg tggatcggac cgctgaggag ctcgggcacc agcacgacga
     1261 tcaggcccac gaggatgaat gcgcccgtca gccgttcttt caccagagtg tccacgatga
     1321 gggcgaaccc gggaaaacca agtgctgcac cagggatccc gccggcatta tagccgaagc
     1381 cagtcgagcg ccggccccac ggtctggaag gaaccaaaca ccacgacgcg gtcccccgga
     1441 cgagcgcgcg cgcgcgctcg cgcgcagccc tccgctaccg aggcggcgag cgtgaactgc
     1501 cctggcggga gttgcatccg ctcggcgagt gccgccgcgc tcacgccgcg cacacccggc
     1561 agcgcacaga cgatccagtg atcgacgagc ggcgcgagcg cggcggcgat ccccgccgca
     1621 tccttgtcgg cgagcacgcc cgccactgcg aaggtgcgtc cccgcccccc cgtcttgctg
     1681 cccggcagtg cccgctcgcg cag
//
LOCUS       DATLKL010000487         2948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_39537, whole genome shotgun sequence.
ACCESSION   DATLKL010000487 DATLKL010000000
VERSION     DATLKL010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39537"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VM713_06500"
     CDS             <1..426
                     /locus_tag="VM713_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HVH83949.1"
                     /translation="LIRIAKLLGWRVIVSDHREAFLAARAAGAERTLCARPAEALAAV
                     GDQRLDACIVMTHTAANDRDALAALARLADPFIGLLGPLARRDELLAELDPVARGALT
                     PRLHAPVGIRLGGHGPEMLALSIGAELQRFLATATEPRI"
     gene            complement(436..1125)
                     /locus_tag="VM713_06505"
     CDS             complement(436..1125)
                     /locus_tag="VM713_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HVH83950.1"
                     /translation="MLRALRVGCLLKQSGLYSRMSIGAVLLAAGRGERLGGRPKALLE
                     LGGVPLVLRKLIALSGAGIDELVVVLGHHAASILPVIESFPVTVVHNPRPDDGQVSSQ
                     RLGLAALNPALDAVVVALADQPLIDAEDVTQVIAAFKKRPEGTAVVVPRVAGDPGNPV
                     IFSGAVREQILAGDANIGCRQWRNAHPEAVSHFDTDNRRFTVDIDTPEDLASFERCTG
                     HALRWPASASP"
     gene            complement(1110..2027)
                     /locus_tag="VM713_06510"
     CDS             complement(1110..2027)
                     /locus_tag="VM713_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HVH83951.1"
                     /translation="MTEVSNDLFAAAQRLQQQGQPYALVSVVRTEPPASARPGDKALA
                     TAQGIVHGWIGGGCAQPAVLHTVREALKDGRARLVRIAPVEAEEPRDARSLLRDVAEF
                     GMSCHSGGTLELFVDPILPPSRLVIIGDSPVAVALAGLAPRVGLQVTVIAKGADRARF
                     PDASQVIETDDTGALTAQVAPAALIVVATQGRRDLQGLRAALALNARKVWLVASTRKG
                     QVLRELLLAAQHDPAAVNAIVAPAGAPIGAQTPEEIALSVLAAVVAARRAGHSPGEQA
                     VAAEVVAEAQAEPAGDEPGHSPHQDCCGH"
     gene            complement(2024..>2948)
                     /locus_tag="VM713_06515"
     CDS             complement(2024..>2948)
                     /locus_tag="VM713_06515"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVH83952.1"
                     /translation="FDALFDGWFLPANHWQAAERHAAAHGAGGARAPAAARAEGDETD
                     AARRHELASEREAPGSADFRSLADREHLLEIEALMRRFARRLKAIRMRREARARRGRR
                     LDLAATIRRSVATGGTPLKLAWRAHRRMRPRLVLLIDVSRSMAPYSFFYLRLARALAA
                     ELTDVRSFIFHTRLTAVSEALHESDPYRAQERLHLLAQGWAGGTRIGASLEEFNREHA
                     PRCVHARTAAIILSDGYDAGDPAQLARALAELRRRARRVVWINPLAGHADYAPVSGAM
                     QAALPHLDLFAAGGDLRSLEATLPEVIEALK"
BASE COUNT          423 a         1091 c         1022 g          412 t
ORIGIN      
        1 ctgatacgca tcgccaagct gctcggctgg cgggtcatcg tctcggacca ccgcgaggcc
       61 ttcctcgccg cgcgcgcggc cggcgccgag cgcaccctgt gcgcgcgccc cgctgaggcc
      121 ctggccgccg tcggcgatca acggctcgat gcctgcatcg tgatgacgca taccgcggcg
      181 aacgaccgcg acgcgctcgc cgccctcgcg cgcctcgccg atcctttcat cgggctgctc
      241 gggccgctgg cgcggcggga tgaactgctc gcggagctcg atcccgtggc acgcggcgcg
      301 ctcacgccgc gcctgcatgc tcccgtcggc atcagactcg gcgggcacgg tccggagatg
      361 ctcgcgctgt cgatcggcgc cgagttgcag cgcttcttgg ccaccgcgac cgagccgcgg
      421 atctagatcc gatcctcagg gcgacgcgct cgccggccag cgcaacgcat gaccggtgca
      481 gcgctcgaag ctcgcgaggt cttcgggtgt atcgatatcg accgtgaagc gccggttgtc
      541 ggtatcgaag tgcgacaccg cttcagggtg ggcgttgcgc cattggcggc agccgatgtt
      601 ggcatcgccg gcgaggatct gctcgcgcac cgccccactg aagatcaccg ggttgcccgg
      661 gtcccctgcc acccgcggta caaccacggc agtaccctcg ggccgcttct tgaaagcggc
      721 gatgacctgg gtgacgtcct cggcatcgat gagcggctga tcggccagcg caaccaccac
      781 cgcatcgagc gcgggattca gcgcagccag ccccaggcgc tgtgaagaca cctggccatc
      841 gtcgggccgc gggttgtgaa cgacggtcac agggaacgac tcgatcaccg gcaggatgct
      901 cgccgcgtga tggccgagga cgaccacgag ctcgtcgatc ccggcaccag aaagcgcgat
      961 cagcttgcgc agcaccagcg gcacgccccc gagctcgagc aacgccttgg gccgcccgcc
     1021 gaggcgctcg ccccggccgg ccgcgagcag cacggcaccg atcgacatgc ggctatagag
     1081 gccgctctgc ttgagaagac agcccaccct cagtgcccgc agcaatcctg gtgggggctg
     1141 tgccccggct cgtcgcccgc cggctcggcc tgtgcctccg ccaccacctc tgccgcgacc
     1201 gcttgttccc cggggctgtg cccggcgcgg cgcgcggcga cgacggcggc cagcaccgaa
     1261 agcgcaatct cctccggcgt ctgcgcgccg atcggcgcac ccgcaggcgc gacgatcgca
     1321 ttcaccgccg ccgggtcgtg ctgggcagcg agcagaagct cgcgcagcac ctgacccttg
     1381 cgggtgctgg cgaccaacca gaccttccgt gcgttgagcg cgagcgctgc acgcagcccc
     1441 tgaagatcgc ggcggccttg cgtggcgacc acgatgagag cagctggtgc gacctgagcg
     1501 gtcaaggctc ccgtgtcgtc cgtttcgatc acctgactgg catcggggaa tcgcgccctg
     1561 tcagcgccct tggcgattac ggtgacctga agtccgaccc gcggcgcgag ccccgcaagc
     1621 gcgaccgcca ccggcgagtc gccgatgatc acgagccggc tcggcggcag gatcgggtcg
     1681 acgaagagct cgagcgtacc gccggagtga cagctcatgc cgaactcggc cacatcgcgc
     1741 agcaggctgc gcgcatcgcg tggctcctcc gcctcgaccg gagcgatgcg gaccagccgc
     1801 gcgcgaccgt ccttgagcgc ctcgcgcacg gtgtgcagca ccgccggctg cgcgcagccg
     1861 ccgccgatcc agccatgcac gatgccctgc gcggtcgcca gtgccttgtc accggggcgt
     1921 gcggaggccg gcggctcagt gcgcaccacg ctcaccagcg catagggctg gccctgctgc
     1981 tgcagccgct gcgcggccgc gaacaggtcg ttgcttactt cggtcatttg agcgcctcga
     2041 tgacttcagg aagcgtcgct tccaggctgc gaagatcccc gcccgcagcg aacaggtcaa
     2101 ggtgcgggag cgccgcctgc atggccccgc tcaccggtgc gtagtcggca tggcccgcca
     2161 gtggattgat ccagacaacg cgccgcgcgc ggcggcgcag ctccgcgagc gcccgcgcca
     2221 gctgcgccgg gtcgccggcg tcgtagccgt cgctgaggat aattgccgcg gtgcgcgcgt
     2281 gtacgcagcg cggcgcgtgc tcgcggttga actcctcgag gctcgccccg atccgcgtgc
     2341 ctcccgccca gccctgggcc agcaggtgca gccgttcctg cgcccggtag gggtcgcttt
     2401 cgtggagcgc ctccgacacg gcggtgaggc gggtatggaa gatgaagctg cgtacgtcgg
     2461 tgagctcggc cgcgagcgcc cgcgcgagac gcagataaaa gaagctgtag ggtgccatcg
     2521 agcggctgac atcgatgagc agcaccaggc gcggccgcat gcgccggtga gcacgccacg
     2581 cgagcttgag cggcgtgccg cccgtcgcga cgctgcggcg gatcgtcgcc gccaggtcga
     2641 gccgccgtcc gcggcgcgcg cgcgcctcgc gccgcatccg gatcgccttc aaccgccgcg
     2701 cgaagcggcg catcagcgcc tcgatctcga gaagatgctc gcggtcggcc agggagcgaa
     2761 agtccgccga gccgggcgcc tcgcgctcgc tcgccagctc gtgccgccgc gcggcgtcgg
     2821 tctcatcgcc ctcggcccgg gccgcggcgg gtgcccgtgc gccacccgcg ccgtgcgcgg
     2881 cggcgtggcg ctccgccgcc tgccagtgat tggccggtag gaaccagcca tcgaacagcg
     2941 catcgaag
//
LOCUS       DATLKL010000488         2974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_107182, whole genome shotgun sequence.
ACCESSION   DATLKL010000488 DATLKL010000000
VERSION     DATLKL010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107182"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..809
                     /locus_tag="VM713_06520"
     CDS             36..809
                     /locus_tag="VM713_06520"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HVH83953.1"
                     /translation="MKPCASISFLGLVWLASAWPALAQTHVDLSYPIHDRNRPQPAVI
                     DPGTASTQDAPGRPPADAVVLFDGHDLSRWQQQDGSAPKWQLGGGYFEVAPGSGYLYT
                     REAFGDCQLHVEFAEPDPPKGEDQGRGNSGVFLHGLYEVQVLDSYRSPTYPDGQAAAL
                     YGQFPPLVNASRAPGVWQSYDIIFHGPRFDATGHLTRPARMTVLHNGVLVQDDAELTG
                     PTAHHQRPPYGAQPERLPLALQDHGDPVRYRNIWIRELR"
     gene            complement(823..1386)
                     /locus_tag="VM713_06525"
     CDS             complement(823..1386)
                     /locus_tag="VM713_06525"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HVH83954.1"
                     /translation="MKRFVSLLCGTLLLFGAGGARADKYHDTIELFRNAGESAEFFHN
                     CYGYAVFPTVGKGGLVVGGAHGTGRVYAQGKYTGEASMTQLSVGFQAGGEAYSMIVFF
                     ENKTAYEEFTSGSFEFGADATAVAITAAAEASAGTTGANASASGGKKDAVTTGRYYKG
                     MSVFTIVKGGAMYQAAVGGQKFKFTPK"
     gene            complement(1452..>2974)
                     /locus_tag="VM713_06530"
     CDS             complement(1452..>2974)
                     /locus_tag="VM713_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVH83955.1"
                     /translation="RIGAVHSVVFGGFAPHELAKRIDDAHPVLVISASCGIEGQRVIA
                     YKPLLDEALELASHRVGRCIVLQRPQVTAALIAGRDLEWSAALESAPPAPCASVEATE
                     PLYILYTSGTTGKPKGVVRDHGGHAVALAWSMQAIYGMQPGDVFWAASDVGWVVGHSY
                     IVYAPLLAGCTSVLYEGKPVGTPDAGAFWRVVAQHRVNALFTAPTAFRAIKKEDPAGA
                     LIGGHDLGSLRTLFLAGERCDPDTVHWAEEKLHVPVIDHWWQTETGWPVAANCRGLTP
                     LPIKAGSASVPVPGYDVRVLDESGAAVEPGRIGNIVIRLPLPPGTLTTLWQNDTGYQD
                     SYLKRYPGYYLTGDAGFIDADGYVWIMSRIDDVINVAGHRLSTGGMEEVLAGHPDVAE
                     CAVIGVADALKGQVPLGLVVLKAGVTRPAAQLRQELVDRVRQQIGAVAAFRHVAIVAR
                     LPKTRSGKVLRSTMRSIADSQRYTTPATIDDPRALDEIAEALRALGYAAPAAAASR"
BASE COUNT          483 a         1054 c          959 g          478 t
ORIGIN      
        1 agactcgtca gcttggtgtg cctggaggat gtcccgtgaa accctgcgcg tcgatctcat
       61 tcctcggcct ggtgtggctc gcgagcgcgt ggccggcgct cgcccagaca cacgtcgacc
      121 tgagctaccc cattcacgac cgcaaccgac cacagccggc ggtcatcgat ccgggcacgg
      181 cgagcacgca ggatgcgccc ggccgtccgc ccgcggatgc cgtcgtgctg ttcgacggac
      241 acgatctttc ccgctggcag caacaagatg gcagcgcgcc gaagtggcag ctcggcggcg
      301 gttatttcga agtggctccg gggagcggct acctctatac gcgcgaggcc ttcggcgact
      361 gtcagctcca tgtcgagttc gccgagcccg atcctcccaa gggagaggat cagggccgtg
      421 gcaacagcgg agtcttcctt cacggcctct acgaggtgca ggtactcgac tcctaccgga
      481 gccccaccta cccggacggt caggccgccg ccctctacgg tcagttcccg ccgctcgtaa
      541 acgcctcacg ggctccgggt gtctggcaga gctacgacat catctttcac ggcccacgct
      601 tcgatgccac gggacatctc acccgcccgg cgcgcatgac ggtgctgcat aacggcgtgc
      661 tggtccagga cgacgcagag ctcacgggac ccaccgcaca ccaccagcga ccgccctacg
      721 gtgcgcagcc cgagagactg ccgctcgcgc tgcaggacca cggcgatccg gtccgttacc
      781 gcaacatctg gatccgcgag ctgaggtgag gagcggcgcc gcctacttcg gtgtgaactt
      841 gaacttctgg ccgccaacag ccgcctgata catggcgccg cccttcacga tggtgaacac
      901 ggacatgccc ttgtaatagc gtccggtggt gaccgcgtcc ttcttcccac cgctcgcgga
      961 cgcgttggcg ccggtcgtac cggcgctcgc ctcagccgca gccgtgatcg cgaccgcagt
     1021 ggcatccgcc ccgaactcga agctgccgct cgtgaactcc tcataggcgg tcttgttctc
     1081 gaagaagacg atcatgctgt aggcctcacc gcccgcctga aagccgacgc tgagctgcgt
     1141 catcgacgcc tcgcccgtgt actttccctg ggcatagacg cggcccgtgc cgtgggcacc
     1201 tccgaccacg aggcccccct tgccgaccgt cgggaatacc gcgtagccgt agcagttgtg
     1261 gaaaaactcg gcactctccc cggcgtttct gaagagctcg atcgtgtcat ggtatttgtc
     1321 ggcgcgcgcg ccgccggcgc cgaacaacaa taaggtaccg cagagcagac taacgaagcg
     1381 cttcatggct cttcccttct ggcgtcgtgg tggttcctgg caacattcta cgcccgcgat
     1441 gcgtcgcacc gtcagcggct ggccgccgcg gcaggggccg cgtagccgag ggctctcagc
     1501 gcctcggcaa tctcgtcgag cgcgcgcgga tcatcgatcg tcgccggcgt cgtgtaccgc
     1561 tgcgagtccg cgatcgagcg catggtcgag cgcagcacct tgcccgagcg tgtcttgggc
     1621 aggcgtgcca cgatcgcgac gtggcggaag gccgccaccg cgccgatctg ctgacgcacg
     1681 cggtcgacga gctcctggcg cagctgcgcg gccggccgcg tcactcccgc cttgagcacc
     1741 acgaggccga gcggcacttg tcccttgagt gcatccgcga cgccgatgac ggcacactcg
     1801 gcgacgtccg gatgaccggc aagcacctcc tccatcccgc cggtcgagag gcgatgcccc
     1861 gcgacgttga tgacatcgtc gatgcgcgac atgatccaca cgtagccgtc ggcgtcgatg
     1921 aagcccgcat cgccggtcag gtagtagccg gggtagcgtt tgagatagct gtcctgataa
     1981 ccggtgtcgt tctgccagag cgtcgtcagc gtgccgggcg gcagcggcag gcggatgacg
     2041 atgttgccga ttctgccggg ctcgaccgcg gcgccggact catcgagcac ccgcacgtcg
     2101 tagcccggca ccggcacgct ggccgagccc gccttgatcg gcagcggcgt gagcccgcgg
     2161 cagttggccg cgaccggcca gccggtctcc gtctgccacc agtgatcgat caccgggacg
     2221 tgcagtttct cctcggccca gtgcaccgtg tcgggatcgc agcgctcgcc ggcgagaaac
     2281 agggtgcgca gactcccgag gtcgtggcca ccgatgagcg cgccggcggg atcttccttc
     2341 ttgatggcgc ggaaagcggt cggggccgtg aacagagcgt tgacccggtg ctgggcgacc
     2401 acgcgccaga acgcgccggc gtccggcgta ccgaccggct tgccctcgta aagaacactg
     2461 gtgcagccgg cgagcagcgg cgcataaact atgtacgagt ggccgaccac ccaaccgacg
     2521 tcggatgccg cccagaacac atctcccggc tgcatgccgt agatcgcctg catggaccag
     2581 gcgagcgcga ccgcgtgccc gccgtggtcg cgcaccacgc ccttcggctt gcccgtggta
     2641 cccgaggtat agaggatgta gaggggctcg gtcgcttcca ccgaggcgca cggcgccggc
     2701 ggcgcgctct cgagcgccgc ggaccactcg aggtcacgcc cggcgatgag ggccgccgtc
     2761 acctgcggtc gctgcagcac gatgcagcgt cctacgcggt ggcttgcgag ctcgagcgcc
     2821 tcgtcgagca gcggcttgta cgcaatcacc cgctgcccct cgatgccaca ggacgccgat
     2881 atcaccaaca ccgggtgtgc atcgtcgatg cgcttcgcga gctcgtgcgg cgcgaagccg
     2941 ccgaacacca cggaatgcac cgcgccgatc cgcg
//
LOCUS       DATLKL010000489         1703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_50776, whole genome shotgun sequence.
ACCESSION   DATLKL010000489 DATLKL010000000
VERSION     DATLKL010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50776"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VM713_06535"
     CDS             <1..371
                     /locus_tag="VM713_06535"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83956.1"
                     /translation="AWYVIVYPGVGVSTREVFQSPELTRNSPLITIRAFFESGGRNDC
                     EPVVRAGSPEVAAALEWLGREGTARLTGTGSCIFLARGSAAEAERIAARVPDRWMSFV
                     ARGLDRSPVHELLQQRGRGG"
     gene            422..496
                     /locus_tag="VM713_06540"
     tRNA            422..496
                     /locus_tag="VM713_06540"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:454..456,aa:Gln,seq:ttg)
     gene            535..1473
                     /locus_tag="VM713_06545"
     CDS             535..1473
                     /locus_tag="VM713_06545"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HVH83957.1"
                     /translation="MALFAGNAIPALAHDIARSLQIPLGRAYVGRFSDGEMNVELMEN
                     VRGRDVFIVQSTCPPANDTLMELLVMVDAARRASAARITAVVPYFGYSRQDRRPRATR
                     SAITAKLVANMLSSAGVNRLLTIDLHSDQIQGFFDIPVDNVYASPVLLGDAWKQAYRN
                     VIIVSPDVGGVARARAFAKRLDDADLAIIDKRRPRPNESKVMNIIGEVEGKTCLLVDD
                     MVDTAGTLCQAAQALKDEGAARVVAYITHPVLSGAAVEKISKSALDQLVVTDTIPLSA
                     EAGKAERIRQLSVAELLAETIRRILDEGSVSSLYVD"
BASE COUNT          279 a          529 c          594 g          301 t
ORIGIN      
        1 aagcctggta tgtcatcgtc tatcccgggg tcggcgtgag cacccgcgag gtgttccaga
       61 gccctgaatt gacacgaaat tcccccctca tcacaatacg cgcctttttt gagtcgggtg
      121 ggcggaacga ctgcgagccg gtggtgcgcg cgggttcgcc cgaggtggct gcggcgctcg
      181 aatggcttgg ccgtgaaggc actgcccgcc tcaccggcac cggttcatgc atattcctcg
      241 cgcgcggcag cgcagcggag gctgagcgaa tcgcggcgcg cgtgcccgac cgctggatga
      301 gcttcgtcgc ccgcggtctg gaccgctctc cggtgcacga gctgctccag caacgcgggc
      361 ggggtggctg aagccgcagt aagatgcgcg cccgcgtcgt gttccgcccg agcggaagtg
      421 ctggggtgtc gccaagtggt aaggcagcgg gttttgatcc cgccattcgg aggttcgaat
      481 ccttccaccc cagccagttt ttagggcgca cggactatga gtaccagcga cacactggcg
      541 ctgttcgccg gcaacgccat cccggcgctg gcgcacgaca tcgcgcggag tctgcagatt
      601 ccgctcggcc gcgcctatgt gggacgtttc agcgacggtg agatgaacgt cgagctgatg
      661 gagaacgtgc gcgggcgcga cgtcttcatc gtgcagtcga cctgcccccc ggcgaacgac
      721 acgctgatgg agctgctggt gatggtggat gcggcgcggc gcgcctccgc cgcgcgcatc
      781 accgcggtgg tgccttattt cggctactcg cgccaggacc ggcggccgcg cgcgacgcgc
      841 tcggcgatca cggccaagct ggtggcgaac atgctctcga gcgccggggt caatcggctg
      901 ctgacgatcg atctgcactc ggaccagatt cagggattct tcgacatccc ggtggacaac
      961 gtgtacgcct cgccggtgct gctcggcgat gcctggaagc aggcgtaccg caacgtgatc
     1021 atcgtgtcgc cggacgtcgg cggcgtggca cgagcgcgcg ccttcgccaa gcggctcgac
     1081 gatgcggatc tcgccatcat cgacaagcgc cgcccgcggc cgaacgagtc gaaggtgatg
     1141 aacatcatcg gtgaggtcga gggcaagacc tgcttgcttg tcgacgacat ggtcgatacc
     1201 gcagggacgc tgtgccaggc ggcgcaggcg ctgaaggacg agggcgccgc gcgggtggtg
     1261 gcctacatca cccatccggt gctctcgggc gcggcggtgg agaagatctc gaagtcggcg
     1321 ctcgatcagc tggtcgtgac cgacacgatt ccgctatcgg ccgaggctgg caaggccgag
     1381 cgcatccggc agctgtcggt cgcggagctg ctggcggaga cgatccgccg gattctggac
     1441 gagggctcgg tgagctcctt gtatgtggac taggacgcag ccggcgaaag tcacgccttt
     1501 cgccggcggc aacgaagctg atgtccgccg aaatcgcttc ctgcgatttc ggcctattga
     1561 agataggtgt gccggcctgg tcgcgggccg gcatcgtttg atcaactaac ggagacttaa
     1621 acgtcatgcg tatttcattt accgtcggcg cggaagtccg cgaggcgcaa ggcaagggtg
     1681 cgagccgccg cctgcgtcac gtg
//
LOCUS       DATLKL010000490         3094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_39586, whole genome shotgun sequence.
ACCESSION   DATLKL010000490 DATLKL010000000
VERSION     DATLKL010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39586"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VM713_06550"
     CDS             complement(<1..466)
                     /locus_tag="VM713_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83958.1"
                     /translation="MGRKTPLFAQHQELGARIVDFGGWDMPVQYGSQIGEHHAVRRSA
                     GVFDVSHMCVVDLKGPRVRALLAQLLANDVAKLKNAGKALYSCMLNERGGVIDDLIVY
                     LLTESSFRAVVNAGTRDKDLDWIRRHAAACGVEVTERTDLAMLAIQGPEARAK"
     regulatory      complement(520..624)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(643..737)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            785..2023
                     /gene="ispG"
                     /locus_tag="VM713_06555"
                     /gene_synonym="gcpE"
     CDS             785..2023
                     /gene="ispG"
                     /locus_tag="VM713_06555"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HVH83959.1"
                     /translation="MSLPRRSSVQVSIGPVRVGGGAPIVVQSMTNTDTADVEGTARQV
                     QALARAGSELVRITVNTSEAAAAVAPIRAWLDRAGVTVPLIGDFHFNGHKLLREHPEC
                     AAALGKYRINPGNVGRGSKRDPQFAEMIEIACRYEKPVRIGVNWGSLDSDLLTRLMDE
                     NSARAEPLDAQAVLRNAVVLSALGSAQRAEELGLPHERIILSAKVSGVQDLIAIYRDL
                     AARCDYPLHLGLTEAGMGSKGIVASTAGMAVLLQEGIGDTIRVSLTPEPGGDRTHEVI
                     VAQEILQTMGLRAFTPLVVACPGCGRTTSTFFQELAQQIQSHIRARMPQWRREYAGVE
                     QMTVAVMGCVVNGPGESKHANLGISLPGTGERPVAPVYEDGEKTVTLKGERIAEEFQE
                     IVERYVARRYARLAPPAGAS"
     gene            2020..2361
                     /locus_tag="VM713_06560"
     CDS             2020..2361
                     /locus_tag="VM713_06560"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002019.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HVH83960.1"
                     /translation="MSESLAARRCRPCEGGTPPLTPAEAAALLAQVSSDWVLVDEARA
                     IRRNFAFRDFYRTMSFVNALAHIANIEDHHPDLEVGYNYCRVRFTPHAVKGLSENDFV
                     CAAKVDQIPLS"
     gene            complement(2381..2917)
                     /locus_tag="VM713_06565"
     CDS             complement(2381..2917)
                     /locus_tag="VM713_06565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HVH83961.1"
                     /translation="MSDPMQSPRGAAANIRAYRESDWPGVLPLLEETFRAGDSYAFSP
                     QSGAEEIHRVWVEAPARTFVACDPDGLIQGTYFIKANQPGLGAHVCNCGYVVARAAQG
                     RGIAARMCEHSQSEARGMGFRAMQFNLVVATNTRAIRLWERLGFSVVGRLPGAFHHQQ
                     LGYVDALVMFKTLVPERS"
     gene            complement(2914..>3094)
                     /locus_tag="VM713_06570"
     CDS             complement(2914..>3094)
                     /locus_tag="VM713_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83962.1"
                     /translation="MAAAIDAFDRLLAHGNGPLARLAQRGLGWVNRSQELRRFFIRRA
                     LGVSGELPELARRVS"
BASE COUNT          491 a         1076 c         1045 g          482 t
ORIGIN      
        1 ccttggcgcg cgcctccggg ccctggatcg ccagcatcgc cagatcggtg cgctcggtga
       61 cctcgacgcc gcaggcggcg gcgtgccggc ggatccagtc gaggtccttg tcgcgcgtgc
      121 cggcattgac caccgcgcgg aatgagctct cggtgagcag gtacacgatg agatcgtcga
      181 tgacgccgcc gcgctcgttg agcatgcagg agtagagcgc cttgcccgca ttcttcagct
      241 tggccacatc gttggcgagc agctgcgcga gaagtgcgcg cacccgcggc cccttgaggt
      301 cgacgacgca catgtgcgag acgtcgaaga ccccggcgga gcggcgcacg gcgtgatgct
      361 cgccgatctg ggagccgtac tgcaccggca tatcccagcc gccgaagtcg acgatgcgcg
      421 cgccaagctc ctggtgctgc gcgaacaacg gggtcttgcg tcccacttgg gtcctccagc
      481 gaacaaagaa gacggcacac gccccgcacg cgaggcgcct gccgcccttc tgtccctgtg
      541 acctgagaga tcaggcgctc tcgaagcgcc gctccccttc ggtgggtgga tagcgtcgat
      601 ccaccgctct ccagaagccc cgcggagcgg ttggaccgcc tccgccgtct gccgttcttg
      661 tgcctgagcg tttccgggcg gaatttgcgc cttcggcgtt ccggctcagc aaccggactc
      721 tctccggaca gaccgattcg ggccccggga tgataccagc gccagcgtgt aaaattcacg
      781 ccacatgagc ctcccacgcc gttcttcagt gcaggtgtcg attggaccgg ttcgcgtcgg
      841 cggcggcgcc ccgatcgtgg tccaatcgat gaccaatacc gacaccgccg acgtcgaggg
      901 caccgcacgg caggtgcagg cgctcgcgcg cgcaggctcg gagctggtgc gcatcacggt
      961 gaacaccagc gaggccgcgg ccgctgtcgc gccgatccgc gcctggctcg atcgcgccgg
     1021 ggttacggtt ccgctcatcg gcgactttca cttcaacgga cataagctgt tgcgcgagca
     1081 ccccgagtgc gccgcggcgc tcgggaagta ccgcatcaac ccgggcaacg tcggccgggg
     1141 atcgaagcgc gacccgcagt tcgcggagat gatcgagatc gcgtgccgct atgagaagcc
     1201 ggtgcgcatc ggcgtgaact gggggagtct cgactcggac ctgctgacgc gcctcatgga
     1261 cgagaactcc gcgcgcgccg aaccgctcga tgcacaggcg gtcctgcgca acgccgtggt
     1321 gctgtcggcc ttgggcagtg cccagcgtgc cgaggagctg gggcttccgc acgagcgcat
     1381 catcctctcg gccaaggtga gcggtgtgca ggacctcatc gcgatctacc gcgacctggc
     1441 ggcgcgctgc gactacccgc tgcatctcgg gctcaccgag gccggcatgg gctcgaaggg
     1501 gatcgtggcc tcgaccgccg gcatggcggt gctgctgcag gaaggcatcg gcgacacgat
     1561 ccgcgtctcg ctcacccccg agccgggcgg tgaccgcacc cacgaggtga tcgttgccca
     1621 ggagattctc cagaccatgg gactgcgggc gttcacgccg ctggtcgtgg catgtccggg
     1681 ctgtgggcgc accaccagca ccttcttcca ggagctggcc cagcagattc agtcgcacat
     1741 ccgtgcccgc atgccgcaat ggcggcgcga gtacgcgggc gtcgagcaga tgacggtggc
     1801 ggtcatgggc tgcgtcgtga acggcccggg cgagagcaag cacgccaatc tcggcatcag
     1861 cctgcccggc acgggcgagc ggcccgttgc accggtctac gaggacggcg agaagaccgt
     1921 gacgctcaag ggcgagcgca tcgccgagga attccaggaa atcgtcgagc gctacgtggc
     1981 gcgccgctac gcgcgtctgg caccgcccgc gggggcctca tgagcgagtc gctggccgcg
     2041 cgcaggtgcc ggccctgtga ggggggtacg ccgccgctca ccccggccga ggccgccgct
     2101 cttctggccc aggtctcgag cgactgggtg ctcgtcgacg aggcgcgcgc catccgccgc
     2161 aacttcgcct tccgcgactt ctaccgcacc atgagcttcg tcaacgcgct cgcgcacatc
     2221 gccaacatcg aggatcacca tccggacctc gaggtcggct acaactactg ccgcgtgagg
     2281 ttcacgccgc atgccgtgaa aggcctgtcg gagaacgact tcgtgtgcgc ggcgaaggtg
     2341 gaccagattc cgctgtcgtg acgcgaagct cagcgggcgc ttacgatctt tcgggcacga
     2401 gtgtcttgaa cattaccagg gcatcgacgt agcccagctg ttgatgatgg aacgcgccag
     2461 ggagcctccc gaccaccgag aatcccagcc gttcccacag gcggatggcc cgcgtgttgg
     2521 tggcgacgac taaattgaac tgcatcgctc tgaatcccat gccacgggcc tcggactggg
     2581 agtgctcgca catccgcgcg gcgatccccc ggccctgcgc cgcgcgcgcc accacgtagc
     2641 cgcaattgca cacgtgcgcg ccgagacccg gctgattggc cttgatgaag tacgtgccct
     2701 gaatcagccc gtccggatcg caggcgacga aggttcgcgc cggcgcctcc acccagaccc
     2761 tgtggatctc ctccgcgccg ctctgcggcg agaacgcgta gctgtcgcct gcgcggaagg
     2821 tctcctcgag caacggtagg actcccggcc agtcggactc gcggtacgcg cggatgttgg
     2881 cggcggcgcc gcgcgggctc tgcatcgggt cgctcacgac acgcgccgcg ccagctccgg
     2941 cagctcgccg ctcactccaa gcgcgcggcg gatgaagaag cgccgcagct cctggctgcg
     3001 gttcacccag ccgagcccgc gctgcgccag gcgcgcgagc ggtccgttgc cgtgggcgag
     3061 caggcggtcg aaggcgtcga tcgctgcggc catg
//
LOCUS       DATLKL010000491         2117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_5679, whole genome shotgun sequence.
ACCESSION   DATLKL010000491 DATLKL010000000
VERSION     DATLKL010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5679"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VM713_06575"
     CDS             complement(<1..190)
                     /locus_tag="VM713_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HVH83963.1"
                     /translation="MALSRKSAARSRAPAARGAHARVLRIIGGSWRGRRLRFPAAALI
                     RPTPDRVRETLFNWLGARV"
     gene            420..>2117
                     /locus_tag="VM713_06580"
     CDS             420..>2117
                     /locus_tag="VM713_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83964.1"
                     /translation="MRNLTAAGILAVALTLGGCTTHSDSVAPTNTFAANGNPGGSTPP
                     PQPGAATAFFNPATGQLPYPTDLYFAGSTDGTLNIQPPNALEPNQAAVNALDGFSTTA
                     VIREGFSTALNPATLTPATVIIVPVVTDNTKNLATVGVAGPPLTMGVDFNAALATDAG
                     VGPTILEITPLHPLNPSTCISNGTFLGASCKLGSGYLVFLTNGIADASGNAAQPDTDY
                     AAIKAALAAGGPKCPGITDVTLNGICQLTGAHLAIAAGLAQAIPALANSLNPANIVLS
                     FSFTTESTTDTLELLYQKTAATAKVVPPTIVVQPAILPTAGGPLQLTTKTFGGIGNAN
                     IYVGTLVVPYYLSKAAPDTGSWTAPPLPAPLPPSTFVTRYNPLPVATANLQIPILLSV
                     PISIPGPWPVLIFQHGITRSREDMIGVADAFAQFGFVVAAIDLPLHGVTNTTTDPLYA
                     SAANPVYAGLGLPASQMSIERTFDLALTAPPALDPSGSHFINLTSALTSRDNLREGPA
                     DLMTFELALPNLVVPASAGITASKLGPGPLHYLGHSLGAIVGGVFAAVVPSTDVSTVT
                     "
BASE COUNT          350 a          793 c          641 g          333 t
ORIGIN      
        1 caacgcgcgc gccgagccag ttgaacagcg tctcacgcac ccggtcgggg gtcggccgga
       61 tgagcgccgc cgccgggaac cgcagcctcc ttccgcgcca gctgccgccg atgatgcgta
      121 gcacgcgcgc gtgggcgccg cgcgcagcgg gagcgcggct gcgggcggcg gacttgcggc
      181 tcaacgccac ggcgtttgtc tcaagacggc gcgcaccttg ccacgattct cgccctaagc
      241 gtactctaga ttgcctcgtc cagaagcgct caaacagcac cggcgggagg ggtcgcgacc
      301 ggtggggcgg cggcctgcaa gaaggcagtt cgcggctata cgaagaatac cgaccgtggc
      361 gtgatccacc cctcgcggcg cagcgcccag gcggtctgag agttaggagt tctagggata
      421 tgcgaaacct cactgcggcc gggattctcg cggttgcctt gacactgggc ggctgcacga
      481 cgcatagcga ctccgtcgct ccgaccaaca ccttcgccgc caacggcaat cccggcggat
      541 cgacgccgcc gccgcagccc ggagccgcca cggcgttctt caatcccgcg accgggcagc
      601 tgccctaccc gaccgacctg tacttcgcag gctcgaccga cggcacgctc aacattcagc
      661 cgccgaatgc gctcgagccc aaccaggccg cggtgaacgc gctcgacggt ttctcgacca
      721 cagcggtgat ccgcgagggc ttcagcaccg ctttgaaccc ggccacgctc acgcccgcca
      781 cggtcatcat cgtgccggtg gtcaccgaca acacgaagaa cctggctacc gtcggcgtcg
      841 ccgggccccc gctcacgatg ggtgtcgatt tcaacgccgc gctcgcgacc gacgcgggtg
      901 taggtcctac gatcctggag atcacgccgc ttcatccgct caatcccagc acctgcatca
      961 gtaacggtac ttttcttggt gcgagctgca agctcggaag cggctatctg gtgttcctta
     1021 cgaacggcat cgccgatgcc tccggcaatg ctgcccagcc ggacaccgat tacgcggcca
     1081 tcaaggcggc gctggccgcg ggtggaccca agtgcccggg catcaccgac gtgacgctga
     1141 acggcatctg ccagctgacc ggcgcgcacc tcgcgatcgc ggcggggctc gcccaggcga
     1201 taccggcgct cgccaacagt ttgaacccgg ccaacatcgt gctgtccttc agcttcacga
     1261 cagagtcgac caccgacacg ctcgagctcc tgtaccagaa gaccgcggcg accgccaaag
     1321 ttgtgccgcc gacgattgtc gtacagccgg cgattctgcc gaccgccggt ggcccgctgc
     1381 agctcaccac caagaccttc ggcggcatcg gcaacgccaa catctacgtg ggtacgctcg
     1441 tagtccccta ttacctgagc aaggccgccc ccgacaccgg ctcctggacc gcgccgccgt
     1501 tgccggcacc gctgccgccc agcaccttcg tcacgcgcta caacccgctg ccggtggcga
     1561 ctgcgaatct ccagatcccg atcctgctga gcgtgcccat cagcatcccc ggcccctggc
     1621 cggtgctcat cttccagcac ggcatcaccc gcagccgcga ggacatgatc ggcgtcgccg
     1681 acgccttcgc gcaattcggc ttcgtggtag ccgcgatcga cctgccgctg cacggggtga
     1741 ccaacacgac gaccgacccg ctgtatgcga gcgcggctaa tcccgtctat gccggcctcg
     1801 gtttgccggc cagccagatg tcgatcgagc gcaccttcga tctcgcgctc actgcgcccc
     1861 cggcgctcga cccctccggc tcgcacttca tcaacctgac gagcgcgctc acctcgcgcg
     1921 acaacctgcg cgaggggccg gcggatctca tgaccttcga gctggcgcta cccaatctcg
     1981 tcgtaccggc gagcgccggc atcaccgcca gtaagctcgg cccggggccg ctgcattatc
     2041 tcggtcattc tctcggcgcc atcgtcggcg gcgtgttcgc ggcggtcgtc ccgtcgaccg
     2101 acgtgagcac ggtgacg
//
LOCUS       DATLKL010000492         2136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_152567, whole genome shotgun sequence.
ACCESSION   DATLKL010000492 DATLKL010000000
VERSION     DATLKL010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152567"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VM713_06585"
     CDS             complement(<1..380)
                     /locus_tag="VM713_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83965.1"
                     /translation="MNHRPLKSALISLALAHLLLTPLVAVSQTAWTADPEVQRRLAGG
                     EVVVQSDTAADPDHPRGRVRAAVLIRAPPGAVWAVMTDCRQTLLFVPGLRRCRRVDGA
                     PDGRWEDIEQEIRYAWFLPAVRYVL"
     gene            complement(465..1391)
                     /locus_tag="VM713_06590"
     CDS             complement(465..1391)
                     /locus_tag="VM713_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HVH83966.1"
                     /translation="MRRIRIIDSHTGGEPTRVVVEGGPDLGKGPLSRRLELLRDGYDQ
                     FRSAVVNEPRGNDAIVGAWLVPPTDSSYAAGVIFFNNADYLGMCGHGTIGLAVTLAYL
                     GRIAPGVHRIETPVGVVTATLHADLSVSVTNVPSRREARALEVEVPGIGAVTGDVAWG
                     GNWFFLIESHGQELELANVGALTDYCARVRAAVNAQGHPEVDHVELFGPARLPGANAR
                     NFVLCPGNAYDRSPCGTGTSAKLACLAADGKLAEHQEWVQESIIGSTFRATYRRSGPG
                     AVVPTIRGTAYVNADATLLLDERDPFCFGIRR"
     gene            complement(1388..>2136)
                     /locus_tag="VM713_06595"
     CDS             complement(1388..>2136)
                     /locus_tag="VM713_06595"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVH83967.1"
                     /translation="EGNSLSAAEKRTVLRGAVEVTEGRVPIIVGVSEFTTATAATFVR
                     DAERIGASGFMVLPGMVYVPTAAELEHHFRTVAAATGLPIMLYNNPPSYRVNIELPTL
                     ARLAEVPNIVAIKESAPDPRRFTDLFNAFGERFLLFAGLDDVVFEAVTLGACGWVSGL
                     TNVFPAESLALYAALAASDLERARRIYRWFMPLLHLDAEHDLVQSIKLAEQLLGRGSE
                     RVRPPRLPLAGERRAQVTALIEKARATRPV"
BASE COUNT          331 a          738 c          772 g          295 t
ORIGIN      
        1 aggacgtagc ggaccgccgg aagaaaccag gcgtagcgga tctcctgctc gatgtcctcc
       61 cagcgtccgt ccggggcacc atcgacccgg cggcagcgcc tgagtcccgg aacgaacagg
      121 agggtctgcc ggcagtcggt catcacggcc cacacggccc ccgggggcgc ccggatgagc
      181 accgccgcgc gcacccgtcc ccgcggatgg tccggatcgg ccgccgtgtc ggactgcacc
      241 accacttctc cgcccgcgag gcgccgctgc acctccggat ctgcggtcca ggcggtctgg
      301 gagacggcga cgagcggcgt gagcagcagg tgcgccagcg cgaggctgat cagggccgat
      361 ttgagggggc ggtgattcat cgcgcacgca gcgccgctga ggcgccaggg tgcgccaatc
      421 gtgagtgcgc cgggcagcgc gccgcgttca ttccgcgggc acgatcaccg cctgattccg
      481 aagcagaagg ggtcgcgttc gtcgagcagc agggtcgcat ccgcgttcac gtaggcagtg
      541 ccgcggatgg tcgggacgac ggcgccgggg ccgctgcgcc ggtacgtggc ccggaacgtg
      601 ctgccgatga tgctctcctg cacccactcc tggtgttctg cgagcttgcc gtctgcggcg
      661 aggcaggcga gcttggcgct ggtgccggtc ccgcacggcg agcggtcgta cgcgttgccc
      721 gggcagagca cgaagttgcg cgcattggcg cccggcagcc gtgccggtcc gaagagctca
      781 acgtggtcga cctcggggtg gccctgggca ttgacggcgg cccgcacccg tgcgcaatag
      841 tcggtgaggg cgccaacatt ggcgagctcg agctcctggc cgtgcgactc gatgaggaag
      901 aaccagtttc cgccccaggc gacatcgccc gtcacggcgc cgatcccggg gacttcgacc
      961 tccagcgcgc gcgcctcgcg gcggctcggg acgttcgtga ccgacaccga gaggtccgcg
     1021 tgcagcgtcg cggtcaccac gcctacgggt gtctcgatgc gatgcacacc gggtgcgatg
     1081 cggccgaggt aggcgagcgt gaccgcgagc ccgatcgtgc cgtggccgca catgccgagg
     1141 tagtccgcat tgttgaagaa gatgacgccg gcggcgtagg acgaatccgt ggggggcacg
     1201 agccaggccc cgacgatcgc atcgttcccg cgcggctcgt tgaccaccgc cgagcggaac
     1261 tggtcgtagc catcacgcag cagctcgagc cgccggctca aagggccctt ccccagatcc
     1321 ggacccccct cgacgaccac gcgcgtcggc tcaccgcccg tgtgcgagtc gatgatgcgg
     1381 atgcgcctca taccgggcgc gtggcgcgcg ccttctcgat cagtgcggtg acctgggcgc
     1441 ggcgctcccc cgcgagcggc agccgcggcg ggcgcacccg ctccgagccg cgcccgagga
     1501 gctgctcggc gagcttgatc gactgcacca ggtcgtgctc ggcatcgagg tgcaagagcg
     1561 gcatgaacca gcgatagatg cgccgcgccc gctcgagatc ggaggcagcg agcgccgcgt
     1621 acagcgcgag cgattcggcg gggaacacgt tggtgagccc ggagacccag ccgcacgccc
     1681 cgagcgtcac ggcctcgaac accacgtcgt cgaggcccgc gaacagcagg aagcgctcgc
     1741 cgaaagcatt gaagagatcg gtgaagcggc gcgggtcggg cgccgactcc ttgatggcga
     1801 cgatgttcgg cacctcggcg aggcgcgcga gcgtcggcag ctcgatgttg acgcgatagg
     1861 agggcggatt gttgtagagc atgatcggca gccctgtggc cgccgccacc gtgcggaagt
     1921 gatgctcgag ctcggccgcc gtcggcacgt acaccatgcc cgggagcacc atgaagccgc
     1981 tggcgccgat gcgctcggcg tcgcgcacga aggtggccgc cgtggcggtg gtgaactccg
     2041 acaccccgac gatgatgggc acacgccctt cggtcacttc gaccgcgccg cgcagcaccg
     2101 tgcgcttctc cgcggcactg agggagttac cctccc
//
LOCUS       DATLKL010000493         1566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_163588, whole genome shotgun sequence.
ACCESSION   DATLKL010000493 DATLKL010000000
VERSION     DATLKL010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163588"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VM713_06600"
     CDS             <1..200
                     /locus_tag="VM713_06600"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HVH83968.1"
                     /translation="EETILKVHEALEVLAQADPRLAQVAQMRYFGGYSEQEIAETLEV
                     SERTVQRDWEKARMILAAAVS"
     gene            467..>1566
                     /locus_tag="VM713_06605"
     CDS             467..>1566
                     /locus_tag="VM713_06605"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVH83969.1"
                     /translation="MIRALGAGGMAEVWLARRADGAMKRTVALKMPTLILVRSELEQR
                     FARERDILASLEHPHIARLYDAGIDPDRQPYLAMEYVEGQPLTDWCDAHRLPITARLE
                     LCLQVLEAMQYAHDRHVVHRDLKPSNILVTDSGQVRLLDFGVAKLLEEETEQAQLTSV
                     YGRALTPDYASPELLRGERLDARCDIYSFGVMLYELLIGIRPYQLKSAGSVGLLEQAI
                     ASVEVEKPSVRAEPQAAALRATTPEGLARQLRGDLDAIAVKTLAKAPAERYPSAADLA
                     KDLRSYLERRPVAARPARFVYRLRKFVGRNRTVVGVSTAAAAAVLAAFAYVVYREVTH
                     TVGSSSVTVSAAAAPEKSIAVLPFVNMSPDKE"
BASE COUNT          267 a          492 c          545 g          262 t
ORIGIN      
        1 gcgaggagac catcttgaag gtgcatgagg cgctggaggt tctggcgcaa gcggatcctc
       61 gtttggcaca agtcgcgcag atgcgctact tcggcggtta cagcgagcag gagattgctg
      121 aaacgctcga agtcagcgaa cgcaccgtgc agcgcgactg ggagaaggct cgaatgatcc
      181 tcgccgcggc ggtcagctga ccatggagct atcggcctct cagatggcgg tgatgagccg
      241 cctgctcgat gaagcgctgc cgctggatac cgccgcgcgg cgcgcctggc tcgagaagct
      301 gtcacccgag tattcggacg ttgcgccggc gctctgggaa gcgttgctcc cgtcacagtc
      361 tcgggccgcg gggccccttg gcacgttgcc gaagctgcct gccggcgggg cgagtgctgg
      421 gcccgcgcgc tcccggcaag ccggcgcgcg cgtgggaccc tacgagttga ttcgcgcgct
      481 cggcgccggc gggatggcag aggtgtggct cgcgaggcgg gcagacggcg cgatgaagcg
      541 cacggtggcc ttgaagatgc ctacgctcat cctagtgcgc agcgagctcg aacagcgctt
      601 cgcccgcgag cgcgacatcc tggcgagcct cgagcatccg catattgccc gcctctacga
      661 cgccggcatt gaccccgaca ggcagccata tctcgcgatg gagtacgtgg agggacaacc
      721 cctgaccgac tggtgcgatg cgcatcgttt gccgatcacc gctcgcctcg aattatgcct
      781 gcaggtgctc gaggcgatgc agtatgcgca tgacagacac gtcgtccacc gcgacctcaa
      841 accctccaac atcctggtga ccgattccgg ccaggtgcgc ctcctcgact ttggcgttgc
      901 gaagcttctg gaagaagaga cagagcaggc gcagctcacg agcgtctacg gtcgggcgct
      961 gactccagac tacgccagcc ccgaactgct gcgcggcgag cggctcgacg cccggtgcga
     1021 catctattcg ttcggggtca tgctgtacga gctgctcatt gggatccgcc cctatcaact
     1081 gaagagcgcc gggtcggtgg gattgctcga gcaggcgatt gcgagcgtcg aggtggaaaa
     1141 acccagcgtg cgggctgagc cgcaagcggc ggcgctgcgc gcgaccacgc ccgaagggtt
     1201 ggcgcggcaa ttgcgcggcg atctggatgc gatcgcggtc aagacacttg cgaaggcgcc
     1261 ggccgagcgc tatccgagcg cggccgacct cgcgaaggac ttgcgaagct atctcgagcg
     1321 caggcccgtc gcggcgcggc ccgcccgatt cgtctatcgg ctgcgcaagt tcgtggggcg
     1381 caataggacc gtcgttgggg tcagcacggc ggccgcggcg gccgtgctcg ctgcgttcgc
     1441 ctacgttgta tatcgagagg taactcacac ggtcggatcg agctccgtca ccgtttcggc
     1501 ggcggccgcg ccggagaagt cgatcgcggt gctgccgttt gtgaatatga gcccggataa
     1561 ggagca
//
LOCUS       DATLKL010000494         4039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_62178, whole genome shotgun sequence.
ACCESSION   DATLKL010000494 DATLKL010000000
VERSION     DATLKL010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62178"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..225
                     /locus_tag="VM713_06610"
                     /pseudo
     CDS             112..225
                     /locus_tag="VM713_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            268..459
                     /locus_tag="VM713_06615"
     CDS             268..459
                     /locus_tag="VM713_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83970.1"
                     /translation="MPFGKFAEMLGGYGEEVHDPAQIAPALQRARESVRKLKKSAVIN
                     IWVDPREYAPGTKNQTMYK"
     gene            483..1730
                     /gene="frc"
                     /locus_tag="VM713_06620"
     CDS             483..1730
                     /gene="frc"
                     /locus_tag="VM713_06620"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HVH83971.1"
                     /translation="MARALDGVRILDFTHVQSGPTCTQLLAWFGADVIKVERAGAGDI
                     TREQLRDPPDVDSLYFTMLNSNKRSITLDTKNARGKAVLERLVKHCDVLVENFAPGAL
                     DRMGLTWERIQQLNPRMIYASVKGFGPGPYEDCKVYENVAQCAGGAASTTGFDDGPPL
                     VTGAQIGDSGTGLHLALGIVAALYQRNTSGRGQRVLAAMQDGVLNLCRVKLRDQQRLA
                     RTHVMKEYPQYPDGQFGSAVPRAGNASGGGQPGWIIKCKGWETDPNAYLYFITQAPVW
                     PEICKVIGKPEWITDPDYATPAARLPRLKHVFDTIEQWTRTKTKFEAMDELNRHDIPC
                     GPILSMEELAAEPSLRATGTVVEVDHPTRGKYLTVGNPIKMSDSPTEVKRSPLLGEHT
                     AEVLGELGIGAAEIAALRAEKVI"
     gene            1804..>4039
                     /locus_tag="VM713_06625"
     CDS             1804..>4039
                     /locus_tag="VM713_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HVH83972.1"
                     /translation="MAHDKNAVRKILDQAKAAARGSLTAPEAKGICEAYGIAVPKEAL
                     AATAAEAARLAASIGFPVVMKIVSPQILHKTEAGGVVVGVKTAAEAEQAFTTIVANAR
                     RYDAKASIDGVQVQQMLTGGQEVIIGAVTDPAFGKLVAFGLGGVLVEVLKDITFRLAP
                     ATREDALSMLDGIAAAQILKGVRGAPPVDREALAAMIVNVSQLVADFPEIAELDLNPV
                     FATPVSATAADVRVVLDFKPQPARYRPDQATIVRQMNRIMKPDAVAVIGASAENGKIG
                     NSVMKNLINGGYQGAIYPIHPSATEILGRKAYKSVEDVPGVIDVAVFCIPAKFVAQAL
                     TEVGEKKIPGAVLIPSGFAEVGNVKGQEEIVAVARKYDVRLMGPNIYGFYYTPKNLCA
                     TFCTAYDVKGGAALSSQSGGIGMAIIGFSRSAKMGVSAIVGLGNKSDIDEDDLLDFFE
                     QDDNTQIICQHLEDLKDGRAFAEVAKRVAKKKPVVVLKAGRTSLGARAASSHTGALAG
                     NDKIYDDVFRQAGVIRARTLRDLLDFARAIPVLPTPKGNNVVIITGAGGSGVLLSDAC
                     VDNQLQLMAMPPDLDAAFRKFIPPFGAAGNPIDITGGEPPTTYQNTVRLGLADERIHA
                     LILGYWHTIITPPMVFARLMVEVVNEMKAKGIVKPVVASLAGDVEVEEASEYLYQHGI
                     PAYAYSTEIPVAVLGAKYRWARGAVGMYGTSYSAFNSLQVAFLYHPPALKAIFALCGT
                     DDRYT"
BASE COUNT          735 a         1379 c         1316 g          609 t
ORIGIN      
        1 tggatggatc cgggggccct cggctcactc ggcgtcggca ccggcttagc gatcgccgcc
       61 gggcttgccc atccagacaa ggaagtgttg tgctactaag gcgacggctc cttcagcatg
      121 accgccttcg acatggagac cgccaaccgt ttcggcgtgc cttatgtcgc cgtggtcggc
      181 aacaactccg cgatgaacca gatccgttac ggacagatcg cctagtacgg cgaggagcgc
      241 ggcaacgtcg gtaatctcct cggcgacgtg cccttcggca agttcgccga gatgctcggc
      301 ggctacggcg aggaggtcca tgatccggca cagatcgccc cggcgctgca gcgcgcgcgc
      361 gagtccgtgc gcaagctgaa gaaatccgcc gtgatcaaca tctgggtcga cccgcgtgag
      421 tacgcgcctg ggaccaagaa ccagacgatg tacaagtagc ccaaggacag ggagacgctc
      481 agatggccag ggccctcgac ggtgtgcgca tcctcgactt cacccacgtg cagtcggggc
      541 cgacctgcac gcagttgctc gcctggttcg gcgccgacgt gatcaaggtc gagcgcgccg
      601 gcgcgggcga catcacccgc gagcagctgc gcgacccccc ggatgtcgac agcctctact
      661 tcacgatgct gaacagcaac aagcgctcga tcacgctcga caccaagaac gcgcgcggta
      721 aggcggtgct cgagcggctg gtgaagcact gcgacgtgct ggtcgagaac ttcgcccccg
      781 gggcgctcga tcgcatgggc ctcacctggg agcgcatcca gcagctcaat ccgcgcatga
      841 tctacgcctc ggtcaagggc ttcggcccag ggccctacga agattgcaag gtctacgaga
      901 acgtcgcgca atgtgcgggc ggcgccgcct ccactaccgg cttcgacgac gggccgccgc
      961 tcgtaacggg cgcgcagatc ggcgacagcg gcacgggact gcacctggcg ctcggcatcg
     1021 tggcggcgct ctatcagcgc aacacgagcg gccgggggca gcgggtgctc gccgccatgc
     1081 aggacggagt gctgaacctc tgccgcgtga agcttcgtga ccagcaacgc ctcgcgcgca
     1141 cccacgtcat gaaggagtat ccgcagtacc cggacggcca gttcggcagc gcagtgccgc
     1201 gtgccggcaa cgcctccggc ggcggccagc ccggctggat catcaagtgc aagggctggg
     1261 agaccgatcc caacgcctat ctctacttca ttacccaggc accggtgtgg ccggagatct
     1321 gcaaggtgat cggcaagccc gagtggatca ctgatcccga ctacgccacg cccgctgcgc
     1381 gcctgccgcg tctcaagcac gtgttcgata cgatcgagca gtggaccaga actaagacca
     1441 agttcgaggc catggacgag ctcaacaggc acgacatccc gtgcggaccg attctctcga
     1501 tggaggagct ggcggcggag ccctcgctgc gcgccaccgg cacggtcgtc gaagtggacc
     1561 atccgacccg cggcaagtac ctcacggtgg gcaaccccat caagatgtcg gacagcccca
     1621 ccgaggtgaa gcgctcgccg ttgctcggcg agcacaccgc agaggtgctg ggggagctcg
     1681 gcatcggcgc ggcggagatc gccgccttgc gggccgaaaa ggtgatctga aactctgaag
     1741 gctggcagga caagcgcacc cgcttgggct cggggcgtac ggaaagggga tccgggagag
     1801 accatggcgc acgataagaa tgcggttcgg aagattcttg accaggccaa ggcggccgcg
     1861 cgcggctcgc tgaccgcgcc cgaggcgaag ggcatctgcg aggcgtacgg catcgcggtg
     1921 ccgaaggagg cgctcgccgc caccgcagcc gaggccgccc ggctcgccgc ctcgatcggc
     1981 ttcccggtcg tgatgaaaat cgtctcgccg cagatcctgc acaagaccga ggccggcggg
     2041 gtcgtggtgg gcgtcaagac cgccgccgag gccgagcagg ccttcacgac catcgtcgcc
     2101 aacgcccgcc gctacgacgc caaggcgagc atcgacggcg tgcaggtgca gcagatgctc
     2161 accggcggcc aggaagtcat catcggcgcg gtcaccgacc cggccttcgg caagctggtc
     2221 gccttcggcc tcggcggtgt gctggtcgag gtgttgaagg acatcacctt ccgtctggcg
     2281 ccggccaccc gcgaggatgc cctctcgatg ctcgacggca tcgccgcggc gcagatcctc
     2341 aagggcgtgc gtggcgcgcc gcccgtcgat cgggaagcgc tcgccgccat gatcgtcaac
     2401 gtctcgcagc tggtcgccga cttccccgag atcgccgagc tcgacctcaa cccggtgttc
     2461 gcgacacccg tgagcgccac cgccgccgac gtgcgtgtgg tgctcgactt caagccgcag
     2521 ccggcccgct accgccccga ccaggcgacg atcgtccggc agatgaaccg gatcatgaag
     2581 ccggatgcgg tcgccgtcat cggcgcatcg gccgagaacg gcaagatcgg caactcggtg
     2641 atgaagaacc tcatcaacgg cggctaccag ggcgcgatct atcccatcca cccgagcgcc
     2701 acggagatcc tcggccgcaa agcctacaag agcgtcgagg acgtgccggg ggtgatcgac
     2761 gtcgcggtgt tctgcatccc cgcgaaattc gtcgcccagg cactcaccga ggtcggcgag
     2821 aagaagatcc cgggcgcggt gctgatccct tcgggcttcg ccgaggtcgg caacgtcaag
     2881 ggccaggagg agatcgtggc ggtcgcgcgc aagtacgacg tgcgcctcat gggtccgaac
     2941 atctacggct tctactacac gccgaagaat ctgtgcgcga cgttctgcac tgcctacgac
     3001 gtcaaggggg gcgcggcgct ctcctcgcag tcgggcggca tcggcatggc gatcatcgga
     3061 ttctcccgct cggcgaagat gggcgtgtcc gcgatcgtcg ggctcggcaa caagtccgac
     3121 atcgacgagg acgatctgct cgacttcttc gagcaggacg acaacaccca gatcatctgc
     3181 cagcacctcg aggacctgaa ggacggccgc gccttcgccg aggttgccaa aagggtcgcg
     3241 aagaagaagc cggtggtcgt gctcaaggcg ggccgcacca gcctcggggc ccgcgcggcg
     3301 agctcgcaca ccggggccct cgccggcaac gacaagatct atgacgacgt gttccgccag
     3361 gcgggcgtca tccgcgcgcg cacgctccgt gacctgctgg atttcgcgcg cgccatcccc
     3421 gtgctgccca cacccaaggg caacaacgtc gtgatcatca ccggcgccgg cggctcgggc
     3481 gtgctgctct cggatgcctg cgtcgacaac cagctgcagc tgatggccat gccaccggat
     3541 ctggatgctg ccttccgcaa gttcattccg ccgttcggcg ccgcgggcaa ccccatcgac
     3601 ataaccggcg gcgagccgcc gaccacctac cagaacaccg tgcggctcgg gctcgcggac
     3661 gagcgcatcc acgcgctcat cctcggctac tggcacacga tcatcacgcc gcccatggtc
     3721 ttcgcacgtc tcatggtcga agtcgtcaac gaaatgaaag ccaagggcat cgttaagccc
     3781 gtagtcgcct ctcttgccgg cgatgtcgag gtcgaggaag cctcggaata tctctaccag
     3841 cacggcattc ccgcctacgc gtactcgacc gagatcccgg tcgcggtgct cggcgcgaag
     3901 tatcgctggg cgcgcggcgc ggtcggcatg tacggcacgt cctactcggc gttcaactcg
     3961 ctgcaagtcg ctttcctgta ccacccgccg gcgctcaagg cgatcttcgc cctgtgcggc
     4021 acggatgacc ggtacacgg
//
LOCUS       DATLKL010000495         3253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_50814, whole genome shotgun sequence.
ACCESSION   DATLKL010000495 DATLKL010000000
VERSION     DATLKL010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50814"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2280)
                     /locus_tag="VM713_06630"
     CDS             complement(<1..2280)
                     /locus_tag="VM713_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH83973.1"
                     /translation="MKDSARAVVIGGGVVGASVLYHLAKIGWSDVLLIERSELTSGST
                     WHAAGGMHTFNGEANISRLQKYTIDLYREIERLSGQSCGLHPNGGLMLAANQGELDSL
                     RLICSRARYLGMETEMISLEQALEYNPLIDPSHFIGALWRADGGHCDPSGTTNAYAKA
                     ARQLGASVERFTRVLSLSSRRDGTWDVVTDKGAVHAEHVVNCAGLWAREVGHMVGIEL
                     PVLAMEHHYLITEDLPELKGRAREIVNTTDYAGEIYVRQERGGALIGTYEPHGIVWSP
                     LKTPDDFSMQLLPEDFERLAPYFEVGFRHFPALGSVGIRKAVNGPFTFAPDGNPLVGP
                     VRGLRNYWVACAVMAGFSQGGGIGLVLSRWMAENDPGQDIISMDVARFGAFATPKYTS
                     IKVPENYSRRFRLAYPNEELPAARPVRRSPIYEQLKAARACMGANYGLENALWFAPPG
                     MEPVETPTYRRSEAFPIVRAECQAVRSAVGLYETTNYGKYEVAGHGARAWLDRVFASR
                     IPKPGRLALAPMLNPAGRIVGDLTIACLAEDRFLIFGSGFAEEFHLRWFWQSDPPADV
                     FVRSLASTLNGVSIAGPRSRELVQRLVRLDLSAGAFKLFQVRDTAVGFAPAILSRAGF
                     TGELGYEIWTTPDYFATLYDDLMDAGGAVGLRLFGGRALSALRLEKGYGSFQKDFRPD
                     YTAAESGLDRFVDFTKPDFVGRTATLAERAAGPKRRFVVMEVAALDAEVIGYESIMKD
                     GAAVGYVTSGAFGHLVGKSL"
     gene            complement(2277..2843)
                     /locus_tag="VM713_06635"
     CDS             complement(2277..2843)
                     /locus_tag="VM713_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83974.1"
                     /translation="MSAPEGIVLADCRADIVEVATFRDRAADLQRLTAERGLRLPAPG
                     SIEARSGRLALNVRPGRWLLLAPPAPPDESAGHWREMCGGYAAVVDLSAALAAFFLTG
                     SQVGGMLARACRLDLDAGAFPAGSAAATIMVQVPAILARLTPGMLLLTPATTARHLRE
                     WLVASSKPFGLALAADATVSALWGDGST"
     gene            complement(2830..>3253)
                     /locus_tag="VM713_06640"
     CDS             complement(2830..>3253)
                     /locus_tag="VM713_06640"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HVH83975.1"
                     /translation="EKGHVTTAELNGNTSADDLGFQRMLKKSGDFVGRSLAARPGLTA
                     ADRLQLVGVRPVDRGRRLRNGMHLLAPEGRSASLGYITSSTPSVELEGWVGLALLAAG
                     RQRIGTRLVGTSPIHAETTELEIVSPHMLDPENTRVRA"
BASE COUNT          534 a         1104 c         1142 g          473 t
ORIGIN      
        1 caggctcttg ccgaccaagt ggccgaacgc gcccgaggtc acgtagccga ccgcagcgcc
       61 gtccttcatg atcgactcgt agccgatcac ctcggcatcg agcgcggcca cctccatgac
      121 gacgaagcgc cgcttcggtc cggccgcccg ctcggcgagc gtcgccgtgc ggcccacgaa
      181 gtccggcttg gtgaaatcga cgaaacggtc gagcccgctc tcggcggcgg tgtaatcggg
      241 ccggaagtcc ttctgaaagg agccgtagcc tttctcgagg cggagcgcag agagcgcccg
      301 ccccccgaag agcctcaggc caacggcgcc tccggcgtcc atgagatcgt cgtagagcgt
      361 cgcgaagtaa tccggggtgg tccagatctc atagccgagc tcgccggtga agccggcgcg
      421 gctgaggatc gcgggcgcga agccgaccgc cgtgtcgcgc acctgaaata gcttgaaagc
      481 gccggccgag agatcgaggc ggaccaggcg ctgcaccagc tcgcgcgagc gtggtccggc
      541 gatcgacacc ccgttcaggg tcgaggccag cgagcgcacg aagacatccg ccggcgggtc
      601 ggactgccag aaccaccgca gatgaaattc ttcggcgaag ccggagccga agatcaggaa
      661 gcgatcctcg gcgaggcagg cgatggtgag atcgccgacg atgcggccgg cgggattcaa
      721 catgggcgcg agggcgagcc gcccgggctt ggggatgcgc gaggcgaaca cgcggtcgag
      781 ccaggcgcgc gcgccgtggc cggcgacctc gtactttccg tagttggtcg tctcgtagag
      841 accgaccgcg ctgcgcaccg cctggcactc ggcgcgcacg atggggaagg cctcggaacg
      901 gcgataggtc ggcgtctcga caggctccat gccgggaggc gcaaaccaca gcgcattctc
      961 gaggccgtag ttggcgccca tgcaggcgcg cgccgctttc agctgctcgt agatcggcga
     1021 gcggcgcacc gggcgcgccg ccggcagctc ctcgttggga taggcgaggc gaaagcgccg
     1081 cgagtaattc tccggcactt tgatcgaggt gtatttgggc gtcgcgaacg ccccgaagcg
     1141 cgccacgtcc atcgagatga tgtcctggcc cgggtcgttc tcggccatcc agcgcgatag
     1201 cacgagcccg atgccgcctc cctggctgaa gcccgccatg acggcgcacg ccacccagta
     1261 gttcctcagc ccccgcacgg gtccgacgag cggattaccg tcgggcgcga aggtgaaggg
     1321 accgttcacg gccttgcgga tgccgacgct acccagagcc gggaagtgcc ggaagcccac
     1381 ctcgaagtag ggcgcgagcc gctcgaagtc ctcgggcagg agctgcatcg agaagtcgtc
     1441 cggagtcttg agcggcgacc agacgatgcc gtgcggctcg taggtgccga tgagtgctcc
     1501 gccgcgctcc tggcgcacgt agatctcgcc ggcgtagtcg gtggtgttga cgatctcgcg
     1561 cgcgcggccc ttcagctcgg ggagatcctc agtgatgaga tagtggtgct ccatcgcgag
     1621 caccggcagc tcgatgccga ccatgtggcc gacctcgcgc gcccagagcc ccgcgcagtt
     1681 gacgacatgc tcggcatgca ccgcgccctt gtccgtgacg acgtcccagg tgccgtcgcg
     1741 gcgggagctg agcgacagca cgcgggtaaa gcgctcgacg gaggcgccga gctgccgggc
     1801 cgccttggcg taggcgttgg tagtccctga cgggtcgcag tgcccgccgt cggcgcgcca
     1861 cagcgcgccg atgaagtggc tcgggtcgat gagcgggttg tactcgagcg cctgctcgag
     1921 cgagatcatc tcggtctcca tgccgagata gcgcgcgcgc gagcagatga gcctcaggct
     1981 gtcgagctcg ccctggttgg cggcgagcat gagcccgccg ttcggatgga gcccgcatga
     2041 ctggccggac agccgctcga tctcccggta gaggtcgatg gtgtatttct gcaggcgcga
     2101 gatgttggcc tcgccgttga aggtgtgcat gccgcccgcg gcatgccagg tggagccgga
     2161 ggtcagctcg cttctctcga tgaggagcac gtcgctccag ccgatcttcg cgaggtgata
     2221 gagaacactc gcgcccacca cgccgccgcc gatgaccacc gctcgcgcgg aatccttcat
     2281 gtcgagccat ctccccacaa ggcgctcacc gtcgcatccg cagccagggc gagcccgaag
     2341 ggcttcgagc tcgccacgag ccactcgcgc agatgccgcg ccgtggtggc gggcgtcagc
     2401 agcagcatcc ccggagtgag ccgggcgagg attgccggca cctgcaccat gattgtggca
     2461 gctgcgcttc ctgcggggaa cgcgcctgcg tcaaggtcga ggcggcacgc ccgcgcgagc
     2521 atcccgccaa cctgcgaacc cgtgagaaaa aacgccgcca gcgccgcgga caggtccacg
     2581 acggcggcat agccgccgca catctcccgc cagtgcccgg cgctctcgtc cgggggcgcg
     2641 ggaggcgcca gcaacagcca gcgtccgggg cgcacgttga gcgcgaggcg cccggagcgc
     2701 gcctcgatgc tgcctggggc ggggaggcgc aggcctcgct cggcggtgag ccgctgaaga
     2761 tctgccgccc ggtcccggaa cgtcgccacc tcgacgatgt ccgcccggca gtccgcaagg
     2821 acgatgccct caggcgcgga cacgagtgtt ctccggatcg agcatgtggg gactcacgat
     2881 ctcgagctcg gtcgtctccg cgtgaatggg cgaggtgccg acgagacgag taccgatgcg
     2941 ctgtcttccg gcggcgagca gcgcgagtcc cacccagccc tcgagctcaa cggacggagt
     3001 ggaggaggtg atgtaaccga gactcgcgct ccgcccctcc ggtgcgagca ggtgcatccc
     3061 attgcgcagc cgccgcccac ggtccacggg gcgcacgccg acgagctgca gccgatccgc
     3121 cgccgtcagt ccggggcggg ccgccagcga gcggccgacg aagtcgccgc tctttttcaa
     3181 catgcgctga aaccccaggt cgtcggcgct ggtattaccg ttgagctcgg cggtcgtgac
     3241 gtggcccttt tcg
//
LOCUS       DATLKL010000496         1682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_39642, whole genome shotgun sequence.
ACCESSION   DATLKL010000496 DATLKL010000000
VERSION     DATLKL010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39642"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="VM713_06645"
     CDS             <1..1638
                     /locus_tag="VM713_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HVH83976.1"
                     /translation="DSYQESMFCYTNNIPQKDGGTHLAGFRSALTRTLNDYVEKELNG
                     KKDKIPMTGDDAREGLTAILSVKLPDPKFSSQTKDKLVSSEVKGIVENAVGTKLQEFL
                     LEHPQEARLIVAKMVEAARAREAARKAREMTRRKGALDVAGLPGKLADCQEKDPALSE
                     IFIVEGDSAGGSAKQGRDRRTQAILPLKGKILNVERARFDKMLSSAEVGTLITALGTG
                     IGRDDFDVGKLRYHRICLMTDADVDGSHIRTLLLTFFYRQMPELIERGHIYIAQPPLY
                     KIKRGKQETYVKDDNELNQLLLNTALESAGLHVNSAAPPLAGSSLELLARKYMEVQAI
                     IKRWSRRYDDRLLEQLIYMPEVKPADFDRAEWLRGWTADLNGRLNALADGTRTYRLEV
                     RSAGAGQAARVIVHKTEHGTDSHKYLPREFFESAEYQRIAELARTLAGLIGEGAYVTR
                     GNERQEIGSFKEAMKWLFDQAKKGQSIQRYKGLGEMNPEQLWETTINPETRRLMQVKI
                     EDAVAADDIFTTLMGDQVEPRREFIEKNALSVSNLDV"
BASE COUNT          335 a          563 c          539 g          245 t
ORIGIN      
        1 gattcctacc aggaatcgat gttttgttac acgaacaaca ttccgcagaa ggacggcggc
       61 acgcacctcg cgggctttcg ctcggcactg acgcgcacgc tcaacgacta cgtcgagaag
      121 gagctcaacg ggaagaagga caagatcccg atgaccggcg atgacgcgcg cgagggcctc
      181 acggcgatcc tgtcggtgaa gctgcccgat cccaagttct cctcgcagac caaggacaag
      241 ctcgtgtcct ccgaggtgaa gggcatcgtc gagaacgcgg tgggcaccaa gctccaggag
      301 ttcctgctcg agcacccgca ggaagcgcgc ctcatcgtcg cgaagatggt cgaggcggcg
      361 cgcgcccgcg aggcggcgcg caaggcgcgc gagatgacgc gccgcaaggg cgcgctcgat
      421 gtggcgggcc tgcccggcaa gctcgccgac tgtcaggaga aggacccggc gctgtcggag
      481 atcttcatcg tcgagggcga ctccgccggc ggctcggcca agcaggggcg cgatcggcgc
      541 acccaggcga tcctcccctt gaagggcaag atcctgaacg tcgagcgtgc gcgcttcgac
      601 aagatgctct catcggccga ggtgggcacg ctcatcacgg cgctcggcac gggcatcggt
      661 cgcgacgact tcgacgtcgg caagctccgc tatcaccgca tctgtctcat gacggatgcc
      721 gacgtcgacg gcagccatat ccggactctg ctcctcacgt tcttctaccg gcagatgccc
      781 gagctcatcg agcgcggcca catctacatc gcacagccgc cgctctacaa gatcaagcgc
      841 ggcaagcagg agacctacgt caaggacgac aacgagctca atcagcttct gctcaacacc
      901 gcgctcgaga gcgcagggct ccacgtgaac tccgcggctc cgccgctcgc gggctcctcg
      961 ctcgagctcc tggcgcgcaa gtacatggag gtccaggcga tcatcaagcg ctggtcgcgc
     1021 cgctacgacg accggctcct cgagcagctg atctacatgc cggaagtgaa gccggcggac
     1081 ttcgatcgcg ccgagtggct gcgcggctgg accgccgatc tcaacgggcg cctcaacgcg
     1141 ctcgccgacg gcacgcgcac ctatcgcctc gaggtgcgct cggcgggcgc cggccaggcc
     1201 gcgcgcgtca tcgtgcacaa gaccgagcac ggcacggaca gccataaata cctgccgcgc
     1261 gagttcttcg agtctgccga gtaccagcgg atcgccgagc tcgcgcgcac gctcgccggg
     1321 ctcatcggcg agggcgccta cgtgacccgc ggcaacgagc gccaggagat cggctccttc
     1381 aaggaagcga tgaagtggct gttcgaccag gccaagaagg gccagagcat ccagcgctac
     1441 aaaggcctcg gcgagatgaa tccggagcag ctgtgggaga ccacgatcaa cccggagacc
     1501 cgccgactca tgcaggtgaa gatcgaggac gcggtcgctg ccgacgatat cttcacgacc
     1561 ctcatggggg atcaggtcga gccacggcgc gagttcatcg agaagaacgc gctgtcggtg
     1621 agcaacctcg acgtgtgaga gaggctcgac ttatgttcca cgtggaacac cctcgctcct
     1681 ca
//
LOCUS       DATLKL010000497         1747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_62237, whole genome shotgun sequence.
ACCESSION   DATLKL010000497 DATLKL010000000
VERSION     DATLKL010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62237"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VM713_06650"
     CDS             <1..259
                     /locus_tag="VM713_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83977.1"
                     /translation="LMAGTRAQFPQILATTVPYWSEIERMTLRRAPLPAFAPHSEAAA
                     IYAALWAEMEERMAGANEALGGAAARPAAPGIARAPLGARL"
     gene            complement(256..1224)
                     /locus_tag="VM713_06655"
     CDS             complement(256..1224)
                     /locus_tag="VM713_06655"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HVH83978.1"
                     /translation="MRGPLGLGFVFLNVLLQQLGLPVPVVPTLVVAGAVAASGRMSGY
                     ALYALAVSACVIADSTWYTAGRRYGGRVMSLLCRVSLSPDSCVSQTQGAFERWGARAL
                     VFAKFVPGLALIAPPHAGATRMQFGRFIIFSAIGAGLWVGAALLGGALLRPQIERLLP
                     QALQVGGTAAGVLVLLLAAYVAYRWWERRRFLAALDMARITVGELRQQMDAGAAPVIV
                     DVRSPTAQTLERHRIPGAVNVPVQDAARHLSELPRDREIILYCTCPNEASAAKVARLL
                     MNAGFRRVRPLKGGLEAWIEAGYSVEAFSLPAHTGTDPSPTPQPSS"
     gene            complement(1304..>1747)
                     /locus_tag="VM713_06660"
     CDS             complement(1304..>1747)
                     /locus_tag="VM713_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HVH83979.1"
                     /translation="AFTGSFRTDNELRGTVANPVNESRLRAAITSHMQSIGVQLAKEN
                     ADCLVGYGIGSSLVVDPGYPAGAYPYWGYGYWGWGGPYWGPWGWGGGPYVYRQGMIGI
                     DLYDGKTHEALWHASVDQSLYGATGADAEQKIDAAVTALFAHFPT"
BASE COUNT          290 a          610 c          624 g          223 t
ORIGIN      
        1 cctgatggcg ggcacccgtg cgcagttccc gcagattctc gcgacgaccg tcccgtactg
       61 gagcgagatc gagcgcatga cgctgcgccg cgcgccgttg ccggcgttcg cgccgcacag
      121 cgaggcggca gcgatctacg cagctctctg ggcggagatg gaggagcgca tggccggagc
      181 caacgaagct ctgggcggcg cggctgcccg gcccgcggct ccggggatcg cccgcgcgcc
      241 tctgggagcc cgcctctagg agctcggttg cggggtgggg gaaggatccg tcccggtatg
      301 cgccggaagg gagaacgcct cgaccgagta gcccgcctcg atccaggcct cgagcccgcc
      361 cttgagcggg cgcacccgcc gaaagccggc gttcatgagc agccgcgcga ccttggccgc
      421 ggaggcctcg ttcgggcagg tgcagtagag aatgatctcg cgatcccgcg gcagctcgct
      481 caggtggcgc gcggcgtcct gcaccggcac gttcacagcg ccaggaatgc ggtgtcgctc
      541 gagcgtctgc gcggtcggcg agcgcacgtc gacgatcacc ggcgcggctc ccgcgtccat
      601 ctgctggcgc aactccccga cggtgatgcg cgccatgtcg agcgccgcca ggaaacgccg
      661 ccgctcccac cagcggtacg cgacgtaggc ggcgagcagc agcaccagaa ccccggcggc
      721 ggtgccaccg acctgcagag cctgcggcag cagccgctcg atctgggggc gcagcagcgc
      781 accgccgagc agcgccgcac cgacccagag gcccgcgccg attgcgctga agatgatgaa
      841 tcggccgaat tgcatgcgcg tcgctccggc gtgcggcggc gcgatgagtg cgaggccggg
      901 tacaaacttc gcaaatacga gcgccctggc gccccagcgc tcgaatgcgc cctgcgtctg
      961 gctcacgcat gaatcgggcg agagcgacac ccggcacagg aggctcatga cgcggccgcc
     1021 gtagcggcgg cccgccgtgt accaggtgct gtcggcgatc acgcaggcgc tgaccgcaag
     1081 cgcataaagc gcgtacccgg acatccggcc gctggccgcg acggcaccgg cgaccacgag
     1141 ggtgggtacc acgggcacgg gcagccccag ctgctgcagc agaacattca ggaagacaaa
     1201 gccgagaccg agggggccgc gcaccgtgcg atcttgcgcc gtgtgccgcg gagcgacaag
     1261 gacaccgacg ggcgagcggc ggatcccccg aacggcagga aagtcaggtc gggaagtggg
     1321 cgaagagggc ggtcaccgcg gcgtcgatct tctgctccgc gtccgcgccc gttgcgccat
     1381 agaggctctg gtcgaccgag gcgtgccaga gcgcctcgtg ggtcttcccg tcatagagat
     1441 cgatgccgat cattccctgg cggtagacgt agggaccgcc accccagccc cacggtcccc
     1501 agtacggccc accccagccc cagtagccgt agccccagta agggtaggcg ccagccggat
     1561 agccggggtc gacgaccagg ctcgagccga taccgtagcc gaccaggcag tcggcgttct
     1621 ccttcgccag ttgcacgccg atcgactgca tgtgcgaggt gatggcggca cgcaggcggc
     1681 tctcgttcac gggattggcc accgtgccgc gcagctcgtt atcggtccgg aaggagccgg
     1741 tgaaggc
//
LOCUS       DATLKL010000498         2013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_163686, whole genome shotgun sequence.
ACCESSION   DATLKL010000498 DATLKL010000000
VERSION     DATLKL010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163686"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VM713_06665"
     CDS             complement(<1..357)
                     /locus_tag="VM713_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVH83980.1"
                     /translation="MAASGAELVLPPAADSGAHPVKYDVRHLDFYYGESQALKDINLT
                     LADRRITAFIGPSGCGKSTLLRVFNRMYALYPGHRATGDVRLDGEDILAATVDVAALR
                     YRVGMLFQKPTPFPMSI"
     gene            complement(357..1205)
                     /gene="pstA"
                     /locus_tag="VM713_06670"
     CDS             complement(357..1205)
                     /gene="pstA"
                     /locus_tag="VM713_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HVH83981.1"
                     /translation="MSRRFLRRLTNFVALSLSGLATALGLFFLGAILWTLLSRGLAGM
                     SGTLFTAMTPPPGGSGGLLNAIYGSVVMTVVGILLGGPVGMLAGTYLAEYGRHARLSA
                     VIRFVNDVLLSAPSIIVGLFVYELLVIRMGHFSALAGAVALAIIAIPVTVRTTEDMLN
                     LVPQGMKDASTAMGAYPWRTITDVIYPAARSGIVTGLLLAVARISGETAPLLFTALNN
                     QFWSTNLDAPMANLPVVIFQFALSPYPNWQQLAWSGALLITLSILILSITARFVVARL
                     GPGGQR"
     gene            complement(1202..>2013)
                     /gene="pstC"
                     /locus_tag="VM713_06675"
     CDS             complement(1202..>2013)
                     /gene="pstC"
                     /locus_tag="VM713_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HVH83982.1"
                     /translation="HFKLGFLTTSTWNPVTERFGALAAVYGTIVTSPIALLITIPVSV
                     GIAIFLTELAPAWPKRPVGIAIELLAAVPSIIFGIWGLFVLAPILQRHVQPWLIEALG
                     PLPIVGRLFQGPPFGIGILTAGIVLAIMVVPFVSAVTRDVFETVPDVLKESGYGLGAT
                     TWEVIWQVVVPYARTGMIGGIMLGLGRALGETMAVTFVIGNAHRIGSSLLAPGTTISA
                     AIANEFTEAVGDLYTSSLVALGALLFLITFSVIAAARFMLLRLERRALSSV"
BASE COUNT          402 a          644 c          706 g          261 t
ORIGIN      
        1 gatcgacatc gggaagggcg tgggcttctg gaacaacatg ccgacccggt agcgcagcgc
       61 cgccacgtcc acggtcgccg ccaggatgtc ctcgccgtcg agccgcacat ccccggtggc
      121 ccgatgcccg gggtaaagcg cgtacatacg gttgaagacc cgcagcagcg tcgacttgcc
      181 gcaacccgag gggccgatga aggcggtgat acgccggtcc gcgagcgtga gattgatgtc
      241 cttcagcgcc tgagactcgc cgtagtagaa gtccaggtgg cgcacgtcgt atttgaccgg
      301 gtgcgcgccg ctgtccgcgg cgggcggcag gaccagctcg gcgcccgagg cagccatcaa
      361 cgctgccccc cgggaccgag ccgcgcgacc acgaagcgcg ccgtgatcga gaggatcagg
      421 atcgagaggg tgatgagcag cgcccccgac caggcgagct gctgccagtt tgggtacggg
      481 ctcagcgcga actgaaagat gacgaccgga aggttcgcca tcggcgcgtc gaggttcgtg
      541 ctccagaact gattgttgag cgccgtgaag agcagcggcg cagtctcgcc gctgatgcgc
      601 gcgacggcga gcagcagtcc ggtcacgatc ccgctgcgcg cggccgggta gatgacgtca
      661 gtgatggtgc gccagggata ggcgcccatg gcggtggagg cgtccttcat cccctgcggc
      721 accaggttca gcatgtcctc ggtcgtacgc accgtcaccg gaatggcgat gatcgcgagc
      781 gccaccgcgc ccgcgagcgc ggaaaagtgt cccatgcgga tcaccagcag ctcgtacacg
      841 aacaggccga cgatgatcga cggcgccgac agcagcacat cgttcacgaa gcggatcacc
      901 gcggagaggc gcgcatggcg cccgtactcg gcgaggtaag tgccggcgag catgccgacg
      961 gggccgccga ggaggatgcc gaccacggtc atgacgacgc tgccgtagat cgcgttgagg
     1021 agtccgccgc tgccgccggg cggcggagtc atggcggtga acaaggtccc tgacatgccg
     1081 gcgagcccgc ggctcaagag cgtccagagg atcgcgccga gaaagaacag accgagcgcc
     1141 gtggcaaggc ccgacaggct gagcgccacg aagttggtga gcctgcgcag gaagcggcga
     1201 ctcataccga ggagagcgca cggcgctcga gacgtagcag catgaaacgc gccgccgcga
     1261 tcaccgagaa cgtgatgaga aagagcagcg cgccgagcgc gaccagagac gaggtataga
     1321 ggtcgcccac tgcctcggtg aactcgttgg cgatcgccgc cgaaatggtc gtgccgggag
     1381 cgaggagcga agagccgatg cggtgagcgt tgccgatgac gaaggtcacc gccatggtct
     1441 caccgagcgc acgtcccagg cccagcatga tcccgccgat catgccggtg cgcgcgtacg
     1501 gcacgaccac ctgccagatg acctcccaag tggtggcgcc gagcccgtag ccggactcct
     1561 tgagcacgtc cggcacggtc tcgaacacat cacgcgtgac cgcggagacg aacggcacca
     1621 ccatgatggc gagcacgatg ccggcggtca gtatgccgat gccgaagggt ggcccctgaa
     1681 agagcctgcc gacgatgggc aggggcccga gggcctcgat gagccacggc tggacgtgcc
     1741 gctgcaggat cggcgccagc acgaacagcc cccagatgcc gaagatgatg ctcggcaccg
     1801 ccgccagcag ctcgatcgcg atccccaccg ggcgcttggg ccaagcaggc gcgagctcgg
     1861 taagaaaaat agcgattccc acactgacag gaattgtgat caggagcgca atgggggagg
     1921 tcactatcgt cccgtagacc gccgccagcg cgccgaaccg ctcggtcacc gggttccacg
     1981 tcgaggtggt gaggaacccc agcttgaagt gcg
//
LOCUS       DATLKL010000499         3293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_96190, whole genome shotgun sequence.
ACCESSION   DATLKL010000499 DATLKL010000000
VERSION     DATLKL010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96190"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(523..2058)
                     /locus_tag="VM713_06680"
     CDS             complement(523..2058)
                     /locus_tag="VM713_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HVH83983.1"
                     /translation="MATPSAPDVRNRAAITVCVILATLMQALDTTIANVALPYMQGSV
                     AASQDEIAWVLTSYIVAAAIMTPPTGYLAGRFGLKRLFLVSVAGFTCASMLCGMAQSL
                     VEIVLSRVLQGLFGAALVPLSQTVLLDINPKERQGSAMALWGVAVMAGPVLGPVLGGW
                     LTEAYSWRYVFYINLPIGALTFVGMSVFLPNARGNARAKLDWFGFATLSLAIGALQVL
                     LDRGEELDWFGSGEIVVEAIIAASAFYLFLVHTFTAREPFVRPSLFRDRSFTAGILFV
                     AIVGLTYYASLALQPPYLQNLMNYPIVSAGIVLGPRRIGTMAAMMIVGKLIGRADIRL
                     LLGIGLTLTAWSFYAMTGWTPDVSQSTIIVVGVVQGVGLGFLFVPLSVVTLSTLPSNL
                     TAEGSGLYSLSRNIGSSVGISVVNSLLTINTQVNHAEIAQHVTPVNRVFADPMIVQFW
                     NPVTAAGRAALDAVVTQQAQIIAYIDDYKLLMIATIAVIPLLIVFKKPSRGQERDYAA
                     VLE"
     gene            complement(2058..3245)
                     /locus_tag="VM713_06685"
     CDS             complement(2058..3245)
                     /locus_tag="VM713_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HVH83984.1"
                     /translation="MADEEVVSLRAAPEGVPAPLRDPAQGRAPIKRFRAPDEAPRAPR
                     RGWLRRVLFLLLPIALIAGSYWYVTGGRVMSTDDAFVEAEKVGVSTDVSGIVKEIDVS
                     ENQRVDAGQVLYRLDDLPFRLALQRAEAQAGIVRNDLNALKANYQDMQAQIRQAQYDI
                     EYYDREFRRQQMLVIRSVVSQASFDTARRNLQNAQQKLASLNQQLAAIAANLNSDPSI
                     PVEKHPRYLDALAQRDEAARQLDHTVVKAPFAGTVTNVPSIAPGKYLQASTTAFYLVA
                     TDHVWVDSNPKETELTYVHPGQPVAVIVDMYPGIEWHGTVESISPAAAQEFSLLPAQN
                     TSGNWVKVVQRIPMRVRVDTSDKSLPPLRAGMSVEVDVDTGHTRGLPHFLTALLGPAR
                     EES"
BASE COUNT          616 a          998 c         1111 g          568 t
ORIGIN      
        1 aaaaatcata ggcgggtcgc ttaattgggg acgcgcgaga aagcgggccc cggcgctcgg
       61 ccgaaagagc gccggggccg tcggctcgcc cttcggggcg ccccggggga gggagcgtgc
      121 gagggcgagc ctgactcgat cgtgctttca tcattcggcg ctgcgagtct caataatgct
      181 ccatgtacgc cttctcgtga ggcgtgcgga gggaagctcg cgcaagttcg tctggcggca
      241 gcatcctcgg cgggcctgaa accgccgaca aaatttcgag gccgagcaaa agcgcggcga
      301 caatgagcca tgcggcataa aggccgctgg gggcaccgga actgaacaag aatgagcccg
      361 gccggggtcg atgctgtcgg ctgagcggcg gggattaatc gcggagccag ctggtatggt
      421 tgcgcgccgg tgctagttgt ttgccggtcg ccggctcgcc cctcggggca ccccgtgggg
      481 agtgagcgta tgagggacga gccagaatca atcgtgctct catcattcga gcaccgccgc
      541 gtaatcccgc tcttgcccgc gggatggctt tttgaagaca atgagcagcg ggatcaccgc
      601 gatcgtcgcg atcatcagca gtttgtagtc gtcgatatag gcaatgatct gcgcctgctg
      661 cgtgacgacg gcgtcgagtg cggcgcgtcc cgcggctgtg accgggttcc agaactgtac
      721 gatcatcggg tccgcgaaca cccggttgac gggggtgacg tgttgcgcga tctcggcgtg
      781 gttcacctgc gtgttgatcg tcaacaggct gttcaccacc gagatgccga cgctcgaacc
      841 gatgttgcgt gacaggctgt acaggccgga gccttccgcg gtcaggttcg acggcagcgt
      901 cgacagtgtc acgacgctga gcggcacgaa gaggaagccc aggccgacgc cctgaacgac
      961 accgacgacg atgatcgttg actgcgaaac gtccggcgtc caaccggtca tcgcatagaa
     1021 cgaccacgcc gtcagggtga ggccgatgcc gagaagcagg cgaatatccg cccgaccgat
     1081 cagctttccg acgatcatca tcgcagccat ggtgccgatg cgtcgcggcc ccagcacgat
     1141 gcccgcgctg acgatcgggt aattcatgag attctgaaga taaggaggct gcagggccag
     1201 cgaggcgtag taggtgaggc ctacgatcgc gacgaagaga atgccggccg tgaagctgcg
     1261 gtcgcggaac agcgaaggcc tgacgaaggg ctcgcgcgcg gtgaaggtat ggacgaggaa
     1321 caaatagaac gccgacgccg caatgatggc ctcgacaacg atctcgccgg agccgaacca
     1381 gtcgagctct tcgccgcggt ccagcaggac ctgcagagcg ccgatcgcta ggctcagggt
     1441 ggcgaaaccg aaccagtcca gcttggcgcg ggcgtttcct cgcgcgttcg gcaggaagac
     1501 gctcatgccg acgaaggtga gcgctccgat cggcaggttg atgtagaaga cgtagcgcca
     1561 gctgtaggct tcggtcagcc agccgccgag caccggccca aggacgggac ccgccatcac
     1621 cgcgacgccc caaagcgcca ttgccgatcc ttgccgctct ttgggattga tatcgagcag
     1681 cacggtctgc gagagtggga cgagcgccgc gccgaacagg ccttgcagga cccgggacag
     1741 cacgatctcg accagcgact gcgccatgcc gcacagcatc gaggcgcagg tgaagccggc
     1801 gacggagacg aggaaaaggc gcttgagccc gaagcgcccg gcgaggtagc cggtcggcgg
     1861 cgtcatgatc gcggcggcaa cgatatatga ggtcagcacc caggcgatct cgtcctgact
     1921 ggccgcgaca ctgccctgca tatagggcag ggcgacattg gctatggtcg tgtcgagtgc
     1981 ctgcatgagg gtggcgagga taacgcagac cgtaattgcc gcccgattgc ggacatccgg
     2041 cgcgctcggc gttgccatca tgactcctcc cgagcgggac cgagcaacgc ggtcaagaag
     2101 tgcggcaagc cccgtgtatg gccggtgtcg acgtcgacct cgacgctcat cccggcgcgc
     2161 agcggcggca ggcttttgtc gctcgtgtcg acacgtacgc gcatgggaat gcgctgcacg
     2221 actttcaccc agttcccgct agtgttctgc gccggcagga gcgagaactc ctgtgccgcc
     2281 gcagggctga tgctctcgac tgtgccgtgc cattcgatgc ccggatacat gtccactatc
     2341 acggcgaccg gttgcccggg gtgtacatag gtcaattccg tctctttcgg gttcgagtcg
     2401 acccagacgt gatccgtggc gacgaggtag aacgcggtcg tcgaggcttg gagatactta
     2461 ccgggggcaa tcgagggcac gttcgtcacg gtgccggcga acggcgcttt gacgacggtg
     2521 tggtcgagct ggcgcgccgc ttcgtcacgt tgcgctaggg catcgagata gcgcgggtgc
     2581 ttctcgactg ggatgctcgg gtcgctgttg aggttggcgg caatcgcggc gagctgctga
     2641 ttgagagagg ccagcttctg ttgcgcgttt tgcaggtttc ggcgagccgt gtcgaaactt
     2701 gcttgagaca cgacgcttcg gatcacgagc atctgctgac ggcgaaattc tcggtcatag
     2761 tattcgatgt cgtactgcgc ctgtctgatc tgcgcctgca tatcctggta gttggccttg
     2821 agcgcgttca gatcgttccg gacgatgccg gcctgcgcct cggcgcgctg cagcgcgagc
     2881 cggaacggca aatcgtcgag gcggtagagg acctggccag cgtcgacccg ctggttctcc
     2941 gagacgtcaa tctccttgac gatgccggaa acgtcggtcg agacgccgac cttttcggct
     3001 tcgacaaacg cgtcatccgt cgacatcact cgaccgccgg tgacgtacca ataactgccg
     3061 gcgatcagcg cgatcggcag gagcagaaac aacacccggc gcagccagcc ccggcgcggc
     3121 gcccgcggcg cctcatcggg cgcccggaac cgctttatcg gggctcgtcc ttgcgcgggg
     3181 tcgcgtagtg gcgctggcac cccttccggg gccgcacgca gcgaaaccac ctcctcgtca
     3241 gccaccaaca ggcctttcga cgatgatcac accagtcgcc agggtattga cgg
//
LOCUS       DATLKL010000500         2443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_118740, whole genome shotgun sequence.
ACCESSION   DATLKL010000500 DATLKL010000000
VERSION     DATLKL010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118740"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VM713_06690"
     CDS             <1..444
                     /locus_tag="VM713_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83985.1"
                     /translation="AVDTTAATTPDAAPARAAAAASAARVTPKGDKPAAAGSAAPSKS
                     AAASDASGPGKPGARGPKGPDRVELETTEITGNRELPKVLYIVPWKRSDLGDLVSRPA
                     NSLLDEVLQPIDRDVFLRENRYYDALKPDVAGPKADTVPGSGDKP"
     gene            562..1212
                     /locus_tag="VM713_06695"
     CDS             562..1212
                     /locus_tag="VM713_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVH83986.1"
                     /translation="MQIWNLIVRFFQGGGDFMYPIAIVLVFGLAIALERWIYLAHTAA
                     RNRGLWNEIVPLLSQGNFRGVAQLTSKSPSAIGHILNYGLARIQSARRRDDIEKAMEE
                     SLMEVVPRLEKRTHYLATFANLATLLGLLGTVMGLIRAFAAVATVNPAEKANLLSASI
                     SVAMNCTAFGLMTAVPLLFIHAWLQTKTTDVIDSLEMASVKFLNAITERQTAPAAA"
     gene            1253..1834
                     /locus_tag="VM713_06700"
     CDS             1253..1834
                     /locus_tag="VM713_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVH83987.1"
                     /translation="MRRSYQIRKLERHARKPAELLLVPMIDIFTVLVTFLLMTAVFSR
                     TVILQLNLPPPNTTWKEPPPGLQLEVMVRKNLLQVADRNTGPLATLPNTASGYDYDGL
                     TEYLKRVKAKFPDKTDASILLESDTQYDTLVQVMDRVRVFEVGQGISTVQAELFPDIS
                     IGDAPATTEASAGAAPVAGAPAASAPRPGGGHS"
     gene            1831..2355
                     /locus_tag="VM713_06705"
     CDS             1831..2355
                     /locus_tag="VM713_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVH83988.1"
                     /translation="MSMSNRARRMAQHHLRHKADAQLNLIPLIDILSVMVAFLLVYST
                     EVEVIQNSKGIEIPESVAQTAPKQSVVVMITRDQLFVQGEYIAAVKEIEASPGPLIAP
                     LSAALKRPMLVGQATTEQDISQREITIMADKVLPYEVLKRVMQTCTDADYGKISLAVI
                     QKEKPVAAGQVKTS"
BASE COUNT          434 a          817 c          796 g          396 t
ORIGIN      
        1 gctgtcgaca cgaccgccgc cacgacgccg gatgcggcgc cggcgcgagc cgcggccgct
       61 gcctccgcgg cgcgcgtgac gccaaagggc gacaagcctg cggcggccgg cagcgccgcg
      121 cccagcaagt ccgcggccgc gagcgatgcc tcgggaccgg gaaagcccgg cgcgcgcggc
      181 ccaaagggac cggatcgggt ggagctcgag acgaccgaga tcaccggcaa ccgcgagctc
      241 cccaaggtgc tgtacatcgt gccgtggaag cgctccgatc tcggcgatct cgtcagccgg
      301 cccgccaaca gtctgctcga cgaggtgctg cagccaatag atcgcgacgt gttcttgagg
      361 gaaaatcgct attacgatgc gctgaagccc gatgtggcgg gcccgaaggc ggacacagtg
      421 ccgggttctg gcgacaagcc gtgaggccag gcccagagcc tctcaggatg agaggtgtat
      481 cgcagctttt ccgtcttatg tcgttcagtt tgcgcttgct tgcgtcccca ggtgtggggg
      541 tttcttgatc gggagagtca gatgcagatc tggaatctga tcgtgcgttt ctttcagggc
      601 ggcggggact tcatgtaccc gatcgccatc gtgttggtgt tcggactggc catcgccctc
      661 gagcgctgga tctatctcgc acacaccgcc gcgcgcaatc gcggcctgtg gaacgagatc
      721 gtcccgctgc tgtcgcaggg caacttccgc ggcgtcgcgc agctgacctc gaagtcgccg
      781 tcggccatcg ggcacatcct caactacggc ctggcgcgca ttcaatcggc ccgccggcgc
      841 gatgacatcg agaaggcgat ggaagagagc ctgatggagg tggtgccgcg gctcgagaag
      901 cgcacgcact atctggcgac ctttgccaat ctggcgacgt tgctcgggct gctcggcacg
      961 gtgatgggcc tgatccgcgc cttcgccgcg gtggccaccg tgaacccggc ggagaaggcc
     1021 aacctgctgt ccgccagtat ctcggtggcg atgaactgca ccgccttcgg cctgatgacc
     1081 gcggtgccgc tgctgttcat tcacgcgtgg ctgcaaacca agaccacgga cgtgatcgac
     1141 agcctcgaga tggcctcggt caagttcctc aacgcgatca ccgagcggca gacggcgccc
     1201 gcggccgcct gagctcgagc gcccgcgcgc acctaacggc cgaaggaacc gcatgcgccg
     1261 ttcttatcag atccgcaagc tggagcgcca tgcgcgcaag ccggccgagc tgctgctcgt
     1321 tccgatgatc gacatcttca cggtgttggt tacgttcctg ctgatgaccg cggtgttctc
     1381 ccgcaccgtg atcctgcagc tgaacctgcc gccgcccaac acgacctgga aggagccgcc
     1441 gccggggctg cagctcgaag taatggtgcg caagaacctg ttgcaggtcg ctgaccgcaa
     1501 caccggcccg ctcgccacgc tccccaatac ggcgagcggc tacgattacg acggcctgac
     1561 ggagtacctg aagcgcgtga aggccaagtt ccccgacaag accgacgcca gcatcctgct
     1621 cgagtccgat acccagtacg acacgctggt gcaggtcatg gaccgcgtgc gcgtgttcga
     1681 ggtggggcag ggcatcagca ccgtgcaggc ggagctgttc cctgacatct ccatcggcga
     1741 tgcacccgcg accaccgagg catccgccgg cgctgctccg gtcgccggcg cgcccgcggc
     1801 cagcgcgccg cgacccggcg gaggtcactc atgagcatgt cgaaccgcgc ccggcgcatg
     1861 gcgcagcatc acctgcggca caaggccgac gcccagctca acctcatccc gctcatcgac
     1921 atcctctcgg tgatggtggc gttcctgctg gtgtactcga ccgaggtcga ggtgatccag
     1981 aactccaagg gcatcgagat tccggagtcg gtcgcgcaga cggcgccgaa gcagtcggtg
     2041 gttgtgatga tcacccgcga ccagctgttc gtgcagggtg agtacatcgc cgcggtgaag
     2101 gagatcgagg cttcgcccgg gccgctgatc gcgccgctct ccgccgcgct caagcgcccg
     2161 atgctggtcg gccaggcgac gaccgagcag gatatctcgc agcgcgagat caccatcatg
     2221 gcggacaagg tgctgccgta cgaagtcctc aagcgcgtca tgcagacctg caccgacgcc
     2281 gactacggca agatctcgct cgccgtgatc cagaaggaaa agccggtcgc ggccggccag
     2341 gtgaagacga gttagcgcgc atgaatctcg ctccctacta ccgcgaatac gagctgccct
     2401 gggaaggcga cccggaatcg agcgagcgct ttcgcaagat cct
//
LOCUS       DATLKL010000501         1526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_152677, whole genome shotgun sequence.
ACCESSION   DATLKL010000501 DATLKL010000000
VERSION     DATLKL010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152677"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VM713_06710"
     CDS             complement(<1..1283)
                     /locus_tag="VM713_06710"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HVH83989.1"
                     /translation="MTLALEAGVTQAIGQAASSDGPAPASTLPMLLGAQYTFIEQWQT
                     ALESPYQGPLSLRPDGDRQPTHTIGVYTGWAPVRWGQLYFDAEKFMGAGVSGATGLGG
                     LTNGDVIREGVTGLKKQFYVARAYARFILPLADEVSDVARGQDQLPGTEAARRLELKA
                     GQMSAADDFDRNRYANSARTEFLNWSLWNNTAWDFAANTRGYTDGVVLGYVSPVWSLR
                     YGLYRMPLYANGQTMQTLLRAREQDLELTLSPPGPSTVVRLLAYYNTARMGDYAQALA
                     IAAAAGTVPNVAADDHEGRHKTGYGLNLEQPLADNGDTGLFLRLGWQDGKREDFAFTE
                     VDRQASAGAQLAGIHWARGEDRLGVGVVLEGLSGVHRAYLAAGGSGFLLGDGRLSYAH
                     EQILEMYYRAGWTWSCGQGALQAQLSPDFQYIQNPG"
     gene            complement(1345..>1526)
                     /locus_tag="VM713_06715"
     CDS             complement(1345..>1526)
                     /locus_tag="VM713_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH83990.1"
                     /translation="NYRYKAGVVTYLEVVATENAALVARLAAVDIETRRISSTVLLVR
                     ALGGDWRTARPAGVR"
BASE COUNT          230 a          514 c          540 g          242 t
ORIGIN      
        1 cccgggttct ggatgtactg gaagtcgggg ctcagctgcg cctgcagtgc gccctgaccg
       61 catgaccacg tccagccggc gcggtagtac atctccagga tctgctcatg ggcatagctc
      121 aggcggccat cgccgagaag aaatcctgac ccgccagccg cgagataggc gcggtgcacg
      181 ccggacaggc cttcgagcac gactccgact cccaggcggt cctcgccacg cgcccagtga
      241 atgccggcga gctgggcgcc ggcgctcgcc tgccgatcga cctcggtgaa cgcgaagtcc
      301 tcgcgcttgc cgtcctgcca gccgaggcgc agaaacaggc cggtatcgcc gttgtcggcg
      361 agcggctgtt cgaggtttag cccgtagccg gtcttatgcc gtccctcgtg gtcgtcggcg
      421 gccacgttcg ggaccgtgcc ggcggcggcg gcgatcgcga gcgcctgggc gtagtcgccc
      481 atgcgcgcgg tgttgtagta ggcgagcagc cgtaccactg tgctcggacc gggaggggag
      541 agcgtcaact cgagatcctg ctcgcgcgcc cgcagcagcg tctgcatcgt ctgcccattc
      601 gcgtagagtg gcatccgata caggccgtac ctgagcgacc ataccgggct cacgtagccg
      661 agcacgacgc cgtcggtgta gccgcgggta ttggcggcga agtcccacgc ggtgttattc
      721 cacagtgacc agttgaggaa ctcggtgcgc gccgagttgg cgtagcggtt gcgatcgaag
      781 tcatcggccg ccgacatctg tcccgccttg agctcgagac gtcgcgccgc ctcggtgccc
      841 ggcagctgat cctggccccg ggccacgtcg ctcacctcat ccgcgagcgg caggatgaag
      901 cgggcatagg cgcgcgcgac gtagaactgt tttttgagac cagtgacacc ctcgcggatc
      961 acgtcgccgt tggtgagccc gccgagtccg gtcgcgccgc tgacgcctgc gcccatgaat
     1021 ttctcggcgt cgaagtacag ctgaccccag cggaccggag cccagccggt gtagacaccg
     1081 atggtgtggg tgggctggcg atcaccgtcg gggcggaggc tgagcggtcc ctggtagggc
     1141 gattcgagcg cggtctgcca ttgctcgatg aacgtatatt gcgcaccgag cagcatgggg
     1201 agagtgctgg cgggcgccgg gccgtcgctc gaggccgcct gaccgattgc ctgcgtcacg
     1261 ccggcctcga gcgccagggt caccgtgagg caggccgcga cgcgccgccg tccggcgcga
     1321 ggggaatgcg cgggcggcgg cacgctagcg gacgccggcc ggccgagcgg tgcgccagtc
     1381 accgccgagc gcccgcacca gcagcaccgt cgagctgatg cgccgcgtct cgatgtcgac
     1441 cgcggcgagc cgcgcgacga gcgccgcgtt ctccgtcgcg acgacctcga ggtaagtcac
     1501 taccccggcc ttgtagcgat agttgg
//
LOCUS       DATLKL010000502         2821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_186455, whole genome shotgun sequence.
ACCESSION   DATLKL010000502 DATLKL010000000
VERSION     DATLKL010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186455"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2050)
                     /locus_tag="VM713_06720"
     CDS             complement(<1..2050)
                     /locus_tag="VM713_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HVH83991.1"
                     /translation="MAETQVQARNGMRLTAAVSRALRESAVIALAIVALTLFVALASY
                     SPDDPGFSYTGNAAPIHNRIGVAGAWLADVLFFLFGWPAFLLPVVLAIAAWGLQRRVR
                     GLSISRVNTVVRVAGFVLLLAASCGLTTLHWEAGALRQTAGGVVGGLVGKGLAAWLDF
                     LGATLLLIAAWMAGISVAFHVSWLAVMDRLGGLTWGGIGWVRARWASRKDMAEGRERR
                     QARKEAVETEQKKAATRAPPRIEPPLPVVEKSARVERERQVPLFDPPKAKELPPLKLL
                     DDPPAREPLYSAEALEALSRLVEMKLKDFGIDAEVVAVQPGPVVTRFELRPAPGVKAS
                     QITALSKDLARALSVLSVRVVEVIPGKSVMGLEIANEKREVVTLGEIIRSRAYDEMHS
                     PLALALGKDIGGQPVVADLSRMPHLLIAGITGSGKSVALNAMVLSLLYKSTAEHVRLI
                     MIDPKMLELSVYEGIPHLLAPVVTDMKQASNALRWSVVEMDRRHQLMAALGVRNIAGF
                     NRRVKDAIDAGKPIRDPVMMARAANDPTIDQRQIEDLTPLPYIVVFIDEFADLMMIVG
                     KKVEELITRLAQKARASGIHLVLATQRPSVDVITGLIKANIPCRIAFQVSARVDSRTI
                     LDQMGAETLLGHGDMLYLPPGTSMPTRVHGAFVSDQEVHRVVDALKSPTPPNYIEEVL
                     S"
     gene            2120..>2821
                     /locus_tag="VM713_06725"
     CDS             2120..>2821
                     /locus_tag="VM713_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020796668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH83992.1"
                     /translation="MTSTKSKHSRLIILGSGPAGYSAAVYAARANRAPLLISGMAPGG
                     QLMTTTDVDNWPGDVEGLQGPALMERMRRHAERFATEIVSDHLVAVDLATRPFSLTGE
                     ADRYSCDALIIATGASARYLGLPSEERFRGRGVSACATCDGFFFRDQRVAVVGGGNTA
                     VEEALYLAHIARHVTLVHRRDKLRAERILQDRLFREVQAGKMSVVWNHTVDEVLGNEQ
                     GVSGVRLRALPAGAPQ"
BASE COUNT          457 a          992 c          942 g          430 t
ORIGIN      
        1 cggacagaac ctcctcgatg taattcggcg gcgtcgggga tttcagtgca tcgaccaccc
       61 ggtgcacttc ctgatcggag acgaaggcgc cgtgcacccg ggtcggcatg gaggtgccgg
      121 gcggcagata cagcatgtct ccgtgcccga gcagcgtctc tgcacccatc tgatcgagga
      181 tcgtgcgcga gtcgacccgc gccgacacct ggaaggcgat gcggcaggga atgttcgcct
      241 tgatgaggcc ggtgatcacg tccaccgacg gccgctgtgt cgcgagcacc aggtggattc
      301 ccgaggcgcg cgccttctgc gccaggcgcg tgatgagctc ctcgaccttc ttgccgacga
      361 tcatcatgag gtcggcaaac tcgtcgatga acactacgat gtaaggcagc ggcgtcagat
      421 cctcgatctg ccgctggtct atggtcggat cgttggcggc ccgcgccatc atcaccgggt
      481 cgcggatcgg cttgccggcg tcgattgcat ccttcacacg gcggttgaag ccggcgatgt
      541 tgcgcacccc gagcgccgcc atcagctggt gccgccggtc catctccacg accgaccagc
      601 gcagcgcgtt cgaggcctgc ttcatgtcgg tgaccaccgg cgccagcagg tgcgggatgc
      661 cctcgtagac cgacagctcg agcatcttcg ggtcgatcat gatgagccgc acgtgctcgg
      721 cggtcgactt ataaaggagc gacaacacca tggcgttgag cgccaccgac ttgccggaac
      781 ccgtgatgcc cgcgatcagc agatgcggca tgcggctcag gtccgcgacc accggctggc
      841 cgccgatgtc cttgccgagc gccagcgcca gcggcgagtg catctcgtcg taggcccgcg
      901 aacggatgat ctccccgagc gttacgacct cacgcttctc gttggcgatc tcgagcccca
      961 tgacgctctt gcccgggatc acttcgacga cccgcaccga cagcaccgag agcgcccgcg
     1021 ccaggtcctt ggaaagggcg gtgatctggc tcgccttgac cccgggcgcc ggccgcagct
     1081 cgaagcgcgt caccaccggc cccggctgca ccgccaccac ctcggcatcg atgccgaagt
     1141 ccttgagctt catctccacg aggcgcgaca gcgcctcgag cgcctcggcg gaatagagcg
     1201 gctcgcgcgc cggcggatcg tcgaggagtt tgagcggcgg cagctctttg gccttgggcg
     1261 gatcaaacag cggcacctgg cgctcgcgct cgacgcgtgc gctcttctcg accaccggca
     1321 gaggcggctc gatgcgcggt ggcgcacgcg tcgccgcctt cttctgctcg gtttcgaccg
     1381 cctccttgcg cgcctgacgc cgctcgcgtc cctcggccat gtccttgcgc gaggcccagc
     1441 gtgcccgcac ccagccgatg cccccccagg tgagcccgcc gagccggtcc atgacggcga
     1501 gccacgacac gtggaaggcg accgagatcc ccgccatcca ggcggcgatg aggagcagtg
     1561 tcgcgccaag gaagtcgagc caggccgcga gccccttgcc caccaggccg ccgaccactc
     1621 cgccggcggt ctggcgcagc gcccctgcct cccagtgcag cgtcgtcaga ccgcagctcg
     1681 ctgcgagcag cagcacgaag ccggccacgc gcaccacggt gttcacgcgc gagatactca
     1741 ggccccgcac gcggcgctgc agcccccacg cggcgatggc cagcaccacc ggcagcagga
     1801 acgccggcca tccgaacagg aaaaacagca cgtcggcgag ccaggcgccc gcgacgccga
     1861 tgcggttgtg gatcggcgca gcgttccccg tataagagaa tcccgggtcg tcggggctgt
     1921 agctggcgag cgccacgaac agcgtgagcg cgacgatcgc gagcgcgatc acggcgctct
     1981 cgcgcagggc gcgggagacg gccgccgtga ggcgcatgcc gttgcgtgcc tgaacctggg
     2041 tctcagccaa ccttcacttc cccatcttaa gatagacggc cagcattata gggcttggta
     2101 ccatgcaccg atggcgcaga tgacctcgac gaaaagtaag cacagccggt tgattatcct
     2161 gggatcggga cccgcaggtt acagcgccgc ggtgtacgcc gcgcgggcca atcgcgcgcc
     2221 gctcctcatc agcggaatgg cgccgggcgg ccagctgatg accaccaccg acgtcgacaa
     2281 ctggccgggc gacgtcgagg gcctgcaggg accggccctg atggagcgca tgcggcggca
     2341 cgccgagcgc ttcgcgaccg agatcgtgag cgatcatctg gtcgcggtgg atctcgcgac
     2401 gcgcccgttc agcctcactg gtgaggccga ccgctacagc tgcgacgcgc tcatcatcgc
     2461 cacgggcgcg agcgcccgct atctcggcct cccttcggag gagcgcttcc gcggccgcgg
     2521 tgtctcggcc tgtgccacct gcgacggctt cttcttccgc gaccagcgcg tggcggtggt
     2581 cggcggcggc aacaccgcgg tggaggaggc gctgtacctc gcgcacatcg cgcggcacgt
     2641 cacgctggtg catcggcgcg ataagctgcg tgccgagcgg atcctccagg accggctgtt
     2701 ccgcgaggtg caggccggca agatgtcggt ggtctggaac cacacggtgg acgaggtact
     2761 cggcaacgag cagggcgtga gtggtgtacg cctgcgtgcg ctgcccgccg gcgcgccgca
     2821 g
//
LOCUS       DATLKL010000503         2432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_96239, whole genome shotgun sequence.
ACCESSION   DATLKL010000503 DATLKL010000000
VERSION     DATLKL010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96239"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..2029)
                     /locus_tag="VM713_06730"
     CDS             complement(35..2029)
                     /locus_tag="VM713_06730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HVH83993.1"
                     /translation="MACTAAFGLAIGAAAVAPLSAAPEPSPAAPAEATQPATQMPPLL
                     PRREHGNLVFDNIPAPDPAFAARVARYLQSREATFLDWLPDGSLLVATRFAETEQVHR
                     IAAPLAAREQLTFYPEPVSFARAAESGGGFVFLKDKGGDENSQLYYRAADGAVRELTH
                     GDFIHGNPVWAHDGKRVAFYGNDRDGASYDVYVADVTSGAAPQLVVGGRQDAWYPLDW
                     SVDDSKLLVWRYVSISESYLYIADVASGTLTPLDTSRRKIGIRAAKFAPGGRGVYVLS
                     DEDGEYAALKLVDPVTHQTRRITPALGWDVEGFDASADGRYLAYVVNQDGRSRLVVQD
                     TQTHAEISPAGIPEGVITRIAFDRAAHRLGLSVESAASPRDVYSWDLEHERLERWTRS
                     EPGPLDRSALVAPELVHYPTWDRVAGQPRLLSAFVYRPRNAVHCPVLIDIHGGPESQF
                     RPGWDPFIQFLVNELGYAVIAPNVRGSAGYGKSFLALDNGELREDAVRDIGSLLVWIS
                     AQPAFDRDRISVMGASYGGYMTLASLVAYGQRLRGGIDVVGISNFVTFLTHTSAYRRD
                     LRRAEYGDDRDPHMRVFLERISPLTNASRIKRPLLVVEGLNDPRVPASESEQLVWRVR
                     AAGGEVWYLVAKDEGHGFRKKANRDAYLETAATFLQKLGR"
     gene            2132..>2432
                     /locus_tag="VM713_06735"
     CDS             2132..>2432
                     /locus_tag="VM713_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVH83994.1"
                     /translation="MPDAPRKPTIYRHPHGISAIDAEYLYSGHAAAHLILDSGRAAFV
                     DVGTNDSVPYLLAALAELGIAREAVDYVLLAHVHLDHAGGAGRLMRALPNARAVLH"
BASE COUNT          381 a          831 c          852 g          368 t
ORIGIN      
        1 gttcgcgcca gcctgaggcg cagggccgca gggcttaccg gccaagtttc tgcaaaaagg
       61 tcgcggcggt ctcgaggtag gcgtcgcggt tggccttctt gcgaaacccg tgaccctcat
      121 ccttcgccac caggtaccac acctccccgc ccgcggcgcg cacccgccag accagctgct
      181 cggactcgga cgccggcacg cgcggatcgt tcaggccctc gacgaccagc agcggccgct
      241 tgatgcggct cgcgttggtg agcggggaga tgcgctccag aaacacgcgc atgtgcggat
      301 cgcgatcgtc gccgtactcg gcgcggcgca ggtcgcgccg gtaggccgag gtgtgcgtga
      361 gaaacgtgac gaagttgctg atcccgacga cgtcgatgcc accgcgcagc cgctggccgt
      421 aggcgacgag cgaggcaagc gtcatgtagc cgccgtagga agcacccatg acggagatgc
      481 ggtcgcggtc gaaggccggc tgcgcgctga tccacaccag gagcgaaccg atgtcgcgca
      541 ccgcatcctc gcgcagctcg ccgttgtcga gcgcgaggaa gctcttgccg tagccggctg
      601 agccgcgcac gttgggcgcg atcacggcat agccgagctc gttgacgagg aactggatga
      661 acggatccca tccggggcgg aactgcgact ccggaccccc gtggatgtcg atgaggaccg
      721 gacagtggac ggcgttgcgt ggcctgtaca caaaggccga aagcagtcgc ggctggccgg
      781 ccacccggtc ccaggtgggg tagtggacga gctcgggggc taccagggcc gagcggtcga
      841 gcggccccgg ctcgctccgg gtccagcgct cgagccgctc gtgctcgagg tcccaggagt
      901 agacatcgcg cggcgatgcc gcggattcca ccgacaggcc gagccggtgc gcggcgcgat
      961 cgaaggcgat gcgcgtgatg accccctcgg ggatgccggc gggtgagatc tcggcatgcg
     1021 tttgcgtgtc ttgcacgacg agacggctac gcccgtcctg gttcaccacg taggcgaggt
     1081 agcgcccgtc ggcgctcgca tcgaacccct cgacgtccca gccgagcgcg ggcgtgatgc
     1141 ggcgcgtctg gtgcgtcacc ggatcgacca gcttgagtgc cgcgtattcg ccgtcctcgt
     1201 cggacagcac gtagaccccg cggccgccag gggcgaattt cgccgcgcga atgccgatct
     1261 tgcggcggct ggtatcgagc ggggtgagcg tgccgctcgc gacgtccgcg atgtagaggt
     1321 aactctcgct gatcgagacg tagcgccaca cgagaagctt gctgtcgtcc accgaccagt
     1381 cgagcggata ccaggcgtcc tgccgtccgc ccacgacgag ctgcggcgcg gcgccgctcg
     1441 tcacgtcggc gacatacacg tcgtagctcg cgccgtcacg gtcgttgcca tagaaggcga
     1501 cgcgcttgcc gtcgtgtgcc catacgggat tgccgtggat gaaatcgccg tgggtgagct
     1561 cgcgcacggc accgtcggcg gcccggtaat acagctgcga gttctcgtcg ccccccttgt
     1621 ccttgaggaa cacgaagcca ccgccgctct ccgccgcccg ggcgaagctg accggctcgg
     1681 ggtagaacgt gagctgctcg cgcgccgcca gtggggcggc aatcctgtgc acctgctcgg
     1741 tctccgcgaa gcgggtcgcg acgaggagcg acccgtccgg caaccagtcg aggaatgtcg
     1801 cctcgcgcga ctgcaggtag cgggcgaccc gcgcggcgaa cgcgggatcg ggcgcgggaa
     1861 tgttgtcgaa cacgagattg ccgtgctcgc ggcgcgggag gagcggcggc atctgcgtgg
     1921 ccggctgcgt cgcctccgcc ggcgcggctg gcgatggctc cggcgctgcc gacagcggtg
     1981 ccaccgccgc ggcgccgatg gccagcccga aggcggccgt gcacgccatc agagcacggc
     2041 tgccagcaat aacccgtatg gtcatgtgtg taagctagcg cacgagttac gatgtgtaca
     2101 cgcatcacct ttaaggcaag cctcaagccg cgtgcccgac gctccccgca agccgacgat
     2161 ctatcgccac ccgcacggca tcagtgcaat cgacgccgag tacctctatt ccggccatgc
     2221 ggccgcgcac ctgatcctgg attcgggccg cgcggccttc gtcgacgtcg gcaccaacga
     2281 ctcggtgccg tacctgctcg cggcgctcgc tgagctcggc atcgcccgtg aggcggtcga
     2341 ttacgtcctg ctggcgcacg tgcacctgga tcacgcgggc ggcgccgggc ggctgatgcg
     2401 cgccctcccc aacgcgcgtg ccgtgctgca tc
//
LOCUS       DATLKL010000504         1775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_96253, whole genome shotgun sequence.
ACCESSION   DATLKL010000504 DATLKL010000000
VERSION     DATLKL010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96253"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VM713_06740"
     CDS             complement(<1..1130)
                     /locus_tag="VM713_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVH83995.1"
                     /translation="MSTSAGTSAAKRDRGTWERLAAGVRPEGRAFIGGRLVPALDGAV
                     FDDVSPIDGQVIAQVARGRAADVDAAVKAARASFENGPWRRMEPKDRKKILRRFAELI
                     RADVDRLALLETRDVGKPIQNSVGVDVANCADCIEYYAEFSDKLYDEVAPTGPNDLAL
                     IRREPLGVVAAIVPWNYPLIISCWKVGPALLTGNSVVLKPAEQSPLSAIRLAQLALDA
                     GVPPGTFNVVPGYGEEAGQALALHRDVDLITFTGSTEVGRLMMRYAAESNLKRVALEL
                     GGKTPQVVFADADLEAAASGIAWGVYYNSGQTCHAGSRLIVHRAVRDPLLEALRRVTA
                     TITLGHPLESDTQMGALIDQGHMKRVLGYIEGGVADGARLALG"
     gene            complement(1213..>1775)
                     /locus_tag="VM713_06745"
     CDS             complement(1213..>1775)
                     /locus_tag="VM713_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003231438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HVH83996.1"
                     /translation="ALRVIDSAAATATLAAQVPDDHGVYLVPAFVGLGAPYWEPNARG
                     VLCGLTLGASAAHLARAALESVAYQTLDLTTAMERDGARRAEAFRVDGGMAANDWFCQ
                     FLADVLDARVERPVELETTALGAAFLAGLAAGVWKDLQSVAATWHKAAEFFPSMPAAR
                     RATLVAGWRDAIARTLLPAAAGPERR"
BASE COUNT          282 a          604 c          625 g          264 t
ORIGIN      
        1 cccagcgcaa gacgcgcgcc gtccgccacg ccgccttcga tgtagcccag cacccgcttc
       61 atgtgtccct gatcgatgag ggcacccatc tgcgtgtcgg actccagcgg gtgtccgaga
      121 gtgatggtgg cggtgacgcg gcgaagggct tcgagcagcg ggtcgcgcac cgcgcggtgc
      181 acgatcagcc gtgagccggc gtgacaggtt tgcccgctgt tgtagtagac cccccaggcg
      241 atgccggagg cggccgcctc gagatccgcg tccgcgaata ccacctgggg cgtcttgcca
      301 ccgagctcga gcgcgacgcg cttgagattc gattccgccg catagcgcat catcagccgc
      361 ccgacctcgg tcgagcccgt gaaggtgatg agatcgacat cgcggtggag ggcgagtgcc
      421 tggccggctt cctcgccata gcccggcacg acgttgaagg tcccgggcgg gacgccggcg
      481 tccagcgcga gctgcgccag acggatcgcg gagagcggcg attgctcggc gggcttcagc
      541 accacggagt tcccggtcag gagcgccggc ccgaccttcc agcagctgat gatgagcggg
      601 tagttccacg gcacgatcgc cgccacgacc ccgagcggct cgcggcggat cagcgccaga
      661 tcgttggggc cggtaggcgc cacttcatcg tagagcttgt cggagaactc cgcgtagtac
      721 tcgatgcagt cggcgcagtt cgcgacatcg acgccaaccg aattctgaat cggcttgccg
      781 acgtcgcgcg tttcgaggag tgcgagccga tcgacgtccg ctcggatcag ctccgcgaag
      841 cgccgcagga ttttcttgcg gtctttcggc tccatgcgcc gccacggacc gttctcgaag
      901 ctcgcacgcg cggccttgac cgccgcatcg acgtccgcgg cgcggccgcg cgccacctgg
      961 gcgatcacct gtccgtcgat cgggctcacg tcgtcgaaca cggcgccgtc gagggcgggg
     1021 accaggcggc cgccgatgaa ggcgcgcccc tcgggcctga cgccggccgc caggcgctcc
     1081 catgtgccgc gatcgcgctt tgctgctgag gtgcctgctg aggtgctcat gaggaattgg
     1141 ctctccagag gtaaggtttc acagcggcag gggccgtcaa gtctagaaaa ggaatagccc
     1201 catctaaaag agtcatcggc gctcaggacc ggccgcggcg gggagcagtg tgcgcgcgat
     1261 ggcgtcccgc cagcccgcga cgagggtcgc gcgccgcgcc gcgggcatcg aggggaagaa
     1321 ttccgccgct ttgtgccagg tcgccgcgac cgactggagg tccttccaga ctcccgcggc
     1381 aagtccggcg aggaaagcgg cccccagcgc cgtcgtctcg agctcgaccg gacgctcgac
     1441 ccgcgcgtcg agaacatcgg cgaggaactg gcagaaccag tcgttcgccg ccatgccccc
     1501 atcgaccctg aacgcctcag cgcggcgcgc accgtcgcgc tccattgcgg tcgtgagatc
     1561 gagcgtctgg taggcgaccg actcgagcgc ggcccgggcg agatgggcgg cggaggcacc
     1621 gagcgtcagc ccgcagagga cacctcgcgc atttggctcc cagtaaggcg cgccgaggcc
     1681 gacgaacgcg gggaccagat agacgccgtg gtcgtcggga acctgcgccg cgagggtggc
     1741 ggtcgcggcg gcgctgtcga tgactctcag ggcgt
//
LOCUS       DATLKL010000505         2229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_163768, whole genome shotgun sequence.
ACCESSION   DATLKL010000505 DATLKL010000000
VERSION     DATLKL010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163768"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1498
                     /locus_tag="VM713_06750"
     CDS             158..1498
                     /locus_tag="VM713_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVH83997.1"
                     /translation="MVDAWGERMPRSVGLLNAVAVSIGITIGSGIFRVPATVAGLLHD
                     PGSVILCWLLGGVMALCGALTFAELAASVPRSGGMFAYILEGFGPVPAFLFGWAELTV
                     VRAAALGAIATIFAEYLGYFVPLTAAEVREVAALAIVVVGVLNYLGVRRAAAVLSVTT
                     YAKYAAVVALGLLACTARGATTAHFTPLWSGAVSLSLLATALIPVMWTYDGWADVAAI
                     GGEISNPQQTLPRALIVGSASVALVYLLVNLAFIYVLPLPEMAGSRLIATTVATRIPL
                     LAGAGTGLIAGLVMLATFSGLNASLMVGARIFFAMGDRGLLFRAVARVSPRFDSPSVA
                     IWICTALGVVYVLQNDFAQLADKFILGIWPFYALSVAAVFVLRRRRPELPRPYRVWGY
                     PIVPLVFLLASAGMVANALITDPANTGVTLLIIAAGLPVYWLRARIAAGRRAFG"
     gene            1525..>2229
                     /locus_tag="VM713_06755"
     CDS             1525..>2229
                     /locus_tag="VM713_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH83998.1"
                     /translation="MSSRPAGKAAFVFVFIAVLVDSIGFGIILPVLPRLIMQLTGVTV
                     DRAAGYGGWLAFVYALLQFFCAPVLGNLSDRFGRRPVLLFALFALGIDYLIMGFAPAI
                     GWLFAGRAIAGVAGASFTPAYAYVADISPPEKRAQNFGLMGAAFGLGFILGPAIGGLL
                     GGLGPRAPFFTAGAIALANGTLGLIALPESLPQAARRPFEWRRANPLGTLRQILKYPT
                     VTWLLGALFLWQLGHQV"
BASE COUNT          262 a          797 c          745 g          425 t
ORIGIN      
        1 ccgagccatc aagaacgtcg tcgcctcatc ggtcgacctg cagtcggccg tcgcccagca
       61 gaacccgtcg gacgcagagc actgagcgaa cttccgcgcg ggcgacggcg cccatgcact
      121 agactcgcag gacctgcaag acgctgaggg gtcctcgatg gtcgacgcat ggggcgagcg
      181 catgccgcgc tcggtggggc tgctcaatgc cgtcgccgtg tcgatcggca tcacgatcgg
      241 cagcggcatc ttccgcgtcc ccgccactgt cgcgggtctc ctgcacgatc cggggtcggt
      301 aattctctgt tggctgctcg gtggcgtgat ggcactgtgc ggcgcactca ccttcgccga
      361 gcttgcggcc tcggtgccgc gctcgggcgg catgtttgcc tacatcctcg aaggcttcgg
      421 tccggtcccg gcgttcctgt tcggctgggc cgagctcacc gtggtgcggg cggcggcgct
      481 cggcgccatc gctaccatct ttgcggagta cctgggttat ttcgtgccgc tcacggccgc
      541 cgaggtgcgc gaggtcgcgg cgctcgccat cgtcgtcgtc ggcgtgctga actaccttgg
      601 cgtacggcgt gcggccgccg tgctcagcgt gacgacctac gccaagtacg cggcggtcgt
      661 ggcgctcggg ctcctcgcct gcaccgcgcg cggtgccacg acggcgcact tcaccccgct
      721 gtggagcgga gccgtgtcgc tgtcgctgct cgccaccgcg ctcattcccg tgatgtggac
      781 ctacgacggc tgggccgacg tggcggcgat cgggggcgag atcagcaatc cacagcagac
      841 cttgccgcgc gccctcatcg tcggcagcgc gagcgtcgcg ctcgtctacc tcctggtgaa
      901 cctcgccttc atctacgtgc tgccgttgcc ggagatggcg ggctcgaggc tgatcgctac
      961 gaccgtcgcg acgcgcatcc cgctgctcgc aggtgccggc accggattga tcgcggggct
     1021 ggtgatgctc gcgaccttca gcggtctcaa cgcctcgctc atggtgggcg cgcgcatctt
     1081 cttcgcgatg ggcgatcgcg gactgctgtt tcgcgccgta gcgcgcgtct cgccgcgctt
     1141 cgacagccca tcggtcgcga tctggatctg cactgcgctc ggcgtggtgt acgtgctgca
     1201 gaacgacttc gcgcagctcg ccgacaaatt catcctgggg atctggccct tctacgcgct
     1261 cagcgtcgcg gcggtcttcg tgctgcggcg gcggcgcccg gagctgccgc gcccctatcg
     1321 cgtgtggggc tatccgatcg tgcccctggt attcctgctc gcctcggccg gcatggtggc
     1381 gaacgcgctc atcaccgatc ccgccaacac tggcgtcacg ctgctcatca tcgctgcggg
     1441 gctgccggtg tactggctgc gcgcgcgcat cgcggccggg cgccgggcct tcggctaaga
     1501 tagcgcgacc ccacgaaagt ccccatgagc tcgcgcccag ccggcaaagc cgccttcgta
     1561 ttcgtcttta tcgccgtgct ggtggactcg atcgggttcg gcatcattct gcccgtgctg
     1621 ccgcggctca tcatgcagct gaccggcgtc accgtggacc gcgcggcggg ctacggcggc
     1681 tggctcgcct tcgtctatgc gctgctgcag ttcttctgcg caccggtgct gggaaacctc
     1741 agcgatcgct tcggccggcg gccggtgctg ctgttcgcgc tgtttgcgct cgggatcgat
     1801 tacctcatca tgggcttcgc gcccgccatc ggctggctgt tcgcgggccg cgccatcgca
     1861 ggggtcgcgg gcgcctcctt tacgcccgca tacgcctacg tcgccgacat cagcccgccg
     1921 gagaagcgcg ctcagaactt cggcctgatg ggcgcggcct tcgggctcgg cttcatcctg
     1981 ggtccggcca tcggcggcct gctcggcggc ttgggtccgc gcgcgccgtt cttcaccgcc
     2041 ggcgccatcg cactcgccaa cggtacgctg gggttaatcg cgctccccga gtccctgcca
     2101 caggccgcgc gccgcccgtt cgagtggcgc cgcgcgaatc cgctgggcac gctgcggcag
     2161 atcctcaagt acccgaccgt gacctggctg ctgggcgccc tgttcctgtg gcaactcggt
     2221 caccaggtg
//
LOCUS       DATLKL010000506         5359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_17006, whole genome shotgun sequence.
ACCESSION   DATLKL010000506 DATLKL010000000
VERSION     DATLKL010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17006"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1285
                     /locus_tag="VM713_06760"
     CDS             44..1285
                     /locus_tag="VM713_06760"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005193613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HVH83999.1"
                     /translation="MAGLGRAATEAAGTLALAPTAQKNAALEAAAAEIRARSGEILAA
                     NERDVAAARAAHMSGARLERLKLDAPRIEAIAASVATVVALPDPIGTTLTEWERPNGL
                     RIQRVRVPLGVIGIIYESRPNVTIDAGALCLKSGNAAILRGGSESRHSNATLHGCLAA
                     GLAAAQLPAACIQLVPTTDRAAVGYMLAGMSEYLDVIVPRGGKTLVARVQQEARVPVI
                     GHLEGNCHVYIDRDADVRMARTIALNAKMRRTGICGAAETLLVDSACVATHLTPIVGD
                     LLDAGCEVRGDAIVKRADPRVRPASEDDWYTEYLDAIIAARVVDGVDAAIAHIARYGS
                     AHTESIVTENQATAERFLQRVDSAIVLHNASTQFADGGEFGMGAEIGISTDRLHARGP
                     VGVEQLTSYKYVVRGAGQIRP"
     gene            complement(1309..1803)
                     /locus_tag="VM713_06765"
     CDS             complement(1309..1803)
                     /locus_tag="VM713_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HVH84000.1"
                     /translation="MPTAGAAVTASFRLVRTPIEVSALREALADPTCGGYAAFEGWVR
                     DHNEGQRVRRLEYEAYETLAVREAERIVAEAVARFGVKHAACAHRVGELAVGELAVWV
                     GVSAPHRDEAFRACRYIIDEIKHRLPIWKKEHYVNGDSGWVNCERCAAAPHEHGHGRP
                     GAQA"
     gene            complement(1808..2650)
                     /locus_tag="VM713_06770"
     CDS             complement(1808..2650)
                     /locus_tag="VM713_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HVH84001.1"
                     /translation="MSAPVYGLVLAGGRSSRMGQDKAALRVGDRSQLEQAMALLAPHV
                     ARAFVSVRADQRADPLRGRFEQIEDTRENLGPVAGIIAAQECFPQSAWLVLACDLPLL
                     DAATLEFLLRARAPERLATAFRSSHDRLPEPLCAVYEPASRAALAAYVARGQHCPRKF
                     LLQADVHLVDEPDPRALDNANTPEEYAQAMSTIAEAPAAATRRVTVQYFALLREQAGR
                     GTESLITAARTPRELYAELARRYPFSLDPAVLRVAINSEFGDWSAPLADGDAIVFIPP
                     VAGG"
     gene            complement(2647..3147)
                     /gene="moaC"
                     /locus_tag="VM713_06775"
     CDS             complement(2647..3147)
                     /gene="moaC"
                     /locus_tag="VM713_06775"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HVH84002.1"
                     /translation="MRRPRLSHLDARDRPAMVDVGAKEVTQRTAEAEARIRLPPAVAR
                     ALRASGHRTRKGPVFDTAILAGVMAAKRTHELIPFCHPLPLDHCQIEIEQCPAGVIMV
                     RCRVAVHHRTGVEMEALTGAAVAALTVYDMCKALSHDIEIAGLKLMAKAGGRRPFTRR
                     RARRAA"
     gene            complement(3147..4202)
                     /gene="moaA"
                     /locus_tag="VM713_06780"
     CDS             complement(3147..4202)
                     /gene="moaA"
                     /locus_tag="VM713_06780"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HVH84003.1"
                     /translation="MSAPEVIVPLTRRPAPRDTRARPMRDLRISVMDRCNFRCPYCMP
                     RETYHEKYRFLGSHERLSFDEIVRLARLFVQLGVRKVRLTGGEPLLRANLTDLIGDLT
                     TIPGVEDVALTTNGVLLGKYASELKAAGLKRITVSLDSLDPAVFARMSGGFGSPAEVL
                     DGIEHARRAGLEPIKINAVVQRGVNDHTVLELIERFRGTGVIVRFIEYMDVGNRNHWR
                     SELVVPSKELAARIAARWPLNSLEPTYRGEVAERYAFADGQGEVGFISSVSQPFCGDC
                     SRVRLSSDGVIYTCLFATHGTSLRDALRGGAGDDQLLELIADVWLRRADRYSELRASL
                     PHGAGEPRKVEMFYIGG"
     gene            complement(4279..>5359)
                     /locus_tag="VM713_06785"
     CDS             complement(4279..>5359)
                     /locus_tag="VM713_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HVH84004.1"
                     /translation="DQPPFDRVAMDGIALDRGAVAAGRRAFRVQATQAAGDPPLTLAA
                     ASACIEVMTGAVLPAGCDCVVPVEELTVRDGGALLAAAVRAEPWQNVHRRGSDTRQGT
                     LLVSAGTRLRAPEIAIAASAGMARIRVSSQPMLAVISTGNELIEPGEPVLAHQVRRSN
                     AYGIVSALRERGFQRVADDHIQDDAEELRERLRFHLETHDVLVLSGGVSMGRFDLVPQ
                     VLEELGVHAIFHKVAQRPGKPLLFAMAPSGAAVFGLPGNPVSTLVCLGRYVIPALFAS
                     LGQTPLPAERLALGAPVTVDPPLTHFLPVRIEQDDWGRPWAMPAPTNGSGDFTSLAGT
                     DGFVELPPGPNTYKKGFVTRLFRWS"
BASE COUNT          833 a         1872 c         1908 g          746 t
ORIGIN      
        1 gcccggctct ggtaccgtga acgcggatcg catcggcgag ctgatggcag ggctcggcag
       61 ggccgctacc gaagccgccg gtacgctcgc gctcgcgccc acggcgcaga agaacgcggc
      121 cctcgaggcg gcggcggccg agatccgcgc gcgcagtggc gagattctcg ccgccaatga
      181 gcgcgacgtg gcagcggcgc gggccgcgca catgagcggc gcacggctcg agcggctcaa
      241 gctcgatgcc ccgcgcatcg aggcaatcgc ggcaagcgtc gccacagtgg tcgcgctgcc
      301 cgatcccatc gggaccacgc tgacggaatg ggagcgcccc aacggactca ggatccagcg
      361 ggtgcgcgtg ccgctcggcg tcatcggcat catctacgag agccgcccca acgtgacgat
      421 cgacgcgggt gcgctgtgcc tgaaatccgg caatgcggcg atcctgcggg gcggctcgga
      481 aagccgccac tcgaatgcga cgctgcatgg ctgcctcgcg gcgggactcg ccgcggcgca
      541 gctccccgcc gcctgtatcc agctggtgcc gaccacggac cgcgccgcgg tcggttacat
      601 gctggccggc atgagcgagt acctggatgt catcgtgccg cgcggcggca agaccctggt
      661 cgcgcgcgtg cagcaggagg cgcgcgtgcc ggtgatcgga cacctggaag gcaactgcca
      721 cgtctatatc gatcgcgatg ccgatgttcg catggcacgc accatcgcgc tcaacgccaa
      781 gatgcgccgc acgggcatct gcggcgcggc cgagaccctc ctcgtcgata gcgcctgcgt
      841 tgcgacgcac ctcactccca tcgtgggcga cctgctggat gccggctgcg aggtgcgagg
      901 cgatgcgatc gtgaagaggg cggacccaag ggtgcggccg gcgagcgagg acgactggta
      961 cacggagtac ctcgacgcca tcatcgccgc gcgcgtcgtc gatggcgtcg atgccgccat
     1021 cgcccacatc gcccgttacg gctcggcgca caccgaatcg atcgtcacgg agaatcaggc
     1081 gacggccgag cgcttcctgc agcgggtcga cagcgcgatc gtgctgcaca acgcttcgac
     1141 ccagttcgcg gacggcggcg aattcggcat gggagccgag atcggcattt ccaccgaccg
     1201 cctgcacgca agaggtccgg tcggggtgga gcagctcacc agctacaagt acgtggtgcg
     1261 gggcgcgggc cagatccggc cctagtcatt gggcacatcc ctgtgccttc aggcttgggc
     1321 gccgggcctg ccgtggccgt gctcgtgcgg ggcggcggca caacgctcgc agttcaccca
     1381 gcctgaatcg ccgttgacat agtgctcttt tttccagatc ggcagccggt gcttgatctc
     1441 gtcgatgatg tagcggcagg cgcgaaaggc ctcgtcgcgg tgcggcgcgc tcacgcccac
     1501 ccacaccgcg agctcgccga cggcgagctc gccgacacga tgcgcgcagg cggcgtgctt
     1561 cacaccgaaa cgcgctacgg cctcggcgac gatgcgctcg gcttcgcgca ccgcgagcgt
     1621 ctcataagcc tcgtactcga ggcgccgcac gcgctgcccc tcattgtggt cgcgcaccca
     1681 gccttcaaac gccgcgtagc cgccgcaggt cgggtcggcg agcgcctcac gcagggcgct
     1741 cacctcgatc ggggtgcgca cgaggcgaaa cgacgcggtc acggcggcgc cagcggtcgg
     1801 catgggctca gccgcccgcg accggtggga tgaacacgat ggcatcgccg tcggcgagcg
     1861 gcgccgacca gtcgccgaac tcggagttga tggcgacgcg cagcacggcg ggatcgagcg
     1921 agaaggggta gcggcgcgcg agctcggcgt acagctcgcg cggcgtgcgc gcggccgtga
     1981 tgagcgactc cgtgccccgg ccggcctgct cgcgcagcag cgcgaagtac tgcaccgtca
     2041 cgcgccgggt cgcagcagcc ggcgcctctg cgatcgtgct catggcttga gcatattcct
     2101 cgggagtgtt ggcgttgtcg agcgcgcgcg ggtcgggctc gtcgacgagg tgcacatcgg
     2161 cctgcagcag gaacttgcgc ggacagtgct ggccgcgcgc cacgtaggcg gcgagagcgg
     2221 cgcgactggc gggctcgtat acggcgcaca acggctcggg cagccggtcg tggctcgagc
     2281 ggaacgcggt cgcgagccgc tcgggggcgc gcgcgcgcag cagaaactcg agcgtcgctg
     2341 catcgagcag cggcagatca cacgccagca ccagccaggc gctctgcggg aagcactcct
     2401 gcgcggcgat gatcccggcg acaggcccga ggttctcgcg cgtgtcctcg atctgctcga
     2461 agcgcccgcg cagcgggtcg gcgcgctgat cggcgcgcac cgagacgaag gcgcgcgcga
     2521 cgtgtggcgc gagcagcgcc atcgcctgct cgagctggct gcggtcgccc acgcgcagcg
     2581 ccgccttgtc ctggcccatg cggctgctgc gcccgccggc gagcaccagg ccgtagaccg
     2641 gcgcgctcat gcagcgcgcc gcgcgcgccg gcgcgtgaag ggacgccggc ccccggcttt
     2701 ggccatgagc ttgaggcccg caatctcgat gtcgtgggag agcgccttgc acatgtcgta
     2761 gacggtgagt gccgcgaccg cggcaccggt gagcgcctcc atctccactc cggtgcggtg
     2821 atgcaccgcg acgcggcaac gcaccatgat cacgcccgcg gggcattgct cgatctcgat
     2881 ttgacagtga tcgagcggca gcggatggca gaaggggatg agctcgtggg tgcgcttagc
     2941 cgccatgacg ccggcgagga tcgcggtatc gaacaccggc cccttgcggg tgcggtggcc
     3001 gctcgcgcgc agcgcccgcg ccaccgcggg cggcagacgg atgcgcgcct cggcctcagc
     3061 cgtccgctgc gtcacctcct tggcgccgac gtccaccatc gccgggcggt cgcgcgcgtc
     3121 gagatgcgag aggcgcgggc ggcgcatcag ccaccgatgt agaacatctc gaccttgcgc
     3181 ggctcgccgg cgccgtgcgg caggctggca cgcagctcgc tgtagcggtc ggcgcggcgc
     3241 agccacacgt ccgcgatgag ctcgagcagc tgatcgtcac ccgctccgcc gcgcagcgca
     3301 tcccgcaggc tcgtgccgtg agtggcgaac agacaggtat agatcacgcc gtcggaggag
     3361 aggcgcacac gcgagcaatc gccgcagaag ggttgcgaca ccgaggagat gaaaccaacc
     3421 tctccctggc cgtccgcaaa tgcgtagcgc tccgccacct cgccgcggta ggtaggctcg
     3481 agagaattca gcggccagcg tgccgcgatg cgtgccgcca gctccttcga cggcacgacc
     3541 agctcggagc gccagtgatt gcggttgccg acgtccatgt actcgatgaa gcgcacgatc
     3601 acgccggtac cgcgaaagcg ctcgatgagc tcgagcaccg tgtggtcgtt gacgccgcgc
     3661 tgcacgacag cgttgatctt gatcggctcg agtcccgcgc ggcgcgcgtg ctcgatgcca
     3721 tcgagcacct cggcgggact gccgaagccg ccgctcatgc gggcgaacac cgccgggtcg
     3781 aggctgtcga ggctcaccgt gatgcgcttc aggcccgccg ccttcagctc gctcgcgtac
     3841 ttcccgagca gcaccccgtt ggtcgtgagc gccacgtcct cgacgccggg gatcgtggtc
     3901 aggtcgccga tcaggtcggt caggttggcg cgcagcagcg gctcgcctcc ggtgaggcgg
     3961 actttccgta ccccgagctg cacgaacagg cgggcgaggc gcacgatctc atcgaacgac
     4021 agccgttcgt gcgagccgag aaaacgatac ttctcgtggt aggtctcgcg cggcatgcag
     4081 tacgggcaac ggaagttgca tcggtccatc accgagatgc gcagatcccg catcggccgc
     4141 gcgcgcgtgt cccgcggcgc gggcctgcgg gtgaggggaa cgatgacttc cggggcagac
     4201 attcctcaag tatgtgacgg tgcccgcgcg ctggctatcg gcgtaaagag ggaggtctgc
     4261 gcgacgactc cagcggcgtc acgaccagcg gaacagccgc gtgacgaagc ccttcttgta
     4321 ggtattcggg ccgggcggca gctccacgaa cccatcggtc cccgcaagcg acgtgaagtc
     4381 gcccgagccg ttggtgggag ccggcatcgc ccaggggcgc ccccagtcgt cctgctcgat
     4441 ccgtaccggc aggaagtgcg tgagcggcgg gtcgaccgtg accggcgcgc cgagcgccag
     4501 ccgttccgcc ggcagcggag tctgtccgag gctcgcgaac agcgcgggga tcacatagcg
     4561 gccgaggcac acgagcgtcg agacgggatt gccgggcaga ccgaacaccg cggcccccga
     4621 aggcgccatg gcgaacaaga ggggcttgcc ggggcgctgc gccaccttgt ggaagatcgc
     4681 gtgcacgccg agctcttcca acacctgcgg caccaggtcg aacctgccca tcgagacgcc
     4741 gcccgacagc acgagcacgt cgtgcgtctc gaggtggaat ctcaaccgct cgcgcagctc
     4801 ctcggcatcg tcctggatgt gatcgtccgc gacacgctgg aagccgcgct cgcgcagcgc
     4861 gctcacgatc ccgtaggcgt tggagcggcg cacctgatgg gcgagcaccg gctcgcccgg
     4921 ctcgatcagc tcgttgccgg tcgaaatgac cgcgagcatc ggctggctgc tgacgcggat
     4981 gcgcgccata ccggcggagg ccgcgatggc gatctccggc gcgcgcagcc gcgtgcccgc
     5041 cgacacgagc agcgtcccct ggcgcgtgtc gctgccacgc cggtgcacgt tttgccacgg
     5101 ttcggcgcgc accgcggcgg cgagcagcgc ccctccgtcc cgcaccgtga gctcctcgac
     5161 cggcacgacg cagtcgcagc ccgctgggag aactgcgccc gtcatgacct cgatgcaggc
     5221 gctggcggcg gcgagtgtga gcggcggatc gcccgcggcc tgtgtggcct gcacgcggaa
     5281 cgcgcgccgg ccggcggcca ccgcccccct gtcgagcgcg atgccgtcca tggccacgcg
     5341 gtcgaacggc ggctggtca
//
LOCUS       DATLKL010000507         1994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_107395, whole genome shotgun sequence.
ACCESSION   DATLKL010000507 DATLKL010000000
VERSION     DATLKL010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107395"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VM713_06790"
     CDS             complement(<1..876)
                     /locus_tag="VM713_06790"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVH84005.1"
                     /translation="MTMLEDNLAIERPTFVSHLECSQTGDRYPADQIHTLSRAGKPLL
                     VSYDLEGLSRAVSRTSLTKRACDLWRYREFLPVRRAENIVSLGETMTPLLSLPRLGRH
                     LGAGEVLMKDEGRLPTGSFKARGLCAAVSMAKELGIRRMAMATAGNAGAALAAYCSRA
                     GIESYVFCPEDAQDVNVGETVLQGARVYKVNGLINDCAKIVAEGKAARGWFDASTLKE
                     PYRIEGKKTMGLELAEQLGWTLPDAIFYPTGGGTGLIGMWKVFDELGKIGWIDSKRPR
                     MVAVQSTGCAPIVRAF"
     gene            1078..1935
                     /locus_tag="VM713_06795"
     CDS             1078..1935
                     /locus_tag="VM713_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVH84006.1"
                     /translation="MKTILLPLEESEALPSVLQTTLIAARIFGSYMEGLHVRPGLTGV
                     VAAGAEGLVAATPGLIESFEREDQARAQRVRTAFEEFMRRQGIPRSAAIAPANAPSWG
                     WFEEVGEGEAAIGKRGRVFDLIALARPKRGGSSAAMSTLETALFESGRPLLIAPPTSP
                     QELGGKIAVAWNGSTETARTVAFAMPFLARAREVLVLLVEGGTAPGPGAADAAQHLQR
                     NGIPAEPRVIKPGKRSIGETVLDEAARWGADLLIKGAYTQSRLRQMIFGGTTSHILAE
                     ATLPVFMAH"
BASE COUNT          328 a          693 c          636 g          337 t
ORIGIN      
        1 gaaggcgcgc acgatggggg cgcagcccgt tgactgcacc gcgaccatgc gcggccgctt
       61 gctgtcgatc caaccgattt tgcccaattc gtcgaacacc ttccacattc cgatgaggcc
      121 ggtaccgccg cccgtcggat agaagatcgc gtccggtagc gtccagccga gctgctcggc
      181 gagctccagg cccatggtct tcttgccctc gatgcggtat ggctccttga gcgtcgaggc
      241 gtcgaaccaa ccgcgcgccg ccttgccttc ggcaacgatc ttggcgcagt cgttgatgag
      301 tccattcacc ttgtagaccc tggctccctg cagaaccgtc tcgcccacat tcacgtcctg
      361 cgcgtcctcg ggacaaaaga cgtagctctc gatgcccgcc cggctgcagt acgcggccaa
      421 cgccgcgccc gcattgcctg cagtagccat cgccatccga cggatcccca gctccttcgc
      481 catcgagacg gcggcgcaca gcccgcgtgc cttgaacgat ccggtcggca gccggccctc
      541 gtccttcatc aagacttccc ctgcccctag atggcgcccg agccggggca agctgagcag
      601 gggcgtcatc gtctcgccga ggctcacgat gttctcagcg cgcctcaccg gcaggaactc
      661 gcgatagcgc cagaggtcgc aggcccgctt tgtcaggcta gtgcggctca cggcacggct
      721 gaggccctcg aggtcgtagg atacgagcag cggcttgcct gcgcgcgaaa gcgtgtgaat
      781 ctgatcggcc ggatagcgat cgccggtctg gctgcattcc aggtggctga caaatgtcgg
      841 gcgctcaatc gcgagattgt cttcgagcat ggtcatgcat ggcctccccg cgccccggga
      901 tcattcgcgc ggcgcgcggt tttcgtgacg gcttccttcg cagccctctt tcgccaagta
      961 ctacgcctcg ggcgcctcga tctagactgg attttcggtt cgcgcccaaa ccggcctgag
     1021 ttagaatggc cggccgcgcg aaggcttccg ccctgacctt atgagagagc gaagagcatg
     1081 aaaaccatcc tcctgcctct cgaggaaagc gaggccctcc cctccgtctt gcagaccacc
     1141 ctcatcgcgg cgcgcatttt cggcagctac atggaggggc ttcacgtacg gcctgggttg
     1201 accggcgtgg tcgcggccgg ggccgaaggg ctggtcgccg cgactcccgg cctcatcgaa
     1261 agctttgagc gcgaggatca ggcccgggcc cagcgggttc gcacagcctt cgaggaattc
     1321 atgcgccgac aaggaatccc gcgcagtgcc gccatcgcac ccgccaatgc gcccagctgg
     1381 ggctggttcg aggaggtggg agaaggcgag gcggcgatcg gcaaacgcgg gcgcgtgttc
     1441 gatctcatcg cgctggcccg acccaagcgc ggtggatcga gcgccgcgat gagcacgctc
     1501 gagaccgcgc tgttcgagag cggccggccg ctgctcatcg ccccacccac ctcgccccag
     1561 gagctcggcg ggaaaatcgc ggtggcgtgg aacgggtcga ccgaaaccgc gcgcaccgtc
     1621 gccttcgcca tgccgttcct ggcgcgtgca agggaggtgc tggttctgct cgtcgaaggc
     1681 ggcaccgccc ctgggccggg cgccgcggac gcggcccagc atctccagcg caacggcatc
     1741 ccggccgagc cgcgggtgat caagccgggc aagcgcagca taggcgaaac cgttctggat
     1801 gaggcggccc ggtggggcgc cgatctcctc atcaaaggtg cctatacgca aagccggctc
     1861 cggcagatga tcttcggcgg caccaccagc cacatccttg ccgaggcgac gctcccggtc
     1921 ttcatggcgc actgaacatc agttcctggc cgtcagcccg cgtcggtggc gacgtaacgt
     1981 acgtccgccg agag
//
LOCUS       DATLKL010000508         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_197788, whole genome shotgun sequence.
ACCESSION   DATLKL010000508 DATLKL010000000
VERSION     DATLKL010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197788"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /gene="ppsA"
                     /locus_tag="VM713_06800"
     CDS             complement(<1..1341)
                     /gene="ppsA"
                     /locus_tag="VM713_06800"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HVH84007.1"
                     /translation="MNAYVLPFDRLSLRDVESVGGKNASLGEMIGALGKLGVRVPGGF
                     ATTSHAYREFLAQGGLDARIRTELAGLDVDDVVKLAASGARIRQWVLATPFSAALTQA
                     VLAELARMSGGGEVAVAVRSSATAEDLPEASFAGQQESFLNVRGEAAVLEAMHQVFAS
                     LFNDRAISYRVHQGFDHGAVALSAGVQHMVRSDLGASGVMFTLDTDSGFRDVVFITAA
                     WGLGETVVQGAVNPDEFYLYKPALRAGKHAILRRNLGGKAVKMVYAPAASAARVATVE
                     VPEADRRRFCLSDADLETLARQALAIEEHYGRPMDIEWGKDGASGEIFILQARPETVQ
                     SRAGRTIQRYTLKSHSRVLAEGRSIGQRIGSGPARVIRDVSEIARVQSGDVLVADNTD
                     PDWEPVMKRAAAIVTNRGGRTCHAAIIARELGIPAVVGCVDATQHIHDGQPVTVS"
     gene            1432..>2064
                     /locus_tag="VM713_06805"
     CDS             1432..>2064
                     /locus_tag="VM713_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HVH84008.1"
                     /translation="MSRRTIFFVSDQTGVTAETMGHSLMTQFEGVEFRAVTLPFVSTI
                     DKAQEVVRRINRAAEDEGLRPIVFSTLVQDELRDIVAGANGLFLDFFAAFVGPLEREL
                     AMRSTHSAGRAHGIADLAAYTTRINATNFALANDDGTGGDYAHADVVLIGVSRVGKTP
                     TCVYMALQYGVFAANYPLTEDDLESGKLPARLEPYRAKLYALTIKPERLQQ"
BASE COUNT          313 a          724 c          698 g          329 t
ORIGIN      
        1 cgacacggtg accggctggc cgtcgtggat gtgctgcgtg gcgtcgacgc agccgacgac
       61 cgcgggaatc cccaactcgc gcgcgatgat cgccgcgtgg caggtgcggc cgccgcggtt
      121 ggtgacgatc gccgcggcgc gcttcatgac aggctcccag tcgggatcgg tgttgtccgc
      181 gaccagcacg tcgcccgact gcacgcgcgc gatctcgctc acgtcgcgga tgacgcgcgc
      241 ggggccggaa ccgatgcgct gtccgatgct gcggccctcg gcgagcacgc gcgagtggct
      301 cttgagggtg tagcgctgga tggtgcggcc ggcgcggctc tgcaccgtct cgggacgcgc
      361 ctgcagaatg aagatctcgc cgctggcacc gtccttgccc cactcgatgt ccatcggccg
      421 gccgtagtgc tcctcgatcg cgagcgcctg gcgcgccagc gtctcgaggt cggcgtcgct
      481 caggcagaag cgccgccggt cggcctccgg cacctcgacg gtcgcgacgc gcgcagcact
      541 cgcggccggt gcatacacca tcttgaccgc cttgccgccc aggttgcgcc gcagaatcgc
      601 gtgtttgccg gcgcgcagcg ccggcttgta gaggtagaac tcatcggggt tgaccgcgcc
      661 ctgcaccacg gtctcgccga gtccccacgc cgcggtgatg aataccacgt ccctgaagcc
      721 cgagtcggta tcgagggtga acatgacacc gctcgcgccg aggtcgctcc tcaccatgtg
      781 ctgcacgccg gcggagagtg cgaccgcgcc gtggtcgaag ccctggtgca cgcggtagga
      841 gatcgcgcgg tcgttgaaga gcgaggcgaa cacctggtgc atcgcctcga gcaccgcggc
      901 ctccccgcgc acgttgagaa agctctcctg ctgcccggcg aaggaggctt cgggcagatc
      961 ttcggcggtg gccgaggagc gcaccgcgac cgccacctcg cccccgccgc tcatgcgtgc
     1021 gagctccgcg agcaccgcct gggtgagcgc ggcgctgaag ggcgtggcga gcacccactg
     1081 gcggatgcgc gcgccgctcg cggcgagctt caccacgtcg tcgacgtcga ggcccgcgag
     1141 ctcggtgcgg atgcgcgcgt cgagtccacc ctgcgcgagg aactcgcgat aggcgtggct
     1201 cgtggtggcg aagccgcctg gcacgcgcac gccgagcttg ccgagggcgc cgatcatctc
     1261 cccgagcgag gcgttcttgc cgccgacgct ctcgacgtcg cgcagactca agcgatcgaa
     1321 gggcagcacg taggcgttca cactcgcaga tcctccggat ctcttgctcc gctgcgtgtg
     1381 cggtgaaaca cttgccgcgc gtcgcgccgc acgtgtcagc gcattcgagc tatgagccgt
     1441 cgaaccatct tcttcgtctc ggaccagacc ggcgtgaccg ccgaaacgat gggtcacagt
     1501 ctcatgaccc agttcgaggg ggtggaattt cgcgcggtga ctttgccatt tgtatcgacc
     1561 attgacaagg cgcaggaggt ggtccggcgc atcaaccgcg cggccgaaga cgagggcctg
     1621 cgcccgatcg tgttcagcac tctggtgcag gacgagttgc gcgacatcgt cgcgggcgcc
     1681 aacgggctgt tcctggattt cttcgctgcg ttcgtcggtc cgctcgagcg cgagctcgcc
     1741 atgcgctcca cgcacagtgc cgggcgcgcg catggcatcg ccgacctcgc cgcctacacc
     1801 acgcgcatca acgccaccaa cttcgcgctc gccaacgacg acggcaccgg cggcgactat
     1861 gcgcatgccg acgtggtgct gatcggcgta tcgcgtgtcg gcaagacccc gacctgcgtg
     1921 tacatggcgc tgcagtacgg agtcttcgcg gccaactacc cgctcaccga agacgatctg
     1981 gaaagtggca agctgcccgc gcggctcgag ccctatcgcg ccaagctcta cgcgctcacc
     2041 atcaagcctg agcggctgca gcag
//
LOCUS       DATLKL010000509         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_62392, whole genome shotgun sequence.
ACCESSION   DATLKL010000509 DATLKL010000000
VERSION     DATLKL010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62392"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VM713_06810"
     CDS             <1..192
                     /locus_tag="VM713_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84009.1"
                     /translation="GLSTRVRVEVSDSSGSRLATAPRREPVLATDAYDTDLSEIRARI
                     AEIIQDNSGKGRAAALARD"
     gene            complement(215..682)
                     /locus_tag="VM713_06815"
     CDS             complement(215..682)
                     /locus_tag="VM713_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVH84010.1"
                     /translation="MDKLDARILELLQTNGELTAADIADRVGLSKAPCWRRIQKLQQE
                     GVIRATVALLDAHALNVGTTVFVTLKTANHSEAWFAKFVRAVRDIPEVTEIHRMSGDV
                     DYLIRIVVPDIDAYDAVYKRLISAVDFLDVSASFALETIKYTTALPLCYLKTE"
     gene            795..1046
                     /locus_tag="VM713_06820"
     CDS             795..1046
                     /locus_tag="VM713_06820"
                     /inference="COORDINATES: protein motif:HMM:NF040386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6356 family protein"
                     /protein_id="HVH84011.1"
                     /translation="MNPIRAFTEHPASVGETYTEHMGRAVCFGLRMIGAGIACLVHAL
                     LPFLFVRTGSAAIAELNDRLVVNRRVTPPSLASTPRLPL"
     gene            1049..2182
                     /locus_tag="VM713_06825"
     CDS             1049..2182
                     /locus_tag="VM713_06825"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HVH84012.1"
                     /translation="MPGLLRALFPPWASLRQAAPGIAVAVALALVARAIADALAHGAA
                     GLPRLPLSPVMCAVVLGMLWRNTLGVPGWATNGLKWAMHQLLRTGIALVGLRLTLAGA
                     SSIALTALPVALTCLAVALFAGFALARLFGVGARLGALLAIGTAVCGCTAVVAMSPVI
                     RARHAETAFAVTCVVLFGCAAMLCYPWVAGHFFAPSPVHAGIFLGTAIHDTSQVIGAA
                     LMYSQQAAAPAALAAASVAKLLRNLSIAVLVPLAAWQARRHEARGGGAEHAADPHHAG
                     LAPHAQLVPLFVLAFLACIVARSAGDGLLGERSALWQAVVHTGYTASDLFLTCGMTAV
                     GLSVSFTDMWRIGWRPLAAGFMVATLTGVTSLTLTLTLLHFAH"
     gene            2286..>2585
                     /locus_tag="VM713_06830"
     CDS             2286..>2585
                     /locus_tag="VM713_06830"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HVH84013.1"
                     /translation="MARHRGKVSRSAAEEAVRTLMRWAGDDPAREGLRDTPQRVVDAY
                     RDWFSGYQIDPAAYLRRTFEEVAGYDEMIVLRDIAFESHCEHHIAPMIGRVHIGYV"
BASE COUNT          349 a          939 c          863 g          434 t
ORIGIN      
        1 ggactctcga cccgcgtgcg ggtcgaggtg agtgacagct ccggcagccg gctcgcgacc
       61 gcgccgcgtc gtgagccggt gctcgcaacg gacgcctacg acacggatct cagcgagatc
      121 cgcgcgcgca tcgccgagat catccaggac aactccggga aaggccgcgc ggctgcgctg
      181 gcgcgcgatt agcgcaggcg gcgcggcgcg cgcctcattc ggtcttcaga taacacagcg
      241 gcagcgcggt cgtgtacttg atcgtctcga gcgcgaagct ggcactcacg tcgaggaagt
      301 ccacggcaga gatgagccgc ttgtagaccg catcgtaagc gtcgatgtcc gggaccacga
      361 tgcggatcag gtagtcgacg tcacccgaca tgcggtgaat ctcggtgact tccgggatgt
      421 cgcgcaccgc gcgcacgaac ttggcgaacc acgcctcgct gtggttggcg gtcttgagcg
      481 tcacgaatac cgtggtgccg acattgaggg catgcgcgtc gagcagcgcc acggtcgccc
      541 ggataacccc ctcctgctgc agcttctgga tgcgacgcca gcacggcgcc ttggagagtc
      601 ccacgcggtc ggcaatgtcc gccgcggtca gctccccgtt ggtctggagc agctcgagga
      661 ttctggcgtc cagcttatcc attgaaactt cggtgcgtcc gaccccttca acgcgcaata
      721 ttgttgcgca cccggcccct ccgcgccaca gcttcgcaac cgcgtcgccc gccaccgctc
      781 ttagcatatt gaggatgaat cccatccggg ctttcaccga gcatcccgcc tcggtcggcg
      841 agacctatac cgagcacatg ggccgcgccg tctgcttcgg gctgcggatg atcggagcag
      901 gaatcgcctg cctggtgcac gcgctgctgc cgtttctgtt cgtgcgcacc ggaagcgcgg
      961 cgatcgccga gctgaacgat cgcctggtcg tcaatcggcg cgtcacgccg ccgtcgctcg
     1021 ccagcactcc gcgactgccg ctgtaatcgt gccgggcttg ctgcgggcgc tgtttccgcc
     1081 gtgggcctcg ctgcgccagg cggctccggg aatcgccgtc gccgtcgcgc tcgcgctcgt
     1141 ggcacgcgcc atcgccgatg cgctcgccca cggcgccgcg ggcctgccgc gcctgcctct
     1201 cagccccgtc atgtgcgcgg tggtcctcgg catgctctgg cgtaacaccc tcggcgtgcc
     1261 cggctgggcc accaacggtc tgaagtgggc catgcatcag ctgctgcgca ccggcatcgc
     1321 gctggtgggg ctgcgcctga cgctcgcggg cgcgagctcg attgccctga cggcactgcc
     1381 cgtggccctg acgtgcctcg ccgtcgcact cttcgccggc ttcgccctcg cgcgcctctt
     1441 cggagtcggc gcgcggctcg gagcgctgct ggcgatcggc acggcggtgt gcggctgcac
     1501 cgcggtggtg gcgatgtcgc ccgtgatacg cgcgcgccat gcggagaccg ccttcgccgt
     1561 cacctgcgtc gtgctgttcg gctgcgccgc catgctctgc tatccgtggg tggcggggca
     1621 tttcttcgcc ccctcgccag tgcacgccgg catctttctc ggcaccgcga tccacgacac
     1681 ctcgcaggtc atcggcgcag cgctcatgta ctcgcagcag gcagcggctc cggccgcgct
     1741 cgccgcggcg agtgtggcga agctcctgcg caacctctcg atcgccgtgc tggtgccgct
     1801 cgccgcctgg caggcgcgcc gccatgaggc gcgcggcggc ggcgccgagc acgccgccga
     1861 cccccaccac gccgggctcg cgccgcacgc gcagctcgtg ccgctgttcg tgttggcgtt
     1921 tctcgcctgc atcgtggcgc gcagcgccgg ggacgggctg ctcggggagc ggagcgcgct
     1981 ctggcaggcc gtcgtccaca ctggctacac cgcctccgac ctctttctca cctgcggcat
     2041 gaccgcagtg ggactctcgg tatccttcac cgacatgtgg cgcatcggct ggcggccgct
     2101 cgccgccggg ttcatggtgg cgaccctgac gggggtcacg agcctgacac tcacgctcac
     2161 tctgctgcac ttcgcgcact gacgcgcaca ccgcgacgcc gacactcccg gcgccgactt
     2221 ttccgacgcc gacacgctaa actgcccgga tccggcgccc cgcaggcgcc atcggggagg
     2281 gtggcatggc gaggcaccga gggaaggttt cgcgcagcgc ggccgaagag gcggtgcgca
     2341 cgctgatgcg ctgggcgggc gacgaccctg cgcgcgaggg gctgcgcgac acgccgcaac
     2401 gcgtcgtgga cgcttaccgc gactggttct ccggctatca gatcgacccc gccgcgtacc
     2461 tgcgccgcac cttcgaggaa gtcgcgggct acgacgagat gatcgtgttg cgcgacattg
     2521 ccttcgaatc acactgcgag caccacatcg ccccgatgat cgggcgcgtg cacatcggct
     2581 acgtg
//
LOCUS       DATLKL010000510         2285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_208984, whole genome shotgun sequence.
ACCESSION   DATLKL010000510 DATLKL010000000
VERSION     DATLKL010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208984"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /gene="pheT"
                     /locus_tag="VM713_06835"
     CDS             complement(<1..1578)
                     /gene="pheT"
                     /locus_tag="VM713_06835"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HVH84014.1"
                     /translation="MKVPYSWLTEWVKVPWQPRELGARLTMAGFELEGLETAAPAFTG
                     VVVAEILSAEPHPQAGKLKVCRVTIGTGSALKVVCGAPNARAGLKSALALVGAQLPGD
                     LKIGAAKLRGVESQGMLASAKELGLTDASSGILELPADAPLGKPLRDYLGLDEAVLDV
                     NITPNRGDAMSILGLAREVAAIAGTKVTGPRIESPRGAKRERFPVKLEAPAGCPRFAG
                     CIVRGVDNRAATPVWLTERLRRAGVRSISPVVDVTNYVMLELGQPMHAYDLAKLKEGI
                     RVRLARAGEEVTLLDGKTVKADPDMLLITDAEGPVGLAGIMGGQRTAVQPETTDVFFE
                     SAYFSPAAVQGRGRRLGLLTDASQRFERGVDPTQQARAVERALALLKPIAGGMAAGIV
                     VTENAKHLPKRSAVRLRAAQLARLLGLELPAARVSKALTSLGMKVSANATGWKATPPA
                     HRFDIEIEADLIEEVARLAAWERIPETDALGPQHFRKLPEEQPAERAVLEALAARGYE
                     EAVTYAFVDPALQEKLFP"
     gene            complement(1575..>2285)
                     /gene="pheS"
                     /locus_tag="VM713_06840"
     CDS             complement(1575..>2285)
                     /gene="pheS"
                     /locus_tag="VM713_06840"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004979194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HVH84015.1"
                     /translation="EIVAVFQRLGYSVAEGPVVETDYYNFEALNFPPNHPARDTQDTL
                     FIAGQQSKPQRERLLLRTHTSPVQIRALVEHGAPLAIIAPGRVYRIDSDMTHSPMFHQ
                     LEGLVVGEDVSFANMKAVLHGFLQAFFERDLALRLRPSYFPFTEPSAEVDMACLFCAG
                     AGCRTCKHTGWLEISGCGMVHPNVLKASGVDPERYTGYAFGAGIDRLAMLRYGVNDLR
                     LFFENDLRFLKQFRAFSS"
BASE COUNT          369 a          788 c          755 g          373 t
ORIGIN      
        1 cgggaagagt ttctcctgca gcgcgggatc cacgaatgcg taggtcacgg cttcctcgta
       61 accgcgcgcg gcgagcgcct cgagcaccgc gcgctcggcg ggctgctctt ccgggagctt
      121 gcggaaatgt tgtggcccga gtgcatcggt ctcgggaatc ctctcccagg cggcgagccg
      181 cgccacctct tcgatgaggt ccgcctcgat ctcaatatcg aaacggtgtg ccggtggagt
      241 ggccttccag cccgtcgcgt tcgctgacac cttcatgccg agactcgtga gcgctttgct
      301 gacgcgcgcg gcgggcagct cgaggccgag caggcgcgcg agctgcgcgg cgcggagcct
      361 caccgccgag cgtttcggga ggtgtttcgc attctcggtg accacgatgc ccgcggccat
      421 gccgccggcg atcggcttga gcagcgccag cgcgcgctcc accgcgcggg cctgctgcgt
      481 cgggtcaacc ccgcgctcga agcgttggct ggcgtcggtg aggagcccca agcgccgccc
      541 gcggccctgc actgccgcgg gcgagaagta agcgctctcg aaaaagacgt ccgtcgtctc
      601 aggctgcacc gcggtgcgct gtccccccat gatgccggcg agcccgacgg gcccctccgc
      661 atcggtgatg aggagcatgt cggggtcggc cttgaccgtc ttcccgtcga ggagcgtcac
      721 ctcctcgcca gcgcgtgcga gccgcacgcg gatgccttct ttgagcttcg cgaggtcgta
      781 ggcatgcatc ggctgcccga gctcgagcat gacgtaattg gtgacatcga cgaccggact
      841 gatcgagcgc acgccggccc tgcgcaagcg ctccgtcaac cacaccggag tcgcagcgcg
      901 gttgtcgacc ccgcgcacga tgcagccggc aaagcgaggg cagccggcag gcgcctcgag
      961 cttcaccgga aagcgctcgc gcttggctcc gcgcggactc tcgatcctcg gccccgtgac
     1021 ttttgttccc gcgatcgccg ccacctcacg cgctaacccc aagatcgaca tcgcatcgcc
     1081 gcgattcggc gtgatgttga cgtccagcac tgcctcatcg agcccgaggt aatcccgcag
     1141 cggcttgccg agcggcgcgt cggcgggaag ctcgagtatt ccgctcgagg catcggtgag
     1201 cccgagctcc ttggcggacg ccagcatccc ctgcgactcg acaccgcgga gcttcgcggc
     1261 gccgatcttg agatcgcccg ggagctgcgc gccgaccagt gccagtgcgc tcttcaggcc
     1321 cgcgcgtgca ttcggcgcac cgcacacgac cttgagggcc gagccggtcc cgatcgtcac
     1381 ccggcagacc tttagcttgc cagcctgagg gtgaggctcg gcggagagaa tctccgccac
     1441 cacgacgccg gtgaaggcgg gcgctgcggt ctcgagtccc tcgagctcaa agcccgccat
     1501 ggtgagacgc gcgccgagct cccgcggctg ccacggaacc ttcacccatt cagtgagcca
     1561 ggagtacggg accttcatga gctgaacgcg cggaattgct tgaggaaccg caggtcgttc
     1621 tcgaagaaca gccgcaggtc attcaccccg tagcgcagca tcgcgagccg atcaatcccg
     1681 gcaccgaagg cgtagccggt gtagcgctcg gggtcgacgc cgctcgcctt cagcacgttc
     1741 gggtgcacca tgccgcagcc cgagatctcc agccagccgg tgtgcttgca ggtgcggcag
     1801 ccggcacccg cgcagaacag gcacgccatg tcgacttcgg ccgagggctc ggtgaacggg
     1861 aaatacgagg gtctgaggcg cagcgccaga tcgcgctcga agaaggcctg caggaacccg
     1921 tgcagtaccg ccttcatgtt ggcgaagctt acgtcctccc ccaccaccag cccttccagc
     1981 tggtgaaaca tcggcgagtg cgtcatgtcc gagtcgatgc gatacacgcg tcccggcgcg
     2041 atgatcgcga gcggcgcgcc gtgctcgacc agggcgcgga tctgtaccgg cgaggtgtgc
     2101 gtgcgcaata gcaagcgctc ccgctgtggt ttcgactgct ggcccgcgat gaacagcgtg
     2161 tcctgcgtat cgcgcgccgg atgattcggc ggaaaattca gcgcctcgaa gttgtagtag
     2221 tcggtttcga cgaccggccc ttccgcgacg gagtagccga ggcgttggaa caccgcaacg
     2281 atttc
//
LOCUS       DATLKL010000511         2520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_51055, whole genome shotgun sequence.
ACCESSION   DATLKL010000511 DATLKL010000000
VERSION     DATLKL010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51055"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VM713_06845"
     CDS             <1..796
                     /locus_tag="VM713_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVH84016.1"
                     /translation="VLEPEVRVQADAARAALASGRTSAADALGGQALAASLEDRILAL
                     EQDQAMQQAELGHWIGADARRPLAGIPVNVDLHTTPEALLAEVPEHPPLAPVVAQLAE
                     AQTEVELARANERPDWSAEFDYAQRGPDFSNMVSLEFHVSLPLFGEHRQNPVIAEKLA
                     LVRAQEARRDEEVRMHTAELAGELAQWRLGRERLKRYADELLPLTRDRSRAAVASYSS
                     GRGDLRSAIDALTQEIDTQLVYVQLEGSVARAWAHLHWLHSVGSLP"
     gene            793..2067
                     /locus_tag="VM713_06850"
     CDS             793..2067
                     /locus_tag="VM713_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVH84017.1"
                     /translation="MSLARSLNPRGLGAVAVLIVGVGAGYWWGHRQAAPSLTPAAAGA
                     TQGEVLYWYDPMVPTQHFDKPGKSPFMDMQLVPKYAEAADQTNAVRVSPALEQNLGIR
                     LGRVERASLPARVRAVGSIAFDERLLELVQSRVDGYITELNVKAPLARVRRGQGLATV
                     LAPQWLEAQQEYLTLLDAQSQSAPALRAAARERLTVLGVPDAVVRSVEETRRANPYTV
                     IRAPIDGVVTELGAREGASFAAGTVLFRINGLTRVWANAQIPEARAAVAVAGSAVEAR
                     AAAWPGMVFKGQVLAVLPQVDSQTRTLTARVAIDNREFRLTPGMWVDVDFTEPAAAPQ
                     LFVPSEAVITTGRRSIVIVAKPGGSFDVAQVSTGLEEEGKTVILSGLTEGQSVVLSGQ
                     FLIDSEASLTATLERLTASGAPPPAQQGGTQP"
     gene            2064..>2520
                     /locus_tag="VM713_06855"
     CDS             2064..>2520
                     /locus_tag="VM713_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH84018.1"
                     /translation="MIERLIRWSIANRFLVMLATAFVAAWGVWALIGTPLDAIPDLSD
                     VQVIIRTTYPGQAPQIVENQVTYPLATTMLSVPGAKTVRGYSMFGDSFVYVLFEDGTD
                     LYWARSRVLEYLNQVQSRLPVDARTSLGPDATGVGWVYEYALIDKSGGHD"
BASE COUNT          414 a          813 c          864 g          429 t
ORIGIN      
        1 agtactcgag cccgaggtgc gggtgcaggc cgacgcggcg cgcgctgcgc tcgcaagcgg
       61 ccggacttcc gccgccgatg cgctgggggg gcaggcgctc gcggcctcgc tggaggaccg
      121 aattcttgcg ctcgagcagg accaggccat gcagcaggcc gagctcggac actggattgg
      181 cgcagacgct cggcggcccc tcgccgggat cccggtcaac gtagacctac atacgacacc
      241 cgaggcgctg ctcgctgagg tacccgaaca cccgccgctc gctccggtcg tggcgcagct
      301 cgcggaggcg cagaccgaag tcgagttggc acgagcgaac gagcgccccg attggagcgc
      361 ggagttcgac tacgcgcagc gcggcccgga cttctccaac atggtctcgc tcgaatttca
      421 cgtgagtctt ccgctctttg gcgagcaccg tcagaacccg gtgatcgccg agaaactcgc
      481 cttggtccgg gcccaggagg cccgacgtga cgaggaagtg cggatgcaca ccgcagagct
      541 cgcgggcgag ctcgcgcagt ggcgcctagg ccgtgagcga ctcaagcgtt acgccgacga
      601 gctcttgccg ctgacaagag accggtcccg tgcggcggtt gcctcgtata gctccggtcg
      661 tggtgatctc cgaagtgcga tcgacgccct cactcaagag atcgacaccc agcttgtgta
      721 tgtccagctc gaaggttcgg tagcccgcgc ctgggcgcac cttcactggc tgcacagcgt
      781 cgggagcctg ccatgagcct cgctcgatcg ctgaacccgc ggggactggg agcggtcgcc
      841 gtgctcatcg tgggcgtcgg cgccggctat tggtggggac acaggcaagc agctccatcg
      901 ctgacgcccg ccgcggccgg ggcgactcag ggcgaggtgc tgtactggta tgaccccatg
      961 gtccccacac aacacttcga caagcccggg aagtctccct tcatggacat gcagctcgtg
     1021 ccgaagtacg cggaggccgc tgaccagaca aacgcggtgc gcgtgagtcc ggccctcgag
     1081 cagaacctgg gaatccgcct tggaagggtc gagcgcgcat cgctcccggc ccgcgtgcgg
     1141 gcggtgggaa gcattgcgtt cgatgaacga ctgctcgagc tcgtgcagtc tcgcgtcgac
     1201 ggttacatca ccgagctcaa cgtgaaggcc cctctggctc gtgtgcggcg cgggcaaggc
     1261 ctcgcgaccg tactcgcacc gcagtggctc gaagctcaac aggagtacct cacgcttctc
     1321 gatgcgcaat cgcaaagcgc gcccgcgctg cgagctgcgg ctcgcgagcg tctgaccgtg
     1381 ttgggggtgc cggatgcggt agtgcgcagc gtcgaggaaa cacgccgggc gaatccgtac
     1441 accgtcatcc gcgcgccaat cgacggagtg gtgaccgagc tcggggcgcg ggaaggcgcg
     1501 agtttcgcgg ccggcacggt gctctttcgg atcaatggac tcacacgcgt gtgggccaac
     1561 gcccagatcc ccgaggcgcg agctgccgtc gcggtggcag ggtctgccgt cgaggctcgc
     1621 gccgccgcct ggccgggcat ggtgtttaag ggtcaggtgc tcgctgtgct gccgcaggtg
     1681 gactcccaga cccgcacgct cacggcgcgc gtggcgatcg acaaccggga gttccgactg
     1741 acgccgggga tgtgggtcga cgtggatttc actgagccag ccgccgcgcc gcagctgttt
     1801 gttcccagcg aggcggtcat cacgactggc aggcgcagca ttgtcatcgt ggcgaagccg
     1861 ggcggttcat tcgatgtggc acaggtctcg accggtctcg aagaggaggg caagacggta
     1921 atcctctccg gcctcaccga aggtcagtcg gtggtcctat cgggccaatt tctgatcgac
     1981 tcagaggcaa gcctgaccgc caccctcgag cggctgactg cgagcggcgc gccgcctccc
     2041 gcacagcagg gcgggacgca gccatgatcg agcggctcat ccgctggtcg attgccaatc
     2101 gtttcctcgt gatgctggcg acggcctttg tcgctgcgtg gggtgtgtgg gcgctcatag
     2161 gcaccccact cgatgcgatt ccggaccttt cggacgtgca ggtcatcatc cgcacgacct
     2221 accccggaca ggcaccgcag atcgttgaga atcaggtcac ctatccgctc gcgaccacga
     2281 tgctgtcggt accgggtgcg aagacggtgc gcggctactc gatgtttggg gactcttttg
     2341 tctacgtgct gtttgaagat ggcacggacc tgtattgggc gcgatcgcgc gtgctggagt
     2401 acctgaatca ggtgcagtcg cgattgcccg tcgatgcccg gacgtcgctt ggtccggatg
     2461 ccaccggtgt cggctgggtg tacgagtacg ctctcatcga caagagcggc ggccatgacc
//
LOCUS       DATLKL010000512         2955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_175058, whole genome shotgun sequence.
ACCESSION   DATLKL010000512 DATLKL010000000
VERSION     DATLKL010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175058"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VM713_06860"
     CDS             complement(<1..536)
                     /locus_tag="VM713_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HVH84019.1"
                     /translation="MKYFLVMPAAGSGRRFQGDIPKQYAPLVGRTVIEWALAPFLGDP
                     RCAHAVVVIAEADATWHRIAARLTDERLSATPGGAERSVSVRRGLAALAGRAGPDDWV
                     LVHDAARPCLDVRDLDELLRRVSLHGTGGLLAVRATDTLKQARPGPQGSGLAGAATVD
                     RTDLWRALTPQMFRFGAL"
     gene            complement(596..910)
                     /gene="ftsB"
                     /locus_tag="VM713_06865"
     CDS             complement(596..910)
                     /gene="ftsB"
                     /locus_tag="VM713_06865"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HVH84020.1"
                     /translation="MKWLAAALAVVLVVLQYRIWVSDSGVRQVQRLRQAVAAQQAEDG
                     QLAERNRQLAAEVRDLKTGMDALEERARSDLGMIARNETFYQVVPAHPPAAPPPTRTA
                     AR"
     gene            complement(954..2246)
                     /gene="eno"
                     /locus_tag="VM713_06870"
     CDS             complement(954..2246)
                     /gene="eno"
                     /locus_tag="VM713_06870"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HVH84021.1"
                     /translation="MSRISEVRGREILDSRGNPTVEVDVLLDSGALGRAAVPSGASTG
                     TREAVELRDGDKRRYGGKGVLRAVEHVNTVLKTAVVGRDPRDQAAIDAGMIELDGTEN
                     KGRLGANAILGVSLAAARAAAADARLPLYRYLAQLSPRRHEPVMPVPQMNIVNGGAHA
                     NNSLDIQEFMILPVGAPSFREALRYGAEVFHTLKKLLADRGLSTAVGDEGGFAPSLES
                     NEAALALIVEAIEKAGYKAGRDIQLGLDVASSEFFRDGRYQLESEQRSFTAAEFARYL
                     AGLAARYPIVTIEDGMSEADWDGWAELTRVLGPQLQLVGDDVFVTNTKILREAIDKRI
                     ANAILIKPNQIGTLTETLACIDMADAARYAAVISHRSGETEDSTIADIAVATPATQIK
                     TGSLSRSDRIAKYNQLLRIEAELGTVRFAGRDAFPARH"
     gene            complement(2243..>2955)
                     /gene="kdsA"
                     /locus_tag="VM713_06875"
     CDS             complement(2243..>2955)
                     /gene="kdsA"
                     /locus_tag="VM713_06875"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:TIGR01362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HVH84022.1"
                     /translation="RFAVPFIFKASYDKANRSSRASFRGPGMDAGLKVLEHVRNRIGV
                     PVLTDVHEDTPLAEVAAVVDVLQTPAFLCRQTNFIVNAASQGKPVNIKKGQFLSPWEM
                     QNVVDKARSTGNDSILVCERGFSFGYNNLVSDMRSLSILRATGCPVVFDATHSVQLPG
                     GQGTSSGGQREFIPVLARAAVAAGVAGLFMETHPRPEEALSDGPNAWPLARMEPLLAT
                     LTELDRAVKAAPFEESLL"
BASE COUNT          453 a          995 c         1043 g          464 t
ORIGIN      
        1 cacaaggcgc cgaaccggaa catctgcggc gtgagggccc gccataggtc ggtccgatcc
       61 accgtcgcgg cgcccgccag gcctgagcct tgaggcccgg ggcgcgcctg cttcagggta
      121 tccgtggcgc gcaccgcaag caggccacct gtgccgtgca ggctcactcg cctcaagagt
      181 tcgtccaggt cgcgcacgtc gaggcacggg cgcgctgcat cgtgcaccag cacccagtcg
      241 tcggggccgg cgcggccggc gagcgccgcg agcccgcggc gcaccgacac gctgcgctca
      301 gcgcccccgg gcgtcgccga gaggcgctca tccgtcaggc gcgcggcgat gcggtgccag
      361 gtggcgtcgg cttcggcgat cacgactacg gcgtgcgcgc agcgcggatc gccgagaaaa
      421 ggcgccaagg cccattcgat gacggtccgc cccacaagcg gcgcgtactg tttcggtata
      481 tcgccctgga agcgccggcc ggagcccgcc gccggcatga cgagaaaata tttcattggc
      541 tgggagggcg cctcagcctc atttgaccac ggttgcgctg gagcggcagc gctcgtcagc
      601 gagccgcggt gcgggttggc ggaggcgcgg cgggcgggtg ggccggcacc acctggtaga
      661 aggtctcgtt gcgggcaatc atgccgaggt cgctgcgggc gcgttcttcg agcgcgtcca
      721 tgccggtctt caaatcacgt acttcggcgg cgagctggcg gttgcgctcg gccagctggc
      781 catcctcggc ctgctgcgca gcgaccgcct gccgcaggcg ctgcacctga cgcacgccgc
      841 tgtcggaaac ccagatccgg tattgcagca cgacgagaac gacagccagc gcggcggcga
      901 gccacttcat gggaaggaca gcgtaacaga cggcgccggg tacgcgaagg gcatcagtgc
      961 cgcgccggga aggcgtcccg gccggcgaag cgcacagtgc cgagctccgc ttcgatgcgc
     1021 agcagctggt tgtacttggc gatgcggtcg gagcgcgaca gcgagccggt cttgatctgt
     1081 gtggcggggg ttgccaccgc gatgtcggca atggtgctgt cctcggtctc accggagcgg
     1141 tgggagatca ccgcggcata gcgggccgcg tcggccatgt cgatgcaggc gagcgtctcg
     1201 gtgagcgtgc cgatctggtt gggcttgatg aggatcgcgt tggcgatgcg cttatcgatg
     1261 gcctcgcgca ggatcttcgt gttggtgacg aacacgtcat cccccaccag ctgcagctgc
     1321 gggccgagca cacgggtgag ctcggcccac ccgtcccagt cggcctcgct catgccgtcc
     1381 tcgatggtga cgatcgggta cctggcggcg agcccggcga gatagcgcgc gaattcggct
     1441 gcggtgaaac tgcgctgctc cgactcgagc tggtaacgcc cgtcacggaa gaactccgag
     1501 ctcgccacgt cgagcccgag ctggatgtcg cgccccgcct tgtagccggc cttctcgatc
     1561 gcctcgacga tcagggcgag ggccgcctcg ttcgactcga ggctcggcgc gaagccgccc
     1621 tcgtcgccga ccgcggtgga caggccgcgg tcggccagca gcttcttcaa cgtatggaag
     1681 acttcagcgc cgtagcgcag cgcctcgcgg aagctcggcg cacccaccgg caggatcatg
     1741 aactcctgaa tgtcgagact gttgttggcg tgcgcaccgc cgttcacgat gttcatctgc
     1801 ggcaccggca tcaccggctc gtgccgccgg ggtgagagct gtgcgaggta gcgatagagc
     1861 ggcagccgtg catcggccgc cgccgcgcgg gccgccgcca gtgacacgcc gaggatggcg
     1921 ttcgcgccga gccgaccctt gttctcggtg ccgtcgagct cgatcatgcc cgcatcgatc
     1981 gcggcctggt cgcgcgggtc gcgtcccacg accgcagtct tgagtaccgt gttgacgtgc
     2041 tcgacggccc tgagcactcc cttgccgccg tagcgccgct tatcgccgtc gcgcagctcc
     2101 actgcttcgc gcgtcccggt ggacgcaccc gaaggcaccg cggcgcgccc gagcgcgccc
     2161 gaatcgagca gcacgtcgac ttcgaccgtc ggattgccgc gtgaatccag gatctcgcgg
     2221 ccgcggactt cgctgatacg gctcatagga gtgactcctc gaacggtgcc gccttgaccg
     2281 cgcgatcgag ctcggtgagc gtcgcgagca gcggctccat gcgcgcgagc ggccaggcat
     2341 tggggccatc ggagagcgct tcttcgggcc gcgggtgcgt ctccatgaag agccccgcga
     2401 cgcccgcggc taccgccgcc cgcgccagga ccgggatgaa ctcacgctgt ccgccggagg
     2461 aggtcccctg cccgcccggc agctgcaccg agtgagtagc gtcgaagact accgggcagc
     2521 cggttgcgcg caggatcgac agcgagcgca tgtccgagac cagattgttg tagccgaagg
     2581 agaacccgcg ctcgcacacc agaatcgagt cgttgccggt ggaacgtgcc ttgtcgacca
     2641 cgttctgcat ctcccacggc gagagaaact ggcccttctt gatgttgacg ggcttgccct
     2701 gggaggcggc attgacgatg aagttggtct ggcggcagag gaacgcggga gtctgcagca
     2761 cgtccaccac ggcggcgact tccgcgagcg gggtgtcctc gtgcacgtcg gtgagcactg
     2821 ggacgccgat acggttcctg acgtgctcga ggaccttgag cccggcgtcc attcccggtc
     2881 cgcggaacga ggcccgcgag gagcggttgg ccttgtcgta ggacgccttg aagatgaacg
     2941 gcaccgcgaa gcggc
//
LOCUS       DATLKL010000513         1556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_175063, whole genome shotgun sequence.
ACCESSION   DATLKL010000513 DATLKL010000000
VERSION     DATLKL010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175063"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VM713_06880"
     CDS             <1..406
                     /locus_tag="VM713_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84023.1"
                     /translation="NPLHLEAGIASVHAAARSFEATDWQQLGRYYETLLELKPTPVVR
                     LNAAIVRAYAVDAAAGLAELEALASEKKLAHYAPYHAARGELLVKLGRDAEAAAALER
                     ALECPVNRAEREYLTNKLRSCAAEPGTRAALR"
     gene            540..1508
                     /locus_tag="VM713_06885"
     CDS             540..1508
                     /locus_tag="VM713_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVH84024.1"
                     /translation="MNRHSLTRRQFTARCAAFGLSVPALSGILAAPAGAQAPATGAAS
                     KPSPRSLKIPDGTAVTTLGQGCWHLGQGRHPVAVEEEALRTGISLGMTLLDTSGNYGR
                     GRSEKFLSHVIADQRDRIFLVSKVEGDEVSGDGIARACAASLARLGTSYLDLYLLHWP
                     IPSARFPGVVAGFEHLRAAGRIRAWGVSNFDVGQMEDLFRVPDGNRCATNQVPYSLNN
                     RHIEADVLPWCRQHNMPVMAYSPLGGDRHLVVGERTLAQIGATHGCPAAAVALAWVIR
                     GGNVIAIPESGSPEHVRENAAALSAALTPQDLETLNAAFPGPSGAS"
BASE COUNT          252 a          539 c          523 g          242 t
ORIGIN      
        1 gaacccgctg cacctcgaag ccggcattgc ctcggtgcac gccgcggcgc gctccttcga
       61 ggcgaccgac tggcagcagc tcggacgcta ctacgagacg ctgctggaac tgaagccgac
      121 gccggtggtg cggctcaacg ccgcgatcgt cagggcctat gcggtggatg ctgccgccgg
      181 acttgccgag ctcgaggcgc tcgcgagcga gaagaagctg gcacactacg cgccctatca
      241 cgctgcgcgc ggcgagctcc tcgtgaagct cgggcgcgac gccgaagccg ccgccgcgct
      301 cgagcgcgcg ctcgaatgtc cggtgaatcg cgccgagcgg gagtacctga cgaacaagct
      361 gcgcagctgc gccgcagagc ctggcacgcg cgccgcactc cgctagcgct tcaggaatcc
      421 gcgcgcaagc tgtggccgat tcgcggccac gccgatgtga atggcccttg gtctcaggga
      481 ggggtacaag gacggtctag ccgcgccggg gccatgctcc ctgcaaagga ggcggacaga
      541 tgaacaggca ctcactgaca cggcgtcaat tcaccgcgcg ctgtgccgcc tttggcttat
      601 cggtccctgc gctcagcggc atactcgcgg cgccggcagg agcgcaggcc cccgccaccg
      661 gcgcggcatc gaagccttct ccgcgcagcc tgaagatccc ggacggcact gccgtaacga
      721 cactcggcca gggctgctgg cacctcggtc agggaagaca cccggtcgcc gtcgaggaag
      781 aggcgctgcg caccggcatc tcgctcggca tgacgctgct cgacacctcc ggaaactacg
      841 gccgggggcg ctcggagaaa ttcctcagtc acgtgattgc cgatcagcgc gaccgcatat
      901 tcctcgtctc caaggtcgag ggcgatgagg tgtcgggcga tggcatcgcg cgggcgtgcg
      961 ctgcgagcct cgcccggctc ggcaccagct acctcgatct ctatctgctg cactggccca
     1021 ttcccagcgc ccggtttccg ggcgtcgtcg ccggattcga gcacctgcgc gcagcgggca
     1081 ggattcgcgc ctggggcgta tccaacttcg acgtcggcca gatggaggat ctgttccgcg
     1141 ttccggacgg caatcggtgc gcgaccaacc aggtgcctta cagtctcaat aaccgccaca
     1201 tcgaggcgga cgttctcccg tggtgcaggc agcacaacat gccggtgatg gcgtattcgc
     1261 cgctcggtgg cgaccggcac ctcgtggtcg gggagcgcac gctggcgcag atcggcgcca
     1321 ctcacggctg cccggccgcc gcggtggcgc tcgcctgggt catccgcggc ggcaacgtca
     1381 ttgcgattcc cgagtccggc tctccggagc acgtcaggga gaacgcggcg gcgctgtcgg
     1441 cggcactcac gccgcaggac ctcgagacac tgaatgccgc ctttccgggg ccgtccggcg
     1501 cgagctagtc ctgtcatcag cgaaaccgat gtggtccaat ctcgtacttt gaggtg
//
LOCUS       DATLKL010000514         1562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_62442, whole genome shotgun sequence.
ACCESSION   DATLKL010000514 DATLKL010000000
VERSION     DATLKL010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62442"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VM713_06890"
     CDS             complement(<1..1012)
                     /locus_tag="VM713_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH84025.1"
                     /translation="MNESALRLPGVHAAGRTAVTVLAAISFCHLLNDMMQSLLPALYP
                     MLKSRYQMSFGQIGLLTFTFQFTASLLQPMVGAMADRTPRPYSLAIGMGFSLAGLLLL
                     AFAGSYPLLLLAAALIGTGSSVFHPESSRVARMASGGKHGLARSVFQVGGNLGSACGP
                     LLAAFVVLPYGHSSVAWFSLVALLGMFVLFNVGHWYKAHGITRLAHAARTRAIEASPR
                     RVAGAIAVLLALIFSKYFYLASITSYYTFYLIGHFQQSVRSAQLHLFLFLAAAAIGGF
                     VGGPIGDRIGSKKIIWVSILGVLPFTLLLPHANLFWTSVLTVPIGLIIASAFPAIVVY
                     AQD"
     gene            1168..>1562
                     /locus_tag="VM713_06895"
     CDS             1168..>1562
                     /locus_tag="VM713_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVH84026.1"
                     /translation="MKDLTQGPITGQLVAMAVPIAIGMLFQTLYLVVDMYFVAHLGDA
                     AIAGVSTAGTAMFVIMALTQMLGVGTVALVSHAVGARDQAQANLVFNQSVTLSAICGA
                     ATLAAGWLLGGMYVRSMAADAATAAAGSA"
BASE COUNT          282 a          489 c          565 g          226 t
ORIGIN      
        1 ggtcctgcgc gtacaccacg atcgccggga aggccgaggc gatgatgagt ccgattggca
       61 cggtcaatac gctggtccag aagagattcg cgtgcggcag cagcagcgtg aagggcagca
      121 cgcccaggat cgacacccag atgatctttt tactgccgat gcggtcgccg atcggcccgc
      181 cgacgaagcc gccgatcgcg gccgccgcca ggaacaggaa caggtgcagc tgcgcgctgc
      241 gcaccgactg ctgaaagtgg ccgatgagat agaaggtgta atagctcgtg atgctggcga
      301 ggtagaagta cttggagaag atcagcgcca gcagcaccgc gatcgcgccc gcgacgcggc
      361 gcggcgaggc ctcgatggcg cgggtgcgcg ccgcgtgcgc gagtcgcgtg atgccgtgcg
      421 ccttgtacca gtggccgacg ttgaacagca cgaacatgcc gagaagcgcc accagcgaga
      481 accaggcgac gctcgagtgc ccgtagggca gcacgacgaa ggccgccagc agcggtccgc
      541 acgccgagcc gaggttgccg ccgacctgga acaccgatcg cgcgaggccg tgtttgccgc
      601 ccgaggccat gcgcgccacg cgcgaggact cgggatgaaa caccgacgag ccggtgccga
      661 tgagggccgc tgccagcagc agcagcggat agctgccggc gaaggcgagc agcagcagtc
      721 ccgcgagcga gaaccccatg ccgatggcga gcgagtaggg ccgcggagtg cggtcggcca
      781 tcgcgccgac catcggctgc aggagcgagg ccgtgaactg gaaggtgaag gtgaggagcc
      841 cgatctgccc gaagctcatc tgatagcggc tcttcagcat cgggtacagc gccggcagca
      901 gcgactgcat catgtcgttc agcagatggc agaagctgat cgccgcgagg acggtgacgg
      961 cggtgcggcc cgcggcgtgg acgccgggca ggcgcagggc ggattcgttc atgggggcgc
     1021 aattgtccac ggattgtcga tgggggccaa tctccctgaa tgggcgggcg gcaacccgcc
     1081 cgtgatctgc ggtggcagcc gcgcaatccc gtggcaagtg cgccccgcct catctaatgt
     1141 ggcgtccctt tggtgcgggg gagcctcatg aaagacctga cccagggccc gataaccgga
     1201 caactggtcg cgatggcggt gccgatcgcc atcggcatgc tatttcagac cctgtacctg
     1261 gtggtcgaca tgtacttcgt ggcgcacctc ggcgatgcag ccatcgccgg ggtcagtacc
     1321 gccggtaccg ccatgttcgt catcatggcc ctcacgcaga tgctgggggt gggcaccgtc
     1381 gcgctggtct cacacgccgt gggagccagg gaccaggcac aggcgaacct cgttttcaat
     1441 cagtcggtga cgctgtcggc gatctgcggt gcggcgaccc tcgccgccgg gtggctgctc
     1501 ggcggcatgt acgtgcgctc gatggcggcg gatgcggcga ccgcggccgc gggcagcgcc
     1561 tt
//
LOCUS       DATLKL010000515         2329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_28615, whole genome shotgun sequence.
ACCESSION   DATLKL010000515 DATLKL010000000
VERSION     DATLKL010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28615"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..700
                     /locus_tag="VM713_06900"
     CDS             68..700
                     /locus_tag="VM713_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVH84027.1"
                     /translation="MASVLIADDHAMVRAGLRRWLEKDPAIDKIGEATSGTDTLERLR
                     DGAWDVVVLDINMPDRSGIDVLAHIRSGYPKTRVLVISAFSEKQYAIYALRAGAAGYL
                     AKDQAPEDFMRAIHAVLAGRRFVSAALAEMLVGALDEPADQPLHATLSQREFQILCKL
                     AVGRSVSEIARELCISVKTVSTYRARILEKMKFSTNAELTAYALKSGLML"
     gene            765..1151
                     /locus_tag="VM713_06905"
     CDS             765..1151
                     /locus_tag="VM713_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH84028.1"
                     /translation="MRVLLVEDSSLLAGRITELIRRLPRVDLIGTVATEGDALVQVAA
                     AAPDVIILDLHLRDGSGFGVLRALERADARRPKVIVLTGFDLPQYRREAESLGVDAFL
                     NKSRDYHRLPVLLSALAAHDGSRTRR"
     gene            complement(1152..2210)
                     /locus_tag="VM713_06910"
     CDS             complement(1152..2210)
                     /locus_tag="VM713_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVH84029.1"
                     /translation="MAQQGEVRRDAQTIEVFGTQGRAAALQIIRTDAGATTMSQIDDL
                     VRKIAALDGAEALESARRWQISRWTGIATTSAALVTSAGLLLLLSRLTLRQRRSQEQE
                     TEQLTVRQGELERLVELRTEELSELSTHLQSVAEQEKAQLSRELHDELGGLLVAARMD
                     LSWLEEHVASDDPEVRGYFQRVHEALQAGVDIKRRVIESLRPTLLDNLGLVPALRWQV
                     ADSCERAGLRHVEHYPEDDLRLTPEASIAVFRIVQEALANAVKHARATAVEVTLEVVE
                     PWLLVRVRDDGVGVPPERLLALGAHGIAAMRQRARGLGGHGHVQRPAAGGTQIEVRLP
                     LARVLAREGAESRRRRGA"
BASE COUNT          364 a          829 c          759 g          377 t
ORIGIN      
        1 cctacacaga gtcaggcaga tggcgtactc tcaactccat cgcaggttta gggggcaagc
       61 gtactgcatg gcgagcgtgc tcattgccga cgatcacgcg atggtgcggg ccggcctgcg
      121 gcgctggctc gaaaaggacc cggcgatcga caagatcggt gaggccacga gcggcaccga
      181 caccctcgag cggctacgcg acggcgcgtg ggacgtggtg gtgctcgaca tcaacatgcc
      241 ggaccgcagc ggtatcgacg tcctcgcgca catccgcagc gggtatccga agacgcgcgt
      301 cctggtgatc agtgcgttct ccgagaagca gtatgcgatc tacgccttgc gcgcgggggc
      361 cgccggctac ctcgccaagg atcaggcccc cgaggatttc atgcgcgcga ttcatgccgt
      421 gctcgccggc cggcgcttcg tgagcgccgc gcttgccgag atgctggtcg gcgcactcga
      481 tgagccggcg gatcagccgc tgcacgcgac gctctcgcaa cgcgaattcc agatcctgtg
      541 caagctggcc gtgggccgca gcgtctcgga gatcgcccgg gagctgtgca tcagcgtcaa
      601 gaccgtgagc acttaccgcg cacgcatcct ggagaaaatg aaattcagca ccaatgccga
      661 gcttacggcc tacgccctga agagcggcct gatgctgtag agcctcatca ggtcgtctga
      721 caggctcatt gccgcgaccg cctgatatcg cgaacatcag ccccgtgcgc gtgctgctcg
      781 tcgaggactc cagtttgctg gccggtcgca tcacggagct gatccgtcgc ctcccgcggg
      841 tagacctgat cggcaccgtc gcaaccgagg gcgacgccct cgtgcaggta gcggccgcgg
      901 ccccggacgt aatcattctc gatctgcatc tgcgcgacgg ctcgggcttc ggcgtgctgc
      961 gcgcgctcga gcgcgcggac gcacgacggc ccaaggtcat cgtcctcacg ggcttcgacc
     1021 tgccgcagta tcggcgcgag gccgagtcgc tcggcgtcga tgccttcctc aataaatccc
     1081 gcgattatca ccggctgccg gtcctgctga gcgcccttgc cgctcacgat ggcagccgaa
     1141 cccggcgctg atcaggcgcc gcgccgacgc cgactctccg ctccttcccg ggccagcacg
     1201 cgcgcgagtg gcaggcgcac ctcgatctgg gtaccgcctg cagccgggcg ctgcacgtgc
     1261 ccgtggccgc cgagtccccg cgcccgctgc cgcatggccg cgataccgtg cgccccgagc
     1321 gccagcagcc gctcgggcgg cactcccacg ccgtcgtcgc gcacgcgcac gagcagccag
     1381 ggctcgacaa cttccagcgt cacctcgacc gccgttgcgc gcgcatgttt gacggcgttt
     1441 gcgagcgcct cctgcacgat cctgaagacc gcaatcgacg cctcgggtgt gagtcgcaga
     1501 tcgtcctccg ggtagtgctc gacgtgccgg aggccggcgc gctcgcagga atcggccacc
     1561 tgccagcgca gcgccggcac cagtccgagg ttgtcgagca gcgtcgggcg caaactctcg
     1621 atcacgcgcc gcttgatgtc cacgcctgcc tgcagcgcct cgtgcacacg ctggaagtag
     1681 ccgcgcacct ccggatcgtc gctggcgacg tgttcctcga gccatgacag atccatgcgc
     1741 gccgcgacca gaagtcctcc gagctcatcg tgcagctcgc gcgatagctg agccttctcc
     1801 tgctcggcca ccgactgcag atgcgtcgac agctccgaca gctcctcggt gcgcagctcg
     1861 accagccgct cgagctcgcc ctggcgcacc gtcagctgct cggtctcctg ctcctgggag
     1921 cgccgctgcc gcagcgtgag gcgactcagc agcagcagca gtcccgcgct cgtcacgagt
     1981 gctgcgctgg ttgtcgcgat gcccgtccag cggctgatct gccagcggcg ggcggactcg
     2041 agcgcttcgg cgccatccag cgccgcgatc ttgcgcacca gatcgtcgat ctgcgacatg
     2101 gtggtggcgc cggcgtcggt gcggatgatc tgcagggccg cggcgcggcc ctgcgtcccg
     2161 aagacctcga tggtctgcgc atctcgtcga acttcgcctt gctgagccac cgcagctgca
     2221 taacgttgga acgcgtagcc gagcccgcag ccgaataagc ggcgtcgagg cgctgcagcg
     2281 cgccgtcaag gtccgaggag gcctgccgga agggcctgag atactcggt
//
LOCUS       DATLKL010000516         1846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_175099, whole genome shotgun sequence.
ACCESSION   DATLKL010000516 DATLKL010000000
VERSION     DATLKL010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175099"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VM713_06915"
     CDS             <1..205
                     /locus_tag="VM713_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH84030.1"
                     /translation="SELAPPGDLAFAERLLTEGGVATIPLSPFYAAPPALSCLRLCIA
                     KRDSTLDEAAARLAAFAARLGKG"
     gene            209..994
                     /locus_tag="VM713_06920"
     CDS             209..994
                     /locus_tag="VM713_06920"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HVH84031.1"
                     /translation="MAALRVTLVQQPLVWGEPAANRERFAALLTPLAGSTDLVVLPET
                     FTTGFSMEVERLGEPAGGPTGVWLQDLSGELDAAITGSVITADGGRYYNRLLWAEPGG
                     TPLKHYDKRHLFRMGREHQHFTPGASAWSVPWRGFRICPLVCYDLRFPVFGRRRAALD
                     YELLLYVANWPAPRALAWRTLLRARAIENQAYVVGVNRVGPDGHGIAHAGDSAAIDYL
                     GQPLAELGGEPALVTVELDAARLHAFREKFPAHLDADRFTLEP"
     gene            1004..>1846
                     /locus_tag="VM713_06925"
     CDS             1004..>1846
                     /locus_tag="VM713_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH84032.1"
                     /translation="MKKPTRVNHPPEVTLPPGNRAVVAPIYQSVKFEFDTLEETERFL
                     RGERPGFFYTRTSNPTTRQLEQLLAELQGREDCLVTATGVGAIAQTLLALTRAGDHIL
                     CFVETYNPTRYLIRRVLGRFGVTHTMLSIEDRAGIERVLATQPVRLVYFESPTNPVTK
                     IADIAAITHAARAARALTVMDNTFAGFHQHGEFDVDVFLHSLTKYASGAGDVMGGAVI
                     ARAELIRTMHTDFGALGGTLDPHAAFLILRALKSYFVRYQAQCERALEIAKLLEHHPA
                     VARVH"
BASE COUNT          278 a          677 c          592 g          299 t
ORIGIN      
        1 cagcgagctc gctccgccgg gcgatctcgc cttcgccgag cggctgctca ccgaaggtgg
       61 ggtggcaacc attccgctgt cgcccttcta tgcagcgccc ccggcgctca gctgcctgcg
      121 cctgtgcatc gccaaacgcg acagcacact cgatgaggcc gcagcaaggc tcgcggcgtt
      181 cgcggcgcgc ctcggtaagg gctgagtcat ggccgcactg cgcgtcactc tggtccagca
      241 gcccctcgtg tggggcgagc cggccgccaa ccgcgagcgt ttcgccgccc tgctgacgcc
      301 gctcgccggc agcaccgatc tcgtcgtgct gcccgagacc ttcacgaccg gtttcagcat
      361 ggaggtcgag cgcctcggcg agccggccgg ggggccgacg ggcgtgtggc ttcaggatct
      421 ctcgggcgag ctcgacgcgg cgatcaccgg gagcgttatc acggcggatg gcggccgcta
      481 ctacaaccgg ctgctgtggg ccgagcccgg cggcacgcca ctcaagcact acgacaagcg
      541 gcacctgttt cgcatgggac gggagcatca gcacttcacc cccggagcct cggcctggag
      601 cgtgccgtgg cggggctttc gcatctgtcc gctcgtgtgc tacgacctgc gcttcccggt
      661 cttcggccgc cgccgcgccg cgctcgacta cgagctgctg ctctatgtcg cgaactggcc
      721 cgcgccacgc gcgctcgcct ggcggacgct gctgcgcgcg cgcgcgattg agaaccaggc
      781 ctacgtggtc ggcgtcaatc gcgtgggccc cgacgggcat gggatcgcgc acgcaggtga
      841 cagcgccgcg atcgattacc tcgggcagcc gctcgccgag ctcggcggcg agccggcgct
      901 cgtcacggtc gagctcgacg ccgcgcgcct ccacgccttc agggagaaat tcccggccca
      961 tctcgatgcc gaccgcttca cgctcgagcc ctgacccacg ccgatgaaaa agcccactcg
     1021 tgtcaaccat ccccccgagg tcactcttcc tccgggcaac cgcgcggtgg tggcgcccat
     1081 ctaccagagc gtcaagttcg agttcgacac tctcgaggag accgagcgct tcctgcgcgg
     1141 cgagcgtccg ggattcttct atacgcgcac ctcgaacccc acgacccgtc agctcgagca
     1201 gctgctcgcc gagctccagg ggcgcgagga ctgcctggtc acggcgaccg gtgtcggcgc
     1261 cattgcccag acgctgctcg cgctaacccg agcgggcgat cacatcctgt gctttgtcga
     1321 gacgtacaat ccgacccgct atctcattcg ccgcgtactc ggccgtttcg gtgtgacgca
     1381 taccatgctg tcgatcgagg accgcgctgg gatcgagcgg gtgctcgcca cccagccggt
     1441 gcgcctggtg tacttcgaga gccccacgaa tcccgtgacc aagatcgccg acatcgccgc
     1501 gatcacgcac gcggcacgtg ccgctcgcgc gctcacggta atggacaaca ccttcgccgg
     1561 atttcaccag cacggcgagt tcgacgtgga cgtatttctc cacagcctca ccaagtacgc
     1621 ctcgggcgcc ggggacgtca tgggaggggc ggtgattgcg cgcgccgagc tcatccgcac
     1681 catgcacacc gacttcggcg cgctcggcgg gacgctcgat ccgcacgccg ccttcctgat
     1741 cctgcgggca ctcaagagct acttcgtgcg ctaccaggcg cagtgcgagc gcgcgctcga
     1801 gatcgcgaag ctcctcgagc atcatccggc ggtggcacgg gtgcat
//
LOCUS       DATLKL010000517         1501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_220265, whole genome shotgun sequence.
ACCESSION   DATLKL010000517 DATLKL010000000
VERSION     DATLKL010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220265"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..1292
                     /locus_tag="VM713_06930"
     CDS             330..1292
                     /locus_tag="VM713_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84033.1"
                     /translation="MAERAESVIPAEADIDRAPGLLDGASSLELTPQTCNLEYWLGAV
                     AQGTLRGLVNGGHRAETATPAWMREPGPLRQALIEEFAFRSIAEEKATRALSYLVLHA
                     PGIDTMEFFATQLIDEARHSSVFRGHLLELGVAQDELQATIEQVAGEDREKVLVPLEN
                     FGLPLGRDAGDFIGGVCVLTVLVEGVLAPTSELSELKWRLLDPAAAEIERAAGIDEIR
                     HLTVGSAVIQQYLQANPGEVPRLKDLIERGRALWGSLPVLDMILRREQLFQEGMQQLA
                     HVIGDYELAPGRRLIDTTPEERLMMAATWSMEMQDSRLSAMGLG"
     gene            1306..>1501
                     /locus_tag="VM713_06935"
     CDS             1306..>1501
                     /locus_tag="VM713_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84034.1"
                     /translation="MERLTTALLAHRRLVLAAWVLLIALGGVFAVGLPGRIVPGGEAP
                     ASSQSGVVARELAHSPLPSLF"
BASE COUNT          241 a          502 c          527 g          231 t
ORIGIN      
        1 cgctggccag cgcgcagcgc agcggcgtcc acaccgactc actcgccgcg taaccgtgta
       61 ccagcagcac ggggtagcgg gcgttgggcc cggcgacata ggagatgctc tgcgaccccc
      121 gtagccgcag cgcgcgcgga aggttgaccg ccgccatccc tacggctcgc atttcctgca
      181 gcatcagcat gcctacgaag agggcgacag acggggcgtg atacagcgaa tcgattcgta
      241 tcagaccggg ctacggcggc tccatgccga atgagtaaac gcggccccat atctgtggga
      301 tgagccgctc atgaagattg gtgagcacca tggctgaacg agcagaatcc gtcatccccg
      361 ctgaggccga catcgaccgg gcacccgggt tgctcgacgg agcgagctcc ctcgagctga
      421 ccccgcagac gtgcaacctt gaatactggc tcggtgccgt cgcccagggc acgctgcgcg
      481 ggctggtgaa cggcggtcat cgcgccgaga cggcgacgcc ggcctggatg cgcgagccgg
      541 gcccgctgcg ccaggcgctg atcgaggagt ttgcgttccg ctcgattgcc gaggagaagg
      601 ccacccgcgc gctgtcctat ctcgtgctgc acgcacccgg catcgacacc atggagttct
      661 tcgcgacgca gctcatcgac gaggcgcggc actcctcggt ctttcgcggc cacctgctag
      721 agctgggcgt ggcgcaggac gagctgcaag ccaccatcga gcaggtcgct ggcgaggatc
      781 gtgagaaggt cctcgtcccg ctcgagaact tcgggcttcc gctcggacgc gacgcgggcg
      841 acttcatcgg cggcgtctgc gtcctcaccg tacttgtcga aggggtactg gccccgacgt
      901 cggagctgag cgagctcaag tggcgactgc tcgatccggc agcggccgag atcgagcgag
      961 ccgcgggcat cgacgagatc cgtcacctga ccgtcggcag cgcggtcatc cagcagtacc
     1021 tgcaggcgaa cccgggtgag gtgccccggc tgaaggacct gatcgagcgc ggccgcgccc
     1081 tgtggggcag cctgccggtg ctggacatga tcctccggcg cgagcagctg ttccaggaag
     1141 gcatgcagca gctcgcgcac gtcatcggcg actatgagct cgcgcccggc cggcgcctga
     1201 tcgacaccac cccggaggag cggctgatga tggccgccac ctggtcgatg gagatgcagg
     1261 acagccggct gtcggccatg ggcctgggct gatcggggaa cgacgatgga gcgcctgacg
     1321 accgccctcc tggctcaccg acggctggtg ctggctgcgt gggtactgct gatcgcgctg
     1381 ggcggcgtgt tcgccgtggg cctccccggc cgcattgtcc cgggcgggga agccccggcg
     1441 tcaagccagt ccggtgtggt ggcgcgcgag ctggcgcaca gcccgctgcc gtcgttgttc
     1501 g
//
LOCUS       DATLKL010000518         3842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_163939, whole genome shotgun sequence.
ACCESSION   DATLKL010000518 DATLKL010000000
VERSION     DATLKL010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163939"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..698
                     /gene="rpiA"
                     /locus_tag="VM713_06940"
     CDS             39..698
                     /gene="rpiA"
                     /locus_tag="VM713_06940"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HVH84035.1"
                     /translation="MSADADTKKRRAAAAALELVADESIIGIGTGSTVNLFIDALARL
                     PHRPRRAVSSSQASTTRLAAIGIEVRDLNEVSELNLYFDGADEATPARQLIKGGGGAL
                     TREKIIAAAARRFVCIVDDSKVVETLGRFPLPIEVIPMARTLVSRELTARGARPLWRE
                     GFVTDNGNHILDVHGLRITDPAALERELNLIPGVVTVGLFAVRPADVLIVGTARGVET
                     R"
     gene            complement(702..1286)
                     /locus_tag="VM713_06945"
     CDS             complement(702..1286)
                     /locus_tag="VM713_06945"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HVH84036.1"
                     /translation="MATESTISLPPLPYEHQALEPYMSRETLDYHYGKHHKAYVDNVN
                     MLIRGTPQQQATLEELVMRTDGALFDNAAQAWNHGFFWRCLSPRRQAPGRELEGALRS
                     FGGLDALEREFSRAALAVFGSGWAWLIKDRSGAVRIVTTANAHTPLRDGHTPLLACDV
                     WEHAYYVDHRNDRGAYLRSFWQLVNWDFVASNLH"
     gene            complement(1440..1513)
                     /locus_tag="VM713_06950"
     tRNA            complement(1440..1513)
                     /locus_tag="VM713_06950"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1479..1481),aa:Gln,seq:ctg)
     gene            complement(1531..1926)
                     /gene="rpsI"
                     /locus_tag="VM713_06955"
     CDS             complement(1531..1926)
                     /gene="rpsI"
                     /locus_tag="VM713_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HVH84037.1"
                     /translation="MPATPNHYGTGRRKTATARVYLKPGSGKITVNDRSLDEFFGRET
                     GRMIVRQPLDAVKLHTKFDISVQVDGGGITGQAGAIRHGITRALIDYDESLRKPLRDA
                     GFVTRDAREVERKKVGRRKARRGTQYSKR"
     gene            complement(1937..2368)
                     /gene="rplM"
                     /locus_tag="VM713_06960"
     CDS             complement(1937..2368)
                     /gene="rplM"
                     /locus_tag="VM713_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HVH84038.1"
                     /translation="MKTVFAKPASVRGDWFIVDASDKTLGRLASQIAHRLKGKHKADY
                     SPHVDMGDHIVVVNAQKIRVTGRKLTDKIYYHHTGHIGGIKSISLGKLLAEHPERAIE
                     FAVKGMLPKNPLGRRVFRKLHVFAGGEHPHQAQQPKPLEIQ"
     gene            2559..3197
                     /gene="coq7"
                     /locus_tag="VM713_06965"
     CDS             2559..3197
                     /gene="coq7"
                     /locus_tag="VM713_06965"
                     /inference="COORDINATES: protein motif:HMM:NF033656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HVH84039.1"
                     /translation="MESGRSLDAAIAAVDRALRSVFAPARAARPLPGTPQKEAPPLTA
                     AQQRESAALMRVNHSGELAAQALYHGQALVARSEAIRALLLEAARSEADHLAWCETRL
                     AELNARPSLLNPLWYVGSFAIGALTALAGDRTSLGFVVETERQVEGHLDEHLDRLPVG
                     DSRSRAILETMRAEEIAHGATAAAAGGAPLPAPVRALMRQTARVMTRTAYWV"
     gene            3242..>3842
                     /gene="crp"
                     /locus_tag="VM713_06970"
     CDS             3242..>3842
                     /gene="crp"
                     /locus_tag="VM713_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016506431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HVH84040.1"
                     /translation="MEENIKPLSEIPAINRFLSYCRIRAVPSKTVVIHAGDLPDVLYY
                     IISGSVEVMIEDEEGNEMVLAYLNRGQFFGEMGLFYEQPTRSAWVRTRNQCELAEMTY
                     PRFRQIAAESPGLVFELATQLATRLDRTNRKLGDLAFVDVTGRVAHAIMDLCGEPDAM
                     THPEGMQIKVSRQELSRLVGCSREMAGRVLKVLEEQGLLR"
BASE COUNT          618 a         1264 c         1299 g          661 t
ORIGIN      
        1 cagtcgtttc tgatgcttac gatgcatgag ggggatgtat gtcggcggac gctgacacga
       61 agaaaagacg cgcggccgcc gcggcgctcg agctggtggc ggatgagagc atcatcggca
      121 tcggcaccgg ctcgaccgtg aatctcttca tagacgcgct cgcgcggctg ccgcaccgcc
      181 cgcgccgcgc ggtatcgagc tcgcaggcga gcaccacgcg gctcgccgct atcggcatcg
      241 aggtgcgcga cttgaacgag gtgagcgagc tcaacctcta tttcgacggc gccgacgagg
      301 caacccccgc acggcagctc atcaagggtg gcgggggtgc gctgacgcgc gagaagatca
      361 tcgcggccgc cgcgcggcgc ttcgtgtgca tcgtcgacga cagcaaggtg gttgagaccc
      421 tcggccgttt ccccctgccc atcgaggtga tccccatggc ccgcaccctg gtgtcgcgcg
      481 agctcaccgc gcgcggtgcg cggccgttgt ggcgcgaggg attcgtcacc gacaacggca
      541 accacatcct cgacgtccat ggtctcagga tcaccgaccc ggcggcgctc gagcgtgagc
      601 tcaacctgat acccggagtg gtcaccgtcg gcctgttcgc ggtgcgtccg gcggacgtgc
      661 tgatcgtcgg caccgcgcgg ggcgtcgaga cccgctgatc ctcagtgcag gttgctggcg
      721 acgaagtccc agttcaccag ctgccagaaa ctcctgaggt atgcgccacg gtcgttgcgg
      781 tgatccacgt agtaggcgtg ctcccaaacg tcgcaggcga gcagcggggt atgtccgtcg
      841 cgcaacggcg tgtgcgcgtt ggcggtggtc acgatccgca ccgcgccgct gcggtccttg
      901 atgagccagg cccagccgga gccgaacacg gcgagtgccg cgcgggagaa ctcgcgctcg
      961 agtgcgtcca gcccgccgaa gctgcgcagc gccccctcga gctccctgcc gggcgcctgg
     1021 cggcgcggtg acaggcacct ccagaagaac ccgtggttcc acgcctgcgc ggcgttgtcg
     1081 aacagcgcgc cgtcggtgcg catcaccagc tcctcgagcg tcgcctgctg ctgcggggtg
     1141 ccgcggatca gcatattcac gttgtcgacg taggccttgt ggtgcttgcc gtagtggtag
     1201 tcgagggtct cgcgcgacat gtagggctcg agagcctggt gctcataggg gagcgggggg
     1261 agactgatcg tcgattcagt cgccataggg aacctcctgg cattgcgcca tccgtgcggt
     1321 gcggcgctgc gcccattgga cagcagagtg gtggtgaagt gcaaaaccgg cggtcgaacc
     1381 tgcggttctc atcctgcgga taaaaaaagg cccgtctgcg tagacgggcc ttccttcatt
     1441 ggctggggga ctaggattcg aacctaggta aacggagtca gagtccgttg tcctaccact
     1501 agacgatccc ccaagaggga tccctcgacc ttaacgcttc gagtactgcg tcccgcggcg
     1561 tgccttgcgg cggccgacct tcttgcgctc cacctcgcgc gcatcgcggg tcacgaagcc
     1621 cgcatcgcgc agcggcttgc gcaggctctc atcataatcg atgagcgcgc gggtgatccc
     1681 gtggcggatc gctcccgcct ggccggtgat gcccccgccg tcgacctgta ccgagatgtc
     1741 gaacttggtg tgcagcttca cggcgtcgag cggctgccgc acgatcatgc ggccggtctc
     1801 acgtccgaag aactcatcca gcgaacggtc gttgacggtg atcttgccgc tgccgggctt
     1861 caagtacaca cgcgccgtgg cggtcttgcg ccggccggtg ccgtagtgat tcggggtcgc
     1921 tggcattgag tgggtcctat tgaatctcga gaggcttcgg ctgctgcgcc tggtgcggat
     1981 gctcaccgcc ggcgaacacg tggagcttcc tgaacacgcg ccggccgaga gggttcttcg
     2041 gcagcatgcc cttgaccgcg aactcgatcg cgcgctcggg gtgctcggcg agcagcttcc
     2101 cgagggagat cgacttgata ccgccgatgt ggccggtgtg gtgatagtag atcttgtcgg
     2161 tgagcttgcg cccggtgacc cggatcttct gcgcgttcac caccacgatg tgatcgccca
     2221 tgtcgacgtg ggggctgtag tcagccttgt gcttgccctt caggcgatgg gcgatctgcg
     2281 aggcaaggcg ccccagtgtc ttgtcgctgg cgtcgacgat gaaccagtcg ccgcggacgc
     2341 tcgcgggctt ggcaaatacg gtcttcatag gtcgctcgtg gctgcgggcc tgcggccggg
     2401 ggttaacccc tcgagggccg gggccaaaaa aaggcgggca agtttactca agcgcctgcc
     2461 gcaatgcaaa gcagcgcgtc ctttcccgca cggcccgcct ccgggggagg tcctgagcct
     2521 atgggagcgg cgcccgagcc tataatgagc cctagtatat ggagtccggc cgctctctag
     2581 acgctgcgat cgccgccgtg gatcgtgccc tgcggtcagt ctttgcgccg gcccgggccg
     2641 cccggcccct cccgggcacc ccccagaagg aagccccgcc gctgaccgcc gcgcagcaac
     2701 gtgagagcgc ggcactcatg cgcgtcaatc actccggcga gctcgccgcc caggcgctct
     2761 accatggaca ggcgctggtg gcgcgctccg aggccatccg cgccttgctg ctcgaggcgg
     2821 cgcgctccga agcggatcac ctggcgtggt gcgagacgcg tctcgcggag ctgaacgcac
     2881 ggcccagcct gctcaatccg ctctggtatg tcgggtcttt cgccatcggc gcgctcacgg
     2941 cgctggccgg cgatcgcacc agtctcgggt tcgtcgtcga gacggaacgc caggtcgaag
     3001 gccacctgga cgagcacctc gacagactcc cggtaggcga cagccgcagc cgtgccatac
     3061 ttgaaaccat gagagcggaa gagattgcgc acggtgcaac ggcggccgcg gccggcggcg
     3121 cgcccctgcc cgcgccggtg cgcgcactca tgcggcagac ggcccgcgtc atgacgcgga
     3181 cggcctactg ggtctgacgg gcggcgaagg ctatattaaa gatgtgatcg gggggcctgg
     3241 tatggaagag aacatcaagc cgctcagcga gattccggca atcaaccgct tcctgagcta
     3301 ctgccgcatt cgcgcggtgc cgtccaagac tgtggtaatc cacgccggcg acctgccgga
     3361 cgtcctctac tacatcatca gcggctcggt cgaggtgatg atcgaggatg aggagggcaa
     3421 cgagatggtg ctcgcctacc tcaaccgcgg ccagttcttc ggcgagatgg gcctgttcta
     3481 cgagcagccc acccgcagcg cctgggtccg cacccgcaac cagtgcgagc tcgccgagat
     3541 gacctatccg cgtttccgcc agatcgccgc cgagagtccc gggctcgtgt tcgagctcgc
     3601 cacgcagctc gccacccgcc tcgatcgcac caaccgcaag ctcggcgatc tggctttcgt
     3661 cgatgtcacc ggacgcgtgg cgcacgcgat catggatctg tgcggcgagc cggatgccat
     3721 gacgcatccc gagggcatgc agatcaaggt gagccgccag gagctctcgc gcctggtggg
     3781 ctgctcacgc gagatggcgg gacgcgtgct caaggtgctc gaggaacagg gtctgctgcg
     3841 cg
//
LOCUS       DATLKL010000519         2235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_96412, whole genome shotgun sequence.
ACCESSION   DATLKL010000519 DATLKL010000000
VERSION     DATLKL010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96412"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..676)
                     /locus_tag="VM713_06975"
     CDS             complement(128..676)
                     /locus_tag="VM713_06975"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HVH84041.1"
                     /translation="MFIQTEHTPNPATLKFLPGRQVMASGTANFPSPETADRSPLAER
                     LFQIDGVTGVFLGADFITVAKADDREWHLLKPAILGVIMEHFVAGRPVILAADAPPSE
                     TEGAEDEIVVQIKELLDTRVRPAIAQDGGDIIFHDYQDGVVYLHMQGSCSGCPSSTAT
                     LKAGIENMLRHYLPEIVEVRAV"
     gene            875..2116
                     /locus_tag="VM713_06980"
     CDS             875..2116
                     /locus_tag="VM713_06980"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVH84042.1"
                     /translation="MDEGANSTSHHAISLSLPEPIPWLLTEARRIADGETFLARLCER
                     LLAVGLPLYRATIGVRVLHPQLIGVNLLWRRGSEVERIERTHGTELTSTYLESPIRVL
                     YEGAAALRRRLEGPDAQLDFPILKELRAEGVTDYVAMAMPDMEGQPASVTWASDRPGG
                     FSTADLTLLWDILPILSLSIEVRKVRRLAGNLLDFYLGQQTGRRVLQGAIRRGELETI
                     HAALWYSDLRGFTAMSDTLPFVELIELLNAYFEAVSEPVMARGGEVLKFIGDAMLAIF
                     ALDPAREGEGVRLAMEAAEEALRNVERVNGTRAAQGKRAFRLGIALHVGDVGYGNIGA
                     PTRLDFTVIGQAVNKVVRIEALSKALDRPLLVSSEFAHRCTHPLISLGFHALRGISEP
                     EEIFACPHAEEGVRTAAAKRA"
BASE COUNT          385 a          735 c          703 g          412 t
ORIGIN      
        1 gtgtcgccgg tcattcgcgg accgacggca ccatagccac aggcgcccag ttcggccgaa
       61 cgcgcgtctc tccccgtgcg cttcgtcggc gcaattacgg cgcagcagtt gtctgagcta
      121 ggcagcgtca aacggccctg acttccacga tctcaggcag gtagtggcgc agcatgttct
      181 caatccctgc cttgagcgtt gcagtcgagc tcgggcatcc cgagcaggag ccctgcatgt
      241 gcaagtacac gactccgtcc tggtagtcgt ggaaaataat atcgccaccg tcctgcgcga
      301 tcgcggggcg cacccgcgta tcgagcagct ccttgatctg cacgacaatc tcgtcttccg
      361 ccccctcggt ctcgcttggc ggagcatccg cggcgaggat caccggccga cccgcgacga
      421 agtgctccat aattacgccc aggattgcgg gtttgagcag atgccactca cggtcgtcgg
      481 ccttggcaac ggtgatgaag tccgcaccca aaaagacgcc cgtcacgccg tcgatctgga
      541 acagccgttc cgccaacggc gaccgatccg ccgtctcagg cgacggaaaa ttagcagtcc
      601 cagaggccat gacttggcgg ccaggcaaga acttgagcgt tgctgggttc ggtgtgtgct
      661 cagtctgaat gaacatgact tcctcgttaa ccctggtcga cgcgttaact ccaaaccttc
      721 tttttctgtt aaccgtttcg ccgcgatttt cgatgcaagg cgcctcctta gatggagcat
      781 acgtcacgga aaatcaaggc aaattcgaac gaaccgttaa ccctgttcgt gccgggtacg
      841 cagctgatgt gattccagca acgagggaaa ttcgatggac gaaggcgcga actcaacctc
      901 acatcacgca atcagcctgt cgcttcctga gcccatcccg tggctgctga cggaagcccg
      961 ccggatcgcc gacggcgaga ctttcttggc gcgtctttgc gagcgcctgc tcgcagtcgg
     1021 gctgccactc taccgcgcga cgatcggcgt gcgcgtgctc catccgcaac tcatcggcgt
     1081 gaacttgctg tggcgacgtg ggtccgaggt ggagcggatc gagcgcacgc acggcaccga
     1141 gttgacgagc acctacctcg agagccccat tcgcgtgctc tatgaggggg cagcagcgct
     1201 ccgacgccgg ctcgaagggc ccgatgccca gctcgatttt cccatcctca aggagctgcg
     1261 cgccgagggc gtcaccgact atgtcgcgat ggcgatgccc gacatggagg gccagcctgc
     1321 gtcggtcacc tgggcctccg accgtccggg cggattttcg acggcggacc tgacgctgct
     1381 ctgggacatc ctgcccatcc tgagccttag tattgaggtg cgcaaggtgc gccgtctcgc
     1441 gggtaacctg ctcgacttct acctcggaca acagaccggc cggcgcgtgc tgcagggagc
     1501 catcaggcgg ggcgagctcg agaccatcca cgcggcgctg tggtattccg acctgcgcgg
     1561 cttcaccgcg atgtcggaca cgctgccgtt cgtcgagctg atcgagctct tgaatgcgta
     1621 cttcgaggcg gtgtcggagc cggtcatggc gcgcggtggc gaggtgctca agttcatcgg
     1681 cgacgccatg ctcgcgatct tcgcgctcga tcccgcgcgc gagggcgagg gtgtccggct
     1741 ggcgatggag gccgcggagg aggcattgcg caatgtcgag cgcgtcaatg ggacgcgcgc
     1801 ggcccagggc aagcgcgcat tccggctcgg catcgcgctg catgtcggcg atgttggcta
     1861 cggcaatatc ggggcaccaa cgcggctcga cttcaccgtg atcggccagg ccgtcaacaa
     1921 ggtcgtgcgc atcgaggcgc tgtcgaaggc gctcgatcgc cctctcctcg tgtcgtccga
     1981 gttcgcgcat cgctgcaccc atcccctgat ctcgctcggc ttccacgccc tgcgcggcat
     2041 ctccgagccg gaggagatct tcgcttgccc gcacgcggag gagggcgtgc ggaccgccgc
     2101 ggcgaagcgc gcgtaatctg ttcggccctc gcggtcgagg tcccgctctc ccgcctcggc
     2161 ggaagcatcg acgcaagctc aacgcagcat cgatccgctt cagcttcgcg caggcaggca
     2221 aggagcgcgg gaccc
//
LOCUS       DATLKL010000520         1877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_197908, whole genome shotgun sequence.
ACCESSION   DATLKL010000520 DATLKL010000000
VERSION     DATLKL010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197908"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1299)
                     /locus_tag="VM713_06985"
     CDS             complement(16..1299)
                     /locus_tag="VM713_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH84043.1"
                     /translation="MTQQPAAPELARGTRWWIVVLLATALFINYVDRGAVPTAAHLIQ
                     DDLGFSPQQLGLLLSAFWWLYALLQFPVGWLVERYGAQRLLGAGLALWACATMLVGVV
                     HSFGALLALRLLLGVGESVGFPSVSKLLATVVPVKSLGLANGIVAFGYLFGPAVGSLV
                     GGMLMARYGWRSSFWVFGALSLLWLLPWARVRLPARPAAASAGTDPPLRALLERRELW
                     GTSLGLFSVNYAFYFTLTWLPFYVVREHGFSTEKMAGFTFLAYVVHAVSGLAAGWLID
                     RMVARREHASLAYKAVMGATHIGFVACMVCIALAPAPWALAAIFAYQVLDGANSPGVF
                     GIPQILAGPRAAGRWVGIQNGIGNFAGVIAPALTGFLVGHSQHFTSAFLVAAVVSVLG
                     VVGWVFMIPRVAPLEWPGYDATNTPRPAGSAPSVA"
     gene            complement(1296..1790)
                     /locus_tag="VM713_06990"
     CDS             complement(1296..1790)
                     /locus_tag="VM713_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HVH84044.1"
                     /translation="MHASAHRLCPQARCVLHTHMPYATALGMLADARLETASQSAIGF
                     HGDVAYVDYNGLALDFAEGERLARALGSKSVLVLRNHGVLVTGDAAAQAFERLYFLER
                     ACQVQVLALATGRPLGVLPEPVVRATLAQFARCGSVGGQDRAQLHFEALKRLLDRNGA
                     DYAS"
BASE COUNT          349 a          641 c          663 g          224 t
ORIGIN      
        1 cgggaccgcc gtgcgctacg caaccgaggg agcgctccct gccgggcgcg gcgtgttagt
       61 tgcatcgtac ccgggccact cgagcggggc gactctcggg atcatgaaga cccagcccac
      121 caccccgagg acgctgacga cggcggcgac gagaaaggcc gacgtgaagt gctggctgtg
      181 cccgaccaga aatccagtca gcgccggggc gatcacgccg gcaaagtttc ctatgccgtt
      241 ctggattccg acccagcggc cggcggcacg cggtccggcg agaatctgcg gtattccgaa
      301 caccccgggc gagttggcgc cgtcgagcac ctggtaagcg aagatcgcgg cgagcgccca
      361 cggggctggc gccagcgcga tgcataccat gcacgccacg aagccgatgt gggtggcgcc
      421 catgacggcc ttgtaagcga gactggcgtg ctcacgtcgg gcgaccatgc ggtcgatgag
      481 ccaaccggcg gcgagtccgc tcacggcgtg cacgacatag gcgaggaagg tgaacccggc
      541 catcttctcg gtcgagaagc cgtgctcgcg caccacgtag aagggcagcc aggtgagcgt
      601 gaaatagaaa gcgtaattga ccgagaagag cccgaggctc gtcccccaga gctcgcgccg
      661 ctcgagaagc gcccgcaggg gcggatcggt gcccgcgctc gccgccgccg gccgcgccgg
      721 cagccgcacg cgcgcccatg ggaggagcca cagcagcgac agggcgccga agacccagaa
      781 cgagctgcgc cagccatagc gcgccatgag catgccgccg accagtgagc cgaccgcggg
      841 cccgaagagg tagccgaacg caacgatgcc gttggcgagc ccgaggctct tcaccggcac
      901 gaccgtcgcc agcagcttcg acaccgaggg aaagccgacg ctctcgccga caccgagcag
      961 cagccgcagc gcgagcaacg caccgaagga atgcacgact ccgaccagca tggtcgcgca
     1021 ggcccagagc gcgagccctg cgccgagcag gcgctgcgcg ccgtagcgct cgaccagcca
     1081 gccaaccggg aactgcagca gcgcgtacaa ccaccagaac gcggacagca gcaagccgag
     1141 ctgctggggc gagaagccga gatcgtcctg gatcaggtgc gcggcggtcg gcaccgcgcc
     1201 acggtcgacg tagttgatga acagcgccgt ggccagcagc accacgatcc accagcgcgt
     1261 gcccctagcg agctcgggcg ctgccggctg ctgggtcaac tcgcgtagtc cgcgccgtta
     1321 cgatccagga ggcgcttgag agcctcgaag tgcagttgcg cgcgatcctg accgccgacg
     1381 ctgccgcagc gggcgaactg agcgagcgtc gcgcgcacga ccggctcggg tagtacgcct
     1441 aaggggcgcc cggtcgcgag cgccaggacc tgtacctgac acgcgcgctc gaggaagtac
     1501 agccgctcga acgcctgggc cgccgcgtct cccgtcacca gtacgccgtg gttgcgcaac
     1561 accagcaccg acttgctgcc gagcgcgcgc gccaggcgct cgccttcggc aaagtcgagg
     1621 gcgaggccgt tgtagtcgac gtaggcgacg tcgccgtgga agccgatggc gctctgcgag
     1681 gcggtctcga ggcgcgcatc cgcgagcatg ccgagcgcgg tcgcataggg catgtgggta
     1741 tgcaggacgc agcgcgcctg ggggcagagc cggtgggcag aggcgtggat atagaggggt
     1801 cgccagcact tcgtcgatgc gcttctcggt cacgccgccg agcagcctgc caaacgcgcg
     1861 gaggttgtct ctgcccg
//
LOCUS       DATLKL010000521         2469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_107569, whole genome shotgun sequence.
ACCESSION   DATLKL010000521 DATLKL010000000
VERSION     DATLKL010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107569"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VM713_06995"
     CDS             complement(<1..916)
                     /locus_tag="VM713_06995"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HVH84045.1"
                     /translation="MKTSSVQRLEAVAPAEQSALSLPAWIYQDAEFFEREQQAIFRHA
                     WQLVCHLSDIPRPGDFHTFEYLGESVVVLRAEEGAVRAFHNVCRHRAARLLDGPSGHC
                     GRRITCPYHAWTYALDGRLIGVPQRDQFPGLDTAQHGLKPLEHEVFMGFVFVRFAPGL
                     PSVHEMAAPYQHELAAYRLEELVPMGRVTLRPRTVNWKNVADNYSDGLHINVAHPGLT
                     RLFGKGYGIEARPWIDKMWGILRDTPSSRWSERMYQSVLPPVAALPPERQRLWTYFKL
                     WPNLAFDIYPDQIDFVQFLPVSPTETMIR"
     gene            1048..1470
                     /locus_tag="VM713_07000"
     CDS             1048..1470
                     /locus_tag="VM713_07000"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HVH84046.1"
                     /translation="MDIAHVVQSSVAPVFLLTGVAATLSVLTNRLARVVDRARALEGQ
                     LEREPTRRGVLLADLRNLERRAGYINAAISMSAVAALLVALVVVSLFADAFLGVNLAM
                     AVAVLFTGSMLCLAVAYVAFFVEVRIAVAALRIGIPHK"
     gene            1587..>2469
                     /locus_tag="VM713_07005"
     CDS             1587..>2469
                     /locus_tag="VM713_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HVH84047.1"
                     /translation="MHRPIRNIAIIAHVDHGKTTLVDCLLKQSGTFAEHERLGERIMD
                     SNDLERERGITILAKNCAIEYGGTRINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALAQGLKAIVVVNKIDRPGSRPAWVIDQTFDLFDKLGTTDAQLDF
                     PVIYASALQGWASTDYTERRPDMSALFEAILTHVSAPAADAERPLQLQISTLDYNSYV
                     GRIGIGRIRRGVVRPGESVALRFGNEDRGTARTGQVLSFSGLERVPVTEAGAGDIVAI
                     TGIESVNIGMTLCDPEHP"
BASE COUNT          410 a          825 c          827 g          407 t
ORIGIN      
        1 cgcggatcat ggtctcggtg ggggagaccg ggagaaactg cacgaaatcg atctgatcag
       61 gatagatgtc gaaggccagg ttcggccaca gcttgaaata ggtccacagc cgctgccgct
      121 ccggcggcag cgccgcgacc ggcggcagca cgctctgata catgcgctcc gaccatctgg
      181 atgagggcgt atcgcgcaga ataccccaca tcttgtcgat ccacggtcgc gcctcgatgc
      241 cgtagccctt gccgaacagg cgcgtgagcc ccgggtgggc cacgttgatg tgcaggccgt
      301 cggaatagtt gtcggcgacg ttcttccagt tcaccgtgcg cggccgcaag gtcacgcggc
      361 ccatcggcac gagctcttcg agccggtacg ccgcgagctc gtgctggtac ggcgccgcca
      421 tctcgtgcac gctcggcagc cccggcgcga agcgcacgaa aacgaagccc atgaacacct
      481 cgtgctcgag gggcttaagg ccgtgctgcg cggtgtcgag gccggggaac tgatcgcgct
      541 gcgggacgcc gatcagccgc ccatcgagcg cgtaggtcca ggcgtggtag gggcaggtga
      601 tgcgccggcc gcaatgcccg ctcggcccgt cgaggaggcg cgccgcgcgg tgccggcaga
      661 cattgtgaaa ggcgcgcacc gcgccctcct cggcccgcag caccacgacc gactcgccga
      721 ggtactcgaa ggtatgaaaa tcaccgggac gggggatgtc gctcaggtgg cagacgagct
      781 gccaggcgtg gcggaagatc gcctgctgct cgcgctcgaa gaactccgcg tcctggtaga
      841 tccaggcggg gaggctgagc gcgctctgct cggcgggcgc gacggcctcg aggcgctgca
      901 cggagctcgt cttcatggcg ggcaaacccg ggttccttac cttgttgaac gatcgtacaa
      961 cagccagaac gagggccgca atcgccgcgc ggtttatgat agaagacgcg cgctgcggtg
     1021 agcgctgcgt ctctttcagg gggctcgatg gatatcgcgc acgtcgtcca gtcgtcggtg
     1081 gcacccgtct tcctcctcac gggcgtcgcc gccacgctca gcgtgctcac caaccgcctc
     1141 gcgcgcgtgg tcgatcgggc gcgcgcgctc gaggggcagc tcgagaggga gccgacacgc
     1201 cgcggggtcc tgctcgccga cctcaggaac ctcgagcgtc gtgccggtta catcaatgca
     1261 gcgatctcga tgtccgcggt cgcggcgctg ctcgtggcgc tggtcgtggt ctcgctgttc
     1321 gccgacgcat tcctcggagt caacctcgcc atggcggtcg cggtgctctt cacaggctcg
     1381 atgctgtgcc tcgccgtcgc ctacgtcgct ttcttcgtcg aggtccggat tgccgtggcg
     1441 gcgctgcgca tcggtattcc ccacaagtag ataaatagca ttaaatcaat cagttaagat
     1501 tgaaaccggc gcctgtttcg gcgttctggt accctgcgca cccttttttc gatgccgcac
     1561 cgctcgccga acgcggcgct tccgaaatgc atcgtccgat ccgcaacatc gccatcatcg
     1621 cgcacgtcga tcacggcaag acgacgctcg tcgactgcct gctgaagcag tcggggacct
     1681 tcgccgagca cgagcggctc ggcgagcgca tcatggattc caacgacctc gagcgcgagc
     1741 gcggtatcac gatcctcgcc aagaattgcg ccatcgagta cggcggcacg cgcatcaaca
     1801 tcgtcgatac cccgggacac gccgacttcg gcggcgaggt cgagcgggtg ctgtcgatgg
     1861 tcgatggggt gctgctgctg gtcgatgcgg tcgaggggcc gatgccgcag acgcgcttcg
     1921 tcacgcgcaa ggcgctcgcg caggggctga aggcgatcgt cgtggtcaac aagatcgacc
     1981 gccccgggag ccgtccggcc tgggtcatcg accagacctt cgacctgttc gacaagctgg
     2041 gcacgaccga tgcgcagctc gatttcccgg tgatctacgc ctcggcgctg cagggctggg
     2101 cgagcaccga ctacaccgag cgccgcccgg acatgtcggc cctattcgag gcgatcctca
     2161 cgcatgtgtc ggcaccggcg gcggacgccg agagaccgct gcagctgcag atctcgacgc
     2221 tcgactacaa ctcctacgtc gggcgcatcg gcatcggccg catccgccgc ggcgtcgtgc
     2281 ggcccggaga gagcgtggcg ctgcgcttcg ggaacgagga ccgcggcacg gcgcgcaccg
     2341 gccaggtgct cagcttctcc gggctcgagc gcgtaccagt cacggaagcc ggcgccggcg
     2401 acatcgtcgc gatcaccggc atcgagtcgg tcaacatcgg catgacgctc tgcgacccgg
     2461 agcaccccg
//
LOCUS       DATLKL010000522         1651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_17221, whole genome shotgun sequence.
ACCESSION   DATLKL010000522 DATLKL010000000
VERSION     DATLKL010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17221"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VM713_07010"
     CDS             complement(<1..910)
                     /locus_tag="VM713_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84048.1"
                     /translation="MAGVAVSAAVATAAPAVELDDIQGLVRFAYKNHTEAVFLLLRVK
                     EAIAARAWLAGASVADAVIKAPPAATALQVGFTSEGLRALGIGADVIEGFSQEFVNGM
                     AGDASRSRRLGDIGLSDPSRWQWGSGAAAPHAVALLYALPGRLAEWQRGIEEELAAGF
                     EIMNRLATSDMGGVEPFGFVDGISQPELDWKRQRAARDTTELAYVNLSCLGEFLLGYR
                     NEYGLYTTRPLLDPSADTLGVLPPAEDAPDKLDLGRNGTYLVIRQLRQNVPAFWQALD
                     RYAGGVASVREELASAMVGRHMNGEPL"
     gene            complement(892..>1651)
                     /locus_tag="VM713_07015"
     CDS             complement(892..>1651)
                     /locus_tag="VM713_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84049.1"
                     /translation="TYVESERAAGRLLVTPPEPTPLTWRLARLANLVLGLAVAIMALP
                     FLIVLAPIVIVWLRILETTDPEICPPPDPAALLELQEIEDFDVTNQYTAFGSAKPGWF
                     RRGLLTVVLVAIEFTCRHVFTRGYLARVQTIHFARWVFLDNKSRVMFNSNYDGGHQAY
                     MDDFINKVGWGLNLVFSNGLGWPRTDWLITRGARRELQFKFYQRRHQLPTQVWYKAYP
                     GLTLIDMTRNSRIRQGLENGRMSDREVASWLALL"
BASE COUNT          287 a          551 c          552 g          261 t
ORIGIN      
        1 tcagcggctc gccattcatg tgccgcccca ccatggcgct cgccagctcc tcgcgcacgc
       61 tcgcgactcc tccggcgtag cggtcgagcg cctgccagaa ggcgggcacg ttctggcgca
      121 gctgccggat cacgaggtag gtgccgttgc ggcccaggtc gagcttgtcc ggcgcatcct
      181 ccgcgggcgg cagcacgccg agagtgtcgg ccgagggatc aagaagcggc cgcgtcgtgt
      241 agagcccgta ctcgttgcga tagccgagca ggaactcgcc aaggcagctc aagtttacat
      301 acgccagctc agtcgtatct cgtgccgcac gctgccgctt ccaatcgagc tcgggctgcg
      361 agatgccatc gacgaagccg aagggctcca ccccgcccat gtcggaggtg gcgagccggt
      421 tcatgatctc aaagccggcc gcgagttctt cctcgatgcc ccgctgccac tcggcgagcc
      481 ggcccggcag agcgtagagc aacgcgaccg catggggcgc ggccgcgccg cttccccatt
      541 gccagcgaga gggatcgctt aagccgatgt ctcccaggcg ccgtgagcgg ctcgcgtctc
      601 cggccatgcc gttcacaaac tcctgcgaga agccctcgat gacatcggcg ccgatgccga
      661 gtgcccgcag gccctcgctc gtgaagccaa cctgcagcgc cgtggcagcg ggcggtgcct
      721 tgatgaccgc atcggcgacg gacgcacctg cgagccaggc gcgggcagct atggcctctt
      781 tcacccggag cagcagaaac accgcttcgg tgtggttctt gtacgcgaaa cgcaccagtc
      841 cctggatgtc atcgagctcg acggccggcg ccgcggtcgc tacggcagcg ctcacagcaa
      901 cgccagccac gacgccacct cgcggtcgct catgcgcccg ttctcgagcc cttgtcggat
      961 gcggctgttg cgcgtcatgt cgatgagggt gagaccgggg tacgccttgt accagacctg
     1021 agtggggagc tgatgccgcc gctggtagaa cttgaactga agctcgcgcc gcgccccgcg
     1081 ggtgatgagc cagtcggtgc gcggccagcc gaggccgttg ctgaaaacaa ggttcaggcc
     1141 ccagcccacc ttgttgatga agtcgtccat gtaggcctgg tgaccgccgt catagttgct
     1201 gttgaacatg acgcgcgact tgttatcgag aaacacccaa cgggcgaagt gaatcgtttg
     1261 cacgcgagcg aggtacccgc gggtgaagac gtggcggcat gtgaactcga tggccaccag
     1321 cacgaccgtg aggagcccgc gccggaacca gccgggcttg gccgagccga aggcggtgta
     1381 ctgattggtg acgtcgaagt cctcgatctc ctgcagctcg agaagcgctg ccgggtcggg
     1441 cggcggacag atctccggat ccgtcgtctc gaggatgcgc agccacacga tcacgatcgg
     1501 cgccagaacg atgaggaacg gcagcgccat gatcgcgacg gccaggccca gcacgagatt
     1561 ggcgagccgc gcgagccgcc acgtgagggg agttggctcg ggcggcgtca cgagcagccg
     1621 gcccgccgcc ctttcggact cgacatacgt g
//
LOCUS       DATLKL010000523         2548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_85103, whole genome shotgun sequence.
ACCESSION   DATLKL010000523 DATLKL010000000
VERSION     DATLKL010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85103"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VM713_07020"
     CDS             complement(<1..552)
                     /locus_tag="VM713_07020"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HVH84050.1"
                     /translation="MSGPRTRAAVAVPSPGEARRPRFQRQLPQQRRGALIAATIECLK
                     RYGHDGLSIRTISAQAGVSVGLINHHFPNKDELVAAAFRHFNRELVDGLRAAAMRVES
                     PPARLRAFLEASFSPPNLDADVLAVWVVFWGLYRHSRPIQRVQRETYTDYVRLVRGLL
                     GDLLGKAADGGKSGARGGGVNLRL"
     gene            complement(549..1355)
                     /locus_tag="VM713_07025"
     CDS             complement(549..1355)
                     /locus_tag="VM713_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVH84051.1"
                     /translation="MLVEGGRAVGVVTESGETLRAAAVISNLNPKLLYLEMFEPDALP
                     PEFRERIGYWRCGSGTFRMNVALAELPDFACLPGRRPADHHTAGIILAPTLAYMERAY
                     FDARALGWSRAPIVELVIPSTLDTSLAPEGQHVASLFCQHVAPQLPNGASWDAHREAV
                     ADLMIDTVNAYAPNFKAAVLGRQIMSPLDLERTFGLVGGDISHGVMSLDQLFSARPML
                     GYGNYRGPLAGLYMCSAGTHPGSGVTGAPGHNAAREILADFRRGRLRRLG"
     gene            complement(1271..2188)
                     /locus_tag="VM713_07030"
     CDS             complement(1271..2188)
                     /locus_tag="VM713_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVH84052.1"
                     /translation="MAEADTAGGRCDALIIGAGHNGLTCAAYLAAAGLKVTVLERRAI
                     VGGAAVTEEFHPGFRNSVAAYTVSLLNPKVIRDLELPRYGLRIVERRCANFLPTADGS
                     YLMTGEGRTQTEVARFSARDAARIPEYGARLDSIADVLRDLVLVTPPNAVAGSWRTAL
                     PELVRAAQVGGRLRRLDMSLRRELLSLFAMSAGDYLDRWFQSDPIKAVFGFDGIVGNY
                     ASPYSGGSAYVLLHHAFGEVNGKKGAWGHALGGMGAITQAMAQSASARGAQIRTNAPV
                     RRCSSRAGARSGSSPNRVRRCARPPSSPT"
     gene            complement(2181..2531)
                     /locus_tag="VM713_07035"
     CDS             complement(2181..2531)
                     /locus_tag="VM713_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HVH84053.1"
                     /translation="MYQINWRKIVIDQIDFMQFLPVSPTETMIREIAYVHADARREMR
                     LARYLNWRINRRVSLEDQALIERVQAGMASSSYTMGPLSEGEVCLRSFARRMRALIPE
                     SRLPHPPARGWSRG"
BASE COUNT          409 a          881 c          904 g          354 t
ORIGIN      
        1 gagccgcaga ttcacgccgc cgcccctagc cccgctcttg ccgccgtcgg ccgccttgcc
       61 gagcaggtcg ccgagcaggc cgcgcacgag gcgcacgtag tcggtgtagg tctcgcgctg
      121 gacgcgctgg atcgggcgcg aatgccggta caggccccag aacaccaccc acaccgcgag
      181 cacgtcggca tcgagattcg gcggcgagaa agaggcctcg aggaacgcgc gcagccgcgc
      241 cggcggcgac tcgacgcgca tggccgcggc cctgagaccg tccaccagct cgcggttgaa
      301 gtggcggaag gccgctgcca ccagctcgtc cttgttggga aagtggtgat tgatgagtcc
      361 gaccgacacg cccgcctgcg cgctgatggt gcggatcgag agaccgtcgt ggccgtaacg
      421 cttcaagcac tcgatcgtgg ccgcgatgag cgccccgcgc cgctgctgcg gcagctggcg
      481 ctggaagcgc ggccggcgcg cctcgcccgg actcggcacc gccacggcgg cgcgggtgcg
      541 cgggccactc acccgagccg ccgcagccgt ccgcggcgga agtcggcgag gatctcgcgc
      601 gccgcattgt ggccgggcgc gccggtgacg ccgctcccgg ggtgcgtgcc ggcgctacac
      661 atgtagagcc ccgcgagcgg gccgcggtag ttaccgtagc cgagcatcgg gcgcgccgag
      721 aacagctgat cgagactcat caccccgtgg gagatgtcgc cccccacgag gccgaaggta
      781 cgctccagat cgagcggact catgatctgg cgcccaagca ccgcggcttt gaagttcggg
      841 gcgtaggcat tcacggtgtc gatcatgaga tccgcaactg cctcgcggtg cgcgtcccac
      901 gaggcgccgt tcggcagctg cggcgccaca tgttgacaga aaaggctcgc cacgtgctga
      961 ccctcgggcg cgaggctggt gtcgagcgtc gaggggatga ccagctcgac gatcggggcg
     1021 cgcgaccagc cgagcgcgcg tgcatcgaaa taggcgcgct ccatgtaggc gagcgtcggc
     1081 gcgaggatga tgccggcggt gtggtggtcg gccggccgcc tgccgggcag acaggcgaaa
     1141 tcggggagct cggcgagcgc cacgttcatg cggaacgtgc ccgagccgca gcgccagtag
     1201 ccgatgcgct cgcggaactc ggggggcaag gcatccggct cgaacatctc gagatagagg
     1261 agcttgggat tcaggttgga gatgacggcg gccgcgcgca gcgtctcacc cgattcggtg
     1321 acgaccccga ccgcgcgccc gccctcgacg agcacctgcg cacgggcgcg ttggtacgga
     1381 tctgcgcacc gcgcgcactg gccgactgcg ccatcgcctg ggtgatggca cccatgccgc
     1441 cgagcgcgtg gccccaggcg cccttcttgc cgttcacctc gccgaacgcg tggtgcagca
     1501 gcacgtaggc cgagccgccc gagtaggggc tcgcataatt gcccacgatg ccgtcgaagc
     1561 cgaacaccgc cttgatgggg tcgctctgga accagcggtc gaggtagtca ccggcggaca
     1621 tcgcaaacag cgacagcagc tcgcgcctca agctcatgtc gagccggcgc agccgccccc
     1681 ccacctgcgc agcgcgcacc agctccggca gtgccgtgcg ccagctcccg gcgaccgcgt
     1741 tcggcggcgt caccagcacg aggtcccgca gcacgtcggc gatcgagtcg aggcgtgcgc
     1801 cgtactcggg gatgcgcgcc gcatcgcgcg ccgagaagcg cgccacctcg gtctgcgtgc
     1861 gcccctcgcc ggtcatgaga taacttccgt cggccgtcgg caggaagttg gcgcagcgcc
     1921 gctcgacgat ccgcaggccg tagcggggga gctcgaggtc gcggatgacc ttcgggttga
     1981 ggagcgagac ggtataggcg gcgaccgagt tgcgaaagcc ggggtgaaac tcctcggtga
     2041 cggccgcccc gccgacgatc gcgcgccgct cgagcacagt caccttgagc ccggcggccg
     2101 cgagatacgc cgcgcaggtg aggccgttgt gtcctgcgcc gatgatcaac gcatcgcagc
     2161 gcccaccggc cgtgtccgcc tcagccacgg ctccagcccc gtgccggcgg atgcggcagc
     2221 cggctctcgg gaatcagcgc gcgcatgcgc cgcgcgaagc tgcgcaggca cacctcgcct
     2281 tcgctcagcg gacccatggt gtagctgctc gaagccatgc ccgcctggac gcgctcgatg
     2341 agcgcctggt cctcgaggct cacgcggcgg ttgatgcgcc agttgaggta acgcgcgagg
     2401 cgcatctcgc gccgcgcgtc cgcgtgcacg taggcgatct cgcggatcat ggtctcggtg
     2461 ggggagaccg ggagaaactg catgaaatcg atctgatcaa tgacgatttt tcgccaattg
     2521 atctggtaca gctagaatca aggccgtg
//
LOCUS       DATLKL010000524         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_209129, whole genome shotgun sequence.
ACCESSION   DATLKL010000524 DATLKL010000000
VERSION     DATLKL010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209129"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VM713_07040"
     CDS             <1..678
                     /locus_tag="VM713_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVH84054.1"
                     /translation="DSFLAAAARYQLMPVIDRWVVNHVIEALKPRAGVLEGKALAFTI
                     NFSGQSLDDDTFADFLIKSIGESGLDPDLFVFELTENATVAHLPRAEELMRRLRKLGC
                     GVALDDFGTGLSSLSCIRQLPITMLKIDGSFIRDVVKDKRAESMVRAMAELARSMSII
                     TVAEYIETEAISNHVAGLGVDYGQGFAIGRPIPLAELLDELPLPSEPQGPEPRASDIM
                     WYAGATP"
     gene            695..1966
                     /locus_tag="VM713_07045"
     CDS             695..1966
                     /locus_tag="VM713_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HVH84055.1"
                     /translation="MTSRSRTLIAVVAGAIFGFCGALASGVLAERSTEPAHAAPPAIL
                     PWEEASLFAEVYERIKRDYVEDVDDHALMERAVRGMVAALDPHSAYLDSDEFEEIRLS
                     TTGSYPGIGIEVVPADGAVKVLHPIDGSPAQRAGLRRGDEIVKIDGVDIGGDLASASA
                     HMRGASGSTVKLSVRRKGSAALLEFTLRRAQVEVSSVTRQTLEPGYGYLRITSFTETT
                     AEDVGHAVAQLKRDNEQGIKGLVLDLRDNPGGVLEAGVAVVDDFLARGVIVSADGRTP
                     EARFRMEATHHGDLLNGAPLVVLVNGGTASASEIVAGALKDHGRALLIGRKTYGKGSV
                     QTVMPLSHGGALKLTTSRYFTPSGASIQGKGIIPDIVEGGPKESPTDAGSDQPLALRD
                     PDVHTGLATLKTRGALAARAADEHLTRHLVP"
BASE COUNT          353 a          743 c          693 g          314 t
ORIGIN      
        1 gacagcttcc tcgccgccgc cgcgcgctac cagctgatgc cggtcatcga ccgttgggtg
       61 gtcaaccacg tgatcgaggc cctgaagccc cgcgccgggg tgctcgaggg caaggcgctc
      121 gccttcacca tcaatttctc cggtcaatcg ctcgacgacg ataccttcgc cgacttcctc
      181 atcaagtcga tcggcgagag cgggctcgat ccggacctgt tcgtcttcga gctcaccgag
      241 aatgccaccg tcgcgcacct gccgcgcgcc gaggagctga tgcgccggct gcgcaagctc
      301 ggctgcggcg tggcactcga tgatttcggc accgggctct cgtcgctgtc gtgtatccgg
      361 cagctgccga tcaccatgct caagatcgac ggcagcttca tccgcgacgt ggtcaaggac
      421 aagcgcgccg agtcgatggt gcgcgccatg gccgagctcg cccgcagcat gtcgatcatc
      481 acggtggccg agtacatcga gaccgaggcc atcagcaatc acgtcgccgg gctcggcgtc
      541 gactacggcc agggttttgc gatcggccgg ccgatcccgc tcgcggagct gctcgatgag
      601 ctgccgctgc cgagcgagcc gcagggcccg gaaccgcgcg cgagcgacat catgtggtac
      661 gcaggagcga cgccgtgaac ggagtttcga ggacatgacc agccgctcac ggacactgat
      721 cgcggtggtc gccggagcca tcttcggctt ctgcggcgcg ctcgcaagcg gcgtcctcgc
      781 ggaacgctcc accgagcccg cgcatgccgc accgcccgcc atcctcccct gggaggaggc
      841 gagcctgttc gccgaggtct acgagcgcat caagcgcgac tacgtggagg acgtcgacga
      901 ccacgccctg atggagcgcg cagtccgcgg catggtggcc gcgctcgatc cgcactccgc
      961 ctacctcgac agcgacgagt tcgaggagat ccgcctgagt accacgggct cctaccccgg
     1021 gatcggcatc gaggtggtgc ccgcagacgg cgcggtcaag gtgctgcatc cgatcgacgg
     1081 ctcgcccgcg cagcgcgcgg gcctccggcg cggcgatgag atcgtgaaga tcgacggcgt
     1141 ggatatcggc ggcgatctcg ccagcgccag tgcccacatg cgcggtgctt ccggcagcac
     1201 ggtgaagctc agcgtccgcc gcaaaggcag cgctgcgctt ctcgagttca cgctgcggcg
     1261 cgcccaggtc gaagtgtcga gcgtcacgcg gcagaccctc gaaccgggct acggctacct
     1321 ccggatcacg agcttcaccg agaccaccgc ggaggatgtg ggccacgctg tcgcgcaact
     1381 gaagagagac aatgagcagg gaatcaaggg cctggtactc gacctgaggg acaatcccgg
     1441 aggggtgctc gaagccggcg tcgccgtggt ggacgacttc ctcgcgcgag gcgttatcgt
     1501 gagcgccgac ggacgcaccc ccgaggcgcg cttccgcatg gaagcgaccc accacgggga
     1561 cctcttgaac ggcgccccgc tcgtcgtgct ggtgaacggc ggaaccgcgt ccgcctccga
     1621 gatcgtcgcc ggggccctca aggatcacgg ccgcgcgctg ctcatcgggc gcaagaccta
     1681 cggcaaaggc tcggtgcaga cggtcatgcc gctctctcac ggcggggcgc tgaagctcac
     1741 gacgtcgcgc tatttcaccc cctccggcgc ctcgatccag ggcaagggca tcatccccga
     1801 catcgtcgag ggcgggccga aggagtcccc cacggacgcc ggcagcgacc agccgctcgc
     1861 cctgcgcgat cccgacgtgc acaccggact tgcgacgctc aagacacgcg gcgccctcgc
     1921 cgcgcgtgcg gcggatgagc acctgacgcg ccacctcgtc ccctagttca ttggcaaaat
     1981 cgcctgcggc gattttgcgg acctcgctcc ggttgccgcc ggcaaaatgg cggggctcga
     2041 tacgcaccag gcccgatcac cgcagcagcc gagcacttcg taacaaggtc ggcgcacggg
     2101 ttt
//
LOCUS       DATLKL010000525         1505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_62600, whole genome shotgun sequence.
ACCESSION   DATLKL010000525 DATLKL010000000
VERSION     DATLKL010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62600"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1505)
                     /locus_tag="VM713_07050"
     CDS             complement(<1..>1505)
                     /locus_tag="VM713_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84056.1"
                     /translation="DHSAELQAQNEALLQAFAGINPPPAAPDAMLTDVPLKACRAQLA
                     NTFDRRDGGFGGAPKFPAPKAIERLLRDWRSSPAGDEAGLQSLYMAGFTLRHMGEGGI
                     CDQLGGGFCRYSVDERWMIPHFEKMLYDNAALLAVYANSSLGTADAFYTQVALETAGW
                     VLREMQAPEGGFYSSLDADSEGHEGKFYVWDREEVRRALSAEELAAFAPRFGLDREAN
                     FEGRWNLYVDATVEQIAASLQRPAAEVSALLASSRAKLLALRAQRVRPARDEKILTSW
                     NALMIRGLAIAARVLTAEQLAAAASRALDFVRATLWREGRLLATYKDGRAHLNAYLDD
                     YVFLIDAVLELQQVRFRAAELEFARQLAEVVLAHFADVENGGFYFTSDDHEQLIHRSK
                     TFSDDATPSGNGIAAFTLQRLGHLLGEPRYLAAAERTVRAAWPALEKYPVSHVTLVTA
                     LEELLQPPEIVILRGEGAALEGWRRELARMFVPRRLVLAVPADAAGLPAAL"
BASE COUNT          237 a          523 c          531 g          214 t
ORIGIN      
        1 cgagcgctgc cggtaatccg gccgcatcgg caggaaccgc cagcaccagc cgccgcggca
       61 cgaacatgcg cgcgagctcc cgccgccagc cctcgagcgc cgccccctca ccgcgcagga
      121 tgacgatctc gggcggttgc agcagctcct cgagcgcggt gaccagcgtc acgtgtgaca
      181 ccggatactt ctccagcgcc ggccacgccg cacgcacagt acgctcggcc gccgccagat
      241 agcgcggctc gccaagcagg tgcccgagac gctgcagggt gaaggccgcg atgccgttgc
      301 ccgagggggt ggcatcgtcg ctgaaggtct tggagcggtg gatcagctgc tcgtgatcgt
      361 cggaggtgaa gtagaagccg ccgttctcca catcggcgaa gtgcgcgagc accacctcgg
      421 ccagctgccg cgcaaactcc agctccgcgg cgcggaagcg cacctgctgc agctcgagca
      481 ccgcgtcgat gaggaagacg tagtcgtcga ggtaggcgtt gagatgcgcg cggccgtcct
      541 tgtaggtcgc gagcagccgg ccctcgcgcc agagcgtggc gcggacgaag tcgagagcgc
      601 ggctcgccgc ggcggccaac tgctcggcgg tgagcacgcg cgcagcgatc gccaggccgc
      661 ggatcatgag cgcgttccag gaggtgagga tcttctcatc gcgcgcgggg cgcacgcgct
      721 gcgcgcgcag cgccagcagc ttggcgcggg agctcgcgag cagcgcgctg acttcggcgg
      781 ccggccgctg cagactcgcg gcgatctgct ccacggtcgc atcgacgtac agattccagc
      841 ggccctcgaa gttggcttcg cgatcgaggc cgaagcgcgg cgcgaacgcg gcgagctctt
      901 cggcgctgag agcgcgccgg acctcctcac ggtcccacac gtagaacttg ccctcgtgcc
      961 cctcggagtc ggcgtcgagg ctcgagtaga agccgccctc gggggcttgc atctcgcgca
     1021 gcacccagcc ggcagtctcg agcgccacct gggtatagaa cgcatcggcg gtcccgagcg
     1081 aggagttggc gtacaccgcg agcagcgctg cgttgtcgta caacatcttc tcgaagtgcg
     1141 gaatcatcca gcgctcatcg accgagtagc ggcagaagcc cccgccgagc tgatcgcaga
     1201 tgccgccctc acccatgtga cgcagtgtga aacccgccat gtagagcgac tgcagcccgg
     1261 cctcatcgcc cgccgggctg ctgcgccagt cgcgcagcag ccgctcgatg gctttgggcg
     1321 ctggaaactt gggcgccccg ccgaagccgc cgtcgcggcg gtcgaaggtg ttcgcaagct
     1381 gcgcgcggca ggccttgagc ggcacgtcgg tgagcatggc gtcgggcgcg gccggcggcg
     1441 gattgattcc cgcgaaggcc tggagcagcg cttcgttctg cgcctgcagc tcggcgctgt
     1501 ggtcg
//
LOCUS       DATLKL010000526         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_17310, whole genome shotgun sequence.
ACCESSION   DATLKL010000526 DATLKL010000000
VERSION     DATLKL010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17310"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1412)
                     /locus_tag="VM713_07055"
                     /pseudo
     CDS             complement(51..1412)
                     /locus_tag="VM713_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(1428..>2084)
                     /locus_tag="VM713_07060"
     CDS             complement(1428..>2084)
                     /locus_tag="VM713_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84057.1"
                     /translation="ALGTVALVGVMAGVFALALAVLAAAGARAPALCALAALGVVAAA
                     AVALGFAVRAARAADFPRSLAWSYAGYAVALCLAARLIMPVVDRWQDLGSLAARIHSD
                     TAPRSLALLDPDETTIAMLDHGSATPLTILASGAAGPGDAVAGWFATGGAAARVLVLL
                     PGHASGPVTELLARLHPLPAPGDGIATSLTAAGAAALVARYELPQGRRYALLAPPAVH
                     "
BASE COUNT          395 a          695 c          765 g          229 t
ORIGIN      
        1 gggggcctca tcggcggcgg cgagcggctg ctgtagcggg cgcccccgcc tcaggcggtg
       61 cgcggcgcgc gcgtgccgta gtagccgagc gcgagcagtg cgaaccacac cggcgccacg
      121 tacaaggcga cgcgcgagtc cgggtcgcgc cacagcatga cggtcaccag cacgaggaac
      181 gcgagcacca ggtaattggc gagcggcgcc ccgggcatgc ggaacggcgc ggccgcggcg
      241 cggccggcgg ccaccgcgcg ccggtacctg gcgtgcgcca ccagaatgat tccccaggtt
      301 cacagcgtgc cgatgagcgc aatgctggtc accatggtga agacgcgctc gggcacgaga
      361 tagttgagca gcaccccgag catcatgacc gcggctgagg catggatccc cgccgccggc
      421 acctgcccgc ggttcagcgt ggcaaagacc cgcggggcct ggccgcgccg gccgagcgtc
      481 cacagcatgc gcccggtgct gaagagcccc gagttgcagg aggaggccgc cgcggtgatg
      541 accaccagat tgatgacgct cgccgcgccc gggagtccga gctggccgaa tacgaagacg
      601 aacggactca tcttcggatc gagctgcgtc cagggcacga gcgacatgat gatgagcagc
      661 gctcccagat aaaagatgag gatgcgggcg atgatgccgt tggtggcctt cggtaacacc
      721 accgccgggt cctgcgactc ccccgcggtg acgccgatga gctcgacgcc ctcgtaggcg
      781 aacatcacga tctgcagtgt gagcagcacc gccaggagtc cgccgggcag gaaaccgcca
      841 tgcgaccaga gattggagaa gcccgcggtg gcgccgaggg cgccgacatg gaacacgatc
      901 acgaccagac ccgcgacgat gagtccgacg atggtgacga ccttgatgag cgcgaaccag
      961 aactcgagct cgccaaacac gcgcacggcg aggaggttcg cgccgtagag gacggccagt
     1021 gcgatcagtg gcggcaacca ctgcggcacc tcggggagcc agaagcgcac gtagactccg
     1081 atcgcggtga tctcggccat gaccgtcgcg acccaggcga accagtagga ccagccggtg
     1141 atgaacccgg gcaggggccc gcagaactcg tccgcgtaag tcgcaaagga gccggctacc
     1201 ggccgatagg tgaggagctc accgagcgcc cgcatgatga aaaagatggc gacgccgcct
     1261 accgcgtacg cgagcaggag gccaggaccg gcgtgactga tcgccttggc ggagcctaga
     1321 aagagtccga caccgatcgt cccgccgatg gcgatcaact gaatgtggcg gttctgcagg
     1381 ctgcgggcga ggtcggttgc ggactgcgac atggaggctc ctgtctttca atgcaccgcc
     1441 ggcggcgcga gtagggcata gcgccggccc tgaggcaact cgtagcgcgc gacgagcgcg
     1501 gcggccccgg ctgcggtgag tgatgtggcg atgccatcgc cgggggcggg aaggggatgc
     1561 agccgcgcga ggagctcggt gaccgggccg ctcgcatgac ctggcaggag caccaatacc
     1621 cgcgccgctg cgccgcccgt cgcaaaccaa ccggcaacgg cgtcgcccgg gcccgcagcg
     1681 ccactggcga gaatcgtcaa gggagtggca gagccgtggt ccagcatggc aatggtggtc
     1741 tcatccgggt cgagcagcgc gagcgagcgc ggcgcggtgt cggagtggat gcgcgcggcg
     1801 agcgagccga gatcctgcca gcggtcgacg acgggcatga tgaggcgagc cgcgaggcac
     1861 agcgccaccg catagcccgc gtacgaccat gcgaggctgc gcgggaagtc cgcggcgcgc
     1921 gccgcacgca cggcgaagcc gagcgcgacc gcggccgccg cgacgacgcc gagcgcggcc
     1981 agcgcgcaaa gggccggcgc acgcgcgcct gcggcagcga gcacggcgag cgcgagcgcg
     2041 aagacgcccg ccatcacacc caccagcgcc accgtgccga gcgc
//
LOCUS       DATLKL010000527         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_74113, whole genome shotgun sequence.
ACCESSION   DATLKL010000527 DATLKL010000000
VERSION     DATLKL010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74113"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VM713_07065"
     CDS             <1..508
                     /locus_tag="VM713_07065"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVH84058.1"
                     /translation="TGAGFLLNNEMDDFSAKPGAANLYGVVGSEANAIAPGKRPLSTM
                     TPTILTDHGRVALVIGTPGGSRIATWVFQVITDWHDFHMPLAAAVAAPRVHHQLLPPN
                     TLFEEPYATLDPTVRAALEQRGYVLVNQGWNGDIEAIAVGSSGTFAVSDPRGRGVARV
                     LGAAATAR"
     gene            complement(520..>2051)
                     /locus_tag="VM713_07070"
     CDS             complement(520..>2051)
                     /locus_tag="VM713_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH84059.1"
                     /translation="AAACARVPLLGSVGVRRVINGPIPYAPDGNPLIGPARAARNFFH
                     CCAFTFGIAQAGGAGRVIAEWVVNGEPDWDVWPLDCRRYLAFADRSYALAKALETYQN
                     EYGVGYPQEERPAARPARTSPLYEALRRRGAVFGARGGWERAVYYGAAGDPAGPECSF
                     RRPHWQRAVERECAAVAQGVALLELPGFTRFEVSGSGAAAWLDHLIAGALPGVGRTVL
                     AYGCSPRGGIVMETTVTQMPDGRFWLLSAAAAERHDEDWLRRHYSRDYGPVDIANLTE
                     RFSTLVVTGPRARELLAHLTATDLSSAAFPWLAAGTLEIAGVPVTALRVSYVGELGYE
                     LHVAMEHLTHLHARLVEAGRELSLAPLGLYAVESLRLEKCYRSWKADLTTEYTPLAAS
                     LERFVRLDKAADFIGRDALRRIAAAGVPERFVPLVAEARAADAAAVSIVYGSGGQAVG
                     LVTSGGYGYRLKQSIALAYVRADLTAPGTELEVEILGERCRAVVGREPLYDSDNLRLR
                     A"
BASE COUNT          317 a          745 c          720 g          269 t
ORIGIN      
        1 gacgggcgcg gggttcctcc tcaacaacga gatggacgat ttctcggcca agccgggcgc
       61 ggccaacctt tacggagtcg taggcagcga ggccaacgcg atcgcgcccg gcaagcggcc
      121 gctgtcgacg atgacgccga cgattctgac cgaccacggg cgggtcgcgc tcgtcatcgg
      181 aacgcccggc ggctcgcgca tcgccacgtg ggtcttccag gtgataaccg actggcatga
      241 cttccacatg ccgctcgccg cagccgtcgc cgcgccccgc gtccaccacc agctgctccc
      301 gcccaacacg ctgttcgagg agccctatgc cacgctcgat ccgaccgtgc gcgcagccct
      361 cgagcagcgg ggctatgtgc tcgtgaacca aggctggaat ggcgacatcg aggcgatcgc
      421 ggtcggctcg tccggcacgt tcgccgtgtc cgacccccgc ggccggggcg tggcgcgcgt
      481 gctcggcgcc gccgccacgg cacgctgacg ctcccggtct cacgcccgta atcgcaggtt
      541 gtcggagtcg taaagtggct cgcgaccgac caccgcgcgg caccgctcgc cgaggatctc
      601 gacctcgagc tccgtgccgg gagcggtgag atcggcgcgc acataggcga gcgcgatgct
      661 ctgcttgagc cgatagccgt agccgcccga agtgacgagg ccgaccgcct gcccgccgga
      721 tccgtacacg atggagacgg ccgccgcgtc ggcagcgcgc gcctcggcga cgagcggcac
      781 gaagcgctcc ggcacgccgg ccgcggcgat cctgcgcaga gcgtcgcgac cgatgaagtc
      841 cgcagccttg tcgagccgca cgaagcgctc gagggaggcg gcgagcggcg tgtactcggt
      901 ggtgagatcc gccttccagc tgcgatagca cttctcgagc cgcaggctct cgaccgcgta
      961 gaggccgagg ggtgcgagtg agagctcgcg ccccgcctcc acgagccgcg catgcaggtg
     1021 tgtcagatgc tccatggcaa cgtgcagctc gtagccgagc tcgcccacgt agctcacccg
     1081 cagtgcggtg accgggacgc cggcgatttc gagcgtgcct gcggcgagcc agggaaaggc
     1141 ggcactggag agatccgtcg cggtgaggtg cgcgagcagc tcgcgcgcgc gcggtccggt
     1201 gacgaccagc gtgctgaagc gctcggtgag gttggcgatg tcgaccggtc cgtagtcccg
     1261 ggagtaatgc cttctcagcc agtcctcgtc gtgccgttcc gctgccgccg ccgacagcag
     1321 ccagaagcgc ccgtcgggca tctgcgtgac ggtcgtctcc atgacgatgc cgccgcgcgg
     1381 cgagcagccg taggcaagca ccgtgcggcc cacgcccggc agagcgcctg cgatcagatg
     1441 atccagccag gccgcggcgc cgctccctga cacctcgaac ctcgtaaagc ccgggagctc
     1501 gagcagcgcg accccctgcg cgaccgcggc gcactcgcgc tcgacggccc gctgccagtg
     1561 cgggcggcgg aaggagcact cggggcccgc cgggtcgccg gccgcgccgt agtacaccgc
     1621 gcgctcccag ccgccacgcg cgccgaacac cgcaccccgc cgccgcaagg cctcataaag
     1681 aggagaggtc cgcgccggcc gcgcggccgg gcgctcctcc tgcggatagc cgacgccgta
     1741 ttcgttctga tacgtctcta gggccttcgc cagcgcgtac gagcggtcgg cgaaggcgag
     1801 gtaccgccgg cagtcgaggg gccacacgtc ccagtccggc tcgccgttca ccacccattc
     1861 cgcgatcacc cggccggcgc cgccggcctg cgcgatgccg aaggtgaacg cacagcagtg
     1921 aaagaagttg cgcgcggcgc gcgcaggccc gatgagcggg ttgccgtccg gtgcgtaggg
     1981 gatcggccca ttgatgacac gtcggacgcc gacgctgccg aggagcggaa cgcgcgcgca
     2041 ggcggctgcg a
//
LOCUS       DATLKL010000528         1708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_62622, whole genome shotgun sequence.
ACCESSION   DATLKL010000528 DATLKL010000000
VERSION     DATLKL010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62622"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VM713_07075"
     CDS             complement(<1..693)
                     /locus_tag="VM713_07075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HVH84060.1"
                     /translation="MIATRLALPCAVLLLAVAPLAAQAAGTPIPAPPGVNARAWLLVD
                     YNTGKVLAQRRADDREEPASLTKLMTAYVVFQALSEGRLKLTDQVTISKHAWKAEGSR
                     TFVQVGTQIPADILIKGMIVQSGNDATIALAEKLGGTEDAFAQMMNEYARRLGMNESH
                     FINSDGLPAPEHYTSAHDMATLVSALIRQFPQYYGLFSLREFMWNNIRQGNRNTLLGK
                     DPSVDGLNTGHTD"
     gene            complement(735..1646)
                     /locus_tag="VM713_07080"
     CDS             complement(735..1646)
                     /locus_tag="VM713_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVH84061.1"
                     /translation="MTATLRPLRSARAAAAALLALILAACATTHRRPPAPAPFPVPPP
                     PGDIASIPDAVPRAEPRSAHGNPPFYDALGRRYFVLPSADGYDERGVASWYGPTLHGG
                     NTSSGELYDMYGMTAAHKTLPLPTYARVTNLVNGRSIVVRINDRGPFVANRIIDLSYT
                     AAAKLDMLREGTAWVEVRALSAGTPEPPPRIVPPPATSPPAQPPPAQPPPQPPLQPPP
                     AQAAAGPPLLYVQTGAFADPANAERELARLQAAGLASAFVMPPPAAGRSQLYRVRIGP
                     VGSVAEFDTLAARLTALGIPDALLVTD"
BASE COUNT          233 a          545 c          667 g          263 t
ORIGIN      
        1 gtccgtgtgt ccggtattca gtccgtcgac cgaggggtcc ttgccgagga gcgtattgcg
       61 gttgccctgg cggatgttgt tccacatgaa ctcgcgcagg ctgaacaggc cgtagtactg
      121 cgggaactgg cggatgagcg cgctgacgag cgtcgccatg tcgtgcgcgc tggtgtagtg
      181 ctccggggcc ggcagcccgt cgctgttgat gaaatggctc tcgttcatgc cgagccgccg
      241 cgcgtactcg ttcatcatct gcgcgaacgc gtcctcggtg ccgccgagct tctcggcgag
      301 cgcgatggtg gcatcgttgc ccgactgcac gatcatcccc ttgatgagga tgtcggcggg
      361 gatctgcgtg ccgacctgca cgaaggtccg cgagccttcc gccttccagg cgtgcttgct
      421 gatggtcacc tggtcggtga gcttgaggcg cccctcgctg agtgcctgga agaccacgta
      481 ggcggtcatg agcttggtga gactcgccgg ctcttcacga tcgtcggcgc ggcgctgcgc
      541 gagcactttg ccggtgttgt aatcgacgag gagccaggcg cgggcattga cgcccggcgg
      601 cgccgggatc ggcgtgccgg cggcctgcgc cgcgagcggc gcgaccgcca gcagcagcac
      661 ggcgcagggc agggcaagac gcgtagcgat caccgtgagg attccttccg ttgcgcagag
      721 ccgcaaagtc tagctcagtc agtcaccaga agggcgtcgg ggatgccgag cgcggtgagg
      781 cgcgcggcga gcgtgtcgaa ctcggcgacg ctgccgaccg gcccaatgcg cacgcggtag
      841 agctgcgagc ggcccgccgc aggcggcggc atcacgaagg cgctcgcgag gcccgcggcc
      901 tgcagccgcg cgagctcgcg ctcggcattg gccggatcgg cgaaggcgcc ggtctgcacg
      961 tacagcaagg gcggcccggc cgcggcttgc gccggcggcg gttggagcgg cggctgcgga
     1021 ggcggttgcg ctggcggtgg ctgcgccgga ggcgatgtcg ctggcggcgg cacgatgcgg
     1081 ggcggcggct cgggcgtccc cgcgctcagc gcccgcacct ccacccacgc ggtcccttca
     1141 cgcagcatgt cgagcttcgc cgcggcggtg taggagaggt cgatgatgcg gttggcgacg
     1201 aacggcccgc gatcgttgat gcgcaccacg atgctcctgc cgttgacgag gttggtgacg
     1261 cgcgcgtagg tcggcagcgg cagcgtcttg tgcgcggccg tcatgccgta catgtcgtaa
     1321 agctcgccgc tcgaggtatt gccgccgtga agggttgggc cataccagga cgcgacaccg
     1381 cgctcgtcat agccgtccgc ggaaggcagc acgaaataac gccgcccgag cgcatcgtag
     1441 aagggcgggt tgccgtgcgc gctgcgcggc tcggcgcgcg gcacggcgtc ggggatgctc
     1501 gcgatgtcgc cgggcggcgg cggcaccggg aacggcgccg gcgcgggcgg acgtcggtgg
     1561 gtcgtggcgc aggccgcgag gatgagcgcg agaagtgccg cggcggcggc gcgcgcggac
     1621 cgcagcggcc gaagcgtcgc ggtcacggct gctcggcgtg cacgcgcgcc gccagcgtgg
     1681 tggcgaggtc gtacacggtc atggcgta
//
LOCUS       DATLKL010000529         4644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_141709, whole genome shotgun sequence.
ACCESSION   DATLKL010000529 DATLKL010000000
VERSION     DATLKL010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141709"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VM713_07085"
     CDS             complement(<1..491)
                     /locus_tag="VM713_07085"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84062.1"
                     /translation="MLRRLAALDPARYPLLLDSAAAGPLGTTTVLLAAPRAALWLGAD
                     GRLGADGTAIDGDTFLAALESWWRREGAAGNPEEMSAQLPFTGGWALYLGYELAQEIE
                     PRLALPRTPLPWVAYALRTPGALIHELGSGRVLAVCEAGSGDTLELIVADAYAAAAQA
                     DPP"
     gene            complement(565..1809)
                     /gene="fabF"
                     /locus_tag="VM713_07090"
     CDS             complement(565..1809)
                     /gene="fabF"
                     /locus_tag="VM713_07090"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012149092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HVH84063.1"
                     /translation="MSKRRVVVTGLGLVSPVGSTVASAWDAILRGESGIGPLTRFDVS
                     AFPVRFGGQVRDFDVSQYLQPKEARRMDEFMHYGVAAGVQAVTDSGIDFSKTDPTRCG
                     AVCGAGIGGLWTIETEYGEYLKAQSPRKISPFFVPATIINMIAGHLSIRYGLKGPNLG
                     VVTACTTSTHAIGLGMRTIQYGDADVMVAGGGEMATTRCGLGSFGQAKALSTRNDAPA
                     EASRPWDKDRDGFVLSDGGGAVLLEELEYARRRGAHIYAELVGFGMSGDAHHITAPPE
                     DGEGARIAMVNALRDAGLNPEQVQYINAHATSTPLGDKSETVALKRAFGEAVRRIAVS
                     STKSMTGHLLGAAGVIEAIFSVLAIRDGVAPPTINYRTPDPECDLDYVPNTARRMKID
                     VSLSNSFGFGGTNGSVVFRRFG"
     gene            complement(1838..2080)
                     /gene="acpP"
                     /locus_tag="VM713_07095"
     CDS             complement(1838..2080)
                     /gene="acpP"
                     /locus_tag="VM713_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HVH84064.1"
                     /translation="MSTVEQQVKAIVAEQLGVKQEQVTNDASFVDDLGADSLDTVELV
                     MALEEEFEIEIPDEDAEKITTVHQAIDYINERRAKS"
     gene            complement(2289..3029)
                     /gene="fabG"
                     /locus_tag="VM713_07100"
     CDS             complement(2289..3029)
                     /gene="fabG"
                     /locus_tag="VM713_07100"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HVH84065.1"
                     /translation="MLEKEIALVTGASRGIGHAIALALAKAGARVIGTATSPEGVAKL
                     TTTLASHGCNGRGALLDAGDGASIDALVAGLEAAGELPTILVNNAAITRDTLLLRMKQ
                     EDWDRVIATNLTSVFRLSKACIRRMVKERRGRIVNLTSVVGLTGNPGQANYAAAKAGL
                     LGFTKSLAQELASRGITVNAVAPGFIDTDMTRALTEEQRAALMTRVPLGRLGTPEDIA
                     AAVLFLASPQAAYITGETLHVNGGMYMA"
     gene            complement(3022..3987)
                     /gene="fabD"
                     /locus_tag="VM713_07105"
     CDS             complement(3022..3987)
                     /gene="fabD"
                     /locus_tag="VM713_07105"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HVH84066.1"
                     /translation="MAFAFVFPGQGSQSVGMLGALAAAEPTVRATFDEASAVLGYDLW
                     KLVSEGPEEELNSTARTQPALLTAGVATWRCWRARGGAEPATVSGHSLGEFTALTCAE
                     SLEFRAAVGLVRFRGEVMQEAVPAGAGAMAAILGLEDAAVEAACREAATGGGAGAVVE
                     AVNFNSPGQVVIAGESGAVARAIAAAKARGAKRAVLLPVSVPSHSTLMRGAAERLRGR
                     LSAVTLKPPRLRYVSAVDAAAHSEPAEIRDLLVRQLSSPVRWTDTVRALIDTGVEEII
                     ECGPGRVLTGLSRRIERRAGLEFLALEDPAALDAALTATREGNHA"
     gene            complement(4019..4615)
                     /gene="rrtA"
                     /locus_tag="VM713_07110"
     CDS             complement(4019..4615)
                     /gene="rrtA"
                     /locus_tag="VM713_07110"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HVH84067.1"
                     /translation="MNRAVRTLRALNCDTRYGWTLLAVCALLVLITLSGETGRTWLRY
                     DRAALGCGELWRLVTAHLVHLDLHHALLNCLGLLLMWALFARDYTPRQWLIIVLGSIG
                     VIDAGLALWDSTLRWYVGSSGALHGVMAAGTLAHLRRRERDGWLLAAFLAGKLLWEQA
                     FGALPLSGSDPVVVDAHLFGAAGGLAAAAFLRGGVERL"
BASE COUNT          705 a         1629 c         1607 g          703 t
ORIGIN      
        1 tcgggaggat cggcctgggc ggcggccgcg taggcatcgg caacgatgag ctcgagcgtg
       61 tcgcccgagc ccgcctcgca gaccgcgagc acccggccgc tgccgagctc gtggatcagt
      121 gcacccggag tgcgcagcgc atacgccacc caggggagcg gggtgcgcgg cagtgcgagc
      181 cgcggctcga tctcctgcgc gagctcgtag ccgagataga gcgcccagcc ccccgtgaac
      241 ggcaactgcg cgctcatctc ctcaggattc cctgctgcgc cctcccggcg ccaccagctc
      301 tcgagcgcgg cgagaaaggt gtcgccgtcg atcgcggtgc cgtcggcgcc gagccggcca
      361 tcggccccga gccagagcgc cgcgcgcggt gcggcgagca gcacggtcgt ggtgccgagc
      421 ggacccgccg ccgcgctatc gagcagcagc ggatagcgcg cgggatcgag cgccgcgagg
      481 cggcgcagca ccgccggcgg taccttgagc tcgcgaacga gaggtgcgtg cgtaaccgcg
      541 ggcccgcgag gcgctgcgcc gtccctagcc gaagcgccgg aagacgaccg agccgttggt
      601 accgccgaag ccgaaggagt tcgacaggct cacgtcgatc ttcatgcgcc gcgcggtgtt
      661 cgggacgtag tccaggtcgc actccgggtc gggcgtgcgg tagttgatgg tgggcggagc
      721 gaccccgtcg cggatcgcca gcaccgagaa gatcgcctcg atcactccgg cggccccgag
      781 caggtgcccg gtcatcgact tggtcgagct gacggcgatg cggcgcaccg cttcgccgaa
      841 ggcgcgcttc agcgcgacgg tttccgactt gtcaccgagc ggcgtcgagg tcgcatgggc
      901 gttgatgtac tgcacctgct cgggattcaa gcccgcgtcg cgcagcgcat tcaccatcgc
      961 gatgcgtgcg ccctccccgt cctccggggg cgcggtgatg tgatgagcgt caccgctcat
     1021 gccgaagccg acgagctcgg cgtagatgtg cgcgccgcgg cggcgcgcgt actcgagctc
     1081 ctcgagcagc accgcgcctc cgccatcgct cagcacgaag ccgtcgcggt ccttgtccca
     1141 ggggcggctc gcctcggcgg gggcgtcgtt gcgcgtcgag agcgccttgg cctggccaaa
     1201 gctgccgagc ccgcaacgcg tcgtcgccat ctcgccgccg ccggccacca tcacgtcggc
     1261 atcgccgtac tggatggtgc gcatgccgag gccgatcgcg tgcgtcgagg tggtgcaggc
     1321 ggtcactacg ccgaggttcg ggcccttgag gccgtagcgg atcgagaggt gccccgcgat
     1381 catgttgatg atcgtggcgg ggacgaagaa tggggagatc ttgcggggac tctgcgcctt
     1441 gaggtattcg ccgtattcgg tctcgatcgt ccacagtccg ccgatgccgg cgccgcagac
     1501 cgcgccgcag cgggtcggat cggtcttgct gaaatcgatg ccggaatcgg tgacggcctg
     1561 cacgccggcg gcgacgccgt agtgcatgaa ctcgtccatg cggcgcgcct ccttcggctg
     1621 cagatactgg ctgacgtcga agtcgcgcac ctgcccgccg aagcgcaccg ggaacgccga
     1681 gacatcgaag cgcgtcagcg ggccgatgcc gctctcaccg cgcaggatgg cgtcccatgc
     1741 cgaggcgacc gtgctcccga ccggagacac cagtccgaga cccgtcacga cgacgcggcg
     1801 cttgctcaca cgattcctgt atggcgcgcg cggtgggtca ggacttggcg cggcgctcgt
     1861 tgatgtagtc gatggcctgg tggacggtgg tgatcttctc ggcgtcctcg tccgggattt
     1921 cgatctcgaa ctcctcctcg agggccatca ccagctcgac ggtgtcgagc gagtcggctc
     1981 cgagatcgtc gacgaacgaa gcgtcgttcg tgacctgttc ctgtttgacg cccagctgtt
     2041 cggcaacgat ggctttgacc tgctgctcga ctgtgctcat tagcggttgg tcctctgtag
     2101 gcttgcttcc cgggcttggg acttggtccg ccggcaggct acagccggac agcgggcgcg
     2161 gtattgtagg ggaacacccc ccagggtgcc acttgcgcga gtggttgttt ttactggcga
     2221 aagtcccttt gagccgccct ccgataccgg gccgacggac ctgggttgtg gcgtgcagcc
     2281 aacgcctctc aggccatgta catcccgccg ttgacgtgca aggtctcgcc ggtgatgtag
     2341 gcggcctgcg gagaggcgag aaacagcacc gcggcggcga tgtcctcggg cgttccgagc
     2401 cgcccgagcg gcacccgcgt catgagggcg gcgcgctgct cctcggtgag tgcgcgcgtc
     2461 atgtcggtgt cgatgaagcc gggcgcgacc gcgttcaccg tgatgccgcg cgaggcgagc
     2521 tcctgggcca gcgacttggt gaagccgagc agcccggcct tcgcggccgc gtaattcgcc
     2581 tgtccgggat tgccggtgag gcccaccacc gaggttagat tgacgatgcg gccgcgccgc
     2641 tccttgacca tccgccttat gcaggccttc gacagacgga ataccgaggt gagattggtt
     2701 gcgatcacgc ggtcccaatc ctcctgcttc atgcgcagca gcagcgtgtc gcgggtgatc
     2761 gccgcattgt tgacgaggat cgtcggcagc tcgcccgcgg cctcgagccc ggcgactagg
     2821 gcatcgatcg aggcgccgtc gcccgcatcg agcagcgctc cgcgcccgtt gcatccgtgc
     2881 gacgcgagtg tcgtggtgag tttcgccaca ccctcggggc tggttgcggt gcctatcacg
     2941 cgtgctccag ccttcgccag cgcgagcgcg atcgcgtgtc cgatcccgcg cgaggcgccg
     3001 gtcacgagcg cgatttcctt ctcaagcatg gttgccctcc cgggttgccg tgagcgccgc
     3061 atccagcgcg gccggatcct ccagcgcgag aaactcgagc cccgcgcggc gctcgatgcg
     3121 gcggctgagc ccggtgagca cgcgccccgg gccgcactcg atgatttcct cgacccccgt
     3181 gtcgatgagc gcgcgcaccg tgtcggtcca gcgcaccggg ctcgacagct gccgcaccag
     3241 gaggtcgcgg atctcggccg gctcggagtg cgccgccgca tcgaccgcgc tcacataacg
     3301 caggcgtggc ggcttgagtg tcaccgccga caaccgcccg cgcagccgct cggcggcgcc
     3361 gcgcatgaga gtgctgtggg agggcacgct caccggaagc aggaccgcgc gcttggcgcc
     3421 gcgcgccttg gctgcggcga tggcgcgcgc gaccgcaccg ctctcaccgg cgatcaccac
     3481 ctgtcccggc gaattgaaat tcacggcctc caccaccgct ccggcgccgc ctccggtagc
     3541 cgcctcgcgg cacgccgcct ccaccgccgc gtcctcgagc ccgaggatcg cagccatggc
     3601 gccggcgccg gccggaacgg cctcctgcat cacctcaccg cggaagcgca ccagtcccac
     3661 cgcggcgcgg aactcgaggc tctcggcgca agtgagcgcg gtgaactccc cgaggctgtg
     3721 accggagacg gtcgcaggct ccgcgccgcc gcgcgcccgc cagcagcgcc aggtggcgac
     3781 acccgcggtc agcagcgccg gctgggtgcg tgcggtcgag ttgagctctt cctcgggacc
     3841 ttcactgacg agcttccaca ggtcgtagcc gagcaccgcc gaggcctcat cgaaggtggc
     3901 gcgcacggtc ggctcggccg cggccagcgc cccgagcatt ccgaccgact gcgagccctg
     3961 tccgggaaag acgaacgcaa acgccatggc gcccccgtgc cgagtgtgac tgcgaaaatt
     4021 ataatcgctc gaccccaccc ctcaggaatg ccgcggctgc gagcccgccc gccgcgccga
     4081 acaggtgcgc atcgacgacc accggatcgc tgccggagag tggcagcgcg ccgaacgcct
     4141 gttcccagag gagcttaccg gcgaggaacg ccgccagcag ccagccgtcg cgctcgcggc
     4201 ggcgcaggtg cgcgagcgta cccgcagcca tgacgccatg cagtgctccc gaggaaccga
     4261 cgtaccagcg cagagtcgaa tcccacagcg ccagccccgc gtcgatcacg ccaatcgagc
     4321 cgagcacgat gatcagccac tgccgtggcg tgtaatcacg ggcgaacagg gcccacatca
     4381 acaggagtcc cagacagttg agcagggcgt ggtgcaggtc gagatgaacc agatgcgccg
     4441 tcacgagccg ccagagctcg ccgcagccga gcgcggcgcg gtcgtagcgc agccaggtgc
     4501 gcccggtctc cccgctgagg gtgatgagga cgagcagcgc gcacaccgca agcagggtcc
     4561 agccgtagcg ggtgtcgcaa ttcagtgcgc ggagcgtccg gacggcgcga ttcacaggga
     4621 acgctcaccc cttttgttat gatc
//
LOCUS       DATLKL010000530         4292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_130481, whole genome shotgun sequence.
ACCESSION   DATLKL010000530 DATLKL010000000
VERSION     DATLKL010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130481"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VM713_07115"
     CDS             <1..1134
                     /locus_tag="VM713_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVH84068.1"
                     /translation="DEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFEGNEGIIVI
                     AATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREQILRVHMRRVPLADDVKPALIARG
                     TPGFSGADLANLVNEAALFAARANKRIVGMDEFEKAKDKIMMGAERRSMVMSEAEKKM
                     TAYHEAGHAIVGMTVPEHDPVYKVTIIPRGRALGVTQFLPEQDRYSFSKRRLESAIAT
                     LFGGRIAEELIFGPESVTTGASNDIERATELARNMVTKWGLSDKLGPLTYSEETGEVF
                     LGRSVTQHKQVSDVTAHAIDEEIRRVIEVNYKRAREVLTSSLDKLHTMADALMKYETI
                     DEEQLRDLMAGKVPKPPAGWDETLSNKPPKAEPPSGAIPAGQH"
     gene            1372..2217
                     /gene="folP"
                     /locus_tag="VM713_07120"
     CDS             1372..2217
                     /gene="folP"
                     /locus_tag="VM713_07120"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HVH84069.1"
                     /translation="MPSQPVLLRCRDKTVDLTHPVVMGVLNVTPDSFSDGGRYFDTET
                     AVRHGVRLAEEGAAFIDVGGESTRPGAAPVDMEEELRRVIPVVERLHGATGAVISVDT
                     SKPEVMRAAAAAGAGLINDVRALREPGALEAARASGCAVCLMHMQGEPRTMQAAPSYQ
                     DVVVEVRAFLAERAQACRAAGFSADRLVLDPGFGFGKTLEHNLTLLRHLRDIGAGGLP
                     LMVGLSRKSMVGALTGRPPDERVQGSVALALIAALNGARILRVHDVAPTVDALKVLAA
                     VGRAA"
     gene            2313..3647
                     /gene="glmM"
                     /locus_tag="VM713_07125"
     CDS             2313..3647
                     /gene="glmM"
                     /locus_tag="VM713_07125"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004639966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HVH84070.1"
                     /translation="MGRRYFGTDGVRGRVGEHPMTVDFALHLASSAARVLAPDGGTVL
                     IGKDTRLSGYMFESALEAGFVAAGVDVMLIGPLPTPGIAYLTTRFDCDFGVVISASHN
                     LYDDNGIKFFDGSGAKLSDELEESIESELAQPPLTRTSRSLGRATRVDRSRTVYQEFC
                     ASTMPAGIDLAGLKIVVDCANGASYKVAPRILADLGAEIVPIGCSPNGRNINDGCGST
                     VPDLLQLTVPGVRANVGIALDGDGDRLVMVDHLGRIVDGDQLLYVIARALKQAGTLRG
                     PVVGTVMSNLGLEVGLRASGIEFRRAAVGDRYVLAMLRELQGTLGGETSGHILCLDKT
                     TTGDGLVCALQVLAVMQQTGRPLAELASGMQKYPQVLLNVKVAKRFDPSMVPAVQEAM
                     ARIERTMGGEGRVVLRPSGTEPVIRVMVEGRDEGATRAAATELADVVRAAAG"
     gene            3747..>4292
                     /gene="tpiA"
                     /locus_tag="VM713_07130"
     CDS             3747..>4292
                     /gene="tpiA"
                     /locus_tag="VM713_07130"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HVH84071.1"
                     /translation="MRRPIVAGNWKMHGSRADNARLAEELLAQAPASAAADCVVCPPL
                     VYLQEVGRLLRDSLFILGAQDVCADPQGAFTGEVSAAMLKDIGCEYVIVGHSERRLLY
                     RESDQLVARKFSAAHAKGLVPILCVGEQLADREAGRTREIVARQLDVVLELCGAGTLE
                     RGVIAYEPVWAIGTGRNATPDQ"
BASE COUNT          676 a         1446 c         1510 g          660 t
ORIGIN      
        1 gacgagatcg acgccgtcgg ccggcaccgt ggcgcggggc tgggcggcgg gcatgacgag
       61 cgcgagcaga cgctcaacca gctgctggtc gagatggacg gcttcgaagg caacgagggt
      121 atcatcgtca tcgccgccac caaccgtccg gacgtgcttg acccggcgct gctgcgcccg
      181 ggccggttcg accgccaggt ggtcgtgccg ctgcctgacg tgcgcggccg cgagcagatc
      241 ctgcgcgtgc acatgcggcg tgtgccgctc gccgacgacg tcaagccggc gctcatcgcg
      301 cgcggcaccc ccggattctc gggcgcggat ctcgccaacc tggtgaacga ggcggcactg
      361 ttcgccgcgc gtgccaacaa gcgcatcgtc ggcatggacg agttcgagaa agccaaggac
      421 aagatcatga tgggcgccga gcggcgctcc atggtgatga gcgaagccga gaagaagatg
      481 acggcctatc acgaggccgg ccatgcgatc gtcggcatga cggtgccgga gcacgatccc
      541 gtgtacaagg tgacgatcat cccacgcggc cgggcgctcg gcgtcacgca gttccttccc
      601 gagcaggatc gctacagctt ctccaagcgc cgcctcgaga gcgccatcgc gacgctgttc
      661 ggcggccgga tcgccgaaga gctgatcttc ggccccgagt cggtgaccac cggcgcctcc
      721 aacgacatcg agcgggccac cgagctggcg cgcaacatgg tcaccaagtg gggtctgtcg
      781 gacaagctcg ggccgctcac gtattcagaa gagaccggcg aggttttcct cggccgcagc
      841 gtcacccagc acaagcaggt atcggacgtt accgctcacg cgatcgacga ggaaattcgg
      901 cgcgtgatcg aggtcaacta caagcgcgcg cgcgaggtcc tcacgagctc gctcgacaag
      961 ctccacacga tggccgacgc gctcatgaag tacgagacca tcgacgagga gcagctgcgg
     1021 gacctcatgg cgggcaaggt gccgaagccg ccggcgggct gggacgagac gctgtcgaac
     1081 aagccgccga aggcggagcc gccgagcggc gcgataccgg cgggacagca ctgagccacc
     1141 tctgttgtcg agaccgcgcc ccgttgtgaa ataagcattt ccgggaacgc cgtgaatcca
     1201 ttgcgctgcg cagtccctgc tgcgcgcaag ccctgggcgg ccatccctgg ccgcccgctc
     1261 gccgcatgcg gcgagcccct gtaggctgcg ggcgcggcct ccctgccgcg cacgctcccg
     1321 gaagtgcata tttcacaccg ggtcgcaaca caactctccg ccgcgactca gatgccatcc
     1381 cagccggtat tgctcaggtg cagagataag acggtcgacc tgacccatcc ggtcgtgatg
     1441 ggtgtgctca atgtgacgcc ggattccttc tccgacggcg gccgctactt cgacaccgaa
     1501 acggcggtcc ggcacggcgt gcgcctggcg gaggagggcg cggccttcat cgacgtgggc
     1561 ggcgagtcga cacggccggg cgctgcaccg gtcgacatgg aggaggagct gcgccgcgtc
     1621 atccccgtcg tcgagcggct gcacggcgcc accggcgccg tgatttccgt cgataccagc
     1681 aagcccgagg tcatgcgtgc cgcagcggcg gccggcgccg gcctcatcaa cgacgtgcgc
     1741 gcgctgcgcg agcccggggc gctcgaggcg gcgcgcgcaa gcggctgcgc cgtgtgcctg
     1801 atgcacatgc agggcgagcc ccgcaccatg caggccgctc ccagctatca ggatgtcgtg
     1861 gtcgaggtgc gggcctttct cgcagagcgc gcccaggctt gccgcgcagc cggcttttcc
     1921 gccgaccgcc tggtcctcga ccccggcttc ggcttcggca agactctcga gcacaatctg
     1981 acactgctgc gccacctgcg cgatatcggt gccggcggac tgccgctaat ggtcgggttg
     2041 tcgcgcaagt ccatggtggg cgcgctcacg ggccggccgc cggatgagcg ggtgcagggc
     2101 agcgtcgcgc tcgccctgat cgcggcgctg aacggcgcgc gaatcctgcg cgtgcatgac
     2161 gtcgcgccga cggtcgatgc actgaaggtc ctggcggccg tcgggcgagc ggcctgagct
     2221 tgatcgaggt cacaagcggc aacgccgcgg tggcccgcga gcgatgcggc ggcgataatg
     2281 ccgcgctgct gttgcagcgc tgaggttgtg ctttgggcag gagatacttc ggaaccgatg
     2341 gcgtccgtgg ccgggtgggc gagcacccga tgacggtcga ctttgcgctg cacctcgcga
     2401 gctctgcggc gcgcgtgctc gcgcctgacg gcggcacggt gctgatcggt aaggacacgc
     2461 gcctctcggg ctacatgttc gagtcggcgc tggaagccgg cttcgtcgcc gccggcgtcg
     2521 atgtcatgct catcgggccg ctgccgacac ccggcatcgc ctatctgacg acgcgcttcg
     2581 attgtgattt cggcgtggtg atcagcgcct cccacaatct ctacgacgac aacggcatca
     2641 agttcttcga tggctcgggg gccaagctct ccgacgagct ggaggagtcg atcgagagcg
     2701 agctggctca gccgccgctc acgcgcacct cacgcagcct cgggcgtgcc acgcgcgtcg
     2761 accgttcgcg caccgtgtat caggagttct gcgcctcgac catgccggcc ggaatcgacc
     2821 tcgcggggct caagatcgtg gtcgactgcg ccaacggcgc ttcctacaag gtggccccgc
     2881 gtatcctcgc cgatctcggc gccgagatcg ttccgatcgg ctgctcgccc aacggccgca
     2941 acatcaacga cggttgcgga tcgaccgtac cggacctgct gcagctcacc gtgcccgggg
     3001 tgcgcgccaa cgtggggatc gcgctcgacg gcgacggcga tcggctggtg atggtcgatc
     3061 acctgggtcg catcgtcgac ggcgatcagc tgctgtacgt catcgcgcgc gcgctcaagc
     3121 aggcaggcac gctgcgcggc ccggtggtcg gcacggtgat gagcaacttg gggctcgagg
     3181 tggggctgcg cgcgagcggc atcgagttcc ggcgcgccgc cgtcggtgac cgctatgtgc
     3241 tcgccatgct gcgcgagctg caggggactc tcggcggcga gacctccgga catatcctgt
     3301 gcctcgacaa gaccacgacc ggcgacgggc tggtgtgcgc gctgcaggtg ctggcggtga
     3361 tgcagcagac cggccgcccg ctcgccgagc tcgcctcggg catgcagaag tacccgcagg
     3421 tgctcctcaa tgtaaaggtg gcgaagcgct tcgacccttc gatggtgccg gcggttcagg
     3481 aggccatggc gcgcatcgag cgcaccatgg gaggcgaagg gcgcgtagtg ctgcggcctt
     3541 cgggcaccga gccggtgatc cgcgtcatgg tcgaggggcg cgatgagggc gcgacccgag
     3601 cggcagcaac cgagctcgcc gacgtcgtca gggcagcggc cggctgatct cgccggagcg
     3661 ttgcgccacc ccccctcgat cgctatcatc ccgcgcccct ccgggggtag gccccctctc
     3721 agaagcgagt ccaggacttg aatctcatgc gccgcccgat cgttgccggg aactggaaga
     3781 tgcacggctc ccgggccgat aacgcccggc tggccgagga gctgctggcc caggcgccgg
     3841 cctcggcagc ggctgactgc gtcgtctgcc cgccgctcgt gtatctgcag gaggtcgggc
     3901 ggctgctgcg cgactcgctg ttcatcctcg gagcccagga cgtgtgcgcg gacccacagg
     3961 gcgcgttcac cggcgaggtc tcggccgcga tgctcaagga catcggctgc gagtacgtga
     4021 tcgtcggtca ctcggaacgg cgactgctct atcgggagtc agaccagctg gtggcacgca
     4081 agttttcagc ggcgcacgcc aaggggctcg ttccgatcct gtgcgtgggc gaacagctcg
     4141 ccgatcgcga ggcgggacgt acgcgcgaga tcgtggcgcg gcagctcgac gtggtgctgg
     4201 agctgtgcgg cgctggaacg ctggaacgcg gcgtgattgc ctacgagccg gtgtgggcga
     4261 tcggcaccgg ccgcaacgcc acgcccgacc ag
//
LOCUS       DATLKL010000531         2071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_74154, whole genome shotgun sequence.
ACCESSION   DATLKL010000531 DATLKL010000000
VERSION     DATLKL010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74154"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /gene="rpoB"
                     /locus_tag="VM713_07135"
     CDS             <1..1250
                     /gene="rpoB"
                     /locus_tag="VM713_07135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729307.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HVH84072.1"
                     /translation="DERALAIERAEIERLAKDRDDERVILDRSFYARLKSLLMNRIVV
                     RGPRTVKAGSKVSESLLSELTQGQWRQIIVKNDEIMSAIEQLSRQFDQSIAALQKRFE
                     DKVEKLQRGDELPPGVMKMVKVFVAVKRKLQPGDKMAGRHGNKGVISKIVPMEDMPYL
                     EDGTPVDIVLNPLGVPSRMNVGQILETHLGWACHGLGRQVAQLLEKVQHSGKIGEVRR
                     WLERAYGKTQKEELAELSDDEVLELASNLRAGLPVSSPVFDGAREDDIVHMLESAGLE
                     RSGQVTLVDGRTGDPFERKVTVGYIYMLKLHHLVDDKIHARSIGPYSLVAQQPLGGKA
                     QFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVAGRTRVYEAIVKGDDNFEAGIPE
                     SFNVLVKELRSLGLNVELAQSGY"
     gene            1361..>2071
                     /locus_tag="VM713_07140"
     CDS             1361..>2071
                     /locus_tag="VM713_07140"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004666357.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HVH84073.1"
                     /translation="MNELLKIFGQVASPPTFDQIKISIASPERIRSWSYGEIKKPETI
                     NYRTFKPERDGLFCARIFGPIKDYECLCGKYKRMKYRGIICEKCGVEVTLSKVRRERM
                     GHIELASPVAHIWFLKSLPSRIGLLLDMTLRDLERVLYFETHVVVEPGLTALKRHQLL
                     TEDQYQNAIDEFGEQSFEAKIGAEALKEMLARIDLEKEREALRAELKETSSEAKRKKY
                     VKRLKLVESFVDSGSRPEW"
BASE COUNT          398 a          639 c          709 g          325 t
ORIGIN      
        1 aggacgagcg cgcgctcgcc atcgaacgcg ccgagatcga gcgcctcgcc aaggatcgtg
       61 acgacgagcg ggtgatcctt gatcgcagct tctacgcgcg gctcaagagc ctgctcatga
      121 accgcatcgt ggtgcgtggc ccgcggacgg tgaaggcggg ctcgaaggtg agcgagtccc
      181 tgctctccga gctgacccaa ggccagtggc gccagatcat cgtcaagaac gacgagatca
      241 tgtcggcgat cgagcagctc tcgcgccagt tcgaccagtc gatcgcggcg ctgcagaagc
      301 gcttcgagga caaggtcgag aagctgcagc gcggcgacga actgccgccc ggcgtcatga
      361 agatggtgaa ggtcttcgtc gccgtgaagc gcaagctgca gcccggcgac aagatggccg
      421 gacggcacgg caacaagggc gtcatctcca agatcgtccc catggaggac atgccctatc
      481 tggaggacgg cacgcctgtc gacatcgtgc tcaatcccct gggcgtgccc tcgcgcatga
      541 acgtgggcca gatcctcgag acccatctcg gctgggcctg tcacgggctg ggccgccagg
      601 tggcgcaact cctggagaag gtgcagcact cgggcaagat cggcgaggtc cgccgctggc
      661 tcgagcgggc ctacgggaag acccagaagg aggaacttgc cgagctctcg gacgacgagg
      721 tgctcgagct tgcgagcaac ctcagggcgg gcctccccgt ctcctcgccc gtattcgacg
      781 gcgcgcggga ggacgacatc gtccatatgc tcgagagcgc gggactcgag cgctccggcc
      841 aggtgacgct ggtggacggc cgaaccggcg acccgttcga gcgcaaggtg acggtcggct
      901 acatctacat gttgaagctg caccatctgg tcgacgacaa gatccacgcg cgctcgatcg
      961 ggccctacag cctcgtggcc cagcagccgc tgggcggcaa ggcgcaattc ggtgggcagc
     1021 gcttcggcga gatggaggtg tgggcgctcg aggcctatgg cgcggcgtac acgctgcagg
     1081 agatgctcac ggtcaagtcg gacgacgtgg ccggtcgcac gcgcgtctac gaggcgatcg
     1141 tcaagggcga cgacaatttc gaggcgggca ttccggagtc cttcaacgtg ctggtcaagg
     1201 agctccggtc gctcggcctc aatgtcgagc tcgcccagtc gggctattga gcggggccgg
     1261 cagcgcggtt gctgggtagg ccacgtggga tggatgagtt gagacactgt cgccggcgcc
     1321 ttcgagaggc ttgccggccg agcagagcgg gagagttggc atgaacgagc tgttgaagat
     1381 cttcggccag gtcgcgagtc cgccgacctt cgaccagatc aagatttcga tcgccagccc
     1441 ggagcgcatc cgctcctggt cctacggcga gatcaagaag ccggaaacca tcaactaccg
     1501 gaccttcaag ccggagcgcg acgggctgtt ctgcgcccgc atcttcggcc cgatcaagga
     1561 ctacgagtgc ctgtgcggca agtacaagcg catgaagtat cgcggcatca tctgcgagaa
     1621 gtgcggcgtc gaggtgacgc tctccaaggt ccggcgcgag cgcatgggcc acatcgagct
     1681 cgcctcgccc gtggcgcaca tctggttcct gaagtcgctg ccgagccgga tcggcctgtt
     1741 gctggacatg acgctgcgcg acctcgagcg cgttctctat ttcgagaccc atgtggtggt
     1801 cgagccgggg ctgaccgcgc tcaagcgcca ccagctgctc accgaagacc aatatcagaa
     1861 cgcgatcgac gagttcggcg agcaatcctt cgaggccaag atcggcgccg aggcgctcaa
     1921 ggagatgctg gcccgaatcg acctcgagaa ggaacgcgag gcgctacgcg ccgagctcaa
     1981 ggagaccagc tcggaggcca agcgcaagaa gtacgtgaag cgactcaagc tggtcgaatc
     2041 cttcgtcgat tcggggagtc ggcccgagtg g
//
LOCUS       DATLKL010000532         1922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_153133, whole genome shotgun sequence.
ACCESSION   DATLKL010000532 DATLKL010000000
VERSION     DATLKL010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153133"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VM713_07145"
     CDS             <1..588
                     /locus_tag="VM713_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HVH84074.1"
                     /translation="RATAILVSGGLFMLRLVSITTLVLAGACTLARADVFRWVDTEGV
                     VHYSDQWVPGSVLVKTVRPHPETASAGAARTPSVTAMNGRATDQLNNEANARAVQQDV
                     AKARDAQCSFAKDRYAKAVTSRRVYKEGKDGERQYMTDADADAYREQARKDVQQACGN
                     VPEWKPDQAIPEPRPIPEPQPIPEPKVNPALATSR"
     gene            746..1663
                     /locus_tag="VM713_07150"
     CDS             746..1663
                     /locus_tag="VM713_07150"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVH84075.1"
                     /translation="MMRAGFVGLGAMGMHMARNLHRKGLLTAVWNRTQPKASALAAEL
                     HVAAPATLAELAAQVDAVVSCVSADADVLEVTRGLAPGFRSGALLLDCSTVSSETARQ
                     AAELLDGSGVEFLDCPVSGGVEGARDATLAIMVGGAPAAFERARPVLEALGKTISLFG
                     PVGSGQAAKATNQIMCAGIIEAVAEAMAFARAQGLPLPVLIDTLGKGAGSSWYFVHRA
                     PNMARGAYPAGFRVRLHEKDLRICHDMAARFGVELPVVERMLAEYAELVARGYGDEDI
                     SATFRLKEELFERTNIMGAPPLRAPPRTP"
     gene            1660..>1922
                     /locus_tag="VM713_07155"
     CDS             1660..>1922
                     /locus_tag="VM713_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84076.1"
                     /translation="MNEDSGDQHSLYLPDFCTSRTTLAIVLIVELTAIVLTLARQSIV
                     VDFWTDLVRTSLFLLWIGLSGAGLLCVLRGSLTRLSVAAGSTAV"
BASE COUNT          279 a          681 c          670 g          292 t
ORIGIN      
        1 cgggcaaccg ccatactggt gagcggaggc ctctttatgt tgcgactcgt atcgatcacc
       61 accctcgtgc ttgccggagc gtgcaccctg gcccgcgccg acgtgttccg ctgggtcgat
      121 acggaggggg tcgtgcatta cagcgatcag tgggtgccgg gctcggtcct ggtgaagacc
      181 gtaaggccac atccggagac ggcctctgcg ggtgccgcgc gcacgccatc ggttacggcg
      241 atgaacggac gggcgaccga tcagctcaac aacgaggcca acgcccgcgc cgtgcagcag
      301 gacgtcgcca aggcgcgcga cgcgcagtgc agcttcgcca aggatcgcta cgcgaaggcg
      361 gtcacctcgc gccgcgtgta caaggaaggc aaggacggcg agcggcagta catgacggat
      421 gccgacgccg atgcctaccg cgagcaagcc cgcaaggacg ttcagcaggc ctgcggcaac
      481 gtgccggagt ggaagcccga ccaggcgatt cccgaaccgc ggccgatccc ggagccgcag
      541 ccgatcccag agccaaaggt caaccccgcc ctggcgactt cgcggtaagc ggcgcgtccc
      601 aagcgccgcg cgccctccca agcggaccgt gttgtgagcg ccgcctgcgc ggcggcggtt
      661 ccgtgcgccc gcgctttcgc tctatagtgt cgctccgcgg ccgggccggc cgcacgaggt
      721 gcgccgccgc accgggtgtt cgcgagtgat gcgcgcaggt ttcgtcggac tcggcgccat
      781 gggcatgcac atggcgcgca acctccaccg caaggggttg ctcaccgcgg tctggaaccg
      841 cacccagccc aaggcgagcg cgctcgccgc cgagctccac gtggccgctc ccgccacgct
      901 cgccgagctc gccgcgcagg tcgatgcggt cgtctcgtgc gtctcggcgg atgccgatgt
      961 cctcgaggtg acgcgcgggt tggcacccgg attcaggtcg ggcgcgctgc tgctcgactg
     1021 ctccacggtg agctccgaga ccgcccgtca ggccgccgag ctcctcgacg gcagcggtgt
     1081 ggagtttctc gattgccccg tgagcggcgg cgtcgagggc gcgcgcgatg ccacgctcgc
     1141 catcatggtg gggggcgcgc cggccgcgtt cgagcgcgcc cggccggtgt tggaggcgct
     1201 ggggaaaacc atcagcctgt tcggaccggt cggctcgggc caggccgcca aggccaccaa
     1261 tcagatcatg tgtgccggca tcatcgaggc ggtcgccgag gcgatggcgt tcgcccgcgc
     1321 gcaggggctg ccgctcccgg tcctgatcga caccctcggc aagggggccg gctcgtcctg
     1381 gtacttcgtg catcgcgcgc ccaacatggc gcgtggcgcc tatcccgcgg gctttcgcgt
     1441 gcggctgcac gagaaggacc tgcgcatctg ccacgacatg gcggcgcgct tcggcgtaga
     1501 gctgccggtg gtcgagcgca tgctggccga gtacgccgag ctcgtggcgc gcggctacgg
     1561 cgacgaggac atctccgcca cgttccgcct caaggaggag ctgttcgagc gcaccaacat
     1621 catgggcgcg ccgccactgc gtgcgccgcc gcgcactcca tgaacgaaga ctccggcgac
     1681 cagcactcgc tctatctccc ggacttctgc acctcgcgca ccacgctggc catcgtgctg
     1741 atcgtggagc tcaccgccat cgtgctgaca ctggcgcggc agagcatcgt cgtcgatttc
     1801 tggaccgacc tggtgcgcac ctcgctgttc ctcctgtgga tcgggctcag cggagcgggg
     1861 ctgctgtgcg tgctgcgcgg ctccctcacg cgcctgagcg tagcggcggg ctccaccgcc
     1921 gt
//
LOCUS       DATLKL010000533         1730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_96629, whole genome shotgun sequence.
ACCESSION   DATLKL010000533 DATLKL010000000
VERSION     DATLKL010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96629"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /gene="gyrB"
                     /locus_tag="VM713_07160"
     CDS             <1..561
                     /gene="gyrB"
                     /locus_tag="VM713_07160"
                     /inference="COORDINATES: protein motif:HMM:NF036694.4"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HVH84077.1"
                     /translation="GFDRAEWLRSWTADLNERLNALADGTRTYRVEVRAAGAGQAARV
                     IVHKTEHGTTSHKYLPREFFESAEYQRIAELARTLAGLIGEGAYATRGGERAEIGSFK
                     EAMKWLFEQAKKGQTIQRYKGLGEMNPEQLWETTMDPAARTLLRVRAEDHTEADLMFT
                     TLMGDQVEPRREFIEKNALSVTNLDI"
     gene            726..1226
                     /locus_tag="VM713_07165"
     CDS             726..1226
                     /locus_tag="VM713_07165"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVH84078.1"
                     /translation="MNPLVPKLKQPIAALALGWCVAAGLPAPAAEPRNPRTAPPSEAR
                     QELLNLETEWTAAEEKHDEEALRRILDDRFIATFGAARTYDKEAFIVLFTAGDTDASK
                     SQTITDDAVIIDGDTAVLVGTDTARGTRDGVAYTVVYRYTTTYIRRHGHWVALAEHIV
                     QAPQAK"
     gene            complement(1364..>1730)
                     /locus_tag="VM713_07170"
     CDS             complement(1364..>1730)
                     /locus_tag="VM713_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVH84079.1"
                     /translation="IDRKAGHSAVAQVIEQGKDRLAEGDWVMIFPEGTRMPPGETRRY
                     GVSGTLLAAETGKFIVPVAHNAGHYWPRRGLRKKAGTVRVVIGTPIAAAGRDARAVNE
                     EIQAWVEATVKELMPAQDG"
BASE COUNT          328 a          568 c          557 g          277 t
ORIGIN      
        1 ggtttcgatc gcgccgagtg gctgcgcagc tggacagccg acctcaacga gcgcctcaac
       61 gcgctcgcgg acggcacccg aacctaccgc gtggaagtgc gcgccgcggg cgccggccag
      121 gccgcgcgcg tcatcgtgca caagaccgag catggcacca cgagccacaa atacctgccg
      181 cgcgagttct tcgagtcggc ggagtaccag cgcatcgccg agctcgcgcg gacgctcgcc
      241 ggcttgatcg gcgagggcgc ctacgcgacc cgcggcggtg agcgcgctga gatcggctcc
      301 ttcaaggagg cgatgaagtg gttgttcgag caggccaaga agggtcagac catccagcgc
      361 tataagggcc tcggcgagat gaatcccgag cagctctggg agacgacgat ggatccggcg
      421 gccaggacgc tgcttcgcgt ccgcgccgag gatcatacgg aagcggacct gatgttcacg
      481 accttgatgg gcgatcaggt cgagccacgg cgcgagttca tcgagaagaa cgcgctctcc
      541 gtcaccaacc tcgacatctg agggcccagg agccgcgtgt tccacgtgga acactcctgg
      601 actcgaggcg tcgtcggttt cgattcaaag agatcctcca cgcgcgcaaa tgcctctcta
      661 atgcctcaat agagcgggcg agggcaggac cgtctgaccg cccgaatcga acgggagtcc
      721 aagccatgaa ccctctggtg ccgaagctga agcaacccat tgcggccctg gcgctcggct
      781 ggtgcgtggc ggccgggctt ccggccccgg ctgcagagcc gaggaatccg cggaccgctc
      841 cgccgtccga ggcgcgacag gaacttctca acctcgagac ggagtggacg gccgctgaag
      901 agaagcacga cgaggaggcg ctccggcgca ttctcgatga ccgattcatt gcgactttcg
      961 gtgctgcgag aacctacgac aaggaagcct tcatcgtgct attcaccgcc ggcgataccg
     1021 atgcgtcgaa atctcagacc atcactgacg atgcagtgat catcgatggc gataccgcgg
     1081 tgctggtggg taccgacacc gcacgcggga cgagggacgg ggtcgcctac acggtggtgt
     1141 atcggtacac gacgacctac atccgccgcc acgggcactg ggtcgcgctg gcagagcaca
     1201 tcgtccaagc gccgcaggcg aagtagggtg cgaggtggga ggcgagtgcc ctgcctcagc
     1261 gcggctaagg cgagcccgat ccgagcatgg cgctattaga ccaataaaga tacaaagttg
     1321 cttggaaatg ccaagtatta acttctaatg agacaatctg cggctagcca tcctgcgcgg
     1381 gcatgagctc cttcaccgtc gcctcgaccc acgcctgaat ctcctcgttc accgcgcgcg
     1441 catcgcgtcc ggccgccgcg atcggcgtgc cgatgacgac ccgtaccgtg cctgccttct
     1501 tccgcagccc ccggcgtggc cagtagtgcc cggcgttatg cgcgaccggc acgatgaact
     1561 tgccggtctc ggccgcgagc agggtgcccg acacaccgta gcggcgcgtc tccccgggcg
     1621 gcatgcgtgt gccctcggga aaaatcatta cccagtctcc ctcggcgagt cggtccttac
     1681 cctgctcgat gacctgcgcg accgccgagt gtcccgcctt gcggtcgatg
//
LOCUS       DATLKL010000534         2486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_209267, whole genome shotgun sequence.
ACCESSION   DATLKL010000534 DATLKL010000000
VERSION     DATLKL010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209267"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1630)
                     /locus_tag="VM713_07175"
     CDS             complement(26..1630)
                     /locus_tag="VM713_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVH84080.1"
                     /translation="MPRIASRVDPHSQEYRDNERAMRALSEDLRREVGRAARGGSKAA
                     VDKHRAAGKLSARERVRVLLDTGSPFLELSQLAAHGMYGGDIACAGIITGIGRVGGRE
                     CVIVANDPTVKGGTYYPMTVKKHLRAQEIARENCLPCVYLVESGGAFLPAQDEVFPDR
                     DHFGRIFYNQATLSGLGIAQISVVHGSCTAGGAYVPAMSDENVIVRNQGRVFLGGPPL
                     VKAATGEDIDAETLGGADVHCRESGVCDHYAENDTHALAIARRIVARLRPAADSAPPR
                     AAREPRYDPAELYGVVPASLRKPYDAREVIARLIDDPELDEFKELYGTTLVCGFAHLG
                     GYPVGILANNGVLFSESALKGTHFIELCTREAVPLLFLQNITGFMVGRRAEAGGIAKD
                     GAKLVTAVACAPVPKLTVIVGGSYGAGNYAMCGRAYAPRLLYQWPNARISVMGGEQAA
                     SVLATVKRDQLARAGEQWPAAEEEAFKAPIRDQYERQGHPYYSSARLWDDGVIDPTET
                     RRVLSLSLAAVRHAPLEPARFGVYRM"
     gene            1700..>2486
                     /locus_tag="VM713_07180"
     CDS             1700..>2486
                     /locus_tag="VM713_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVH84081.1"
                     /translation="MNPLDCTDSDFGLGPELEEIRREVRRFATEQIAPRAADIDRSNE
                     FPRDLWPALGAQGLLGITVPERLGGAGLGYLAHVVVMEEISRASGAVGLSYGAHSNLC
                     VNQLRLNASAEQCARYLPKLISGEHVGALAMSEPGSGSDVVSMQLKATRRGDSYVLDG
                     RKMWITNGPDADVVMVYAKTDPAAGARGISAFIVERGTPGFSSAQKLDKLGMRGSSTC
                     ELLFDDCFVPAENLLGSENGGVRVLMSGLDYERVVLAGGSLGLM"
BASE COUNT          368 a          845 c          885 g          388 t
ORIGIN      
        1 gaccagtagc gtcttcacct gtggatcaca tccgataaac gccgaagcgc gccggctcga
       61 gcggcgcgtg gcgcactgcg gcgagcgaca gcgagagcac ccggcgcgtc tcggtggggt
      121 cgatcacccc atcgtcccac agccgcgccg aggagtagta agggtgcccc tgccgctcgt
      181 attggtcgcg gatcggcgcc ttgaacgcct cctcctcggc agcgggccac tgctcccccg
      241 cgcgcgcgag ctgatcgcgc tttacggtcg cgagcacgct cgcggcctgc tccccgccca
      301 tgacggagat gcgcgcgttc ggccactgat agaggaggcg cggtgcatag gcgcgcccgc
      361 acatggcgta gttgcccgcg ccgtagctgc cgccgacgat caccgtgagc ttcggcaccg
      421 gcgcgcaggc aaccgcagtg acgagcttgg cgccgtcttt ggcgataccg ccggcttccg
      481 cgcggcggcc caccatgaac ccggtgatgt tctgcaggaa gagcagcggg acagcttcgc
      541 gggtgcacag ctcgatgaag tgggtgccct tgagcgcgct ctcggagaac agcacgccgt
      601 tgttggcgag gatcccgacc ggatagccgc cgaggtgcgc gaagccgcac acgagcgtgg
      661 tgccgtagag ctccttgaac tcgtcgagct ccgggtcgtc gatgagccgc gcgatgacct
      721 cgcgcgcgtc gtagggcttg cgcaagctcg ccggaaccac gccgtagagc tcggccgggt
      781 cgtagcgcgg ctcgcgtgcg gcgcgcggcg gcgcgctgtc ggcggcgggc cgcaggcgcg
      841 cgacgatgcg gcgcgcgatg gccagcgcgt gcgtgtcgtt ctcggcgtag tgatcgcaca
      901 cgccggactc gcggcaatgg acgtcggcgc cgcccagggt ctcggcgtcg atgtcctcgc
      961 cggtcgcggc cttgaccagc ggcggaccgc cgaggaatac gcggccctgg ttgcgcacga
     1021 tgacgttctc gtccgacatc gcgggcacat aagcgccgcc ggcggtgcac gagccgtgca
     1081 ctacggagat ctgcgcgata ccgagaccgg agagcgtcgc ctggttgtag aagatgcggc
     1141 cgaagtggtc acgatcgggg aacacttcgt cctgcgccgg caggaacgcg ccgcccgact
     1201 cgacgagata cacgcacggc aggcagttct cgcgcgcgat ctcctgggcg cgcaggtgct
     1261 tcttcaccgt catggggtag taggtgccgc ccttgacggt ggggtcgttg gcgacgatca
     1321 cgcactcgcg gccccctacc cggccgatgc cggtgatgat tccagcgcag gcgatgtcgc
     1381 cgccgtacat gccgtgggcg gcgagttgtg agagctcgag aaaggggctg ccggtgtcaa
     1441 gcagcacccg cacgcgctcg cgggcagaga gcttgcccgc agcgcggtgc ttgtccacgg
     1501 cggccttgga gccgccgcgg gcggcacgcc ccacctcgcg gcgcaggtcc tcgctgaggg
     1561 cgcgcatggc gcgctcgttg tcacggtatt cctgggaatg cgggtcgacg cggctggcga
     1621 tgcggggcat gcgtacttgc catgattgcc tgtttgtccg acaatcatac gatctggtcc
     1681 aaggagaagg caagccgtca tgaacccgct cgactgcacc gacagcgact ttggcctggg
     1741 ccccgagctc gaggagatcc gccgcgaggt gcggcgcttc gctaccgagc agatcgcccc
     1801 gcgcgccgcc gacatcgacc gcagtaacga atttccgcgc gacctgtggc cggccctggg
     1861 tgcccagggc ctgctgggca tcaccgtgcc cgagcgcctc ggcggcgccg gcctcggcta
     1921 tctcgcccac gtcgtcgtca tggaggagat ctcgcgcgcc tcgggggcgg tcgggctttc
     1981 ctatggcgcg cactccaacc tgtgtgtcaa ccagctgcgg ctgaatgcca gcgccgagca
     2041 gtgcgcgcgc tatctgccga agctcatctc cggggagcac gtcggcgcgc tcgccatgtc
     2101 ggagcccggc tccggctccg acgtggtgtc gatgcagctc aaggccacgc gccgcggcga
     2161 ctcctacgtg ctggatggcc gcaagatgtg gatcaccaac ggcccggacg ccgacgtcgt
     2221 catggtgtac gccaagactg acccggcggc cggcgcgcgc ggcatcagcg ccttcatcgt
     2281 cgagcgcggc accccgggct tcagcagcgc gcagaagctc gacaagctcg gcatgcgcgg
     2341 ctcgagtacc tgcgagctgc tgttcgatga ctgcttcgtg cccgccgaga acctgctcgg
     2401 gagcgagaac ggcggcgtgc gcgtgctcat gagcggactc gattacgagc gcgtggtgct
     2461 cgccgggggc tcgctcggac tgatgg
//
LOCUS       DATLKL010000535         1569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_51416, whole genome shotgun sequence.
ACCESSION   DATLKL010000535 DATLKL010000000
VERSION     DATLKL010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51416"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /gene="hisS"
                     /locus_tag="VM713_07185"
     CDS             complement(<1..1182)
                     /gene="hisS"
                     /locus_tag="VM713_07185"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HVH84082.1"
                     /translation="MTEEIQPIRGVNDVLPAEIGAWQHLEAVTRELLAAYGYEEIRVP
                     VLERTELFRRAIGEHTDVVGKEMYTFTDRGGDSLTLRPEATAGIVRALISNGMLRGQR
                     HKLWCLGPMFRHEAPQAGRYRQFWQVDVEAVGCPGPDVDAELIALSARLWQRLGLTGL
                     RLEINSLGTPESRRAYRARLIEYFNAHEARHDADSRRRLGGNPLRILDSKNPEMQELI
                     AGAPLLTEHLDAESREHFEALRRMLDGAGVPYDINPRLVRGLDYYNRTVFEWITAATG
                     AQNAVCSGGRYDGLIAQLGGDATPAVGFAMGVERLVTQLVAAGGTPPAAHPDVYVVVS
                     GARATTEALTIVERLRSERPGLRFELNLGGGNFKAQFRRADKSGAPLALIIGDDELAR
                     GV"
     gene            complement(1179..>1569)
                     /locus_tag="VM713_07190"
     CDS             complement(1179..>1569)
                     /locus_tag="VM713_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HVH84083.1"
                     /translation="ELPAGVRGSASAQRLVQAVAAAHSASAAATSTATAAQLALRFSE
                     VSWVAVYDGSGKRLEEGFNRPDSTRMVTGAPPLRVVLGNAPGVTVSLNGQKVALDGLV
                     RRDGTARFVLDGNGHAAEVPPLVVAHQ"
BASE COUNT          221 a          560 c          555 g          233 t
ORIGIN      
        1 taccccgcgc gcgagctcat catcgccgat gatcagcgcg agcggcgcac cgctcttgtc
       61 ggcgcggcgg aactgcgcct tgaagttgcc cccaccgaga ttcagctcga atcgcagtcc
      121 ggggcgctcg ctgcgcagcc gctcgacgat cgtgagcgcc tccgtggtgg cgcgcgcgcc
      181 gctcacgacg acgtagacat cggggtgcgc cgcgggcggt gtgccgccgg cggcaacgag
      241 ctgcgtcacc agccgctcga cgcccatggc aaagccgacc gcaggggtcg cgtccccgcc
      301 gagctgcgcg atcagcccgt cgtagcggcc gccggagcat acggcattct gggcgccggt
      361 ggcggccgtg atccactcga acacggtgcg gttgtagtag tcgagcccgc gcaccaggcg
      421 cgggttgatg tcataaggca cccctgcccc atccagcatg cggcggagcg cctcgaagtg
      481 ctcgcgggat tcggcatcga gatgctcggt gaggagcggc gcgccggcga tgagctcctg
      541 catctcggga ttcttgctgt cgaggatgcg cagcggattg ccgccgaggc gccgccggct
      601 gtcggcgtcg tgccgagcct cgtgggcgtt gaagtactcg atgagcctgg cgcggtaagc
      661 gcggcgggac tcgggagtgc cgagggagtt gatctcgagc ctgaggcccg tgagcccgag
      721 gcgctgccag agccgcgcgc tgagcgcgat gagctccgcg tcgacgtcgg gcccgggaca
      781 accgaccgcc tcgacgtcca cctgccagaa ctgccggtag cgccctgcct ggggcgcctc
      841 atggcggaac atcgggccga ggcaccagag cttgtggcgc tgaccgcgca acatgccgtt
      901 cgagatgagc gcgcgcacga tgccggcggt cgcctccggg cgcagcgtga gactgtcgcc
      961 gccgcggtcg gtgaaggtat acatctcctt gccgaccacg tcggtatgct cgccgatcgc
     1021 gcgcctgaag agctcggtgc gctcgagcac cggcacgcgg atctcctcgt agccatacgc
     1081 ggcgagcagc tcccgcgtca ccgcctcgag atgctgccag gcgccgatct cggcgggcag
     1141 cacgtcgttc acgccgcgga tcggctggat ctcctccgtc actggtgcgc cactaccagc
     1201 ggcggcacct ctgcggcgtg cccgttgccg tcgagcacga agcgcgccgt accgtcgcgg
     1261 cgcactagcc cgtcgagcgc caccttctgg ccgttgagac ttactgtcac acccggcgca
     1321 ttgccgagca ccaccctgag cggcggcgcg ccggtgacca tgcgcgtgct gtcgggcctg
     1381 ttgaatccct cctccaggcg ctttcccgag ccgtcgtaca ccgcgaccca gctcacctcg
     1441 gagaacctga gggcaagttg cgccgcggtg gcggtggaag tcgcggcggc gctcgcggag
     1501 tgcgcggcgg cgaccgcctg cacgagccgc tgcgcggagg ccgagccccg cacgcccgcc
     1561 ggcagctct
//
LOCUS       DATLKL010000536         2820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_175418, whole genome shotgun sequence.
ACCESSION   DATLKL010000536 DATLKL010000000
VERSION     DATLKL010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175418"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VM713_07195"
     CDS             <1..886
                     /locus_tag="VM713_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVH84084.1"
                     /translation="VSDEILKRMADSYRRMRNTLRFLLGNLDGFSPARDAVSVDELVA
                     LDAYALARTRALQAEVLEAYRRYAFHLIYQKVHNFCSVDLGGFYLDVLKDRLYTTPAH
                     GRARRSAQTAMSHIAECMVRWLAPILSFTAEEVWRYLPGERAESVFHVTWHTPPEVPT
                     RAIDWEALIELRTDVTRELEKLRDTGAIGAPLDATIDVYCVPGEYPRFAALGPELRFL
                     LITSEARVHEVAAGPSGAVPAANLPGVWIAAAANTDPKCVRCWNRRPDVGTHTHHPQL
                     CGRCIVNLEMPGEERRYV"
     gene            883..1398
                     /gene="lspA"
                     /locus_tag="VM713_07200"
     CDS             883..1398
                     /gene="lspA"
                     /locus_tag="VM713_07200"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019486629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HVH84085.1"
                     /translation="MSISSIPAAASGWRWLPLTATVIVADQASKLWIVHHFSFLQRVR
                     LLPVLDVILTYNTGAAFSMLADAAGWQRWVFVALALIVSVALVVWLRRLAAATHALIA
                     CGLALIVGGALGNMLDRLRLGHVIDFIHAHWGAHYFPAFNVADSAITVGAGLLLLDAW
                     REARTARRGSG"
     gene            1398..2369
                     /gene="ispH"
                     /locus_tag="VM713_07205"
                     /gene_synonym="lytB"
     CDS             1398..2369
                     /gene="ispH"
                     /locus_tag="VM713_07205"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017387043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HVH84086.1"
                     /translation="MQVLLANPRGFCAGVDRAIDIVERALGLFGAPIYVRHEVVHNRH
                     VVERLRTLGAVFVDELAEVPSGCTVIFSAHGVSSAVEQEAHGRRLTVFNATCPLVTKV
                     HMEVQRYAREGRDVILIGHAGHPEVEGTMGRFDESSGGRMHLVQDLEDVAALDVRAGR
                     ELALVTQTTLSVDDTAEIVAALRARFPALATPRHEDICYATQNRQDAVKKLLEQCDVL
                     VVVGSRTSSNSNRLRELAERAGVPGYLVDGPDELRAEWFAGKRAVGVTAGASAPELQV
                     QQVIARLAQWGAEPPREVTGREERVFFALPRELRRAETPEGTEPAKR"
BASE COUNT          386 a         1000 c          970 g          464 t
ORIGIN      
        1 cgtctcggac gagatcctca agcgcatggc ggattcctac cgccgcatgc gcaacacgct
       61 gcggttcctg ctcggtaatc tcgacggctt ctccccggcg cgcgatgccg tgtccgtgga
      121 tgagctcgtg gcactcgacg cttacgcgct cgcgcgcacg cgcgcgctgc aggcggaagt
      181 cctcgaggcg taccgccgct atgcctttca tctgatctat cagaaggtgc acaatttctg
      241 cagcgtggat ctcggcggct tctatctcga cgtgctcaag gaccgcctgt acaccacgcc
      301 cgcccacggc cgcgcgcgcc gctcggctca gaccgcgatg agtcacatcg ccgagtgcat
      361 ggtgcgctgg ctcgccccga tcctgtcgtt cacggccgag gaagtctggc gctatcttcc
      421 gggggagcgg gccgagtcgg tgtttcacgt cacctggcac acgccgcccg aggtcccgac
      481 gcgcgccatc gactgggagg cgctcatcga gctgcgcacc gatgtgacgc gggagctcga
      541 gaagctgcgc gatacgggcg ccatcggcgc gcccctcgat gccacgatcg acgtgtactg
      601 tgtgcccggg gagtacccgc gcttcgcggc gctcggtcct gagctgcgct tcctcctcat
      661 cacgtccgag gcgcgggtgc acgaggtcgc ggcaggcccg tccggcgcgg tcccggcggc
      721 gaatctgccg ggcgtatgga tcgccgccgc ggccaacacc gacccgaagt gcgtgcgctg
      781 ctggaaccgg cggccggacg tcgggacgca cactcaccat ccgcaactgt gcgggcgctg
      841 tatcgtcaac ctcgagatgc cgggcgagga gcggcgttac gtatgagcat ctcctcgatc
      901 ccggccgccg ccagcggctg gcgctggctg ccgctgaccg ctacagtgat cgtggccgac
      961 caggcgtcca agctctggat cgtgcaccac ttctccttcc tgcagcgggt gcgtctcctg
     1021 ccggtgctcg acgtcattct gacttacaac acaggcgccg ccttcagcat gctggcggac
     1081 gccgccggct ggcagcgctg ggtattcgtc gccctagcgc ttatcgtgag cgtggcgctg
     1141 gtcgtgtggc tcaggcgcct cgcggccgcg acccacgcac tgatcgcctg cgggctcgcg
     1201 ctcatcgtcg gcggggcgct cggcaacatg ctcgaccggc tgcggctcgg ccacgtgatc
     1261 gacttcatcc atgcccactg gggagcgcac tactttccgg ccttcaacgt cgccgactcg
     1321 gcgatcaccg tgggcgcagg gttgctgctg ctcgatgcct ggcgcgaagc gcgcaccgct
     1381 cgccggggga gcggctgatg caagtgctgc tcgccaaccc ccgcggcttc tgcgccggtg
     1441 tcgaccgtgc gatcgacatc gtcgagcggg cgctcgggct cttcggtgcg ccgatctacg
     1501 tgcggcacga ggtggtccac aaccgccacg tggtcgagcg gctgcgcacc ctcggcgccg
     1561 tcttcgtcga cgagctcgcc gaggtgccct ccggctgcac cgtgatcttc tcggcccacg
     1621 gggtctcgag cgccgtcgag caggaggcgc acggccgccg cctcacggtg ttcaacgcca
     1681 cctgcccgct ggtcaccaag gtgcacatgg aggtgcagcg atatgcgcgc gaagggcgcg
     1741 atgtgatcct catcggccac gccggccacc cggaggtgga aggcaccatg ggccgattcg
     1801 acgaatcctc aggcggacgt atgcacctgg tgcaggacct cgaggatgtc gccgccctcg
     1861 acgtgcgcgc ggggcgcgag ctcgcgctcg tcactcagac gacgctctcg gtggacgaca
     1921 ccgccgagat cgtcgccgcg ctgcgggcgc gcttccccgc gctcgccacg ccgcgtcacg
     1981 aggatatctg ttacgccacg cagaaccgtc aggacgccgt caagaaactg ctggagcaat
     2041 gcgatgtgct cgtggtcgtg ggctcgcgca ccagctccaa ctccaaccgc ctgcgtgagc
     2101 tcgccgagcg ggccggggtg cccggctatc tggtggacgg gcccgatgaa ttgcgcgccg
     2161 agtggttcgc gggcaagcgc gccgtcggcg tcaccgccgg cgcctcggct ccggagctgc
     2221 aggtgcaaca ggtgatcgcg cgcctggcgc agtggggcgc cgagcccccc cgcgaggtga
     2281 ccggacgcga ggagcgcgtc ttcttcgcgc tgccgcgcga gctgcggcgc gcggaaacac
     2341 ccgagggcac cgagcccgcc aagcgctagc tggcgctcgc ccggggggct gtcgctgcgt
     2401 tgcgtcaccc ggggcgatct gcgttccctc gcgtcctggg cgaaaccggc gccgctcccg
     2461 cagacactta ccggcagggt aaccagtcag cggggacttc ctatggacgc gcgtcatccg
     2521 atcctgagcc gattccccat gattattgcc ggcgcactcc tcgcgacggc cttcaccgcc
     2581 tgcggcggcg gctacggtgg tggtggaggt ggtggtggtg gaggaggcat gtgtggcggg
     2641 gcctaccagc cgatgtgccc gccgccgatg gtctcgatta ctgccccgat ggccggggtc
     2701 gtggtcaccg tgtcggacgg ccacgcggcg gcggccatgt cgccagcgca gatcttcccc
     2761 acgccggcat cgaaggctgc ggggatggcg cgcatccgcg tcgcccagga caccggtgcc
//
LOCUS       DATLKL010000537         1718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_198205, whole genome shotgun sequence.
ACCESSION   DATLKL010000537 DATLKL010000000
VERSION     DATLKL010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198205"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="VM713_07210"
     CDS             complement(<1..1399)
                     /locus_tag="VM713_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HVH84087.1"
                     /translation="MHRTLSVAITGLALAQATLPLPCRAQPAHGAATVSAGDARFEFL
                     TPSLLRMEYARSADFADAPSAVVLKRDWPAVGVETRERDGWLIAATSAVMLRYRLHSG
                     AFTAANLEVRWKDRSGGERTWHPGDVDSANLGGLTYSLDNISARNLPKDGMDLESPVD
                     DSIPGIDLVLPQAQPGLLSRSGYAFIDDSETPLWNAQRRWVEPRRTRGQDWYLFTYDR
                     DYQRVLQEYAELCGPIPMIPRHVLGPMITDFNFEYFPGSAESRRPDFARYNQQYLMGE
                     VARMRASHIPFDTLVLDFAWHDYGWDGGYDWSPLFPRPLELMRSLHGRGVKLSLNDHP
                     GYIHTDESILSFDDSHAGEVLAALGRVAPAKPSFNLDLSQGWSFSTDPRTGRWQPIRI
                     GLPWEEQQHAGYRGVGWYRKSLRLPAQLPQKLYLYVGEVRKAYRAIVNGKEVAPSQIH
                     WPQRLTYSDITAAVVP"
     gene            complement(1458..1592)
                     /locus_tag="VM713_07215"
     CDS             complement(1458..1592)
                     /locus_tag="VM713_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84088.1"
                     /translation="MLPLFAPRHDDYSAACSVNTPLVGGTRLTADVLVADSSPATASR
                     "
BASE COUNT          291 a          561 c          583 g          283 t
ORIGIN      
        1 cgggcactac ggccgcggtg atgtccgaat aggtgagacg ctgcggccag tgaatctggc
       61 tcggcgcgac ctccttccca ttgacgatcg cacggtaggc cttgcgaacc tcgccgacat
      121 agagatagag cttctgcggg agctgcgccg gcaggcgcag cgacttgcgg taccaaccga
      181 caccgcggta gcccgcgtgc tgctgctcct cccacggcag gccgatgcga atcggctgcc
      241 agcgccccgt tcggggatcg gtggagaaac tccagccctg ggagaggtcg agattgaagg
      301 atggcttcgc gggcgccacc ctgccgagcg cggccagcac ctcgccagcg tgactgtcgt
      361 cgaacgacag gatgctctcg tcggtgtgaa tgtagcccgg gtgatcgttg aggctcagct
      421 tcactccacg cccgtgcagc gaacgcatga gctcaagcgg ccgtggaaag agcgggctcc
      481 agtcgtaacc tccgtcccag ccgtagtcgt gccaggcgaa atcgagcacc agtgtgtcga
      541 agggaatgtg gctggcgcgc atgcgcgcca cctcgcccat gaggtattgc tggttgtagc
      601 gggcgaaatc cggccgccgc gactcggcgc tgccgggaaa gtactcgaag ttgaaatcgg
      661 tgatcatcgg gcccagcaca tggcgcggaa tcatcggaat cggcccgcag agctcggcgt
      721 actcctgcag cacgcgctga tagtcgcggt cgtaagtgaa cagataccag tcctggccgc
      781 gcgtgcgccg tggctcgacc cagcggcgct gcgcattcca cagtggcgtc tcgctgtcgt
      841 cgatgaaggc atagccgctg cggctcagga gccccggctg tgcctgcggc agcacgaggt
      901 cgatccctgg aatcgaatca tcgaccgggc tctcgagatc catgccgtcc ttcggcagat
      961 tccgcgcgct gatgttgtcg agcgagtagg tgagcccgcc gagatttgcc gagtcgacgt
     1021 cccctggatg ccaggtacgc tcacccccgg agcggtcctt ccagcgcacc tcgagatttg
     1081 cggccgtgaa ggccccggag tgcaggcggt aacggagcat gacagcgctg gtcgcggcga
     1141 tgagccaccc gtctcgctcg cgggtttcga cgccgaccgc cggccagtct cgcttcagca
     1201 cgaccgcgct cggcgcatcg gcgaagtcag cggagcgcgc gtattccatc cgcagcagcg
     1261 acggtgtcag gaactcgaag cgggcgtccc ccgcagagac cgtggctgcg ccgtgcgctg
     1321 gctgcgcccg gcagggcagc ggcagcgtcg cctgtgcgag agcgaggccg gtaatcgcga
     1381 cgctgagagt ccgatgcatg cgcagtgcct cacgtcccgc ctctgcggac ggacgttacg
     1441 cctacgctaa ctcagcgtca gcgggatgcg gtagccgggc tcgagtctgc gaccagcacg
     1501 tccgccgtca gccgcgtgcc gccgaccagc ggagtgttca ccgagcaggc ggcgctgtag
     1561 tcgtcgtggc gaggcgcgaa cagcggcagc acgtcccgct tgagttgcga gatcagctca
     1621 cagtgcgacc tcgatctgct cgcgtacggg cacgagcgca gcgtcagtcc gcagctgacg
     1681 cttccgcatc ctgggatagt ggagcgcaac ttcgttct
//
LOCUS       DATLKL010000538         1763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_198217, whole genome shotgun sequence.
ACCESSION   DATLKL010000538 DATLKL010000000
VERSION     DATLKL010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198217"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..>1763)
                     /gene="dnaE"
                     /locus_tag="VM713_07220"
     CDS             complement(58..>1763)
                     /gene="dnaE"
                     /locus_tag="VM713_07220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HVH84089.1"
                     /translation="LESRGMKDLIRRLQPDCFEDIVAPVALFRPGPLQSGMVDDFINR
                     KHGRSDGPIDYLHPSLAPVLKPTYGVILYQEQVMQIAQTLAGYTLGGADLLRRAMGKK
                     KPEEMAQQRSVFTAGAVARGVRERLAEHIFDLMEKFAGYGFNKSHSAAYALLAYQTAY
                     LKAHHPAAFMAAVLSADMDHTDKVVTLIKECSDMGLEVLPPDVNASGYQFAADGARRI
                     RYGLGAVRGVGAGAVEGLIEERGARGAYSSLEDLCRRLDLTKVNRRVLEALLRSGSLD
                     ALGANRATLMDRLASAMQLGDQNTRAHAAGQDDLFGGLSAVEPPPQPQRAAVLPEWSE
                     AVRLSGERETLGLYLTGHPLRRFEASLARFVSHRIGDLVGDRPLTGLEPGRFGAGRPV
                     TVAGLVDEVKKRGPRVILTLDDRTGRLEVMLFEETWQKHRDLIVKDALVLVEGALRFD
                     EFSDSWRLAARRVSELERLREQEARRLVLRCSHADLPRLSERLAAILEPWRPGPCPVT
                     IEYATASASGALNLGAEWSVRASRELLEQLEGLVGSGALEVLYAAPAASAGGAWSADG
                     R"
BASE COUNT          260 a          618 c          631 g          254 t
ORIGIN      
        1 gcatgcggcg gagcggcgag gttacgtaag gagcggactt gagcgcaagt tgagccgtca
       61 gcggccgtcg gccgaccagg caccgcccgc gctcgccgcg ggcgctgcgt acagcacctc
      121 gagggcgccg ctccctacca ggccctcgag ctgctcgagg agctcgcggc tcgcgcgcac
      181 gctccactcc gcgcccagat tgagcgcccc gctcgccgag gcggtcgcgt actcgatggt
      241 gaccggacag gggccggggc gccacggctc gaggatcgcg gcgaggcgtt cgctgaggcg
      301 cggcagatcg gcgtgcgagc aacgcagcac gaggcgacgc gcctcctgct cgcgcagccg
      361 ctcgagctcg ctcaccctac gcgccgcgag ccgccacgag tcgctgaact catcgaagcg
      421 cagcgccccc tcgaccaaca ccagggcgtc cttgactatg aggtcgcggt gcttctgcca
      481 ggtctcctcg aatagcatga cctcgaggcg cccggtgcga tcgtcgagcg tcaggatcac
      541 gcgcgggccg cgcttcttca cctcgtcgac gaggcccgcg accgtgaccg ggcggccggc
      601 gccgaagcgc cccggctcga gcccggtgag cggccggtcg ccgaccaggt caccgatgcg
      661 gtgcgagacg aagcgcgcga ggctcgcctc gaagcgccgc agcggatggc cagtgaggta
      721 gaggccgagt gtctcgcgct cgccgctgag gcgcactgcc tcgctccatt cgggcagaac
      781 cgcggcgcgc tgcggctgcg gcggcggctc cacggcgctc aagccgccga acagatcgtc
      841 ctgcccggcc gcgtgggcgc gcgtgttctg gtcgccgagc tgcatggcgc ttgcgagccg
      901 atccatgagc gtggcgcggt tggcaccgag cgcatcgagg ctcccggagc gcagcagcgc
      961 ctcgagcacg cgccggttga ccttggtgag atcgagacgc cggcacagat cctcgaggct
     1021 cgagtaggct ccacgcgcgc cgcgctcctc gatcaggccc tcgaccgcac ccgcgccgac
     1081 accgcgcacg gcgccgaggc cgtagcggat gcgccgcgcg ccgtcggctg cgaactgata
     1141 gcccgaagcg ttgacatccg gcggcagcac ctcgagcccc atgtcgctgc attccttgat
     1201 gagcgtcacc accttgtcgg tgtggtccat gtcggccgac agcacggcgg ccatgaacgc
     1261 cgccggatgg tgcgccttca ggtaggcggt ctggtacgcg agcagcgcat aggcggcgga
     1321 gtgcgacttg ttgaaaccgt agccggcgaa cttctccatc agatcgaaga tgtgctcggc
     1381 gaggcgctcg cgcacgccgc gtgccaccgc cccggcggtg aacaccgagc gttgctgcgc
     1441 catctcctcg ggcttcttct tgcccatcgc gcgccgcagc agatcggcgc cgccgagcgt
     1501 gtagccggcg agcgtctgcg cgatctgcat cacctgctcc tggtagagga tgacgccgta
     1561 ggtgggcttg aggacgggtg cgaggctcgg atggaggtag tcgatcgggc cgtcgctgcg
     1621 gccgtgcttg cggttgatga agtcatcgac catgccggac tgcagcggcc ccgggcgaaa
     1681 cagcgcgacg ggcgcgacga tgtcctcgaa gcagtcgggc tggagccggc ggatgaggtc
     1741 cttcatgccg cgcgattcga gct
//
LOCUS       DATLKL010000539         3187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_85395, whole genome shotgun sequence.
ACCESSION   DATLKL010000539 DATLKL010000000
VERSION     DATLKL010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85395"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VM713_07225"
     CDS             complement(<1..571)
                     /locus_tag="VM713_07225"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HVH84090.1"
                     /translation="MSPGRSAALGLAVAGILLGSGTAGFLLSRHLGPGRPVLRPAAPA
                     SVGAPTAEARPAARPIPERLPDFSLPDLAGAPHRLADWRGRPLAVNFWATWCEPCRRE
                     IPLLKALRRENSINRLEVVGIALDHPDSVLKFVRELGIEYPILVGEKGGLEAVAAFGV
                     DTVLPFTVFADAEGRIVTLKIGELHRDEAT"
     gene            complement(568..2304)
                     /gene="dsbD"
                     /locus_tag="VM713_07230"
     CDS             complement(568..2304)
                     /gene="dsbD"
                     /locus_tag="VM713_07230"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HVH84091.1"
                     /translation="MSSVLGSVKTTGDDFLPVDQAFRFDALAGGSDQVLLNWEIAEGY
                     YLYRARIKIVTPSARAQLGAPQFPPGEFKNDEYFGRQEIYHHELRVSVPVARAAGAAF
                     ALPLEVTYQGCAEKGLCYPPTTKSVSVPLPAGSGNASGGAGPGDVAAGTSEQSRFAQL
                     LTGNFLLMVGGFYLAGLLLAFTPCVLPMVQILSGIIAGGGAQVTTGRAFALSLTYVLG
                     MALTYTAAGIAGAAMGREVQSLFQQWWVLALFAAVFVAMALSMFGLYTVQMPAAIQTR
                     IANVSNRQSAGTFGGVAIMGALSALIVTTCVGPALVAALLVISQTGQIGRGGVALFAM
                     SIGMGTPLLIVGASAGKLLPKAGAWMDFVKKLFGVMMLAVAAWMLARIVSDRIALLLW
                     AVPALALTWLLWFELKTGSGVRWALRVAGLAVGLYGVALAAGSALGGRDPLAPIPALA
                     HPSRELPFRSIRTTADLEREVHAASAAGRGVLLDFSADWCSSCKEMERYTFTDPGVQA
                     VLAHVSLLRADVTAGNADDQALLQHFGIIGPPTIAFYGPDGHERSQYRVVGYMKADAF
                     AAQARAALQSPP"
     gene            2661..2954
                     /gene="groES"
                     /locus_tag="VM713_07235"
     CDS             2661..2954
                     /gene="groES"
                     /locus_tag="VM713_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009684394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HVH84092.1"
                     /translation="MKIRPLHDRVIVKRLEEERTSPGGIVIPDTAAEKPVQGKIVAVG
                     KGKILEDGKVRPLDVKVGDKILFGKYSGTEVKVDGDELVVMREEDVMAAIEGK"
     gene            2969..>3187
                     /locus_tag="VM713_07240"
     CDS             2969..>3187
                     /locus_tag="VM713_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HVH84093.1"
                     /translation="MAAKEVRFSDDARARMFKGVNVLANAVKATLGPKGRNAVLEKSF
                     GAPTVTKDGVSVAKEIELKDKFENMGAQM"
BASE COUNT          637 a         1043 c         1066 g          441 t
ORIGIN      
        1 acgtcgcctc gtcccgatgc aactccccaa tcttgagcgt gactattcga ccctccgcat
       61 ccgcaaacac ggtaaagggc agcaccgtat ccactccgaa ggcggcgacg gcctcgaggc
      121 cgcccttctc gccgaccaga atgggatact caatgccgag ttccctgacg aatttcagca
      181 ctgaatcagg atgatcgagt gcgataccga ccacttcgag gcgatttatc gaattttcgc
      241 gacgcagtgc cttcagaaga ggtatctcgc ggcggcaggg ctcgcaccag gtcgcccaga
      301 agttgacggc gagcggccgg ccgcgccagt cggcgagccg atgcggggcg cctgccaggt
      361 ccggaaggga gaaatcgggc agccgctccg ggatgggtcg ggccgcgggg cgagcctctg
      421 ctgtcggcgc ccccaccgaa gcgggcgcgg ccggccgcag gacgggtctg cccggaccga
      481 gatggcggga gagcaggaaa cccgccgtac cagagccgag gagaattccc gcaaccgcaa
      541 gacccaaggc ggccgagcgc cccgggctca cggcggcgat tgcaacgcgg cgcgagcctg
      601 ggcggcgaag gcatcggcct tcatatatcc gaccacgcgg tactgactcc gctcatgccc
      661 gtcgggccca tagaaagcaa tagtcggggg tccgatgatt ccgaagtgct ggagcagcgc
      721 ctgatcgtcg gcgttgcccg cggtcacgtc ggcgcgcaac agcgagacgt gcgcaagtac
      781 agcctgtacc ccggggtcgg tgaacgtgta acgctccatc tccttgcagg agctgcacca
      841 gtcggcggag aaatcgagca ggactccgcg ccccgcggcg ctcgccgcgt gcacctcgcg
      901 ctcgagatcc gccgtggtgc ggatactgcg aaacggcagc tcccgcgacg gatgcgcgag
      961 cgcgggaatc ggtgccagcg gatcccggcc gccgagcgca gagccggcgg cgagcgccac
     1021 accgtagagg ccgaccgcga gcccggcgac gcgcagcgcc cacctgactc ccgaacccgt
     1081 cttgagctcg aaccacagca gccacgtgag cgccagcgcc ggcaccgccc agaggagcag
     1141 tgcgatgcga tcggagacga tgcgcgcaag catccacgcg gcgaccgcca gcatcatgac
     1201 gccgaatagc ttcttgacga agtccatcca cgcgccggcc ttcggcagga gcttgccggc
     1261 ggaggcgccg acgatcagga gcggcgtccc catgccgatg ctcatggcga acagcgccac
     1321 gcccccccgg ccgatctggc ccgtctggct gatgacgagc agcgctgcga cgagcgcggg
     1381 acctacgcag gtcgtgacga tgagggcgga caacgccccc atgatcgcca cgccgccgaa
     1441 agtaccggcg gactggcggt tgctgacgtt ggcgatgcgc gtctggatcg cggccggcat
     1501 ctgtacggtg tagaggccga acatcgacag cgccatggcg acgaaaaccg cggcgaacag
     1561 cgccagcacc caccactgct gaaacagcga ctgcacctcg cgacccatcg ctgccccggc
     1621 gatgccggcg gcggtgtagg taagcgccat gccgagcacg taagtcaacg agagcgcgaa
     1681 cgcgcgcccg gtagtcacct gcgcgccccc gccagcgatg atccccgaga ggatctgcac
     1741 catcggcagc acgcagggcg tgaaggcgag gaggaggccc gcgagataga aaccgccgac
     1801 catgagaagg aagttgccgg tgagcagctg cgcgaatcgc gactgctccg aggtgccggc
     1861 ggcgacatcg cccgggcccg cgccgccgct cgcgttgccg ctacccgcgg ggaggggaac
     1921 gctcaccgac ttcgtcgtgg gcggatagca caggcccttc tccgcgcatc cctggtaggt
     1981 cacctcgagc ggcagggcga aggccgcgcc cgcggcgcgc gccaccggca cgctcacgcg
     2041 cagctcgtgg tgatagatct cctggcggcc gaagtactca tcgtttttga attcgccggg
     2101 gggaaactgc ggcgcgccga gctgcgcccg cgcgctcggg gtcacgatct tgatgcgcgc
     2161 ccgatagagg taatagccct cggcgatctc ccagttgagc agcacctgat cgcttccgcc
     2221 ggccagcgcg tcgaaccgaa aggcctggtc cacggggagg aagtcgtctc cggtggtctt
     2281 gaccgatccg aggacactgc tcacgccagt ggcggggggc gcggccgcag ggccggccgg
     2341 cgcagacgcg ggctgggcgt gcacgccggc ggctatggcg cctgccagga gcagcccggc
     2401 agcaagaata tgtctaatgc gtgaggccat atgccggttt tatagcgcaa gccttggacg
     2461 agtggcagga actgaggttc atcccggaga agaccgcctg atgatcgcaa aaaaggcccc
     2521 accgaccaaa aaagatccgc ctacggcttg aaatcacagg gctcctccct atcattagca
     2581 gccgcgtggg gggagtgcta acaagccccg ctgcgaataa cccatccaat catcaagagt
     2641 tttttaagga gcgaacaccc atgaagattc gtcctcttca tgaccgcgtc atcgtgaagc
     2701 gcctggaaga ggagcgtacc tccccgggcg gcatcgtgat tcccgatacc gcggccgaga
     2761 agccggtgca gggaaaaatc gttgcggtcg gcaagggcaa gattctcgag gacggcaagg
     2821 taaggccgct cgatgtgaag gtcggcgaca agatcctgtt cggcaagtac tccgggaccg
     2881 aggtcaaggt cgacggtgat gagctcgtgg tcatgcgcga agaagacgtc atggccgcca
     2941 ttgaaggcaa gtaagcgagg atcacgaaat ggctgccaaa gaagttcgat tcagcgatga
     3001 cgcgcgcgcc cgcatgttca agggcgtcaa cgttctcgcc aacgccgtca aggcaaccct
     3061 cggtcccaag ggccgcaacg ccgtgctcga gaagagcttc ggcgcaccca ccgtcaccaa
     3121 ggacggcgtc tcggtcgcga aggagatcga gctcaaggac aagttcgaga acatgggcgc
     3181 gcagatg
//
LOCUS       DATLKL010000540         5840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_74297, whole genome shotgun sequence.
ACCESSION   DATLKL010000540 DATLKL010000000
VERSION     DATLKL010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74297"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VM713_07245"
     CDS             <1..195
                     /locus_tag="VM713_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84094.1"
                     /translation="APPAAPYPVQAGITLAMRTRALKTNSLDGLSAWAGQSAARAQAR
                     PAAELARDLWSGARALLQPV"
     gene            232..619
                     /gene="rnpB"
                     /locus_tag="VM713_07250"
     ncRNA           232..619
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VM713_07250"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(621..1019)
                     /locus_tag="VM713_07255"
     CDS             complement(621..1019)
                     /locus_tag="VM713_07255"
                     /inference="COORDINATES: protein motif:HMM:NF008769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HVH84095.1"
                     /translation="MTRALPKRPYLLRAMHEWITECGHTPHVIVDAAYEGAEVPRAYV
                     KDGKIVLNLSEGATQRLRLGNEHVEFDARFAGVVHHVRFPVSAVLGVYARETGEGMVF
                     SEQDLGPEPPTRPTPAEEGGPRRPQLKVVK"
     gene            complement(1023..1628)
                     /locus_tag="VM713_07260"
     CDS             complement(1023..1628)
                     /locus_tag="VM713_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017138266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVH84096.1"
                     /translation="MTLFSAPDDPWSHRTRIVLAEKGISIDIVSVEAGRFPEDLLDLN
                     PYHSVPTLVDRDLVLYDSRVIIEYLDERFPHPPLMPVDPLTRAQFRLALYRIERDWYS
                     LAKQIDQEPDKKQAVKLKKILRDTILQSTELFKIKPFFLSDEFSLVDATVAPVLWRLP
                     YYEIDLPPQAQPIEKYMQLVFARPAFREALTDREQEMRLLR"
     gene            complement(1680..2471)
                     /locus_tag="VM713_07265"
     CDS             complement(1680..2471)
                     /locus_tag="VM713_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HVH84097.1"
                     /translation="MRTEAVLVAALLAVAACAPQRLPAAEEESGAKFGADWQSWRADT
                     NVSDLASVQRGARNFVSYCLGCHSLKYERWSRLGADLKIPEPLLAQDLVPPGDKTNEY
                     IQTHLPAADAATWFGKAPPDLSLMVRARGRDYIYQFLTTFYVDPTRQTGANNLRLPTT
                     AMPDVLSELEGLKAAEFRTVARPGRGGSASSEQVFDHFEPLAPGRLSLAEYNGFVRDT
                     VNFLDYVSEPTQLERRQLGVWVVLFLLVFSWLAWLVKREYWKDVR"
     gene            complement(2461..3741)
                     /locus_tag="VM713_07270"
     CDS             complement(2461..3741)
                     /locus_tag="VM713_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009566409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HVH84098.1"
                     /translation="MTVKTATGAAAPMPRTGAADKKGFWGWLNKRLPVDEFLASQVTE
                     YYAPKNFNFWYYFGSLALVVLVLQLVTGIFLTMFYKPGEATAFDSVQFIMREVDWGWL
                     IRYLHSSGASFFFIVVYLHMFRAFLYGSYKPPRELLWLIGMVVYLALMAEAFMGYVLP
                     WGNMSFWGAQVIVNLFGTIPGIGPDLVLWIRGDYGIADATLNRFFSLHVAAIPLALVG
                     LVALHLVALHEVGSNNPDGIEIKEKVGADGRPLDGIPFHPYYTVKDFVGIAVMLVLLA
                     IVVFFVPTLGGLFLEAPNWEPANPMSTPEHIAPVWYFTPYYAILRAVPDQRMGALLML
                     IAVMAFMFVPWLDRSPVKSMRYRGWMSRGALAVFAVSFLALGYLGLQPAEGLYVVLAR
                     IFAALYFAFFFLMPLYTWLDPVKPLPGRVVYHAH"
     gene            complement(3741..4352)
                     /gene="petA"
                     /locus_tag="VM713_07275"
     CDS             complement(3741..4352)
                     /gene="petA"
                     /locus_tag="VM713_07275"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HVH84099.1"
                     /translation="MADHARVAGSDEVDLSRRKFLTRATIATGAVGAVFAAIPFIESW
                     SPSERARAEGAPTELDISKLDAGQMTTTVWRRSPIYVVRRTPDMVARIAGHDAELKDP
                     TSDHSDQPPYAKNPLRSRNPEFLVLVGTCTHLGCLPKQRFAAGELYPSWPGGFFCPCH
                     GSRFDLAGRVFAGSPASINLRVPPYSYPKEHLLRVGADEKGAA"
     gene            4509..5699
                     /locus_tag="VM713_07280"
     CDS             4509..5699
                     /locus_tag="VM713_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVH84100.1"
                     /translation="MLEHLGRGLTFVAGSVVGGFALAFLIVAMRPDLIRGRGTPAAAP
                     SAAPAASAATPAAAPAASTVPAAAPPRTSYADAVQRAAPAVVNVYTDRLVTERLTPSL
                     GELFGEYLPRYRQRIERTLGSGVIVDDKGHIVTNHHVIANAESIRVQLADGRIAEAHV
                     VGSDPDTDLAVLSVGLKFLPVAPLGRSDQLRPGDVVLAIGNPLGLSHTVTHGIVSAVS
                     RAQLGIAPVEDFIQTDAPINRGNSGGALVDSSGALVGINTAMVAKSLGVEGIGFAIPV
                     NLVRGVLSDIIDHGRVIRGWIGISPEDISESQAQQLGLAQGGVVIANLYLGSPAQQAG
                     LQPGDLVLAVDGASPHGAQDVLGRISRHKPGSTVSLHGLRGHRTFDVEARVTERPRAE
                     QREP"
BASE COUNT          989 a         1958 c         2014 g          879 t
ORIGIN      
        1 gcacctccgg cggcgcccta tccggtacag gccggaatta cgctggcgat gcggacacgg
       61 gcgctcaaga ccaacagcct cgacgggctg tcggcgtggg cgggccagtc ggcggcgcgc
      121 gcgcaggcga gacccgccgc ggagctggcc cgggacctgt ggagcggcgc ccgcgcactg
      181 cttcagccgg tttagttgac tttggcggcc gccgccctat cgtgccgggt cagagtcggc
      241 cgggcagtcg ctgctcccct gtcagcaatg gcaagggggt ggaggaaagt ccgggctccg
      301 gcggatcggg tgccaggtaa cgcctggggg gcgcgagccc acggaaagtg caacagagag
      361 cagaccgccc aagtccctcg cgggaccggc aagggtgaaa cggtgcggta agagcgcacc
      421 gcgtcggtgg caacacgcga cggcacggca aaccccacct ggagcaaggc caaataggga
      481 agcggcgtgc gtcttcaccg acggcacgca gcgctgatcc gcgcgcgctt ccgggtaggc
      541 cgctagaggt ccacggcgac gtggatccca gaggaatgat tgccctcgac agaacccggc
      601 ttacaggccg actctttcta ctacttgacg actttcagct gcgggcggcg cgggccgccc
      661 tcctcggccg gtgtcggccg tgtcggcggc tcgggaccca ggtcctgctc cgagaacacc
      721 atgccctcgc cggtctcccg ggcatagacg ccgagcaccg cgctcaccgg aaagcgcacg
      781 tggtgaacga cgccggcgaa gcgcgcgtcg aattccacgt gctcgttgcc gagccgcagc
      841 cgctgcgtgg cgccttcgct gaggttgagg acgatcttgc cgtccttgac gtaggcgcgt
      901 ggcacctcgg cgccctcata cgcggcatcg acgatcacgt gcggcgtgtg gccgcactca
      961 gtgatccact cgtgcatggc acgcagcagg tacggtcgct tgggtaaagc ccgggtcatg
     1021 gcctaacgca gcagcctcat ttcctgctcc cggtcggtga gggcctcgcg gaatgccggg
     1081 cgcgcgaaca cgagctgcat gtacttctcg atcggctgcg cctgcggcgg caggtcgatc
     1141 tcgtaatacg gcagccgcca caggactggc gcgacggtcg cgtccaccag cgagaactcg
     1201 tcggagagga agaagggctt gatcttgaag agctcggtgc tctgcaggat ggtgtcgcgc
     1261 aggattttct tgagcttcac cgcctgcttc ttgtcgggtt cctggtcgat ctgcttggcg
     1321 aggctgtacc agtcgcgctc gatgcggtag agggcgaggc ggaactgcgc gcgcgtgagc
     1381 gggtcgaccg gcatgagcgg cgggtgcggg aagcgctcgt cgaggtattc gatgatgacg
     1441 cgcgagtcgt acagcacgag atcccggtcc acgagcgtgg gcacgctgtg ataggggttg
     1501 aggtcgagca ggtcctcggg aaagcgtccg gcctcgacgc tcacgatatc gatactgatg
     1561 cccttctcgg ccagcacgat gcgcgtccgg tgactccacg ggtcatcggg tgcggagaac
     1621 aaggtcatga tggatcgtct gctcgacacg ctcccgctcc cgcgcccggc gacgcgcggt
     1681 cagcgcacgt ccttccagta ctcgcgcttc accagccagg caagccagct gaagaccagg
     1741 aggaacagca cgacccagac gcctagctgg cggcgctcga gctgggtcgg ctcgctcacg
     1801 tagtcgagga agttcacggt atcgcgtacg aagccattgt actcggcaag gctcaggcgg
     1861 cctggagcga gtggctcgaa gtggtcgaag acctgctcgg agctcgcgct gccgccgcgg
     1921 ccgggccgtg cgacggtcct gaactccgca gccttgagcc cctcgagctc cgacagcacg
     1981 tcgggcatgg ccgtcgtcgg cagccgcagg ttgttggcgc cggtctggcg cgtcggatcg
     2041 acgtagaagg tcgtgaggaa ctggtagatg taatccctgc cccgcgcgcg caccatcagc
     2101 gagagatccg gcggcgcctt gccgaaccag gtcgcggcgt ccgcggctgg caggtgggtc
     2161 tgaatgtatt cgttcgtctt gtcgccaggc ggcacgagat cctgcgcgag cagcggctcg
     2221 gggatcttca ggtccgcgcc gagccgcgac cagcgctcgt atttcagcga gtggcagccg
     2281 aggcagtagc tcacgaagtt gcgcgcgccg cgctgcacgg acgcaaggtc cgacacgttc
     2341 gtgtcagcgc gccagctttg ccagtcggcg ccgaacttcg ccccgctctc ttcctccgcc
     2401 gccggcagcc gctgcggcgc acaggccgcc accgccagca gcgccgcgac aagcacggcc
     2461 tcagtgcgca tggtagacca cccttccagg gagtggcttc accgggtcga gccaggtata
     2521 gagcggcatc aggaagaaga aggcgaagta cagcgcggcg aagatgcgcg ccagcaccac
     2581 gtacagccct tccgctggct gcagtcccag gtacccgagc gcgaggaaac tcaccgcaaa
     2641 gactgccagc gccccgcgcg acatccagcc ccggtagcgc atcgacttca ccggcgagcg
     2701 gtcgagccac ggaacgaaca taaacgccat gaccgcgatc agcatgagga gcgcccccat
     2761 gcgctgatcc ggcaccgcgc gcaggattgc gtagtagggt gtgaagtacc acaccggcgc
     2821 gatgtgttcg ggagtcgaca tgggattcgc cggctcccag ttcggcgcct cgaggaacag
     2881 cccgcccagg gtaggcacga aaaacacgac gatcgcgagg agcaccagca tgactgctat
     2941 gccgacgaaa tccttcaccg tgtaataggg atggaaggga atgccgtcga gcggccggcc
     3001 gtcggcgccc accttctcct tgatctcgat gccgtcgggg ttgttggagc ccacttcgtg
     3061 cagcgccacc aggtgcagcg ccacgagtcc tacgagcgcc agcggtatcg ccgccacgtg
     3121 cagcgagaag aagcggttga gggtcgcgtc ggcgatgcca tagtcgccgc ggatccacag
     3181 aaccaggtcc ggcccgatcc cgggaatggt gccgaagaga ttcacgatca cctgcgcgcc
     3241 ccagaacgac atgttgcccc acggcagcac gtaacccatg aaggcctccg ccatcagcgc
     3301 gagatacacc accatgccga tcagccacag aagctcccgc ggcggcttgt acgagccgta
     3361 gaggaaggcc cggaacatgt gcaggtagac cacgatgaag aagaaggagg cgccgctcga
     3421 gtgcaggtag cggatgagcc agccccagtc gacctcgcgc atgatgaact gcacggagtc
     3481 gaaagccgtc gcctcgcccg gcttgtagaa catggtgagg aagatgccgg tgacgagctg
     3541 cagcaccagt acgaccagcg cgagcgagcc gaagtagtac caaaagttga aattcttcgg
     3601 cgcgtagtac tcggtgacct ggctggccag gaactcatcc accggcaggc gcttgttgag
     3661 ccagccccag aagcccttct tgtcggcagc cccggtcctg ggcatgggag cggccgcgcc
     3721 ggttgcggtc ttcacggtca tcaggcggct cctttctcat cggcgccgac ccgcagcagg
     3781 tgttccttgg gataggagta cgggggcacg cgcagattga ttgaggccgg cgagcccgcg
     3841 aacacccgcc ccgcaaggtc gaagcgcgag ccgtggcagg ggcagaagaa cccgccgggc
     3901 cacgagggat agagctcgcc ggcggcgaac cgctgcttgg ggaggcagcc gaggtgggtg
     3961 caggtgccga ccagcaccag gaattccggg ttgcgcgagc gcagcggatt cttggcgtag
     4021 ggaggctgat cggagtgatc cgaggtcgga tccttcagct cggcgtcgtg gccggcaatg
     4081 cgcgccacca tgtcgggggt gcgccgcacg acgtagatcg gcgagcgacg ccatacggtc
     4141 gtggtcatct gcccggcatc gagcttggag atgtcgagct cggtcggcgc gccctcggcg
     4201 cgggcgcgct ccgacggcga ccaggactcg atgaagggga tggcggcgaa cacggcgccc
     4261 accgcgccgg tggctatggt ggctctggtg aggaacttgc gccggctcag gtcgacctcg
     4321 tcgctgccgg cgacgcgtgc gtgatccgcc atctcaccac tctcccgctg tgtgacacgc
     4381 ccgccgcagc ttcgtggcgc atcgctcacc aggcggactg cgcatggtgc agtttcgtac
     4441 cttgcggggg gcggcgcatt atacacaaac gaacatgcct ccccggcgcg gtacgcacgg
     4501 agttgtgaat gctggaacac ctgggccgcg ggctgacgtt cgtggccggt tcggtcgtcg
     4561 gcggattcgc cctcgccttc ctgatcgtgg cgatgcggcc ggacctcatc cgtgggcgcg
     4621 gtacgcccgc ggccgcgccc agcgccgccc cggccgcgag tgctgccaca cccgccgccg
     4681 ctccagccgc gtccacagtg cccgcggccg cgccgccccg cacctcttat gccgacgcgg
     4741 tgcagcgcgc ggcgccggcg gtggtcaacg tatacacgga ccggctggtc accgagcggc
     4801 tgactccatc gctcggggag ctgttcggcg agtacttgcc gcgttaccgg cagcgcatcg
     4861 agcgcacgct cggctcgggc gtcatcgtcg acgacaaggg acacatcgtc accaatcacc
     4921 acgtcatcgc gaacgccgag tcgatccgcg tccagctcgc cgacggccgc atcgccgagg
     4981 cgcacgtcgt cggcagcgac ccggacaccg acctcgcggt gctcagcgtg gggctgaagt
     5041 tcctgccggt ggcgccgctc gggcgctccg atcagctgcg cccgggcgac gtcgtgctcg
     5101 ccatcggcaa tccgctcggg ctctcacaca cggtgacaca cggcatagtg agcgccgtga
     5161 gccgcgcgca gctcggcatc gcgccggtcg aggacttcat ccagacggac gccccgatca
     5221 accgcggcaa ctccggcggg gcgctggtcg actcgagcgg cgcgctggtc ggcatcaaca
     5281 ccgcgatggt cgcgaagagc ctgggcgtcg agggcatcgg ctttgccatc ccggtaaacc
     5341 tcgtgcgcgg ggtgctgagc gatatcatcg accacggccg cgtgatccgc ggctggatcg
     5401 gaatctcgcc cgaggacatc tccgagagcc aggcccagca gctcgggctg gcccagggcg
     5461 gcgtggtgat cgccaacctc tatcttggaa gcccggcgca gcaggcaggc ctccagcccg
     5521 gcgacctggt gctcgcggtg gatggggcga gtccgcacgg cgcgcaggat gtcctcgggc
     5581 gcatctcccg gcacaagccc gggtcgactg tgagcctcca tggcctgcgc ggccaccgaa
     5641 ccttcgacgt cgaggcgcgc gtgactgagc gtccccgcgc ggagcagcgc gagccgtgac
     5701 cgagctcacg acccggcgct tcgcctcacg caccgcgctc gagtccgcgc tcgaggagcg
     5761 cctggcacgg gcgatcgccg cccccgccgc cgcggcgctc atgctctcgg gcgggaccac
     5821 cccgctaccc gtgtaccgcg
//
LOCUS       DATLKL010000541         1862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_130648, whole genome shotgun sequence.
ACCESSION   DATLKL010000541 DATLKL010000000
VERSION     DATLKL010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130648"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VM713_07285"
     CDS             complement(<1..387)
                     /locus_tag="VM713_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVH84101.1"
                     /translation="MSESLAVVLIPGLLDSARLYAAQIPELWRRGPVTVADHTRDDSM
                     REIARRILGSAPARFALVGLSMGGYISFEILRQAPARVAKLALLDTSARPDTPEATAM
                     RRTQMTFASNGRLGEVVETQFERMVHP"
     gene            411..1580
                     /locus_tag="VM713_07290"
     CDS             411..1580
                     /locus_tag="VM713_07290"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HVH84102.1"
                     /translation="MSHRIAHLVVALVVTASTAAPAAAPPAAPGAALAPDAAARFAAL
                     ALTCLHQEYPNHISHTLNSAADSRPPHELTPAFYGCLDWHSDVHGHWLLVRLLRLFPD
                     APFAAAARAALEQSFTAEHIDAEAGYLHGAGRASFERPYGLAWLLELAAELRRFDDPD
                     AQRWSAVLAPLETEAVARLTSWLPKLEYPIRIGEHDQTAFAFGLMWDWAGVAGDPGMR
                     RLLADAAERFYRRDRNCPLAYEPSGEDFLSPCLAEADFMRRVLPTAAFASWLSAFLPQ
                     IPRSAPAAPSLAKARAWLEPGIVTDRSDPKLAHIDGLNLSRAWMLEGIAHGLPPGDGR
                     IPALRAAAARHASAALPAVSGGYYKGGHWLGTFAVYLTSRAGLEQVDAPPAAAAH"
     gene            complement(1581..>1862)
                     /locus_tag="VM713_07295"
     CDS             complement(1581..>1862)
                     /locus_tag="VM713_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84103.1"
                     /translation="ADVLSLQGRGRLRAGEFADVVIFDPAKFQDHATYQKPMQYATGV
                     KDVFINGALALKDGEPTGAHTGRILRGRAWTGAPGGGCRQSSKDWTWID"
BASE COUNT          261 a          697 c          626 g          278 t
ORIGIN      
        1 cggatgcacc atgcgctcga actgcgtctc caccacttcg ccgagccgcc cgtttgaggc
       61 gaacgtcatc tgcgtgcggc gcatcgcagt cgcctcgggg gtatcggggc gcgcggaggt
      121 gtcgagcagc gccagcttgg cgacacgcgc cggtgcctgg cgcaggatct cgaaggagat
      181 atagccgccc atcgacagcc cgacgagcgc gaaacgcgct ggcgcgctgc cgagaatccg
      241 gcgcgcgatc tcgcgcatgc tgtcgtcgcg ggtgtgatcg gcgaccgtca ccgggccgcg
      301 ccgccacagc tcggggatct gggcggcgta aaggcgcgcc gagtcgagca atcccgggat
      361 caacacgaca gcaagagatt cgctcacgca cgcgagcgta acatgcgtcc atgagccacc
      421 gtatcgcgca cctcgtcgtt gcgctcgtag tgactgcgtc caccgcggcg ccggcagcgg
      481 cgccacccgc tgcgcccggg gcggcgctgg cgcctgatgc ggcggcacgc ttcgcggcgc
      541 tcgcgctcac gtgcctgcac caggagtacc cgaaccacat cagccacacc ctgaacagcg
      601 ccgccgattc ccgcccgccg cacgagctga cgccggcctt ctacggctgt ctcgactggc
      661 attcggacgt gcacggccac tggctgctgg tgcggctgct gcggctcttc ccggacgcgc
      721 cattcgccgc cgcggcgcgc gccgcactcg agcagagctt cacggccgag cacatcgacg
      781 ccgaggcggg ctacctgcac ggcgcgggcc gcgcctcctt cgagcggccc tatggacttg
      841 cctggctgct cgagctcgcg gcggagctgc gccgcttcga cgatcccgat gcgcagcgct
      901 ggtccgccgt gcttgcgccg ctcgagaccg aggcggtggc gcgccttacg agctggctgc
      961 cgaagctcga gtacccgatc cgcatcggcg agcacgacca gacggcattt gccttcgggc
     1021 tcatgtggga ctgggccgga gtggcgggcg atcccgggat gcgccggctg ctcgcggatg
     1081 cggccgagcg cttctaccgc cgcgatcgca actgtccgct cgcctacgag ccttcgggcg
     1141 aggatttcct ctcgccctgc cttgcggaag ccgacttcat gcggcgcgtg ctgcccacgg
     1201 ccgccttcgc gtcctggctg tcagcgttcc tgccgcagat cccgcgcagc gcaccggcgg
     1261 cgccctcgct cgccaaggcg cgcgcctggc tcgagcccgg catcgtcacc gaccgctcgg
     1321 atcccaagct cgcgcacatc gacgggctca acctgagccg tgcctggatg ctcgaaggca
     1381 tcgcccacgg cctgccgccc ggcgacgggc gcatcccggc gctgcgcgcc gcggcggcac
     1441 gtcatgccag cgccgcactg ccggcggtca gcggggggta ctacaagggc ggccactggc
     1501 tcggaacctt tgccgtgtac ctcaccagcc gcgcgggact cgagcaggtc gacgcgccgc
     1561 cagccgcggc cgcgcactga tcagtcgatc caggtccaat ccttgctcga ttgccggcag
     1621 ccgcctccgg gcgccccggt ccatgcgcga ccccgcagga tgcgcccggt gtgtgcaccg
     1681 gtcggctcgc cgtccttgag agccagagcg ccgttgatga agacgtcctt gaccccggtc
     1741 gcgtactgca tcggcttctg ataggtcgcg tgatcctgga acttcgccgg atcgaagatg
     1801 acgacgtcgg cgaactcacc ggcccgcagc cgcccgcgac cttgaagtga cagcacgtcc
     1861 gc
//
LOCUS       DATLKL010000542         1661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_62849, whole genome shotgun sequence.
ACCESSION   DATLKL010000542 DATLKL010000000
VERSION     DATLKL010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62849"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VM713_07300"
     CDS             <1..851
                     /locus_tag="VM713_07300"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF010006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HVH84104.1"
                     /translation="QNGAYCQRIARMCRVLGRKLTTIDYAENMQVDTADVDRALRTDA
                     SITHVALVHCETSTGILNPLKEVAEVVARHGRGLIVDAMSSFGALEIDARTTPFDAVV
                     AASGKCLEGPPGVGFVVVRREALERCEGNCHSLSLDLYDQWIYMQKTTQWRYTPPTHV
                     VAAFDAAIAQYLEEGGLAARAARYAQNCRTLIGGMSKLGLKSFLPAAIQAPIIVTFFA
                     PDSPRYVFKDFYNAVKARGYILYPGKLTTVETFRVGCMGQLGERGIAGAVEAVGKVLA
                     EMRAAA"
     gene            848..>1661
                     /locus_tag="VM713_07305"
     CDS             848..>1661
                     /locus_tag="VM713_07305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH84105.1"
                     /translation="MKGPSTAAAPATRALRVAQARTVVLLFLGYAACYFCRSNLSVAT
                     PLLIDELGRHGISHDDAIVAIGSMASFGVFAYALGKWFLTSLGDYWGGKRNLLIATGG
                     ATAFTLLFAASAALPMFTLAWIGNRLSQSIGWSALVKVSSKWFHYSRYGTVIAILTCS
                     YLLGDAGARQSMSVLLERGVGWRGLFLFGAAVAGAMFLANLLLLRESCTDEGHPEAVA
                     NPRNVFAAAQARPQSYLTLVLPLLKTRAFLMVCVLGFATTMVRETFNTWTPVY"
BASE COUNT          255 a          584 c          528 g          294 t
ORIGIN      
        1 cgcaaaacgg ggcttactgt cagcgcatcg cccgcatgtg ccgcgtgctc ggtcgcaagc
       61 tgacgaccat cgattacgcc gagaacatgc aggttgacac cgcagacgtc gaccgcgcgc
      121 tgcgcaccga cgcctcgatc acgcacgtgg cgctggtgca ttgtgagacc agcaccggga
      181 tcctgaaccc cctgaaggaa gtcgccgagg tcgtcgcgcg ccacggccgg ggcctcatcg
      241 tcgacgcgat gagctccttc ggcgcgctcg agatcgacgc gcgcaccacg cccttcgatg
      301 ccgtcgtagc agcttcgggg aaatgtctcg agggaccgcc cggcgtgggc ttcgtcgtcg
      361 tgcgccgcga ggcgctcgag cgctgcgagg gcaactgcca ttcgctctcg ctcgacctgt
      421 atgaccaatg gatctacatg cagaaaacca cccaatggcg ctacacgccg ccgacccacg
      481 tcgtggctgc attcgatgcg gcaatcgccc agtatctcga ggagggcggc ctcgccgcgc
      541 gcgccgcccg ctatgcgcag aactgccgca ctctgatcgg cggcatgtcc aaactcgggc
      601 tgaagagctt tctgcccgca gccattcagg cgccgatcat cgtgaccttc ttcgcgcccg
      661 acagtccccg ctacgtcttc aaggatttct acaacgccgt caaggcgcgc gggtatatcc
      721 tgtatccggg caagctcacc acggtggaga cgttccgggt cggctgcatg ggacagctcg
      781 gcgagcgcgg catcgccggc gcagtcgagg cagtaggcaa ggtgctcgcg gagatgcggg
      841 ccgcggcttg aagggcccca gcaccgccgc cgcgcccgcc acgcgcgcgc tgcgcgtcgc
      901 gcaggcgcgt accgtggtac tgctattcct cggctacgcg gcctgctact tctgccgctc
      961 gaacctctcg gtggcgacgc cgctcctgat cgatgagctc ggccggcacg gcatcagcca
     1021 cgacgacgcc atcgtcgcca tcggctcgat ggcctccttt ggtgtcttcg cttacgcgct
     1081 cggcaagtgg tttctcacga gtctcggcga ctactggggc ggcaagcgca acctcctcat
     1141 tgcaaccggc ggcgcgaccg ccttcacgct gctgttcgcc gccagcgccg cactgccgat
     1201 gttcacgctc gcctggatcg gcaaccgcct gtcgcaatcg atcggctggt cggcactcgt
     1261 caaggtctcg tccaagtggt ttcactattc gcgctatggc acggtgatcg ccatcctcac
     1321 ctgcagctac ctgctcgggg acgcgggcgc acggcagagc atgagcgtgc tgctcgagcg
     1381 gggcgtcggc tggcgcgggc tgttcctgtt cggcgcggcg gtggccggcg cgatgttcct
     1441 cgcgaatctg ctcttgctgc gcgagtcgtg caccgacgag ggccacccgg aagcggtcgc
     1501 caatccgcgc aacgtgttcg ccgccgcgca agcgcgcccg cagagctacc tgacgctggt
     1561 gctgcccctg ctcaagaccc gcgcatttct catggtgtgt gtgctggggt tcgccaccac
     1621 catggtgcgc gagaccttca acacctggac gccggtgtac t
//
LOCUS       DATLKL010000543         1840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_209423, whole genome shotgun sequence.
ACCESSION   DATLKL010000543 DATLKL010000000
VERSION     DATLKL010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209423"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VM713_07310"
     CDS             <1..550
                     /locus_tag="VM713_07310"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HVH84106.1"
                     /translation="PTLVYHESEPGHHFQLALVLEMPKLPLIRKAGGGFSANTEGWAV
                     YAEQLCAEMGMYDSEPLGRLGMLQSLLFRAARCVVDTGIHAKGWSRERAIDYMVETAG
                     EVRSGMTTEVERYCTWPGQATSYKVGQTRWLKLRAAARARLGPKFDIRDFHDAGLSAA
                     PMPLAVLERVIDDWVTSRLKGA"
     gene            553..1620
                     /locus_tag="VM713_07315"
     CDS             553..1620
                     /locus_tag="VM713_07315"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVH84107.1"
                     /translation="MRNHSLACLIGAALLAMQAAAAYPAYEGDYVIRDFKFASGESLP
                     ELRIHYRTFGAPRADAHGIVRNAVLIMHGTGGSGAQFTQPMFAGELFGPGQPLDAQRY
                     FIVLPDGIGHGASAKPSDGLHARFPHYGYNDMVQADYLLLTQGLRVNHARLVMGTSMG
                     GMHTWLWGEQHPEFMDALMPLASLPTQISGRNRVWRRTLIDAIRNDPAWEGGEYASEP
                     PALRTDIEMLWLMGSNPVLRQAEAPTLARADEVIEAYVAENLKTYDANDILYALEASH
                     DYDPGPGLERIQAPLLAINSADDLINPPELGILEREIKRVPQGRAVVIPYSERTRGHG
                     SHTVAALWKDDLIGLLKSPER"
     gene            complement(1625..>1840)
                     /locus_tag="VM713_07320"
     CDS             complement(1625..>1840)
                     /locus_tag="VM713_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HVH84108.1"
                     /translation="GFRRGTDIIKALALGAEAVMLGRAPLYGLAAGGEPGVVRALEML
                     YTETDRALGMLGCNSVSELGPQHVRRA"
BASE COUNT          302 a          606 c          640 g          292 t
ORIGIN      
        1 gccgacgctg gtctaccacg agtcagagcc cggccatcat ttccagctgg ccctcgtgct
       61 ggagatgccg aagctccctt tgatccgcaa agccggcggc ggcttctcgg ccaacaccga
      121 aggctgggcg gtgtacgcgg agcagctgtg cgccgagatg ggaatgtacg actcggagcc
      181 gctcgggagg ctcgggatgc tgcagtcgct gctgttccgt gccgcgcgct gcgtcgtcga
      241 taccggcatt cacgccaagg ggtggagccg cgagcgcgcg atcgactaca tggtggagac
      301 cgccggcgag gttcgcagcg gcatgacgac cgaggtagag cgctactgca cctggccggg
      361 tcaggcgacc agctacaagg tcggccagac tcgctggctg aagctgcggg ccgcggcgcg
      421 cgcgcggctg ggacctaagt tcgacattcg tgacttccac gatgcgggac tctcggcggc
      481 gcccatgccg ctcgcggtgc tcgagcgcgt gatcgacgac tgggtgacga gccgtctcaa
      541 gggagcctga gcgtgagaaa tcactcgctg gcgtgcctga taggggctgc gcttctggcc
      601 atgcaggcgg ccgcggctta cccggcgtac gagggcgact acgtcattcg cgacttcaag
      661 tttgcctccg gagagtcgct cccggagctg cgcatccact accgcacgtt cggcgctccg
      721 cgcgccgacg ctcacggcat cgtgcgtaat gcggtgctca tcatgcacgg cacgggcggg
      781 agcggggcac agttcacaca accgatgttc gccggcgagc tcttcggccc ggggcagccg
      841 ctcgatgcgc agcgttactt catcgtgctg ccggacggta tcggtcatgg cgcatctgct
      901 aagccgagcg acggcctgca cgcgcgcttt ccgcactacg gctacaacga tatggtacag
      961 gcggactatc tgctgctcac gcagggactc agggtgaatc acgcgcgcct cgtgatgggc
     1021 acctcgatgg gcggcatgca cacctggctg tggggcgagc agcatcccga gttcatggat
     1081 gcgctgatgc cgctcgcgag cttgccgacg cagatctccg gtcgcaatcg ggtgtggcgg
     1141 cgcacgctga tcgatgcgat ccgcaacgat ccggcatggg agggcggcga gtacgcgagc
     1201 gagccgcccg cgctccgtac cgatatcgag atgctgtggc tgatggggag caatcccgtg
     1261 ctgcggcagg cggaggcgcc gacgctggca cgcgcggacg aagtgatcga ggcgtacgtc
     1321 gcggagaacc tcaagaccta cgacgccaac gacatcctgt acgcgctcga agcctcgcac
     1381 gactacgatc cggggccggg cctcgagagg attcaggcgc cgctgctggc gatcaactcc
     1441 gccgatgatc tgatcaatcc gccggagctc ggcatcctcg agcgggagat caagcgtgtg
     1501 ccgcagggcc gcgccgtcgt gatcccctac agcgagcgca cccgcggcca cgggtcgcac
     1561 accgtcgcgg cattgtggaa ggacgatctc atcgggctgc tgaagtctcc cgagcgctga
     1621 ggcgtcacgc ccgccgcaca tgctgcgggc cgagctcgct caccgagttg catcccagca
     1681 tgccgagcgc ccgatcggtc tcggtgtaga gcatttcgag cgcgcgcacg acgccgggct
     1741 cgcccccggc agcgaggccg tagagcggcg cgcgcccgag catcaccgcc tcggcgccga
     1801 gcgccagtgc cttgatgatg tcggtgccgc ggcggaagcc
//
LOCUS       DATLKL010000544         3054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_187064, whole genome shotgun sequence.
ACCESSION   DATLKL010000544 DATLKL010000000
VERSION     DATLKL010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187064"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..>3054
                     /locus_tag="VM713_07325"
     CDS             56..>3054
                     /locus_tag="VM713_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84109.1"
                     /translation="MNAGAALRLLERSRGMSAQDANQLQLERTAWLDFSGAGLTVVDH
                     IAGEMRQGWRLEMSPPFVLESVRTASDKSLLVTSGMAPGATGVEVRQPEVDLTTAARL
                     PDAGGRLPATGWRQRFTNASGQLIVGPGYRLLVALGPDSAPQAWLERWRLLDIFAVLL
                     IATAAGRLLGRRVAAIAVAAVALTYQELGAPAWLWVNVLVALALVRVVPEGRLGRLVA
                     AYRVLAVALLLVALVPFLIGQARLAVYPQLETYLSAGQGAAASSYGRVAAVNAPVEAK
                     LAAPPIQTQIVEEAKKQRLPRAPAASLSATSVSRAAVLVQEVAVTATRRESSAGYEPG
                     VLVQAGPGLPQWRYHVYDYSWSGPLEPNATARFVISPPWLTGLWRIAGIVLSVLLIVE
                     LTRGDIERLPDWLRRPWLAPTAVLLLTAAAALGIAPEARADTTPAPALLKELESRLLE
                     PPKCAPDCADVIEAHVSVDASRLQVALTVAALDAVGIALPGAEPNWAPDTVLVDGATV
                     GWVYRSAAGVRYVSVPPGRHAVRIAGSLEGLEGLALAFPLAPHVIDVSAAGWDVSGVT
                     ARRLVSGALELVRRRAAQESGGAARQEEFPPFVRVTRIFRLAHDWTIDTVIERVAPPS
                     SAFTVRLPLLPRESVTSAGLEAPDNRVTVGLAANEQQTEFSSIIPVSDTLELLAPESA
                     PYGERWVFAVGSMWHVDLSGVPPVAPGDDTGGTYEYYPRPGEHLAVRITRPQPAAGGT
                     LAYDGAELMLKAGKRSSDLSLVLSYRSTQGGRQVLHLPDDVEVTLVRSDNDTLGLRPE
                     KGELSLPALPGRHTWTVNWRSAAGAGLVTRAPVVELSAPASNLHIGMRIPEDRWVLLA
                     FGPGIGPAILYWGELVVFIAVAWLIGRSRLTPLPVRDWLLLGLGLSTFSWSVFGLFVL
                     FVALFQWRARSAPLADGRRFNLLQAGLAVLAVAAVLCVLAAVPGGLLARPDMRISSGG
                     GDYGALEWFVDQTRGALPRPGVLSVSL"
BASE COUNT          404 a         1026 c         1101 g          523 t
ORIGIN      
        1 ggacccgacc caggccaacg tgccctccga ttggcgcgga ctgcccgcct atcgcatgaa
       61 cgcaggcgcg gcgctgaggc tcctcgagcg cagtcgcggc atgtctgcgc aggatgccaa
      121 ccagctacaa ctcgagcgca cggcctggct ggatttctcg ggcgccggtc tcacggtcgt
      181 cgaccacatc gccggcgaga tgcgccaggg gtggcggctc gagatgagtc cgccgtttgt
      241 gctggagagt gtgcgcacgg catcggataa atcgctgctc gtgacctccg gtatggcacc
      301 cggcgcgacg ggcgtcgaag tgcggcagcc cgaggtggat ctgaccaccg ccgcgcgcct
      361 gccggacgcg gggggtaggc tgcccgccac cggctggcgc cagcgcttca ccaacgcgtc
      421 gggacagctc atcgttgggc ccggttaccg tctactggtg gccctcggac ccgactcggc
      481 tccgcaggca tggctcgagc gttggcggct gctcgacatc ttcgcggtgc tgctcatcgc
      541 gaccgccgcg gggcggctgc tcggccggcg cgtcgcggcc atcgctgtcg ccgcggtggc
      601 cctgacgtat caggaactgg gtgctccggc atggctgtgg gtgaacgtgt tggtggcact
      661 ggcactcgtg cgggtggtcc cggaaggaag gctgggtcgc ctggtcgctg cataccgtgt
      721 gctcgcagta gcgctgctct tagtcgcgct ggtgcccttc ctcatcgggc aagcgcgtct
      781 tgccgtctat ccgcaacttg agacctatct ctcggccggg cagggcgccg ctgcgtccag
      841 ctacgggcga gtcgcggcgg taaatgcccc cgtcgaagca aaactcgccg ctccgccgat
      901 tcagacgcag attgtcgagg aggcgaagaa gcaacggttg ccgcgggcgc ctgccgcgtc
      961 gctgagtgca accagcgttt cccgggcggc tgtcctcgtc caggaggttg cagttactgc
     1021 cacgcgccgc gaatcgtccg ccggctacga gccgggggta ctcgtgcagg ccggccccgg
     1081 cctgccgcaa tggcgctacc acgtctatga ctacagctgg agcggcccgc ttgagccgaa
     1141 cgccaccgcg cgcttcgtga tcagtccgcc gtggcttaca gggctgtggc gcatcgcggg
     1201 catcgtcctg tcggttctgc tcatcgtcga gctgacgagg ggtgacatcg aacggcttcc
     1261 cgactggctg cgtcgcccgt ggctcgctcc gaccgctgtg ctgcttttga ccgcggcggc
     1321 ggcgcttggc atcgcgccgg aggcgcgcgc cgacaccacg ccggctccgg ccctgcttaa
     1381 ggaattagag tcgcggctgc tcgaaccgcc gaaatgcgcg cccgactgtg cggacgtgat
     1441 cgaagcgcat gtgagcgtgg atgcgtcgcg tcttcaggtc gcactcacgg tggctgcgct
     1501 ggacgcggtc ggtatcgcct tgccgggtgc cgagccgaac tgggcgccgg acacggtgct
     1561 ggttgacggc gcgacggtgg gctgggttta ccggagtgct gcaggcgtgc gctacgtgag
     1621 cgttccgccg gggcgccacg cggtgcggat cgcaggctcg ctcgaggggc tcgaggggct
     1681 cgcgctcgcg tttccgctcg cgccgcacgt cattgacgtg agcgccgcgg gctgggacgt
     1741 ctcgggtgtg actgcgcgac gcctggtgag cggggcactc gagctcgtgc gccggcgcgc
     1801 tgcgcaagag agcggcggcg ctgcgcgcca ggaggagttc ccgccgttcg tcagggtcac
     1861 tcggatcttc cgcctggcgc acgactggac catcgacacc gtcatcgagc gcgtggcgcc
     1921 gccgtccagt gccttcacgg tgcgcctccc gctcctgcca cgggagtcgg tcacgagcgc
     1981 cgggctcgaa gcgcccgaca accgtgtgac ggtcggcctg gcggccaacg agcagcaaac
     2041 cgagttcagc tccatcattc cagtcagcga cacgctcgag ctgctcgcac ccgaaagcgc
     2101 tccctacggt gagcgctggg tgttcgcggt cggctcgatg tggcacgtgg acctcagcgg
     2161 ggtgccgccg gtcgcgccgg gcgacgacac gggcggaacc tatgagtact acccgcggcc
     2221 aggcgagcac cttgcggtca ggatcacccg cccgcagcca gccgcaggag ggacgctcgc
     2281 ttacgacggc gcggagctga tgctcaaagc aggcaagcgc tcgagtgact tgagcctggt
     2341 gctgtcatac cgcagcacac agggtgggcg acaggtgctg cacttaccgg acgacgtcga
     2401 ggtcacgctg gtgcgcagcg acaacgatac actcgggctg cggccggaga agggcgagct
     2461 gtcgctgccg gcgctgccgg gccgccacac ctggacggtc aactggcgca gcgcggcggg
     2521 cgcgggcctc gtgacacgtg cccccgtagt ggagctgtcg gcgccggcca gcaaccttca
     2581 tatcggcatg cgcattcccg aggaccgctg ggtgctcctc gccttcggcc caggcatcgg
     2641 accggcgatc ctctactggg gcgagctggt cgtgttcatc gccgttgcct ggctcatcgg
     2701 gcgctcgcgc ctcacgccgc taccagtgcg cgactggctg ctgctcgggc tggggctatc
     2761 gaccttctcg tggtcggtat tcggcctgtt cgtgctcttc gtggcgctgt tccaatggcg
     2821 tgcccgcagc gctccgctcg cggatgggcg ccgcttcaac ctgctgcagg cgggacttgc
     2881 agtgctagcg gtggccgcag tgctgtgcgt gttggcggcg gtgccgggcg ggttgctcgc
     2941 gcggccggac atgcgcatta gcagcggcgg cggcgattac ggcgcgctcg agtggttcgt
     3001 cgaccagacg cgcggtgcgc tgccgcgccc cggcgtgctg tcggtctctc tatg
//
LOCUS       DATLKL010000545         2027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_175527, whole genome shotgun sequence.
ACCESSION   DATLKL010000545 DATLKL010000000
VERSION     DATLKL010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175527"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VM713_07330"
     CDS             <1..781
                     /locus_tag="VM713_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVH84110.1"
                     /translation="AAALLVRLKTRRLAGAASRAERLARIAAEKSDWEAELAGWTHER
                     DPYSLEVSSDSKYMHPRQMLRALERALPGGSMVSTDIGNICSVANSYLRFDQPRSMFA
                     AMSFGNCGYAFPVICGAKVAAPGRPAFAYVGDGAWGISFNELLTCAREKIGVTPIVFN
                     NGQWGAEKKNHVDFYSRRYQGVNLENPSWARAAQAFGCEGVTVERLTDIEPALRAAVA
                     AQAEGRTTVLEMMVTQELGDPFRRDALSKPVRHLAKYQKFV"
     gene            819..>2027
                     /locus_tag="VM713_07335"
     CDS             819..>2027
                     /locus_tag="VM713_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVH84111.1"
                     /translation="MSGDAAPPELAASGLRRSLQRRHMQMIALGGVIGAGLFVGSGVV
                     IASAGPAAVISFALTGTLVVLVMRMLGEMAVAYPAIGGFYEYNRLALGELAGFLTGWM
                     YWYFWVIVVALEAVAGAKILGTWWPQVAPWEFTLALMTLFTIVNLLSVRSYGEAEFWF
                     ASIKVAAIVAFLVAGALFVFGGWPGAHAGLPHLTADGGFMPNGIVPVLTGAVAATGFY
                     FGAEIVTIAAAESAEPERAVAETTQSVIWRVLVFYIGSIFLVVALVPWNDAVRMSRPY
                     VSVMEVLRIPAAATLMSLVILTAVLSALNSGLFASSRMLMALAGRGDAPAAAARLDRR
                     GVPVAAILASTAFGYATVVMSYLSPDRIFAFLVNSYGTVAIFVYVLIAVAELRLRRRL
                     EREAPERLRIR"
BASE COUNT          271 a          705 c          696 g          355 t
ORIGIN      
        1 ggccgctgcg ctcctcgtgc gcctcaagac ccgcaggctc gccggggccg ccagccgcgc
       61 cgagaggctg gcgcgcatcg cggcggagaa atccgactgg gaggcggagc tcgccggctg
      121 gacgcacgag cgcgatccgt attccctcga ggtctcatcc gactcgaaat acatgcaccc
      181 gcggcagatg ctgcgcgcac tcgagcgggc gctccccggg ggctccatgg tgtcgaccga
      241 catcggcaac atctgctcgg tggccaactc ttacctgcgg ttcgaccagc cgcgctcgat
      301 gttcgccgcg atgagcttcg gcaactgcgg ctacgcgttc ccggtgatct gcggcgccaa
      361 ggtcgccgcc cccgggcgcc cggcgttcgc ctacgtgggc gacggcgcct ggggcatcag
      421 cttcaacgag ctcctcacct gcgcgcgcga gaagatcggc gtgacgccga tcgtcttcaa
      481 caacggccag tggggcgccg agaagaagaa tcacgtcgac ttctattcgc gccgttacca
      541 gggagtgaac ctggagaatc cgagctgggc aagagccgcg caggccttcg gctgcgaagg
      601 cgtcacggtc gagcgactca ccgacatcga gccggcgttg cgcgccgccg tggcggcgca
      661 ggccgaaggg cgcaccaccg tgctcgagat gatggtcacc caggagctcg gcgacccgtt
      721 ccgccgcgat gcgctctcga agccggtgcg gcacctcgcc aagtaccaga agttcgtgtg
      781 agcacctccg cgtgcaccgg atcgaaggtg ctgcgccgat gagcggggac gcagcgccgc
      841 cggagcttgc cgcgagcggc ctgcgccgct cgctgcagcg ccgtcacatg cagatgatcg
      901 cgctcggcgg cgtaatcggc gccggcctct tcgtcggcag cggcgtcgtc atcgcctcgg
      961 cgggacctgc agcggtcatc tccttcgccc tcaccggcac tctcgtcgtg ctggtgatgc
     1021 gcatgctggg cgagatggcg gtggcctacc ccgcgatcgg cggcttctac gaatacaacc
     1081 gcctggcgct cggggagctc gcgggattcc tcacgggctg gatgtactgg tacttctggg
     1141 tgatcgtcgt cgcgctcgaa gcggtcgcgg gagcgaaaat cctcggcacc tggtggccgc
     1201 aggtggcgcc ctgggagttc accctcgcgc tgatgacgct gttcaccatc gtgaacctgc
     1261 tctcggtccg ctcctacggc gaagcggagt tctggtttgc ctcgatcaag gtggcggcca
     1321 tcgtggcgtt tctcgtcgcc ggcgcgctgt tcgtctttgg cggctggccc ggggcgcacg
     1381 cgggcttacc gcacctgacg gccgacggcg gcttcatgcc gaatggcatc gtaccggtgc
     1441 tcacgggagc ggttgccgcg accggcttct acttcggcgc cgagatcgtc acgatcgccg
     1501 ccgccgaatc cgccgagccg gagcgggcgg tcgcggagac cacgcagtcg gtgatctggc
     1561 gcgtgctggt cttctacatc ggctcgatct tcctggtcgt cgccctcgtg ccctggaacg
     1621 acgcggtgcg catgtcgcgc ccctacgtga gcgttatgga ggtgctgcgc attccggccg
     1681 ccgctacgtt gatgagcctc gtgatcctca ccgcggtgct ctcggcgttg aactccggcc
     1741 tgttcgcctc ttcacgcatg ctgatggctc tcgccgggcg gggcgatgcg cccgccgccg
     1801 ccgcgcggct cgaccggcgc ggcgtgccgg tggccgccat tctcgcgagc accgccttcg
     1861 gctacgcgac ggtggtgatg agctacctct cccccgaccg gatcttcgcc tttctcgtca
     1921 actcctacgg caccgtggcg atcttcgtct acgtgctgat cgcggtggcg gagctgcggc
     1981 tgcggcgccg tctggagcgc gaggcgccgg agcggctgcg catccgc
//
LOCUS       DATLKL010000546         2527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_17627, whole genome shotgun sequence.
ACCESSION   DATLKL010000546 DATLKL010000000
VERSION     DATLKL010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17627"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1718
                     /locus_tag="VM713_07340"
     CDS             <1..1718
                     /locus_tag="VM713_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH84112.1"
                     /translation="PAIIAGFGAGLPLVLLGAWLFELTPEGFKLTSSVDRHRSIRELT
                     ARKLDVIIAILFALAVVAMLADRWIFHRRPDESVVPIFIVVFLVLLADRVIGRGGPPG
                     ATRAAAAPLTTGATAPPSIAVMPFVDMSEQKDQEHFSDGLSEELITLLTKVADVRVPA
                     RTSCFYFKGRQCTVAEIAAALHVEHLLEGSVRKSGDKVRITVQLIRVDSGYHVWAETY
                     DRKLDDIFEVQAQIATAVVRALKATLLSATLPARLGLHSSQAYELYLLGRFHWNKRSP
                     DEFRKAIECFRQAIVAQPNYPLAFCGIADSYSLLPIYDSAVQATETMPQAKQAALKAL
                     SLDEGLAEAHASLALTLALFDYDWAAAERHLTRAIELNPNSPSPHQWYCELLVNTGRL
                     EEGLEEGRLSVHIDPLSLVANLGLGIALNSARRYDEAIAQLQKTLELDARFADASYFL
                     FEAYANQGKYREAVEVYARQKSLDGEPAAEITAMQQAFTEHSWEGFLRHRISFLEAQP
                     HPIAEEVAALCARVGDQERACAWLEKSYEGRSARLTHLKVDARYDKLRSNPRFARLVH
                     RIGLS"
     gene            1802..>2527
                     /locus_tag="VM713_07345"
     CDS             1802..>2527
                     /locus_tag="VM713_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVH84113.1"
                     /translation="MTPGSVVVPVYVWLTPALIALFLYGLGQGLVKKWIGEVSPARFC
                     LYYVLATSIVNFGFFLTQDHPPLLAASGTGFLVAGVLAYVFDGVAWILYFQSIVLGPV
                     AIVGTLSAAYPALTVVFARFFLREELAPLQYLAVTLVIGGCLGLSHTPSSAGSQSASR
                     RWIPLSLLALVCWAASNTIIKYSYSLPGANEANLAVCSSIGGALTLGVFGLLFGRRGA
                     HSLRAWVHSFVPMGMMAGGSLGLI"
BASE COUNT          401 a          817 c          805 g          504 t
ORIGIN      
        1 tgccggcgat catcgccggc tttggcgccg ggctgccgct ggtcctgctc ggagcctggc
       61 tgttcgagct gacgccggaa ggcttcaagc tgacgagcag cgtggaccgg caccgctcca
      121 tccgcgagct cacggcccgc aagctcgacg tcatcatcgc cattctgttc gcgctcgccg
      181 tcgtggcgat gctcgccgat cgctggattt tccatcgccg gccagatgaa tcggtcgtgc
      241 cgattttcat cgtggtcttt ctggtgttgc tggccgatcg ggtcatcgga cgcggcgggc
      301 cgccgggtgc cacgcgcgcc gccgccgcgc cgcttacgac aggtgcgacc gcaccgccct
      361 cgatcgcggt catgccgttc gtcgacatga gtgagcagaa ggaccaggag catttctccg
      421 atggcttatc cgaggagctc atcaccctgc tgaccaaggt ggctgatgtg cgcgttccgg
      481 cgcgcacctc ctgcttttac ttcaagggcc ggcagtgcac ggtggccgag atcgccgcgg
      541 cactgcatgt cgagcatctg ctcgaaggaa gcgtacgcaa gtccggagac aaggttcgta
      601 tcaccgtgca gctcatccgt gtcgacagcg gttaccacgt ctgggcggag acctacgacc
      661 gcaagctcga cgacatcttc gaggtccagg cgcagatcgc cactgccgta gtgcgggcgc
      721 tcaaggccac actgctgagc gcgaccctac ccgcgagact cgggctgcac agctcgcaag
      781 cctacgagct ctatctgctc ggacgcttcc attggaataa gcgctcgcct gatgagtttc
      841 gcaaagccat cgagtgcttc agacaggcga tcgtcgctca gccgaactat cccctcgcat
      901 tctgcggcat cgccgatagt tacagcctgc tgccgatcta tgacagcgcg gtgcaggcga
      961 cggagaccat gccgcaggcg aagcaggcgg cgctgaaggc gctgtccttg gacgagggtc
     1021 tggcggaagc gcatgcgtcc ctcgctctga ctcttgcgct ttttgattac gactgggcgg
     1081 ccgcggagcg gcacttgacg cgcgccatcg agctgaatcc gaactctccc tcgcctcatc
     1141 agtggtattg cgagctgctc gtgaacaccg ggcggctcga ggagggcctg gaggagggcc
     1201 ggctgtcggt gcacatcgac ccactgtcgc tggtcgccaa tctcgggctc ggcatcgcat
     1261 tgaactccgc acgtcgttat gacgaggcga tcgctcaact gcaaaagacg ctggagctcg
     1321 acgcgcggtt cgctgatgcc agctacttcc ttttcgaggc ctacgccaac caaggcaagt
     1381 acagggaagc ggtcgaggtg tacgcccggc agaagagcct cgacggcgag ccagccgcgg
     1441 aaatcacggc gatgcagcag gcgttcaccg agcactcgtg ggagggcttt ttgcggcacc
     1501 gcatcagctt tctcgaggcc cagccgcacc cgattgccga ggaggtcgct gccctttgcg
     1561 cacgggttgg tgaccaggag agggcgtgcg catggctcga gaagtcctat gaggggcgca
     1621 gcgccaggtt gacacatctt aaagtggatg cgcgctacga caaattgcgc tcgaacccgc
     1681 gcttcgcgcg gcttgttcac cgcatcggtc tgagctgacg gagccgtcgt gcattcgagc
     1741 cgctgcgcgt gatgtacatc gcggcccgcg aattcctcgc ccgcgcggca gaagccgcgc
     1801 aatgactccc ggcagcgtcg tcgttccggt ctacgtctgg ctcactcccg cgctgatcgc
     1861 cctcttcctc tatggcctcg gccaggggct ggtaaagaag tggatcggcg aggtatcacc
     1921 ggcgcggttc tgtctttact atgtgctcgc cacctcaatc gtcaatttcg gttttttcct
     1981 cacccaggac catccgccac tgctggcggc gagcggcacg ggattcctgg tcgcgggggt
     2041 gctcgcctac gtgttcgatg gagtggcgtg gatcctgtac ttccagtcga tagtgctagg
     2101 accggtggcc attgtgggca cgctgtcggc ggcctacccg gcgctgaccg tggtattcgc
     2161 gcgcttcttc ctccgcgagg agctcgctcc cttgcaatat ctggcggtca cgctcgtcat
     2221 cggcggctgc ctcggcctct cacacacgcc ttcgagcgca ggcagccaga gcgcgagccg
     2281 ccggtggatc ccgctgtctt tgctcgcgct ggtctgctgg gctgcctcca acacgatcat
     2341 caagtactcc tactctctac ccggcgcgaa cgaggccaac ctcgctgtct gcagctcgat
     2401 cggtggcgcg ctcacactcg gtgttttcgg attgctgttc gggcggcgcg gggcgcactc
     2461 cttgcgggcg tgggttcatt cgttcgtgcc gatggggatg atggccggcg gcagcctcgg
     2521 gctaatt
//
LOCUS       DATLKL010000547         2997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_51516, whole genome shotgun sequence.
ACCESSION   DATLKL010000547 DATLKL010000000
VERSION     DATLKL010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51516"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..2443
                     /locus_tag="VM713_07350"
                     /pseudo
     CDS             58..2443
                     /locus_tag="VM713_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            2454..>2997
                     /locus_tag="VM713_07355"
     CDS             2454..>2997
                     /locus_tag="VM713_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVH84114.1"
                     /translation="MIDVYYWGTPNGLKIKLFLEEAGLPYRIIPVDIGKGEQFKPEFL
                     KISPNNRIPAIVDHDPRDGGPPLSLFESGAILLYLAEKTGRFIPPAVRGRAKVLEWLF
                     WQVGGLGPMAGQNGHFNNYAPEKVPYAIDRYTKETNRLYGVLDRQLAGREYIAGDYSI
                     ADMACYPWIVPHERHRQNLDE"
BASE COUNT          506 a         1023 c          948 g          520 t
ORIGIN      
        1 agcgtcgacc tgaagaccaa caccgatgtc acgtcgctgg acgagttccg ccagctggtg
       61 atcaagcgcc agaacggcgc actggtgcgg ctccaggacg tggccaacgt ggtgctgggt
      121 gccgagaact acgacttcaa tgtggccttc agcggccagc gctcggtgtt catcggcatc
      181 aaggtcgccc cgcgcgccaa cattctcgac gtcgcggcac gggtgcgcgc tgcgctcccc
      241 gatatcgcgg cgcagctgcc gaccggagtg acgcagaagc tcgcctacga tgccaccaag
      301 ttcgtcacga gctcgatcaa ggaagtcatc aagactctgg tccaggcgct cgtcatcgtc
      361 acggtggtga tcttcctgtt cctcggcagc ccacgcgcgg tggtgatacc ggtcgtggcc
      421 atgccgctgt cgctgatcgg cgcctttttc ctgatgcagg cgctcggcta ctccatcaat
      481 ctcctcacgc tgcttgcgct ggtgctggcg atcggactcg tggtcgacga tgccatcatc
      541 gtggtcgaaa acgtcgaccg gcacctcaag gaaggggtga cgccgctcaa ggcggcgctg
      601 caggcggcgc gcgagctcgg cggacctatc attgcaatga ccatcgtgct ggtcgcggcc
      661 tacgtgccca tcggcctgca gggcgggctc acgggcgccc ttttcaccca gtttgccttc
      721 acgctcgccg gggccgtcac ggtctcggcc gtcatcgcgc actctctcgc cgatgatgtg
      781 cgcgaaggtg ttcacgagcg agcaggaaga gggccgcttc acgcacttcc tcgaccgcac
      841 cttcaaccgg ctgcgcaacg cgtacctgcg gcggatgttc aactggctca cgacctggcc
      901 cgcggtggtg ctgttcgggg cactgctgct gcccgcggta gcggcgctgt tcatgacgtc
      961 gaaggccgag ctcgcgccgg cagaggatca gggcatcgtg ctcgaggcca ccaccggtcc
     1021 gccggatgca accctcaccc agatgcaggc ctactccgac gccgaattcg cgatcgcgaa
     1081 gaagctgccg gaatatcagc agatgttcca gctgaccggc gccccgaccg tgaaccaggg
     1141 cttcggcggc gtgctgttca ccccctggga ggatcggcac cgcggcgccg gcgagctgca
     1201 gatgctcctg cagaacgagt ggaacaagat cccgggcgcg cgggtcgcgg cgttccagtt
     1261 cccggccctc cccggctcgc agggcttccc gatccagttc gtcatcacca ccaccgagcc
     1321 gatcgagaac ctgaatgagg tcgcgacgcg ggtgctggac aaggcgcgcg cgagtggcat
     1381 gttctggttc atcgacgcgg atctgaagct cgacaagccg cagcagaccg tgaatgtcga
     1441 ccgcgacatg gtgagcgatc tcggactcac gcagcagagc gtaggcggcg cgctcgccgc
     1501 ggccttgggc ggcggctacg taaattactt ctcgatcgcc ggccgctcct acaaagtcat
     1561 cccacaggtg ctgcaggtcg atcgcctcaa ccccgaccag gtgctcgatt actacgtcca
     1621 ctccggcaac ggcacactca tcccggcgcg caccattgcg agcctcaaga cctccaccgt
     1681 ccccgagacg atcagccact tccagcagct caacgccgcg accctcggcg gagtgacggg
     1741 ggcagctgag gcggacgcac tcaagttgct gcgcgacacg ctcaagcagg ttgcgccgac
     1801 cggctacaac gccgactact cgggggagtc ccggcagtac atgctgggat ccggcagctt
     1861 cgtgttcacg ctgctctttg ccatcgtgat cgtctatctg gcgctcgcgg cgcagttcga
     1921 gagcttccgc gatccggcag tgatcctggt gtccgtgccg atggcgctct tcggcgcgct
     1981 gatcttcatc aacctcgggg cgctgggcat ctcgctcaac atctacactc aggtcgggct
     2041 ggtcacgctc ctcggcctgg tcagcaagca cggcatcctg atagttcagt tcgccaacga
     2101 gctgcagcgg gccggacatg ccaagctcga ggcgatcgag gaggcggcgt cggtgcggct
     2161 gcgcccgatc ctgatgacga ccgccgcgat gtgcttcggc gtgatcccgc tcgtgatcgc
     2221 ctcgggcgcg ggcgccgcgg gccggcgcaa catgggcgtc gtgctcttca ccggtctcgc
     2281 catcggcacc ctcttcacgc tgttcgtggt gccggcgatg tacgtgttca tcggcgccga
     2341 ccacggccac gaggcgcgcg ctcgcggcca tcgccccgag gactccggcg agcagcacgg
     2401 cgattctccg ggcggcgcgc tcgagccggc gccgggccgc taggagagca gccatgatcg
     2461 acgtttacta ctggggtacg cccaacggac tgaagatcaa gttgttcctg gaagaggcgg
     2521 gactccccta ccgcatcatc ccggtcgaca tcggcaaggg cgagcagttc aaacccgagt
     2581 tcctgaagat ctcgcccaac aatcgcatcc cggcgatcgt cgaccacgac ccgcgggacg
     2641 gcggaccgcc gctttcgctg ttcgagtccg gcgccattct tttatatctg gcggaaaaga
     2701 ccgggcgctt catccccccg gccgtccgcg gccgcgccaa ggtgctcgag tggttgttct
     2761 ggcaggtcgg gggcctgggt cccatggccg ggcagaacgg ccacttcaac aactacgcac
     2821 ccgagaaggt cccctacgcc atcgaccgct acaccaagga gaccaaccgc ctctacgggg
     2881 tgctcgaccg gcagctcgca ggacgcgaat acatcgcggg cgactattcg attgccgaca
     2941 tggcgtgtta tccctggatc gtgccgcacg agcggcaccg gcagaatctc gacgagt
//
LOCUS       DATLKL010000548         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_153382, whole genome shotgun sequence.
ACCESSION   DATLKL010000548 DATLKL010000000
VERSION     DATLKL010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153382"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VM713_07360"
     CDS             complement(<1..238)
                     /locus_tag="VM713_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84115.1"
                     /translation="MTERGPKDSVSTLRDLPQGIEPGRDLWPQIEARIAGRGGAVPVP
                     GAAGAARRGAAAPRAWLAAAAMVACVAVGVWIGRS"
     gene            complement(240..803)
                     /locus_tag="VM713_07365"
     CDS             complement(240..803)
                     /locus_tag="VM713_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVH84116.1"
                     /translation="MTLAQVEDSAIDWERALVERARAGDTRAFERLYREHTGRVYGLC
                     LRMTREPHTAEDCTQETFINAWRALARFETRSSFGTWLHRIAVNVALAKRRKVARDEP
                     VADVEVEEEGVEGDWTLETPVEVQEIEAAIGTLPEGARDALVLHALYGYSHTEAAQML
                     GVAEGTCKAQLHRARKLLRERLNVEVG"
     gene            complement(1066..1926)
                     /gene="hemF"
                     /locus_tag="VM713_07370"
     CDS             complement(1066..1926)
                     /gene="hemF"
                     /locus_tag="VM713_07370"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HVH84117.1"
                     /translation="MSTPSARRRGAAHADGFAATATACFRDLQARFCAAFEALEPASR
                     FEARAWRKPDGHRLQGGGEARLMRGSVFEKVGVNVSHVWGMLAPEARPQVAGAEESGG
                     RFTACGISLVAHMMNPHVPAIHLNLRYLATSRAWFGGGSDLTPTFPVAEDTAEFHAAL
                     RAACDAFRPDAYEQYKARCDRYFYLPHRQEPRGVGGIFFDDLAAGAAGNEFAFVRGVG
                     EAVLGVYPRIVARRKDTPYDEAARERLLLKRGRYVEFNLVYDRGTRFGFSTDADPDAY
                     LMSLPPLVRW"
     gene            complement(1913..>2119)
                     /locus_tag="VM713_07375"
     CDS             complement(1913..>2119)
                     /locus_tag="VM713_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HVH84118.1"
                     /translation="LGPQKAVQGNLDPAALFAPPGELTRAAADVLAEAGSAPGHIFNL
                     GHGIWPNTDPDAVARLVDFVHEHP"
BASE COUNT          323 a          771 c          706 g          319 t
ORIGIN      
        1 cgctccgccc gatccacacg cctaccgcga cgcaggccac catggccgcc gccgcgagcc
       61 aggcgcgcgg tgcggccgcg ccgcggcgtg cggctcccgc cgcaccgggc acgggcacgg
      121 cgccgccgcg tccggcgatc cgcgcttcga tctgcggcca caggtcccgc ccaggctcga
      181 tgccctgcgg caggtcgcgc agcgtactca cgctgtcctt cgggccccgc tctgtcatgt
      241 cagccgacct ccacgttcaa gcgctcgcgc aggagcttgc gggcgcggtg cagttgcgcc
      301 ttgcacgtgc cctctgcaac gccgagcatc tgcgccgcct cggtgtgcga gtaaccgtac
      361 agggcatgca gcaccagcgc atcccgagca ccctcgggca acgtcccgat cgcggcctcg
      421 atctcctgca cttccaccgg cgtctccagg gtccaatcgc cctcgacgcc ctcctcctcg
      481 acttccacgt ccgccaccgg ctcgtcgcgg gcaaccttgc ggcgcttggc gagcgccacg
      541 ttgactgcga tgcggtgcag ccaggttccg aacgagctgc gcgtctcgaa ccgggcgagc
      601 gcgcgccagg cgttgatgaa ggtctcctgg gtgcagtcct ccgccgtgtg cggctcgcga
      661 gtcatgcgca ggcacaggcc atacacccga ccggtgtgct cgcggtagag ccgctcgaac
      721 gcgcgggtat ccccggcgcg ggcccgctct accagcgccc gctcccagtc gattgctgag
      781 tcctcgacct gcgcgagtgt catcggtgaa ttagaggcgg cacggttcat cgcggtttaa
      841 aggtccgtcg ctgaaagact acaggttgcg gaactcggcc aacctcatgt cctctaacgg
      901 cactcaacgg aaaggaggac tctgtggcaa cgcgacaaaa gtgggctctc gactcgattg
      961 ccgcggcgct gggcgagcac tggcgcaagg caagcgagag cactgtgtcg ctcttctggc
     1021 cgactccggt caagtggctg gaagcgcggc gcctgcgtag acgccttacc accgcaccag
     1081 cggcggcaga ctcatcaggt acgcgtccgg atccgcatcg gtgctgaagc caaagcgggt
     1141 gccccggtcg tagacgaggt tgaactcgac gtagcgcccc ctcttcagca gcagccgctc
     1201 gcgcgccgcc tcgtcgtaag gcgtgtcctt gcgccgggcg acgatccgcg gatagacgcc
     1261 gagcactgct tccccgacgc cccggacaaa ggcgaactcg ttcccggccg cgcccgcggc
     1321 caggtcatcg aagaagattc cgccgacgcc gcgcggctcc tgccggtgcg gcaggtagaa
     1381 gtaccggtcg caccgggcct tgtactgctc ataggcgtcg ggcctgaacg cgtcgcacgc
     1441 cgcacgcagt gcagcgtgaa actccgcagt gtcttcggca accgggaacg tcggcgtgag
     1501 gtcagagccg ccgccgaacc aggcgcggct ggtggcgaga tagcgcaggt tgaggtgtat
     1561 cgccggcacg tgggggttca tcatgtgggc gaccagcgag atgccgcagg cggtgaagcg
     1621 gccgccggac tcctcggctc ctgccacctg cgggcgtgcc tcgggggcca gcattcccca
     1681 cacgtggctc acgttgaccc ccaccttctc aaagaccgac ccgcgcatga ggcgcgcctc
     1741 gccaccgccc tgcagacggt ggccgtccgg cttccgccag gcgcgcgcct cgaagcggct
     1801 cgccggttcg agagcctcga acgctgcgca gaagcgcgcc tgcagatcgc ggaagcaggc
     1861 agtcgcggtc gccgcgaaac catccgcatg cgccgcgccc cggcggcggg cgctaggggt
     1921 gctcatgcac gaagtccacg aggcgtgcca ccgcatccgg atcggtgttc ggccagatgc
     1981 cgtgcccgag attgaatatg tgtcccgggg cgctgcccgc ctccgccagc acgtccgcgg
     2041 cagcacgcgt gagctcgcca gggggcgcga agagcgccgc ggggtcgaga ttgccctgca
     2101 ccgctttctg tggtcccag
//
LOCUS       DATLKL010000549         3650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_142014, whole genome shotgun sequence.
ACCESSION   DATLKL010000549 DATLKL010000000
VERSION     DATLKL010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142014"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /gene="cysD"
                     /locus_tag="VM713_07380"
     CDS             complement(<1..736)
                     /gene="cysD"
                     /locus_tag="VM713_07380"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004694369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HVH84119.1"
                     /translation="MSRAESLTHLDRLEAESIHVFREVIAECDKPVMLYSIGKDSAVM
                     LHLAVKAFYPATPPFPLLHVDTTWKFRAMYEFRDAVARRTGMEPIVYVNQEGLARGIN
                     PFTHGSQVHTDVMKTQALRQALDAHGFDAAFGGARRDEEMSRAKERIFSFRSAQHRWD
                     PRQQRPELWSLYNARHRPGESMRVFPLSNWTELDVWQYIWRENIAIVPLYLAAPRPVV
                     ERDGALIMVDDERLPLKPGEQPTLKKV"
     gene            918..1961
                     /locus_tag="VM713_07385"
     CDS             918..1961
                     /locus_tag="VM713_07385"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HVH84120.1"
                     /translation="MRLALSSGRVWGLLLSLPLAGGLAQTPAPPETPAAAVPSPAPGA
                     ASPVPTPAPPAASPSTGAPPLSEVVVEAPEPRFVAKTTRDRIGRIWAPVLIDGKGPFR
                     LVLDTGANHSAVIPSTAKALGLPNAPEATVVTGFTGSAVVPAISVSRMEVGDLLMGPT
                     TLPIVPDVFGGAQGVLGNEGLAGKRIVADFSHDRLEISGSHADPARIGFSVVRLQVVE
                     GGLLIADVKVGGIRCKAIIDTGAQGSVGNLPLREALMRHPPRDAKHAEVVGVTLDVQN
                     GDSVPAPQIEMGGLTITGVHITFGDMYLFQHWHMTKRPVLLLGMDLLGSFDVLIMDYR
                     MHELQIRLTRGSV"
     gene            complement(1973..2461)
                     /locus_tag="VM713_07390"
     CDS             complement(1973..2461)
                     /locus_tag="VM713_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84121.1"
                     /translation="MTPNPWLEGLRDASAALQSVLGALLEAERRFAPFASPLERLKHI
                     TTDPAWAWLQPLYGLIADVDHALAVARDLPESEAAAIGAHARELLTGGGGPAEMQFLE
                     HYRGMLQTDPATAMAHAGALRALQALPPEAEDQAERLHARHQWNERRRFLRMGPHGRG
                     TS"
     gene            complement(2458..3141)
                     /locus_tag="VM713_07395"
     CDS             complement(2458..3141)
                     /locus_tag="VM713_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVH84122.1"
                     /translation="MKQVADSRTVFLFDVDNTLLDNDAVQADLSAHLERKFGRASRDR
                     YWSLFEELRGELGYADYLGALQRYRLENLDDPQLLQVSSFLVDYPFAERLYQGALAAL
                     EHCGRLGQTVILSDGDVVFQPRKVQRAGLWDAVSGRVLIYLHKEQMLAAVERRYPAEH
                     YVMIDDKLRILTAMKRVWGARLTTIFARQGHYALDPRELASYPAADLAVQRIGEIAGC
                     DPGWLKGMR"
     gene            3267..>3650
                     /locus_tag="VM713_07400"
     CDS             3267..>3650
                     /locus_tag="VM713_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HVH84123.1"
                     /translation="MKSQFFASCAAQCRPRLVAGAAAVLLVLAGCGYDDNYCGGCFIG
                     PQPTVNVPNSIAIADVNGDGVADLLVATTADSGGFANPGFANVILGNKASPGTFDAGV
                     QYAATGTNLSGIAVTDLTGSGHLDLV"
BASE COUNT          574 a         1263 c         1201 g          612 t
ORIGIN      
        1 gaaccttctt cagggtcggc tgctcgccag gcttcagcgg caaccgctcg tcgtccacca
       61 tgatgagagc gccgtcgcgc tcgaccaccg gacgcggagc ggccaggtac agcggcacga
      121 tggcgatgtt ctcgcgccag atgtactgcc agacatcgag ctcggtccag ttcgagagcg
      181 gaaacacccg catgctctcg cccggacgat ggcgcgcgtt gtagagcgac cagagctcgg
      241 ggcgctgctg gcgtggatcc cagcggtgct gcgccgagcg gaacgagaag atgcgctcct
      301 tggcgcggct catctcctcg tcgcggcgcg cgccgccgaa ggcggcgtcg aagccgtgag
      361 cgtcgagcgc ctggcgcagc gcctgcgtct tcatcacgtc ggtgtgcacc tgcgagccat
      421 gggtgaacgg gttgatcccg cgcgcgagcc cctcctggtt cacgtacacg atcggctcca
      481 tacccgtgcg ccgggcgacc gcgtcgcgaa actcgtacat cgcacggaat ttccaggtcg
      541 tatcgacgtg cagcagcgga aacggcgggg tcgccgggta gaaggccttg accgccaggt
      601 gcagcatcac cgcgctgtcc ttgccgatgg aatagagcat cacgggcttg tcgcactcgg
      661 cgatcacttc cctgaagacg tgaatgctct cggcttcgag ccggtcgagg tgggtgagag
      721 actcggctcg actcatcggg cgtgactcat ctggctcggc tcatctggct gaagctcctg
      781 cgggtgcgcg gctcgcatgg cagagtatcg cccgcccccc ggacgcggca cggtagcgga
      841 ttgtaatagg cttcggacgg ggccgattcg ctgtattctg cgaccgtgat cactcacgcg
      901 aggctgtctt gaacgcaatg cgcctggcgc tatcttcggg ccgcgtgtgg gggctgctcc
      961 tgtcgcttcc cctggcgggc ggccttgccc agaccccggc accacccgag accccggcgg
     1021 cggcggtgcc gtcaccggct cccggcgccg catcccctgt gccgacgccg gcgccgcccg
     1081 cagcgagccc ctcgaccggc gcgccaccgc tttccgaggt cgtggtcgag gcgccggagc
     1141 cgcggttcgt cgcgaagacc acccgtgacc gtattggccg tatctgggcg ccagtgctga
     1201 tcgacggcaa agggcccttc cgcctcgtgc tcgataccgg cgcaaatcac tcggccgtca
     1261 tcccttccac tgccaaagcg ctcggattac cgaacgcccc cgaggccacc gtggtcacgg
     1321 gattcacggg ctccgcggtg gtcccggcga tcagcgtttc gcgcatggag gtcggtgacc
     1381 tgctcatggg cccgaccacg ctgccgatcg tgccggacgt gtttgggggc gcacagggag
     1441 tgctcggtaa cgaggggctg gcgggcaagc gtatcgtcgc ggacttcagt cacgaccgcc
     1501 tggagatctc tggctcccac gccgaccccg cgcgcatcgg cttcagcgtc gtgcgcctcc
     1561 aggtggtcga gggcgggctg ctgatcgctg acgtcaaggt cggcggcatt cgctgcaagg
     1621 ccatcatcga caccggcgcg cagggcagcg tcggcaatct gccgctgcgg gaggcactca
     1681 tgcgtcatcc gccgcgtgat gccaagcacg ccgaggtcgt cggcgtgacg ctcgacgtgc
     1741 agaacggcga cagcgtgccc gcgccgcaga tcgagatggg cggcctcacc atcacgggcg
     1801 tccatatcac cttcggcgac atgtacctgt tccagcactg gcacatgacg aagaggcccg
     1861 tcttgctgct cggcatggat ctgctcggct ccttcgacgt gctgatcatg gactaccgta
     1921 tgcatgaatt gcagatccgg ctgacccgcg gatcggtgtg aggcgcggcg cgtcaggagg
     1981 taccccgccc gtgcggtccc atgcgcagga agcgccggcg ctcgttccac tggtgccgcg
     2041 catgcagccg ctcggcctgg tcctccgctt cgggcggcag cgcctggagc gcacgcaacg
     2101 cgcccgcgtg cgccatcgcg gtcgccggat cggtctgcag cattccgcgg taatgctcga
     2161 ggaattgcat ctcggcaggt ccgccgccgc ccgtcagcaa ctcgcgcgcg tgcgcgccga
     2221 tcgccgcggc ctcgctctcc ggcaggtcgc gcgccactgc gagcgcgtga tcgacgtcgg
     2281 caatcaggcc atagagcggc tgcagccagg cccaggccgg atcggtggtg atgtgcttca
     2341 gccgctcgag cggtgaggcg aagggcgcga agcgccgctc cgcctcgagc agcgcgccga
     2401 gcacgctctg cagcgccgcg ctcgcgtccc tgagtccctc aagccacggg ttcggcgtca
     2461 tctcatgccc ttcagccagc ccgggtcgca tccggcaatc tcgccgatgc gctgcacggc
     2521 gaggtccgcg gccggatagc tcgccagctc gcgcgggtcg agcgcatagt gcccctggcg
     2581 agcaaaaatc gtcgtcagcc tcgctcccca gacacgcttc atcgccgtca gtatgcgcaa
     2641 cttgtcatcg atcatcacgt agtgctcggc gggatagcgc cgctcgaccg ccgcgagcat
     2701 ctgctcctta tgaaggtata tgagcacccg gcccgacacg gcgtcccaca gccccgcgcg
     2761 ctgcaccttg cgcggctgaa acacgacgtc cccgtccgac aggatgacgg tctgcccgag
     2821 acggccgcag tgttcgagcg cggccagcgc cccctgatac aggcgctcgg caaacggata
     2881 gtcgaccaga aacgaggaca cctgcagcag ctgcggatca tcgaggttct ccagccggta
     2941 gcgctgcaga gcgccgagat agtccgcata gccgagctcc ccccgcagct cctcgaacaa
     3001 cgaccagtag cgatcgcggc tggcgcggcc gaacttacgc tccaggtgcg cgctcagatc
     3061 ggcctgcacg gcatcgttgt cgagcagcgt gttatccacg tcgaacagaa agacagttct
     3121 cgaatctgcc acttgcttca cgttctcacg gggcgtagtg cgggcagtgt acgctgccgg
     3181 ggaacagttc gaggctgcgg gggtcaaccg ggtgcgactg ggcgcgttaa gctcgtttag
     3241 gcgtattttg cacggaggga gcgcagatga aatctcaatt ttttgcgagc tgcgccgcgc
     3301 agtgccgccc gcggctcgtc gccggcgccg cggcagtgct gctcgtcctg gcgggctgcg
     3361 gctacgacga caactactgt ggcggatgct tcatcggccc acagccgacg gtcaacgttc
     3421 ccaactccat cgcgattgcc gatgtcaacg gtgacggcgt agcggatctc ctggtcgcca
     3481 cgaccgcaga ctcaggaggc ttcgccaacc ccggcttcgc caatgtcatc ctcggcaaca
     3541 aggcctcgcc gggaaccttc gatgccggcg tgcagtacgc ggccaccggg accaacctct
     3601 cgggtatcgc cgtcacggac ctcacgggct cgggccatct cgacctcgtc
//
LOCUS       DATLKL010000550         1680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_17668, whole genome shotgun sequence.
ACCESSION   DATLKL010000550 DATLKL010000000
VERSION     DATLKL010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17668"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..579)
                     /locus_tag="VM713_07405"
     CDS             complement(97..579)
                     /locus_tag="VM713_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HVH84124.1"
                     /translation="MTHNYRGSVEQAIALLNHAVATEIVCVLRYKYHAVAAAGISSQA
                     VREEFAQHAKEEEQHLDLLCERINQLGGKPRLNPAQVTGRAASQYVEGETLIDMIREN
                     LIAERIAIETYRDMVRYFADKDPSTRVLLERILAQEEEHANDMHDLLVQHEGRPTLRR
                     "
     gene            complement(986..1417)
                     /locus_tag="VM713_07410"
     CDS             complement(986..1417)
                     /locus_tag="VM713_07410"
                     /inference="COORDINATES: protein motif:HMM:NF016834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lecithin retinol acyltransferase family protein"
                     /protein_id="HVH84125.1"
                     /translation="MSREPPIGAHVLTPRSGYIHHGIFVGRGRVVHYRGLSRGFSPGP
                     VEEVTLTQFAQGRPVWIAPRRESAASPEEVVRRARLRIGENRYRIFTNNCEHFCEWCL
                     SGDPRSYQVEALVDCCRAWLERLTSLVRRAGRSELSTDTFA"
BASE COUNT          289 a          590 c          487 g          314 t
ORIGIN      
        1 acccggcgct tgcatcgtcc acaccatcgg ccattccacc cgcacgatcg acgagctcgc
       61 agggttgctc gcgcgagcct tcaggagcca ctcggcctag cgtcgcagcg tggggcgtcc
      121 ttcatgctgg accaggagat cgtgcatgtc gttggcatgc tcttcctcct gcgccaagat
      181 gcgctcgagc aggacacgcg tgcttggatc cttgtccgcg aagtagcgca ccatgtcgcg
      241 gtaggtctcg atggcgatgc gctcggcgat cagattttcc ctgatcatgt cgatgagcgt
      301 ctcgccctcc acgtattgcg aggcggcgcg acccgtcacc tgcgctggat tcagacgggg
      361 cttgccgccc agctgattga tcctttcgca aaggagatcg aggtgctgct cctcttcctt
      421 cgcgtgctga gcaaattcct cgcggacagc ttggctcgat attccagcgg ctgccacggc
      481 gtggtacttg tagcgcagga cgcacacaat ctcggtggcg accgcatgat tgagaagcgc
      541 aatcgcctgc tcaacgctgc cccgatagtt gtgcgtcacc gcccctgaac tcagatcctc
      601 tcgtgccgca agtcgtatcg cagccagact ctcgctgaac ctgcgatctt gcctttcaga
      661 gatgagctca ctcaggtccg gggtcgcgtg ccgccgcccg gctacctcgg cggccttgag
      721 ctcgcgcgcc tcgcgcgcat tacgaggcgc cgcctcgcgc gcggcacgct cgacgtctgc
      781 ccgccgcagg taacgcttga ccgaccgccc gaacctccgc gccgcctggt aatctccctc
      841 cccctgcagc cggacggctg agcgggcacc tacagagccc ccactccgcc gcctgcgctc
      901 cgtcgtattc gacgtcctca tcgctcgcct cccgcaggcg atctcgcccc agggccaata
      961 gaccagaagt gcgcgcgcga atcgattagg caaaggtatc ggtcgatagc tccgaacggc
     1021 cggcgcgtct taccagcgag gtgagcctct ccagccaggc ccggcagcag tccaccaaag
     1081 cctcgacctg atagctgcga gggtcgccgc tgaggcacca ctcacagaaa tgctcgcagt
     1141 tgttcgtgaa gattcggtag cgattttccc caattcgcag gcgcgcgcgc cgtaccactt
     1201 cctccgggct ggcggcgctc tcgcgccgcg gcgcgatcca gacgggacgc ccttgagcga
     1261 attgcgtcaa tgtcacctcc tcgaccggac ccgggctaaa gccgcgggac aggccacggt
     1321 agtgcaccac ccgcccgcga cccacaaaga tcccatgatg aatataacca gagcgaggcg
     1381 tgagcacgtg cgccccgatc ggcggctcgc ggctcaactc cgccgctaga tccagagctc
     1441 tccacacgtg ctcccgcgtg cggcgcttga gttcccggcg ccggtcgtcc tcgcctgaca
     1501 tctggaatgt gtccgactct gctggcacgc ggacatcaca tcgctttccg ggccgcgcgt
     1561 cgattcatcc aaagtgtcta tcgatacgaa cggatagctg cacgctgagg cagcgtcgcc
     1621 aagtttcccg accagagcac cggaatactg ttttccctct aatggaaaga tgtgatggcg
//
LOCUS       DATLKL010000551         2943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_164461, whole genome shotgun sequence.
ACCESSION   DATLKL010000551 DATLKL010000000
VERSION     DATLKL010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164461"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VM713_07415"
     CDS             complement(<1..567)
                     /locus_tag="VM713_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH84126.1"
                     /translation="MIDQFVEFCLRRRIAVIVIAVLLSVFGVYAWETLSVEAYPELGD
                     VSAQVTTQMPGLAAEEVEQLITVPLEREINGTPGLLLMRSESTFGLSLITVLFRDGYE
                     DYWVRQRLLERINQVTLPAGAAASLDPVSGPGGEICRYTLESDTRNLQELSEIQRWTV
                     IPALKSVPGVVDDTNFGSFTTEYQLDLDP"
     gene            complement(560..1777)
                     /locus_tag="VM713_07420"
     CDS             complement(560..1777)
                     /locus_tag="VM713_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVH84127.1"
                     /translation="METHDQPRGENERSAGANEYAHGHDHAPNPRGRAFFWGGLGGGL
                     LLIVLLLAQGLWRGRGAQEEAPALIHQGVRILVPEKSPLRQRLTVAPAPAEERSTIVT
                     APGIIESDPARTVNVLPPGTGRVREVKVALGDRVQRGQVLATIDSPDLAQAYSDYDKA
                     GAAALLAAKNLKYQEEQFRIGAAAQRDLESARNDNSQAAAEYQRARVRLRAMGAAEDA
                     QGAGRLLLVRAPAPGSITAFAIAPGATINDDTQAVMTLADLSVVWVTALIAERDVGGV
                     TRDQDAEITVDAYPGKTLHGKVLFVADVIEPDSRRDKTRIALPNADASLKPNMFATVT
                     LHGPARVRVVLPSSALLMNNDRTSVFVAIAPWTFERRTVQPLLEEGTQVAIESGVQPG
                     EQVVVKGGILLND"
     gene            2135..>2943
                     /locus_tag="VM713_07425"
     CDS             2135..>2943
                     /locus_tag="VM713_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84128.1"
                     /translation="MKAGRSLWVAALLAASLLGAVLAACESAQFGNREPASGPGPGGK
                     PMRTATDAVIGLPAVAQAPVYPLTARVGVSDNFFGTHVEDPYRWLEDLSSPAVVQWVA
                     AQNAVSQPLLEELPQRAWLKKRLAELWNYERYELPVKRGAHYFFLHNDGRQNQSVLYV
                     SEQLDSQGRVLFDPNTVRADATVALSEFTPDEQGSVVAYATSDGGTDWQIWRFRRVSD
                     ATDLPDELRYTKFWGVSWAHDGPGVYYSRYPTLASGRGDDGGRPALYFHALG"
BASE COUNT          423 a         1038 c         1003 g          479 t
ORIGIN      
        1 tgggtcgagg tcgagctggt actcggtggt gaagctaccg aagttggtgt catcgaccac
       61 gcccggcacg gacttgagcg ccggaatgac cgtccagcgc tggatctcgg agagctcctg
      121 aaggttgcgg gtgtcggact cgagcgtata gcggcagatc tcgccaccgg gaccgctcac
      181 cgggtcgagc gacgcggcag cgcccgcggg cagcgtcacc tggttgatgc gctcgagcag
      241 ccgctgccgc acccagtagt cctcgtagcc gtcgcggaac agcaccgtga tcagcgacag
      301 gccgaaggtg ctctccgagc gcatgagcag cagccccggc gtgccgttga tctcgcgctc
      361 gagcggcacc gtgatgagct gctcgacctc ttcggcggcg aggcccggca tctgcgtggt
      421 gacctgcgcg gagacatcgc cgagctccgg gtaggcctcg acgctcaagg tctcccaggc
      481 atagacgccg aagacgctca agagcaccgc gatcacgatg accgcgatgc ggcgtcgcag
      541 gcagaactcg acgaactggt caatcattga gcaggattcc gcccttgacc acgacctgct
      601 cgcccggctg gacgcccgat tcgatcgcga cctgcgtgcc ctcctcgagc agcggctgca
      661 cggtgcggcg ctcgaaggtc cagggggcga tcgcgacgaa tacgctggta cggtcgttgt
      721 tcatgagcag cgccgagctc ggcagcacca cccgtacgcg cgccgggccg tgcagcgtca
      781 ccgtcgcgaa catgttgggc ttgagggaag cgtcggcatt cggcaaggcg atgcgggtct
      841 tgtcgcggcg cgagtccggc tcgatcacgt ccgcgacgaa cagcaccttc ccgtgcagcg
      901 tctttcccgg gtaggcgtcc acggtgatct ccgcatcctg atcgcgtgtc acgccgccga
      961 cgtcgcgctc ggcgatgagc gccgtcaccc acaccaccga cagatccgcg agcgtcatga
     1021 ccgcctgggt gtcgtcgttg atcgtcgcgc cgggggcgat cgcgaaggcg gtgatgctcc
     1081 cgggcgcggg ggcgcgcacc aggaggagcc gcccggcacc ctgcgcgtcc tccgcagcgc
     1141 ccatggcgcg cagccgcacg cgcgcgcgct ggtactcggc cgccgcttgg ctgttgtcat
     1201 tgcgtgccga ctcgaggtcg cgctgcgcgg cggccccgat gcggaactgc tcttcctggt
     1261 acttgaggtt cttcgccgca agcagcgccg cggcgccggc cttgtcgtaa tcggagtacg
     1321 cctgcgcgag gtccggcgag tcgatggtcg ccagcacctg gccgcgctgc acccggtcgc
     1381 cgagcgccac cttgacttcg cggacgcgcc ccgtcccggg gggcagcacg ttcaccgtac
     1441 gcgccgggtc cgactcgatg atcccgggcg ccgtaacgat cgtgctgcgc tcctcggccg
     1501 gcgccggcgc caccgtgagc cgctgccgca gcggtgattt ctccggaacc aggatccgca
     1561 cgccctggtg aatcagcgcg ggggcctcct cctgcgcccc ccgtccgcgc catagcccct
     1621 gggcgagcag cagcacgatg agcaacagcc cgccaccgag gcctccccag aagaaggcgc
     1681 ggccgcgcgg gttcggcgcg tggtcgtggc cgtgcgcgta ctcgttcgcg cccgccgacc
     1741 gctcgttctc accccgcggc tggtcgtggg tttccatggc gacaggctct cggacagggt
     1801 tatcggttta tatgacttat atgtgacaga ggcccgcgca cactccccag tcggagcggg
     1861 cggccggccc ccgcggcgcg ggcgcgattg ttgggcggcg cctgcccaaa gtcaaccgct
     1921 atttaccata cccccccacc ctatctgatt gctcgggatc ggcacattgc gctgcgctgt
     1981 cctgctgcgc gcaagccctg ggcgcacgtc cctgtgcgcc cgctcggcgc tttgcgcctc
     2041 gtccctgtcg cgcccctcgc gcggcgagca aaaagcgtgg tttttgctcg ccgctcgcca
     2101 cctgcggcgg cgggcacatc cctgtgcctc cagcatgaaa gccgggcgct ccctttgggt
     2161 cgcggcgctt ctcgccgcct cgctccttgg cgcggtgctc gcggcctgcg agagcgcgca
     2221 gttcggcaat cgtgagccgg cctcgggccc gggtcccggc ggtaagccga tgcgcactgc
     2281 aaccgatgcg gtcataggcc tgccggcggt cgcgcaagcg ccggtctatc ccctgaccgc
     2341 ccgcgtcggc gtcagcgaca acttcttcgg cacgcacgtc gaggatccct accggtggct
     2401 cgaggacttg agctcgccgg cggtggtgca gtgggtggcg gcgcagaacg ccgtgtcgca
     2461 gcccttgctg gaggaacttc cgcagcgcgc gtggctgaag aagcgcctcg cggagctctg
     2521 gaactacgag cgctacgagc tgccggtcaa gcgcggcgca cattatttct tcctgcacaa
     2581 cgacggcagg cagaaccaga gcgtgctcta cgtctccgag cagctcgact cgcaggggcg
     2641 cgtactgttc gatcccaaca cggtgcgcgc cgatgccacg gttgcactct cggagttcac
     2701 gccggacgag cagggaagcg tggttgccta tgcgacctcg gacgggggca cggactggca
     2761 gatctggcgt ttccggcgcg tgagcgatgc gacggatctc cccgatgagc tgcgctacac
     2821 caagttctgg ggggtgtcct gggcgcacga cggcccgggc gtctactaca gccgataccc
     2881 aacgctcgcc agcggccgcg gcgacgatgg cgggcggccc gcgctctact tccacgcgct
     2941 cgg
//
LOCUS       DATLKL010000552         2093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_119417, whole genome shotgun sequence.
ACCESSION   DATLKL010000552 DATLKL010000000
VERSION     DATLKL010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119417"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VM713_07430"
     CDS             <1..641
                     /locus_tag="VM713_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84129.1"
                     /translation="RCTGNAGQSFGVWNAGGLNLYLEGDANDYVGKGMAAGRIVLRHP
                     RAARYSPRETVLMGNTCLYGATGGELYAAGVAGERFAVRNSGALAVIEGAGDHCCEYM
                     TGGAVCVLGRTGVNFGAGFTGGFAYVLDLERNFVDRYNHELIDISRIHPEAMQSHVQH
                     LETMLERHVAETGSLWATEIVNDLRTYLPKFWVVKPKAASIDSLIENLRRAA"
     gene            663..2081
                     /locus_tag="VM713_07435"
     CDS             663..2081
                     /locus_tag="VM713_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH84130.1"
                     /translation="MADKNLQFLDIPRADPAKSPVEARVRHFREIYAPYDAAAAASQA
                     GRCLACGNPFCEWKCPVHNYIPNWLKLIEEGNLFEAAELSHKTNSLPEVCGRICPQDR
                     LCEGACTLNDGLGSVTIGAIEKYITDEALQRGWKPDMTNVRATSRRVAIIGAGPAGLG
                     CADILVRNGVQPVVFDRHPRIGGLLTFGIPPFKLEKEVVEKRREIMEGMGVEFRLNCE
                     VGTDVPFTALLEEYDAVFLGMGTYTPVKGGFSGEELPGVHEALPYLISNIRHELGFEG
                     AERSLISMAGKRVVVLGGGDTAMDCNRTALRQGAESVTCTYRRDEANMPGSRRDYKNS
                     REEGVEFLFNRQPIEILGAERVEGVKLVETALGAPDARGRRVPQPIPGSERIVPADAV
                     VIAFGFLPSPPGWLADHGIRVHDNGRVRVSAGAANAFQTTHPRVFAGGDMVRGSDLVV
                     TAVFEGREAARGILNALEIDSR"
BASE COUNT          355 a          706 c          711 g          321 t
ORIGIN      
        1 tgcgctgtac gggcaacgcc ggccagagct tcggcgtgtg gaacgcaggg ggacttaacc
       61 tctatctcga gggcgatgcc aacgactacg tcggcaaggg tatggccgcc ggacgcatcg
      121 tgctgcggca tccgcgcgcc gcgcgctact ctcccaggga gaccgtcctc atgggcaata
      181 cctgcctcta cggcgcgacc ggtggggagc tctatgccgc cggcgtcgcc ggcgagcgct
      241 tcgcggtgcg caactccggc gcgctcgcgg tcatcgaggg ggcgggcgac cactgctgcg
      301 agtacatgac cgggggcgcg gtgtgcgtgc tggggcgcac cggcgtcaat ttcggcgcgg
      361 gcttcaccgg cgggttcgcc tacgtgctcg acctcgaacg caacttcgtc gaccgctaca
      421 accacgagct catcgacatc agccgcattc acccggaagc catgcagtcg cacgtgcaac
      481 acctcgagac catgctcgag cggcacgtcg ccgagaccgg cagtctctgg gccacggaga
      541 tcgtgaacga cctgcgcacc tatctgccga agttctgggt ggtgaagccc aaggcggcat
      601 cgatcgactc gctcatcgag aacctgcgac gcgcggcctg aaggcgcgca gcacgagcgc
      661 ccatggcgga caagaacctg cagttcctcg acatcccgcg ggccgatccg gccaagagcc
      721 cggtggaagc gcgcgtccgg cacttccgcg agatctatgc gccctacgat gccgcagccg
      781 ccgccagcca ggccgggcgc tgcctcgcct gcggcaaccc cttctgcgaa tggaaatgcc
      841 cggtccacaa ctacattccc aactggctga agctcatcga ggagggcaat cttttcgagg
      901 cggccgagct ctcccacaag accaactcgc tccccgaggt gtgcggccgc atctgtccgc
      961 aggaccgcct gtgcgagggc gcctgcacgc tgaacgacgg cttaggtagc gtcaccatcg
     1021 gcgcgatcga gaagtacatc accgatgagg cgctgcagcg cggctggaag cccgacatga
     1081 ccaacgtgcg cgccacctcg aggagggtcg cgatcatcgg cgccggaccc gcggggctcg
     1141 gctgcgccga catcctggta cgcaacggcg tgcagccggt ggtcttcgat cgccacccgc
     1201 gcatcggggg actgctcacc ttcggcattc cgccgttcaa gctggagaag gaggtggtgg
     1261 agaagcgccg cgagatcatg gagggcatgg gggtcgagtt ccgtctgaac tgcgaggtcg
     1321 gcaccgatgt cccgttcacg gcgctgcttg aggagtacga cgcggtgttc ctcggcatgg
     1381 gcacctacac gccggtgaag ggcggcttct ccggcgagga gctgcccggc gtgcacgagg
     1441 cgctgcccta tctcatttcc aacatccgcc acgagctcgg attcgaaggc gccgagcgct
     1501 cgctcatcag catggcgggc aagcgcgtcg tggtgctcgg aggcggcgac accgccatgg
     1561 attgcaaccg cactgcgctg cgccaggggg cggaatcggt cacctgcacc taccggcgcg
     1621 acgaagccaa catgcccggc tcgcgccgtg actacaagaa cagccgcgaa gagggcgtcg
     1681 agttcctctt caaccgccag ccgatcgaga tcctgggtgc cgagcgcgtc gagggcgtga
     1741 agctcgtcga gaccgcgctc ggggcccctg atgcgcgcgg gcggcgcgtg ccgcagccga
     1801 tcccagggag cgagcggatc gtgcccgcgg atgcggtcgt catcgccttt ggcttcctgc
     1861 cgagtccgcc cggctggctc gccgatcacg gcatccgtgt gcacgacaac ggccgcgtgc
     1921 gcgtctcggc gggagcggcc aacgcctttc agaccacgca cccgcgagtg tttgccggtg
     1981 gcgacatggt gcgcggctcg gacctcgtgg tgaccgcggt gttcgagggg cgcgaggcgg
     2041 cgcgcgggat tctcaatgct ctcgaaatag atagccgctg ataaaaaaag ccc
//
LOCUS       DATLKL010000553         1722 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_29167, whole genome shotgun sequence.
ACCESSION   DATLKL010000553 DATLKL010000000
VERSION     DATLKL010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29167"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VM713_07440"
     CDS             complement(<1..439)
                     /locus_tag="VM713_07440"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HVH84131.1"
                     /translation="MTRETVTLEELRQRVTDIDRQLIALVAERKAVSEEVARVKRATG
                     RSTRDYEREREVILGVRAMAEERGVSPALAEQLLRLLIRSSLTTQEQASVAAHGAGSG
                     RRALVIGGAGKMGGWFTSFLASQGFAVEVADPALPQGVSGVADW"
     gene            complement(533..>1722)
                     /locus_tag="VM713_07445"
     CDS             complement(533..>1722)
                     /locus_tag="VM713_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84132.1"
                     /translation="YNEHLDAVLHDPSPQALDSLTFKDPHALEFQGEAQFVQPLTGSL
                     WEDVPPHRAQIEALTAQNGELYQRYLALERLPGYFESARPSYLAPAIFVPTEVRYLFL
                     EDTVLRLRSGSARAQRAALDELVADVGLWRRVLTGQGLVISKMLALAYLESDYLVLAD
                     MIADPHAAVPVGPDDADTAAPLFALRDFDLGAAFAAEFRVQASLLEQTQYLYTIGWTP
                     QAARSGAPRSWTDRLGSEVSGHFFKLNATVNLFAQQAQRLMDLAPGPGVVAADRKLAD
                     SSRSAWSLIGAYNPIGKLLAGIAAPAGVTYPLRAWDAAALQRLVRAGYEIRRQRIAPA
                     DVPAFLTQHPEWSTHPGDGRPFVWRPESAELGVQTIGEQPQGRRFSIRIWQAPPAGGE
                     ATG"
BASE COUNT          257 a          611 c          607 g          247 t
ORIGIN      
        1 gccagtcggc gacgccgctc acgccctgcg gcagcgccgg atccgccacc tccactgcaa
       61 atccctggga agcgaggaag ctcgtgaacc agccgcccat cttgccggca ccgccgatga
      121 cgagcgcgcg ccgcccgctg ccggccccgt gcgccgcgac gctcgcctgc tcctgggtcg
      181 tgagcgatga gcggatcagc agccgcagca gctgctcggc gagcgccggc gatacgccgc
      241 gttcctcggc catggcgcgc acgccgagga tcacttcacg ctcgcgctcg taatcccggg
      301 tcgagcgccc ggtggcgcgc ttcacccgcg cgacctcctc gctcacggcc ttgcgctccg
      361 ccaccagtgc gatcagctgg cggtcgatat cggtcactcg ctggcgcagc tcctccagcg
      421 tcacggtctc gcgcgtcatg gaaagggtgc tctctggcgt aaatccgcct cctttctatc
      481 gaggcggggc gggcggtgca agggaagcgc ggaaatctgc cgcagcctta agtcaacccg
      541 tggcctcgcc cccggccggc ggcgcctgcc agatgcggat ggagaagcgc cggccctgcg
      601 gctgctcgcc gatggtctgg acgccgagct cggcgctctc cgggcgccac acgaacggcc
      661 gcccgtcacc cggatgcgtc gaccactcgg gatgctgtgt gagaaaggcc ggaacgtcgg
      721 ccggggcgat tcgctggcgg cggatctcgt aaccggcacg caccaggcgc tgcagcgccg
      781 ccgcatccca ggcacgcagc ggatacgtga cacccgcggg ggccgcgatg ccggcgagga
      841 gcttgccgat cgggttgtat gcgccgatga ggctccaggc cgagcggctc gagtcggcca
      901 gcttgcggtc cgcggccacg acgccagggc cgggcgcgag gtccatcagc cgctgcgcct
      961 gctgcgcgaa cagattcacc gtggcgttga gcttgaagaa gtgcccgctg acttcgctcc
     1021 ccagccgatc ggtccagctg cgcggtgcgc cgctgcgcgc ggcctgcggg gtccagccga
     1081 tcgtgtacag gtactgcgtc tgctcgagga gcgatgcctg cacgcgaaac tccgcggcga
     1141 acgccgcgcc gaggtcgaag tccctgagag cgaagagcgg cgcggcggtg tccgcgtcgt
     1201 cgggccccac gggcaccgcg gcatgcggat cggcgatcat gtcggcgagc acgaggtagt
     1261 cggactcgag ataggcgagt gccagcatct tcgagatcac cagtccctgc ccggtcagca
     1321 cgcgccgcca caggccgacg tcggctacca gctcatcgag cgccgcgcgc tgcgcgcgcg
     1381 cgctgccgct gcgcagccgc agcaccgtgt cctcgaggaa caggtagcgc acctcggtgg
     1441 gaacgaagat cgccggagcc aggtagctcg gccgcgcgct ctcgaagtag ccgggcaggc
     1501 gctcgagagc tagatagcgc tgatagagtt cgccgttctg cgcggtcagc gcctcgatct
     1561 gcgcgcgatg cgggggcacg tcctcccaca gcgagccggt cagcggctgc acgaactgcg
     1621 cctcaccctg gaactcgagc gcgtgcgggt ccttgaacgt cagggagtcg agggcctggg
     1681 gcgagggatc gtgcagcacc gcatcgaggt gctcgttgta gt
//
LOCUS       DATLKL010000554         2331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_175653, whole genome shotgun sequence.
ACCESSION   DATLKL010000554 DATLKL010000000
VERSION     DATLKL010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175653"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VM713_07450"
     CDS             <1..344
                     /locus_tag="VM713_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HVH84133.1"
                     /translation="DFSAFRSAECQARSPVRRLTRLTVERRGDWIVIEAVANAFLHHM
                     VRNIAGLLIAVGKGDEPPDWARTVLEGRDRTRSAATAPAAGLYLWEVRYPEAFGLPAP
                     PPDRGPGADQL"
     gene            394..1257
                     /gene="accD"
                     /locus_tag="VM713_07455"
     CDS             394..1257
                     /gene="accD"
                     /locus_tag="VM713_07455"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HVH84134.1"
                     /translation="MTWFDKIMPSRIKTERRTRSVPEGLWKKCPACDAVLYAAELERN
                     LYVCPKCGHHMRIGARERLERFLDADSGKEIGASIAPEDPLKFRDSKRYRDRLAQAQK
                     ATGESDALVVLSGTLHALPIVACAFEFQFLGGSMGSVVGERFKRGVDCCIEERRPLVC
                     FTASGGARMQEALYSLLQMAKTAAALSRLAQAHLPFVSVLTDPTTGGVSASLALLGDL
                     NIGEPRALIGFAGPRVIQQTVRETLPEGFQRSEFLLEHGAIDMIIDRRDMRDRIAALL
                     RLLLKLPAAAA"
     gene            1286..2296
                     /locus_tag="VM713_07460"
     CDS             1286..2296
                     /locus_tag="VM713_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HVH84135.1"
                     /translation="MRTLAEWLELQDSVHPRTIDLSLERVTAVAERLGLIAPAFRVVT
                     VGGTNGKGSVAAHAEALLRAAGTATGLFTSPHLVRYQERIRVNGAEAADAELIEAFER
                     IEAARATDTLTFFEYNTLAALLIFERRAVEVAVLEVGLGGRLDATNLVAADVAVIASV
                     GLDHREWLGESLEAIGAEKAGILRAGRPAVLGTAEMPASVYARIATLGTRALVAGRDF
                     TWEVAGERWCYRSARLALEDLAPSALAGSIQYRNAATAIAAVAALAGSMPLPRALERS
                     TANIALAAVHLAGRFQIVPGEIEWILDIAHNEPAARVLACICASGRCRAGRPGGGDAP
                     SQ"
BASE COUNT          328 a          846 c          813 g          344 t
ORIGIN      
        1 acgacttcag cgccttccgc tctgcggaat gccaggccag gtcgccggtg cggcgcctga
       61 cgcgcctcac ggtcgagcgg cgcggcgact ggatcgtcat cgaggcggtc gccaacgcct
      121 tcctgcacca catggtgcgc aacatcgccg gcctcctgat cgccgtcggc aagggcgatg
      181 agccgcccga ctgggcgcgc accgtgctcg aggggcgcga ccgcacccgc agcgccgcca
      241 cggcgccggc cgcagggctc tatctctggg aggtccgcta cccggaggct ttcggactgc
      301 ccgcacctcc cccggaccgg gggccgggcg ccgatcagtt atgatcgaag cgcggccgcg
      361 ggatcgccgc ggcgcctgcc gagagtgggc tcgatgacct ggttcgacaa gataatgccc
      421 tcgcgcatca agaccgagcg tcgcacgcgc tcagtgcccg agggactgtg gaagaagtgt
      481 ccggcgtgcg acgcggtgct ctacgccgct gagctcgagc gcaacctcta cgtgtgcccc
      541 aagtgcggtc atcacatgcg catcggcgcg cgcgagcgcc tcgagcgctt tctcgacgcg
      601 gactcaggca aggagatcgg cgcgtcgatc gccccggagg acccgctcaa attccgcgac
      661 agcaagcgct accgcgaccg cctcgcgcag gcgcagaagg ccaccggaga gagcgatgcg
      721 ctggtcgtgc tgtccggcac gctgcacgcg ctgccgatcg tcgcctgtgc gttcgagttc
      781 cagttcctcg gcggctcgat gggctcggtg gtcggcgagc gcttcaagcg cggcgtcgat
      841 tgctgcatcg aggagcgccg cccgctcgtc tgtttcacgg cgagcggcgg ggcgcgcatg
      901 caggaggcgc tgtactcgct cctgcagatg gcgaagaccg ccgccgcgct ctcgcgactc
      961 gcgcaggcgc acctgccctt cgtctcggtc ctgaccgatc cgaccaccgg cggcgtgtcg
     1021 gcgagcctcg cgctgcttgg ggacctcaat atcggcgagc cgcgcgcgct catcggtttt
     1081 gcgggcccgc gcgtgatcca gcagaccgta cgcgagacct tgcccgaggg ctttcagcgc
     1141 tccgagttcc tcctcgagca tggcgccatt gacatgatca tcgaccggcg cgacatgcgc
     1201 gaccgcatcg cggcgctcct gcggctgctg ctgaagctgc cggccgcggc cgcctgaacc
     1261 ggacggcagg cgcttcgcaa cgcggatgcg cacgctcgcc gagtggctcg agctccagga
     1321 ctcggttcac ccgcgcacca tcgacctctc gctcgagcgc gtcacggcgg tcgccgagcg
     1381 gctcgggttg atcgcacccg cattccgcgt cgtgacagtc ggcggcacca acggcaaggg
     1441 ctcggtggcc gcgcatgccg aggcactgct gcgggcggcg ggcacggcga ccggcctgtt
     1501 cacctcacct cacctggtgc gctaccagga gcgtatccgc gtgaacggcg ccgaggccgc
     1561 cgacgccgag ctcatcgagg cgttcgagcg catcgaggcc gcacgcgcca ccgacacgct
     1621 gaccttcttc gaatacaaca cactggcggc gctgctgatc ttcgagcgcc gcgcggtcga
     1681 ggtcgcggtg ctcgaagtcg gcctcggcgg gcgcctcgat gccaccaacc tggtcgcagc
     1741 cgacgtcgcc gtcatcgcct ccgtcgggct cgaccatcgc gagtggctcg gcgagagtct
     1801 ggaggcgatc ggcgcggaga aggccggaat cctccgcgcc ggccgaccgg ccgtgctcgg
     1861 caccgccgag atgcccgcga gcgtgtacgc gcgcatcgcg acgcttggga cacgcgcgct
     1921 cgtggcgggc cgcgatttca cctgggaggt cgccggtgag cgctggtgct atcggagtgc
     1981 gcggctggcg ctcgaggatc tcgcgccctc cgctcttgcg ggctccatcc agtaccgtaa
     2041 cgccgcgacc gccatcgctg cagtcgcggc gctggcaggc tcgatgcccc ttccccgagc
     2101 tctggagcgc agcacggcga atatcgcgct cgccgccgtg cacctcgcgg gccgcttcca
     2161 gatcgtcccg ggcgagatcg agtggatcct cgacatcgcg cacaacgagc ccgcggcacg
     2221 cgtgctcgcc tgcatctgcg cgagcgggcg ctgccgggca ggacgaccgg ggggcgggga
     2281 cgcaccctcg cagtgacggg cgtgctcgcc gacaaggacg cggcagggat c
//
LOCUS       DATLKL010000555         3337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_198442, whole genome shotgun sequence.
ACCESSION   DATLKL010000555 DATLKL010000000
VERSION     DATLKL010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198442"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="VM713_07465"
     CDS             <1..1408
                     /locus_tag="VM713_07465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HVH84136.1"
                     /translation="VSVVVSGLWKQRGPDQSAPGQYTSRVIAQYDVLAAGTPLAPLLA
                     AGTASIDDPYGHGTHVSSIIASSGLATTGNFQGVAPGVNLVSVRVLDANGVGMYSNVI
                     SGIQWVIAHKSSYHIRVMNLSLSAPPHSFYWQDPLNQAVMAAWQAGIVVVVAAGNAGP
                     KPMTIGVPGNVPYVITVGAVTDNYYPMQPSQYKLTSFSSAGPTYEGFVKPEVVAMGGH
                     ILAYAPNNGTLAEEFPQWVHLPYDDFSMSGTSMAAAVTSGTVALMLEVSPSLKPDDIK
                     CRLMTGAHPAVKSDGTLAYTVFQQGAGLVNAHDAVYSTASACANQGLNVTNDLLGIQH
                     YGGRANYNPKTGEYYIMATTQGSSSGGLLGPLGGVVGGLVGGLATVASAVPVVGPTLG
                     SLLWGVAELGDGLLWGGGYTSGSGYSWSSGYAWSSGYTWANGYAWSSGYAWSSGYPWS
                     SGYAWSSAYSGVDLPRQQ"
     gene            1451..>3337
                     /locus_tag="VM713_07470"
     CDS             1451..>3337
                     /locus_tag="VM713_07470"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HVH84137.1"
                     /translation="MQFRHLSAVVGALLALCAPAPPLVAAMPVPPVRSLYFEHLTVRE
                     GLSHSTVEGFLQDSEGYLWIATESGLDRYDGNSFRIYRRERGNPHALASDTIWTIAED
                     ARSDLWLATDGGGVAHWQRRTDQFELFRHDPARPDSLASDAVRTLLIDASGSIWAGTL
                     DRGLDLLDPATGSARHFRHRDDDPHSLAADAVYALYRDRAGRIWIGTNGGLSHYDQAT
                     GGFINYGKSGDGAGLSDLQVRAIREDHTGALWIGTMRGGLDRLEPGSGRVTSFRHDPA
                     NPRSLSHDRVSAILEDGTQRLWVGTPDGLNLFDRTTESFVRYGNDPTNSQSLRSSYVM
                     FNALYQDRGGVLWVGTRGGGASHWNPDSWSLGNYRSATFHDTEVSSFADDGAGRVWVG
                     TIGSGLVELDAHGGGERRYGHDTGPLRLSDDRVMALLYDHRGTLWVGTMTGGLDQVEF
                     ARGKVQVWKASPGDPHALPADGVMTLYQDHLDNVWVGTFGGGIASITPASGQLTRYPF
                     GDEGTSSLSDGRASAIAEDALGNLWIGTVGGGLNLLERASGRFYHYRRDDKDPASLSD
                     DAVYSLHVDRRGDVWVGTRSGGVDHVVGSSAAPQAVHFENYSSALPSRAVYGIESDGE
                     GWLWL"
BASE COUNT          550 a         1148 c         1109 g          530 t
ORIGIN      
        1 ggtctcggta gtggtttctg gcctgtggaa gcagcgcgga ccggatcaga gcgctccagg
       61 ccaatacacg agccgcgtca tcgcgcagta cgacgtgctc gcggcgggca caccgctggc
      121 gccgctcctg gccgccggca ccgccagcat cgacgacccc tacggccacg gtacccacgt
      181 cagctcgatc atcgccagca gcggcttggc gaccacgggc aattttcagg gcgtcgctcc
      241 cggcgtgaac ctggtgagcg tgcgcgtact cgacgccaac ggcgtgggga tgtactcgaa
      301 cgtgatcagc ggcatccagt gggtcatcgc ccacaaatcg agctaccaca ttcgggtgat
      361 gaacctgtcc ctgagcgccc cgccgcactc cttctactgg caggacccgc taaaccaggc
      421 ggtgatggcc gcctggcagg ccggcatcgt ggtggtcgtg gcggccggca atgccggacc
      481 caagcccatg accatcggcg tacccggcaa cgtgccgtac gtgatcaccg tgggtgcggt
      541 caccgacaac tactacccga tgcagccgag ccagtacaag ctcacctcat tctcatccgc
      601 tggtcccact tacgagggct tcgtgaagcc ggaagtcgtg gccatgggcg gacacattct
      661 cgcctacgca cccaacaacg ggacgctcgc cgaggagttc ccacagtggg tgcatctgcc
      721 ctacgacgac ttcagcatgt cgggcacgtc catggcggcg gcggtgacca gcggcaccgt
      781 ggcgctcatg ctcgaagtga gcccgtcact caagccggac gacatcaagt gccggctgat
      841 gacgggcgcg cacccggcgg tcaagtccga tggcacgctc gcctacacgg tgttccagca
      901 gggcgcggga ctcgtcaacg cgcacgatgc cgtctacagc accgcgagcg cttgcgccaa
      961 tcagggcctg aacgtcacca acgacctgct cggcatccag cattatggcg ggcgtgccaa
     1021 ctacaacccg aagaccggcg agtactacat catggccacc acccagggct cgagttcggg
     1081 cgggctcctc ggtccccttg gcggtgtggt cggcggtctg gtcggcggac tggcgacggt
     1141 cgcctcggcg gtgccggtgg tgggcccgac cctcggaagt ctcctgtggg gtgtcgcgga
     1201 gctcggagac ggactgttgt ggggcggtgg ctatacctcg ggtagcggtt actcctggag
     1261 cagcggctat gcctggagca gcggttatac ctgggccaac ggctatgcgt ggagcagcgg
     1321 ctacgcctgg agcagcggct atccctggag cagcggttac gcctggagca gcgcctactc
     1381 gggcgtcgac ctcccgcgtc agcagtaggc ttccggccgg cgcaaccgcg gagcgcgcac
     1441 cagaggcgtc atgcaattcc gccacctgag tgccgtcgtc ggcgcactgc tcgcactgtg
     1501 cgcgccggcg ccaccgctcg tcgccgcgat gcccgttcca cccgtgcggt cgctctactt
     1561 cgagcacctg acagtgcgcg aaggactctc gcacagcacc gtcgagggct tcctacagga
     1621 ctcggagggg tacctgtgga ttgcgaccga aagcgggctc gaccgctacg acggcaactc
     1681 cttccgcatc tatcggcgtg agcgcggcaa cccgcacgcg ctcgcgagcg ataccatctg
     1741 gacgatcgcc gaggacgcac gctccgatct atggcttgcc accgacggag gcggagtcgc
     1801 ccactggcag cgccgcaccg atcagtttga gctcttccgg cacgatccgg cgcggcccga
     1861 ctcgctggcg agcgacgcag tgcggacgct cctgatcgac gcctccggca gcatctgggc
     1921 tggaacactc gatcgcggcc tagacctgct cgatcctgcc accggctccg cgcgccactt
     1981 tcgccaccgc gacgacgacc cgcactcact ggccgcggac gccgtgtacg ccttgtaccg
     2041 cgaccgcgcc ggacgcatct ggatcggcac caacggcggg ctcagccact acgaccaggc
     2101 cacgggtggt ttcatcaact atggcaagtc gggcgatggc gcgggcctgt ccgacctcca
     2161 ggtgcgcgca atccgcgagg atcacacggg agcgctgtgg attggcacca tgcgcggcgg
     2221 gctcgatcgc ctggagccgg gctccggccg cgtgacctcc ttccgccacg atcccgccaa
     2281 cccacgctcg ctcagccatg accgcgtgag cgcgatcctc gaggacggca cgcaacggct
     2341 ctgggtcggg acccccgacg ggctgaacct tttcgatcgc acgaccgaga gcttcgtgcg
     2401 ctacggcaac gatcccacca actcccagag cctgcgcagc agctatgtca tgttcaatgc
     2461 cctgtaccag gacaggggcg gtgtcctgtg ggtcggtacg cgcggcggcg gcgccagcca
     2521 ttggaatccc gacagctggt cgctcggaaa ttatcgcagc gctaccttcc acgacaccga
     2581 ggtcagcagc ttcgctgacg atggggctgg gcgtgtctgg gtgggcacga tcggctcggg
     2641 gctcgtcgag ctcgatgcgc acggcggtgg cgagcggcgc tacggacatg acaccggtcc
     2701 gctgcgcctg agcgacgacc gcgtgatggc gctgctctac gaccaccggg gcacgctctg
     2761 ggtcggcacc atgacgggcg ggctcgacca ggtggaattt gcgcgcggca aggtgcaggt
     2821 ctggaaagcc tcgccgggcg atccccatgc gttgcccgcc gacggcgtga tgacgctcta
     2881 ccaggaccac ctcgacaacg tatgggtggg cactttcggc ggcggcatcg ccagcattac
     2941 gcccgccagc ggtcagttga cccgctatcc gttcggcgac gagggcacct cctcgctcag
     3001 cgacggacgc gccagcgcga tcgccgagga tgcgctcggc aacctctgga tcggcaccgt
     3061 gggcggaggc ctgaacctcc tcgagcgcgc gagcggacgc ttctatcact accgccgcga
     3121 cgacaaggat cccgcgagcc tctcggacga cgcggtgtat tccctgcacg tggatcgacg
     3181 gggcgacgtg tgggtgggaa cgcgctcggg tggcgtcgac catgtggttg gcagctcggc
     3241 cgcgccgcaa gcggtgcact tcgagaatta cagcagcgcg ctgccgagcc gcgcggtcta
     3301 tggcatcgag tccgacggcg agggctggct gtggctg
//
LOCUS       DATLKL010000556         1507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_6387, whole genome shotgun sequence.
ACCESSION   DATLKL010000556 DATLKL010000000
VERSION     DATLKL010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6387"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            484..>1507
                     /locus_tag="VM713_07475"
     CDS             484..>1507
                     /locus_tag="VM713_07475"
                     /inference="COORDINATES: protein motif:HMM:NF019902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84138.1"
                     /translation="MGPYGADSDSHGSNATFIDTLSSFSRLHRGQRWEGTFGDFLANI
                     LPTHPSALARTSHEYIWDMLRWHGRAAASDAAENVRARELFRHELFGIDEPLSRLVDY
                     FKAAAAGSDVGRRLLLMLGPPSGGKSTVAILLKRGLEEYSRTDEGALYAIRGSPLREN
                     PLNLIPTSLRAEFRQRYGIAVQGELSPWARDFVERDYEGDFVRVPVERVFLAEASRVG
                     IGTYAPHDPTTADIADLVGSVDLAKVAQIGDEGDPRAWSWSSAVYAASRGVLEMIEIL
                     KVKREFLYLLLTLTQEKNVKVSRFPLIHLDETILAHTNLAEFHKFLQEKENEALLDRM
                     VIIKIPY"
BASE COUNT          257 a          502 c          481 g          267 t
ORIGIN      
        1 gttagttcac aataacgata gggcgcgtct taacttggcg acggcgatcg ccaaactctg
       61 aagcactgct cgctcctcgc ccggtgcttc agtttcggac cgccgggcgc tcaccgggac
      121 gcgtgcacgg cggcgcgcca acaggccgcg ccctcaggta gcaccaggga accggcagga
      181 accgcatcgg ccgattgcgg cgcgcggact cgccagggtg aggcgcgccg cgagctttgc
      241 aatcagctac ttaagagctc gtccccttct gcgcgccgat tccgcgaggt gtgatggcac
      301 ccagaccctc tggcgtgaac aatccgttgc tgtgacactc ttaacgtaat catgccacga
      361 ggattggaag gccgtcactt tttgtggggg gcgttgggac taagttgatg ccaggcggat
      421 cggcctggcc gcgcgctggc atccagggac tggtgactac gcgctcaacg gagggtaagc
      481 acgatgggac catacggagc tgactcggat tctcacggta gtaacgccac cttcatcgac
      541 acgctgagct ccttcagccg gctgcaccgc gggcagcgct gggaaggcac cttcggggat
      601 ttccttgcca acatcctgcc gacccatccg agtgcgctgg cgcgcacgag ccacgaatac
      661 atctgggaca tgctgcgctg gcacggccgt gctgcagctt ccgacgccgc cgagaacgtc
      721 cgcgcgcgcg agctcttcag gcacgagctg ttcggcatcg acgagccgct gtcgcggctg
      781 gtcgattact tcaaggcggc cgccgcgggc tcggatgtcg gccggcgtct gctcctgatg
      841 ctcgggccgc cctcgggcgg caagtccacc gtcgcgatac tcctcaagcg gggcctcgag
      901 gaatacagcc gcacggatga gggcgcgctc tatgccattc gcggctcgcc gctgcgcgag
      961 aatccgctga acctcattcc caccagcctg cgcgccgagt tccgccagcg ctatggcatc
     1021 gccgtgcagg gcgagctctc gccctgggcg cgtgatttcg tcgagcgcga ctacgagggc
     1081 gacttcgtgc gcgtgccggt cgagcgggtg ttccttgccg aggcctcgcg tgtcgggatc
     1141 ggcacctacg cgccgcacga tcccaccacc gcggacatcg ccgatctcgt cggctcggtc
     1201 gatctcgcca aggtggcgca gatcggcgat gagggcgatc cgcgcgcctg gtcgtggtcg
     1261 agcgcggtgt acgccgccag ccgcggcgtg ctcgagatga tcgagatcct gaaagtgaag
     1321 cgcgagttcc tctacctgct gctcaccctc acccaggaga agaacgtcaa ggtgtcgcgc
     1381 ttcccgctga ttcacctcga cgagaccatc ctcgcccaca ccaacctcgc cgagttccac
     1441 aagttcctgc aggagaagga gaacgaggcg ttgctcgatc gcatggtgat catcaagatc
     1501 ccctatg
//
LOCUS       DATLKL010000557         1894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_198467, whole genome shotgun sequence.
ACCESSION   DATLKL010000557 DATLKL010000000
VERSION     DATLKL010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198467"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..800)
                     /locus_tag="VM713_07480"
     CDS             complement(237..800)
                     /locus_tag="VM713_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVH84139.1"
                     /translation="MRKLLALLALALGAGAAHADNGRLYVGAGIANDNLRDVTATNSN
                     LNSTNWKVWAGVRPIPFLAVEANYIDLGSQNVTSMFVSGTSSTHLSYKAFTGYAVGYV
                     PLPVPFLDVFGKVGLARWTASGGSTLTGPGAGFFSLSDNGTQFAWGAGGQVHIGNVGA
                     RLEYENFSVRNTNGANLVSLSVFLNLF"
     gene            complement(929..1465)
                     /locus_tag="VM713_07485"
     CDS             complement(929..1465)
                     /locus_tag="VM713_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVH84140.1"
                     /translation="MRRVLPVIVLALGASAAHADTGSFYLGAGVSKDKVSGIVNGATD
                     IDTTAWKVLAGLRPISVVAIEADYLNLGSQTNTFVTTTTHSDAKAFAGYAVGFLPIPL
                     PFLDVFGKAGLARWKLHGSAASPLTSFSTSGTEFAWGVGTQVHVENFGARLEYENFKI
                     PSTNGANVVSLDVYVNFF"
     gene            complement(1598..>1894)
                     /locus_tag="VM713_07490"
     CDS             complement(1598..>1894)
                     /locus_tag="VM713_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HVH84141.1"
                     /translation="GNTQLRQYAERSAINAPMQGTAADIIKRAMIALDAWCRRSGSPA
                     RLIMQVHDELVFEVRTEAVEEVTRAVRERMGGAATLSVPLRVDVGTGANWDEAH"
BASE COUNT          338 a          584 c          633 g          339 t
ORIGIN      
        1 cttgaaataa aaaaccccgg cggcttatga ggccgccggg gcagactaac ccggcacagg
       61 tctcgtgaac cgggtcgaat tacccgcctc agggagggaa ggcggggagt gactagcact
      121 cgatgaatta gatccgggca ccccgcgaaa aattccctcc ggaccgcaaa aaaatctcgt
      181 tacagatcat aaatttgctt aacgccttgt atcgccagcg ccacagaggt gccgcgtcag
      241 aacaggttca ggaaaacact gagcgagacg agattggcgc cgttcgtgtt gcggacgctg
      301 aagttctcgt attcgaggcg ggcgccgacg ttgccgatgt gcacctgacc gccggctccc
      361 caggcgaact gagtgccgtt gtccgataag gagaagaacc cagcgccggg gccggtgagc
      421 gtgctgccgc cgctcgcggt ccagcgcgcg agtccgacct tgccgaagac gtcgaggaac
      481 gggacgggga gcggcacgta accgaccgcg tatccggtga aggctttgta gctgaggtgc
      541 gtgctgctcg tgcccgagac gaacatcgac gtgacattct ggctgccgag gtcgatgtaa
      601 ttggcctcga cggcgaggaa tgggatcggg cgcacacccg cccacacctt ccagttggtg
      661 ctgttcaagt tggaattggt ggcggtgacg tcccgcaggt tgtcattggc gattccggcg
      721 ccgacataga gccttccatt gtcggcgtgt gcggcgcctg cgccgagtgc cagcgcgagc
      781 agagccagga gtttgcgcat acgagtcctc tccttcagcg tgttgaattc cctgataact
      841 gacttgcggc gccggccttg acacggggcc gcggcgtgcc gccgcgcggc agcgccgcgc
      901 gcgaagtcgc aggcttgggt cggcgcgcct agaagaaatt cacgtacacg tcgagcgaca
      961 cgacgttcgc gccgttggtg ctcgggatct tgaagttctc gtactcgagt cgggcgccga
     1021 aattttcgac gtgcacctgg gtgccgaccc cccaggcgaa ttcggtgccg ctggtcgaga
     1081 agctcgttag cggcgacgcc gcgctgccgt ggagcttcca gcgcgcgagc cccgccttgc
     1141 cgaagacgtc gaggaacggc agcgggatcg gcaggaagcc gaccgcgtag ccggcgaacg
     1201 ccttggcgtc cgagtgggtg gtggtggtca cgaacgtgtt ggtctgactg ccgagattga
     1261 ggtagtcagc ctcgatggcg accacgctga tgggcctcaa gccggccagc actttccagg
     1321 cggtcgtgtc gatgtcggtg gcgccgttga cgatgccgct cactttgtcc ttcgagacgc
     1381 cggcgccgag atagaacgat ccggtgtcgg cgtgtgcggc gctcgcgccg agcgcgagaa
     1441 cgataacggg aaggactctg cgcatgctga acctcctgtt ccgagccgct aaccttacac
     1501 aatcgcgacc gcggtgtttg actgttgcaa cgcggcgaca cacgggcggc gacccgatgc
     1561 gatgtcaaga ctcgttcagt agaggttcag gaagacttca gtgcgcctcg tcccagttgg
     1621 cgcccgtgcc gacgtcgacg cgcagcggca cactcagggt tgccgcgccc cccatgcgct
     1681 cgcgcaccgc ccgcgtcact tcctcgactg cctcggtgcg cacctcgaac acgagctcat
     1741 cgtgcacctg catgatgagc cgagcggggg aaccggaccg gcggcaccat gcatccagtg
     1801 cgatcatggc ccgcttgatg atgtcggccg ccgtgccctg catcggcgcg ttgatggcgc
     1861 tgcgctcggc gtactggcgc agctgcgtgt tgcc
//
LOCUS       DATLKL010000558         2126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_17771, whole genome shotgun sequence.
ACCESSION   DATLKL010000558 DATLKL010000000
VERSION     DATLKL010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17771"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..2126)
                     /gene="rpoB"
                     /locus_tag="VM713_07495"
                     /pseudo
     CDS             complement(108..2126)
                     /gene="rpoB"
                     /locus_tag="VM713_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003232409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
BASE COUNT          295 a          738 c          678 g          415 t
ORIGIN      
        1 taatactcct cgacgagggc ttcctcgccc tcgaggttga tgccgagcgc ccgtgccgtc
       61 tcggcgatgt cttcctcaat ctccttgagc tcgatctctt tctggtcctc gctcagcacc
      121 ttgacgtcga ggcagaggct ctggagctcc ttgacgagca ccttgaagga ttcaggcact
      181 cctggctcca ggatgctttc gcccttcact atcgcctcgt acgttttcac gcggccgatc
      241 acgtcgtccg acttgacggt cagcatctcc tgcagcgtgt aggaggcgcc gtaggcctcg
      301 agcgcccaca cctccatctc cccgaagcgc tggccgccga actgcgcctt gccgcccagc
      361 ggctgctgcg tgaccagcga gtacggcccg gtcgagcgcg cgtgcatctt gtcgtcgacg
      421 aggtgattca gcttcagcat gtacatgtag ccgaccgtca cctgccgctc gaagcgctcg
      481 ccggtgcggc cgtcgtagag gtacgtctgg ccgttcaagg ggagctcggc gagcttgagc
      541 atgcgcttga tctcctgctc gctcgcaccg tcgaacaccg gcgtcgccat cggcacgccg
      601 cccgcgaggt tcctggcgag ctgcgtgagg tccgcatcgc ccaggctgtc gatctcctcg
      661 cgctgaccgc cggtctcgtt gtagatctgc ttgacgaaag cgcgcagctc ggaggccgcc
      721 gcctgcgctt ccagcatccg cccgatcttg aggccgacgc ccttggccgc ccagccgagg
      781 tgggtctcga gcacctgccc gacgttcatg cgcgagggca cgcccagggg gttcagcacc
      841 acgtcgaccg gggtgccgtc ctcgaggtac ggcatgtcct cgaccggcac gatggtcgag
      901 atcacaccct tgttgccatg gcggccggcc atcttgtcgc ccggctgcac gcggcgcttc
      961 accgcgaggt acaccttgac catcttgagc acgccgggag cgaggtcgtc gccggcggtg
     1021 atcttggcgc gcttgtcatc gaggcgcttc tcgaacgcct tgcgctgcag cttcaagcgc
     1081 tccgaggcct gctcgagctg gctgttcgcg tcctcgtcct cgaggcggat ctcgaaccac
     1141 tcctcgcgcg gcacctcatc gaggtactcg ctggcgatgc gggtgccgct cttgagcttc
     1201 ctcggcccgc ccgcggcgct ctgtccgacc agcatgccgc gtacgcgctg gaacaggtcg
     1261 tcctcgagga tgcgctgctg gtcggcgaag tccttgcgga cccgctcgat ctcggccttc
     1321 tcgatcgaga gcgcgcgcga gtccttctcg acgccgtcgc gcgtgaagac gcgcacgtcg
     1381 atcacggtgc cgtccatgcc gggcggcacg cgcagtcccg tgtccttcac gtccgacgcc
     1441 ttctcgccga agattgcgcg cagtagcttc tcttccggcg tgagctgcgt ctcgcccttg
     1501 ggcgtcacct tgccgaccag gatgtcaccg gccttgacct cggcgccgat gaaggcgatg
     1561 cccgcctcat cgagcttgcc gagcgccgcg tcgccgacat tggggatgtc ggcggtgatt
     1621 tcctcgggtc cgagcttggt gtcgcgcgcg acgcaggtga gctcctcgat atggatcgtc
     1681 gtgaagcggt cctcctccac cacgcgctcg gagatgagga tcgagtcctc gaagttgtaa
     1741 ccattccagg gcatgaaggc gacgagcgcg ttcttgccga gcgcgagctc gcccagatgc
     1801 gtcgacggac cgtctgcgat gatgtcgccc tgcttgacca cgtcgcccac cttcaccagc
     1861 ggacgctggt tgatgcaggt gttctggttg gagcgctgga acttctgcag ccggtagatg
     1921 tcgacgcccg acttcgaggg gtcgagctcc tcggtcgcgc ggatgacgat acgggtggcg
     1981 tcgacctgat cgacgacgcc ggtgcggcgc gcggcaatcg ccgcgccgga gtcgcgggcg
     2041 acgacacctt ccatgccggt gccgacgaac ggcgcttcgg cgcgcaccag cggcaccgct
     2101 tggcgctgca tgttggagcc catcag
//
LOCUS       DATLKL010000559         4122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_198513, whole genome shotgun sequence.
ACCESSION   DATLKL010000559 DATLKL010000000
VERSION     DATLKL010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198513"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /gene="secA"
                     /locus_tag="VM713_07500"
     CDS             complement(<1..1654)
                     /gene="secA"
                     /locus_tag="VM713_07500"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HVH84142.1"
                     /translation="MLDFLTRIFGSRNERLLRGYARYVRAANELEPRIESLSDEALRG
                     KTDEFRARLKEGVSLEELLPEAFAVVREAARRTLKMRHFDVQLIGGIVLHKGMIAEMR
                     TGEGKTLVATLPAYLNALTGEGVHIVTVNEYLAQRDADWMGPVYRFLGLTVGVIKNAQ
                     TPAEKRAAYACDITYGTNNEFGFDYLRDNLAFRLEDRVQRSLSFAVVDEVDSILIDEA
                     RTPLIISGPAEESTELYLRINQLVPRLTRQKEEEGPGDFAVDEKTKQVHITESGHEHV
                     ERLMADAGLLREGESLYDPANIRLMHHLNAALRAHALYKRDVEYIVRNGEIIIVDEFT
                     GRTMIGRRWSDGLHQAVEAKEGVRVREENQTVASITFQNYFRIYKKLSGMTGTADTEA
                     PEFLQIYGLEVVVIPTHRPMVRKDNSDFVYLTQKDKFQAIIEDIRDCVEREQPALVGT
                     TSIETSEYLSGLLQKEDVAHQVLNAKQHEREAHIVAQAGRPGTVTIATNMAGRGTDIV
                     LGGNLEAELAAAGELDEAARQRLRTEWQARHDRVVSAGGLHII"
     gene            complement(1799..2713)
                     /locus_tag="VM713_07505"
     CDS             complement(1799..2713)
                     /locus_tag="VM713_07505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVH84143.1"
                     /translation="MNVILLSRRDGRARQFNLARPRTLGGLLGLTVLGVAGVFTLGWQ
                     LGKGVHREIALAETTRFAAVLAQQKQEIAGLKQQLQLRIDAMAMRIGEVNAHIIRLNA
                     LGKRLTEMADIDSREFDFDRSPPSGGPEGEGVSAQIPDLSAMLARLEQRADLRESQLA
                     ALENVILTRELKEEIHPEGRPVATGYISSYFGERPDPFDGREAFHKGVDFAGALGSDV
                     VAVAAGVVTWAGERSGYGKLIEINHGDGYATRYAHNSRTLVSVGQTVKRGEAVALMGS
                     TGHSTGPHVHFEVLHNGRQVDPLSFIGR"
     regulatory      complement(2817..2900)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2953..3276
                     /locus_tag="VM713_07510"
     CDS             2953..3276
                     /locus_tag="VM713_07510"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HVH84144.1"
                     /translation="MRNRTVAGSRQPDSVKDLLARRAPSLMRVTTQAARARFWSDWLS
                     QHLPAEVGTRVSGVVERDGKLVIFAESAAWSARLRYLVQELEPQILAAARGLTAVAVR
                     VRPRD"
     gene            complement(3273..>4122)
                     /gene="lpxC"
                     /locus_tag="VM713_07515"
     CDS             complement(3273..>4122)
                     /gene="lpxC"
                     /locus_tag="VM713_07515"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HVH84145.1"
                     /translation="TLKPADPDEGITFRRTDLAQPVDIRASADNVGDTMLGTTLGQGE
                     SRVSTVEHLLSAFAGLGIDNAVVDLSAAEVPIMDGSAGPFVFLLQSAGIEEQRRPKRF
                     IRVKKRLRVEDGDKWAQFDPFDGFKVNFEIEFNHPIFKRRSQRASMDFSTTSFLKEVS
                     RARTFGFMRDLETLRARNLALGGNLDNAIVLDDFRVLNEDGLRYEDEFVKHKILDAIG
                     DLYLLGHSLIGEFSGHKSGHALNNRLLRVLLADASTWEEVVFQSYKDAPISYIEAPAL
                     PGSRRS"
BASE COUNT          647 a         1360 c         1384 g          731 t
ORIGIN      
        1 cgatgatatg cagcccgccg gcgctcacga ccctgtcgtg acgcgcctgc cactcggtac
       61 gcagccgctg gcgggcggcc tcgtcgagct cgcccgcggc ggcgagctcg gcctcgagat
      121 tgccgcccag cacgatgtcg gtgccgcggc cggccatgtt ggtggcgatg gtgaccgttc
      181 cgggccgtcc cgcctgggcg acgatgtgcg cctcgcgctc gtgctgcttg gcgttcagca
      241 cctggtgcgc gacgtcttcc ttctgcagga ggcccgacag gtactccgag gtctcgatcg
      301 acgtggtgcc gaccagcgcc ggctgctcgc gctcgacgca gtcgcggatg tcctcgatga
      361 tcgcctggaa tttgtccttc tgcgtgaggt agacgaagtc ggagttgtcc ttgcgcacca
      421 tcgggcggtg cgtcgggatg accaccacct cgaggccgta gatctgcagg aactcgggcg
      481 cctcggtatc ggccgtgccg gtcatgcccg agagcttctt gtagatgcgg aagtagttct
      541 ggaaggtgat cgaggcgacc gtctggttct cctcgcgcac ccgcacgccc tccttggcct
      601 cgaccgcctg gtgcagcccg tcggaccagc gccggccgat catggtgcgc ccggtgaact
      661 catcgacgat gatgatctcg ccgttgcgca cgatgtattc gacgtcgcgc ttgtagagcg
      721 cgtgggcacg cagcgccgca ttcaggtgat gcatgaggcg gatgttggcg gggtcgtaga
      781 gactctcgcc ctcgcgcagc agccccgcgt ccgccatgag ccgctcgaca tgctcgtggc
      841 cggactcggt gatgtgcacc tgcttggtct tctcgtcgac cgcgaagtcg cccggcccct
      901 cctcctcctt ctgccgcgtg aggcgcggca ccagctggtt gatgcgcagg tagagctcgg
      961 tgctctcctc ggcgggaccg gagatgatga gcggggtgcg cgcctcgtcg atcaggatcg
     1021 agtcgacctc gtccacgacg gcaaagctca aggagcgctg cacccggtcc tcgagcctga
     1081 aggcgaggtt gtcgcgcaga taatcgaagc cgaactcgtt gttggtgccg taggtgatgt
     1141 cgcaggcgta ggcggcgcgt ttctcggccg gcgtctgcgc gttcttgatc acgccgaccg
     1201 tgaggccgag gaagcggtac accgggccca tccagtccgc gtcgcgctgc gccaggtact
     1261 cgttcacggt gacgatgtgg acgccctcgc ccgtgagcgc gttgaggtag gcaggaaggg
     1321 tcgccaccag cgtcttgccc tcgccggtgc gcatctcggc gatcatgccc ttgtgcagca
     1381 cgatgccgcc gatcagctgc acgtcgaagt ggcgcatctt gagcgtgcgc cgcgccgcct
     1441 cacgcacgac cgcgaacgcc tccgggagca gctcctcgag gctcacgccc tctttcaggc
     1501 gagcccgaaa ctcatcggtc ttgccgcgca gcgcctcgtc gctcaaggac tcgatccgcg
     1561 gctcgagctc gttggcggcc cgcacgtagc gcgcgtagcc gcgcagcagc cgctcgttgc
     1621 ggctgccgaa gatgcgcgtg aggaagtcga gcaagggcta tccgtgaaat gagagaaaat
     1681 atcttgcccg acaagcactt accgtctgca cgggctcgac gaagcggcgt attgtacgca
     1741 atagacgcga ccaccgccca attggttcgc gggcggcgga attcaaggtg ggaagggatc
     1801 agcggccgat gaaggacagc gggtcgacct ggcggccgtt gtgcagaact tcgaagtgca
     1861 cgtgcggtcc cgtcgagtgg ccggtcgagc ccatgagggc gacggcctcg ccgcgcttga
     1921 cggtctgtcc tacggagacg agggtgcgcg agttgtgcgc gtaacgggtc gcgtagccgt
     1981 cgccgtgatt gatctcgatg agcttgccgt agcctgagcg ttccccggcc caggtgacga
     2041 ctccggccgc cacggcgacc acgtcggagc cgagggcgcc ggcgaagtcc acgcccttgt
     2101 ggaaggcctc gcgcccgtcg aagggatcag ggcgctcgcc gaaataagag gagatgtagc
     2161 cggtcgcgac cgggcgaccc tcggggtgaa tctcctcttt cagctcgcgc gtcaggatca
     2221 cgttctcgag cgccgccagc tgcgattcgc gcaggtccgc acgctgctcg agccgggcga
     2281 gcatggccga gagatccggg atctgggcgc tcacgccctc gccttccgga ccaccgctcg
     2341 gcggactgcg atcgaagtcg aactcgcggc tgtcgatgtc cgccatctcc gtgaggcgct
     2401 tgccgagcgc gttcaggcgg atgatgtggg cgttcacctc gccgatgcgc atcgccatcg
     2461 cgtcgatccg cagctgcagc tgctgcttga ggccggcgat ctcctgcttc tgctgggcga
     2521 gaaccgcggc gaaacgcgtc gtctcggcga gcgcgatctc gcggtgcacg cccttgccga
     2581 gctgccatcc gagcgtgaac acccccgcga cgccgagcac cgtcagcccg aggaggccgc
     2641 cgagcgtccg gggccgggca agattgaact gccgggccct gccgtcgcgc cgcgatagaa
     2701 gaatgacgtt catgccaaag gttccgggta ccttatgtca cccacgggta cagagaggca
     2761 ccccagactc gagcgtgtac cgcccttgtt ctcgttcaga caaatctctg tacgatgctg
     2821 gcaaccccgc cgtcatggct cctcgcttta gaccggtggc tttgcgtccc cgcctttcag
     2881 cgggtttgcc gatacagctg cctggccaat atgcccaaga agacccgacg aaacaagctc
     2941 ggcagttccc gaatgaggaa tcgcaccgtc gccggttcgc gacaaccgga ttctgtaaag
     3001 gacctgcttg cacggcgtgc gccatccctg atgcgcgtca caacacaggc ggcgcgcgcg
     3061 cgtttctgga gcgattggct gtcgcagcac ctgcccgccg aggtcggcac gcgcgtctcg
     3121 ggcgtcgtcg agcgcgacgg caaactggtc attttcgccg agtccgccgc ctggtccgcg
     3181 cggctgcgct acctcgtcca ggagctcgag ccgcagatcc tcgctgccgc ccgcggcctc
     3241 accgccgttg cggtgcgggt tcgtccgcgc gactaactcc gccgcgagcc cggcaacgcc
     3301 ggcgcctcga tataggaaat cggcgcgtcc ttgtacgact ggaacacgac ctcctcccag
     3361 gtggaggcgt cggccagcag cacgcgcagc agccgattgt tgagcgcgtg gccggacttg
     3421 tgaccggaga attcgccgat caggctgtgt ccgagcaggt acaggtcgcc gatggcgtcg
     3481 aggatcttgt gcttgacgaa ctcgtcctcg taacgcagcc cgtcctcgtt cagcacgcgg
     3541 aaatcgtcga gcacgatcgc gttgtcgaga ttgccgccga gggcgagatt gcgcgcccgc
     3601 agtgtctcga ggtcgcgcat gaagccaaag gtgcgcgcgc ggctcacctc cttgaggaag
     3661 gaggtggtgg agaagtccat ggaggcgcgc tgcgagcggc gcttgaagat cgggtggttg
     3721 aactcgatct cgaagttcac cttgaagcca tcgaagggat cgaactgcgc ccacttgtcg
     3781 ccgtcctcga cccgcagacg cttcttgacg cggatgaagc gcttcgggcg gcgctgctcc
     3841 tcgatgccgg ccgactgcag cagaaacacg aagggaccgg cgctgccgtc catgatcggc
     3901 acttccgccg ccgagagatc gacgacggcg ttatcgatgc cgaggcctgc gaacgccgag
     3961 agcaggtgct ccaccgtcga gacgcgcgac tcaccctgtc cgagcgtggt acccaacatg
     4021 gtgtcgccga cgttgtcggc gctcgccctg atgtctaccg gctgggccag gtcggtgcgt
     4081 cggaaggtga tgccctcgtc cgggtccgcc ggcttgaggg tc
//
LOCUS       DATLKL010000560         2827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_6443, whole genome shotgun sequence.
ACCESSION   DATLKL010000560 DATLKL010000000
VERSION     DATLKL010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6443"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VM713_07520"
     CDS             <1..473
                     /locus_tag="VM713_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012410.4"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HVH84146.1"
                     /translation="RFKDAARGDLDLGPLASTADKARHDAELKESKGLLKRIKDALGE
                     RVAEVRISDRLKDSPALLVLGEHELGDRLRRILSAAGQKVPEVKPVLEVNVTHPLVKY
                     LDGLADPSGFSELAQLLYEQAQLAEGAALANPPDFVLRLNRLLVRLAARAGQGS"
     gene            599..1276
                     /locus_tag="VM713_07525"
     CDS             599..1276
                     /locus_tag="VM713_07525"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVH84147.1"
                     /translation="MVLKPPRAERLAIEGPAGALQVLVETPVIEHGEPLTAPAFAVVC
                     HPHPLFGGTMDNKVVYTVARAFEQRGAPAIRFNFRGVAASAGTYDEGRGESDDALAVI
                     AWGRQRFPDTPLWLAGFSFGGAVALRIAELAAPQRLVLVAPGITRLERADFAAARAAE
                     PTCPWLIVQGAADDVVPAPAVLAWAAERRPLPGVAPPEVRLVPDAGHFFHGRINELRE
                     AVLAFPG"
     gene            complement(1357..1671)
                     /locus_tag="VM713_07530"
     CDS             complement(1357..1671)
                     /locus_tag="VM713_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVH84148.1"
                     /translation="MAKKGGAPTKSEVLTQISKETGLSRKQVGSVFDSLSGVIKKSLR
                     GGGLFTLPGLLKMKVVKKPATKAREGINPFTGEKMMFKAKPASKKVRCLPLKSLKAMV
                     NS"
     gene            complement(1965..2492)
                     /locus_tag="VM713_07535"
     CDS             complement(1965..2492)
                     /locus_tag="VM713_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HVH84149.1"
                     /translation="MSAVIYIVETLLSLALFVVLARLLLQLTRADFRNPICQAIVQLT
                     NPLIVPLRRVLPPLGKVDTASVVAVLLVAGVEVAVLAALRGGGLPDWLTWLDALALEI
                     AHTLLWTYCYAILVYWLLSFIAPGGYSPLQSVLSSLCEPVLRPFRRIIPQVAGLDLSY
                     LWATIAIQALLILIR"
     gene            complement(2503..>2827)
                     /locus_tag="VM713_07540"
     CDS             complement(2503..>2827)
                     /locus_tag="VM713_07540"
                     /inference="COORDINATES: protein motif:HMM:NF026098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HVH84150.1"
                     /translation="VTALSGSGPAYFFLLAELMADAATGLGLPSAVAKRLAAAMLYGA
                     GLMAATGDADLARLRAEVTSKGGTTEAALGVFASADLEATVRDALAAAARRARELGAL
                     AEGHS"
BASE COUNT          435 a          962 c          987 g          443 t
ORIGIN      
        1 agcgcttcaa ggatgccgcg cgcggcgacc tcgatctcgg gccgctcgcc agcacggccg
       61 acaaggcgcg gcacgatgcc gagctgaagg agagcaaggg gctgctcaag cgcatcaagg
      121 acgcactcgg cgagcgcgtc gctgaagtgc ggatcagcga ccgcttgaag gactctccgg
      181 cgttgctggt gctgggcgag catgagctcg gcgaccgcct gcggcgcata ttgagcgccg
      241 ccggccagaa ggtccccgag gtgaagcccg tcctcgaggt caacgtgacc catccgctgg
      301 tgaagtatct cgatgggctc gctgacccca gcggcttcag cgagctcgcg cagctcctct
      361 acgagcaggc gcagcttgcc gaaggtgcgg cgctcgccaa tccgcccgac ttcgtgctgc
      421 gcctgaaccg gctgctggtg cgtctcgccg cccgcgcggg gcaaggcagc tgagcgcagg
      481 cacacggacg tgcccgccgc cggaggcggc gagcggcgcg ccgaaatcgc gcttttggcg
      541 cgccgccgcg agcatcgtga caggatgtcc gatgcgagca attcactaga atgcgcgcat
      601 ggtgctgaaa ccgccgcgcg ccgaacggct cgcgatcgag ggcccggccg gcgcgctgca
      661 ggtgctggtc gagacgccgg tgatcgagca cggcgagccg ctcactgcgc cggcctttgc
      721 cgtcgtgtgc catccgcatc cgctcttcgg cggcaccatg gacaacaagg tcgtctacac
      781 ggtggcgcgc gccttcgagc agcggggagc gccggcgatc cgcttcaact tccgcggcgt
      841 cgcggccagc gccgggacct acgatgaggg gcgcggtgag agcgacgatg cgctcgcggt
      901 gatcgcctgg ggccggcagc gctttcccga cacgccgctt tggctcgccg gattctcctt
      961 cggcggggca gtggcgctgc gcatcgcgga gctggccgcg cctcagcgcc tggtgctggt
     1021 cgcgcccggc atcacgcggc tcgagcgcgc cgacttcgct gccgcgcggg ccgccgagcc
     1081 cacctgtcca tggctcatcg tgcagggagc ggcggacgat gtggtgccgg ccccggcggt
     1141 gctcgcctgg gcggccgagc ggcggccgct ccccggagtg gctcctcccg aggtgcggct
     1201 ggtgcccgac gcggggcact tcttccacgg acgcatcaac gagctgcgcg aggctgtgct
     1261 cgccttcccg ggctgaggaa aagcaaaagc ccgggcggag ctgtgcttcc gcccgggcct
     1321 caggtccgcc tgttgcgcgg cgatttaccc gtcgctttac gagttgacca tcgccttcag
     1381 gctcttcagc ggcaggcagc gcaccttctt gctcgccggc ttggccttga acatcatctt
     1441 ctcgccggtg aaggggttga taccctcgcg ggccttggtg gccggcttct tcacgacctt
     1501 catcttcagc agtccgggca gggtgaacag tccgccgcca cgcaggctct tcttgatcac
     1561 gccgctgagc gaatcgaaga cgcttccgac ctgcttgcgg gacagccccg tctccttgga
     1621 gatctgtgta aggacttccg atttcgtcgg tgcgccaccc tttttcgcca ttttgcggta
     1681 ctcctcgagg attttggttg gttaagacgc gcgccattca gccccgcgcg cagagaatcg
     1741 gcgcggaaaa tagcacatgc gcgcgctttg gcaagcggtt tttctctttc gctgttgcgg
     1801 aaatgccttt tttcgtaggc tttgcgctgc ggggcccgtc gccacccgtg ccgccgggcg
     1861 cgcggcgcgc ggcacacgaa gcgcttgaca cgcgcgcgcg atgttgcgac ttacgatgcg
     1921 aagtgcgcgc taagcgcgtg ttccgccaat gcggatgcct gacttcagcg gatgagaatg
     1981 aggagcgctt gtatagcgat ggtcgcccag aggtacgaga gatcgaggcc cgcgacctgt
     2041 ggaatgatgc gccggaaggg ccgcagcacc ggctcgcaca gcgagctgag caccgactgc
     2101 agcggagagt aaccgcccgg tgcaatgaag ctcagcagcc agtagacgag gatcgcgtag
     2161 cagtaagtcc agagcagcgt gtgcgcgatc tcgagcgcaa gcgcatcgag ccaggtgagc
     2221 cagtcgggca gtcccccgcc gcgcagcgcc gcgagcaccg ccacttccac gccggcgacc
     2281 agcagcaccg ccaccaccga ggcggtatcc accttgccga gcggtggcag cacacgccgc
     2341 agcggcacga tcagcgggtt ggtgagctgt acgatcgcct ggcagatcgg gttgcggaag
     2401 tccgcccgcg tcagctgcag cagcagccgc gccagcacca cgaacagcgc gagcgacagc
     2461 agcgtctcga cgatgtagat gacggcgctc attgggcggt actcacgagt ggccttcggc
     2521 gagggcgccg agctcgcgcg cgcggcgcgc agcggcggcg agcgcgtccc ggaccgtcgc
     2581 ctcgaggtcg gcggaggcga acaccccgag cgccgcctcc gtggtgccgc ccttggaggt
     2641 cacctcggcc cgcagccgtg ccaggtccgc atcgcccgtc gcggccatga gacctgcccc
     2701 gtagagcatc gcggcggcga gccgcttcgc cacggctgaa ggcagtccga ggccggtcgc
     2761 cgcgtcggcc atgagctcgg cgagcaggaa gaagtacgcc ggcccgctgc ccgagagcgc
     2821 ggtgacg
//
LOCUS       DATLKL010000561         1640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_97078, whole genome shotgun sequence.
ACCESSION   DATLKL010000561 DATLKL010000000
VERSION     DATLKL010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97078"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1640
                     /locus_tag="VM713_07545"
     CDS             <1..>1640
                     /locus_tag="VM713_07545"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVH84151.1"
                     /translation="RQIIELQPKVNYETLAPAHQAIKAVRKAAAALSLDPAHGVKVRL
                     TGGAAMDEDELKSVEHGVGLASVISLVLVSVLVFLGLRSPRLVFATLATLLLSLMWTA
                     AFAALAIGYLNLISVAFAVLFIGLAVDFSIQFGLRYKESVDHGADLAEALRNAAANTG
                     IAVSLAALCAAIGFFAFVPTSYVGLSQLGVIAGVGMFIGLFATLTLLPAILSLIPLKP
                     SRRVAEDRNGRAVGSFLERHARAVCWGALALGLVSLAAFPYARFDIDPINLKDPRSES
                     VQAYLDMTKDSSTSPYAINIMAPSLEGADTLAKRLDKLPEVDSTVTLSSFVPQDQDAK
                     LALVDQMATFLTPVIDGGKQKSPPSDPERRQAARDLRQKLAELAAWPKAGPVAEPARR
                     LAELLARYDAGPGRDPRAYGALEDALVANLPGRLQGLSEAMKAGPVSLDNLPANLKAR
                     YVTPDGRARIEVFPEERLSNEDALRRFVGAVRQIAPNATDTPVLLFEGGKVVVGAFEQ
                     AGVIALVAIGILLVIVLRSFWDSLLVVLPLALAAVLTV"
BASE COUNT          252 a          542 c          544 g          302 t
ORIGIN      
        1 ccgtcaaatc atcgagctcc agcccaaggt caactacgag acgcttgcgc cggcccatca
       61 ggccatcaag gcggtgcgga aggcggcagc ggcgctctcg ctcgatccgg cgcacggcgt
      121 gaaggtgcgg cttaccggcg gcgcggccat ggacgaggat gagctcaaga gcgtcgaaca
      181 cggcgtcggg ctcgcgagcg tcatctcgct cgtcttggtc agcgtgcttg tctttctggg
      241 cctccgctcg ccgcggctag tattcgcgac gctcgcgact ctgctcctca gcctcatgtg
      301 gaccgccgct ttcgccgcac ttgcgatcgg atatctgaac ctgatctcgg tcgccttcgc
      361 cgtgctcttc attggccttg cggtggattt cagcattcag ttcggcctgc gctacaagga
      421 aagcgtcgat catggtgccg atctcgcgga ggcgctgcga aacgcggctg ccaataccgg
      481 gatcgccgtg agcctggccg cactctgtgc ggcgatcggc ttcttcgcct ttgtgccgac
      541 cagctatgtc ggcctgtcgc aactcggcgt catcgccgga gtgggcatgt tcattggtct
      601 gttcgccacc ttgacgctgc tcccggcgat attgagcttg attccgctca agccctcgcg
      661 gcgggtcgcc gaggatcgaa acgggcgcgc ggtaggcagc tttctcgagc gccatgcccg
      721 cgcggtctgc tggggcgcgc tcgcgcttgg attggtgtcc ctcgcggcct tcccctatgc
      781 ccgtttcgat atcgacccga tcaacctcaa ggatccccgc agcgaatcgg tccaggccta
      841 cctggacatg accaaggact cgagcacatc gccctacgcg atcaacatca tggcgccgag
      901 cctcgagggt gccgacacgc tcgccaagcg actcgacaag ctccccgagg tcgacagcac
      961 ggtgacgctg agctcgttcg tgccccagga ccaggacgca aagcttgcgc tcgtcgacca
     1021 gatggccacc ttcctgacgc cggtaatcga tggcggcaag cagaagtcgc cgccgagcga
     1081 tcccgaacgg cgccaagccg cgagggacct gcgccagaag ctcgcggaac tcgccgcatg
     1141 gccgaaggcg ggaccggtcg cggagccggc gcgccgattg gccgagttgc tcgcgcgcta
     1201 cgacgccggg ccggggcgcg atccccgggc gtatggggcg ctcgaggatg cgctagtcgc
     1261 caatttgccg ggccgtcttc agggcctgtc cgaagccatg aaggccggtc cggtcagcct
     1321 cgacaacctg cccgcaaacc tcaaggcgcg ctatgtgacg ccggacgggc gggcgcgcat
     1381 cgaggtgttt ccggaggagc ggctgagcaa tgaggacgcg ctccgtcgtt tcgttggcgc
     1441 ggtacgtcag atcgcgccga atgcgacgga cacgccggtg ctcctgttcg agggcggcaa
     1501 ggtggtggtc ggcgccttcg agcaagcagg ggtcatcgcc ctcgtggcga tcggcatcct
     1561 gctggtgatc gtgctgcgca gcttctggga ttcgctgctc gtggtgctac cgctggcgct
     1621 tgcagccgtg cttacggtgg
//
LOCUS       DATLKL010000562         1641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_108217, whole genome shotgun sequence.
ACCESSION   DATLKL010000562 DATLKL010000000
VERSION     DATLKL010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108217"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VM713_07550"
     CDS             complement(<1..603)
                     /locus_tag="VM713_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVH84152.1"
                     /translation="MNDRHLPPERLAARSAEIGGGLNIRRALPNRQRRTVGAWCFLDH
                     AGPMEYSQDGGMHVGPHPHIGLQTFTWMIEGEVVHRDSLGNEQVITPGQVNLMTAGAG
                     IAHAEDSAPGRAGRLHAAQLWIALPEGERRRPPAFRNYPRLPLLESGGFSVRVLAGSA
                     FGQTSPAEVYSPLVGLDVSATGPAALTLPLTAPFEHAALAL"
     gene            complement(600..1016)
                     /locus_tag="VM713_07555"
     CDS             complement(600..1016)
                     /locus_tag="VM713_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVH84153.1"
                     /translation="MSNPRAVTAISGATPYVVTLTDREGHHWLADEPLGAGGGNRGPA
                     PTELLLSSLGACTAMTVQMYATRHSWPLMGVEVGLEFNPEGAPAGGGSHIRRKITLTG
                     DLTHEQRERLLDVAEKCPVHRILTGKIHIETSLTRA"
     gene            complement(1027..1341)
                     /locus_tag="VM713_07560"
     CDS             complement(1027..1341)
                     /locus_tag="VM713_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84154.1"
                     /translation="MPFVIRWIDEAGFVSAPTAAYGVVLACAALSYTNLQAQIIALNG
                     RESRLAVATGSDFKGKLSVALYLAAIVLAFVRPWFAILLYVTVALIWLVPDTRIESLV
                     RK"
     gene            complement(1375..>1641)
                     /locus_tag="VM713_07565"
     CDS             complement(1375..>1641)
                     /locus_tag="VM713_07565"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HVH84155.1"
                     /translation="RDDAAIGCTYDPPMEPERLEAFTDGVVAIIITIMVLEIRVPHGT
                     DLAALQADIPVLVAYVLSYVNVGIFWNNHHHMLHVTERVNGKIL"
BASE COUNT          260 a          513 c          605 g          263 t
ORIGIN      
        1 caaggcgagc gccgcgtgct cgaacggtgc cgtgagtggc agggtgagcg cggccgggcc
       61 ggtagcgctc acgtcgaggc cgacgagcgg agagtaaacc tcggcgggag aggtctgccc
      121 gaaggcgctc ccagcgagta cgcgcacgct gaaaccaccg gactcgagga gcggcaggcg
      181 cggatagttg cggaaggcgg gcggacggcg ccgctcgccc tcgggcaggg cgatccagag
      241 ctgcgcggcg tgcagccgcc ccgcgcgacc gggcgcggag tcctcggcgt gcgcgattcc
      301 ggctcccgcc gtcatgaggt tcacctggcc cggcgtgatg acctgctcgt tgccgagact
      361 gtcgcggtga accacctcgc cctcgatcat ccaggtgaag gtctgcagcc cgatgtgcgg
      421 atgcggcccg acgtgcatgc cgccgtcctg ggaatactcc ataggccccg cgtgatcgag
      481 gaagcaccac gcgcccacgg tgcgccgttg gcgattcggc agcgcgcgac ggatgttgag
      541 cccgccgccg atctcggcgg agcgtgccgc gaggcgctcg ggcgggaggt gccggtcgtt
      601 catgcgcgcg tgagggaggt ctcgatgtgt atcttgccgg tcagaatccg gtggaccggg
      661 catttctcgg cgacgtcgag cagccgctcg cgttgctcgt gggtcaagtc tccggtgagc
      721 gtgatcttgc gccggatgtg gctgccgcct cctgccggtg caccctcggg gttgaactcg
      781 agcccgacct cgacgcccat gagcggccag ctatggcgcg tagcatacat ttgcacggtc
      841 atcgccgtgc acgccccgag gctcgagagc aacagctccg taggcgcggg gccgcggttg
      901 ccgccgcccg ccccgagcgg ttcgtcggcc agccaatgat gaccctcgcg atccgtgagg
      961 gtgacgacgt agggagtagc gccgctgatc gcggtcacgg cccggggatt gctcatttgg
     1021 gtgttctcat ttcctgacca gcgattcgat gcgcgtgtcg ggcaccagcc agatcagcgc
     1081 cacggtcacg tacaggagga tcgcgaacca ggggcgcacg aatgccagga cgatggcggc
     1141 gaggtagagc gcgacggaga gctttccctt gaagtcgctg cccgttgcga cggcgaggcg
     1201 cgactcgcgg ccgttgagcg cgatgatctg tgcctgcagg ttggtgtagc tgagcgcggc
     1261 gcaggcgagc accacaccgt aggcggcggt cggtgcggac acgaagcccg cctcgtcgat
     1321 ccagcggatc acgaacggca ccagcgacag ccagaagagc agccccaggt tggctcagag
     1381 gatcttgccg ttgacccgct cggtgacgtg cagcatgtgg tggtggttgt tccagaagat
     1441 gccgacgttg acgtagctga ggacgtacgc gacgagtacc gggatgtcgg cctgcagcgc
     1501 ggcgagatcg gtgccgtgcg ggacgcgtat ctccagcacc atgatggtga tgatgatggc
     1561 gacgacgcca tcggtgaagg cttcgaggcg ctcgggctcc atgggtggat cgtacgtgca
     1621 tccgatcgcc gcgtcatcgc g
//
LOCUS       DATLKL010000563         1564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_40574, whole genome shotgun sequence.
ACCESSION   DATLKL010000563 DATLKL010000000
VERSION     DATLKL010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40574"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VM713_07570"
     CDS             <1..522
                     /locus_tag="VM713_07570"
                     /inference="COORDINATES: protein motif:HMM:NF023096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HVH84156.1"
                     /translation="GACWTLVSFVLVVIGLALFSLPLFLRAKPADIELAPKDVAVLAV
                     LTAMLIVLHEGIHGIVMRRFGARPFFGVSMAAKIVPALYTTAPGYRFSRWQYLAVSLA
                     PAIVISLVGFAVSFSSAGPFLFVPLALHLGGCAGDAAATRQVLRQPRGTIYEDLRDGI
                     RFHRPSPGGVSIS"
     gene            522..>1564
                     /gene="glyA"
                     /locus_tag="VM713_07575"
     CDS             522..>1564
                     /gene="glyA"
                     /locus_tag="VM713_07575"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HVH84157.1"
                     /translation="MATNITTMTFRALDAVDPEVANLIRHEFKRQSETLELIASENLT
                     SPAILEALGTLLTNKYAEGYPGRRYYGGTGEVIDKIETLAIERAKQLFGADHVNVQPH
                     SGSQANAAVYMAVCQPGDRVMGMDLSAGGHLTHGSPANFSGKLYEIHSYGVDRETERI
                     DYDVVQKEAEQVRPKMLLAGFSAYSRIVDWKRMREIADSVGATLFVDMAHVAGLVAGG
                     VYPSPIPHAAITSTTTQKTLGGAWGAIILCTNDWQKAIDSAVFPGTQGGPLMHAIAAK
                     AVCFGEALKPDFSVYARQVVANAQAMAGQLTNRGLRLVSGGTDNHLMLIDLRPQKRTG
                     KQVQDIADTVGITL"
BASE COUNT          271 a          506 c          514 g          273 t
ORIGIN      
        1 ggcgcgtgct ggacgttggt gagcttcgtc cttgtggtaa tcgggctcgc gctcttttcc
       61 ctcccgctgt tcctgagagc caagcccgct gacatcgagc tcgccccaaa ggatgtggcg
      121 gtcctggcgg tcttgacggc catgctgatc gtgctgcacg aaggcatcca cggcatcgtg
      181 atgagaaggt tcggagcgag accattcttc ggcgtctcga tggcggcgaa gatcgtgccc
      241 gcgctgtaca cgaccgctcc gggctatcgc ttcagccgct ggcagtatct ggcggtgtca
      301 cttgctcccg cgatagtcat ctcgctggtg ggctttgcgg tttccttcag ttcggccggc
      361 cccttcctct tcgtgccgct ggcgcttcac ctcggcggct gcgccggcga tgcggccgcc
      421 acgaggcagg tgttgcggca gccgcgcggc accatctacg aggaccttcg cgacggcatc
      481 cgcttccacc ggccatcccc gggcggggtt agcatcagct gatggcgacc aacatcacga
      541 cgatgacgtt tcgcgccctg gatgcggtcg atcccgaagt tgccaacctg atccgccacg
      601 agttcaagcg tcagtccgag acgctcgagc tcatcgcatc ggaaaacctg acgagcccgg
      661 ccatcctcga ggcgctgggc acgctgctca cgaacaagta cgcagagggc tatccagggc
      721 ggcgctacta cggcggcacc ggggaggtga tcgacaagat cgagacgctc gccatcgaac
      781 gggcgaagca gctcttcggc gcggatcacg tcaacgtgca gccgcactcg ggatcccagg
      841 caaacgccgc ggtctacatg gcggtgtgcc agcccggaga tcgcgtgatg ggcatggacc
      901 tgagcgccgg cggccatctg acgcacggta gcccggctaa cttctcaggg aagctctacg
      961 agatccactc gtacggcgtc gaccgcgaga ccgagcgaat cgactacgac gtcgtgcaga
     1021 aggaagcgga acaggtgcgg ccgaagatgc tgctcgccgg cttctccgcg tattcgcgaa
     1081 tcgtcgactg gaagcgcatg cgcgagatcg cggactcggt cggcgcgacg ctattcgtgg
     1141 acatggccca cgtggcgggt cttgtcgcgg gcggcgtgta tccctctccg ataccgcatg
     1201 cggccatcac gtcgacgacc acgcagaaaa cgctgggcgg cgcctggggc gcgatcatcc
     1261 tctgcaccaa cgactggcag aaggcgatcg actccgccgt ctttcccggc acgcagggcg
     1321 gaccgctgat gcatgcaatc gcggcgaagg cggtgtgctt tggggaggcg ctcaagcccg
     1381 atttcagcgt gtacgcgcgg caggtcgtgg ccaacgccca ggcgatggcc ggtcagctga
     1441 cgaaccgcgg tctgcggctt gtcagcggcg gtacagacaa tcacctgatg ctcatcgacc
     1501 tgcggccgca gaaacggacg ggcaagcagg tccaggacat cgccgacacg gttggcatca
     1561 cgct
//
LOCUS       DATLKL010000564         2287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_40582, whole genome shotgun sequence.
ACCESSION   DATLKL010000564 DATLKL010000000
VERSION     DATLKL010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40582"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VM713_07580"
     CDS             complement(<1..529)
                     /locus_tag="VM713_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84158.1"
                     /translation="MGRWLLLGCLWLAAPLATAAETARETYAGALLEEFARGSPQLLA
                     AAISATPPKGTAAVVVAATDRSLAGTPADPRDIAASKDQSNTSAPSASGERFEIRLPL
                     HDVSGDTVGALRLSYSYHAGDDRAALERAAATLRDRLARRISHAANLFDPYPYEPEAP
                     KHTYAQKLVDEFLERY"
     gene            complement(636..1667)
                     /locus_tag="VM713_07585"
     CDS             complement(636..1667)
                     /locus_tag="VM713_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HVH84159.1"
                     /translation="MRCLTLLGAALLAMGALQIRQSAAATAGSYRMIAQYPIGGNDTG
                     YDYLRVDAAARRVFVAHGNRVEVLDVDTGKKVGEIGGMHGVHGIELIPELGKGYTSNG
                     LDRTVTVFDLATLQVRKLIKYTGVKPDAIQYDPDTRRLFVVNGGASGDVTVIDPSLDV
                     IVAALDLGGSKLEQIGFDARGRAFVNDEGKSVVHVFDTHALQPLAKWSLGGCEEPTGM
                     AVDRDHRRVFAACGNQKLAVLDSDNGRVVATPAIGADPDGALFDPRTQRIFTSNREGT
                     LSVLRELTPDRYETLQTLVTLPGARTLAMDERSGRIFLPTARFGPAPAGGGAAPIIAG
                     TFELLVVGP"
     gene            1725..>2287
                     /locus_tag="VM713_07590"
     CDS             1725..>2287
                     /locus_tag="VM713_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH84160.1"
                     /translation="MKILVVEDERKLSALLRQGLSEAGYGVGVAATCAAARDLLSDTS
                     FDAIVLDLGLPDGDGLELLRQWRRNGFNEPVIILSARDAVHDRVKGLDFGADDYLPKP
                     FSLQELLARLRSLLRRHSGVKDTVLEHRGLRLDLLARTLHLDDQPVELTNREFALLEL
                     FMQNPGRGPLRGRAGGRPARVAGVRAGR"
BASE COUNT          355 a          774 c          779 g          379 t
ORIGIN      
        1 ggtagcgctc cagaaactcg tccacgagct tctgcgcata ggtgtgtttg ggcgcttccg
       61 gctcgtacgg gtacgggtcg aacaggtttg cggcgtggga aatccgccgt gccagccggt
      121 cacgcagagt ggcggccgcc cgctcgagcg ccgcccgatc atcgcccgcg tggtaggaat
      181 aactcagacg cagcgcgccg accgtgtcgc cggagacatc gtgcagcggc agccggatct
      241 cgaagcgctc gccactcgcg ctcggggccg aggtgttgga ttgatctttg ctcgcggcga
      301 tgtcacgcgg atcggcggga gtgcccgcga gcgagcggtc ggtggcggcc accaccaccg
      361 ccgccgtgcc cttgggcggg gtggcgctga tcgccgccgc cagcagttgc gggctgcccc
      421 gggcgaattc ctcgagcagc gcgcctgcat aggtctcccg cgccgtctcc gcggccgtcg
      481 ccagcggagc cgccagccaa agacacccga gtagcagcca acgccccatc cgactctccc
      541 ccgccgcgcg ctatgcacca acttgcagcg tggcgacttt gtagcgcacg actctgaagc
      601 cagcctgaaa tcctgcaccg tcggggtgcg tgcgctcagg ggccgaccac gagcagctcg
      661 aaagtccccg cgattatcgg agccgctccg ccacccgcgg gtgcggggcc gaagcgcgcc
      721 gtgggaagaa agatccggcc actgcgctca tccatcgcca gcgtgcgggc gccgggcagc
      781 gtgaccaggg tctgcagcgt ctcatagcga tcaggcgtca gctcccgcag gacgctcagc
      841 gtgccctcgc ggttcgaggt gaagattcgc tgtgtgcgcg ggtcgaacag cgcgccgtcc
      901 gggtcagcgc cgatggccgg agtcgccacg acccggccgt tgtcgctgtc gagcaccgcg
      961 agcttctggt tgccgcaggc agcaaacaca cggcggtggt cccggtccac cgccataccg
     1021 gtcggctcct cgcagccgcc gagcgaccac ttcgcgagcg gttgcagggc atgcgtgtcg
     1081 aacacgtgga cgacgctctt gccctcatcg ttgacgaagg cacggccgcg cgcatcgaag
     1141 ccgatctgct cgagcttgct gccgccgaga tccagcgctg cgacgatcac atcgaggctc
     1201 ggatcgatga cggtcacatc gccgctcgcg ccaccgttga ccacgaacag gcggcgcgtg
     1261 tcggggtcgt actggatggc atcgggcttc actcccgtat atttgatgag cttgcgtacc
     1321 tggagggtcg ccagatcgaa caccgtcacg gtccggtcga ggccgttgct ggtgtagccc
     1381 ttgccgagct ccggaatcag ctcgatgcca tggacgccat gcatgccgcc gatctcgcca
     1441 actttcttgc cggtgtcgac gtccaggact tccacgcggt tgccgtgcgc cacgaacacc
     1501 cggcgcgccg ctgcatcgac ccgcagatag tcgtagcccg tgtcgttgcc accgattggg
     1561 tattgggcaa tcatgcggta gctgcccgcc gttgcggcgg cggactgcct gatttgcagc
     1621 gcgcccatcg cgaggagcgc cgccccgagc agcgtgaggc atcgcatgac cggattcctc
     1681 ctgattgaac acggcgcatt ctgacgtcgc aacctgaagg caaagtgaaa atcctggtcg
     1741 tcgaggacga gcgcaagctg tcggccctgc tgagacaggg actctccgag gccggatacg
     1801 gggtgggcgt ggcggccacc tgtgctgcgg cgcgcgacct gctcagcgac acgagcttcg
     1861 acgccatcgt gttggacctc gggcttcccg atggcgacgg tctcgagctg ctgcgccagt
     1921 ggcgccgcaa cggctttaac gagccggtga tcatcctgag tgcgcgcgac gcggtgcacg
     1981 atcgcgtcaa gggtctggac ttcggcgccg atgactatct gccgaagccc ttcagcctgc
     2041 aggagctgct ggcccgcctg cgttcgctgc tgcgcagaca ttcgggcgtc aaggacacgg
     2101 tactcgagca tcggggactg cggctggatc tgctggcgcg cacgctgcac ctcgatgacc
     2161 agccggtcga gctgaccaac cgggagttcg cgctgctcga gcttttcatg cagaacccgg
     2221 ggcgcggtcc gctccggggc cgcgcgggcg gccggcctgc gagggtcgca ggtgtacggg
     2281 caggtcg
//
LOCUS       DATLKL010000565         1592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_74585, whole genome shotgun sequence.
ACCESSION   DATLKL010000565 DATLKL010000000
VERSION     DATLKL010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74585"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VM713_07595"
     CDS             complement(<1..476)
                     /locus_tag="VM713_07595"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVH84161.1"
                     /translation="MTRRIFVTTALPYANGPFHIGHIMEYIQADIWVRFQRLQGHQVH
                     FVCADDAHGAPIMLKAEAEGVTPQQLVARIAAGRPRYLAGFHLSFDHWHSTESPENTR
                     LSQDVYARLKAAGLIYKKDIEQFFDPLKGMFLPDRYIKGECPNCHSPDQFGDACEV"
     gene            complement(518..>1592)
                     /locus_tag="VM713_07600"
     CDS             complement(518..>1592)
                     /locus_tag="VM713_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HVH84162.1"
                     /translation="EPDVASQIDPFLHEILEKRGSDLHFIAGDSPRIRVYGDLTPLRP
                     DKLNADYVKTALYEIMPKTAVDRFEKKDGADFAYTLEGKGRFRVNVMRQLNGMGGVFR
                     AIPSKALTLDELKLPEAVRGLCRANSGLILVTGKTGSGKSTTLAAMIDDINRRVNGHI
                     LTVEDPIEFVHERQNCLISQREIGVHSPSFAAAVHSALREDPDVVLVGELRDLETMSI
                     AVTAAEMGILVMGTLHTNGAAATVDRMVNVFPSDKQSHVRTMLSTSLRGVVSQQLLQR
                     ADRQGRVAALEILVNTPAAANLIRQGKLDQLENTMQAGGQFGMRTMDTAIQQLLDSRQ
                     ITGKEAYKKAINKAKFEEFREAG"
BASE COUNT          237 a          506 c          547 g          302 t
ORIGIN      
        1 cacacctcgc aggcgtcgcc gaactgatcg ggcgagtggc agttgggaca ctcgcccttg
       61 atgtagcggt cgggcaggaa catgcccttg agcggatcga agaactgctc gatgtccttc
      121 ttatatatga gcccggcggc cttgaggcgc gcgtagacat cctgcgagag gcgggtgttc
      181 tcgggtgatt cggtcgagtg ccagtggtcg aagctcaggt gaaagcccgc gaggtagcgt
      241 ggccgtcccg cggcgatgcg cgcgaccagt tgctgcggcg tcacgccctc ggcctccgcc
      301 ttcagcatga tcggcgcgcc gtgcgcgtcg tcggcgcaga cgaaatgcac ctggtgtccc
      361 tgcaggcgct ggaagcgcac ccagatatcc gcctggatgt actccatgat gtgcccgatg
      421 tggaacgggc cgttggcgta ggggagcgcc gtggtgacga agatccgccg ggtcatccag
      481 ctagtttacc gtcccggcgc ggcgcgtgcc gtcggcttca gccggcttcg cggaactcct
      541 cgaacttcgc cttgttgatc gccttcttgt aggcctcttt gccggtgatc tgccgcgaat
      601 cgagcaactg ttggatcgcg gtgtccatgg tgcgcatgcc gaactggccg ccggcctgca
      661 tggtgttctc gagctggtcg agcttgccct ggcggatgag gttggcggcg gccggggtgt
      721 tgaccaggat ctcgagcgcc gccacccggc cctggcggtc ggcgcgctgc agcagctgct
      781 gcgagaccac tccgcgcagc gaggtggaca gcatggtgcg cacgtgcgac tgcttgtcgg
      841 aagggaacac attcaccatg cggtcgacgg tagcggccgc gccgttggtg tgcagcgtcc
      901 ccatcaccag aatgcccatc tccgcagccg tgacggcgat gctcatggtc tcgaggtcgc
      961 gcaactcgcc gaccagcacc acgtccggat cctcgcgcag ggccgagtgc accgccgcgg
     1021 cgaagctcgg actgtgcacg ccgatctcgc gttggctgat gaggcagttc tgtctctcgt
     1081 gcacgaactc gatcgggtcc tcgaccgtga ggatgtggcc gttcacgcgc cggttgatgt
     1141 catcgatcat ggcggccagt gtggtggact tgccggaccc ggtcttgccg gtcacgagga
     1201 tgagtccgct gttggcgcgg cacagcccgc gcaccgcctc cgggagcttg agctcatcga
     1261 gcgtcagcgc cttggaggga atcgcccgga acaccccgcc catgccgttc agctgccgca
     1321 tgacgttgac gcggaagcgc cccttgccct cgagcgtgta ggcgaagtcc gcgccgtcct
     1381 tcttctcgaa gcggtcgacc gcggtcttcg gcatgatctc gtagagcgcc gtcttgacgt
     1441 aatcggcgtt gagcttgtcc gggcgcagcg gcgtcaggtc gccgtacacg cggatgcgcg
     1501 gggagtcgcc ggcgatgaag tgcaggtccg agccgcgctt ctcgaggatc tcgtgcagaa
     1561 atggatcgat ctgcgacgcc acgtccggct cc
//
LOCUS       DATLKL010000566         1937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_142288, whole genome shotgun sequence.
ACCESSION   DATLKL010000566 DATLKL010000000
VERSION     DATLKL010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142288"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VM713_07605"
     CDS             complement(<1..494)
                     /locus_tag="VM713_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVH84163.1"
                     /translation="MSTEPYLLTPGPLTTSLRTRQSMLRDWGSWDVDFNSITGRVRER
                     LLQIVHGTATHECVPMQGSGTFSVEAAIGTLVPRAGHVLVPQNGAYCQRIARICRVLG
                     RKLTTVDYAENMQVDAADVERVLRTDASISHVALVHCETSTGILNPLQEVARVVARHG
                     RGLIV"
     gene            complement(507..1454)
                     /gene="phnX"
                     /locus_tag="VM713_07610"
     CDS             complement(507..1454)
                     /gene="phnX"
                     /locus_tag="VM713_07610"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016827 - hydrolase activity, acting on
                     acid carbon-phosphorus bonds [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0019635 - 2-aminoethylphosphonate catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HVH84164.1"
                     /translation="MARHLEIPSMSVISKLSSTGRVPDEIRQAAAQQQRFDQDNTENT
                     VGYVMLDCSGTTMDRYVDAPAIVFVEVFRKYGLEISMDEARKPMGLRKDLHIKAVTEI
                     PSVRERFRAKFGREPTQADVDKMFAVFVPTQIDLLKSGNYHELLPGTAEVVRKMQKDG
                     IRIGVTTGFTRGMLDILLAGGAQQGFNPDAACAGDEVEMPRPTAYMVIKNLERLGVLN
                     LKNAMRRTVKVDDTVSGAGEGAPLCWRVGVSKWSNYVVDSWAVARSLSPEQLAARERA
                     SKEKLITESGAHYVIDDLRDLPAVIAEINQRLARGESPD"
     gene            complement(1463..>1937)
                     /locus_tag="VM713_07615"
     CDS             complement(1463..>1937)
                     /locus_tag="VM713_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HVH84165.1"
                     /translation="GDLDPGAVLHVMERTGLSPPEMRAVLNGDSGLKGLAGGCADMQE
                     LLRRERQGDAGAGLAIEIFCRRARHYLGAFVCELGGVDVIVFGGGIGENSAEIRRRIV
                     HGLEWAGISIDDKANRAPDAGSIGAPDSGAAVEVVRVDEASVLADEAAALLERAA"
BASE COUNT          304 a          669 c          609 g          355 t
ORIGIN      
        1 acgatgaggc cccggccgtg gcgcgcgacg acccgggcga cctcctgcaa agggttcagg
       61 atcccggtgc tggtctcgca gtgcaccagc gccacgtgcg agatcgaggc gtcggtgcgc
      121 agcacgcgct cgacgtccgc ggcgtcaacc tgcatgttct cggcgtaatc gacggttgtc
      181 agcttgcgac cgagcacgcg gcatattcgg gcgatgcgct gacagtaagc cccgttttgc
      241 gggaccagga cgtgaccggc gcggggcacc agcgtgccga tggccgcctc gaccgagaac
      301 gtgccgctgc cctgcatcgg cacgcactcg tgcgtcgcgg tgccgtgaac gatctgcaac
      361 agccgctcgc ggacccggcc cgtgatgctg ttgaagtcga cgtcccagga tccccagtcg
      421 cgcagcatgc tctgccgggt gcgcaacgag gtcgtcagcg gtccgggagt cagtagatac
      481 ggttcggtgc tcatgaacgt gtcctatcag tccgggcttt ccccgcgggc cagccgttga
      541 ttgatctcgg cgatcaccgc cggcaggtcc cgcagatcgt cgatcacgta atgcgcgccg
      601 ctttcggtga tcagcttctc tttggaagct cgctcccgcg ccgccagctg ctccggactc
      661 agcgaccttg ctacggccca gctgtcgacg acgtagttgc tccacttcga tacgcccacc
      721 cgccagcaga gcggcgcgcc ctctccggcg ccggagacag tgtcgtccac cttgacggtg
      781 cggcgcatcg cgttcttgag gttcaacacg cccagacgct cgaggttctt gatgaccatg
      841 tacgcagttg ggcggggcat ttccacttcg tcgccggcgc aggcagcatc gggattgaat
      901 ccctgctggg cgccgccggc gaggaggata tccagcatgc cgcgggtgaa tccggtagtg
      961 acaccgatcc ttattccgtc cttctgcatc ttgcggacga cctccgccgt gcccggcaag
     1021 agctcgtggt agttgccgct cttcaggagg tcgatctgag tcggaacgaa taccgcgaac
     1081 atcttgtcca cgtctgcctg cgtcggctcc cggccgaact tcgccctgaa gcgctcgcgc
     1141 actgacggaa tttcagtgac cgccttgatg tgcaggtcct tgcgcagacc catgggcttg
     1201 cgcgcctcgt ccatgctgat ctccaggccg tatttcctga acacctcgac gaatacgatg
     1261 gccggcgcat cgacgtagcg gtccatcgta gtgccagagc agtcgagcat cacgtagccg
     1321 acggtgttct cggtattgtc ctgatcgaac cgctgctgct gcgccgcggc ctggcggatt
     1381 tcgtcaggca cgcgccccgt tgaagaaagc ttactgatca cgctcatcga aggaatctcc
     1441 agatgccggg ccaattagat cttcaggcgg cgcgttcgag cagcgccgcc gcttcgtccg
     1501 ccagtacgct cgcctcgtcg acgcggacca cctcgacggc ggccccgctg tccggcgcgc
     1561 caatgctgcc cgcgtcgggc gcgcgattgg ctttgtcatc gatgcttatg ccggcccact
     1621 cgaggccgtg gacgatgcgg cggcggatct cggcgctgtt ctcgccgatg ccgccgccga
     1681 agacgatgac atcgactccg ccgagctcgc acacgaaggc ccccaggtag tgccgcgccc
     1741 gccgacagaa tatctcgatc gcgagcccgg cacccgcatc gccctggcgc tcgcggcgca
     1801 gcagctcctg catgtcggcg cagccacccg cgaggccctt caggccgctg tcgccattga
     1861 gcaccgcacg catctcgggg ggtgagaggc ccgtgcgctc catgacgtgc agcacggcac
     1921 cgggatccag gtctccc
//
LOCUS       DATLKL010000567         1791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_164662, whole genome shotgun sequence.
ACCESSION   DATLKL010000567 DATLKL010000000
VERSION     DATLKL010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164662"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1791)
                     /locus_tag="VM713_07620"
     CDS             complement(<1..>1791)
                     /locus_tag="VM713_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84166.1"
                     /translation="CRLDARGGQLKHIVYIQFDNVHLRRDNPNVPSDLEQIPSLRHFL
                     EDGGTLLTNHHTPLISHTSVDIVTSLTGVYGDKFGVPIGNSIGLFNAATGVASFPSSF
                     TYWTDLLADGTPEMLAQTGKNAPAPWVPFTRAGCDVGAFSIANIEFENIGSDINNVFG
                     PNSPQAQEAASNFRKAIADFEGIIVHCAQGSALCAQHSAPDILNDEPGGYLGYTALYG
                     NANVQPQISPSGPVLDLDRNPITDGHGNNGFPGFDPSPSQTLGYLATMLEAGVPVVYG
                     YIEDAHDNHGAQISGVSPSVEETFGPGEAGYVTQLKAFDRAFGQFFARLAADGITRDN
                     TLFIVTADENDHFAGSGPSPVDCDGVTTPCSYAHKGEIDADLDAVLFTEFGDATPFRI
                     HFDDAATFYINGNPAQTDPKTRQLERESSVALGFDVIQGGDNLLAQALADQAEQALLH
                     MVSADPNRTPNFILFGNPDYFFLTSGKSPPPACTPANDAASCFAEGAGFAWNHGDFQK
                     EITRTWLGMAGPGVAQLGRFGQIFTDHTDIRPTLIYLAGLSDDYGHDGRVLFETLTPA
                     ATDAALQAHRDTLSALAAAYKEINAPLGKLG"
BASE COUNT          283 a          531 c          659 g          318 t
ORIGIN      
        1 ccgagcttgc cgagcggcgc gttgatttcc ttgtaggcgg cagccagcgc cgacagcgta
       61 tcccgatgtg cctgcagggc ggcgtccgtc gccgcgggcg tcagcgtctc gaacagtacg
      121 cggccgtcgt gcccgtagtc gtcgctcagg ccggcgaggt agatgagcgt cgggcggatg
      181 tccgtgtggt cggtgaagat ctgcccgaac ctgccgagct gcgccacgcc ggggcctgcc
      241 ataccgagcc acgtccgggt gatctctttc tgaaaatcgc cgtggttcca ggcgaaaccc
      301 gcgccctcgg cgaagcagct ggccgcatcg ttcgccggag tgcacgccgg cgggggcgac
      361 ttcccggagg taaggaagaa ataatccggg ttgccgaaga ggatgaagtt cggcgtgcgg
      421 ttcggatcgg cactcaccat gtgcagcagc gcctgctccg cctggtcggc gagcgcctgc
      481 gcgagcaggt tatcgccccc ctggatcacg tcgaagccga gcgcaacgct cgactcgcgc
      541 tcgagctggc gggtcttcgg gtcggtctgc gcggggttgc cgttgatgta gaaggtcgca
      601 gcgtcgtcga agtggatgcg gaagggcgtg gcatcgccga actccgtgaa cagcaccgca
      661 tcgaggtcgg catcgatctc gcccttgtgc gcgtagctgc acggagtcgt gacgccgtcg
      721 cagtcgaccg gggaggggcc cgagccggcg aagtggtcgt tctcgtcggc tgtgacgatg
      781 aagagcgtat tgtccctggt gatgccgtca gcggcgagac gtgcgaagaa ctggccgaac
      841 gcccggtcga aggccttgag ctgcgtgacg tagccggcct cgcccggccc gaaggtctcc
      901 tcgaccgagg ggctgacgcc cgagatctgc gcgccgtgat tgtcatgggc gtcctcgatg
      961 tagccgtaca cgaccggcac gccggcctcg agcatggtcg cgaggtagcc gagcgtctgt
     1021 gagggcgagg ggtcgaaccc ggggaagccg ttgttgccgt ggccgtcggt gatcggattc
     1081 cgatcgagat cgaggaccgg gccgctcggc gagatctgcg gctgcacatt ggcgttgccg
     1141 tagagcgccg tatagccgag gtagccgccc ggctcgtcat tgaggatatc gggggcgctg
     1201 tgctgcgcgc acaaggcgct cccttgcgcg cagtgcacga tgatgccttc gaaatcggcg
     1261 atcgccttgc ggaagttgct ggcggcctcc tgcgcctggg gcgagttggg gccgaagacg
     1321 ttgttgatgt cgctgccgat gttctcgaac tcgatgttgg cgatcgagaa ggcgcccacg
     1381 tcgcagccgg cgcgggtgaa cggcacccag ggcgccgggg cgttcttgcc ggtctgagcc
     1441 agcatctcgg gcgtgccgtc ggcgagcagg tcggtccagt aagtaaagga gctcgggaag
     1501 ctcgcgaccc cggtcgccgc gttgaagagc ccgatgctgt taccgatggg cacgccgaac
     1561 ttatcgccat acacgccggt cagcgaggtc acgatgtcga ccgaagtgtg cgagatgagc
     1621 ggcgtgtgat gattggtgag cagggtgccg ccgtcctcga gaaagtgccg cagactcgga
     1681 atctgctcga ggtcggatgg cacgttgggg ttgtcgcgcc gcaagtgcac gttgtcgaac
     1741 tggatataga cgatgtgctt gagctggccg ccgcgggcat cgaggcggca t
//
LOCUS       DATLKL010000568         1652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_108284, whole genome shotgun sequence.
ACCESSION   DATLKL010000568 DATLKL010000000
VERSION     DATLKL010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108284"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VM713_07625"
     CDS             <1..899
                     /locus_tag="VM713_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HVH84167.1"
                     /translation="VDFYHRRFSLLVCTTIIESGIDVPSANTIMINRADHMGLAQLHQ
                     LRGRVGRSHHRAYAYLIAPPRQALPRDAAKRLEAIESMEELGAGFVLATHDLEIRGAG
                     ELLGEQQSGNMTEIGLSLYLDMLDHAVRALKSGRAPALDQPLAAATEVELRLPAFLPE
                     AYVADVHVRLSLYKRIAAAESAAALDELTAELHDRFGPLPAAAVSLIRVAKLKLAART
                     LGVRRLDLGPQGGSVLFEERTSVDPASLVRLIQRSSREYRLDGPRRLRISRQMPTDQA
                     RFDYAAELLGRLGAAAHPPPPG"
     gene            910..1542
                     /locus_tag="VM713_07630"
     CDS             910..1542
                     /locus_tag="VM713_07630"
                     /inference="COORDINATES: protein motif:HMM:NF022425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HVH84168.1"
                     /translation="MDRPTAPLRTLALALAAALGAALPAASQESAGPVYNVEIIVFRT
                     ATADGVPENWSEEAGARSIAGDESASGSVQVGRFAGLLPPAAGQLNELESRLRASGTY
                     LPVAHAAWSQTASSWGTRAGFAVGRLGIDVPGLSGNVYLEHGQFLHLGLSLTWAMQNP
                     PDGLGAASGTAFTLNETRRVRFYERNYYDHPAFGVIALVTPAQGPRAPGR"
BASE COUNT          231 a          606 c          562 g          253 t
ORIGIN      
        1 tggtggattt ctaccatcgg cgcttcagcc tgctggtgtg caccaccatc atcgagagcg
       61 gcatcgacgt gccgagcgcc aacaccatca tgatcaaccg cgccgatcac atgggactcg
      121 cgcagctgca ccagctgcgc gggcgcgtcg gccgctccca ccaccgcgcc tatgcctatc
      181 tcatcgcgcc gccccgtcag gcgctgccgc gcgatgccgc caagcggctc gaggccatcg
      241 agtcgatgga ggagctcggc gcgggcttcg tgctcgcgac ccacgacctc gagattcgcg
      301 gcgcgggcga gctgcttggc gagcagcaga gcggcaacat gaccgagatc ggcctgtcgc
      361 tctatctcga tatgctggac cacgcagtgc gcgcgctcaa gtccgggcgc gcgcccgcac
      421 tcgatcagcc gctcgcggcg gcgaccgagg tggagctgcg cctgccggcg ttcctgcccg
      481 aggcctacgt ggccgacgtg catgtgcggc tttccctcta taagcgcatc gccgcggccg
      541 agagcgccgc ggcgctcgat gagctgaccg ccgagctcca cgatcgcttc ggcccgttgc
      601 cggcggcggc ggtgagcctg atccgcgtcg cgaagctgaa gctcgccgcg cgcacactgg
      661 gcgtgcgccg tctcgacctg ggaccccagg gaggctcggt gctgttcgag gagcgcacca
      721 gcgtcgatcc ggccagcctg gtacgcctga ttcagcggag ctcgcgcgaa taccgtctgg
      781 atggcccccg caggttgcgc atatcgcgcc agatgcccac cgatcaggcg cgcttcgact
      841 acgcggccga gctgctcggc cggctcggcg cggcggcaca cccgccgccg cccggttaga
      901 atgccgcgca tggatcgccc caccgcaccg ctgcgcacgc tcgcgctcgc gctcgccgcc
      961 gcgctcggcg ccgctctccc cgcggcctcg caggagagcg ccggccccgt ctacaacgtc
     1021 gagatcatcg tgttccgcac cgctaccgcc gacggtgtcc ccgagaactg gagcgaggaa
     1081 gccggcgcgc gcagcatcgc cggcgacgaa tcagcgagtg gctcggtgca ggtgggccga
     1141 ttcgcaggcc tgctgccgcc cgccgccggg cagctcaacg agctcgagag ccggctgcga
     1201 gcgagcggca cctacctgcc ggtggcgcac gccgcctggt cgcagaccgc gagctcctgg
     1261 ggcacgcgcg ccggctttgc ggtcggacgc ctcgggatcg acgtacccgg gctcagcggc
     1321 aacgtgtacc tcgagcacgg gcagttcctg cacctcgggc tgtcgctgac gtgggcgatg
     1381 cagaatccgc cggatggact cggcgccgcc tccggcacgg cgttcacgtt gaatgagacg
     1441 cgccgcgtgc gcttctacga gcgcaactac tacgatcatc cggccttcgg tgtgatcgcc
     1501 ctcgtgacgc ccgcacaggg cccgcgcgct ccgggtcgat aggccaaaat cgttggcgca
     1561 cgggtttttg ccggctgcgc agtgccggtg ccagcgttac tggaagtcgg cggcctcggc
     1621 cgtggcaatg ccgtccttgc cgttggtctt gg
//
LOCUS       DATLKL010000569         1746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_153717, whole genome shotgun sequence.
ACCESSION   DATLKL010000569 DATLKL010000000
VERSION     DATLKL010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153717"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VM713_07635"
     CDS             <1..885
                     /locus_tag="VM713_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84169.1"
                     /translation="PAGRLTAEQKLRKECGFSQILVDDLKAHRLQITRAYLAGNFEAA
                     FDLALYAVCTDLFRHVGYHTNPLDLRATECAPRSSLNDLSGTPADRLLEAQRSALDLD
                     WLQLPPSKAFGALAALPMDAKQRLFAWCVASCLKPQLAIEDRADPAIECAGWRLAIPF
                     ADCWRPTAANYWSRVRKAHGLAIGAELLGERWARDHAGDKKPVLAAALQNAFDPALSD
                     SCIGLAQSARDSAAAWLPPGMAYDEKATASHSAEPDPGLDEANLADGGAEDGSADLPA
                     FLTEDEPAITALNGAAAH"
     gene            1095..1331
                     /locus_tag="VM713_07640"
     CDS             1095..1331
                     /locus_tag="VM713_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84170.1"
                     /translation="MLETIFGPTISLSTGRVIPTRRVGEHHFQQHLGFIPSFADWMRA
                     SGLCPRWAVPARLEDRPHRAATLPADSGERRDQS"
     gene            1467..>1746
                     /locus_tag="VM713_07645"
     CDS             1467..>1746
                     /locus_tag="VM713_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3846 domain-containing protein"
                     /protein_id="HVH84171.1"
                     /translation="MRAIIIDAKHRTITVTDIDGSLKSLQQIVGGLIEPVTQGLDEFH
                     HCYVNEEGLHDQPQHFFMFNGGHQPLAGNGVILSSTDDGDEAPCTLLLD"
BASE COUNT          305 a          635 c          532 g          274 t
ORIGIN      
        1 ccggcgggcc gcctcaccgc cgagcagaag ctgcgcaagg aatgcggctt cagccagatc
       61 ctggtcgacg atctcaaggc tcatcgcctg cagatcaccc gggcatatct ggcggggaat
      121 ttcgaggccg cattcgatct cgccctctat gctgtgtgca ctgatctctt caggcacgtc
      181 ggctaccaca ccaacccgct cgatttgcgg gcaacggagt gcgcgccgcg cagctcgctc
      241 aacgatctct ccggcacgcc ggccgaccgc ctcctcgagg cgcagcggag cgctctcgat
      301 ctcgactggc tgcaattgcc gccgtccaaa gccttcgggg cgctcgccgc gctgcccatg
      361 gacgccaagc agcgcctgtt cgcctggtgc gtcgccagct gtctcaagcc gcagctcgcg
      421 atcgaggacc gcgcagatcc ggccatcgaa tgcgccgggt ggcgattggc gatcccgttc
      481 gccgactgct ggcggccgac ggccgctaat tactggagcc gcgtgaggaa agcgcacggt
      541 ctcgccatcg gcgccgaact cctcggcgaa cgctgggcgc gcgaccacgc cggcgacaaa
      601 aagccggtgc tcgccgcggc gctgcaaaac gcctttgatc cggcgctcag cgacagctgc
      661 atcggcctcg cgcagtccgc gcgcgatagc gccgcagcgt ggctgccgcc cggcatggcc
      721 tatgacgaga aggccacggc cagtcattca gcggagccgg atcccgggct cgatgaagcg
      781 aacctcgcgg acggcggcgc cgaggacggg tcagcggatt tgcccgcctt cctgaccgag
      841 gacgaacctg cgatcaccgc gctcaacggc gcggccgccc actgaggtgc actgaaaccg
      901 gagaccgcgc accggcgggc gcgcggcctt cgccattcgt aacgcccgcc aaggcccggg
      961 ctcctcttca aacggtgtgg gctgaaccgc acacgccgga acctgaacgt cggtcatgcc
     1021 ggccccacca accaagctga gtgccccgcg gacactatgc ctggccccgt ttgatggcag
     1081 aggctgtcca tataatgctc gagacgatct tcggcccgac gatcagcttg tcgaccggcc
     1141 gcgtgatccc gacccgccgg gttggcgaac accatttcca gcagcatctc ggcttcatcc
     1201 cgtccttcgc cgactggatg cgcgcatccg gcctctgccc tcgatgggcc gtgccggccc
     1261 gcctcgagga ccggccccac cgcgcagcaa cgctacccgc cgacagcggc gagcggcgcg
     1321 atcagtcgtg atcgggccgc agctccctat ccggccgcga gagtgagagg cacgccggga
     1381 agggatgaac cgtgcgcgca aagagagcgc ctcggttcct ccccggatca ggtccggggc
     1441 aggctcaacc tcccggaggc ctcgccatgc gcgcgatcat catcgacgcc aaacaccgca
     1501 cgatcaccgt cactgacatc gacggctcgc ttaaatccct tcagcagatt gtcggcggat
     1561 tgatcgagcc cgtcacccag ggtctcgacg aattccacca ttgctatgtc aatgaagaag
     1621 ggctgcacga tcagccgcag catttcttca tgttcaacgg cggccaccaa ccgctcgccg
     1681 gcaatggcgt catcctttca tccaccgatg acggcgatga agcgccctgc acccttctgc
     1741 tcgatt
//
LOCUS       DATLKL010000570         2699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_198658, whole genome shotgun sequence.
ACCESSION   DATLKL010000570 DATLKL010000000
VERSION     DATLKL010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198658"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1371
                     /locus_tag="VM713_07650"
     CDS             196..1371
                     /locus_tag="VM713_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HVH84172.1"
                     /translation="MPRPRFIDPFQPAEVRRLVREFGSPLLILDCQRVRVQFRKLRKA
                     LPHVDLHYALKPLPHPAVVRTVIEEGGYLDLATSGEVQLVEQLGISPERCIHTHPIKR
                     PADIANALEYGVRTFVADNPDEVRKFARHRDAAQLLLRVSFRSPGAVCDLSRKFGCDP
                     EGVLELARIAAGLGVGVRGLSFHAGSQTPDATKHVEALEACAALMAAARRDKLGVFDT
                     LDIGGGFPIDYTQPVQDIGRFCAPLRAALARLPKRVRVIGEPGRYIVGPAAIGVASVM
                     GRAQREGHWWYYLDDGLYGSYSGQLYDHARYPVETLRDGAERLPSVLAGPTCDSIDVI
                     AENLMLPPLEAGDLIIGRAMGAYTRASATEFNFFPRATVVAVNTRPGDGGGVDPLAL"
     gene            1442..2050
                     /locus_tag="VM713_07655"
     CDS             1442..2050
                     /locus_tag="VM713_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HVH84173.1"
                     /translation="MKSPAIRNVVFDIGWVLMRLKYQPLLELLRSRGVEGDDLHAVLA
                     RAALEEHESGRLHGLGLLERLAALTRDPVSIEELYAYWIDMFELEPQMVELAHRLSEA
                     HRVYLLSNIGDLHWAYLSREHRFHRIGHGALLSFLAGVMKPQAGIYAEAERRFALEPA
                     ATVFIDDRADNIATARRRGWHGIVHGGYQSTLRELRGLDVAC"
     gene            2061..2573
                     /locus_tag="VM713_07660"
     CDS             2061..2573
                     /locus_tag="VM713_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HVH84174.1"
                     /translation="MKHDTPAQPLTRIHQQVNEARAGRNPRVIARLYSGWAVFGERQF
                     VRGYCLLLPDPVVANLNALDAQERTTFLTDMARLGDALLKVTAALRINYAMFGNEEPA
                     LHAHVIPRYLDEPEALRAAHPWAYDWKAAPMFERTACAELAESLLHELQRLGVTKPMR
                     FAPGTDSAKS"
BASE COUNT          396 a          967 c          927 g          409 t
ORIGIN      
        1 cgggcacgac ctttacaaca atctgcgcac cctcgaccgc gccggctgcc agcgcatcct
       61 ggtacaggag gtgcccgagg gtgagcgctg ggacgccatc cgtgaccgcc tgctgcgcgc
      121 cgccacgagc ttcgcgcaca gcgatgaagg cagcggcacg atggcggtgc tgccgtaagt
      181 ccgattgctg ccccggtgcc gcgcccgcga ttcatcgacc cgttccagcc cgcagaagtg
      241 cgccgactcg tgcgcgagtt cggctcaccg ctgctgatcc tcgactgcca gcgcgtgcgg
      301 gtgcagttca ggaagctgcg caaggcgctg ccgcacgtcg acctgcacta cgcgctcaag
      361 cccctgccgc acccggcggt ggtgcgcacg gtcatcgaag agggcggcta cctcgacctc
      421 gcgacgagcg gcgaggtgca gctggtcgag cagctcggca tatcccccga gcgttgcatc
      481 cacactcatc ccatcaagcg ccccgcggac atcgccaatg cgctcgagta tggcgtgcgg
      541 accttcgtcg ccgacaaccc ggatgaggta cgcaagttcg cccgccaccg cgacgcggcg
      601 cagctgctgc tgcgcgtctc gttccgctca cccggcgcgg tgtgcgacct gtcgcgcaag
      661 ttcggctgcg acccggaagg cgtgctcgag ctggcgcgga tcgcggccgg tctcggggtg
      721 ggcgtgcggg gactctcgtt ccacgccggc tcgcagactc cggacgccac caagcacgtc
      781 gaggcgctcg aggcgtgcgc cgctctcatg gcggcggcgc gccgcgacaa gctcggcgtg
      841 ttcgacacgc tcgacatcgg cggcggcttt ccgatcgact acacacagcc agtgcaggac
      901 atcgggcgct tctgcgcgcc gctgcgggca gcgctcgcgc ggctgccgaa acgggtgcgc
      961 gtgatcggcg agcccgggcg ctacatcgtc gggcccgccg cgatcggcgt cgcctcggtg
     1021 atgggacgcg cgcagcgcga gggccactgg tggtactacc tcgacgacgg gctgtacggc
     1081 tcctacagcg gccagctgta cgatcacgcg cgctaccccg tggagacgct gcgcgatggc
     1141 gccgagcgac tgccctccgt gctcgccggc cccacctgcg acagcatcga cgtgatcgcc
     1201 gagaacctga tgctcccgcc gctcgaggcg ggcgacctga tcatcgggcg cgcgatgggc
     1261 gcctacacgc gcgcgagcgc gacggaattc aacttctttc cgcgcgccac cgtggtggcg
     1321 gtcaacaccc ggccgggcga cggcggcggg gtcgacccgc tggcactctg agcgggactt
     1381 tcatgaacga ctcgaccccg ctgcggcagc cacgattgaa ttcgtctttc ccggggcgtt
     1441 catgaagagt cccgcgatcc gtaatgtggt gttcgacatc ggctgggtgc tgatgcggct
     1501 caagtaccag ccgctgctcg agctgctgcg ctcgcgcggg gtcgagggcg acgacctgca
     1561 cgcggtgctc gcgcgcgccg cgctcgagga gcacgagtcg ggccggctgc acggcctggg
     1621 cctgctcgag cggctggcgg cgctgacgcg cgatccggtg tcgatcgagg agctgtatgc
     1681 ctactggatc gacatgttcg agctcgagcc gcagatggtg gagctggccc accgcctgag
     1741 cgaggcgcat cgcgtctacc tgctgtcgaa catcggtgac ctgcactggg cgtatctgtc
     1801 gcgcgagcac cgcttccacc gcatcgggca cggtgcgctg ctctcatttc tcgccggtgt
     1861 catgaagcct caggcgggca tctacgccga ggcggagcgg cgcttcgccc tcgagccggc
     1921 ggcgaccgtg ttcatcgacg accgcgccga caacatcgcc accgcgcgcc gccgcggctg
     1981 gcatggcatc gtccacggcg gctaccagag caccctgcgc gagctgcgcg ggctcgacgt
     2041 ggcctgctga agcccgcctc atgaagcacg acaccccagc gcagccgctc acccggatcc
     2101 accagcaggt gaacgaggcg cgtgccgggc gcaatccgcg cgttatcgcg cgcctctact
     2161 cgggctgggc ggtgttcggc gagcggcagt tcgtgcgcgg ctactgtctg ctgctgcccg
     2221 atccggtggt tgccaatctc aacgcgctcg atgcccagga gcgcaccaca ttcctcaccg
     2281 acatggcgcg tctcggcgat gcgctgctca aggtgaccgc tgcgctgcgc atcaactacg
     2341 cgatgttcgg caacgaggaa ccggcgctgc acgcgcacgt catcccgcgc tatctcgacg
     2401 agcccgaggc gctgcgcgcg gcgcatccct gggcgtacga ctggaaagcg gcgcccatgt
     2461 tcgagcgcac ggcctgcgca gagctcgccg agagcctgct gcacgagctg cagcgcctcg
     2521 gggtgacgaa gccgatgcgt ttcgcgcccg gcaccgacag cgcgaagtcc tgaaggcgcc
     2581 ggcggcgccc cggacggccg tcgcagccgc ggctgctact tcggcggcgg ctgagccggc
     2641 gtgggcggtg ccgcgggctt gaagctcacc agctccacct cgaacacgag catcgagcc
//
LOCUS       DATLKL010000571         2682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_175881, whole genome shotgun sequence.
ACCESSION   DATLKL010000571 DATLKL010000000
VERSION     DATLKL010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175881"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..461
                     /locus_tag="VM713_07665"
     CDS             177..461
                     /locus_tag="VM713_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVH84175.1"
                     /translation="MRRGQLTLDAERMQVEWDGKVVVLSLTEFWIVHALARHPGHVKN
                     RQQLMDAANVVLDDNTITSHVRRIRRKFQGVDPQFDAVQTVYGMGYRWVE"
     gene            472..2457
                     /locus_tag="VM713_07670"
     CDS             472..2457
                     /locus_tag="VM713_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH84176.1"
                     /translation="MSLRLKLLLLGLLTLVLPWAGCRYAREMETALREGEQTSLESVA
                     ETLATSLEGRVDLLYRAPPGFDATDTAALLVAGPYDLKPLALTAAPLVDGYSEDWPHD
                     PAAWAWYAKDAGHRFGVLSGVFERVLYLLLDVRDAHPVFDAPGASPLDAHATGDRVWL
                     GFQDPEGTEREVFIAATGPGAVTARYIETGEYGQQLAQLEPRIRGAWQLSPGGYRLAL
                     RVPLSMLGTGFGVLVDDRDARGAAPVSYGSLHPDDLRTIGHLIVASPDLTTYLAQFSQ
                     PGLKLAVSTPAGRVLARVDELRRVAELGPAPPLIARLYRRFVDQPGTAPPIEARVPIY
                     DRGHALAIGALEVTQTPDRWLGLRDRALTHMLNFTLATSLVVVIAMFAFAAWLALRLS
                     RLRQASESALTRTGLVATFPDTGARDELGDVARSFSSLLGRLNEYTGYLRTLAGKLAH
                     EIRTPLTIVRSSLENLEAEQVPASARAYLERARHGSERLNAILLAMGAATRVEEAIGA
                     AERSHFDLLPPLESAVAAYRGAFPERRFAAELPPGPVMMDGAPDLIVQMLDKLVENAV
                     DFSAPGATITVRLLLTPQAALLEVDNPGPPLPSEAPGRLFESLWQSRSEGDNRLHFGL
                     GLYIVRLIAEFHGGSASAGNLPGGRGARFSVRLPLPI"
BASE COUNT          388 a          958 c          898 g          438 t
ORIGIN      
        1 tccgccgcaa gtttcaggcg ctcgatccca agtttgatgc ggtacagacc gtctacggaa
       61 tgggctatcg gtgggtcgag tgagccggct cgggcgcgcg ctttgagcgc gcatgaaccg
      121 cgctgcctca gtgccgcata gaggccctgc gccgaccgct cgacgacggc caggtcgtgc
      181 gccgcggcca gctgacgctc gacgccgagc gcatgcaggt cgagtgggac ggcaaggtcg
      241 tggtgttgtc gctcaccgag ttctggatcg tgcacgccct cgcgcgacac cctggccacg
      301 tgaagaatcg ccagcaactc atggacgccg ccaacgttgt gttggatgac aacacgatca
      361 cctcgcacgt gaggcgcatc cggcgcaagt tccagggcgt cgatccgcag ttcgacgccg
      421 tacagaccgt ctacggcatg ggctaccgct gggtcgagta gccccctgac gatgtcgctg
      481 cgcctgaagc tgctgctgtt gggcctgctc acgctggtgc tcccgtgggc gggctgccgt
      541 tacgcgcgcg agatggaaac ggcgctgcgc gagggggagc agacgtccct cgagtcggtc
      601 gccgagacgc tggcgacctc gctcgagggt cgggtggacc tcctctaccg cgcgccgccg
      661 gggttcgacg ccacggacac cgccgcgctc ctcgtggcgg ggccctacga cctgaagccc
      721 ctggcgctta ccgcggcgcc gctcgttgac ggctattccg aggactggcc gcacgatccg
      781 gcggcctggg cctggtatgc gaaggatgcc ggccaccgct tcggcgtgtt gagcggcgtt
      841 ttcgagcgcg tgctctacct gctgctcgac gtgcgcgacg cgcacccggt cttcgacgcc
      901 ccgggcgcga gcccactcga tgcgcacgcc accggcgacc gcgtctggct cggcttccag
      961 gacccggaag gcaccgagcg ggaggtgttc atcgccgcga ccggcccggg tgcggtcacg
     1021 gcccgctaca tcgagaccgg cgagtacggc cagcagctcg cgcagctcga gccgcgcatc
     1081 cgcggcgcct ggcagctgag ccccggcggc tatcgtctcg cgctacgcgt gccgctctcg
     1141 atgctcggca cgggcttcgg cgtgctcgtc gacgaccgcg acgcgcgcgg cgccgcgccg
     1201 gtgagctacg gcagcctcca tcccgacgat ctgcgcacca tcggccacct gatcgtggcc
     1261 tcgcccgatc tcacgaccta cctcgcgcag ttctcgcagc cgggactgaa gctcgctgtg
     1321 tcgacccccg ccgggcgtgt gctcgcgcgc gtcgacgagc tcaggcgcgt cgcggagctc
     1381 ggacccgcgc cgcccctgat cgcgcggctc taccggcgct tcgtcgatca gcctgggacg
     1441 gcaccgccga tcgaggcgcg ggtgccgatc tacgaccgcg gccacgccct ggcgatcggc
     1501 gcgctcgaag tgacgcagac gcccgaccgc tggctgggct tacgcgatcg cgccctcaca
     1561 cacatgctca acttcacgct cgcgacctcg ctcgtggtcg tcatcgccat gttcgcgttc
     1621 gctgcctggc tggcgctgcg gctgtcgcgt ttgcgccagg cgagcgagtc ggcgctgacc
     1681 cgcactggtc tcgtcgcgac cttccccgac accggggcgc gcgatgagct cggcgacgtg
     1741 gctcgcagct tctcaagtct cctcggccgg ctgaacgagt acacgggcta cctgcgcacg
     1801 ctcgccggga agctcgccca tgagatccgc acgccgctca ccatcgtgcg ctcctcgctc
     1861 gagaacctgg aggccgagca ggtgccggcc tccgcccgcg cctatctcga gcgcgcccgc
     1921 cacgggagcg agcggttgaa cgcgatcctg ctagccatgg gcgccgcaac ccgcgtcgag
     1981 gaggcgatcg gggccgcaga gcgcagccac ttcgatctcc tcccgccgct cgagtcggcc
     2041 gtggcagcct accgcggcgc cttccccgag cggcgcttcg ccgccgagct tcctcccggg
     2101 cccgtgatga tggacggtgc cccggatctg atcgtgcaga tgctcgacaa gctcgtcgag
     2161 aacgccgtcg atttcagcgc accgggggcc accatcacgg tgcggctcct gcttacaccc
     2221 caggcggccc tgctggaggt cgacaacccg ggaccccctc tgccttccga ggccccgggg
     2281 aggctttttg agtcgctgtg gcagtcccgc agcgagggcg acaaccgcct gcattttggc
     2341 ctcgggcttt acattgtgcg gctgatcgcc gagttccacg gaggatccgc atccgccggc
     2401 aacctccccg gggggagggg ggcgcgcttc tccgtgcgac tgcccctgcc tatttgaccc
     2461 catgccgggt cggcaaaaga ccccccgggt gtcgcctttg tgctacatgg ttttctgcct
     2521 tggaatcagt ataatttata gacggaaggc accgtccgtt aagggtttta gactccccac
     2581 ggggagcgag cacagcggcg gtgatgcgta caagcaaaca gctgaaagga ctaagaacag
     2641 agaaatctcg gggatcgacg cgggcacggg tgagacagcg gc
//
LOCUS       DATLKL010000572         1627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_220981, whole genome shotgun sequence.
ACCESSION   DATLKL010000572 DATLKL010000000
VERSION     DATLKL010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220981"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VM713_07675"
     CDS             complement(<1..584)
                     /locus_tag="VM713_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVH84177.1"
                     /translation="MTFIAKPKVAHPSLPRNALGFTRRDYEGALSTLCAGCGHDSITA
                     AIVEASWALSLEPQNVVKLSGIGCSSKTTAYFVSGGHGFNSVHGRMPSIAMGANAANR
                     DLTYIGVSGDGDTLSIGLGQFCHAIRRNVDMLYIIENNGVYGLTKGQFSASADIGTKA
                     KKGETNLQPPIDPVLLAMTLGASFVARSFSGDKQ"
     gene            complement(621..>1627)
                     /locus_tag="VM713_07680"
     CDS             complement(621..>1627)
                     /locus_tag="VM713_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84178.1"
                     /translation="IGAMWNGARAFTATSGPGISLMNEFLGLAYYAEVPVVIFDIQRV
                     GPSTGMPTRTQQGDLLLAAYASHGDTRHVCLYPANPEECFYMAAQAFDLAERLQTPVL
                     VLSDLDIGMNDWMCRDLKWDDNYRPDRGKVLGKEEVLKLKKFYRFLDVDDDGIGYRTL
                     PGVDPKAAYFTRGSGHNQYGAYAEDLAEYQIVLDRLMRKWATAKRLVPRALIDATADS
                     KIGLVSLGSCDGAIREARDLLSQRGVVVDYMRVRSFPFSEDVEKFLAAHELLFVVEQN
                     RDAQLRSLLTLETAVEKSKLRSLLHYSGLPISSAFIVDGVLAELQTERRVQPRESAHG
                     "
BASE COUNT          273 a          507 c          576 g          271 t
ORIGIN      
        1 tgctgcttgt cgccggagaa gctgcgcgcc acgaaggacg ccccgagcgt catggcgagc
       61 agcaccggat cgatcggcgg ctggagattg gtctctccct tcttcgcctt ggtgccgatg
      121 tcggcggagg ccgagaactg ccccttggtg aggccgtaca cgccgttgtt ctcgatgatg
      181 tagagcatgt cgacattgcg gcggatggcg tggcagaact ggccgagccc gatcgagagc
      241 gtgtcgccat cgccggagac gccgatgtag gtgaggtcgc ggttcgcagc gttcgccccc
      301 atggcgatcg acggcatgcg cccgtgcacg gagttgaagc cgtgcccgcc gctgacgaag
      361 taggcggtgg tcttcgagga acaaccgatc cccgagagct tcaccacgtt ctgcggctcg
      421 agcgacagag cccagctcgc ctcgacgatc gccgcggtga tcgagtcgtg gccgcagccg
      481 gcgcacagtg tcgagagcgc cccctcgtag tcgcgccgcg tgaacccgag cgcgttgcgg
      541 gggagcgagg ggtgggcgac cttgggtttg gcgataaaag tcatgcagtg gctccggacg
      601 acgggaatcg ctgcggctgc tcagccatgg gccgactctc gcggctgcac gcgccgctcg
      661 gtctgcagct ccgcgagcac cccatccacg atgaacgcgg acgagatggg cagccccgag
      721 tagtgcagca gcgagcgcag cttcgacttc tcgacggcgg tctcgagtgt gagcagcgag
      781 cgcagctgcg cgtcgcggtt ctgttcgacg acgaacagca actcgtgcgc ggcgaggaac
      841 ttctcgacgt cctcgctgaa cgggaaggag cgcacgcgca tgtagtccac gacgacgccg
      901 cgctgcgaaa gcaggtcgcg cgcctcgcgg atcgcgccgt cgcagctgcc gagggacacg
      961 aggccgatct tcgagtccgc ggtcgcatcg atgagcgcgc gcggcaccag gcgtttcgcc
     1021 gtcgcccact tgcgcatcag ccgatcgagc acgatctggt actcggccaa gtcctcggcg
     1081 tacgcgccgt actggttgtg gcctgagccg cgggtgaagt aggcggcctt gggatccacg
     1141 cccgggaggg tccggtagcc gatgccgtca tcgtccacgt cgaggaagcg gtagaacttc
     1201 ttgagcttga ggacctcttc cttgccgaga accttgccgc ggtcggggcg gtagttgtcg
     1261 tcccacttga ggtcgcggca catccagtcg ttcatgccga tatcgagatc ggagagcacc
     1321 agcaccggag tctgcagccg ctcggcgagg tcgaaggcct gcgccgccat gtagaagcat
     1381 tcctcggggt tggccgggta gaggcacacg tggcgggtgt ccccgtggga agcataggcg
     1441 gcgagcagca gatcaccctg ctgggtgcgg gtcggcatgc cggtcgatgg accgacgcgc
     1501 tggatgtcga agatcactac cggaacttcc gcgtaatagg cgaggccgag gaactcgttc
     1561 atgagcgaga tgccgggtcc cgaagtcgcg gtgaaggcgc gcgcaccgtt ccacatcgcg
     1621 ccgatcg
//
LOCUS       DATLKL010000573         2118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_17993, whole genome shotgun sequence.
ACCESSION   DATLKL010000573 DATLKL010000000
VERSION     DATLKL010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17993"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2081)
                     /locus_tag="VM713_07685"
     CDS             complement(<1..2081)
                     /locus_tag="VM713_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HVH84179.1"
                     /translation="MTLITGCAAERLHREGLAAVEAGDYETGVADLSQALARQPNNNT
                     FRLDLQARRESAVQALIAEADRARLTGQFDGAAALYRRVLALDPGNDRARRGLDNIDA
                     DKRHTTDVQAARKDFEKKDFDAAEAKVRAVLSEDPGFVLAQELASDINGARRPVAAVP
                     HLHPRGNQKVSLQLRDAPTKMVFEVSQRETGINFILDKDVKSDSKTTIFVQDVPVEEA
                     IELVLEQNQLSRNILADNMVMIYPNTAAKQKDYEQQIVRIFYLTNAAPKDVESMLKTV
                     LGAKTLFINEQANMLVMRDTPDAVRMAEKLVTSLDVTEPEVMLEVEVLEISSSRLQDL
                     GIQYPTALTLTPSPLTAAAAGATTGGLVLSDLSHQNSHTIGINSISVTVNAMKQAGAV
                     KTLASPRIRARNKEKAKVLIGTREPVITNSVTPTAAGAPVVTGSVQYLDVGLTLDVQP
                     TVYLDNDVGIKISLEVSSLLKQVTTASGTVAYEIGTRNASTLLRLKDGETQILAGLIQ
                     DSDTRTANTIPLIGEVPILSHLFGTHHTDREKDEIVLSITPRIIRMQPRPAADSTEFW
                     YGSESRLRARPLFSSEPSGAASPAGSAAPPAPLQVPSGSPMPGSGMQPISPSVAPGSV
                     SAPLSGTPQTGEGASLAPAAPPPAPQGLATTTPPAAIAAAEPTAARGAAADAPGPSAL
                     TVTGPAEAKVG"
BASE COUNT          284 a          690 c          759 g          385 t
ORIGIN      
        1 tcccctacct tcgcctccgc gggtcccgtg acggtcagcg ccgagggccc gggcgcgtcg
       61 gcggccgcgc cgcgcgcggc ggtcggctcg gccgccgcga tggctgcggg cggtgtcgtg
      121 gtggcgaggc cctggggcgc cggcggcggc gccgcgggcg caagcgatgc gccttctccc
      181 gtttgcggag tgccgcttaa gggggccgag accgagcccg gggcgactga agggctgatg
      241 ggttgcattc cgctgcctgg catcggacta cccgagggca cctgcagcgg cgccggcggc
      301 gcggccgacc ccgcgggcga tgcagctcct gagggttcgg aggagaagag cggacgcgcg
      361 cgcaggcgcg actcgctgcc gtaccagaat tccgtcgagt cggccgccgg ccgcggctgc
      421 atgcggatga tgcgcggcgt gatcgacagc acgatctcgt ccttctcccg atcggtgtga
      481 tgggtgccga acaggtgcga gagaatcggc acctcgccga tgagcggaat agtgttggcg
      541 gtgcgcgtgt ccgagtcctg gatgagaccg gcgaggatct gcgtctcgcc gtccttgagg
      601 cgcagcagcg tgctcgcgtt gcgcgtgccg atctcgtagg ccacggtccc cgaggcggtc
      661 gtcacctgct tgaggagcga gctcacctcg aggctgatct tgatgccgac gtcgttatcg
      721 aggtagacgg tcggctgcac gtcgagggtg agacccacgt cgaggtactg gacgctccct
      781 gtgaccacgg gggcgccggc ggccgtcggc gtcacgctgt tggtgatcac cggctcgcgt
      841 gtgccgatca gcaccttggc cttctcctta ttgcgcgccc ggatgcgcgg gctcgcgagc
      901 gtcttgacgg cgcccgcctg cttcatggcg ttcaccgtca cgctgatgga gttgatgccg
      961 atcgtatgcg agttctggtg cgagagatcc gagagcacca gtccgccggt cgtggcgccc
     1021 gcagccgcgg cagtgagcgg cgagggggtg agcgtgaggg ccgtcgggta ctggatgccg
     1081 agatcctgca gcctgctgct cgagatctcc agcacctcca cctccagcat cacctcgggc
     1141 tcggtcacgt cgagcgaggt gaccagcttc tccgccatac gcaccgcgtc cggcgtgtcg
     1201 cgcatcacca gcatgttggc ttgctcgttg atgaacagcg tcttggcgcc gagcaccgtc
     1261 ttcagcatcg actcgacgtc cttgggagcg gcgttggtga ggtagaagat ccgcacgatc
     1321 tgctgctcgt agtccttctg cttggcggcg gtgttgggat agatcatcac catgttgtca
     1381 gccaggatgt tgcgcgacag ctggttctgc tcgagcacca gctcgatcgc ctcctcgacc
     1441 ggcacgtcct gcacgaagat ggtggtcttg ctgtcgctct tcacgtcctt gtcgaggatg
     1501 aagttgatgc cggtctcgcg ctgcgacacc tcgaacacca tcttggtggg ggcatcgcgc
     1561 agctgcagcg agaccttttg gttgccgcgc ggatgcaggt gcggcaccgc cgcgaccggc
     1621 cgccgcgcgc cgttgatgtc ggaggccagc tcctgcgcca gcacgaagcc cggatcctcg
     1681 ctgagcaccg cccggacttt ggcttcggcc gcgtcgaagt ccttcttctc gaagtccttg
     1741 cgcgccgcct gcacgtcggt ggtatggcgt ttgtcggcgt cgatattgtc gagcccgcgc
     1801 cgggcccggt cgttgcctgg atcgagcgcc agtacgcgcc ggtagagcgc ggcggcaccg
     1861 tcgaactggc cggtcaggcg ggcgcggtcc gcctcggcga tgagcgcttg caccgccgac
     1921 tcgcgccgcg cctggaggtc caggcggaac gtgttgttat tgggctggcg cgccagcgcc
     1981 tggctcaagt ccgccacgcc ggtctcgtaa tcgccggcct cgacggccgc gaggccctcg
     2041 cgatgcagcc gttcggctgc gcaacctgtg atgagtgtca agacgaacgt caagatgagc
     2101 gatgcgccgc gcgagcgg
//
LOCUS       DATLKL010000574         3871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_209862, whole genome shotgun sequence.
ACCESSION   DATLKL010000574 DATLKL010000000
VERSION     DATLKL010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209862"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VM713_07690"
     CDS             <1..1080
                     /locus_tag="VM713_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVH84180.1"
                     /translation="EFVRNEIAPHYERWERAGVVPREIWKTAGSLGFLCTWADEAYGG
                     AGCRDFRYDQIVAEEMGHTAGVAFGLHSTVVAPYLDRLGTEEQKRRLLPRAVKGEAIL
                     AIAMTEPAAGSDLAGIETHAELRGGSWLLNGSKTFVSNGINADVVIVAAKTDPINPRA
                     IGLFLVESGMPGFDRGRKLEKLGYHAQDTAELFFRDVAISCANVLGDPRRGLEALKSL
                     LVDERLMAACGSLALVHAAFDVTLPYVKERKAFGQRVADFQNTRWKLAEIQTELDVGQ
                     AFVDRCVVDLNAGALTPEAAAEAKLWTAELAGRATDACLQLFGGYGYMWEYPIARLYA
                     AARVHRIWAGTSEIMKEIIGRSLVK"
     gene            1077..2285
                     /locus_tag="VM713_07695"
     CDS             1077..2285
                     /locus_tag="VM713_07695"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HVH84181.1"
                     /translation="MREAFIYDAVRTPRGKGKATGALCQCTPVSLASTVLRAIQQRSG
                     LDTALVDDVILGCVEVTGEQGGDVARSAVLHAGYRETVAGVTVSRFCSSGLEACNMAA
                     AKVICGEAELAIGGGVESMSRVPMGSSGGALFSDPSVAFPAHSVPQGISADLLATLRG
                     FDRRDLDCYALASQHRAAHAWKSGYFQKSIIPVVDLNGETILDRDELIRPHTTMEDLE
                     RLEASFAAMGEKYGYDATAIHRYPEVESIRHLHHAGNSSGIADGAAAVLIGSGEIGAK
                     LGLKPRARFRSFVSIGSEPCIMLTGPAPATRKALAKAGMAASDVGLFEVNEAFAAVVL
                     CFIEEMAVDDERVNVNGGAIAMGHPLGATGAVLLGTLLDEMERRNLESGLVTLCVGGG
                     MGTAALIERV"
     gene            2282..>3871
                     /locus_tag="VM713_07700"
     CDS             2282..>3871
                     /locus_tag="VM713_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HVH84182.1"
                     /translation="MRVLAIGGPEFGRQAEAAMVDAGVIVRISRAESPSGGLLRRIET
                     SGTPFVAVIDGPVGPAAFEIALACHYRVAADDPSIRIVVCDPRAETMPECGATQRLPR
                     LAGVPRALALLLEGKALTVQDAQAAGLLDAVVPADSVIAHAESWIAAQNGRIRQPWDR
                     PGFRIPGLTGAPFGAAVATLRASTLWMYPAPLAILSSVYEGCQVDLDNGLKIEARYAA
                     HVRATAESRNLARLRRFREELGGVPRSEFSRIGILGAGMMGSGIAYSCAAAGLDVVLL
                     DVTREKAERGRAAALGVFEKRGANGRFASIHATCDFADLEECGVVIEAVFEDRAEKAE
                     VTRKTEAVTGSGILFASNTSTLPITGLAEAWTRPENFIGMHFFSPVDRMPLVEIIRGK
                     QTSEASLARALDLGRRVGKTPIVVNDSRGFYTSRVFSTFLNEGMCMLLEGISPALIEN
                     CGRMAGMPGGPLAVADEVSLDLIGRVRLQTQADLGDRYRPNATDEVLRIMIEEQGRHG
                     RKSRQGFYDYRESGRKRLWPGL"
BASE COUNT          640 a         1151 c         1420 g          660 t
ORIGIN      
        1 gaattcgtgc gcaacgaaat cgcaccgcat tacgagcggt gggagcgcgc cggcgtggta
       61 ccgcgggaga tctggaagac tgcaggctca ctcggcttcc tgtgcacctg ggcggacgaa
      121 gcctacggtg gcgcgggctg ccgggatttc cgatacgacc agatcgtcgc cgaagaaatg
      181 ggccacaccg caggcgtggc gttcgggctg cattccacgg tcgtggcacc gtacctggac
      241 cggctcggaa cggaggagca gaagcgccgg ctgcttccgc gagccgtaaa gggcgaggcg
      301 atcctggcga tcgcgatgac cgaacccgca gcgggcagcg atctcgccgg tatcgagacg
      361 cacgcggagc tgcgcggtgg gtcgtggctg ttgaacgggt cgaagacgtt cgtctcaaac
      421 ggcatcaacg cggacgtcgt gatcgtggcg gcaaagaccg atcccatcaa tccgcgcgcc
      481 atcgggctgt tcctggtgga gtccggaatg cccggcttcg accgcggacg gaagctggag
      541 aagctcggat accacgcgca ggatacggcg gagctgttct tccgcgatgt ggcgatctct
      601 tgcgccaacg ttctgggcga tccgcgccgc gggctggagg cgttgaaatc gctgctggtc
      661 gatgagcggc tgatggcggc ctgcgggtcg ctggcgctgg ttcacgcggc cttcgatgtc
      721 acgctgccat acgtcaaaga gcgaaaggca ttcggccagc gtgtggccga cttccagaac
      781 acgcggtgga agctcgccga gatacagacc gagttagatg tcggccaggc gttcgtggac
      841 cgctgcgtgg tggaccttaa cgctggcgcg ctgaccccag aggcggcggc cgaggccaag
      901 ctgtggacgg ccgaactggc cggccgcgcc accgatgcct gcctgcaact gtttggcggc
      961 tacgggtata tgtgggagta tccgatcgcg cgcctgtatg ccgcggcgcg ggtgcaccgc
     1021 atctgggccg gcacgtcgga gatcatgaag gagatcatcg ggaggtcgct ggtgaaatga
     1081 gagaggcctt catttacgat gcggtgcgca cgccgcgggg aaaggggaag gcgaccgggg
     1141 cgctgtgcca gtgtacgccc gtatcgctcg cttcgaccgt tctgcgggcc atccagcagc
     1201 gcagcggact cgacacggcg ctggtcgacg acgtcatcct cggctgcgtg gaagttacgg
     1261 gcgagcaggg tggcgacgtt gcgcgctcgg cggtgctgca cgccggatat cgagagacgg
     1321 tggcgggcgt gacggttagc cgcttctgct catcggggct ggaggcgtgc aacatggccg
     1381 cggccaaggt gatttgcggg gaggcggagc tggcgatcgg cggcggcgtg gagagcatgt
     1441 cgcgcgttcc catggggtca agcggcggcg cgctgttctc agatccgagc gttgctttcc
     1501 cggcgcattc ggtgccgcag gggatttcgg ccgacctatt ggcgaccctg cgcggcttcg
     1561 accgccgcga tctcgactgc tacgcgctgg cgagccagca tcgcgcggcg cacgcctgga
     1621 agagcggcta ttttcagaag tcgatcattc cggtggtcga tttgaacggg gaaacgattc
     1681 tcgatcgtga cgagctgatc cggccccata ccacgatgga ggatctggag cggctggagg
     1741 cctcgttcgc ggcgatgggc gagaagtacg gctacgacgc gacggcgatc catcggtatc
     1801 ctgaagtcga atcgatccgg cacctgcacc acgcgggtaa ctcgtcgggg atcgccgacg
     1861 gcgcggcggc tgtgctgatc ggcagcggcg agatcggcgc gaagctcggg ctcaagccgc
     1921 gggcgcggtt tcgctccttc gtcagcatcg ggagcgagcc gtgcatcatg ctgactggcc
     1981 cagcgccagc gacgcgcaag gcgctcgcca aggccggcat ggccgcgagc gacgtcgggc
     2041 tgttcgaggt gaacgaagcg ttcgcggcgg tggttctgtg cttcatcgag gagatggcgg
     2101 tggacgatga gcgcgtgaac gtgaacggcg gcgcgatcgc gatgggccat ccattgggcg
     2161 cgacgggcgc ggtgctgctg ggaacgctgc tggacgagat ggagcggcgc aatctcgagt
     2221 cggggctcgt gacgctgtgc gtcggtggag ggatgggtac cgcggccctc attgagcgcg
     2281 tatgagagtt ctcgcgatcg gcgggcctga attcgggcgg caggccgaag cggcaatggt
     2341 ggacgccgga gtgatcgtca gaatcagccg tgccgaatct cccagcggcg gtctcctccg
     2401 ccggatcgag acctccggca caccgttcgt ggcggtcatt gacggaccgg ttggcccggc
     2461 ggcattcgag attgctctgg cgtgccacta ccgggtggca gcggacgatc cgtcgattcg
     2521 gatagtggtt tgcgatccgc gcgcggaaac aatgccggag tgcggcgcga cacaacggct
     2581 gccccggttg gccggcgtgc cacgtgcgct cgcgcttctg ctggagggaa aggcgttgac
     2641 cgttcaggac gcgcaggcgg caggcttact ggacgccgtc gtgcctgcgg actccgtgat
     2701 agcgcacgcg gaatcgtgga ttgcggctca gaacggccgg atacgacagc cgtgggaccg
     2761 gccaggtttt cgcatcccgg ggctaaccgg cgcgccgttc ggcgccgccg tcgcgacctt
     2821 gcgggcgagc acgctgtgga tgtaccctgc tccgctcgcg attctctcca gcgtctacga
     2881 aggatgccag gtggacctcg acaacgggtt gaagatcgag gcacggtatg cggcgcacgt
     2941 gcgcgccacg gccgagtcgc gcaatctggc gcggctgcgc cggttccgcg aggaactggg
     3001 cggagttccg cgctcggagt tctccaggat cggcatcctg ggcgctggaa tgatggggtc
     3061 gggaatcgcg tatagctgcg cggcagctgg gctggacgtc gtgctgctgg atgtgacgcg
     3121 cgaaaaggcc gagcgcgggc gtgccgccgc actcggtgtc ttcgagaagc gcggggccaa
     3181 tggccggttt gcgtccattc acgccacctg cgactttgcc gacctcgaag aatgcggggt
     3241 ggtgatcgag gccgtcttcg aggatcgggc cgaaaaagcg gaggtcaccc gcaagaccga
     3301 ggcggtcacg ggcagcggta tcctcttcgc ctcaaatacc tccacgctgc cgattaccgg
     3361 cctcgccgaa gcctggacac ggccggagaa cttcatcggg atgcacttct tctcgccggt
     3421 cgaccgtatg ccgctcgtcg aaatcatccg cggaaagcag acctccgaag cctccctggc
     3481 gcgcgctctc gacctcggtc ggcgggtcgg caaaacaccg atcgtggtga acgacagccg
     3541 agggttctat accagccggg tgttctcgac cttcctgaac gaaggcatgt gcatgctgct
     3601 tgagggcatc tcgccagcgc tcatcgagaa ctgcggccgt atggccggca tgccgggcgg
     3661 tccgctggcg gtagccgatg aggtctcgct cgacctgatc ggccgtgtcc gccttcagac
     3721 ccaggccgac ctgggcgacc ggtatcgccc gaatgcgact gacgaagtgc tgcgcattat
     3781 gatcgaggag cagggccggc atggcaggaa gtcgcggcag gggttctacg attatcgaga
     3841 gagcggccgg aagcggctct ggccggggtt g
//
LOCUS       DATLKL010000575         1698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_209883, whole genome shotgun sequence.
ACCESSION   DATLKL010000575 DATLKL010000000
VERSION     DATLKL010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209883"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1698
                     /locus_tag="VM713_07705"
     CDS             <1..>1698
                     /locus_tag="VM713_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HVH84183.1"
                     /translation="VLLTTGAALEAQIDRLEIHYLASPDGELYFALLSDWADAPTESA
                     AGDEALVVMARAAIARLNRLHGDGPAGARFLLLHRRRVWSDAQRQWMGWERKRGKLHE
                     LNCLLRGATDTTFCGIDGHAPEVPAGVRYVITLDADTRLPRDTARRLVGKMAHPLNRP
                     RFDATSRQVVEGYAVLQPRVAPSLPVGRQGSLFQRVFSSASGIDPYAAAVSDVYQDLF
                     GQGSYAGKGIYDVDAFEAALAGRVADGTLLSHDLFEGLFARAGLVSDIEVVEEFPSRY
                     DVAAARQHRWARGDWQLLPWILGRRDATESNRGGRTLTLIALWKMLDNLRRSLSAPAS
                     IAALLAGWLLSRNTALLWTAFVVLTIGLPALLPIFAALVPHRAGVNTRSHLRALRSDL
                     GLAASLTAFLIVFLAQQAWLMVDAISRTLFRLFVSRRNLLQWVTAAQANLRPQLDLNG
                     AYRQMSGGVAIAMLAAALVGHFRPGAWPVAMPFVVLWVASPAIAVWISLSPLVAGRPA
                     VTDADARALRLIARRTWRYFETFVTADDQMLPPDNFQEDPRPILAHRTSPTNLGLYLL
                     "
BASE COUNT          256 a          583 c          534 g          325 t
ORIGIN      
        1 ggtcttgctc accactggcg ccgcactcga agcacagatc gaccgcctcg agattcacta
       61 tctcgcctcg ccagacggcg agctgtactt tgcgctgctc tcggactggg ccgacgcccc
      121 aaccgagagc gctgccgggg atgaggcgct tgtcgtgatg gcgcgcgccg cgattgcgcg
      181 actgaatcga cttcacggcg acggtccggc gggagcgcgc ttccttctgc tccaccgtcg
      241 gcgtgtctgg agcgatgcgc agaggcagtg gatgggatgg gagcgcaagc gcggcaagct
      301 tcacgaactg aattgccttc tgcgcggagc gaccgacacg accttctgcg gcatcgatgg
      361 gcatgccccc gaggttcccg cgggtgtccg ctatgtcatc acgctcgatg ccgacaccag
      421 gctgccgcgg gacacggcac gccgtctggt cggcaaaatg gcccacccgc tgaatcgtcc
      481 gcgcttcgac gccacgagta gacaggtggt cgagggctac gccgtcctgc aaccgcgggt
      541 cgcgccgtct ttgccggtcg gtcgccaagg ctcgcttttt cagcgcgtct tctcgagcgc
      601 gagcggtatc gacccgtatg cggccgccgt gtcggatgtc tatcaggatc tgtttggtca
      661 gggctcctac gcgggcaagg gcatctacga cgtagacgcg ttcgaagcgg cgctagccgg
      721 tcgcgtggcc gacggcaccc tactcagtca cgatctgttc gagggactct ttgcgcgcgc
      781 ggggctggtt tccgacatcg aggtggtcga ggaattcccg tcgcggtacg acgtcgcggc
      841 cgcacgccag caccgctggg cgcggggcga ttggcaactg ctgccctgga tcctcggcag
      901 gcgcgacgcg acggaaagca atcggggcgg ccgcaccctg acgctgatcg cgctgtggaa
      961 aatgctggac aatctgcggc gctcgttgtc ggcgcccgcg agtatcgcgg ctctgctggc
     1021 cggatggctg ttgtcccgca acaccgctct gctctggacc gccttcgtcg tattgacaat
     1081 tggcctgccg gcgctactgc cgattttcgc cgccctcgta cctcaccgcg ccggcgtcaa
     1141 cacacgcagc cacctgcgcg cgctgcgcag cgacctcggg cttgccgcct cactgaccgc
     1201 cttcttgata gtatttctcg cgcagcaggc gtggctcatg gtcgacgcga tcagtcgcac
     1261 gctgtttcgt ctgttcgtca gtcggcggaa cctgcttcag tgggtcaccg cagctcaagc
     1321 gaacctcagg ccccaacttg atctcaacgg cgcctatcgc caaatgagcg gaggcgtagc
     1381 gatcgcgatg cttgccgcgg cgctcgtcgg acacttccgg cccggcgcct ggcctgtggc
     1441 gatgccgttt gtggtcctct gggtcgcctc ccctgccatc gccgtatgga tcagcctctc
     1501 cccactcgtc gcgggtcgcc cggcggtcac cgacgccgac gcccgcgcac tgagactgat
     1561 cgcccggcga acatggcgtt acttcgagac atttgttact gccgacgacc agatgctgcc
     1621 gcctgataac ttccaggaag atccgcgacc gatcctggcg catcgaacct cccccaccaa
     1681 ccttggcctc tatcttct
//
LOCUS       DATLKL010000576         4480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_187511, whole genome shotgun sequence.
ACCESSION   DATLKL010000576 DATLKL010000000
VERSION     DATLKL010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187511"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VM713_07710"
     CDS             complement(<1..225)
                     /locus_tag="VM713_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84184.1"
                     /translation="MSTLREAAARPPLVMRCGAFGDMVLLTVLLEQLHARFKAPVDVI
                     ASGPWSEPLLSSHRAVGRMFILRSRRTPYWL"
     gene            complement(222..974)
                     /locus_tag="VM713_07715"
     CDS             complement(222..974)
                     /locus_tag="VM713_07715"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HVH84185.1"
                     /translation="MSGGDDGGLRNTLYRTATRSSRRMTRARRALYALAAPLGAALIR
                     AWWRSCRVVRIEGEEHLAAALARAPSLIPCYWHQHQLFCGKFLVEQRARGLTPGWLIS
                     PSVDGELGAMLVRRFGGAVIRGSSSHTGARALRDYYQALTRDAVSPVITPDGPRGPRF
                     KFKPGALLLAQMSQRPILPLAYAASRAWLVKWDKFVIPVPFLARIAIAVGAPRYVARV
                     TDAATLERLEEEMERELQRLYHCARAALGERA"
     gene            complement(971..1420)
                     /locus_tag="VM713_07720"
     CDS             complement(971..1420)
                     /locus_tag="VM713_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84186.1"
                     /translation="MALALRALAHLTGARIAPGELAFPEGGKPRLARAAGDSGEGPDF
                     SISHSGPWVACAAVARGRVGFDVELGSEARLESWAAREAALKACGASLTEARDVELIE
                     GGARCRDMRLHALRLELFPGAAACVMTSLPVHGLEAHALTLAELFAS"
     gene            1578..2894
                     /locus_tag="VM713_07725"
     CDS             1578..2894
                     /locus_tag="VM713_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVH84187.1"
                     /translation="MTAAATAHPLPPEAELQFDVGSTDDSLERMIELFARHGDTYRVF
                     VPARRSYTYVIHHPDDVKRVLVGNHRNYTKGLGLDRVKILLGKGIMTSEGELWKRQRY
                     MMQPLFHRRVITGFAAVIAAANDRFLARWEAVAERGELVNLTDEMSELTLEIVLRSIF
                     GRDLDRLTQQLGGNPFEVVTKEQSRDLQFAYKFRSLTKLVAQLIERRRAESADEHFDY
                     IAMLMSARDKETGAPMSERELVDEVMTLIVAGHETTASGLNWTWYLLAQHPEAEARLH
                     AELDAAPQLSAPGLAEMETLGFTAQVVNEALRLYPPGWLLSRRAVAADVLGGFAVPAG
                     TNVLLPLYLLHRHPRYWQEPDRFWPERFAPEREAERPRFAYMPFAAGPRHCIGETFAL
                     YEMLLHLYKVARRYRLVYVPDKPHELEAQINLRTRHPLHMRLEARR"
     gene            2891..>4480
                     /locus_tag="VM713_07730"
     CDS             2891..>4480
                     /locus_tag="VM713_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVH84188.1"
                     /translation="MIKATTLTEVLEANRAFPRSITYLEGESESRSVSAQELYERALG
                     ILYHLQRIGARRGDKLILFLGNNEQFIDVFWAAILGGIVPVPVALGISDEHRHKVLRI
                     ARRLGSPFLYTERRSLERIGAFAAGAGEQELFERLRARTFLVDDLDDISRAGSIQRAA
                     PDDIAFIQFSSGSTSEPKGVVLTHGNIIANCRGATEAAGFNEDDVSLSWMPLTHDMGL
                     IGFHLVMFANRVHAHLMPTEHFIRRPLLWLTLASRVRATILCSPNFGYKHYLKVLGER
                     PVEGLDLSAVRLIFNGAEPISVELCHEFLTRLGPARLDRNSMYPVYGLAEASLAVSFP
                     PVGAPLKTLTLNRHRLNPDDPVEPVAADDPNALRLVSEGKAIPYTRVRIAGEDDGELP
                     EDRIGHVHMTGENVTRGYYEDPEANAAAFTADGWLRTGDLGLIHAGELYISGRAKEII
                     FVNGQNYYPHDLEGIAQSAPGLELGKVVAAGVRRRGAETEQLVVFVLHRGAVADFLAV
                     ATRLSRLINEQTGLEVAEVIPV"
BASE COUNT          718 a         1595 c         1505 g          662 t
ORIGIN      
        1 cagccagtac ggtgtgcggc ggctgcgcag gatgaacatc cgcccgaccg cgcgatggga
       61 gctcagcagc ggctcggacc agggaccgga ggcgatgaca tccaccggcg ccttaaagcg
      121 cgcgtgcagc tgctcgagga gcaccgtgag cagcaccatg tcgccgaacg cgccgcagcg
      181 catgacgagc ggcggccgcg ccgcggcctc gcgcagcgtg ctcacgcacg ctccccgagt
      241 gcggcccgcg cgcagtggta gaggcgctgc agctcgcgct ccatctcctc ctcgaggcgc
      301 tcgagcgtgg cggcatcggt cacgcgcgcc acatagcgtg gcgccccaac cgcgatggcg
      361 atgcgcgcca gaaacggcac cgggatgacg aacttgtccc acttcaccag ccaggcccgc
      421 gaggccgcat acgccagcgg caggatcggg cgctgcgaca tctgcgcgag cagcagcgcg
      481 cccggcttga acttgaagcg cggcccgcgc ggaccgtccg gcgtgatgac gggcgagacg
      541 gcatcccggg tgagcgcctg atagtaatcg cgcagcgcgc gcgcgccggt gtggctagag
      601 gagccacgga tcaccgcgcc gccgaagcgc cgcaccagca tcgcgccgag ttcgccgtca
      661 accgacggac tgatgagcca acccggggtc agtccgcgcg cgcgctgctc gacgagaaac
      721 tttccgcaga agagctggtg ctggtgccag tagcacggga tcagcgaggg tgcacgcgcg
      781 agtgccgccg ccaggtgttc ctcgccctcg atgcgcacca cgcggcagct gcgccaccag
      841 gcgcgaatga gcgccgcgcc gagcggagcg gcgagcgcgt acagggcgcg gcgcgcgcga
      901 gtcatgcgcc ggctcgagcg cgtcgcggtc cgatacagcg tgttgcgcaa acctccgtcg
      961 tcgccgccgc tcatgaggcg aacagctccg caagagtgag tgcgtgcgct tcgaggccgt
     1021 gcaccgggag gctcgtcatg acgcaggccg cggcgccggg gaacagctcg agccgcaacg
     1081 catgcagccg catgtcgcga cagcgggcac cgccctcgat gagctcgacg tcgcgcgcct
     1141 ccgtgaggct ggcgccgcac gccttgagcg cggcctcgcg cgctgcccag gactcgagcc
     1201 gcgcctcgct gccgagctcg acgtcgaagc caacgcgtcc gcgtgcgacc gccgcgcagg
     1261 ccacccaggg gccggagtga ctgatgctga agtcgggccc ctcaccggag tcgcccgccg
     1321 cgcgtgcgag ccgcggcttg ccgccctcgg gaaaggcgag ctcgcccggg gcgatccgtg
     1381 cgcccgtcaa atgcgcgaga gcgcgcagag cgagcgccac acccgcgagc gtggccggtg
     1441 cgcgcgcggc atgcgcggcg acttcgagac gcttcgcata cggcagccgc ccggcgagcg
     1501 cgcgcgctgc ctcttgcggc cactgaccgc gcaggtccga gtgatataag attaaggttt
     1561 ggctttcaac agcttgcatg accgctgccg cgaccgcgca ccccctgcct cccgaagctg
     1621 agctgcaatt cgacgtcggc tccaccgacg actcgctgga gcggatgatc gaattgtttg
     1681 cccgccacgg tgacacttat cgcgtgttcg tgccggcacg ccgatcttat acctatgtca
     1741 tccatcatcc cgacgacgtg aagcgggtgc tggtcgggaa tcaccgcaac tataccaagg
     1801 gtctcggtct cgaccgcgtc aagatcctcc tcggcaaggg catcatgacg agcgaagggg
     1861 agctgtggaa gcggcagcgc tacatgatgc agccgctgtt ccaccgtcgc gtgatcaccg
     1921 ggttcgccgc ggtgatcgcc gcggccaatg atcgcttcct cgcccgctgg gaggctgtgg
     1981 cggaacgcgg cgagctcgtg aacctcaccg acgagatgag cgagctcacc ctcgagatcg
     2041 tgctgcgctc gatcttcggc cgcgacctcg accgcctcac gcagcagctc ggcggtaacc
     2101 ccttcgaggt ggtcaccaag gagcagagcc gcgacctgca gtttgcctac aagttccgct
     2161 ccctcaccaa gctcgtcgcg cagctcatcg agcggcggcg tgccgagagt gcggacgagc
     2221 atttcgacta catcgcgatg ctgatgagtg cgcgcgacaa ggagacggga gctccgatga
     2281 gcgagcgcga gctcgtcgac gaagtgatga cactcatcgt ggccgggcat gagaccacgg
     2341 cgagcgggct caactggacc tggtacctcc tcgcgcagca ccccgaggcg gaggcgcgtc
     2401 tgcatgccga gctcgatgcg gccccgcagc tctcggcccc gggcctcgcc gagatggaga
     2461 cgctcgggtt taccgcgcag gtcgtgaacg aggcgctgcg gctctacccg cctggctggc
     2521 tcctgtcgcg gcgcgcggtc gccgccgacg tgcttggtgg cttcgcggtt ccggccggca
     2581 ccaacgtgct gctgccgctg tacctgctgc accgccaccc gcgctactgg caggagcctg
     2641 accgcttctg gcccgagcgc tttgcacccg agcgcgaggc tgagcgaccg cgcttcgcct
     2701 acatgccctt cgccgccggc ccgcggcact gcatcggcga gacctttgcg ctctacgaga
     2761 tgctgctgca cctctacaag gtggcacgcc gctaccgcct ggtgtacgtg cccgacaagc
     2821 cacacgagct cgaggcacag atcaatcttc gcacccgcca tccgctccac atgaggctgg
     2881 aagcccgccg atgatcaagg ccacgacgtt gaccgaagtg ctcgaggcga accgcgcctt
     2941 cccgcgcagc atcacctacc tggagggcga gagcgagtcg cgcagcgtat cggcgcagga
     3001 gctgtacgag cgcgcgctcg gcatcctcta tcacctgcag cgcatcggcg cgcgccgcgg
     3061 cgacaagctg atcctgttcc tcggcaacaa cgagcagttc atcgacgtgt tctgggcggc
     3121 gatcctcggc ggcatcgtgc cggtgccggt ggcgctcggc atcagcgacg agcaccgcca
     3181 caaggtgctg cggattgcgc gccggctcgg gtcaccgttc ctgtacaccg agcgccgctc
     3241 gctcgagcgc atcggtgcct tcgcagccgg tgccggcgag caggagctgt tcgagcggct
     3301 gcgcgcgcgt accttcctgg tcgatgatct cgatgacatc tcgcgcgccg gcagcatcca
     3361 gcgcgccgcg cccgacgaca tcgccttcat tcagttctcc tccggctcca ccagcgagcc
     3421 gaagggcgtg gtgctcaccc acggcaacat cattgccaac tgccgcggcg cgaccgaggc
     3481 ggccggcttc aacgaggacg acgtgagtct ctcgtggatg ccgctcaccc acgacatggg
     3541 tctcatcggt ttccacctcg tcatgttcgc gaaccgggtg cacgcgcacc tcatgccgac
     3601 ggagcacttt atccgccggc cgctgttatg gctcaccctg gcctcgcgcg tgcgcgcgac
     3661 gatcctgtgc tcgccgaact tcggctacaa gcactacctc aaggtgctcg gcgagcggcc
     3721 ggtcgagggc ctggatctct cggcggtgcg cctcatcttc aacggcgcgg agccgatctc
     3781 ggtcgagctg tgccatgagt tcctcacgcg cctcgggccg gcgcgcctcg accgcaattc
     3841 catgtacccg gtgtacgggc tcgcggaggc ctcgctcgcg gtgagcttcc cgccggttgg
     3901 cgcgccgctc aagaccctga cgctgaaccg ccaccgcctg aaccccgacg acccggtgga
     3961 gccggtcgcg gccgatgacc cgaatgcact gcgcctggtg agcgagggca aggcgatccc
     4021 ctacacccgc gtgcgcatcg cgggcgagga tgacggcgag ctccccgagg atcgcatcgg
     4081 ccacgtgcac atgacgggtg agaatgtgac gcgcggctac tacgaggacc cggaggccaa
     4141 cgccgcggcc ttcacggccg acggctggct gcgcaccggc gacttggggc tcatacacgc
     4201 gggcgagctc tacatctcgg gccgcgccaa ggagatcatc ttcgtcaacg gccagaacta
     4261 ctacccgcac gatctcgagg gcatagccca gagcgcgccg ggcctcgagc tcggcaaggt
     4321 cgtcgcggcg ggcgtacgcc gccggggcgc ggagaccgag caactggtgg tgttcgtcct
     4381 gcatcgcggc gccgtcgcgg acttcttggc cgtagcgacc cggctgtcgc ggctcatcaa
     4441 cgagcagacc gggctcgagg tcgccgaggt cattccggtc
//
LOCUS       DATLKL010000577         1753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_164830, whole genome shotgun sequence.
ACCESSION   DATLKL010000577 DATLKL010000000
VERSION     DATLKL010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164830"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VM713_07735"
     CDS             complement(<1..292)
                     /locus_tag="VM713_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVH84189.1"
                     /translation="MAEGEEAFRLDRRGARAAFDRASAGYEAAARLQARVSTELLERL
                     AALELAPRVVLDLGAGTGRVTRELKRRYPGALVIALDLAPGMLREAQRNQMLW"
     gene            complement(285..1073)
                     /locus_tag="VM713_07740"
     CDS             complement(285..1073)
                     /locus_tag="VM713_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVH84190.1"
                     /translation="MSALHAAVSGRGRRDLVMLHGWGMNLEVWSELAEALARRFRVIA
                     VDLPGHGRSDWDGAAYTPAAQTFRVHETLAPLTTRYHLLGWSLGGQFALDLAAAMPAG
                     IERLILVATTPRFLAGPGWRCGVKQASLERLAGELHADPEKAVRGFLKSQVRGLASRT
                     AGRVLERLRGALTHGGGAQPEALAWGLERLRQADLRPQLAQVRAPALVVAGEDDRITP
                     PAASRALAKALPRGHFRQLRGAGHVPFLSHRDQFLAALDGFLRG"
     gene            complement(1070..>1753)
                     /locus_tag="VM713_07745"
     CDS             complement(1070..>1753)
                     /locus_tag="VM713_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH84191.1"
                     /translation="RTALIGTDGVFSMDGDLAPLPALALAARRANAWLVVDDAHGLGV
                     VGASGRGTLEESGVGVAEVPVLVGTLGKAFGSFGAFVAGEAALIDYLMQRARSYIYTT
                     ALPQPVAAASRKALELIQREDWRRTRLAALVGRFRRLALEARVPLGLSRTPIQPVVLG
                     TETAVLAAQRALEAEGLLVVAIRPPTVPKGSARLRVTLTAAHTEGEIDRLAEALGRCC
                     ARLSATGAA"
BASE COUNT          244 a          657 c          656 g          196 t
ORIGIN      
        1 gccacagcat ctggttgcgc tgcgcctcgc gcagcatgcc gggcgcgagg tcgagcgcga
       61 tcaccagcgc acccgggtag cggcgcttga gctcgcgcgt cacgcggccc gtgccggcac
      121 ctaagtcgag caccacgcgc ggcgcgagct cgagggcggc gagccgctcg aggagctccg
      181 tcgacacccg cgcctggagc cgcgcggcgg cctcgtaacc ggcgctcgcc cggtcgaacg
      241 ccgcccgcgc accacggcga tcgagccgga aggcttcctc gccctcagcc acgcaggaac
      301 ccatcgagcg cggcgaggaa ctggtcgcga tgcgagagga agggcacatg acccgcgccg
      361 cgcagctgac ggaaatgccc cctggggagc gccttggcga gcgcccggga agccgccggg
      421 ggcgtgatgc gatcgtcctc cccggcgacg accagagcag gcgcgcgcac ctgcgccagc
      481 tggggccgca gatccgcctg ccgcagccgc tcgaggcccc aggcgagcgc ctcaggttgc
      541 gcgccgcctc cgtgcgtcag cgcgccgcgc agccgctcga gcacccggcc cgcggtccgt
      601 gaggcgaggc cgcgcacctg cgacttgagg aatccgcgca ccgccttctc aggatctgcg
      661 tgcaactcgc cggcgagccg ctcgagggat gcctgcttca ccccgcagcg ccagccgggg
      721 cccgcgagaa accgcggcgt ggtcgcgacg aggatgagcc gctcgatgcc ggcgggcatc
      781 gcggccgcga gatcgagcgc gaactggccg ccgagcgacc agccgagcag gtgatagcgg
      841 gtcgtgaggg gagccagcgt ctcgtgcacg cggaaggtct gcgccgcggg cgtgtacgcc
      901 gcgccgtccc agtcgctgcg cccgtggccg ggcagatcga cggcgatcac ccggaagcgc
      961 cgcgcgagcg cctccgcgag ctcgctccat acctcgaggt tcattcccca gccgtgcagc
     1021 atcaccaggt cgcgccgccc gcgcccgctg accgcggcgt gcagcgcgct catgcggccc
     1081 ccgttgcgct gagccgcgca cagcagcggc cgagcgcctc ggcgagccga tcaatctcac
     1141 cctcggtgtg cgccgccgtg agcgtgacgc gcagccttgc tgagcccttc ggtacggtcg
     1201 gcgggcggat ggcgacgacg agcaggccct ccgcctcgag cgcgcgctgc gcggcgagca
     1261 ccgccgtctc ggttccgagc acgacgggct gaatgggggt gcgcgagagg ccgagcggca
     1321 cgcgcgcctc gagggcgagg cggcggaagc gcccgaccag cgccgcgagg cgggtgcgcc
     1381 gccagtcctc tctctggatg agctcgagtg ccttgcggct ggccgccgcg accggctgcg
     1441 ggagcgcggt cgtgtagata taggagcgtg ccctctgcat gaggtagtcg atgagggcgg
     1501 cctcgccggc cacgaaggcg ccgaaggacc cgaaggcctt gccgagcgtg ccgacgagca
     1561 cggggacctc cgcgacgccg acgccgctct cctccagcgt gccgcgcccg ctcgcgccga
     1621 cgaccccgag gccgtgcgca tcgtcgacga cgagccaggc gttggcgcgg cgcgccgcga
     1681 gcgcgagcgc cgggagcggc gcgagatcgc cgtccatgct gaagacgccg tcggtgccga
     1741 tgagcgccgt gcg
//
LOCUS       DATLKL010000578         1636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_187554, whole genome shotgun sequence.
ACCESSION   DATLKL010000578 DATLKL010000000
VERSION     DATLKL010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187554"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..>1636)
                     /locus_tag="VM713_07750"
     CDS             complement(139..>1636)
                     /locus_tag="VM713_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH84192.1"
                     /translation="EGSQDASFQAMARNQQQVAAVVREDSNVEMYMSFVGASGPRPTV
                     NSGTLFLRLKDRKLRKLTADEVVQELRAKLSGFPGVKVYLQNPPSISVGGQLTKAQYQ
                     YTLQDADLNELYATAERFVGALRQVPGFLDVNTDMQISSPQLAVDINRDKATALGVTA
                     QQIETALGLAFSSQQISTIYTSLDQYQVILELLPQYQDDPNSLSRLYIRSTNGSLVRL
                     DAVAKITRGVGPLTINHLGQLPSVTVSFNLAPDTSLGAAVQRIQQLERQIKMPAGVST
                     SFQGTAQAFQSSLQGLGILLLMAVVVVYIVLGVLYESFIHPLTILSGLPSAAVGALIT
                     LMFFDVTLSLYAFVGVIMLIGIVKKNAIMMIDFALARQRGEGIGPAEAIVEAALIRFR
                     PIMMTTMAALMGTLPIALGVGQGSEARRPLGLAVVGGLMLSQLLTLYLTPVIYVYLDR
                     LQGARFILRRVRPARPPVAPAPAGKEAGGVQPFPARERKASEPRKAAR"
BASE COUNT          281 a          492 c          586 g          277 t
ORIGIN      
        1 tgtggaccgt ctgcaccggt tcggcggcgc gggccggcag cgcgagtgcg ccgaccagcg
       61 tgaggacgag cgctgcggcc tcgcctgggt tcctcagcat gaatggtctc cctcgttgcg
      121 gtcggattgg cgtgcgggct aacgggccgc cttgcgcggc tcggacgcct tgcgctcgcg
      181 ggccgggaag ggctggactc cgccggcctc cttgcccgct ggcgctggcg ccacgggcgg
      241 acgcgccggc ctgacgcgcc gcagaatgaa tcgcgcgccc tgcagcctgt cgagatagac
      301 gtaaatcacc ggggtgagat agagcgtcag caattgcgag agcatgagcc cgcccaccac
      361 ggcgaggccg agcgggcgac gcgcctccga gccttggccc acgccgagcg cgatcggcag
      421 cgtgcccatg agggccgcca tggtcgtcat catgatcggg cggaaacgga tgagggcggc
      481 ctcgacgatc gcctcggcag gccctatgcc ttcgccacgc tgccgcgcca gcgcgaagtc
      541 gatcatcatg atcgcgtttt tcttgacgat gccgatcagc atgatgacgc cgacgaaggc
      601 gtaaaggctg agcgtcacgt cgaagaacat gagcgtgatg agcgcgccca ccgcggccga
      661 aggcaggcca gagaggatcg tcagcggatg gatgaagctc tcgtagagta cgccgagcac
      721 gatgtagacg actacgacgg ccatgagcag caggatgccg agcccctgga gcgaggactg
      781 gaaggcttgc gccgtcccct gaaagctggt gctgacgccg gccggcatct tgatctggcg
      841 ctcgagctgc tgaatgcgct gcaccgccgc gccgagcgag gtatcgggcg ccaggttgaa
      901 ggagacggtc accgagggca gctggccgag atggttgatg gtcagcgggc ctactccgcg
      961 cgtgatcttg gcgaccgcgt cgagtcgcac gagcgaccca ttggtcgatc ggatatagag
     1021 acgcgagaga gaattggggt cgtcctggta ctgaggcagg agctcgagaa tcacctggta
     1081 ttggtcgagc gaggtgtaga tggtcgagat ctgctgagag ctgaaggcga gcccgagtgc
     1141 ggtctcgatc tgctgcgccg taacgccgag ggcggtcgcc ttgtcacggt tgatatcgac
     1201 cgccagctgc gggctcgaga tctgcatgtc ggtgttgaca tcgagaaagc ccgggacttg
     1261 gcggagagcc ccgacgaacc gttccgccgt cgcgtagagc tcgttgagat cggcgtcctg
     1321 gagtgtgtac tggtactgcg ccttggtgag ctggccgcca acgctgatgc tcggcgggtt
     1381 ctgcaggtag accttgacgc cgggaaagcc ggagagcttg gcgcgcagct cctggaccac
     1441 ctcgtcggcg gtcagcttgc gcagctttcg gtccttcagc ctgaggaaga gcgtgcccga
     1501 attgacggtc gggcgcggtc cgctggcgcc gacgaacgac atgtacattt cgacattcga
     1561 atcttcgcgg acgaccgcgg cgacctgttg ctggttgcgc gccatggcct ggaacgaggc
     1621 gtcctgcgaa ccttcc
//
LOCUS       DATLKL010000579         2970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_119834, whole genome shotgun sequence.
ACCESSION   DATLKL010000579 DATLKL010000000
VERSION     DATLKL010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119834"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /gene="pstB"
                     /locus_tag="VM713_07755"
     CDS             <1..700
                     /gene="pstB"
                     /locus_tag="VM713_07755"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005171697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HVH84193.1"
                     /translation="KNVTIDVSRRQVLAMIGPSGCGKSTFLRCLNRMNDTIPGARVTG
                     SITLDGRDIHDERRDVVQLRARVGMVFQKPNPFPKSIYDNVAYGPRIHGLARSRAHLD
                     DLVCTSLTRAGLWDEVKDRLGGPGTGLSGGQQQRLCIARTIAVQPEVILMDEPCSALD
                     PVATARIEDLIDELRESYAIVIVTHSMQQAARVSQRTAYFHLGELIEVGDTDRVFTNP
                     RHRLTEDYITGRFG"
     gene            complement(770..2173)
                     /locus_tag="VM713_07760"
     CDS             complement(770..2173)
                     /locus_tag="VM713_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HVH84194.1"
                     /translation="MATAGVAQAQTAPAAPAPATKAEDSSLTWKGITLYGIVDIGIQN
                     DTHSAPFSDYFPPGSASLVQKNNYDSPTGVTPSNLSQSRIGISGNEPLVGDWAGVFRF
                     ETFFNPWSGDISDACKSLTLNNGKALDVQTTGVDSSICGQLFAGAAYAGFSSPSYGSF
                     TFGRHVTLLADGIGKYDPLGASNAFSLIGYSGTAAGGGDTEDRRLDQSIKYYAKYDWL
                     HLGALYQFGSASGSINTGYQFMIGGEYAGLSIDGYYFKKYDAISVSALSAAQVTTLQT
                     GTCTAAAPATAQCWPISNSLSATVSDNETYAVMASYAVPGSPVKISGGYERIKFDNPT
                     NPLHAGSLTIGGYILAFTNVQTGPTATFANEKTLDVYWGGVKWGMTPDLDLLAAYYGY
                     HQNSYATGGNAGCSDTRAGNCSGTEAVMSLVFDYRLSKRFDVYAGSMYSGVRDGLANG
                     YLFKTNIATTAGLRFRF"
     gene            2587..>2970
                     /locus_tag="VM713_07765"
     CDS             2587..>2970
                     /locus_tag="VM713_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH84195.1"
                     /translation="MRLLLVEDEKKVSELVARALRQESYAVDVADDGTQGWELAQAYE
                     YDLIILDLMLPGLPGEELLRRIRRGNQQVPILILTARDATEAKVQNFEAGADDYLTKP
                     FAFAELVMRVKALLRRGPVARSSVLR"
BASE COUNT          525 a          944 c          961 g          540 t
ORIGIN      
        1 gaagaatgtc acgatcgacg tgagcaggcg tcaggtgctg gcgatgatcg gaccctcggg
       61 gtgcggcaag tcgacctttc tgcgctgcct caatcgcatg aacgacacca tcccgggtgc
      121 gcgcgttacc ggcagtatca ccctcgacgg ccgcgacatt cacgacgagc gccgcgacgt
      181 ggtgcagctg cgggcccgtg tcggcatggt gttccagaag ccgaacccct ttccgaagtc
      241 gatctacgac aacgtcgcct acgggccgcg cattcatggc ctggcgagga gccgggcaca
      301 tcttgacgac ctcgtctgca ccagcctgac acgcgcgggc ctctgggacg aggtgaagga
      361 ccgactcggc ggccctggta ccgggctctc cggcggccag cagcagcgcc tctgtatcgc
      421 gcgcacgatc gcggtgcagc cggaggtgat tctcatggac gagccctgct cggcgctcga
      481 tcccgtggcg acggcccgca tcgaggatct catcgatgag ctgcgcgagt cctatgccat
      541 cgtgatcgtc acgcactcca tgcagcaggc cgcgcgcgtc tcgcagcgca ccgcctactt
      601 ccatctcggc gagctcatcg aggtcggcga caccgaccgg gtgttcacca acccgcgcca
      661 ccggctcacc gaggactaca tcaccgggcg attcggctag aaacgaaagg gcccgcttgc
      721 gcgggcccct cgattcctcc gccgcggggc ggaagcaagc tgcaacgcct cagaacctga
      781 agcgcagacc cgccgtggtg gcgatgttgg tcttgaagag atagccattg gccaagccgt
      841 ccctgacgcc ggaatacatg ctgcccgcgt agacgtcgaa gcgcttcgac agccggtaat
      901 cgaagaccag ggacatcacc gcctcggtgc cgctgcaatt gccggctctc gtgtcgctgc
      961 agccggcgtt cccgcccgtt gcgtagctat tctggtggta gccatagtac gccgccagca
     1021 ggtcgaggtc cggagtcatg ccccacttca cgccacccca gtagacatcc agagttttct
     1081 cgttggcgaa agtggcggtc gggccggttt gaacgttggt gaaagcgagg atgtagccgc
     1141 cgatcgtcag cgaccccgca tgcagcggat tggtcgggtt gtcgaacttg atgcgctcgt
     1201 agcctcccga gatcttgacc ggcgagcccg gcacggcata cgaggccatc accgcatagg
     1261 tctcgttgtc cgagaccgta gccgagagtg agttgctgat gggccagcac tgcgccgtcg
     1321 ccggcgcggc cgcggtgcag gtgccggtct gcagagtcgt gacctgcgcc gcgctcagag
     1381 cggagacaga gatcgcgtcg tacttcttga agtagtaccc gtcgatcgag aggcccgcgt
     1441 actcgccacc gatcatgaac tggtagccgg tgttgatgct gccgctcgcg ctgccgaact
     1501 ggtacagggc gccgaggtgc agccagtcgt acttggcgta gtacttgatc gactgatcga
     1561 ggcgccggtc ctcggtgtcg ccgccgccgg cggccgtgcc ggagtagccg atcagcgaga
     1621 acgcgttcga cgcacccagg gggtcatatt tgccgatgcc gtcggcgagc agtgtcacgt
     1681 gacggccgaa ggtgaagctg ccgtagctcg gggagctgaa gccggcgtag gcggcgccgg
     1741 cgaacaactg gccgcaaatg ctgctgtcga cgccggtggt ctgcacgtca agcgccttgc
     1801 cgttgttcag cgtcagggac ttgcaggcgt cggagatgtc gcccgaccag gggttgaaga
     1861 acgtctcgaa gcggaacacc ccggcccagt cacccacgag cggctcgttg cccgagatgc
     1921 cgatgcgcga ctgagagagg ttgctcgggg tgacgccggt gggggagtca tagttattct
     1981 tctgcaccag ggaagcggat cccggcggga agtaatcgct gaacggcgcg ctgtgcgtgt
     2041 cgttctgaat gccgatatcg acgatgccgt agagggtgat ccccttccag gtgaggctgc
     2101 tgtcctcagc cttggtcgcc ggtgccggcg cggccggggc ggtctgcgct tgcgctacgc
     2161 ccgctgtcgc caacgaagcc gttaagagac aggcacgaat gggtgtcatc ttcggaattc
     2221 ctgtaagttg aaaaagccga cgcggcggcc agacgcctgg agggtcggac gtctgtgttg
     2281 catcgccgca acctctcacg atcagcccca tccagggctc actccggttc agttgtacga
     2341 catttgtacc gcagtcagat ggcacttttg ttaccgggcc gagattcctg ggcacctaac
     2401 gtcccttccg accgcgagcc tacctgctga gtaacactcc ccgacgggcg ctaaactctt
     2461 ccatatcaaa gccttaaatg caacttctat ttttctgaaa gctttttaat ggggcgtttg
     2521 ccgagcatta atgcccgtat gctccacttc agctgcccga ggccgcaata aggacaagtc
     2581 gcagccatgc gattgctcct cgtcgaggat gagaagaagg tatccgagct cgtggcgcgc
     2641 gcgctgcgcc aggaaagcta cgccgtcgac gtcgccgatg acggcacgca gggctgggag
     2701 ctcgcccagg cctacgagta cgacctcatc attctcgatc tgatgctgcc cgggctcccg
     2761 ggcgaggagc tccttaggcg catccggcgc ggcaaccagc aggtgccgat cctgattctc
     2821 accgcgcgcg atgcgaccga ggcgaaggtc cagaacttcg aggcgggcgc cgacgactat
     2881 ctgacgaagc ccttcgcctt cgccgagctc gtcatgcgcg tcaaggcgct gctgcggcgc
     2941 ggtccggtcg ctcgctcgag cgtgctgcgg
//
LOCUS       DATLKL010000580         3617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_40808, whole genome shotgun sequence.
ACCESSION   DATLKL010000580 DATLKL010000000
VERSION     DATLKL010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40808"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VM713_07770"
     CDS             <1..706
                     /locus_tag="VM713_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HVH84196.1"
                     /translation="RGSTSARVVTFGLTRSADFCASEVSASVAAEGFTTSFRLTAPMG
                     AATIRLGLGGTHNVANALAAAAAATAAGARLEHIVRGLAAVRAVPGRLQFRRAIGGGW
                     LIDDSYNANPSSVRAAIEVLATLPGRRWLVLGDMAELGAFALEEHVRLGEFARSAGLE
                     RLYATGPLSTRAALAFGAGGESFADVPALTKALRAALCGAADDVRVLVKGSRFNRLER
                     VVEAVAGDALPDRGGH"
     gene            707..1789
                     /gene="mraY"
                     /locus_tag="VM713_07775"
     CDS             707..1789
                     /gene="mraY"
                     /locus_tag="VM713_07775"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HVH84197.1"
                     /translation="MLYWLTQQFGHVPGAHVFAYLTFRAILATVSALGLSLVIGPPLI
                     ARLVRGQIGQVVRDDGPPTHLLKAGTPTMGGTLILITTLVSTLLWAELSNRLVWTVLA
                     VTFAFGLIGFYDDYLKLVVRNPRGLVPRWKYFWQSVAGLAAAATLYYTATEPAETTLF
                     LPFFKTFHTPLTAFGFITLAYLMIVGMSNAVNLTDGLDGLAIMPAALVTSALGIFAYA
                     SGNAVFAHYLQIPEIRGAGELLVFCASLAGAGLGFLWFNSYPAQVFMGDVGALAIGAA
                     IGVLAVIVRQELVVLWMGGVFVLETASVILQVASFKLTGRRIFRMAPIHHHFELKGWA
                     EPKVIVRFWIISLLLVLSGLATLKLR"
     gene            1786..3126
                     /gene="murD"
                     /locus_tag="VM713_07780"
     CDS             1786..3126
                     /gene="murD"
                     /locus_tag="VM713_07780"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HVH84198.1"
                     /translation="MNGPTDKRPYAVIVGLGRTGLSAARYLRAQGWRLALTDTREQPP
                     ELAALTALDAGIPVRLGRLDAALLEGALCVVASPGVSLAQPFFAAARARGLEIVGDVE
                     LFARAVAAPVAGVTGTNGKSTVTTLLARMAERSGLRVRAGGNLGEPALDLLGSGAALY
                     VLELSSFQLETATHLACRAATVLNVTADHLDRYASLAEYAAAKARIFVRCDTAVVNLD
                     DPLVMAMPRTARRTLSFSLRAAVGADYEVALHHGGWWLMRRAAPLLPVARLRIKGLHN
                     AANALAALALGEALELPLEPMLRELEEFPGLPHRSQWVAEVRGVTYINDSKGTNVGAT
                     LAAVGGMTGPLVMIAGGDGKNQDFAPLAAAFRGKVRHAVLIGRDAPLLARALAGVCTL
                     ETCATLPEAVRAAARAARPGDTVLLSPACASLDMFRDYTQRGAAFAAAVGELAA"
     gene            3123..>3617
                     /locus_tag="VM713_07785"
     CDS             3123..>3617
                     /locus_tag="VM713_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HVH84199.1"
                     /translation="MSTVMHEPLMGFARSAGRAGGVRLDSVTVAVTLALTLLGLVMVT
                     SASVSVASRETGAPFYYLERQLALVGVGTACAALLFSLRTEWLERASGPLLAVALALL
                     VLVLVPGLGHAANGSRRWLRLAALNFQVSELARVLVLVYVASYAVRREAQLREGLAGL
                     AKPLA"
BASE COUNT          426 a         1315 c         1274 g          602 t
ORIGIN      
        1 gcgcggctcg acgagcgcac gtgtcgtcac cttcggcttg accaggtccg cggacttctg
       61 cgcgagcgag gtctcagcct cggtcgcagc ggagggcttc accacgagct tccgcctcac
      121 cgcgccgatg ggggcggcaa cgatccgtct ggggctgggg ggaacccaca acgtcgccaa
      181 cgccctcgcc gccgcggccg ccgcgaccgc ggcgggtgcc aggctcgagc acatcgtccg
      241 cgggctcgcg gcggtgcgcg ccgttcccgg ccggctgcaa ttccggcgcg ccatcggcgg
      301 cggctggctc atcgacgatt cctacaacgc gaatccgagc tcggtgcgcg ccgccatcga
      361 ggtgctcgcc acgctccccg gccgccgctg gctggtgctc ggtgacatgg ccgagctcgg
      421 cgccttcgct ctcgaggagc acgtgcggct cggcgagttc gcgcgcagcg cggggctcga
      481 gcggctctat gcgacgggcc cgctctcgac gcgcgcggcg cttgccttcg gcgccggcgg
      541 cgagtccttc gccgacgtgc cggcgctcac caaggcgctg cgcgccgccc tctgtggtgc
      601 cgcggacgac gtacgcgtgc tcgtcaaggg ctcgcgcttc aatcgcctcg agcgcgtggt
      661 cgaggccgtg gcgggcgatg cgcttcccga cagaggcgga cactgaatgc tctactggct
      721 cacccagcag tttggtcacg tccccggcgc gcacgtcttc gcctacctga ccttccgcgc
      781 gattctcgcc acggtctcag ctctcgggct gtcgctcgtg atcgggccgc cgctgatcgc
      841 gcggctggta cgcggccaga tcgggcaggt ggtgcgcgac gacgggccgc cgacacacct
      901 gctcaaggcg ggcacgccca caatgggcgg cacgctgatc ctcatcacca cgctcgtcag
      961 cacgctcctg tgggcggagc tctccaaccg tctggtgtgg acggtgctcg ccgtcacctt
     1021 cgccttcggc ctgatcggct tctacgacga ttacctgaag ctcgtggtgc gcaacccgcg
     1081 gggactcgtg ccgcgctgga agtacttctg gcagtcggtc gccggactcg ccgccgcggc
     1141 gaccctttac tacacggcga ccgaaccggc ggagacgacg ctgttcctgc cgttcttcaa
     1201 gaccttccac accccactga ccgccttcgg cttcatcacg ctcgcctatc tcatgatcgt
     1261 cggcatgagc aacgccgtga acctcaccga cggcctcgac ggactcgcca tcatgccggc
     1321 ggcgctggtc acctcggcgc tcgggatctt cgcctatgcg agcggcaacg cggtgtttgc
     1381 ccactacctg cagattcccg agatccgcgg cgcgggcgag ctgctggtgt tctgcgcctc
     1441 gctcgccggc gccggactcg gcttcctctg gttcaactcg tacccggccc aggtcttcat
     1501 gggggacgtc ggcgcgctcg cgatcggcgc ggccatcggc gtgctggcag tgatcgtcag
     1561 acaggagctc gtcgtgctgt ggatgggcgg cgtcttcgtg ctggagaccg cctcggtgat
     1621 cctgcaggtg gcgtccttca agctgaccgg gcggcgcatc ttccgcatgg cgccgatcca
     1681 ccatcacttc gagctcaagg gctgggcgga gccgaaggtg atcgtgcgct tctggatcat
     1741 ctcgctcctg ctggtgctct cggggctggc gacgctgaag ctgcgatgaa cggcccgacc
     1801 gacaagcgcc cctatgccgt gatcgtggga ctcggccgca ccgggctgtc tgcggcgcgc
     1861 tatctgcgcg cccagggctg gcggctggcg ctgaccgata cgcgcgagca gcccccggag
     1921 ctcgccgcgc ttaccgcgct cgatgcgggc atcccggtgc gcttaggtcg gctcgacgcg
     1981 gcgctactcg agggcgcgct gtgcgtcgtg gcttcccccg gggtgtcgct ggcgcagccg
     2041 ttcttcgccg cggcgcgtgc gcgcggcctc gagatcgtgg gcgatgtcga gctcttcgcc
     2101 cgcgcggtgg ctgcgccggt cgccggcgtc acaggcacca acggcaagag cacggtcacg
     2161 acgctgctcg cgcgcatggc cgagcgctcc gggctcaggg tgcgcgccgg aggtaactta
     2221 ggcgagccgg cactcgacct gctcggctca ggcgccgcgc tctacgtgct cgagctctcg
     2281 agctttcagc tcgagaccgc cacgcacctc gcctgccggg cggcgacggt gctcaacgtc
     2341 accgccgatc acctcgaccg ctacgcctcg ctcgccgagt acgccgccgc caaggcgcgc
     2401 atcttcgtcc gttgtgacac ggcggtcgtg aatctcgacg acccgctggt gatggcgatg
     2461 ccgcgcacgg cgcgccgcac cctgagcttc tcgctgcgcg cggcggtagg tgccgactat
     2521 gaagtggcgc tgcaccacgg cggctggtgg ctgatgcgcc gcgcggcgcc gctcctgccg
     2581 gtggcgcgcc tcaggatcaa ggggctgcac aacgcggcca atgcgctcgc cgcgctcgcg
     2641 ctcggcgagg cgctcgagct gccgctcgag ccgatgctgc gcgagctcga ggaatttccc
     2701 ggcctgccgc accgctcgca gtgggtcgcg gaggtccgcg gcgtcactta catcaacgac
     2761 tccaagggca ccaacgtcgg cgcgacgctg gccgcggtgg gcggcatgac agggccgctc
     2821 gtcatgatcg ctggcggtga cggcaagaac caggacttcg cgccgctcgc ggcggcgttt
     2881 cgcggcaagg tgcgccatgc cgtgctgatc gggcgcgatg cgccgctcct ggcccgcgcg
     2941 ctcgccggcg tgtgcacgct cgagacctgc gcgacgctcc ccgaggcggt gcgcgccgcg
     3001 gcgcgtgcgg cacggcccgg cgatacggtg ctgctgtcgc cagcctgcgc gagcctcgac
     3061 atgttccgcg actacacgca gcgcggcgcc gcgttcgccg cggccgtggg ggagctcgcg
     3121 gcatgagcac ggtaatgcac gagccgctca tgggctttgc ccgctcagcg ggccgcgccg
     3181 ggggcgtgcg cctcgactcg gtcacggtcg cggtgacgct cgccctgact ctcctcggcc
     3241 tcgtcatggt cacttccgcc tccgtctcgg tcgcgagccg cgagacgggc gcgccgttct
     3301 actacctcga gcggcagctg gcgctcgtcg gtgtggggac ggcgtgcgcg gcgctgctct
     3361 tctcgctgcg caccgagtgg ctcgagcgcg cctcggggcc gctgctcgca gtggcgctgg
     3421 cgctgctggt gctggtgctg gtgccggggc tcgggcatgc ggcgaacggc agccgccgct
     3481 ggctgcgtct tgcggccctc aactttcagg tctcggagct cgcgcgcgtg ctggtgctcg
     3541 tgtacgtggc gagctacgcc gtgcggcgcg aggcgcagct gcgcgagggt ctcgccggcc
     3601 tcgccaagcc cctcgcg
//
LOCUS       DATLKL010000581         1658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_51982, whole genome shotgun sequence.
ACCESSION   DATLKL010000581 DATLKL010000000
VERSION     DATLKL010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51982"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VM713_07790"
     CDS             complement(<1..316)
                     /locus_tag="VM713_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84200.1"
                     /translation="MSRSAVLGLVALVGTWGAVQAQETKAGADKAGGKTAESQAEPAG
                     DAGSLLRLCHIDVKGDLKDEWKRLPVAQSSVTEHHFAAAGKSFDYTATAGTLIVRDDE
                     DKP"
     gene            443..1129
                     /gene="trmB"
                     /locus_tag="VM713_07795"
     CDS             443..1129
                     /gene="trmB"
                     /locus_tag="VM713_07795"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HVH84201.1"
                     /translation="MADAAHPRSIRSFVTRAGRITAAQERALTTLWPRYGIEYREEPL
                     ALPALFDRDAPCTLEIGFGNGANLAALARAHPERNYLGVEVHRPGVGRLLLSLEQQRL
                     GNIRVICHDAVEVLERQIAPGSLAEILILFPDPWPKKRHHKRRLIQPQFVALIERALC
                     PDGMLKLATDWQPYALEMLACLSAAPTLRNLAPGGGFAARPAERAPTRFELRGTRLGH
                     EVWDLAFARA"
     gene            complement(1126..>1658)
                     /locus_tag="VM713_07800"
     CDS             complement(1126..>1658)
                     /locus_tag="VM713_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HVH84202.1"
                     /translation="PAYKHRMALKAVTISAAILLLFALGGAAFLQLMGISLEAFRIFG
                     GLLLFLLALEMVFARESGTRTSSDEAAESRRRADISVFPLAFPFIAGPGALATILLWF
                     GRVELPSQAGLFAGFIAAAALVLAIVVALLWVAEPLMRVIGVTGANVASRILGVILGA
                     LAVQFVLDGLRQAFAP"
BASE COUNT          269 a          603 c          527 g          259 t
ORIGIN      
        1 tcggcttgtc ctcgtcgtcg cgcacgatga gggtgcctgc ggtggcggtg tagtcaaagg
       61 acttgcccgc ggcggcgaag tgatgctcgg tgaccgagct ctgcgccacg gggagcctct
      121 tccactcgtc cttcaggtca cccttcacgt cgatgtggca gaggcgcagc agcgagcccg
      181 catcgcccgc gggctccgcc tgcgactcgg cggtcttgcc gcccgccttg tcggcgcccg
      241 ctttggtctc ctgcgcctgc accgcgcccc acgttccgac cagggccacg aggcccaata
      301 ccgcgctcct cgacatcttt atcctcgcct tcttttgcgt gaaaccgcgg gcttatacca
      361 cggccgccgc ctcaccgccc ttaccgccat tacctccctt accgcgcgcc ggcctcgcgc
      421 cacctcggta agatcgctct cgatggccga tgccgcacat ccgcgctcga tccgctcgtt
      481 cgtcacgcgc gccgggcgca tcactgcggc acaggagcgc gcgctcacga cgctgtggcc
      541 gaggtacgga atagagtacc gggaagagcc gctcgcactg cccgcgcttt ttgatcgcga
      601 cgcaccctgc acgctcgaga tcggctttgg caacggcgct aacctcgccg cgctcgcgcg
      661 cgcgcatccc gagcgcaact atctcggcgt cgaggtacac cgtcccggcg tcggcaggtt
      721 gttgctcagc ctcgagcagc agcgcctcgg caacatccgc gtcatctgcc acgacgcggt
      781 cgaggtgctc gagcggcaga tcgcgccggg gagtctcgcg gagatcctca ttctgtttcc
      841 ggacccctgg ccaaagaagc gccatcacaa gcggcggttg atccagccgc agtttgtcgc
      901 actcatcgag cgcgcgttgt gcccggacgg catgttgaag ctcgcgaccg actggcagcc
      961 gtacgcgctc gagatgctcg cgtgcttgag cgccgcgccc accttgcgta atctcgcgcc
     1021 cggcggcggc ttcgcggcgc gtccagccga gcgcgcaccg acccgtttcg agctgcgcgg
     1081 cacgcggctt gggcacgagg tctgggatct cgcctttgcg cgcgcctaag gggcgaacgc
     1141 ctgccgcaac ccgtcgagca cgaactgcac ggcgagcgcc ccgaggatca ccccgaggat
     1201 gcggctcgcg acgttggcgc ccgtgacgcc gatcacccgc atgaggggct ccgccaccca
     1261 cagcagtgcc accacgatcg ccagcacgag cgcggcggca gcgatgaagc ccgcgaacag
     1321 ccccgcctgt gacgggagct cgacgcgtcc gaaccacaag aggatggtgg cgagcgcacc
     1381 ggggccggca atgaacggga atgcgagcgg gaataccgag atatccgcgc gccggcggct
     1441 ctcagccgcc tcgtcgctcg aggtgcgggt gccggattcg cgtgcgaaca ccatctccag
     1501 tgccagcaga aacaacagca agccgccgaa aatccgaaac gcctcgaggc tgatgcccat
     1561 cagttgcaga aacgccgcac caccgagcgc gaacagcagc aggattgcgg cggagatcgt
     1621 cacggccttc agcgccatac ggtgcttgta agcggggc
//
LOCUS       DATLKL010000582         1777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_97418, whole genome shotgun sequence.
ACCESSION   DATLKL010000582 DATLKL010000000
VERSION     DATLKL010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97418"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..383
                     /locus_tag="VM713_07805"
     CDS             225..383
                     /locus_tag="VM713_07805"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HVH84203.1"
                     /translation="MSADDRRAMIAEAAYLRAEGRGFAPGHETEDWLAAEIEVDALLR
                     IGPGGPAQ"
     gene            complement(414..1454)
                     /locus_tag="VM713_07810"
     CDS             complement(414..1454)
                     /locus_tag="VM713_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HVH84204.1"
                     /translation="MRWVAGLTSLSLLALATAPARAAWVNRTEAIMGTRVYVELWADD
                     PERGNAAIEAVMEEMRRIDALMSHYKPDSQLSHINQRAALEPVQVDPELFDLIKLSTH
                     FSEITLGAFDITYASVGYLYNYPLHVHPTEDQIKVALAAVNWRNLQFDAAHHTIRFGH
                     PGMRIDLGGIAKGYAVDRGVAILKARAIEHALVTAGGDSRLLGDHLGRPWLVSIAHPD
                     EPHNPDKVVTRIPLSDAAVSTSGDYERYFDEDGVRYHHIIDPHTGHSANKVRSATIIG
                     PTATQTDGLSKTAFVLGPEKTLEIINRMPQFDAVFVTPTGRMLYSNGLRPPKPRPLGA
                     PPPAANSSPSTR"
     gene            1584..>1777
                     /locus_tag="VM713_07815"
     CDS             1584..>1777
                     /locus_tag="VM713_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84205.1"
                     /translation="MSLARLETLICGALCAAALAAAPLATKAQTTTAAPAASAPVKCK
                     DGTTSPHGGRGACSGNGGID"
BASE COUNT          282 a          566 c          648 g          281 t
ORIGIN      
        1 aggcggcgga cagccgcgga cacctccgct cccctctttg gggtcttgcg cgcccgcggg
       61 cggcagttgc aaagtgactg gcacggaggg ggcctgttgc aaaggaggag gagccgatgg
      121 ccacgcgtcg tacagctgag atacccactg tcgagagccg cgcgccccgc gcacagccaa
      181 aaggaagccg cagcacgccg gcaaaaccgg cgcagcgtgc cgccgtgtcg gcggacgacc
      241 gccgcgcgat gatcgccgag gcggcctacc tgcgcgccga ggggcgcggg ttcgctccag
      301 gtcacgagac cgaggactgg ctggccgccg agatcgaggt ggatgcgctg ctcaggatcg
      361 ggccgggagg cccggcgcag taacggcgcg gcggcgagcg ccgccgcctg cgatcagcgc
      421 gtggacgggg aggaattagc agccggtggc ggggccccca ggggtcgcgg cttgggcggc
      481 cgcaatccgt tggagtagag catccggccc gtcggcgtta cgaacaccgc atcgaattgc
      541 ggcatgcggt tgatgatctc gagggtcttc tcgggaccta agacgaacgc ggtcttcgac
      601 aggccgtcgg tctgtgtggc ggtcgggcca atgatggtgg cgctgcgcac tttgttggcg
      661 gaatggccgg tgtgcgggtc gatgatatgg tggtagcgca cgccgtcctc gtcgaagtaa
      721 cgctcgtaat cacccgaggt cgagacagca gcgtctgaca gggggatgcg ggtcaccacc
      781 ttgtcggggt tatgcggctc gtcgggatgg gcgatcgaca ccagccatgg ccggcccaga
      841 tgatcgccga gcagccggct gtcacctcct gccgtcacca gcgcatgctc gatcgcgcgc
      901 gccttgagga tcgcgactcc acgatcgacg gcatacccct tggcgatccc tccgaggtcg
      961 atgcgcatgc cggggtgccc gaagcggatg gtgtgatggg ccgcgtcgaa ctggaggttg
     1021 cgccagttga ccgccgccag cgccaccttg atctgatcct cggtcggatg gacgtggagc
     1081 ggatagttat agaggtaccc gacgcttgcg taggtgatgt cgaacgcccc gagcgtgatc
     1141 tccgagaaat gcgtcgacag cttgatcagg tcgaacagct ccggatcgac ctgcaccggc
     1201 tcgagggccg cgcgctggtt gatgtgtgac agctggctgt cgggcttgta gtggctcatg
     1261 agcgcatcga tgcggcgcat ctcctccatg accgcctcga tcgccgcgtt gcctctctcg
     1321 gggtcgtcgg cccagagctc cacgtagacg cgggtgccca tgatcgcttc ggtgcggttg
     1381 acccaggccg cgcgtgccgg cgccgtggcg agcgccagca gggagagact cgtaaggcct
     1441 gcaacccatc tcatggggct agcgtaccta gctcgcgcgg cggtgtcccg cgggcgccgc
     1501 ggcgcgtacc ccccggggag gcgattgcgg tattctgcag cctgtctggc cgcggcggtc
     1561 gctcaaaggg ggagtaccga cacatgagcc tcgcgagact tgagaccttg atctgcggcg
     1621 cgctgtgcgc ggcggcgctc gctgcggcgc ccctggcgac caaagcgcag accacgaccg
     1681 cggcgcccgc cgcgagtgcc cccgtgaaat gcaaggacgg cactacctcg ccgcatggcg
     1741 gccgcggtgc ctgcagcggt aacggcggca tcgacaa
//
LOCUS       DATLKL010000583         1667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_6757, whole genome shotgun sequence.
ACCESSION   DATLKL010000583 DATLKL010000000
VERSION     DATLKL010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6757"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VM713_07820"
     CDS             complement(<1..662)
                     /locus_tag="VM713_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVH84206.1"
                     /translation="MTMRHVASAAVGVALLGALPAAYADDGIGHEIKHVLLISVDGLH
                     AVDLSNYVTTHPDSTLAGLSAHGVTYTNASTSSPSDSFPGLASLVTGGSPNSTGFWYD
                     VTWNRAVAPQQTGNVIMGSTGGSCPGTVGAVVELDEGADFDLTRLDGGGGINPAYLPR
                     NATTCQPIYPHAYLRVNTIFEVVHAHGGRTAWTDKHPSYEWTNGPSGQGVDDFYGPEI
                     NS"
     gene            complement(865..>1667)
                     /locus_tag="VM713_07825"
     CDS             complement(865..>1667)
                     /locus_tag="VM713_07825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH84207.1"
                     /translation="GLLQEGYVLGYLLASVVFWLLFVQVGWRGMFIISSASALLVLYI
                     RIGVQESPAWTAGRAPHRASAAELWGAVTHYFPTLCYLVLLMACFNAYSHGSHDLYPA
                     FLQKQHGFPPSVTGPVVIIMNVGALLGGICFGALSERIGRRRAIVLAAALSLPMIPLW
                     AYSQTAVMLAVGGFLMQFMVQGAWGIVPAHLNELSPPSVRAILPAFAYQLGNLAASKM
                     APIQAGIAEAHGNDYAHILSLTMAVVAVALILVTSFGPEARAAELKVA"
BASE COUNT          309 a          535 c          570 g          253 t
ORIGIN      
        1 atcgaattga tctccggacc gtagaagtca tcgacgcctt gaccgctcgg gccattcgtc
       61 cactcgtagg aaggatgctt atccgtccag gcggtacggc caccatgcgc gtgcaccact
      121 tcgaaaatgg tgttgacgcg cagataggcg tgcggataga tcggctgaca cgtcgtggcg
      181 ttgcgcggca gataggcggg gttgatgccg ccgccaccat cgagccgggt gagatcgaag
      241 tcggcgcctt cgtcgagctc gacgaccgct cccaccgtgc cgggacaact gccgccggtc
      301 gagcccatga tgacattccc cgtctgctga ggcgcgacag cgcggttcca cgtcacgtca
      361 taccagaagc cggtactgtt cggggacccg cccgtcacca gtgaggccag cccgggaaac
      421 gaatccgacg ggctcgaggt cgacgcattg gtgtaggtca cgccgtgggc gctcaggccg
      481 gcgagcgtcg agtcggggtg tgtagtcacg tagttcgaca ggtcgacggc gtgcagcccg
      541 tcgacgctga tgagcagtac gtgtttgatc tcgtggccga tgccgtcgtc ggcgtaggcc
      601 gcgggtaagg cgccgagcag cgcgacgccg acggcagcgg acgcaacatg tctcatggtc
      661 atggctgttc tcgcttgagt tggtgttatg gctgcggtgc gaggccaccg cgcgcagccg
      721 ccacgataga gcgaatttgt tacagcctgg cgacgcggga gtcgccgaga ccggacgcca
      781 aggcccagcc ctggcaaggc gcccgcgggt caggactcgc cagccccggc gagcccgccg
      841 ggaattcttg tatcgctcga tcccttaagc gactttgagc tcggcggcgc gtgcctccgg
      901 cccgaacgac gtcaccagga tcagcgccac ggccacaacc gccatcgtca gcgagaggat
      961 gtgcgcgtag tcgttgccgt gcgcttcggc gatgccggcc tggatgggcg ccatctttga
     1021 ggccgcgagg ttgccgagct ggtaggcgaa agccggcagg atcgcgcgca ccgagggagg
     1081 cgagagctcg ttgaggtgcg ccggcacgat gccccacgcc ccctgcacca tgaactgcat
     1141 gaggaagccc ccgaccgcca gcatgacggc ggtctgcgaa tacgcccaca acgggatcat
     1201 gggcagagac agggcggcgg ccagcacgat ggcgcgtctt cggccgatgc gctccgacag
     1261 cgcgccgaag cagatgccgc ccagcaacgc gccgacgttc atgatgatga cgacggggcc
     1321 cgtcacgctc ggggggaatc catgctgctt ctgcaggaag gccggataca gatcatgcga
     1381 cccgtgggag taggcgttga agcacgccat cagcagcacc aggtaacaga gtgtcgggaa
     1441 gtaatgggta accgcgcccc acagctcggc ggccgaggcg cggtgcgggg cccggcccgc
     1501 cgtccatgcg ggggactcct ggactccgat gcggatgtac agcaccagca gcgcggacgc
     1561 cgagctgatg atgaacatgc ctcgccagcc gacctgtacg aacagcagcc agaaaaccac
     1621 cgacgccagc aggtagccga gcacgtaccc ctcctgcagc agccctg
//
LOCUS       DATLKL010000584         2640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_40848, whole genome shotgun sequence.
ACCESSION   DATLKL010000584 DATLKL010000000
VERSION     DATLKL010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40848"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="VM713_07830"
     CDS             <1..1402
                     /locus_tag="VM713_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HVH84208.1"
                     /translation="RLLSEARRHHIRVILDMVLNHTSDQHPWFVEAAGSPQAPHHDFY
                     VWSDGRTGASAQPLPPNNWVSLFGGSAWQYVPALRQFYYHKFYRQQPDLNWRNPAVEQ
                     AMFAAMRFWLDRGVAGFRLDAITELIEDAQLRDEPVLAGTNAQGDPNLSEIYTHNLPE
                     NHAVIRRLRAMIDTYPDERLLIGETYVARTAELDPWYGGAQHDELQLPMDTLVGLGKR
                     LDATLLRRLLSEAQTELHGSQPLLVFDNHDNIRSWDRFGDGVHNAEIARIVAALLLTS
                     RDAALLYQGEEIGQITATPARVEDVRDPIGITGWPKEKGRDGERTPMQWNAEPQAGFS
                     TNPHTWLPVTANYRTLNVQSESADPESLLNWHRRLIALRRSSAALRDGRTVMLDRANP
                     HMLTYARVAPDGAAIVVSLNLSAQPQTVRLGLAAAGARGSRLKTLLASPAPIADTTTA
                     RPVTLAPFAAWVAAVN"
     gene            complement(1422..2168)
                     /locus_tag="VM713_07835"
     CDS             complement(1422..2168)
                     /locus_tag="VM713_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HVH84209.1"
                     /translation="MLPTPFVIPGLARERTVRIYLPPGYEASRRRYPVLYMHDGQNLF
                     DEATAYMGEEWGVDETLNALARRRGLEVIVVGIDNGGAERIHELNAWDNPEFGRGEGE
                     QYLAFLVKVLKPWIDQHYRTQPERRHTAIMGSSMGGLISSFGISRYPEVFGAAGIFSP
                     AYWLAPQVFDYAAAQPPRRDTRVYFYAGGSETDTMIPDTKRMVALLRRAGLPPRNLEV
                     VVNPVGRHNEAAWRAEFPRAVTWLFGAERR"
     gene            2330..>2640
                     /locus_tag="VM713_07840"
     CDS             2330..>2640
                     /locus_tag="VM713_07840"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HVH84210.1"
                     /translation="MKDDLTVSGLLAAPPGARACYVLAHSAGAGMEHPSMRAVATGLA
                     NLKIATLRYQFPYMERHSRRPDPPPLCHATVRAAVAAAASLAPGLPLFAGGRSFGGRM
                     TS"
BASE COUNT          416 a          934 c          870 g          420 t
ORIGIN      
        1 ccgcctgctc tcggaggcac gccggcacca catccgcgtc atcctcgaca tggtgctcaa
       61 ccacacctcg gaccagcacc cgtggttcgt ggaggctgca ggctctcctc aggcgcccca
      121 ccatgacttc tatgtatgga gtgacgggcg cacgggtgcc agcgcgcagc ccctaccgcc
      181 caacaactgg gtgagcctgt tcggcggctc ggcctggcag tacgtaccgg cgctgcgcca
      241 gttctactac cacaaattct atcgccagca gccggatctc aactggcgca atccggcggt
      301 cgaacaggcg atgttcgccg ccatgcgctt ctggctcgat cgcggagtgg cgggctttcg
      361 gctcgatgcc atcaccgagc tcatcgagga tgcgcagctg cgcgatgagc cggtgctcgc
      421 cggcacgaac gcacagggcg acccaaactt gagcgagatc tacactcaca accttcccga
      481 gaaccacgcg gtcatccgcc gtctgcgcgc gatgatcgac acctacccgg acgagcgcct
      541 gctgatcggt gagacctacg tggcccgtac cgccgagctc gatccctggt atggcggagc
      601 gcagcacgat gagctgcagc tgccgatgga cacgctggtg ggcttgggca agcggctcga
      661 tgccaccctc cttcgccgcc tcctcagcga ggcgcagacc gagctgcacg gctcgcagcc
      721 gttgctggtg ttcgacaatc acgacaacat ccgcagctgg gaccgcttcg gcgatggcgt
      781 gcataacgcc gagatcgcgc gcatcgtcgc ggcgctgctg ctcacttcgc gcgacgccgc
      841 gttgctctat cagggtgagg aaatcggcca gatcaccgcg accccggcgc gcgtcgagga
      901 tgtgcgcgac ccgatcggca tcaccggctg gccgaaggag aaggggcgtg acggcgagcg
      961 cacgccgatg cagtggaacg ccgagcctca ggcgggcttc agcaccaacc cgcatacctg
     1021 gctgccggtg acggcgaatt accgcaccct gaacgtgcag agcgagtcgg ccgatcctga
     1081 gtcgctcctc aactggcacc gcaggctgat cgcgctccgc cgcagcagtg cggccttgcg
     1141 cgacgggcgc accgtgatgc tcgatcgcgc caatccacac atgctgacct atgcgcgcgt
     1201 ggccccggac ggtgcggcga tcgtggtctc gctcaacctg tcggcgcagc cgcagacggt
     1261 ccggctcggg ctcgccgcgg cgggcgcgcg cggatcccgg ctgaagaccc tgctcgcgag
     1321 ccccgcaccg atcgccgaca ccaccaccgc gcgcccggtg acgctcgcgc cgttcgcggc
     1381 gtgggttgcc gcagtgaact gagggagcgg cgcggccgcc gtcagcggcg ttcggcaccg
     1441 aacagccacg tgaccgcgcg cggaaattcc gcacgccacg ccgcttcgtt gtgccggccg
     1501 accgggttca ccaccacctc gaggttgcgc ggcggcagac cggcgcgccg gagcagcgcc
     1561 accatgcgct tcgtgtcggg aatcatggtg tcggtctcgc tgccgccggc gtagaaatag
     1621 actcgcgtgt cgcgccgcgg cggctgcgcc gcggcatagt caaacacctg cggggccagc
     1681 cagtaggccg gcgagaagat gccggctgcg ccgaaaacct cggggtagcg gctgatgcca
     1741 aagctcgaaa tgagtccgcc catcgagctg cccatgatcg cggtgtggcg ccgctcaggc
     1801 tgcgtgcgat agtgctgatc gatccagggc ttgaggacct tcacgaggaa cgccaggtac
     1861 tgctcgccct cgcccctgcc gaattccggg ttgtcccagg cgttgagctc gtggatgcgc
     1921 tcggcaccac cgttgtcgat cccgaccacg atcacctcga ggccgcgccg ccgcgcgagg
     1981 gcgttgagcg tctcgtcgac gccccattcc tcgcccatgt aggcggtggc ctcgtcgaac
     2041 aggttctggc catcgtgcat gtacagcacg ggatagcgcc gcctgctcgc ctcataaccg
     2101 ggcggcagat agatgcgtac cgtgcgctcg cgggcgagcc ccgggatcac gaacggcgtc
     2161 ggcagcacgt ggacgttggg ctgtgcggta ctcggtttcg tcgctgccgg aggcgcggtg
     2221 cccgcctcgc atagtgtgag tacattgcat accgcggcga gccacgcgta ttttgtgcgc
     2281 cgtgcccgag cgactgacat cagcggattc tagcccggtc actgtgcagg tgaaggatga
     2341 cctcaccgtg tcggggctac tcgccgcgcc ccctggagcg cgcgcctgtt atgtgttggc
     2401 acacagcgcc ggcgccggca tggagcaccc atcgatgcgt gccgtcgcga cgggtcttgc
     2461 gaacttgaag atcgccacgc tccgctatca attcccctac atggagcgcc actcgcgccg
     2521 cccggatccg ccgcccctgt gccacgccac cgtgcgggcc gcggtggcgg ccgccgcgag
     2581 cctcgcgccg gggctgccgc tcttcgcagg gggacgctcc ttcggcggcc gcatgacatc
//
LOCUS       DATLKL010000585         1671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_97436, whole genome shotgun sequence.
ACCESSION   DATLKL010000585 DATLKL010000000
VERSION     DATLKL010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97436"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VM713_07845"
     CDS             <1..1063
                     /locus_tag="VM713_07845"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HVH84211.1"
                     /translation="AETITRNILTELREPFSINGADVSVRPSIGVSVWPDDGRRVDDL
                     LSHAEVAMALAKERGGEAQFFQRGMTDSMHERLALENDLRRGLAAGEFELSFQPVISA
                     KSGRIITAEGLLRWRHPLRGVIGPSSFIPLAEETGLMIPLGEWVIRKACRYAAAWQLD
                     HGSQIRLAVNPSATQFRHENLVEVIRSALAAEGLDARCLEIELTESAVMTNPEESVGV
                     LKQLRKMGVTIAVDDFGTGYSSLSYLRRFPIDKLKIDRSFVRDLATSRTDESIVRAII
                     SLAHSVGLQVVAEGVETEEQLGCVRALGCDHWQGYYCCEPQPAELFGAMLGDQANPCV
                     GLAAGVARAPRAAGATTLP"
     gene            1173..>1671
                     /locus_tag="VM713_07850"
     CDS             1173..>1671
                     /locus_tag="VM713_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84212.1"
                     /translation="MSLRKCPDCGHDVSSAATACPHCGRPNRRSSSPVELSGILILAV
                     VCAGALIWRLSSRYEAAGEPARAMAPATTIAQPDRDHGGLTVSVGYNRKLFLLRVQNG
                     DSFDWTDCQLSINAQGVSSGYMRDVAVIKRGITEAALLASAEFADAEGRKFDPAAEEV
                     TKLDIG"
BASE COUNT          258 a          610 c          537 g          266 t
ORIGIN      
        1 cgccgagacc atcacccgca acatcctgac cgagctgcgc gagcccttct ccatcaacgg
       61 cgccgatgtc agcgtccgcc cgagcatcgg cgtcagcgtg tggccggacg acggccgccg
      121 cgtggatgac ctgctgtctc acgccgaagt cgccatggcg ctcgccaagg agcgcggtgg
      181 cgaagcgcag ttcttccagc gcggcatgac cgactcgatg cacgagcggc tggcgctcga
      241 gaatgacctg cgccgcggcc tcgcggccgg cgagttcgag ctgtccttcc agccggtgat
      301 ctccgccaag agcggccgca tcatcaccgc cgaggggctg ctgcgctggc gccatccgct
      361 gcgcggcgtg atcggcccct cctctttcat tccgctcgcc gaagagaccg ggctcatgat
      421 cccgctcggg gaatgggtga tccgcaaggc ctgtcgctac gccgccgcct ggcaactcga
      481 ccacggctcg cagatccgcc tcgcggtcaa cccgtccgcc acccagttcc gccatgagaa
      541 cctggtcgag gtcatccgct cggcgctcgc cgcggaaggc ctcgatgccc gctgcctcga
      601 gatcgagctc accgagagcg cggtcatgac caaccccgag gagtcggtcg gagtgctcaa
      661 gcagctgcgc aagatgggcg tcaccatcgc ggtcgatgac ttcggtaccg gctactcgag
      721 cttgagttat ctgcgccgct tcccgatcga caagctgaag atcgaccgca gcttcgtacg
      781 cgacctcgcc acgagtcgca ccgatgaatc gatcgtgcgg gccatcatct cactcgccca
      841 cagcgttggc ttgcaggtgg tggccgaggg agtcgagacc gaggagcagc tcggctgcgt
      901 gcgcgccctc ggctgcgatc actggcaggg gtattactgc tgtgagcccc agcccgcaga
      961 gctcttcggc gcgatgctgg gcgatcaggc caacccctgc gtcgggctcg ccgccggtgt
     1021 cgcccgggcg ccgcgtgcgg ccggcgcaac gactctcccc tagcctctct gccgagccgc
     1081 gctcaggaag cggcagcgcc gcgccgccga gactttgccc gcactgatcc gaagcgacac
     1141 aatacggcat cgcgcgcttg ggggtcagcc gggtgagttt gcgcaagtgc cctgactgcg
     1201 gccacgacgt ctcgtcggct gcgaccgcct gccctcactg cggccggccc aaccgccgca
     1261 gctcctcccc cgtcgagctc tcgggaattc tcatcctcgc cgtcgtctgc gccggcgctc
     1321 tgatctggcg cctcagctcg cggtacgagg ccgccggcga gcccgcccgc gcgatggctc
     1381 cggcgaccac gattgcgcag cccgaccgcg accacggtgg gctcaccgtc agcgtcggct
     1441 acaaccgcaa gctctttctc ctgcgcgtgc agaacggcga ctcgttcgac tggaccgact
     1501 gtcagctgag tatcaacgcg cagggagtct cctcgggcta catgcgcgac gttgcggtca
     1561 tcaagcgcgg aatcaccgaa gcggcgttgc tcgcgagcgc cgagttcgcc gacgccgagg
     1621 gccgcaagtt cgacccggcc gccgaggagg tgacgaagct cgatatcgga t
//
LOCUS       DATLKL010000586         2733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_63455, whole genome shotgun sequence.
ACCESSION   DATLKL010000586 DATLKL010000000
VERSION     DATLKL010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63455"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VM713_07855"
     CDS             <1..692
                     /locus_tag="VM713_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HVH84213.1"
                     /translation="ALAGALLVVEPDLGAATVLFATGFGLLFLAGARLRYVLATMALA
                     AGGFALLALGSSYRVRRLTAFLDPWADPYHSGFQLTQSLIAIGRGQWLGVGLGESVQK
                     LFYLPEAHTDFLFAVLAVELGLAGVVLTLGLFLILIWRAFHIARLASEAGLKFPAFLA
                     AGFGLWVGIQTFINIGVNMGVLPTKGLTLPLMSYGRSSLVVTLAWVGLLLRVYHEAMR
                     EGRGAATVYSP"
     gene            689..1774
                     /gene="murG"
                     /locus_tag="VM713_07860"
     CDS             689..1774
                     /gene="murG"
                     /locus_tag="VM713_07860"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HVH84214.1"
                     /translation="MSRPILIMAGGTGGHVFPALALARLLRERSLEVVWLGTERGLEA
                     RLIPAEGIPLERLSVAGLRGKGVFAWLTAPFRLTLALAQALAIMRRHRPLVVVGLGGF
                     VTGPGGVAAWLTRRPLVIHEQNAIAGFTNRCLAHLARVVLEAFPDSFGRDVHARLIGN
                     PVRRDISDVPPPRERFAGRAGPIRLLVFGGSQGAARLNTVVPFALARLAGRLTFDVRH
                     QAGERWLEEGRAAYARAGVRAEVRPFIADMAEAYAWADLVVCRAGALTVSELAAVGIG
                     AVLVPFPGAVDDHQAANAQYLVRESAAVVIADRELTAERLAAELERLCTGRGKLLAMA
                     ERARQLATPRAADELAASCLALIPRAA"
     gene            1744..>2733
                     /locus_tag="VM713_07865"
     CDS             1744..>2733
                     /locus_tag="VM713_07865"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HVH84215.1"
                     /translation="MPGAHPESGMMKERRTLPERPAIERRVHPDRRDRMRRINTIHFV
                     GIGGSGMSGIAEVLLSLGYAVQGSDARSSAVTERLSSLGARIAIGHAAANIGAADVVV
                     ASSAVTRGNPEVEAALERRTPVVSRAEMLGELMRFRYSIAVAGTHGKTTTTSLLASIL
                     SEGGEDPTFVIGGRLKSAASGAQLGAGRYLVAEADESDASFTHLQPLIAIVTNIDNDH
                     LITHGGDFGLLKQSFLDFLHNLPFYGLAVLGVDDAEVRSILSEVGRPILSDGLDAGAD
                     VRALHVAPAGLRTRFEVVRAGGAPLAVTLNLPGAHNVRNSLAAIAAATELVIDD"
BASE COUNT          331 a         1012 c          955 g          435 t
ORIGIN      
        1 tggccctagc cggggcactg ctcgtagtcg agccggacct cggcgctgcg accgtgctgt
       61 tcgcgaccgg ctttggcctc ctgttcctcg ccggcgcccg gctgcgctac gtgctcgcga
      121 cgatggcgct cgccgcgggc ggcttcgcgc tgctcgcgct cggctcgagc taccgggtgc
      181 gccgcctcac ggcctttctc gatccatggg ccgaccctta ccacagcggc ttccagctga
      241 cacagtcgct gatcgccatc gggcgcggcc agtggctcgg tgtcgggctc ggcgagagcg
      301 tacagaagct gttttatcta cccgaggcgc acaccgactt cctgttcgcg gtgctcgccg
      361 tggagctcgg gctcgccggc gtcgtgctga cgctcgggct gtttctcatc ctcatctggc
      421 gcgccttcca catcgcgcgg ctcgcctcgg aggcggggct caagttcccc gcgttcctcg
      481 cggccggctt cggcctctgg gtcggcatcc agaccttcat caacatcggc gtcaacatgg
      541 gcgtgctgcc gaccaagggg ctgacgctgc cgctcatgag ctacggccgc tcgagcctgg
      601 tggtgacact cgcgtgggtg ggcctgctgc tgcgcgtcta ccacgaggcg atgcgcgaag
      661 gccgcggcgc ggccacggtg tactcgccat gagccgcccg atcctcatca tggcgggcgg
      721 taccggcggt cacgtgttcc cggctctggc gctggcgcgg ctgctgcgcg agcggagcct
      781 ggaggtcgtg tggctcggga ccgagcgcgg gctcgaagcg cgcctcattc ccgccgaggg
      841 cattccgctc gagcggctgt cggtcgcggg tctgcgcggc aagggcgtgt tcgcctggct
      901 aaccgctccg ttccgcctga cactcgcgct cgcgcaggcg ctcgcgatca tgcggcgcca
      961 ccggccgctg gtggtcgtgg gcctcggtgg tttcgtcacc ggtcccggcg gggtcgccgc
     1021 ctggctcacg cgccggccgc tggtcataca cgagcagaac gcgatcgccg ggttcaccaa
     1081 ccgctgcctg gcgcacctcg cgcgcgtggt gctcgaggct tttccggaca gcttcggccg
     1141 cgacgtgcac gcccggctca tcggcaaccc cgtgcggcgc gacatcagtg atgtcccgcc
     1201 gccccgggag agattcgcgg gccgcgccgg accgatacgc ctcctggtgt tcggcggcag
     1261 ccagggcgcc gcgcgcctga acacggtcgt gccttttgcg ctcgcgcgcc tcgcggggcg
     1321 cctcaccttc gacgtgcgcc accaggcggg cgagcgctgg ctcgaggagg gccgcgctgc
     1381 ttacgcgcgc gccggggtgc gcgcggaggt gcgccccttt atcgcggaca tggccgaggc
     1441 ctacgcctgg gcggatttgg tggtctgccg cgcgggggca ctcaccgtgt cggagctcgc
     1501 tgccgtcggc atcggcgcgg tgctggtgcc gtttccggga gccgtggacg atcaccaggc
     1561 cgccaacgcg cagtacctgg tgcgcgagag cgccgcggtg gtcattgccg accgggagct
     1621 caccgccgag cggctcgccg ccgagctcga gcggctgtgc acggggcgcg gcaagctcct
     1681 cgccatggcc gagcgcgccc ggcagctcgc cactccgcgc gcggcggatg agctcgccgc
     1741 gtcgtgcctg gcgctcatcc cgagagcggc atgatgaagg agcggcgcac cctcccggag
     1801 cgtcccgcga tcgagcggcg cgtgcatccc gaccggcgcg accgcatgcg ccgcatcaac
     1861 accatccact tcgtcggcat cggcggcagc ggcatgagcg gcatcgccga ggtgctgctc
     1921 tccctcggct acgcggtgca ggggtcggat gcgcgcagca gcgccgtgac cgagcgccta
     1981 agctcgctcg gcgcgcgcat cgcgatcggc cacgccgccg ccaacatcgg cgcggcggat
     2041 gtcgtcgtcg cctcgagcgc cgtgacgcgc ggtaacccgg aagtcgaggc ggcgctcgag
     2101 cgccgcactc cggtggtgtc gcgcgccgag atgctcggcg agctgatgcg cttccgctac
     2161 tcgatcgcgg tcgccggcac ccacggcaag accaccacca ccagcctcct cgccagcatc
     2221 ctctccgagg gcggcgagga tccgaccttc gtcatcggcg ggcgtctgaa gagcgccgcc
     2281 agcggcgcgc agctcggcgc gggacgctac ctggtggcgg aggccgacga gagcgacgcc
     2341 tccttcactc acctgcagcc gctcatcgcg atcgtcacca acatcgacaa cgatcacctc
     2401 atcacccacg gcggcgactt cgggctgctc aagcagagct tcctcgactt cctccataac
     2461 ctgcccttct acgggctggc cgtgctcggc gtagacgacg ccgaggtgcg cagcatcctg
     2521 agcgaggtcg ggcggccgat cctctccgac ggcctcgatg cgggcgccga cgtgcgcgcg
     2581 ctgcacgtgg ctcccgcggg cctcaggact cgcttcgagg tggtacgcgc cggcggcgct
     2641 ccgctcgccg tcacgctcaa cctccccggc gcgcacaacg tgcgcaactc gctcgcggcg
     2701 atcgctgcgg ctaccgagct cgtcatcgac gat
//
LOCUS       DATLKL010000587         1964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_52030, whole genome shotgun sequence.
ACCESSION   DATLKL010000587 DATLKL010000000
VERSION     DATLKL010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52030"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1672
                     /locus_tag="VM713_07870"
     CDS             <1..1672
                     /locus_tag="VM713_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVH84216.1"
                     /translation="RAAIATMGDKIASKRLAQKAGVDTVPGHNDVVPDAGAAAKIARE
                     IGYPVMIKAAAGGGGKGMRIARSESELRDGLRLARNEAKAAFGDDRVFLEKFIEEPRH
                     IEIQVLADAHGNVIHLGERECSIQRRHQKVIEEAPSPLLDEKTRNALGARAVALAKAV
                     DYLSAGTVEFIVGRERKFYFLEMNTRLQVEHPVTELVTGIDIVEQMIRVAAGERLALR
                     QADIKMQGWAIEARIYAEDPSRKFLPSVGRLVRYLPPPADGQRVRVDTGVYEGGGISI
                     YYDPMIAKLIAGGRDRGEALARLRAALDAFYIRGVNHNIPFLAAATAHRRLVEGRLST
                     EFIAEVFGESFTPPLLGKEEARRLVAVAAFLNRRQVERECRISGQLIGHEAKPGADYV
                     VMLGDEEHAVTLAPADGGYDVTIEGQPSGVRGAWRIGEPLFKGSVNGEALTVQVDRHG
                     IAYRLALGGREVEVRVVSPQAAASLRLMPKKAQPDMSRYLISPMPGLLVSLAVKVGQE
                     VKAGEELAVVEAMKMENILRAERDGVVAKLHVKPGDSLAVDQAILEFA"
     gene            1681..>1964
                     /locus_tag="VM713_07875"
     CDS             1681..>1964
                     /locus_tag="VM713_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HVH84217.1"
                     /translation="MAAGGATKCVRVRIEGKVQGVWFRAWTAEEATTRVLRGWVRNRR
                     DGSVEAQFAGPAERVDDMTAACRRGPPLARVDGIIAAPVDDDGEAGFRALP"
BASE COUNT          327 a          638 c          692 g          307 t
ORIGIN      
        1 gcgcgcggca attgcgacga tgggcgacaa gatcgcgtcg aagcgccttg cccagaaggc
       61 tggcgtcgat actgtgccag gccacaacga tgtcgtcccg gacgccggcg cggcggccaa
      121 gatcgcgcgg gaaatcggct atccggtcat gatcaaggcc gcggccggcg gcggcggaaa
      181 gggcatgcga atcgcccgca gcgaatcaga gctgcgcgat ggtttgcggc tcgcccgcaa
      241 cgaggcgaaa gcggccttcg gcgacgaccg cgtcttcctc gagaagttca tcgaggagcc
      301 tcggcatatc gagatccagg tgctcgccga cgcgcacggc aacgtgatcc atctcggcga
      361 gcgcgagtgc tcgatccagc gccggcacca aaaggtgatc gaggaggcgc cgagcccact
      421 cctcgatgag aagacgcgca atgcgttggg cgcgcgggcg gtggcgctgg ccaaggcggt
      481 cgactacctt tccgccggaa cggtcgagtt catcgtcggc cgagagcgaa agttctactt
      541 cctcgagatg aacacgcgcc ttcaggtcga gcatccggtg accgagctcg tcaccggcat
      601 cgatatcgtg gagcagatga tccgcgtcgc tgcgggcgaa cggctcgccc tgcgccaagc
      661 ggatatcaag atgcagggct gggcgatcga ggcgcgcatc tatgcagagg acccgtcacg
      721 caaattcctg ccctcggtgg gccgcctcgt acgctacctg ccgccgccgg ccgatggtca
      781 gcgcgtgcgc gtagacaccg gcgtatacga gggcgggggg atctcgatct actacgatcc
      841 catgatcgcg aagctgatcg cgggggggcg cgatcgcggc gaggcgctgg cgcgcctgcg
      901 cgcggctctc gacgctttct acatccgcgg ggtcaatcac aatattccgt tcctcgccgc
      961 ggccaccgcc catcggcgcc tcgtggaggg tcgcctctcg acagaattca tcgccgaggt
     1021 gttcggcgag tccttcactc cacctctcct cggaaaggaa gaggcgcgcc gactcgttgc
     1081 cgtcgccgcc ttcctcaacc gccgacaggt cgagcgcgag tgccgcatca gcggccagct
     1141 catcggccat gaggcgaagc ccggcgcgga ttatgtggtc atgctcggcg acgaggagca
     1201 tgccgtgacg ctcgcacctg ccgatggcgg ctacgatgtc acgatcgagg gccagccgag
     1261 cggcgtgcgc ggcgcgtggc gtattggcga gccgcttttc aagggcagcg tcaacggcga
     1321 agcgctgacc gtgcaggtcg atcggcacgg gatcgcttat cgtctcgccc ttggcgggcg
     1381 tgaggtcgag gtccgcgtcg tctcgcctca ggccgccgcg tccctcaggc tcatgcccaa
     1441 gaaggcgcag cccgatatgt cgcggtacct catatcgccc atgcccgggc ttctggtctc
     1501 gctcgccgtc aaggtcgggc aagaggtgaa ggctggcgag gagctcgctg tggtcgaggc
     1561 gatgaagatg gagaatatcc tgcgcgccga gcgcgacggg gtggtagcca agctccacgt
     1621 taagccgggc gatagcttgg ccgtcgacca ggcgatcctc gagtttgcct gacgaaagcc
     1681 atggccgcgg gcggcgcgac caaatgcgtg cgggtgcgca tcgagggcaa ggtgcagggc
     1741 gtttggttcc gcgcctggac ggccgaggag gcgaccacgc gcgtcctgcg cggctgggtg
     1801 cgcaaccgcc gcgacggcag cgtcgaggcc caattcgccg gaccggccga gcgggtcgac
     1861 gatatgaccg cggcatgccg tcggggtccg ccgctcgccc gggtggacgg catcatcgcc
     1921 gctcccgtcg acgatgacgg cgaggccggc ttccgcgcgc tgcc
//
LOCUS       DATLKL010000588         1706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_176082, whole genome shotgun sequence.
ACCESSION   DATLKL010000588 DATLKL010000000
VERSION     DATLKL010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176082"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..1271)
                     /gene="secF"
                     /locus_tag="VM713_07880"
     CDS             complement(336..1271)
                     /gene="secF"
                     /locus_tag="VM713_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HVH84218.1"
                     /translation="MEFFSHQASYPFMATRKVWYTLSAVLMIVSLGSFFLRGVNLAID
                     FTGGVSAEASFPQAANVDQVRARLTAAGFRDPSVQNFGSSRDIAIRLPPDPKQTSGAI
                     RTQLETALRAIDPGAQVVQLEVVGPQVGTELKVSAAWALFFTLLGIFLYIAFRFHTWR
                     LSLGAILAVLHDPILVLGFFAVTQMSFDLAVVAAILAVIGYSLNDTVVVFDRIRERFE
                     GNRRSPPDVVLDQSINQTLSRTIMTKVVTSIVVVALLFLGGPVLKGFSAALLIGIIAG
                     TYSSIYISSAIALDCGLTAEHVFPTVKKAALDHLP"
     gene            complement(1307..>1706)
                     /locus_tag="VM713_07885"
     CDS             complement(1307..>1706)
                     /locus_tag="VM713_07885"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVH84219.1"
                     /translation="ANVVLLTALLSMMRASLSLPGIAGIILTVGMAVDANVLIYERIR
                     EEIRKGVTPQAAIRAGFEKAFSAIADSNITTLIAGVVLWVLGTGAIRGFAVVLTLGIA
                     TSMFTSLMGSRALLTLMYGGRRKLARLAIG"
BASE COUNT          331 a          543 c          599 g          233 t
ORIGIN      
        1 agcgagtggt gaatctcgag cacgctcttg ccgagcagga agaggccccc ggcgagcagc
       61 accaggtcgc gtgccgagaa cacctgcccg gcgaagtgca gccatggctg cgtgagatgg
      121 gtcagccata cgatcgagaa gagcagcgcg atgcgggtgg ccatcgccag gaccagaccg
      181 ccgatgcgcg cggttcgctg ctggtgcggc ggcaggcgcc ccaccaggat cgcaaggaag
      241 atcagattat cgacgccgag tacgatctcg agcgccgcca gcgtgacgaa cgccagccag
      301 gcgttgggat cggcgatcag cgtggccgca cctcgttacg gcaggtgatc gagagccgcc
      361 ttcttgaccg tcgggaacac gtgctcggcg gtgaggccgc agtcgagcgc gatggcgctc
      421 gagatgtaga tcgaggagta ggtgccggcg atgatgccga tgagcagtgc cgccgagaac
      481 cccttgagca ccggtccgcc gaggaacaac agcgccacca ccacgatcga ggtgaccacc
      541 ttggtcatga tggtgcgcga cagcgtctgg ttgatcgact ggtcgagcac cacgtcgggc
      601 ggcgagcggc gattgccctc gaagcgctcg cggatgcggt cgaacaccac gacggtatcg
      661 ttgagcgagt agccgatgac ggcgaggatc gccgccacta ccgcgaggtc gaaggacatc
      721 tgcgtgaccg caaagaaccc gagcaccagg atcgggtcgt gcagcaccgc gaggatcgcg
      781 ccgagcgaga gcctccaggt gtgaaagcgg aaggcgatgt agaggaatat cccgagcagc
      841 gtaaagaaca gcgcccacgc cgcgctcacc ttcagctcag tccccacctg cggcccgacc
      901 acctcgagct gcaccacctg cgcgccgggg tcgatcgcgc gcagggcggt ctcgagctgc
      961 gtgcggatcg ccccggaggt ctgcttcgga tccggcggca ggcggatggc gatgtcgcgc
     1021 gaggagccga agttctgcac cgaaggatcg cggaagcccg ccgcggtgag gcgcgcgcgc
     1081 acctggtcca cgttggccgc ctgcgggaag ctcgcctcgg cgctcacgcc gccggtgaag
     1141 tcgatggcga ggttcacgcc ccgcaggaag aacgagccga gcgagacaat catgagcacc
     1201 gccgacagcg tgtaccacac cttgcgcgtg gccatgaacg ggtagctggc ctgatggctg
     1261 aagaattcca cgtttgatcc ttgcaggctt acccggacag cggcgctcag ccgatcgcga
     1321 ggcgggcgag cttacgccgg ccgccgtaca tgagcgtgag gagcgcgcgg ctccccatga
     1381 gggaggtgaa catcgaagtg gcgatgccga gggtgagcac caccgcgaaa ccgcggatcg
     1441 cgccagtacc gagcacccac agcacgacgc ccgcgatcag cgtcgtgatg ttggagtcgg
     1501 cgatcgccga gaaggccttc tcgaagccgg cccggatcgc cgcctgcggc gtgacgccct
     1561 tgcggatctc ctcgcggatg cgttcgtaga tcagcacgtt cgcatccacc gccatgccga
     1621 cggtgaggat gatgccggcg atgccgggga gcgagagcga ggcccgcatc atggacagca
     1681 acgcggtcag cagcaccacg ttcgcg
//
LOCUS       DATLKL010000589         1917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_97456, whole genome shotgun sequence.
ACCESSION   DATLKL010000589 DATLKL010000000
VERSION     DATLKL010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97456"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1769)
                     /gene="ilvD"
                     /locus_tag="VM713_07890"
     CDS             complement(30..1769)
                     /gene="ilvD"
                     /locus_tag="VM713_07890"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HVH84220.1"
                     /translation="MASPKTSADKFDKSKLPSRHVTVGDTSAPHRAFYYAMGMTEADI
                     ARPLVGVVTTWNEAAPCNIALSRQAQAAKLGVKAGGGCPREFTTITVTDGIAMGHAGM
                     KSSLVSREVIADSVELTMRGHCYDALVGIAGCDKSLPGIMMAMVRLDVPAVFLYGGPI
                     LPGKFKGRDVTIVDVFEGVGARAVGRMTAAELHALECAACPSAGSCGGQFTANTMACV
                     SEAMGLALPGSAMPPAAYESRDAFAEAAGRAVMELLRKRIRPRHIVTRKALENAATVV
                     AASGGSTNAALHLPAIAHEAGIAFTFDDVAKIFRRTPYIADLKPAGKYVAKDLYEVGG
                     VPVLMKALFDGGHLHGDSLTVTGKTIAENLKDVEMPKNQDVIYSTERPISSTGGVVGL
                     KGNLATEGAIVKIAGMKRLQFSGNARCFDCEEDALEAVLARKIKPGEVVVVRYEGPRG
                     GPGMREMLSTTSAIYGQGLGEQVALITDGRFSGGTRGFCIGHIGPEAAVGGPIALLKD
                     GDVIKIDAAKGTLDVDLAEAELARRRKAWKPRRHDFQSGTLWKYAQTVGPACYGAVTH
                     PGAKAETHCYADI"
BASE COUNT          300 a          677 c          614 g          326 t
ORIGIN      
        1 gtgaacgccg cgctcgcccc gatctaggct cagatgtccg cgtagcaatg cgtctccgcc
       61 ttggcgcccg gatgggtgac agcgccgtag caggccggtc cgaccgtctg ggcgtatttc
      121 cacagcgtgc ccgactggaa atcgtgccgg cgcggcttcc aggccttgcg ccgccgcgcc
      181 agctccgcct cggcgaggtc cacatcgagc gtccctttcg cggcgtcgat cttgatgacg
      241 tcgccgtcct tgaggagcgc gatgggcccg cccaccgccg cctccggccc gatatggccg
      301 atacagaagc cgcgcgtgcc gccggagaag cggccgtcgg tgatgagcgc cacctgctcg
      361 cccagaccct ggccgtagat ggcgctcgtg gtcgacagca tctcgcgcat gcccggaccg
      421 cccctcgggc cttcgtagcg cacgaccacc acctcgcccg gcttgatctt gcgcgcgagc
      481 accgcctcga gggcgtcctc ctcgcagtcg aaacagcggg cgttgccgga aaactgtagg
      541 cgcttcatgc ccgcgatctt cacgatcgcg ccctcggtcg cgagattgcc cttgaggcct
      601 acgaccccgc cggtcgagct gatcggccgc tccgtcgaat agatcacgtc ctggttcttg
      661 ggcatctcca cgtccttgag attctcggcg attgtcttgc cagtcaccgt cagggaatcc
      721 ccgtggagat ggccgccgtc gaacagcgcc ttcatcagca ctggcacgcc gcccacctcg
      781 tataggtcct tggcgacgta cttcccggcc ggcttcaaat cggcaatgta gggagtgcgc
      841 cggaagattt ttgcgacatc gtcgaacgtg aatgcgatgc ccgcctcgtg cgcgatcgcc
      901 ggcaaatgca acgcggcatt cgtcgagccg cccgacgccg cgaccacggt cgcggcattc
      961 tcgagcgcct tgcgcgtgac gatgtgacgc ggacgaatgc gtttgcgcag cagctccatg
     1021 accgcgcgac ccgcagcctc ggcgaaggca tcgcggcttt cgtaggcggc aggcggcatg
     1081 gccgagccgg gcagcgctaa tcccatcgcc tcggagacgc aggccatcgt attggccgtg
     1141 aactggccgc cacaggaccc ggccgaggga caggcggcgc actcgagcgc gtgcagctcg
     1201 gcggccgtca tccggccgac ggcgcgggca cccacgccct cgaatacgtc gacaattgtg
     1261 acgtcgcggc ccttgaattt gccgggcagg atcgggccgc cgtacaggaa gaccgcgggc
     1321 acgtcgagcc gcaccatggc catcatgatg cccggcagcg acttgtcgca gccggcgatc
     1381 ccgacgagcg cgtcatagca atgtccgcgc atggttagct cgaccgaatc cgcgatcacc
     1441 tcgcggctga cgagtgagga cttcatgcct gcgtgaccca tggcgatgcc gtcggtcacc
     1501 gtgatggtgg tgaactcgcg cgggcagccg ccaccggcct tcacccccag ctttgcggcc
     1561 tgggcctgac gggagagcgc gatattgcac ggcgccgcct cgttccaggt tgtgaccacg
     1621 cccacgaggg gccgcgcgat gtcagcctcg gtcatcccca tcgcgtagta gaaggcgcga
     1681 tggggcgcgc tcgtgtcacc caccgtgacg tgacggctcg gcagctttga cttgtcgaat
     1741 ttgtcagcgg atgtcttcgg cgaggccata ggctcccccc gtcctttctg caatcggcgc
     1801 cagaataatg gccgctaagc gcttcgacca gcgcgaaaaa tgccggccgg ccatgccgcc
     1861 ggccgagacc ggactcaggg cttcgcctcg gcctcgttcg cgcctttgac ggccgcc
//
LOCUS       DATLKL010000590         1550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_52090, whole genome shotgun sequence.
ACCESSION   DATLKL010000590 DATLKL010000000
VERSION     DATLKL010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52090"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="VM713_07895"
     CDS             <1..1397
                     /locus_tag="VM713_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84221.1"
                     /translation="GSWIGALLLSCLCLALPTHAAAADDTAAAAKSDESDRLAALERQ
                     LKESLELIRQLSARVKELEAAQAARPQAAQVPAPPASAGPAATAAAASGPPAAPPDTA
                     KRLAQVEQTVSEIAAGAAQRAEDTGMPMHGFADVGVGNHNAEFHEYQGADIAELDFFL
                     TPRLGARTRALFELNFEVGESGQVGVDLERAQIGYQIGDSATLWLGRFHTPYGFYNTA
                     FHHGQWIAIALRRPRFIEFEDHGGIMPAHTVGLWLTGSERVGDMKVTYDAYLGNSQRI
                     FNGILDMNNAGVPRGSAIFGGNLGLLPGGVLDGLKVGVDAFQTRIEDQDATPAAFPQV
                     TPYFTQVKSYGAYAVYDTDRWEDIAELHFFDDTDLTGHTGSHHSDAGFIQMGYRAGRY
                     TPYGRYERGSFQQSDPYFAAQVNGNSYDREALGLRFDIDLSSALKIEFAKTSFSDRII
                     RTWGEALVQYAIRF"
BASE COUNT          263 a          541 c          492 g          254 t
ORIGIN      
        1 gaggctcctg gatcggcgcg ctgctgctgt cctgcctttg cctcgcgctc cccacgcacg
       61 cagcggcggc cgacgataca gcggctgcgg cgaagagcga tgagagcgat cgccttgccg
      121 cgctcgaacg ccagctcaag gagagcctcg agctgatccg ccagctctcg gcccgggtga
      181 aggagctcga ggcggctcag gcggcccggc ctcaggcggc tcaggtcccc gcaccgcccg
      241 ccagtgccgg cccggcggcc acggcggctg cagcgagtgg ccccccggcg gcgcccccgg
      301 acaccgccaa gcgtctcgcg caggtagagc agacggtttc ggagatcgct gcaggtgccg
      361 cccagcgtgc cgaggacacc ggcatgccga tgcacggctt tgccgatgtc ggagtcggca
      421 accacaacgc agagttccac gagtaccagg gcgctgatat cgcggagctg gatttcttcc
      481 tcaccccgcg gctcggggcg cgcacccgcg cgctctttga gctgaatttt gaggtggggg
      541 agtcaggtca ggtgggagtc gatctggaac gggcgcagat cggctaccag atcggggact
      601 ccgctacctt gtggctgggg cgcttccata ccccctacgg cttctacaac accgccttcc
      661 accacgggca gtggatcgca atcgcgctgc gccgcccccg cttcatcgaa ttcgaggatc
      721 acggtggcat catgcccgcg cacacggtgg gcctgtggct cacgggatcg gagcgcgttg
      781 gcgacatgaa agttacctac gacgcctatc tcggcaacag ccagcggatc ttcaacggca
      841 tcctcgacat gaataacgcg ggcgtgcccc gcggcagtgc gattttcggc ggcaatctgg
      901 gtctgctgcc cggcggggtg ctcgacggac tgaaggtcgg agtcgacgcg ttccagacgc
      961 gcatcgagga ccaggatgca acgcccgcgg cgttcccgca ggtgaccccc tacttcaccc
     1021 aggtgaagag ttacggtgcc tacgccgtct acgacaccga ccgctgggaa gacatcgcgg
     1081 agctgcattt cttcgacgac acggacctca cggggcatac cggctcgcac cacagcgacg
     1141 ccgggttcat ccagatggga taccgtgccg gccgctatac cccgtacggc cgctacgagc
     1201 gtggatcgtt ccagcagtcg gacccctact tcgccgcaca ggtcaacggc aactcctacg
     1261 atcgcgaggc gctcggcctg cgcttcgaca tcgatctttc ctccgccctc aagatcgagt
     1321 ttgccaagac cagcttcagc gaccggatca tcaggacctg gggcgaagcg ctcgtccagt
     1381 acgccatccg cttctgaatg atgcgaatcc cggccaagct tctcctggca actgtgctcg
     1441 ccgcggcggg cagcgccgcc cgtgccgacg acctctacat cgtctgcaac ctgcgcccgg
     1501 cgattaccgc cgccgacgtg cgcgacctgt tcgtcggcga gaagcagttc
//
LOCUS       DATLKL010000591         1623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_221190, whole genome shotgun sequence.
ACCESSION   DATLKL010000591 DATLKL010000000
VERSION     DATLKL010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221190"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VM713_07900"
     CDS             complement(<1..706)
                     /locus_tag="VM713_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVH84222.1"
                     /translation="MSLTTLLALAALLVAGGGAADYLLYPPTHAPLSWLPLVLAGAAL
                     LALLGALFVALRTRRTVERQRASADARLQENDRNQQAILRLLDELSSLADGDLTVQAT
                     VTEDITGAIADSINYAVDALRGLVTTINASAIQLDSATRQTQALSSHLAKASGAQSKQ
                     IGSATESAASMAGSVEEVSGNAERASDVARHSVEIAHKGGDAVRRTIDGMNTIRETIQ
                     ETSKRIKRLGESSQEIG"
     gene            complement(703..1281)
                     /locus_tag="VM713_07905"
     CDS             complement(703..1281)
                     /locus_tag="VM713_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HVH84223.1"
                     /translation="MPEPVTNSEIDLKSLRDRPFELLAELERRGRAVSAQVSEQGASG
                     REWVGVALRMAGDLYLVAREETREVLGVPASTTRVPGAKAWIRGLANVRGQLLPIIDL
                     RQFLGSGVTPVTRNTRVLVVNHREIPAGLLVDEVLGFRRFAESEFSGEAPPTVARCER
                     YLAGAFRRAHEQWPVLSLRSVLESPAFAEAAA"
     gene            complement(1318..>1623)
                     /locus_tag="VM713_07910"
     CDS             complement(1318..>1623)
                     /locus_tag="VM713_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH84224.1"
                     /translation="LEKHGFRTAAAADGAEGVRLAREMSPDLIFMDIVMPGMNGYQAT
                     RALANDPRTRTIPIIMVTSKGQETDRIWGLRQGAVDYMVKPVSPDQLVAKAQATLAA"
BASE COUNT          220 a          571 c          593 g          239 t
ORIGIN      
        1 tgccgatctc ctggctgctc tcgccgaggc gcttgatgcg cttggaagtc tcctggatcg
       61 tctcgcggat ggtgttcatg ccgtcgatgg tgcgccgcac cgcatcgccc cccttgtggg
      121 cgatctcgac cgagtgccgc gcgacgtccg aggcgcgctc ggcgttgccc gagacctcct
      181 ccaccgagcc cgccatgctg gcggcgctct cggtggcgga gccgatctgc ttcgactggg
      241 cgcccgaggc cttggcgagg tgcgacgaca gcgcctgcgt ctgccgcgtg gcggagtcga
      301 gctggatcgc cgaggcgttg atggtggtga cgaggccgcg cagcgcatcg acggcgtagt
      361 tgatggaatc ggcgatcgcg ccggtgatgt cctcggtgac ggtcgcctgc acggtgagat
      421 ccccgtcggc gaggctcgag agctcgtcca gcagccgcag gatcgcctgc tggttgcggt
      481 cgttctcctg cagccgcgca tccgccgagg cgcgctgccg ctcgaccgtg cgtcgcgtgc
      541 gcagcgccac gaacagcgcg ccgagcagcg ccagcaaggc cgcgccggcg aggaccagcg
      601 gcagccagga aagcggcgcg tgggtcggcg ggtagagcag gtagtcggcg gcgccgccgc
      661 cggcaaccag cagcgccgcg agcgcgagca gtgtcgtcag gctcatgccg ccgcctccgc
      721 gaacgccgga ctctcgagca ccgagcgcag cgagagcacc ggccactgct cgtgcgcgcg
      781 gcggaaggcc ccggcgagat agcgctcgca gcgcgccacc gtcggcggcg cctcacccga
      841 gaactcgctc tcggcgaagc ggcggaaccc cagcacctcg tccaccagca ggcccgcggg
      901 gatctcgcgg tgattcacca ccagcacgcg ggtgttgcgg gtgacgggcg tgacgccgct
      961 gccgaggaac tgccgcaggt cgatgatcgg cagcagctgc ccgcgcacgt tggcgagacc
     1021 acggatccag gccttggcgc ccggcacgcg cgtggtgctc gcgggcacgc cgagcacttc
     1081 gcgggtctcc tcgcgcgcca ccaggtacag gtcaccggcc atgcgcaacg ccaccccgac
     1141 ccactcgcgt ccgctcgcgc cctgctcgct cacctgcgcc gagaccgcac gcccacggcg
     1201 ctcgagctcg gcgagcagct cgaacggccg gtccctgagg gacttgagat cgatctcgga
     1261 gttggttacc ggctcgggca tgggcggggg ttttcgggca cgcggcgagc gcgcggttca
     1321 cgcggcgagt gtcgcctggg ccttggcgac cagctgatcg ggggagacgg gtttcaccat
     1381 gtaatcgacc gcgccctggc gcagccccca gatgcggtcg gtctcctggc ccttggaggt
     1441 gaccatgatg atggggatgg tgcgggtgcg cggatcgttc gccagcgcgc gcgtcgcctg
     1501 gtagccgttc atgcccggca tgacgatatc catgaagatc aggtcgggac tcatctcgcg
     1561 ggccagccgc accccctcgg cgccgtcggc ggcggcggcg gtgcggaagc cgtgcttctc
     1621 gag
//
LOCUS       DATLKL010000592         2092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_18215, whole genome shotgun sequence.
ACCESSION   DATLKL010000592 DATLKL010000000
VERSION     DATLKL010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18215"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VM713_07915"
     CDS             <1..747
                     /locus_tag="VM713_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVH84225.1"
                     /translation="ALATRHPQFADLRAIAAALSSLAGASFGVLAEGANGAGAYLAGA
                     VPHREAGARAVAQSGLTVRDMLQKPLKACVLLGGIEPSIDALDPDSASVFTRTELVVA
                     LTPFASEELKRIAHVMLPIGTFAETSGTYVSCEGLWQSQTGAAAPLGASRPGWKVLRV
                     LGNLLKLPGFEYQSSEDVREELRALCATPASQGYAGEHVVTPAGAASAAVAAGEVRVV
                     DVPMYQTDALVRRAPSLQKTREGRTGPAAY"
     gene            757..1839
                     /gene="nuoH"
                     /locus_tag="VM713_07920"
     CDS             757..1839
                     /gene="nuoH"
                     /locus_tag="VM713_07920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HVH84226.1"
                     /translation="MRNLTYLWSALLDFARSPIGHSSGWIIVITIVLILSVALLTLWE
                     RKVIGWMQLRRGPNRVRIFGILPGVGQPFADVLKLLLKEVVIPANANKLLFRLAPMIA
                     LIPAFAAWAVIPLSPEVVVSKADAGLLYLLSLTSMGVYGVIVAGWAANSKYAFLGAMR
                     SAAQMVAYEIAMGFALVGVLMATGSMNLGTIVRTQEGGPLAWNWLWLFPLLVVYFVSG
                     VAETNRAPFDVAEGESEIVAGFHVEYSGIAFALFFLAEYANMILISALTAVFFFGGWQ
                     GPFQGYPLLGQTFLGQPSFFWFGLKIFLFLFLFLWFRATFPRYRYDQIMRLGWKALIP
                     VTLVWIGVEMLLAVYRVGPWARPWYH"
     gene            1851..>2092
                     /locus_tag="VM713_07925"
     CDS             1851..>2092
                     /locus_tag="VM713_07925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HVH84227.1"
                     /translation="MAINPLKTFLLPELIKGLMVTARTFVSRMYTLNYPEEKTPQSPR
                     FRGLHALRRYPNGQERCIACKLCEAVCPAVCITIDS"
BASE COUNT          298 a          703 c          704 g          387 t
ORIGIN      
        1 gcgctcgcga cccgccatcc gcagttcgcc gacctgcgcg cgatcgccgc cgcgctcagc
       61 tcactcgcag gcgcctcctt cggcgtgctc gccgagggcg cgaacggcgc cggcgcatat
      121 ctcgccgggg ccgtgccgca ccgcgaagcg ggagccaggg cggtggcgca atcgggcctc
      181 acggtgcgcg acatgctgca gaagccgctc aaagcgtgcg tgctgctcgg cggcatcgag
      241 ccctcgatcg atgcgctcga ccccgacagc gcgagcgtgt tcacccgcac cgagctcgtg
      301 gtcgccctga cgccgttcgc gagcgaggag ctcaagcgca tcgcgcacgt gatgttgccg
      361 atcggcacct tcgccgagac ctcggggact tacgtgagct gcgaggggct gtggcagagc
      421 cagactggcg cggcggcgcc gctcggcgcc tcacgcccgg gttggaaggt gctgcgcgtg
      481 ctcggcaatc tcctcaagct tccgggcttc gaataccagt cgtcggagga cgtgcgtgag
      541 gagctgcgag ccctgtgtgc aacacccgcc tcgcagggct acgcgggcga gcacgtcgtg
      601 accccggcgg gagcggcgag cgcggcggtg gccgcaggcg aggtgcgcgt cgtcgacgtg
      661 ccgatgtatc agaccgatgc gttggtgcgc cgggcaccgt cgctgcaaaa gacccgcgaa
      721 ggccgcaccg ggccagcggc gtactgagga cgcacggtgc gcaacctcac ctacctctgg
      781 tccgcgctgc tcgactttgc gcgctcgcca atcggacact cgagcggctg gatcatcgtc
      841 atcaccatcg tgctgatcct cagcgtggcg ctgctcacgc tgtgggagcg caaggtcatc
      901 gggtggatgc agctgcgccg cggacccaac cgcgtgcgca tcttcgggat cctgcccgga
      961 gtcgggcagc cgttcgccga cgtgctgaag ctcctgctga aggaagtggt gatcccggcc
     1021 aacgccaaca agctgctgtt tcgactcgcg ccgatgatcg cgctcatccc ggcgttcgcc
     1081 gcctgggccg tgatcccgct ctcgcccgag gtggtggtgt cgaaggccga cgcgggactc
     1141 ctctatctgc tgtcactcac ctcgatgggc gtgtacggcg tcatcgtcgc cggttgggcg
     1201 gccaactcca agtacgcttt cctcggcgcc atgcgctccg cggcgcagat ggtcgcctac
     1261 gagatcgcca tgggctttgc gctggtcggg gtgctcatgg ccaccggcag catgaacctc
     1321 ggcacgatcg tgcgcactca ggaaggcggc ccgctcgcgt ggaactggct gtggctgttt
     1381 ccgctgctgg tcgtttactt cgtgtcgggt gtcgccgaga ccaatcgcgc gcccttcgat
     1441 gtggcggaag gcgagtcgga gatcgtcgcc ggctttcacg tcgagtattc ggggatcgcc
     1501 ttcgcgctgt ttttcctcgc cgagtacgcc aacatgatcc tgatctcggc gctcaccgcg
     1561 gtattcttct tcggcggctg gcagggcccg ttccagggct atccgctgct cggccagacc
     1621 ttcctaggac agccgagctt cttctggttc gggcttaaga tcttcctgtt cctgttcctg
     1681 tttctctggt tccgcgcgac tttcccgcgc taccgctacg accagatcat gcggctcgga
     1741 tggaaggcgc tgattcccgt gacactggtg tggatcggcg tggagatgct gctggcggtg
     1801 taccgggtcg ggccgtgggc gcggccctgg tatcactagg gactgaaatc gtggctatca
     1861 atccgctcaa gaccttcctg ctgccggagc tcatcaaggg gctgatggtc acggcacgta
     1921 ccttcgtcag ccgcatgtac accctcaact acccggagga gaagacgccg cagtcgccgc
     1981 gcttccgcgg gctgcatgcg ctgcggcgct accccaacgg ccaggagcgc tgcatcgcct
     2041 gcaagctgtg cgaggcggtg tgcccggcgg tgtgcatcac catcgactcg ga
//
LOCUS       DATLKL010000593         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_29700, whole genome shotgun sequence.
ACCESSION   DATLKL010000593 DATLKL010000000
VERSION     DATLKL010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29700"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VM713_07930"
     CDS             complement(<1..655)
                     /locus_tag="VM713_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVH84228.1"
                     /translation="MTLLVTGATGFVMSVLGRHWLDADPDARLVVLDSDPLDAMAQRY
                     FDPVMERMTVVTADVTRPETWRAALAPEGITHIVHGATITPISRGSAAEARREPEAES
                     PARILEVNLMGTVAMLELARTLPHFERFIYVSSGAVYKHHGPDRPGEPLPEDGYVMPR
                     RLYGISKLASELITERYGDLFGLSTASVRPSSVYGTMDRVTRSRSMRHPPNRIAHMAV
                     "
     gene            complement(681..1934)
                     /locus_tag="VM713_07935"
     CDS             complement(681..1934)
                     /locus_tag="VM713_07935"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HVH84229.1"
                     /translation="MSGAPQPDLDLAAAVFEALERATRRGRGIVRDSYGAGEQAAHDI
                     VRAAAGGLGLETAVDALGNLLMTLPGRDRSAPRIIMGSHLDSVPQGGNYDGAAGVVAG
                     LAALSALRRAGVVPRCDLSVMGIRAEESAWFDVAYVGSAGALGMLDPACLAVPRSDNG
                     RSLESTLEQRGFDPRPIRERRRLLDPAGIRAYLELHIEQGPTLVARGLPAAVVSGIRG
                     CKRFRNARCTGEYAHSGAVARRDRHDAVAATVALLHHMEAVWLEQEAAGADLVLTSGE
                     LYTDPALHGPSKIAGEARFVLDIRSLSAATMEAVASEARAAAARIGAAYRVDFDLGAA
                     SDSPAALMDERLRAALMRQLARPFEMASGAGHDAAVFAQAGVPSAMIFVRNEHGSHNA
                     DEAMALEDFAVGTHALLGLLQEFPL"
     gene            1965..>2403
                     /locus_tag="VM713_07940"
     CDS             1965..>2403
                     /locus_tag="VM713_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVH84230.1"
                     /translation="MASLLAGKVAVVTGASSGIGRAIAISFAGEGASVVIADIIGKPL
                     EGGESTLEIIARAGGTASFHRTDVGRSAEIDALIGSTVERYGRLDVMVNNAMTYSGTA
                     LLQTELAQWEEVMRVNLTGMFLGCKRAVQQMISQEPRHEVRGRL"
BASE COUNT          371 a          802 c          878 g          352 t
ORIGIN      
        1 ccacggccat gtgcgcgata cggttgggcg gatggcgcat gctgcggctg cgcgtgacgc
       61 gatccatggt cccgtacacc gaggagggac ggaccgaggc ggtcgatagg ccgaagaggt
      121 cgccgtagcg ctcggtaatg agctccgagg ccagcttcga gatgccatag agccggcgcg
      181 gcattacgta gccgtcctcg ggcagcggct cgccggggcg atccggcccg tgatgcttgt
      241 agactgcgcc cgagctcacg tagatgaatc gctcgaaatg cggcagcgtg cgcgccaact
      301 cgagcatggc gacggtgccc atgagattca cctcgaggat gcgcgcgggg ctctccgcct
      361 cgggctcgcg ccgcgcctcg gcggcgctgc cgcgtgagat gggtgtgatg gtggctccat
      421 ggacgatgtg agtgatgccc tccggggcga gcgccgcgcg ccaggtctcg ggtcgcgtca
      481 cgtcggccgt gacgacggtc atgcgctcca tgaccgggtc gaagtagcgt tgcgccatgg
      541 catccagagg gtcggaatcc agcaccacga ggcgggcgtc ggggtcggcg tcgagccagt
      601 gacggccgag cacgctcatg acgaaacccg tcgcacccgt caccagcaat gtcatttcgg
      661 ctccttaacg ctcggcgggc tcagagtgga aattcctgca acagcccgag cagggcgtgc
      721 gtgccgaccg caaagtcctc gagcgccatg gcctcgtcgg cgttgtggct gccgtgctcg
      781 ttgcgcacga agatcatcgc gctcggcacc ccggcctggg cgaacactgc ggcatcgtgt
      841 ccggcgccgc tcgccatctc gaagggccgg gcgagctggc gcatgagggc cgcgcgcagc
      901 cgctcgtcca tgagtgccgc gggggagtcg ctggccgcgc cgagatcgaa gtcgacgcgg
      961 taggctgcgc cgatgcgcgc ggccgcggcg cgcgcctcgg acgccaccgc ctccatggtc
     1021 gcggcggaga ggctgcgaat gtcgagaacg aagcgggcct cgccggcgat cttgctcggc
     1081 ccgtgcaggg cggggtcggt gtagagctcg cccgaagtca gaaccagatc ggctcccgcg
     1141 gcttcctgct cgagccacac ggcctccatg tgatgaagaa gcgcgaccgt cgccgcgacg
     1201 gcgtcgtgcc ggtcgcgccg ggcgacggcg cccgaatggg cgtattcgcc cgtgcagcgc
     1261 gcgttgcgaa agcgcttgca gccgcggatg ccgctcacca cggcggcagg caggccgcgc
     1321 gcgaccagcg tcggaccctg ctcgatgtgc agctccaggt aggcgcggat gccggcggga
     1381 tccagcaggc gccgccgttc gcggatcggc cgcggatcga agccgcgttg ctcgagcgtc
     1441 gactcgagac tccggccgtt atcggatcgc ggaaccgcga ggcacgcggg atcaagcatt
     1501 ccgagcgcgc cggcactccc cacataagcg acgtcgaacc aggccgactc ttcggcgcgt
     1561 atgcccatca ccgagaggtc gcacctgggg acgacgcccg cgcggcgcag cgccgacagt
     1621 gccgcgagcc cggcgaccac ccccgcggcg ccgtcgtagt tgccgccctg cggaacgctg
     1681 tcgagatgcg agcccatgat gatgcgcggc gcactgcggt cgcgccccgg gagcgtcatg
     1741 aggaggttgc cgagcgcatc gaccgctgtc tcgaggccga gacccccggc cgccgcacgc
     1801 acgatgtcat gcgccgcctg ctcgccggca ccgtagctgt cgcgcacgat gccgcggccg
     1861 cgccgcgtgg cgcgctcgag tgcttcgaag accgcagcag cgaggtcgag gtcgggttgg
     1921 ggcgcgccgg acatccggat attctgctct ctataataga gcgcatggcg agtctactcg
     1981 cggggaaggt tgccgtcgtc accggagcga gctcggggat cggtcgcgcc atcgcgatca
     2041 gcttcgccgg cgagggggcg agcgtcgtca ttgccgatat cattggcaag cccctcgagg
     2101 ggggcgagtc gacgctcgag atcatcgcgc gcgcgggcgg aacggcctcc ttccacagga
     2161 ccgacgtcgg gcggtccgcg gagatcgacg cgctgatcgg cagcaccgtc gagcggtacg
     2221 gccggctcga cgtcatggtg aacaatgcca tgacctattc ggggacggcg ttgcttcaga
     2281 ccgagctcgc gcagtgggag gaggtcatgc gcgtcaatct gacgggcatg tttctcggct
     2341 gcaagcgcgc cgtccagcag atgatctcgc aggagccgcg gcacgaggtc agggggcgcc
     2401 tca
//
LOCUS       DATLKL010000594         2683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_210100, whole genome shotgun sequence.
ACCESSION   DATLKL010000594 DATLKL010000000
VERSION     DATLKL010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210100"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VM713_07945"
     CDS             complement(<1..465)
                     /locus_tag="VM713_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVH84231.1"
                     /translation="MRTLAEPPEAIPGQTTTLVRADPRPDDGTDLHPALRAQLKELRL
                     RTGSSTPDLGMLLKLINDHYHSAERERRGIVESMRLMADEARALADEATEQGSERLQV
                     ILDHIKDVVLALDEDGCIRTFNPTGQRVFGYVEAEVIGQRIDLLLPKLANGET"
     gene            complement(578..1165)
                     /locus_tag="VM713_07950"
     CDS             complement(578..1165)
                     /locus_tag="VM713_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84232.1"
                     /translation="MSQLPRILPLALFSLALGAAGCGSKSTPSATPAKPVAAVKKVAS
                     PADNLSPNLVGAVTTAKSGANLVQVKFELGARPEVGAPVDVDLVLVPQADRIDRISGS
                     VQGDEGLEIVSGATLDPAEKPAFGTPIHHGLKVRAKRDGVFTLRAALTVESGGQTLGP
                     IYSMPLIAGNGLVDAGAGPVAARSAAKPAASAAAK"
     gene            complement(1195..2199)
                     /locus_tag="VM713_07955"
     CDS             complement(1195..2199)
                     /locus_tag="VM713_07955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HVH84233.1"
                     /translation="MIRKSSGSDLQPFNGLTPAVVLDAAAAAGLDTDGRLFALNSYEN
                     RVYQLHGAHGPLVLKFYRPARWSDAQIAEEHQFTAELAAAELPVAAPLQLAGTTLARY
                     LGFRFACFPWLRGRAPELDAPEARALLGRTLARLHQVGARRVFEVRPHIGVERLGWQA
                     RAQVLACELLPEALAERYSSVSGALLERVVAGFAAAGSLAEIRLHGDCHLGNLLWNEH
                     GPAFVDLDDCAMGPRVQDLWMMLSGSPAEQQQQWGEILEGYRQFADFDLRELALIEPL
                     RALRMLHHAAWVAHRWSDPAFPRAFPWFGEPRYWEGYLQDLLEQIEAIDDPPLLRGSP
                     "
     gene            2241..>2683
                     /locus_tag="VM713_07960"
     CDS             2241..>2683
                     /locus_tag="VM713_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVH84234.1"
                     /translation="MEHGSILVTGGAGYIGSHVVLQLRMRGERVVVLDDLSRGFRRAV
                     LDAPLVVGRVGDRDAVLALLREHAVDTVMHFAAYTIAPESVREPLKYYGNNTCSTRTL
                     LECCLEAGVEHFVFSSTAAVYGMPADGFASETTATAPINAYGTSKL"
BASE COUNT          421 a          916 c          912 g          434 t
ORIGIN      
        1 ggtctcgccg ttggcgagct tcggcagcag taggtcgatc cgctgtccga tgacctccgc
       61 ttccacgtag ccgaatacgc gctgcccggt cgggttgaag gtgcggatgc agccgtcctc
      121 gtcgagtgcg agcacgacgt ccttgatgtg atcgagaatg acctgcaggc gctccgagcc
      181 ctgctcggtc gcctcgtccg ccagcgcccg cgcttcatcg gccatgaggc gcatcgactc
      241 gacgatcccg cggcgctcgc gctcggcgct gtgatagtga tcgttgatga gtttcagcag
      301 catcccgaga tcgggggtgc tgctcccggt ccgcagccgc agctccttga gctgggcacg
      361 cagcgccggg tgcagatccg tcccgtcgtc ggggcgcggg tccgcccgaa cgagtgtcgt
      421 ggtttgaccg gggatcgcct ccgggggctc agcgagcgtt cgcatggtcc gggtttttga
      481 cataatgaag tcgactcgac ccgatccaga tcatacgagc ggcttctcgg gcggcccgtg
      541 aagcctctcc cgattctgat gggttccttc gacgctttta tttcgccgcg gcgctcgccg
      601 ccggctttgc tgcactgcgg gccgctaccg gccccgcccc ggcatcgacc agaccgtttc
      661 cggcgatgag cggcatcgag tagatggggc cgagggtctg gccgccggac tcaaccgtga
      721 gcgccgcgcg cagagtgaag actccatcgc gtttggcccg caccttcagt ccgtggtgaa
      781 tcggcgtgcc gaaggctggc ttttccgcgg ggtcgagggt cgcaccgctc acgatctcga
      841 ggccctcatc accctggacc gagcccgaga tccggtcgat gcgatccgcc tgcggcacga
      901 gaaccaggtc cacgtccacc ggagcgccga cctcggggcg cgccccaagc tcgaacttca
      961 cctgcaccag gttggccccg ctcttggcgg tggtcaccgc gccgaccaga ttcggagaga
     1021 ggttgtcggc cggacttgcc accttcttga ccgctgcgac cggcttggcc ggtgtggcgc
     1081 tgggcgtgct cttggagccg cagccggccg ccccaagggc gaggctgaag agcgcgagag
     1141 gcagaattcg cggtagctgg ctcatgagct cggcacgtta cctgaggaac gggctcaggg
     1201 ggagccccgc aggagcggcg gatcatcgat cgcctcgatc tgctcgagca ggtcctgcag
     1261 atatccctcc cagtagcggg gctcgccgaa ccaggggaaa gcgcggggaa aggccggatc
     1321 gctccagcga tgcgcgaccc acgcagcgtg gtgcagcatc cgcagggccc gcagcggctc
     1381 gatgagggcg agctcgcgca gatcgaaatc cgcgaactgc cgatagccct ccaggatctc
     1441 gccccactgc tgctgctgct ccgcgggcga gcctgacaac atcatccaca ggtcctgcac
     1501 ccgtgggccc atcgcgcagt cgtcgagatc gacgaaggcc ggcccgtgct cgttccagag
     1561 gagattcccc agatgacagt cgccgtgcag gcggatctcg gcgaggctgc cggccgcggc
     1621 gaagcctgcc accacgcgct cgagcagcgc gccgctcacc gaggagtagc gctcggcgag
     1681 cgcctcgggc agcagctcgc aggcgagcac ctgcgcgcgc gcctgccatc cgagccgctc
     1741 gacgccgatg tgcgggcgca cttcgaagac gcggcgtgca cccacctgat gcaatcgcgc
     1801 gagcgttctg ccaaggagcg ctcgcgcctc cggcgcatcg agctccggag cgcggccgcg
     1861 cagccaggga aagcaggcga agcggaaccc gaggtaacgg gcgagcgtcg tgccggcgag
     1921 ttgcagcgga gctgcgaccg ggagctcggc agcggccagc tccgccgtga actgatgctc
     1981 ctcggcgatc tgcgcatcgc tccagcgggc cgggcggtag aacttgagca ccagcggccc
     2041 gtgagccccg tgcagctgat agacacggtt ctcgtagcta ttgagggcaa agagcctccc
     2101 gtcggtatcg aggccggccg cggcggccgc atcgagaacc accgcgggag tcaaaccgtt
     2161 gaacggctgc aggtcgctgc cggagctttt gcgtatcatc gctgcccttg cctcacgagc
     2221 gatcgattca agctaagccc atggaacacg gcagcatatt ggtgacgggg ggtgcaggct
     2281 acatcggcag tcacgtggtg ctgcagctgc gcatgcgcgg cgagcgcgtg gtggtgctcg
     2341 atgacctgtc gcggggtttc cgccgcgcgg tgctggacgc gccgctcgtc gtgggccgcg
     2401 tcggcgaccg cgacgcggtg ctcgcgctgc tgcgcgagca cgcggtcgat acggtgatgc
     2461 acttcgccgc ctacaccatc gcgccagaat cggtacgcga gccgctcaag tactacggca
     2521 acaacacctg ctcgacgcgc accctcctcg agtgctgcct cgaggcggga gtcgaacact
     2581 tcgttttctc ctcgaccgcg gcggtgtacg gcatgcccgc cgacggcttc gcctccgaga
     2641 cgaccgccac ggcgccgatc aatgcctacg gcacctccaa gct
//
LOCUS       DATLKL010000595         2416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_221251, whole genome shotgun sequence.
ACCESSION   DATLKL010000595 DATLKL010000000
VERSION     DATLKL010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221251"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2235)
                     /locus_tag="VM713_07965"
     CDS             complement(<1..2235)
                     /locus_tag="VM713_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HVH84235.1"
                     /translation="MSAAPGGPGLAPTWSSSNKEMVGCPLGPARLWFTIGGGIVNEVY
                     YPRVDIPQIRDLGFIVADGAGFWVEVKRMWRHDLELADAGVPAVTIVHHHARFELTLR
                     VTPCEDRDVLLIEVTLTGDAALRPYALLAPHLGGTGLGNRAEVVSYRGRKVLWAEQGP
                     YALALAAATPGQADAFVRGSAGYAGENDGWQDFFRHGAMTWEFEAAGPGNVALLGELP
                     RQAVLALGFGSNADSAATLALTALGEPFEANWTRQRQAWVRWHATGTPVDSLPADLPV
                     EILTLFRTSTMVLRSHRDKTYPGAMVASLSVPWGNTHEEREGYHLVWPRDLVECAGAL
                     LAVGATTEARNTLLYLLATQNADGHWNQNQWLGGKGYWSGIQLDEAAFPVLLAAALGE
                     RNALEGTEVSDMVRRALAFLVRTGPVSDQDRWEEDAGLNTFTLAVCIAALVAGAPHLP
                     VEARDLALAFADYWNARLEDWTAVADTPPAQRYGIPGYYVRVAPPEALTDRCCPLNLV
                     LAIKNRAKDPGLPVSEQVGVDFLQLVRFGLRRADDPLILATLHLVDALLKVDTPEGPS
                     WHRYNDDGYGEHEDGAPYDGTGRGRAWPLLTGERGHYEIAAGRDPLPLIAAMARMASS
                     GGMLPEQVWDGAPIPALGLEPGRPTGGAMPLAWTHAEYIKLVVSRGLGRPLDRPPLVW
                     ERYRGERPPLRRVIWCEHAPASELPEGCSLTLALREAGTFRYGFDGWQNAREQSTTAH
                     ALGLH"
     gene            complement(2232..>2416)
                     /locus_tag="VM713_07970"
     CDS             complement(2232..>2416)
                     /locus_tag="VM713_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84236.1"
                     /translation="AVGDVVAHLRAALEADYVVLGGGNAARLKKLPPGARRGENRNAF
                     IGGVRIWRQDGGTLRL"
BASE COUNT          356 a          857 c          862 g          341 t
ORIGIN      
        1 atgcagcccg agggcgtggg cggtcgttga ctgctcgcgg gcgttctgcc agccgtcgaa
       61 gccgtagcgg aaagtgccgg cctcgcgcag cgcgagcgtg agcgagcagc cctccggcag
      121 ctcgctggcc ggcgcgtgct cgcaccagat cacccgccgg aggggcggcc gctcgccacg
      181 gtagcgctcc cacacgagtg gcggccggtc gagcgggcgt ccgaggccgc gcgacacaac
      241 cagtttgatg tactcggcgt gcgtccaggc gagcggcatt gccccgcccg tagggcgtcc
      301 gggctcgagt ccgagggcgg ggatcggcgc gccgtcccac acctgctcag ggagcatgcc
      361 gcccgatgaa gccatgcgcg ccatggccgc gatgagcggc aacgggtcgc gcccggcggc
      421 aatctcgtag tgaccgcgct cgcccgtgag cagcggccaa gcgcgaccgc gcccggtgcc
      481 atcgtacggg gcgccgtcct cgtgctcgcc gtatccgtcg tcgttgtatc ggtgccagga
      541 ggggccctcg ggtgtgtcga ccttgaggag cgcatccacc agatgcaagg tcgcgaggat
      601 cagcggatca tcggcgcggc gcaacccgaa gcgcaccagc tgcaggaaat cgacgccgac
      661 ctgctcggac accggaagtc ccgggtcctt ggcccggttc ttgatcgcca gcaccaggtt
      721 cagtgggcag cagcggtcgg taagcgcctc gggcggcgcg acccgcacgt agtaacccgg
      781 gatgccgtag cgctgcgcgg gcggcgtgtc ggccaccgcg gtccagtcct ccaggcgtgc
      841 gttccaatag tcggcgaaag cgagcgccag atcgcgcgcc tccacgggca ggtgcggtgc
      901 tccggcgacg agcgcggcga tacagactgc cagcgtgaag gtgttgagac ccgcgtcctc
      961 ctcccagcgg tcctgatcgc tcaccggtcc ggtgcgcacg aggaaggcga gtgcgcggcg
     1021 caccatgtcg ctgacctcgg tgccctcgag cgcattgcgc tcgccgagcg ccgccgcgag
     1081 cagcacggga aacgcggcct catcgagctg gattccggac cagtagccct tgccgccgag
     1141 ccactggttc tgattccagt gtccgtcggc attctgcgtc gcgagcaggt aaagcagggt
     1201 gttgcgcgcc tcggtggtgg cgccgaccgc gagcagcgcg cccgcgcact ccaccaggtc
     1261 gcgtggccat accaggtggt agccctcgcg ctcctcatgc gtgttgcccc acggcacgct
     1321 gagcgaggcc accatggcgc cgggataggt cttgtcgcgg tggctgcgca gcaccatcgt
     1381 ggaggtgcgg aacagcgtga ggatctcgac gggcagatca gcgggcagcg agtcgacggg
     1441 cgtgccggtc gcgtgccagc gcacccaggc ttgccgctga cgggtccagt tcgcctcgaa
     1501 gggctcgccg agcgcggtga gtgccagcgt ggcggccgaa tcggcgttgc tgccgaagcc
     1561 gagcgcgagc accgcctgcc gcggcagctc gccgagcagc gccacgttgc ccggacccgc
     1621 cgcctcgaat tcccaggtca ttgcgccgtg acggaagaag tcctgccagc cgtcgttctc
     1681 gcccgcgtag ccggcgctgc cgcgcacgaa ggcatccgcc tgcccgggag tggcggcagc
     1741 gagcgccaga gcgtagggac cctgctcggc ccacaacact ttgcgcccgc ggtagctgac
     1801 cacctcggcg cggttgccga gtcccgtgcc gccgaggtgc ggcgcgagca gcgcgtaggg
     1861 cctgagcgcc gcatctcccg tgagtgtcac ctcgatgagg agcacgtcgc gatcctcgca
     1921 cggcgtgact cgcagcgtga gctcgaagcg cgcgtggtgg tgaacgatcg tcacggccgg
     1981 cacgcccgcg tccgcgagct cgaggtcgtg gcgccacatg cgcttgacct ccacccagaa
     2041 cccggcgccg tcggcgacga tgaagccgag atcgcgaatc tgcggaatgt cgacgcgcgg
     2101 gtagtagacc tcgttgacga tgccgccgcc gatcgtgaac cacaggcgcg cagggccgag
     2161 cggacacccg accatctcct tgttgctgct gctccaggtg ggagcgagcc ccgggccacc
     2221 cggtgcggcg ctcatagtcg cagggtcccg ccgtcctgcc gccagatgcg cacgccgccg
     2281 atgaaggcgt tgcggttctc gccacgccgc gcaccgggcg gaagcttctt cagtcgcgcc
     2341 gcattgccgc ccccgagcac cacgtagtcg gcctcgaggg ccgcgcgcag gtgcgccacc
     2401 acatcgccga ccgccg
//
LOCUS       DATLKL010000596         2538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_221268, whole genome shotgun sequence.
ACCESSION   DATLKL010000596 DATLKL010000000
VERSION     DATLKL010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221268"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /locus_tag="VM713_07975"
     CDS             <1..1559
                     /locus_tag="VM713_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVH84237.1"
                     /translation="LLQQNPEIRGLRASTMRAVARNLWLIDEEFRQNPRHHRLFLEIL
                     RSPVGVTHELRRMNTYGVLGRYIPAFGRVVGRMQYDLFHAYTVDAHTLFVVSNLRRFA
                     IPRYDHELPEASRVMQQLPRQEIVYLAALFHDLAKGRGGDHSELGAVDAEAFCLEQGL
                     SPYDARLVAWLVRNHLELSITSQKQDIGDPQVINAFARKVGDETHLDHLYVLTCADVR
                     GTNPKLWNSWKASLFKDFYQRVKRALRRGLESPIDPEHLVRETQDAARRLLIERGVGE
                     RDIAASWKRFSPGYFLQHSPEDVAWHTRLLAERDAASDEPLVALDPHSLRGTTAVLIF
                     TRPRRHGFARTTAVLDQLGLNIVDARITPTGDGFSLDLYHVLEDDGAGVTDNDRQVEI
                     EQALWRSVQGPGGAPLSVSRRAPRQARMFNTPTQIALSIDERNRRSVLELTAGDRPGL
                     LCDVGKVLVEERVELHAAKIMTVGERAEDVLYLTDFDNRPLSGEASERLKQRLVAALD
                     REQQALRATP"
     gene            1556..2395
                     /gene="dapD"
                     /locus_tag="VM713_07980"
     CDS             1556..2395
                     /gene="dapD"
                     /locus_tag="VM713_07980"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HVH84238.1"
                     /translation="MSAAPELRTAIDAAFERRAELTPENLPPALAAALEQCLALLDSG
                     QARVAEPRDGGWVVNEWLKKAVLLYFRTHDNRVIDAGFTRFFDKVPLKYAGASEAELR
                     AGGARVVPHAIVRRGAYIAPDAVLMPSYVNIGAYVGAGTMVDTWATVGSCAQIGRNVH
                     LSGGVGIGGVLEPLQASPTIIEDDCFIGARSEVVEGVVVGAGSVISMGVFIGQSTRIY
                     DRTTGTVLQGRVPPGSVVVSGTLPSADGRYGLYCAVIVKRVDAQTRAKTSINELLRSA
                     AER"
BASE COUNT          383 a          896 c          881 g          378 t
ORIGIN      
        1 cgctgctgca gcagaatccg gagatccgcg gcctgcgcgc ctcgacgatg cgcgcggtgg
       61 cgaggaatct ctggctcatc gacgaggagt tccgccagaa cccgcgccat caccgcctgt
      121 tcctcgagat cctccgctcg ccggtcggag tgacccacga gctgcggcgc atgaacacct
      181 acggcgtgct ggggcgctac atcccggcct tcgggcgcgt cgtcgggcgc atgcagtacg
      241 acctgttcca cgcctacacg gtcgacgccc acacgctgtt cgtggtgagc aatctgcggc
      301 gcttcgccat cccgcgctac gaccacgagc ttcccgaagc ctcgcgcgtc atgcagcagc
      361 tgccgcgcca ggagatcgtg tacctcgcgg cgctgtttca cgacctcgcc aagggacgcg
      421 gcggcgatca ttccgagctc ggcgcggtgg acgccgaagc cttctgcctc gagcagggcc
      481 tctcgcccta cgatgcgcgc ctcgtcgcct ggctggtgcg caatcacctc gagctctcga
      541 tcacctccca gaagcaggac atcggcgacc cgcaggtgat caacgccttc gcgcgcaagg
      601 tcggcgacga gacgcacctc gaccacctct acgtgctcac ctgcgcggac gtgcgcggca
      661 cgaacccgaa gctgtggaac tcctggaagg cctcgctctt caaggacttc taccagcgtg
      721 tcaagcgcgc gctgcgccgc gggctcgaga gcccgattga tccggagcat ctggtgcgcg
      781 agacgcagga cgcggcgcgg cggctgctga tcgagcgcgg cgtcggcgag cgcgacatcg
      841 ccgcaagctg gaagcgcttc tcgcccggat acttcctgca gcactcgccc gaggacgtcg
      901 cctggcacac gcgcttgctc gcggagcgcg atgcggcctc cgacgagccg ctggtagcgc
      961 tcgatccgca cagcttgcgc ggcaccaccg cggtgctgat cttcacgcgc ccgcgccggc
     1021 atggcttcgc gcgcaccacc gcggtgctcg atcagctcgg gctcaacatc gtcgacgcgc
     1081 gcatcacccc gaccggcgac ggcttcagcc tcgacctcta ccacgtgctc gaggacgacg
     1141 gcgctggcgt caccgacaac gaccgccagg tcgagatcga gcaggcgctg tggcgctcgg
     1201 tccagggtcc cgggggtgcg ccgctctcgg tgtcgcggcg tgccccgcgc caggcgcgca
     1261 tgttcaacac cccgacgcag atcgccctca gcatcgatga gcgcaaccgc cgctcggtgc
     1321 tcgagctcac tgccggcgac cggccggggc tgctgtgcga cgtcggcaag gtgctggtcg
     1381 aggagcgcgt cgagctgcac gcggccaaga tcatgacggt cggcgagcgg gccgaggacg
     1441 tgctctacct caccgacttc gacaaccggc cgctgagcgg cgaggccagt gaacggctga
     1501 agcagcggct ggtcgccgcg ctcgatcgcg agcagcaggc gctgcgggcg acaccgtgag
     1561 cgcggcgccc gagctacgca cggccatcga cgccgccttc gagcgacgcg cggagctcac
     1621 gcctgagaat ctgccgcccg cgctcgcggc ggcgctcgag cagtgcctcg cgctgctcga
     1681 cagcgggcag gcgcgggtcg ccgagccgcg tgacggcggc tgggtggtga atgagtggct
     1741 gaagaaggcg gtactccttt atttccgcac gcacgacaac cgcgtgatcg atgccggctt
     1801 cacccgcttc ttcgacaagg tgccgctcaa atacgcgggc gcgagcgagg ccgagctgcg
     1861 cgcgggcggc gcgcgcgtcg tgccgcatgc gatcgtacgc cgcggcgcct acatcgcccc
     1921 cgacgcggta ttgatgccga gctacgtgaa catcggtgcc tacgtgggcg ccggcacgat
     1981 ggtggacacc tgggccaccg tgggctcgtg cgcgcagatc ggccgcaacg tgcacctctc
     2041 gggcggcgtc gggatcggcg gcgtgctcga gccgttgcag gcgagcccga cgatcatcga
     2101 ggacgactgc ttcatcggag cgcgttccga ggtcgtcgag ggagtcgtgg tgggggctgg
     2161 ctcggtcatc tccatgggcg tgttcatcgg ccagagcacc cgtatctacg atcgcacgac
     2221 gggcacggtc ctgcaggggc gtgtcccgcc gggatcggtg gtggtgtcgg gcacgctgcc
     2281 ctcggccgac gggcgctacg gcctgtactg cgcggtcatc gtcaaacgcg tcgatgcgca
     2341 gacgcgcgcc aagacctcga tcaacgagct gttgaggagc gccgcagagc gctgaaggca
     2401 gcagcccaat cagctgccct ggcgatcctt gagccgcacg accggggcgc cgcgtcggcg
     2461 gcggcgcggg ctgtccgatg gcgctggccg tgtgagatcc gcgatcgacc tgaaggtgcg
     2521 cgcagcgcgc cgctgcag
//
LOCUS       DATLKL010000597         2277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_29767, whole genome shotgun sequence.
ACCESSION   DATLKL010000597 DATLKL010000000
VERSION     DATLKL010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29767"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VM713_07985"
     CDS             <1..232
                     /locus_tag="VM713_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH84239.1"
                     /translation="HVDILFTDVVMPGGMTGVDLARAARQRRPKLGVLFTSGYAELAD
                     IRKAMMTENAVWLAKPYTNSDLAAKLREIVDH"
     gene            complement(241..1029)
                     /locus_tag="VM713_07990"
     CDS             complement(241..1029)
                     /locus_tag="VM713_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH84240.1"
                     /translation="MFPLAPATSCAMSNQARTGPPHILVVDDDPRVRSMLRRYLTDEG
                     FRVSDVSNGAAMRVALEHGDIALVLLDLVIPGEDGLALARHIRHRSDIPIIMLTGKGE
                     LIDRVVGLEAGADDYISKPFHLREVLARIRTVLRRASLKPAPAAAAEAVATVPDRETI
                     AFQGWRLDLVRRELLNPAGAPVPLTTAEFELLRAFARNANRVLSRDRLLDLVKGRAWS
                     AYDRSIDTQVVRLRRKIERDPSKPELIKTVRGGGYIFTVAVNSG"
     gene            1282..1989
                     /locus_tag="VM713_07995"
     CDS             1282..1989
                     /locus_tag="VM713_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVH84241.1"
                     /translation="MALNPLRQVKAHHTLFYEGDPADHVYEVRKGTITLYKLLPDGRR
                     QVLGFLSAGHLLGLSRDGHYIHTAEAVTDVALIRYPRVQFERLVKEAPAFASRIFAAT
                     SDELRAAQDQMLLLGRKTADEKMASFLLMMAERQEPGQGAVHIPMGRSDIADYLGLTT
                     ETVCRTFSRFRRDGLIRLSDPSRVELLNRERLEELASGETGNKTTVTIRPRSAPAASR
                     QISGSLRATSSSATLRR"
BASE COUNT          422 a          741 c          726 g          388 t
ORIGIN      
        1 tcacgtcgac atcctgttca ccgatgtcgt catgccgggc ggcatgacgg gggtcgatct
       61 tgcgcgcgcg gcgcgccagc gccggcccaa gctcggggtt ctcttcacgt ccggctacgc
      121 ggagctggcc gatatcagga aggccatgat gaccgagaac gcggtttggc tcgcgaagcc
      181 ctacaccaac agcgatctcg cggccaagct gcgcgagatc gtcgatcatt agtcgcgcgc
      241 tcagccgctg ttcacggcga cggtaaagat gtacccgccg ccccgcaccg tcttgatcag
      301 ctctggcttg ctcgggtcgc gctcgatctt gcggcggagg cgcacgacct gcgtgtcgat
      361 gctccggtca tatgccgacc acgcgcgacc cttcacgaga tcgaggagtc gatcccggct
      421 gagcacgcgg ttggcattgc gggcaaaggc gcgaagcagc tcgaactctg cggtcgtgag
      481 cggaaccggc gcgcctgctg ggttgagcag ctccctcctc acgaggtcga gcctccagcc
      541 ctggaaggcg atcgtctctc gatcggggac agttgccacg gcctccgcgg cggcggccgg
      601 cgccggcttg agggatgcgc ggcggagcac ggtgcggatg cgcgccaaaa cctcccgcaa
      661 gtggaagggc ttgctgatgt aatcgtcggc cccggcctcg agtccgacga cccgatcgat
      721 cagctcgccc ttgccagtca gcataataat gggaatgtcc gagcgatggc ggatgtgccg
      781 ggccagcgcg aggccatcct caccagggat cacgagatcg aggagcacga gtgcgatatc
      841 accgtgctcg agcgccacgc gcatggcggc gccgtttgac acgtcgctta cgcggaaccc
      901 ctcgtccgtc aaatagcggc gcaacatcga ccggacgcgc ggatcgtcgt cgacgaccag
      961 aatgtggggc gggccagttc gagcctggtt cgacatggca cacgaggtag cgggggccag
     1021 gggaaacatc cccaggagcc tctgtcacaa accgtcacaa ttcgtctcgg actgatacca
     1081 gattccgctt ggattggcat catcctggcg aatctggtgg caatgagacg gtattcattt
     1141 ggcgcatcgg cgaacgcgag cgccgaagaa gagaggaact ctcccgtgta cggaactttg
     1201 gcctcatccc gcgccgcgag cccagatcgt gcggcgccgc ccgccctggg cgggctccag
     1261 cgcactgccg ctgcgcaagt cgtggctctc aacccgctcc ggcaagtcaa ggcccatcat
     1321 accctcttct acgaggggga cccggccgat catgtttacg aggtcaggaa aggcacgatc
     1381 acgctctaca agctcctgcc cgacgggcgg cgacaggtgt taggattctt gagcgccgga
     1441 catctccttg gcctctctcg cgatgggcat tacattcaca ccgccgaggc ggtgaccgac
     1501 gtcgcgctga tccgctaccc gcgagtgcaa ttcgagcggc tggtcaaaga agcgcccgcc
     1561 ttcgcgagcc ggattttcgc cgccacctcc gacgagcttc gcgcagcgca ggatcagatg
     1621 ctgcttctcg gacgcaagac ggccgatgag aaaatggcga gcttcctcct catgatggcg
     1681 gagcgtcagg agccgggcca gggcgccgtg cacattccga tgggacgaag cgacatcgcg
     1741 gactacctcg gcctaaccac cgagacggtc tgccggacct tcagcaggtt caggcgtgac
     1801 gggctgatca gactatccga tccatcgcgg gtcgagctac tcaaccggga gcggctcgag
     1861 gagctcgcct ccggcgagac gggaaacaag accacagtaa cgatcagacc ccgcagcgcg
     1921 ccagcggcct cgaggcagat ctcgggatca ttgagggcga cctcgagctc ggccaccttg
     1981 cggcggtaga catcggcgag attggggtga agggcaggta caggtcccgg atcgtcctcg
     2041 aattcgaggc gctgaatgca ggcatcgcac ttggcctcga gctcgagcaa ccgcgtcttc
     2101 gtcgtcgccg tgacgatccc ttgctcgatg gcggctcgtt cgccgaacgt ttgactggag
     2161 caaccccttc accatggcgg cgtcacggtc gtccagttcg ggccccttag acgaaggtat
     2221 gcgcgctcca atacctccca taacccgacc gctacccgcg tgcatgcgat ctatact
//
LOCUS       DATLKL010000598         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_97632, whole genome shotgun sequence.
ACCESSION   DATLKL010000598 DATLKL010000000
VERSION     DATLKL010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97632"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1574
                     /locus_tag="VM713_08000"
     CDS             234..1574
                     /locus_tag="VM713_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84242.1"
                     /translation="MNSSVTRLRLLARQLAGVALTLACAGELAGAAATAGGDFESVPD
                     RAPGEVLPAALVSGPDFQVAAPVHGDGLMNHFVLDSRFGEFDAYGRAALAIRIREVAA
                     LSELARTSDVQIAAGGVVQGVEVEVKTATGVVTHPVQTVSGIPRGIAHLFSGYKAEGE
                     EVAADARSSLASSGSASGSSLRSTAEKGGRAAKSYAERYLGITAAERDYYRKLGVDPY
                     TDNKVLREAIRRDARIAAGAGFGVRFVGLPGIPGIGIAQRAVDAIYNEDPAVIRERTR
                     TTLAGLGLSTVEIERFMNAPLLSPTRQLVLLAAAQALGGVTQRGELFRHAIGLSSDEE
                     VQVYLRSAGLLAKVHADRPVASVVAGVRLPAALRADGSVVVCGAFESVYWTEAVSHLD
                     EQLRAALPPQPPGRARELWVAGILSDPARGAAHERGWELHEVPDEAPAPTPSGR"
     gene            1679..2026
                     /locus_tag="VM713_08005"
     CDS             1679..2026
                     /locus_tag="VM713_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84243.1"
                     /translation="MWLIIAIFAVAAVVGLTMAIAAFQGKFPPVPSAILHGVLAATGL
                     VLLLVAVFVKGAAGAALWALGFFLVAALGGFTLALGFHARRKPLPQGFVAGHALLAVI
                     GFLLLLAGALKLI"
     gene            2038..2478
                     /locus_tag="VM713_08010"
     CDS             2038..2478
                     /locus_tag="VM713_08010"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HVH84244.1"
                     /translation="MRSSSFVHPVHPALAHFPLAFWLGSCASDLIALKTGNAVWWTVS
                     HYALAAGAIMGAVTVLAGGLELWLRKLPAAAWRWAGLHAGLMVTALLCFMVSLSWRAA
                     TPPPISAVWVSLLGAALVIAGGFCGGTLVYGYGVGVSWRAERGE"
     gene            2541..>2887
                     /locus_tag="VM713_08015"
     CDS             2541..>2887
                     /locus_tag="VM713_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVH84245.1"
                     /translation="MPAAVLQALKRGGPRTIRDLAAALDITYEAVRQQITELVRAGWV
                     EGSSAPAAAHRVPAGPPEPKPGPASRRYRLSTAAEHLFPKHYDELSLELVQHVLARFG
                     GAGLVEILGRMTEA"
BASE COUNT          389 a          993 c         1030 g          475 t
ORIGIN      
        1 cggaagattc ccgtagtctt caaaatgcca tcgttcaaaa tgcaatgtgc gtgccatgac
       61 gaatccgcgc cgaccgcggc gacggcgcgg cgcgtgcctg gttccggggc agtcgcgctg
      121 gcgcagcacg cccgtggtgc acgccgcgcc gcttgcgttg acactctact ttcgcgggcc
      181 ccatgatcgg gcgctcgagc gatgccaccg ccgcacgaca caaagcaggt tcgttgaatt
      241 caagcgtcac gcgtctgcga ctgctggcgc ggcagctggc cggggttgcg ctgacgctgg
      301 cctgcgcggg cgagctggca ggcgccgcgg ccaccgccgg cggtgatttc gagtccgtgc
      361 cggaccgcgc gcccggggag gtgctgcccg cggcgctggt atcgggcccg gacttccagg
      421 tcgcggcgcc ggtgcacggc gacggactga tgaatcactt cgtgctcgac agccgcttcg
      481 gcgagttcga cgcctacggc cgggcggcgc tcgcgattcg catccgcgag gtcgccgccc
      541 tcagtgagct cgcgagaacc tccgacgtgc agattgccgc cggcggcgtg gtgcagggag
      601 tcgaagtgga ggtaaagacg gcgaccggtg tcgtgaccca cccggtgcag accgtcagcg
      661 gaattcccag gggcattgcc caccttttca gcggctacaa ggcggagggc gaggaggtcg
      721 cagcggacgc ccgcagcagc ctcgcgtcct cgggctccgc gagcggcagt tccctccgca
      781 gcaccgccga gaagggcgga cgggcggcga agagctacgc cgagcgctat ctcggcatca
      841 ccgccgccga gcgcgactac taccgcaagc tcggcgtcga cccctacacg gacaacaagg
      901 tgctgcgtga ggcgatcagg cgcgatgcgc gaatcgccgc cggcgcgggc ttcggcgtga
      961 ggttcgtagg tctcccggga attccgggaa tcggcatcgc gcagcgcgcg gtcgatgcga
     1021 tctataacga ggacccggcc gtaatccgcg agcgcacgcg cacgacactc gctggtctcg
     1081 gactgagcac ggtggaaatc gagcgcttca tgaacgcgcc gctcctgagt cccactcggc
     1141 agctggtatt gctcgcggct gcgcaggcgc tcggcggcgt gacgcagcgg ggcgagctgt
     1201 ttcgccatgc catcgggctg agttcggacg aggaggtcca ggtttacctg cgcagcgccg
     1261 gactccttgc caaggtacac gccgaccgcc ccgtcgcctc tgtggtggcc ggggtgcggc
     1321 tgccggcggc gctgcgcgcc gacgggagcg tcgtggtctg cggcgccttc gagtccgtct
     1381 actggaccga ggccgtgtcg cacctggacg agcagctgcg cgccgcattg ccgccgcagc
     1441 cgccgggcag ggcgcgcgag ctctgggtgg ccggaatact ttccgaccct gcgcggggcg
     1501 cggcacacga acgcggctgg gagctgcacg aagtgccgga tgaggcaccc gcgccgaccc
     1561 cctccggccg ctgagcagca accgccggga acgcccgcgc ccgtccggag cttgcagcgg
     1621 gcggcgcacg gggctataag caatatacaa gtacttactt gtatgtcagg acaggcttat
     1681 gtggctcatc attgcgatct ttgccgtcgc cgccgtcgtg ggtctcacca tggccatcgc
     1741 ggctttccag gggaagttcc cgccggtgcc gagcgcgatc ctgcacggcg tcctcgccgc
     1801 gaccggcctc gtgctgctgc tcgtggcggt gttcgtcaag ggagcggccg gagcggcgct
     1861 ctgggcgctc ggtttctttc tggtcgcggc gctcggtggc tttacgctcg ccctggggtt
     1921 ccacgcccgc aggaagccgc tgccgcaggg cttcgtcgcc ggccacgcgc ttcttgccgt
     1981 gatcggcttt ctgctgctcc tcgctggcgc gctgaagctc atctgacctg agcctccatg
     2041 aggagctcat cgtttgtgca cccggtgcat ccggcgctcg cgcatttccc gctcgccttc
     2101 tggctcggct cgtgcgcctc ggatctcatc gcgctcaaga cgggcaacgc cgtgtggtgg
     2161 accgtgagcc actacgccct ggccgcgggc gcgatcatgg gcgcggtcac cgtgctcgcc
     2221 ggcgggctcg agctgtggct gcgtaagctc ccagccgcag cctggcgatg ggcggggctg
     2281 catgcggggc tcatggtcac ggcgctgctc tgcttcatgg tgagcctgtc gtggcgcgcc
     2341 gcaacgccgc cgccaatctc cgcggtgtgg gtctcgctgc tcggtgcggc gctggtcatc
     2401 gccggcggat tctgtggcgg cacgctcgtg tacggttacg gggtcggagt ttcgtggcgc
     2461 gccgaaagag gcgagtagca gccgcgcgcg ggcggcgcgc cgcgccgcgt gcctccgcgc
     2521 agcgcccggc agcccgcgcg gtgcctgcgg cggtccttca ggcgttgaag cgcggaggtc
     2581 ccaggaccat ccgcgatctt gccgcggccc tcgacatcac ctacgaagcc gtcagacagc
     2641 agatcaccga gctcgtgcgc gcgggctggg ttgagggcag cagtgcgccg gccgccgcac
     2701 atcgcgtccc ggccggcccg cccgagccaa agccgggtcc cgcttcgcgg cgctaccggc
     2761 tgagcaccgc cgcggagcat cttttcccca agcactacga cgagctctca ctcgagctcg
     2821 ttcagcatgt gctcgcgcgc tttgggggcg cgggtctggt cgagatcctc gggcgtatga
     2881 ccgaggc
//
LOCUS       DATLKL010000599         5121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_6996, whole genome shotgun sequence.
ACCESSION   DATLKL010000599 DATLKL010000000
VERSION     DATLKL010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6996"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VM713_08020"
     CDS             complement(<1..704)
                     /locus_tag="VM713_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HVH84246.1"
                     /translation="MAADAQQSTVMAQVRQSERASGRSLLLLGVLAAGVFCVSLLVGS
                     SGSGVRGALAALSGSGDEATRTVLLAVRLPRVLAAFGVGSLLALAGVLLQALFRNPLA
                     DPYVLGVSGGAAVGALLAMMAGLAALAVQSAAIGGALTAVVTVYLLARGGGTPRLLLT
                     GVVLASACGALVSVLLALADTSRVRGMVFWLAGDLEWAVTPWASAAAAVLAVVAAVLV
                     ARPLNVLAAGELRARSV"
     gene            772..1335
                     /locus_tag="VM713_08025"
     CDS             772..1335
                     /locus_tag="VM713_08025"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HVH84247.1"
                     /translation="MSDVIDPDGFRANVGIVLMHGPNVFLGRRSGGRGWQFPQGGMRE
                     GETPEEAAYRELAEEIGVERDSVELLGSTSDWLRYRLPPRYIRRKQHPLCVGQRQRWF
                     LLRLATEEVRFDFARTAEPEFDRWRWAGWWEPVREVIFFKRSVYARALTELAALAFPA
                     GQSPPLPDWWDKVTAAGGRPPAASASR"
     gene            1336..2097
                     /locus_tag="VM713_08030"
     CDS             1336..2097
                     /locus_tag="VM713_08030"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HVH84248.1"
                     /translation="MPRRRFIAAILGGDDGAPQVVHRRSRARRILVGFATTLIGLFGV
                     YVVYELGRYDAGYDRQAVGQPRTELEVKIEHLEAENRGLRTRLAELDTIRLSRAREQA
                     EVARAMGDLQAQVAKQSQELAFYRGVVAQGAATLGVKIEDLRITAAPQPNVYKAHMAL
                     VRSGRAEALATGTLLLSVDGELAGGEKTLDLATLTGGRQQTLRYDFRYFQDFDQELTL
                     PAGFKAAHLVVEVRSNRGDVTPLSQTFLWSVEASP"
     gene            2122..2517
                     /locus_tag="VM713_08035"
     CDS             2122..2517
                     /locus_tag="VM713_08035"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HVH84249.1"
                     /translation="MFSRDRPPQIDTLIGKGVRVAGDVDFLGGMHLDGAIAGNVRSNP
                     APDSTLSVSETGSVEGSVDVPNVILEGQVRGDIRAAERVVLGASARVEGDVYYGVIEM
                     TVGAQITGKLVRIAPPAAAQAPAQAAAKG"
     gene            2633..2989
                     /gene="erpA"
                     /locus_tag="VM713_08040"
     CDS             2633..2989
                     /gene="erpA"
                     /locus_tag="VM713_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005244817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HVH84250.1"
                     /translation="MTTTDTLQDAPALLFTEAAARKVGDLIRGEGNPKLMLRVFVQGG
                     GCSGLQYGFEFDEQLQEGDTCIENLGVKLIVDPMSVQYLTGSEIDYREGLDGAQFVIR
                     NPNATTTCGCGSSFAA"
     gene            2993..4558
                     /gene="ppx"
                     /locus_tag="VM713_08045"
     CDS             2993..4558
                     /gene="ppx"
                     /locus_tag="VM713_08045"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR03706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HVH84251.1"
                     /translation="MRVPAALSARLAEAKSAVRAVLRPVPDVLAAVDLGSNSFHMVVA
                     RYSHGQLTIIDRLREMVRLAAGVEESGRIDKDVAARALACLERFGQRLRDMHADSVRV
                     VGTNALRMAHRKQAFLERAREALGHPIEIIAGMEEARLIYSGVAHTMPSEAGRRLVVD
                     IGGGSTELIIGEELTPLELESLQMGCVSLSERFFRDGKISAKRFERARLAARLELEPV
                     QAEFRRLGWDNCAGSSGTVRAIGEVIRALEPTSLSITPAGLARAIEYCVDAGHTRELT
                     LAPITEDRQPVFPGGLAILAEVFSVLDIKDMKIAEGAMREGLLYDMLGRFRREDARER
                     TVRAMQQRYHVDASQAERVEATVCDFLEQTRVSWRLEEPLAHLALKWAARLHEIGLDV
                     SHSGYHRHGAYLLENADMPGFPREEQRLLARLVGAHRRKLALDGVEELVPPWDRSAVY
                     LILLLRLAVLLHRGRSATALPPIELSVTPRSIEARFPARWLKDRPLTSADLMQEVDYL
                     RAGGYRLRVFGGS"
     gene            complement(4565..>5121)
                     /locus_tag="VM713_08050"
     CDS             complement(4565..>5121)
                     /locus_tag="VM713_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84252.1"
                     /translation="SPFGMHDMVLEKLAREAEHARAGRPARVIAKMNALVDPESIEAL
                     YRASCAGVKIDLIVRGVCALRPGVPGVSENITVHSIVGRFLEHSRVFYYENAGQPEMF
                     CASADWMERNFFRRVEVAFPIRHQAQEERIQRDLQVCLNDNCQAWKLQPDGSYERIRR
                     GEAKAVNAQMELLAAYAAGPGAAV"
BASE COUNT          784 a         1821 c         1780 g          736 t
ORIGIN      
        1 acgctgcggg cacgcagctc cccggccgcg agcacgttca acggacgcgc gaccagcacc
       61 gcggccacca cggcgagcac cgcggccgcc gcgctcgccc agggcgtcac ggcccactcg
      121 agatccccgg cgagccagaa caccatgccg cgtacacggg aagtgtcggc gagcgcgagc
      181 agtacgctca ccagtgcgcc gcaggcgctc gccaacacca caccggtaag aagcaggcgc
      241 ggcgtgccgc cgccgcgcgc gaggagatac accgtgacca ccgcggtgag cgccccgccg
      301 atcgcggccg actgcaccgc gagcgccgcc aggcctgcca tcatcgcgag cagcgccccg
      361 accgcggctc cgcccgatac gccgaggacg tacggatccg ccagcgggtt gcggaacagc
      421 gcctgcagca gcacgcccgc gagcgccagc agactcccga ccccgaaggc cgccagcacg
      481 cgcggcaagc gtacggcgag cagcacggtg cgcgtcgcct catcacccga gccgctgagc
      541 gccgcgagcg cgccgcgcac gccgctgccc gagctgccga cgagcaacga cacgcagaac
      601 acgccagccg ccaacacacc gagcagcagg aggctgcggc ccgacgcccg ttcggactgg
      661 cgaacctgcg ccatcaccgt cgactgctgc gcgtctgcgg ccataggggc gctagactac
      721 atcctccaat gctcacggcg ggcgcgcctt gtggcagact cgccctcaat catgagcgac
      781 gtcatcgatc cggacggttt tcgcgccaac gtcgggatcg ttctgatgca tgggccgaac
      841 gtgttcctcg ggcgccgcag cggcgggcgc ggctggcagt ttccgcaggg cggcatgcgc
      901 gagggcgaga cgcccgagga ggccgcctac cgcgagctcg ccgaggagat cggcgtcgag
      961 cgcgacagcg tcgagctcct cggctcgacc tcggattggc tgcgctaccg gctgccgccg
     1021 cgctacattc gccgcaagca gcatccgctg tgcgtcggcc agcggcagcg ctggtttctg
     1081 ctgcggctcg ccacggagga ggtaaggttc gatttcgcgc gtaccgccga gcccgagttc
     1141 gatcgctggc ggtgggctgg gtggtgggag ccggtgcgcg aggtgatctt cttcaagcgc
     1201 tcggtgtacg cgcgcgcgct caccgagctc gccgcgctcg cgttcccggc gggccagagt
     1261 ccgccgctgc ccgactggtg ggacaaggtc accgccgcgg gcggccggcc acccgccgcg
     1321 tcggcctcgc ggtaaatgcc ccggcggcgc ttcattgctg cgatcctcgg cggcgacgac
     1381 ggcgcgccgc aggtagtgca ccgccggtcg cgcgcgcgcc gcatactcgt cggtttcgcg
     1441 acgacgctga tcggtctgtt cggcgtgtac gtggtctacg agctcggccg ctacgacgcc
     1501 ggctacgacc gccaggcggt cgggcagccg cgcacggagc tcgaagtgaa gatcgagcac
     1561 ctcgaggccg agaaccgcgg gctgcgcacc cggctcgcgg agctcgacac gatccgcctg
     1621 agccgcgcgc gcgagcaggc ggaggtggca cgggcgatgg gggacctcca ggcgcaggtc
     1681 gccaagcagt cgcaggagct cgccttctac cggggcgtgg tggcgcaggg ggcggcgaca
     1741 ctcggggtga agatcgagga cctgcgcatc acggcggcgc cgcagccgaa tgtctacaag
     1801 gcgcacatgg cgctggtgcg ctccgggcgc gccgaggctc tcgccaccgg gacgctgctc
     1861 ctcagcgtcg acggcgagct cgccggcggc gagaagacgc tcgacctcgc caccctcacc
     1921 ggcggccgcc agcagaccct gcgctacgac tttcgctatt tccaggactt cgaccaggag
     1981 ctgaccctgc cggcgggctt caaggccgcg cacctggtgg tcgaggtgcg ttcgaaccgc
     2041 ggggatgtga cgccgctctc gcagacgttc ctgtggagcg tcgaggcctc gccgtagaat
     2101 acccgtctac cggaggcagc catgttcagt cgcgaccgcc cgccccagat cgataccctg
     2161 attggcaagg gcgtgcgcgt cgcgggcgac gtcgacttcc tcggcggcat gcatctcgat
     2221 ggcgcgatcg ccggcaacgt gcgctcgaac ccggcgcccg actcgacgct ttcggtgagc
     2281 gagaccggct ccgtcgaggg gtcggtcgac gtgcccaacg tgatcctgga gggccaggtg
     2341 cgcggcgaca tccgcgccgc cgagcgcgtg gtgctcgggg ccagtgcgcg cgtcgagggc
     2401 gacgtctact acggcgtcat cgagatgacg gtcggcgcgc agattaccgg caagctggtg
     2461 cgcatcgcgc ccccggccgc ggcgcaggct cccgcgcagg ccgcggccaa gggttgagaa
     2521 gcgagccgca aggcccaaat gagggggtca ggcgcggccc ggacgagaca gcgtgaagtg
     2581 cgcctttgac ccgtcctccg cgggcttcta gactgctcct tagcaggaga ttatgaccac
     2641 caccgacact cttcaggacg ctcccgcgct cctcttcacc gaagccgcgg cccgcaaggt
     2701 cggcgacctc attcgcggcg aaggcaaccc caagctcatg ctgcgcgtgt tcgtgcaggg
     2761 tgggggctgc tcgggactgc agtacggctt cgagttcgac gagcagctgc aggaaggcga
     2821 cacctgcatc gagaacctcg gggtgaagct catcgtcgat ccgatgagcg tgcagtacct
     2881 caccggttct gaaatcgact accgcgaagg gctcgacggc gcacagttcg tcatccgcaa
     2941 cccgaatgcg accactacct gcggttgcgg gtcctccttc gcggcctgac gcgtgcgcgt
     3001 ccccgctgcc ttaagcgccc gcctcgccga agccaaatcc gccgtccgcg ccgtgctgcg
     3061 tccggtcccg gacgtcctgg ccgcggtcga cttgggcagc aacagttttc acatggtcgt
     3121 ggcccgctac tcgcacggcc agctcaccat catcgatcgc ctgcgcgaga tggtgcgcct
     3181 cgccgccgga gtcgaggaga gcgggcggat cgacaaggac gtagcggcgc gcgcgctcgc
     3241 gtgcctcgag cgcttcgggc agcggctgcg cgacatgcac gccgacagcg tgcgcgtcgt
     3301 cggcaccaac gcgctgcgca tggcgcaccg caagcaggcg ttcctcgagc gcgcgcgtga
     3361 ggcgctcggc cacccgatcg agatcatcgc cggcatggag gaggcgcgac tcatctactc
     3421 gggggtcgct cacaccatgc cgagcgaggc cggtcgccgt ctcgtggtcg acatcggcgg
     3481 cggcagcacc gagctcatca tcggcgagga gctgactccg ctcgagctcg agagcctgca
     3541 gatgggctgt gtcagcttga gcgagcgctt cttccgcgac ggcaagatct ccgccaagcg
     3601 cttcgagcgg gcacggctcg cggcacgcct cgagctcgag ccggtgcagg cggaattccg
     3661 ccgcctcggc tgggacaact gcgcgggcag ctccggcacg gtgcgcgcca tcggcgaggt
     3721 gatccgcgcg ctcgagccca cctcgttgtc gatcacgccg gcgggcctgg cgcgcgcgat
     3781 cgagtattgc gtcgacgccg gccacacgcg cgagctgacg ctcgcgccca tcaccgaaga
     3841 ccgccagccg gtgtttcccg gagggctggc gatcctcgcc gaagtgttca gcgtgctcga
     3901 catcaaggac atgaagatcg ccgaaggggc gatgcgcgag ggactgctct acgacatgct
     3961 cggccgcttc cgccgcgagg acgcgcgcga gcgcaccgtg cgcgcgatgc agcagcgcta
     4021 tcacgtcgat gcgagccaag ccgagcgggt cgaggccacg gtgtgcgact tcctcgagca
     4081 gacacgcgtg agctggaggc tcgaggagcc gctcgcgcac ctggcgctca agtgggccgc
     4141 gcgactgcat gagatcgggc tcgacgtttc gcactcgggc tatcaccgcc acggcgccta
     4201 cctgctcgag aacgccgata tgccgggctt cccgcgcgag gagcagcggc tgctggcacg
     4261 cctggtcggc gcgcaccgcc gtaaactcgc gctcgatggc gtcgaggagc tcgttccgcc
     4321 gtgggaccgc agcgccgtct acctcatcct gctgttgcgc ctggcggtgc tgctgcaccg
     4381 ggggcgcagc gccaccgctc tgccgccgat cgagctctcg gtcacgcccc gctctatcga
     4441 ggcgcgcttc ccggcgcgct ggctcaagga ccgtccgctc acctccgcgg atcttatgca
     4501 ggaggtcgat tacctgcgcg ccggcggcta ccggttgcgg gtgttcggcg ggagctgagg
     4561 tgctctagac cgccgcgccg ggtcccgccg catacgcggc gagcagctcc atctgtgcat
     4621 tgaccgcctt cgcttccccg cgccggatgc gctcgtagct gccgtcgggc tgcagcttcc
     4681 aggcctggca gttgtcgttc aggcagactt ggaggtcacg ctggatgcgc tcctcctgcg
     4741 cctggtggcg gatcgggaag gcgacctcca cgcggcggaa gaagttgcgc tccatccagt
     4801 cggcgctcgc gcagaacatt tcgggctggc cggcgttctc gtaatagaac acgcgcgagt
     4861 gctcgaggaa gcgcccgaca atcgagtgca cggtgatgtt ctcggagaca ccggggaccc
     4921 cgggacgcag cgcgcacacg ccgcgcacga tgagatcgat cttcaccccc gcgcaggagg
     4981 cgcggtacag cgcttcgatc gactcgggat cgacgagcgc gttcatcttg gcgatgaccc
     5041 gtgcgggacg tccggcgcgc gcatgctcgg cctcgcgcgc cagcttttcc agcaccatgt
     5101 cgtgcatgcc gaagggtgac t
//
LOCUS       DATLKL010000600         1979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_120098, whole genome shotgun sequence.
ACCESSION   DATLKL010000600 DATLKL010000000
VERSION     DATLKL010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120098"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1747
                     /locus_tag="VM713_08055"
     CDS             <1..1747
                     /locus_tag="VM713_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH84253.1"
                     /translation="DSPYWKTRTKAANPRVVSELKANLRDVATYFHANAQKSGHTSDY
                     EEAARWYRSYLKSFPGDADSAATNYLLAEALFASHHYEEAASEYAHTAYDYPKGDKSA
                     TAAHAGLVAYEKGEASLSGAEKDAWHKRATDAGVKFAESFPEHPDSAGVLTRAAEDIF
                     AAGDRPRAISVAQLLLARQPAVDSAKQRIAWTIIAQSHFDQGEYAQAEPAYVQARALT
                     GGDDKMRSDLTERLAASVYKQGEAKQKAGDGTGAVEDFLRVARVAPDSKIRSNAEYDA
                     AAQLIQLQSWDRAIGVLEGFRRNFPASPLAADVTRKLAVAYGAANRPGDAAAEFERIA
                     ASPTEQAAVRREAQMQAADLYAKSGNTPKAVAMLEKFVAANPTPVADAVEARQRLADY
                     AARAGDSAQRDRWYREIIAADAQAGAQRTERTHYLAAKAQLTLAAPARDAFRAVRLSA
                     PLKKSLVVKRHALEAAMDGYKTAADYQVAEVTTAATYEMAELYRTLAHDVMNSERPSN
                     LKGDALEEYNSLLEEQVFPFEEQAIKAHELNAARAKDGLYDEWVRKSFGALAELKPAR
                     YGKTELTEDVVAKLE"
BASE COUNT          346 a          711 c          678 g          244 t
ORIGIN      
        1 cgattcgccc tactggaaga cccgcaccaa ggcggccaac cccagggtgg tgtccgagct
       61 caaggccaac ctgagggacg tcgccacgta tttccacgcc aacgcgcaga agagcggcca
      121 cacctcggat tacgaggagg cggcgcgctg gtatcgctcg tacctcaagt ccttcccagg
      181 cgatgcggac tcggcggcga ccaactacct gctggccgag gcgctgttcg cgagtcatca
      241 ctacgaggag gcggccagcg aatacgccca cacagcctac gactatccga agggcgacaa
      301 gtcggccacc gcggcccacg ccggcctggt cgcgtatgaa aagggcgagg cgagtctctc
      361 cggcgccgag aaggacgcct ggcacaagcg cgccaccgat gccggggtga agttcgctga
      421 gtccttcccc gagcatcccg acagcgccgg cgtcctgaca cgcgccgccg aggacatctt
      481 cgcagcgggc gaccggccgc gcgccatcag cgtcgcgcag ctgttgctgg cgcgtcagcc
      541 cgcggtcgac tccgccaagc agcgcatcgc ctggaccatc atcgcgcagt cgcacttcga
      601 ccagggcgag tacgcgcagg ccgagcccgc ctacgtgcag gcgcgggcgc tcaccggcgg
      661 cgacgacaag atgcgctccg atctcaccga gcgtctggcg gcatccgtct acaagcaggg
      721 cgaggcgaaa cagaaggccg gcgacggcac gggcgcggtc gaggactttc tgcgcgtcgc
      781 gcgcgtggcg cccgattcca agatccgctc caacgccgag tacgacgccg ccgcgcaact
      841 gatccagctg cagagctggg accgcgccat cggcgtcctg gaaggcttcc ggcggaactt
      901 cccggcgagc ccgctcgcgg ccgacgtgac gcgcaagctc gccgtcgctt acggcgcagc
      961 caaccggccg ggcgatgcgg cggcagaatt cgagcgcatc gccgcgagtc cgaccgagca
     1021 ggcggccgtg cggcgcgagg cgcaaatgca agccgcggat ctctacgcca agtccggcaa
     1081 caccccgaaa gcggtggcca tgctcgagaa gttcgtcgcc gccaacccga cgccggtcgc
     1141 ggacgccgtc gaggcacgcc agcgcctcgc cgactacgcc gcacgggcgg gcgatagcgc
     1201 gcagcgcgat cgctggtacc gggagatcat cgcggccgac gctcaggcgg gggcgcagcg
     1261 caccgagcgc acgcactatc tggcggccaa ggcgcaactc acgctcgccg ccccggcgcg
     1321 tgatgccttc cgcgcggtgc gcctcagcgc gccactgaag aagagcctcg tggtcaagcg
     1381 ccacgcactc gaggcggcga tggacggcta caagacggcg gccgactacc aggtcgccga
     1441 ggtgactacg gcggcgacct acgagatggc cgagctctac cgcactctgg cccacgacgt
     1501 gatgaattcc gagcgtccgt cgaatctcaa gggcgacgcg ctcgaggaat acaactcgct
     1561 gctcgaggaa caggtgtttc ccttcgagga gcaggcgatc aaggcgcacg agctcaacgc
     1621 cgcgcgtgcc aaggatggcc tctacgatga atgggtccgt aagagcttcg gggcactcgc
     1681 tgagctcaag cccgcacgct acggcaagac ggagctgacc gaggatgtgg ttgcaaagct
     1741 cgaataagcg cgccgctgcg cgcctagcga ctctcgcgcg ggtggtgcga ccggcgtgcg
     1801 ccgcgctgtt gttggtcgcg ggctgccgtt cggcgccccc cgagcccgag cgccctgcgg
     1861 cgcgggagcc ggtaccgcgc gcggcgacct ctccggcacc ggcggcgccg accagctccg
     1921 cgacgcggcc gagcgctgcg cccggcgtgg ctggccagcc cgcgggcaac gccagcgcc
//
LOCUS       DATLKL010000601         2424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_63714, whole genome shotgun sequence.
ACCESSION   DATLKL010000601 DATLKL010000000
VERSION     DATLKL010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63714"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /gene="pepQ"
                     /locus_tag="VM713_08060"
     CDS             <1..1204
                     /gene="pepQ"
                     /locus_tag="VM713_08060"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: protein motif:HMM:NF010133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HVH84254.1"
                     /translation="TYPFVAHAPFKVWVPLPDAPDSFIWFEPGSRPKLILNQPRDYWY
                     KPADLPRGYWVRHFDVLPVPDSAAARAALPRDLSRAAYIGDATPEIAAWGPGAVNPRT
                     LMRTLDYDRAVKSPYELTCLRAANRRGARGHLAATRAFEAGASEFEIELEFISACGLR
                     EQELPYNPIIALNEGGSVLHYQVLNKQPPRERHSLLIDAGAEFAGYASDITRTHAYAD
                     GDFRALIARMDELQQGLCAGVRAGVDWREVHLRAHVETGRLLEEADLVRCSGEEAVAT
                     GVTKVFLPHGIGHLLGIEVHDVGGFMKAGGDGDIPRPEGHPYLRLTRTLEPGFVVTME
                     PGIYFIPQLLEAARANDAQASRINWPRVAALTKFGGIRIEDDLAITQSGCENLTRDAF
                     RAAAAGHA"
     gene            complement(1404..1805)
                     /gene="mscL"
                     /locus_tag="VM713_08065"
     CDS             complement(1404..1805)
                     /gene="mscL"
                     /locus_tag="VM713_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015379263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HVH84255.1"
                     /translation="MSFASEFKEFAMKGSVIDLAVGVVIGGAFGKIVSSLVGDVIMPA
                     LSPILGSVNFSDLSFQIATNAAGKPVLLRYGAFIQTIFEFLVIALSIFIVIKGINKLK
                     RPPPTAPDAPPPAPTKTEALLQDIKDLLAKR"
     gene            complement(2062..2418)
                     /gene="rplQ"
                     /locus_tag="VM713_08070"
                     /pseudo
     CDS             complement(2062..2418)
                     /gene="rplQ"
                     /locus_tag="VM713_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
BASE COUNT          392 a          814 c          799 g          419 t
ORIGIN      
        1 aacgtacccc ttcgtggcgc acgccccctt caaggtgtgg gttccactcc cggatgcgcc
       61 cgactcgttc atctggttcg agcccgggtc gcggccgaag ctgatcctca accagccgcg
      121 cgactactgg tacaagcccg cggacctgcc gcgtggctac tgggtgcgtc atttcgacgt
      181 cctgcccgtc ccggacagtg ccgccgcgcg cgccgcgttg ccccgtgacc tgtcacgggc
      241 ggcctacatc ggggatgcga cgcccgagat tgccgcctgg gggccggggg cagtcaatcc
      301 acgcacgctc atgcgcacgc tcgattacga ccgcgccgtc aagagcccct acgagctcac
      361 ctgtctgcgg gccgcgaacc ggcggggcgc ccgtggacat ctcgcggcca cgcgtgcctt
      421 cgaggccgga gcgtccgagt tcgagatcga gctcgagttc atctccgcct gcgggctgcg
      481 cgagcaggag ctgccgtaca acccgatcat cgcgctgaac gagggcgggt cggtgctgca
      541 ctaccaggtg ctcaacaagc agccgccgcg ggagcgtcat tcgctcctga ttgacgccgg
      601 cgcggagttc gccggctatg cgagcgacat cacccgcacc catgcgtacg cggatggaga
      661 ctttcgcgcg ctcatcgcgc ggatggacga gctgcagcaa gggctgtgcg ccggcgtacg
      721 cgccggcgtc gactggcgcg aggtacacct cagggcacac gtcgagacgg gccggctgct
      781 cgaggaggcg gatctggtgc gctgcagtgg cgaggaggcg gtcgccacgg gggtcacgaa
      841 ggtgttcctg ccgcacggca tcgggcatct gctcgggatc gaggtccacg acgtcggcgg
      901 attcatgaag gccggcggcg acggcgacat cccccggccc gagggtcacc cgtacctgag
      961 gctcacacgc acgctcgagc ccggcttcgt cgtcaccatg gagccgggaa tctatttcat
     1021 tccgcaactg ctcgaggcgg cccgggctaa cgacgcccaa gcgagccgca tcaattggcc
     1081 gcgggtcgcg gcgctcacga aattcggcgg catccgcatc gaggacgatc tggcgatcac
     1141 gcaatccggg tgcgagaacc tgacgcgcga cgccttccgc gccgccgcgg cagggcacgc
     1201 gtgaacaccg gctgagcgta tgtgataccc cgaaaatgac tccgccccgc aataggtaga
     1261 aaagcgtcta tctatggctc cttgaggccc gctacagggg tcccagagag catacataga
     1321 tgactttcga cctattgcgc gtcgaaagca ttttcgggca tcaccacacg cacactcgcg
     1381 atcgccgact gcgctcctac cagctaacgc ttggccagca gatcctttat gtcctgcagc
     1441 aatgcttcgg tcttcgttgg tgccggcggg ggtgcatcgg gcgccgtcgg cggcgggcgc
     1501 ttcagcttat tgatgccctt gatgacgatg aagatcgaga gcgcgatcac caggaactcg
     1561 aagatcgtct gaatgaatgc cccgtagcgc agcagcacgg gtttgcccgc cgcattggtg
     1621 gcgatctgga acgacaggtc gctgaagttc acgctcccga ggatgggcga cagcgccggc
     1681 atgatgacgt cgccgaccag cgaggagacg atttttccga aggctccgcc gatcacgaca
     1741 ccaaccgcga ggtcgatgac gctgcctttc atcgcaaatt ctttgaattc agaagcgaaa
     1801 ctcatggcgg gccctcctcc cctcgagcac cggtcgtagt tcaggggcgc gcaacggcgc
     1861 ccgcaggaat caggcgggcg ccttcttccg gcgggtccgc ttcttcggct cctcggccgg
     1921 ctcggcagct ggctttgcct tcttcttggg ctccgccttc gcctcggctt tgggcgcggc
     1981 cttttcctcg gccttggctt tcgcgcgtag gcgagctttc ttcgcctctg cggcgggcgg
     2041 ttgctcggcg gcagcaggcg cggggccctc gaccagctgc atgagggcca tcggcgccga
     2101 atcacccggc cgcggcacca tgcgcaggat ccgcgtgtat cccccagggc gcgctttgaa
     2161 gcggggcccc aggtccgcga acagcttctc caccacttcg gcgtcgcgca gccgggcgaa
     2221 ggccaggcgg cggttcgcct ctgcgtcgct cttggcgagc gtgatgagcg gctcgaccac
     2281 gcggcgcagt tccttcgcct tcggcagcgt ggtgcggatc gtctcgtgcc gcagcagcga
     2341 gaccgacata ttgcgcagca gcgcgtgccg gtgcgtgctg ttacgcgaca gctgccggcc
     2401 ggagagatgg tgacgcataa ataa
//
LOCUS       DATLKL010000602         1596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_131540, whole genome shotgun sequence.
ACCESSION   DATLKL010000602 DATLKL010000000
VERSION     DATLKL010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131540"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1596
                     /gene="acnA"
                     /locus_tag="VM713_08075"
     CDS             <1..>1596
                     /gene="acnA"
                     /locus_tag="VM713_08075"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HVH84256.1"
                     /translation="FSVVPPNTGIVHQVNLEYLGRVIFDAESGGARRAYPDTLVGTDS
                     HTTMINGLGVLGWGVGGIEAEAAMLGQPVTMLIPQVIGFRLEGRLQPGATATDLVLTV
                     TEMLRKKGVVDKFVEFFGDGLANLPLADRATIANMAPEYGSTCGIFPIDAETIRYLEL
                     SGRPRERIELVGTYARAQGMWRNPGATPARYTDELELDLGKVEPSLAGPKRPQDRVPL
                     KSAKKVYETNAKKAAEERAARNGASGVAPAKLNGQTVELKDGAVLIAAITSCTNTSNP
                     SVMLGAGLLARKARERGLTAKPWVKTSLAPGSRVVTDYFKKAGVLDDLAAVGFDLVGY
                     GCTTCIGNSGPLKPEISAAVKQGDLTACSVLSGNRNFEGRVHPEVRMNFLASPPLVVA
                     YALAGSLDVDLTTEPLGTDRDGKPVHLADIWPTDAEVQEVLKRSIDSQMFRHSYASVF
                     QGDENWAGIQVPAGKRYAWDEKSTYVKNPPYFDGMTMTPPPLADVRGARALAVLGDSV
                     TTDHISPAGNISRSSPAARYLIDQ"
BASE COUNT          274 a          568 c          513 g          241 t
ORIGIN      
        1 ttctcggtgg tgccgcccaa caccggcatc gtccaccagg tgaatctcga gtacctcggg
       61 cgcgtcatct tcgacgccga gtcgggtggc gcgcgtcgcg cctaccccga caccctggtc
      121 ggcaccgact cgcacaccac catgatcaac ggactgggcg tcctcgggtg gggcgtcggt
      181 ggcatcgagg cggaagcggc catgctcgga caaccggtga ccatgctcat cccgcaggtc
      241 atcggttttc gactcgaggg ccggctgcag cccggcgcca cggcgacgga cctggtgctg
      301 acggtcacgg agatgctgcg caagaagggc gtggtggaca agttcgtcga gttcttcggc
      361 gacggcctgg cgaatcttcc gctcgccgac cgggcaacca tcgccaacat ggcgcccgag
      421 tacggcagca cctgcggaat cttcccgata gacgccgaga cgatccgtta tctcgagctc
      481 tccggccggc cgcgcgagcg catcgagctg gtcggcacct atgcgcgcgc ccagggcatg
      541 tggcgcaatc cgggcgcgac tccggcccgc tacaccgacg agctcgagct cgacttgggg
      601 aaagtggagc cgagcctcgc cgggcccaag cgtccgcagg accgcgtgcc cttgaagagc
      661 gccaagaagg tctacgagac caacgcgaag aaggccgcgg aagagcgcgc ggcgcgcaac
      721 ggcgcgagcg gtgtcgcgcc ggccaagctc aacggccaga cggtggagct gaaggatggc
      781 gcggtgctga tcgcagcgat caccagctgc accaacacct cgaatccctc cgtcatgctc
      841 ggcgccgggc tcctcgcccg caaggcgcgc gagcgcggct tgaccgccaa accctgggtg
      901 aaaaccagcc tcgcgcccgg ctcgcgcgtc gtgaccgact acttcaagaa ggccggcgtg
      961 ctcgatgatc tcgcggcggt ggggtttgat ctggtgggct atggttgcac cacgtgcatc
     1021 ggaaattccg gccccctcaa gcccgagatc tccgccgccg tcaaacaagg cgacctcacc
     1081 gcctgctccg tgctctccgg taaccgcaac ttcgaggggc gcgtgcatcc cgaagtgcgc
     1141 atgaactttc tcgcttcacc gccactcgtt gtcgcctacg cgctcgccgg gtcgctcgac
     1201 gtcgatctca ccaccgagcc gctcggcacc gaccgcgacg gcaagcccgt gcacctcgcc
     1261 gacatctggc cgaccgatgc cgaggtccag gaggtcttga agcgctcgat cgactcgcag
     1321 atgttccgcc acagctatgc gagcgtgttc cagggcgacg agaactgggc cggcattcag
     1381 gtgcccgccg gcaagcgcta cgcctgggac gagaaatcga cctacgtgaa gaatccgccc
     1441 tacttcgacg ggatgaccat gacgccgccg ccgctcgccg acgtgcgcgg tgcgcgcgcg
     1501 ctcgccgtgc tcggcgactc ggtgaccacc gatcacatct cgccggccgg caacatctcg
     1561 cgctcgagcc cggcggctcg ctacctcatc gaccag
//
LOCUS       DATLKL010000603         1823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_41200, whole genome shotgun sequence.
ACCESSION   DATLKL010000603 DATLKL010000000
VERSION     DATLKL010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41200"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..998)
                     /locus_tag="VM713_08080"
     CDS             complement(222..998)
                     /locus_tag="VM713_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVH84257.1"
                     /translation="MATTVGDGGHSGGSMRIQFLGSGDAFGSGGRFNTCILVEAGTAV
                     FLIDCGASSLIAMRRFRVTPNRVQTIFITHLHGDHFGGLPFFILDAQLVSRRIEPLTI
                     AGPPGLRKRLAEASEALFPGSSRVERRFAIETIELQPGASRSINGVLVTPYLVAHPSG
                     APPLALRLEAEGKVVAYSGDTEWTDALVPAARGADLFISEAYYFEKQVKFHLDYQTLQ
                     RHLGELQPKRLMLTHMSEDMLARLGALEHETAEDGMAIEL"
     gene            1067..1447
                     /locus_tag="VM713_08085"
     CDS             1067..1447
                     /locus_tag="VM713_08085"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HVH84258.1"
                     /translation="MMRATLAFAGLSGVVAIALGAWMAHGATAVVDPHSLEWVRTGVQ
                     YQAWHTVALVGVAVLMAVRPGRFLGAAATAFAVGVLLFSGSLYGLALTGARTFAYITP
                     VGGVALIAGWLLLAIYGLSLDRRG"
BASE COUNT          308 a          589 c          601 g          325 t
ORIGIN      
        1 gggcctaacc ggcaaaaaac gcttcggtgg tggtgaccga actacctgga aacctttttt
       61 tgaggctcac tggcccgccc agtgccgagg aggcccgcgc tctcgaccgg cagggtcaac
      121 gcatcgccga cctcgaagtc gtcatcctcg catgaggccg gagccctggc ctaccctact
      181 acgccatagc gccgtgaggc cgtggtgccg gagcgtcctg ctcagagctc gatcgccatg
      241 ccgtcctcgg cggtctcgtg ctcgagtgcg ccgagtcgcg cgagcatgtc ctcgctcatg
      301 tgcgtaagca tcagccgctt cggctgaagc tcgccgagat gacgctggag cgtctgataa
      361 tccagatgga acttgacctg cttctcgaaa tagtaggcct cgctgatgaa gagatccgcg
      421 ccgcgcgcag ccggcaccag cgcgtcggtc cactccgtat ccccggaata ggccacgact
      481 ttcccctcag cctcgagacg aagggccaag ggcggcgcac cgctcggatg ggcgacaagg
      541 tagggtgtca ccaagacgcc gttgatggac cgggacgccc cgggctgaag ctcgatggtc
      601 tcgatcgcaa atcgccgctc gacccgggat gagcccggga aaagcgcctc tgaggcctcg
      661 gcgagccgct tgcggaggcc gggcgggccg gcgattgtca gcggctcgat gcgccggctg
      721 accagctgcg cgtccagaat aaagaacggc agcccgccga agtggtcgcc gtgcaagtga
      781 gtgataaaaa tggtctggac ccgatttggc gtgacccgga accgccgcat tgcgatcaag
      841 gacgaagcgc cgcagtcgat gaggaagacg gccgttcccg cctcgaccag gatgcaggtg
      901 ttgaaacggc cgccgctgcc gaaggcgtcg ccgctgccaa ggaactggat tcgcatcgag
      961 cctccgctat ggccaccatc accgaccgtc gtcgccacat gcaacgactg accagcgctc
     1021 gagttggcat cacggtcccg caggtgtcgt caagtgctaa gctaccatga tgcgcgctac
     1081 ccttgccttt gccggcctca gcggagtcgt ggcgatcgcg cttggcgcat ggatggccca
     1141 tggcgcgacc gcggtcgtcg acccgcactc gctcgagtgg gtccgtaccg gcgtgcagta
     1201 ccaggcctgg cacaccgtcg ccctggtcgg cgtcgcggtg ctcatggcag tgcggccggg
     1261 gcgctttctc ggcgcggcag cgacggcttt cgcggttggc gtgctgctgt tctccgggag
     1321 cctctacggc ttggcgctga ccggagcccg gactttcgcg tatattacgc cggtcggcgg
     1381 agttgccctg atcgcaggct ggctactgct cgcgatttac gggctctcgc tcgaccgccg
     1441 cggttgatcg aaacgcggcg gacgattgcg cttacgccgg ctactcgctc aagcggcgcg
     1501 gcaaaccacg cgggcgcgcg cgaacgtcgc acacggagcg cagatccacg cgctcgcgtg
     1561 tcccacggcc gagaaatacc ttcaccgcga tctgagtgcc gctataagat gaggatcggt
     1621 ttggccaatg tccggcgatt tggcggccgc gggcaagcgt gcctggccat gcgagccgaa
     1681 ggaattggat tgtgagcgtc gtcttggagc attttgtcga gcaggcctcg gatgttcgcg
     1741 acgcgcgcac gcttgccggg gtgatcgagc gcgccgccaa cgagctagat tttccctatt
     1801 ttgcctattt cgcgctcgat cgg
//
LOCUS       DATLKL010000604         1518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_75205, whole genome shotgun sequence.
ACCESSION   DATLKL010000604 DATLKL010000000
VERSION     DATLKL010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75205"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VM713_08090"
     CDS             <1..782
                     /locus_tag="VM713_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH84259.1"
                     /translation="EELDAAGYARRGSASASRRDYEHAIADLTRAIELDPTQAGFFYE
                     RGTAYGSAGQTDRALEDFGQAIKLKPDNVPALMARAELRVRRHDPADLIGPDLDAANR
                     AAPRQGDMRLALGGLYAHLGNYPAAIGQYDEWIDSHHSDDVRMREARNERCWVRALTG
                     QELDQALRACNAAVRADPKVAAFLDSRGLVYLREGNYDKAVADYDAALALDPKMAWSL
                     YGRGLAKQHLGQAAAGQADIAAATALAPKIAAEAASHGVVP"
     gene            977..>1518
                     /locus_tag="VM713_08095"
     CDS             977..>1518
                     /locus_tag="VM713_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HVH84260.1"
                     /translation="MTPRTAGSIAALALLLSPLAAHAQDASTFPFLEATVAQLEAQMA
                     AGTLTSEQLTRAYIERIEQLDTGGPGVGVNALIELNPDALAIARHADALRRHGKVLGP
                     LHGIPVLLKANIDTGDKMQTTAGSLALQGTPAQRDSTVAANLRAGGMVILGKTNLSEW
                     ANFRSFFSVSGWSAVGGQTH"
BASE COUNT          258 a          539 c          518 g          203 t
ORIGIN      
        1 gggaggagct cgacgcggcg ggctacgcgc ggcgcgggag tgcctcggcg tcgcgccgcg
       61 attacgaaca cgccatcgcc gacctcacga gggcaatcga gctcgacccg actcaggctg
      121 gctttttcta tgagcgtggc accgcgtacg ggtcggcagg ccagacagac agggcgctcg
      181 aggacttcgg tcaggcgatc aagctcaagc ccgacaacgt gccggcgctc atggcccgcg
      241 cggagctgcg cgtgcggcgc catgatcccg cggacctcat cggtcccgac cttgacgccg
      301 ccaaccgtgc cgcgccccgg cagggggaca tgcgcctggc gctgggcggt ctctacgcac
      361 acctcggcaa ctatccggcc gccatcggtc agtacgacga gtggatcgac tcccatcaca
      421 gcgacgacgt gcggatgcgg gaggcgcgca acgagcgctg ctgggtccgg gcgctgacgg
      481 gccaggagct cgaccaggcg cttcgcgctt gcaacgccgc ggtgcgcgcc gacccgaagg
      541 tggcggcgtt tcttgacagc cgtggactcg tctacctgcg cgagggcaac tacgacaagg
      601 cggtcgccga ctacgacgca gcgctcgcgc tcgaccccaa gatggcctgg tcgctgtacg
      661 gccggggact cgcgaagcag cacctggggc aggccgcggc cggccaggcc gatatcgccg
      721 cggcaaccgc gctcgcgccg aagattgccg cggaagctgc gagccacggc gtcgtgccat
      781 aactacacgg cccgcgggcc gcacgggaac gccgttcgcg ctcgtctgat agaagttgaa
      841 atatcgcgcg cgtagcattc gccgcctggc gacgcagccc acacgccgcc ataaccaaca
      901 tcgaggccgg tgcagggagg cacgcgatgg tcgccttccc gccggtccag gctcgcgaac
      961 ccagaggagg aagcacatga caccgcgcac cgccggatcg attgccgcgc tggcactttt
     1021 actctcgccg ctcgccgcgc acgcccagga cgccagcacc tttccgtttc tcgaggcgac
     1081 cgtggcccag ctcgaggccc agatggcggc cgggacgctg acgtccgagc agctgacgcg
     1141 cgcctacatc gagcgcatcg agcagctcga caccggcggc ccgggcgtcg gagtcaacgc
     1201 gctgatcgag ctgaacccgg acgcgctcgc catcgcccgt cacgccgatg cgctgcgccg
     1261 ccacggcaag gtgctcgggc cgctgcacgg cattccggtg ctgctcaagg ccaacatcga
     1321 caccggcgac aagatgcaga cgactgcggg ctcgctcgcg ctgcagggaa caccggcgca
     1381 gcgtgactcg accgtcgcgg cgaacctcag ggcaggggga atggtgattc tcggcaagac
     1441 caacctgtcg gagtgggcga acttccgctc cttcttctcg gtgagcggct ggtccgcggt
     1501 cggcggacag acccacaa
//
LOCUS       DATLKL010000605         2950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_165305, whole genome shotgun sequence.
ACCESSION   DATLKL010000605 DATLKL010000000
VERSION     DATLKL010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165305"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VM713_08100"
     CDS             complement(<1..219)
                     /locus_tag="VM713_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84261.1"
                     /translation="MVEPGTATVARATPAARVALALIVLAACALTASTWHVFFATWDE
                     PEHLAAGVELLDRGYYEYDTEHPPIGRVV"
     gene            complement(212..1507)
                     /locus_tag="VM713_08105"
     CDS             complement(212..1507)
                     /locus_tag="VM713_08105"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HVH84262.1"
                     /translation="MGRFVVVIGTQWGDEGKGKVVDLLTERAAAVARFQGGHNAGHTL
                     VIGGRKTVLSLIPSGILRQEVRCFIGNGVVLSLEALFAESDMLIEQGVPVFERLRISP
                     LCPLILPSHVLLDQARERARGVNAIGTTGRGIGPAYEDKVARRAVRVADLFHRDRFAA
                     KLGEVLDFHNFLLQSYYRMPPVDFQQTLDAQLALGARLAPLVTDVTRSLAELRVSGAN
                     VLFEGAQGAMLDVDLGTYPYVTSSNTTAGFAATGTGLGPRAFDAVLGIVKAYTTRVGA
                     GPFPTELFDAYGEHLSRVGHEFGSVTGRRRRCGWFDSAALKRAVLNSSVSGLCITKLD
                     VLDGLDTIRIGVGYRIDGVVTPEPPLTVEGYAGIEPVYEELPGWRESTVGITAYDALP
                     GNARRYLERLEALAQVPLEMISTGPDRDQIIVLRHPFGG"
     gene            complement(1514..1702)
                     /locus_tag="VM713_08110"
     CDS             complement(1514..1702)
                     /locus_tag="VM713_08110"
                     /inference="COORDINATES: protein motif:HMM:NF021366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HVH84263.1"
                     /translation="MNLSDLAAAFGLFLVLEGVAPFINPQAVKRALARLTEVRDRELR
                     LAGLGSMLVGVLILFVAR"
     gene            complement(1713..2591)
                     /locus_tag="VM713_08115"
     CDS             complement(1713..2591)
                     /locus_tag="VM713_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HVH84264.1"
                     /translation="MSGRLFPVLLALAVALVLVGLSLFTVSETEVAIVTEFGAVLGSH
                     YVPGLHAKWPWDVVTKCDRRILSQSYNGETFLTNDNRGLIVDFYIKWRVKEPVQFYTA
                     TGGRVELAGERLADIVKDGIKGVVAQRTLQQIVSAERAAVTSEMFGQASKSGGGLGVE
                     LVDVRVQRIDLPDDVAARVFESMKQSFAKTAGRLRAEGQSTSTGIRATAERRRTEILA
                     NAERDALHIRGEGDAAATQTYARAYSKDPEFYAFYRSLQAYERSLGKDGDLLVLTPDG
                     EFFKYLKDPDKRRGAK"
     gene            complement(2591..>2950)
                     /locus_tag="VM713_08120"
     CDS             complement(2591..>2950)
                     /locus_tag="VM713_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84265.1"
                     /translation="VAQGSAGRMQQDAEAYRAQVTALAEGQASRFTQLEGAYALAPEV
                     TRRRMYVDAVESLLARAHKVLIDARAGSNMIYLPIDKLLEKQTGTAQETGEQAASGPA
                     KEQPEQVTIEARGRGER"
BASE COUNT          465 a         1035 c         1002 g          448 t
ORIGIN      
        1 taccacgcgg ccgatcggcg ggtgctcggt gtcgtattcg tagtagccgc gatcgagcag
       61 ctccactccg gcggcgagat gctccggctc gtcccaggtc gcaaagaaca cgtgccaggt
      121 gctggccgtg agcgcgcagg ccgccagcac gatcagagcg agtgcgacgc gcgcggcggg
      181 agtcgcgcgc gccaccgtcg cggtgcccgg ctcaaccacc gaatggatgc cgcagcacga
      241 tgatctggtc gcggtccggg ccggtggaga tcatttcgag cggcacctgc gcgagcgcct
      301 cgagccgctc gagatagcga cgggcattac cggggagcgc gtcgtaggcg gtgatcccga
      361 cggtcgactc gcgccagccc ggcagctcct catacaccgg ctcgataccc gcgtagccct
      421 ccacggtgag cggcggctcg ggcgttacga cgccatcgat gcgatagccc acccctatgc
      481 ggatggtatc gagcccatcg agcacatcga gcttggtaat gcacaggccc gagacgctcg
      541 agttcagaac cgcgcgtttc agggccgccg aatcgaacca gccgcagcgg cggcggcgtc
      601 cggtcaccga gccgaactcg tgaccgacgc gcgacaggtg ctcgccgtag gcgtcgaaga
      661 gctccgtcgg aaacggcccg gcgccgaccc gggtcgtgta ggccttgacg atgccgagca
      721 cggcgtcgaa cgcgcgcgga cccaggccgg tgcccgtcgc ggcgaagccc gccgtggtgt
      781 tggacgaggt gacgtaagga taggtgccga ggtccacgtc gagcatcgcc ccctgcgcgc
      841 cctcgaacag cacattggcg ccggagaccc gcagctccgc cagcgagcgc gtcacgtcgg
      901 tcacgagtgg cgcgagccgc gcaccgagcg cgagctgtgc atcgagcgtc tgctgaaaat
      961 cgaccggcgg catgcggtag tagctctgca gcaggaagtt gtggaagtcg agcacttcgc
     1021 cgagcttggc ggcgaagcgg tcgcggtgga aaaggtccgc gacccgcaca gcgcggcgcg
     1081 ccaccttgtc ctcgtaggcc ggccctatgc cacgtccggt ggtgccgatg gcgttgacgc
     1141 cgcgggcccg ctcgcgcgcc tgatcgagca gcacgtgcga gggcagaatg agcgggcaaa
     1201 gcggcgagat cctcagccgc tcgaacaccg gcacaccctg ctcgatgagc atgtcgctct
     1261 cggcgaacag cgcctcgagc gacagcacca cgccgttgcc gatgaaacag cgcacctcct
     1321 gccgcaggat ccccgaggga atgagcgaca gcacggtctt gcggccgccg atcaccagcg
     1381 tgtggccggc gttgtgtccg ccctgaaagc gcgccaccgc ggccgcgcgc tcggtgagca
     1441 ggtcgaccac cttgcctttg ccttcatcgc cccactgggt gccgatgacg accacgaatc
     1501 tgcccatgtg tgcctaccgc gccacgaaca ggatgagcac gccgaccagc atgctgccga
     1561 gacccgcgag gcgcaactcg cggtcgcgca cctcggtgag gcgcgcgagc gcgcgcttca
     1621 ccgcctgcgg attgatgaac ggggcgacgc cttccaacac gaggaacagg ccgaacgcgg
     1681 ccgcgaggtc cgagagattc acgccgccgg cttcacttcg ctccgcggcg cttgtccggg
     1741 tccttcaggt acttgaagaa ctccccgtcg ggagtcagta ccaggagatc cccgtccttg
     1801 ccgagggagc gctcgtaggc ctggaggctc cggtagaagg cgtagaactc cgggtccttc
     1861 gagtaggcgc gcgcgtaggt ctgggtggcg gctgcatcgc cctcgccgcg gatgtgcagc
     1921 gcatcgcgct cggcgttggc gagaatctcg gtgcggcggc gctcggccgt cgcgcggatt
     1981 ccggtgctgg tgctctgccc ttcggcgcgc agccgcccgg cggtcttggc gaagctctgc
     2041 ttcatgctct cgaagacgcg cgccgcgacg tcgtccggca gatcgatgcg ctgcacgcgc
     2101 acgtcgacca gctcgacgcc gagcccgccg ccgctcttgc tcgcctgccc gaacatctcg
     2161 ctcgtgaccg cggcccgttc cgcggacacg atctgctgca gcgtgcgctg cgccaccacg
     2221 cccttgatcc catccttgac gatgtcggcg aggcgctcgc cggccagctc aacccgaccg
     2281 ccggttgcgg tgtagaactg caccggctcc ttgacgcgcc acttgatgta gaagtcgacg
     2341 atcagtccgc ggttgtcgtt ggtgaggaag gtctcgccgt tgtaggactg cgagagaatg
     2401 cgccgatcgc acttggtgac cacgtcccag ggccacttgg cgtgcaaccc cggcacgtag
     2461 tggctgccga ggacggcgcc gaactcggtg acgatggcga cttcggtctc gctcaccgta
     2521 aagagcgaca ggccgacgag caccagcgcc accgcgagcg ccaacagcac cgggaacagc
     2581 cgtccgctca tcagcgctcc ccccgtccgc gcgcctcgat ggtcacctgc tcgggctgct
     2641 ccttggcggg gccgctcgcg gcctgctcgc ccgtctcctg ggcggtgccc gtctgcttct
     2701 cgagcagctt gtcgatcggc aggtagatca tgttgctgcc ggcgcgcgcg tcgatgagca
     2761 ccttgtgggc acgcgcgagc aggctctcca ccgcatcgac gtacatgcgc cggcgcgtca
     2821 cctcgggggc gagcgcatag gcgccctcga gctgcgtgaa gcgagacgcc tgaccctcgg
     2881 cgagtgcagt cacctgcgcc cgataggcct cggcgtcctg ctgcatgcgt ccggccgagc
     2941 cctgcgccac
//
LOCUS       DATLKL010000606         1801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_142864, whole genome shotgun sequence.
ACCESSION   DATLKL010000606 DATLKL010000000
VERSION     DATLKL010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142864"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VM713_08125"
     CDS             complement(<1..331)
                     /locus_tag="VM713_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84266.1"
                     /translation="MPSRVPQGERRGWIIAIGGAENKENDRRILERFVSASGGGQADI
                     VVIPTASRVHETGPRYERIFSDLGAARVTVMDFDTRRDCQEPGRLERLSQSYFFFSFL
                     GSATRSDQ"
     gene            complement(502..1731)
                     /locus_tag="VM713_08130"
     CDS             complement(502..1731)
                     /locus_tag="VM713_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HVH84267.1"
                     /translation="MTHVAGVDEIRSHFPALERTYNGFPVAYFDGPGGTQVPRAVAAA
                     MTDYLLNHNANTHWDFPSSHETDLALGAARAALADWLGGSADEIVFGANMTTLTFHLG
                     RTLGRGWRAGDVVVVTELDHHANADTWRALARERGLEIRAIRMRREDGRLDEADAAAK
                     LTPGVRLLALGAASNALGTINDVAQLTARAHAVGAWVFIDGVHYASHQMPDVRAWDCD
                     FFACSAYKFYGPHVGVLWGRRELLAALDPPKLAPAPGEPPELLETGTLNHEGIVGAAA
                     TVDFLASLAGERLPRRERLARLSQTLHERGEVLLRQLWNGLEQTPGVKVYGPPPGTPR
                     TATLSFAVTGHTSAAVAAFLAARGVFVSHGDFYATTAVRVLGHEHDGLVRAGCAAYTT
                     GEEVARLVEGVRQLLRA"
BASE COUNT          276 a          620 c          642 g          263 t
ORIGIN      
        1 cttggtccga ccgagtagcc gatcccaaaa aggaaaagaa gaaataagat tgactgagcc
       61 gctcgagcct gcccggctcc tggcaatcgc ggcgcgtatc gaaatccatg acggtcacgc
      121 gggcggcgcc gaggtcgctg aaaatccgct cgtagcgcgg cccggtctca tgcacgcggc
      181 tcgcggtggg tatgacgacg atgtccgcct gtccgccccc cgaagcgctg acgaagcgct
      241 cgagaatgcg gcgatcgttc tccttattct ccgcgccacc gattgcgatg atccatccgc
      301 gccgctcacc ttgcggaact ctgctgggca tttcggtccc tgactcctta taaagacagc
      361 tccggcgcgt gaaggtaaca cgccgggccg ggggccgggg ggctgccgct tgcgcactgt
      421 gaccgcgcgc ccagaaccgc aggtaagctg cgctccgccg acgcagcacc aggagcccgg
      481 cgccgtgtcg ggctcgtttt ctcaagcgcg caggagttgc cgtactccct cgacgaggcg
      541 cgcgacctcc tcgccggtcg tatacgccgc gcagccggcg cgcaccagcc catcgtgctc
      601 gtgacccagc acgcgcaccg ccgtggtggc gtagaagtcc ccgtgcgata cgaagacgcc
      661 gcgcgcggcg aggaacgcgg cgaccgcggc gctcgtatgc cctgtcaccg cgaacgacag
      721 ggtggcggtg cgcggcgtgc cgggcggcgg gccgtaaacc ttgaccccgg gcgtctgctc
      781 gagcccgttc cacagctgcc gcagcagcac ctcgccgcgc tcgtgcaggg tctgcgacag
      841 ccgcgcgagc cgctcgcgcc gcggcaggcg ctcacccgcg agcgaggcga ggaagtcgac
      901 ggtcgctgca gcgccgacga ttccctcgtg attgagtgtg ccggtctcga gcagctcggg
      961 gggctcgccc ggcgcgggtg cgagcttggg cgggtcgagc gccgcaagca gctcacgccg
     1021 gccccacagc actccgacgt gcggaccgta gaacttgtag gccgagcagg cgaagaagtc
     1081 gcagtcccag gcgcgcacgt ccggcatctg gtggctcgcg tagtgcacgc cgtcgatgaa
     1141 tacccacgcg ccgaccgcgt gcgcgcgcgc ggtgagctgc gccacgtcat tgatggtgcc
     1201 gagggcgttc gaggcggcac cgagggcgag cagccgcacg ccgggggtga gcttcgccgc
     1261 ggcgtctgcc tcgtcgagcc gcccatcctc gcgccgcatg cggatcgcgc ggatctcgag
     1321 cccacgctcg cgcgccaggg cgcgccaggt atcggcattg gcgtggtgat cgagctcggt
     1381 gaccacgacg acgtcgcccg cgcgccagcc gcgtccgagc gtgcgcccga ggtggaaggt
     1441 gagcgtcgtc atgttggcgc cgaagacgat ctcgtccgca gagcccccca gccagtccgc
     1501 gagcgcggcg cgcgcggcgc cgagcgccag gtcggtctcg tggctcgagg ggaaatccca
     1561 gtgagtgttg gcgttgtggt tgaggagata gtccgtcatc gcggccgcga ccgcgcgcgg
     1621 tacctgggtg ccgcccgggc cgtcgaaata cgccacggga aaaccgttgt aagtgcgctc
     1681 gagcgctgga aaatggctgc ggatctcgtc gacgccggcg acgtgggtca tgggagcctc
     1741 ggcagggcgg tgtaccgaca ctatagcgcg ctcagccgcg cgccgtgctc gagcgggctt
     1801 g
//
LOCUS       DATLKL010000607         2394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_165316, whole genome shotgun sequence.
ACCESSION   DATLKL010000607 DATLKL010000000
VERSION     DATLKL010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165316"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VM713_08135"
     CDS             <1..506
                     /locus_tag="VM713_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84268.1"
                     /translation="GYTHVLTMDSDGQHPAECIGSFMSASAAAPEAMILGVPVFDSSA
                     PRLRVRGRRIGNWWTNLETLWSGIADTLFGFRVYPIAPLLEVMQRTRWMRRFDFDAEA
                     VVRLSWRGLPVRNLAAPCRYFSAAEGGVSHFNYWRDNLLLTWMYTRLLLGFLVRLPVL
                     VARRIAR"
     gene            645..938
                     /locus_tag="VM713_08140"
     CDS             645..938
                     /locus_tag="VM713_08140"
                     /inference="COORDINATES: protein motif:HMM:NF006617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HVH84269.1"
                     /translation="MSPLRQPAGSAGIDALTRELAGLIVAALNLEVAAADIGSDQPLY
                     GEGLGLDSIDILEIALVVSKQYGVQLRADSEDNERIFASLRHLAEYVNTHRTK"
     gene            985..2307
                     /locus_tag="VM713_08145"
     CDS             985..2307
                     /locus_tag="VM713_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVH84270.1"
                     /translation="MLAYRAGAPILVRQFLADVERVGAALGAGGHVLNVCSDRYRFMV
                     GFAAGLRAGQVSLLPSTRTPEVIAQLKSFAPDTVCLTDEDACDLALPLVRFPEADSAA
                     RAAANRPVGTVPRVDPGRRAAWVFTSGSTGTPVPHQKTFGRLIECMRVEAQRLGLAAG
                     VSHAIVATVPPQHMYGFEASVLLPLASGSALVAERPFYPADICAALAAVPRPRVLVTT
                     PIHLRALMAASLAIPDTDLIVSATAPLSVQLAAEVEQRCATRLLEIYGSTETGSIAVR
                     HTTTTAEWRLFPDVVLEPRDGEIWAHGGHIEQATRLCDVIELTGADRFLLHGRLADLV
                     NIAGKRSSLAYLNHQLNSIPGVLDGAFFHGEAAAAVAAPTRVGACVVAPGLDAAAIAA
                     ELRRRIDPAFLPRPLLLVKSLPRTSTGKLPQEALRSLAIAQQKPGPAR"
BASE COUNT          327 a          887 c          782 g          398 t
ORIGIN      
        1 cgggatacac ccatgtgctc acgatggact ccgacggcca gcatcccgcg gagtgcatcg
       61 ggagcttcat gagtgcctcc gcggcggcgc ccgaggccat gatcctcggc gtgccggtgt
      121 tcgactcgag cgcgccgcgc ctacgggtgc gcgggcggcg cattggcaac tggtggacca
      181 acctcgagac gctgtggtca gggatcgcgg acacgctctt cggctttcgc gtctatccaa
      241 tcgccccgct cctcgaggtg atgcagcgga cgcgctggat gcgacgcttc gacttcgacg
      301 ccgaggcggt ggtgcgcctc agctggcgcg gtctaccggt gcgcaacctc gcggcaccct
      361 gccgctactt cagtgccgcg gagggcggcg tgtcccattt caattattgg cgcgacaacc
      421 tgctgctcac ctggatgtat acgcgcctgt tgctcggttt cctggtgcgg ctgccggtgt
      481 tggtggcgcg gcgcatcgcg cgctgacgcc gccgtggcgc gcagttgcgg cgccccgctg
      541 cggcggcacc gtgcggttcc ctcggctggt tgctgtgcga ttcgcccttg tataatgccg
      601 cccagcgttt ttactcctta cttaatcagc aaatgtccga caacgtgtca cccctgagac
      661 agccggccgg ctctgccggt atcgacgctc tcacccgcga gctcgccggc ctgatcgtgg
      721 cagcgctcaa cctcgaagtt gcggcggcgg atatcgggtc cgaccagccg ctctacggcg
      781 agggactcgg cctcgactcg atcgacatcc tggaaatcgc gctggtggtg tccaagcagt
      841 acggcgtgca gctgcgcgcc gacagcgagg acaacgagcg catcttcgcc tcgctccgcc
      901 acctggccga atacgtcaac acgcacagga cgaagtaatc cactgagcag cctgccgctg
      961 atcgcccatg catcggccga cgcggtgctc gcctaccgcg ccggggcgcc gatcctcgtc
     1021 agacagtttc tcgccgacgt cgagcgcgtc ggcgccgccc tcggcgccgg cggccacgtg
     1081 ctcaacgtct gcagcgaccg ctaccgcttc atggtgggct tcgccgcggg cctccgcgcc
     1141 ggccaggtga gtctgctgcc ctcgacgcgc acccccgagg tcatcgccca gctcaagtcc
     1201 ttcgcgcccg acacggtttg cctgaccgac gaggacgcgt gtgacctcgc tctgccgctg
     1261 gtgcgcttcc ccgaggccga ctcggccgcg cgggccgctg cgaatcgacc cgtcggcacg
     1321 gtaccgcgcg tcgatccggg gcgtcgggct gcctgggtgt tcacttccgg ctccacgggg
     1381 acgccggttc ctcaccagaa gaccttcggc aggctcatcg agtgcatgcg cgtggaagcg
     1441 cagcgcctgg gcctcgcggc cggggtgtca cacgccatcg tcgccacggt acccccgcag
     1501 cacatgtatg gtttcgaggc gagcgtgctg ctgccactcg cgagcggcag cgcgctcgtc
     1561 gcggagcggc cgttctaccc tgcggacatc tgtgcggcgc tggccgcggt gccgaggccg
     1621 cgggtgttgg tcacgacacc gattcacctg cgcgcgctca tggcagcaag cctcgccatt
     1681 cccgacaccg acctcatcgt gtcggcgacg gcaccgctct ccgtgcagct cgccgcggag
     1741 gtggagcagc gctgcgccac gcgcctgctc gagatctatg gctcgaccga gaccgggtcg
     1801 atcgccgtgc gccacacgac caccactgcc gagtggcggc tgttcccgga cgtcgtgctc
     1861 gagccgcgcg acggggaaat ctgggcgcac gggggccata tcgagcaggc aacgcgcctc
     1921 tgcgacgtga tcgagctgac cggcgccgat cgcttcctgc tgcacggccg cctcgccgac
     1981 ctcgtcaaca tcgccggcaa gcgcagctcg ctcgcctatc tcaaccatca gctcaactcc
     2041 atccccggag tactcgacgg tgctttcttc cacggggagg cagcagccgc cgtcgccgca
     2101 ccgacccggg tcggcgcctg cgtcgtggct ccgggcctcg acgccgcggc catcgccgcg
     2161 gagctccggc ggcgcatcga tccggcgttc ctgccacgcc cgctgctcct ggtgaagagt
     2221 ctgccgcgca ccagcaccgg gaagcttccc caggaggcgc tgcgctcgct ggccatcgcc
     2281 cagcagaagc cgggccccgc gcggtgaccc aggcatgcgc gcgctcacga ccctcaggat
     2341 tgaccccggg cacccggcac tcgccgggca cttccccggg gctccggtgc tccc
//
LOCUS       DATLKL010000608         1767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_165342, whole genome shotgun sequence.
ACCESSION   DATLKL010000608 DATLKL010000000
VERSION     DATLKL010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165342"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="VM713_08150"
     CDS             <1..1576
                     /locus_tag="VM713_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVH84271.1"
                     /translation="VAATPPSGTVGPVELRVERGATRYVVETVGSNLTQDPAVQGLAL
                     NFRDISERKALEEQLRQLAFHDPLTLLANRNLFRDRVQHALAIAQRGPNCLAVMFLDL
                     DNFKNINDSLGHDAGDRLLQAVAHRILQTTRTSDTVERLGGDEFAVLMEGVERSSEVG
                     RVADALIAALNVPFQLDGREVRVGASVGVAISATEAGAEALLSNADIAMYHAKAGGKN
                     RHVAFQPQMQALLHERLRLEADISRALANQEFFIEYQPIVDLSTRSLLGVEALVRWRH
                     PEAGVLMPGRFIQVVEESGQIVKLGRWVLKQACRDLCLWRRSIAGGAGLRLAVNISGR
                     HLQHGELVHDVAQALAESGLEPGNLVIQLTESTIMYNTDANLERFHHLKALGVRLAID
                     DFGTGYSSLSYLHRFPIDILKIDRSFVGSLTNSDNGPELARAVITLGETLGLETVAEG
                     IELEPQVASLVALGCVAGQGFLFAKAGSLEHLSKSPFVARRNALWNAQTRSEDLSPTG
                     RFEALKSVPGRSAGAA"
BASE COUNT          265 a          629 c          606 g          267 t
ORIGIN      
        1 cgtcgctgcg acgccgccct cgggcacggt tggtccggtc gagctgcggg tcgagcgcgg
       61 cgccacgcgt tacgtggtcg agaccgtcgg cagcaacctg acgcaggacc ccgcggtgca
      121 gggactggcg ctcaacttcc gcgacatcag cgagcgcaag gcgctcgagg agcagctgcg
      181 ccagctggcc ttccacgacc cgctcacgct gctcgccaac cgcaatctgt tccgcgaccg
      241 cgtgcagcac gcgctcgcca tcgcgcagcg cggcccgaat tgcctcgcgg tcatgttcct
      301 cgacctcgac aacttcaaga acatcaacga ttccctcggg cacgacgcgg gcgaccggct
      361 gctgcaggcg gtggcgcacc ggatcctgca gaccacgcgc acctccgaca ccgtggagcg
      421 gctcgggggc gatgagttcg cggtgctgat ggaaggcgtc gagcgctcga gcgaggtggg
      481 acgggtggcc gatgcgctga tcgcagccct caacgtgcca tttcaactcg acggccggga
      541 agtgcgcgtc ggggcgagcg tcggtgtggc gatctccgcc accgaagccg gggccgaggc
      601 gctgctcagc aacgccgaca tcgccatgta tcacgccaag gccggcggca agaatcgcca
      661 cgtcgccttc cagccgcaga tgcaggcgct gctgcacgag cggctgcggc tcgaagccga
      721 catctcgcgc gcgctcgcca accaggaatt cttcatcgag tatcagccga tcgtggactt
      781 gagcacccgc agcctcctcg gcgtcgaggc gctggtgcgc tggcgtcatc ccgaggccgg
      841 cgttctcatg ccggggcgct tcatccaggt cgtcgaggag tccggccaga tcgtcaagct
      901 cgggcgttgg gtgctgaagc aggcgtgccg cgacctgtgc ctgtggcgcc ggtcgatcgc
      961 cggcggggcc gggctgcggc tcgcggtgaa catctccggc cgccacctgc agcacgggga
     1021 gctggtgcac gacgtcgcgc aggcgctggc cgaatccggg ctcgagccgg ggaacctcgt
     1081 catccagctc accgagagca cgatcatgta caacaccgac gcgaacctcg agcggttcca
     1141 ccacctcaag gcgctcggcg tgcggctcgc gatcgacgac ttcggcacgg gctattcgtc
     1201 gctgtcctac ctgcatcgct tcccgatcga catcctgaag atcgaccgct ccttcgtcgg
     1261 cagcctgacc aattccgaca acgggccgga gctcgcgcgc gcggtcatca cgctcggtga
     1321 gacgctcggc ctcgagacgg tagccgaggg catcgagctc gagccacagg tcgcctcgct
     1381 cgttgcgctc ggctgcgtgg ccggacaggg attcctgttc gccaaggcgg gctcgctcga
     1441 gcatctttcc aagagcccgt tcgtggcgcg gcgcaacgcg ctgtggaacg cacagacgcg
     1501 cagcgaggat ctgtcgccga ccgggcgctt cgaggccctg aagtccgtcc ccggacgcag
     1561 cgccggcgcc gcctgaagtc tcgcgcgcgc gcggtgcgcg ccggccgccg cagccggccg
     1621 gtatagaatc gccgtatggt tcccgccgtc gacgtcgccg cgctgtttgg accggcgggg
     1681 cgtgcgcgtg cggccgccga cgcggccatc gcggcggcag ccgccgacat aggcttcctc
     1741 accatcaccg ggcttccggc gtgcgcg
//
LOCUS       DATLKL010000609         3677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_154316, whole genome shotgun sequence.
ACCESSION   DATLKL010000609 DATLKL010000000
VERSION     DATLKL010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154316"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VM713_08155"
     CDS             complement(<1..359)
                     /locus_tag="VM713_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH84272.1"
                     /translation="MSARARNRYDAIVVGAGHNGLVAACYLARAGLDVLVLERNPYIG
                     GAAVSRRLYDGFTYSNCSYVCSLLRTEIIRALELPRYGLQIIPYEGGCTLMRGGGHLA
                     LYDNHDALRREIARHSRR"
     gene            complement(356..1702)
                     /locus_tag="VM713_08160"
     CDS             complement(356..1702)
                     /locus_tag="VM713_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH84273.1"
                     /translation="MTKNHEHFRRALTRLPLGVASNFRYWGEERTIYVKEGRGARITD
                     LDGNVYVDYRMGYGPSILGYADARVDAAAREGMKVGGVFALSTEAEYEVAERIARMVP
                     AAELVRFSNSGTEAVMAALRLARAYTKRDDYVILEGSYHGLFDAAMWYTPMDKWQQVG
                     DPEVHPYSEGVPLATRNFAHFVQANDANQLEDVLKRHGDRIACLLVEPIMGNCLGIAA
                     EPAYVRVARELCDRHGVVMIIDEVKTGFRVARGGVQQLYGVRADLCTFAKALANGYPI
                     SVLAGREDIMRKLGRGVAHGGTYTAHPVSLAAASKTLEILAETDALERIAAYGTRLRL
                     GLSQILKSRGIPHSFVGHPSMSGLYFAHDPPRTYRDWKSSDYTFYDATAKVLHDECIL
                     CEPDSREPWFVSAAHDDSCLADTLGAFEIAIDRTLDALPGARRGRLFGLMRGEGAT"
     gene            1839..3008
                     /locus_tag="VM713_08165"
     CDS             1839..3008
                     /locus_tag="VM713_08165"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HVH84274.1"
                     /translation="MTSPIPRALDDAPTTAPHSLPAWVYNHPEMTRLELERILKPSWQ
                     IACHVSQIPSAGDYVTFELGNDSVIVLRDAGGAIRALHNTCRHRGTRLLEGSGRCLGR
                     ITCPYHGWTYRYDGSLLATPARDSFPDLDLRQHGLGTVRLEVALGFVWVCLGADAPPP
                     PSQIWAPIVEELAPYRLEELVPTQPLFREEWQADWKIAMDNYLESYHVPIGHPGLSRM
                     FTPDYEDQRSVPGIARGISWMRATPSSRWSERLYQKYVGEVTAAHLPERERRSWRFYS
                     CLPNHGIDIFPEQVDFFQVLPRGPGKALIRSASFGLPDERREMRLVRWLGNRINMQVN
                     DEDRALCERLQRGIADSHYRPGPLSQIETWMLAFHNLLRARIPEVRLPSAPARFS"
     gene            3016..>3677
                     /locus_tag="VM713_08170"
     CDS             3016..>3677
                     /locus_tag="VM713_08170"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVH84275.1"
                     /translation="MRRVPSSTAPVVEGVRVQARPLRVRARQRLIDAAISALHIYGPS
                     RTTVSRVVAIAGLSPGIVRFYFNSKAAMLVAALEYLASEFEQRLLKPVAALKHSPVVA
                     LGLLVELYLDPEIASPRKVSVWYSFWGEASSRQEYLEICGEKDEAFAQLVRELTRELI
                     AQTGAAHLDADGVALGLIGVLEVLWQGIAFQSEERIDRRALVARALAYLRSVFPGQFP
                     AAA"
BASE COUNT          586 a         1278 c         1211 g          602 t
ORIGIN      
        1 cggcgcgaat ggcgcgcgat ctcgcgccgc agggcgtcgt gattgtcgta gagcgcgagg
       61 tgcccgccgc cgcgcatcag cgtgcagccc ccctcatagg ggatgatctg caatccataa
      121 cgcggcagct cgagcgcacg gatgatctcc gtgcgcagca ggctgcagac gtacgagcag
      181 ttggaatagg taaagccgtc gtagaggcgg cggctcaccg ccgcgccgcc gatataagga
      241 ttgcgctcga gcaccagcac gtcgagacca gcgcgggcga ggtagcaggc ggcgaccagt
      301 ccgttgtgcc ccgcgccaac tacgatcgcg tcgtagcggt tgcgcgcgcg tgcgctcaag
      361 tggcgccttc ccctcgcatg agcccgaaca gccgcccgcg ccgtgcaccc ggcagcgcat
      421 caagcgtgcg gtcgatcgcg atctcgaagg cccccagcgt gtccgcgaga catgagtcat
      481 cgtgcgcggc ggacacgaac cacggctcgc gcgagtcggg ctcgcacagg atgcactcgt
      541 cgtgcagcac cttcgccgtg gcatcgtaga aggtgtagtc gctgctcttc cagtcgcggt
      601 aggtgcgcgg cggatcatga gcaaagtaca ggccactcat cgacggatga cccacgaagc
      661 tgtgagggat gccgcggctc ttgaggatct ggctcagccc caagcgcagg cgcgtgccgt
      721 atgcagcgat gcgctcgagc gcgtccgtct ccgccagaat ctcgagtgtc ttggaggccg
      781 ccgccagcga caccggatgc gccgtatacg tgccgccatg ggcgacgccg cggccgagct
      841 tgcgcatgat gtcctcacgc ccggcgagca ccgagatcgg atagccgttg gcgagcgcct
      901 tggcgaaggt gcaaagatcc gcccgcacgc cgtagagctg ctgcaccccg ccgcgcgcga
      961 ctcgaaatcc ggtcttcacc tcgtcgatga tcatcacgac accgtggcgg tcgcacagct
     1021 cgcgcgccac gcgcacgtag gcgggctccg cggcgatccc caggcagttg cccatgatcg
     1081 gctcgaccag caggcaggcg atgcggtcgc cgtggcgctt gagcacgtcc tcgagctggt
     1141 tggcatcgtt cgcctgcacg aagtgggcga agttgcgcgt cgcgagcggc acgccctcgc
     1201 tgtaaggatg tacttccggg tcgcccacct gctgccactt atccatcggc gtgtaccaca
     1261 tggccgcatc gaacagcccg tggtagctgc cctcgaggat cacgtagtca tcgcgcttgg
     1321 tgtaggcgcg cgccaggcgc agcgccgcca tcaccgcctc ggtaccggag ttggagaaac
     1381 gcaccagctc ggccgccggg accatccgtg cgatgcgctc ggcaacctcg tattccgcct
     1441 cggtcgagag cgcaaacacg ccgccgacct tcatgccctc gcgcgccgcc gcatccaccc
     1501 gcgcatcggc gtagccgaga atcgaaggcc cgtagcccat ccgatagtcg acgtagacgt
     1561 tgccgtcgag gtcggtgata cgcgcgccgc gcccttcctt cacgtagatg gtgcgctcct
     1621 cgccccagta gcggaaattc gaagcgaccc cgagcggcag ccgcgtgagc gcccgccgga
     1681 agtgctcgtg gttcttcgtc atggttctcg ccacggctgc cgcccccaaa tgtggacccc
     1741 gccgagctcg gcggggctct tgccatgcga ctatatgagc atatagtttt ttgcgctaaa
     1801 attaaacagc cgtataaacg cagcccggag ggctgaccat gacttcgccc atcccccgcg
     1861 cgctggacga tgcccccacc accgcccccc actcgctgcc ggcgtgggtg tacaaccacc
     1921 ccgagatgac gcgcctcgag ctcgagcgga tcctcaagcc gagctggcag atcgcctgcc
     1981 acgtcagcca gatccccagc gcgggagact acgtcacctt cgagctgggc aacgacagcg
     2041 tgatcgtgct gcgcgatgcg ggcggcgcga ttcgcgcact gcacaatacc tgccgccatc
     2101 gcggcacgcg cctgctcgag ggatccggcc gctgcctggg gcgcatcacc tgcccgtacc
     2161 acggctggac ttaccgctat gacggctcac tgctcgccac cccggcccgc gacagctttc
     2221 ccgacctcga cctgcgccag cacgggctcg gcacggtacg cctcgaggtt gcgcttggct
     2281 tcgtctgggt ctgtctcggc gccgatgctc cgcccccgcc gtcgcagatc tgggcgccga
     2341 tcgtagagga gctcgcgccc tatcggctcg aggagctcgt gccgacgcag ccgctgttcc
     2401 gcgaggagtg gcaggccgac tggaagattg cgatggataa ttacctcgag tcctaccacg
     2461 tgcccatcgg ccatccgggc ttgagccgca tgtttacacc cgactacgag gatcagcgca
     2521 gcgtgcccgg catcgcccgc ggcatcagct ggatgcgcgc gacgccctcg tcgcgctgga
     2581 gcgagcgcct ctatcagaag tacgtgggcg aggtgacggc ggcgcatctc ccggagcgcg
     2641 agcgccgcag ctggcggttc tacagctgcc tgccgaatca cggcatcgac atctttcccg
     2701 agcaggtgga tttctttcag gtgctgccgc gggggcctgg caaggcgctg atccgcagcg
     2761 cctccttcgg cctgccggac gaacgacgcg agatgcgcct ggtccgctgg ctcggaaacc
     2821 gcatcaacat gcaggtcaac gacgaggacc gcgcgttgtg cgagcggctg cagcgcggca
     2881 tcgccgacag ccactaccgg cccggaccgc tgtcgcagat cgagacctgg atgctcgcgt
     2941 tccacaacct gctgcgcgcg cggatccccg aagtgcgcct gcccagtgcg ccggcgcgct
     3001 tcagttaggg cgggggtgcg ccgggtaccg agctccaccg cgccggtggt cgagggcgtt
     3061 cgcgtgcagg cgcgaccctt gcgcgtgcgc gcacggcagc gcctgatcga tgcagccatc
     3121 tcggcgctgc acatctacgg cccttcgcgc acgacggtga gccgggtggt ggcgatcgcg
     3181 gggctctccc ccggcatcgt gcggttctat ttcaactcga aggcggcgat gctggttgcg
     3241 gctctcgagt atctcgcctc cgagttcgag cagcggctgc tgaagccggt cgccgcgctc
     3301 aagcactcgc cggtcgtggc gctgggactg ctggtcgagc tctatctcga tcccgagatc
     3361 gccagcccgc gcaaggtttc cgtgtggtac tcgttctggg gcgaggcgag ctcgcgccag
     3421 gagtacctgg agatctgcgg cgagaaggat gaggccttcg cccaactggt gcgcgagctc
     3481 acgcgcgagc tgatcgcgca gacgggcgcc gcgcacctcg atgccgacgg ggtggcactc
     3541 ggcttgatcg gcgtgctcga ggtgctgtgg cagggaatcg cctttcaaag cgaggagagg
     3601 atcgaccggc gtgccctggt ggcgcgtgcc ctcgcctacc tgcggtcagt cttcccggga
     3661 caattccccg ccgccgc
//
LOCUS       DATLKL010000610         2670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_176501, whole genome shotgun sequence.
ACCESSION   DATLKL010000610 DATLKL010000000
VERSION     DATLKL010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176501"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VM713_08175"
     CDS             <1..307
                     /locus_tag="VM713_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="poly-beta-hydroxybutyrate polymerase"
                     /protein_id="HVH84276.1"
                     /translation="LTRSSDYTFLLTSGGHNAGIVSGPVHPKRRHRLLTWSDATTTLT
                     PDAWHKRAPLHEGSWWPAWEKWLAEHSAERQLPARPVAATGADGAALDEAPGSYVRG"
     gene            383..1132
                     /gene="phbB"
                     /locus_tag="VM713_08180"
     CDS             383..1132
                     /gene="phbB"
                     /locus_tag="VM713_08180"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HVH84277.1"
                     /translation="MSSKTNRIAYVTGGMGGIGTAICRRLAKEGYIVIAGCGPGSQRK
                     DRWLADMRAEGLNVHASEGNVSDWDSTKRAFEVARAEVGPIDILVNNAGITRDGVFQK
                     MTNEMWHAVIETNLNSLFHVTKQVIDGMLERGWGRIVNISSVNGQRGQFGQTNYSTAK
                     AGIHGFTMALALELAGRGVTVNTVSPGYIGTEMVKAIKPERLEKIIAGIPVKRLGTPE
                     EIASIVAWLVSDESAFATGADFSLNGGLHMG"
     gene            1152..1772
                     /locus_tag="VM713_08185"
     CDS             1152..1772
                     /locus_tag="VM713_08185"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HVH84278.1"
                     /translation="MKICVVGAGAIGGFLGTRLALDAGNAVTALARGTTLVSLRDHGW
                     RLRQNGELLQAPTAAASDDPRDLGSQDLVILALKAQVLPGLAPSLTPLISADTIVMPA
                     MNGVPWWFGAGVAALATRPLESVDPGGGIAAVVPLKSVVGCVVHASASTSEPGVTIHR
                     MGRGLIVGEPAGGESARVARLGETLTRAGFEVSCSPRIRYDVWYKL"
     gene            1779..2141
                     /locus_tag="VM713_08190"
     CDS             1779..2141
                     /locus_tag="VM713_08190"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HVH84279.1"
                     /translation="MTMNPVSALTGATTDRLLDDELVRIFCARAMQEAAAVGARIGCD
                     VRESADDRQAVTRKLGVFKTSMLQDVEASRTLELDALVGAVREIGQRVGVATPMIDAL
                     FGLTRLFGQVRGLYPLQR"
     gene            complement(2151..>2670)
                     /locus_tag="VM713_08195"
     CDS             complement(2151..>2670)
                     /locus_tag="VM713_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84280.1"
                     /translation="LSVGEAGRLLGLAHLIAGTGGTLLAGALMALPQARDPRFVARLL
                     AWVTALATIPSLLLYWVPSAESATLWLWIFVPAVYFYIGPILGLLQNVMPANMRASAC
                     AILLFSANVANLILAPQLIGFLSDWFAASFGAGRESLRWALLLLATTGLWAAWHLRVA
                     AKTIRADEARAT"
BASE COUNT          449 a          925 c          920 g          376 t
ORIGIN      
        1 cctgacgcgc agctccgatt acacgtttct cctgacgagc ggcggtcata acgccggcat
       61 cgtcagcggg cccgtgcacc cgaagcgtcg gcaccgcctg ctcacgtggt cggacgcgac
      121 cacgacgctg acgccggacg cgtggcacaa gcgcgcaccg ctccacgaag gctcctggtg
      181 gccggcgtgg gagaagtggc tggcagagca ttcggccgag cggcagctgc cggcacgtcc
      241 ggtggcggcc accggggctg acggcgcggc actggacgag gctcccggaa gctacgtgcg
      301 cggctgagcg cgccgctgcc gcggcaatgg acgcccggcg aaaatcgcgg tactaatgtc
      361 cgccagaaac ggtggacagg ccatgagcag caaaactaac cgcatcgcat acgtcaccgg
      421 cggcatgggt ggcatcggca cggcgatctg ccggcgcctc gccaaagaag gctacatcgt
      481 aatcgccggc tgtggccccg gctcacagcg caaggatcgc tggctcgccg acatgcgcgc
      541 cgagggtttg aacgtccatg cctccgaggg caacgtctcg gactgggact cgaccaagcg
      601 cgccttcgag gtggcgcgcg cggaggtcgg cccgatcgac atcctcgtga acaacgccgg
      661 catcacccgc gacggcgtct tccagaagat gaccaacgag atgtggcacg cggtgatcga
      721 gaccaacctc aactcgctct tccacgtcac caagcaggtg atcgacggca tgctcgagcg
      781 cggctggggg cggatcgtca acatctcgtc cgtgaacggc caacgcggcc agttcggcca
      841 gacaaactac tcgaccgcca aggcgggcat tcacggcttc acgatggcgc tcgcgctgga
      901 gctcgccgga cgcggcgtca ccgtgaatac ggtctccccc gggtacatcg gcaccgagat
      961 ggtgaaggcc ataaagcctg agcgcctcga gaagatcatc gcggggattc ccgtcaagcg
     1021 gctcggaacg ccggaggaga tcgcctcgat cgtcgcctgg ctggtgagcg atgagtccgc
     1081 cttcgccacg ggcgccgact tctccctgaa cggcggcctg cacatgggtt agggcgcggg
     1141 gccgcgcgca cgtgaagatc tgcgtcgtcg gcgcgggagc gatcggcggc ttcctcggta
     1201 cccgtctcgc gctcgatgca ggcaacgctg tcaccgcgct cgcgcgcggc acgacgctcg
     1261 tctcgctccg cgaccacggg tggcggctcc gtcagaacgg cgagctgctg caggccccca
     1321 ccgcggcggc ctcggacgac ccgcgcgacc tgggcagcca ggatctggtg atcctcgccc
     1381 tcaaggcgca agtcctgccg gggcttgccc cctcactcac cccgctcatc tccgccgaca
     1441 cgatcgtcat gccggccatg aacggcgtgc cttggtggtt cggagccggg gtcgccgcgc
     1501 tcgcgacccg gcccctcgag agcgtcgatc cggggggcgg catcgccgcg gtcgtcccgc
     1561 tgaagagcgt ggtcggctgc gtggtacacg cgagcgccag caccagtgag cctggcgtca
     1621 ccatccaccg catgggccgc ggccttatcg tgggcgagcc cgcgggcggt gagtccgcgc
     1681 gcgtcgcacg gctcggcgag accctcaccc gcgccggctt cgaggtcagc tgctcgccgc
     1741 gcatccgcta tgacgtctgg tacaagctct gaggcaacat gaccatgaac ccggtctcgg
     1801 ccctcacggg cgcgaccact gacaggctgc tcgatgatga gctggtgcgc atcttctgcg
     1861 cccgcgccat gcaggaggcc gccgcggttg gcgcgcgcat cggctgcgac gtgcgcgagt
     1921 ccgccgatga ccgccaggcc gtgacgcgca agctcggagt cttcaagact tccatgctcc
     1981 aggacgtgga agcgagccgc acgctcgagc tcgacgcgct ggtgggagcg gtgcgcgaga
     2041 tcggtcagcg cgtcggcgtg gcgacaccga tgatcgatgc gctctttggc ctcacgcgac
     2101 tcttcggtca ggtgcgcggc ctgtacccgc tgcagcgata ggcaatgctc tcaggtcgca
     2161 cgcgcctcgt ccgcgcggat ggtcttggcc gccacgcgca ggtgccaagc ggcccacaga
     2221 ccggtggtcg cgagcagcag cagcgcccag cgcagtgact cccgcccggc accgaaggag
     2281 gccgcgaacc agtcgcttaa gaagccgatc agctgcggcg cgaggatgag gttcgcgacg
     2341 ttggcgctga acagcaggat ggcgcaggcg ctggcgcgca tgttcgcggg catcacgttc
     2401 tgcaggagcc cgaggatcgg gccgatgtag aagtacaccg cgggcacgaa gatccacagc
     2461 caaagagtcg cggactcggc cgacggcacc caataaagga gtagcgacgg aatcgtcgcg
     2521 agcgccgtca cccacgcgag gaggcgtgcg acgaagcgcg gatcgcgcgc ctgcggcaag
     2581 gccatcagcg ctcccgcgag cagcgtgccg ccggttcctg cgatgaggtg cgcgagcccg
     2641 aggagcctgc ccgcctcacc caccgagagc
//
LOCUS       DATLKL010000611         2641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_154320, whole genome shotgun sequence.
ACCESSION   DATLKL010000611 DATLKL010000000
VERSION     DATLKL010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154320"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VM713_08200"
     CDS             <1..355
                     /locus_tag="VM713_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HVH84281.1"
                     /translation="DGVPGLQARARDGTWVDVPPMEGALAVNFGQVLERWSGGRIRAT
                     EHRVIGSARERFSIPFFYEARADAEIRPLPLDDPESFAPFLYGDYLWERITAFVEFRG
                     MERQRDARGAAVVLP"
     gene            460..1143
                     /locus_tag="VM713_08205"
     CDS             460..1143
                     /locus_tag="VM713_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84282.1"
                     /translation="MGRYFTLAALLTLAGCYNMPGGDVTASGSGSGTNTVNGSIHVPA
                     GTHSGAVGTVNGSIRIDDDATVASAGTVNGSVALGAHATADSVGTVNGSITLGEGAHV
                     ARDVSTVNGSMTLKSGAEVSGPLSNVNGRIALTAAHVAGGLHTVDGDIDVGGGSHVEG
                     GILVKHGGGGWFNFSTHKPRIVIGPGAVVQGDLRFERAVELYVSDSATTGPITGATAI
                     RFSGASPQG"
     gene            complement(1154..1804)
                     /locus_tag="VM713_08210"
     CDS             complement(1154..1804)
                     /locus_tag="VM713_08210"
                     /inference="COORDINATES: protein motif:HMM:NF020348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HVH84283.1"
                     /translation="MSPFPEDIYTEQEADPDTLANLGPLRRLAGTWESRAGIDVNPKP
                     EGPQRREYSERIVLEPIDAQSNGPQLFYGLRYHTRILASDEDATFHDQTGYWLWEPAT
                     GLVLQTLSIPRGQVGIAAGHATADATELVLTAQRGQTEYGICSTTFLEYAFRTDSYRI
                     EVVFNPDGSWSYVLDTMLKVRGREEPFLHRDRNTLVKIAEPQPNPLALLRAKAAGR"
     gene            1910..2299
                     /locus_tag="VM713_08215"
     CDS             1910..2299
                     /locus_tag="VM713_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HVH84284.1"
                     /translation="MSPELRYLALVATLTGLLWVPYILNEIAVRGLLDAVGYPANPKP
                     LAPWAQRLKGAHYNAVENLVVFAALVLAAVAAGVHHPAVATAAAIYFWARLVHAVAYT
                     LAVPWVRTLSFLVGFAMQMWVAAVILL"
     gene            complement(2286..>2641)
                     /locus_tag="VM713_08220"
     CDS             complement(2286..>2641)
                     /locus_tag="VM713_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84285.1"
                     /translation="EAEARKLLRRFPGIGAPGADRILLFGALAPVAAVPSSSPHVLVR
                     LASGREAGKYTATYARAQQLIEAQVRATLSARARAYLLLQRHARQLCKRAQPKCGECP
                     VSGDCAYFGQLTKAE"
BASE COUNT          386 a          875 c          949 g          431 t
ORIGIN      
        1 ggacggagtg ccggggctgc aggcgcgcgc ccgcgacggc acgtgggtcg acgtaccgcc
       61 gatggagggc gcgcttgcgg tgaatttcgg ccaggtactc gagcgctgga gcggcggccg
      121 catccgcgcc accgagcacc gggtcatcgg cagcgcacgc gagcgcttct cgattccctt
      181 cttctacgag gcgcgcgccg acgccgagat ccgccccctg ccgctcgatg acccggagag
      241 cttcgcgccg ttcctgtacg gcgactacct gtgggagcgg atcaccgcgt tcgtcgagtt
      301 ccgcggcatg gagcggcagc gggatgcgcg cggcgccgcc gtagtgcttc cgtgacgctg
      361 ctcgccgcgc ccggcagtgc ggcgggcgcg gcgtggctcc aaggagtgga cagctcgcga
      421 cggcggcggt agcttcctcc ggccgccaag gagaccgcca tgggacgtta cttcacgctt
      481 gccgcactgc tgacactcgc tggctgctac aacatgcccg gaggcgacgt caccgccagt
      541 ggcagcggca gcggaacgaa caccgtcaac ggctcgatcc atgtgcccgc gggtactcac
      601 agcggcgcag tgggcaccgt caacggctcg atccgcatcg atgacgacgc gaccgtcgcc
      661 agcgccggca ccgtgaacgg ctcggtcgcg ctcggggcgc acgcgaccgc cgacagcgtc
      721 ggcaccgtca acggctcgat taccctcggc gagggagccc atgtagcacg cgatgtcagc
      781 acggtgaacg gctcgatgac cttgaagagc ggcgccgagg tcagcggtcc gctgtcgaac
      841 gtgaacggtc ggatcgcgct caccgccgcc cacgtcgccg gcgggctgca cacggtggac
      901 ggggacatcg acgtcggcgg tggctcgcac gtcgagggcg gcatcctggt caagcacggc
      961 ggcggcggtt ggttcaactt cagcacgcac aagccgcgca tcgtgatcgg cccgggcgcg
     1021 gtggtgcagg gcgatctgcg ctttgagcgt gcggtcgagc tctatgtgag cgattctgcg
     1081 acgaccggcc cgatcacggg cgcgacggcc atccgcttct caggtgcgag cccgcagggc
     1141 tgacgcctca ggatcagcgc cccgcggcct tcgcgcgcag cagcgcgagc ggattcggct
     1201 gcggctcggc gatcttcacc agagtgttgc ggtcgcggtg caggaagggc tcctcgcgcc
     1261 cacggacctt cagcatagtg tcgagcacgt agctccagct gccgtcgggg ttgaagacga
     1321 cctcgatgcg ataggagtcg gtgcggaagg cgtattcgag gaacgtggtg gagcagattc
     1381 cgtactcggt ctggccgcgc tgcgcggtga gcacgagctc cgtggcatcc gccgtcgcat
     1441 gcccggcggc gatccctacc tggccgcgcg ggatgctgag cgtctgcagc accagcccgg
     1501 tcgccggctc ccacagccag taaccggtct ggtcgtgaaa ggtcgcatcc tcgtcggagg
     1561 ccaggatgcg ggtgtgatag cgcagcccgt aaaagagctg cgggccgttc gactgggcgt
     1621 cgatcggctc gagcacgatg cgctcgctgt attcgcggcg ctgtggtccc tcgggcttcg
     1681 gattgacgtc gatgcctgcg cggctctccc aggtgccagc cagccggcgg agcggcccga
     1741 ggttcgcgag agtgtcgggg tcggcttcct gctcggtgta gatgtcttcg gggaacggac
     1801 tcatgggaat gctccctgat gctcgcgggc agtctaatcg atatcgaagc agcgtagact
     1861 tcagccgcac gggcgggcgg gcccgaaccg ccgagaaagg ggtgatctga tgagtccgga
     1921 gctcaggtat ctggcgctgg tcgcgacgct gaccggcctg ttatgggtgc cgtacattct
     1981 caacgagatc gctgtgcgcg gccttttgga cgcagtcggg tatcctgcga accccaagcc
     2041 gctcgcgccc tgggcgcagc gcctcaaggg tgcgcactac aacgcggtcg agaacctggt
     2101 ggtattcgcc gcgctggtgt tggccgcggt cgccgccggt gtccatcacc ccgcggtcgc
     2161 gaccgccgcc gccatctatt tctgggcgcg gctcgtgcac gcggtggcct acacgctggc
     2221 ggtaccctgg gtgcggacgc tctcgttcct ggtcggtttc gcgatgcaga tgtgggtcgc
     2281 tgcggtcatt ctgctttagt caactggccg aagtacgcac agtccccgga gaccgggcac
     2341 tcgccgcact tcggctgcgc gcgtttgcac aactggcgcg cgtgtcgctg cagcagcagg
     2401 taggcgcgag cgcgcgcact gagcgttgcg cgcacctgcg cctcgatgag ctgctgcgcc
     2461 cgcgcgtagg tcgccgtgta cttccctgcc tcgcgtccgg atgcgaggcg caccaggacg
     2521 tggggcgagc tcgacggcac ggcggccacg ggcgccaggg cgccgaagag caggatgcga
     2581 tcggcgccgg gtgcgccgat acccggaaat ctccgcagca atttgcgcgc ctcggcctcg
     2641 g
//
LOCUS       DATLKL010000612         3256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_199053, whole genome shotgun sequence.
ACCESSION   DATLKL010000612 DATLKL010000000
VERSION     DATLKL010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199053"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VM713_08225"
     CDS             complement(<1..911)
                     /locus_tag="VM713_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84286.1"
                     /translation="MAPTPAVVLGGTGYVAGELLRLIAGHPNLELLAVTSEGSPGEPV
                     AEAFPHLASAYPETHFRSLGEVTKLLAARPAVAVFSAAPHGVSAGLIEAVLAATARAQ
                     VSARVVDISADFRYPSAQAYESVYGHAHPAAGLLRDFTCALPEHLEELSTPHVGHPGC
                     FATAVLLAAVPLLALGLVEPTLFASGVTGSTGSGRKPVEGTHHPLRHGDLYGYNALAH
                     RHAPEIVACARAATGVEAELAFVPHSGPFARGIYVTVQARLTRALDSSEARGALREYY
                     ARAPFVRVSGRAPHVKEVATSNFAHLSA"
     gene            complement(929..1999)
                     /locus_tag="VM713_08230"
     CDS             complement(929..1999)
                     /locus_tag="VM713_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84287.1"
                     /translation="MSMRSVIVDKLASVTQACGLSHEVRISAEIPAEEGVVIVVEVLN
                     NKSTYNTLELVSGRMAKLGKGDIVAGALGHRQALFGYSGHVPESVQPGDIIQMLNVGG
                     VLGVCDSINPEKGRPFDCRVLGVALHFPYLGERIGVPARAGYKRLDANAALETRGVPV
                     VALAGTCMEAGKTAAACAIIARMRHRGLTVDAFKATGVSLRRDILAMEDAGARRCAIF
                     TDLGVVTTTRANGPALTRTMLTELAAAKPDVLVFELGDGILGTYGVDAILECPDIRTA
                     LTGVVLSANDPVAAWGGVKLLRERFGIEPCVVTGPCTDNQVGVEIITQQMGVPACNAI
                     SSAAALGDRVIAALGLTAAAAA"
     gene            complement(2004..2723)
                     /locus_tag="VM713_08235"
     CDS             complement(2004..2723)
                     /locus_tag="VM713_08235"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84288.1"
                     /translation="MPPSGAAYSINRGLWGVTIGGQETLTSSGSIPDDAWLLTREAFT
                     RARAPARHVLGFERGRPVALDGRSGAPVEIIERLEALAAPFGIGRGIHLGDTIIGTKG
                     RVAFEAPAAEVLLTAHRELEKLTLTGRQQRIKELLAAPYGDLVHEGQLLDPVCRDIEA
                     LLASSQQRVTGEVRFKLQTGNLFIEGVESPYSLMAASRGVYGEAAGEWSAADALGFSR
                     IAALPGAFYARAGARAAAGEK"
     gene            complement(2736..3254)
                     /locus_tag="VM713_08240"
                     /pseudo
     CDS             complement(2736..3254)
                     /locus_tag="VM713_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012966.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
BASE COUNT          470 a         1132 c         1204 g          450 t
ORIGIN      
        1 gccgacaggt gcgcgaagtt gctggtcgcc acctctttga cgtgcggcgc gcgcccgctc
       61 acccgcacga acggcgcgcg cgcgtagtac tcgcgcagcg cgccgcgcgc ttcgctgctg
      121 tcgagcgcgc gcgtcaggcg cgcctgcacc gtgacgtaga tgccgcgcgc gaaggggccc
      181 gaatgcggta cgaaggcgag ctcggcctcg acgccagtcg cggcgcgcgc gcaggcgaca
      241 atctccggcg catgccggtg ggcgagcgca ttgtagccgt agagatcgcc gtgccgcagc
      301 ggatggtgcg taccctcgac gggcttgcgg cccgacccgg tgctgccggt gacgccgctc
      361 gcgaacaagg tcggctcgac gagcccgagt gccagcagcg gcaccgcggc gagcagcacc
      421 gcggtggcga agcagcctgg atggccgaca tgaggcgtgc tgagctcctc taggtgctcg
      481 ggcagcgcgc aggtgaaatc tctgagaagc ccggcggcgg ggtgcgcgtg gccgtagacg
      541 ctctcgtagg cctgtgccga ggggtagcgg aagtcggccg agatatccac gacccgcgcg
      601 ctcacctgcg cgcgcgcggt cgcggcgagc accgcctcga tgaggcccgc cgacacgccg
      661 tggggcgcgg cggagaacac cgccaccgcc ggcctcgcgg cgagcagctt cgtcacctca
      721 ccgagcgagc ggaagtgcgt ctccgggtag gcggaggcga ggtgcggaaa cgcctccgcc
      781 accggctcgc cgggcgagcc ctcggaggtc acggcgagga gctcgaggtt gggatggccg
      841 gcgatgaggc gcagcagctc tccggcgacg tagccggtgc cgccgagtac caccgccgga
      901 gtaggagcca cgctggggct gcccgcgctc acgcggccgc cgctgcggtg agtccgagcg
      961 ccgcgatcac gcggtcgccg agcgcggcgg cggagctgat cgcattgcag gccggcacgc
     1021 ccatctgctg cgtgatgatc tcgaccccca cctggttgtc ggtgcagggt ccggtcacga
     1081 cgcacggctc gatcccgaag cgctcgcgca gcagcttcac cccaccccag gccgccaccg
     1141 gatcgttggc cgacagtacg acgcccgtga gcgcggtgcg gatgtcgggg cactcgagga
     1201 tcgcatccac gccgtaggtc ccgagaatgc cgtcaccgag ctcgaacacc agcacgtcgg
     1261 gcttggctgc ggcgagctcg gtcagcatgg tgcgggtgag ggccggaccg ttggcgcgcg
     1321 tcgtggtgac cacgcctaag tcggtgaaga tcgcgcagcg ccgcgcgccg gcgtcctcca
     1381 tcgcgaggat gtcgcgcctc agggacacac cggtcgcctt gaaggcatcg accgtgagcc
     1441 cgcggtggcg catgcgggcg atgatcgcgc aggcggcggc cgtcttgccc gcctccatgc
     1501 aggtgccggc gagcgccacg accggcacgc cgcgcgtctc gagcgcggca ttcgcatcga
     1561 gccgcttgta gccggcgcgc gccggcacgc cgatgcgctc cccgaggtag ggaaagtgga
     1621 gggcgacgcc gagcacgcgg cagtcgaagg gcctgccctt ctcggggttg atcgagtcgc
     1681 acacgccgag cacgccgccg acgttgagca tctggatgat gtcgcccggc tgcacggact
     1741 cgggcacgtg tcccgagtag ccgaacagcg cctggcggtg gccgagcgcg ccggccacga
     1801 tgtctccctt gccgagcttg gccatgcggc cgctcacgag ctcgagcgtg ttgtaggtcg
     1861 acttgttgtt gaggacctcg accacgatca cgacgccttc ctccgcgggg atctcggcgg
     1921 agatgcgcac ctcgtgcgac aggccgcagg cctgggtgac ggaggcgagc ttgtcgacga
     1981 tgacgctgcg catgctcacc atgtcacttc tctcccgccg ccgcgcgcgc gccggcgcgc
     2041 gcgtagaacg ccccggggag cgcggcgatc cgcgagaagc cgagcgcatc cgcggccgac
     2101 cactcgccgg ccgcctcgcc gtagacgccg cgcgaggcgg ccatgagcga gtagggcgac
     2161 tcgactccct cgatgaagag attcccggtc tggagcttga agcgcacctc gccggtcacg
     2221 cgctgctgcg aggaggcgag cagcgcctca atgtcgcgac agaccggatc gagcagctgg
     2281 ccctcgtgca ccaggtcgcc gtagggcgca gccaggagct ccttgatgcg ctgctgccgt
     2341 ccggtgaggg tcagcttctc gagctcgcga tgcgccgtga gcagtacctc ggctgccgga
     2401 gcctcgaacg ccacccgtcc cttggtgccg atgatggtgt ccccgaggtg gatgccgcgc
     2461 ccgatgccga agggcgcggc gagcgcttcg agccgctcga tgatctcgac cggcgcgccg
     2521 ctgcgaccgt cgagcgcgac cggccggccc cgttcgaagc cgagcacgtg ccgcgccggc
     2581 gcccgcgcgc gtgtgaatgc ttcgcgggtc aggagccatg cgtcatcggg gatgctgccg
     2641 ctcgaggtga gcgtctcctg gccgccgatg gtgacgcccc agagtccccg gttgatcgag
     2701 taggcggcgc cgctcggcgg cacggggagc ccccgcgcct tgagatactc gagctcctcg
     2761 gcgcgcttga aggcccggtc gcgcaccggg gcgagcaccg cgaggtcggg cgcgagcgtg
     2821 cgcagcgcga cctcgaagcg cacctggtca ttgccggccg cggtgcaacc gtgcgccacg
     2881 gtgtcagact tgagcgcgcg cgccatgcgg gcgatcgtct gcgcctgcat cacgcgctcg
     2941 gcaccgacgc acagcgggta gagctgaccg cgccgcacgt ttcccatgat gagaaagcgc
     3001 agcacctgct cgaagtaatc ggcgcgcgca tcgatctggt gatgctcgag ggccccgagc
     3061 gcccgcgcgc gctcagcgag cgcgcgggcc gtctaggcgt cgatgccacc ggtatcgacc
     3121 gtcaccgtga tcaccgggcg gcggtaggtt tcggcaatcc attgctccag gaacgaggtg
     3181 tcgaggccgc cggagtaggc gaggacgatg ggggcggtgc ccgcctgagg ggtgctgctg
     3241 gcgcttgtcg gcatgg
//
LOCUS       DATLKL010000613         3722 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_199055, whole genome shotgun sequence.
ACCESSION   DATLKL010000613 DATLKL010000000
VERSION     DATLKL010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199055"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VM713_08245"
     CDS             <1..391
                     /locus_tag="VM713_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVH84289.1"
                     /translation="QARAAYDAAVANYRQTVLAAFQQVEDQLATLRILEQQVQVQEQT
                     VQSANLAVQLTLNQYKAGTVAYTSVITAQTIALADAQTLLNIRQSRLNASVTLIEALG
                     GGWNAASLGRSTGTGAELSAQEAARGH"
     gene            540..1043
                     /locus_tag="VM713_08250"
     CDS             540..1043
                     /locus_tag="VM713_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84290.1"
                     /translation="MRTRLSARHRRALYFFATVLWLSGVAWLIAHYALNPSDPLAGAH
                     PSEPWWLRLHGAAVIGFLVAFGALLPGHIRRGWRQGLNRGSGLTIIIVTAALTLTGYG
                     LYYVVDDAWRAWGSLVHWLVGLASGVVLTMHVWLGKRGVARARLRRATRNPHHRLPAR
                     GPGPNTS"
     gene            complement(976..1917)
                     /locus_tag="VM713_08255"
     CDS             complement(976..1917)
                     /locus_tag="VM713_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HVH84291.1"
                     /translation="MEFAGSWPSVLSPSPSGDAYAAAGARIRRARPLLGTRVEIGVAG
                     PVPVAELHQAVDAAFAAIERIERLMSYHDPHSELSRINREGAVRAQRVHPDTCAVVRA
                     ALRFAALGGGAFDPCVAPCLEEWGLLPRHASARADPAASWRDIEILDGDQVRFWRALR
                     LDLGGIAKGYAVDQAVRVLKAAGVGSILVNAGGDLRVAGEGFEAIGLRSPRTPWLAAH
                     TLTLRDTALATSSACYSRRRTSAGVEVSALLDPMSRAAHLGADSVSVRAGDCMTADAL
                     TKIVLFADRQTGERALRTCSARALVLGADGADCASRA"
     gene            complement(1865..2392)
                     /locus_tag="VM713_08260"
     CDS             complement(1865..2392)
                     /locus_tag="VM713_08260"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HVH84292.1"
                     /translation="MFTPSRIVFLPVAALAGAQPAQAMVYLTIGAAQGLMFPGQTLAP
                     VPLTLSAEEIATVERETGTRYVAGALRAWRASDGFFLVDAVIGKHDLISYAVALTADG
                     KIRQVEILEYREAYGGEVRNAAWRGQFIGRSHGDPVQLGRDIRNISGATLSCQHVTDG
                     IRRLLAVCAIALAKR"
     gene            complement(2409..>3722)
                     /locus_tag="VM713_08265"
     CDS             complement(2409..>3722)
                     /locus_tag="VM713_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84293.1"
                     /translation="DPVAGDVEQRLEQEIRALQQQLESTQQQLARMQAELQQLKAQKE
                     AVAAQQQQAALPGAGAASAAAGISPDLSLWGYGEIYYDRPVHIPELAEADLARAVFGI
                     GYRFDDRTLFNSEFEVEHAVSSASDRGEFEVEQFYVDHQLAAWAAVKGGLILMPFGLL
                     NEHHEPTQFYGVKRNHVEKLIIPTTWREGGVGFHGDTPGGLVWDAGVTTGINLKDWNF
                     TPAAPFYRTSLDLEAADIAPMQATHQELELADAQHLAQYAALNYSGVPGLLAGAAVFS
                     GKAAVPAGFPDVRATLWEVHGRWTAGRADLSALYARGTFSDTAPFNLANLGASNPLPA
                     EFLGYYLQGAYTVWEQGSYRLSPFIRWERYDMGAAYQGIAPGYTTVPPGPAPDGRPWP
                     QPRDRVWTAGANFYLTPHVVLKADYQRFAINTDFSSIDLGLGVNF"
BASE COUNT          602 a         1260 c         1240 g          620 t
ORIGIN      
        1 ccaggcgcgc gccgcttatg acgccgcggt cgccaactac cgccagaccg tgcttgccgc
       61 ctttcagcag gtcgaggacc agctggcgac gctgcgaatc ctcgagcagc aggttcaggt
      121 gcaggagcag acggtgcagt ccgcgaacct cgcggtccag ctgacactca accagtacaa
      181 ggcgggaacc gtcgcataca cctcggtgat cactgcgcag accattgcgc tcgcggacgc
      241 gcagacgctg ctcaacatcc gccagagccg tttgaacgcg agcgttactc tcatcgaggc
      301 gctcggcggc ggctggaacg cggcttcgct cggccgatcg accggcaccg gcgcggagct
      361 ttccgcacag gaagccgccc ggggccactg accctgggcg tatcgctcct tcagtcaagc
      421 cgatttcagg ttggcccgtt gacggtggct cgacggcttt ctatgattta aatgcgaatg
      481 attctcatta gaaagtccag ccggccggat ctccgcggat gaggcgccac gagcacggag
      541 tgcgcaccag gctcagcgcg cgccatcggc gcgcgctcta cttctttgcg acagtcctgt
      601 ggctctcggg agtcgcatgg ttgatcgctc actatgcgct gaacccgagc gatcccctcg
      661 cgggtgcgca tccttccgag ccctggtggc tccggctgca cggggccgcc gtcattggct
      721 ttctcgtggc tttcggcgcg ctgctgccgg ggcacattcg acgcggctgg cggcagggac
      781 tgaaccgcgg cagtggtctc acgatcatca tcgtcaccgc ggccctgacg ctcacaggct
      841 acggactcta ttacgtggtc gacgatgcgt ggcgcgcgtg gggcagcctg gttcactggc
      901 tggtcgggct ggcgagcggc gttgtgctca cgatgcacgt gtggctcggg aaacgcggcg
      961 tcgcgcgcgc ccgcctcagg cgcgcgacgc gcaatccgca ccatcggctc ccagcacgag
     1021 ggcccgggcc gaacacgtcc tgagcgcccg ttccccggtc tgtcggtcgg cgaacagcac
     1081 gatcttggtg agtgcgtccg ccgtcatgca gtcgccagcg cgcacgctga cgctgtcggc
     1141 tccgagatgg gcagcgcggc tcatgggatc gagcagcgcc gagacctcga cgccggcgct
     1201 tgtccgccgc cgcgaatagc aggccgacga ggtggctagt gccgtgtcgc gcagcgtgag
     1261 ggtgtgtgcc gcgagccacg gcgtccgggg actgcgcagc ccgatcgcct cgaatccctc
     1321 cccggcgacg cgcagatcac cgcctgcgtt gaccaggatg ctgccgacgc ccgcggcttt
     1381 gagcacgcgc accgcctgat ccaccgcgta gcccttggcg atgccgccga gatcgagccg
     1441 cagcgctcgc cagaagcgca cctggtcccc atccagaatc tctatatcgc gccaggaggc
     1501 ggcgggatct gcgcgggctg acgcatgtcg tggcaacaat ccccactcct cgaggcacgg
     1561 agcgacgcag ggatcgaacg ccccgccgcc gagcgccgcg aagcgcagcg cggcgcgcac
     1621 gaccgcgcag gtgtcggggt gcacgcgctg cgcgcgcacc gctccctccc ggttgattcg
     1681 cgagagctcg ctgtgaggat cgtgatagct catcagccgc tcgatgcgct cgatcgccgc
     1741 aaacgccgcg tcgaccgcct gatgaagctc agcgaccgga actggtccgg cgacaccgat
     1801 ttcgacccgc gtgccgagca gcggccgcgc gcggcggatc cgcgcgccgg cggcggcgta
     1861 agcgtcaccg cttggcgagg gcgatagcac agacggccag gagccggcga attccatcgg
     1921 tgacgtgttg gcaggagaga gtcgcgccgc tgatgttgcg gatgtcgcga ccgagctgca
     1981 ccggatcacc gtgcgagcgg ccgatgaact gaccgcgcca ggctgcatta cgcacttcgc
     2041 caccgtaggc ttcgcgatac tcgaggatct cgacctggcg gatcttgccg tcggccgtga
     2101 gggcgaccgc gtagctgatg aggtcgtgct tgccgatcac cgcatcaacc aggaaaaaac
     2161 cgtcgctggc tcgccacgcc cgcagggccc cggcgacgta gcgtgtgccg gtctcgcgct
     2221 cgacggtggc aatctcctcc gcgctcagcg tcagcggcac gggcgccaga gtctgtccgg
     2281 gaaacatgag tccctgggcc gcaccgatgg tgagatagac catcgcctgc gcgggttgcg
     2341 caccggcaag cgccgccacc ggcaggaaca cgatgcgcga gggggtgaac atcttcagaa
     2401 ccgtcgcgtt agaaattcac accgaggccg agatcgatcg agctgaagtc ggtattgatg
     2461 gcgaaccgct ggtaatcggc cttgaggacg acgtgcgggg tcaggtagaa gttcgcgccg
     2521 gcggtccata cccggtcgcg cggttgtggc cacggcctgc cgtccggagc cggccccggg
     2581 ggcacggtgg tgtagcccgg cgcgataccc tggtaagcgg ctcccatgtc gtagcgctcc
     2641 cagcgaatga atggactcag gcgatagctg ccctgctccc acaccgtata ggcaccctgc
     2701 agatagtagc caagaaactc cgccggtaat gggttgctcg ctcccaggtt ggcgagattg
     2761 aacggcgcgg tgtccgagaa ggtaccgcga gcgtacagcg ccgagagatc cgctctcccc
     2821 gcggtccatc gcccgtgtac ttcccatagc gtggcgcgca cgtccgggaa tcctgccggc
     2881 acggccgcct tgccgctgaa cacggcggcg cccgccagca gccccggcac accgctgtag
     2941 ttgagcgctg cgtactgagc caggtgctgc gcatcggcga gctccagctc ctggtgggtc
     3001 gcctgcatgg gcgcgatgtc cgcggcctcg agatcgagcg aggtgcggta aaacggcgcc
     3061 gctggcgtga agttccagtc cttgagattg attccggtcg tcacgcccgc atcccagacg
     3121 aggccgcccg gcgtgtcgcc atgaaaaccc actccgcctt cacgccaggt ggtgggaatg
     3181 atcagcttct cgacgtggtt acgcttcacg ccatagaact gggtcggctc gtgatgctcg
     3241 ttcaagagtc cgaacggcat caggatgagg ccacccttga ctgcggccca cgccgcgagc
     3301 tggtgatcga cgtagaactg ctcgacctcg aattcgccgc ggtcgcttgc ggagctcacg
     3361 gcgtgctcga cttcgaactc cgaattgaag agcgtgcggt catcgaaccg gtacccgatg
     3421 ccgaacacgg cgcgcgcgag gtccgcttca gcgagctcag gtatgtgcac ggggcggtcg
     3481 tagtagatct cgccgtagcc ccagaggctc agatcgggcg agatccctgc cgcagcggac
     3541 gctgcccccg ctcccggcaa tgccgcttgc tgttgttgcg ccgcgaccgc ttctttctgt
     3601 gccttgagct gctgcagctc ggcttgcatc ctcgccagct gctgctgcgt ggactccagc
     3661 tgctgctgca gtgcgcggat ctcctgctcg agtcgctgct cgacatcgcc cgcgacaggg
     3721 tc
//
LOCUS       DATLKL010000614         1790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_18584, whole genome shotgun sequence.
ACCESSION   DATLKL010000614 DATLKL010000000
VERSION     DATLKL010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18584"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..692
                     /locus_tag="VM713_08270"
     CDS             192..692
                     /locus_tag="VM713_08270"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HVH84294.1"
                     /translation="MGRLHITCLLSVAALATLAACKTEPVDRAGTALPERARYMPYVG
                     APITSFYWMMRFDSWEALSDSELVMFINPDTAYYLKISTPCGPAGLGFAHVIGLTSSV
                     GGAVIAHLNSVRTEGLNCQISEIRTVDYTRMREDGRALRVASAPSTSEPAATPAGAPI
                     AAIPPR"
     gene            complement(710..>1790)
                     /locus_tag="VM713_08275"
     CDS             complement(710..>1790)
                     /locus_tag="VM713_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVH84295.1"
                     /translation="AIFRRLYGEDRALAVRRDAWGFALGLIATAALYGAYAWIAVCTV
                     RSLITLGQMTMYLALFRQGQSAVSAMLSAVGGMYEDNLYLSTLYEYLETPVAALAGSA
                     PRGPHPEDGIRFEDVSFSYPGADAPALEHVTLHLQPGASLALVGENGSGKTTLIKLLT
                     RLYAPTAGRILLDGRDLGEWDEAALRERIGVIFQDFARYQMLVGENVGAGDERYFEDE
                     ARWRTAADKGRASEFIATLPAGYHTQLGKWFRDGRELSGGQWQKIALSRAFMRTRADI
                     LVLDEPTASMDAQAEAEVFEHFRQLARDRITILISHRFSTVRMADQIAVLAGGRIVER
                     GTHEELMRLDGRYAHLFTLQARGYR"
BASE COUNT          305 a          611 c          597 g          277 t
ORIGIN      
        1 acccggccgt aatatgtagc cccgcgcctg cagagtgaac aggtgccgta gcggccgtcg
       61 aagcgcatca gttcctgccg agctgaaggc cacgctccaa gtgtccgcag cgggcgacag
      121 gctcccgccg caggctccca cccactagaa tcaagacttg cgcataagtg cccaccctgc
      181 gtgagaattg catgggcaga ctccacatca cctgcttgct ctcagtcgcg gcgctcgcga
      241 cgctcgcggc gtgcaagacg gagccggtag acagagcggg gaccgccctg ccggagcggg
      301 cgcgctacat gccttacgtc ggtgcgccca tcacgagctt ctactggatg atgcgcttcg
      361 acagttggga ggctctgagt gacagcgagc tcgtgatgtt catcaatccc gacacggcct
      421 attacctcaa gatatcgaca ccgtgcggcc cggcggggtt gggtttcgcc cacgtcattg
      481 ggcttacctc ctccgtcggc ggtgcggtga tcgcgcacct gaattcggtg cggaccgagg
      541 ggttgaactg tcagatctct gagatccgca ccgtcgacta cacgcgcatg cgcgaagatg
      601 ggcgcgcgct gagagtggcg agcgcaccgt ccacgtctga gccagcagca acgcccgctg
      661 gggcaccgat cgcggccata ccccccaggt agctggaggc ggcggcccct cagcggtagc
      721 cccgcgcctg cagcgtgaac aggtgcgcgt agcggccgtc gaggcgcatc agctcctcgt
      781 gcgtgccgcg ctcgacgatg cggccgccgg cgagcacggc gatctgatcg gccatgcgca
      841 ccgtcgagaa gcggtgcgag atgagaatcg tgatgcggtc gcgtgcgagc tggcgaaagt
      901 gctcgaacac ctcggcctcc gcctgggcat ccatcgacgc ggtcggctca tcaagcacca
      961 ggatgtcggc gcgcgtgcgc atgaaggcgc gcgatagggc gattttctgc cactggccgc
     1021 ccgagagctc gcgtccgtcg cggaaccact tgccgagctg agtgtggtag ccggccggca
     1081 acgtcgcgat gaattcgctc gcccggccct tgtcggccgc ggtgcgccag cgcgcctcgt
     1141 cctcgaagta gcgctcatcg cccgcgccga cgttctcgcc caccagcatc tggtagcggg
     1201 cgaaatcctg gaagatgacg ccgatgcgct cgcgcagcgc cgcctcgtcc cactcgccga
     1261 ggtcgcggcc atcgagaagg atgcgcccgg cggtcggcgc atacaggcgc gtcaggagct
     1321 tgatgagcgt ggtcttgccc gagccgttct cgccgacgag cgcgagactc gcaccgggct
     1381 gcaggtgcaa cgtgacatgc tcgagcgccg gcgcatcggc cccggggtag ctgaagctca
     1441 cgtcctcgaa gcgaatgcca tcctcgggat ggggaccgcg cggcgcgctg ccggccagcg
     1501 ccgctaccgg cgtctccaga tactcgtaca gcgtcgagag atagaggttg tcctcgtaca
     1561 tgccgccgac cgccgacagc atcgcggaca ccgctgactg gccctggcga aacagcgcca
     1621 gatacatcgt catctggccg agcgtgatga gcgagcgcac ggtgcacacc gcgatccagg
     1681 cgtacgcccc gtagagcgcc gcggtggcga tcagtccgag tgcgaagccc cacgcgtcgc
     1741 gccgcacggc gagtgcccga tcctccccat acagccggcg gaagatggcg
//
LOCUS       DATLKL010000615         1997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_142970, whole genome shotgun sequence.
ACCESSION   DATLKL010000615 DATLKL010000000
VERSION     DATLKL010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142970"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VM713_08280"
     CDS             <1..444
                     /locus_tag="VM713_08280"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HVH84296.1"
                     /translation="NEEGVLRFLLAAAAMGMLYKRNASISGAEMGCQGEVGVACSMAA
                     AGLVAALQGTNEQVENAAEIGMEHNLGLTCDPVAGLVQIPCIERNAMGAVKAINAARL
                     AMRGDGTHKVSLDQVIATMRQTGADMSTIYKETSQGGLAVNVPEC"
     gene            complement(473..985)
                     /locus_tag="VM713_08285"
     CDS             complement(473..985)
                     /locus_tag="VM713_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HVH84297.1"
                     /translation="MKRLTVMRHADARWKNPGTDDFARPLNRRGLAGAESMARRPLEL
                     ELVPDRLLASTARRAEQTADIVARELSLSVRHVQREESLYLASAADMLRLIQETGPRI
                     AHLMVIGHNPGASHLIHLLVPRGGISTLTTAGLCSIVFEAADWRAIGPEAVKEVQHHA
                     PPSRLWGLIR"
     gene            complement(996..1310)
                     /locus_tag="VM713_08290"
     CDS             complement(996..1310)
                     /locus_tag="VM713_08290"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HVH84298.1"
                     /translation="MSGWIDIGPVSELGEGVPLSAEVDGVPVVVVRCGSDLYAVEDRC
                     THDGESFAGAEVESCELICPRHGSRFDLRTGDALTPPAYEPVRTYTVREQNGRILVEQ
                     PA"
     gene            complement(1307..>1997)
                     /gene="sufT"
                     /locus_tag="VM713_08295"
     CDS             complement(1307..>1997)
                     /gene="sufT"
                     /locus_tag="VM713_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HVH84299.1"
                     /translation="DAALGKLAALSGVREFPARVKCATLSWHTLNAAMERGAATVSTE
                     WRVRRHENEPFVVSREVRAVIVPAGQEVKLKPGQAGYITQALGGSFTVYLEGNLFRIA
                     GEDADAIGQEAVKPPELPPNATAEDVRKLAWEQMRSCYDPEIPINIVDLGLVYECDVK
                     ENDDGSRTVEAQITLTAPGCGMGDILVDDVRDKLQRIPTVREAHVQLTFDPPWNQSMM
                     SEAARLQTGMF"
BASE COUNT          302 a          674 c          707 g          314 t
ORIGIN      
        1 aacgaggagg gtgtgctgcg gttcctcctg gcggcggccg ccatggggat gctctacaag
       61 cgcaatgcct ccatctcagg cgccgagatg ggctgtcagg gcgaggtggg ggtcgcctgc
      121 tcgatggcgg cggctggact ggtcgcggcg ctgcagggca ccaacgaaca ggtcgagaac
      181 gccgccgaga tcggcatgga gcacaacctc gggctcacct gcgatccggt cgcgggactg
      241 gtgcagattc cgtgtatcga gcgcaatgcc atgggcgcgg tcaaggcgat caacgcggcg
      301 cggctggcga tgcgcggcga cggcacgcat aaggtgtcgt tggaccaggt gatcgccacg
      361 atgcgccaga cgggcgccga catgtcgacg atctacaagg agacctcgca gggcgggctc
      421 gcggtcaacg tcccggagtg ctgacccgcc cggcggcaaa ccggcctgcc ggttaacgaa
      481 tcaaccccca cagccgcgac ggcggcgcgt ggtgctgcac ttccttcacc gcctcggggc
      541 cgatcgcgcg ccagtcggcg gcttcgaaca cgatcgagca cagcccggcg gtggtgagcg
      601 tgctgatccc gccgcgcggc accagcaggt ggatgagatg ggaggcgccg gggttgtgcc
      661 cgatcaccat gaggtgtgcg atacgcgggc cggtctcctg gatgaggcgc agcatgtcgg
      721 cggcgctcgc gagatatagc gactcctcgc gctgcacgtg ccgtaccgac agcgacagct
      781 cgcgcgccac gatgtcggcg gtctgctccg cacgccgcgc ggtgctggcg agcaaccgat
      841 cgggaacgag ctcgagctcg aggggccgtc tcgccatgga ctccgcgccc gccaggccgc
      901 ggcggttgag ggggcgggcg aagtcatcgg tccccgggtt cttccagcgg gcatccgcat
      961 gacgcatgac ggtcaggcgt ttcatgcgcg gcgcctcagg cgggctgctc aaccaggatc
     1021 cggccgttct gctcgcgcac cgtgtaggtg cgcaccggct cgtaggcggg cggggtgagg
     1081 gcatcgccgg tgcgcaggtc gaagcgcgag ccgtggcggg ggcagatgag ctcgcaggac
     1141 tccacctcgg cgcccgcgaa ggactcgccg tcgtgcgtgc agcggtcttc cacggcgtac
     1201 aggtcgctcc cgcagcgcac gactaccacc ggcacgccgt cgacctccgc ggacaggggt
     1261 acgccctccc cgagctcgct caccggcccg atgtcgatcc agccgctcaa aacatccccg
     1321 tctgcaaccg cgcggcctcc gacatcatcg actggttcca gggcgggtcg aaggtcagct
     1381 gcacatgcgc ctcgcgcacc gtcgggatcc gctgcagctt gtcgcgcaca tcgtccacca
     1441 ggatgtcccc catgccgcag ccaggcgccg tgagcgtgat ctgcgcctcg acggtgcggc
     1501 taccgtcgtc gttttccttc acatcgcatt catacacgag tccgagatcc acgatgttga
     1561 tggggatctc agggtcgtaa cagctgcgca tctgttccca ggccagcttg cgcacgtcct
     1621 cggcggtggc gttcggcggg agctccggcg gcttgaccgc ttcctggccg atggcgtcgg
     1681 catcctcgcc ggcgatgcgg aacaggttcc cctcgaggta gacggtgaag ctgcccccga
     1741 gcgcctgcgt aatgtagcct gcctgcccgg gcttgagctt gacctcctgg ccggcaggaa
     1801 cgatcaccgc gcgcacttcg cggctcacga cgaagggctc gttctcgtgg cggcgcacgc
     1861 gccactccgt cgacacggtg gcggcgccgc gttccattgc ggcattgagc gtgtgccagc
     1921 ttaaggtggc gcacttcacc cgcgcgggga attcgcgtac gccggagagg gcggcgagct
     1981 tgccgagcgc ggcgtcg
//
LOCUS       DATLKL010000616         2716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_41328, whole genome shotgun sequence.
ACCESSION   DATLKL010000616 DATLKL010000000
VERSION     DATLKL010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41328"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /gene="hpnA"
                     /locus_tag="VM713_08300"
     CDS             complement(<1..845)
                     /gene="hpnA"
                     /locus_tag="VM713_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HVH84300.1"
                     /translation="MRALVTGATGFVGSAVARALLDAGARVRALVRPGADRANLRMLP
                     IETAIGDLGDSASLVEALCGCEVLFHVAADYRLGALDPERLYWTNVAGTRNVLNAARR
                     AGVARIVYTSSVATVGLPADGSPGQEDTPVELADMIGHYKRSKFLAEQVVRDFIRDGL
                     PVVIVNPSTPIGPGDLKPTPTGRVVVDAACGRTPAYVDTGLNVVHVDDVAIGHLRACE
                     RGRVGERYILGGENMTLREILGQVCRLSGRAPPRIRLPHAALLPIALVSETRARACGR
                     SARVT"
     gene            complement(842..1579)
                     /locus_tag="VM713_08305"
     CDS             complement(842..1579)
                     /locus_tag="VM713_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84301.1"
                     /translation="MSGVGIVAALRAESRLLGPVRARRGSCAVLADGTLLVVSGMGLA
                     AAGEGARRLVPAGARALVSFGMAGGLDPRLVAGSIVLPSEVVSSDGAHFATTGHWREH
                     VGAAVGARHPVCFGALLSCRQAIGSVADKAEVFRNTAAAAVDMESAAVAEVATSERLP
                     FLAVRAIVDTASDSLPPALVEVIGPGGAARIGRVLALLARRPADLGLLFQLLRRYRAA
                     RRALATVARCGALALHAGDPRSEGVGT"
     gene            complement(1576..>2716)
                     /gene="shc"
                     /locus_tag="VM713_08310"
     CDS             complement(1576..>2716)
                     /gene="shc"
                     /locus_tag="VM713_08310"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HVH84302.1"
                     /translation="EDGLGAIFPAMVNALEALAVLGYPREDPRRAAATRALQRLLIVD
                     VSSAYCQPCVSPVWDTALAALAMQEAGGPRALAAARRALAWLEPLQLLGAPGDWQAAR
                     ATLRGGGWPFQRANDYYPDLDDTAAVTWAMERALEGGRYAYAEERALDWLAGMQSSNG
                     GFASFDVDNTRYYLNAIPFADHGALLDPPTSDVTARVVTALAVSGRAQDAAALERAVA
                     FLRAEQEADGSWFGRWGTNYIYGTWSVLTAFSHAGIAPDDPAVRRAAAFLAARQNPDG
                     GWGESNDSYWRERGGTPAASTPHQSAWAILGLLAAGEAATEAVQRGVRYLLRAQRADG
                     LWSDPAFTAPGFPRVFYLRYHGYCAYFPLWALAAYRSHSRPGTLH"
BASE COUNT          455 a          993 c          925 g          343 t
ORIGIN      
        1 atggtgaccc gggcgctgcg gccgcaggcg cgcgcgcgcg tctccgacac cagggcgatt
       61 ggcaggagtg ccgcatgcgg caggcggatg cgcggcggtg cccgccccga cagccggcag
      121 acctgtccca gaatctcgcg cagcgtcatg ttctcgccgc cgagaatgta gcgttcgccc
      181 acgcgccctc gctcacaggc tcgcaggtgg ccgatggcga catcatccac gtgtaccacg
      241 ttgagtccgg tgtcgacgta ggcgggcgtg cgcccgcagg ccgcatccac gacgacccgg
      301 ccggtcgggg tgggcttgag gtcgcccggc ccgatcggtg tggacgggtt gacaatgacg
      361 acgggaagac cgtcgcggat gaaatcgcgc accacctgct ccgccagaaa cttcgagcgc
      421 ttgtagtgac cgatcatgtc cgcgagctcg accggtgtgt cctcctggcc cggtgagccg
      481 tccgccggca gcccgaccgt ggcgacgctg ctggtgtaga cgatgcgcgc gacgccagcc
      541 cgccgtgcgg cattgagcac gttgcgcgta cccgcgacgt tggtccagta gagccgctcc
      601 gggtccaggg cgcccaggcg gtaatcggca gccacgtgaa acaacacctc gcaaccgcac
      661 aaggcttcaa cgagcgaagc gctatcgcct agatcgccga tcgctgtctc gatcggcagc
      721 atgcgcagat tggcgcgatc ggcgccgggc cgtaccaggg cgcgcacgcg ggcgccggca
      781 tcgagcagcg cccgggcaac cgccgagccc acgaacccag tcgcccccgt aacgagagcc
      841 ctcatgtccc gacgccctcg ctgcgggggt cgcccgcgtg cagagccagt gctccgcacc
      901 gcgcaaccgt cgcgagcgcc cggcgcgccg cacggtagcg gcgcagcagc tgaaacagca
      961 gcccgagatc cgcgggccgc cgggcgagca atgcgagcac gcgtccgata cgcgcggcgc
     1021 ccccgggacc tatgacttcg acgagggcag gcgggagact gtccgaagcg gtatcgacga
     1081 tcgcgcgcac cgcaagaaag ggcagccgct cactcgttgc cacctccgca acggccgcgc
     1141 tctccatgtc gaccgcggcc gcggccgtgt tgcggaacac ttccgccttg tcggcaaccg
     1201 aaccgatcgc ctgacggcag ctcaagagtg ctccgaaaca cactgggtgg cgcgcgccga
     1261 cagcggcgcc cacgtgctca cgccagtgtc ctgtggtagc aaagtgcgca ccgtcgctcg
     1321 agacgacctc gctcggcaac acgatgcttc cggcgaccag gcgcggatcg agcccgccgg
     1381 ccatgccgaa gctgacgagc gctcgagcac ccgccggaac cagccgccgc gccccctcgc
     1441 ccgccgcagc gagacccatg ccactcacga cgagcagtgt gccgtcggcc agcaccgcgc
     1501 acgatccgcg ccgcgcgcgc accggcccca gcaggcgcga ctcggcgcgc agcgctgcca
     1561 cgattccaac accgctcaat ggagcgtacc ggggcgcgaa tgcgagcggt aggctgccag
     1621 cgcccacaaa gggaaatacg cgcaataccc gtgatagcgc agatagaaga cccgcggaaa
     1681 tccgggagcc gtgaaggcgg gatcgctcca cagcccatcc gcgcgctgcg cacgcaacag
     1741 atagcggacc ccgcgttgca ccgcctccgt cgccgcctcc cctgctgcga gcagcccaag
     1801 gatcgcccaa gcgctctggt gcggcgtgct cgcggccggc gtcccgccgc gctcgcgcca
     1861 atagctgtcg ttgctctccc cccagccgcc gtcggggttc tgccgcgccg cgagaaaggc
     1921 agccgcgcgc cgcaccgccg ggtcatcggg cgcgatgccg gcgtgggaga atgcagtgag
     1981 gaccgaccag gtgccgtaga tgtagttcgt cccccagcgc ccgaaccagg agccgtcggc
     2041 ctcctgctcg gcgcgcagga aggcgacggc gcgctcgagc gccgccgcat cctgggcacg
     2101 tcccgagacc gcaagcgctg taaccacgcg cgccgtgaca tcgctcgttg gcggatcgag
     2161 cagcgcgccg tgatcggcga acgggatcgc attgagatag tagcgggtat tgtccacgtc
     2221 gaacgaggca aagccgccgt tgctactttg catgccggcg agccaatcga gcgcgcgctc
     2281 ctcggcatac gcgtagcgtc caccttcaag ggcgcgctcc atggcccacg tcaccgctgc
     2341 cgtatcgtcg aggtccggat agtagtcgtt ggcgcgctgg aacggccaac cgccgccgcg
     2401 gagtgtggca cgggcggcct gccagtctcc gggagccccg agcagctgca ggggctcgag
     2461 ccacgccagt gcgcggcgtg cggcggcgag tgcccgcgga ccgccggcct cctgcatcgc
     2521 gagcgcagcg agcgccgtat cccaaaccgg ggagacgcac ggctggcaat aggcgctcga
     2581 gacatcgacg atcaggagtc gctgcaatgc gcgcgtggcc gcagcgcggc gcggatcctc
     2641 gcgggggtag ccgagaacgg ccagcgcttc gagcgcattc accatggcgg ggaagatcgc
     2701 acccagcccg tcctcg
//
LOCUS       DATLKL010000617         3365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_75325, whole genome shotgun sequence.
ACCESSION   DATLKL010000617 DATLKL010000000
VERSION     DATLKL010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75325"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VM713_08315"
     CDS             complement(<1..1012)
                     /locus_tag="VM713_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVH84303.1"
                     /translation="MKPEQIILHNQLIGGGFGRRLEVDGVVLAARVARHVQGPVQVLW
                     SREEDIRHDRYRPYYVDHLSAALDAKGLPVAWRHTIAGAGLWALYYGEGTVKNGVDPD
                     AVTSAVDLAYPLENMEVRFARRDPRGVPTGWWRGVGATRSVFAVESFVDELAAAVQKD
                     PVRYRRALLKEPRMRAVLELAADRAGWGTALPAGSGRGVSIQYAYGSYLAQVAEVSVD
                     AQGHPRIGRVVCAMDCGQVVNPDGVRAQLEGGVTFGLSAALANEITIANGRVQQGNFN
                     DFVPLRIGEAPKVEVHLVTSSASPGGVGETGTACVSAALRNAIYAATGKRVRTLPVSR
                     GLR"
     gene            complement(1070..2149)
                     /locus_tag="VM713_08320"
     CDS             complement(1070..2149)
                     /locus_tag="VM713_08320"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVH84304.1"
                     /translation="MVTRRQFVLVAAAVGGALVLGVAARRLQHSAGIASGGRGVLNAF
                     VRIDPSGRVTLVMPKVEMGQGTYTSLPMLIAEELEVGLDAVTVEAAPPNPEVYGFVPD
                     PSADEGLKWDQSTGTSLSIIQCWTPLRQAGATARLMLIQAAARRWGVPVASCRAENGA
                     VIHAASGRSLGYGALAAAAAALPVPAAPPLKAATDFRLIGHATTRLDAPSKVDGSARF
                     GIDARPPHAKVAVIAITPVGGGSVAALADDAALSVRGVRQVVNEGDLVAVVADDTWAA
                     MQGMKVLAPRWDDGANATVQQGALVAELESAVREGGAVALSKGKLAAAARAATHVEAT
                     YHQPFLAHATLEPMNCTVDWRSGEC"
     gene            complement(2153..>3365)
                     /gene="serS"
                     /locus_tag="VM713_08325"
     CDS             complement(2153..>3365)
                     /gene="serS"
                     /locus_tag="VM713_08325"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HVH84305.1"
                     /translation="FELDVGALRALEEKRKPCAVEVDRLRAERNANAKAVGMAKGRGE
                     ESAALIARAEALTGELSAAEAALASVQAELEEWQLGLPNLLHPSVPEGSDERGNTEVR
                     RWGEPRGFRFKARDHVEIGAGLDGLDFEAAARISGARFVVMRGAVARLHRALAQFMLD
                     LHTREHGYTEVYVPYLVQRPALTGTGQLPKFEQDLFAVRGEQGFFLIPTAEVPVTNLV
                     REQILAAERLPLKLVAHTPCFRSEAGAAGKDTRGMIRNHQFDKVELVQIVRPADSYVA
                     LEELTGHAEAVLQRLEIPYRTVVLSAGDTGFGSAKTYDLEAWLPSQERYREISSCSNY
                     EAFQARRLQARWRNPHDKKPEAVHTLNGSGLAVGRTLVALLENFQNEDGSVDVPQALR
                     PWLGGLARLAP"
BASE COUNT          494 a         1248 c         1128 g          495 t
ORIGIN      
        1 ctctgaggcc gcggctcacc ggcagcgtgc ggacccgctt cccggtggcg gcataaatgg
       61 cgttgcggag cgcggcactc acgcaggcgg tgccggtctc gccgacgcct cccgggctcg
      121 cgctgctggt gacgaggtgc acctcaacct tgggcgcctc cccgatccgc agcggcacga
      181 aatcgttgaa gttgccctgc tgtacccgcc cgttggcgat tgtgatctcg ttggcgagcg
      241 ccgcgctcag tccgaaggtg accccgccct cgagctgggc gcgcacgccg tcgggattca
      301 ccacctgacc gcagtccatg gcacacacga cccggccgat gcgtgggtgt ccctgcgcgt
      361 ccacactcac ctcggcaacc tgtgccagat aactgccgta ggcatattgg atggagacgc
      421 cgcggccgct gccggccggc agggccgtgc cccagccggc ccggtcggcg gccagctcga
      481 gcaccgcacg catgcgcggc tctttgagca gcgcacgccg gtagcgcacc ggatccttct
      541 gtaccgcagc ggccagctcg tccacgaagc tctccacggc gaacacgctg cgtgtcgcgc
      601 cgaccccgcg ccaccaaccg gtgggcacgc cgcgcgggtc gcggcgcgcg aagcgcacct
      661 ccatgttctc gagcggatag gccagatcca cggccgaggt caccgcgtcc ggatccacgc
      721 cgttcttgac cgtcccctcg ccgtagtaga gcgcccagag gcccgcgccc gcgatggtgt
      781 gccgccaggc gacgggtagg cccttcgcat cgagcgccgc cgacagatga tccacgtagt
      841 acgggcgata gcggtcgtgg cggatgtcct cctcgcggct ccacagcacc tgcaccgggc
      901 cctgcacgtg acgcgcgacg cgcgccgcca gcacaacccc gtccacctcg agacgccggc
      961 cgaaaccgcc gccgatgagc tggttgtgca ggatgatctg ctcgggcttc aagccgaggt
     1021 cgccgagctt ggcgaccgcg cggtcgggcg cctgggtgcc gccccagatc tagcactcgc
     1081 cgctgcgcca gtcgaccgtg cagttcatcg gctcgagggt cgcgtgcgcc aggaagggct
     1141 ggtgataggt cgcctccacg tgcgtcgcgg cgcgcgccgc tgcggccagc ttgcctttgc
     1201 tgagcgcgac ggcaccgccc tcacgcacgg cactctcgag ctccgcgacg agcgcgcctt
     1261 gctgcacggt ggcattcgct ccatcgtccc agcgcggcgc gagcaccttc atcccctgca
     1321 tggccgccca ggtgtcgtcg gcgaccaccg cgacgagatc gccctcgttg actacctgcc
     1381 gcacgccacg caccgagagc gccgcatcgt cggcgagcgc cgcgacgctc ccgccgccca
     1441 ccggcgtgat ggcaatcacc gccaccttgg cgtgcggcgg acgcgcgtcg atgccgaagc
     1501 gcgcgctgcc atcgactttc gatggagcgt cgagccgcgt ggtcgcgtga ccgatcagcc
     1561 ggaaatccgt ggccgccttg aggggcgggg ctgccggcac gggaagcgcg gcggcggccg
     1621 ccgccaaggc gccgtagccg aggctccggc cagaggcggc gtgaatgacc gcgccgttct
     1681 cggcgcggca gctcgccacc ggcacgcccc agcgccgcgc tgcggcctga atcagcatga
     1741 ggcgggcggt cgcccccgcc tgacgcagtg gcgtccagca ctgaatgatc gacagcgaag
     1801 tgccggtcga ttgatcccac ttcaagcctt catctgccga agggtcaggc acgaaaccgt
     1861 agacctcggg gttgggcggt gcggcttcga cggtcaccgc gtcgagtccc acctcgagct
     1921 cctcggcgat gagcatcggg agcgaggtat aggtcccctg gcccatctcg accttcggca
     1981 tcaccagcgt cacgcgcccg ctcgggtcga tgcgtacgaa cgcattcagc acaccgcgtc
     2041 cgccgctcgc gatgcccgcg ctatgctgca gccgacgcgc ggcgacgccg agtaccagtg
     2101 cgccgccgac cgcagcggcg accaacacga actggcggcg cgtgaccaca ggctacggcg
     2161 cgagccgtgc gagcccacca agccacggac gcaacgcctg cggcacgtcg accgagccgt
     2221 cctcgttctg gaagttctcg agcagcgcca ccagcgtgcg cccgacggcg agtcccgatc
     2281 cgttgagtgt gtgcacggct tccggcttct tgtcgtgcgg gttgcgccag cgtgcctgca
     2341 gacgccgcgc ctgaaacgct tcgtagttgc tgcacgagga gatctcgcga tagcgctcct
     2401 gtgagggcag ccaggcctcg agatcgtagg tcttcgctga gccgaagccg gtgtcgccgg
     2461 cactcagcac cacggtgcga taggggatct cgagccgctg cagcaccgct tcagcatgtc
     2521 cggtgagctc ctcgagcgcg acgtaggagt ccgcggggcg cacgatctgc acgagctcca
     2581 ccttgtcgaa ctgatggttg cggatcattc cgcgggtgtc tttgccggcg gcgcccgcct
     2641 cggagcggaa gcacggcgtg tgcgcgacga gcttcaacgg caagcgctcc gccgccagga
     2701 tctgctcgcg caccaggttg gtcaccggca cctcggcggt ggggatgaga aagaagccct
     2761 gctcgccgcg caccgcgaac aggtcctgct cgaacttcgg cagctgcccg gtaccggtca
     2821 gcgccggccg ctgcaccaga tacggcacgt agacctcggt gtagccgtgc tcgcgcgtat
     2881 gcaggtcgag catgaactgc gcgagcgccc gatgcagccg cgcgaccgcc ccgcgcatta
     2941 cgacgaagcg cgcgccggag atgcgcgccg cggcctcgaa atcgagaccg tcgagccccg
     3001 cgccgatctc gacgtgatcg cgggccttga agcggaagcc gcgcggctcg ccccagcggc
     3061 gcacctcggt attgccgcgc tcgtcgctcc cctcggggac cgacgggtgc agcagattcg
     3121 gcagcccgag ctgccactcc tcgagctccg cctggacgga ggccagcgcg gcctcggcgg
     3181 cgctgagctc gccggtcagt gcctcggcgc gtgcgatcag cgccgctgat tcctcgccgc
     3241 gccccttggc catgccgacc gccttggcgt tggcgttgcg ctcggcacgc agtcggtcca
     3301 cctccaccgc gcagggcttg cgcttctcct ccagcgccct gagcgcgccc acatcgagct
     3361 caaag
//
LOCUS       DATLKL010000618         3563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_75354, whole genome shotgun sequence.
ACCESSION   DATLKL010000618 DATLKL010000000
VERSION     DATLKL010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75354"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VM713_08330"
     CDS             complement(<1..503)
                     /locus_tag="VM713_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84306.1"
                     /translation="MASRLARALCLAAALALPAVPALARRHDPEKLPEIRVRDLHYGD
                     ALFYYYQHDDLEALTRLEAYDQWKRMPHHEEDAQLLMGGLYLALGMHNEAGRRFEALL
                     KDPVPSGVRNVAWFYLAQVWYARGYLDRAALALARINGRMSPELESQKELLLGNVLIH
                     QGKNDEA"
     gene            complement(512..1183)
                     /locus_tag="VM713_08335"
     CDS             complement(512..1183)
                     /locus_tag="VM713_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84307.1"
                     /translation="MPDAFTTREPGRALSALRSAAALALTLALFGAAPLTDAAPPPAP
                     PPSAAPPPAATEPASQAGDAAAPASAEPAPDTRGLDEQVQGLKKDVVDLNRDLFILEE
                     ELLFPANTQVAVFLSVDVGDFFALDSVQLKMDRKEVIDYMYTPREVDALLRGGVQRLY
                     LGNLKVGQHELVAFFSGKGPSDRPYKRGASIKFEKGIGAKYLELKIDDRQRKLQPEFE
                     IRDWE"
     gene            complement(1195..1749)
                     /locus_tag="VM713_08340"
     CDS             complement(1195..1749)
                     /locus_tag="VM713_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HVH84308.1"
                     /translation="MSLQRPDGPAARDRRSRVRLFLQCAAVVLLGGGTGALAQRVYGL
                     IARAAPDFTLHAVAGGNVRLSEHRGEVVVLSFWSSGCGTCRSQLDALNRSLATYHSAG
                     LEVYGVGLDDVPARAADYARGVPVAFPMLLDPSKAVGRAYEVDNLPMTVLIDRNGTIR
                     NVLRDFSMKSDDVYLQQLRVLLNE"
     gene            complement(1925..>3563)
                     /locus_tag="VM713_08345"
     CDS             complement(1925..>3563)
                     /locus_tag="VM713_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84309.1"
                     /translation="MLTAKRPPRPNPPGTPFRHENHPRPVTRRDFLAAGMLGAQGMVL
                     GSAWLGSLLKAQAARAAGLDADISALLAQGQCNVPSGSGSLPFIVFDLAGGANLVGSE
                     ALVGVQGGQANFLSTAGFGKLGVPGNMVPTSSTFISSALGLLWHSDGAILRGILTKAS
                     AATQAGTNGAVIPAMSQNDTQSNTINPIYGIAEAGAKGTVVNLVGTDSSVSGGNSMAL
                     MYTIQPALQPTTIQSPADAIALAPVPPGGGPPDPLAIAELESETRISAGTSPYQGAGS
                     TGSEFSGALSNPSGSTPGVQLIPGGGSSDAALKNQVRCSYVKAANTANIFAAGPAGLD
                     PTQDPLIVGGTTPIFTATDFQSQDVQMTASVMKLVIDGYAGAGTISLGGYDYHDGSRA
                     TGETRNFTAGQMIGAVLEYASRKGTPVMIQIMSDGSLAANSMVDTSTAGRNKLGTQGD
                     NSSVAATLFLVYSPNGRPVLRNGVAGQQIGYFSSDGSVVSSSSPAANSINQIPELIML
                     NYMGLIGTDAQFPTLFPMQGLGPAASLAAMTVFQRII"
BASE COUNT          547 a         1167 c         1284 g          565 t
ORIGIN      
        1 atcgcctcgt cgttcttccc ctgatggatg agcacgttgc cgagcagcag ctccttctgc
       61 gactcgagct cgggcgacat gcggccgttg atgcgcgcca gcgccagcgc ggccctgtcg
      121 agatagccgc gcgcgtacca cacctgcgcc aggtagaacc aggcgacgtt gcgcacgccg
      181 ctcggcaccg gatccttgag cagcgcctcg aagcgccggc cggcctcgtt gtgcatgccg
      241 agcgcgaggt acaacccgcc catcagcagt tgtgcgtcct cctcgtggtg cggcatgcgc
      301 ttccactggt cgtaggcttc gaggcgcgtg agcgcctcga gatcgtcgtg ctggtagtaa
      361 tagaagagcg cgtcgccgta atgcaggtcg cggacgcgga tctcgggaag cttctccgga
      421 tcgtggcgcc gcgcgagcgc cggcacggcc gggagcgcca gggccgccgc caggcagagc
      481 gcgcgtgcga gccgggaagc catgcgtggc gctactccca gtcccggatc tcgaattccg
      541 gctggagctt tctttgccgg tcgtcgatct tgagctcgag gtacttcgct ccaatgccct
      601 tctcgaactt gatcgaggcg ccgcgcttgt agggccggtc gctcgggccc ttgccgctga
      661 aaaaggcgac cagctcgtgc tggccgacct tgaggttgcc gaggtagagc cgctgcacgc
      721 cgcccctgag aagcgcgtcc acctcgcggg gcgtgtacat atagtcgatg acttccttgc
      781 ggtccatctt cagctgcacc gagtcgagcg cgaagaagtc gccgacgtcg accgacaaaa
      841 acaccgcgac ctgggtgttg gcagggaaca gcagctcctc ctcgagaatg aagaggtcgc
      901 ggttgaggtc gacgacgtct ttcttcaatc cttgcacctg ctcatcgagg ccgcgcgtgt
      961 ccggcgccgg ctcggcggat gccggcgcgg ccgcatcgcc ggcctgcgag gcaggctctg
     1021 tcgcagccgg cggcggggcg gcggacggcg ggggtgccgg cggcggcgcc gcatcggtga
     1081 gcggcgcggc accgaacagt gcgagggtga gggcgagcgc ggcggcgctt ctcaaagcgc
     1141 tcagcgcacg gccgggctcg cgagtcgtga aggcatcggg catggtcgtt ccgatcactc
     1201 gttcagcaga acccgcaact gctgcaggta aacgtcgtcg ctcttcatgc tgaagtcgcg
     1261 cagcacgttg cggatggtgc cgttgcgatc gatcagcacg gtcatcggca gattgtccac
     1321 ctcgtaggcg cggcccacgg ccttgctcgg atcgagcagc atcgggaagg ccaccgggac
     1381 gccgcgcgca taatcggccg cgcgcgccgg gacgtcgtcc agacccacgc cgtatacctc
     1441 gagcccggcg gagtggtagg tggcgagact cctgttcaga gcgtcgagct gactgcggca
     1501 ggtgccgcag ccgctgctcc agaagcttaa gaccacgacc tcgccgcgat gctccgagag
     1561 acgcacattg cccccggcga cggcgtgcag cgtgaaatcc ggggcggccc gcgcgatgag
     1621 cccgtaaacg cgctgcgcga gcgcgcccgt gccgccgccg agcagcacca cggcagcgca
     1681 ctggagaaag agccgcacgc gtgaccggcg gtcacgcgcc gccggaccgt cgggtcgttg
     1741 caagctcaag atgaagatcc ttcgggccgt gaggccgagc tgcgcgcaag cgcgggaacc
     1801 ctcgcacagc gcagtcgaga ctgtcaacgc agcagggcgc gaaatggctc gcggcggcgc
     1861 agcgcaggtc cgcgcagtcg tcacacggcg acaggtgccg ctcggccgcg tccgaccgcg
     1921 cgctttaaat gatccgctgg aacaccgtca tggccgcgag cgacgccgcc ggcccgagtc
     1981 cctgcatggg gaagagcgtc gggaactgcg cgtccgttcc tatcagtccc atgtagttga
     2041 gcatgatgag ctcggggatc tggttgatgg agttcgccgc ggggctcgag ctcgagacca
     2101 ccgagccgtc ggagctgaag tagccgatct gctgcccggc gacgccgttg cgcagcaccg
     2161 ggcggccgtt cgggctgtag accaggaaca gtgtggccgc caccgaggag ttgtcgccct
     2221 gcgtgccgag cttgttgcgc ccggcggtgc tcgtgtcgac catgctgttg gcggcgagcg
     2281 agccgtcgct catgatctgg atcatcaccg gcgtgccctt gcgcgaggcg tactcgagca
     2341 cggcgccgat catctgcccg gcggtgaagt tgcgcgtctc gccggtggcg cgcgagccgt
     2401 cgtgatagtc gtagccaccg agcgagatcg ttcccgcgcc cgcgtaaccg tcgatcacga
     2461 gcttcatgac cgaggccgtc atctgcacgt cctggctctg gaagtcggtc gcggtaaaga
     2521 tcggcgtggt gccgcccacg atgagcggat cctgggtcgg atcgagcccg gcgggaccgg
     2581 cggcaaagat gttggccgtg ttggcggcct tcacgtacga gcagcgtacc tggttcttga
     2641 gcgccgcatc ggagctcccg ccgccgggga tgagctgcac gcccggagtg gagccgctcg
     2701 ggttgctgag cgcgccgcta aactcgctgc cggtggagcc ggcgccctga taagggctcg
     2761 tgccggcgct gatgcgcgtc tccgattcga gctcggcgat cgcgagcgga tcgggcgggc
     2821 cgccgccggg cggcacgggc gcgagtgcga tcgcatccgc ggggctctga atcgtggtgg
     2881 gctgcagcgc gggctgaatc gtgtacatga gcgccatcga gttgccgccc gacaccgagg
     2941 agtccgtgcc gaccaggttg accaccgtgc ccttggcgcc ggcctccgcg atgccgtaga
     3001 tcgggttgat agtgttcgac tgggtgtcgt tctgcgacat cgccgggatg accgcgccgt
     3061 tggtgccggc ctgagtcgca gccgaagcct tggtgagaat gccgcggagg atcgcgccgt
     3121 cggaatgcca caggagcccg agtgcgctcg agatgaaggt gctggaggtc ggcaccatgt
     3181 tgccgggcac gccgagcttg ccgaagccgg cggtcgagag gaagttcgcc tgtccgccct
     3241 gcacgcccac cagcgcctcg gagccgacca ggttcgcgcc tcccgcgagg tcgaacacta
     3301 tgaaggggag cgaccccgag cccgagggca cgttgcactg cccctgggcg agtagcgccg
     3361 agatatcggc atcgagcccg gcggcccggg cggcctgcgc cttgaggagc gagccgagcc
     3421 aggcggatcc gagcaccatg ccttgggcgc ccagcatgcc ggccgcgagg aagtcgcggc
     3481 gggtcaccgg gcgcgggtgg ttctcgtgcc ggaagggagt gccgggcggg ttgggccgcg
     3541 gtgggcgctt cgctgtcagc atc
//
LOCUS       DATLKL010000619         1706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_210479, whole genome shotgun sequence.
ACCESSION   DATLKL010000619 DATLKL010000000
VERSION     DATLKL010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210479"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VM713_08350"
     CDS             <1..299
                     /locus_tag="VM713_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HVH84310.1"
                     /translation="KQIRAIVDIQLIYLRKRLQERNMDLALDDAARDLLGESGFDPVY
                     GARPLKRAIQQQIENPLAQRILEGEFQPGDRIRIAVREGQLTFGKVEERKAARA"
     gene            390..803
                     /locus_tag="VM713_08355"
     CDS             390..803
                     /locus_tag="VM713_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HVH84311.1"
                     /translation="MPFYEYECQACKFYTEVMQKISDAPLTKCPSCGKRRLKKLVSAP
                     VFRLKGGGWYETDFKSDKEAKRNLLGADKEEAKAESKPESKDGKPEAKDAKAADAKAA
                     DAKTPQAKPAEAKSSSGERRAAKSVRRRSNRRPRR"
     gene            825..1505
                     /locus_tag="VM713_08360"
     CDS             825..1505
                     /locus_tag="VM713_08360"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HVH84312.1"
                     /translation="MPATESSVKKPEGAPRKWTLRRYLVAGVLVWLPILATIWVVNFM
                     LHLMDRTLLLLPPAYRPEALLGFKIPGAGLLLALVLLLLTGLLVTNLIGRRLVVWGEE
                     LLNRIPVVRTVYGGVKSFAESVFSQSNSFRKVVMVEYPREGAWSIGFLTAEDVPEISQ
                     KTGETHVAVYVSSALNATAGWLIVVPRRMVRELDMSVDAALKMIITCGVVVPPHPAVK
                     SQVAKPAA"
     gene            1520..>1706
                     /locus_tag="VM713_08365"
     CDS             1520..>1706
                     /locus_tag="VM713_08365"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVH84313.1"
                     /translation="MRLTAPGAPLELTELSLHAPGEHELLIAVEACGVCRTDLHVLDG
                     ELPDVRCPLTLGHEVVGR"
BASE COUNT          306 a          538 c          591 g          271 t
ORIGIN      
        1 cgaagcagat ccgcgcgatc gtcgacatcc agctcatcta cctgcgcaag cggctgcagg
       61 agcgcaacat ggacctcgcg ctggacgatg ccgcacggga tctgctcgga gaatcggggt
      121 tcgatccggt gtatggggcg cggccgctga agcgggctat ccagcagcag atcgagaatc
      181 ccctcgcgca gcggatcctc gagggcgagt tccagcccgg cgaccgcatc cgcatcgctg
      241 tccgtgaggg acagctcaca ttcggtaaag tcgaggagcg caaggccgcg cgcgcctgag
      301 atgcgccgtg cgctgacggg cgcgggacgt gcgcgtgcgt ccgttataat ccgcaccggt
      361 taaattcagg ggttccgacc aagcagccca tgccgttcta cgagtacgag tgccaggctt
      421 gcaagttcta caccgaggtc atgcagaaga tcagcgacgc gccgctgacc aagtgcccgt
      481 cgtgcggcaa gcgccgcctg aagaagctgg tgtcggcccc ggtgtttcgc ctcaagggcg
      541 gcggttggta cgagaccgac ttcaagtccg acaaggaggc gaagcgcaac ctcctcggcg
      601 cggataaaga agaggcgaag gcagagagca agccggaatc caaggacggc aagcccgagg
      661 cgaaggacgc caaggcggcc gatgccaagg cggccgatgc caagactccc caagcgaagc
      721 cggcagaggc gaagtcctcg agcggcgagc gccgtgcggc aaagagcgtt cggagaagga
      781 gcaaccggcg gccgcggcgt taaggagccg cgcgggagga atccatgcca gcgactgaaa
      841 gcagcgtgaa gaagccggaa ggcgctccca gaaaatggac cttgcggcgc tatctggtgg
      901 caggggttct agtatggctg ccgatcctcg ccaccatctg ggtggtgaac ttcatgctgc
      961 acctcatgga ccggacgctg ctgctcctgc cgccggcgta ccggcccgag gcgctgctcg
     1021 ggttcaagat ccccggtgcg gggctcctgc tggcgctggt gctgctgctg ctcaccgggt
     1081 tgctggtgac taacctgatc ggccggcgcc tggtggtgtg gggcgaggag cttctcaacc
     1141 gcattccggt ggtgcgcacc gtgtacggcg gagtgaagag cttcgccgag agcgtcttct
     1201 cgcaatccaa ttccttccgc aaggtcgtga tggtggagta tccgcgcgag ggcgcctgga
     1261 gcatcggctt cctcacggcc gaagatgttc cggagatcag ccagaagacc ggcgagaccc
     1321 acgtggctgt gtatgtctcc tcggcgctca atgccaccgc gggctggctc atcgtggtgc
     1381 cgcggcgcat ggtgcgcgag ctcgacatgt cggtggacgc cgcgctcaaa atgatcatca
     1441 cctgcggcgt ggtggtgccg ccccatccgg cggtgaagtc gcaggtggcc aagccggcgg
     1501 cctgacgccc gtgaaggcca tgcgcctcac tgcgcccggt gcgccgctcg agctcaccga
     1561 gctctccctg cacgcgcctg gcgagcacga gctcctcatc gccgtcgaag cctgcggcgt
     1621 gtgccgcacc gacctccatg tgctcgacgg ggagctgccg gacgttcgct gtccgctcac
     1681 cctgggtcac gaagtcgtcg gtcggg
//
LOCUS       DATLKL010000620         1931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_41385, whole genome shotgun sequence.
ACCESSION   DATLKL010000620 DATLKL010000000
VERSION     DATLKL010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41385"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VM713_08370"
     CDS             complement(<1..733)
                     /locus_tag="VM713_08370"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HVH84314.1"
                     /translation="MSDQARKARQFHELHIPGTPLVLFNVWDAGSAKAVTAGDAKAIA
                     TSSWSVAHANGFADGEQLPLPIAIENLRRIVDVTELPVTVDLESGYGEIPEAVASSIA
                     LALDVGAVGCNLEDSVPADGRLRTAAGQTVRIRGARRAAEAAGLPFFINARTDVFFQR
                     PTAQHDDAMVSEALERAQRYRDAGADGPFAPGLVDIRLIARLAAASPLPLNVVVQQGT
                     PALKVLADHGVGRVSHGPEPYFLAMK"
     gene            909..1736
                     /locus_tag="VM713_08375"
     CDS             909..1736
                     /locus_tag="VM713_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVH84315.1"
                     /translation="MELSTQLLLDTPALAVRDVVCAGTCRHRSEEEFAQTTCLVFPYR
                     GVYVRHVGRSDSVAEANQLLFFNQGEGYRVSHPVEGGDACLSLAIGETWLRELAAPLE
                     LQDGVALAFRRQRLRIDARTQALVALVRHGLKTQRAETLEAETLVLTLVRRALGERTS
                     HAPGGSSGRQKLVDRAKLVLSSELGRRWTLGDIAAEVGVSPVYLTQVFQQVEGLPLYR
                     YQLRLRLARALHLLGRCEDLSRLSLDLGFSSHSHFTSAFGQLYGRTPAEFRRSLHRH"
BASE COUNT          307 a          661 c          620 g          343 t
ORIGIN      
        1 ctttcatcgc cagaaaataa ggctcgggcc catggctgac gcgcccaacg ccgtgatccg
       61 caagtacctt gagcgccggt gtgccttgct ggaccacgac gttgagcgga agtggcgatg
      121 ccgcggccag ccgtgcgatc aggcggatgt cgacgaggcc tggtgcaaag ggtccgtcgg
      181 cgcccgcgtc gcgatagcgc tgcgcgcgct cgagcgcctc gctcaccatc gcgtcgtcgt
      241 gctgcgcggt cgggcgctga aagaagacgt ccgtgcgcgc gttgatgaag aagggaaggc
      301 ccgccgcctc ggccgcgcgc cgtgcgccgc ggatccgtac cgtctggccc gcggcagtac
      361 gcagccggcc atcggcgggc acgctgtcct cgaggttgca tcccaccgcg ccgacgtcga
      421 gcgcgagcgc gatcgagctg gccacggcct ctggaatctc cccataaccg ctctcgaggt
      481 ccaccgtcac cgggagctcg gtcacgtcga cgatgcgacg taggttctcg attgctatgg
      541 gaagcggcag ctgctcgccg tccgcgaatc cgttcgcatg tgcgaccgac caactactcg
      601 tcgcgatcgc cttcgcatcg ccagcggtta ccgccttcgc gctccccgcg tcccagacat
      661 tgaacaggac gagcggcgtc cctgggatat gcagttcatg gaattgccgc gccttgcgcg
      721 cttgatccga cataactttc accttgattg atccgtcagg tgtagttgag cacaccgtcc
      781 gatgacgagg ctgtcacgat ctttagcaat cgggcacgga cgctacgctg ggggcgcttc
      841 gcagaatcgg cgtcaggatt cgctgggcct ggaatttgta cctacaatcg gcagccggat
      901 cacccctgat ggaactctcg acgcaactgc tcctcgacac gccagcgctc gccgtgcgtg
      961 acgtcgtgtg cgcgggcacc tgccggcacc ggagcgagga ggagtttgcg cagacgacct
     1021 gcctcgtgtt cccgtatcgc ggggtctacg tgcgccacgt cggtcgcagc gactccgtgg
     1081 ctgaggccaa tcagctcctc ttcttcaatc agggagaggg ttaccgcgtc agccacccgg
     1141 tcgagggggg cgacgcctgc ctatcactcg cgatcgggga gacctggctg cgcgagctcg
     1201 cggcgccgct cgagctccag gacggggtcg ctctagcgtt ccgccgtcag cgattgcgga
     1261 tcgacgcgcg cacccaggcc ttggtcgcgc ttgtccgcca cggcctcaag acccaacgcg
     1321 ccgagactct ggaagcagaa accctggtgc tgacgctcgt gcgccgcgcg ctcggcgagc
     1381 gtacctcgca cgctccaggc gggagctccg ggcggcagaa gcttgtcgat cgcgcgaagc
     1441 tcgtcctgtc ctctgaactc gggcggcgct ggacgctggg cgacatcgcg gctgaggtcg
     1501 gtgtgtcgcc cgtgtatctc acacaggtgt tccagcaggt agaaggtctg ccactgtacc
     1561 ggtatcagct gcgtctgcgt cttgcgcggg cgctccatct cctggggcgc tgcgaggatc
     1621 tctcccgcct cagcctcgat ctcggattct caagccacag tcatttcacc tccgcgttcg
     1681 gccagcttta cggccggaca cctgccgagt tccgccgctc cctccatcgg cattaagtcc
     1741 ggcggcccat ggcctgccgg tgccaggcga cgggttacgt gactcagcgg ctgccggcgg
     1801 gcgctcgacc gagcaacagc ccatattcgg ttccgagcac gcactcaagg agtccgcaac
     1861 gcagaaggag cgagaggtga tcgaaacccg gaggccgtgc tcccacgcct gaactccgcc
     1921 ctatcgctac c
//
LOCUS       DATLKL010000621         1735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_165493, whole genome shotgun sequence.
ACCESSION   DATLKL010000621 DATLKL010000000
VERSION     DATLKL010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165493"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VM713_08380"
     CDS             <1..196
                     /locus_tag="VM713_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003171120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84316.1"
                     /translation="ETRGLPARVLALVPRARQLAIPMRARNRSLNLSNAVALVVYEAW
                     RQNGFAGAAAGCQGRDDGKA"
     gene            215..889
                     /locus_tag="VM713_08385"
     CDS             215..889
                     /locus_tag="VM713_08385"
                     /inference="COORDINATES: protein motif:HMM:NF036878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HVH84317.1"
                     /translation="MSERVLLVDYENIGKIDLAAIPDGVRVPFFFGASQRTVPTEFLK
                     AALKLGERFVPIDIEGQGKNALDFHIAFYLGEYLARAPDTACVILSKDKGFDPLVRHL
                     SRRGFAVRRANSMAEALAARTSAAAASRSPGGARSGARRESGRDGPRRDGPPSEGGSL
                     KGEALHLLAGTQKVRRPRKRKGLIAVLHSHFSQKVPDGELQRLVDQLIAEGTLSETNG
                     AITYHF"
     gene            971..>1735
                     /locus_tag="VM713_08390"
     CDS             971..>1735
                     /locus_tag="VM713_08390"
                     /inference="COORDINATES: protein motif:HMM:NF021454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HVH84318.1"
                     /translation="MSRSRHFGRCLSAAALLLTLLSWPAFAGAEEADAPRQAIAAARD
                     AARAALERGPVTVTLRDQAKLNLPEGFGFIPQQQAGALMSAMGNRTGDNLLGLIVPLG
                     VPARWLVVASYNPSGYIKDDDAQHWDADKLLQSLKDGTEAANAERAKVGVPALKVTRW
                     IEPPAYTADTHRLIWSAEALRKDGTTDDPTINYNTYVLGREGYISLNLITSGSAVEND
                     KDSAGALLSAVSFNDGKRYADFNSSTDKVAAYGLAAL"
BASE COUNT          277 a          613 c          582 g          263 t
ORIGIN      
        1 cgaaacccgg ggcctgccgg cgcgggtgct cgcgctcgtg ccgcgcgcgc ggcagctggc
       61 gatcccgatg cgcgcgcgca atcgaagctt gaatctctcg aatgcggtgg ctctggtggt
      121 gtacgaggca tggcggcaga atggcttcgc cggcgcggcc gccggctgtc agggcaggga
      181 tgacggcaaa gcctgagcgg gagtgagaac gtgcgtgtct gaaagagttc tgctcgtcga
      241 ctacgagaac atcggcaaga tcgatctcgc cgcgatcccc gacggggtgc gggtgccgtt
      301 cttcttcggt gcctcgcagc gcacggtgcc gaccgagttc ctcaaggcgg ctctcaagct
      361 cggcgagcgc ttcgtgccga tcgacatcga gggacagggc aagaacgcgc tcgacttcca
      421 tatcgccttc tatctcgggg agtacctcgc gcgcgcgccc gacaccgcct gcgtcattct
      481 ctccaaggac aagggcttcg atcccctggt tcgtcacctc agtcgccgcg gcttcgccgt
      541 gcgccgtgcc aattcgatgg ccgaggcgct cgccgcgcgc acctccgcgg cggccgcgtc
      601 ccgcagtccc gggggcgcgc gctcgggtgc gcggcgcgag agcggccgag acggtccgcg
      661 ccgagacggc ccgccgagcg agggcgggtc gctgaagggc gaagcgctgc acctgctcgc
      721 cggcactcag aaggtgcgcc gtccgcgcaa gcgcaagggc ctgatcgcgg tgctgcactc
      781 gcacttttcg cagaaagtcc ccgacggcga gctgcagcga ctggtcgatc agctgatcgc
      841 cgagggcacg ctgagcgaga caaatggtgc gattacgtat catttctgac agctcgtgat
      901 acccccgccg cgccgccccg tgtcagagtg ggcgccccca cagtgtgggc gtgtggtgag
      961 gaggccttgc atgtcccgat cccgacactt cggccgctgc ctgtcggccg cggcgctgct
     1021 gctgacactg ctctcctggc cagctttcgc aggcgccgag gaggccgacg ctccgcgcca
     1081 ggcgatcgcc gcggcccgtg acgcggcgcg cgcggcgctc gagcgcggac cggtcaccgt
     1141 cacgctgcgc gatcaggcga agctgaacct cccggaggga ttcggcttca tcccgcagca
     1201 gcaggccggg gcgctcatga gcgccatggg caatcgtacc ggcgataatc tcctcgggct
     1261 catcgtcccg ctcggcgtgc cggcccgctg gctcgtcgtc gcctcctaca acccgtccgg
     1321 ctacatcaag gacgacgacg cgcagcactg ggacgccgac aaactgctgc agagcctcaa
     1381 ggacggcacc gaggccgcca acgccgagcg tgccaaggtc ggagtgccgg cgctcaaggt
     1441 gacgcgctgg atcgagcctc ccgcctacac cgcagacacg caccggctga tctggtccgc
     1501 cgaggcgctg cgcaaggacg gcacgacgga cgaccccacg atcaactaca acacctacgt
     1561 cctggggcgc gaaggctaca tctcgctcaa tctcatcacc tccggctccg cggtcgagaa
     1621 cgacaaggac tccgccggcg cgctgctctc ggccgtgagc ttcaacgacg gcaagcgcta
     1681 tgccgacttc aattcctcca ccgacaaggt ggccgcgtac ggactcgccg cgctg
//
LOCUS       DATLKL010000622         3186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_52573, whole genome shotgun sequence.
ACCESSION   DATLKL010000622 DATLKL010000000
VERSION     DATLKL010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52573"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1333
                     /gene="metK"
                     /locus_tag="VM713_08395"
     CDS             161..1333
                     /gene="metK"
                     /locus_tag="VM713_08395"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HVH84319.1"
                     /translation="MSNDYVFTSESVSEGHPDKVADQISDAVLDAILKEDARGRVACE
                     TLVKTGVVIVAGEVTTSAWVDVEALVRKTVLEIGYDSSDMGFDGASCGVLNIIGKQSP
                     DIAQGVDRKDERKQGAGDQGLMFGYATNETDVLMPAPITLAHRLVKRQAQVRKAGKLP
                     WLRPDAKSQVTLRYEDQEPVGIEAVVLSTQHSPEVSTKALREAVMEEILKPVLPAKWV
                     DKRTRYHINPTGRFVIGGPVGDCGLTGRKIIVDTYGGYARHGGGAFSGKDPSKVDRSA
                     AYAARYVAKNIVAAGLAERCEVQVSYAIGVAEPTSIMVETFGTSRLSREHLTQLVRKH
                     FDLTPYGLREMLDLVRPIYQKTATYGHFGRTEPEFTWERTDRAAQLADEAKGARAA"
     regulatory      1369..1465
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            1482..2903
                     /gene="ahcY"
                     /locus_tag="VM713_08400"
     CDS             1482..2903
                     /gene="ahcY"
                     /locus_tag="VM713_08400"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HVH84320.1"
                     /translation="MNATLKPAAAKSDHRVADLKLADWGRKEIAIAETEMPGLMAIRE
                     EFAPTQPLRGARISGSLHMTIQTAVLIETLQALGAEVRWASCNIFSTQDHAAAAIAAR
                     GTAVFAWKGESLDDYWEYTHRIFAWPDGGHSNMILDDGGDATLLLHLGVRAENDAGVI
                     SKPASDEERALFAAIRAHLKSDPKWYSSRIGHVKGVTEETTTGVKRLYQMAKDGKLAF
                     PAINVNDSVTKSKFDNLYGCRESLVDAIKRATDVMIAGKIAVVCGYGDVGKGCAQALR
                     ALSAQVWVTEIDPICALQAAMEGYRVVTMDYAADQADIFVTATGNFHVITHDHMKRMK
                     HNSIVCNIGHFDSEIDVASLKQYSWENIKPQVDHVILPDGRRLILLAEGRLVNLGCGT
                     GHPSYVMSSSFANQVIAQVELFTNPHKYPVGVYVLPKLLDEKVARLQLQKLNVELSRL
                     TDAQARYIGVDRGGPYKPDHYRY"
     gene            2903..>3186
                     /locus_tag="VM713_08405"
     CDS             2903..>3186
                     /locus_tag="VM713_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HVH84321.1"
                     /translation="MSAATTEAVRTAGGALLLDGKAVGARVRAQVAERAAAFAALHGR
                     RPGLAVVQVGEDPASSVYVRNKRKSSLEAGIESFAHDLPADTGEAQILALV"
BASE COUNT          578 a         1075 c         1067 g          466 t
ORIGIN      
        1 catgggggcc atcaaggtcg agacgcgcgg cacgcagaac caccatttca cccgggccgc
       61 attcgacgag cggctcgcgc gcgcaatggc ggagcggccc ggcgcccacc tgggttaaac
      121 tgattgcttt ggcagcacgc gcgcggctga ggaattaaag atgtcgaatg actatgtctt
      181 cacctccgag tccgtctccg agggccatcc ggacaaggtc gccgaccaga tctcggatgc
      241 ggtcctcgac gcgatcctga aggaggatgc gcgcggccgc gtcgcctgcg agacgctggt
      301 caagaccggg gtggtgatcg tagcgggcga ggtcacgacg tccgcctggg tcgacgtcga
      361 ggcgctggtg cgtaagaccg tgctcgagat cggctacgac tcctccgaca tgggtttcga
      421 cggcgccagc tgcggtgttc tcaacatcat cggcaagcag tcgcccgata tcgcccaggg
      481 cgtcgaccgc aaggatgagc gcaagcaggg cgccggcgat caggggctga tgttcggcta
      541 cgccaccaac gagaccgacg tgctgatgcc cgcgccgatc acgctggccc accgcctggt
      601 caagcgccag gcgcaggtgc gcaaggcggg caagctcccc tggctgcgcc cggacgccaa
      661 gagccaggtc acgctgcgct acgaggatca ggagcccgtc ggcatcgagg cggtggtgct
      721 gtcgacccaa catagcccgg aagtctcgac caaggcgctg cgcgaggcgg tgatggagga
      781 gatcctgaaa cccgtgctgc ccgcgaagtg ggtcgacaag cgcacgcgct atcacatcaa
      841 tcccaccggg cgcttcgtga tcggcggacc ggtcggcgac tgcgggctca ccgggcgcaa
      901 gatcatcgtc gacacgtatg gcggttacgc ccgccacggc ggcggcgcct tctccggcaa
      961 ggacccctcc aaggtcgatc gctcggcggc ctatgcggcg cgctacgtcg ccaagaacat
     1021 cgtcgccgcg gggctcgccg aacgctgcga ggtgcaggtg tcgtacgcca tcggcgtggc
     1081 cgagccgacc tcaatcatgg tggagacctt cggcacctcc cgcctgtcgc gcgagcatct
     1141 gacgcagctc gtgcgcaagc acttcgacct gacaccctac gggctgcgcg agatgctcga
     1201 cctggtgcgc ccgatctacc agaagaccgc gacctacgga cacttcggcc gtaccgagcc
     1261 ggagttcacc tgggagcgca ccgatcgcgc ggcacagctg gccgatgagg ccaagggagc
     1321 acgcgcggcc tgaggcttac cttgagccgc acactgcggc gcacacggca cgctgaggag
     1381 cgttgcgacg ggtcggtggc gctcgcgtcc accgcgcctt gccaggctca gcggccgctg
     1441 tcatccaaca cggcgctcgc tctcactacg gagtaccgag tatgaacgcc acactgaaac
     1501 ccgccgccgc gaagtccgac caccgcgtcg ccgacctcaa gctcgccgac tggggccgca
     1561 aggagatcgc gatcgccgag accgagatgc cgggcctcat ggcgatccgc gaggaattcg
     1621 ccccgaccca gccgctgcgc ggcgcgcgca tttccgggtc gctccacatg acgatccaga
     1681 cggcggtgct gatcgagacc ctgcaggccc tcggcgccga ggtccgctgg gcctcgtgca
     1741 acatcttctc cacgcaggat cacgcggcgg cggccatcgc cgcgcgcggc acggcggtat
     1801 tcgcctggaa gggcgagtcg ctggacgact actgggagta cacgcaccgc atcttcgcct
     1861 ggcccgacgg cgggcattcc aacatgattc tcgacgacgg cggcgatgca acgctgctgc
     1921 tgcacctcgg cgtgcgcgcc gagaacgacg ccggcgtgat cagtaagccg gcgagcgacg
     1981 aggagcgggc gctgttcgcc gcgatccgcg cgcacctgaa gagcgatccc aagtggtact
     2041 cgagccgcat cggccacgtg aagggcgtga ccgaggaaac caccaccggc gtgaagcgtc
     2101 tctaccagat ggcgaaggac ggcaagctcg cctttcccgc gatcaacgtc aacgattcgg
     2161 tcaccaagag caaattcgac aacctgtacg gctgccgcga atccctggtc gatgccatca
     2221 agcgcgccac cgacgtgatg attgccggca agatcgccgt ggtgtgcggt tacggcgacg
     2281 tcggcaaggg ctgtgcgcag gcactgcgcg cgctgtcggc gcaggtatgg gtcaccgaga
     2341 tcgatccgat ctgcgcgctg caggcggcca tggaaggcta ccgcgtcgtg accatggatt
     2401 acgcggccga ccaggccgat atcttcgtca ccgccacggg gaacttccac gtgatcacgc
     2461 acgatcacat gaagcgcatg aagcacaatt cgatcgtgtg caacatcggc cacttcgaca
     2521 gcgagatcga cgtggcgagc ctcaagcagt attcgtggga gaacatcaag ccacaggtcg
     2581 atcacgtgat cctgcccgac ggccggcggc tgatcctgct ggcggaggga cggctggtga
     2641 acctcggctg cggcaccggt cacccgagct acgtgatgag ctcgagcttc gccaaccagg
     2701 tcattgcgca ggtcgagctc ttcaccaacc cgcacaagta cccggtgggg gtgtacgtgc
     2761 tgccgaagct gctcgacgag aaagtggcgc ggctgcaact gcagaagctc aacgtcgagc
     2821 tctcgcggct caccgacgcg caggcgcgct acatcggcgt ggaccgcggc ggcccctaca
     2881 agcccgacca ctaccgctat tgatgagcgc cgcaacgaca gaggcggtgc gcactgcggg
     2941 cggcgcgctg ctcctcgacg gcaaggcggt cggcgcacgg gtgcgggcgc aggtcgccga
     3001 gcgggccgca gccttcgccg cgctgcacgg acgccggccc gggctcgcgg tagtgcaggt
     3061 gggtgaggat ccggcctcga gcgtctacgt gcgcaacaag cgcaagtcct cgctcgaggc
     3121 cgggatcgag tctttcgccc acgacctgcc cgccgacacg ggcgaagcgc agatcctcgc
     3181 gctggt
//
LOCUS       DATLKL010000623         2761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_18717, whole genome shotgun sequence.
ACCESSION   DATLKL010000623 DATLKL010000000
VERSION     DATLKL010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18717"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VM713_08410"
     CDS             complement(<1..542)
                     /locus_tag="VM713_08410"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HVH84322.1"
                     /translation="MRVRRAARAAAACSAMALAPTAALPEPQPLWELGLGAGALAFND
                     YRGADTTHVYPLPVPYFVYRGKFLESDRDGLRGKLFKQDRIELNLSVNATTPVRNDSA
                     RAGMPDLRSTLEIGPALSAHLWRSADRRLKLDLRLPARAAFTIEANPRGIGWFYAPNL
                     NLDVAQHAGAGGWKLGLLAGP"
     gene            complement(539..1591)
                     /locus_tag="VM713_08415"
     CDS             complement(539..1591)
                     /locus_tag="VM713_08415"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84323.1"
                     /translation="MTRAPAGLHLDYLSQAEYRAARWADVLGVASFNGPPAPSPDAPE
                     IPVAEVNTPVLLGTAAVYEVWRSGQPAQSGRRNRVRFRRSENLLFGCIALAESEVAPA
                     LPDVPAAPSALHAATEQAYREICATLDALGYPHLLRVWNYLPDINRDSDGIERYRQFN
                     QARQHALSACGRAIRGNVPAASALGAASGSPLVVYFLAGRAAPAFIENPRQLSAYRYP
                     RQYGSHSPVFSRATLLRQRDGLTLFISGTASIVGHRSIHVGDTAAQTRETLTNIAALL
                     DEANRVEGAARFSLGSLACKVYVRRPADLPVIRAELAAALEPTSRVIYLQADICRQDL
                     LVEIEATGLCPLKTDA"
     gene            complement(1608..1994)
                     /locus_tag="VM713_08420"
     CDS             complement(1608..1994)
                     /locus_tag="VM713_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84324.1"
                     /translation="MKANLTLTAVATALALSLATAALAQETQGAGVKETVKEDAKTFG
                     HAVEHGAKTVGHAVADTSRKVGHEVADTSRKVGHEIAEKTAPAREAVAEDSKKAGHAI
                     AHGAKAVGQKAKDAVTSKSDDSKPKN"
     gene            2115..>2761
                     /locus_tag="VM713_08425"
     CDS             2115..>2761
                     /locus_tag="VM713_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVH84325.1"
                     /translation="MLRTRYVPTGWDVLAFILVFAFFVYCAQAAHGLTGSLSGLEATP
                     ISLDPSALFGYSARTALRMLLAMVASLLFTFTYATLAAKSHRAEIILVPLLDFLQSVP
                     ILSFSITILFFLQLTPGRVAGAELASIFLIFTSQAWNMAFSFYHSLRSIPDELVEAAR
                     NFQLGPWMRFWRLEVPFAMPALIWNMMMSMSGGWFFVTVSEAISVGNTTVTLPGVG"
BASE COUNT          384 a          923 c          978 g          476 t
ORIGIN      
        1 ggcccggcta gcaacccgag tttccagccg ccggcaccgg catgctgtgc cacgtcgaga
       61 ttgaggttcg gggcatagaa ccagccgatt ccacgcgggt tggcctcaat ggtaaaggcg
      121 gcccgcgccg gcagacgcag atcgagcttg aggcgacggt cggcggagcg ccacagatgg
      181 gcactcagcg cgggtccgat ctcgagcgtc gagcgcagat ccggcatccc cgcgcgcgcc
      241 gagtcgttac gcaccggggt cgtggcattg acgctcagat tgagctcgat ccggtcctgt
      301 ttgaagagct tgccacgcaa cccatcgcga tcggactcga ggaacttgcc gcgatagacg
      361 aagtacggca ccggcagcgg atagacgtga gtggtgtcgg ctccccggta gtcgttgaag
      421 gccagggcgc ccgctcccag tcccaattcc cacagcggct gtggctcggg gagcgccgcg
      481 gtcggcgcca gcgccatggc gctgcaggcg gccgccgcgc gtgcggctcg gcgcactctc
      541 acgcgtcggt cttgagcgga cacaggccgg tcgcctcgat ctcgaccagc aggtcctggc
      601 ggcagatgtc cgcctgcagg tagatcaccc tcgaggtggg ctcgagcgct gcggcgagct
      661 cggcgcggat gacgggcagg tctgcggggc ggcgcacata caccttgcag gcgagcgagc
      721 cgagcgagaa gcgcgccgcg ccctccacgc gattcgcctc atcgaggagc gctgcgatgt
      781 tggtgagcgt ctcgcgggtc tgggccgcgg tgtcgcccac gtgaatcgag cggtgcccga
      841 ctatgctggc ggttcccgag atgaacagcg tcaggccatc acgctggcgc agcagcgtcg
      901 cgcgcgagaa cacggggctg tggctgccgt actggcgagg gtagcggtag gcgctcagct
      961 ggcgcggatt ctcgatgaag gccggcgcgg cgcgcccggc gaggaagtac acgaccaggg
     1021 ggctgccgct cgcggctccg agcgccgagg ccgccgggac gttgccgcgg atcgcgcggc
     1081 cgcaggcact gagggcgtgc tgccgggctt gattgaactg acggtagcgc tcgatgccat
     1141 cgctgtcgcg attgatgtcc ggcaggtagt tccacacccg cagcaggtgc ggatagccga
     1201 gcgcatcgag cgtcgcgcag atctcgcggt aggcctgctc ggtggcggcg tgcagcgccg
     1261 agggggcggc gggcacgtcg ggcagggccg gggcgacctc cgactccgcg agcgcgatgc
     1321 agccgaacag caggttctcg ctgcggcgga agcggacacg gttgcggcgg ccggactgcg
     1381 ccggctggcc cgagcgccac acctcataga cggcggcggt cccgaggagc accggcgtgt
     1441 tgacctcggc cactgggatc tcgggcgcgt ccggtgacgg ggcgggcggg ccgttgaacg
     1501 aggcgacgcc gagcacgtcc gcccaccggg ccgcccggta ttcggcctgg gacaggtaat
     1561 cgaggtgcag gccggctggg gcgcgcgtca aagccacgat cgaggcgtca gttcttcggc
     1621 ttcgagtcgt cgctcttgga cgtcacagcg tccttggcct tctggccgac cgccttggcc
     1681 ccgtgcgcga tcgcatgacc ggccttcttc gagtcctcgg ccaccgcctc gcgggccggt
     1741 gcggtctttt ccgcgatctc gtgcccgacc ttgcgggagg tgtcggcgac ctcgtgcccg
     1801 actttcctgg aggtgtcggc gaccgcgtgc ccgactgtct tggcaccgtg ctcgaccgca
     1861 tgtccgaagg tcttcgcatc ctccttgacg gtctccttga cgcccgcgcc ctgggtctcc
     1921 tgagcgaggg ccgcagtcgc caagctcagg gcgagagccg tcgcgacagc agtgagggtg
     1981 aggttggctt tcatgggcaa ctatcgtacg acagtcgccg cgctgccgca cggttgggcc
     2041 ctgacttgcg gtgccaatcg tcaaggcatt cgggcacact tcgccggatg cgtctcgagc
     2101 ggctgcccat cgcggtgcta cggacccggt acgtcccgac cggctgggac gtgctcgcct
     2161 tcatcctggt gtttgctttc ttcgtgtact gcgcccaggc ggcgcacgga ctcacgggat
     2221 cgctgtcggg gctcgaggcg acgcccatct cgctcgaccc gagcgcgctc ttcggctact
     2281 cggcgcgcac ggcgctgcgc atgctgctgg cgatggtcgc ctcgctcctc ttcacgttca
     2341 cctacgcgac gctcgccgcc aagagccacc gcgccgagat cattctggtg ccgctgctgg
     2401 attttctgca gtccgtgccg atcctgagct tctcgatcac gatcctgttc tttctgcagc
     2461 tgacgcccgg gcgcgtcgcg ggggccgagc tcgcctccat ctttctcatc ttcaccagcc
     2521 aggcctggaa catggccttc agcttctacc actcgctgcg cagcatcccg gacgagctgg
     2581 tcgaggcggc gcgcaacttc cagctcggcc cctggatgcg cttctggcgg ctcgaggtgc
     2641 cgttcgccat gccggcgctc atctggaaca tgatgatgtc gatgtcgggc ggctggttct
     2701 tcgtgacggt ttcggaggcc atcagcgtcg gtaacacgac tgtgacgctc ccgggcgtgg
     2761 g
//
LOCUS       DATLKL010000624         2121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_210530, whole genome shotgun sequence.
ACCESSION   DATLKL010000624 DATLKL010000000
VERSION     DATLKL010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210530"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VM713_08430"
     CDS             complement(<1..199)
                     /locus_tag="VM713_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HVH84326.1"
                     /translation="MSPTTPRVNDFTIKIATVNGTGSASANTLLMKSIFRSGIPVVGK
                     NYFPSNIQGLPTWYEIRVTRDG"
     gene            complement(220..2037)
                     /locus_tag="VM713_08435"
     CDS             complement(220..2037)
                     /locus_tag="VM713_08435"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH84327.1"
                     /translation="MEPTDTSHPDYYHRVVDCQWACPAHTDVPEYIRLIAQGRFTDAY
                     MVNRHSNVFPGILGRVCDRPCEPACRRGRIEQKPVAICRLKRVAADHRDDIADQLPQI
                     PKVKNGKRIACIGAGCASLTVANDLMPLGYEVTILEQYGEPGGLMRTNIPSFRLPATV
                     LNEEIAMITGMGVDLKLNTPVASMRELLARGGFDAVFVGSGAPKGKELKLPGREAGSA
                     NIHIGIAWLESVAFGHVDKVGRRVLIIGVGNTAMDCCRTSLRLGAESVKVMARKPRGF
                     FKASAWELEDAEEESVEIVVNRAPKAFVIEGGRLAGMLFEKMEYELDAAGRITAERAA
                     GEEFLPADDVILAIGQENAFPWVERDLGIEFDKWDVPVIDPLTFQSTRPGVFFGGDAA
                     FGPKNIIWAVEHGHQAAISIHRHCQGEPVSERLPPGINLQSRKMGMHEWSYSNDYTPV
                     ERRLMPHVSIKERFKKLNIEVELGFTAEQIEQEVQRCLNCDMQTVFAAKLCIECDACL
                     DICPVDCLTITHNGPEGELRGRLKAPARNLAQPLYVSAALPQTGRVMVKDEDLCVHCG
                     LCAERCPTAAWDMQKSTLRWPLAADQPQSAPAQPAKKIA"
BASE COUNT          330 a          671 c          734 g          386 t
ORIGIN      
        1 agccgtcgcg ggtgacccgg atctcgtacc aggtcggcag tccctggata ttggagggaa
       61 agtagttctt gcccaccacc gggatgccgc tgcggaagat cgacttcatc agcagcgtgt
      121 tggcgctcgc cgaaccggtg ccattgaccg tggcgatctt gatggtgaaa tcgttgacgc
      181 gtggggtggt cggggacatg ggcgttcggg tccgggagtt caggcgatct tcttcgctgg
      241 ctgcgccggc gcggactgcg gctgatccgc ggcgagcggc cagcggagcg tggatttctg
      301 catgtcccag gcggcggtcg gacagcgctc ggcgcacagt ccgcagtgca cgcacaggtc
      361 ctcgtccttg accatgacac gcccggtctg cgggagcgcg gccgacacat acagcggctg
      421 cgcgagattt cttgccgggg ccttgaggcg gccgcgcagc tcgccttcgg gcccgttgtg
      481 ggtgatggtg aggcagtcca ccgggcagat gtcgaggcac gcatcgcact cgatgcagag
      541 cttcgccgcg aacaccgtct gcatgtcgca gttgaggcag cgctgcacct cctgctcgat
      601 ctgctcggcg gtgaagccga gctcgacctc gatgttgagc ttcttgaaac gctccttgat
      661 cgagacgtgc ggcatcagcc gccgctcgac gggcgtgtag tcgttcgagt acgaccactc
      721 gtgcatgccc atcttgcggc tctgcaggtt gatgccggga gggagccgct cgctcaccgg
      781 ctcgccctgg cagtgacggt ggatggagat ggccgcctga tggccgtgct cgaccgccca
      841 gatgatgttc ttaggcccaa aggcggcgtc accgccgaag aacacgcccg gccgcgtcga
      901 ctggaaggtc agcgggtcga tgaccggcac gtcccacttg tcgaactcga tgccgaggtc
      961 acgctcgacc caggggaagg cgttctcctg gccgatggcg aggatcacgt cgtcggcggg
     1021 gaggaattcc tcgcccgcgg cccgctccgc cgtgatgcgt ccggcggcat cgagctcgta
     1081 ctccatcttc tcgaacagca tgccggcaag ccggccgccc tcgatcacga aggccttcgg
     1141 cgcgcggttg acgacgatct cgacgctctc ctcctcggcg tcctcgagct cccaggccga
     1201 ggccttgaag aagccgcgcg gcttgcgcgc catgaccttg acgctctcgg cgccgagcct
     1261 gaggctggtg cggcagcagt ccatggcggt gttgccgacg ccgatgatga ggacgcgcct
     1321 gccgaccttg tcgacgtggc cgaaggcaac cgactcgagc caggcgatgc cgatatggat
     1381 gttggcgctg cccgcctcgc gccccgggag cttcagctcc ttgcccttcg gggcgccgga
     1441 gccgacgaac accgcgtcga atccgcccct ggcgagcagc tcgcgcatgc tggcgaccgg
     1501 cgtgttgagc ttaaggtcca cgcccatccc ggtgatcatc gcgatctctt cgttcagcac
     1561 cgtcgccggc aggcggaacg agggaatgtt ggtgcgcatg agcccgccgg gctcgccgta
     1621 ctgctcgagg atggtcacct cgtagcccaa gggcatgaga tcgttggcga cggtgagcga
     1681 ggcgcaaccg gcgccgatgc aggcgatgcg cttgccgttc tttaccttcg gtatctgcgg
     1741 cagttggtcc gcgatgtcat cgcggtggtc ggcggcgacg cgcttcaacc ggcagatcgc
     1801 caccggcttc tgctcaatgc gtccgcggcg gcaggcgggc tcgcaagggc ggtcgcacac
     1861 gcgcccgagg atgcccggga acacgttcga gtgccggttc accatgtagg cgtcggtgaa
     1921 gcggccctgg gcgatcaggc ggatgtactc gggaacatcg gtgtgagccg ggcaggccca
     1981 ctggcaatcc acaactcgat ggtaatagtc cggatgggag gtatcagtcg gttccattca
     2041 gtgctcgggc ctgctgacct cgagccctcg gtgcaataga agaggatggt gagtataggg
     2101 tcgcgtgacc gtttgtcatc g
//
LOCUS       DATLKL010000625         5136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_188156, whole genome shotgun sequence.
ACCESSION   DATLKL010000625 DATLKL010000000
VERSION     DATLKL010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188156"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VM713_08440"
     CDS             complement(<1..1223)
                     /locus_tag="VM713_08440"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH84328.1"
                     /translation="MRAMTPAAAINSSPVEREVARIRALLKERRYPEALRASGTLLST
                     VPENRDVLYLHAVAQRYLGEIGAALTTLAELERLHPRFSGLYQERGNCYVALKQAPQA
                     IDAFLRGVTINPALPASWSMLEGLFRMTGQDTQAAQAAAHLAALKKLPPEVVTATALF
                     ADGELGAAEHLIRAFLLQHGHHVEAMRLLARLGIEREVLDDAQLLLEGVLELAPDYQA
                     ARADYVRVLTERHLYAQAQEQAEKLLAADPANRNYRTIHALTCVGLGQHERAIGLYRE
                     LLDGAAQPADLHLSIAHSLKTLGRSTEAVAEYRAAAAARRDFGDAYWSLANLKTYRFE
                     DAEVAAMQAAEGAPATAAVDRYHLCFALGKALEDRGQYAQSFAYYERGNALRRAASRS
                     RPEVHELNTRLQIEV"
     gene            complement(1264..4059)
                     /locus_tag="VM713_08445"
     CDS             complement(1264..4059)
                     /locus_tag="VM713_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVH84329.1"
                     /translation="MPIKRPALAHRHRSQISCAVAAILGGASLAAQAQVQPPATGAQA
                     SETSGNALAEITVTAQRRTENIQDVPISIQALTAQALQQLNVSTFDDYLRYLPNVTSA
                     SNGPGQSEVFMRGLSAGSQASQGSGSTAAWPNVAIYLDNQSGQLPNRNLDIYAADLNR
                     IEVLEGPQGTLFGAGAEAGAIRYITNEPKLENTEANIKAGYGTTAHGDHNTDVTAVLN
                     LPVIPGTMALRAVIYDDRRGGYIDNVPGTFTRKNTDIGIHYADYATACSAGTPIAGIC
                     PGGNATATAFGVPPGSPSLTNAGLIGNAINPVTYEGIRVEGLYKFNDGWDLLISQSYQ
                     DMHSLGVFYQQPNASDGAPLAPLQVTLFNPAFDTDRFESTAWTVNGRIADLKLVYTGG
                     YLVRNVDQAGDYTNYARGVFADYYQCYGPPGAGTFVIDPSLTPTCFSPRATWRETERN
                     THQQHELRLSTPDDWRLRAIGGVYWEDNKLYDQTRWNYKTMPPCTTNATPGTPGNGNT
                     GCLSNVGTFAGTTVVNPGVQSDNTSFYQDQVRETKQLAEFISLDYDLIPKVLTLTGGT
                     RHFKFDNSMAGSVLSSFGCFEGGVPPGGCQTLTAALPSSYNLDAQNLKDTETGFKSRG
                     NLTWHVTPDAMVYYTFSQGFRPGGFNQNHMAADGSVFKYAPGPDGINQFAVPRSYLSD
                     KLTNNEIGWKTEWLDHRLQWNGALYREEWDNVQVAFFDPGVTGNVFFDTNGQNFLIKG
                     VETSLVARVVTGLTLQAAGSWNRSEQTNSPTLIDLNPASVNYGKAITQVCSTTGTNCA
                     PIVNPYGPIGSPSANSPPLQFSARIRYDWNVSGYTPFIQFGATHSGHSFTQAGANPTF
                     GLGETVSNSRGRFENPAYTMYDASAGIAKDAWYANVFIENLSNSNASVFVSTDQFIVE
                     QTPLRPRVIGLTVGYSF"
     gene            complement(4141..>5136)
                     /locus_tag="VM713_08450"
     CDS             complement(4141..>5136)
                     /locus_tag="VM713_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HVH84330.1"
                     /translation="PLRVGVILAHHFTLSASAVFIDHLRLAADEGDRSRPLHVQWSIM
                     ASRPESVPASCGVSLEPTSGFLTPDSLDYVVVIGGILHAGPQVDEATMRYLREVGSTN
                     VPLIGICTGSFVLCRAGLMKGRRSCVSWYHYQDFLEAFPTHEVIADRLFLVDGLRITC
                     AGGAGAAALATFLIEQHLGRAVAQKASQVLLFDRPRSGSEAQPHPPMSEVVSEPRVRR
                     ALLLMEQNLARPIAIATIAAELGLSARQLERLCRQHVGMGPASLYRQLRMRYANWLIE
                     NTDRSVTDIANEAGFADCAHFSRQFKDAYGLSPSTRRLQPQRAANGDAARARVFE"
BASE COUNT          816 a         1583 c         1829 g          908 t
ORIGIN      
        1 cagacttcga tctgcagccg ggtgttgagc tcgtgcacct cgggccggga gcggctggcg
       61 gcgcggcgca gcgcattgcc gcgctcgtag tacgcgaagg actgcgcgta ctggccgcgg
      121 tcctcgagcg ccttgcccag cgcgaaacac agatggtagc ggtcgaccgc cgcggtcgcc
      181 ggagcgcctt ccgcagcctg catcgcggcg acctccgcat cctcgaagcg ataggtcttg
      241 agattggcga ggctccagta ggcatcgccg aagtcgcggc gggccgccgc cgcggcgcga
      301 tactccgcaa cggcttccgt gctgcgcccg agcgtcttga gggaatgcgc gatcgagaga
      361 tgcagatccg cgggctgcgc agcgccgtcg aggagctcgc gatagagccc gatggcacgc
      421 tcgtgctggc cgaggccgac gcaggtcagc gcatggatcg tccggtagtt gcgattggcg
      481 ggatcggcgg cgaggagctt ctctgcctgc tcctgagcct gggcgtagag gtggcgctcc
      541 gtgaggacac gcacgtaatc cgcgcgcgcc gcctgatagt caggagcgag ctcgagcacg
      601 ccctcgagca gcagctgcgc atcatcgagc acctcgcgct cgatcccaag acgcgcgagc
      661 agccgcatgg cctcgacgtg atggccgtgt tgcaggagga aggcgcgaat caggtgctcg
      721 gccgccccga gctcgccgtc cgcgaacagc gccgtcgccg tgactacctc gggcggcaac
      781 ttcttcagcg ccgccagatg cgcggcggct tgcgcggcct gggtatcctg tcccgtcata
      841 cggaacagtc cctcgagcat gctccagctc gctggcagcg ccggattgat ggtgacgccg
      901 cggaggaagg cgtcgatggc ctgcggtgcc tgcttcaacg ccacgtagca gttgccgcgc
      961 tcctggtaga ggccgctgaa gcgcggatgg agccgctcga gctccgcgag cgtcgtgagc
     1021 gccgcaccga tctcgccgag gtagcgctgc gccaccgcgt ggagatacag cacgtcgcgg
     1081 ttctcgggca cggtactgag cagcgtgccg ctcgcgcgca gcgcctcggg gtagcgacgc
     1141 tccttgagca gggcgcggat gcgcgcgacc tcgcgctcga ccggggagga gttgatcgcg
     1201 gcagccggtg tcatggcgcg catgatgcgc ccggccgccg cgggctggct atgagcttcg
     1261 ccgttagaag ctgtatccga cggtgagacc gatcacccgc gggcgcagcg gcgtctgctc
     1321 gacgatgaac tgatcggtgc tgacgaacac gctggcgttc gagttgctca ggttctcgat
     1381 gaacacgttg gcgtaccagg cgtccttggc gatcccggcg gaggcgtcgt acatggtgta
     1441 ggcgggattt tcgaaccggc ctctggagtt cgagaccgtc tctccgagtc cgaaggtcgg
     1501 attcgctccg gcctgggtga aggaatggcc gctgtgcgtg gcgccgaact gaatgaaggg
     1561 cgtgtagccg ctgacgttcc agtcataacg gatgcgcgcg ctgaactgca gcggcggcga
     1621 gttggcgctc ggcgagccga tcggcccgta cgggttgacg atcggcgcgc agttagtgcc
     1681 ggtggtgctg cagacctgag tgatcgcctt gccgtagttg acgctcgccg gattgagatc
     1741 gatgagggtc ggggagttgg tctgctcgct gcggttccac gagcccgccg cctgcagcgt
     1801 gaggccggta acgacccggg caacgagcga ggtctcgacg cccttgatga gaaagttctg
     1861 cccgttggtg tcgaagaaga cgttgccggt cacaccggga tcgaagaagg cgacctgcac
     1921 gttgtcccac tcctcgcggt agagagcccc gttccactgc aggcgatgat cgagccactc
     1981 ggtcttccag ccgatctcgt tgttggtaag cttgtcggat aaataggacc tcggtacggc
     2041 gaactggttg atgccgtccg gccccggcgc gtacttgaat actgagccgt cggcagccat
     2101 gtgattctgg ttgaacccgc ctgggcgaaa gccctgtgag aaggtgtagt acaccatggc
     2161 gtcgggcgtc acgtgccaag tgaggttgcc gcggctcttg aagcccgtct cagtgtcctt
     2221 caggttctgg gcgtcgaggt tgtaagagga cggcaatgcg gcagtaagcg tctggcatcc
     2281 accgggcggc acgccgccct cgaagcagcc gaagctcgag agcacgctgc ctgccatcga
     2341 attgtcgaat ttgaagtgcc gtgtgccgcc ggtgagcgtg agtaccttcg ggatgaggtc
     2401 gtagtcgagc gagatgaact ccgccagctg cttggtctcg cgcacctgat cctgatagaa
     2461 ggaggtgttg tcgctctgca cccccgggtt cactaccgta gtgcccgcga aggtgccgac
     2521 gttcgagagg cagccggtgt tgccattgcc gggggtgccg ggggtagcgt tagtggtgca
     2581 cggcggcatg gtcttatagt tccagcgcgt ttgatcgtag agcttgttgt cttcccagta
     2641 gacgccgccg atcgcgcgca gccgccagtc gtccggtgta ctcaggcgca gctcatgctg
     2701 ctgatgcgtg ttgcgctcgg tctcccgcca ggtggccctc ggcgagaagc aggtcggggt
     2761 gagcgacgga tcgatcacga acgtcccggc tccgggcggg ccgtagcact ggtagtagtc
     2821 ggcgaacacg ccgcgggcgt agttggtgta gtcgcccgcc tgatcgacgt tgcgcaccag
     2881 atagccgccc gtgtagacca gcttgagatc ggcgatcctg ccgttcaccg tccaggcggt
     2941 gctctcgaag cggtcggtgt cgaaggcggg attgaacagc gtgacttgca gcggcgcgag
     3001 cggcgcgcca tccgaggcat tcggctgctg gtagaagacc ccgagcgagt gcatgtcctg
     3061 gtaggactgg ctgatgagca gatcccagcc gtcgttgaac ttgtagaggc cctcgacgcg
     3121 gattccctcg tacgtgacgg ggttgatggc gttgccgatg aggccggcgt tggtgagcga
     3181 ggggctcccc ggcggcacgc cgaaggcggt cgccgtcgcg ttgccgccgg gacaaatgcc
     3241 ggcgatcggc gtgcctgccg agcaagccgt ggcgtagtcg gcgtaatgaa tgccgatatc
     3301 ggtgttcttg cgcgtgaatg tccccgggac gttgtcgatg tagccgccac ggcgatcgtc
     3361 gtaaatcacc gcgcgcaagg ccatggtgcc gggaatcacc gggaggttca gcaccgccgt
     3421 cacgtcggta ttgtgatcgc catgggcggt ggtgccgtag ccggccttga tgttggcctc
     3481 ggtgttctcc agcttcggct cgttggtgat gtagcggatg gcgcccgcct cggcgccggc
     3541 gccgaacagc gtcccctgcg ggccctccag cacctcgatg cggttgagat ccgcagcgta
     3601 gatgtcgagg ttgcggttgg gcagctgtcc cgactggttg tcgaggtaga tggcgacgtt
     3661 cggccacgcg gcggtcgagc cgctgccctg gctcgcctgc gagccggcgc tcaagccgcg
     3721 catgaagact tcgctctgcc cgggaccgtt gctcgccgag gttacgttcg gcagataacg
     3781 caggtagtca tcgaaggtcg agacgttgag ctgttgcagt gcctgcgcag tgagtgcctg
     3841 aatcgagatc ggcacgtcct ggatgttctc cgtgcgccgc tgtgccgtca cggtgatctc
     3901 tgccagcgca ttgccactcg tctcgctagc ctgggcaccc gtggcggggg gctgcacctg
     3961 cgcttgcgcc gcgagcgatg cgccgccgag gatggccgct acggcacagg agatctgcga
     4021 gcgatgacgg tgggcgaggg cagggcgctt gattggcatg gtcgactccc ctggcgacgt
     4081 caggccggct ggattagagc ctagcggccg ggcctgcgat tgaacggaca cgtcaaactg
     4141 ttattcgaaa actctggcac gcgcggcatc gccattcgcg gcgcgctgcg gctgcaggcg
     4201 ccgggtcgag ggcgacaggc cgtaggcatc cttgaactgg cgcgaaaaat gcgcgcagtc
     4261 ggcaaacccg gcctcgttgg cgatgtcggt aaccgatcga tcggtgtttt cgatgagcca
     4321 gttggcatag cgcatgcgca gctggcggta gagcgacgcc ggccccatgc cgacgtgctg
     4381 ccggcacagc cgctcgagct gccgggccga gagtcccagc tccgcggcga tggttgcgat
     4441 ggcgatgggc cgcgcgagat tctgctccat gagcagcagc gcgcgacgta cccgcggctc
     4501 gctcactact tccgacatag gcggatgggg ctgagcctcg ctgcccgagc gcggccggtc
     4561 gaacagcagt acctggctgg ccttctgcgc taccgcgcgt cccaggtgct gctcgatgag
     4621 gaacgtggcg agcgcagccg cgcccgcgcc gccggcgcag gtgatgcgca gtccgtcgac
     4681 caggaacagc cggtcggcga tgacctcgtg cgtgggaaag gcctcgagaa aatcctggta
     4741 gtggtaccag ctgacgcatg agcggcgccc cttcatcagg cccgcacggc acagcacgaa
     4801 gctgcccgta cagatgccga tgagcggcac gttggtcgag ccgacctcgc gcagatagcg
     4861 catggtcgcc tcatccacct gcggtccggc gtgcaggatg ccgccgatca ccaccacgta
     4921 atcgagggag tccggcgtca ggaagccgct ggtgggctca agcgagacgc cgcagctcgc
     4981 cggcaccgac tcgggccggc tcgccatgat cgaccattgc acgtgcagcg ggcggctgcg
     5041 gtcgccttca tccgcggcca gccgcaggtg atcgatgaag accgcggagg cggacagcgt
     5101 gaagtggtgc gcgaggatga ctccgacccg caatgg
//
LOCUS       DATLKL010000626         2870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_188186, whole genome shotgun sequence.
ACCESSION   DATLKL010000626 DATLKL010000000
VERSION     DATLKL010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188186"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VM713_08455"
     CDS             <1..342
                     /locus_tag="VM713_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVH84331.1"
                     /translation="VYDAVGRYRFMTSLDCMRRRTMMVTFGNASGPVPAIAPLELSKR
                     GSLFLTRPTLFHYIGTRPELEAAARELFAAVRARKVRVVIGQRYPLRQAADAHRDLEG
                     RRTTGSTVLTP"
     gene            complement(440..958)
                     /gene="ilvN"
                     /locus_tag="VM713_08460"
     CDS             complement(440..958)
                     /gene="ilvN"
                     /locus_tag="VM713_08460"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HVH84332.1"
                     /translation="MRHIIAILLQNEAGALTRVAGLFSTRGYNIESLSVAPTDDPTVS
                     RLTLVTSGSDAVIQQIVNQLNKLVDVVNVLDMTRGQHLERELVLLKLRVEPQHTDTLR
                     GLVVRSGGRVLDPATEGFIVELTASEAEINAFIADLARGAQLLEVVRSGALGIPRTAR
                     ALRAAAPATADA"
     gene            1116..>2870
                     /gene="ilvB"
                     /locus_tag="VM713_08465"
     CDS             1116..>2870
                     /gene="ilvB"
                     /locus_tag="VM713_08465"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HVH84333.1"
                     /translation="MNAPSPSDPDADLRPAAHPLAGTRMSGARMIMQVLADEGVTAIF
                     GYSGGAILPTYDAVFLYNEECARAGRPQIPLIVPANEQGAGFMAAGYARASGRVGVCI
                     VTSGPGATNTVTPVRDCMADSVPIVVICGQVPRAAIGTDAFQEAPVPAVMGAVAKHIF
                     LVTEPEKLEATVRTAFEIARTGRPGPVVVDIPKDVQNWEGTFQGAGLLPIPGYRRRLD
                     ELTRAALDPAAAGRFFAMLGEAQRPLIYAGGGVINGNAAARLTEFTAAFGVPVVTTLM
                     GIGSLDTRTALSLRMLGMHGAAFANYAVDDCDFLIAVGARFDDRVAGVPEKFARNARR
                     IAHLDVDRAEVNKVKRVQWSHVGLVAEALAALTAHGRQAGFQRDFAAWLAELAELKRR
                     YAMNYDRASPLIQPYYVLEELNRHTRGEAIISTGVGQHQMWAAQYCDFAGPRLWLTSG
                     SMGTMGFGLPAAIGAQIAQPQRLVIDIDGDSSIRMNIGELETATTYDLPVKVMVLNNF
                     GDGMVKQWQKLFFKGRLSASDRSLHKKDFVRAAQADGFRYAVRLEHKADVPRVIAEFV
                     GFKGPAFLEVMIDPDAGVY"
BASE COUNT          443 a          995 c          994 g          438 t
ORIGIN      
        1 gtctatgacg cggtcggccg ctacaggttc atgacatcgc tcgactgcat gcgccgccgc
       61 acgatgatgg tcaccttcgg caatgcctca ggtcccgtac cggcgatcgc gccgctcgag
      121 ctcagcaagc gcggctcgct cttcctgacg cgcccaacgc tattccacta catcggcacg
      181 cgccccgagc tcgaggcggc ggcgcgcgag ctgttcgccg cggtgcgcgc gcgcaaggtg
      241 cgcgtcgtga tcgggcagag atatccgctg cggcaggcgg ccgatgcaca ccgcgacctc
      301 gagggccggc gcaccaccgg ctcgacggtg ctgacgccgt agccgctcta gcggcgcagc
      361 cggctcgcgg ccagcgcgac cggccccacc gcactcagga gccccgtgtc gcgctggctc
      421 gcccgcaacc ggtcgcgcct tacgcgtccg cggtcgcggg cgctgcggcc cgcagggcgc
      481 gcgccgtgcg cggaatgccg agcgcgccgc tgcgcaccac ctcgaggagc tgcgcgccgc
      541 gggccaggtc cgcgatgaaa gcgttgatct ctgcctcgct tgcggtcagc tcgacgatga
      601 agccctcggt ggccggatcc agcacgcggc cgccgctgcg cacgacgagt ccgcgcagcg
      661 tatcggtgtg ctgcggctcg acccgaagct tcagcagcac cagctcccgc tcgaggtgct
      721 ggccgcgggt catgtcgagt acgttcacga cgtccaccag cttgttcagc tgattgacga
      781 tctgctggat gactgcgtcc gagccgctgg tgacgagcgt caggcgcgag accgtgggat
      841 cgtcggtggg ggccaccgac agcgattcga tgttgtagcc gcgggtggaa aagagccccg
      901 ccacgcgggt gagggcgccc gcctcgttct gcagcagtat ggcaatgatg tggcgcatgt
      961 tttgggcgcg cggcgctgac ggggaagggc ctcggtggca gaaaaaccct cggcagaata
     1021 gaaggatcgg cgcgagtttg caccacgaac ccaagctgtt acgctcaaaa gttctgtcgc
     1081 tgtcccagcc ccaagaggtt tcccaaaacc cgccgatgaa cgccccctcc ccgagcgacc
     1141 ccgacgccga cctccgaccg gcggcccatc cgctcgccgg gacgcggatg agtggcgcgc
     1201 gcatgatcat gcaggtgctc gccgacgagg gggtgacggc gatcttcggc tacagcggcg
     1261 gggcaatcct cccgacctac gatgccgtct tcctctacaa cgaggagtgc gcgagggccg
     1321 gccggccgca gattcccctg atcgtccccg ccaacgagca gggagccggg ttcatggcgg
     1381 cgggctacgc ccgcgccagc gggcgggtcg gcgtctgtat cgtgacttcg gggccggggg
     1441 cgaccaacac tgtcacaccc gtgcgcgatt gcatggccga ctctgtgccg attgtcgtga
     1501 tctgcgggca agtgccgcgg gcggcgatcg ggacggacgc gttccaggaa gcgccggtgc
     1561 ctgccgtcat gggcgccgtt gccaagcaca tttttctcgt taccgagccg gagaagctcg
     1621 aggcgaccgt gcgcacggcc ttcgagatcg cccgcaccgg ccgcccggga cccgtggtgg
     1681 tcgatatccc gaaggacgtc cagaactggg aggggacttt ccagggagca ggactgctgc
     1741 cgatccccgg ctaccggcgg cggctcgacg agctcacgcg cgccgcgctc gatccggccg
     1801 cggccgggcg cttctttgcc atgctgggcg aggcgcagcg cccgctcatc tacgccggcg
     1861 gcggggtcat caacggtaac gccgcggcgc gactcaccga gttcaccgcg gccttcggcg
     1921 tgccggtggt gaccacccta atgggcatcg gctcgctcga cacgcgcacg gcgctgtcgc
     1981 tgcggatgct cgggatgcac ggcgccgcct tcgccaacta cgccgtcgac gactgcgact
     2041 tcctcatcgc cgtcggggcg cgcttcgatg accgcgtcgc gggggtgccc gagaagttcg
     2101 cccgcaacgc caggcgcatc gcgcatctcg acgtggaccg cgccgaggtc aacaaggtga
     2161 agcgggtgca gtggagtcac gtgggcctgg tggcggaggc gctcgcggcg ctcaccgcgc
     2221 acgggcggca ggcgggattc cagcgggact ttgctgcctg gctcgccgag ctcgccgagc
     2281 tcaagcgccg ctacgccatg aactacgacc gcgcgagccc gctgatccag ccgtactacg
     2341 ttctcgagga gctcaaccgc cacacccgcg gcgaggcgat catctcgacc ggcgttggcc
     2401 agcaccagat gtgggcggcg cagtactgcg atttcgccgg cccgcgcctg tggctcacct
     2461 ccggcagcat gggcacgatg ggcttcgggc tcccggcggc gatcggcgcg cagatcgcgc
     2521 aaccgcagcg cctggtgatc gacatcgacg gcgactcgag catccgcatg aacatcggcg
     2581 agctcgagac cgcgaccacc tacgacctgc cggtgaaggt catggtgctc aacaatttcg
     2641 gcgacgggat ggtcaagcag tggcagaagc tgttcttcaa ggggcggctg tcggcgagcg
     2701 acaggtcgct ccacaagaag gacttcgtcc gcgccgcgca ggccgacggc ttccgctacg
     2761 cggtgcgact cgagcacaag gcggacgtgc cgcgcgtcat cgctgagttc gtcggcttca
     2821 aggggccagc ctttctcgag gtgatgatcg accccgatgc cggcgtctat
//
LOCUS       DATLKL010000627         1712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_131844, whole genome shotgun sequence.
ACCESSION   DATLKL010000627 DATLKL010000000
VERSION     DATLKL010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131844"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VM713_08470"
     CDS             <1..304
                     /locus_tag="VM713_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HVH84334.1"
                     /translation="FIKPLDEDLLVALARRHRALVTIEENVVHGGAGSAVGELLAAEG
                     LLTPLLQLGIPDRFIEHGSREGCLAAAGLDAASLRASIERWWSLHGQERLRSAGGT"
     gene            397..1221
                     /gene="folE2"
                     /locus_tag="VM713_08475"
     CDS             397..1221
                     /gene="folE2"
                     /locus_tag="VM713_08475"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HVH84335.1"
                     /translation="MTPPAATAKIVRAPLAEVEDVQARADTRRLPINRVGIKDISHPV
                     RVRDRSAGEQHTIATFNMYVSLPHNFKGTHMSRFVEALHAEREISVESFSAMLEGMTA
                     RLEAETGHIEMSFPFFVMKKAPVSQVESLMDYRASLIGERRHGRTELWVRVAVPVTSL
                     CPCSKRISDYGAHNQRSHVTITAKLRSHMWIEELIEIAESEASCELYGILKRPDEKYV
                     TERAYDNPKFVEDMVRDVAGRLNRDERVAAYVVESENFESIHNHSAYALIEHDKDA"
     gene            1364..1609
                     /locus_tag="VM713_08480"
     CDS             1364..1609
                     /locus_tag="VM713_08480"
                     /inference="COORDINATES: protein motif:HMM:NF019389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HVH84336.1"
                     /translation="MNWKILIAALGLIAGGTTLAADPPAAPSATPVKHPSLKSCNKEA
                     TARQLSGRQREQFVKDCTAGKAHAAPPPTAQQPSATH"
BASE COUNT          308 a          606 c          549 g          249 t
ORIGIN      
        1 cttcatcaaa ccgcttgacg aggacctgct cgtcgcgctc gcgcggcgcc accgggcgct
       61 ggtgaccatc gaggagaacg tcgtgcacgg cggcgccggg tccgcggtcg gggagctcct
      121 cgccgcggag ggactcctca ccccgctgct ccagctcggc atccccgacc gtttcatcga
      181 gcacggctcg cgcgaaggat gcctcgcggc cgccgggctc gatgcggcct ctctgcgcgc
      241 cagcatcgag cgctggtggt cgctgcacgg ccaggaacgc ctgcgttcgg ccgggggaac
      301 ctgagctcct cacaaagaaa gaggcggacg gaggcgcgtc gcggccatcc taggcgccga
      361 ggcagcgtcg ctgcgcattc ctgtaggctc tgacacatga caccccctgc cgccaccgcc
      421 aagatcgtcc gtgctcccct cgccgaagtc gaggacgtgc aggcgcgcgc cgacacgcgc
      481 cgcctgccga tcaaccgcgt cggcatcaag gacatcagcc accccgtgcg ggtcagggat
      541 cgctcggccg gcgagcagca caccatcgcc accttcaaca tgtacgtgag cctgccgcac
      601 aacttcaagg gcacgcacat gtcgcgcttc gtcgaggcgc tgcacgccga gcgcgagatc
      661 tcggtcgagt ccttcagcgc catgctcgag gggatgacgg cgcggctcga ggccgagacc
      721 ggccacatcg agatgagctt cccgttcttc gtgatgaaga aggcgccggt atcgcaggtc
      781 gagagcctga tggattaccg cgcaagcctc atcggcgagc gccgccacgg gcgcacggag
      841 ctgtgggtgc gcgtcgcagt tccggtgacg agtctctgcc cctgctcgaa gcgcatctcc
      901 gactacggcg cgcataacca gcgctcgcac gtcaccatca ccgccaagct ccgcagccac
      961 atgtggatcg aggagttgat cgagatcgcc gagagcgaag cctcgtgcga gctctacggg
     1021 atcctcaagc gtccggacga gaagtatgtg accgagcgcg cttacgacaa tccgaaattc
     1081 gtcgaggaca tggtgcgcga tgtggcgggg cggctcaatc gcgacgagcg cgtcgccgcc
     1141 tacgtggtcg agtcggagaa cttcgagtcg atccacaacc actcggccta cgcgctcatc
     1201 gagcacgaca aggacgcctg agaatcgcgc gtcgtcggcg cccggggcgc cgccacagcg
     1261 ctgcatgacg atttcttaat gtcgccgtca tcggcgcgac agggcccggc ggctaagttc
     1321 ttccatcccc gcggtaggct ttcggcagcg aggagctgtg cgcatgaact ggaagatcct
     1381 gatcgccgct ttaggcctca tcgccggcgg caccacgctg gccgccgacc cgccggccgc
     1441 accatccgct acgcccgtga agcacccgag cctcaagtcc tgcaacaagg aagcaaccgc
     1501 aagacagctc tcgggccggc agcgcgagca gttcgtcaag gactgcacgg ccgggaaggc
     1561 tcacgccgca ccgccgccga cggcgcagca accgtccgcc acgcactagc gcaaacccgc
     1621 ccattgacta gcgggcaaaa gcccgtgcgc cagcctcaat acgaagtgct cggctgctga
     1681 ggtgagcgtg gcagctgcgc atcgcgcccc gc
//
LOCUS       DATLKL010000628         2163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_41498, whole genome shotgun sequence.
ACCESSION   DATLKL010000628 DATLKL010000000
VERSION     DATLKL010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41498"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VM713_08485"
     CDS             complement(<1..1156)
                     /locus_tag="VM713_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVH84337.1"
                     /translation="MHIRPLLAAAATLLAGCAMGPEFRRPNPPAAVEYTADGQPESTV
                     AADGEAGAAQRFVTGAELPARWWESFHCEALNTLVADALTHSPTARQARARLREARED
                     LAAQGGSTLYPSLDAQLGATRERVNPAAFGFSNIPPTPPLSLYNAQVNVSYTLDLLGG
                     NRRMLEGVRAQTEYQAYETESAELTLAANIVAAAIHEADLAAQVQYTEHLITAQTRQL
                     AISEERYQAGGVALEDLDTQRSQLEQLRATLPLLTAQQREVEHLLAVYSGKVPSAAGA
                     MRFRLSDLSLPADVPVTLPSELVRRRPDVRASEALWHEASANVGLASANLFPHLTLSG
                     YAGSQRSQASQLVDGTNVWSVGASLLQPIFHGGELRARQRSAVAAYEVAAA"
     gene            complement(1170..>2163)
                     /locus_tag="VM713_08490"
     CDS             complement(1170..>2163)
                     /locus_tag="VM713_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVH84338.1"
                     /translation="DPKHLLTGVLIEDHSQFTRTFVAGMKASNYFDIVAELPDEQAAR
                     AALARGALQFVLTIPPDFSRRLLRGERPALLLEADATDPSATGNAIAAALRLPQSVAA
                     KDLIGPLAPLAGGQPPFEVRLHSLYNPEGITQYFIVPGIMGVILSMTLVLMTGLAITR
                     ERVRGTMENLLAMPVTPLEVLSGKMVPYIFIGLIQVSIILLAAALMFHVPFEGSLLTF
                     YCAALFFVAGNLTVGISLSSLAQNQLQAMQLTMFYFLPNILLSGFMFPFQGMPRWARY
                     IGEVLPLTHFNRLVRGIMLKGNGWPDLWTDVWPIGLFIMLVMFIALRFYRRTLD"
BASE COUNT          367 a          666 c          805 g          325 t
ORIGIN      
        1 ccgcggcggc gacttcgtag gcggcgaccg cggagcgctg ccgcgcgcgc agctcgccac
       61 cgtggaagat cggttgcagc aggctcgcgc cgacgctcca gacgttggtg ccgtcgacga
      121 gttgcgaggc ctgcgagcgc tgcgagccgg cgtaacccga gagcgtgagg tggggaaaga
      181 ggttcgcgct ggccagaccg acattggcgg acgcctcgtg ccatagagcc tcggatgcgc
      241 ggacgtcggg ccgccgccgc acgagctcgg atggcaacgt cacgggcacg tcggcgggca
      301 ggctcagatc gctcagccgg aagcgcatgg cgccggctgc ggacggcacc tttccactgt
      361 agacggcgag caggtgctcg acctcccgtt gctgcgccgt gagcagcggt agcgtggcgc
      421 gcagctgctc gagctgactg cgctgcgtgt cgaggtcctc gagtgcgacg cccccggcct
      481 ggtagcgctc ctcgctgatc gcgagctggc gggtctgggc ggtgatcaaa tgctccgtgt
      541 actgcacctg ggccgcgagg tctgcctcat ggattgctgc agcgacgata ttggccgcca
      601 gagtcagctc ggccgactcg gtttcgtagg cctggtactc ggtctgcgcg cggactccct
      661 ccagcatgcg gcggttgccg ccgaggaggt cgagtgtgta cgagacattg acctgggcgt
      721 tgtagaggct gagcggtggc gtcggcggaa tgttcgagaa gccgaatgcc gccgggttga
      781 ccctctcgcg cgtggcgccg agctgcgcat cgagcgacgg gtagagcgtc gagccgccct
      841 gagcggcgag atcctcgcgc gcctcgcgca gccgcgcgcg cgcctgccgc gcggtgggac
      901 tgtgcgtgag cgcatccgcg acgagggtat tcagcgcctc gcaatgaaac gactcccacc
      961 aacgggccgg gagctcggcg cccgttacga agcgctgcgc ggcgccagcc tcgccgtcgg
     1021 cggcgaccgt cgattcgggc tgaccgtcgg ccgtgtactc aacggcagcg ggagggttcg
     1081 gacggcggaa ctccgggccc atggcacatc cggcaaggag cgtcgccgcg gcggcgagaa
     1141 gagggcggat gtgcatgtgg ctgcctccgt cagtcgagcg tccggcggta gaaccgcaac
     1201 gcgatgaaca tgaccagcat gatgaagagt ccgatgggcc agacatcggt ccacagatcc
     1261 ggccagccgt tgcccttcag catgatgccg cgcaccaggc ggttgaagtg cgtgaggggc
     1321 aggacctcgc cgatgtagcg ggcccagcgc ggcatgccct ggaacgggaa catgaagccg
     1381 gacagcagga tgttgggcag gaagtagaac atcgtgagct gcatcgcctg cagctggttc
     1441 tgcgcgagcg acgagagcga gatcccgacg gtgagattgc cggcgacgaa gaagagcgca
     1501 gcgcagtaga aggtgaggag actgccctcg aagggcacgt gaaacatcag cgccgctgcc
     1561 agcaggatga tgctgacctg gatcaagcca atgaagatat acgggaccat cttgccgctc
     1621 agcacctcga gcggagtcac gggcattgcc aggaggttct ccatggttcc gcgcacgcgc
     1681 tcacgggtga tcgcgagccc cgtcatgagg accagcgtca tgctgaggat cacacccatg
     1741 atgccgggca cgatgaagta ctgggtgatg ccctcggggt tatagagact atggagacgc
     1801 acctcgaagg gcggctggcc gccggcgagc ggcgcgagcg gtccgatgag atccttggcc
     1861 gccaccgact ggggcagcct gagtgccgcg gcgatcgcgt tgccggtggc ggacgggtcg
     1921 gtggcgtccg cctcgaggag cagcgccggg cgctcgccgc gcagcaggcg gcggctgaaa
     1981 tcgggcggaa tggtcaggac gaactgcagc gcgccacggg cgagcgccgc acgggcggcc
     2041 tgctcgtcgg gcagctccgc gacgatgtcg aaataattcg aggccttcat cccggcgacg
     2101 aaggtgcggg tgaactggct gtgatcctcg atcagcactc ccgtcaggag gtgcttgggg
     2161 tcg
//
LOCUS       DATLKL010000629         1677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_120504, whole genome shotgun sequence.
ACCESSION   DATLKL010000629 DATLKL010000000
VERSION     DATLKL010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120504"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..761
                     /locus_tag="VM713_08495"
     CDS             144..761
                     /locus_tag="VM713_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84339.1"
                     /translation="MLRVLLLSASLCALWVTVPCSAVPAPPIVMPPSSPAPPPGAAPP
                     APSPGCLPTGNGYLRARIRGALNLDIDWRNGELECDGGPRPDGSGIRMSFAGPALPGG
                     RRLRLVFGVGAVREGRSGRSLPTNLTLIVEGGQRLYATRGDDHCTVDTLNQQRLRAPG
                     APRRYRVVARGFCVAPAMTLNNTERILISSFDFAGRAVFGDDAVR"
     gene            772..1272
                     /locus_tag="VM713_08500"
     CDS             772..1272
                     /locus_tag="VM713_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HVH84340.1"
                     /translation="MKTNALRAAGTALAVALASAVHADPPLLDLATFPRAALEIVQPV
                     PQPARRFHFDVWVADTPARAEQGLMFVRDLPAGRGMVFPFEPARVATFWMRNTYIELD
                     LLFIAADGRVAKIIERAAPLRDDRLSSDVRVGAVLELRGGEVGLLGLRVGDQVSWKPA
                     GKASAP"
     gene            complement(1277..>1677)
                     /locus_tag="VM713_08505"
     CDS             complement(1277..>1677)
                     /locus_tag="VM713_08505"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HVH84341.1"
                     /translation="AEPLRAYLGGIGRGSDHPFSGRNQGDAQIASAWSVRLGRGGFHV
                     NHFHNHGWISSAYYVEVPEEAGDAELKSGWLRLGEPRFPTPGAGVGCVVQPHAGQLVL
                     FPSYMWHGTSPILGSQPRTAVSFDAVPAGG"
BASE COUNT          223 a          608 c          592 g          254 t
ORIGIN      
        1 tgctgctcgc tctcggtgtc gcgctcctca cgatgctcct gcacctggcg cgcggcttgg
       61 tccgggtgca cgcgcggctc gcgaaggcga tgttggtcgt gcccggggcg taaccggcgc
      121 cacgccacgt tacactccgg cgcatgctgc gggttcttct cctgagcgcg tcgctgtgcg
      181 cattgtgggt gaccgtaccc tgctcggcgg tgccggcacc gccgatcgtg atgccgccga
      241 gcagccctgc gccaccgccg ggcgccgcgc cgccggcccc atcacccggc tgcctgccga
      301 ccggcaacgg ctatctcagg gcgcgcatcc gcggcgcact caatctggac atcgactggc
      361 gcaacggcga gctcgaatgc gacggcggac cgcggccgga tggcagcggc atccgcatga
      421 gcttcgcggg gcctgcgctc cccggcggtc ggcggctgcg cctcgtgttc ggggtgggcg
      481 cggtgcgcga gggccgcagc ggccgcagct tgccgaccaa cctcaccctg atcgtcgagg
      541 gcggccagcg cctctacgcg acccgtggcg acgaccattg cacggtggac acgctcaatc
      601 agcagcgctt gcgcgcgccg ggcgcgccgc gcaggtatcg ggtcgtcgcg cgcggcttct
      661 gtgtggcccc ggcaatgacg ctcaacaaca ccgagcgcat tctcatcagc agcttcgatt
      721 tcgcgggccg cgccgtgttc ggtgacgacg ccgtccgctg accggaggag catgaagacc
      781 aacgccctgc gcgccgccgg caccgcccta gccgtcgctc tcgcgagcgc ggtgcatgcc
      841 gacccgccgc tgctcgacct tgcgaccttc ccgcgcgccg cgctcgagat cgtgcagccc
      901 gtgccgcagc cggcgcgcag gtttcacttc gacgtgtggg tcgcggacac gccggcgcgc
      961 gccgagcagg gcctgatgtt cgtgcgcgat ctgccggccg ggcgcggcat ggtgtttccc
     1021 ttcgagcccg cgcgcgtcgc caccttctgg atgaggaaca cgtacatcga actcgacctg
     1081 ctgttcatcg ccgccgacgg gcgcgtggcg aagatcatcg agcgcgcggc accgctgcgg
     1141 gacgatcgct tgagctccga tgtacgggtt ggggcagtgc tcgagctgcg cggaggcgag
     1201 gtggggctac tgggactccg ggtgggagac caggtcagct ggaagccggc cggcaaggcg
     1261 tccgcacctt aagcgctcag cctccggcgg gcacggcgtc gaacgacacg gcggtgcgcg
     1321 gctgcgagcc gaggatcggg ctcgtgccgt gccacatgta cgagggaaac agcacgagct
     1381 gtccggcgtg cggctgcacg acgcaaccga cccccgcccc cggggtcgga aagcgcggtt
     1441 cgcccagcct gagccagccg ctcttgagct ccgcgtcacc cgcctcctcc ggcacttcga
     1501 cgtagtaggc cgagctgatc cagccgtggt tgtggaaatg gttgacgtga aagccgccgc
     1561 gcccgagccg caccgaccag gccgacgcga tctgcgcgtc gccctggttg cgcccgctga
     1621 agggatgatc gctcccgcgg ccgatgcccc cgaggtacgc tcgcagcggc tctgcga
//
LOCUS       DATLKL010000630         2720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_154569, whole genome shotgun sequence.
ACCESSION   DATLKL010000630 DATLKL010000000
VERSION     DATLKL010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154569"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..573)
                     /locus_tag="VM713_08510"
     CDS             complement(106..573)
                     /locus_tag="VM713_08510"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HVH84342.1"
                     /translation="MRTVAQWLGEYGASHENPTNKLLHWLCVPPIVLSVFGFLWSLPV
                     PAAFADASPWLNWATLAAAATVVYYFALSPALAAGVLLAFLVLLLVTHALAQLPWPLW
                     LSSLVIFIVAWIGQFVGHAIEGKRPSFFKDVQFLLIGPLWLLAAAYRRFGLPY"
     gene            complement(781..1656)
                     /gene="rpoH"
                     /locus_tag="VM713_08515"
     CDS             complement(781..1656)
                     /gene="rpoH"
                     /locus_tag="VM713_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HVH84343.1"
                     /translation="MTTGTALVARWGDQALAGPLGSLDAYLERVGRIPVLSREEERAL
                     AERFRSQSDLEAARQLVLSHLRFVVHIARGYSGYGLPTSDLIQEGNVGLMKAVKRFDP
                     TLNVRLVSFAVHWIRAEIHEYVLRNWRLVKIATTKAQRKLFFNLRRLKKNLSWLTAEE
                     TAAVARDLGVSPGEVTEMEKRLAARDLSFDPSPEGDDEESYSPAAYLPAPDADPAVSV
                     ENAESADDSATRLKSALSRLDARSRDIVEQRWMSDDNKATLHELADKYGVSAERIRQI
                     ESSALGKLRGLMAAA"
     gene            1882..2442
                     /locus_tag="VM713_08520"
     CDS             1882..2442
                     /locus_tag="VM713_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84344.1"
                     /translation="MISLTALWLPILVSAVIVFIASSVIHMAPLWHRNDFPKMPREEE
                     VLQALRPFAIPPGDYFLPRPGRREEMRSPEFQEKLTKGPIAVMTVLPNGMFNMTKNLV
                     QWFVFLLVVGVFVAYLTSRTLGAGTPYLKVFQIAGATAFIGYSLALCELSIWYRRSWG
                     LTLKAAFDGLIYASLTAGTFGWLWPR"
BASE COUNT          479 a          890 c          906 g          445 t
ORIGIN      
        1 cccacgcccc accagcccga aatgtgctgc gcgtgcgccg cggcacacgg ggagagcagc
       61 acggccgcca acagcatggt gcgggcgccg gccacggcac agccctcagt acggcagacc
      121 gaagcggcgg tacgccgccg caagcagcca cagcggcccg atgagaagaa actgcacgtc
      181 cttgaagaag gacgggcgct tgccctcgat tgcgtggccg acgaactgcc cgatccacgc
      241 cacgatgaag atcacgagcg agctcagcca cagcggccag gggagttgcg cgagcgcgtg
      301 cgtcaccagg agcagcacga ggaatgcgag cagcactccg gcggcgagcg ccggcgagag
      361 ggcgaagtag taaaccaccg ttgcggcggc ggcgagggtc gcccagttga gccagggcga
      421 ggcgtcggcg aaggccgcgg gcacgggaag cgaccagagg aacccaaaca ccgagagcac
      481 gatcggcgga acgcacagcc agtgcaggag cttgttggtc gggttctcgt ggctcgcgcc
      541 gtactcgccg agccattgcg ccacggtacg catgcgcggc gacgctacgc cccgcgcctg
      601 cggcgcgcaa gctggcttat tggccgaaat tgcctgcggc aatttcggcg gacatcactg
      661 cgattgccgc cggcaaaagg cggggctcga tgcgcatccg ccacgatcac ctcggcagcc
      721 gagcacttcg tggcgaggtt ggcgcagggg tttttgccgg ccgcgcttaa ggggacgctc
      781 tcaggcggcg gccatgaggc cgcggagctt gccgagcgcg ctcgactcga tctggcggat
      841 gcgctccgcc gacacgccgt acttatcagc gagctcgtgc agcgttgcct tgttgtcgtc
      901 cgacatccag cgctgctcga cgatgtcgcg gctgcgggca tcgaggcggc tcaacgcgct
      961 cttcaggcgc gtcgcgctgt catcggcgct ctcggcattc tcgaccgaga cggccggatc
     1021 ggcatccggc gccggcaggt aggcggcggg actatagctc tcctcgtcat cgccctcggg
     1081 tgaggggtcg aacgacaggt cgcgcgccgc caggcgcttt tccatctcgg tgacttcacc
     1141 gggactgaca ccgaggtcgc gcgccaccgc cgcggtctcc tccgccgtca gccagctgag
     1201 attcttcttc aggcgcctga gattgaagaa cagcttgcgc tgggccttgg tggtggcgat
     1261 cttcaccagg cgccagttgc gcagcacgta ctcgtggatc tcggcgcgaa tccagtgcac
     1321 cgcgaaggac accagccgca cattgagcgt cggatcgaag cgcttcacgg ccttcatgag
     1381 gccgacgttg ccttcctgga tcaggtcgga ggtagggagc ccgtagccgc tatagccgcg
     1441 agcgatgtgc accacgaagc gcaggtgcga cagcaccagc tggcgagccg cctcgagatc
     1501 gctctgcgat cggaagcgct cagccagcgc gcgctcctcc tcgcgcgaga ggaccggaat
     1561 gcggccgacg cgctcgaggt aggcatcgag gctaccgagc gggccggcga gtgcctggtc
     1621 gccccaacgg gcaaccagtg cggtacctgt ggtcatcaga tctcccgtgg attgggtggg
     1681 gattctagca gtcacgtagt tagagtgcta aaggaggcgg aagttccacc gaaggcccgt
     1741 caatagccct tgacgaacaa tagcttggac gcgccctccc accctgggga atcccccatc
     1801 cggggggctc gggggttcct catccggtca ccctccgata gagtctcgtc ggctcgacta
     1861 agagaccatg aaggggggct catgatcagt ctgaccgcgc tgtggctgcc gattctcgtc
     1921 tccgccgtga tcgtcttcat cgcgagctcg gtgattcaca tggcgccgct ctggcatcgg
     1981 aacgacttcc caaagatgcc cagggaagag gaagtactgc aggcgctgcg gccgttcgcg
     2041 attccccccg gcgactactt tctgccgcga cccggcagga gagaggagat gaggtccccg
     2101 gagttccagg agaagctgac caagggcccc atcgcggtga tgaccgtcct gccgaacggc
     2161 atgttcaata tgaccaagaa cctggtgcaa tggttcgtgt tcctcctcgt ggtcggggtc
     2221 ttcgtcgctt acctcacgag ccgcaccctc ggcgcgggca cgccctatct caaggtgttc
     2281 cagatcgccg gcgccaccgc cttcatcggc tactcgctcg cactgtgcga gctttcgatc
     2341 tggtaccggc gctcgtgggg tctcacgctc aaggccgcct tcgacggcct gatctacgcc
     2401 tcgctcaccg ccggaacctt cggctggctc tggccccgct agacgagtac cgcatcacac
     2461 gcgcggcagc ttatgtccgt gccgcggggc gcggtcgagc ggtgcgcgcc acgcgccgcc
     2521 gcagcggcac caaaagttaa gagtgcgtca cgccggcctg acataaggaa tgatttgata
     2581 gactcaaacg agctcgagcc tcagggaggc gtttgatcga tgaatcagcg ttgtggcaca
     2641 tggatcgtcg cactggcgtg cctgctcgcc gccgctacgg cggctgcgca gaaccagggt
     2701 caggtggtgc gcgtcaagct
//
LOCUS       DATLKL010000631         1529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_131870, whole genome shotgun sequence.
ACCESSION   DATLKL010000631 DATLKL010000000
VERSION     DATLKL010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131870"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VM713_08525"
     CDS             complement(<1..817)
                     /locus_tag="VM713_08525"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HVH84345.1"
                     /translation="MGAPSNQQVLEVLSRIQDPDLGRDVVSLGMIKELEVSPQGKVSF
                     TFELTTPACPVRDRFKTQAEDAVNEIPGVSGVEVRMTANVRPAFMRPKPSEILPGVKQ
                     TIAVASGKGGVGKSTVAVNLAAALRKSGASVGLLDADILGPSVPVMTGSLVHPEQSNG
                     RLQPIEAHGLKVMSFGNVRPPGEVTVFRGPMVGKMIEELLRLTDWGRLDYLVIDLPPG
                     TGDASLTLGQVIPLTGVAIVCTPQDVATNIAVNALQMFRKLNVTPLGLIENMSW"
     gene            868..>1529
                     /locus_tag="VM713_08530"
     CDS             868..>1529
                     /locus_tag="VM713_08530"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006415.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HVH84346.1"
                     /translation="MTAESSAADLVAYYGHAAADVYFQRAQKTLAGAKVDAVVAMDFF
                     ASQAGVLCGVAEAVRLLSEILEEGDEAYAIGEGEPMASKETVLRVRAPYSRFGVYETA
                     LLGMLSSCSGWATAAREIVEAANGKRVISFGARHVHPLIGPTMEYSAVVGGCAGCATP
                     LGAQLAGLQGASGTMPHAMILLFGDTVKAAEAFDRYMGDDVLRIVLVDTFKDEAEEAL
                     RVA"
BASE COUNT          241 a          486 c          547 g          255 t
ORIGIN      
        1 accagctcat gttctcgatg agaccgagcg gcgtgacgtt gagcttgcgg aacatctgca
       61 gcgcattgac cgcgatgttc gtcgcgacgt cctgtggggt gcaaacgatg gcgacgccgg
      121 taagagggat cacctggccc agcgtcagag atgcgtcgcc ggtcccgggc ggcaggtcga
      181 tcacgaggta gtcgaggcgg ccccaatcgg tcagtcggag cagctcctcg atcatcttgc
      241 cgaccatcgg tccgcgaaag accgtcacct ctcccggcgg tctcacgttt ccgaacgaca
      301 tcaccttcag gccatgagcc tcgatcggct gcaggcggcc gttcgactgc tcagggtgca
      361 cgagcgagcc ggtcataacc ggcaccgatg ggccgaggat gtcggcgtcg agcaacccga
      421 cgctcgcgcc gctcttacgc agagccgcgg cgaggttgac ggcgacggtg gacttgccga
      481 cgccgccctt gccggaggcg acggcgatcg tctgcttcac accggggagg atctcggacg
      541 gcttcgggcg catgaacgca ggccgcacgt tggcggtcat acgaacctcg acgccgctca
      601 cgccggggat ctcgttgacc gcgtcctcgg cctgcgtctt gaagcggtcc cgcacggggc
      661 aggccggcgt tgtcagctcg aaggtgaagc tcaccttgcc ttgcgggctg acctcgagct
      721 ccttgatcat gccgaggctg accacgtcgc ggccgaggtc ggggtcctgg atccggctca
      781 ggacctcgag cacctgctgg ttcgaagggg cgcccatatc ggcaccggat gttaccgagg
      841 ctgttaagtt catgcgctgt gaggcagatg acggccgaga gttcggcggc tgatctcgtc
      901 gcctactacg gccacgccgc ggccgacgtc tacttccagc gcgcgcagaa aacgcttgcc
      961 ggggcgaagg tcgacgcggt cgtcgcgatg gatttcttcg cgagccaggc gggcgtgctc
     1021 tgcggcgtgg cagaagcggt ccggctgctg agcgagatcc tcgaggaggg cgacgaggcc
     1081 tacgccatcg gcgagggcga gccgatggca tcgaaggaga ccgtgctgcg tgtccgcgcg
     1141 ccgtactcgc gtttcggcgt ctacgagacg gcgctgctcg ggatgctctc gagctgctcg
     1201 ggctgggcga cagcggcgcg ggagatagtc gaggccgcga acggcaagcg tgtcatcagc
     1261 ttcggcgccc ggcacgtcca tccgctgatc ggcccgacga tggagtactc ggcggtcgtc
     1321 ggcgggtgcg ccggctgcgc gaccccgctc ggcgcgcagc tcgcgggcct gcagggcgct
     1381 tcaggaacga tgccgcacgc gatgatcctg ctgttcggcg acaccgtgaa ggccgcggag
     1441 gctttcgacc ggtacatggg agacgacgtg ctgcggatcg tgctcgtcga caccttcaag
     1501 gacgaggccg aggaggcgct gcgtgtggc
//
LOCUS       DATLKL010000632         2617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_165607, whole genome shotgun sequence.
ACCESSION   DATLKL010000632 DATLKL010000000
VERSION     DATLKL010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165607"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VM713_08535"
     CDS             <1..1125
                     /locus_tag="VM713_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HVH84347.1"
                     /translation="IIALAAVTNYPLLALSGSIGTIVAILALLPPWVLLYLVSGKEGM
                     KGGWPLAIVGSLGYIAGQYPVARYWGPYLPDVAGAIVCFAALLVLLKVWQPKTVLGYG
                     GVPVDAAAAQQTQSHGLKTGEVLQAWLPFLVLLVVVACWTGPWSTLPKVSWFKATAVG
                     CSALATTCTAAKPSLTAVFNFAPYIAGTAILTTWIIVVLLLIAMGKLKGGQLGEVFRR
                     SFHQMWGALLVGVFIFGLAYTFNYSGMAASLAKGFASLGTAFIVVCPILGFIGVALSG
                     SNTSTNAMFGKFQALVGAQLGMPPLLLPSFNSVGAEIGKPVAPQTASVGVSTSKFVRK
                     EGDVIRHNMGWTFILLAYLIIIAIAFYLVRPDVMALGAGG"
     gene            complement(1134..2336)
                     /locus_tag="VM713_08540"
     CDS             complement(1134..2336)
                     /locus_tag="VM713_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008002122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVH84348.1"
                     /translation="MAKAAESLQPAARVPALDPLDLYGVRAALTEEERLVQDSVARLT
                     DEEVLPVIQRCFEEHRFPRELIPRLASQGLLGSSISGYDCAGLNAICYGLICQELERG
                     DSGIRSFVSVQSSLCMYPIFAYGSEEQKQRYLPRMARGEVIGCFGLTEPHGGSDPANM
                     RTQARRRGTDWVINGAKMWITNGAIADLGVIWAMTPDGIRGFLVEKGTAGFSAPEIHH
                     KFSLRASVTAALYFDNVVVPDSQLLPGVVGLKGPLSCLTQARYGIAWGVIGAAQACLR
                     QLLDYTASRELFGRPLAANQTIQVRLADMARRITTAQLLALQLGRMKDAGTMTPAHVS
                     LAKWNNCRMALDVARDCRDMLGGAGISAEFAPIRHMLNLESVITYEGTETIHQLTIGR
                     ELTGISAF"
BASE COUNT          387 a          867 c          915 g          448 t
ORIGIN      
        1 atcatcgcgc tcgccgccgt taccaactat ccgctgctgg cattgtcggg ctcgatcggc
       61 accatcgtcg cgatactcgc gctgctgccg ccgtgggtgc tgctgtacct ggtctccggc
      121 aaggaaggca tgaagggcgg ctggccgctc gcgatcgtcg gctcgctcgg ctacatcgcc
      181 gggcagtatc cggtggcgcg ctactgggga ccgtacctgc cggatgtcgc cggcgccatc
      241 gtctgcttcg cggcgctgct ggtgctcctc aaggtgtggc agccgaagac ggtgctcggc
      301 tacggcgggg tgccggtcga tgccgccgcc gcgcagcaga cgcagtcgca tggcctgaag
      361 accggcgagg tgctgcaggc ctggctgccc ttcctggtgc tgctggtcgt ggtcgcgtgc
      421 tggaccgggc cgtggtcgac gctgcccaag gtcagctggt tcaaggcaac ggcggtcggc
      481 tgctcggcgc tcgccaccac ctgcacggcg gccaagccga gcctcacggc ggtgttcaac
      541 ttcgccccgt acattgccgg caccgcgatc ctcacgacct ggatcatcgt agtgctactc
      601 ctgatcgcaa tgggcaagct caagggcggc cagctcggcg aggtgttccg gcgcagcttc
      661 caccagatgt ggggcgcgct gctggtcggc gtgttcatct tcgggctcgc ctacaccttc
      721 aactactcgg ggatggccgc ctcgctcgcc aaggggttcg cgagcctcgg cacggccttc
      781 atcgtcgtct gcccgatcct cggcttcatc ggtgtggcgc tctcgggatc gaacacctcg
      841 accaacgcca tgttcggcaa gttccaggcg ctggtggggg cgcagctcgg catgccgccg
      901 ctgctgctgc cgtcgttcaa ctcggtgggc gccgagatcg gcaagcccgt cgcgccgcag
      961 accgcgagcg tcggcgtctc gaccagcaag ttcgtgcgca aggaaggcga cgttatccgc
     1021 cacaacatgg gctggacgtt catcctgctc gcgtacctga tcatcatcgc gatcgcgttc
     1081 tatctcgtgc ggccggacgt gatggccctc ggcgcgggcg gctagtaact tcgctaaaac
     1141 gcgctgatgc cggtcagctc gcgtccgatc gtgagctggt ggatggtctc agtgccctcg
     1201 taggtgatga cgctctcgag gttgagcatg tggcggatgg gggcgaactc ggcgctgatg
     1261 ccggcgcccc ccaacatgtc gcggcagtcg cgcgccacgt cgagcgccat gcggcagttg
     1321 ttccacttgg ctagcgagac gtgcgccggc gtcatggtac ccgcgtcctt catccgcccg
     1381 agctgcagcg ccaggagctg cgcggtggtg atgcggcgcg ccatgtccgc gaggcgcacc
     1441 tggatcgtct gattggcggc aagcggccgg ccgaagagct cgcggctggc ggtgtagtcg
     1501 agcagctgcc gcaggcacgc ctgcgccgcg ccgatcactc cccaggcgat gccgtagcgc
     1561 gcctgcgtga ggcaggagag cgggcccttg agtcccacca ctccggggag cagctgcgag
     1621 tccggcacca cgacgttgtc gaagtagagc gccgcggtca ccgaggcgcg cagcgagaac
     1681 ttgtggtgga tctcgggcgc ggaaaacccc gccgtgccct tctcgacgag gaagccgcgg
     1741 atgccgtcgg gcgtcatcgc ccagatcacg ccgaggtcgg cgatggcgcc gttggtgatc
     1801 cacatcttgg cgccgttgat cacccagtcg gtgccgcgcc gcctggcctg cgtcctcatg
     1861 ttggcgggat ccgagccccc gtgcggctcg gtgagcccga agcagccgat cacctccccg
     1921 cgcgccatgc gcggcaggta gcgctgcttc tgttcctcgg agccgtaggc gaagatcgga
     1981 tacatgcaca gcgaggactg caccgacacg aagctgcgga tgcccgaatc cccgcgctcg
     2041 agctcctggc agatgagtcc gtagcagatg gcgttgagcc cggcgcagtc gtagccgctg
     2101 atggaggagc cgagcagccc ttggctcgcg agccgcggga tcagctcgcg cggaaagcga
     2161 tgctcctcga agcagcgctg gatgaccggc agcacttcct catccgtgag ccgggcgacc
     2221 gagtcctgca ccagccgctc ctcctcggtg agcgcggcac gcaccccgta gagatcgagc
     2281 gggtcgaggg cggggactct cgctgcgggc tgcagggatt cggcggcttt ggccacggag
     2341 ggcactcctc gatgcgggcg cgggcgcgta tcgtatccta agaggagcgg cggggattta
     2401 acgagcgcag ctttgacgct cgccgcgggg gcgtccacat gaagattccc gagattctca
     2461 tggtcgagac cgtgcaccgg cccggaagcc tcgccaaggt gctgcaggtg atcgcggatg
     2521 ccgggctcac gatcgagcac ctgacggcgc tgcggcgcga gcagggccgg acgctctggg
     2581 agatcaccgt cgatttggac gaggagacgg accgcag
//
LOCUS       DATLKL010000633         2802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_98114, whole genome shotgun sequence.
ACCESSION   DATLKL010000633 DATLKL010000000
VERSION     DATLKL010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98114"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VM713_08545"
     CDS             complement(<1..713)
                     /locus_tag="VM713_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH84349.1"
                     /translation="MMRALVALCVRHHGSVTALSVLALVLGAWGALHSPLDVFPEFVP
                     SQVDVQTEAPGFAPQQVEELVTKPVENAINGAAGLATLRSESIPGLSVVTVTFASGVD
                     VHVARQGIAERLAELGSTLPAGVATPKLSPLVSSTMDLLKIGLLSARVDAYTLRDAAD
                     WVIKPRLLAVPGVARVIVYGGDARQIQILPDVDRLASYNVTIAEIADAARTALPLRGA
                     GFIDLPAQRVLIKSPTPAPT"
     gene            complement(720..1697)
                     /locus_tag="VM713_08550"
     CDS             complement(720..1697)
                     /locus_tag="VM713_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84350.1"
                     /translation="MNRIQMPFMSMALALSMVWVAGCQRAPSAETAQPAAAGKEAASV
                     EAEGLTLKPDEIEKAGIKTTPASAARRAPETAGYALVIAREAIAQAVADLSSATAVAR
                     QSQAALARSRGLAGTPGAMPVESQEAAERQAAVDQAALTLAKRRVSAVYGSNAPWKDD
                     YASPLLASLASGEAKLTRVTFPLGALGAQMPSRLRLAHMGGAPGTKSFEVDTLWRAPA
                     DASLPGRSFFGILRSSDVADGERLLAFAPVGPAEDGVVIPYAAVVLSAGKYWCYLEEK
                     PGQFVRTEVDPGAPTDDGYFVKEGVAAGAQIVTASAGELLARELGTAAE"
     gene            complement(1694..>2802)
                     /locus_tag="VM713_08555"
     CDS             complement(1694..>2802)
                     /locus_tag="VM713_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVH84351.1"
                     /translation="HSSQGSGHELDWFAMLSASYEVDFWGKNRALARSALLQATASRA
                     DRDTVALTLLGGVADEYFQVLALRERIAIARENSAAADKVLAAVQARFDAGAATAVDL
                     ASQRAAYDTTRITLADLEQTEVEARAALALLLGRFPEQFEVAGTSLDPLREPAVAPGL
                     PAELLTRRPDIAMAEANLQAASADLKAERAALLPSLSLTVSGGAQIPALPATVLTIGG
                     TGPSLALVANLTQPIFDRGRLRAKRDETQARRQELLAEYRAVILAALVDAEKALSALD
                     HLKAVEEFEKGNVRENERAFAGARLRYEHGSGDFLTLLEAQRTLYAARDQFAQYKLAR
                     LQGLVALCKALGGGWSATSAAQAPAARRADGKSS"
BASE COUNT          416 a          998 c          969 g          419 t
ORIGIN      
        1 gtcggggccg gagtaggcga cttgatcagc acgcgctggg ctgggaggtc gatgaagccg
       61 gcgccgcgca agggaagcgc cgtgcgcgcg gcgtcggcta tctcggcgat ggtgacgttg
      121 tagctggcga ggcggtccac gtcaggcagg atctggatct gccgggcgtc accgccgtag
      181 acgatgacgc gggccactcc cggcaccgcg aggagccgcg gcttgatcac ccagtcggcg
      241 gcatcgcgca gcgtgtaggc gtcgaccctg gcggacagga ggccgatctt caggaggtcc
      301 atggtgctcg agacgagcgg cgacagcttc ggcgtcgcca cgccggccgg cagcgtgctg
      361 ccgagctcgg ccagacgctc cgcgattccc tggcgcgcaa cgtgtacatc caccccgctc
      421 gcgaacgtca cggtcacgac cgagagcccc gggatggact cggaacgcag cgtcgcgagc
      481 cccgcggcgc cgttgatggc gttctcgacg ggcttggtga ccagctcctc gacctgctgc
      541 ggcgcgaatc ccggcgcctc ggtctggacg tcgacctgcg agggaacgaa ctccggaaag
      601 acatcgagcg gcgagtgcag cgcgccccac gcacccagca ccagggcgag caccgacaga
      661 gcggttaccg agccatggtg ccgcacgcac agtgcgacca acgcgcgcat catctgcgtc
      721 tactccgccg ccgtgcccag ctcgcgcgcc agcagctcgc cggccgaggc ggtgacgatc
      781 tgcgcgcccg cggcaactcc ttccttgacg aagtagccgt catcagtcgg cgcgccggga
      841 tcgacctccg tacgcacgaa ctgccccggt ttctcctcga ggtagcacca gtacttgccg
      901 gcgctgagca cgaccgctgc atacgggatg acgaccccat cctccgccgg gccgaccggt
      961 gcgaaggcga gcaggcgttc cccgtcggcg acgtcgctgc tgcggagaat gccgaagaag
     1021 ctccggccgg ggaggctcgc gtcggccggc gcgcgccaca aggtgtcgac ttcgaagctc
     1081 ttggtgccag gcgctccgcc catatgcgcc aggcgcagtc gcgatggcat ctgtgccccg
     1141 agcgcgccga gcggaaaggt gacgcgggtc agcttcgcct cgccgctcgc cagtgatgcg
     1201 aggagcggac ttgcgtagtc gtctttccag ggcgcgttgc tgccatacac ggcggataca
     1261 cgccgcttgg ccagcgtgag cgccgcctga tcgaccgccg cctggcgctc cgcggcctcc
     1321 tgggactcga ccggcatggc gcccggcgtg ccggcaagcc cgcggctgcg ggcgagcgct
     1381 gcctggctct gccgcgcaac cgcggtcgcc gagctcaggt cagcgacggc ctgggcgatg
     1441 gcctcgcgcg cgatcacgag cgcgtagcct gcggtttccg gggcgcggcg cgccgccgag
     1501 gccggagtcg tcttgatgcc ggccttttcg atctcgtcgg gcttgagtgt aaggccctcc
     1561 gcttcgacgc tcgcagcctc cttgcccgct gcggcgggct gcgctgtttc cgccgacggc
     1621 gcgcgctgac agcccgcgac ccacaccatg ctgagcgcga gggccatgga catgaatggc
     1681 atttggatcc ggttcatgag gatttcccat ccgcccgccg cgccgccggc gcttgcgccg
     1741 cactcgtggc gctccagccg ccgcccaggg ccttacacag cgcgaccagg ccctgcagcc
     1801 gcgccagctt gtactgcgcg aattgatcgc gtgcggcgta cagcgtgcgc tgggcttcga
     1861 gcagcgtgag gaagtcaccg gagccgtgct cgtagcgcag ccgcgccccg gcaaaggctc
     1921 gttcattctc gcgcacgttc cccttctcga attcctcgac ggccttgagg tgatcgagcg
     1981 ccgagagcgc cttctcggca tcgaccaggg ccgcgagaat gaccgcccga tactcggcga
     2041 gcagctcctg ccgtcgcgcc tgtgtctcat cccgcttggc gcgcagccgc ccgcgatcga
     2101 agatcggctg ggtgagattc gcaaccaggg ccagggatgg tccggtgcca ccgatagtca
     2161 gaaccgtcgc cggcagcgcc gggatctgcg cgccgccgct cacagtgagt gaaagactcg
     2221 gtaacagcgc ggcccgctcc gccttcaggt ccgcgctcgc cgcctgcaga tttgcctcag
     2281 ccatcgcgat gtcgggccgc cgcgtgagca gctccgccgg gaggccgggt gcgactgcgg
     2341 gttcgcgcag cgggtcgagc gaggtgccgg cgacctcgaa ctgctccgga aagcggccga
     2401 gcaacaaggc cagtgcagcg cgtgcctcga cttcggtctg ctcgaggtcc gcgagggtga
     2461 tccgcgtcgt gtcgtaggcc gctctctgcg aagcgagatc taccgcggtg gcggccccgg
     2521 catcgaagcg cgcctggacc gccgcgagca ccttgtcggc cgcggccgaa ttctcgcgcg
     2581 caatcgcgat ccgctcccgc agcgcgagca cctgaaaata ctcatccgcg actccaccca
     2641 gcagcgtcag ggcgacggtg tcccggtccg cccgcgaggc ggtggcctgg agcagtgccg
     2701 accgggcgag cgcgcgattc ttgccccaga agtcgacctc gtagctggct gacagcatcg
     2761 cgaaccaatc gagctcgtgt cccgagccct gcgaggaatg cc
//
LOCUS       DATLKL010000634         3452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_221694, whole genome shotgun sequence.
ACCESSION   DATLKL010000634 DATLKL010000000
VERSION     DATLKL010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221694"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2508
                     /locus_tag="VM713_08560"
     CDS             <1..2508
                     /locus_tag="VM713_08560"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84352.1"
                     /translation="VQDDLRILRDLVPGADLYMRQSASPSAIETNITARVTAGARYDV
                     KDVDVSADGTRVVFAMRGPLTKGQQAKTPPCWRIWQYVIATDTLAPVINPTIDPDPLT
                     VNDVSPHYLADGRIVFSTTRQDTSQGVLLDEHKPGFIALDEASAADQGGTQPDFVLEV
                     MNADGTHVHQITFNPSHDQQATVLQNGRLLYSRWDDAAGHDAMSLYSANPDGTDVELY
                     YGANSHLTGAPAPGTTTPTVVEFTQPHEREGGSVMALLRQYTGVDDGGNLVLIDGVHY
                     VENTQTLQGPPYNFSRASYPGPAQTQATPLNNIYTFPGPSPGGRFNSGYPLWDGTGRI
                     LVSWSECRLLDTTQTPPAIVPCTTAGLANPNAQSAPPLYSLWMLDPTQGTMIPIVAPV
                     EGVMITYVVATQPKPVPNIILDTVPTGQAGAFASAGVGVIDIRSVYDIDGFAVNPASG
                     AALNIGTIADPAQTPAAQRPARFMRFEKVVSIPDKTVQALTNADFGVSSYMMEILGYA
                     PIEPDGSVRVEVPANVAFRVSVLDANARAITAPQGVWLQLIPGETLSCNGCHKPQGGA
                     QNPLSHGRQGAFASAWVGGTAGTAFPHTIATGAAAFIPQSNGETMAEARMAVSCASDT
                     PPCLQMMPSVNVLYTDVWTDPAQAMPGAPITYSYTDATNPVPAIPTTALCATSWSSAC
                     RIIINYPQHIQPIWDAARTGMLNGVPGSVTCSQAGCHNPTNAAGAAQTPAGNLDLTHS
                     VDANTNEFTSYGQLLTPHTTVIMGLPGPTVGPFMNAGSANGGASSAFFNCFATGSGCN
                     NPSHTGWLNIAELRLLSEWLDIGAQYHNDPFAPAAPM"
     gene            2534..3286
                     /locus_tag="VM713_08565"
     CDS             2534..3286
                     /locus_tag="VM713_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HVH84353.1"
                     /translation="MQRPARAALLACAALLAVLAPAVSAAREYLQLFVAEPYLELHTG
                     PGRGYPVFHVVPRDESVDVLFRRTDWFKVRTERGVEGWASQADMLKTVLADGSPFKFE
                     LGDLAGFSSHRYELGLFAGEYGGANLISAYWSVSLNPQMAVEAAAGQFLGRFSNGETG
                     DLGFAHVISPDTRWSPLLLLGVGIVHTEPKATLVTPSNRTEQTAYVGGGIRYYLTRRF
                     FMRAEYKAHYIFTKRNQNEEADEWKLGFAFFF"
     gene            3256..>3452
                     /locus_tag="VM713_08570"
     CDS             3256..>3452
                     /locus_tag="VM713_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84354.1"
                     /translation="METGIRVLLLTLALALLPGCATVRNWFHREHAAAPSSETQAGAP
                     SGASEPVATGPQEETPLRVIEP"
BASE COUNT          588 a         1236 c         1099 g          529 t
ORIGIN      
        1 gtgcaggatg acctgcgtat cctgcgcgac ctggtgccgg gcgccgatct ctacatgcgc
       61 caaagcgcct caccgagcgc catcgagacc aacatcaccg cgcgcgtcac ggccggggcc
      121 cgctacgacg tgaaggacgt cgacgtctcg gccgacggca cccgggtggt attcgcgatg
      181 cgcggcccgc tcaccaaggg tcagcaggcc aagaccccgc cctgctggcg catctggcaa
      241 tacgtgatcg ccaccgacac actggcgccg gtcatcaatc ccaccatcga tcccgacccg
      301 ctgaccgtca acgacgtgtc gccgcactat ctggcggacg ggcgcatcgt cttctcgacc
      361 acgcgccagg acacctcgca gggcgtgctg ctcgatgagc acaagcccgg gttcatcgcc
      421 ctcgacgagg cctcggcggc cgaccaggga ggcacccagc cggatttcgt gctagaggtg
      481 atgaacgccg acggcacgca tgttcaccag atcaccttca atccgagcca cgatcagcag
      541 gcgacggtcc tgcagaatgg gcggctgctc tacagccgct gggacgatgc cgcgggacac
      601 gacgccatgt cgctctactc cgccaatccg gacggcaccg acgtcgagct ctactacggc
      661 gccaacagcc acctgaccgg cgcgccggcg ccgggcacga ccacgccgac ggtggtcgag
      721 ttcacgcagc cacacgagcg cgagggcggc agtgtcatgg cgctgctccg ccagtacacc
      781 ggcgtcgacg acggcggcaa tctcgtgctc atcgatggcg ttcactacgt ggagaatacc
      841 cagactctgc agggaccccc gtataacttc agccgcgcca gttaccccgg cccggcacag
      901 acgcaggcca ccccgctcaa caacatctat acgtttcccg ggccctcgcc ggggggacgc
      961 ttcaactcgg gctatccgct gtgggacggt actgggcgca ttctcgtcag ctggtcggaa
     1021 tgccggctgc tcgataccac gcagacgccg ccggcgatag tgccgtgcac caccgccggc
     1081 ctcgccaatc ccaacgcgca gagcgccccg ccgctctaca gcctgtggat gctcgacccg
     1141 acgcagggca cgatgatccc gatcgtggcg ccggtcgagg gcgtcatgat cacctacgtc
     1201 gtggcgacgc agcccaagcc cgtccccaac atcatcctcg acacggtgcc gaccgggcag
     1261 gccggggctt tcgccagcgc cggcgtcggc gtcatcgaca tcaggagcgt ctacgacatc
     1321 gacggcttcg cggtgaatcc ggcgagcggc gcggcgctga acatcggcac catcgccgat
     1381 cccgcccaga cgccggcggc ccagcgtccg gcgcgcttca tgcgtttcga gaaggtagtc
     1441 tcgatccctg acaagaccgt gcaggcgctg accaacgccg acttcggcgt cagcagttac
     1501 atgatggaga tcctcggcta cgctcctatc gagccggacg gctcggtgcg cgtcgaggtg
     1561 ccggccaacg tcgcattccg ggtgagcgtg ctcgatgcca atgcacgcgc catcacggcg
     1621 ccgcaggggg tgtggctgca gctcatcccg ggcgagacgc tctcctgcaa cggctgccac
     1681 aagccccagg gcggcgccca gaacccgctg tcccacggcc gccagggcgc cttcgcgagc
     1741 gcctgggtcg gcggcacggc cggcaccgcc ttcccgcata ccatcgcgac cggtgcggcg
     1801 gcgttcattc cgcagagcaa cggcgagacc atggccgagg cgcgcatggc ggtgagctgt
     1861 gcgagcgaca cgccgccgtg cctgcagatg atgccgagcg tcaacgtgct ctacaccgac
     1921 gtctggaccg accccgcgca ggcgatgccc ggcgcgccca tcacctacag ctatacggac
     1981 gcaaccaacc cggtcccggc gattccgact accgcgctgt gtgcgacttc ctggtcgtcc
     2041 gcctgccgca tcatcatcaa ttacccgcag cacatccagc cgatctggga tgcggcgcgc
     2101 accggcatgc tgaacggggt gccagggagc gtcacctgct cgcaggccgg ctgccacaac
     2161 ccgaccaacg ccgccggcgc cgcgcaaacg ccggcgggga atctcgacct gacccacagt
     2221 gtcgacgcga acaccaacga gttcacctcc tacggacagc tgcttacccc tcacactacg
     2281 gttatcatgg gcctgccggg accgacggtc ggtccgttca tgaatgcggg tagcgccaat
     2341 ggcggggcgt caagtgcctt cttcaactgc ttcgcaacag gttccgggtg caacaacccg
     2401 tcgcatacgg gttggctgaa catcgcggag cttcgcctgc tgtcggagtg gctggatatt
     2461 ggcgcgcagt accacaacga cccgttcgcc cccgcggcac ccatgtgagc aagggaaaaa
     2521 cctgacgcgc cgtgtgcaac ggccggcgcg cgccgccctg ctggcgtgtg ccgcgctgct
     2581 cgccgtgctg gcgccggcgg tgagcgccgc gcgcgagtac ctgcagctgt tcgtggccga
     2641 gccgtatctg gagctgcaca ccggaccggg ccgcggctac ccggtgttcc acgtggtgcc
     2701 gcgtgatgaa agcgtcgatg tgctgttccg gcgcaccgac tggttcaagg tgcgcaccga
     2761 gcgcggcgtc gagggttggg ccagccaggc agatatgctt aagacggtgc tggcggacgg
     2821 ttcgccgttc aagttcgagc tcggcgatct ggccgggttc agctcgcacc gctacgagct
     2881 cggcctgttc gccggcgagt acggcggcgc gaatctgatc tccgcctact ggtcggtgtc
     2941 gctcaacccg cagatggcgg tggaagccgc cgccggtcag ttccttggcc gcttctccaa
     3001 cggcgagacg ggtgacttag gcttcgccca cgtgatttcc cccgacacgc gctggtcacc
     3061 gctgctgctg ctcggcgtgg gtatcgtgca caccgagccg aaggcgacgc tggtgacgcc
     3121 gtcgaaccgc accgagcaga ccgcctacgt gggcggcggc atccgctatt acctgacgcg
     3181 gcgttttttc atgcgcgccg aatacaaggc tcattacatc ttcacgaagc gcaaccagaa
     3241 cgaggaagca gacgaatgga aactgggatt cgcgttcttc ttctgacgct ggcgctcgcc
     3301 ctgcttccgg gctgcgccac ggtgcgcaac tggttccacc gcgagcacgc cgccgccccg
     3361 agcagcgaga cccaggccgg cgcgccgtcg ggggcctcag aacccgtcgc cacgggcccg
     3421 caggaagaga cgccgctgcg ggtgatcgag cc
//
LOCUS       DATLKL010000635         4337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_165647, whole genome shotgun sequence.
ACCESSION   DATLKL010000635 DATLKL010000000
VERSION     DATLKL010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165647"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1321
                     /locus_tag="VM713_08575"
     CDS             152..1321
                     /locus_tag="VM713_08575"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HVH84355.1"
                     /translation="MSLSPYGLFELPWWGYALTTFLTIQTMFLGVTLYLHRDQSHGGL
                     ILHPALRHLFRFWLWFSSGAVTREWVAVHRKHHAYADQEGDPHSPVVFGLKRVLFEGF
                     ELYRAAGRDPETIRNYGRGTPDDWLERHVYGRYNYAGIVMFVAAHLALFGVPGIIMIA
                     VHLSAQPFFAGGVINGLGHAVGYRSFEMPSTATNLLPWGLLFGGEELHNNHHAFPRSA
                     RFAAQRWELDIGWLWIRLFSAAGLARVRWVAPRPHLERRRHELDTDTVQALFTNRLHV
                     LRAYARRVVLPVCRELKRREPHGTVPAGTPKLLIRHPALLAEEARRRLRELLERYEVL
                     RRVVEYREGLQQAWEDASASQALTELREWCRRAEASGIAALREFARGLPAYASAR"
     gene            complement(1352..1828)
                     /locus_tag="VM713_08580"
     CDS             complement(1352..1828)
                     /locus_tag="VM713_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVH84356.1"
                     /translation="MELDRYDRALLRLLQQDARMTNTALAARVNLSESACLRRVRALE
                     ESGLIEGYTALVDQQRAGFPVNVFVNITLDRQSQTGLEAFEGAVRRIPEVMECYLMTG
                     EHDYLLRLVVTDLADFERIHNQHLTRLPSVARVHSSFAMRTVTRSRTLPMRQGGAG"
     gene            1968..3071
                     /gene="ald"
                     /locus_tag="VM713_08585"
     CDS             1968..3071
                     /gene="ald"
                     /locus_tag="VM713_08585"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HVH84357.1"
                     /translation="MRIGVPREIKVHEYRVGLVPAGVRELTAAGHEVLIETGAGNGIG
                     VDDAQFRGAGASIAANAAEVFQRSEMVIKVKEPQPAECLMLRPGQVLFTYLHLAADPE
                     QARGLMKSGATAIAYETVTGPKGSLPLLTPMSEVAGRMSIQVGAPSLQKAQGGFGILL
                     GGVPGVPPARVVILGGGVSGTHAAEMAVGLQARVTVVDRSVDRLRELSVQFGSTIQTA
                     YSTTETIEKLVRDADLVVGAVLVAGAAAPKLVTRAMIKTMKPGAVLVDIAIDQGGCFE
                     TSRPTTHAEPTFVVDGVIHYCVANMPGAVPRTSTLALTNATLPYARALADLGWREAFK
                     RDPGLAAGLNVHAGEITHEVVARALGLKARPLN"
     gene            complement(3079..3393)
                     /locus_tag="VM713_08590"
     CDS             complement(3079..3393)
                     /locus_tag="VM713_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84358.1"
                     /translation="MSRATTEQAAVKRWAQMVGHVGPVDWIVKKIGSHDVHAVCNSPQ
                     KDNLLDALTEGLGVIKCSGLMSEFMFNPTTLQFQNYLWGGYSTMQTADDTALEIGECS
                     KL"
     gene            3693..4268
                     /locus_tag="VM713_08595"
     CDS             3693..4268
                     /locus_tag="VM713_08595"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HVH84359.1"
                     /translation="MKTVLPLAVASLAAGAALAAPVTYQVDPAHTYPSFEADHFGGMS
                     VWRGKFDSSSGTIVLDREAGTGTVDISVVAGSVDFGHAKLNEHVKSPEMLDVQKYPTA
                     VYKGTLTNFKNGAPTEVHGELTLHGVTKPMNLKINSFVCKLTPLSKKERCGADASGSF
                     DRADFGVNYGDKYGFNMQVKLAIQVEAAPEG"
BASE COUNT          733 a         1453 c         1444 g          707 t
ORIGIN      
        1 aagcgcggcc gtggcgcacc gcgcggacca aagagcgccg cgtcccaaac tgtgaacggt
       61 gccccggcgg aagcagaaaa aatcgcgtcg ggctcacgga attgccgtac cctagccgcg
      121 cagactcacg caagaactcg acggagaatc catgagtctc tcgccgtacg gtctctttga
      181 gctgccttgg tggggctacg cgctcaccac cttcctcacc atccagacga tgtttcttgg
      241 tgtgacgctc tacctgcacc gcgaccagtc ccacggcggg ctgatcctgc atccggcgct
      301 ccgccatctg ttccggttct ggctgtggtt cagctcgggc gcggtgacgc gcgagtgggt
      361 ggcggtacac cgcaaacacc acgcctacgc cgaccaggaa ggcgatcctc acagcccggt
      421 cgtgtttggc ctgaagcgcg tgctgttcga gggtttcgag ctgtaccgcg cggccggccg
      481 tgacccggag accatcagga attatggccg cggtacgccg gacgactggc tcgagcggca
      541 cgtgtacggc cgttacaact acgccggcat cgtgatgttc gtcgccgccc acctggcgct
      601 gttcggcgtg ccaggcatca tcatgatcgc cgtgcacctc tcggcccagc cgttcttcgc
      661 cggcggcgtg atcaacgggc tcggccacgc cgtcggctac cgcagcttcg agatgccttc
      721 gacggcgacc aatctcctcc cctggggact gctgttcggc ggcgaggagc tgcacaacaa
      781 ccatcatgcg ttcccacgct cggcgcgctt cgccgcgcag cgctgggagc tcgacatcgg
      841 ctggctgtgg atacggctgt tcagtgcggc cggactggcg cgcgtgcgct gggtcgcccc
      901 gcgcccgcat ctcgagcggc gccgtcacga gctcgacacc gataccgtgc aggcgctctt
      961 taccaaccgc ctgcacgtgc tgcgcgccta tgcgcgccgc gtcgtgctgc cggtatgccg
     1021 cgagctcaag cggcgcgagc cccacggcac cgtgcccgcg ggcacgccaa aactgctgat
     1081 ccgccacccg gcgctgctgg ccgaggaggc gcggcggcgc ctgcgggagc tcctcgagcg
     1141 ctacgaggtg ctgcggcgcg tggtcgagta ccgcgaggga ctgcagcagg catgggagga
     1201 cgcatcggcg agccaggcac tcaccgagct gcgcgagtgg tgccgccggg cggaagcgag
     1261 cggcatcgcg gcgctgcgcg agtttgcccg gggattgccc gcctacgcgt cggcgagata
     1321 gtcatcagcg gcggcgcgca catccatgtg cctagccggc accgccctgt cgcatcggca
     1381 gcgtgcgcga gcgcgtcacg gtgcgcatcg cgaaactcga gtgtacgcgt gcgacgctcg
     1441 gcagccgggt gagatgctga ttgtggatac gctcgaagtc ggcgaggtcg gtgaccacga
     1501 gccgcagcag atagtcgtgc tcaccggtca tcaggtagca ctccatgacc tccggtatgc
     1561 gccgtaccgc gccctcgaag gcctcgaggc ccgtctggct ctggcggtcg agcgtgatgt
     1621 tgacgaatac gttcaccgga aatccggccc gctgctggtc gaccagcgcg gtatagccct
     1681 cgatcaggcc ggactcctcc agcgcccgca cgcgccgtag acaagcggat tccgagagat
     1741 tcacccgcgc ggcgagcgca gtgttggtca tgcgcgcgtc ctgctgcagc aggcgcagca
     1801 acgcgcggtc gtagcggtcg agctccatag cgcaagaatc cttcgaagac agcgcttccg
     1861 gcggcagatt ctgccacaca gccgggtgac cgggctgaaa atacgcaaac ctgtgcaccg
     1921 ggtgccgcgc tctaattcgc ccaccacgaa cgaaggggga gagcgccatg cgcatcggtg
     1981 ttccacgcga gatcaaggtg cacgagtatc gggtcggtct ggtgccggcc ggggtgcgcg
     2041 agctcacggc cgccggccac gaggtgctca tcgagaccgg cgccggcaac ggcatcggag
     2101 tcgacgatgc gcagttccgc ggcgccggcg cgagcatcgc ggcgaacgcc gccgaggtgt
     2161 tccagcgctc tgagatggtg atcaaggtca aggagccgca gccggccgaa tgcctgatgc
     2221 tgcgccccgg ccaggtgcta ttcacctacc tgcatctcgc cgccgatccg gagcaggcgc
     2281 ggggcctgat gaaatccggc gctacggcga tcgcctacga gaccgtgacg ggaccgaagg
     2341 gctcgctgcc gctgctcacc ccgatgagcg aagtcgccgg acgcatgtcg atccaggtcg
     2401 gagcacccag tctgcagaaa gcgcagggcg gcttcggcat tctgctcggc ggcgtgccgg
     2461 gcgtgccgcc ggcgcgcgtg gtgattctcg gcggcggggt ctccggaacg cacgcggcgg
     2521 agatggccgt ggggttgcag gcccgcgtga ccgtggtcga ccgctcggtc gaccggttgc
     2581 gggagctctc cgtccagttc ggctcgacaa ttcagaccgc ctactcgacc accgagacga
     2641 tcgagaagct ggtgcgggat gcggatctgg tggtcggcgc ggtgctggtg gcgggcgcgg
     2701 cggcgccgaa gctcgttacg cgcgccatga tcaagaccat gaagccgggg gcggtgctgg
     2761 tcgacatcgc catcgaccag ggaggctgct tcgagaccag ccggccgacc acccacgccg
     2821 aacccacctt cgtggtggac ggagtgattc actactgcgt cgccaacatg ccgggcgcgg
     2881 tgccgcgcac ctcgacgctc gcgctcacca acgccactct cccctatgcg cgcgctctgg
     2941 ccgatctcgg ctggcgggag gcgttcaagc gtgaccccgg gctcgccgcc ggactcaatg
     3001 tgcacgccgg cgagatcacc cacgaggtcg tcgcccgggc gctcggactc aaggcgcggc
     3061 cgttgaactg acagccggct agagcttgga acactcgcca atctcgagag cagtgtcgtc
     3121 cgcggtttgc atggtcgagt aaccgcccca caggtagttt tgaaactgca gggtggtggg
     3181 gttgaacatg aattcactca tgaggccgga acacttaata acccccaacc cctccgttaa
     3241 cgcatccagc aggttgtcct tctgcggcga gttacacacc gcgtgaacgt cgtggctgcc
     3301 aatcttcttc acgatccaat caacgggtcc gacatgacca accatttgcg cccagcgctt
     3361 caccgcggcc tgctcggtcg tcgcgcgcga cacgatgtac taggattcgg aggggaagtt
     3421 cacgggaacc catctctcgt cggtctcgct gtagtgaaac ccggtgacct gagaggcaac
     3481 gcacgaatag ccttcgccat gagcagcggc ggttaccgcc atcgccgcaa ccgagagtgc
     3541 ggctcgtcct cgatgcttca ttgcgcttta cctcgctgac ccaaggcgcg ggagcatgcc
     3601 atgagtgccc ctccctacaa aggagggtgg ctcgccgtcg gggcgagggc gtaggctagc
     3661 gccccccttg gctgaaccgt cggaggttcg tgatgaagac tgtgctgccg ctcgccgtgg
     3721 cgagcctcgc cgctggcgct gccctcgccg cgccggtcac ctaccaggtc gatccggctc
     3781 atacctaccc gagcttcgag gccgaccatt tcggcggcat gtcggtctgg cggggtaagt
     3841 tcgacagctc ctcgggaacg atcgtgctcg accgggaggc cggcaccggc acggtcgata
     3901 tcagcgtcgt tgcgggctcg gtcgacttcg gccacgcgaa gctcaacgag cacgtgaagt
     3961 cgcccgagat gctcgacgtg cagaagtacc cgaccgcggt ctacaagggc acgctcacca
     4021 atttcaagaa cggcgccccg accgaagtgc acggcgagct gacgctgcac ggcgtgacca
     4081 agccgatgaa cctcaagatc aacagcttcg tgtgcaagtt gacgccgctc tcaaagaagg
     4141 agcgctgcgg cgccgatgca tccggttcct tcgaccgcgc cgatttcggc gtgaactatg
     4201 gcgacaagta cggcttcaac atgcaggtga agctcgcgat ccaggtggag gcggctcccg
     4261 agggctgagc gcacgccggc cgtctatcag accagctgcg ccacgtgcgc cgcaatcgcg
     4321 agcgaggcgg tcaggcc
//
LOCUS       DATLKL010000636         1749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_30280, whole genome shotgun sequence.
ACCESSION   DATLKL010000636 DATLKL010000000
VERSION     DATLKL010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30280"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VM713_08600"
     CDS             complement(<1..1087)
                     /locus_tag="VM713_08600"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HVH84360.1"
                     /translation="MNDSVSTAAPLAPYAAHDERSRGRRHPEPAPELRREFQRDRDRI
                     IHSNAFRRLVYKTQVFVNHEGDLYRTRITHSIEVAQIARSAALALSLNEALTEAISLA
                     HDLGHTPFGHAGQDALNECMRDYGGFEHNLQSLRVVDELEERYAEFRGLNLTFECREG
                     ILKHCSVRHARELGELGERFLTRQQPGLEAQIANLADEIAYNNHDVDDGLRAQLLTVE
                     ALRATRMFRRQHEAVIARYPDLSERRLVYKTQVFVNHEGDLYRTRITHSIEVAQIARS
                     VAVALDVNEALSEAICLAHDLGHTPFGHAGQDALNECMRDYGGFEHNLQSLRVVDELE
                     ERYAEFRGLNLTFECREGILKHCSVRHA"
     gene            complement(1097..>1749)
                     /locus_tag="VM713_08605"
     CDS             complement(1097..>1749)
                     /locus_tag="VM713_08605"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HVH84361.1"
                     /translation="NHAGGKNLIGAFHQPVAVVSDIATLGTLPPRELRAGLAEVIKYG
                     LMCDAALFDWLEAHLDELLAGKDEALAHVVRRSCEIKAIVVARDEREERGDRALLNLG
                     HTFGHAVESATGYRKWLHGEAVGAGLVMAAALSRELGLVTPEELVRVHALIERAGLPT
                     RGAGVAPEAVLEHMRIDKKVLGGRLRLVLLRRIGNAFMTADYSEDALKRTLQAYCG"
BASE COUNT          263 a          593 c          610 g          283 t
ORIGIN      
        1 gcgcgtggcg caccgagcag tgcttgagga tcccttcgcg gcactcgaac gtgaggttga
       61 ggccgcggaa ttcggcgtag cgctcctcga gctcatcgac cacgcgcagc gactgcaggt
      121 tgtgctcgaa gccgccgtag tcgcgcatgc actcattgag cgcgtcctgg ccggcgtggc
      181 cgaagggcgt gtggccgaga tcgtgcgcca ggcagatggc ttcgctcagc gcctcgttca
      241 catcgagcgc caccgccacc gagcgcgcga tctgcgccac ctcgatcgag tgcgtgatgc
      301 gcgtgcggta gagatcgccc tcgtggttga caaagacctg ggtcttgtag accaggcgcc
      361 gctcgctcaa gtccgggtag cgcgcgatga ccgcctcgtg ctgacggcgg aacatgcgcg
      421 tcgcgcgcag cgcctccacc gtgaggagct gcgcccgaag gccgtcatcg acgtcgtgat
      481 tgttgtaggc gatctcgtcg gcgaggttgg cgatctgcgc ctcaagaccc ggctgctggc
      541 gcgtcaggaa ccgttcgccg agctcgccga gctcgcgcgc gtggcgcacc gagcagtgct
      601 tgaggatccc ttcgcggcac tcgaacgtga ggttgaggcc gcggaattcg gcgtagcgct
      661 cctcgagctc atccaccacc cgcagcgact gcaggttgtg ctcgaagccg ccgtagtcgc
      721 gcatgcactc gttgagcgcg tcctggccgg cgtgcccgaa gggggtgtgc ccgaggtcgt
      781 gcgcgagtga gatcgcctcg gtgagcgcct cgttgagact aagcgcgagc gccgcggagc
      841 gcgcgatctg cgcgacctcg atcgagtgcg tgatgcgcgt gcggtagaga tcgccctcgt
      901 ggttgacaaa gacctgggtc ttgtagacca ggcgccggaa ggcattcgaa tggatgatgc
      961 ggtcgcggtc gcgctggaat tcgcggcgca gctccggtgc gggctccgga tggcgccgcc
     1021 cgcgcgaacg ctcatcgtgc gcggcgtagg gcgcgagcgg cgcggcggtg gacacagagt
     1081 cgttcacctc acagcctcag ccgcagtacg cctggagcgt ccgcttaagc gcgtcctcgg
     1141 agtagtccgc ggtcataaac gcgttgccga tacggcgcaa cagcaccagg cgcagccgcc
     1201 caccgaggac tttcttgtcg atgcgcatgt gctcgagaac cgcctccggg gcgacgcccg
     1261 cgccgcgggt cgggagcccc gcgcgctcga tgagcgcgtg cacgcgcacc agctcctcgg
     1321 gagtgacgag cccgagctca cgcgaaaggg ccgccgccat caccagtccc gctcccaccg
     1381 cttccccgtg cagccacttg cggtagccgg tcgccgactc gaccgcgtgt ccgaaggtgt
     1441 ggcccaggtt gagcagcgcc cggtcgccgc gctcctcccg ctcgtcgcgg gcgacgacta
     1501 tcgccttgat ctcgcaggag cggcgcacta cgtgcgcgag cgcctcgtcc ttgccggcga
     1561 gcagctcgtc gagatgcgcc tcgagccagt cgaagagcgc tgcatcgcac atgaggccgt
     1621 atttgatcac ctcggcgagc ccggcgcgca gctcccgcgg cggcagcgtc ccgagcgtcg
     1681 cgatgtcgga gaccaccgcc accggctgat ggaaggcgcc gatcagattc ttgccgcccg
     1741 cgtggttga
//
LOCUS       DATLKL010000637         1878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_188338, whole genome shotgun sequence.
ACCESSION   DATLKL010000637 DATLKL010000000
VERSION     DATLKL010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188338"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VM713_08610"
     CDS             complement(<1..297)
                     /locus_tag="VM713_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HVH84362.1"
                     /translation="MTAVVSAFLLTFAGLFPIVNPLESAPFFLGLTAGLSREERQQLA
                     RKAATNGFALLLGSMALGPWLLEVLGIELPVVRIAGGLVVTALGWKLLSAEELSE"
     gene            450..1301
                     /locus_tag="VM713_08615"
     CDS             450..1301
                     /locus_tag="VM713_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVH84363.1"
                     /translation="MNGKTCLVTGATHGIGLVTARALAAAGATVLVHGRDAELGAAVA
                     ADIARASGNSAVRFVRADFMHLDEVRDLAERLARLPRLDVLVNNAGLMDGVRRARRCT
                     AEGYDATFGVNHLAPFLLTNLLLGKLTQSAPARVVVVASEAHRRARIDFGDLMNARVS
                     FLRAYQRSKLANLLFVRALAARLRGSGVTVNAAHPGFVASELFRSCPPPLRLLLATLA
                     RPFMRSATRGARTSVYLATSPEVEGQSGGYYIDCRRVSPSAAARCDEAAARLWSESAR
                     LTALAHA"
     gene            1298..1762
                     /locus_tag="VM713_08620"
     CDS             1298..1762
                     /locus_tag="VM713_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HVH84364.1"
                     /translation="MSRAFTRESDDDPGTLPERPVSSHPNLVTPSGRAALESQLRSLE
                     SELVAARAGGDSAQRARIERDLRYFAQRLASARVIEPMAAPDRVRFGVQVTLRNAGGV
                     EQSFRLVGEDEADAARGLLSWVSPLGQALLGHEPGDTVRFQAGELEILRLAP"
BASE COUNT          254 a          679 c          672 g          273 t
ORIGIN      
        1 ctcggacaac tcctcggcgc tcagcagctt ccagccgagc gccgtcacga ccaggccgcc
       61 ggcgatgcgc accaccggga gctcgatacc taacacctcg agcagccacg ggccgagcgc
      121 catcgacccg agcagcagcg cgaagccgtt ggttgcggcc ttgcgcgcca gctgttgccg
      181 ctcctcgcgc gacaggcccg cggtgaggcc gaggaagaac ggcgcagatt ccagtgggtt
      241 gacgatgggg aagagtcccg cgaaggtcag caggaacgcg cttacgaccg cggtcatggc
      301 ggcgcggccg cgagcagcgg gtcgatgagc gtcccaacgg gccgcgacgt gccgtcgccg
      361 ctggccaagg tgcgcgcagc gactctcagg gcgcgcatgc tctccgatct ttcatatacc
      421 atcggatctt cgaggggctc gtgcgcagta tgaacggcaa aacctgcctc gtcaccgggg
      481 cgacccacgg gatcgggctc gtgaccgcgc gcgccctggc cgcggcgggc gctaccgttc
      541 tggtgcacgg ccgcgacgcg gagctcggcg ccgccgtggc ggcggatatc gcgcgcgcga
      601 gcggcaactc cgcggtgcgc ttcgtgcgcg cggacttcat gcacctcgac gaggtccgcg
      661 acctggctga gcggctcgcg cggctgccgc ggctcgatgt cctcgtcaat aacgccggcc
      721 tcatggacgg tgtacgccgc gcgcggcgct gcactgccga gggctatgac gccaccttcg
      781 gcgtcaatca cctggcgccc tttctgctca cgaacctgct gctcgggaag ctcacgcaat
      841 cggcacccgc gcgcgtggtc gtggtcgcct ccgaggcaca ccggcgcgcg cggatcgact
      901 tcggcgatct catgaacgcg cgcgtctcct tcctgcgtgc ctaccagcgc tccaagctcg
      961 cgaacctcct gttcgtgcgg gcgctcgccg cgcgcctgcg ggggagcggc gtcaccgtga
     1021 acgcggcgca tccgggcttc gtcgcgagcg agctctttcg cagctgcccg ccgccgctcc
     1081 ggctgctgct cgccaccctc gcccggccct tcatgcgctc ggccacgcgc ggcgcccgca
     1141 ccagtgtgta tctggcgacc tcgcccgagg tggagggcca atccggcggc tactacatcg
     1201 actgccgtcg ggtgtccccg agcgcggcgg cccgttgcga cgaggcggcc gcgcgactgt
     1261 ggagcgagag cgcgcgcctc acggcactcg cgcacgcatg agccgggcgt tcacgcgcga
     1321 gtcggacgac gatccgggga cgctgccgga gcggccggtc agcagccacc cgaacctcgt
     1381 gaccccgagc ggacgcgccg ccctcgagtc gcagctgcgc agcctggaga gtgagctggt
     1441 ggcggcgcgc gccggcggcg acagtgcgca gcgcgcgcgc atcgagcgcg acctgcgtta
     1501 cttcgcgcag cggctcgcga gcgcgcgcgt catcgagccg atggccgctc ccgaccgcgt
     1561 gcgcttcgga gtgcaggtca cgctgcgcaa cgcaggcggc gtcgagcagt cgttccgcct
     1621 ggtaggcgag gacgaggcgg acgcggcgcg cgggctcctc tcctgggtgt cgccgctcgg
     1681 tcaggcactg ctcggccacg agcccggcga cacggtgcga tttcaggctg gtgagctcga
     1741 gatcctgcgc ctcgcgcctt gagccgcgcg cgtggtccct tcagaaatgc cgcgataggt
     1801 acagtacgcc gctcgcgatc ttgtagaagc tgtcgggccg gggtccctgg gggtcggtcg
     1861 gcagcacgtg gtcgcggg
//
LOCUS       DATLKL010000638         1709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_188347, whole genome shotgun sequence.
ACCESSION   DATLKL010000638 DATLKL010000000
VERSION     DATLKL010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188347"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /gene="gshA"
                     /locus_tag="VM713_08625"
     CDS             <1..1395
                     /gene="gshA"
                     /locus_tag="VM713_08625"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HVH84365.1"
                     /translation="HITTDFSESLIELVTPPFTHTWELLQYLLDLHQFVYRHLGDELL
                     WATSMPCAIDGDADIPLAQYGSSHVGRMKTVYRNGLGLRYGRMMQAISGVHFNYSFPL
                     PFWEAYAALRGERSSGSEFISASYFDLLRSYRRHGWLVLYLFGVSPVVCKSFLRGRDV
                     QLEELGMGTAYEPWATSLRMSDVGYRNRNQAGLTVSVNNLADYVRDLQRAITTPHPPY
                     EALGVKVNGEWRQLNANVLQIENEYYSFIRPKRVARSGERPTKALKRAGVEYVEVRAL
                     DVSAFDPVGVNQHKLRFLEAFLALCLLKDSPPIGIEEQAALDANHLTVARRGREPGLT
                     LERDGRKVPMRAWARELLDSMSGICEILDRGDAARPYTQALAMQAAKVADASLTPSAR
                     LLSELASGQSFFDLALRVSETHKAYFLDLYPPNAERLNELAGEAQESLAAQRRIEASD
                     RGTFDEYLARYFAD"
BASE COUNT          306 a          562 c          542 g          299 t
ORIGIN      
        1 cacatcacca ccgacttctc cgagtcgctg atcgagctgg tgacaccccc tttcacccat
       61 acctgggagc tgctgcagta tctcctcgac ctgcaccagt tcgtctaccg ccatctcgga
      121 gatgagctgc tgtgggcgac cagcatgcca tgcgcgatcg acggcgacgc cgacatccca
      181 ctcgcccagt acggcagctc gcacgtcggc cgcatgaaga cggtgtatcg caatggcctg
      241 gggctgcgct acggccgcat gatgcaggcg atctcgggcg tgcatttcaa ctactcgttc
      301 ccgctgccgt tctgggaggc ctatgccgcg ctgcgcggcg agcgctcgag cgggagcgag
      361 ttcatctcgg cgagttactt cgatctgcta cgcagctatc gccggcacgg ttggctcgtg
      421 ctgtacctct tcggggtctc gccggtggta tgcaagtcgt tcctgcgggg acgcgacgtg
      481 cagctcgagg agctcggcat gggtaccgct tatgagccct gggccacgag tctgcgcatg
      541 agcgatgtgg gctaccgcaa ccgcaatcag gcggggctca ccgtctcggt gaacaacctt
      601 gcggactacg tgcgcgacct gcagcgcgcg atcaccacac cgcacccgcc ttatgaggcc
      661 ctcggcgtca aggtgaacgg cgaatggcgg cagctgaacg ccaacgtcct gcagatcgag
      721 aacgagtact acagcttcat ccgtcccaag cgcgtggcgc gctccggcga gcggcccacc
      781 aaggctctga agcgcgcggg tgtcgaatac gtcgaggtgc gcgccctcga tgtcagcgcc
      841 ttcgaccccg tgggggtgaa tcagcacaag ctgcgcttcc tcgaggcgtt cctggcgttg
      901 tgcctgctca aggacagccc gcccatcgga attgaggagc aggccgcgct cgatgcgaac
      961 catctgaccg tggcacgccg tggccgcgag cccgggctca ccctcgagcg cgatggcagg
     1021 aaggtgccga tgcgcgcatg ggctcgggag ctcctcgact cgatgtcggg tatctgcgag
     1081 atcctcgatc ggggtgatgc cgcccggccc tacacgcagg cactcgccat gcaggccgcc
     1141 aaggtcgccg acgcctcgct cacgccgtcg gcacgactcc tgagcgagct cgcctccggg
     1201 cagtcgtttt tcgatctggc gctgcgtgtg tcggagacgc acaaagcgta cttcctcgat
     1261 ctctaccccc ctaacgctga gcgtctgaat gagctcgccg gagaggcgca ggaatccctc
     1321 gcggcccagc ggcggatcga ggcgagcgac cggggaacgt tcgatgagta cctcgcccgg
     1381 tattttgccg attgaagagg ctcagactgg ccaaacggcg tccccgggcg gctgggcgca
     1441 agaccccagg ggccacctcc ccctgtagcg ctccccaatg attacttttg caatccgtga
     1501 cgtcattgca caagccggct acctagaatt gcccaccctg tcgcggttag gcgacaggtt
     1561 aaaggaataa caagagggct attaaggccg atgaatcaag agactgcgca aggactagcc
     1621 gttgagcccc gcgaggacac tgcggatggc aacgaggaat tcaccggggt cgtaagccgc
     1681 ctcgatgccg accggcgcgc gagcggatg
//
LOCUS       DATLKL010000639         2827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_86733, whole genome shotgun sequence.
ACCESSION   DATLKL010000639 DATLKL010000000
VERSION     DATLKL010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86733"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /gene="truB"
                     /locus_tag="VM713_08630"
     CDS             <1..891
                     /gene="truB"
                     /locus_tag="VM713_08630"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HVH84366.1"
                     /translation="GILLLDKPLGLSSNAALQRVRRLLAGAKAGHAGSLDPLATGMLP
                     ICIGEATKIAADIVAGAKRYQFTVALGRRTATADAEGEVTAEAAVPALTRTAVEQLLQ
                     GFLGRRSQVPPMYSALKVSGEPLYKLARSGRTVERPARAIELYELTLLELEPQSLDIE
                     TRCSKGTYVRVLAEEIAAALGTVGHVSALRRLSVEPFGEEPMHTLESLEQTCAAGGAA
                     PLLPADYPLKHLPAVHLAALAAARLRQGQVVPAVVAAARPSSGEAPRLRLYDEAGRFL
                     GVGIVAGAGAVRARRLLNPD"
     gene            complement(888..1133)
                     /locus_tag="VM713_08635"
     CDS             complement(888..1133)
                     /locus_tag="VM713_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84367.1"
                     /translation="MLRPILLGGCLSLALAACASTGTVPPPRAAAPSGLPLGCVPNAQ
                     TQRCDQFGTYYSGDDLRATGAQHVGQALILLDPQLGH"
     gene            complement(1138..1413)
                     /locus_tag="VM713_08640"
     CDS             complement(1138..1413)
                     /locus_tag="VM713_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84368.1"
                     /translation="MIAMLKSFAIGACLVLLSACASQPTSPNKATTAAAKKPPAGCVA
                     QTATRIPVKDDACAGFGNTYTKQDVDDTGQVSLDRALPMLDPAITRH"
     gene            1621..1890
                     /gene="rpsO"
                     /locus_tag="VM713_08645"
     CDS             1621..1890
                     /gene="rpsO"
                     /locus_tag="VM713_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HVH84369.1"
                     /translation="MALTSEKKGGILAQFRRGPADTGSPEVQVALLSERINDLGEHFT
                     AHKRDHASRRGLVKLVNQRRKLLDYLRATQPQKYQELVERLGLRR"
     gene            1970..>2827
                     /locus_tag="VM713_08650"
     CDS             1970..>2827
                     /locus_tag="VM713_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HVH84370.1"
                     /translation="MSVVSKSFPYGPHTVTIETGEVARQAEGAVRVSMGDTVVLVTAC
                     AQQTPAPGRDFLPLTVNYVEKTYAAGRIPGGFFKREGRPTEKETLTSRLIDRPIRPLF
                     PDGFYNEVQVVATVLSMDPEIDADIPALLGASAALALAGIPFNGPIGAARVGYRDGQY
                     LLNPTAKDLAQSQLHLVVAGTEHAVLMVESEAKGLSEQVMLGAVVYGHEQMQVAIGAI
                     KALASEAGKERWKWEPAPENAELTQAVTLQAQAALGQAYSITEKQQRHARLTEIKTQT
                     LNALAAGEQP"
BASE COUNT          472 a          944 c          979 g          432 t
ORIGIN      
        1 gggattctgc tgctcgataa gccgctgggg ctttcgtcga acgccgcgct gcagcgggtg
       61 cgacggctcc tcgcgggcgc gaaggcgggc cacgccggca gtctcgaccc cctcgccacc
      121 ggcatgctgc cgatctgcat cggcgaagcc accaagatcg ccgccgacat cgtcgcgggc
      181 gccaagcgct atcagttcac cgtggccttg ggtcggcgga ccgcaactgc ggatgccgag
      241 ggcgaggtga cggccgaggc ggccgtgccg gcgctgaccc gcaccgctgt cgagcagcta
      301 ctccagggct ttctcggtcg tcgcagccag gtgccgccga tgtactcggc gctcaaggtg
      361 agcggggagc cgctttacaa gctcgcgcgc tccggcagga cggtcgagcg tccggcgcgt
      421 gccatcgagc tatacgagct cacgctcctc gagctcgagc cgcagtcgct cgacatcgaa
      481 acccgctgct cgaagggaac gtacgtgaga gtgctggctg aggagatcgc cgcagcgctc
      541 ggcaccgtgg gacacgtgag cgcgctccgg cgcctctccg tcgagccctt tggcgaggag
      601 cccatgcaca cgctcgagtc gctcgagcag acttgcgcgg caggtggcgc ggcaccgctt
      661 ctgcccgccg attatccgct caagcacctc ccggccgtgc atctcgcggc gctggctgcg
      721 gcgcggcttc gccagggtca ggtggtcccg gcggtcgtgg cggcggcgcg gccgtcctcg
      781 ggcgaggcgc cgcggctgcg cctctatgat gaggctggaa gattcctggg cgtcggcatt
      841 gtggccggtg ccggcgcggt gcgggcgcgg cggctgctca atcccgacta gtgacccagc
      901 tgcggatcga gcaggatgag cgcctgtccg acgtgctgcg cgccggtagc tcgcagatca
      961 tccccgctgt agtaggtgcc gaactgatcg cacctctgtg tctgcgcatt gggcacgcag
     1021 cccagcggca gtccgctcgg ggcggcagcc cgcggaggtg gcacggtccc ggtgctggcg
     1081 caggcggcga gagcgagcga gagacagccg ccgaggagga tcggcctcag cacgacgtca
     1141 gtgtcgcgtg atcgccgggt cgagcatcgg cagtgcccga tcgagggaga cctgacccgt
     1201 gtcgtccacg tcctgcttcg tataggtatt cccgaagccg gcgcacgcgt cgtctttcac
     1261 cggaatgcgc gttgcagtct gcgcgacgca gcccgcaggg ggtttcttgg ccgccgccgt
     1321 ggtggccttg ttcggagagg ttggctgtga ggcgcaggcc gagagcagga cgaggcaggc
     1381 gccgatggcg aaggatttca gcatggcaat cacctcgatt aaaagcgtat gcccgcgggt
     1441 agtgcgctgc aagagaccag gaacgggaga ggcctctctt ggttgacaag ttcgcgggcg
     1501 tggggttccg cgcgggcgcc gtcgggcaga acacccaagc ttgtcccctg gacgttcgga
     1561 cagtacaatg cgcggctcac caaaatggct cttaagacaa gtagttagga ctcaagcgca
     1621 atggctttaa ccagcgagaa aaagggcggg atcctcgcgc agttccgccg cgggccggcc
     1681 gacaccggct cgcccgaagt acaggtggcg ctgctctccg agcgaatcaa cgacctcggc
     1741 gagcacttca ccgcgcacaa gcgcgaccac gcctcgcggc gcggcctggt gaaactggtc
     1801 aaccagcgtc gcaagctcct cgactacctc agagccaccc aaccgcagaa gtaccaggag
     1861 ctcgtcgagc gcctgggact gcgccgctga gaatgcgtaa gggccgccgt cacggacgcg
     1921 cggccctcgt cgttttcggc cgaacctcca actttcgagg aatgaagctt tgagcgtcgt
     1981 cagcaagtcg ttcccttacg gtccgcacac ggtcaccatc gagacgggcg aggtcgcccg
     2041 ccaggccgag ggtgcggtgc gcgtttccat gggcgatacc gtggttctgg tcaccgcctg
     2101 tgcacagcag accccggcgc cgggacggga tttcctcccg ctcaccgtca actacgtcga
     2161 gaagacctac gccgcggggc gcatacccgg cggcttcttc aagcgcgagg gccgtccgac
     2221 cgagaaggag acgctcacct cacgcctcat cgatcgcccg atccgcccgc tcttcccgga
     2281 cggcttctac aacgaggtgc aggtcgttgc gaccgtgctg tcgatggatc cggagatcga
     2341 cgcagacatc ccggcgctgc tcggcgcctc ggccgcgctc gcgctcgcag gcataccttt
     2401 taatggtccg atcggcgcgg cgcgcgtcgg ctaccgcgac gggcagtatc tgctgaaccc
     2461 caccgccaag gacctcgccc agtcacagct gcacctggtg gtagcgggca ccgagcacgc
     2521 ggtgctgatg gtcgagtcgg aggccaaggg gctgtccgaa caggtcatgc tgggcgcggt
     2581 ggtctacggc cacgagcaga tgcaggtggc gatcggcgcc atcaaggcgc tcgcgagtga
     2641 agcgggcaag gagcgctgga agtgggagcc ggcgccagag aacgccgagc tcacgcaggc
     2701 cgtcaccctg caggcgcagg cagccctcgg gcaggcctac agcatcaccg agaagcagca
     2761 gcggcacgcg cggctcacgg agatcaagac ccagacgctg aacgcactcg ccgccggcga
     2821 gcaaccc
//
LOCUS       DATLKL010000640         2653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_221756, whole genome shotgun sequence.
ACCESSION   DATLKL010000640 DATLKL010000000
VERSION     DATLKL010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221756"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VM713_08655"
     CDS             <1..1265
                     /locus_tag="VM713_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH84371.1"
                     /translation="MVQISMQKSSKLARPSLAAGVALAAVLAAAPLLAADQPAKQTNS
                     PKLAKPLKEAHDDLNAKKYPEAIAKLKESEGIAGKSAYDQHLINDMLAFAYIRTNDYA
                     DAAKAMEAEIEDGFTPPSEQQQKIRALSALHYQLKNYDKAIEFGQRALKGGFGEEETK
                     KIVGQAYYLKGDWKGTLKFEEGLVDSAIKAGATPNSESLQLVLSACVKLEDSACQNKA
                     LEKLVTYYPKPEYWSNLLFGLMKETASSDANTLQTFRSMSEVDVLKSADDYIEMAQLA
                     MDAGSPGEAQRALQRGFDKNVFTDQRNKDKAQRLLDKAKQKSASDQPTLDKTAKEADA
                     APTGAKNAGMGLAYLGYGQYDKAVDEYSKAVTKGGLRNAPETQLMLGIAELKAGHKDD
                     AVKAFKAVKGDPVLEHLASLWTLHARQA"
     gene            1293..1775
                     /locus_tag="VM713_08660"
     CDS             1293..1775
                     /locus_tag="VM713_08660"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVH84372.1"
                     /translation="MAIKAGDRMPTGTFKRMTKEGPKDLSTEELFKGKRVVLFSVPGA
                     FTPTCDAKHLPGFVQLADQIHAKGVDTIACMAVNDVFVMNAWGKASGVGDKVLMLADG
                     NGDYARALGLELDASKFGMGKRGQRFAIIVKDGTATHVDVEEPGQFKVSAAEYVLGQL
                     "
     gene            complement(1805..>2653)
                     /locus_tag="VM713_08665"
     CDS             complement(1805..>2653)
                     /locus_tag="VM713_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HVH84373.1"
                     /translation="EITVAVCAAEPGAVAAQAAELAGVARVLTVANPANLHALAAVLA
                     PQIVALAGPYSHVLGPSTTYGKDLMARVAGLLGAPQVSDIMAIESPTRFRRPVYAGNA
                     ILTVEAEGGVKVVGTVRMASFEAAAGGGAAAIEKAAVSADIPTHTRFISVSAARSDRP
                     DLQTAARVISGGRALGSAEGFEILYSLADKLGAAVGASRAAVDAGYAPNEMQVGQTGK
                     IIAPELYVAVGISGAIQHLTGIKDARTIVAINKDGEAPIFEVADFGLVGDLFQIIPEI
                     EKLVSS"
BASE COUNT          524 a          859 c          869 g          401 t
ORIGIN      
        1 caatggtaca gatcagcatg cagaagtcct caaagctcgc acgcccgtcg ctcgccgcgg
       61 gtgtcgccct ggcggcagta ctcgccgcgg cgcctttgct ggccgcggat cagccggcca
      121 agcaaaccaa cagccccaag ctcgccaagc ctctcaagga ggcccatgac gatctcaacg
      181 ccaagaaata ccccgaggcg atcgccaagc tgaaagagtc ggaaggcatc gcgggcaaga
      241 gcgcctacga tcagcacctg atcaacgaca tgctggcctt cgcctacatc cgcaccaacg
      301 actacgcaga tgcggccaag gccatggaag ccgagatcga ggacggcttc acgccgccga
      361 gcgagcagca gcagaagatt cgcgctcttt ccgcgctcca ctaccagctg aagaactacg
      421 acaaggcgat cgaattcgga cagcgcgcgc tcaaaggcgg cttcggcgag gaggagacca
      481 agaagatcgt cggccaggcc tactacctca agggtgactg gaagggcacg ctgaagttcg
      541 aggagggact ggtcgatagc gccatcaagg ccggcgccac tcccaacagc gaatcgctgc
      601 agctggtgct gagcgcctgc gtgaagctgg aagacagcgc gtgccagaac aaagccctcg
      661 agaagctggt gacctactac ccgaagcccg aatactggtc gaatctcctg ttcggcctca
      721 tgaaggaaac cgcgagcagc gatgccaata ccttgcagac cttccgttcg atgtcggagg
      781 tcgacgttct gaagagcgcc gatgactaca tcgagatggc gcagctggcg atggacgccg
      841 gctcgcccgg ggaggcacaa cgggcgctgc agcgcgggtt cgacaaaaac gtctttactg
      901 accagcgcaa caaggacaag gcgcaacggc tgctcgataa ggcgaagcag aagtcagcct
      961 ccgatcagcc gacgctcgat aagaccgcca aggaagccga cgcggcgccc acgggggcca
     1021 agaacgccgg catgggcctc gcctacctcg gctacggcca gtatgacaag gcggtcgatg
     1081 agtactccaa ggccgtgact aaaggcggcc tgcgtaatgc gcccgagacg cagctgatgc
     1141 tcggcatcgc cgagctcaag gcgggccaca aggacgatgc cgtcaaggct ttcaaggcgg
     1201 tcaagggcga tccggtcctc gagcacctcg ccagcctctg gaccctgcac gcgcgacagg
     1261 cctgagtttc aggctgcgca aaggagcttc ccatggcgat caaggccggc gaccgcatgc
     1321 ccacaggtac gttcaaacgc atgaccaagg agggtccgaa ggacctgtcg acggaggagc
     1381 tcttcaaggg taagcgggtc gtgctcttct ccgtgcccgg cgccttcacg ccgacctgcg
     1441 atgccaagca cctgccaggg ttcgtgcagc ttgccgatca gattcacgcc aagggcgtcg
     1501 acacgattgc ctgcatggcg gtgaacgacg tcttcgtcat gaacgcctgg ggcaaggcct
     1561 ccggagtggg cgacaaggtg ctgatgctcg cggatggcaa cggcgactat gcgcgcgccc
     1621 tgggactcga gctcgatgcg agcaagttcg gcatgggcaa gcgcgggcag cgcttcgcga
     1681 tcatcgtcaa ggacggtacc gccacgcacg tggacgtgga ggagcccggc cagttcaagg
     1741 tgtcggccgc cgagtacgtg ctcggacagc tatagcgcga gagtctcgct cgagcctttc
     1801 ggctctagct gcttacgagt ttctcgatct cggggatgat ctggaagagg tccccgacga
     1861 gtccgaaatc cgccacttcg aagatcggcg cctcgccgtc cttgttgatg gcgacgatgg
     1921 tgcgcgcgtc cttgatgccg gtgaggtgct ggatcgcgcc ggagatgccg accgccacgt
     1981 acagctccgg ggcgatgatc ttgccggtct ggcccacctg catctcgttc ggcgcgtagc
     2041 cggcatcgac cgcagctcgt gaggcaccga ccgcagctcc cagcttgtcg gccaggctat
     2101 agaggatctc gaacccttcg gcgctcccga gtgcccggcc gccggagatg acccgcgcgg
     2161 cggtctgcag atccggccgg tcgctcctcg ccgcggacac cgagatgaag cgcgtgtgag
     2221 tggggatgtc cgctgacacg gccgccttct cgatggcagc cgcgccgccg cccgccgccg
     2281 cctcgaacga cgccatgcgc accgtgccga cgaccttgac gccgccctcg gcctccaccg
     2341 taaggatcgc gttgccggca tagacggggc ggcggaagcg ggtggggctc tcgatggcca
     2401 tgatgtcgct cacctgaggc gcgccaagga gcccggcgac gcgcgccatc aggtccttgc
     2461 cataagtggt cgagggtccg agcacgtggc tgtagggacc ggcgagcgcc acgatctgcg
     2521 gtgcgagcac cgcggcgagc gcgtgcaggt tggcgggatt ggcgaccgtg agcacgcgcg
     2581 caactccggc gagctctgcc gcctgcgcgg cgactgcacc cggctccgcg gcgcatacgg
     2641 cgacggtgat ttc
//
LOCUS       DATLKL010000641         3068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_176877, whole genome shotgun sequence.
ACCESSION   DATLKL010000641 DATLKL010000000
VERSION     DATLKL010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176877"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2698
                     /gene="carB"
                     /locus_tag="VM713_08670"
     CDS             <1..2698
                     /gene="carB"
                     /locus_tag="VM713_08670"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HVH84374.1"
                     /translation="GGGIGYNREELEDIVARGLDASPTGEVLLEESVIGWKEFEMAVV
                     RDHRDNCIIICSIENLDPMGVHTGDSITVAPALTLTDKEYQRMRDASVAVLRKIGVDT
                     GGSNVQFAVNPRDGRLLIIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDELKN
                     DITGGATPAYFEPTIDYVVVKIPRFTFEKFPAANDRLTTQMKSVGEVMAIGRTFQESL
                     QKALRGLETGLDGLTPQLALPLTDETQAKLAHELRAPGANRLLYVADAFRAGWTRERV
                     AELTYIDPWFLAQVEELVREEAEVSANGLGALTVERLRALKRRGFSDSRLARLLDMPE
                     KAIRDKRHELGVVPVYKRVDTCAAEFATSTAYLYSTYEEECEANPTDRRKIMILGGGP
                     NRIGQGIEFDYCCVHAALQLREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVL
                     EVLAKEKPEGVIVQFGGQTPLKLSRALEAAGAPIIGTSPDSIDIAEDRERFQALVRKL
                     GLKQPANATARTEDQAVVLAREVGFPLVVRPSYVLGGRAMEVVFNEEDLRTYTTNAVQ
                     VSNDSPVLLDRFLDVAIEVDVDAVCDGEDVLIGGIMEHVEQAGVHSGDSGCSLPPNSL
                     SRETQNELREQTRKLARALNVVGLMNVQYAIQAGVIYVLEVNPRASRTVPFVSKATGM
                     PLAKVAARVMTGRKLKQLVGAVERRPHYFSVKEAVFPFNKFPEADPILGPEMKSTGEV
                     MGTGRTFGEAYAKAQSASGVTLPRKGVCFVSVRDRDKPGAVELAKSLIERGFEIVATE
                     GTARALTEAGVACRRVNKVREGRPHIVDMIKNDEIDLIVNTTEGKLAIRESNSIRREA
                     VHRRVTYYTTLAAGLATLEALDHMDEVEVNRLQDLHLEASGV"
     gene            2695..>3068
                     /gene="greA"
                     /locus_tag="VM713_08675"
     CDS             2695..>3068
                     /gene="greA"
                     /locus_tag="VM713_08675"
                     /inference="COORDINATES: protein motif:HMM:NF001264.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HVH84375.1"
                     /translation="MKRTPMTLRGAEVLRVELKRLKSEARPAIIKQIAEARGHGDLSE
                     NAEYHAAREQQGFIEGRITEIERKLGNAEVIDPATLPNTGKVVFGATVELEDQAGGAR
                     VVYQIAGEDDADIRAGRISITSP"
BASE COUNT          543 a         1034 c         1031 g          460 t
ORIGIN      
        1 cggcggcggc atcggctaca accgcgagga gctcgaggac atcgtggcgc gcggcctcga
       61 cgcctcccca accggcgagg tgctgctcga ggagtcggtg atcggctgga aagaattcga
      121 gatggcggtg gtgcgcgacc accgcgacaa ctgcatcatc atctgctcga tcgagaacct
      181 ggacccgatg ggggtgcaca ccggggactc gatcaccgtc gccccggccc tcacgcttac
      241 cgacaaggaa taccagcgca tgcgcgacgc ctcggtcgcg gtgctgcgca agatcggcgt
      301 cgacaccggg gggtccaacg tccagttcgc cgtcaacccg cgcgacgggc ggctgctgat
      361 catcgagatg aatccgcgcg tgtcgcgctc ctcggcgctc gcctccaagg cgaccggctt
      421 tccgatcgcc aagatcgccg ccaagctcgc cgtcggctac acgctcgatg agctgaagaa
      481 cgacatcacc ggcggcgcga ctccagccta cttcgagccg accatcgact acgtggtggt
      541 gaagattccg cgcttcacct tcgagaagtt ccccgcggcc aacgaccgcc tcacgaccca
      601 gatgaagtcg gtcggtgagg tcatggcgat cggccgcacc ttccaggagt cgctgcagaa
      661 ggcgttgcgc ggcctcgaga ccggactcga tgggctcacg ccgcagctcg cgctgccgct
      721 caccgacgag acccaggcga agctcgcgca cgagctgcgg gccccggggg cgaaccggct
      781 gctgtacgtt gccgacgcct tccgcgccgg ctggacgcgc gagcgcgtcg cggagctcac
      841 ctacatcgat ccctggttcc tcgcccaggt cgaggagctg gtgcgtgagg aggccgaggt
      901 tagcgccaat ggtctcgggg ccttgaccgt cgagcggttg cgggcactca aacgccgcgg
      961 cttctccgac agccgcctgg cgcggttgct cgacatgccg gagaaggcca tccgcgacaa
     1021 gcgccacgag cttggcgtcg tgcccgtcta caagcgggtc gacacctgtg cggccgagtt
     1081 cgccacctcc accgcgtacc tctattcgac ttacgaggag gagtgcgagg cgaatcccac
     1141 cgaccgccgc aagatcatga ttctcggcgg cggccccaac cgcatcggcc aggggatcga
     1201 gttcgattac tgctgcgtgc atgcagcgct gcagctgcgc gaggacggct tcgagaccat
     1261 catggtcaat tgcaaccccg agaccgtctc gaccgactac gacacctccg atcggctcta
     1321 cttcgagccg ctcacgctcg aggacgtgct cgaggtgctc gccaaggaga aacccgaggg
     1381 ggtcatcgtg cagttcggcg ggcagacgcc gctgaagctc tcgcgggcgc tggaggccgc
     1441 cggcgcgccg atcatcggca cctcgccgga ttcgatcgac atcgccgagg accgcgagcg
     1501 cttccaggcg ctggtgcgga agctcggcct gaagcagccc gccaacgcga ccgcgcgcac
     1561 cgaggaccag gccgtggtgc tcgcgcgcga ggtgggcttt ccgctcgtgg tgcggccttc
     1621 ctacgtgctc ggcgggcgcg ccatggaagt cgtcttcaac gaggaggacc tgcgcaccta
     1681 tacgactaac gccgtacagg tatcgaatga cagtccggta ctgctcgacc gctttctcga
     1741 tgtcgccatc gaggtggacg tggacgcggt gtgtgacggc gaggacgtcc tgatcggcgg
     1801 gatcatggag cacgtcgagc aggccggcgt gcactcgggc gattctggct gctcgcttcc
     1861 tcccaacagc ctgtcgcgcg aaacccagaa cgagctgcgc gagcagacgc gcaagctcgc
     1921 gcgcgcgctc aatgtcgtcg gcctgatgaa cgtgcagtac gccatccagg cgggcgtcat
     1981 ctacgtgctc gaggtgaacc cgcgtgcctc gcgcaccgtg ccgttcgtct ccaaggccac
     2041 gggcatgccg ctcgccaagg tcgcggcgcg ggtcatgacg gggcgcaagc tcaagcagct
     2101 ggtcggcgcg gtggagcgcc gaccccacta cttctccgtg aaggaagcgg tgttcccgtt
     2161 caacaagttc ccggaagccg atccgatcct cggacccgag atgaaatcga cgggtgaggt
     2221 catgggcacg ggacgcactt tcggcgaggc ttatgccaag gcgcagagcg cctcgggtgt
     2281 gacgctgccg cgcaaggggg tgtgcttcgt cagcgtgcgc gaccgtgaca agccgggcgc
     2341 ggtcgagctc gcgaagagcc tcatcgagcg cggctttgag atcgtcgcga ccgagggcac
     2401 ggcgcgcgcg ctcaccgagg cgggcgtcgc ctgccgccgc gtcaacaaag tgcgcgaggg
     2461 ccggccgcac atcgtggata tgatcaagaa cgacgagatc gatctcatcg tcaacaccac
     2521 cgagggcaag ctcgccatcc gcgagtcgaa ctcgatccgg cgcgaggccg tgcaccggcg
     2581 cgtcacgtac tacacgacgc tcgcggccgg actcgcgacg ctggaagcgc tcgaccacat
     2641 ggacgaggtt gaggtcaacc gtctgcagga cctgcacctc gaggcatccg gcgtatgaag
     2701 cgcaccccca tgacgctgcg cggcgccgag gtgctgcgcg tggagttgaa gcgcctcaag
     2761 tccgaggcgc ggcccgcgat catcaagcag atcgccgagg cgcgcggaca cggcgatctc
     2821 tccgagaacg ccgagtatca cgcggcgcgc gagcagcagg gattcatcga ggggcgcatc
     2881 accgagatcg aacgcaagct cggtaacgcc gaggtcatcg acccggcgac cctgccgaac
     2941 accggcaagg tggtattcgg cgccacggtg gagctcgagg accaggcggg cggcgcccgc
     3001 gtcgtttatc agattgcggg cgaggacgac gccgacatac gcgccggacg catctcgatc
     3061 acctcgcc
//
LOCUS       DATLKL010000642         1543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_52793, whole genome shotgun sequence.
ACCESSION   DATLKL010000642 DATLKL010000000
VERSION     DATLKL010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52793"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..>1543
                     /locus_tag="VM713_08680"
     CDS             15..>1543
                     /locus_tag="VM713_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVH84376.1"
                     /translation="MDKIWLKAYPPYVPATIDPSKLRSLKQLLEETCAKYADRVAYIY
                     MGATITYGELDTLLRAFGAWLQQQGFRKGDRIAIMLPNSPQYPIAMFGALRAGLIVIN
                     TNPLYTAPELEHQLADAGATAIVVLENFAHVVEKVLPHTQVKKVLVTTVGDFLSFPKS
                     AIVNFVVRRVKKQVQPWRIASATSFKEAVNAGRKVPLAPVDLGPDDLAFLQYTGGTTG
                     VAKGAMLSHGNVSANVLQAEAWIGKSLPDHGTILTAIPLYHIFALSCNCMVFARLGWQ
                     NLLIINPRDLKSLIADMQRYPCNFISGVNTLFNAMLHAPGFDKIDFSGLKISLGGGMA
                     VQAAVAAKWKQATGNVLSQAWGLTETSPAACINPPTEEFNGSIGVPIPSTEIAIKDDA
                     GNDLELGKIGEICVRGPQVMKGYWNRPDETAKVMLPDGYLRTGDVGHMDERGFVFIED
                     RKKDMILVSGFNVYPNEVEAVAAANPGILEAAAVAQPDEHSGEVVALFVVRKDPALTE
                     KG"
BASE COUNT          292 a          516 c          477 g          258 t
ORIGIN      
        1 accgggggta gctgatggac aagatctggc tgaaggcgta cccgccgtat gtacccgcga
       61 ctatcgatcc ctcgaagctg cgctcgctca agcagctgct cgaggagacc tgcgccaaat
      121 acgccgaccg ggtcgcctac atctacatgg gcgcgacgat cacttacggt gagctcgata
      181 cactcttgcg cgccttcggc gcgtggctgc agcaacaggg tttccgcaag ggcgaccgca
      241 tcgcgatcat gctgcccaac agcccgcagt acccgatcgc catgttcggc gccctgcgcg
      301 ccgggctcat cgtcatcaac accaatccgc tctacacggc gcctgagctc gagcatcagc
      361 tcgccgatgc cggggcgacg gccatcgtgg tgctcgagaa cttcgcccac gtggtggaaa
      421 aggtcctgcc ccatacccag gtgaagaagg tgctcgtcac gacggtcggg gattttttat
      481 ccttccccaa gtccgcgatc gtcaacttcg tcgtgcggcg ggtgaagaag caggtgcagc
      541 cgtggcgcat cgcgagcgcg acttcgttca aggaagcagt gaatgcaggc aggaaggtcc
      601 cgctggcacc cgtcgacttg ggtcctgacg acctggcgtt ccttcagtac acgggcggca
      661 cgaccggggt cgccaagggt gcgatgctct cgcacggcaa cgtgagcgcc aacgtgctgc
      721 aggccgaggc ctggatcggc aagagcctgc cggaccacgg caccatcctc accgcgatcc
      781 ccctctatca catctttgcg ctctcctgta actgcatggt gttcgcgcgc ctcgggtggc
      841 agaacctcct catcatcaat ccgcgcgacc tgaaatccct gatcgccgac atgcagcgct
      901 acccttgcaa cttcatctcg ggcgtcaaca cgctcttcaa cgcgatgctg cacgccccgg
      961 ggttcgacaa gatcgatttc agcgggctca agatctcgct cggcggcggc atggcggtgc
     1021 aggcggcggt cgcggcgaaa tggaagcaag cgaccggcaa cgtactctcg caggcctggg
     1081 gcctcaccga gacctcgccc gccgcctgca tcaatccgcc caccgaggag ttcaacggct
     1141 cgatcggggt gccgatcccc tccaccgaaa tcgccatcaa ggacgatgcc ggcaacgacc
     1201 tcgagttagg aaagatcggc gagatctgtg tccgcggccc gcaggtgatg aagggctact
     1261 ggaaccgccc agacgagacc gccaaggtga tgctgccgga cgggtacctg cgcaccggcg
     1321 acgtcggcca catggacgag cgcggcttcg tcttcatcga ggaccgcaag aaagacatga
     1381 tcctcgtctc gggcttcaac gtgtacccga acgaggtcga ggccgtggcc gccgccaatc
     1441 ccgggatcct cgaggccgcc gcggtcgcgc agcccgacga gcactcgggc gaggtggtgg
     1501 ccttattcgt ggtccgcaag gatccggcgc tcaccgagaa ggg
//
LOCUS       DATLKL010000643         2171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_64267, whole genome shotgun sequence.
ACCESSION   DATLKL010000643 DATLKL010000000
VERSION     DATLKL010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64267"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VM713_08685"
     CDS             <1..223
                     /locus_tag="VM713_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84377.1"
                     /translation="ERALEVAERLAHGPASAYGATKRLIAHSLGAFETQMVLESETIA
                     TQATSPDAAEGIGAFLEKRKPRFPNKGPR"
     gene            253..648
                     /locus_tag="VM713_08690"
     CDS             253..648
                     /locus_tag="VM713_08690"
                     /inference="COORDINATES: protein motif:HMM:NF018574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HVH84378.1"
                     /translation="MMSFVAALAVALVLIVVRVYFKLRAAHRARVAGWDAQLIERLRA
                     RGYVPFNDYPVDFFLALPSESSCQDVRARLEPEFSVDVKAMQDEAEFPFSLHATKSMR
                     LILPDMQALSARMTALAAEFHGRYDGWAA"
     gene            complement(651..1400)
                     /gene="gpmA"
                     /locus_tag="VM713_08695"
     CDS             complement(651..1400)
                     /gene="gpmA"
                     /locus_tag="VM713_08695"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HVH84379.1"
                     /translation="MVGTLILVRHGQSTWNLENLFTGWTDVDLTEQGRVEAAQAGREL
                     LKERLDIGIAFTSVLKRAIRTLWIMLDEMDRMWVPVERSWRLNERHYGALQGLDKAQT
                     VARHGEAQVKIWRRSYDMPPPALAADDERHPRFDPRYAGVAPQELPATESLKDTLARV
                     LPCWHARIAPELRSGRNVLVVAHGNSLRAMVKMLDQVSESEIVELNIPTGVPLLYELD
                     ADLKPRGSRYLGDPQAIAAAAEAVRRQTEKR"
     gene            complement(1403..>2171)
                     /locus_tag="VM713_08700"
     CDS             complement(1403..>2171)
                     /locus_tag="VM713_08700"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84380.1"
                     /translation="EDTGAEPAQIARAYTAAREIFAMREVWEQIEALDNRVPAKLQYE
                     AAFQTSRLLRHATYWLLTARRRGLQVDAAVKEFRSGVRELEVRSAELLTGDELERFGE
                     ARRRYAEAGLPPQLAARIASLEALNAALDIVEISAAHGVSVTETARVYFEVGTRTGID
                     WLRTRIEKLAVDGPWQAIARTGLRDGALRVHRRLTERVLSRHERGSASARVGAWMEAA
                     GKELAHWQRTLTDMRAAGATDFATLTVGVETVRKLSN"
BASE COUNT          306 a          777 c          749 g          339 t
ORIGIN      
        1 cgagcgcgcc ctcgaggtgg ccgagcgcct ggcgcacgga ccggccagcg cctacggcgc
       61 gaccaagcgc ctgatcgcgc actcgctcgg cgccttcgag acgcagatgg tcctcgagag
      121 cgagaccatc gccacgcagg ccacgagtcc cgacgccgcg gaaggcatcg gcgcgtttct
      181 cgagaagcgc aagccgcgct ttcccaacaa gggcccgcgc tgagcggggc ggaccttaca
      241 ggcaacgctc ccatgatgtc cttcgtcgcg gcgctggcgg tggccctggt gctcatcgtg
      301 gtgcgcgtgt acttcaagct gcgcgccgcg caccgcgccc gcgtggcggg ctgggacgcg
      361 cagctcatcg agcggctgcg cgcgcgcggc tacgtgccct tcaacgacta tccggtcgat
      421 ttcttcctcg cgctgcccag cgagtcatcc tgccaggacg tgcgcgcgcg cctcgagccg
      481 gagttcagcg tcgacgtcaa ggctatgcag gacgaagccg agtttccctt cagcctccac
      541 gccaccaaga gcatgcggct gatcctgccc gacatgcagg cactgagtgc gcgcatgacg
      601 gcgctcgcgg ccgagttcca cgggcgctac gacggctggg ccgcctgaag tcagcgcttc
      661 tcggtctgac gcctgacggc ttcggcggcg gcagcgatcg cctgcgggtc gccgaggtag
      721 cggctgccgc gcggcttcaa atccgcatcg agctcgtaga gcagcggcac gccggtcggg
      781 atgttgagct cgacgatctc gctctcgctc acctgatcga gcatcttgac catggcgcgc
      841 aggctgttgc cgtgcgccac gaccagcacg ttgcgccctg agcgcagctc cggcgcgatg
      901 cgcgcatgcc agcagggcag cacgcgcgcc agagtgtcct tgagggactc cgtcgccggc
      961 agctcctggg gagcgacgcc ggcgtaccgc ggatcgaagc gcggatggcg ctcatcgtcg
     1021 gccgcgaggg ccggcggcgg catgtcgtag ctgcgtcgcc agatcttcac ctgggcctcg
     1081 ccgtgccggg cgacggtctg cgccttgtcg agcccctgca gcgctccata gtggcgctcg
     1141 ttgagccgcc acgagcgctc gaccggcacc cacatgcggt ccatctcatc gagcatgatc
     1201 cacagcgtgc gaatggcgcg cttcagcact gaagtgaagg cgatgccgat gtcgagccgc
     1261 tccttgagga gctcgcggcc cgcctgcgcc gcctcgaccc ggccctgctc ggtcagatcg
     1321 acgtcggtcc agccggtgaa gaggttctcg agattccagg tgctctggcc gtgacggacc
     1381 aggatcagcg taccaaccat ggtcagttcg agagcttgcg gaccgtctcg acgcccaccg
     1441 tcagcgtggc gaaatcggtg gcgccagccg cgcgcatgtc ggtgagcgtg cgttgccagt
     1501 gggcgagctc cttgccggcc gcctccatcc acgcgccgac gcgcgcgctg gcgctgccgc
     1561 gctcgtggcg gctcagcacc cgctcggtga ggcggcggtg cacgcgcagc gccccgtcgc
     1621 gaagtccggt gcgcgcaatc gcctgccacg gcccgtcgac ggcgagcttc tcgatgcgcg
     1681 tgcgcagcca gtcgatgccg gtgcgtgtcc ccacctcgaa atagacgcgc gccgtctccg
     1741 tgacgctcac gccgtgcgcc gcggagatct cgacgatgtc gagcgccgca ttgagcgcct
     1801 cgaggctggc aatgcgcgcg gcgagctgcg gcggcagccc cgcctcggca tagcgtcggc
     1861 gcgcttcgcc gaagcgctcg agctcatccc cggtgaggag ctcggcgctg cgcacctcga
     1921 gctcgcgcac gccgctgcgg aattccttta ccgccgcgtc gacctgcagg ccacgccggc
     1981 gcgcggtgag cagccagtag gtggcatggc gcagcaggcg gctggtctgg aaggcagcct
     2041 cgtactggag cttggcgggc acgcggttgt cgagcgcctc gatctgctcc cacacctcgc
     2101 gcatggcgaa gatctcacgc gccgcggtat aggcgcgggc gatctgtgcg ggctccgcac
     2161 ccgtgtcttc c
//
LOCUS       DATLKL010000644         1955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_98260, whole genome shotgun sequence.
ACCESSION   DATLKL010000644 DATLKL010000000
VERSION     DATLKL010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98260"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VM713_08705"
     CDS             <1..477
                     /locus_tag="VM713_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84381.1"
                     /translation="LKQLPHDAETEAYVGVAGPLVGTVGALGCFYLARETGSTLLLAL
                     AYSGFFLNLFNLIPLAPFDGGRVTAAISPRLWLIGAPLLVALFFYSPSPLLILMALLA
                     LPNVLKAWRGVSSPEEQAYYRVPAETRVSYAALYLGLAAFLAIMCGQLHAELPRGH"
     gene            complement(474..1475)
                     /locus_tag="VM713_08710"
     CDS             complement(474..1475)
                     /locus_tag="VM713_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HVH84382.1"
                     /translation="MSPPEPAPSDAALESLLAAARERIRTRHLSLRTEQVYLRWMQRY
                     AAFHGGRLLRELGPRDIETFLTHLAVSEHVGPATQNQALQALLFLYRQVLGIELPWLE
                     NVTRATHVKRLPVVLSRVEVRSLLARLDGTSWLIASLLYGSGLRLMEGMRLRVKDLAL
                     ERGELIVREGKGSKDRVTMLPAALVTPLRAHLTRLRAWFEEERRRDQPGVSLPYALAR
                     KYPQAGTQWGWQYLFPAPTLCRDPYRGVPVRHHLYGKRVQRAVQQAVRRAGLTQPASC
                     HTLRHCFATHLLEDGYDIRTVQELLGHSDLKTTMIYTHVMTKGARGVRSPLDRPDRP"
     gene            1630..>1955
                     /locus_tag="VM713_08715"
     CDS             1630..>1955
                     /locus_tag="VM713_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84383.1"
                     /translation="MTWWGWVIAGAILLGAELLFVSAQFYLVFVGSAAILVGLAALTL
                     PLAEWLQWGLFAVLAIVSMVTFRNRIYQRFRVPTPALETGPQGGLITLPAALGAGDSC
                     QAEHRG"
BASE COUNT          287 a          690 c          646 g          332 t
ORIGIN      
        1 ctgaagcagc tgccgcacga cgccgagacg gaagcctacg tcggtgtcgc aggcccgctc
       61 gtgggcacgg tcggcgcgct cggctgtttc tatctcgcgc gcgagaccgg cagcacactg
      121 ctgctcgcgc tcgcctactc gggcttcttt ctcaatctct tcaacctcat tccgctcgcg
      181 cccttcgacg gcggccgggt cacggcagca atctccccac ggctgtggct gatcggggcg
      241 ccgttgctcg tcgcgctctt tttctacagc ccgagcccac tgctcatcct catggcgctg
      301 ctcgcgctgc cgaacgtcct caaggcctgg cgcggcgtct cgagcccgga agagcaggcc
      361 tactaccgcg tgcccgccga gacgcgcgtt tcttacgccg cgctctacct cggactcgcc
      421 gcatttctcg cgatcatgtg cgggcagttg cacgctgagc tgccgcgcgg ccattagggc
      481 cggtcggggc ggtcgagggg gctgcgcacc ccacgcgcgc ccttcgtcat cacgtgcgtg
      541 tagatcatgg tggttttcag atccgagtgg ccgagcagct cctgcacggt gcggatgtcg
      601 tagccgtcct cgagcaggtg cgtcgcgaag cagtgacgca acgtgtgaca gctcgccggc
      661 tgcgtgagcc cggcgcggcg caccgcctgc tgcacggcgc gctgcacgcg cttgccgtac
      721 aggtgatgtc gcaccgggac cccgcgatag ggatcgcggc acagggtcgg cgccgggaag
      781 agatactgcc agccccactg cgtgccggcc tgcgggtact tgcgtgccag cgcatacggg
      841 agcgacaccc ccggctggtc gcggcgccgc tcttcctcga accaggctcg cagccgtgtc
      901 aggtgtgcgc gcagtggcgt cacgagcgcc gcgggcagca tcgtgacccg atccttgctg
      961 cccttgccct cgcgcacgat gagctcgccc cgctcgagcg cgaggtcctt gacgcgcagc
     1021 cgcatccctt ccatcagacg caggccgctg ccgtacagga ggctcgcaat cagccacgag
     1081 gtgccatcga gccgcgcgag gagcgagcgc acctcgacgc ggctcagcac gaccggcagg
     1141 cgcttcacgt gggtcgcgcg cgtcacgttc tcgagccacg gcagctcgat tccgagcacc
     1201 tgacggtaga ggaacagcaa cgcctgcagc gcctgattct gtgtcgcggg acccacgtgc
     1261 tcgctgactg cgagatgcgt gaggaaggtc tcgatatcgc gcgggccgag ctcgcggagt
     1321 agccggccgc catgaaaggc cgcatagcgc tgcatccagc gcaggtacac ctgctcggtg
     1381 cgcagcgaca aatggcgcgt acggatccgc tcgcgcgctg cggccaggag actctccagc
     1441 gccgcgtcag agggggcagg ctcgggcggg gacatacagc aatccgttcc gtgatggaac
     1501 cagagccacg cggcgcccgc cagcggcgaa agacgcgcgt ttgccgcatt tcagacacgc
     1561 aagccgttat aattttgccg gtcgccgccc gggccgcggc cgcgcacaat cagcttacgg
     1621 tgacctcaga tgacctggtg gggctgggta atcgccggcg caatcctcct cggcgccgaa
     1681 ctgttgttcg tcagcgcgca gttctacctg gtattcgtcg gtagcgccgc gatcctggtc
     1741 ggtctcgcgg ccctgacgct gccgctcgcg gagtggctgc agtggggtct gttcgcggta
     1801 ctcgccatcg tgtccatggt gactttccgc aaccgcatct atcagcgctt ccgcgtcccg
     1861 acgccagcgc tcgagactgg tcctcagggc ggtctcatca ccctcccggc cgctctcgga
     1921 gccggcgaca gctgccaggc ggagcaccgc ggcag
//
LOCUS       DATLKL010000645         1746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_132018, whole genome shotgun sequence.
ACCESSION   DATLKL010000645 DATLKL010000000
VERSION     DATLKL010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132018"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..>1746)
                     /locus_tag="VM713_08720"
     CDS             complement(61..>1746)
                     /locus_tag="VM713_08720"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HVH84384.1"
                     /translation="ATLAPVQRRVIEHAVRDFRLAGVALPAEQKSRFKTVMQELTQLQ
                     AKFEENVLDAANAWSWHVTDPDELRGLNELVIELAARRARERGVSGWLLTLDQPTYVA
                     VLTDAESASLRRAFYEAWATRASDRGPGAGRWDNSSVMEEILRRRHEAARLLDFRNYA
                     EYALATRMARSVPEVLEFLHQLARSARPAALAEFAELEDFAHAPLAAWDVGFHSERLQ
                     RARFAVSQEELRPYFPLPRVLGGLFAVAERLFGVCIRERAGVPAWHQDVRFFDLESAA
                     GVPLGSFYLDAYARPNKRSGAWMDECIGRKRLGSGAALPVAYLVCNFLPPGAGRPALL
                     THDDVVTLFHEFGHGLHHLLTRVDYPSLAGINGVAWDAVELPSQFFENYAWHPEVLQQ
                     ISGHFTSGAALPREMQSRLIATRSFQAGLAMMRQLEFALFDFRMHAEYSPGRGARVLE
                     ILAEVRREVAVVPVPEWNRFAHSFGHIFAGGYAAGYYSYKWAEVLAADAFAAFEESGV
                     FDRGTAQRFLDAILSRGGSRDALEAFIEFRGRTPDVRPLLKQHGIVAAGGLAA"
BASE COUNT          270 a          627 c          625 g          224 t
ORIGIN      
        1 gcggggcctg ggcgtgcgcc gcgagcgcgc acgagagcag tcccacggcg agcgcgcgcc
       61 tcaagcggcc agcccgcccg cggccacgat gccgtgctgc ttgaggagcg ggcgcacgtc
      121 gggtgtccgg ccgcgaaact cgatgaaggc ctcgagcgca tcgcggctgc cgccccggga
      181 gaggatcgca tcgaggaagc gctgcgccgt gccgcgatcg aacacgccgc tctcctcgaa
      241 ggcggcgaac gcgtccgccg ccagcacctc ggcccacttg tagctgtagt agccggcggc
      301 atacccgccc gcgaagatgt ggccgaagct gtgcgcgaag cgattccact ccggcaccgg
      361 cacgacggcg acctcgcggc gcacctcggc gaggatttcg agcacccgcg cgccgcgccc
      421 gggcgagtac tccgcgtgca tgcggaaatc gaacagcgcg aattccagct gccgcatcat
      481 cgcgagcccc gcctggaagc tgcgcgtcgc gatgaggcgc gactgcatct cgcgcggcag
      541 cgccgcgcca ctcgtgaagt ggcccgagat ctgctgcaga acctccgggt gccacgcata
      601 gttctcgaag aactggctcg gcagctccac cgcatcccag gctacgccgt tgatgccggc
      661 aaggctcggg tagtcgaccc gggtgaggag gtggtgcagt ccgtgcccga actcgtgaaa
      721 cagcgtcacc acgtcgtcgt gggtgagaag cgcaggtctc cccgcgcccg gcggcaggaa
      781 attgcacacc aggtacgcga ccggcagggc cgcccccgag ccgaggcgct tgcgcccgat
      841 gcactcgtcc atccaggcgc cgctgcgctt gttggggcgt gcgtaggcgt cgaggtagaa
      901 gctgccgagc ggcacgccgg cggcgctctc gaggtcgaag aagcgcacgt cctggtgcca
      961 cgccgggacc ccggcgcgct cgcggataca cacgccgaag agccgctcgg cgaccgcgaa
     1021 cagcccgccg agcacccgcg gcagcggaaa gtagggccgc agctcctcct gcgacacggc
     1081 gaagcgcgcg cgctgcagcc gctcggagtg gaagcccacg tcccaggccg cgagcggcgc
     1141 gtgcgcgaag tcctcgagct cggcgaactc ggcgagcgcg gcggggcggg ccgagcgcgc
     1201 cagctggtgc aggaactcga gcacctcggg gacgctgcgc gccatacggg tcgccagcgc
     1261 gtactcggcg taattccgaa agtcgaggag ccgcgcagcc tcgtgccgcc gccgcaggat
     1321 ctcctccatt acggaggaat tgtcccagcg gcccgcgccc ggcccgcggt ccgaggcgcg
     1381 cgtggcccac gcctcgtaga aagcgcggcg caggctcgcg gattcggcgt ccgtcagtac
     1441 ggcgacgtag gtcggctggt cgagggtaag gagccagccg ctcacgcccc gctcccgcgc
     1501 gcggcgcgcg gcaagttcga tgaccagctc gttgaggccg cgcagctcgt ccgggtcggt
     1561 gacgtgccag ctccaggcgt tggccgcgtc cagcacgttc tcctcgaatt tcgcctgcag
     1621 ctgcgtgagc tcctgcatca cggtcttgaa tcggctcttc tgctcggccg gcagggcgac
     1681 gccagcgagg cggaagtccc gcacggcgtg ctcgatgacc cggcgctgca ccggcgcgag
     1741 cgtggc
//
LOCUS       DATLKL010000646         2753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_221815, whole genome shotgun sequence.
ACCESSION   DATLKL010000646 DATLKL010000000
VERSION     DATLKL010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221815"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VM713_08725"
     CDS             complement(<1..208)
                     /locus_tag="VM713_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84385.1"
                     /translation="MTSARLSPRGLAAGAAAFTIWGLFPVYLHPLSGVPALQIIAHRV
                     AWSCLLLLGVLLWRAELGVLTTTLG"
     gene            complement(240..1226)
                     /gene="epmA"
                     /locus_tag="VM713_08730"
     CDS             complement(240..1226)
                     /gene="epmA"
                     /locus_tag="VM713_08730"
                     /inference="COORDINATES: protein motif:HMM:NF006828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HVH84386.1"
                     /translation="MKAGDDWRPSATLEALRARAALLARAREFFAARSVLEVDTPLLV
                     NSPVSDVHIASAAVELGAAPSAAGPAPPLYLHTSPEYAMKRLLAAYSRDIFQICHVVR
                     GNERGRLHNPEFTLIEWYRIGFSLGALMDEVEALVRLLLGPAGAERRSERLSYREAFA
                     RELGLDPFSAPDSTLEEAAHRLGLKSRGGSRDALLEFLMGVSVGPRLGRGALTFIHGY
                     PATQAALARLDPEDPRAALRFELYCEGVELANGFHELESASEQRARFERDNEERRRTG
                     QPVRPPDERLLAALAAGLPDCAGVALGLERTLMLALGATHIDAVLSFPTARA"
     gene            complement(1223..2098)
                     /gene="asd"
                     /locus_tag="VM713_08735"
     CDS             complement(1223..2098)
                     /gene="asd"
                     /locus_tag="VM713_08735"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HVH84387.1"
                     /translation="MRSDDTGARLFVGLQYLLPQHLLSGVVHWLARLHLKWVKDALID
                     NFVVNYRPDLSEAEEPDPRRYATFNAFFTRALRPGVRPIDPAPAALVSPVDGTVSQIG
                     RTEGSSLVQAKGLTYTLESLLDEAAPAWAPVFRGGAFATLYLAPHNYHRIHMPLGGTL
                     QAAWYVPGQLFSVNATTAAQVPGLFARNERVVCVFAQASLSFALGLVGALFVGSISTV
                     WHGDVTPRSPRVRGALALDFVRAGLALEKGAEMGRFNMGSTVILLLPPGRCEWLGELA
                     TGSVIRVGQALARLT"
     gene            complement(2095..>2753)
                     /locus_tag="VM713_08740"
     CDS             complement(2095..>2753)
                     /locus_tag="VM713_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HVH84388.1"
                     /translation="NARSAVTAVRRPWFWILVVVAAAGAALLGFLLAQQLDRESPALA
                     SGTWLPRPKPLGDFALVDASGRPYTRADLTSAPTLVYFGFTHCPDVCPTTLAKLAQLR
                     RQPGLDSLRVLFVSIDPQRDSPAAVGLYAHAFDPSFQGVTGKPAEIESLARNFAVAVN
                     RVDLPGGDYTMDHSAVVFLVAANGIVAIFTPPFEVPALAADLRRAAAYLAHAPGGRPT
                     "
BASE COUNT          441 a          940 c         1037 g          335 t
ORIGIN      
        1 ggccgagcgt cgtcgtgagc acgccgagct cagcgcgcca gagcagcacg cccaggagca
       61 gcaggcacga ccaggcgacg cggtgcgcga tgatctgcag cgcgggtacg ccgctcaagg
      121 ggtggagata gaccgggaac agtccccaga tcgtgaaggc ggcggccccg gccgcgagcc
      181 cgcggggcga gagccgggcg gaggtcatga gcggcgcacc gcggctgcgc gcatgccgct
      241 cacgcgcgcg ccgtcggaaa gctgagcacc gcgtcgatgt gcgtggcccc gagcgccagc
      301 atcaaggtgc gctcgagccc gagcgccacc ccggcgcagt ccgggagccc ggctgcgagc
      361 gctgcgagga gccgctcgtc gggcgggcgc acgggctgcc cggtgcgccg ccgctcctcg
      421 ttgtcgcgct cgaagcgcgc gcgctgctcg gatgccgact cgagctcatg gaagccgttg
      481 gcgagctcga cgccctcgca gtagagctcg aagcgcagcg ccgcccgcgg atcttccggg
      541 tcgaggcgcg cgagcgcggc ctgagtcgcg ggatagccgt ggatgaaggt cagggcgccg
      601 cgacccaggc gcgggccgac gctgaccccc atcaggaact cgagcagcgc gtcgcggctg
      661 ccgccgcggg acttgagccc gaggcggtgc gcggcctcct cgagagtcga gtccggggcg
      721 ctgaagggat cgaggccgag ctcgcgcgcg aacgcctcgc ggtagctcaa gcgctcgctg
      781 cggcgttccg cgccggcggg gcccagcagc aggcgtacca gcgcctcgac ctcgtccatg
      841 agagcgccga gcgagaagcc gatgcggtac cactcgatga gcgtgaactc cgggttgtgc
      901 agccgcccgc gctcgttgcc gcgcaccacg tggcagatct ggaagatgtc gcgggagtaa
      961 gcggcgagta agcgcttcat cgcgtactcg ggcgaggtgt ggaggtagag cggcggcgct
     1021 gggcccgcgg ccgacggcgc cgcgccgagc tcgaccgccg cgctcgcgat gtgcacatcg
     1081 cttacaggcg agttgaccag gagcggagta tccacctcga gcacggagcg cgcggcgaag
     1141 aattcccggg cgcgcgcgag cagcgcggcg cgcgcgcgca gcgcctcgag cgtggcgctt
     1201 ggccgccagt cgtcgccggc cttcatgtga gccgtgccag ggcctggccc acgcggatga
     1261 cgctgccggt ggcgagctcg ccgagccact cgcaccggcc gggcggcagc agcaggatca
     1321 ccgtcgatcc catgttgaag cgccccatct ccgcgccctt ctcgagcgcg agcccggcgc
     1381 gcacgaagtc gagcgcgagg gcgccgcgca cgcgcgggct gcgcggcgtg acatcgccat
     1441 gccacaccgt cgagatgctg ccgacgaaca gcgccccgac cagccccagc gcgaaggaga
     1501 ggctagcctg cgcgaacaca cacaccacgc gctcattgcg ggcgaagagc cccggcacct
     1561 gggcggcggt ggtcgcattc acgctgaaga gctggcccgg cacgtaccag gcggcctgca
     1621 gcgttccgcc gagcggcatg tggatgcgat ggtagttgtg cggggcgaga tagagagtcg
     1681 cgaatgcgcc gccgcgaaac accggcgccc aggcaggcgc ggcctcatcg aggagcgact
     1741 cgagggtata ggtgagtccc ttggcctgca ccagggatga gccctcggtc cgtccgatct
     1801 gactcaccgt gccgtcgacg ggcgagacca gtgccgcggg cgcggggtcg atgggccgca
     1861 cgcccgggcg cagcgcgcgc gtgaagaagg cgttgaaggt cgcgtaccgg cgcggatccg
     1921 gctcctccgc ttcgctcagg tcggggcgat agttgacgac gaaattgtcg atgagcgcgt
     1981 ccttcaccca cttaagatgc agccgcgcga gccagtgaac gacgcccgac agcaggtgct
     2041 gcggcaagag gtactgcagc cccacgaaga gccgcgcgcc ggtgtcgtcg ctcctcacgt
     2101 ggggcgcccg cccggcgcgt gcgcgaggta ggccgccgcc cggcgcaggt ccgccgcgag
     2161 ggcgggcacc tcgaaggggg gcgtgaagat cgcgacgatc ccattcgcag ccactaagaa
     2221 gacgacggcg gaatgatcca tggtgtagtc gccgcccggg aggtcgacgc ggttgacggc
     2281 caccgcgaag ttccgcgcga ggctctcgat ctctgcgggc ttaccggtga caccctgaaa
     2341 ggagggatcg aacgcatgcg cgtagagccc gaccgccgcc gggctatcgc gctgcgggtc
     2401 gatgctgacg aacagcacgc gcagggagtc gaggcctggc tgacggcgga gctgcgcgag
     2461 cttcgccagc gtcgtcggac agacgtccgg gcagtgcgtg aacccgaagt acacgagcgt
     2521 cggcgcgctc gtcagatccg cgcgcgtgta ggggcgcccg cttgcgtcca ccagcgcgaa
     2581 gtcgccgagc ggcttcgggc gcggcagcca cgtgccgctt gccagcgcgg gagattccct
     2641 gtcaagttgc tgcgcgagga ggaagccgag gagcgccgcg ccagcggccg cgacgacgac
     2701 gaggatccaa aaccaaggtc ttcgcacggc ggtcacggcg ctccgggcgt tca
//
LOCUS       DATLKL010000647         1941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_41751, whole genome shotgun sequence.
ACCESSION   DATLKL010000647 DATLKL010000000
VERSION     DATLKL010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41751"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /gene="istA"
                     /locus_tag="VM713_08745"
     CDS             <1..1367
                     /gene="istA"
                     /locus_tag="VM713_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVH84389.1"
                     /translation="AALEARLFSAVGTKQGHRRHAEPEWAEVHRELKRKHVTLQILWD
                     EYIELCPDGYRYSRFCELYRSWASRLSVTMRQSHSGGDKLFVDYAGDTVPVIIDRLNG
                     KTRPAQIFVAVLGASNFTYAEASWTQGLGDWIAAHTRAFAAIGGVPRLLVPDNTKVAV
                     IKACLYEPQVNRSYAEMAAHYDTAILPARPRRPRDKAKVEAAVLIIERWLLGRLRHRR
                     FYSLTELNAAIGELLRQLNDERPLRRLGVTRRALLEELDRPNLKGLPAEPYCFAEWRL
                     RRVGIDYHIEVDAHFYSVPYRFARHEVEVRLTPQTVEIFLKGERIAAHRRGSGNHGHT
                     TLAEHMPSSHRRYADWTIERIRHTAASIGPATAALCELILERRPHPEQGFRACLGIVR
                     LVRPFGAAGLEAAATRAIEIGTLTYGSVRSILDHKLDRAPPRPPVDDVPLLHANIRGP
                     RYYH"
     gene            1379..>1941
                     /locus_tag="VM713_08750"
     CDS             1379..>1941
                     /locus_tag="VM713_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH84390.1"
                     /translation="MLTHPTLDQLNQLGLPGMAQAFAELETCGDAATLSHADWLGLLL
                     DREVTHRRDKRLAARLRYARLRQHAVIEDVDYRAPRGLDRSLFQKLADGEWIDAHDNL
                     ILCGPTGVGKSWLACALGHKACRDNRSVLYCRVPKLFTDLALARGDGRYARILRSLAG
                     VQLLILDDWGLEPLDAGARHDLYEILE"
BASE COUNT          320 a          696 c          606 g          319 t
ORIGIN      
        1 acgcggcgct ggaggcgcgg ctcttcagcg ccgttggcac caagcaagga catcgccggc
       61 acgctgagcc cgagtgggcc gaagttcatc gcgagctgaa gcgcaagcac gtcaccctgc
      121 agatcctgtg ggacgagtac atcgagctct gtccggacgg gtatcgctat tcgcgcttct
      181 gcgaactgta ccgcagctgg gcgtcgcggc tgtcggtgac gatgcgccag agccacagcg
      241 gcggcgacaa gctgtttgtc gattacgcag gcgacacggt gccggtgatc atcgatcggc
      301 tcaacggcaa gacccggcca gcgcaaattt ttgtcgccgt gctcggcgcc tcgaacttca
      361 cctatgccga agcgagctgg acgcaggggc tcggcgattg gatcgctgcc cacaccaggg
      421 cctttgccgc cattggcggc gtgcccaggc tgctggtgcc ggacaacacc aaggtcgcgg
      481 tcatcaaggc ctgcctttac gagccgcagg tcaatcgcag ctacgccgag atggcggcgc
      541 attacgacac ggcgatcctg ccggcacggc cgcgccggcc gcgcgacaag gcaaaagtcg
      601 aagccgcggt gctgatcatc gagcgctggc tgctgggccg actgcgccat cgccggtttt
      661 acagtctgac cgagctcaat gcggcgatcg gcgagttgct gcggcagctc aacgacgagc
      721 gcccgctgcg acggctcggg gtcacccgcc gcgccttgct cgaagaactc gaccgaccca
      781 acctcaaggg gctgcccgcc gagccctatt gctttgccga atggcgcttg cgtcgcgtcg
      841 gcatcgacta ccacatcgag gtcgacgctc acttttacag cgtcccgtac cgcttcgcgc
      901 gccacgaggt cgaggtgcgg ctgaccccgc agaccgtcga gatctttctt aagggtgagc
      961 ggatcgcggc gcatcggcgc ggcagcggca accacgggca caccaccctg gccgagcaca
     1021 tgccgtcgag ccatcggcgc tatgccgact ggaccatcga acgcattcgc cacaccgccg
     1081 cttcgatcgg tccggccacc gctgcccttt gcgagctgat cctggagcgc cggccgcatc
     1141 ccgaacaagg cttccgcgcc tgtctcggca tcgtccgcct ggttcggccg ttcggcgccg
     1201 ctggccttga agccgcagcg acccgagcca tcgagatcgg caccctgacc tacggctcgg
     1261 tgcgctcgat cctcgatcac aagctcgacc gagcgccgcc gcgaccgccc gtcgacgacg
     1321 tcccgctgct ccacgccaac atccgcgggc cgcgctacta ccattaagga gatccgagtt
     1381 gctcacccac ccgacactcg accagctgaa ccagctcggc cttcccggca tggcgcaagc
     1441 cttcgccgaa ctggagacct gcggcgacgc cgcgacgctc agccatgccg actggctggg
     1501 gttgctgctc gaccgcgagg tcacgcatcg tcgcgacaag cgcctggccg cccgcttgcg
     1561 ctatgcccgg ctgcgccagc acgccgtcat cgaggacgtc gactatcgcg cgccgcgcgg
     1621 tctcgaccgg tccttgttcc aaaagctcgc cgacggtgaa tggatcgacg cgcacgacaa
     1681 tctgatcctg tgcgggccta ccggcgttgg caagagctgg ctggcctgcg cgctcggcca
     1741 caaggcctgc cgcgacaacc gctcggtgct ctattgtcgc gtgcccaagc tgttcaccga
     1801 tctcgccttg gcgcgcggcg acggacgcta tgctcggatc ctgcgcagcc tcgccggcgt
     1861 ccagctgctg atcctcgatg attggggcct tgaacccctc gacgccggcg cccgtcacga
     1921 tctctacgag atcctcgagg a
//
LOCUS       DATLKL010000648         1830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_7579, whole genome shotgun sequence.
ACCESSION   DATLKL010000648 DATLKL010000000
VERSION     DATLKL010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7579"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1524
                     /locus_tag="VM713_08755"
     CDS             58..1524
                     /locus_tag="VM713_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVH84391.1"
                     /translation="MIELKRGSVLAGLLAPISLIAVAAALAGCAVGPNYVKPDTAVAA
                     KFTGADGGPYSADDAQARFWTQFGDATLDRLVDDALGANHDLRIALGRLVEARAIRRQ
                     AQFDLAPTVTAAGGYTRERFSAIESPTGAPFDGHFYDAGFDAFWELDFFGRVRRNVEA
                     QSAEVQGAEASLRDAQVSVTAEVARTYFELRGEQLQLAVARDNVENERQTLQVTEARL
                     EAGSGTELDTSRAQAQLSATLATIGPLQASVARSIHRLSVLTGREPNALTALLGPPHE
                     LPDLPRIAAIGDPAGLLRRRPDIRVAERQLATSTALVGVAIGDLFPKVTFTGSFSYSA
                     PETSALGNSSSRGYVVGPAISWAAFDLGRVRAEVAASRARADVALATYEQTVLRALEE
                     TEDALVTHARTRESLKDATDAAQASLKAARLARTRYEGGVVDFLAVLDAERTQLATED
                     QLTQSRTEAATSLIAVYKALGGGWQMTPLPSYVRQAGG"
BASE COUNT          282 a          635 c          622 g          291 t
ORIGIN      
        1 cggcgcacca tacccaggct gactcgcctg cgttgcttgc ggtcccggag ccctcacatg
       61 attgaactga agcggggcag cgtgctcgcc gggcttctcg cgccgatctc gctcatcgca
      121 gtggccgcgg cgctcgccgg ctgcgccgtg ggacctaact acgtgaagcc cgatacagcg
      181 gtggcggcga agttcactgg cgccgacggc gggccgtact cggccgacga tgcgcaggcc
      241 cggttctgga cacaattcgg cgacgcgacg ctcgaccgtc tcgtcgacga tgcgctcggg
      301 gccaatcacg atctgcgcat cgccctcggg cgcctcgtcg aggcacgcgc cattcgccgt
      361 caggcgcagt tcgacctcgc gccgacggta accgccgccg gcggctatac acgcgagcga
      421 ttctccgcca tcgagagccc gaccggcgcc cccttcgacg gccacttcta cgacgctggg
      481 ttcgacgcgt tctgggagct ggatttcttt ggacgcgtgc gccgcaatgt cgaggcgcag
      541 agcgctgagg tccagggggc cgaggccagc ctgcgcgatg cgcaggtgtc ggtcactgcc
      601 gaggtggcac gcacctactt cgagctgcgc ggcgagcagt tgcagctcgc cgtggcgcga
      661 gacaatgtcg agaacgagcg gcagacgctc caggttaccg aggcgcgact cgaggccggc
      721 agcggcaccg agctcgacac ctcaagggca caggcgcagc tttccgccac gctcgccacc
      781 atcggtccgc tgcaagcgag cgtcgcgcgc tccatccacc gcctcagcgt cctcaccggc
      841 cgtgagccca acgctctgac ggcgctcctc ggacccccgc acgagcttcc agacctgccg
      901 cgcatcgcgg cgatcggcga cccggcgggc ctcctgcgcc gccggccgga tatccgcgtc
      961 gcggagcgcc agctggctac ctccactgct ctcgtgggcg tggccatcgg ggacctcttc
     1021 ccgaaggtaa cgttcacggg cagctttagt tactcggctc cggagacaag tgcgctgggc
     1081 aacagctcga gccgcggcta tgtggtcgga cccgccatct cctgggcggc gttcgacctc
     1141 gggcgtgtgc gcgcggaagt tgcggccagt cgcgcgcgag ccgacgttgc cctcgccact
     1201 tatgagcaga ccgtgctgcg tgcgctggaa gaaaccgagg acgcgctggt gacgcacgcg
     1261 cgtactcgcg aaagcctgaa ggatgcgacc gatgccgctc aagcgagcct caaggccgcc
     1321 cgccttgcgc gcacgcgcta cgagggtggc gtggtcgact ttctggcggt gctcgacgca
     1381 gagcgcacgc agctcgcgac tgaggaccaa cttacccagt cgcgcaccga ggcagcgacc
     1441 agtctgatcg ccgtctacaa ggccctcggg ggcggttggc agatgacgcc gttgccaagc
     1501 tatgtgcgtc aggcgggagg ctgagggcct acgaagttca cgccgagctt ggtcagcttg
     1561 gcctttgggt cgagacgttc acctgggtgg gtgctgcgat gcgccgggaa ggtgcgcggg
     1621 ggcgcgttcg ggcgtaacat cggcccatcg ttgcgccagg agatggccca gcgcagagct
     1681 ccactatggc gccccaggtg tggactacga atacccggga cctcaccctc gatcccgcgg
     1741 ggtaccaggt cgatgacctg accgtcgacc ttgcaccgcg gcgggtgagg cgtgtcggaa
     1801 cggtcatccc cctcaaggcc ctgtctttcg
//
LOCUS       DATLKL010000649         2442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_98287, whole genome shotgun sequence.
ACCESSION   DATLKL010000649 DATLKL010000000
VERSION     DATLKL010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98287"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1441)
                     /gene="accC"
                     /locus_tag="VM713_08760"
     CDS             complement(98..1441)
                     /gene="accC"
                     /locus_tag="VM713_08760"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HVH84392.1"
                     /translation="MIEKLVIANRGEIALRILRACRELGIKTVAVHSTADYSLKHVLL
                     ADESVCIGPPASGASYLNMPAIISAAEVTDSVAIHPGYGFLSENADFAERVEKSGFVY
                     VGPKADTIRTMGDKVSAIKVMKSHGVPCVPGSDGPLGEDSDENLRIARDIGYPVIIKA
                     SGGGGGRGMRVVHTEASLLNAIGITRAEARAAFGNDQVYMEKFLERPRHIEFQVLGDA
                     HGNVIHLGERDCSMQRRHQKVIEEAPAPGISLRQRKLMGDRCVEACRAVGYRSAGTLE
                     FLYEDGEFYFIEMNTRIQVEHSVTELITGIDLVKAQLLIASGERLAYSQSDVQIRGHA
                     IECRINAEDPKTFVPSPGPIRLWHAPGGPGIRVDSHVYSGYNVPPYYDSLIGKVIAHG
                     DNRDTAIARMKNALAEMVIEGIKTNVALHQEIFNHAAFQKGGTDIHYLERRLGLR"
     gene            complement(1449..1916)
                     /gene="accB"
                     /locus_tag="VM713_08765"
     CDS             complement(1449..1916)
                     /gene="accB"
                     /locus_tag="VM713_08765"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HVH84393.1"
                     /translation="MDIRKIKKLIELLEESGIAEIEIKEGEEAVRISRMGAPSAGTVP
                     VTQVMHTAAPTASIPPLQPAALAEGAAAAKPKPNEHVITAPMVGTFYAAPSPGAKAFV
                     EIGDEIKVGQVLCVIEAMKMMNQIEADKAGRITSIMARNGDPVEFGQPLFVIE"
     gene            complement(1962..2408)
                     /gene="aroQ"
                     /locus_tag="VM713_08770"
     CDS             complement(1962..2408)
                     /gene="aroQ"
                     /locus_tag="VM713_08770"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HVH84394.1"
                     /translation="MARVLLLNGPNLNLLGTREPGVYGTETLESIEKRAVALAREAGH
                     ELLPFQSNAEHELVERIQRAPSEGVGFVIFNPGAFTHTSIALRDALLATHVPFIELHL
                     SNTQAREPFRRHSYFSDIAVGVIAGFGAFGYELAVRAAAQRLARGA"
BASE COUNT          389 a          774 c          826 g          453 t
ORIGIN      
        1 ccctcgggat cgagggcacc caggtcgaag gataccgtga cggtcggggc catgagccaa
       61 ttatgcgcgc cgggcgcgtg ctgcaaaccc aaggtcctca ccgcaatccg agccgtcgct
      121 ccagataatg gatgtcggtg ccgccctttt ggaaggcggc gtggttgaag atttcctggt
      181 gcagggcgac gttggtcttg atgccctcga tcaccatctc cgcgagcgca ttcttcatgc
      241 gggcgatggc cgtgtcgcgg ttgtcgccgt gcgcgatcac cttcccgatg agtgagtcat
      301 agtagggcgg cacgttgtag cccgagtaca cgtggctgtc gacgcggata ccggggcccc
      361 cgggcgcgtg ccacaggcgg atcggccccg gcgagggcac gaaggtcttc gggtcctcgg
      421 cgttgatgcg gcactcgatg gcatggccgc ggatctgcac atcgctctgg gagtaagcca
      481 gccgctcgcc gctcgcgatg agcagctgcg ccttgaccag atcgatgccg gtgatgagct
      541 cggttaccga atgctcgacc tggatgcggg tgttcatctc gatgaaatag aactcgccgt
      601 cctcgtagag gaactcgagc gtgccggcgc tgcggtagcc aactgcgcgg caggcttcga
      661 cgcaccggtc tcccatcagc ttgcgttgcc gcaggctgat cccgggggcc ggtgcctcct
      721 cgatcacctt ctggtgccgg cgctgcatcg agcagtcgcg ctcgccgagg tggatgacgt
      781 taccgtgcgc atcgccgagc acctggaact cgatgtggcg cggccgctcg aggaatttct
      841 ccatgtagac ctggtcgttg ccgaaggcgg cgcgtgcctc ggcgcgcgtg atgccgatgg
      901 cgttgaggag cgaggcctcg gtatgcacca cgcgcatgcc gcgcccgccg cccccgcccg
      961 aggccttgat gatgaccgga tagccgatgt cgcgcgcgat gcgcaggttc tcgtccgagt
     1021 cctcgcccag cgggccgtcc gagcccggta cgcacggcac gccgtgcgac ttcatgacct
     1081 tgatcgccga gaccttgtcg cccatggtgc ggatggtgtc cgccttgggt ccgacataca
     1141 cgaagccgct tttttcgacg cgttcggcga agtcggcgtt ctcggaaaga aagccgtagc
     1201 cgggatggat ggcgaccgag tcggtcacct ccgcggcgct gatgattgcc ggcatgttga
     1261 ggtagctggc gcccgatgcc ggcgggccga tgcacaccga ctcatccgcg agcagcacgt
     1321 gcttgagact gtagtcggcg gtggagtgga ccgcgaccgt cttgatgccg agctcgcggc
     1381 atgcccgcag gatccgcagg gcgatctcgc cgcgattggc gatgaccagc ttctcgatca
     1441 tggccacgct attcgatgac gaacaacggt tggccgaact ccaccggatc gccgttccgc
     1501 gccatgatcg aggttatacg tccggccttg tcggcttcga tctggttcat catcttcatc
     1561 gcttcgatga cgcacagaac ctggccgacc ttgatctcat cgccgatctc gacgaacgcc
     1621 ttcgctcccg gcgagggggc ggcatagaag gtgccgacca tgggcgcggt gatgacgtgc
     1681 tcgttgggtt tgggcttggc ggcggccgcc ccttcggcga gtgcggccgg ctgcagcggc
     1741 gggatcgagg ccgtcggcgc agctgtgtgc atcacctgcg taaccggcac agtgcctgcg
     1801 ctgggcgcgc ccatgcggct gatgcgcacc gcctcttcgc cctccttgat ctcgatctcc
     1861 gcgatgccgg actcctccag cagctcgatc agtttcttga tttttcgaat gtccatgccg
     1921 ggtatgcccc caggatcact gcagcgagga ctgtgcgacg ctcaggcgcc gcgggcgagc
     1981 cgctgcgcgg cggcgcgcac cgccagctcg tagccgaagg ccccgaatcc ggcgatgacg
     2041 ccgacagcaa tgtcggagaa ataggaatgg cggcgaaaag gctcgcgcgc ctgcgtgttg
     2101 gaaagatgca gctcgatgaa aggcacgtgc gtcgcgagca gcgcgtcgcg cagcgcgatg
     2161 ctcgtatgcg tgaaggcgcc cgggttgaag atcacgaatc caacgccctc agagggggca
     2221 cgctggatgc gctcgacgag ctcgtgctcg gcgttgctct ggaagggaag gagctcgtgc
     2281 cccgcctcgc gtgccagcgc gacggcccgc ttctcgatgg actcgagggt ctcggtgccg
     2341 taaaccccgg gctcacgggt gccgagcaga ttcagattcg ggccgtttaa caacagcact
     2401 cgggccatcg ggagcgcgtc ttcttatcgg tatgcgggaa ac
//
LOCUS       DATLKL010000650         1802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_41810, whole genome shotgun sequence.
ACCESSION   DATLKL010000650 DATLKL010000000
VERSION     DATLKL010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41810"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VM713_08775"
     CDS             <1..500
                     /locus_tag="VM713_08775"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HVH84395.1"
                     /translation="NKLARAGADFLICPDNTIHQAMPWVLRRSPLPWLHIAEVVAAQA
                     VERGFGTLGITGTRYLVDSEVYPEKLAARGLRYLRPGSAERAEINRIIFDELVCGVFK
                     PAAVAYFQGVIARMKEAGCDAVVLGCTEIPLIIDDANSPLPTLDSTRLLARAALRRAT
                     QRTAS"
     gene            complement(570..791)
                     /locus_tag="VM713_08780"
     CDS             complement(570..791)
                     /locus_tag="VM713_08780"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HVH84396.1"
                     /translation="MAESVYKIVELVGTSTESWEKAAAAAVTQAAKSLRDLRIVEVKQ
                     LDMQVAGGKVRAYRARVNVSFKFESKKEK"
     gene            complement(934..>1802)
                     /gene="rocF"
                     /locus_tag="VM713_08785"
     CDS             complement(934..>1802)
                     /gene="rocF"
                     /locus_tag="VM713_08785"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HVH84397.1"
                     /translation="AALSQRDLEVVDLGNLNGPGNPWQPPRQGYRHLPQVVRWNELVH
                     EAVYGQLGAGRLPILLGGDHSLGIGSISAIARHCREQGRKLRVLWLDAHADFNTSELS
                     PSGNVHGMPVAMLCGFGPRELTTLGGSSPAIDPSWVRQIGIRSVDDGEKRFVHEQGLD
                     VFDMRHLDEMGMRATMEQALAGLDHDTHLHVSFDVDFLDPAIAPGVGTRVPGGPTYRE
                     AQLCMEMIADSRRLASLDVVELNPAFDEHNKTAVLAVDLIESLFGKSTLVRRPVWPHH
                     RAERTARAALAG"
BASE COUNT          271 a          645 c          586 g          300 t
ORIGIN      
        1 cgaacaagct cgcgcgcgcc ggcgccgatt ttctcatctg tccggacaac accattcacc
       61 aggcgatgcc gtgggtgctg cgccgctcgc cgctgccatg gctgcacatc gccgaggtcg
      121 tggccgcgca ggcagtcgag cgcggctttg gcaccctcgg catcacgggc acgcgctacc
      181 tcgtcgacag cgaggtgtat ccggagaagc tcgcggcgcg cggactcagg tacctgcgcc
      241 ccgggagcgc ggagcgtgcc gagatcaacc gcatcatctt cgatgagctg gtgtgcgggg
      301 tgttcaagcc cgcggcggtc gcctacttcc agggcgtcat cgcgcgcatg aaggaggcag
      361 ggtgcgacgc cgtggtgctc ggctgcaccg agatcccgct gatcatcgac gatgccaatt
      421 cgccactgcc gacgctcgat tccacgcggc tgctggcgcg cgcggcactg cgccgcgcaa
      481 cgcagcgcac cgcctcctga cggaacgagt ctcgagccgc gcgcagcggc cgcggcccgg
      541 gcagccgccg cggccgcggg cactcgagtc tatttctctt tcttgctctc gaacttgaag
      601 gacacgttga ccctggcacg gtaggcgcgc accttgccgc ccgccacctg catatcgagc
      661 tgcttgacct cgacgatgcg caggtccctg aggctcttgg ccgcctgagt caccgcggcc
      721 gcggccgcct tctcccacga ctcggtgctc gtgccgacca gctcgacgat cttgtagacg
      781 ctctctgcca tggcttgctc acctttcgcc ccgtcgcggg gactctcttc ttttcacgca
      841 gtgtgcgctt tccgcgggct gcgcgccacg cgacgccctt gaaaccgaag cattggatga
      901 gcactgacga cggctacgac gtcagcagcg acttcagcct gccaatgcag cgcgtgcagt
      961 acgctccgcc ctgtggtgcg gccacaccgg ccggcgcacc agcgtgctct tgccgaacag
     1021 cgactcgatc aggtccaccg cgagcaccgc ggtcttgttg tgctcgtcga acgcagggtt
     1081 gagctccacc acgtcgagcg aggcgaggcg ccgcgaatcc gcgatcatct ccatgcacag
     1141 ttgcgcctcg cggtaggtcg ggccgcccgg gacgcgcgta cccacccccg gcgcgattgc
     1201 ggggtcgaga aaatcgacgt cgaagctcac gtgcaggtgc gtatcgtgat ccagtcccgc
     1261 gagcgcctgc tccatggtcg cccgcatgcc catctcgtcc aggtggcgca tgtcgaatac
     1321 gtcgagcccc tgctcgtgca cgaagcgctt ctcgccgtcg tcgacgctgc ggatgccgat
     1381 ctggcgcacc cagctcgggt caatggcggg gctcgatccg ccgagcgtgg tgagctcgcg
     1441 cggcccgaac ccgcacagca tcgccaccgg catcccatgt acgttgccgc tcggggagag
     1501 ctcgctggta ttgaagtcgg catgcgcatc gagccacagc acgcgcagct tccggccctg
     1561 ctcgcggcag tggcgcgcga tcgcgctgat cgagccgatg ccgagagagt gatcaccgcc
     1621 gagcaggatc ggcagccgtc ccgcgcccag ctgcccgtac acggcctcat gcaccagctc
     1681 gttccagcgc acaacctgcg gcaggtgccg atagccctgg cgaggcggtt gccaggggtt
     1741 cccgggcccg ttgagattgc cgaggtcgac gacctcgagg tcgcgctggg agagcgccgc
     1801 gg
//
LOCUS       DATLKL010000651         2942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_98355, whole genome shotgun sequence.
ACCESSION   DATLKL010000651 DATLKL010000000
VERSION     DATLKL010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98355"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VM713_08790"
     CDS             <1..199
                     /locus_tag="VM713_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84398.1"
                     /translation="GYYYASHPDHHPPRAVDYVYGNAGFALEWRRWRIDVGYFAAQMQ
                     AAELFPYPIAKRIAGTISWQF"
     gene            287..907
                     /locus_tag="VM713_08795"
     CDS             287..907
                     /locus_tag="VM713_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVH84399.1"
                     /translation="MSGYNDMVEVTPRGGSAAAREAELIKRVARHDRRAFEELYNLYH
                     RRLARFLTRLTRRFDLAEEIINDTFLVVWRSAGGFRGDSQPSTWILGIAYRKARNAFR
                     SAARARARENLSPPDFPLTTEAPAHAEELRDWLVHALSELPVEQRLAVELCYELGHSC
                     EEIATIMGCPVNTVKTRLFHARAKLQKRLPELSGAVRGNAAESEAS"
     gene            904..1614
                     /locus_tag="VM713_08800"
     CDS             904..1614
                     /locus_tag="VM713_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HVH84400.1"
                     /translation="MSASPAFTHEESWMLLPWLANGRLGGAERALLEQHLRGCAECAR
                     EVELQRLWCSTLTEPERVTYAPGPSFRKLMERIDAEPPLRAGPAGIPLVRAVRARTAW
                     RPPGLAWAASFVLCVGLGTFALTAYRWSQPLYVTTTAPAAAAPGVLHIAFERSLTMGE
                     AERLLISAGARVVEGPDASGVFGIAPVAAAAAPGEGASELRSLADRLRRDSHVRWIEP
                     LAGSEQSDRAPAPLPRRP"
     gene            1625..>2942
                     /locus_tag="VM713_08805"
     CDS             1625..>2942
                     /locus_tag="VM713_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVH84401.1"
                     /translation="MRARARLLGLVLACAAAATDVRAVEPAAGPAAAVATPSALPRIV
                     VAFPNEPRSAPGPAGSTGRRYTGDGYRVSQSAEQLAARVAAAYSLREVASWPIRELAM
                     HCVVYEITSGRPAAAVLAALAHDPRVALAQPLQEFHTLTGPAPDPPPYNDPLYDLQTN
                     LPALGIPDAHRRAQGAGVRVALIDTAVDTHHPDLAGRIVRSRSFTGTNRGAGGSQRHG
                     TAMAGIIAAVANNNIGIVGIAPRAELEVFEACWQLEPDGDAAACNTFTLAKALAAALE
                     SGVPIVNLSVAGPADPLLAALVATGLRRGVVFVGAVDAGGGFPAGVPGVIGAAGSESV
                     VPPSALAAPARHMLTLRPGAQYDFGSGSSVAAAEITGVIALLMSATPARLSSDALLSL
                     LRPPQTAVTVSASDVTAVNAAAALARLDLERRRICSPSRSGPATAGC"
BASE COUNT          395 a         1095 c         1031 g          421 t
ORIGIN      
        1 gggttactac tatgccagcc accccgatca ccacccgccc cgggcggtcg attatgtcta
       61 tggcaacgcc ggcttcgctc tggagtggcg gcgctggcgc atcgacgtgg gctatttcgc
      121 ggcccagatg caggctgcgg agctcttccc ttacccgatc gccaagcgca tcgccggcac
      181 gatcagctgg cagttctgag ggcgcgcgcg cccagcccgt cgcagcggcg cttgaacccg
      241 ggcgctcggc gtggagactt accactaaag ggcgtgtcga ctgaacttga gcggctataa
      301 cgacatggtg gaggtgaccc cgcgcggcgg cagtgctgcg gcgcgcgagg ccgagctcat
      361 caagcgggtg gcgcgccacg accggcgcgc attcgaggag ctctacaacc tctaccatcg
      421 gcgcctggcg cgcttcctca cgcggctcac gcgccgcttc gacctcgccg aggagatcat
      481 caacgacacc ttcctggtcg tgtggcgcag cgccggcggt tttcgcggcg actcgcagcc
      541 ctcgacctgg atactcggga tcgcctaccg caaagcgcgc aacgcgttcc gcagcgccgc
      601 acgggcccgt gcccgggaga acctgagccc gcccgacttc ccacttacga ccgaggcacc
      661 ggcacatgcc gaggagctgc gcgactggct cgtgcacgcc ctgagcgaac tgccggtcga
      721 gcagcgcctg gccgtcgagc tgtgctacga gctcgggcat tcgtgcgagg agattgcgac
      781 gatcatggga tgtccggtga acacggttaa gacccgcctg tttcacgcgc gtgcgaagct
      841 gcagaaacgt ctgcccgagc tgtccggcgc agtgcgcggc aacgccgcgg agagcgaggc
      901 atcatgagtg caagcccggc tttcacccac gaagagtcct ggatgctgct gccgtggctc
      961 gccaacggcc ggctcggcgg cgccgagcgg gcccttctcg agcagcacct gcgcggctgc
     1021 gccgagtgcg cgcgcgaggt ggagctgcag cgcctctggt gcagcacgct gacggagccc
     1081 gagcgcgtca cctacgcccc cggcccctcg ttccgcaagc tgatggagcg catcgacgcg
     1141 gagccaccgc tgcgggccgg cccggcgggc atcccgctcg tgcgcgcggt gcgcgcgcgc
     1201 acggcgtggc ggccgcccgg tctcgcgtgg gcggcgagtt tcgtgttgtg cgtggggctc
     1261 ggcaccttcg ccctcacggc gtatcgctgg tcgcagcccc tgtacgtcac gaccaccgca
     1321 cccgccgccg ccgcgcccgg cgtgctgcac atcgcctttg agcgctcgct tacgatgggc
     1381 gaggccgagc ggctgctcat ctccgcgggc gcacgtgtcg tcgagggacc ggacgcgagc
     1441 ggagtcttcg ggatcgcgcc ggtcgccgcg gcggccgcgc ccggcgaggg cgcgagcgag
     1501 ctgcgctcgc tcgcggaccg tctgcgccgc gactcccatg tgcgctggat cgagccgctt
     1561 gccggcagtg agcagtccga ccgcgcaccc gcgccgctgc cgcggagacc ctgagttgtc
     1621 gcgcatgcgc gcccgcgcca ggctgcttgg gctcgtgctg gcttgcgcgg cggccgcaac
     1681 cgacgtacgc gcagtcgagc ccgccgcggg cccggcggcc gccgtggcga ccccgagcgc
     1741 cctgccgcga atcgtggtcg ccttccccaa cgagccgcgc agcgccccgg gaccggccgg
     1801 ctccaccggg cggcgctaca ccggggacgg ctaccgcgtg tcacagagcg ccgagcagct
     1861 tgccgcgcgc gtggcagccg catactcgct gcgcgaggtg gctagctggc cgatccgcga
     1921 gctcgccatg cattgcgtcg tgtacgagat caccagcggc cggccggccg ctgcggtgct
     1981 cgcggcgctc gcgcacgatc cgcgggtggc actcgcgcag ccgctgcagg agtttcatac
     2041 actgaccggg ccggcgccgg acccgccccc ttacaacgat ccgctctacg acctgcagac
     2101 gaacctgccg gcgctcggca ttcccgacgc gcaccgccgc gcccagggag ccggagtgcg
     2161 ggtcgcgctc atcgataccg cagtcgacac ccaccatccg gacctcgccg gccgcatcgt
     2221 acgcagccgc tcctttaccg gaacgaatcg cggcgcgggc ggctcgcagc gtcacggcac
     2281 ggccatggcg ggcatcatcg cggcggttgc caacaacaac atcggcatcg tcggcatcgc
     2341 gccacgcgcc gagctcgagg tgttcgaggc ttgctggcag ctcgagcccg acggcgacgc
     2401 cgcggcttgc aataccttca cgctcgccaa agcgctggcc gcggcgctcg agtccggcgt
     2461 gccgatcgtg aatctgagtg tggcgggccc ggccgacccg ctgctcgcgg cactggtcgc
     2521 gacaggactc aggcgcggtg tggtgttcgt cggggccgtg gatgcaggcg gcggtttccc
     2581 cgcgggtgtc cccggggtca taggggccgc gggcagcgag agtgtcgtcc ccccaagcgc
     2641 cctcgctgct ccggcccggc acatgctgac gctgcggccg ggtgcgcagt acgacttcgg
     2701 gtccgggagc tccgtcgccg cagccgagat caccggcgtg atcgcgctgc tcatgtcggc
     2761 gacgcccgcg cgcctcagca gcgatgccct gctgtccctc ctcaggccgc cccagaccgc
     2821 ggtgacggtc agcgccagcg acgtaacggc ggtcaatgcc gccgccgcgc tcgcgcgcct
     2881 cgatctggag cggcgccgca tctgcagccc ctcccgcagc gggcccgcga cggcgggctg
     2941 cg
//
LOCUS       DATLKL010000652         1523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_210884, whole genome shotgun sequence.
ACCESSION   DATLKL010000652 DATLKL010000000
VERSION     DATLKL010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210884"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..>1523)
                     /locus_tag="VM713_08810"
     CDS             complement(278..>1523)
                     /locus_tag="VM713_08810"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVH84402.1"
                     /translation="SRARQVPDLVLLDIWMPDVDGITLLREWSTGAADGCPVVMMSGH
                     GTVETAVEATRLGAFDFVEKPLSLAKLLRTVERALDAGRRHRQSGKLLGAALTAPIGK
                     SRTMQQLRAELQQMAANPSSLLLIGEPGSGREAFARYVHEHGARAAAPFITLIASTLR
                     EEDAEGRLFGREEPGKAREEGLLEQAGNGTLFLHEVEDLPVGVQRLLYGVLESGQFMR
                     LGGSEPVPLRARLISSAQPGIEQRAGSEALRRDLLAHLNVLIMRVPPLREYAEDVPDL
                     LRYHVDRVVDTEGLTFRRFSVAAQNRLRNYPWPDNVRELRHLVHRLLIHGGSEEIRLE
                     EIERELAVQTPPDEPLVKQDLLALPLREAREQFERAYLQQQLLLCNGKVGQLAKRVGM
                     ERTHLYRKLRSLGVDFRNISED"
BASE COUNT          233 a          530 c          524 g          236 t
ORIGIN      
        1 gaccagcaag ttcgtgcgca aggaaggcga tgtcatccgc cacaacatgg gctggacgtt
       61 catcctgctc gcgtacctga tcatcatcgc gatcgcgttc tatctcgtgc ggccggatgt
      121 gatggccctc ggcgcgggct gacgccgcgt agacgcgagc actgcagagg actttcgggg
      181 gcgtgcgcgg caggacgccg cgcccgcagc ctccatggat ggattcacgg cgtccccgaa
      241 acgccttgtc tggcgatcgc ggcggcgcgc atgagtgtca gtcttcgctg atattcctga
      301 agtcgacccc gagtgagcgg agcttccgat acaggtgcgt gcgctccatc cccacccgct
      361 tggcgagctg ccccaccttg ccgttgcaga gcagcagctg ctgctgaaga taggcacgct
      421 cgaactgctc gcgcgcctcc cgcagtggca gcgccagcag atcctgcttc accagcggct
      481 catcgggcgg ggtctgcacc gccagctcgc gctcgatttc ctcaaggcgg atctcctcgc
      541 tcccgccgtg gatcagcaac cgatgcacca ggtggcggag ctcgcgcacg ttgtccggcc
      601 aggggtagtt gcgcaggcga ttctgcgcag cgacgctgaa acgccggaag gtgagccctt
      661 cggtgtcgac cacccggtca acgtggtagc gcagcaggtc cggaacgtcc tcggcgtatt
      721 cgcgcaacgg cggcacgcgc atgatgagca cgttgaggtg tgcgaggagg tcgcggcgca
      781 gcgcctcgct cccggcgcgc tgctcaatgc cgggctgggc cgaggagatg aggcgggcgc
      841 gcaacggcac cggctcgctg ccgccgaggc gcatgaactg tccggactcg agcacgccgt
      901 acaggaggcg ctgcaccccg acaggcagat cttcgacctc gtggaggaag agcgtcccgt
      961 tgccggcctg ctcgagcagg ccctcctcgc gcgctttgcc cggctcctcg cgcccgaaga
     1021 ggcgcccctc cgcgtcctcc tcgcgcagcg tgctcgcgat gagcgtgatg aacggcgccg
     1081 ccgcgcgcgc accgtgctcg tgcacgtagc gcgcgaaggc ctcgcgtccc gagcccggct
     1141 cgccgatcaa cagcagcgag gaggggttgg ccgccatctg ctgcagctcg gcgcgcagct
     1201 gctgcatggt gcggctcttg ccgatcggcg ccgtgagcgc agccccgagg agcttgcccg
     1261 actggcgatg gcgccgcccg gcatcgagcg cgcgctcgac ggtacgcagc agcttggcga
     1321 gcgacagcgg cttctccacg aagtcgaagg ccccgaggcg cgtggcctcg actgcggtct
     1381 cgacggtgcc gtgtcccgac atcatgacga ccggacagcc gtcggcggcg ccggtggacc
     1441 actcgcgcag cagcgtgatg ccgtccacgt ccggcatcca gatgtcgagc agcacgaggt
     1501 ccggaacctg acgcgcgcgc gag
//
LOCUS       DATLKL010000653         2088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_98388, whole genome shotgun sequence.
ACCESSION   DATLKL010000653 DATLKL010000000
VERSION     DATLKL010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98388"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VM713_08815"
     CDS             <1..975
                     /locus_tag="VM713_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVH84403.1"
                     /translation="IFVGDTRVTRRQLLRYSLLGMAAFTTADALARASSPKPPPAERA
                     GTVKIGGDLTVNRLGFGAMRLAGGEHVWGEPQDGAAARRVLRRAVDLGVDFIDTADAY
                     GPDINERLICEALHPYPHGLVIATKGGIVRPSAPEWVPNGRPEHLREACEGSLKRLKL
                     ERIDLYQLHCVDDRVPLEESIGALADLQKEGKIRHIGVSNFKLDEVIRARGVAHIVSV
                     QNRYNFAQRKSADVLDLCAREGLVFIPWAPIAHGSSQTLEQSTGSKDLEALASAHGAS
                     VVQIAIAWLLAKSPAMLPIPGTSSVAHLEENVAAARIHLSEAELERLG"
     gene            1120..1590
                     /locus_tag="VM713_08820"
     CDS             1120..1590
                     /locus_tag="VM713_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVH84404.1"
                     /translation="MTIDFVLNRRRASFAGDPGMPLLWYLRDAARLTGTRFGCGIGYC
                     GACTVHVDGSARRACMTPMSSIAGHEVLTIEGLAGASTELHPVQEAWIEEDVPQCGYC
                     QAGQIMAAVDLLKRKPNPTDEDIDGITNLCRCGTDPRIRKAIRRAAARLREEGK"
     gene            1587..>2088
                     /locus_tag="VM713_08825"
     CDS             1587..>2088
                     /locus_tag="VM713_08825"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVH84405.1"
                     /translation="MSAPLRPFSPSRRQFIRISASAAGGLVLSWCLPECDAAEETPGS
                     RVSAIPPSVPQGEWVGAFVRIEPDGRIVIGARGCEIGQGVRTSLPMLIAEELDVPWSM
                     VAVEQLPYGVRYGPQGAGGSTSIPDGWQDLRQAGARARWLLVQAAAARWSTSTTELRT
                     QDGQVLH"
BASE COUNT          371 a          659 c          715 g          343 t
ORIGIN      
        1 atcttcgtag gtgatacccg agtgacccgc aggcaactgc tgagatacag tctcctcggc
       61 atggctgcat tcaccaccgc tgacgcgctc gcgcgggcgt catcgccgaa gccgcccccc
      121 gcggaacgtg cgggcaccgt aaagatcggc ggcgacctca cggtgaatcg gctcggcttc
      181 ggcgccatgc gcctcgctgg aggcgagcac gtttggggag aaccgcagga cggcgccgcc
      241 gcacggcggg tgttacgccg ggcggttgac ctcggcgtcg actttatcga taccgccgat
      301 gcctacgggc cggatatcaa cgagcggctg atctgcgagg cgctccatcc ttacccgcac
      361 ggactcgtga tcgccacgaa gggcggcatc gtgcgcccca gcgctccgga gtgggttccg
      421 aatggccgcc cagagcatct gcgggaggcc tgcgaaggaa gcctcaaacg cctcaagctc
      481 gagcgcatcg acctctacca attgcattgc gtcgacgacc gggtaccgct cgaggagtcg
      541 atcggggcgc ttgcggacct ccagaaggaa ggcaagatcc gccatatcgg ggtctcgaac
      601 ttcaagctcg atgaggtcat cagggcacgg ggggtcgcgc acatcgtatc ggtgcagaac
      661 cgctataact tcgcacagcg caaatccgcc gacgtgctgg acttgtgcgc gcgcgaaggc
      721 ctcgtgttca ttccgtgggc gcccatcgcg cacggttcct cgcagacact cgagcagagc
      781 acgggcagca aagatctcga ggcgctcgcg agcgcgcacg gcgcgagcgt cgtgcaaata
      841 gcgatcgcct ggctgctggc aaagtcaccg gcgatgctgc cgatccccgg tacgtcatcg
      901 gtcgctcatc tggaggaaaa cgtagcggcg gcgcgcatcc acttaagtga agccgagctc
      961 gaacgcctgg gataaagaga gtagcaacag gtcggatgag tgatttggcc gcaattgagt
     1021 ctcggatggc cgcatccgag gcggacggcg gcggtctgcg gcggtaaatt cgcggcggcg
     1081 cgagtatcgg ccgcgccctg cgcatgcacg gagaccattt tgacgatcga ctttgtgctg
     1141 aatcgacggc gcgcgagttt cgcgggcgac ccaggcatgc cgctgctttg gtaccttcgg
     1201 gatgcggcgc gcctcaccgg caccagattt ggctgtggta tcggttattg tggcgcgtgc
     1261 acggtgcatg tcgatggcag cgcgcgccgt gcgtgcatga cgccaatgtc ctcgatcgcc
     1321 gggcacgagg tgctgacgat tgaaggtctg gcaggcgcca gtaccgagct gcatcccgtc
     1381 caggaggcct ggatcgagga agatgtgccc cagtgcgggt actgtcaggc agggcagatc
     1441 atggccgcgg ttgacctgtt gaagcgcaag cccaacccga ccgatgaaga catcgacggc
     1501 ataaccaacc tgtgccgctg cggcacggat ccacgaatac gaaaggccat ccgccgcgcg
     1561 gccgcccgtc ttcgcgagga aggcaagtga gcgcgccgct gcgcccattc tcgccatcgc
     1621 gacgccagtt catacgaatc tcggcgagcg ccgccggcgg cctggtgctg tcgtggtgcc
     1681 tgccggagtg cgacgccgcc gaagaaacac ccgggagcag ggtctcggcc atcccgcctt
     1741 cggtcccgca aggcgaatgg gtcggcgcct tcgtgcgcat cgagcccgac gggcgcatcg
     1801 taatcggtgc gcgcggctgc gagatcggtc agggtgtccg cacctctctt ccgatgctga
     1861 ttgcggagga gctggacgtg ccctggtcga tggtcgcggt cgagcagctg ccctacggag
     1921 ttcggtatgg accccagggc gcggggggca gcaccagcat ccccgacggg tggcaagacc
     1981 ttcggcaagc gggagcgcgt gcccggtggc tgctcgtgca ggcagccgcc gcgcgctgga
     2041 gtacatcgac gaccgagttg cggacacagg acggccaggt cttgcatc
//
LOCUS       DATLKL010000654         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_165896, whole genome shotgun sequence.
ACCESSION   DATLKL010000654 DATLKL010000000
VERSION     DATLKL010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165896"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1443)
                     /locus_tag="VM713_08830"
     CDS             complement(82..1443)
                     /locus_tag="VM713_08830"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HVH84406.1"
                     /translation="MRSTMLGGAFAAAALAATAAQADFNAALADYKAGHYDIARSQFS
                     AMAELGDCSSQFNLGVMVLQGQGGAKDVGTGIGWLEAAAANGCQELVGGRVAALKGSL
                     SAPEERTAADIMARYGHEALHAQGIVDPELDCRARLAATVLEAPTPEYPRSGNRRNGL
                     VIGELTIGVDGHARDPEILLAAPDDAFAAPAVEAWLHARFTPATRDGAPVESRQQVRL
                     PFAIAGGEVLWSSARYKEARAAADAGDPAAEYLVGLAATADPALGVGAARGTQLVINA
                     ARDGNREAQYWLGEQLRSVAACHPKTSGAAWLKHAAAGGDAGAQLQLAADLLSASPSD
                     AQLNEARTLLEHAAAADSYYVRKHVVGLLAASPFVTLRDPATARQLALKLSAGPIQCD
                     PQMFEALAAAAAAAGDFASAVSQQETAIRKARDLAWKTRAMEARLASYKGGKAWQGDV
                     FAL"
     gene            complement(1473..2756)
                     /locus_tag="VM713_08835"
     CDS             complement(1473..2756)
                     /locus_tag="VM713_08835"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HVH84407.1"
                     /translation="MLVGQAARRVAIVGGMRIPFARSYTAYATASNQDMLTAAFRAVV
                     ERFKLQGERLGDVAAGAVIKHSKDYSLTRESVLGVGLDPQTPGLDLQRACGTSLEAAI
                     AIGNKIALGQIDAGIAGGTDSISDAPIVYPRSYQQLLLASYRGRSAWQRFKPFLGLRP
                     KHFRPTLPAVVEPRTGLSMGQSCELMAKRWQITRAAQDQLAYDSHVKAAAAWREGFYD
                     DLVVPFAGLKTDNNVRADTSVEKLAKLRPSFAADGTLTAGNSTPLTDGASAVLLATPE
                     WAAKRSLPVQAYLAYGKVWAVDYVSGKEGLLMAPAYAVPAMLRDANLKLQDFDYYEIH
                     EAFAAQVLCTLKAWETPEYCRDVLGLPGPLGSIDRARLNVKGGSVALGHPFAATGTRI
                     LATLAKLLAGNASARRGLMSVCTAGGMGVTAIVER"
BASE COUNT          409 a         1020 c          995 g          444 t
ORIGIN      
        1 gagcgcggag cagcgcgcac tgctcgactt cctgctcatt tgacgcagcc caagctctga
       61 cgtgtcaggg cttcggggct gctacaaggc aaacacgtcg ccctgccagg ccttgccgcc
      121 cttgtaggag gcgagccgcg cctccatggc gcgcgtcttc cacgcgaggt cgcgcgcttt
      181 gcggatcgcg gtctcctgct gcgagactgc gctggcgaaa tcgccagctg cggccgccgc
      241 cgcggccagc gcttcgaaca tctgcggatc gcactggatc gggccggcgg acagcttgag
      301 ggccagctgc cgcgccgtcg cgggatcacg cagcgtcacg aacggcgacg ccgcgagcag
      361 cccgacgacg tgctttctca cgtagtagct atcggcggca gcggcgtgct cgagcagcgt
      421 gcgcgcttcg ttaagctgcg cgtccgaggg actggcgctc agcagatccg cagcgagctg
      481 cagctgcgcg ccggcatcgc cgcccgccgc ggcgtgtttg agccacgccg caccactggt
      541 cttcggatgg caggcggcca cggaccgcag ctgctcgccg agccagtatt gggcctcacg
      601 gttgccgtcg cgcgccgcat tgatcaccag ctgtgtcccg cgtgcggcac ccaccccgag
      661 cgccgggtcg gccgtcgctg cgaggcccac caggtactcg gcggccggat cgccggcatc
      721 cgccgccgcc cgcgcctcct tgtaccgcgc gctgctccac aacacctcgc cgcctgcgat
      781 ggcaaacggc aaccgcacct gctgccgcga ttccaccggc gcgccgtcgc gcgtcgctgg
      841 cgtgaagcgc gcgtgcagcc acgcctcgac cgctggcgcg gcgaacgcat cgtcgggcgc
      901 cgcgagcaga atctccgggt cgcgcgcatg gccgtcgacg ccgatagtga gctcgccgat
      961 cacgaggccg ttgcggcggt tgccgctacg cggatactcg ggcgtcggcg cctccagcac
     1021 cgtcgccgcg aggcgcgcgc ggcagtcgag ctcggggtcg acgattccct gcgcgtgcag
     1081 cgcctcgtga ccgtagcgcg ccatgatgtc cgcggccgtg cgctcctccg gcgcactcag
     1141 cgaacccttg agcgccgcga cccggccgcc gaccagctcc tggcagccgt tggccgccgc
     1201 cgcctcgagc cagccgatgc ccgttccaac atccttcgcg ccgccctgac cctgcagcac
     1261 catcacaccg aggttgaact gcgaggagca gtcgccgagc tcggccatcg cgctgaactg
     1321 gctccgggcg atgtcatagt ggccagcctt gtagtcggcg agcgccgcgt tgaaatcggc
     1381 ctgggcagcc gtggcggcga gtgccgcggc tgcgaatgcg ccccctagca ttgtagatct
     1441 catggtccgc tcccccggcg tctcgtcggt gatcagcgct cgacgatcgc ggtgactccc
     1501 atcccgccgg cggtgcacac cgacatcagc ccgcgcctgg cggaagcgtt gccggcgaga
     1561 agcttggcaa gcgtggccag aatgcgcgtg ccggtcgcag cgaaggggtg gccgagcgcg
     1621 acgctgccgc ccttcacatt cagcctggcg cgatcgatgg accccaaggg ccctggaagg
     1681 ccgagcacgt cgcggcagta ctccggcgtc tcccacgcct tgagcgtgca cagcacctgc
     1741 gcagcgaacg cctcgtgaat ctcgtaatag tcgaagtcct gaagcttgag gttcgcatcg
     1801 cgcagcatgg cgggcacggc gtaggcgggc gccatcagca ggccctcctt gccgctcacg
     1861 tagtcgaccg cccagacctt gccgtaggcg agataggcct gcaccgggag gctgcgcttc
     1921 gcggcccact cgggtgtcgc gagcagcacg gcgctcgcgc cgtcggtgag cggcgtcgag
     1981 ttgccggcgg tgagcgtgcc atcggcggcg aagctcgggc gcagtttcgc gagcttctcg
     2041 acgctggtat cggcgcgcac gttgttgtcg gtcttgaggc ccgcgaacgg gactaccagg
     2101 tcgtcgtaga acccctcgcg ccaggcggcc gccgccttga cgtggctgtc gtaggcaagc
     2161 tggtcctgcg cggcgcgcgt gatctgccag cgcttcgcca tgagctcaca gctctggccc
     2221 atggagagtc cggtgcgcgg ctcgaccacc gccggcagcg tcgggcggaa atgcttcggc
     2281 cgcaggccga ggaacggctt gaagcgctgc caggcgctgc ggccacggta gctcgcgagc
     2341 agcagctgct ggtaggagcg gggatagacg atcggcgcgt cgctgatcga gtcggtgccg
     2401 ccggcgatgc cggcgtcgat ctggccgagg gcgatcttgt tgccgatggc gatcgccgcc
     2461 tcgaggctcg tgccgcaggc gcgctgcagg tcgagtcccg gcgtctgcgg gtcgagcccg
     2521 acgccgagca cgctctcgcg cgtcaggctg tagtccttcg agtgcttgat gaccgcgccc
     2581 gcggccacgt cgccgagccg ctcgccctgc agcttgaagc gctcgaccac ggcgcgaaat
     2641 gcggccgtga gcatgtcctg attgctggcc gtggcgtagg ccgtgtagga gcgggcgaac
     2701 ggaatgcgca tgccaccgac gatggcgacg cgcctggctg cttgaccgac gagcatgaca
     2761 cctccccgga gcgcggcagg ttagaatgcc atggtccaga aggcgttgcc atcggatccc
     2821 catgggcgag ctcatcacca tcatggcccg cgacggtcac gagtttca
//
LOCUS       DATLKL010000655         1618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_98392, whole genome shotgun sequence.
ACCESSION   DATLKL010000655 DATLKL010000000
VERSION     DATLKL010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98392"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1483
                     /locus_tag="VM713_08840"
     CDS             170..1483
                     /locus_tag="VM713_08840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH84408.1"
                     /translation="MSFWQIWNMCFGFLGLQFGFALQNANASRIFQSLGASSEQLPLL
                     WFAAPCSGLLVQPLVGYFSDRTWTRLGRRRPYFLVGAALAATALVLMPRAPTLWAAAA
                     LLWLLDGAFNTSMEPFRAFVGDQLGPGQRPAGYSMQSFFIGAGAVVASFLPWLLERGG
                     VGNSTAAVHGSAIPDTVRYAFEIGAAVLVLAIGWTTLTSREYPPQELLAFDDALPAGS
                     DRAVGPGARGGLLATVVGDLANMPLAMWRLAPVQFFSWLALFSMWIYTTPAVAQVHFG
                     ATDPHSAAYNQGANWVGVLFGAYNGFAAVAAAIIPPMVRRLGLHLTHLVNVWLGGAAL
                     LSFLVIRDPDWLVVSMIGMGFAWASVLSLPYALLSDSVPAQKMGLYMGVFNIFIVIPQ
                     MVAASVLGFLLRVLFGGAPIYALVLGGVSFIVAGLLALRVPQPES"
BASE COUNT          197 a          539 c          539 g          343 t
ORIGIN      
        1 cgcggccgcg actcctgcga ccgccgccag gcatgatgcg acatacaaca gtacagctgc
       61 gcgtgcgagc gccgttgacc gactcgagac tgtgtgcata aatcgcgccg ccttggcccg
      121 gccggagctg cccgcatact acgcgcaaat gccgcacccg aaacccatat tgtccttctg
      181 gcagatctgg aacatgtgct tcggattcct cggcctgcag ttcggctttg ccctgcagaa
      241 cgctaacgcg agccgtatct tccagtccct cggtgcgagc agcgagcagt tgccgctgct
      301 gtggtttgcc gcaccctgca gcgggctgct ggtgcagccg ctggtaggtt acttctccga
      361 ccggacctgg acgcgccttg ggcggcgccg gccgtacttt ctggtgggcg cagcgctcgc
      421 cgccacggcg ctcgtactca tgccgcgcgc gccgacgctg tgggccgcgg cggcgctcct
      481 gtggctcctt gatggtgctt tcaacacatc catggagccc ttccgggctt tcgtgggcga
      541 tcagctgggc ccgggtcagc gcccggcggg ctattcgatg cagagcttct tcatcggcgc
      601 cggggctgtt gtggcgagct ttctgccctg gctgctggag cgcggcggtg tcggcaactc
      661 caccgctgcg gttcatggga gtgccatccc cgacaccgta cgctatgcct tcgagatcgg
      721 cgctgcggtg ctggtacttg cgatcggctg gaccacgctc acctcacgcg agtatccgcc
      781 gcaggagctg ctcgccttcg acgacgcctt gcccgccggc tccgaccgcg cagtcggtcc
      841 gggcgcgcgc ggcggcctgc tcgcaaccgt tgtgggtgat ctcgcgaaca tgccgctcgc
      901 gatgtggcgg ctcgctccgg tgcagttctt ctcgtggctg gcgctcttct ccatgtggat
      961 ctacacgact cctgcggtgg cgcaggtgca cttcggcgcc accgatccgc attccgccgc
     1021 ctacaaccag ggcgccaact gggtcggcgt gctgtttggg gcctacaacg gctttgcggc
     1081 tgtcgctgcc gcgatcattc cgccgatggt gcgccgcctg gggctgcacc tcacgcatct
     1141 ggtgaatgtg tggctgggcg gtgcggcgct gctgtcgttc ctggtgatcc gtgacccgga
     1201 ctggcttgtg gtcagcatga tagggatggg cttcgcctgg gcgtcggtgc tgtcactccc
     1261 ctatgcactg ctctccgaca gcgtgcccgc gcagaagatg ggcctgtaca tgggcgtctt
     1321 caatatcttc atcgtgattc cgcagatggt ggccgccagc gtgcttggct ttctgctccg
     1381 ggtcctgttc ggtggtgcgc cgatctacgc gctggtgctc ggcggtgtga gttttatcgt
     1441 cgcgggcctg ctcgctttgc gcgtcccgca accggagagt tgaccatgtc aagtccctcg
     1501 cgctcccttg gaacggtgct ggtcgccgcc gtgctgcttg tgctgctggc ggcgggtgcg
     1561 caagccgcgc agccgcagac aaccgtgacg ttcgcgccgg ccgtggcgct gaggatgg
//
LOCUS       DATLKL010000656         1854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_52947, whole genome shotgun sequence.
ACCESSION   DATLKL010000656 DATLKL010000000
VERSION     DATLKL010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52947"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /gene="aceE"
                     /locus_tag="VM713_08845"
     CDS             complement(<1..1257)
                     /gene="aceE"
                     /locus_tag="VM713_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HVH84409.1"
                     /translation="MTEPPKLEDLDPVETREWLESIDSVLKTHGPERAHFLLERLIDY
                     TRRSGAYLPFKPNTAYVNSIPSGREPEYPGNRALERRIEAYIRWNALAMVLLANRKSS
                     EYGGHLASYASSATLYEVGFNHFWRAPSEQHPGDLVFIQGHSSPGIYARAFLEGRLTE
                     GQLRHFRQEAGGRGLGLSSYPHPWLMPDFWQFPTVSMGLGPMMAMFQARFTRYLEHRG
                     LVKPSDQKVWAFLGDGEMDEPESMGALTMPVREGLDNLIFVINCNLQRLDGPVRGNGK
                     IIQELEAAFLGAGWNVIKVLWGSRWDPLLARDYKGILRRVMEETVDGEYQNFKAKGGA
                     FTREHFFGKDPELKAMVANMSDDEIWRLNRGGHDPLKVYAAYAAAVEHKTQPTVILAK
                     TVKGFGLGKGGEGQMIAHQQKKLSEED"
     gene            1459..>1854
                     /locus_tag="VM713_08850"
     CDS             1459..>1854
                     /locus_tag="VM713_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84410.1"
                     /translation="MTSLTMRRPDDWHVHLRDGAALAAVLPFTATRFARAIVMPNLQP
                     AVTTTALALDYRRRILAALPPGVAFEPLMTLYLTDDTPLAEVERAKRSGCIFGCKLYP
                     AGATTHSDSGVTDVARIAAVLERMAELGLV"
BASE COUNT          287 a          632 c          620 g          315 t
ORIGIN      
        1 gtcctcctcg gagagcttct tctgctggtg ggcaatcatc tggccttcgc cgcccttgcc
       61 gaggccgaat cccttgacgg tcttggcgag aatgacggtt ggctgggtct tgtgctcgac
      121 ggcggcggcg taggccgcgt acaccttgag cggatcgtgg ccgccgcggt tgagccgcca
      181 gatctcatcg tccgacatgt tggcgaccat cgccttgagc tcgggatcct tgccgaagaa
      241 atgctcgcgg gtgaaggcgc cgcccttggc cttgaagttc tggtattcgc cgtcgacggt
      301 ctcctccatc acgcggcgca ggatgccctt gtagtcgcgg gccagcagcg gatcccagcg
      361 cgagccccac agcaccttga tgacgttcca gccggcaccg agaaaggcgg cctcgagctc
      421 ctggatgatc ttgccgttgc cgcgcaccgg accgtcgagc cgctgcaggt tgcagttgat
      481 gacgaagatc aggttgtcga gcccctcgcg caccggcatg gtgagcgcgc ccatcgactc
      541 gggctcgtcc atctcgccgt cgccgagaaa cgcccagacc ttctggtcgg agggtttcac
      601 caggccgcgg tgctcgagat agcgggtgaa gcgcgcctgg aacatcgcca tcatcggacc
      661 caggcccatc gacaccgtgg gaaactgcca gaagtccggc atcagccacg gatgcggata
      721 ggacgagagc ccgaggccgc gcccgccggc ttcctggcgg aagtggcgca actgcccctc
      781 cgtcagacgc ccctcgagaa aggcgcgcgc gtagatcccc ggtgaggaat gcccctggat
      841 aaacaccaag tcaccgggat gctgctcgga gggcgcacgc cagaagtgat tgaacccgac
      901 ctcgtaaagc gtcgccgacg aggcgtagct cgccaggtgc ccgccgtact cggaggactt
      961 gcgattggcg agcagcacca tcgcgagcgc gttccagcgg atgtacgcct cgatgcgccg
     1021 ctcgagcgcg cggttgcccg ggtactccgg ctcacgcccg ctcgggatgc tgttgacgta
     1081 ggcggtgttg ggcttaaacg gcaggtaggc gccgctgcgg cgcgtgtagt cgatgagcct
     1141 ctcgagcagg aagtgcgcac gctcgggtcc gtgggtcttc agcaccgagt cgatcgattc
     1201 gagccactcc cgcgtctcga cgggatcgag gtcttccagc ttgggcggtt cggtcatgtg
     1261 gtctgcctgg ttgcgctcgg cgcgcgcagc taagccccgt ccgacggcgc gctcgcgggt
     1321 cttggtcgca ggcgtgttgt gccgcgtcgc gatagaatga gtaagcaatg atggctagcg
     1381 ccggcaggta aagcaagtga gcccgacggc ctccgccgcc cggcggcctg tggcgcgcgc
     1441 ggcaccggag cggcgcgcgt gacgagcctc accatgcgcc ggccggacga ctggcacgtg
     1501 cacctgcgcg acggggcagc gctcgcagcg gtgctgccat tcaccgccac gcgctttgcg
     1561 cgcgccatcg tcatgccgaa cttgcagccc gcggtgacta ctaccgcgct ggcgctcgat
     1621 taccgccgcc gcatcctcgc cgcattaccg cccggcgtcg ccttcgagcc gctcatgact
     1681 ctctatctca ccgacgacac gccgctcgcc gaggtcgagc gggccaagcg cagcggctgc
     1741 atcttcggct gcaagctcta tccggccggc gccaccaccc attccgacag cggcgtgacc
     1801 gacgtcgcgc gcatcgccgc ggtgctcgag cgcatggccg agctcgggct ggtc
//
LOCUS       DATLKL010000657         1510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_86908, whole genome shotgun sequence.
ACCESSION   DATLKL010000657 DATLKL010000000
VERSION     DATLKL010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86908"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VM713_08855"
     CDS             complement(<1..316)
                     /locus_tag="VM713_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVH84411.1"
                     /translation="MEFRIKALDLTSDVITCVVDAVDEADARRQLALRELKVISLQPV
                     RRLRRLLQSPHLQVAVFSQQLVSLLEAGLSLVEALEALAQKESNTATKGILDQILARL
                     YEG"
     gene            complement(326..748)
                     /gene="gspG"
                     /locus_tag="VM713_08860"
     CDS             complement(326..748)
                     /gene="gspG"
                     /locus_tag="VM713_08860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HVH84412.1"
                     /translation="MRRKLGRSQGFTLLELLVVMVIIGLLAGFVAPRYFAQVGKSRVK
                     AAHAQIDALDKALEQYRLDVGRLPTTEEGLAALNVAPQGATNWGGPYLKKDVPLDPWG
                     HAYVYAQPGTHQNDFDLISYGRDGQPGGTGEDADITNY"
     gene            complement(899..>1510)
                     /locus_tag="VM713_08865"
     CDS             complement(899..>1510)
                     /locus_tag="VM713_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HVH84413.1"
                     /translation="LRAFSYVCFFSLCVQPYVARADIYSFVDEAGVTHFSNVPTDARY
                     RLMLASPPDPAQSQEKVGDGRWLKRSHAFDSLIDRAASEATVRPQLVRAIIVVESAFN
                     PKAVSRRGAAGLMQLLPKTARRYGAKNVFDPEQNITAGTRYLRDLMSRYGDNLELVLA
                     AYNAGEDAVERYGKQVPPFAETRQYVPAVLRIYRALLASHASS"
BASE COUNT          285 a          471 c          492 g          262 t
ORIGIN      
        1 gtccctcgta gagacgcgcg agaatctgat cgaggatgcc cttggtggct gtgttggatt
       61 ccttctgagc gagcgcctcg agcgcctcga ccagtgagag cccggcctcg aggagcgaca
      121 ccagctgctg gctgaagacc gcgacctgca ggtgcggcga ctgcagcaga cggcgcaacc
      181 ggcgcaccgg ttgcagcgag atgactttca gctcacggag cgcgagctgg cggcgcgcgt
      241 cggcctcgtc caccgcgtcg accacgcaag tgatgacgtc agaggtgagg tcgagtgcct
      301 tgatgcgaaa ctccacggca gcggctcaat agttggtgat gtccgcgtcc tcaccagtcc
      361 ctccgggctg accatcgcgg ccgtaggaga tgagatcgaa atcgttctgg tgggttcccg
      421 gctgcgcgta cacgtaagca tgaccccagg ggtcgagcgg tacgtccttc ttcagatacg
      481 gcccgcccca gttggttgct ccctgcggag cgacattgag cgccgcgagt ccctcctccg
      541 tcgtcggcag ccggccgaca tcgagccggt actgctcgag cgccttgtcg agcgcgtcga
      601 tctgcgcatg cgccgccttc acccgcgact tgccaacctg cgcgaagtag cgcggtgcaa
      661 caaagccggc gagcagcccg atgatcacca tgacgaccag gagctcgaga agcgtaaacc
      721 cctgcgagcg ccccagcttg cgtcgcactg ccagatccat gccgaccacc ccgatttgtt
      781 gtgtgagtaa ggtgttccta gcctccatcg tcgtgaaaga tccgggctcc cgacagtggg
      841 cgggcgctct agcggtgggc gggaaaatga gtaagcgcgg gtagggattt actgtaggtc
      901 agctcgaggc atgcgaggcg agcagagccc gatagatccg cagcaccgcc ggcacgtatt
      961 gacgggtctc cgcaaagggg ggcacctgct taccatagcg ctcgacggca tcctcgcccg
     1021 cgttgtaggc ggcaagaaca agctcaaggt tgtcaccata acgtgacatc aggtctcgta
     1081 aatagcgggt tccggcggtg atgttctgct caggatcgaa gacattcttg gctccgtagc
     1141 ggcgcgccgt cttcggcagc agctgcatga ggccagccgc accgcgccgc gacaccgcct
     1201 tggggttgaa tgcggactcg acgacgatga tggcgcgcac caactgcggg cgcaccgtcg
     1261 cctcgctcgc ggcgcgatca atcagggaat caaaggcatg tgagcgcttg agccagcgcc
     1321 cgtccccgac cttctcctgg ctctgcgccg ggtccggcgg tgaagccaac atcaggcgat
     1381 agcgggcgtc ggtcgggacg ttgctgaagt gcgtgacgcc cgcttcgtct acgaagctat
     1441 agatatccgc gcgcgcgaca taaggttgca cgcacaggct gaaaaagcag acgtaactga
     1501 atgcgcggag
//
LOCUS       DATLKL010000658         2494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_19142, whole genome shotgun sequence.
ACCESSION   DATLKL010000658 DATLKL010000000
VERSION     DATLKL010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19142"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VM713_08870"
     CDS             complement(<1..152)
                     /locus_tag="VM713_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HVH84414.1"
                     /translation="MVARSANLPRHIAITMDGNGRWAAARGVPRAAGHKAGLKPVRMC
                     VEECTRL"
     gene            complement(152..709)
                     /gene="frr"
                     /locus_tag="VM713_08875"
     CDS             complement(152..709)
                     /gene="frr"
                     /locus_tag="VM713_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018679060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HVH84415.1"
                     /translation="MLEELKKDAATRMAKCVQTFQADLKKLRTGRAHPSLVEHLKVDY
                     YGSEVPLQQVASIAVEEGRTLVISPWEKSVVQAIEKAIFKSDLGRTPMTAGTVIRIPM
                     PPLTEERRKEITKVLRHDAENARVAVRNVRRDVMNEIKHKMKDKHLSQDDERRTETEI
                     QKLTDKHVADIDAALAAKEKEVMQV"
     gene            complement(738..1463)
                     /gene="pyrH"
                     /locus_tag="VM713_08880"
     CDS             complement(738..1463)
                     /gene="pyrH"
                     /locus_tag="VM713_08880"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HVH84416.1"
                     /translation="MTQAKPRYTRILVKLSGEALLGESDYGIDPSVLKRIAGELQDIV
                     QMGVQVAVVIGGGNIFRGAGLARAGMDRVAADHMGMLATVMNSLALQDALESLGLHAR
                     VMSAIRINEVCEDYIRRRAVRHLEKGRIAVFAAGTGNPFFTADTAAALRAIEINADVL
                     LKATKVNGVFSDDPVRNSSAVRYARLTFDKVLTDKLNVMDATAIVMCRDNRLPLRVFN
                     LNNPGDLTRIVRGEDVGTAVTNE"
     gene            complement(1534..2412)
                     /gene="tsf"
                     /locus_tag="VM713_08885"
     CDS             complement(1534..2412)
                     /gene="tsf"
                     /locus_tag="VM713_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HVH84417.1"
                     /translation="MNITADTVKQLRERTGAGMMECKKALVETKGDLDAAAELMRKQG
                     LAKADKKAARVAAEGVIVIAKGSDARSAAMVEVNCETDFVARGPDFRAFAAAVAELAL
                     AARPADLAALLEARLASAESVEQRRRELVAKIGENISVRRFAVLTSPDQLGAYVHGTR
                     IGALVAVKGGEASLAYDLAMHVAASSPKYLSAQQVPAEVVAKEREILTSQAQGEGKPP
                     EIVAKMVEGRLRKALSEITLYGQAFVKDPEVTIEKLVKGAKAEVVAFERFEVGAGIEK
                     RADDFGAEVARLATGS"
BASE COUNT          335 a          898 c          798 g          463 t
ORIGIN      
        1 aggcgcgtgc actcctccac gcacatcctc acgggcttga gccccgcctt gtggccggcg
       61 gcgcgcggca cgccgcgcgc cgcggcccag cggccgttac cgtccatcgt aatggcgatg
      121 tgccgcggca gattggcgct cctcgcgacc atcagacctg catgacctcc ttctccttgg
      181 cggcgagcgc cgcgtcgatg tcggcgacgt gcttgtcggt gagcttctgg atctcggtct
      241 cggtgcgccg ctcgtcgtcc tgcgagaggt gcttgtcctt catcttgtgc ttgatctcgt
      301 tcatgacatc gcggcgcacg ttgcgcaccg cgacgcgcgc gttctcggca tcgtggcgca
      361 gcaccttggt gatctccttg cgccgctcct cggtgagcgg cggcatggga atgcggatca
      421 cggtgccggc ggtcatgggt gtgcggccga ggtccgactt gaagatcgcc ttctcgatag
      481 cctgcacgac gctcttctcc cagggtgaga tcaccagcgt ccgcccctcc tcgaccgcga
      541 tgctcgctac ctgctgcagc ggcacttcgc tcccgtagta gtcgaccttg agatgctcga
      601 cgaggctcgg gtgcgcgcgc ccggtccgca gcttcttcag gtccgcctgg aaggtctgga
      661 cgcacttggc catgcgcgtg gcggcgtcct tcttcagttc ttcgagcatg atgtctcctc
      721 gcggactgca gcgcggctca ctcgttggtg accgcggtgc cgacgtcctc gccgcgcacg
      781 atgcgggtga ggtcccccgg attgttgaga ttgaagacgc gcaaaggcaa gcggttgtca
      841 cggcacatga cgatggcggt cgcatccatt acgttcagct tgtcggtgag caccttgtcg
      901 aaggtgaggc gcgcatagcg taccgctgaa ctgttgcgta ctggatcgtc cgaaaagacg
      961 ccgttcacct tggtggcctt gagaagcacg tcggcgttga tctcgatggc gcgcagcgcc
     1021 gccgccgtgt cggccgtgaa gaacgggtta cccgtgccgg cggcgaacac cgcgatgcgg
     1081 cccttctcga ggtgacggac cgcgcgccgg cggatgtagt cctcgcatac ctcgttgatg
     1141 cggatcgcag acatcacccg cgcgtgcagt ccgaggctct cgagagcatc ctggagtgcg
     1201 agcgagttca tgacggtggc gagcatcccc atgtggtccg cggccacgcg gtccatgccg
     1261 gcgcgggcca gccccgcgcc gcggaagatg ttgccacccc cgatcaccac cgccacctgc
     1321 acccccatct gcacgatgtc ctgcagctcg ccggcgatgc gcttgagcac cgacggatcg
     1381 atgccgtagt cgctctcccc gagcagcgcc tcgcccgaga gcttcaccag gatccgcgtg
     1441 taacgcggct tcgcttgcgt catcttgcac ctccgccgat ggaaaaaacc ccgcgcgcgg
     1501 cggggttctc ttccctggct gcagcactcg tcatcagctt ccggtcgcca gccgtgccac
     1561 ctcggcgccg aaatcgtcgg ccctcttctc gatcccggcc ccgacctcga aacgctcgaa
     1621 cgcgaccacc tcggccttcg cgcccttcac gagcttctcg atcgtgacct ccgggtcctt
     1681 gacgaacgcc tgcccgtaga gggtgatctc gctcagggcc ttgcgcagac gcccctcgac
     1741 catcttggcg acgatctcgg gcggcttgcc ctcgccctgc gcctggctcg tgaggatctc
     1801 gcgctctttg gcgaccacct cggcgggcac ctgctgcgcg ctgaggtatt tcgggctgct
     1861 cgcggccacg tgcatcgcca ggtcataggc gaggctcgcc tcgccgccct tgacggcgac
     1921 cagcgcgccg atgcgcgtgc cgtgcacgta ggcgccgagc tgatcgggcg aggtgagcac
     1981 ggcaaagcgc cgcacgctga tgttctcgcc gatcttcgcg accagctcgc ggcggcgctg
     2041 ctcgacgctc tcagcggagg cgaggcgcgc ctcgaggagg gccgccagat ccgccggccg
     2101 cgcggcgagc gcgagctcgg ccaccgccgc ggcgaatgcg cgaaagtccg gtccccgcgc
     2161 cacgaagtcc gtctcgcaat tcacctcgac catcgcagcg ctgcgtgcgt cgctcccctt
     2221 ggcgatcacg atcacgccct cggccgcgac gcgcgccgcc ttcttgtcgg ccttggcgag
     2281 gccctgcttg cgcatgagct ccgcggccgc atcgaggtca cccttggtct cgacgagcgc
     2341 cttcttgcac tccatcatgc ccgcgccggt gcgctcgcgg agctgcttca ccgtatccgc
     2401 cgtgatattc atgacgcgcc tccctggcac ttactcgccg gtggagtcgc cgcgcccctt
     2461 gcggcggctc accggcgggg agacgcgccg ggcg
//
LOCUS       DATLKL010000659         3025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_165926, whole genome shotgun sequence.
ACCESSION   DATLKL010000659 DATLKL010000000
VERSION     DATLKL010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165926"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VM713_08890"
     CDS             <1..682
                     /locus_tag="VM713_08890"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HVH84418.1"
                     /translation="VFDAAAVAHAIDTAGRSVWDRDRPFTLVVLYPPLARADADAARV
                     ELERTATERGLPISVIPLTVVDSSGNTLAADSLLQSAQRYGGDQVLVGRADAAGAAAG
                     QLQWTLYTRAQSASWAGPLSAGIDHAVDLLAPPPGPVGETEAAARVRVEGVGGLADHA
                     AVARLLQSIPGVRRAEVVEVDSGSVTFEVEMRGGSAALERELAGSAHMVRSASGSASL
                     VYRYQPQG"
     gene            703..1266
                     /locus_tag="VM713_08895"
     CDS             703..1266
                     /locus_tag="VM713_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HVH84419.1"
                     /translation="MLRSSVRHLPNFICLIRIALIWPIVTALAAGAMPRALALFVVAA
                     ISDGLDGYLAKRFSWTSEVGKILDPLSDKLLLVTVFVESAWLGLVPWWLTAAVVARDV
                     MIGLGALIYRLWFGPLRGRPTVLSKMNTGLQLFYVMLIMLDAAAGVPPREILDAAAAL
                     TLATTVLSGLHYVASFTRRVWTPARQA"
     gene            1271..1978
                     /gene="hda"
                     /locus_tag="VM713_08900"
     CDS             1271..1978
                     /gene="hda"
                     /locus_tag="VM713_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HVH84420.1"
                     /translation="MQQLPLGVRLADRALFESFLPGRNAEPLAHARAVARGEARGAAW
                     LSGPGGAGKTHLLQAICAAAGVRVRAGYVPLREVAHVGVELLDGLGQLACLCLDDLDQ
                     VAGQLAWERRLFGLLREIEEAGGALIVAAQTPPALLPWALPDLGSRCTAGAVFALRAL
                     DEAEQHAALELRARLRGLELPEETWQWLRRRYPRDMRRLYELLDTLDEAALAAQRRLT
                     VPFIREVLLRRAPPPPH"
     gene            complement(1932..2561)
                     /gene="wrbA"
                     /locus_tag="VM713_08905"
     CDS             complement(1932..2561)
                     /gene="wrbA"
                     /locus_tag="VM713_08905"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HVH84421.1"
                     /translation="MSEILVLYYSRGGATAELARQVCRGVESVPGARARLRTVPQVAA
                     SSEAPPRAVPVSGPPYATLDDLRETAGLVLGSPTRFGNMAAPLKYFLDGTASLWLTGA
                     LADKPAGVFTSTQTLHGGQETTLVSMMLPLLHHGMYLVGLPYTERALNETASGGTPYG
                     ASHVGRSEQAPLLTAEEQSLAQALGRRVARLVVQLSAAAGARGAAAPRE"
     gene            2643..2915
                     /locus_tag="VM713_08910"
     CDS             2643..2915
                     /locus_tag="VM713_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HVH84422.1"
                     /translation="MICKKCVVGGRVQGVFYRATAARRAQELGIRGHARNLPDGRVEV
                     LACGDREAVETFVSWLWIGSSACKVTSVEANDAPVSTPLPGGFHTA"
BASE COUNT          400 a         1054 c         1106 g          465 t
ORIGIN      
        1 cgtgttcgac gccgcggcgg tcgcgcatgc catcgacacc gccggccgca gcgtctggga
       61 ccgcgaccgt ccctttaccc tggtcgtgct gtatccgcca ctcgcacgtg ccgacgccga
      121 cgcggcccgc gtcgagctcg agcgcacggc caccgagcgc gggctgccga tcagcgtgat
      181 cccgctgacc gtggtggaca gcagcggcaa cacgctcgcc gccgactccc tgctgcagtc
      241 ggcgcagcgc tatggcggcg accaggtgct cgtgggacgt gccgacgcgg cgggcgcggc
      301 cgccggacag ctgcagtgga cgctgtacac gcgcgcgcag agcgcgagct gggccggccc
      361 cctgagcgcc ggcatcgatc acgcggtcga tctgctggcg ccgccgccgg ggccggtggg
      421 cgagaccgag gccgccgcgc gcgtgcgcgt cgaaggcgtc ggcgggctgg ccgaccacgc
      481 ggcggtcgcg cgcctgctgc agagcattcc cggcgtgcgc cgcgcggaag tcgtcgaggt
      541 cgactccggc agcgtcacct tcgaggtgga aatgcgcggc ggttcggcgg ccctcgaacg
      601 cgagcttgcg ggctcggcgc acatggtgcg cagcgcctcc ggctccgcgt cgctcgtgta
      661 ccgctatcag ccgcagggct gagggctagg ccgctcgccg ccttgcttcg ctccagcgtg
      721 cgccacctcc ccaatttcat ctgcctgatc cgcatcgccc tgatctggcc gatcgtcacg
      781 gcgctcgccg ctggcgcgat gccgcgggcg ctcgcgctgt tcgtggttgc cgcgatctcg
      841 gacggactcg acggctatct cgccaagcgc ttcagttgga cctccgaggt cggcaaaatc
      901 ctcgatccgc tgtccgacaa gctgctgctg gtgacggtgt tcgtcgagtc ggcatggctc
      961 gggctggtgc cgtggtggct gaccgcggcg gtagtggcgc gcgatgtgat gatcggactc
     1021 ggagccctga tctaccgcct gtggttcggt ccgctgcgcg gtcgaccgac cgtgctgagc
     1081 aagatgaaca cgggactgca gctgttttac gtgatgctca tcatgctcga cgccgccgcc
     1141 ggcgtgccgc cacgggagat actcgacgct gcggcggcac tcaccctcgc gaccacggtg
     1201 ctgtcggggc tgcactacgt ggcgagtttc acgcgccggg tctggacacc ggcgcgccag
     1261 gcctgagtcg atgcaacagc tgccactcgg cgtgcggctg gcggaccgcg cgctgttcga
     1321 gagctttctg ccagggcgca atgccgagcc actcgcgcac gcgcgcgccg tcgcccgggg
     1381 cgaggcacga ggtgcggcct ggctcagcgg gcccggcggc gccggcaaga cccatctgct
     1441 gcaggcgatc tgcgccgctg ccggcgtgcg ggtgcgcgcc ggttacgtgc cgctcaggga
     1501 ggtcgcgcat gtcggggtcg agctgctcga cgggctcggg cagctcgcat gcctgtgcct
     1561 cgacgacctc gatcaggtgg ccgggcaact cgcctgggag cgccggctgt tcggcctgct
     1621 gcgtgaaatt gaggaggcgg gcggcgcgct catcgtcgcc gcgcagacgc cgccggcgct
     1681 gctgccatgg gcactgccgg accttggatc gcgctgcacg gcgggcgcgg tgttcgcgct
     1741 gcgcgcgctc gatgaggccg agcagcacgc ggcgctcgag ttgcgcgcgc ggctgcgggg
     1801 gctcgagctg cccgaggaga cctggcagtg gctgcggcgg cgctatccgc gcgacatgcg
     1861 gcgactctac gagctgctcg acacgcttga tgaggccgcc ctcgcagcgc agcgccgcct
     1921 cacggtgccg ttcattcgcg aggtgctgct gcgccgcgcg cccccgccgc cgcactgagc
     1981 tgcaccacca gccgcgcgac ccggcggccg agcgcctggg cgagcgattg ctcctccgcg
     2041 gtgagcagcg gcgcctgctc ggagcggccg acgtgcgaag cgccgtaagg ggtcccgccg
     2101 cttgccgtct cgttgagggc gcgttctgtg tagggcaggc cgacgagata catgccatga
     2161 tgcagcagcg gcagcatcat ggagacgagc gtcgtctcct gcccgccgtg cagcgtctgt
     2221 gtcgaggtga aaacgcccgc gggcttgtcc gcgagcgcgc cggtgagcca caggctcgcg
     2281 gtgccgtcga gaaaatactt cagcggcgcc gccatgttgc caaagcgcgt cgggctcccg
     2341 agcaccagtc ccgcggtctc gcgcaggtca tcgagtgtcg cgtagggcgg cccactcaca
     2401 ggcacggcgc gcggcggggc ctcgctcgat gctgcgacct gcggcacggt gcgcagccgc
     2461 gcacgcgcac ctggaaccga ctcgacgccc cggcagactt gtcgcgcgag ctccgcagtg
     2521 gcgccaccgc gggagtaata gagcacgagg atctctgaca tgttggaacc agtctccgtg
     2581 ggcgattgtc gcctagctca agtagtatta cgaatctctg ccgtgaggga cgcaagctga
     2641 gcttgatctg caagaagtgc gtggtgggcg ggcgtgtcca gggcgtcttc taccgcgcca
     2701 ccgcagcgcg acgtgcgcag gagctcggca tccgcgggca cgcgcgtaac cttccggacg
     2761 gtcgcgtgga ggtcctggcg tgcggcgacc gcgaggcggt ggagacattc gtgagctggc
     2821 tgtggatcgg ctcatccgcc tgcaaggtga cctcggtcga ggccaatgat gcaccggtga
     2881 gcacgccgct cccgggcggg tttcacaccg cttgaaaggc cagaggatgc gcaagcgcgg
     2941 atcagtggcc ctgctcgccg ctgctgcaat cgcggcagcc actgcgggcg ccacggcgcg
     3001 caccgctgca cagcccgcgc cgccg
//
LOCUS       DATLKL010000660         2578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_64466, whole genome shotgun sequence.
ACCESSION   DATLKL010000660 DATLKL010000000
VERSION     DATLKL010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64466"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..724)
                     /gene="imuA"
                     /locus_tag="VM713_08915"
     CDS             complement(86..724)
                     /gene="imuA"
                     /locus_tag="VM713_08915"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HVH84423.1"
                     /translation="MDGAPKLARLLEHPAIWRGRSAASLEVLPSGFAALDERLPGRGW
                     PRSGLIEILTARFGSGELYLLLPVLAALTRAAAARWCVWVAPPLMPFAPALAAAGIAL
                     ERVAVVGGARPLWAFEQVLGSGACDAALAWARQPKPRELRRLQLAAERGRTMGVLFRP
                     RSAAREASPAVLRLGLTPLAAGVRVTLLKGRGADRGTIELAWPEDATGRTRG"
     gene            complement(737..1360)
                     /gene="lexA"
                     /locus_tag="VM713_08920"
     CDS             complement(737..1360)
                     /gene="lexA"
                     /locus_tag="VM713_08920"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HVH84424.1"
                     /translation="MSDLTPRQTQILRLIQRFISETGMPPTRAEIAEELGFRSANAAE
                     EHLRALARKGVIALMPGTSRGIQLKDTIREQIGLPLIGRVAAGKPIFAEENFEARLAI
                     DPEVFQPRPHYLLKVTGMSMKDAGILDGDLVAVHRTSEVRSRQIVVARLENEVTVKRY
                     RQEGSIVWLLPENTEFEPIRVDLKEQPLLIEGIVVGVVRRGRASGLM"
     gene            1987..2304
                     /locus_tag="VM713_08925"
     CDS             1987..2304
                     /locus_tag="VM713_08925"
                     /inference="COORDINATES: protein motif:HMM:NF023267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HVH84425.1"
                     /translation="MKCASIVKAAAAAALVVGMAGCTDLKPLQAQVDDLKAQLAKTQS
                     DISGAKSAADAASSAASSASQAASGAQSTANQALAAAHASQSCCDATNEKIDRMFKRS
                     VSK"
BASE COUNT          470 a          842 c          838 g          428 t
ORIGIN      
        1 aatggcacga catgcgatgc gggtgtagcc gggttgggtg gcgcctcatc ggcccgcgcg
       61 ccgggtatgg ggagcgaggc catcttcagc ctcgcgtgcg tccggttgcg tcttcgggcc
      121 aggcgagctc gatcgtgccg cgatctgcgc cacggccctt cagaagagtg acgcgcacgc
      181 ccgccgcgag tggcgtgagg ccgaggcgca gcaccgccgg ggatgcttcc cgggcggcgc
      241 ttcgcgggcg aaacaacacc cccattgtcc ggccgcgttc ggcggcgagc tgcagacggc
      301 gcagctcgcg cggcttgggc tgccgggccc aggcgagcgc ggcatcgcag gcacccgagc
      361 cgagcacctg ctcgaaagcc cacagcggac gcgcaccccc gaccaccgca acacgctcga
      421 gcgcaatgcc ggcggcagcg agtgccggag cgaacggcat cagaggaggc gcgacccaca
      481 cgcaccagcg cgccgccgcg gcgcgcgtca gcgccgcgag caccggcaac agcaggtaca
      541 actcgccgct gccgaagcgc gcggtgagaa tctcgatcag tccgctgcgg ggccagcctc
      601 gaccgggcag ccgctcatcg agcgccgcaa aaccgctggg cagcacctcg aggcttgcgg
      661 cgctgcggcc gcgccagatg gccggatgct cgagcaaccg agcgagcttc ggagcgccgt
      721 ccataagcgc atgaggtcac atcaatccgc tcgcgcggcc ccggcgcacc acacccacca
      781 cgatgccttc gatgagcaac ggctgctctt tcagatccac ccgaatcggc tcgaactcgg
      841 tgttctcggg cagcagccac acgatggagc cttcctggcg atagcgtttc acggtgacct
      901 cgttctccag gcgtgccacc acgatctggc ggctccggac ctcggaggtg cggtgcacag
      961 cgaccaggtc gccatcgagg atgccggcgt ctttcatgct catgccggtg actttcagga
     1021 ggtaatgcgg ccgcggctgg aacacttccg gatcgatcgc aaggcgcgcc tcgaagttct
     1081 cctcggcgaa gatcggctta ccggcggcaa cccgtccgat gagcggcagc cctatctgct
     1141 cacggatcgt gtctttcagc tggatgccgc gggaggtgcc gggcatgagc gcgatgacac
     1201 ctttgcgggc gagcgcccgt aaatgctcct cggcggcatt ggccgaacgg aagccgagct
     1261 cctcggcgat ctcggcccgc gtaggcggca tgccggtctc agagatgaac cgctgaatga
     1321 ggcgcaggat ctgtgtctgg cggggggtca gatctgacat cttgagtgtg cctgtttata
     1381 tatacagcta ctgtaattat atccatacct gactcttcgg caagtcctgc gcgatatccc
     1441 actccctgcc ccctcttgtc cgcaggagct cgccgcgaga ccagtcaccc gacccactct
     1501 aaagattcat gcgcggctcc ttctctcccg ccaagccatc ggtcacgtct gaaggtctcg
     1561 cagcgaggag acgcgcgcac cgcgaggcgc ttcatttgcg ttgcgcgcgc cagcgcccga
     1621 tgcaccactt tcacgcacga gcgcatgcga gcgcatcacg cggaactggt gcgattacaa
     1681 attcttaaga aacactccga gactctgata acaacaccac tcgctgcgcg ccacgtgctg
     1741 cgcgtcgcgc gcgcgaatgc ctgaaattgc gctgcaaaat cgttcagtcg ccgttcagcc
     1801 aaccgcgcgg cttgccgcgg cgaacgtgag cgtcgctgtc gcgaaccgaa gacaagcgcc
     1861 gcctccacgg ttgcattccc gcggcaaatt tgctgcagtg gcgcgcggcg aagacggcgg
     1921 aacttgccca ccggagtcgc atcggttatc aaggcatccc gaatcaacaa cccaaagggg
     1981 acttgtatga agtgcgcgag cattgtgaaa gctgctgctg ccgcggcgct ggtggtggga
     2041 atggcaggtt gcacggatct gaagcccctc caggctcagg ttgacgatct gaaggctcag
     2101 ctggccaaga cgcagagcga tatatcgggc gcaaagagcg ccgctgacgc ggccagcagc
     2161 gcggcctcct ccgccagtca ggcggcgagc ggcgcgcaga gcacagccaa tcaggctctg
     2221 gctgcggccc acgccagtca gtcgtgctgt gatgcgacga acgagaagat cgaccggatg
     2281 ttcaagcggt cggtatcgaa gtaagccgct agtctctgga actgatgaat aacgccgcgc
     2341 atctgcgcgg cgtttttttt aatacggggg tcttaaggct gcagcgcgcg tcttgcagcg
     2401 atgctcgcgt cactccgtcc gcaggaagcc atgatacaga ccgctggcgt cggtgtagta
     2461 accggtgatt gcgttggaat tgttgttgct ggcgggggtg gtaccgcgag cgctggcggt
     2521 gcccgcagcc ggggcgtcga agctagtaat tatgccgtgc tcgtcgcgca gaaagccg
//
LOCUS       DATLKL010000661         3163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_143493, whole genome shotgun sequence.
ACCESSION   DATLKL010000661 DATLKL010000000
VERSION     DATLKL010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143493"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VM713_08930"
     CDS             <1..1572
                     /locus_tag="VM713_08930"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HVH84426.1"
                     /translation="DELPALDPKAYEAAARDRLADTWIQPGLPSTQAAAEPPAGAAAG
                     EDPLRTAVANLHQAQELLHSRGARLAEIERALEEAHGARAGAEQRVAQLTAELITVRA
                     AAGQQAGDLAAELAARERAQRALENELQETRARSTSYFESLQSTERHRSLFEGLLAEL
                     QEEIDTHESGRARLARELAGRDMRAAELESDLSQRAVRIAELDAQVATLGTALQQRDV
                     ELTRLRALEASLGAALEAARAEIVTGSTQAGEHEAALNEARTRVATLTAELAAERKRG
                     AGLEEELGRVRSDMEQWAGAVQAAQAERSGHLADIASGGARAQQLEERVAEQVELLRT
                     LQSASDAAMSRAREAEDDLRVAEEAIARLESQLRSRDSRVSELEKANQQWRHTLEEAR
                     AGHAETDAHEALRPALHTEAGEGAVREPAPDGATRLLIHTDGEREVVHVLGRKTSIGR
                     TPDNDLQIDAKYVSRHHAVILAGPAHTIIEDLNSTNGVIVNGRRITRQVLNDGDQVTI
                     GRAAYRFGVRRADQR"
     gene            1607..3124
                     /gene="ilvA"
                     /locus_tag="VM713_08935"
     CDS             1607..3124
                     /gene="ilvA"
                     /locus_tag="VM713_08935"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HVH84427.1"
                     /translation="MASPYIERILKARVYDVAIESPLEAAARLSRRLGNEVLLKREDL
                     QPVFSFKLRGAYNLIAHLGEAAAARGVVCASAGNHAQGVALAARRRAIPAVIVMPETT
                     PLIKVQAVIDLGAEVVLHGDDYDAALERAQTLERERNLVFVHPFDDPDVIAGQGTVAV
                     EILRQSGGRPDAIFVPVGGGGLLAGIAVYVKYLYPGVKVVGVEPRDAAGMYESLQAGR
                     RVTLERVGIFADGVAVRRVGEETFALCRGSVDEIVLTDTDEICAAIQDVFEDTRSIVE
                     PAGALAVAGLKQYVAREGWRGKRLVAINSGANMNFDRLRHVAERADIGGEREALLAVV
                     IPEQPGSFLNFCEALGKRSVTEFNYRYAGPEAAQIFVGLALSQGRAEKDAVSAQLRAA
                     GFVVTDMTDDEMAKLHVRYMVGGHARGLEHELLYRFEFPERPGALMKFLQAIGSRWNI
                     SLFHYRNHGSDYGRVLAGIQVPPGDRADFLEHLRELHYAYVEETGNPAYRMFLSS"
BASE COUNT          493 a         1119 c         1142 g          409 t
ORIGIN      
        1 gacgagcttc cggcactcga cccaaaagcc tacgaggccg ccgctcgcga tcggctggcc
       61 gacacctgga ttcagcccgg gctgccctcc acgcaggcgg ccgcagagcc acccgcgggc
      121 gccgccgccg gcgaggatcc actgcggacg gcggtggcca atctgcacca ggcgcaggag
      181 ctgctccaca gccgcggcgc gcgcctcgcc gaaatcgaac gcgcgctcga ggaggcgcac
      241 ggcgcgcgcg ccggcgctga gcagcgcgtc gcgcaattga ccgcggagct catcaccgta
      301 cgcgccgccg cgggccaaca ggccggcgac ctcgccgcgg agctcgccgc ccgggagcgg
      361 gcgcagcggg cgctcgagaa cgagctgcag gaaacgcgcg cgcgctccac gagctacttc
      421 gagtcattgc agtcgacgga gcggcaccgc tcactgttcg agggcctgct cgcggagctg
      481 caggaggaga tcgacacgca cgagagcggc cgcgcgcgcc tcgcgcgcga gctggccggc
      541 cgcgacatgc gcgccgcaga gctcgagtcg gatctcagtc agcgcgcggt acgcatcgcc
      601 gagctcgacg cgcaggtggc gacactcggc accgccctgc agcagcgcga cgtcgagctc
      661 acgcggctgc gggccctgga ggcgagcctc ggcgcggcgc tcgaggcggc gcgcgccgag
      721 atcgtgaccg ggagcacgca ggccggcgag cacgaggccg cgctcaacga ggcgcgcacc
      781 cgcgtcgcga cgctcaccgc cgagctcgcc gccgagcgca agcgcggggc cgggctcgaa
      841 gaggagctcg gccgggtacg ctcggacatg gagcaatggg ccggcgccgt gcaggcggcg
      901 caggccgaac gcagcgggca cctggccgac atcgcctccg gcggcgcgcg cgcgcagcag
      961 ctcgaggagc gcgtggccga gcaggtcgag ctgctgcgca cgctgcagag cgcctccgac
     1021 gccgccatgt cgcgcgcccg cgaggcggag gacgatctgc gcgtcgccga ggaggcgatc
     1081 gcgcggctcg agtcgcagct gcgcagtcgc gactcgcgcg tctcggagct cgagaaggcc
     1141 aaccagcagt ggcgacacac gctcgaggaa gcgcgcgccg ggcatgccga gaccgatgcc
     1201 cacgaggcgc tccgccccgc gttgcacacc gaggccgggg agggcgcggt gcgcgagccg
     1261 gcgccggacg gggcgacgcg cctcctgatc cataccgacg gcgaacgcga ggtggtgcac
     1321 gtgctcgggc gcaagaccag catcggccgc acccccgaca acgacctgca gatcgacgcc
     1381 aagtacgtga gccgccacca cgcggtgatc ctcgcgggtc ccgcgcatac catcatcgag
     1441 gacctgaaca gcaccaatgg cgtgatcgtc aacggccggc gcatcacgcg ccaggtcttg
     1501 aacgacggcg accaggtgac gatcgggcgc gcggcatacc gtttcggcgt gcggcgcgcc
     1561 gaccagcgct gacagagacg cgccgccttc gctaagctcg ctcatcgtgg cgagcccata
     1621 catcgagagg atcctcaagg cgcgcgtcta cgacgtcgcg atcgagtcgc cgctcgaggc
     1681 ggccgcgcgc ctgtcgcgcc ggctcggcaa tgaggtgctg ctcaagcgcg aggacctgca
     1741 gcctgtgttc tccttcaagc tgcgcggcgc ctacaacctc atcgcgcacc tgggcgaagc
     1801 ggccgccgcc cgcggcgtcg tatgcgcctc ggccggtaat cacgcgcagg gagtggcgct
     1861 cgccgcacgc cgccgcgcga ttcccgccgt tatcgtcatg ccggagacga cgccgctcat
     1921 caaggtgcag gcggtcatcg acctcggcgc cgaggtcgtg ctgcacgggg acgactacga
     1981 cgcagcgctc gagcgcgcgc agacgctcga gcgcgagcgc aatctcgtgt tcgtgcaccc
     2041 cttcgacgat ccggacgtca tcgctggcca gggcaccgtc gcggtggaga tcctgcgcca
     2101 gtcgggcggc aggcccgacg cgatcttcgt gccggtcggt ggtggcgggc tcctggccgg
     2161 catcgccgtg tacgtgaaat acctgtaccc cggcgtcaag gtagtcggcg tcgagccgcg
     2221 cgacgccgcc ggtatgtacg agtctctgca ggcgggccgg cgtgtcacgc tcgagcgcgt
     2281 cggcatcttc gccgacggcg tcgcggtccg ccgggtcggt gaggagacgt tcgcgctgtg
     2341 ccgcggcagc gtcgacgaga tcgtgctcac cgacaccgac gagatctgcg ccgcgatcca
     2401 ggacgtgttc gaggacaccc gctcgatcgt cgagccggcc ggggcgctcg cggtggccgg
     2461 tctgaagcag tacgtggcgc gtgagggctg gcgcggcaag cgcctggtgg cgatcaacag
     2521 cggcgccaac atgaacttcg accggttgcg tcacgtcgcc gagcgggctg acatcggcgg
     2581 cgagcgcgag gcgctgctcg cggtcgtgat cccggagcag ccgggcagct tcctcaactt
     2641 ctgcgaagcg ctcggcaagc gcagcgtcac cgagttcaac taccgctacg cgggtcccga
     2701 ggcggcgcag atcttcgtgg gtctcgcgct gtcgcagggg cgcgccgaga aggatgcggt
     2761 gtcggcgcag ctgcgcgccg ccggcttcgt cgtcaccgac atgaccgacg acgagatggc
     2821 gaagctgcac gtgcgctaca tggtgggcgg gcatgcgcgc ggcctcgagc acgagctgct
     2881 ctatcgcttc gagttcccgg agcgccccgg ggcgctcatg aagttcctgc aggcgatcgg
     2941 ctcgcgctgg aacatcagcc tgttccacta ccgcaatcac ggctcggact acgggcgggt
     3001 gctggccggg atccaggtgc cgcccggtga ccgtgcggat ttcctcgagc acctgcgcga
     3061 gctccattac gcctacgtcg aggagaccgg caacccggcc tatcgaatgt tcctttctag
     3121 ttgaattggc tgaaatcgcc tgcggcgatt tcagcggaca tca
//
LOCUS       DATLKL010000662         2214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_7735, whole genome shotgun sequence.
ACCESSION   DATLKL010000662 DATLKL010000000
VERSION     DATLKL010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7735"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VM713_08940"
     CDS             <1..743
                     /locus_tag="VM713_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVH84428.1"
                     /translation="THSSGNHAAALARAARLRGIPAYIVMPENTPRAKRAAVERQGAV
                     ITLCEPTLAARERCAAEVTARTGATMVHPYDDLSVMAGQGTVGVELLEQAPDLDVLLA
                     PVSGGGLLSGIAVAARTLRPAVRVVGVEPSGADDAARSLRAGRIVPCERPTTIADGLR
                     ATIGVRPFAEFRRYVDDIITVSDAAIVSAMRHIWEVMKIIVEPSGAAAYAAVAAGRLE
                     LRGRRVGVVLSGGNLDLESLPWITPARE"
     gene            complement(724..1929)
                     /gene="pcaF"
                     /locus_tag="VM713_08945"
     CDS             complement(724..1929)
                     /gene="pcaF"
                     /locus_tag="VM713_08945"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HVH84429.1"
                     /translation="MSEAYICDAVRTPIGRYGGMLAAVRTDDLAALPIAALLKRNTAL
                     DPQGVDEVLLGCANQAGEDNRNVARMAALLAGLPVSVPGATVNRLCGSGLDALAMGAR
                     AVRLDEARLVVAGGVESMSRAPFVLPKAETPFTRAAEIYDTTIGWRFVNPRMRERYGI
                     DSMPETGENVAAEYGISRADQDAFALRSQQRAGAAYARGFFAAELVPVEIPQRKGKAL
                     VVERDEHPRPETTLEQLAALPTPFRSGGTVTAGNASGVNDGACALLIASEEAARRHGL
                     TPRARIVAAATAGVAPRIMGMGPVPATRKVLERCGLAIGQIDVLELNEAFASQALAVL
                     RELKVADDAAHVNPNGGAIALGHPLGMSGARLATTAVSQLLATRGRYALCTMCIGVGQ
                     GIAVILERV"
BASE COUNT          300 a          778 c          798 g          338 t
ORIGIN      
        1 ccacccactc ctcgggcaac cacgcggccg cgctggcgcg cgcggctcgc ttgcgcggca
       61 tccccgccta catcgtcatg ccggagaaca cgccgcgcgc caaacgcgcc gcggtcgagc
      121 gccagggcgc cgtaattacc ctctgtgagc cgacgctcgc agctcgtgag cgctgcgccg
      181 ccgaagtgac cgccaggacc ggcgccacca tggtccaccc ctacgacgac ctgagcgtga
      241 tggccggaca ggggacggtc ggcgtcgagc tgctcgagca ggccccggac ctcgacgtgc
      301 tgcttgcgcc ggtcagtggc gggggattgc tgagcggcat tgcggtcgcc gcgaggaccc
      361 tgcggcccgc ggtgcgggtg gttggagtcg agccgagcgg tgcggacgat gcggcacgct
      421 cgctgcgcgc gggcaggatc gtgccctgtg agcggcccac gaccatcgct gatggcctgc
      481 gggccacgat cggcgtgcgg ccgttcgcgg aatttcgccg ctacgtcgat gacatcatca
      541 ccgtctccga cgcggctatc gtgagcgcga tgcgccacat ctgggaagtg atgaagatca
      601 tcgtcgagcc ctcgggcgcc gcggcgtacg cggctgttgc tgccgggcgg ctcgagctac
      661 gcggccggcg cgtgggcgtg gtgttgagtg gcgggaacct cgacctcgag tctcttccct
      721 ggatcacacc cgctcgagaa tgactgcgat tccctggccg acgccgatgc acatggtgca
      781 gagtgcgtag cggccgcgtg tcgcgaggag ttggctcacc gccgtggtgg cgaggcgcgc
      841 tcccgacatc cccagcgggt ggccgagcgc gatggcgccg ccgttcgggt tcacgtgcgc
      901 ggcgtcgtcg gcgaccttga gctcgcgcag caccgcgagg gcctgggagg cgaaggcctc
      961 gttgagctcg aggacgtcga tctgaccgat ggcgaggccg cagcgctcga gcactttgcg
     1021 cgttgcgggc accggaccca ttcccatgat gcgcggcgcg acaccggcgg tagccgccgc
     1081 gacgatgcgc gcgcgcggcg tgagcccgtg gcgccgtgcg gcctcctccg aggcgatgag
     1141 cagcgcacac gcgccgtcgt tgacgcccga ggcattgccc gcagtgaccg tgccgccgct
     1201 acggaacggg gtggggagcg cggcaagctg ctcgagcgtc gtctctggcc ggggatgctc
     1261 gtcgcgctcc accaccagag ctttaccctt gcgctgcggg atctcgaccg ggacaagctc
     1321 ggcggcaaag aagccgcgtg cgtaggcggc gccggcgcgc tgctggctgc gcagcgcgaa
     1381 tgcatcctgg tcggcgcgcg agatgccgta ttcggccgcg acgttttcgc cggtctccgg
     1441 catcgagtcg atcccatagc gctcgcgcat ccgcggattg acgaagcgcc agccgatggt
     1501 ggtgtcgtag atctcggctg cgcgggtgaa cggcgtttcc gccttcggca gcacgaacgg
     1561 cgcacgcgac atcgactcga cgcctccggc gacgacgagc cgcgcctcgt cgaggcgcac
     1621 ggcgcgcgcg cccatcgcca gcgcatcgag gccggaaccg cacaggcggt tgacggtggc
     1681 ccccggtacc gacaccggca agccggcgag caaggccgcc atgcgcgcca cattgcggtt
     1741 gtcctcgcca gcctgattgg cgcagccgag cagcacctcg tccacgcctt gcgggtcgag
     1801 cgccgtgttg cgcttgagca gtgccgcgat cggcagcgcc gcgaggtcgt cggtgcgcac
     1861 ggccgcgagc atcccgccgt agcggccgat cggggtgcgc accgcatcgc agatgtaggc
     1921 ctcgctcatg cgcgcatcat atcgcctccc gcggcgtacc ggcggcgcgc cggtacagtg
     1981 cgcggcggcg gtggcgcgcg cgtatctgcc gccgggccgc ggtgctgagc gaagcttcgc
     2041 gtatgctcaa cccgctgccg tttgctacct attcctttgt catgtggctc actcccgggc
     2101 ccaacaacgt gatgctgaca gcctcgggcg ccaacttcgg gctgcggcgc accctgccgc
     2161 acatgctcgg catctgcgcg ggattcgcgc tgcagctgct cgccgtgtgc gccg
//
LOCUS       DATLKL010000663         1550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_109456, whole genome shotgun sequence.
ACCESSION   DATLKL010000663 DATLKL010000000
VERSION     DATLKL010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109456"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VM713_08950"
     CDS             complement(<1..537)
                     /locus_tag="VM713_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84430.1"
                     /translation="MSEALYPPPRPQSVGEVLDLSFRIYRRTFVKCLVLGGLVVVTQW
                     LPNLYAIARGQTVVQSLLSPSVDPLRTLITLAAGLLALIFPVAIIYRQYRIITGEESG
                     GELLRALRLLGRIVLIVILYALLMVACLLLVVPAAFVSGITQYLLFAVLLLPLAYVLL
                     RLACAFTAMVIENTGAAAS"
     gene            572..1426
                     /gene="prmC"
                     /locus_tag="VM713_08955"
     CDS             572..1426
                     /gene="prmC"
                     /locus_tag="VM713_08955"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HVH84431.1"
                     /translation="MTAATVAVLLAQGTAALAGAAGAQGATAGLEAELLLAHVLAAPR
                     ARLKSHPETAVAATAAQRYRELIRRRAGGEPLAYLTGRREFWSLELAVTPAVLIPRPE
                     TELLVERALILGKERAARVADLGTGSGAIALALARERPDWRIVATDVSPPALAVARAN
                     AAALGVTSIEFLAGNWFEPLAAARFELIVSNPPYVAADDPALADTGLAFEPPLALTPA
                     AGDALAALRLLARGAGAHLEPGGWLLLEHGASQGPEVRAELVAAGFAHVRSHQDLAGH
                     ERMTEGQR"
     gene            1423..>1550
                     /locus_tag="VM713_08960"
     CDS             1423..>1550
                     /locus_tag="VM713_08960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVH84432.1"
                     /translation="MIRFETSHGAFTVELFPKEAPVTVENFLKYVDDGFFDGTIFHR"
BASE COUNT          254 a          547 c          537 g          212 t
ORIGIN      
        1 gctcgccgcg gccccggtat tctcgatcac catcgcggtg aaggcgcacg ccaggcgcag
       61 cagcacgtag gccaggggca gcagcagcac cgcgaagagg aggtactgcg tgatgccgct
      121 cacgaaggcg gcaggcacga cgagcagcag gcaagccacc atcagcaagg cgtacaggat
      181 cacgatgagc acgatacggc cgagcagacg cagcgcgcgc agcagttcgc cgccactctc
      241 ctcgccggtg atgatgcggt actgccggta gataatggcg accggaaata tcagcgcgag
      301 cagccccgcg gccagagtga tgagggtgcg gagcggatcg accgagggcg acagcagcga
      361 ctgcacaacc gtctgcccgc gtgcgatcgc gtagaggttt ggcagccact gcgtcaccac
      421 cacgaggccg cccaacacca ggcacttgac gaaggtgcgc cggtagatgc ggaacgaaag
      481 gtcgagaacc tcgccaacgc tctgcggacg tggcggcgga tacaatgctt ctgacatcgg
      541 aacatcttag cgctgttgcg cacgggagcc tatgacggcc gccaccgtcg cggtgctgct
      601 cgcccagggc accgctgcac tggctggcgc cgccggtgcg cagggagcga ctgccggcct
      661 cgaggcggag ctactgctcg cccacgtcct cgccgcgccg cgtgcgcgcc tcaagtcgca
      721 cccggagacg gcggtggcgg ccacggccgc ccagcgctat cgcgagctca tccggcggcg
      781 cgccggcggc gagcccctgg cctacctcac cggccggcgg gagttctggt cgctcgagct
      841 cgcggtgacc cccgcggtac tgatcccgcg gccggagacg gagctgctgg ttgagcgcgc
      901 gctcattctc gggaaggagc gcgcggcccg ggtggccgac ctcgggaccg gctccggcgc
      961 catcgccctc gcgctggcgc gcgagcggcc cgactggcgc atcgtcgcga cggatgtctc
     1021 gccgcccgcg ctcgccgtgg cacgcgccaa tgctgcagcc ctcggcgtga ccagcatcga
     1081 gttcctcgcg ggcaactggt tcgagccgct cgccgcggca cgcttcgaac tcatcgtgag
     1141 caacccgccc tatgtcgccg cggacgaccc ggcgctcgcg gacacgggcc tcgccttcga
     1201 gccgccgctt gcgctcacac cagccgcggg agatgcgctc gcggcactgc ggctgctggc
     1261 gcgcggcgcc ggggcgcacc ttgagccggg cggctggctg ctgctcgagc acggcgcgag
     1321 ccaggggccc gaggtacgcg ccgagcttgt cgccgccggc ttcgctcacg tacgctcgca
     1381 tcaggatctc gcgggacacg aacgcatgac ggaaggacaa cgatgatcag gtttgaaacc
     1441 tcgcacggcg ccttcacggt ggagctgttt ccgaaggagg cgcccgtcac ggtcgagaat
     1501 tttctcaagt acgtcgacga cggcttcttc gatggcacca tcttccaccg
//
LOCUS       DATLKL010000664         3508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_165973, whole genome shotgun sequence.
ACCESSION   DATLKL010000664 DATLKL010000000
VERSION     DATLKL010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165973"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VM713_08965"
     CDS             <1..267
                     /locus_tag="VM713_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84433.1"
                     /translation="SSEQDSFAHGLLDWPQYTRPEVFEGRAVPPVLLSGDHAGVGRWR
                     LKCAVERTWRRRPDLLARARLAPEAQRALNELLATPAAEEDRGT"
     gene            269..613
                     /gene="rplS"
                     /locus_tag="VM713_08970"
     CDS             269..613
                     /gene="rplS"
                     /locus_tag="VM713_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HVH84434.1"
                     /translation="MNKIIQDLDKRRMTRKVPDFKPGDTVVVQVKVVEGDRERVQAYE
                     GVVIARSNRGLNSSFTVRKISHGEGVERVFQTYSPAISDIMVKRRGSVRRAKLYYLRD
                     LAGKAARIEEKI"
     gene            672..2507
                     /gene="sppA"
                     /locus_tag="VM713_08975"
     CDS             672..2507
                     /gene="sppA"
                     /locus_tag="VM713_08975"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HVH84435.1"
                     /translation="MNVRSFFAGLWRGLDGLRKALHLIVLLVIFGAALGVLLSGTGRI
                     PARAALLIEPDGELVEQLPGDPFTRALEAARGDRRHETLLWDLTDSIRAAAADRRIPA
                     IALDLDKFEGGTQPSLEELARALRAFRASGKKIVAYGSELSQEGYYLAAQADETYLDP
                     LGFVLIDGYDRYRMYLKEVLDKLGVDINVFRVGQFKSAVETYTRTNMSPEDREESRAY
                     LGVLWTTYQEAIARARKLAPDAVAKYVAGLAKTVPAAGGNAAHVALEAGLVSGIKTQL
                     EVEKRLSGLVGEDKSTGSFRAVSVADYLSYAQAQRRLRASGKPHVGVIVASGEILDGD
                     QPPGTIGGDSTARLIRQARLDSDIKALVLRVDSPGGSVMASEEIYRELQALHAAGKPL
                     IVSMSGYAASGGYYISAPADEIWASPATITGSIGIFAIIPTVDRTLGKVGVSVDGVGT
                     TPLSGQLRIDRPLGAEARALLQSQIERGYDEFLERVSSGRKKSRADIDAIAQGHVWAG
                     TDAQRLGLVDHLGSFDEAVKAAASRARLSDYAPVFLEPELTWTQQLALSFKTRLVGAL
                     FGASADEVALRRLAERLDPIAREVARLSRFSVPNHVYAYCFCEVR"
     gene            complement(2508..3221)
                     /locus_tag="VM713_08980"
     CDS             complement(2508..3221)
                     /locus_tag="VM713_08980"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVH84436.1"
                     /translation="MQEHCRRRCQPSGCLRRVPGDPGLNAFKLPDSIPDDIASILDPF
                     GNATHTALTFNMVGEDVLITGAGPIGIMATAIARFTGARHIVVTDVNDYRLELARKMG
                     ATRAINVQRESLDQTMKELGILGGWDVGLEMSGNAAAFREMLRTMFHGGSVAILGIPP
                     EDMAIDWNRVIFKGLTLKGIYGREMFETWYKMASMLQSGLKIDPIITHHFPIQEFQKG
                     FETMGSGKSGKVILDWSSL"
     gene            complement(3149..>3508)
                     /locus_tag="VM713_08985"
     CDS             complement(3149..>3508)
                     /locus_tag="VM713_08985"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVH84437.1"
                     /translation="PGIWLEDIPEPKIGHNDVLIEVAKTAICGTDMHIYNWDAWAQKT
                     IPVPMAVGHEYCGRIVEVGSEVKGLKAGDRVSGEGHITCGYCRNCRAGRRHLCKNTVG
                     VGVNRPGAFAEYLAIPA"
BASE COUNT          556 a         1160 c         1217 g          575 t
ORIGIN      
        1 tcgagcgagc aggattcgtt cgcgcacggg ctcctcgact ggccgcagta cacacggccg
       61 gaggtgttcg agggccgcgc ggtgccgccg gtgctattgt cgggtgacca cgctggcgtc
      121 ggccgctggc ggctgaaatg cgcggtcgag cggacctggc ggcgccggcc cgacttgctc
      181 gcccgggcga ggctcgcccc ggaggcgcag cgtgcgttga atgagctgct ggcaacgccg
      241 gcagcagaag aggatcgagg tacgtgacat gaacaagatc attcaggatt tggacaagcg
      301 gcgcatgacc cgcaaggtgc cggacttcaa gcccggcgat accgtggtcg tgcaggtgaa
      361 agtggtcgag ggtgaccgcg agcgcgtgca ggcctacgag ggtgtcgtga ttgcccgcag
      421 caaccgcggg ctcaactcct cgttcaccgt gcgcaagatc tcccacggcg aaggggtgga
      481 gcgcgtgttc cagacctaca gcccggcgat cagcgatatc atggtcaagc gccgcggcag
      541 cgtgcgccgc gccaagctgt actatctgcg cgatctcgcc ggcaaggcgg cgaggatcga
      601 ggagaagatc tgaaggcgcg caccgcggcg gccggcgctc ggccgctgac ggcgcgcgaa
      661 gcggtgagtc cgtgaacgtg cgttcgttct tcgcgggcct gtggcgcggc ctggacggcc
      721 tgcgcaaggc tctgcacctg atcgtgttgc tggtgatctt cggggccgca ctcggcgtgc
      781 tgctctccgg gaccggccgt atcccggcgc gtgcggcgct gctcatcgag cccgacggcg
      841 agctggtcga gcagcttccc ggcgacccgt tcacgcgcgc cctcgaggcg gcgcgcggtg
      901 accgccgcca cgagacgctg ctctgggatc tcaccgactc gatccgcgcc gcagccgccg
      961 accggcgcat cccggcgatc gcgctcgacc tcgacaagtt cgaggggggc actcagccct
     1021 cactcgaaga gctggcgcgc gcgctgcgcg ccttccgcgc gagcggcaag aagatcgtgg
     1081 cctacggcag cgagctcagc caggaaggct actacctcgc ggcgcaggcg gacgagacct
     1141 acctcgaccc gctcgggttc gtgctgatcg acggctacga ccgctaccgc atgtacctca
     1201 aggaagtgct cgacaagctc ggcgtggaca tcaatgtatt ccgcgtgggg cagttcaaga
     1261 gcgccgtcga gacctacacg cgcaccaaca tgtcgccaga ggaccgcgag gagagccgcg
     1321 cctacctcgg cgtcctgtgg acgacctacc aggaggcgat tgcccgcgcg cgcaagctcg
     1381 ctcccgacgc ggtggcgaag tacgtcgctg gacttgcgaa gacggtgccg gccgctggcg
     1441 gcaatgccgc gcatgtcgcg ctcgaggccg ggctcgtcag cggcatcaag acgcagctcg
     1501 aggtcgagaa gcgcctgagc gggctcgtcg gggaggacaa gtcgacgggc tcgttccgcg
     1561 ccgtctcggt ggccgactac ctgagctatg cgcaggcgca gcgccggctg cgcgcgagcg
     1621 gcaagccgca cgtcggcgtc atcgtcgcct cgggcgagat cctcgacggc gaccagccgc
     1681 ccggcacgat cggcggcgat tcgaccgccc gtctcatccg tcaggcccgt ctcgactccg
     1741 acatcaaagc gctggtgctg cgggtcgata gtcccggcgg gagcgtgatg gcgtccgagg
     1801 agatttaccg ggagctgcag gcgctgcatg ccgccggcaa gccgctgatc gtgtccatga
     1861 gcggctacgc ggcctcaggc ggctactaca tttcggcgcc cgcagacgag atctgggcga
     1921 gcccggcgac catcaccggc tcaatcggca tctttgcgat cattccgacg gtcgatcgga
     1981 cgctcggcaa ggtcggcgtg agcgtggacg gcgtcggcac cacgccgctc tcggggcagc
     2041 tgcgcattga ccggccgctc ggcgccgagg cgcgcgcact gctgcagtcg cagatcgagc
     2101 gcggctacga cgagttcctc gagcgcgtct cgagtgggcg caagaagagc cgcgccgaca
     2161 tcgacgcgat cgcccagggc cacgtgtggg ccggcaccga cgcccagcgt ctgggcctcg
     2221 tcgatcacct cggctccttc gacgaggcgg tcaaggcggc cgccagtcgc gcccggctca
     2281 gcgactatgc gccggtattt ctcgagcccg agctcacctg gacgcagcag ctggcactgt
     2341 cgttcaagac gcgtctggtc ggcgcgctct tcggcgcgag tgccgatgag gttgcgctgc
     2401 ggcgcctcgc cgagcgcctc gacccgatcg cgcgcgaggt ggcgcgccta agccgcttca
     2461 gcgtgccgaa ccacgtgtac gcctactgct tctgcgaagt gcgttgacta caagctactc
     2521 cagtcgagga tgaccttccc cgacttgccc gatcccatgg tctcgaaacc cttttggaat
     2581 tcctggatcg ggaagtgatg ggtgatgatc gggtcgatct tgaggcccga ctgcagcatg
     2641 ctcgccattt tgtaccaggt ctcgaacatc tcccggccgt agatgccctt gagggtcagg
     2701 cccttgaaga tcacccgatt ccagtcgatc gccatgtcct cgggcgggat accgaggatc
     2761 gcgaccgagc cgccgtgaaa catggtgcgc agcatctcgc gaaacgctgc cgcgttgccc
     2821 gacatctcga gcccgacgtc ccaaccccca agtatcccga gttccttcat ggtctgatcg
     2881 agtgactcgc gctgcacgtt gatggcgcgg gtcgcgccca ttttgcgcgc gagctcgagg
     2941 cgatagtcgt tgacgtcggt gaccacgatg tgccgcgcgc cggtgaagcg cgcgatcgcg
     3001 gtcgccatga tgccgatcgg gccggcgccg gtgatcagca cgtcctcgcc gaccatgttg
     3061 aaggtgagcg cggtgtgcgt ggcgttgccg aaggggtcga gaatggaggc gatgtcgtcc
     3121 gggatcgagt ccgggagctt gaaggcgttt aggccgggat cgccaggtac tcggcgaagg
     3181 cacccggacg gttgacaccg acgccgacag tgttcttgca caggtggcgc cggccggcgc
     3241 ggcaattgcg gcagtagccg caggtgatgt ggccttcgcc cgagacgcgg tcgccggcct
     3301 tgagtccctt gacctccgag cccacctcaa cgatgcgccc gcagtattcg tgcccgaccg
     3361 ccatgggcac cgggatggtc ttctgcgccc aggcgtccca gttatagatg tgcatgtcgg
     3421 tgccgcagat cgcggtcttg gcgacctcga tcagcacgtc gttgtgcccg atcttcggct
     3481 cggggatgtc ctcgagccag atgccggg
//
LOCUS       DATLKL010000665         1584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_199653, whole genome shotgun sequence.
ACCESSION   DATLKL010000665 DATLKL010000000
VERSION     DATLKL010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199653"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VM713_08990"
     CDS             <1..1034
                     /locus_tag="VM713_08990"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HVH84438.1"
                     /translation="IAKFDLASGHCPQTFALGYKELWQLPAGRVRPGRIEHALGWPAD
                     SKTYAGSFLYHLDKDRVYVGYIVGLDYRDPRLKPFEAFQQYKNHPSVKALLEGGEILS
                     AGARTIAAGGWQSTPTLEMPGAILVGDAGGTLNFAKIKGVHQAIRSGRLAGEHIAESG
                     GGAGFDARWRASEGGRELKRVRNFKPAFKRGMWLGLANSAWEMLTAGHSPWTLKNSAD
                     WSALAKLDEFVSPERHWGERTLPPRDRVSFVFFAGNSHEESQPVHLKVADTSICAGRC
                     ATEFGNPCERFCPANVYEMVDDGRGGRRLQINAANCVHCKACDIKDPYQIITWTTPEG
                     GSGPNYQSL"
     gene            1058..>1584
                     /locus_tag="VM713_08995"
     CDS             1058..>1584
                     /locus_tag="VM713_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVH84439.1"
                     /translation="MSELWRPSAQRIANANLTRFMRCVTARTGTPLADFEATYAWSLE
                     KPTQFWSELARFADVRADWGAGPVLEHPTAMPGARFFPAAHLNFAENLLKFADDHPAL
                     IFRNERGVRRALSGRELRAEVARVAAGLGAAGVVAGDRVAGYLPNLPEAAIAMLATAS
                     IGAVWSSCSPDFGVHG"
BASE COUNT          252 a          555 c          533 g          244 t
ORIGIN      
        1 tgatcgcgaa gttcgatctc gcgagcggcc attgcccgca gaccttcgcg ctcggctaca
       61 aggagctgtg gcagctgccc gccgggcgcg tgcgcccggg gcgcatcgag catgcgctcg
      121 gctggccggc ggactcgaag acctacgccg gcagcttcct ctatcacctc gacaaggacc
      181 gggtgtacgt cggttacatc gtcgggctcg actaccgcga tccgcgcctc aagcccttcg
      241 aggccttcca gcagtacaag aatcatccga gtgtcaaggc gctgctcgag gggggcgaga
      301 tcctctcggc cggcgcgcgc accatcgcgg ccggtggctg gcagtcgacc ccgacgctcg
      361 agatgcccgg cgcgatcctc gtcggcgatg cgggcggcac gctcaacttc gccaagatca
      421 agggcgtgca ccaggcaatc cgctcaggaa ggctggccgg cgagcatatc gccgagagcg
      481 gcggcggcgc gggcttcgat gcgcgctggc gggcttcgga gggcggacgc gagctgaaga
      541 gggtgcgtaa cttcaagccc gcgttcaagc gcggcatgtg gctcggcctc gccaactccg
      601 cgtgggagat gctcaccgcc ggccactccc cctggacgct gaaaaacagc gccgactggt
      661 cggccctggc gaagctcgat gagttcgtct cgccggaacg ccattggggc gagcgcaccc
      721 tgccgccccg cgaccgggtc tcgttcgtgt tcttcgccgg caactcccac gaggagagcc
      781 agccggtgca cctcaaggtg gcggacacga gcatctgcgc cggccgctgc gccaccgagt
      841 tcggcaatcc ctgcgaacgt ttctgtccgg cgaatgtcta cgagatggtg gacgacggcc
      901 gcggcggcag gcgcctgcag atcaacgcag ccaactgcgt gcactgcaag gcctgcgaca
      961 tcaaggatcc ctaccagatc atcacctgga ccacgcccga gggaggctcg ggtccgaact
     1021 accagtcgct gtaggccgca gtaaacctca agcagccatg tccgagctct ggcgcccgtc
     1081 cgcacaacgc atagccaacg cgaatctgac gcgcttcatg cgctgcgtga ccgcccgcac
     1141 cggtacgccg ctcgccgact tcgaggcaac ttacgcctgg tcgctcgaga agccgacgca
     1201 gttctggagc gagctcgcgc gcttcgccga cgtgcgcgcc gactggggcg cgggtccggt
     1261 gctcgagcat ccgacggcga tgcccggcgc gcgcttcttc cccgctgcgc acctcaactt
     1321 cgccgagaac ctgctcaaat tcgccgacga tcatccggcg ctcatcttcc gtaacgagcg
     1381 cggtgtgcgg cgcgcgctct ccgggcgcga actgcgcgca gaagtggcgc gcgtggccgc
     1441 cgggctcggg gccgcgggcg tcgtcgccgg cgatcgcgtc gccggctacc tgccgaacct
     1501 gccggaagcg gcgatcgcga tgctggcgac agcgagtatt ggcgccgtat ggtcgtcgtg
     1561 ctcgccggac ttcggcgtgc atgg
//
LOCUS       DATLKL010000666         4524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_165977, whole genome shotgun sequence.
ACCESSION   DATLKL010000666 DATLKL010000000
VERSION     DATLKL010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165977"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VM713_09000"
     CDS             complement(<1..1025)
                     /locus_tag="VM713_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH84440.1"
                     /translation="MNISRPFIERPVATTLLMAGVFLVGLAAFPFLPVAPLPQVDFPT
                     ILVSAQLPGASPEIMASSVAQPLETQFAQIPGVAQMTSTSVLGTSQITLQFNLDRNID
                     AAAQDVQTAIDAAGGQLPKNLPAPPNQRKVNPADSPILILAVHSDVLPVTTVDDYAET
                     VIAQQLSQLPGIAQVTVGGQQKPAVRVQIDPVKLAAVGLQLEDVANLITTATVDAPQG
                     AITGPEKNFTIYDNDQLLKAAPWNDVIVAYRKGAPIRIRDIGVAVDGPENSQVRGFQN
                     GRPGVLLLIHKQPGTNVIDAVERVKAALPRVMASVPPSLKVDQVIDRTITIKASVEDV
                     ELSLLIA"
     gene            complement(1022..2227)
                     /locus_tag="VM713_09005"
     CDS             complement(1022..2227)
                     /locus_tag="VM713_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVH84441.1"
                     /translation="MKLPPLPLGSARQKAIAGFVALCVLLAAYWLFHRPATVPKPPPA
                     IPVDLATAARADVPQYLEGLGTVQAFYTVTVTARVDGQIERVAFREGQDVKKGALLVQ
                     IDPRPYQAALGMALATRDKDRALLENARRDLERYEQLAPEDLASKQTVDTQKALIAQL
                     TAQVKGDAASIDNARTQLDYTTITSPIDGRTGIRLVDPGNNVRATDTTGMVVVTQLEP
                     ISVILSLPEEDFGQLSTALAGGAVTASALSREGREELDRGTVALIDNQIDQTTGTIRV
                     KATMPNTQRRLWPGEFVNVRVLTAIRHQVLTIPAGALERGPEGLFAYLVQPDSTVAVA
                     PVEASEPVGGLVVIEKGLKAGDQVVTSNHYRLQPGAHIRANTAASAAGAAPRPLPGAT
                     SGAVAEPAS"
     gene            complement(2224..3672)
                     /locus_tag="VM713_09010"
     CDS             complement(2224..3672)
                     /locus_tag="VM713_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVH84442.1"
                     /translation="MRAWLPRGGKIRGRMSCPRSLAGLLWCSALLVTACSLGPRYHKP
                     DIAPPDHWVTASDAAVSEWPTTEWWRGFGSTELDTLISEAQGANDDLRAAVARVQQAD
                     AQVRIAGAPLLPALALSAQATRARAAVPPNGAYASADNFSPLLVASYQLDFFGKNRAL
                     RAAAAASAHASRYDRTTVELTVMAGVATTYFQALELRDRLGIADSNLANATQVLRGLK
                     LEQSAGIATALDVAQQETVVATVNAGIPPLRQQLRQSLDALAILIGRIPEAVDVTSGG
                     LDSLSQPPVKPGLPAGLLARRPDVAAAEAQLIAANADIAAARAAIFPTINLTAEGGYE
                     SPALATLLTPASRIWSLGAGLTQPVFEGGALLGGYSLARAQYRELLADYHKAVISALA
                     NVEDALIAVQQTTEQVSRQQEATGQARRAYRFAQAQMHAGTINVLTLLNTETSLFTAE
                     DALAQAKYAHLQSLVSLYQALGGGWQQKDERL"
     gene            3805..4284
                     /locus_tag="VM713_09015"
     CDS             3805..4284
                     /locus_tag="VM713_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVH84443.1"
                     /translation="MSIKLTVNGAARELDVDPDMPLLWAIRDHLELSGTKFGCGMALC
                     GACTVHIDGQPTRSCVTPVSAVAGKSVTTIEGLATPAAKAVQQAWIALDVPQCGYCQS
                     GQIMSAAALLGANPQPSDADIDAAMAGNICRCATYVRIRAAIHQAATALAAPRPQGS"
     gene            4288..>4524
                     /locus_tag="VM713_09020"
     CDS             4288..>4524
                     /locus_tag="VM713_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HVH84444.1"
                     /translation="MYVRYLEEAGRTRGPASSLTRRQFLELTTLAGSGLTLGILLPGC
                     GSAGSGGGAAAAATPLEMPFVRIAPDNTVTVLCKH"
BASE COUNT          659 a         1483 c         1646 g          736 t
ORIGIN      
        1 atggcgatga gcagcgacag ctcgacgtcc tcgaccgagg ctttgatggt gatcgtgcgg
       61 tcgatcacct ggtcgacctt gagcgacggc ggcaccgaag ccatgacgcg cggcagagcg
      121 gctttgacgc gctcgaccgc atcgatcacg ttggtacccg gttgcttgtg gatcagaagc
      181 agcacgccgg gccggccgtt ctgaaaaccc cgcacctggc tgttctcggg gccgtcgacg
      241 gcaacgccga tgtcccggat acggatcgga gcgcccttgc ggtaggcgac gatgacgtcg
      301 ttccagggcg cggccttcag cagctggtcg ttgtcgtaga tggtgaagtt cttctcgggc
      361 cccgtgatgg cgccctgcgg cgcatcgacc gtggcggtcg tgatgagatt agcgacgtcc
      421 tcgagctgca gtcccacggc ggcgagcttc accggatcga tctgcacgcg caccgcgggc
      481 ttctgctgtc cgccgaccgt cacctgcgcg atacccggca gttgcgacag ctgctgcgca
      541 atcacggtct ccgcgtagtc atcgaccgtc gtgaccggca gcacgtcgga gtgcaccgcg
      601 agaatcagaa tgggggagtc ggccggattg acttttctct gattgggcgg cgccggcagg
      661 ttcttcggca gctgcccgcc ggcggcatcg atcgccgtct gcacgtcctg ggcggcggcg
      721 tcgatattgc ggtcgaggtt gaactgcagc gtgatctgcg aggtgccgag cacgctggtc
      781 gaggtcatct gcgcgacgcc cgggatctgc gcaaactgcg tctcgagggg ctgcgcgacc
      841 gaggaggcca tgatctccgg gctcgctccc gggagctgag cgctcaccag gatggtcggg
      901 aagtcgacct gcggcagcgg cgccacgggc aggaacggaa aagccgcgag ccccaccagg
      961 aatacgcccg ccatcagcag ggtcgtcgcg accgggcgct cgatgaaggg ccgggagatg
     1021 ttcacgacgc gggctcggca accgcgccgc tggtggcgcc cggaagcggc cggggcgcgg
     1081 cgccagcggc gctcgctgct gtgttggcgc ggatgtgcgc gccgggctgc agccggtagt
     1141 gattgctcgt gaccacctgg tcgccagcct tcagcccctt ctcgatgacg accaggccgc
     1201 cgacgggctc gctcgcctcg accggcgcca cagccacggt ggagtccggc tgtaccaggt
     1261 aggcaaacag tccctcgggt ccgcgctcga gcgcgcccgc gggaattgtg agcacctgat
     1321 gacggatcgc ggttagcacg cgcacgttga cgaactcacc gggccagagg cggcgctggg
     1381 tgttgggcat ggttgccttg acgcggatcg ttccggtcgt ctggtcgatc tggttgtcga
     1441 tgagcgcgac cgtcccgcga tcgagctcct cgcgaccctc gcgcgacaac gcgcttgccg
     1501 ttaccgctcc gcccgcgagc gccgtgctca gttgtccgaa gtcctcctcc ggcaggctca
     1561 ggatcacgga gattggctcg agctgcgtca ccaccaccat cccggtggtg tcggtggcac
     1621 ggacgttgtt gccgggatcg acgaggcgga tgccggtgcg cccgtcgatc ggggaggtga
     1681 tggtggtgta gtcgagctgg gtgcgggcgt tgtcgatgga cgccgcgtca cccttgacct
     1741 gcgcggtgag ctgcgcgatg agcgccttct gtgtgtcgac ggtctgcttg ctcgcgaggt
     1801 cctcgggggc gagctgctcg tagcgctcga gatcgcgccg cgcgttctcg agcagcgcac
     1861 ggtccttgtc gcgcgtcgcg agcgccatac cgagggccgc ctggtagggg cgcgggtcga
     1921 tctgcacgag gagcgcgcct ttcttcacat cctgcccctc gcggaacgcg acgcgctcga
     1981 tctgaccgtc gacgcgtgcg gtgacggtga cggtgtagaa agcctgcacg gtgccgagac
     2041 cctcgagata ctgcggcacg tcggcgcgcg cggcggtggc caggtccacc ggaatggcgg
     2101 gcgggggctt gggcaccgtg gcgggcctgt ggaagagcca gtacgccgcg agcagcacgc
     2161 acagcgcgac aaagccggcg atcgcttttt gcctcgctga tccgagcggc agcggcggga
     2221 gcttcacagg cgctcgtcct tctgctgcca gccgccgccg agcgcctgat agagcgacac
     2281 gagcgactgc agatgcgcgt acttggcctg cgcgagggcg tcctcggcgg tgaacaagct
     2341 ggtctccgtg ttgagaagag tcagcacgtt gatggtgccg gcgtgcatct gcgcctgggc
     2401 gaagcgatag gcacgccgcg cctgccccgt ggcctcctgc tgccggctca cctgttcggt
     2461 ggtctgctgc acggcgatga gggcgtcctc cacgttggcg agagccgaga tcaccgcctt
     2521 gtgatagtcg gcgagcagct cccggtactg cgccctggcg agcgagtagc cgccgagcaa
     2581 cgcgcccccc tcgaagaccg gctgggtgag gccggcgccg agcgaccaga tacggctcgc
     2641 gggagtgagg agcgtcgcga gcgccggact ctcgtagccg ccctcggcgg tgaggttgat
     2701 ggtggggaag atggcggcgc gcgccgcggc gatgtcggcg ttggcggcga tcagctgcgc
     2761 ttcggcggcg gcgacatccg ggcggcgggc gagcagccct gcgggcaatc ccggcttcac
     2821 cggcggctgg ctcaacgagt cgagtccccc gctcgtgacg tcgacggctt cgggtatcct
     2881 gccgatgagg atcgcgagcg catcgaggct ctgccgcagc tgctggcgca ggggcggaat
     2941 gccggcgttt accgtcgcga ccaccgtttc ctgctgcgca acgtcgagcg ccgtggcgat
     3001 gcccgcgctc tgctcgagct tcagcccgcg cagcacctgc gtggcgttgg cgaggttcga
     3061 gtcggcgatg ccgagccgat cgcgcagctc gagggcctga aagtaagtgg tggcgacgcc
     3121 tgccatgacg gtgagctcga ccgtggtgcg gtcgtagcgg ctggcgtgcg cgctggcggc
     3181 ggccgcggca cgcagcgcgc ggttcttgcc gaagaagtcg agctggtagc tcgcgacgag
     3241 cagcgggctg aagttgtcgg cgctcgcgta ggcgccattg ggcggaaccg cggcacgcgc
     3301 acgcgtggcc tgcgcgctta gcgcgagcgc tgggaggagc ggtgcgccgg cgatgcgcac
     3361 ctgcgcatcc gcctgctgca cccgcgccac ggccgcccgc agatcgtcgt tggcgccctg
     3421 cgcctcgctg atgagggtgt cgagctcggt gctgccgaat ccgcgccacc actcggtcgt
     3481 gggccactcg gataccgcgg catcgctcgc cgtcacccag tgatcgggag gcgcaatatc
     3541 gggcttgtgg tagcgcgggc cgaggctgca ggcggtgacg aggagcgcac tgcaccagag
     3601 gaggccggcg agcgaacgcg gacaggacat ccgcccgcgg atcttgcccc cacgaggcag
     3661 ccaggcacgc atgacgccac cttagccctg gcaaggttct ccctcagtgg gcgaatgatt
     3721 aaagtcggtt aatctctcga acgggggatg gcggccgggc ctgaagcgcg cattagatcg
     3781 gcgcccctgc gtggaggagc tgtcatgtcg atcaagctga ccgtgaacgg tgcggcgcgt
     3841 gagctggatg tggatcccga catgccgctg ctgtgggcga tccgcgacca cctcgagctg
     3901 agcgggacca agttcggttg cggaatggca ttgtgcggcg catgcacggt gcacatcgac
     3961 gggcagccga cgcgctcgtg cgttacgccg gtgtcggccg tcgccggcaa gagtgtgacc
     4021 actatcgagg gcctcgcgac accggccgcc aaggcggtgc agcaggcctg gatcgcgctc
     4081 gacgtgccgc aatgcggtta ctgccagagc gggcagatca tgtcggccgc ggccctgctc
     4141 ggcgcaaatc cacagccgag cgatgcggac atcgacgccg cgatggccgg caacatctgc
     4201 cgctgcgcca cttatgtgcg catccgcgcc gccattcatc aggccgccac ggcgctcgcg
     4261 gcgccgcgtc cgcaggggag ttgagccatg tacgtgcgct acctcgaaga ggccggtcgc
     4321 acccgcggcc cggccagctc actgacccgc cgccagttcc ttgagctcac gacactcgcc
     4381 ggctcgggtc tcacgcttgg catcctgctg ccgggctgcg gctcggccgg atcgggcggt
     4441 ggcgcggccg ccgcggcgac accgctcgag atgccgttcg tgcgcatcgc gccggacaac
     4501 accgtcaccg tactctgcaa gcac
//
LOCUS       DATLKL010000667         2002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_53023, whole genome shotgun sequence.
ACCESSION   DATLKL010000667 DATLKL010000000
VERSION     DATLKL010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53023"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..218
                     /locus_tag="VM713_09025"
     CDS             3..218
                     /locus_tag="VM713_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HVH84445.1"
                     /translation="MTCPHCGAKRIETMPTDACQFFYDCAGCGALLRPKPGDCCVFCS
                     YGTVPCPPRQASCASAARCGPPGETAA"
     gene            215..985
                     /locus_tag="VM713_09030"
     CDS             215..985
                     /locus_tag="VM713_09030"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylmercury lyase family protein"
                     /protein_id="HVH84446.1"
                     /translation="MTIEIRPGVERPDWSVVTSAAARQALLARDRRRLGSIETWPVGL
                     TASQDLVWRTTLELFARLGRPPQTVEIALEAKLAEAAVRQLVCELRSHDLLGLDDNAA
                     AIGYIYPLTARRTEHRITLNRHALHALCAVDALGVGAMYRADVVIESSCRSCGADITV
                     RTAQDGTALAAAQPAGVMVWYDLAYDRSAAASCCPSIAFFCGAQHLRQWRDAQGAKRV
                     GTPLTLDEAFEVGRALFGPVLRMSTEAYAAKGGRDRQI"
     gene            complement(1086..>2002)
                     /locus_tag="VM713_09035"
     CDS             complement(1086..>2002)
                     /locus_tag="VM713_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84447.1"
                     /translation="WGGRLTYGPELLLVSAVAAVGVLHTMVPDHWVPIALIARQRGWS
                     KGETARASLQAGAGHVLSTLLIALIIWLAGVAFARRFGSAVDSVSSLALIAFGGWIAI
                     SAWRDMRRHGGHGHAHSHDVPHIHGHEHGPGHGGHAHDHARGDDHGLVPADDPLYVPS
                     RGETAVLMQHIHLHRHGRSIHAHWHDHVPASFHAIAAETDSAPPAHEHRHKTTGRAAL
                     LLILGSSPMVEGIPAFFAAGKYGAGLIAVMASVFAISTIATYMFLCVYSAAGLQHMKF
                     GALERYGEVLSGGLITLVGLAFWIWPIL"
BASE COUNT          338 a          654 c          665 g          345 t
ORIGIN      
        1 cgatcacctg cccgcattgc ggagcaaagc ggatcgagac gatgccgacc gatgcctgcc
       61 aattcttcta tgattgtgcc ggctgtggcg cgctgctgcg ccccaagccg ggcgactgct
      121 gcgtgttctg ctcttacggc accgtgccgt gcccgccgag gcaggcatcc tgcgccagtg
      181 ccgcgcgctg cggcccgccg ggggagaccg cggcgtgacg atcgagatcc ggcccggggt
      241 cgaacgaccc gactggtcgg tcgtgacgag cgcggcggcg cggcaggcgc tgctcgcccg
      301 cgaccgccgc cggcttggct ccatcgagac ctggccggtc ggcctgacgg cgtcgcagga
      361 cttggtctgg cgcacgacgc tggagctgtt tgcccgactg ggccggccgc cacagacggt
      421 ggagatcgct ctggaggcga agctcgccga ggccgcggtc cggcagctcg tctgcgagct
      481 ccgatcgcat gaccttctcg gtttggacga caatgccgct gccattggct acatctatcc
      541 gttgacggct cggagaacgg aacaccgcat cacgctcaat cgccatgcgc tgcacgcgct
      601 atgcgccgtc gacgcgcttg gcgtcggggc gatgtaccgc gccgatgtcg taatcgagtc
      661 ctcatgtcgc agctgcggcg ccgacattac ggtccgaacc gcgcaagatg gaactgcgct
      721 cgccgccgct cagccggctg gcgtcatggt ctggtacgat ttggcctatg atcggagtgc
      781 tgcggcgtcg tgttgtccgt cgatcgcatt cttctgcggc gcgcagcacc ttcgccaatg
      841 gcgtgatgcg caaggcgcga agcgagtggg cactccgttg acgctcgacg aggcatttga
      901 ggtcggtcgg gcgctcttcg gcccggtcct tcgcatgtcc acggaggcct acgccgcgaa
      961 gggcggtcgc gatcggcaaa tatgagtcgc gaggttatca gcggcccagg atctcgaaaa
     1021 cgattgcttc ctgtccaggg gcgctgtggc gatcgcccgg ctgcattcgc tatgttccgg
     1081 tgtgactata ggatcggcca tatccagaat gccaatccga caagtgtgat caagccgccg
     1141 ctcaacacct cgccatagcg ttcgagcgcg ccgaatttca tgtgctgcag cccagcggcc
     1201 gaatacacgc ataagaacat gtaggtcgcg atcgtactga tggcgaatac ggaggccatg
     1261 accgctatga gcccggcgcc gtacttcccc gcggcgaaaa atgcgggaat gccttcgacc
     1321 attggcgagg agccaaggat cagcagcagc gctgcgcgcc cggtagtctt gtgacggtgc
     1381 tcgtgcgccg gcggggctga gtctgtctcg gccgcgatcg cgtggaacga ggcagggaca
     1441 tgatcgtgcc aatgcgcatg gatcgaacgg ccgtgccgat gaagatggat gtgttgcatc
     1501 aggacggccg tctcgccgcg cgatggcaca tagagagggt catcagccgg cacgagcccg
     1561 tggtcatctc cgcgcgcgtg atcgtgagcg tgcccgccat gaccagggcc atgctcgtgc
     1621 ccgtgaatgt ggggaacgtc gtggctgtga gcgtgcccgt gtccgccatg ccgccgcatg
     1681 tcccgccatg cggagatcgc aatccagccg ccgaaggcga tcagcgcaag gctcgacacc
     1741 gaatcgacgg cgctgccgaa gcgccgcgcg aacgccacac ccgccagcca gatgatcagg
     1801 gcaatcaaca acgtcgaaag gacgtgccct gcgccggctt ggagcgacgc ccgcgccgtt
     1861 tcgcccttcg accatccccg ctggcgcgca atcagcgcga tcggcaccca gtggtccggg
     1921 accatggtgt gcagcacgcc gacggccgcg acggcgctga caagcaaaag ctctggcccg
     1981 tatgtcaatc ttcctcccca tg
//
LOCUS       DATLKL010000668         3602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_19214, whole genome shotgun sequence.
ACCESSION   DATLKL010000668 DATLKL010000000
VERSION     DATLKL010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19214"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1124
                     /gene="lpxL"
                     /locus_tag="VM713_09040"
     CDS             201..1124
                     /gene="lpxL"
                     /locus_tag="VM713_09040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HVH84448.1"
                     /translation="MHWRLLAPRYWPTWVGIGVLRLLALLPYAAVIAVGALLGRLMRL
                     VAVRFATVARRNLELCFPELDPQARARLLDRHFASLGIALLEIPLAWWSSRQRIARLV
                     QVEGREHLEAAKARGRGVILLTAHFTPMEMAGRVLASVTPVGFLYRPTTNAVLAYALN
                     RFRAAHDGHPIARDDIRAFINALRNRECVWYAPDQSYRKKGAEMVRLFGIPAATNTLT
                     SRLARMTGAPVLPYFLQRLPGTQGYRAVIHAPLENFPTDDAVADTERFNHLIEAQVRL
                     APEQYLWIHRRFKGLTEDYPDYYGQEAPRRA"
     gene            1157..2620
                     /gene="glpD"
                     /locus_tag="VM713_09045"
                     /pseudo
     CDS             1157..2620
                     /gene="glpD"
                     /locus_tag="VM713_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
     gene            2742..3488
                     /locus_tag="VM713_09050"
     CDS             2742..3488
                     /locus_tag="VM713_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84449.1"
                     /translation="MSDSVLAAIIAGTATLSASFLQLRSAALRDAGKPGAAARRKNRL
                     QRLIFLVIVGGSAVSGFALSQWLTQGERVAQGALQKELQARIAEVSHTAGELEATRAA
                     ERSEIETSVLRRIGNDGVVVTATVPACRVARVSSVSAPASPTAVPAAVPPAARACGEA
                     EATPVTLCATIPAGATVTEVALFSRLDDADTPWSASRYLPGQEAGAARFADKPSETAP
                     EAGTRQVCQTFAHWDADHARVVRVLVRYSL"
BASE COUNT          500 a         1284 c         1222 g          596 t
ORIGIN      
        1 ccagcgctcg cctggacgga tgttccagct cagatccttc aacacccgcc gcccgttccg
       61 gcgcagctcg gcgtgcgcga ggcgcacttc gaggtagtgg ggatcggcgc cgctcatcgg
      121 ccgcattgga gcacagtttg ctcgggcgtt tggagtattc tgcgcgggcg caacgcgcgc
      181 gcaccttgtt ggagagctcg atgcattggc ggctgctcgc gccccgctat tggccgacct
      241 gggtcggcat cggcgtgctg cgtctgctgg cgctgttgcc ttatgcggcg gtgatcgccg
      301 tcggcgcact gctcggccgc ctgatgcgcc tcgtggcagt gcgctttgcg acggtcgccc
      361 ggcgcaacct cgagctgtgt tttcccgagc tcgacccgca agcgcgcgcg cggctcctcg
      421 accggcattt cgccagcctc gggatcgcgc tgctggagat tccgttggca tggtggagct
      481 cgcggcagcg catcgcccgc ctcgtgcagg tggagggccg cgagcatctc gaggcggcca
      541 aggcgcgcgg ccgtggcgtg attctcctca cggcgcattt caccccgatg gaaatggccg
      601 gccgggtgct cgcctcggtc accccggtcg gcttcctcta ccggccgacc accaacgcag
      661 tgttggctta cgcgctcaac cgctttcgcg ccgcccacga cggtcatccc atcgcgcgcg
      721 acgacatccg cgccttcatc aatgcgctgc gcaaccggga atgtgtctgg tacgcgcccg
      781 accagagcta ccgcaagaag ggggcggaga tggtgcgcct gttcggcata cccgcggcca
      841 ccaacacgct cacctcgcgt ctggcgcgca tgaccggagc gccggtgttg ccctactttc
      901 tgcagcgctt gccgggtacg cagggctatc gtgccgtgat ccatgcgccg ctcgagaact
      961 tcccgactga cgatgcggtg gcggacaccg agcgcttcaa tcacctgatc gaagcgcagg
     1021 tgcgcctcgc gcccgagcaa tatctgtgga tccaccgccg cttcaaggga ctcaccgagg
     1081 actacccgga ctactacggc caggaggcgc cgcgccgcgc gtgagtgagc ccggagacac
     1141 aagcttcgat ctgctggtgg tcggcggcgg gatcaacggc accgggatcg cgcgcgatgc
     1201 cgccgggcgc ggactgcgcg tgctgctcgt cgagcaggac gacctggcgg cccatacctc
     1261 ctccgccagc accaagctga tccacggcgg cctgcgctat ctcgaggagt tccacttcgc
     1321 cctggtgcgc aaggcgctca tcgagcgcga ggtgctgctg cgctcggcgc cgcacatcat
     1381 gcgaccgctc cacttcatca tgccgcatgc gccgcatctg cgtccggcgt ggatgatccg
     1441 cgcgggtctg ttcctctagg atcatctcgc gcgccgcgag cacctggccg cttccggcgc
     1501 ggtcgacctg cgcacgcaca tcgcgggcgg cgcgctcaag agcggctacc ggcgcggctt
     1561 cgtgtattcc gacggccaga ccgacgatgc gcgcctcgtg gtgctcaacg cgctcgacgc
     1621 ccgcgagcgc ggcgccgtga tcctgacgcg cacgcgctgc gagcgcctca ccgagcaggg
     1681 agggcgttgg ctcgcgagcc tcgccggtgc ggcggggagg accaccatcg aagcacgtgt
     1741 cgccgtgaac gcgagcggcc cctgggtggc ccggttcgtc gcggacgcga cgcccgcgcg
     1801 gaccgtgcat cgcgtgcggc tcgtgaaggg cagccacatc gtcgtgccgc ggctcttcga
     1861 ccatcgcttt gcctacattt tccagaacga ggatcggcgc atcgtgttcg ccatccccta
     1921 cgagcacgac ttcacgctgc tcggcacgac cgacctcgac tactacggcg atccggccgc
     1981 ggtgaggatc gggcccgagg aggtcgagta cctgtgcgag ctcgccaacc gttacttcgt
     2041 ccgcgccatc agcccgggtg aggtgctgtg gagctattcc ggcgtgcggc cgctgctcgc
     2101 cgacgagtca cgcgatccga tgagcgtgac gcgcgactac gcgctcgagc tcgagcggcg
     2161 tcccgctccg ctcctctcgg tgttcggcgg caagatcacc acctaccggc ggctcgccga
     2221 ggaagccgtg aacctgctga gccccctcct cggcacgcgc gccgagccct ggaccgcggg
     2281 cgcgaagctg cccggcggcg acgtgccgca gaagaggttc gcggtctttc tacgcacggt
     2341 cgagcggcgc tacccctggc ttcccccgcg gctgcagcag cgctacgcgc atgcctacgg
     2401 cacccgcatg gctcgcatgc tgggatcggc cggcgcgctc gcggacctcg gcgccgagct
     2461 cctccccggg ctctatgagc gcgaggtaga gtatctgcgt ctcgaggagt tcgcgcgcac
     2521 cgcggaggat atactctggc ggcggaccaa gctcgggctg catcttctcg ggaccgatcc
     2581 cacgccgctc gagcgctggc tcgcgacgca cggggcctga gcgggggcgt ggcgtgggca
     2641 cgacatgggc cgtgtgatca gcgtcgcggt ttttcactta agcccggcgc gcattgcgcc
     2701 acgcaggtag cgtggcggcg gagtcgcttg tgtttactca aatgtccgat tcagtcctcg
     2761 ccgctatcat cgcagggacc gcgacgctgt cggcgtcgtt cctgcagctg cgctcggccg
     2821 ccctgcgcga cgccggcaag cccggtgcgg ccgcgcgtcg caagaaccgc ctgcagcgcc
     2881 tcatcttcct ggtgatcgtc ggcggctcgg cggtgtcggg cttcgcgctc tcgcagtggc
     2941 tcacccaggg cgagcgcgtg gctcagggcg cactacagaa agagctgcag gcgcggattg
     3001 ccgaagtcag ccacaccgcc ggcgaactcg aggcgacgcg tgccgcggag cgctccgaaa
     3061 tcgagaccag cgtgctgcgc cggatcggca acgacggagt ggtggtcact gccaccgtgc
     3121 cggcgtgccg cgtggcgcgg gtgagcagtg tctccgcgcc cgctagtccg actgcggtgc
     3181 cggctgcagt gccgcctgcg gcgcgcgcct gcggcgaagc cgaggccact ccggtcacgc
     3241 tgtgcgccac gattcccgcc ggcgcaaccg tcaccgaggt cgcgctcttc agccgcctcg
     3301 acgacgcgga cacaccctgg agtgccagcc gctatctgcc cgggcaggaa gcaggcgcgg
     3361 cgcgctttgc cgacaagccc tccgagaccg cgcccgaggc cggcacccgt caggtgtgtc
     3421 agaccttcgc gcactgggac gccgaccacg cgcgcgtggt gcgggtgctc gtgcgctact
     3481 ctctctagcc cgacctacgc ataattgccc cttcaagagg cgtcagcctc cggggggact
     3541 gttggcttca tcaccgcgag agggcgcagc agcacgaggt actctgcttg tcgtcgtgct
     3601 tt
//
LOCUS       DATLKL010000669         3466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_143530, whole genome shotgun sequence.
ACCESSION   DATLKL010000669 DATLKL010000000
VERSION     DATLKL010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143530"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VM713_09055"
     CDS             <1..416
                     /locus_tag="VM713_09055"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HVH84450.1"
                     /translation="QLSHPWRSERVDRVAGIEARGFILGGAIAHQLSAGFVPIRKKGK
                     LPHATVRVAYSLEYGLDEMEMHRDAVGAGEDIILVDDLIATGGTAAGAVQLLRSLGAR
                     VLAGCFIVDLPDLGGSAKLTALGVPVQTLVAFPGH"
     gene            595..804
                     /locus_tag="VM713_09060"
     CDS             595..804
                     /locus_tag="VM713_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84451.1"
                     /translation="MFKKLIPVIAFALIAGPAFAQSTPAPAPAPAASSSSDTAKDTKA
                     THKKKHHKKTEKSGEKSDSSSTPPK"
     gene            972..1220
                     /locus_tag="VM713_09065"
     CDS             972..1220
                     /locus_tag="VM713_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84452.1"
                     /translation="MSVSHAVTSMTFPSCDQQDLIEAIDSVEEQLHTSHPNLALMCRF
                     LNSIARSLRAQPEARDACLLIEDAIGKAGMPSTWQSGI"
     gene            1256..1882
                     /locus_tag="VM713_09070"
     CDS             1256..1882
                     /locus_tag="VM713_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HVH84453.1"
                     /translation="MRHRERGSYERATVNAILDEALICHVGFVADGQPFVLPTTHARI
                     DEQLYLHGAATNHMLGSIADGGRLCVTVTLIDALVLARSYFRHSMNYRSVVVLGAGRQ
                     VTDPAEKGRALHALIERVHPGRSALIRPPTPQELEVTSVLALALEEASAKVRTGPPID
                     LETDYARPCWAGVLPLELAAGTPIPDARVPSGLAPPREILCWSRKPRG"
     gene            1955..>3466
                     /locus_tag="VM713_09075"
     CDS             1955..>3466
                     /locus_tag="VM713_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HVH84454.1"
                     /translation="MIAPLSQVLLLLAGSVFLVTLARRLGLPTALAYLVLGLLLGPHA
                     LGVVSDSGTTRLLAELGVAFLLFTLGLEFSLPRMLAMRGEVLGLGALQVGATGGVCAL
                     IAHGLGVGWLPAIVLGGAVSMSSTAILLQQLTERAELNRTHGRLAFSMLLFQDLAFVP
                     LLALAAALTEGADHFTLGESLVAVFGGALSIVAVLAAGRWLLRPLFHEIAHSRLRELF
                     TLTVLLVALGSAWVSHVAGLSFALGAFLSGMMLAETEYRHQIEAVIRPFRDILLGLFF
                     ISVGMLLDLHLLVRELGLVSLLLVIMVPLKAVIAALVARPFTTSRFKALRTGIVISIG
                     GEFGVALCTILLEAGLVPEKLGEPLVVAIVLSMVLSPVILNNNKRVARALLREHAPVA
                     TASEREEAATGGIARREHVILCGFGRVGQNVARVLESQGFEYIALDLDPARIRAARQA
                     GDPVMYGDSADEELLAKAGLSTASAVVISFSDPATSIGILRSLRRVRPDVPVLV"
BASE COUNT          495 a         1207 c         1157 g          607 t
ORIGIN      
        1 agcagctctc gcacccctgg cgcagcgagc gcgtcgaccg tgtagccgga atcgaggcgc
       61 gcggtttcat tctcggcggc gccatcgctc atcaactgtc ggcgggcttc gtgccgatcc
      121 gcaagaaagg caagctgccg cacgcgacgg tgcgcgtggc gtattcgctc gagtacggac
      181 tcgacgagat ggagatgcac cgcgatgcgg tgggcgccgg tgaggacatc atcctggtgg
      241 acgatctcat cgccaccggc ggcacagccg ctggggcggt gcagttgctg cgctcgctcg
      301 gcgcgcgcgt gcttgccggc tgcttcatcg tcgatctgcc cgatctcggc ggctcggcca
      361 agctcaccgc gctcggagta ccggtgcaga cactcgtcgc cttccccgga cactgagtga
      421 ggtttcttca gccgcggttc agataactcg cccttaaata tcaataagtt acatgcacat
      481 cgaacacaac attacgttgc ggtcatatgg ctcttgcccg gcgggaatcc tgtgataata
      541 cgcccgcctc gcgcacgcgg cgcacacata caaatccttc tggagaccac atcgatgttc
      601 aagaagctca ttcccgttat cgcgtttgcc ctgatcgccg gcccggcctt cgcccagtcg
      661 accccggctc cggccccggc tccggccgct tcgtccagct ccgacacggc gaaggacaca
      721 aaggccaccc ataagaagaa gcaccacaag aagactgaga agagcggcga gaagagcgat
      781 tcgtcctcga cgcccccgaa gtaaatattt cccgttgatc ttgaggggcc ccgccgcgca
      841 aacggcgggg ctccttgctt tctgggggac ggcggcatgg gatcggaccg cggggcttgc
      901 agcgcagccc aaaccggttc aaattctctc ccatggacgg ggagcagcag gccgcaatac
      961 atcaggcgct ggtgtccgtg tcacatgccg tgacgagcat gacgttcccg agctgcgatc
     1021 agcaggacct gatcgaagcc atcgatagtg tcgaggagca gctgcacacg agccacccga
     1081 atctcgcgct catgtgccgc ttcctcaact ccatcgcgcg cagcctgcgg gcgcagccgg
     1141 aggcgcgcga tgcgtgcctc ttgatcgagg atgccatcgg caaggccggc atgccgagca
     1201 cctggcagtc gggaatctga ccgagccgat tcgccccacg cctcgcacca cgctcgtgcg
     1261 ccatcgcgag cggggaagct acgagcgcgc gaccgtcaac gccatcctcg acgaggcgct
     1321 catctgccac gtcggcttcg tggccgacgg ccagccgttc gtgctgccga cgacccacgc
     1381 gcgcatcgac gaacagctct acctgcacgg cgccgcgacc aatcacatgc tgggctcgat
     1441 cgcggacggt gggcggctgt gcgtgaccgt cacgctgatc gacgcgctgg tgctggcgcg
     1501 ctcgtacttc cggcactcga tgaactaccg ctccgtggtc gtgctcggcg ccgggcgcca
     1561 ggtcaccgac ccggcggaaa agggccgcgc cctgcacgcc ctcatcgagc gggtgcatcc
     1621 ggggcgcagc gccctcatac ggccgccgac accgcaggaa ctcgaagtca ccagcgtgct
     1681 ggcgctggcg ctcgaagaag catccgccaa agtccgcacc ggcccgccga tcgacctcga
     1741 gacggactac gcgcggccct gctgggcggg tgtattgccc ctcgagctcg cagccggcac
     1801 gccgatcccc gatgcgcggg tcccgtccgg gctcgcgccg ccgcgagaga tcctttgctg
     1861 gtcgcgtaag ccgcgcggtt gagccgggcg cgtccggcgc tccgccgcgc tcccggttgc
     1921 gttcgccgcg cggcgcagtc taagttggga ggcgatgatc gcgccgctct cccaggtcct
     1981 gctgctgctc gccggctccg ttttcctggt gacgctcgcg cggcgtctcg gcctgccgac
     2041 cgccctcgcc tatctcgtcc tcggactcct gctcggcccg catgcactcg gggtggtgtc
     2101 ggactcgggc acgacgcggc tgctggccga gctcggcgtg gcgtttctgc tcttcaccct
     2161 cgggctcgag ttctcgctgc cgcgcatgct ggcgatgcgc ggcgaggtgc tgggcctcgg
     2221 agcgctgcag gtcggagcca ccggcggcgt gtgcgcgctc attgcccacg ggctcggcgt
     2281 cggctggctg ccggcgatcg tgctgggggg cgcggtatcg atgagctcga ccgcgatcct
     2341 gctgcagcag ctcaccgagc gcgccgagct caaccgcacg cacggccggc tcgccttcag
     2401 catgctgctg ttccaggacc tggcgttcgt gccgttgctg gcgctcgcgg cggcgctcac
     2461 cgagggcgcc gaccatttca cgctcggcga gagtctggtc gcagtgttcg gcggcgcgct
     2521 gtcgatcgtg gcggtgctgg ccgccgggcg ctggttgctg cgcccgctgt ttcacgagat
     2581 cgcccacagc cggctgcgcg agctcttcac cctcacggta ctgctggtgg cgctcggctc
     2641 ggcctgggtg tcgcacgtcg cgggactgtc gttcgcgctc ggcgccttcc tctcgggcat
     2701 gatgctcgcc gagacggagt accggcacca gatcgaggcg gtcatccgtc cgttccgtga
     2761 catcctgctc ggcctgttct tcatctcggt cggcatgctg ctcgatctgc acctgctggt
     2821 gcgcgagctg gggctggtgt cgctgctgct ggtgatcatg gtgccgctca aagcggtgat
     2881 cgcggcgctg gtcgcgcgcc ccttcacgac ctcgcgtttc aaggcgctgc gcaccggcat
     2941 cgtgatctcg atcggcggcg agttcggagt ggcgctgtgc acgatcctgc tcgaggcggg
     3001 gctggtgcca gagaagctcg gcgagccgct cgtggtcgcg atcgtgctgt cgatggtgct
     3061 ttctccggtg atcctcaaca acaacaagcg cgtggcccgc gcgctgctgc gcgagcatgc
     3121 tcccgtggcc accgccagcg agcgcgagga ggccgccacc ggcgggatcg cccggcgcga
     3181 gcacgtgatc ctctgtggct tcggccgcgt gggccagaac gtagcgcgcg tgctggagtc
     3241 gcagggcttc gagtacatcg cgctcgatct cgatccggcg cgcatccgcg ccgcgcgcca
     3301 ggcgggggat ccggtcatgt acggcgactc tgccgacgag gagctgctcg ccaaggcggg
     3361 cctgagcacc gccagtgcgg tggtcatcag cttctccgat ccggccacct cgatcggcat
     3421 cctgcggagc ctgcgccgtg tacgccccga cgtgccggtg ctggtg
//
LOCUS       DATLKL010000670         2162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_199679, whole genome shotgun sequence.
ACCESSION   DATLKL010000670 DATLKL010000000
VERSION     DATLKL010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199679"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1241)
                     /locus_tag="VM713_09080"
     CDS             complement(57..1241)
                     /locus_tag="VM713_09080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVH84455.1"
                     /translation="MRVLFISDVYFPRVNGVSTSIRTFRQDLAAHGVETQLVAPRYEA
                     ASGAAAEEPGVLRVASAGVPRDPEDRRMRWGALTRALDGLTASGFDLVHIHTPFIAHY
                     AGVRFARRAGIPAVATYHTFFEEYLHHYLPVLPHGLGRWLARSFTRSQCDDVRALIAP
                     SEPMRAVLLEYGVATPIHVLPTGLPADRFRPGDGSAFRARAGLAPDAPLVTYIGRVAH
                     EKNIGFLVQMFREVLARVPRAMLVIAGEGPARTALQQQVASLGLARHVHFAGYLERDT
                     VLLDCYAAADVFVFASRTETQGLVLLEAMAQGAPVVSTAELGTRSILVPGSGALVVAE
                     EPHAFAAAVVRVLENPQLREELAQRGRVYARSWSSSAMAARLTELYETLRSAPGAALG
                     AA"
     gene            complement(1366..1905)
                     /locus_tag="VM713_09085"
     CDS             complement(1366..1905)
                     /locus_tag="VM713_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVH84456.1"
                     /translation="MKASSLNAFMARIDAAEYGLCRTLNRGAAFAAPRLVFRVASRLG
                     DGIIWYLLIFLLPVLYGMAALRPAIVMAFTGVLGVTIYTLLKRVFVRERPFITHAAID
                     LKGAPLDRYSFPSGHTLHAVSFAWQATAHFPELGWVLVPLAALIAASRVVLGLHYPSD
                     VLAGAAIGAALAELGTSLA"
BASE COUNT          383 a          713 c          772 g          294 t
ORIGIN      
        1 ctcgtgcacg gggtaagggc agaccgcggc caacacgtcg tttcccgaac aagctctcag
       61 gctgcaccca gcgccgcgcc cggcgcgctg cgcagcgtct cgtacagctc ggtaagccgc
      121 gcggccatgg cggaggagga ccagctgcgc gcgtagacgc gtccgcgctg cgcaagctcc
      181 tcgcgcagct gtggattctc gagcacgcgc acgaccgccg cggcaaacgc gtggggctcc
      241 tcggcaacca ccagcgcgcc gcttcccgga acgagaatgg accgcgtgcc aagctcggcg
      301 gtcgagacga cgggcgcccc ctgcgccatt gcctcgagca gcaccagccc ttgcgtctcg
      361 gtgcgcgagg cgaacacgaa tacgtccgcg gccgcgtagc agtcgagcag taccgtgtcg
      421 cgttcgaggt agccggcgaa gtgcacgtgc cgagcgaggc cgaggctcgc cacctgctgc
      481 tgcagtgccg tgcgcgccgg gccctccccg gcgatcacca gcatggcccg cggtacccgc
      541 gccaggacct cgcggaacat ctgtaccaga aagccgatgt tcttctcgtg tgccacgcgc
      601 ccgatgtagg taacgagcgg cgcatcgggc gcgagccccg cgcgggcacg gaaggcgctg
      661 ccatccccgg gacggaaacg gtcggccgga agcccggtgg gcagcacgtg aatcggcgtt
      721 gcgaccccgt actcgagcag cacggcgcgc atcggctcgg acggcgcgat cagggcgcgg
      781 acgtcatcgc actgcgagcg cgtgaagctg cgggcgagcc agcgtccgag gccgtgcggc
      841 agcaccggca ggtagtggtg cagatactcc tcgaagaagg tgtggtaggt cgccacggcc
      901 gggatgccgg cgcggcgcgc gaagcgcacg ccagcatagt gggcgatgaa gggagtgtgg
      961 atgtgcacca ggtcgaagcc cgaggccgtg agcccatcga gcgcccgcgt gagagcgccc
     1021 cagcgcatac ggcggtcctc gggatcgcgc ggcacccccg ctgaagccac acgcagcaca
     1081 cctggctctt cggccgccgc ccccgatgcg gcctcatagc gcggcgcgac gagctgtgtc
     1141 tcgacgccgt gggcggccag atcctggcgg aaagtgcgga tggaggtgga gacgccgttg
     1201 acgcgcggaa agtagacgtc cgagatgaag aggacgcgca agcgctagct gactggaaaa
     1261 atcgcctgcg gcgatttttc ggccttcgtt gccgggcgcc acaggcgcga ctttggcgcc
     1321 ctgcgcttcg caggggccgt ggggatgcga cgcatccgcg agattctaag ccagggacgt
     1381 gccgagttcc gcgagcgcgg cgccgatcgc cgcccccgcg agcacatcgg acggatagtg
     1441 caggcccagc accacgcgcg aagcggcaat cagggctgcg agcggcacga gcacccagcc
     1501 gagctcgggg aagtgcgcgg tcgcctgcca ggcgaaggac acggcgtgca gggtatggcc
     1561 cgaggggaag ctgtagcggt cgagaggcgc gcccttgaga tcgatcgcgg catgggtgat
     1621 gaaggggcgc tcgcgcacga acacgcgctt caggagcgtg tagatcgtga cgcccagcac
     1681 cccggtgaac gccatcacga tcgccggccg gagggccgcc attccgtaga gcaccggcag
     1741 aaggaagatg agcaggtacc agatgatccc gtcgccgagg cggctcgcga cccggaacac
     1801 gagccttggc gccgcaaagg cagccccccg attcagcgtc cggcataggc cgtactccgc
     1861 cgcatcgatc cgggccatga aggcgttgag gctggacgct ttcatacgcg ctacagcctc
     1921 gcgctgcagc gcgacagcgc cattacaaat atgtgaaagt tttgttgcta caaggggtta
     1981 cttgctcttt tgtgtgtgct gtctcacata tttgacagat tacttccagc tcggggagcg
     2041 tcgccaaggc gtgcgagcag cgcggcggcg aagcgctcgg ccgaccctgg cgcgtgggag
     2101 aggaaaagcg acggttccgt cagctcgagc tcgagaagcc gcggcgcctg gtgcgcatcc
     2161 cg
//
LOCUS       DATLKL010000671         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_87053, whole genome shotgun sequence.
ACCESSION   DATLKL010000671 DATLKL010000000
VERSION     DATLKL010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87053"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VM713_09090"
     CDS             <1..527
                     /locus_tag="VM713_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVH84457.1"
                     /translation="TRAALEAALAPGAIQILVNNTGVHDDAVLPGMNPAQWRRVIDVS
                     VNGFFNATQPLLMPMIRARWGRIVSVTSVAALIGNKGQVNYSAAKGALNSATRALALE
                     VATRGVTVNAVAPGIIATPMTDGLFDAAAIERLVPMNRLGSPEEVAGVVAFLASEDAA
                     YVTGQVLSVSGGMA"
     gene            573..1709
                     /locus_tag="VM713_09095"
     CDS             573..1709
                     /locus_tag="VM713_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HVH84458.1"
                     /translation="MSNHARLALCGLIVLATVPAGIRAAATDTPAAPVSAAQLIESLG
                     LHVAPTPVRERSDWHPPRVILVGSLLQDLHGLLPRLRETAPAVRFVAIDQATPRDIAA
                     ADATIGVCSAEVLKAARHLAWIQWPAAGVDRCVQQPLLRERAPLLTNLQRTMGASIAE
                     HVIALMLALSRHFEDFSREQAQAHWEPYDSAPELEDVGGKTVLIAGLGGIGTEVARRA
                     HALGMRVVATRASGHEGPDFVSYVGLPDELPKLAKDADFVVNCLPLTPETTGIFNKEF
                     LAAMKRSAYFISIGRGRSTVTADLAAALTQGTIAGAGLDVVDPEPLPPESPLWHAPHL
                     IITPHISANTAVAEQTRNAVLVENLRRYVAGEPMLSVVDIAKGY"
     gene            complement(1713..>2137)
                     /locus_tag="VM713_09100"
     CDS             complement(1713..>2137)
                     /locus_tag="VM713_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84459.1"
                     /translation="RLLTGGADQRAVEDFVRMERWIFDSPPQAAVALTQFVRWLYQEN
                     RLVRGTLELGGRAVDLKSVRQPLLNLFARADHIVPPAASEVLGRYVGSRDYTACDIDT
                     GHIGMYVSRKARAEIPQRIISWLEARSGGNARRPRGRT"
BASE COUNT          339 a          769 c          706 g          323 t
ORIGIN      
        1 ccacacgcgc agctctcgag gccgccctgg cgcccggtgc catccagatt ctcgtcaaca
       61 acaccggtgt gcatgacgat gcggtgctcc cggggatgaa tcccgcgcag tggcggcggg
      121 tgatcgacgt atcggtcaac ggctttttca atgcgacgca gccgctcctc atgcccatga
      181 tccgcgcgcg ctggggccgg atcgtgagcg tgacctccgt cgcggcgctg atcggcaaca
      241 aggggcaggt caattactcc gccgccaagg gtgcgctcaa ctccgcgacc cgcgcactcg
      301 cgctcgaagt cgcaacacgc ggcgtcacgg taaacgccgt agccccgggg atcatcgcga
      361 ctcccatgac ggatggcctg ttcgatgccg cggccatcga gcgtctggtg ccgatgaatc
      421 gcctgggttc gcccgaggag gtggcgggcg tcgtcgcctt tctcgcctcg gaggacgccg
      481 cctacgtgac gggccaggtg ctctcggtga gcggcggcat ggcgtagact cgcgcataac
      541 aataacgact gccctgctcc ggagcccgcc ctatgtcgaa ccacgcccga cttgctctct
      601 gcgggttaat cgttctggcc acggtccccg cggggatccg cgccgcggcg accgacaccc
      661 cggcggcgcc cgtctccgcg gcgcagctga tcgagagcct cgggctgcac gtagcgccca
      721 ccccagtacg cgagaggagc gactggcacc cgccacgcgt gatcctcgtg ggcagccttc
      781 tgcaagacct gcacggcctg ctgccgcgcc tcagggagac ggcgccggca gtacggttcg
      841 tcgcgatcga tcaggcgacg ccgcgtgaca tcgccgcggc cgatgccacc atcggcgtgt
      901 gctctgccga ggtgctgaag gcggctcggc atctcgcctg gatccagtgg ccggctgcgg
      961 gcgtcgaccg ctgcgtgcag cagcccctgc tccgggagcg ggcgccactg ctcaccaacc
     1021 tgcagcgcac gatgggagcg agcatcgccg agcacgtcat cgcgctgatg ctggccctga
     1081 gccgtcactt cgaggacttc tcccgcgagc aagctcaagc tcactgggag ccctatgact
     1141 cggcgccgga gctcgaggac gtcggcggca agaccgtgct catcgccgga ctcggcggga
     1201 tcggcaccga ggtggcgcgg cgcgcccacg ccctaggcat gcgcgtcgtc gcgacgcgcg
     1261 cgagcggcca cgagggaccg gacttcgtga gctacgtcgg gctccccgac gagctgccga
     1321 agctcgcaaa ggacgcggac ttcgtcgtca actgcctgcc actgactccc gagacgaccg
     1381 gcatcttcaa caaggaattc ctcgccgcca tgaagcgttc cgcctacttc atcagcatcg
     1441 ggcgcggacg cagcacggtg accgccgatc tcgccgcagc gctcactcag ggcaccatcg
     1501 cgggcgcggg gctcgatgtc gtcgatcccg agccgctgcc gcccgagagt cccttgtggc
     1561 acgcgccgca tctcatcatc acgccgcaca tctcggccaa cacagcggtc gcagagcaga
     1621 cccgcaatgc ggtgctcgtc gagaacctgc gccgctatgt ggccggcgag cccatgctct
     1681 cggtggtcga catcgcgaag ggctactgag cctcaggtac gcccccgcgg ccggcgggcg
     1741 ttgccaccgg agcgcgcctc gagccaggag atgatccgct gcggaatctc cgcgcgcgcc
     1801 ttgcggctca cgtacatccc aatgtgtccg gtgtcgatgt cgcacgcggt gtaatcgcga
     1861 ctgccaacgt aacgccccag cacctcggag gcggccggcg gcacgatgtg gtcggctctg
     1921 gcaaagagat tgagaagggg ctggcgcacc gatttgaggt ccaccgcccg gccgccgagc
     1981 tcgagcgtgc cacgcaccag gcggttctcc tggtagagcc agcgcacgaa ctgggtcagc
     2041 gccaccgcgg cctgcggcgg gctgtcgaag atccagcgct ccatgcgcac gaagtcctcc
     2101 accgcgcgct gatcggcgcc gcccgtgagc agccgca
//
LOCUS       DATLKL010000672         1862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_120965, whole genome shotgun sequence.
ACCESSION   DATLKL010000672 DATLKL010000000
VERSION     DATLKL010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120965"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1862
                     /locus_tag="VM713_09105"
     CDS             <1..>1862
                     /locus_tag="VM713_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH84460.1"
                     /translation="ALLGIGEAARMPVDIFPKMDIPVCSVVWNYAGMSATDIQNRILT
                     LHERQMASLVDDIARIEATSYEGVGVEKVYLHEGADVTRAISQLASSALVVLKYMPRN
                     ITPPLVIRYGATDVPIIQLSLSSSSLPDTALNDLGQNIIRPALAVVHGAEVPYPYGGK
                     PRVIMADLDQQALQARALTPADVSAALQAQNVILPAGDVKIGKLDYLVAMNNSPDVIE
                     AINSFPIKRVDGRQVFMRDVAHVHDGFQVQTNSVSVDGVPGALMTIRNTGGVSTLAVI
                     AGLRAALPDIRHMLPGDVSIKPIFDQSVFVKAALNSVALGGTMAAGLTALMILLFLGN
                     WRLTTMILAAIPLSIITAVLLMGAGGETLNTMTLGGFALAVGILVDNGTVVIENIERH
                     AGLHEHLRDAIVHGAGEVGLPTFLSTLCICIVFVPVFLLTGTAKYLFSPLSVAVIVSL
                     LASLALSFTMVPVMFLYLMRAQHQRERASTAVAAARPQVARLLQLLRNAHVTFEGAFH
                     RFREVYRNALAWALSQPKATVLAFLAIMIASLPLFPLLGRDFFPEVDAPQMRLHVRAP
                     PGTRLETTQQDFARVEAAIRALVGNRQVRVILDNIGTPYSGINTALSDSATVGP"
BASE COUNT          292 a          657 c          599 g          314 t
ORIGIN      
        1 cgcgctgctc gggatcggcg aggcggcgcg catgccggtc gacatcttcc cgaagatgga
       61 cataccggtg tgcagcgtcg tgtggaacta cgccggcatg agcgccaccg acatccagaa
      121 ccgcattctc acgctgcacg agcggcagat ggcctcgctg gtcgacgaca tcgcgcgcat
      181 cgaggccacc agctacgagg gcgtcggcgt cgaaaaggtc tacctgcacg agggcgcgga
      241 cgtgacgcgc gccatctcgc agctcgcgag cagcgctctg gtcgtgctca aatacatgcc
      301 gcgcaacatc acgccgccgc tggtcatccg ctacggcgcg accgacgtac cgatcattca
      361 gctcagcctc tcgagcagct cgctccccga cacggcgctc aacgacctcg gccagaacat
      421 catccgcccg gcgctcgcgg tggtgcacgg cgccgaggtg ccgtatccct acggcggcaa
      481 gccgcgcgtc atcatggccg acctcgacca gcaggccttg caggcgcggg ctctgacccc
      541 ggcggatgtg agcgcggcgc tgcaggcgca gaacgtcatc ctgccggcgg gcgatgtgaa
      601 gatcggcaaa ctcgactacc tcgtggcgat gaacaacagc ccggacgtga tcgaggcgat
      661 caactccttc cccatcaagc gcgtcgacgg gcgccaggtc ttcatgcgcg acgtcgccca
      721 cgtgcatgac ggcttccagg tgcagaccaa ctcggtgtcg gtcgacggtg tgccgggcgc
      781 gctcatgacg atccgcaaca ccggtggggt ctcgacgctc gcggtgatcg cggggctccg
      841 cgccgcgctt cccgacatcc gccacatgtt gcccggcgac gtgagcatca agccgatctt
      901 cgaccagtcg gtgttcgtca aggccgcgct caacagcgtg gctctcggcg gcaccatggc
      961 cgctgggctc acggcgctca tgatcctgct gtttctcggc aactggcgcc tcacgaccat
     1021 gatcctcgcg gcgatcccgc tgtcgatcat tacggcggtg ctgctgatgg gagcaggcgg
     1081 ggagaccctc aataccatga cgctcggcgg cttcgcgctc gccgtcggga tactggtgga
     1141 caacggcacg gtggtgatcg agaacatcga gcggcacgcg gggttgcacg agcacctgcg
     1201 ggacgccatc gtccacggcg cgggcgaggt cgggctgccg acgttcctct cgacgctgtg
     1261 catctgcatc gtgttcgtgc cggtgttcct gctgaccggc acggcgaagt acctgttctc
     1321 gccactgtcg gtggcggtga tcgtctcgct gctggcgagc ctcgcgctgt cgttcaccat
     1381 ggtacccgtc atgttcctgt acctgatgcg cgcgcagcat cagcgcgagc gcgcctcgac
     1441 cgcagtggca gcggcgcgcc cgcaggttgc gcgcctgctg cagctgctcc gcaacgcgca
     1501 cgtgaccttc gagggcgcct ttcaccgctt ccgcgaggtg taccgcaacg cgctcgcctg
     1561 ggcgctgtcg cagccgaagg ccacggtgct ggcatttctc gccatcatga tcgcctcgct
     1621 gccgcttttt ccgctgctcg gtcgcgactt cttccccgag gttgacgccc cacagatgcg
     1681 cctgcacgtg cgcgccccgc ccggcacgcg cctcgagacc acgcagcagg acttcgcgcg
     1741 ggtcgaagcg gcgatccgcg cacttgtcgg caaccgtcag gtgagggtga tccttgacaa
     1801 catcggcacg ccctacagcg gcatcaacac cgcgctcagc gattcggcca cggtgggccc
     1861 ca
//
LOCUS       DATLKL010000673         1701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_53109, whole genome shotgun sequence.
ACCESSION   DATLKL010000673 DATLKL010000000
VERSION     DATLKL010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53109"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VM713_09110"
     CDS             complement(<1..259)
                     /locus_tag="VM713_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVH84461.1"
                     /translation="MSVSVSMPQLGESVTEGTVTRWLKREGERVEADEPLLEVSTDKV
                     DTEIPSPVSGILRGITVAEDETAAVGAELAVIDEEGAEDGAA"
     gene            complement(276..1655)
                     /gene="lpdA"
                     /locus_tag="VM713_09115"
     CDS             complement(276..1655)
                     /gene="lpdA"
                     /locus_tag="VM713_09115"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HVH84462.1"
                     /translation="MADRGGYDIVILGGGSGGYACALRAAELGKRVALIEKGKVGGTC
                     LHRGCIPTKALLHAAEVADQARESATFGVRATYVGIDMPGVNAYKEKVVTRLWRGLVG
                     LIGSRHIDIIDGEGRLVSPTQVQVGDSLIEGAHVVLASGSEPRSLPGLDIDGQRVITS
                     DQALELDRVPASVVILGGGVIGVEFASVWRSFGAEVTIVEMLPHLLPPEDEASSKLLE
                     RAFRRRGIAYKLGARFEGVKENGSGVTVTLEGGATIDAELLLVAVGRGPVSAGLGYED
                     VGVAMQRGYVTVDEYCQTSVPTISAVGDLIPGPQLAHVGFAEGIMVAERLAGLPVTPI
                     DYDGVPRVTYSNPEVASVGITSAMAAERGIKTREITYDLAGNGRSQILQTSGAAKVIA
                     AEDGGPVLGIHLVGARVGELIAEGQLIYNWEALPSEVAQLIHPHPTQSEIIGEAHLAL
                     AGKPLHFHS"
BASE COUNT          228 a          628 c          573 g          272 t
ORIGIN      
        1 gggccgcgcc gtcctcggct ccctcctcgt cgatgaccgc gagctcggcg ccgaccgcag
       61 ccgtctcgtc ctcggcgacc gtgatgccgc gcaggatgcc cgacaccggc gaggggatct
      121 cggtgtcgac cttgtccgtg gacacctcga gcagcggctc gtcggcctcg acccgctccc
      181 cctcccgctt cagccagcgc gtgactgtgc cttctgtgac gctctcgccg agctgtggca
      241 tggatacgga gaccgacatg acttcccgcg actccttacg agtggaagtg gagaggtttg
      301 cccgcgagcg ccaggtgcgc ctcgccgatt atctccgact gggtggggtg cggatggatg
      361 agctgggcga cctccgacgg cagggcctcc cagttgtaga tcagctggcc ctcggcgatc
      421 agctcgccga ctcgcgcgcc gaccaggtgg atgccgagca ccgggccgcc gtcctcggcc
      481 gcgatgacct tggcggcgcc cgaggtctgc aagatctggc tcctgccgtt gccggccagg
      541 tcgtaggtga tctccctggt cttgatgccc cgctcggcgg ccatcgcgga ggtgatgccc
      601 accgacgcga cctcggggtt gctgtaggtc acccgcggca ccccgtcgta gtcgatgggc
      661 gtgaccggca gcccggccag ccgctcggcg accatgattc cttcggcgaa cccgacgtgc
      721 gccagctgcg ggcccgggat caggtcgccg accgcggaga tcgtgggcac gctcgtctgg
      781 cagtactcgt ccaccgtgac gtagccgcgc tgcatggcca ccccgacgtc ctcgtagccg
      841 agaccggccg agaccgggcc gcgcccgacg gcgacgagta gcagctcggc gtcgatcgtg
      901 gcgccgccct ccagtgtgac cgtgacgccg gaaccgttct ccttcacgcc ctcgaacctg
      961 gcgcccagct tgtaggcgat gccgcgacgc cggaacgcgc gctcgagcag cttcgagctg
     1021 gcctcgtcct ccggcggcag caggtgcggc agcatctcga cgatcgtgac ctcggcgccg
     1081 aacgagcgcc acacgctcgc gaactcgacg ccgatcacgc cgccgccgag tatgaccacc
     1141 gacgcgggca cccggtccag ctcgagcgcc tggtcgctgg tgatcacccg ctggccgtcg
     1201 atgtcgagcc cgggcaggct gcgtggctcg gagccgctgg ccagcacgac gtgcgcgccc
     1261 tcgatcagcg agtcgccgac ctgcacctga gtgggcgaca cgaggcgccc ctcgccgtcg
     1321 atgatgtcga tgtgccggct gccgatcagg ccgacgagcc cccgccagag ccgggtgacc
     1381 accttctcct tgtaggcgtt gacgccgggc atgtcgatgc cgacgtaggt ggcccgcacg
     1441 ccgaaggtag cgctttccct ggcctggtcg gcgacctccg cggcgtgcag cagcgccttg
     1501 gtcgggatgc atccccggtg caggcaggtg ccgccgacct tgcccttctc gatgagcgcc
     1561 acgcgcttgc cgagctcggc ggcgcgcagc gcgcaggcgt agccgccgct gcctccgccg
     1621 aggatcacaa tgtcgtagcc gccgcggtct gccacggtga actctccttc gctcagcgct
     1681 atctacctag aggcgtccgt c
//
LOCUS       DATLKL010000674         3337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_7860, whole genome shotgun sequence.
ACCESSION   DATLKL010000674 DATLKL010000000
VERSION     DATLKL010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7860"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..686
                     /locus_tag="VM713_09120"
     CDS             63..686
                     /locus_tag="VM713_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVH84463.1"
                     /translation="MTSTRGRGRPRHDDVLTPTEWRVVHAIQHGMSNREIAARRGISL
                     DAVKYHVANAVAKLGVANRQGLRRWFRVPRGSALAGKEQPMTNLNLGPLAQIARTVRD
                     IKESEAWYGKTLGLTHLYSFGSFAFFDCGGTRLYLSATDKPGPESILYLRVADIRSAY
                     DTLRARGVEFGQAPHMIHRHADGTEEWLAHFNDPEGRPLGILAQVRP"
     gene            complement(737..1813)
                     /locus_tag="VM713_09125"
     CDS             complement(737..1813)
                     /locus_tag="VM713_09125"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HVH84464.1"
                     /translation="MTPRRVALLLACGLVLIAFAIWLSSRRHLEHATLAGDLVLPGLE
                     QSVNTVSEVDLRRGDGTHASLKKASTGWVVGERDWAAEPGKVRKLLLDLGALNVVEEK
                     TRLPANYPQLGVEDVSTPKATGTQIDVLTPARKFALIVGKTSSGKSGYVRVAGNAASL
                     LAAPLLTPDADPKTWLERSLLDLPATRVREVDQKPADGPAFSAARDKKEQADFSVAPI
                     PKGRLLTSAGAADSIAASVRGLTLEDVQKASAPSDAKAAHAVFRTFDGLELEVAGRKD
                     GARALITVAARATAKDAEAEAHALNTRLGGWEFEIPDYKYGAIFRTLEELLQPKPVKP
                     APHKPTTKAGAKAPADSGAKPSEK"
     gene            complement(1810..>3337)
                     /locus_tag="VM713_09130"
     CDS             complement(1810..>3337)
                     /locus_tag="VM713_09130"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HVH84465.1"
                     /translation="PVGATGEKLYFGLAGTNSIDGHAAIEFFDPNKEQFLEYDVVRLV
                     YQLANPKKPVLAWLSTLPMGPGFDPQTGQMREPWVIYGQAEQLFDIRPLEPTATKIDP
                     EVNVLVLVHPKNLSPALQFAIDQYALKGGHILAFVDPMSEADSSGNDPRNPMASMGAD
                     KSSHLGELLTAWDVKFDATQVVADRGHALSVSMHQGEPPVEHLGVLGLDKSSFAADDV
                     ITAGLSNVNVAVAGYLEPIKGASVKFEPLLESSTDAALLGVERFRMLFDPASLHEGFK
                     PSGVRYVLGARVTGDVRTAFPGGAPAGVTLPAGATALKASVHPLSLVVIADTDLLADY
                     LWVHQQEFFGQRLVQAWASNGGLVLNALDNLAGSDDLISVRGRATFTRPFERVERLRR
                     AADERYRDKEHELEQQLQATEQKLTALQSKGGKSESSLIVTPQEQEEIEHFGQEKIRI
                     RKELRAVRAGLDADISRLGTEIKIVDIIVIPAAFALLALGFAAWRRRARSHGRAEQRP
                     "
BASE COUNT          490 a         1118 c         1176 g          553 t
ORIGIN      
        1 accggtacac gggggctttg ggggagggta aatcagtatc cgaggcctca ggatctgccg
       61 gtatgacgag cacgcgaggc agagggcgcc cccggcacga tgacgttctg acgcccaccg
      121 aatggcgggt ggtgcacgct atccagcacg ggatgagcaa ccgcgagatt gccgcgcgcc
      181 gcggcatcag tctcgatgcg gtcaagtatc acgtggcgaa cgccgtggcg aagctcgggg
      241 tcgcgaaccg ccaggggctg cgccggtggt tccgcgtgcc tcggggcagc gcgctcgcgg
      301 gcaaggagca accaatgacg aacctgaatc tggggccgct ggcgcagatc gcgcgcaccg
      361 tgcgcgacat aaaggagtcg gaagcctggt atggcaagac gctcggcctc acgcacctct
      421 acagcttcgg ctcgttcgcg ttcttcgact gcggcggcac gcggctgtat ctgagcgcga
      481 ccgataagcc cggcccggag tcgatccttt acctgcgcgt cgcggacatc cgctcggcct
      541 acgacacgct gcgcgcccgc ggagtggagt ttggccaggc gccgcacatg attcatcgcc
      601 atgccgacgg caccgaggag tggctggcgc acttcaacga tccggagggc cgcccgctcg
      661 gcatcctcgc acaggtcagg ccgtgaggcg gagccgccgc gcgtcccaaa ctagcgggtg
      721 aattcctggt ggggcgttac ttctcggagg gcttggcgcc gctgtctgcc ggcgccttcg
      781 cgcccgcctt cgttgtcggc ttgtgcggcg cgggcttcac gggcttgggc tgcagcagct
      841 cctcgagtgt gcggaagatc gcgccgtact tgtagtcggg aatctcgaac tcccagccgc
      901 cgagccgcgt gttgagcgcg tgcgcctcgg cctcggcgtc cttggccgtc gcgcgcgccg
      961 cgaccgtgat gagcgcacgc gcgccgtcct tgcggccggc gacttcgagc tcgaggccgt
     1021 cgaaggtgcg gaagacggcg tgcgcggcct tggcgtcgct tggcgcggac gccttttgca
     1081 cgtcctcgag cgtgagcccc ctcacggacg ccgctatcga gtccgcggcg ccggcgctcg
     1141 tcaggaggcg ccccttaggt atgggcgcca cgctgaagtc ggcctgctcc ttcttgtcgc
     1201 gcgcggcgct gaaggctggt ccgtcggcgg gcttctggtc cacctcgcgc acgcgcgttg
     1261 cgggcagatc gagcagtgat cgctcgagcc aggtcttggg atccgcatcg ggggtgagga
     1321 gcggcgccgc gagcagactc gcggcgttgc ccgcgacgcg cacgtagccc gacttgccgc
     1381 tggaggtctt gccgacgatg agcgcgaact tgcgggccgg ggtgagcacg tcgatctgcg
     1441 tgccggtcgc tttcggcgta ctgacatcct cgacgccgag ctgcggatag ttcgccggca
     1501 ggcgcgtctt ctcctcgacc acgttcagcg cgccgagatc gagcaggagc ttgcgcacct
     1561 tgccgggctc ggcggcccag tcgcgctcgc cgaccaccca acccgtcgag gccttcttga
     1621 ggctcgcgtg tgtgccgtca ccgcggcgca ggtctacctc gctcacggtg ttgacgctct
     1681 gctcgaggcc cggcagcacc aggtcgccgg cgagcgtcgc gtgctcgagg tgccgccgcg
     1741 acgacagcca gatcgcgaac gcgatcagca ccagcccgca cgcgagcagc agcgcgacgc
     1801 ggcgcggcgt catggccgct gctccgcgcg cccgtggctg cgtgcgcggc gccgccaggc
     1861 cgcaaatccg agcgccagca acgcgaatgc cgccgggatc acgatgatgt ccacgatctt
     1921 gatctcggtg ccgagccgcg agatgtcggc atcgagcccg gcgcgcacgg cgcgcagctc
     1981 cttgcggatg cggattttct cctgcccgaa gtgctcgatc tcttcctgct cctgcggcgt
     2041 gacgatgagc gagctctcac tcttgccacc cttggattgc agcgcggtca gcttctgttc
     2101 ggtcgcctgc agctgctgct cgagctcgtg ctccttgtcg cggtagcgct cgtccgcggc
     2161 gcgccgcagc ctctcgacgc gctcgaaggg gcgggtgaag gtggcgcgac cgcgcacgct
     2221 gatcaggtcg tcgctgccgg cgagattgtc gagggcgttg agcaccagac cgccgttgct
     2281 ggcccaggcc tgcaccagac gctggccgaa gaactcctgc tggtgcaccc acagataatc
     2341 ggccagcagg tcggtatcgg cgatcaccac gaggctgagc ggatggaccg aagccttgag
     2401 cgccgtcgcg cccgcgggca gcgtcacgcc ggcgggcgcc ccgcccggaa aggccgtgcg
     2461 tacgtcgccg gtaactcgtg cgccgaggac gtagcgcacg ccgctcggct tgaagccctc
     2521 gtgcagcgag gccggatcga acagcatgcg gaagcgctcg acccccagca gggccgcatc
     2581 agtgctcgac tcgagcagcg gctcgaactt gacgctcgcc cccttgatcg gctcgaggta
     2641 gccggcgacc gcgacgttga cgttggagag tcccgcggtg atcacgtcgt cggcggcgaa
     2701 gctcgacttg tcgagcccca gcacgccgag atgctcgacc gggggctcac cctggtgcat
     2761 ggagacggag agtgcgtggc cgcggtcggc cacgacctgg gtggcgtcga acttgacgtc
     2821 ccaggcggtg aggagctctc cgaggtgaga ggacttgtcg gcgcccatcg acgccatcgg
     2881 gttgcggggg tcgttccccg aggagtcggc ctcggacatg ggatcaacga aggcgaggat
     2941 gtggccacct ttgagcgcgt actggtcgat cgcgaactgc agcgccggcg acagattctt
     3001 cggatgcacc agcaccagca cgttcacctc ggggtcgatc ttggtggcgg tcggctcgag
     3061 cggccgtatg tcgaagagct gctccgcctg cccgtagatc acccacggct cgcgcatctg
     3121 gccggtctgc gggtcgaagc ccggacccat cggcagcgtc gacagccagg cgagcacggg
     3181 cttcttcgga ttggcgagtt gatagacgag ccgcaccacg tcgtactcga ggaactgctc
     3241 cttgttggga tcgaagaact cgatggccgc gtggccgtcg atggagttgg ttccggcgag
     3301 cccgaaatag agtttctccc cggtcgctcc cacgggt
//
LOCUS       DATLKL010000675         2221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_19276, whole genome shotgun sequence.
ACCESSION   DATLKL010000675 DATLKL010000000
VERSION     DATLKL010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19276"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..694
                     /gene="rpsC"
                     /locus_tag="VM713_09135"
                     /pseudo
     CDS             1..694
                     /gene="rpsC"
                     /locus_tag="VM713_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388013.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            699..1103
                     /gene="rplP"
                     /locus_tag="VM713_09140"
     CDS             699..1103
                     /gene="rplP"
                     /locus_tag="VM713_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HVH84466.1"
                     /translation="MLQPKRTKYRKQFKGRNGGFAQRGAHVSFGEFGLKATSRARMTA
                     REIEAARRAMARYVKRGGQIWIRVFPDVPISKKPLEVRMGSGKGNVEYYVARVQPGKV
                     LFEMEGVDEPTAREAFRLAGSKLSVSTMFVKR"
     gene            1113..1346
                     /gene="rpmC"
                     /locus_tag="VM713_09145"
     CDS             1113..1346
                     /gene="rpmC"
                     /locus_tag="VM713_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HVH84467.1"
                     /translation="MKLAQVRQFREKLRSHSEADLADELLALRKEQFALRMQRATGQQ
                     PKPDQFSAARRNIARVKTALRQKQLAGGGGKGK"
     gene            1544..1798
                     /gene="rpsQ"
                     /locus_tag="VM713_09150"
     CDS             1544..1798
                     /gene="rpsQ"
                     /locus_tag="VM713_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HVH84468.1"
                     /translation="MRGARTLTGRVVSNKMQKTIAVEIERLVKHPTYGKYIRRTTKLL
                     AHDENGSSQEGDLVIITPCRPMSRHKSWRLLEIVAKAARS"
     gene            1795..2163
                     /gene="rplN"
                     /locus_tag="VM713_09155"
     CDS             1795..2163
                     /gene="rplN"
                     /locus_tag="VM713_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HVH84469.1"
                     /translation="MIQLRTMLNAADNSGARTLMCIKVLGGTRRRYATVGDVIKVSVK
                     DAIPRGKVKKGEVYDAVVVRTTHGVRRPDGSLIRFDGNAAVLLTNKLEPIGTRIFGPV
                     TRELRNVQFMKIISLAPEVL"
BASE COUNT          424 a          743 c          744 g          310 t
ORIGIN      
        1 actttcgcgt gcgcgagttc ctccatgaga agctcaagga ggcgtccgtc agccgcatcc
       61 agatcgagcg cgccgccaag aaggtcaaca tcaccatcca gaccgcgcgt cccggcatcg
      121 tgatcggcaa gaagggcgaa gacatcgaga agctgcgcca ggagaccgcc gcgctcctcg
      181 gcatgagcgt gaacgacgta cgcctcaata tcgccgagat cagaaagccc gagctcgatg
      241 cgcagctggt cgcggactcc atcgcgcagc agatcgagaa gcgcgtcttg ttccgccgcg
      301 ccatgaagcg cgcggtgatg agcaccatgc gctcgggcgc cctcggggtg aaggtgcgcg
      361 tctccggcag actcaacggc tcggagatcg cgcgcacgga gtggtaccgc gagggccgca
      421 ttccgctgca caccttccgc gcggacatcg actacggcct cgcgcaggcg cgcaccacct
      481 acggcgtgat cggcgtgaag gtctggatct tcaagggcga ggtcttcgac aagagcgagc
      541 tcgccgcgca agaaccggcc gaggaggctg cgcccgccac tgctgcacct gccgctgcga
      601 ccccggccgt ggccgcgccc gtggccccag ccgctgcgcc tgcggccagc gcgtccgccg
      661 agccgccggc ccaggcctga tcgtgaaggc gtaacgagat gctgcagccc aaacgcacca
      721 agtaccgcaa gcagttcaaa ggccgcaacg gcggctttgc gcagcgcggc gctcacgtgt
      781 ccttcggcga gttcggcctc aaggccacca gccgtgcgcg catgacggca cgcgagatcg
      841 aggcggcgcg ccgcgccatg gcgcgctacg tgaagcgcgg tgggcagatc tggatccgcg
      901 tctttccgga tgtaccgatc tcgaagaagc cgctcgaggt ccgcatgggc tccggcaagg
      961 gcaacgtcga gtactacgtg gcgcgcgtgc agcccggcaa ggtgctgttc gagatggaag
     1021 gggtggatga gccgaccgcg cgcgaggcgt ttcgcctcgc cggctcgaag ctctcggtgt
     1081 ccaccatgtt cgtgaaacgg taggttcgct agatgaaact cgctcaggta cgtcagtttc
     1141 gcgagaagct gcgcagccac tcggaggccg acctcgccga cgagctcctg gcactgcgca
     1201 aggagcagtt cgcgctgcgc atgcagcgcg ccacgggcca gcagcccaag cccgatcagt
     1261 tcagtgccgc gcgccgcaac atcgcgcgcg tgaagacggc gctgcgccag aagcagctcg
     1321 ccggcggcgg aggtaaaggg aaatgagccc gacgaaggcc aaaaccagca aggcgacggc
     1381 cgcaaaatcg aagaccacta aggcgaagtc gcacgccaag gcgaaggcgg cagcaccggc
     1441 tgcggcgccc aaggcgacga cgcccccgcc cgctcccgcg gctgccttgg ccaccgttga
     1501 gactgccgct gctgagactg gggctgccca ggctgccccg gcgctgcgcg gtgcacgcac
     1561 cctcaccggg cgcgtcgtga gcaacaaaat gcagaagacc atcgcggtcg agatcgagcg
     1621 gctggtgaag cacccgacct acggcaagta catccgccgg accacgaagc tcctggcgca
     1681 cgatgagaac ggcagctcgc aggagggcga tctggtcatc atcacgccct gccgtcccat
     1741 gtcgcggcac aagtcatggc ggctgctcga gatcgtcgcg aaggcggcac ggtcatgatc
     1801 cagctgcgca ccatgctgaa tgccgctgac aacagcggcg cgcgcaccct gatgtgcatc
     1861 aaggtgctgg gcggtacccg gcgccgttat gcgaccgtgg gcgatgtcat caaggtgagc
     1921 gtcaaggacg cgattccgcg cggcaaggtg aagaagggcg aggtctacga tgcggtcgtg
     1981 gtgcgcacca cgcacggcgt gcgccgtccc gacggctccc ttatccgctt cgacggcaat
     2041 gcagcggtgc tgctcaccaa caagctcgag ccgatcggca cgcgcatctt cgggcccgtg
     2101 acgcgtgagc tgcgcaacgt gcagttcatg aagatcatct cgctggcccc cgaggtcctc
     2161 tgaactcatg aacaagatcc gcaagggcga ccaggtggtg gtgctttccg ggcgcgacaa
     2221 g
//
LOCUS       DATLKL010000676         1629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_177248, whole genome shotgun sequence.
ACCESSION   DATLKL010000676 DATLKL010000000
VERSION     DATLKL010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177248"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VM713_09160"
     CDS             complement(<1..968)
                     /locus_tag="VM713_09160"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HVH84470.1"
                     /translation="MSAHDVKSAERPAPDAALTTIAEYARNFRVTSQGAYETARYCLM
                     DTLACGFLALKYPACRKLLGPVVPGAVMPGGARVPGTSYELDPVQAAFNIGAMIRWLD
                     FNDTWLAAEWGHPSDNLGGILAVADYRARLAVMSGEAPPTVRDVLTAMIKAHEIQGVL
                     ALENSFNRVGLDHVLLVRVASTAVVTAMLGGSLEQVVNAVSNAWIDGGALRTYRHAPN
                     TGSRKSWAAGDATSRAVRLALIALTGEMGYPSALSVKTWGFCDVLFKGKPLSLPRPFG
                     SYVMENVLFKISYPAEFHAQTAVEAAMTLHPAVRDRIADIERIVIET"
     gene            complement(1013..>1629)
                     /locus_tag="VM713_09165"
     CDS             complement(1013..>1629)
                     /locus_tag="VM713_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HVH84471.1"
                     /translation="WVRAMHTSLILYAEHEFNASTFAARVIAGTGSDLYSCIDGAIGA
                     LRGPKHGGANETALVIQQRYESVAQAEADIRARVANKEVIMGFGHPVYTIADPRNEVI
                     KEVARRLSKAAGDLRRFEIAERIEAVMRETKNMFANLDWFSAVAYHMMGIPTEMFTPL
                     FVIARTTGWSAHVIEQRADGKIIRPAANYVGPENLKFVPIEKRK"
BASE COUNT          243 a          539 c          577 g          270 t
ORIGIN      
        1 gtctcgatga cgatgcgctc gatgtcggcg atgcggtcgc gcaccgccgg gtggagcgtc
       61 atcgccgcct cgaccgcggt ctgcgcgtgg aactccgccg ggtaggagat cttgaagagc
      121 acgttctcca tcacgtacga gccgaagggc cgcggcagcg agagcggctt gcccttgaac
      181 agcacgtcgc agaagcccca ggtctttacc gagagcgccg acggatagcc catctcgccc
      241 gtgagcgcga tgagggcgag gcgcaccgcg cggctcgtgg catcgcccgc ggcccagctc
      301 ttcctcgagc cggtgttggg ggcgtggcgg taggtgcgca gcgccccgcc gtcgatccag
      361 gcattggaga ccgcgttcac gacctgctcg aggctcccgc cgagcatcgc cgtcaccacg
      421 gcggtggacg ccacgcgcac cagcagcacg tggtcgagcc cgacgcggtt gaagctgttc
      481 tcgagcgcca gcacgccctg gatctcgtgt gccttgatca tggcggtgag cacgtcgcgc
      541 accgtgggcg gcgcctcgcc cgacatcacc gcgagccggg cgcggtagtc ggccacggcg
      601 aggatgccgc cgaggttgtc ggaggggtgc ccccactcgg cggcgagcca ggtgtcgttg
      661 aaatcgagcc agcggatcat ggcgccgatg ttgaaggccg cctgcaccgg gtcgagctcg
      721 tacgaggtgc ccggcacacg cgccccgccc ggcatcaccg ctcccggcac gacgggaccg
      781 aggagcttgc ggcacgccgg gtatttcagc gccaggaagc cgcaggcgag cgtgtccatc
      841 aggcagtagc gggccgtttc gtaggcgccc tggctggtca cgcggaagtt tcgcgcgtat
      901 tcggcgatgg tcgtgagcgc ggcatcgggg gccgggcgct cggcggattt cacatcgtgg
      961 gcggacatag actcttgtgg gcgcgcctgc gggacgacgg cgccgctcgt ggttacttgc
     1021 gcttctcgat cggcacgaac ttcaggttct cgggaccgac atagttggcg gccggccgga
     1081 tgatcttgcc gtcggcgcgc tgctcgatga catgcgccga ccagccggtg gtgcgggcga
     1141 tgacgaacag cggcgtgaac atctcggtgg ggatgcccat catgtggtag gcgaccgccg
     1201 aaaaccagtc gaggttggcg aacatgttct tggtctcgcg catcaccgcc tcgatgcgct
     1261 cggcgatctc gaagcgccgc agatcgcccg ccgccttcga cagccgccgc gcgacttcct
     1321 tgatcacctc gttgcgcgga tcggcgatcg tgtataccgg gtgtccgaag cccatgatga
     1381 cttccttgtt cgcgacgcgc gcgcggatgt cggcctcggc ttgggctacg gactcgtagc
     1441 gctgctggat cacgagcgcc gtctcgttcg cgccgccgtg cttaggcccc cgcagcgcgc
     1501 cgatcgcgcc gtcgatgcac gagtagagat ccgagcccgt gccggcgatg acgcgcgctg
     1561 cgaaagtcga ggcgttgaac tcgtgctcgg cgtacaggat cagcgaggtg tgcatcgcgc
     1621 gcacccact
//
LOCUS       DATLKL010000677         3312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_30698, whole genome shotgun sequence.
ACCESSION   DATLKL010000677 DATLKL010000000
VERSION     DATLKL010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30698"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VM713_09170"
     CDS             complement(<1..382)
                     /locus_tag="VM713_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVH84472.1"
                     /translation="MTKAIFGNHSSVLVPRRERDQIRRFYCDVLGGQLTKADPDRDFV
                     RLGEDFYIAFLYGDVADASEFLRSARSVWLEIKSDDVEEMIRKILAFGVRRLEVPDPH
                     LYFQAPGGQCLRLVGIDEDLSFYEG"
     gene            complement(402..836)
                     /locus_tag="VM713_09175"
     CDS             complement(402..836)
                     /locus_tag="VM713_09175"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVH84473.1"
                     /translation="MARSTFVYVTYIRTTPERLWSALTDAGFMRQYWFGMRGESEWTS
                     GSPWKLVSSEGQIMDAGEIVAAEPPRRLVIRWQNQWKPELKAEGYSLCTMELEPSGTA
                     VKLSVTHSIERDASKFIEAVSGGWPKVLSNLKSLLETGSAGL"
     gene            complement(829..1161)
                     /locus_tag="VM713_09180"
     CDS             complement(829..1161)
                     /locus_tag="VM713_09180"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVH84474.1"
                     /translation="MAERHDTDLLFRALADPSRRKLLDLLHAHDGRTLTELCEHLDMT
                     RQGVTQHLDLLEAANLLATLRRGREKLHFLNPVPLQEIYERWIAKFEKPRLKALSDLK
                     RRLEKTNG"
     gene            1660..2838
                     /locus_tag="VM713_09185"
     CDS             1660..2838
                     /locus_tag="VM713_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84475.1"
                     /translation="MRAHQAANYPRETVNRSIPEVREAHSTGGARMRAMSHGPAGNEV
                     RVVRYGNGVNGTVERSIRPGLVSRTFVGGGHVLYSRVYQQQVWHQFGRAFTYETFVPA
                     VRYPAVYYSWALGPWPRPLIYTWGWQAQPWYPAYGILFTPYPVYTSPDLWMTDYVIAR
                     SLQTAYQAQTPAPVVEPQPPQAPPPPAADDGALAPPVPPVDSSPPPSEVAPAPPAGDA
                     PAAPTAAPPAITPRIKAQLNEQIKVQLREQQAAAATPATLTDQNTPPALRPNHVFFEV
                     VHPLEVPADTAKGFCSLSTNDYIKRVGGMSEDDWKVPVVVELSGPSDCPQGLRTRIGL
                     NDLNAMENEQEARVMEAMQAASPSLGPNGPPRGAHPTLIADGSAAPDPMALDAIRQAR
                     "
     gene            2882..>3312
                     /locus_tag="VM713_09190"
     CDS             2882..>3312
                     /locus_tag="VM713_09190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVH84476.1"
                     /translation="MIGELIDHYRVLELIGRGAMGVVYKALDVNLDRQVAIKVMNAEA
                     RDDPDFVERFRQEARMQGALNHPNVAMLFDYFVHDGAPVAVMELIEGESLEQLIRRRG
                     AIPVRESIPIFKQALRGVAAAHRAGIIHRDLKPSNLMVTKDG"
BASE COUNT          570 a         1118 c         1069 g          555 t
ORIGIN      
        1 ctccttcata aaaggagagg tcctcgtcga ttccgaccag ccgcaggcac tggccgcccg
       61 gagcctggaa gtaaaggtgt ggatccggta cctcgagcct cctcactccg aaggcgagga
      121 ttttccggat catctcctcg acgtcgtcgg acttgatctc gagccacaca gacctggcgc
      181 tgcgcagaaa ctcgctcgcg tcagcgacat cgccgtagag gaaggcgata tagaaatcct
      241 cgcccaggcg cacgaagtca cggtcgggat cggctttcgt aagctgcccg ccgaggacgt
      301 cacaatagaa tcggcgaatc tgatcgcgct cccggcgcgg caccagaacc gacgaatggt
      361 tgccaaaaat tgccttggtc atggtcattc ctttgggggt cttacagacc ggcggagccg
      421 gtctcgagga gcgacttgag gttcgagagg accttcggcc agccgccgga caccgcctcg
      481 atgaatttcg aagcgtcgcg ctcgatgctg tgggtgacgg agagcttgac cgcggtgcca
      541 cttggttcca gttccatcgt gcagagtgaa tagccttcgg ccttgagctc gggcttccac
      601 tggttctgcc agcggatcac caggcgccgc gggggctcgg ccgcgacgat ctcgccggcg
      661 tccatgatct gaccctcgct ggacaccagc ttccacgggg acccggaagt ccactcgctc
      721 tcgccgcgca tgccgaacca gtactgcctc atgaaccccg cgtcggtgag cgctgaccac
      781 agtctctccg gcgtggttcg gatgtaggtc acgtagacaa aggtcgatct agccattggt
      841 tttctccagt cgtcgtttca ggtcgctcag cgccttgagg cgcggctttt cgaacttggc
      901 aatccagcgc tcgtagatct cctgcagcgg cacgggattg aggaagtgca gcttctcgcg
      961 gccgcgccgc agtgtggcga gcaggttcgc cgcctccagc aggtcgaggt gctgcgtcac
     1021 gccctgccgc gtcatgtcga ggtgctcgca cagctcggta agggtgcggc cgtcgtgcgc
     1081 gtgcagcagg tcgaggagct tgcgccggct cgggtcggcc agggctctga acagcaggtc
     1141 ggtgtcgtgg cgctctgcca tgtgttatac gcaagtgatt acttgcctaa atgataggca
     1201 agtatggcct tgcctgtcaa gcgcgccagt tccacgattc atggccgctg caaccgaggc
     1261 tcacagcgac atccaggatg ccgcgaggag tgtgctgcgg ttctcaaaag ctccgcggct
     1321 gcggcggttc cgcgacgcag ttcgcaccgc gctgtactcc gcgcgctaca gttaagcgct
     1381 acctccttcc agagtggtgc aaccgtggcc cgcggcgtct ggagtgacaa aatgtgcaga
     1441 ctggatcgta gggcctgcgc gctgctcgcc tggctggccc tcgcgagcct tgccggccct
     1501 gccccggtcg atgcgcgggg cgcggagcca gtgcatgagc agcggccctc cgagagagcg
     1561 cggcaggctc accgcgaagc cggtgcacga ccgagccacc cgaatcatgc ggcgcgcgtc
     1621 cagggcgccc atcccgccgc cggccgcgag gtctctcccg tgcgcgcgca ccaggccgcg
     1681 aactatcccc gggaaacggt caatcgcagc atccccgaag tgcgcgaggc gcattccacc
     1741 ggcggcgcgc ggatgcgcgc aatgtcccac ggccccgccg gaaacgaggt ccgcgtcgtc
     1801 cgctacggca acggtgtcaa cggcacggtc gagcgcagca ttcggccggg tctcgtgagc
     1861 cgcacgttcg tcggtggcgg acacgtgctc tattcgcgcg tctaccaaca acaggtctgg
     1921 caccagttcg gccgggcgtt cacctacgag accttcgtgc cggcggtgcg ctatcccgcg
     1981 gtctactact cctgggcgct cggcccctgg cctcgtccgc tcatctatac ctggggttgg
     2041 caggcgcagc cgtggtaccc ggcgtacggc atcctgttca cgccgtaccc ggtgtacacc
     2101 agcccggatc tgtggatgac cgactacgtc atcgcgcgga gtctccagac tgcctatcag
     2161 gcccagactc ccgcgccggt cgtggagccg caaccacctc aggctccgcc gccgcctgct
     2221 gcggatgacg gcgcgctagc gccgcctgtc ccgcccgtcg actcatcgcc gccaccgagc
     2281 gaggtagcgc cggcgccgcc cgcgggcgat gcaccggcag cgccgaccgc cgcgccacct
     2341 gccatcacgc cgcgaatcaa ggcgcagctc aacgagcaga tcaaagtgca gctgcgggag
     2401 cagcaagccg ctgccgcgac cccggcgaca ctcacggatc aaaacacgcc gccggcgctc
     2461 cggcccaatc acgtgttctt cgaggtcgtc catccgctcg aggttcctgc ggacaccgcc
     2521 aagggcttct gctcgctgag caccaacgac tacatcaagc gggtcggcgg aatgagcgag
     2581 gacgattgga aggtgccggt ggtggttgag ctgagcggac catccgactg cccgcagggc
     2641 ctcaggacgc gcatcggact caacgacctc aacgccatgg agaacgaaca ggaagcccgg
     2701 gtgatggagg cgatgcaagc ggcctccccg agcctcgggc cgaatggacc gccgcgcggc
     2761 gcgcatccga cattgattgc cgacggcagc gccgctccgg atccgatggc gctggacgca
     2821 atccgacagg ctcggtgagc aaggtgcgcg gctgctcgcc ggcgagtccc tgggagcgag
     2881 catgatcggc gagctcatcg accattaccg cgtcctcgag ctcatcggcc gcggcgcgat
     2941 gggcgtcgtc tacaaggcgc tcgatgtgaa tctcgaccgc caggtcgcca tcaaggtgat
     3001 gaacgcggag gcccgcgacg accctgactt cgtcgagcgc ttccgtcagg aagcgcgcat
     3061 gcaaggcgcc ctcaatcacc cgaatgtcgc gatgctcttc gactacttcg tccacgacgg
     3121 cgcccccgtc gcagtgatgg agctcatcga gggcgagagc ctggagcagt tgatccggcg
     3181 gcgcggggcg atccccgtcc gcgagagcat cccgatcttc aaacaggcgt tgcgcggggt
     3241 ggcggcggcg catcgcgccg gcatcattca tcgtgatctc aagccgagca acctgatggt
     3301 caccaaggac gg
//
LOCUS       DATLKL010000678         1500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_75993, whole genome shotgun sequence.
ACCESSION   DATLKL010000678 DATLKL010000000
VERSION     DATLKL010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75993"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1374)
                     /gene="dnaA"
                     /locus_tag="VM713_09195"
     CDS             complement(37..1374)
                     /gene="dnaA"
                     /locus_tag="VM713_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HVH84477.1"
                     /translation="MEASLWTRCMGALEAELPEQQFNTWVRPLQAMEGNGALKLLAPN
                     RFVVDWVNANLLPRIGELLRDESTGDVPIVTVEVGSRSQTLRGALPPALAPPRSRPAE
                     GLVVGARLNADFTFSSFVEGKSNQLAKAAALQVAENPGCAYNPLFLYGGVGLGKTHLM
                     HAIAHLIKENDAEARIAYVHSERFVSDMVRALQHNSMNEFKMAYRSLNALLIDDIQFF
                     AGKERSQEEFFHTFNELLEGQHQVILTCDRYPKEVEGLEERLKSRFGWGLTVAIEPPE
                     LETCVAILMTKARAAGVQLPEEVAFFIAKRIRSNVRELEGALRRVMANSRFTGRAITV
                     EFTKEALKDLLSLQAKLVTIENIQKTVADYYKVRMADLLSKRRSRSVARPRQVAMALA
                     KELTSHSLPEIGDAFGGRDHTTVLHACKRIKELRDTEQRMKEDYSNLLRTLAG"
BASE COUNT          247 a          487 c          493 g          273 t
ORIGIN      
        1 caagttcgtc cacaggaaat gcacagacga acaacctcaa ccggccagcg ttctcaacag
       61 gtttgagtag tcttccttca tgcgctgctc tgtatcgcgc agttccttga tgcgcttgca
      121 ggcgtgcagc accgtggtgt gatcgcgccc gccgaaggca tcgccgatct ccggcaggct
      181 gtgactggtg agctccttgg caagcgccat ggcaacctgc cgtgggcgcg cgaccgagcg
      241 gctgcgccgt ttggacagca ggtccgccat gcgcaccttg tagtagtcgg cgacggtctt
      301 ctggatgttc tcgatcgtca ccagcttcgc ctgcagcgac agcaggtcct tgagcgcttc
      361 cttggtgaat tccaccgtga tcgcgcggcc cgtgaagcgc gagttggcca tcacgcggcg
      421 cagtgcccct tcgagctcgc gtacgttgga gcggatgcgc ttggcgatga agaacgccac
      481 ttcctcgggc agctgtacgc cggcggcgcg cgccttggtc atgaggatcg ccacgcaggt
      541 ctcgagctcc ggcggctcga tggccaccgt gaggccccag ccgaagcgcg acttcaagcg
      601 ctcctcgagg ccttcgacct ccttcggata gcggtcgcag gtgaggatca cctggtgctg
      661 cccctcgagc agctcgttga aggtgtggaa gaactcctcc tgcgagcgct ccttgccggc
      721 gaagaactgg atgtcatcga tcagcagcgc gttcagcgac cggtaggcca tcttgaactc
      781 gttcatcgag ttgtgctgca gcgcgcgcac catgtcgctg acgaaacgct ccgagtgcac
      841 ataggcgatg cgcgcctcgg catcgttctc cttgatgaga tgcgcgatcg cgtgcatgag
      901 atgggtcttg ccgagtccca caccgccgta gaggaacagc gggttgtagg cgcagccagg
      961 attctctgcg acctggagcg cggcggcctt ggcgagctgg ttgctcttgc cctcgacgaa
     1021 ggacgagaag gtgaagtcgg cattcaggcg tgcgccgacc acgagtccct cggccggccg
     1081 cgagcgcggg ggtgcgagcg ccggcggcag cgcgccacgc agcgtctggc tgcgcgagcc
     1141 gacctcgacc gtcacgatgg gcacgtcccc ggtgctctca tcgcgcagca gctcgccgat
     1201 gcgcggcagg aggttggcat tcacccagtc gacgacgaag cgattcggcg cgagcagctt
     1261 cagcgcaccg ttgccctcca tcgcctgcaa cggccgcacc caggtgttga attgctgctc
     1321 gggaagctcc gcctccaacg cgcccatgca gcgagtccaa agcgacgctt ccacgcgcga
     1381 ttgcctccgc gtcttgaact ggggtctgtg aagaggaaaa ggccgcgaag tctataccga
     1441 tcggcgcgcg cgctgaaggc ttcgcggcgc gtgcgccggg agtgctgcgc cgcgcgcgct
//
LOCUS       DATLKL010000679         1642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_53153, whole genome shotgun sequence.
ACCESSION   DATLKL010000679 DATLKL010000000
VERSION     DATLKL010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53153"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VM713_09200"
     CDS             complement(<1..685)
                     /locus_tag="VM713_09200"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HVH84478.1"
                     /translation="MRLSYQPARVRRVSNPKDFGRVAVMLGGDSSEREISLLTGNAVL
                     EALKRRGVDAHAFDPREQPLTALTRKRFARVWIALHGPGGEDGTLQGALEYLGIPYTG
                     SGVMGSAIGMDKLRTKRLALSCGVPTADFVVLRGAQDFELALERLKLPLIVKPATQGS
                     SVGMSKVDRAEDLPAAFAAAAALETLVFAEPWITGGEYTVSVLQGAALPSIRIETPRT
                     FYDYEAKYFR"
     gene            complement(689..>1642)
                     /gene="murB"
                     /locus_tag="VM713_09205"
     CDS             complement(689..>1642)
                     /gene="murB"
                     /locus_tag="VM713_09205"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HVH84479.1"
                     /translation="RHGAAPARGARGAAAAGRRVVTVAVAAGFSTRVRRGEPLSRHTS
                     WHVGGPAEVFFNPRDRTELAAFLRGLPEGVPICWIGLGSNLLVRDGGLPGVVISTHGT
                     LDSLERLSETQVRAGAGVACARLARQCIKWGLGPAEFFAGIPGTLGGALAMNAGAFGG
                     ETWQHVLSLETIDRHGKERMRDASEYQVGYRTVDAPAPEEWFISATLGFEHRPGAHAD
                     EVRSLLARRKESQPIGEWSCGSVFTNPPGDHAARLIEAAGLKGFRMGDASVSQKHANF
                     IINHGTARAADLERLIEHVRSTVERVHGVSLHPEVRIVGVP"
BASE COUNT          241 a          595 c          580 g          226 t
ORIGIN      
        1 cgcggaagta tttggcctcg tagtcgtaga aggtgcgcgg cgtctcgatg cgaatcgagg
       61 ggagcgctgc gccctgcagg accgacacgg tgtactcgcc acccgtgatc cagggctcgg
      121 cgaacaccag cgtctcgagg gcggccgcgg ccgcgaaggc ggcgggcaag tcctcggcac
      181 ggtccacctt gctcatgccg acactcgagc cctgggtcgc cggcttcacg atgagcggca
      241 gcttcagccg ctcgagcgcg agctcgaagt cctgcgcccc gcgcagcacc acgaagtccg
      301 ctgtgggcac cccgcacgag agcgccaggc gcttggtgcg cagcttatcc atgccgatgg
      361 ccgagcccat taccccgctc ccggtgtagg gaatgccgag gtactcgagc gcgccctgca
      421 gcgtaccgtc ctcgcccccg ggcccgtgca gcgcgatcca cacccgcgcg aagcgcttgc
      481 gcgtgagcgc cgtaagcggc tgctcgcgcg gatcgaaggc atgggcgtcg acgccgcggc
      541 gcttcaaggc ctcgagcacc gcattgccgg tgaggagcga gatctcgcgc tcgctcgagt
      601 cgccgccgag catgaccgcc acgcggccga agtccttcgg gttgctgacg cggcgcacgc
      661 gcgcgggttg gtagctgaga cgcatggcct acggcactcc cacgatgcgc acctcggggt
      721 gcagcgacac gccgtgcacg cgctcgacgg tcgagcgcac atgctcgatg aggcgctcga
      781 ggtcggcggc gcgcgccgtg ccgtggttga tgatgaaatt cgcatgcttc tgcgacaccg
      841 acgcgtcgcc catgcgaaag cccttcaagc ccgcggcctc gatgaggcgc gcggcgtgat
      901 cccccggcgg attggtgaac accgagccac agctccactc gccgatcggc tgcgactcct
      961 tgcgccgcgc cagcagcgag cgcacctcgt cggcgtgcgc gccgggacga tgctcgaagc
     1021 cgagcgtggc gctgatgaac cactcctcgg gcgcgggcgc gtccaccgtg cggtagccca
     1081 cctggtactc gctcgcgtcc cgcatgcgct ccttgccgtg gcggtcgatg gtctcgaggc
     1141 tcagcacgtg ctgccaggtc tcaccgccga aagccccggc gttcatggcg agcgccccgc
     1201 cgagcgtccc ggggatgcct gcgaagaact ccgcgggacc caggccccac ttgatgcact
     1261 ggcgcgcgag gcgcgcacag gcgacgccgg ctccggcgcg cacttgggtc tcgctcaagc
     1321 gctcgagcga atcgagcgtg ccgtgcgtgc tgatgacgac gcccggcagt ccgccgtcgc
     1381 gcaccaggag gttgctgccg agcccgatcc agcagatcgg aacgccttcc gggagtccgc
     1441 gcaggaacgc ggcgagttcc gtccggtcgc gcgggttgaa gaacacctcg gcggggccgc
     1501 ccacgtgcca ggacgtgtgg cggctcaagg gctcgccgcg ccgcacgcgg gtgctgaagc
     1561 cggcggccac cgcgacggtc acgacgcgcc tcccggcggc agcagcgccg cgagcgccgc
     1621 gggcaggcgc tgcgccatgg cg
//
LOCUS       DATLKL010000680         1576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_211143, whole genome shotgun sequence.
ACCESSION   DATLKL010000680 DATLKL010000000
VERSION     DATLKL010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211143"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VM713_09210"
     CDS             <1..550
                     /locus_tag="VM713_09210"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HVH84480.1"
                     /translation="GAEVVLVSGPVSLPTPPGVARIEVESAADMLAAVLRELPGTHVF
                     ISTAAVADYRPACAARQKIKKTSDTLEVSMERTPDVLATVAALAKRPLVVGFAAETES
                     VEQNARAKLMKKNLDMIAANEVGDDKGFDSEENQLIVLARGARYELGRAGKLTLARGL
                     IALIARELEARADRTQRGTALG"
     gene            558..1028
                     /gene="dut"
                     /locus_tag="VM713_09215"
     CDS             558..1028
                     /gene="dut"
                     /locus_tag="VM713_09215"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HVH84481.1"
                     /translation="MSRAARRALKVRILDTRIGGEFPLPAYATEGSAGLDLRACTAAP
                     LTLDPGAAELIPTGLAIHLGDPSLAAVIVPRSGLGHRHGIVLGNLVGLIDSDYQGQLM
                     VSCWNRGSQPYTVTPGERIAQLVVVPVVQVDLEIVPEFETSARGAGGFGHSGRG"
     gene            complement(1070..>1576)
                     /locus_tag="VM713_09220"
     CDS             complement(1070..>1576)
                     /locus_tag="VM713_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84482.1"
                     /translation="DQIPPEYAGKDREIMNPQGVPVKHVDGQKSPDQLAAEARARAAI
                     IKQKQHDTFLVTTYTSVKDIEALRDVRLEQLKTQRAAGQQYIETLKARLGALQARAMG
                     YRPYSAKAAARPLPDDLAEDLVRTLSEMTTQTSALAHVTEEETDLRARFQADINRYRE
                     LHTLHSTD"
BASE COUNT          219 a          534 c          563 g          260 t
ORIGIN      
        1 gggcgcggag gtcgtactgg tgagcggccc ggtgagcctg ccgaccccgc cgggagtcgc
       61 gcgcatcgag gtcgagagcg ccgccgacat gctggccgcg gtgctcaggg agctccccgg
      121 aacgcatgtc ttcatctcca ccgccgcggt cgccgactac cgcccggcgt gcgcggcgcg
      181 gcagaagatc aagaagacca gcgacacgct cgaagtctcc atggagcgca cgccggacgt
      241 gctcgccacg gtggcggcgc ttgcgaagcg gccgttggtg gtggggttcg ccgccgagac
      301 cgagtcggtc gagcagaacg cacgcgccaa gctcatgaag aagaatctcg acatgatcgc
      361 cgccaacgag gtcggcgacg acaagggctt cgacagcgag gagaaccagc tcatcgtgct
      421 cgcccgcggc gcgcgctacg agctcggcag ggcgggcaag cttactctgg cgcgcggact
      481 catcgcgctc atcgcgcgcg agctcgaggc gcgcgccgac cgtacccagc gcggcacggc
      541 gctcggctga ggcccgtatg agccgcgctg cacgccgagc gctcaaggtg cgcatactcg
      601 atacgcgcat cggcggcgag tttccgctgc ccgcctatgc gaccgagggc tcggccggac
      661 tcgatctgcg cgcctgcacc gccgcgccgc tcactctcga cccgggagcc gccgagctca
      721 tcccgaccgg gctcgcaatc cacctgggcg atccgtcgct ggcggcggtc atcgtgccgc
      781 gctcgggcct cgggcaccgc cacggcatcg tgctcgggaa tctcgtcgga ctcatcgact
      841 ccgactacca gggtcagctc atggtgtcgt gctggaatcg cggctcgcag ccctacaccg
      901 tgacccccgg cgagcgcatc gcgcagctcg tggtagtgcc ggtggtacag gtggatctcg
      961 agatcgtgcc ggagttcgag acttccgcgc gtggggccgg aggtttcgga cactcgggcc
     1021 gcggctgagc gcggcgcacg gcgctgcgcg ccgcgctcct cgagggagct cagtcggtcg
     1081 agtgcagcgt gtgcagctcg cgatagcggt tgatatcggc ctggaaccgc gcgcgcaggt
     1141 ccgtttcttc ctcggtcacg tgggcgagag cactcgtctg cgtggtcatc tcgctgagcg
     1201 tgcgcaccag gtcttccgcc aggtcatcgg gcagcgggcg cgccgcggct ttggcgctgt
     1261 agggacggta gcccatggcg cgcgcctgca gcgcgccgag gcgcgccttg agggtctcga
     1321 tgtattgctg tcccgcggcg cgttgcgtct tcaactgctc gaggcgcacg tcgcgcaacg
     1381 cctcgatgtc cttcactgag gtataggtgg tgacgagaaa ggtgtcgtgc tgcttctgct
     1441 tgatgatcgc ggcacgcgcg cgcgcctcgg cggcgagctg gtcggggctc ttctgcccgt
     1501 cgacgtgttt caccgggacg ccctgcggat tcatgatctc gcggtccttg ccggcgtact
     1561 cgggtgggat ctggtc
//
LOCUS       DATLKL010000681         2165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_42066, whole genome shotgun sequence.
ACCESSION   DATLKL010000681 DATLKL010000000
VERSION     DATLKL010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42066"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VM713_09225"
     CDS             <1..661
                     /locus_tag="VM713_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVH84483.1"
                     /translation="PDNPTVMIGFNRRFDPSYRALRERLRLGEIGRLELAVITSRDPA
                     PPPAGYIQSSGGLFRDMTIHDFDTARYLAGDIARVHAFGANLVDAEIGRLGDIDTCAI
                     SLRARSGALIQINNSRRCVYGYDQRIEAFGAGGMLQAGNRHETSVAAWNAERTAGRDV
                     VLHSFIERYAQAYEAEMDAFVRALAEGAPMSPDFTDGVEALRLAVAAGESLRTGRVVD
                     V"
     gene            complement(772..1680)
                     /locus_tag="VM713_09230"
     CDS             complement(772..1680)
                     /locus_tag="VM713_09230"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HVH84484.1"
                     /translation="MSPQALEVAVASEVVFITCAVTGSGDSVRKSPHVPVTPAQIAAA
                     ALEAHAAGAAIVHIHVRDPDSGAPSRATALYREVVDRIRSESAEVILNLTGGMGGDLV
                     LGSDEAPLHFGKGTDFVGPRERIAHIVELRPEIASLDSGSLNFDEALYGTTPRYLRDI
                     AAEYRARGVRPEIEVFELGHIELARQLISEGLIAQPALFQLCLGVRYGAPATAQAMLA
                     MRDALPHGVRWSAFGAGRMQMPMVAQAVILGGNVRVGLEDNLYLGKGVLATNAQLVER
                     AVGIVELLGARVLSSAETRARLKLRA"
     gene            1744..>2165
                     /locus_tag="VM713_09235"
     CDS             1744..>2165
                     /locus_tag="VM713_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH84485.1"
                     /translation="MTNKAGWGMSALPRIVRGEGSYLYDAAGRRYLDGSGGPAAFSIG
                     HGNREVNAAISAQLAQVACGYRYLFTSEPLEELTGLLLTLSGPAFAHIVYSGSGSEAV
                     ESALKVALQYWDARGSSAKRHFIARERSYHGNTLGALSV"
BASE COUNT          343 a          766 c          733 g          323 t
ORIGIN      
        1 gcccgacaac cccaccgtga tgatcggctt caaccgccgc ttcgacccct cctaccgggc
       61 gctgcgcgag cggctcaggc tcggtgagat cggccggctc gagctcgccg tcatcaccag
      121 ccgggatccg gcgccgccgc ccgcgggtta catccagtca tccggcggcc tgttccgcga
      181 catgaccatc cacgacttcg acacggcgcg ttacctcgcc ggcgacatcg ccagggtgca
      241 tgccttcggt gcaaacctcg tcgatgcgga gatcggccgg ctcggcgaca tcgacacctg
      301 cgccatctcg ctgcgtgcca ggtcgggcgc gctcatccag atcaacaaca gccgccgctg
      361 cgtatacggc tacgatcagc gcatcgaggc cttcggtgcg ggcggcatgc tgcaggcagg
      421 caatcgccac gagacctccg tcgccgcctg gaacgccgag cgcaccgcag ggcgcgacgt
      481 cgtgctgcat tccttcatcg aacgctacgc gcaggcctac gaggccgaga tggatgcgtt
      541 cgtgagggcg cttgccgagg gcgcacccat gagcccggac ttcaccgacg gagtcgaagc
      601 gctgcggctc gccgtggcgg ccggggagag cctgcgcacc gggcgtgtgg tcgacgtata
      661 gtttgaagct cgcatcggac gtcctgtcac gatgctcgcg gcggcgcgcc aaaagcgcgg
      721 tttcggcgcg ccgctcgccg cctgcggcgg cgggcacatc cctgtgcctg gctatgcacg
      781 tagcttgagg cgggcgcgcg tctcggcgga cgagagtacc cgcgcgccga ggagctctac
      841 gatcccgacg gcccgctcga ccagctgggc gttggtcgcg agcacgccct tgccgaggta
      901 gagattgtcc tcgaggccga cccgcacgtt accgcccagg atcaccgctt gggcgaccat
      961 cggcatctgc atcctacccg ccccgaaggc cgaccagcgc accccgtgcg gcagagcatc
     1021 gcgcatcgcc agcatggcct gcgcggtcgc aggcgcgccg tagcggacgc cgaggcacag
     1081 ctggaacagc gccggctgtg cgatgagacc ctcgctgatc aactgccggg cgagctcgat
     1141 gtgcccgagc tcgaagacct cgatctccgg gcgcacgccc cgggcgcggt actcggccgc
     1201 gatgtcgcgc aggtagcgcg gcgtcgtgcc gtacaaggcc tcatcgaaat tcagcgagcc
     1261 gctgtcgagg ctggcgatct cgggccgcag ctcgacgatg tgcgcgatac gctcgcgcgg
     1321 gccgacgaaa tccgtgccct tgccgaagtg cagcggtgct tcgtcggatc ccagcaccag
     1381 gtccccgccc atgcctccgg tcagattgag gatgacctcg gcgctctcgc tgcggatgcg
     1441 gtcgacgacc tcacgataaa gggcggtcgc ccggctcggc gcgccggagt ccggatcacg
     1501 cacgtggatg tgcacgatgg ccgcgccggc ggcgtgcgcc tcgagcgccg ccgccgcgat
     1561 ctgcgcgggc gtcaccggta cgtgcgggct cttcctgacg gaatcgcccg agccggtaac
     1621 cgcgcaggtg atgaaaacca cctcgctcgc caccgccacc tccagcgcct gcggactcaa
     1681 gcagcatagc gccaaagccc tcacaatagg gcccgtcgcg agagagttcg aagcgagagg
     1741 atcatgacca acaaggccgg gtggggcatg agcgcgctgc cgcgcatcgt gcgcggggag
     1801 ggcagctacc tctacgatgc cgcgggccgc cgctacctcg atggctcggg gggaccggcc
     1861 gcattcagca tcggccacgg caaccgcgag gtgaacgccg cgatcagcgc acagctcgcg
     1921 caggtggcct gtggttaccg ctatctcttt accagcgagc cgctcgagga gttgacgggc
     1981 ctgctgctca cgctctcagg ccctgcgttc gcgcacatcg tgtactcggg cagcggctcg
     2041 gaggcggtgg agtcggcgct gaaggtggcg ctgcaatact gggacgcccg gggatcttcc
     2101 gccaagaggc acttcatcgc gcgcgagcgc tcctaccacg gcaatacgct cggggcactg
     2161 tcggt
//
LOCUS       DATLKL010000682         1798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_199808, whole genome shotgun sequence.
ACCESSION   DATLKL010000682 DATLKL010000000
VERSION     DATLKL010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199808"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..>1798)
                     /locus_tag="VM713_09240"
     CDS             complement(25..>1798)
                     /locus_tag="VM713_09240"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HVH84486.1"
                     /translation="ESMRSPLKAWVRALERTAAIGRDPTLTLPVVIGRLADRLGDSPA
                     LLAPESRLSYRALAEGANRFARWGLAQRLARGDVICLLTKNCPEYLAIWLGLSRIGVT
                     VALINTNLTGELLRHSIDIAHPRHVIVGASLGSVVVGARARLDAGVVCWSYGDGSLGL
                     PRLDLEVEGLPGTPLGAAECPLPTLAERALYIYTSGTTGLPKAANVSHLRLMQWSHWF
                     AGLADIRESDRLFNCLPMYHSVGGVVATGAALVAGGAVVVRERFSVSSFWRDVIAERC
                     TLFQYIGELCRYLLNAPSETREATHELRLACGNGLRGEVWEPFQRRFRIPHILEYYAS
                     TEGNFSLYNCEGRVGAIGRIPPFLAHRIPVAIVKHDLDTGAPARDVQGRCIPCASGEA
                     GEAIGQILDTDGGTRFEGYADSEASGKKVLRNVFAGGDTWYRTGDLMRRDEQGFFYFV
                     DRVGDTFRWKGENVSTTEVAAAISGCQGVAEVAVYGVAVPKADGRAGMAALVVGADFD
                     LAELRRTVATVLPAYARPVFVRVAAALEITGTFKLRTQELARQGYDPGQVSDALYLDD
                     AARGEYVRLDATLYRRIVTGAQRV"
BASE COUNT          307 a          619 c          602 g          270 t
ORIGIN      
        1 cgggcaacac ggcttaggac ttgttcacac tcgctgcgcc cccgtgacga tccgccggta
       61 gagggtcgcg tcgagccgca cgtactcccc ccgcgcggca tcatccaggt agagcgcgtc
      121 gctcacctga cccggatcgt agccttgtcg ggcgagctcc tgggtgcgga gcttgaaagt
      181 gccggtgatc tcgagcgccg ccgcgacgcg cacgaacacc ggccgcgcgt acgccggcag
      241 cacggtcgcc accgtgcgcc gcagctcggc gaggtcgaaa tctgcgccga cgaccagtgc
      301 cgccatgccg gcgcgcccgt cggccttcgg caccgcgacg ccgtacactg cgacctcggc
      361 gacgccctgg cacccagaga tggcggccgc gacctcggtg gtcgacacgt tctcgccctt
      421 ccagcggaac gtatcgccca cccggtcgac gaaatagaag aatccctgct cgtcccgccg
      481 catgagatct ccggtgcgat accaggtgtc gcccccggca aagacattgc gcaagacctt
      541 tttgcccgag gcctctgagt ctgcgtaacc ctcgaagcgc gtcccgccgt cggtgtcgag
      601 gatttggccg atcgcctcac cggcttcgcc gctcgcgcac gggatgcagc gcccctggac
      661 gtcgcgcgcc ggcgcgcccg tatcgaggtc gtgcttcacg atcgcgaccg gtatgcgatg
      721 tgccaggaag gggggaatgc gacctatcgc gccaacgcgg ccttcacagt tgtagagcga
      781 gaagttgcct tccgtcgagg cgtagtactc taagatatgc ggaatgcgga aacggcgctg
      841 gaagggctcc cagacctctc cacgcaggcc gttgccgcag gcgaggcgca gttcgtgggt
      901 ggcttcgcgc gtctcggacg gcgcgttgag gagatagcgg caaagctcgc cgatgtactg
      961 gaacagcgtg cagcgctcgg caatcacgtc cctccagaaa ctactcaccg agaaacgctc
     1021 gcgcacgact acggcgccgc ccgccaccag tgccgcgccc gtcgctacca cgccgccgac
     1081 gctgtggtac atgggcaggc agttgaagag ccggtcgctc tcgcggatgt cggcgaggcc
     1141 cgcgaaccag tgactccact gcatgaggcg caggtgactc acgttggcgg ccttcggcag
     1201 cccggtcgtc cccgaggtgt agatatagag agcgcgctcg gccagggtcg gcagtggaca
     1261 ttccgccgcg ccgagcggcg tgccaggcag accctcaact tcgaggtcga ggcgcgggag
     1321 gccgagactc ccgtcgccgt agctccaaca gaccacaccc gcgtcgagcc tggcacgcgc
     1381 gccaacgacc acactcccaa gcgaggcgcc cacgatcacg tggcgcgggt gcgcgatgtc
     1441 gatagagtgc cgaagcagct ctccagtgag gttggtgtta atgagagcca cggtgacgcc
     1501 gatgcggctc aacccgagcc agatcgcgag gtactccggg cagttcttcg tcagcagaca
     1561 tatcacgtcg ccgcgcgcga gcctttgagc caggccccag cgggcgaagc gattggcgcc
     1621 ttccgcgaga gcgcgatagg agagccgact ctcgggcgcg agcagcgccg ggctgtctcc
     1681 gaggcgatcg gcgagccgtc cgatcaccac cggcagggtg agcgtgggat cgcggccgat
     1741 cgcggccgtg cgctcgaggg cgcgcaccca ggccttgagg ggtgagcgca tgctttca
//
LOCUS       DATLKL010000683         2543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_155134, whole genome shotgun sequence.
ACCESSION   DATLKL010000683 DATLKL010000000
VERSION     DATLKL010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155134"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1002)
                     /locus_tag="VM713_09245"
     CDS             complement(70..1002)
                     /locus_tag="VM713_09245"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HVH84487.1"
                     /translation="MELPMAVFAAATARLASVSTLRQVAFCGAVWLIYGKLIRFMKSA
                     SSTQVLAAARQWLRPLVHVLIRSGVTWKDFAELARTSYVDVATQQFGKRGRPTNVSRT
                     AVLTGLTRREVRKQRARLAARPASWAGHVTKGSLVLSAWHQDPQFLDARGQPAPLPLE
                     GERVSFATLLRHCGAGDVRPSTLLKELLASHAVRQLPDGRLEPLKRSYIPQSMDEQLV
                     LLWGKRMTDLARTYAHNLTGSPKAAARFERAAVNDRIQASALPEFREFLEREGQAFLE
                     RVDAWLTEHQAGVERADTGRAAIRLGAGLYHIGD"
     gene            1126..2394
                     /locus_tag="VM713_09250"
     CDS             1126..2394
                     /locus_tag="VM713_09250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH84488.1"
                     /translation="MSPFLATWPLLLGMGILMLGAGLQSTLLGVRATIEAFPTIVTGI
                     IMSCYYVGYLAGTRLAPQLLQRVGPVRVFATLAALASVAVLVHGAWVHPAPWALMRLL
                     SGICFAGIYVVAESWLNHRASSSNRGQLLAVYMLVLYVGLGGAQFLLVLSSPQSAEPF
                     MLVSALISAAMVPIVASAQEVRGPDAPQRVRFRDLYRNSPVGVVAVAVSGMISSIIFS
                     MGPVYARLSGFGTRGVAEFMAVSILAAVLTQYPVGRLSDRLDRRTVIASMCTLATFVA
                     AAIEVFGPLPRVAFLAMAASFSGAALTLYSLSVSHVNDKLDSSQIVAASSALLLINGT
                     AAAFGPVLTGGLMAMFGPRAYFGTLGALTGVLALFDLWRKLRREPVPQDQKGRFLNTR
                     ELITSVGLDPAPMAGAEPLELPRARGVQAR"
BASE COUNT          374 a          856 c          846 g          467 t
ORIGIN      
        1 aggaggccag cagcagggcg gctgcggcga agaggatctg ggtcgaacgg tgcatcgctg
       61 atcccccgct caatccccga tgtgataaag cccggcgccg agtctgatcg ccgcgcgccc
      121 cgtgtcggcc cgctcgacgc ccgcctggtg ctcggtgagc cacgcatcga cccgttcgag
      181 gaaggcctgg ccttcccgtt cgagaaactc ccgaaactcg gggagcgcac tcgcctggat
      241 gcggtcattg accgcggcgc gctcgaacct cgccgcagct ttcggggagc ccgtcaggtt
      301 gtgggcatag gtcctggcga ggtctgtcat gcgcttgccc cacaggagca cgagctgctc
      361 gtccatgctc tgcggaatgt aactgcgctt gagcggctcg agtcgcccgt cgggcagctg
      421 gcggaccgcg tgtgaagcca ggagttcctt cagcagagtc gagggccgga cgtcaccggc
      481 gccacagtgg cgcagcagtg tcgcgaagct cacccgttca ccctccagcg gcagcggtgc
      541 gggctggccg cgggcgtcca gaaattgcgg atcctggtgc cacgccgaga gcaccagcga
      601 tcctttggtg acgtgacccg cccagctcgc cggacgtgcg gcgagccgcg cacgctgctt
      661 gcgcacctcg cgtcgcgtca gccccgtgag cacggcggta cgcgagacgt tggtcgggcg
      721 tccgcgcttg ccaaactgtt gggtcgccac gtccacgtag ctcgtacgtg ccagctccgc
      781 gaagtccttc caggtgactc cactgcggat caggacatga accaagggtc gcagccattg
      841 acgggctgcg gctagaacct gagtgctgga cgctgacttc atgaagcgta ttagcttgcc
      901 atatatgagc catactgccc cacagaaggc aacctgtcgc aaggttgaga cgcttgcgag
      961 tctcgcagtg gccgccgcaa acactgccat cggcaactcc atgcaggatc caacctcggg
     1021 ccacgaaccg aagcgcgggt cgctgcgcca cgggtgttga gtacgatcga gcgggtcgcg
     1081 atgcggcaag gggcgccggg cggctgcgta taagatgtcc cgcccgtgag ccccttcctc
     1141 gcgacctggc cgctgctcct cggcatgggc atcctcatgc tgggcgcggg gctgcagagc
     1201 acactgctcg gagtgcgcgc gacgatcgag gcgttcccca cgatcgtgac cggcatcatc
     1261 atgtcgtgtt actacgtggg ttatctcgcc ggtacgcgac tcgcgccgca gctgctgcag
     1321 cgcgtgggac cggtgcgcgt attcgccaca ctggcggccc tggcctcggt ggcggtgctg
     1381 gtgcatggcg cctgggtgca tccggctccg tgggcgctca tgcgcctgct ctcgggcatc
     1441 tgcttcgcgg gcatctatgt ggtggcggag agttggctca atcaccgcgc ctccagcagc
     1501 aaccgcgggc agctgctcgc cgtctacatg ctcgttctct acgtagggct tggcggcgcg
     1561 cagttcctgc tggtgctgtc gagtccgcag tctgccgagc ccttcatgct ggtgtccgcg
     1621 ctgatctcgg cggcgatggt gccgatcgtt gcctcggcgc aggaagttcg tgggccggac
     1681 gcgccgcagc gggtgcgctt ccgcgaccta taccgcaact cgcccgtcgg ggtggtagcg
     1741 gtggccgtct ccggcatgat ctcctccatc atcttctcca tgggcccggt ctatgcgcgg
     1801 ctctcgggct ttggcacgcg cggggttgcc gagttcatgg cagtcagcat cctcgccgca
     1861 gtgctcacgc agtacccggt cggacgactc tccgaccgcc tggaccggcg cacggtgatc
     1921 gcgagcatgt gtacgctcgc gaccttcgtg gcggcggcga tcgaggtgtt cggcccgctg
     1981 ccgcgcgtcg cgtttctggc catggcggcg tcgttctcgg gcgctgcgct gacgctctat
     2041 tcgctctccg tctcgcacgt caacgacaag ctggactcat cgcagatagt cgccgcgagc
     2101 agcgcgctgc tgctcatcaa cggcaccgcc gcggccttcg gcccggtgct gaccggcgga
     2161 ctgatggcga tgttcggtcc gcgcgcctac ttcggcaccc ttggagctct tacgggagtg
     2221 ctcgccctgt ttgacctgtg gcgcaagctg cggcgggagc cggtaccgca ggaccaaaag
     2281 ggtcgattcc tcaacacacg cgagttgatc acatccgtgg ggctggatcc ggcgcccatg
     2341 gccggcgccg agccgctcga gcttccgcgc gcgcggggcg tccaggcgcg ctgaagtgcg
     2401 gacaacgaca cgggaacacg catgtgagcg ttcccgtgtc cccggtgcat ctgcattgag
     2461 agtctcgctt cacccgccgc gtacagggtt ctcccactcg ccaaaaactc cttcggatac
     2521 agctttctaa ccgacgcgac cct
//
LOCUS       DATLKL010000684         2370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_19348, whole genome shotgun sequence.
ACCESSION   DATLKL010000684 DATLKL010000000
VERSION     DATLKL010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19348"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VM713_09255"
     CDS             complement(<1..738)
                     /locus_tag="VM713_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVH84489.1"
                     /translation="MSNDSARNWTLERDAEGLAWLTLDKPGTSANVLSGPVLVELDGL
                     LAALEKQPPRALVVLSAKKSGFVAGADIREFTGITDADSGYVLIRRGQQVLNRLAALP
                     CPSVAAIHGFALGGGLELALACRYRVAVGDERLALGLPEVQLGIHPGFGGTVRSVQVA
                     GVRAAMELMLTGKPVRADKALRLGLVDALTSEAELRSAAATLVLKSPPPRSAPLLEQL
                     LSSAAPRPLVRRSLIAQVARRAPPGHYP"
     gene            complement(728..1528)
                     /locus_tag="VM713_09260"
     CDS             complement(728..1528)
                     /locus_tag="VM713_09260"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HVH84490.1"
                     /translation="MRLLVYNIRYATGTGPAFHLPLPGAGYLRSNRRVLNGITEFIRD
                     EQPDVVGLLEVDTGSIRTGMVNQAEHIARELGHYSTFQCKYGSGSINTFMPIVRKQAN
                     AFLSGPNVAGERFHYFDTGIKRLIIELELTDVCVFLVHLSVKYRHRQYQLRSLHDLVV
                     QSAKPVIVAGDFNTFWGTHELFLFMRAAGLRTANTTGLPSFPARVPRIELDFILVSEG
                     IEVTGFRVPDVRFSDHRPLVCDFEVLARAGAAMEKVAAASAQSITHEQ"
     gene            1648..2100
                     /locus_tag="VM713_09265"
     CDS             1648..2100
                     /locus_tag="VM713_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84491.1"
                     /translation="MSQVNPVRLFVTHAWANSDDYLRVFEYLESARNFFYKNYSTPDK
                     RPSGDKEALREDLRHQIAPVEAVIALSSLYAAQQDLLTFQLLYAQANHKPVVLLKPFG
                     GSGEAPKAVLDLVDEVVAWDERALVDAIRRQARHEETTRWDTIEFKLD"
     gene            complement(2105..2251)
                     /locus_tag="VM713_09270"
     CDS             complement(2105..2251)
                     /locus_tag="VM713_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84492.1"
                     /translation="MREYLTDEAHALAATEGGVALNGAARGTFNAHAVAGRARNGTSQ
                     LIAL"
BASE COUNT          407 a          777 c          828 g          358 t
ORIGIN      
        1 cgggtagtga ccgggcggcg cgcggcgggc cacctgcgcg atcagcgagc ggcgcaccag
       61 cggtcggggc gccgccgagg agagcagttg ctcgaggagc ggcgcactgc gcggcggcgg
      121 gctcttcagc accagcgtgg ccgcggcgct gcgcagctcg gcctcggacg tcagcgcatc
      181 caccaggccc aagcgcagcg ccttgtcggc gcgtaccggc ttcccggtca gcatcagctc
      241 catcgcggcg cgcacgcctg ccacctggac gctgcgcacc gtgccaccga atcccgggtg
      301 aattccgagc tgcacctccg gaagcccaag cgcgagccgc tcatcgccca ccgcgacccg
      361 gtagcggcag gcgagggcga gctcgagtcc cccgccgaga gcaaagccgt ggatcgcggc
      421 cacgctcggg cagggaagtg cggcgagacg gttgagcacc tgctggccgc ggcggatcag
      481 cacataaccg ctgtcagcat cggtgatgcc ggtgaattcg cggatgtcgg cgccggcgac
      541 gaagccgctc ttcttggccg agagcaccac cagcgcccgc ggcggctgct tctcgagagc
      601 ggccagcagc ccgtcgagct cgacgagcac ggggccggag agcacgttag ccgaggtgcc
      661 gggcttgtcg agcgtcagcc aggcgaggcc ctcggcgtcc cgctcgagag tccagttgcg
      721 cgcgctgtca ttgctcatgg gtgatgctct gggcggacgc ggccgccacc ttttccatag
      781 cggcgcccgc gcgggcgaga acctcgaagt cgcacaccag cgggcgatgg tccgagaagc
      841 gcacgtcggg cacgcggaag cccgtgacct cgatcccctc gctcaccagg atgaaatcga
      901 gttcgatccg cggcacgcgc gccgggaagg agggcaggcc cgtggtgttg gcggtgcgca
      961 ggcccgcggc gcgcatgaac aggaacagct cgtgcgtgcc ccagaaggtg ttgaagtcgc
     1021 cggcgacgat caccggcttg gccgactgca cgaccaggtc gtggagcgag cgcagctggt
     1081 actggcggtg gcggtatttc accgacaggt gcaccaggaa cacgcacacg tcggtgagct
     1141 cgagctcgat gatgaggcgc ttgatgccgg tatcgaaata atggaagcgc tcgcccgcca
     1201 cgttcgggcc cgacagaaac gcattggcct gcttgcgcac gatcggcatg aaggtattga
     1261 tcgatccgct gccgtacttg cactggaagg tcgagtagtg gccgagctcg cgggcgatgt
     1321 gctcggcctg gttcaccatc ccggtgcgga tcgagccggt gtcgacctcg agcagcccga
     1381 cgacgtcggg ctgctcgtcg cggatgaact cggtgatacc gttgagcacc cggcggttgc
     1441 tgcgcaggta gccggccccc ggcagcggca ggtggaaggc tgggcccgtg ccggtggcat
     1501 aacgaatgtt gtagacgagc aggcgcacga gtcgggcatt ctatcccgca ggcagccgtc
     1561 gtgcggcggc gacgagtggc tgcgcacttg cgctccgcgg cccgtgtgcg agacttagct
     1621 cccggtacca cccactaggg ggttccgatg tcgcaagtca accccgtgcg attgttcgtc
     1681 acgcatgcct gggcgaacag cgacgactat ctgcgcgtgt tcgagtacct cgagagcgcg
     1741 cgtaatttct tctataagaa ctacagcacg ccggacaagc gcccgagcgg ggacaaggaa
     1801 gcgctgcgtg aggacctgcg gcaccaaatc gccccggtgg aggcggtgat tgcgctgtcg
     1861 agcctgtacg ccgcgcagca ggatctcctc accttccagc tgctgtacgc gcaggcgaac
     1921 cacaagcccg tggtgctgct caagcccttc ggcgggagcg gcgaggcacc caaggcggtc
     1981 ctcgatctcg tcgacgaggt ggtggcctgg gacgagcgcg cgctggtcga tgcgatccgc
     2041 cgccaggcgc gtcacgaaga gacgacgcgc tgggacacca tcgaattcaa gctcgactag
     2101 cgagctacag cgcgatcagc tgcgatgtgc cgttcctggc acgcccggcc acggcatggg
     2161 cgttgaaggt gccgcgcgcg gcaccgttca gcgcgacgcc gccctcggtt gcggcgagcg
     2221 cgtgcgcctc gtcggtgagg tactcgcgca cgatccgatc tcactcatgc ggatttgatc
     2281 gacctcacaa gcaactactg aaccgtgggc tgcgggagaa ggcgcagcta tcctcttcgc
     2341 acagccgtag ttcatgaaca tcaccaaaaa
//
LOCUS       DATLKL010000685         2658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_121083, whole genome shotgun sequence.
ACCESSION   DATLKL010000685 DATLKL010000000
VERSION     DATLKL010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121083"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..631
                     /locus_tag="VM713_09275"
     CDS             149..631
                     /locus_tag="VM713_09275"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HVH84493.1"
                     /translation="MTNLQEVLMYSSAVMAFLHHLAAFTTVAALAVEVAMFKPPLTLV
                     QARRLQRTDLIFGVSATAVLIIGSLRVAYFEKPPVYYLHDLYFIAKLAAFLIAALISI
                     YPTIVFLSWRTALRAGTAPEVSAERTRRVRLCLMLELTLIVVILACAALMARGFGYMR
                     "
     gene            complement(628..>2658)
                     /locus_tag="VM713_09280"
     CDS             complement(628..>2658)
                     /locus_tag="VM713_09280"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVH84494.1"
                     /translation="DAVIVMDGIDRTQVQSIFTPNVRYLEVVEDGIEAGDVTPADFTP
                     TPENFARVRDNIRRAGIIGRLVANDFSGALVSAIVLDRDAQGRRVDPIAVAHALEREV
                     RAPIEAHGIDVHMIGFAMVVGDIADGAAAVVMFAGITLLLTLLAVRVYIQSWRIAFVP
                     VLCSVVAVIWQLGSLVVLRYGIDPIGLLVPFLIFAIGVSHGVQKISAAADAALAGLDS
                     EAAARRVFRQLLMPAVIALLADLVGFVTILMIPVQVIREMAITASIGVAIVILTDLVL
                     LPVLVSYVHFDPGYRGRVERRQRRLAVLWHRMSALTERGPALVIIVIAALLGAAGWWK
                     GRETPIGDTQAGVPELRASSRYNRDNDVITRKFSIGVDVLTAIVESEQPLCLSHDQMT
                     AVDRFGWRMRNVAGVQEVITLPFIAKVASAGWNEGSLKWRSIPRERTQLNQSTRYIET
                     STGLLDENCDVVPVLNFLTDHRAATIERAVAAVKEWRAQNPLPAAQVRLATGNVGVMA
                     ATNETVRAKELLILGGVFGAVIVMCQLTFRSVTGTVVVVVPLALVSVLVYAVMALVGI
                     GLKLSTLPMVALGAGIGVDYGIYLFSRMQEFLHLGLSVRESYERTLRVTGASIIFTGI
                     TLAIGVATWVFSPLKFQADIGIMLTFMFLVNMLAAIILLPALAAWLIRPRPL"
BASE COUNT          451 a          930 c          884 g          393 t
ORIGIN      
        1 gcggagctca tccgccgctc gctcgccgca ccgaaggcac gtcgcgccac cgcgccgacc
       61 cgcgccgcgc gcgagcgccg cctcgaacgc aaggcccgcg tgcagcgtgc caagcggctg
      121 cggaccaggc cgcgctggga cgaatgagat gaccaatctt caggaggtac tcatgtattc
      181 gtccgccgtg atggcgttcc tccatcacct ggccgccttc accacggtcg cggcgctcgc
      241 ggtggaggtc gcgatgttca agccgccgct gacgctcgta caggcgcggc ggctgcagcg
      301 caccgatctc atcttcgggg tgtcggccac cgccgtgctc atcattggaa gcctgcgggt
      361 cgcctacttc gagaaacccc cggtctacta cctgcacgac ctctacttca tcgccaagct
      421 ggcggccttc ctcatcgccg cgttgatctc gatctatcct acgatcgtct tcctgtcatg
      481 gcggacggcg ctcagggcgg gtacggcccc cgaagtgagc gccgagcgca cccgccgcgt
      541 gcggctgtgc ctcatgctcg agctcaccct gatcgtggtg atcctcgcgt gtgcagcgct
      601 catggcgcgc ggcttcggct acatgcgcta gagcggccgc ggacgaatca gccaggcggc
      661 gagcgccggc aggaggatga tcgccgcgag catgttcacc aggaacatga aggtcagcat
      721 gatgccgatg tcagcctgga acttgagcgg cgaaaacacc caggtggcga ccccaatggc
      781 gagggtgata ccggtgaaga tgatgctggc gccggtgacg cgcagcgtgc gctcgtagga
      841 ctcgcgcacc gacagtccca ggtgcaggaa ctcctgcatg cggctgaaga gatagatgcc
      901 gtaatcgacg ccgatgccgg cgccgagcgc caccatcggc agcgtcgaga gcttcaagcc
      961 gatgccgacc agcgccatca ccgcgtacac gagcaccgac accagcgcca gcggcacgac
     1021 caccaccacg gtgccggtga ccgagcggaa ggtcagctgg cacatgacga tcaccgcgcc
     1081 gaacacgccg ccgaggatca gcagctcctt cgccctgacc gtctcgttgg tcgccgccat
     1141 gaccccgacg ttgccggttg ccagccgcac ctgggctgcg ggcagcggat tctgcgcgcg
     1201 ccattccttg accgcggcga cggcgcgctc gatggtcgcg gcgcgatgat cggtgaggaa
     1261 attgagcacc ggaacgacgt cgcagttctc atcgaggagc ccggtgctgg tctcgatgta
     1321 gcgggtcgac tggttgagct gggtccgctc gcgcgggatg ctgcgccact tcaggctccc
     1381 ctcgttccag ccggcgctcg ccaccttggc gatgaagggc agcgtgatga cctcctgcac
     1441 cccggcgacg ttgcgcatgc gccagccgaa gcgatccacc gccgtcatct ggtcgtggct
     1501 gaggcacagc ggctgctccg actcgacaat cgcggtcagc acgtcgacgc cgatcgagaa
     1561 cttccgggtg atcacgtcgt tgtcgcggtt gtagcgcgag ctggcgcgca gctccggcac
     1621 gccggcctgc gtgtcgccga tcggggtctc gcgccccttc caccagccgg ccgccccgag
     1681 gagcgccgcg atcacgatga tgacgagcgc cgggccgcgc tcggtgagcg ccgacatgcg
     1741 gtgccagagc acggccagcc gccgctggcg ccgctcgacg cggccgcggt aaccgggatc
     1801 gaaatgcacg taggacacca gcaccggcag cagcaccagg tcggtgagaa tcacgatcgc
     1861 cacgccgatg ctggcagtga tcgccatctc gcggatcacc tgcacgggga tcatgagaat
     1921 cgtcacgaag ccgaccagat cggcgagcag cgcgatgacc gccggcatca gcagctggcg
     1981 gaacacccgg cgcgcggccg cctcgctgtc gagcccggcg agcgccgcat ccgccgcagc
     2041 gctgatcttc tgcacgccgt ggctcacgcc gatcgcgaag atcaggaacg gcaccaggag
     2101 cccgatcggg tcgatcccgt agcgcagcac cacgagtgag ccgagctgcc agatcacggc
     2161 cacgaccgag cacagcaccg gcacgaaggc gatgcgccac gactggatgt agacccgcac
     2221 cgcgagcagg gtcaggagca gggtaatgcc cgcgaacatc accaccgcgg cggcgccgtc
     2281 ggcgatgtcg cccaccacca tggcgaagcc gatcatgtgg acgtcgatgc cgtgtgcctc
     2341 gatgggtgcg cgcacctcgc gctcgagcgc atgcgcgact gcgatcggat cgacgcgccg
     2401 tccctgcgcg tcccggtcga gcacgatggc gctcaccagc gcgcccgaga aatcgttggc
     2461 gaccaggcgc ccgatgatcc cggcgcggcg gatgttgtcg cgcacccgcg cgaagttctc
     2521 cggcgtgggc gtgaagtctg ccggcgtgac gtcgcccgcc tcgatgccgt cctcgaccac
     2581 ctcgaggtag cggacgttgg gagtgaagat cgactgcacc tgggtgcggt cgatgccgtc
     2641 catgacgatg acggcgtc
//
LOCUS       DATLKL010000686         1616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_121085, whole genome shotgun sequence.
ACCESSION   DATLKL010000686 DATLKL010000000
VERSION     DATLKL010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121085"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..739)
                     /gene="rpoE"
                     /locus_tag="VM713_09285"
     CDS             complement(134..739)
                     /gene="rpoE"
                     /locus_tag="VM713_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HVH84495.1"
                     /translation="MGADASDLSLVRRVQRGDKGAFDALVLKYQHKLVKLVMRYVRNP
                     AEAEDIAQEAFIKAYRALPQFRGDSAFYTWLYRIAINTAKNAVVSRDRSPIEFSNDRN
                     SEESYEMQGRMKDSETPEALVLTDEIRSIVNAAIDALPEDLRTAIVLRELEGLSYEEI
                     AAAMDCPVGTVRSRIFRAREAIDRRLREVFEGGLGRAEELR"
     gene            complement(809..1108)
                     /locus_tag="VM713_09290"
     CDS             complement(809..1108)
                     /locus_tag="VM713_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HVH84496.1"
                     /translation="MAREPPESRAPGAAPAATPGRLLWRCRRGMKELDVILERFAREG
                     LPGASPAERRAFERLLGLPDPLLAGYFLGAEHPADPELRALAARIRDLCHAAPVC"
     gene            complement(1113..>1616)
                     /locus_tag="VM713_09295"
     CDS             complement(1113..>1616)
                     /locus_tag="VM713_09295"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HVH84497.1"
                     /translation="GINRLACTTSMQDLGAVIRIYPLPHMPVIKDLVPDLSNFYAQYA
                     AVKPWLQARTPPPPDHERLQSKEEQEQIDRPAACILCACCSTACPSYWWNSERFLGPA
                     ALLAAYRWIIDSRDEATGERLDELEDPFRLYRCHTIMNCTDVCPKDLNPARAIAEIKK
                     MLVERQG"
BASE COUNT          242 a          546 c          559 g          269 t
ORIGIN      
        1 gcgatcaccg cgtagcgctc ccagcgggcc ttgagctcgc cgtcgcgcga gaggcggcgg
       61 gcgagcagct cgcactcggc ggacgccagc tcgtcgtcga acatggcgga caactggctg
      121 tcgagctcct cgttcatctc aactcctcgg cgcggcccag gccgccctcg aacacctccc
      181 gcaggcgtcg gtcgatcgcc tcgcgggcgc ggaatatgcg cgagcgcacc gtccccaccg
      241 gacaatccat ggcggcggcg atctcctcgt acgacagccc ctcgagctcg cgcagcacga
      301 tcgccgtgcg caggtcctcg ggcagcgcat cgatggcggc gttgacgatc gagcggatct
      361 cgtccgtcag caccagcgcc tcgggggtct cggaatcctt catgcggcct tgcatctcgt
      421 acgactcttc gctgttgcgg tcgttgctga attcgatcgg gctgcggtcg cgggacacta
      481 cggcgttctt ggcggtgttg atcgcgatgc ggtacagcca ggtatagaag gcgctgtcgc
      541 cgcggaattg cggcagggcg cggtaggcct tgatgaaggc ctcctgggcg atatcctcgg
      601 cttcggctgg gttgcggacg tagcgcatga ccagcttgac gagtttgtgc tggtacttga
      661 ggaccagcgc atcgaatgcc cccttgtctc cgcgttgcac gcggcgtacc aggctgagat
      721 cactcgcatc ggcgcccatg cgccccctac cccggcagag agcgccgcca cgccgcgcgc
      781 tggatagact gtgacccctt gtaaaagttc agcataccgg agcagcgtga cataagtccc
      841 ggatgcgcgc cgccagcgcg cgcagctcgg ggtcggcggg atgttccgcg ccgaggaaat
      901 agcccgccag caggggatcc ggcagcccga gcaggcgctc gaacgcccgc cgctccgccg
      961 gggaggcccc cgggagcccc tcgcgcgcga agcgctcgag aatcacgtcg agctccttca
     1021 tgccgcgccg gcagcgccac aggagtcgcc ccggtgtcgc ggcgggcgct gcgcccggcg
     1081 cgcgcgactc cggtggctcc cgagccatcg ggtcagccct ggcgctcgac cagcattttc
     1141 ttgatctccg cgatggccct ggcgggattg agatccttcg ggcaaacgtc ggtgcagttc
     1201 atgatggtat ggcaccggta gagccggaag gggtcctcga gctcatcgag ccgctcgccg
     1261 gtcgcctcgt cgcggctgtc gatgatccag cggtacgcgg cgagcagcgc cgcgggaccc
     1321 aggaaccgct cgctgttcca ccagtaactc gggcaggcgg tcgagcagca ggcgcagagg
     1381 atgcaggccg ccggccggtc gatctgctcc tgctcctcct tgctctgcag cctctcgtga
     1441 tcgggcggcg gcggggtgcg cgcctggagc cacggcttca cggcggcgta ctgcgcatag
     1501 aagttgctga ggtccggcac cagatccttg atgaccggca tgtgcggcag cggatagatg
     1561 cggatcacgg cgccgagatc ctgcatcgag gtggtgcagg cgaggcggtt gatgcc
//
LOCUS       DATLKL010000687         3348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_199856, whole genome shotgun sequence.
ACCESSION   DATLKL010000687 DATLKL010000000
VERSION     DATLKL010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199856"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VM713_09300"
     CDS             complement(<1..509)
                     /locus_tag="VM713_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84498.1"
                     /translation="MLSEVAVRAELQPMHARCTDARRSRPGARLACAAWLAAAAGSAH
                     ADDLLGFYLGGTDGKATIDASTGLVSGFGVHSSAYTIIAGWRPRPELSAELQYFDFGH
                     SSGTTDYRNSNVPLTNSASRKGEGAFGILYLPTPIVDFYMKAGLAKLHTTATTIVGTC
                     PKGGVCPPLS"
     gene            complement(602..>3348)
                     /gene="pyc"
                     /locus_tag="VM713_09305"
     CDS             complement(602..>3348)
                     /gene="pyc"
                     /locus_tag="VM713_09305"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HVH84499.1"
                     /translation="MQRRNQKVVERAPAVFLSARGRAELAAAALAIGRAVRYRNAGTV
                     EFLEDADSGRCYFIEVNPRIQVEHTVTEEVNGIDLVKAQIRLAGGARIGTPESGVPAQ
                     EEIRVSGHALQCRITTEDPENNFIPDYGKITAYRSPAGFGIRLDAGTAYTGAIITRSY
                     DSLLVKVTAWAPTPEETIARMHRALWEFRIRGVVTNLRFLDQLITHPRFAPGEYTTRF
                     IDETPELYRWPLKRDRATRLLTGIGATIVNGNAQVRGRPRPAHFALPQPPPGPLPAPA
                     PGTKQKLDELGPERFARWMLAEERVLLTDTAMRDAHQSLLATRLRTADLIAIAPAYAS
                     LTPELFSLECWGGATFDVALRFLHEDPWERLGQLRERVPNILLQMLLRSANAVGYANY
                     PDNVLRFFIARAAARGIDLFRIFDCLNWVENMRVAIDAVLESGRLCEAALCYTGNLSD
                     PREKKYTLDYYLGLARELKAAGTHVLGIKDMGGLIRPRAAYTLVRALKEEIGLPLHFH
                     THDTSGIGAASVLAAIDAGADAVDGAIDALSGLTSQPNLGSIVEALRFGPRDARLDPD
                     HLRAISSYWEQARRLYVAFESDVRAGASEVYVHGMPGGQYTNLREQARALGVSEARWP
                     EVARAYADVNRAFGDIIKVTPTSKAVGDLALVMVTAGLTSEQLLDPGTDVAFPESVVQ
                     FFRGELGQPYGGFPAALQRKVLQGTASLTERPGAGAPAVDLEAERARLAERLPGAISD
                     EDLASYLMYPRVWQDYARERLQHGDMGLLPTPVFFYGMQPGDEISIDLERGKTLIVRY
                     IATSEAHEDGTRTVFFELNGQPRSVRVRDSSRVAQRPPARKAESGNPRHVGAPMPGTV
                     TTVAATVGARVARGDLLLTLEAMKMETAVRAESEGEVAEVLVKPGEPVDLKDLLIVLR
                     "
BASE COUNT          490 a         1140 c         1207 g          511 t
ORIGIN      
        1 gacagcggcg gacacacccc gcccttcggg caggtgccga cgatcgtggt cgcggtcgtg
       61 tggagcttcg cgagcccggc cttcatgtag aaatcgacga tcggtgtcgg cagatagagg
      121 atgccgaagg ctccctcgcc cttgcggctc gcgctgttgg tcagcgggac gttggaattg
      181 cggtaatcgg tcgtgccgct cgagtggccg aagtcgaagt actgaagctc cgccgacagc
      241 tctggtctcg gacgccagcc ggcgatgatc gtgtaggcgg agctgtggac gccgaaaccg
      301 ctcaccagcc cggtcgaggc gtcgatcgtc gccttgccgt cggtgccgcc gagatagaag
      361 ccgagcagat cgtccgcgtg cgcgctgccg gccgcggccg ccagccatgc ggcgcacgcc
      421 aggcgtgcgc cgggtctcga tcgccgcgca tcggtgcacc gcgcgtgcat cggctgcaac
      481 tccgctcgaa ccgcaacttc cgacagcata cagcaaggat gttggtatcg agcgatgacg
      541 ccaggcgccg acaagggcgc aggcgtgagg gcgggcacga cgcggtgggt ccgcgcacgc
      601 gtcagcgcag cacgatgagc agatccttca ggtcgacagg ctctccgggc ttcaccaaca
      661 cctcggccac ctcgccctcg ctctcggcgc gtaccgcggt ctccatcttc atcgcctcga
      721 gggtcagcag caggtcaccg cgcgcaaccc gggctcccac ggtcgcggcg acggtggtca
      781 ccgtgcccgg catcggtgcg cccacgtggc gggggttgcc ggactcggct ttacgggccg
      841 gcgggcgctg cgccacccga ctcgagtcgc gtacccgcac cgagcgcggc tggccgttca
      901 gctcgaagaa caccgtgcgg gtgccgtcct cgtgcgcctc gctggtcgcg atgtagcgca
      961 cgatgagcgt cttgccgcgc tcgaggtcga tgctgatctc gtctccgggc tgcatgccgt
     1021 agaagaacac cggcgtcggc aggaggccca tgtcgccgtg ctgcagccgc tcgcgcgcgt
     1081 agtcctgcca tacccgcggg tacatgagat aagaggcgag atcctcgtcg ctgatggcgc
     1141 cgggcagccg ctccgcgagc cgcgcgcgct ccgcctcgag atccaccgcc ggcgcaccag
     1201 cgcccggccg ctcggtgagc gacgccgtac cctgcagcac cttgcgctgc agcgccgccg
     1261 ggaagcctcc gtacggctgc ccgagctcgc cgcggaagaa ctgcacgacc gactcgggaa
     1321 aggcgacatc ggtgccgggg tcgagcagct gctcgctcgt caagccggcg gtgaccatga
     1381 cgagcgcgag gtcacctact gccttggagg tcggggtcac cttgatgatg tcgccgaacg
     1441 cccggttgac gtctgcgtag gcgcgcgcca cctccggcca gcgtgcctcg ctgacgccga
     1501 gggcccgcgc ctgctcgcgc aggttcgtgt actggccgcc gggcatgccg tgcacgtaga
     1561 cctcggaggc gccggcgcgc acatcgctct cgaaggccac gtacagtcgc cgcgcctgct
     1621 cccagtagga agagatggcg cgcaggtggt cgggatcgag ccgcgcatcg cgcggcccga
     1681 agcgcagcgc ctcgacgatc gagccgagat tcggctgcga ggtgagcccc gacagcgcat
     1741 cgatcgcgcc gtcgaccgca tcggcgcccg catcgatggc ggccagcacg ctcgccgcgc
     1801 cgatgccgct cgtgtcgtga gtgtggaaat gcagcggcag gccgatctcc tccttgagcg
     1861 cccttaccag cgtgtacgcc gcgcgcggcc ggatgaggcc gcccatgtcc ttgatgccga
     1921 gcacgtgggt gccggcggcc ttgagctcgc gcgcgagccc gaggtagtaa tcgagcgtgt
     1981 acttcttttc gcgcggatca ctcaggttcc ccgtgtagca gagcgccgcc tcgcacagcc
     2041 tgcctgactc gagcaccgca tcgatcgcga cccgcatgtt ctcgacccaa ttgaggcagt
     2101 cgaaaatgcg gaacaggtcg atgccgcgcg cggcggcgcg cgcgatgaag aagcgcagca
     2161 cgttgtcggg gtagttggcg tagccgaccg cgttcgccga gcgcagcagc atctgcagca
     2221 ggatgttggg aacgcgctcg cgcagctggc cgagccgctc ccagggatcc tcgtgcagga
     2281 agcgcagcgc cacatcgaag gtcgccccgc cccagcattc gagcgagaag agctcgggcg
     2341 tgaggctcgc ataggcgggc gcaatggcga tgaggtcggc ggtccgcagc cgcgtcgcca
     2401 gcagcgactg gtgcgcatcg cgcatcgccg tgtcggtgag gagcacgcgc tcctccgcga
     2461 gcatccagcg cgcgaagcgc tccggcccca gctcgtcgag cttctgcttc gtgcccggcg
     2521 cgggggcggg cagcggccct ggcggcggct gcggcagggc aaagtgcgcc gggcgcgggc
     2581 ggccccgcac ctgggcgttg ccgttgacga tggtggcgcc gatgccggtg aggagccgcg
     2641 tggcgcgatc gcgcttcaac ggccagcggt agagctccgg cgtctcgtcg atgaagcgcg
     2701 tggtgtactc gccgggcgcg aagcgcgggt gggtgatcag ctggtcgagg aagcgcaggt
     2761 tcgtgaccac gccgcggatg cggaattccc acagcgcgcg gtgcatgcgc gcgatggtct
     2821 cctcgggcgt cggcgcccag gccgtgacct tgacgaggag ggagtcgtag gagcgcgtga
     2881 tgatcgcgcc ggtgtaggcg gtgccggcgt ccaggcggat gccgaagccg gcgggactgc
     2941 ggtaggcggt gatcttgccg tagtcgggga tgaagttgtt ctcggggtcc tcggtggtga
     3001 tgcggcactg gagggcatgc cccgacacac ggatctcctc ctgtgccggt acgccgctct
     3061 ccggggtgcc gatgcgcgca ccgccggcga ggcggatctg cgccttcacc aggtcgatgc
     3121 cgttgacctc ctcggtcacg gtgtgctcga cctggatgcg cggattcacc tcgatgaagt
     3181 agcagcggcc gctgtcggcg tcctcgagga actcgacggt gccggcattg cggtagcgca
     3241 ccgcgcggcc gatggcgagc gccgcggcgg cgagctccgc gcgcccacgc gcgctgagga
     3301 ataccgcagg cgcccgctcc accaccttct ggttgcgccg ttgcatgc
//
LOCUS       DATLKL010000688         2538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_42154, whole genome shotgun sequence.
ACCESSION   DATLKL010000688 DATLKL010000000
VERSION     DATLKL010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42154"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..2366
                     /locus_tag="VM713_09310"
     CDS             156..2366
                     /locus_tag="VM713_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVH84500.1"
                     /translation="MNERGKITAGHLSRQAIVYLRQSSPAQVEHNRESTERQYALASK
                     ARELGWPAERIVVIDEDLGLSGGGTATRSGFARLTAEVALARVGLVLGLEVSRLARNN
                     AEWYRLIDLAGLTDTLIGDGDGLYHPAVFNDRLLLGLKGIMSEAELHILRARLNGGIR
                     NKAARGELRRGLPVGFIWGEEEGEVCFHPDEAVCTAIRQVFAQFAETGSARRVWLWFR
                     SEGLKFPLQMHQGGEIRWVEASYTAIHQVLTNPVYAGAYVYGRTRHEIVLDATGGRKK
                     RVRHLPRAEWQVLIPEHHPGFIDWQTYEANQERIARNTKPGPHQAGGAVREGGALLQG
                     LARCGHCGRRLHTHYRGRNASPGYHCPGKVLVENRGVYCLNIGGIQIDEAVTGSFVAA
                     LEPAGLAATLAAAERLEVEREAALKQWQLGVERANYEAQLAERRYRAVDPDNRLVARG
                     LERDWEERLRALEAAKIELERREKERPRVISQAERDRLLALGPDLAAVWNAPTTTPRD
                     RKELLRTLIEDVTIKVDRDKAATHLTLRWKGGALNEVDLTLPRSRPATVRTDEDTIAL
                     LRRLAAHYPDGVIAGIFNRQGRATAYGHRFTAGRVGNLRRHWGIPCFEQTANAPEGEL
                     MTVKKAAVVLGVAPSTIHRLLNDGIIAGEQPTPGAPWRIRLTKDLKDRFHGDAGEDFL
                     PMREAMRALGVSRQTVLQRVKRGELAAVHVMRGQQKGLRIKVIGRQPDLFHPIS"
BASE COUNT          471 a          843 c          796 g          428 t
ORIGIN      
        1 ccctcccgat ctgccgtcaa gccttacccc gtcgagcccg tcgtcggccg cctgggatca
       61 gctcgacgaa ggcgcccaga tggctgcgct caagctgctg gcacgcctca tcgcgcggat
      121 gatggcagca aacgcgccga taaaggaggc aggtcatgaa tgagcgtggg aagatcacgg
      181 ccggccacct ctcgcgccaa gctatcgtct atttgcgcca atcgagtccg gcgcaagtcg
      241 agcacaatcg cgaatcgact gagcggcaat acgcgctggc cagcaaggcc cgcgagctcg
      301 gctggcccgc cgaacggatc gtcgtcattg atgaagacct gggtctgtct ggcggcggaa
      361 ccgcgacccg ctccggcttt gcccggctca ctgccgaggt ggcgctcgcc cgtgttggcc
      421 tcgtgctcgg tctggaagtc tcacgcttgg cccgcaacaa tgccgaatgg tatcgcctca
      481 tcgatctcgc cggcctcacc gacacgctga tcggcgatgg cgacggcctc tatcacccgg
      541 cggtgttcaa tgacaggttg ttgctggggt tgaagggcat catgagcgaa gcagagttgc
      601 acatactgcg cgcgcgcctc aatggcggca ttcgcaacaa ggcggcgcgc ggcgaactcc
      661 ggcgtggctt gcccgttggc tttatctggg gcgaggagga gggcgaggtt tgttttcacc
      721 ctgatgaagc cgtctgcacc gccatccgac aggtctttgc ccaattcgcc gagaccggct
      781 cggcccggcg cgtgtggctg tggttccgct cggaggggct caaattcccg ctgcagatgc
      841 atcaaggcgg cgagatccga tgggtcgagg cgagctacac cgccatccac caggtgctca
      901 ccaaccccgt ctacgctggc gcttacgtct atggcagaac ccgccacgag atcgtcctcg
      961 atgccaccgg cgggcgcaag aagcgcgtgc gacaccttcc acgcgccgaa tggcaggtgc
     1021 tgatcccgga gcatcatccg ggcttcattg actggcagac ctatgaggca aaccaggaac
     1081 gcatcgcccg caatacaaag cccggaccgc accaggcggg cggcgccgtg agggagggcg
     1141 gcgcacttct gcaagggctg gcccgctgtg gccattgcgg tcgccgtctg cacacccact
     1201 atcgtggtcg caacgcttcg ccaggttacc actgcccggg caaggttctc gtcgagaacc
     1261 gcggcgtcta ttgcctcaat atcggcggca tccagatcga cgaggccgtc actgggagct
     1321 tcgtcgctgc cctggagccg gctgggcttg ccgccaccct tgccgccgct gagcggctgg
     1381 aagtcgaacg cgaagccgct ttgaagcagt ggcagctcgg ggtcgagcga gccaattacg
     1441 aggcgcaact cgccgagcgt cgttatcgcg ctgtcgatcc cgacaatcgg ctggtggcgc
     1501 gcggcctcga acgcgactgg gaggagcgcc tgcgcgcact tgaggcggcc aagatcgagc
     1561 tggaacgccg cgaaaaagaa aggccgcggg tgatctcgca ggccgagcgc gatcgtctcc
     1621 tcgccctcgg acccgatctc gccgccgtct ggaacgcgcc aacgaccacg ccgcgcgaca
     1681 ggaaggagct gctacgcacc ctcatcgaag acgtcacgat caaggtcgat cgcgacaagg
     1741 cggccaccca tctcacgctg cgctggaagg gcggcgcgct caacgaggtc gaccttactc
     1801 tgccgcgctc gcggccggcc accgtccgca ccgacgagga cacgatcgcg ctgctgcgcc
     1861 ggctcgcggc ccattacccc gatggtgtga tcgctggcat cttcaatcgg cagggacggg
     1921 ccacggcata tggtcatcgc ttcaccgccg gccgcgtcgg caacctgcgc cgccactggg
     1981 gcattccttg cttcgagcaa acggccaatg cgccggaagg cgaactaatg acagtcaaga
     2041 aggctgctgt cgtgcttggc gtcgccccat ccactatcca tcgcttgctc aatgacggca
     2101 ttattgccgg tgagcagccg acacccggcg cgccttggcg tattcgcctc accaaagacc
     2161 tcaaggatcg tttccacggt gatgccggtg aggacttcct gccgatgcgt gaggcaatgc
     2221 gcgcgctcgg cgtctcgcgc cagaccgtat tgcagcgtgt caagcgcggc gaactcgccg
     2281 ccgtccatgt catgcgcggc caacaaaaag gcctgcgcat caaagttata ggccgacagc
     2341 ccgacctctt tcatcctatc tcatgaacca gggggcatta tgcagcacat tccaagaagc
     2401 ccttgaggtc gagatcgagc acccagtcgt aacgccaaca acgctcacga gccgtgcgca
     2461 cggcatcgat cgcagatttg ccggggcgat agccgtagga atcgggatgg aacaccggtt
     2521 ccaggatcgg ctccaagt
//
LOCUS       DATLKL010000689         1557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_19414, whole genome shotgun sequence.
ACCESSION   DATLKL010000689 DATLKL010000000
VERSION     DATLKL010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19414"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VM713_09315"
     CDS             complement(<1..376)
                     /locus_tag="VM713_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84501.1"
                     /translation="MLAFVLLSAALTVAGVAAVLIPLLRRGAAGAPAPWAALAAGTLL
                     FVGSAALYFSWSNWTWPGAAAEDSPQRMVGRLARRMEKDPGNLQGWLQLGNSYVTLQE
                     YHLALRAFGKADELSGGKNADAL"
     gene            complement(376..846)
                     /locus_tag="VM713_09320"
     CDS             complement(376..846)
                     /locus_tag="VM713_09320"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HVH84502.1"
                     /translation="MPHLMNRDLKRARLAACLLPLAALACVSTAGAVDPTQMPTPELQ
                     ARYQGLTHELRCVQCQDEALADSPAGIASDLRGEVRDMLLAGKSDDDVRNFMLARYGD
                     FVLFRPRFAWKNAWLWSAPEVLLIVGVLVAWRVVRKRARLVSADSEPLPDDGSP"
     gene            complement(857..1399)
                     /locus_tag="VM713_09325"
     CDS             complement(857..1399)
                     /locus_tag="VM713_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HVH84503.1"
                     /translation="MINRFIIPLVAFALLAVVLAYGIVHAPEKGVIASPLIGKPAPQF
                     SLRSLADGTRTVNTAALRGRWYLLNVWGTWCVTCREEHRMLLEVRRSGRVPLIGLDWK
                     DDDTTALSYLTQFGNPYEVVAVDHDGRTAIDWGVYAAPESFLVNPQGVVVYKCMGELT
                     PEVWEKEILPRLPKTQAATS"
     gene            complement(1396..1548)
                     /locus_tag="VM713_09330"
     CDS             complement(1396..1548)
                     /locus_tag="VM713_09330"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HVH84504.1"
                     /translation="MRIQIRPLVSFIWIAAVIMALGGAVAISDRRYARAKAAVAVPAE
                     GTPEHA"
BASE COUNT          251 a          547 c          533 g          226 t
ORIGIN      
        1 gcagcgcgtc ggcgttcttc cccccgctca gctcgtcggc cttcccgaag gcacgcagcg
       61 ccagatggta ttcctgcagc gtgacatagg agttcccgag ctgcagccag ccctggaggt
      121 tgccgggatc cttctccatg cgacgcgcca ggcgccccac catgcgctgc ggcgagtctt
      181 cggccgctgc ccctggccag gtccagttgc tccagctgaa gtaaagcgcc gccgagccga
      241 cgaacagcag cgtgcccgcg gcgagcgccg cccagggagc tggtgccccc gcggcgccgc
      301 ggcgcagcag cgggatcagt accgcggcca cacccgccac ggtaagtgcc gcggagagca
      361 gaacgaaggc gagcatcacg gcgacccgtc gtcgggcagc ggctcgctgt ccgcgctcac
      421 cagccgggcg cgctttctca ccacgcgcca cgccaccagc acgccgacga tgagcaacac
      481 ctcaggtgcg ctccacagcc aggcgttctt ccacgcgaag cgcgggcgga acagcacgaa
      541 atccccgtag cgcgcgagca tgaagttgcg cacgtcgtcg tcggacttgc cggcgagcag
      601 catatcgcgc acctcgcccc gcaggtcgga cgctatgccg gccggcgagt cggcgagcgc
      661 ctcgtcctgg cactgtacgc agcgcaactc gtgggtgagg ccctggtagc gcgcctgcag
      721 ctccggcgtc ggcatctgcg tcgggtcgac cgcgccggcg gtggagacgc aggcgagtgc
      781 ggcgagcggc agcaggcagg cggcgaggcg tgctcgtttg agatcgcggt tcatgaggtg
      841 cggcatgagg tcggtgtcac gaggtcgcgg cctgcgtctt gggcagccgg ggcaggattt
      901 ccttctccca gacttcgggc gtgagctcgc ccatgcattt gtacacgacg acgccctggg
      961 gattgaccag gaagctctcc ggcgccgcat acacgcccca gtcgatggcc gtgcggccgt
     1021 cgtgatcgac cgcgacgacc tcgtacggat tgccgaactg cgtcagatag gaaagcgccg
     1081 tggtgtcgtc gtccttccag tcgagcccga tcagtggcac ccggcccgag cggcgtacct
     1141 cgagcagcat gcggtgctcc tcacggcagg tgacgcacca ggtgccccac acgttgagca
     1201 ggtaccagcg tccgcgcagc gccgcggtgt tgaccgtgcg tgttccgtcc gcgaggctgc
     1261 gcagagagaa ctgcggcgcc ggcttgccga tcagcggcga ggcgatcacg cccttctcgg
     1321 gcgcatgcac gatgccgtag gcgagcacca ccgcaagcag cgcgaacgct accaacggga
     1381 tgatgaaacg gttgatcacg cgtgctccgg cgtgccctcc gcgggcacgg ctaccgccgc
     1441 cttggctctg gcgtagcgcc ggtcggagat cgccacggcg ccgcccaatg ccatgatgac
     1501 cgcggcgatc caaatgaagc tcaccagcgg gcggatctgg atgcgcacgc tccagcg
//
LOCUS       DATLKL010000690         2866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_53270, whole genome shotgun sequence.
ACCESSION   DATLKL010000690 DATLKL010000000
VERSION     DATLKL010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53270"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VM713_09335"
     CDS             complement(<1..563)
                     /locus_tag="VM713_09335"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HVH84505.1"
                     /translation="MAEFLELHPITPQPRLIRRAAEIVRAGGLIAYPTDSCYAFGWHL
                     GDKGPLERVRRIRQADRHHHFTMVCASLAQVGRFALIETWQFRILKACMPGPYTFLLR
                     ATRATPRRLQHERRRSIGVRIPDHAVPRLLVKELGEPLMSSTLMLASEPQPLTEAREI
                     SARLAHDVDAILDGGDCGVEPTTVVDLS"
     gene            661..1251
                     /locus_tag="VM713_09340"
     CDS             661..1251
                     /locus_tag="VM713_09340"
                     /inference="COORDINATES: protein motif:HMM:NF016189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HVH84506.1"
                     /translation="MQALTEFAPLLAFIVAYYLRGLYAATAVLMGAMLLLLAVDWLRA
                     RRIPPMHALSTVLVLIFGGATLALHDRAFIQWKPTVFFWGVSLAFLGSFWIGARTLAE
                     RLLGATLAEAFGERLRVSAPLWRRLNLLWAVFYALLGALNIVVLRYFSERAWVALKLV
                     DFVLMLLFLLAQVLWLAARAAPAGEQAIEGGDGHTE"
     gene            1235..1510
                     /locus_tag="VM713_09345"
     CDS             1235..1510
                     /locus_tag="VM713_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HVH84507.1"
                     /translation="MGTPNDVPARIRAALERAFAPSALEIIDEGAQHAGHREAGRGHF
                     RVLLVSDAFRGRSRLERHRMVYAALGPLLESSVHALAITARTAAEND"
     gene            1538..2479
                     /locus_tag="VM713_09350"
     CDS             1538..2479
                     /locus_tag="VM713_09350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVH84508.1"
                     /translation="MTLNLPRLLLAAALGLQLTACAPKASAPADTSPPVATVNGKPIS
                     RDLYEFYVKNVSGKTSAELTPDIRDKLLDNMVRGEVIAQEAVRQGLDKSGDTAYILEL
                     SRLNVLGQAVGDHYLKDKKPTEPELRADYDAYVAAAPKTEYHARHILVATEPFAEKVI
                     QRLGRGEKFEDLARVESMDPSKSNGGDLSWIRPESVPPEFMKALESLKPGEYTKTPVQ
                     TSFGWHIVQLVETRPLAPQAYEQRKPRIEQEIERKKFKDYIDELMRTAQVKKMLDQGA
                     PAAAALAPASPASPVSPAPVPPATPAPAPAPAAAPKN"
     gene            complement(2498..>2866)
                     /locus_tag="VM713_09355"
     CDS             complement(2498..>2866)
                     /locus_tag="VM713_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84509.1"
                     /translation="ARALREGPSSHLPPHLTAVLGLAGGSDTEGLAVRQLVFRDGFSV
                     HTFNVGAAQPHVVVLMVADEAANRHVAYLLTPRGQLRKAVAYHSGEAPQELTAKAARP
                     GFAAEVRFWSGAAGAPAAPR"
BASE COUNT          450 a          994 c          984 g          438 t
ORIGIN      
        1 gacagatcga cgacggtggt cggctcgacg ccgcagtccc ccccgtcgag aatcgcatcg
       61 acgtcgtgcg cgagacgtgc actgatctcg cgcgcttcgg tgaggggctg cggttccgag
      121 gccagcatca gcgtcgagct catgagtggc tcgcccagct ccttcaccag cagccgcggc
      181 accgcgtggt cggggatgcg cacgccgatg ctgcgccgcc gctcgtgctg caggcggcgc
      241 ggcgtcgcgc gcgtggcgcg taaaagaaag gtgtagggcc cgggcatgca ggccttgaga
      301 atccggaact gccaggtctc gatgagcgca aagcgcccga cctgggcgag acttgcgcac
      361 accatcgtga aatgatgatg acggtcggct tggcggatgc ggcgcacgcg ctcgagcgga
      421 cccttgtcgc ccagatgcca accgaacgcg tagcaggagt cggtcgggta ggcgatgaga
      481 ccgccggcgc gcacgatctc ggcggcgcgg cggatcaggc gcggctgcgg agtgatgggg
      541 tgcagctcga ggaactcggc catggcgggt cgcgtcggag ggctgttcgc cggggatcgc
      601 tcgtgacaat atacgcggcc gtttgccccg tacacccttg gtgtcgcatt ccgttcttcg
      661 atgcaggccc tgaccgagtt cgcgccgctc ctggcgttca tcgtggcgta ttacctacgc
      721 ggcttatacg cggccacggc agtcctgatg ggcgccatgc tgctgctgct cgcggtggac
      781 tggctgcgcg cgcgccgcat tccgccgatg cacgcgctgt cgacggtgct ggtgctgatc
      841 ttcggcggtg cgacgctcgc gctgcacgat cgcgcgttca tccagtggaa gccgacggtg
      901 tttttctggg gggtgagcct cgcgttcctc ggcagcttct ggatcggcgc gcgcacgctc
      961 gccgagcggc tgctcggcgc gacgcttgcg gaagccttcg gcgagcggct gcgcgtgagc
     1021 gcgccgctgt ggcggcgcct gaacctcctc tgggccgtgt tctacgcgct gcttggcgcg
     1081 ctcaacatcg tggtgctccg ctatttcagc gagcgcgcct gggtggcgct caaactggtc
     1141 gacttcgtgc tgatgctgct attcctgctg gcgcaggtgc tgtggctcgc cgcgcgggca
     1201 gcgcccgcgg gtgagcaggc catcgagggc ggcgatgggc acaccgaatg acgtgccggc
     1261 caggatacgc gccgcgctcg agcgcgcctt cgcgccgagc gctctcgaga tcatcgacga
     1321 aggcgcgcag cacgccggcc accgggaagc gggccgcggc cacttccgcg tgctgctggt
     1381 gtcagacgcc ttccgcggac gctcgcgcct cgagcgtcac cgcatggtgt acgccgcgct
     1441 cggccccctg ctcgagagct cggtgcatgc gctcgccatc acggcgcgca ccgccgcaga
     1501 gaacgactga cccgttacca caacctggat gcctcttatg accctgaacc tcccgcgcct
     1561 cctcctcgcc gcagctcttg ggctccagct cactgcctgc gcccctaagg cgagcgctcc
     1621 ggcggacact tcgccgccgg tggcgaccgt caacggcaag cccatcagtc gcgatctcta
     1681 tgagttctac gtcaagaacg tcagcggcaa gacttccgcg gagctcacgc ccgacatccg
     1741 cgataaactg ctcgacaaca tggtgcgcgg cgaggtgatc gcgcaggagg ccgtccgcca
     1801 aggcctcgac aagagcggcg acaccgccta catcctcgag ctctcgcgcc tcaacgtact
     1861 cggccaggcg gtgggcgatc actatctgaa ggacaagaag ccgaccgagc cggagttgcg
     1921 cgccgattac gacgcttacg tggccgcggc gcccaagacc gagtaccacg cccgccacat
     1981 cctggtagcc accgagccct tcgcggagaa ggtcatccag cggctcgggc gcggcgagaa
     2041 gttcgaagac ctcgccaggg tggagtccat ggatccctcc aagagcaacg gcggagacct
     2101 gagctggatc cggcccgagt ctgtgccccc ggagttcatg aaggcgctcg agtcgctgaa
     2161 gcccggcgag tacaccaaga cgccggtaca gacgtccttc ggctggcaca tcgtgcagct
     2221 ggtggagacg cggccgctgg ccccacaggc ttacgagcag cgcaagccgc gcatcgagca
     2281 ggagatcgag agaaagaagt tcaaggacta catcgacgag ctgatgcgca ccgcgcaggt
     2341 caagaagatg ctcgatcagg gcgccccggc cgccgccgcg ctagcgccgg cgagtccggc
     2401 ctccccggtg tcgcccgcac cggtcccgcc cgcgaccccg gctccggccc cagcgccggc
     2461 cgccgcgccg aagaactgaa gccgctcggc gggcgactca gcgcggagcg gcgggtgcgc
     2521 ccgccgcacc cgaccagaag cgcacctcgg cagcaaagcc cgggcgcgcg gccttcgctg
     2581 tgagctcctg cggcgcctcg ccgctgtgat acgcgaccgc cttgcgcagt tggccacggg
     2641 gcgtcagcag gtaggcgacg tgcctgttgg cagcttcatc ggcgaccatc agcacgacca
     2701 catgcggttg ggcggcgccc acattgaagg tgtgcaccga aaagccgtcg cgaaaaacca
     2761 gctggcgcac cgcgagcccc tcggtgtcgc tgccgccggc cagtccgagt accgcggtga
     2821 ggtgcggcgg caagtgcgag ctcggcccct cgcgcagcgc gcgcgc
//
LOCUS       DATLKL010000691         2688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_211247, whole genome shotgun sequence.
ACCESSION   DATLKL010000691 DATLKL010000000
VERSION     DATLKL010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211247"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VM713_09360"
     CDS             <1..589
                     /locus_tag="VM713_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84510.1"
                     /translation="FVRYIQPLLEKHFAVSGPEWDVENLYSHLTRVQRGVIRVDADEL
                     TYPLHIMLRYELEKQLLSGELAVRDLAEAWNAGMESRLGIRPANDLEGCLQDIHWAVG
                     SFGYFPSYALGAVIAAQLYESLRAEVTTLDEQLARGEFAGLFGWLRTQVHGLGAKVPV
                     QELLKSATGKPLSATSFVRYVEAKYLESATSVAAA"
     gene            638..1522
                     /gene="ttcA"
                     /locus_tag="VM713_09365"
     CDS             638..1522
                     /gene="ttcA"
                     /locus_tag="VM713_09365"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HVH84511.1"
                     /translation="MTRAAAKPSRTLERLTSRLRGAVGKAIADFRMIAPGDRVMVCLS
                     GGKDSYTLLDLLLSLQRSAPVHFELLAVNLDQKQPSFPSEVLPRYLESRGVPFHIIEQ
                     DTYSVVKRVVPEGRTLCGLCSRLRRGALYRFAATHGISKIALGHHRDDIVETLFLNLF
                     FGGRLKAMAPKLLSDDGRHVVIRPLAYVAERDIARFARGRAFPLIPCTLCGSQENLQR
                     LAVKKMLAAWEREFPGRTETIFRALRNVELEHLADPRRFDFAGLDVRRVAPMSEREER
                     SLEEQSLAGALASQPARA"
     gene            1530..>2688
                     /locus_tag="VM713_09370"
     CDS             1530..>2688
                     /locus_tag="VM713_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HVH84512.1"
                     /translation="MPDRLPLANCYWVVPGKLLAGEHPGGANLEATRERLRRLTAAGI
                     EVFLDLTQPGELVSYEAQLPEGAEYLRKPIPDHGLPERRGDMAGILESLRQVLDSPRS
                     VYLHCRAGIGRTGMVAGCFLAEQGRGGEAALAELNRLWQQSARSVQWPSIPETELQVE
                     YVRGWTAPPPPEGDPLLEPSTLAAARGLRERFLGALLGLALGDAAAAATQFKRPDRLA
                     PVGDLLGGGPFDLPRGGWSDDTAMALCLAESLLERNGFDARDQVERYTRWQRQGHLSA
                     TGRCLGITAGTARALARAQWRRQMFSGSHDPGAPDPEPLSRVTPVAMYFFGQPQQAQE
                     QAAQAARTTCQAPAVLSACSMLARALHAALAGQPRDAIVASAADAAAGAGAA"
BASE COUNT          377 a          950 c          944 g          417 t
ORIGIN      
        1 gttcgtgcgc tacatacagc cgctgctcga gaagcatttc gcggtcagcg gccccgagtg
       61 ggacgtagag aacctttata gccatctgac ccgcgtgcag cgcggcgtta tccgcgtgga
      121 cgcggacgag ctcacctacc cgctgcacat catgctgcgc tatgagctcg agaagcagct
      181 gctctcgggc gagctcgcgg tgcgggacct ggccgaggcg tggaatgccg gcatggagag
      241 ccgcctgggg atccggccgg cgaacgacct ggaggggtgt ctgcaggaca tacactgggc
      301 cgtgggctcc ttcggctact tcccctccta cgctcttggg gccgtgatcg ccgcgcagct
      361 ctacgagagc ctgcgggcgg aagtgacgac actcgatgag cagctcgcgc gcggcgagtt
      421 cgcgggactg ttcggctggc tgcgcaccca ggtgcatggc ctcggcgcca aggtgccggt
      481 gcaggagctc ctgaagagcg cgaccggcaa gccgctctcg gccacttcct tcgtccgcta
      541 cgtcgaggca aagtatctgg aaagcgctac gagcgtagcg gcggcctgaa gccgttgcgc
      601 cgcccgcggc gcgactcgct cgcaaacctg atccgacgtg acgcgcgccg ccgcaaagcc
      661 ctcccgcact ctcgagcgtc tcaccagccg cctgcgcggg gcggttggca aggcgatcgc
      721 cgactttcgc atgatcgcgc cgggcgaccg agtcatggtg tgtctctcgg gcggcaagga
      781 ctcctacact ctgctcgacc tgctgctgtc tctgcagcgc agcgcgccgg tgcatttcga
      841 gctcctcgcc gtcaatctcg accagaagca gccgagcttt ccgtccgagg tcctgccgcg
      901 ctacctcgag agccgggggg ttccgttcca catcatcgag caggacacct acagtgtggt
      961 gaagcgggtc gtgcccgagg ggcggaccct gtgcggactg tgctcgcggc tgcggcgcgg
     1021 ggcactgtac cgcttcgccg ccacgcacgg catcagcaag atcgcgctcg gccatcaccg
     1081 cgacgacatc gtcgagacgc tgttcctgaa tctgttcttc ggcgggcgcc tgaaggcgat
     1141 ggcgccgaag cttctgtcgg acgacggccg ccacgtcgtc atccgtccgc tcgcctatgt
     1201 tgccgagcgc gacatcgcac gtttcgcgcg cgggcgagcg ttccccctca ttccctgcac
     1261 gctgtgtggc tcgcaggaga acctgcagcg cctcgccgtg aagaaaatgc tcgccgcctg
     1321 ggagcgggag ttcccgggcc gcacggagac gattttccgt gcgctgcgca acgtcgagct
     1381 cgagcatctc gccgacccgc gccgcttcga cttcgccggc ctcgacgtgc gcagggtggc
     1441 gcccatgagc gagcgcgagg agaggagcct cgaggagcag agcctcgcgg gcgcactcgc
     1501 ttcgcagcct gcgcgcgcct gaggtgcgcg tgccggaccg actgccgctc gccaactgct
     1561 actgggtagt gccggggaag ctcctggccg gcgagcatcc cggcggcgcg aacctcgagg
     1621 cgacgcgcga gcggttgaga cgtctcacgg cggcgggcat cgaggtcttc ctcgatctca
     1681 cgcagcccgg ggagctcgtc tcctatgagg cgcagctgcc cgagggcgcc gagtatctcc
     1741 gcaagcccat tcccgatcac ggcttgcccg agcggcgcgg cgacatggct gggatcctcg
     1801 agtcgctgcg ccaggtgctc gactcgccgc gctccgtgta cctgcattgc cgggcgggca
     1861 tagggcgcac cgggatggtc gccgggtgct tcctcgcgga gcagggtcgc ggcggagagg
     1921 ccgccctcgc cgagctcaac cgtctctggc agcagagcgc gcgctcggtg cagtggccgt
     1981 ccatccccga gaccgagctg caagtcgaat acgtacgcgg ctggacggcg ccgccgccgc
     2041 cagagggcga tcctttactc gagccgtcga cactcgcagc cgcgcgcggc ctgcgcgagc
     2101 gctttctcgg cgcactcctc ggcctcgcct tgggggatgc cgctgccgcc gcgacgcagt
     2161 tcaagcgtcc agaccgcctt gccccggtgg gggacctcct cggcggcgga ccgttcgatc
     2221 tgccgcgggg cgggtggagc gacgacaccg cgatggcgct gtgtctcgcc gagagtctgc
     2281 tcgagcgcaa cggtttcgat gcccgcgacc aggtcgagcg ctacacccgc tggcagcggc
     2341 aggggcacct gtcggcgacg ggacggtgcc tcggcatcac ggccggcacg gcccgcgcgc
     2401 tcgcgcgggc gcagtggcgg cggcagatgt tctcggggtc gcacgatccg ggcgcgccgg
     2461 atcccgaacc tctgtcgcgg gtgaccccgg tggccatgta tttcttcggg cagccgcagc
     2521 aggcgcagga gcaggcggcc caggccgcac gcaccacctg ccaggcgccc gccgtgctca
     2581 gcgcctgcag catgctcgcc cgcgcgctgc acgcggccct tgcgggccag ccccgggacg
     2641 cgatcgtggc gagcgcggcc gacgcggcgg cgggcgccgg cgccgcct
//
LOCUS       DATLKL010000692         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_222257, whole genome shotgun sequence.
ACCESSION   DATLKL010000692 DATLKL010000000
VERSION     DATLKL010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222257"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /gene="hutH"
                     /locus_tag="VM713_09375"
     CDS             complement(<1..1408)
                     /gene="hutH"
                     /locus_tag="VM713_09375"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HVH84513.1"
                     /translation="MTETVLIAPGEFTLGQLRAIHRGRGRVALAEGCRSAVERAAATV
                     ADIVARGARVYGINTGFGSLAREPIPDDKLKALQQNLVLSHSAGTGPLLDDAAVRLIM
                     ALKVAALARGHSGVRWMVIEALLRLLEAEVHPCIPSKGSAGASGDLAPLAHLAAALIG
                     AGEVRHRGDTMPAGEGLKRAGLAPLELGPKEGLALLNGTQVSTALALVGLFAIEDAFA
                     AAVVAGAMSVDAAKGSDTPFDPRIHAIGGHPGQAQLAAVYRRMLAGSAIRASHFDCNR
                     VQDPYSLRCQPQVMGACLDQLRSAAEVLRREANAVSDNPLVFADSGEVLSGGNFHAEP
                     VALAADVLAIVVAEIGALSERRIALLVDHNMSELPPFLARESGLHSGFMIAQVTAAAL
                     ASENKTFAHPASVDSLPTSANQEDHVSMATFAARRLGEMADNSTGIVAIELLAAAQGI
                     EFHRPLQTSSILAEAKALI"
     gene            complement(1723..>2081)
                     /locus_tag="VM713_09380"
     CDS             complement(1723..>2081)
                     /locus_tag="VM713_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003829125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVH84514.1"
                     /translation="RDVPAGPFYGFNRPGAKPLEGVVENWWRQGMMGGAKAHYDGIKA
                     LSETDFTEDLKRVDVPTLVMHGDDDQIVPYKDAALLSVKLLKRGTLKIYPGFPHGMPT
                     TNADVINGDLLAFVRG"
BASE COUNT          336 a          700 c          737 g          308 t
ORIGIN      
        1 ggatcagcgc tttggcctcg gccaggatcg acgatgtctg cagtgggcgg tgaaactcga
       61 tgccctgggc cgcggccagc aactcgatcg ccacgatgcc cgtgctgttg tctgccatct
      121 cgccgagccg gcgcgccgcg aacgtggcca tcgagacgtg atcctcctga ttggccgagg
      181 tcggcagact gtcgacgctg gccggatggg cgaacgtctt gttctctgac gcgagcgccg
      241 ccgcggttac ctgggcgatc atgaatccgg aatgaagccc gctctcgcgc gccagaaacg
      301 gcggcagctc ggacatgttg tgatcgacga ggagcgcgat gcggcgctcg gagagcgcac
      361 cgatctcggc caccacgatg gcaagcacgt cggccgccag cgccaccggc tcggcgtgga
      421 aattcccgcc cgaaagcacc tcgccgctgt ccgcgaagac cagcgggttg tcggagaccg
      481 cgttggcctc gcgccgcagc acctctgcgg cggagcggag ctggtcgagg caggccccca
      541 tcacctgcgg ctggcagcgc agcgagtagg gatcctgcac gcgattgcaa tcgaaatgcg
      601 acgcccgaat ggcgctgccc gcgagcatcc ttcgatacac cgccgccaat tgggcctggc
      661 cgggatggcc gccgatggcg tggatgcggg ggtcgaacgg cgtgtccgag cccttcgccg
      721 catcgaccga catggcgccc gcaacgaccg cggcggcgaa ggcgtcctcg atggcgaaga
      781 gcccgacgag cgcgagggcc gttgagacct gagtgccgtt gagcagcgcc aggccctcct
      841 tggggcccag ctcgagcggg gcgagcccgg cccgcttgag accctcgcca gccggcatgg
      901 tgtcgcctcg atgccggacc tcgcccgcgc cgatgagggc cgcggcgagg tgggcgagcg
      961 gcgccaggtc gcccgaggcg ccggccgagc ccttgctcgg aatgcaggga tggacctcgg
     1021 cctcgaggag gcgcagcaaa gcctcgatca ccatccagcg cacgccggaa tgaccccgcg
     1081 ccagcgcggc gaccttgagc gccatgatca ggcgcaccgc ggcatcgtcc aggagcgggc
     1141 cggtcccggc gctgtgggag agcacgaggt tctgctgcag cgccttgagc ttgtcgtcgg
     1201 ggatcggctc gcgcgcgagc gagccgaagc cggtattgat cccgtagacc cgcgcgccgc
     1261 gcgcgacgat gtcggcaacc gtcgcggccg cgcgctcgac cgcagaccgg cagccttccg
     1321 caagcgcgac gcggccgcgg ccgcgatgga tggcgcgcag ctggccgaga gtgaactcgc
     1381 ctggtgcgat gaggacggtc tcggtcatgg gcggtgcgcg gccggatcgg agtgccgcgc
     1441 ggcgaaaagc gagagccagc ctggcgcggc ttgcgggctg tcacgcgata aagctcaagt
     1501 cgctcgctcc ctagaccgat aacattgcgt cggcccgcga acaaggcgca gcgacggtcg
     1561 ccctcgtgcc aatccgcaga cgtgcacagc acgcccgaag ccgtcctctg acaaaaagcc
     1621 gctatgtgct gaaggtcgcg cggagaatga cagcttcgaa cgcgcggcac cagcacggtt
     1681 gctacctgtt cgggcgcatc agggcgcccg gcccgctttg tgctagccgc gaacgaaggc
     1741 cagaaggtcg ccgttgatca cgtcggcgtt ggtggtgggc atgccgtgcg ggaagccggg
     1801 gtagatcttc agtgtgccac gcttgaggag cttgaccgag agcagagcgg catccttgta
     1861 cgggacgatc tggtcgtcgt ccccgtgcat cacgagcgtt ggtacgtcga ccctcttcag
     1921 gtcctcggtg aagtccgtct cggagagcgc cttgatgccg tcgtagtggg ccttggcccc
     1981 gcccatcatg ccttggcgcc accagttctc gaccacacct tccagcggct tcgcgcccgg
     2041 gcgattgaac ccgtagaagg gcccggccgg gacgtcgcga t
//
LOCUS       DATLKL010000693         2302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_30892, whole genome shotgun sequence.
ACCESSION   DATLKL010000693 DATLKL010000000
VERSION     DATLKL010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30892"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="VM713_09385"
     CDS             complement(<1..1565)
                     /locus_tag="VM713_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84515.1"
                     /translation="MARLFWLAIGLFSGLVAAVAAYGDSPVLSNADSETYRAAFAAAH
                     AEQWRAARSLAARAQDPLLAKVLQWYLLLRPEAGASFEEIASFISSNPDWPSLSALRE
                     RAEDTIPDRSSAGRVREWFERYPPVSTTGRIRYAEALIGTGEQAKGVEGLRKTWIEGN
                     FGERQEQIFLFRHFRQLRPEDHVARLDRLLWDGQTRAARSMLPHVDAGHAALAEARMH
                     LMRMAGGVDAALRRVPPGLANDPGLLLDRVRWYRAKGRDAEARAILMNPPVDLVQPDQ
                     WWEERSLQVRNLLASGHVADALRLAEEHGLTPRSASYADGEWLAGWIALRFLRDPERA
                     LQHFQVLDEVVRYPVTRARIAYWQGRAFEAMGNRQAAATLYAAAARFGMTYYGQLAAL
                     RLDSSARPRVQRPPQPQPFQREAFEQRELVRAVRALGAIGEDNLQEIFIKRIATLTTS
                     SPEEAELIADLVIEQVRSEFAVRLSRNTLHDAMPRPELGYPLRPLPDSTIPEAALVLA
                     VIRQESAFDAKAI"
     gene            1797..>2302
                     /gene="dapA"
                     /locus_tag="VM713_09390"
     CDS             1797..>2302
                     /gene="dapA"
                     /locus_tag="VM713_09390"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HVH84516.1"
                     /translation="MNMFNGSFTALITPMRDGEVDERAFQSFVDWQIKEGSHGVVPCG
                     TTGESPTLAHPEHMHVTALCIEVAKGRVPVIAGTGSNSTAEAIALAKHAEKAGADAQL
                     VVTPYYNKPTQEGLYRHYKAIHDSCGLPIIIYNIPGRSVIDMTVETMARLAELPRIVG
                     VKDATNDLT"
BASE COUNT          404 a          780 c          766 g          352 t
ORIGIN      
        1 ctgatcgcct tggcgtcgaa ggcgctctcc tggcggatca ccgcgagcac gagcgcggcc
       61 tcggggatgg tagagtccgg cagcgggcgc aatggatagc cgagctcggg tcggggcatg
      121 gcgtcgtgca gggtgttacg cgagagccgg acggcaaatt ccgaccgcac ctgctcaatc
      181 accagatcgg cgatcaattc tgcctcctcg ggcgagctgg tggtgagtgt cgcgatgcgc
      241 ttgatgaaga tttcctggag attatcttcg cctatggcac caagtgcgcg caccgcgcgc
      301 accagctcgc gctgctcgaa agcctcgcgt tgaaagggct gcggctgcgg cggacgctgc
      361 acgcgagggc gagccgacga gtccaggcgg agggcggcga gctggccgta ataggtcatg
      421 ccgaagcgcg cagcggcggc gtagagggtg gccgccgctt ggcgattgcc catggcctcg
      481 aaggcgcggc cctgccaata ggcaatgcgc gcccgggtca ccgggtagcg caccacttca
      541 tccaggacct gaaaatgctg gagcgcgcgc tcggggtcgc gcaggaaacg caatgcgatc
      601 cagcccgcga gccactcgcc gtccgcatag cttgcggatc gcggcgtcag gccatgctcc
      661 tcggcgaggc gaagcgcatc cgcgacatgg ccactggcaa gcaagttgcg gacctgcagc
      721 gagcgttcct cccaccactg gtccggttgc accaggtcga caggcgggtt catcaggatg
      781 gcgcgcgcct ctgcgtcacg gcccttggcc cgataccagc gcacgcggtc gaggagcagc
      841 ccgggatcgt tggcgaggcc gggtgggact cggcgaaggg ctgcatccac cccgcccgcc
      901 atgcgcatca ggtgcatacg agcctcggca agggcggcgt ggccggcgtc gacgtgggga
      961 agcatgctgc gcgcggcgcg cgtttgcccg tcccacaata agcggtcgag tcgagcaaca
     1021 tggtcctcgg ggcgcagctg ccggaagtgc ctgaagagga aaatctgctc ctggcgctcg
     1081 ccgaaattgc cctcgatcca ggtcttgcgc aggccctcga cccccttcgc ctgctcgccc
     1141 gtcccgatga gcgcctcggc gtagcgaatc cggccggtag tcgacacggg cgggtagcgc
     1201 tcgaaccact ccctcacgcg gccggccgag ctgcggtcgg ggatcgtatc ctccgcgcgc
     1261 tcacgcagcg cgctgagcga cggccagtcc ggattgcttg aaatgaagct agcgatttcc
     1321 tcgaagctcg cgcccgcctc cggccgcagc agcaggtacc actgaaggac cttggcgagc
     1381 agcgggtcct gtgctcgtgc cgcaagcgac cgggccgcgc gccactgctc cgcatgtgcc
     1441 gccgcgaagg cggcgcgata ggtctcgcta tcggcgttgg agaggacggg cgagtccccg
     1501 tacgccgcga cggctgcgac cagcccggaa aacaagccga tcgccagcca gaacagtctt
     1561 gccacccctc tctcgctcct tagcttgggt agcctcagga ttctatgacg ccatcccaac
     1621 agccccgacc caacgccccg gcggatagta ggcgcgcgcg gccaattcgc caaggtccga
     1681 cgcagccgca tgcgctgggg cgccgtctta ggacgcttgc ccggctcagg cgcgggcgct
     1741 atcgtccctc gcaacggggc gatggctcca tagcggcacc gccccagaaa ggataaatga
     1801 acatgtttaa cggatcgttt accgcgctga ttacgcccat gcgcgacggc gaggtcgacg
     1861 agcgtgcctt ccaatccttc gtcgactggc agatcaagga gggcagccac ggcgtcgtcc
     1921 cctgcggaac cacgggggaa tcgcccacct tggcccatcc ggagcacatg cacgtgaccg
     1981 cgctctgcat cgaggtggcc aaggggcggg tcccggtcat cgccggcacc ggctccaaca
     2041 gcaccgccga ggccatcgca ctggccaagc atgcggagaa ggcaggcgcc gatgcccagc
     2101 ttgtggtcac cccctactac aacaagccga cccaggaggg gctctaccgc cactacaagg
     2161 cgattcacga ttcttgcggc ttgccgatca tcatctataa cattcccggc cgctcggtaa
     2221 tcgacatgac ggtcgagacc atggcgcggc tcgccgagct gccgcggatc gtcggcgtca
     2281 aggatgcgac caacgacctg ac
//
LOCUS       DATLKL010000694         1995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_30900, whole genome shotgun sequence.
ACCESSION   DATLKL010000694 DATLKL010000000
VERSION     DATLKL010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30900"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /gene="xrtD"
                     /locus_tag="VM713_09395"
     CDS             <1..1263
                     /gene="xrtD"
                     /locus_tag="VM713_09395"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM-specific exosortase XrtD"
                     /protein_id="HVH84517.1"
                     /translation="LVIVLLGTALALLGWQAFRIALVPLALLFLMNPIPNFFYYNLSA
                     ELQLISSELGVALMRFCGVSVFLEGNVIDLGSYQLQVAEACSGLRYLFPLMTLGVIIA
                     YLLRGPAWMRWLVFLSTVPITVLMNSFRIGVIGLLVDRYGVAQAEGFLHLFEGWVVFM
                     ICLLLLTGETWVLARLAGDRRALVDLLGFEPPPPPAASPITRNRELGKPAGALLIILL
                     VAVWPARALPKRVEVQPARANFGELPLQIGPWHGHRQSIETVYLDTLKLDDYVLADFV
                     RDAPGPPVNLYVAYYASQRTGEAAHSPRSCLPGGGWRILDFTQHEVAGVRFNRAPLRV
                     NRAIVQHGADRQLVYYWFQERGRDITNEYLVKWYLLEDALLRNRTDGALVRLITPMPE
                     TESAAAGDARLAQFVSSVLPELHEYLPN"
     gene            1266..>1995
                     /locus_tag="VM713_09400"
     CDS             1266..>1995
                     /locus_tag="VM713_09400"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HVH84518.1"
                     /translation="MNPFLQLTLAAAVSMLIIPLARHFAPRLGLVDMPDPRKVHTVPI
                     PRVGGWGITLGILLPLVLVFRQDLLVRYLLFGYLTLFAFGVWDDARTIGHWAKFAGQL
                     LAAVLVVYFGGLWVSRVPFIDAVIPAALGKPFTVFALVGVINAINHSDGLDGLAAGES
                     LMSLTALAILGYLSGSTLVLALSLTAIGGIVGFLRYNSHPAYVFMGDSGSQVLGFTLG
                     FLAVYLTQVADTNVSAALPLLLVGL"
BASE COUNT          285 a          652 c          630 g          428 t
ORIGIN      
        1 ctcgtcatcg tactgctggg aactgcgctc gccctgttgg gctggcaagc cttcaggatt
       61 gcgctggtac cgctcgcgct gctgtttctg atgaatccga ttcccaattt cttctactac
      121 aacctctctg ccgagctgca gctcatctct tccgagctcg gcgtggcgct catgcgtttc
      181 tgtggtgtca gcgtgttcct ggagggcaat gtgatcgacc tcggcagcta ccagctgcag
      241 gtcgcggagg cctgtagcgg gctgcgttac ctctttccgc tgatgacgct cggcgtgatc
      301 atcgcgtatc tgctccgggg ccccgcctgg atgcgctggc tcgtgtttct ctcgaccgtt
      361 ccgatcaccg tgctcatgaa cagcttccgg atcggagtga tcggcttgct cgttgaccgt
      421 tacggagtgg cgcaggccga gggctttctg catctgttcg aggggtgggt ggtcttcatg
      481 atctgcctcc tcctgctcac cggagagacc tgggtgctgg cccgcctcgc gggcgatcgg
      541 cgcgccctcg tggacctgct cggcttcgag cctccgccgc ctccggctgc gagccctatc
      601 acccgcaacc gcgagttggg caagcccgcg ggcgcactgc taatcattct gctcgtggcg
      661 gtctggcccg cgcgcgcgct gccgaagcgc gtcgaagtac aacctgcgcg cgcgaacttt
      721 ggtgagctgc cgttgcagat cggcccctgg cacggtcacc gccagtcgat cgagacggtt
      781 tatctggata cgctgaagct cgacgattat gtcctcgccg acttcgtgag agatgcccca
      841 ggcccgccgg tgaatctcta cgtcgcctat tacgcctcgc agcgcacggg cgaggccgca
      901 cattcgccgc gctcgtgcct accagggggc gggtggcgca ttctcgattt tacgcagcac
      961 gaggtggcgg gggtgcgctt caaccgcgca ccactgcgag tcaatcgcgc gatcgtgcag
     1021 cacggcgcgg atcggcagct cgtctattac tggtttcagg agcggggacg cgacatcacc
     1081 aacgagtatc tggtgaagtg gtacctgctg gaggacgcgt tgctgcgcaa ccgcaccgac
     1141 ggcgcgctcg ttcggctcat cacaccgatg ccggaaaccg agtccgcggc ggcgggggat
     1201 gcgcggcttg cgcaattcgt atcgagcgtg ctgccggaac tgcacgagta tctgccaaac
     1261 tgagcatgaa tcccttcctc cagctgacgc tcgcagccgc agtcagcatg ctgatcattc
     1321 cattggcgcg acatttcgcc ccacggctcg ggctggtgga catgcccgat ccccgcaaag
     1381 tgcataccgt gccgatcccg cgcgtcggcg gctggggcat cacgctcggg atcctgctgc
     1441 cgctcgtcct ggtattccgc caggacctgc tggtgcggta tctgctgttc ggctatctga
     1501 cgctcttcgc gttcggcgtc tgggacgacg cccgcacgat cggccactgg gcgaagtttg
     1561 cgggccagct gctggccgcg gtgctggtcg tctatttcgg cggcctatgg gtatcgcgtg
     1621 taccgtttat cgacgccgtc attccggcgg cgctcggcaa gcccttcacg gtattcgcgc
     1681 tcgtcggcgt gatcaacgcc atcaaccact ccgacggact cgacgggctg gccgccggcg
     1741 aatcgctgat gagcctgacg gcgcttgcaa tcctcggtta cctctcgggc agtacgctcg
     1801 tgctcgctct gtcgctcacg gcgatcgggg gcatcgttgg gtttctgcgc tacaacagtc
     1861 atccggcgta tgtattcatg ggcgacagcg gcagccaggt gctgggattc acactcggct
     1921 tcctagccgt ctacctcaca caggtcgcgg acaccaatgt gagcgcggcg ctgccgctgc
     1981 tgctcgtcgg gctgc
//
LOCUS       DATLKL010000695         1724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_42233, whole genome shotgun sequence.
ACCESSION   DATLKL010000695 DATLKL010000000
VERSION     DATLKL010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42233"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..>1724)
                     /locus_tag="VM713_09405"
     CDS             complement(26..>1724)
                     /locus_tag="VM713_09405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVH84519.1"
                     /translation="SSHTVGACLVGAPIVLFSAERWKRLLRRRYRLENALTLLLILLG
                     TYLLTRYTRFPFVTIGALLFIGAFRLEALGAALAGLATGLLIVGLWLFGIQPAGLEST
                     AAAGTLIGLPALALLAAVMPPVTVGIGAAARRLTLRALTMSERRFRESIAHSPIGMLI
                     AELDGTWSYSNVALQKMLGYTAEELRSLPPGGPSSQDEWEATEARRQKLISGETAVYE
                     VDRRFRHKDGHWVWTHVAVSLLRNEDGSPQHLLAQIESLEARLRAEAAVAEERERLRV
                     TLNSISDAVVTLDAEMRIDFINESAQHLLGFDADAARLRRIDEVIHLTDPHTSKSVVS
                     LATLAGVHGKVMRRDSPCILHRPDGVTCYVSDSASPLLDTAGHVTGIVLALRDATADV
                     ERTRELRHRATYDPLTGLVNRAEFQQRLRDAFRKARHLDRPAALLAIDLDRFKALNDE
                     AGHAAGDAMLRQVAEACRVHVRSSDTVARLGGDEFAIILDNCAGKFVDSISQKILHTL
                     NPLEIEWEGARRTIGASIGVSTVTGEMVSESDWMEAADKACYEAKSHGRGQIRVAPV"
BASE COUNT          265 a          573 c          605 g          281 t
ORIGIN      
        1 ggtagccgcc cgcgcggcgc ggctttcaga cgggtgctac ccggatctgc ccgcggccgt
       61 gtgatttcgc ctcatagcag gccttgtcgg cggcttccat ccagtcgctc tcactcacca
      121 tctctcccgt caccgtcgag accccgatgc tcgcaccgat ggtgcgccgg gctccctccc
      181 actcgatctc caggggattg agggtgtgca ggatcttctg cgagatgctg tcgacgaatt
      241 tcccggcgca attgtcgagg atgatggcga actcgtcgcc gccgaggcgg gccaccgtgt
      301 ccgatgagcg cacgtgcact cggcaggcct cggcgacctg gcgcagcatc gcatcgccgg
      361 cggcgtgacc tgcctcgtcg ttcagcgcct tgaagcggtc gaggtcgatc gccagcagtg
      421 cagcgggacg gtccaggtgg cgtgccttgc ggaacgcgtc ccgcaggcgt tgctggaact
      481 cggctcgatt caccagtccg gtgagcgggt cgtaggtcgc gcggtggcgc agctcgcgcg
      541 tgcgctcgac gtcggcggtg gcgtcgcgga gcgccaggac gattcccgta acatgtccgg
      601 cggtatcgag cagcggcgag gcgctgtcgc tgacgtagca ggtcacgcca tcgggccggt
      661 gcaggatgca cgggctgtcg cgccgcatca ccttgccgtg cacgccggcg agagtcgcga
      721 ggctcacgac gctcttcgag gtgtgcggat cggtgaggtg gatcacctcg tcgatgcgcc
      781 gcagccgcgc cgcgtcggca tcgaatccca gcaggtgctg ggccgactcg ttgatgaagt
      841 cgatgcgcat ttcggcatcg agggtgacga ccgcgtcgct gatcgagttc agcgtcaccc
      901 gcagccgctc gcgctcctcg gcgaccgcgg cctccgcgcg caggcgcgcc tcgaggctct
      961 cgatctgcgc gagcaggtgc tgtggcgagc catcctcgtt gcgcagcagc gatacggcga
     1021 cgtgcgtcca cacccagtgg ccatccttgt ggcggaagcg gcggtccact tcgtagacgg
     1081 ccgtttctcc gctgatcagc ttctgacgac gcgcctcggt cgcttcccat tcgtcctggc
     1141 tggagggccc gccaggtgga agcgagcgca actcctcggc ggtgtatccc aacatcttct
     1201 gcagggcgac attactgtag ctccaggtgc cgtccagctc ggcgatcagc atgccgatcg
     1261 gagagtgcgc gatcgattca cggaagcgcc gctcgctcat cgtgagtgcg cgcagcgtca
     1321 ggcgacgcgc ggcagcgccg atgccgacgg tcaccggcgg catcactgcc gcaagcagcg
     1381 cgagcgccgg caggccgatc agcgttccgg cggcggccgt gctctcgagg ccggcgggct
     1441 gaatgccgaa gagccagagg cccacgatca ggagccccgt ggcgaggccg gcgagcgcgg
     1501 cgccgagagc ctccagcctg aatgcgccga tgaagagcaa cgccccgatg gtgacgaagg
     1561 gaaaacgggt atagcgggtc aggaggtacg tgccgagcag aatgaggagg agcgtcagtg
     1621 cgttctccag ccgatagcgc cggcgcagca gcctcttcca gcgctcggcg ctgaagagca
     1681 cgatcggcgc gccgacgagg cacgcgccga ccgtatgcga tgac
//
LOCUS       DATLKL010000696         1623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_87313, whole genome shotgun sequence.
ACCESSION   DATLKL010000696 DATLKL010000000
VERSION     DATLKL010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87313"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(418..888)
                     /gene="rnhA"
                     /locus_tag="VM713_09410"
     CDS             complement(418..888)
                     /gene="rnhA"
                     /locus_tag="VM713_09410"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003180633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HVH84520.1"
                     /translation="MTARGRGEEAVVDVFTDGACSGNPGPGGWGVLMRWQGRERELKG
                     GEPETTNNRMELMAGIRGLEALTRASRVRLHTDSLYLREGITSWIKAWKANGWRTADR
                     KPVKNADLWRRLDAALAGHEVSWHWVRGHAGHAENERADRLAREGMKPYLPKKD"
     gene            complement(885..>1623)
                     /locus_tag="VM713_09415"
     CDS             complement(885..>1623)
                     /locus_tag="VM713_09415"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF003558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HVH84521.1"
                     /translation="GIACPTPVHGRDGQALRELCGRPAAIVSFLKGMWARRITPRHCA
                     LLGEAMARLHLAGLSFDMKRRNALGPEGWRPLFEASSTRADELRRGLAAELEAELVHL
                     EAHWPRDLTHGVIHADLFPDNVFFLGDALSGIIDFYFACTDALAYDLAICLNAWCFES
                     DGSFNVTKARYMLSSYRKVRPFTAAELAALPLLARGSALRFLLTRLFDWLNHSPGAFV
                     KPKDPLEYLEKLRFHRGIARPAAYGLE"
BASE COUNT          256 a          540 c          570 g          257 t
ORIGIN      
        1 atattcgtgt gcgaggcgat cgaggccggc aggtgttccg ggctcggcgg cgagaaggac
       61 ggcaagcacc gcggcccggt ggccgtcacc cccatcacgc catccgcggc aaaagacgcg
      121 ttcgccgttc tcccacaacg gttggagggc attctctcca ccatctagcg atgagttcat
      181 ctgccaagct ccgccatcag gccgggggca gtcactgaga gatactacat taagacattc
      241 aagaggatgg cgagagcgga ttcggaggtg gaatttgtca cgcgcgagac ggcgtgacaa
      301 tgtccgttag tggcacgagt cggacctccg gcgatgtcgg cattcgcgcc gctgtcaggg
      361 gcatagcgga catcgaacgc gcctgacccg agctgccccg atttatgagt gcacggccta
      421 atccttcttc ggcaggtacg gcttcattcc ctcgcgggcg aggcggtcgg cgcgctcgtt
      481 ctccgcgtgg ccggcgtggc ccctgaccca gtgccaggag acctcgtggc ccgccagtgc
      541 cgcatcgagc cgccgccaca ggtcggcgtt cttgaccggc ttccggtccg ccgtgcgcca
      601 gccgttcgcc ttccaggcct tgatccagct ggtgatgccc tcgcggagat agaggctgtc
      661 ggtgtggagg cgcacgcggc tcgcgcgcgt cagcgcctcg aggccgcgga tcccggccat
      721 cagctccatg cggttgttgg tggtctcggg ctcgccgccc ttgagctcgc gctcgcggcc
      781 ctgccagcgc atcagcacgc cccagccgcc cgggccggga ttcccgctgc aggcgccgtc
      841 ggtgaagacg tcgacgacgg cctcctcgcc tcgcccgcgc gccgtcattc gagcccgtag
      901 gcggcggggc gcgcgatgcc ccgatggaag cgcagcttct cgagatactc gagcggatcc
      961 ttgggcttca cgaaggcgcc gggcgaatgg ttgagccagt cgaagaggcg cgtcaggaga
     1021 aagcgcagcg cgctgccgcg cgcaagtagg ggtagggccg cgagctcggc ggccgtgaat
     1081 ggccgcacct tccggtagct cgagagcatg tagcgcgcct tggtcacatt gaagctcccg
     1141 tcgctctcga agcaccaggc attgaggcag atcgccaggt cgtaggccag cgcgtcggtg
     1201 caggcgaagt agaagtcgat gatgcccgag agcgcgtcgc cgaggaagaa gacgttgtcg
     1261 ggaaagaggt cggcgtggat gacgccatgg gtgaggtcgc gcggccaatg ggcctcgaga
     1321 tgaacgagct cggcctccag ctccgcggcg agtccgcgac gcagctcgtc cgcccgggtg
     1381 ctgctcgctt cgaacagggg ccgccagccc tccggcccga gcgcgttgcg ccgtttcatg
     1441 tcgaaggaga gcccggccag gtgcagccgg gccatcgcct cgcccaaaag cgcgcaatgg
     1501 cgcggggtga tgcggcgcgc ccacatgccc ttgaggaagc tcacgatcgc ggcgggccgg
     1561 ccgcacagct ccctgagcgc ctgtccgtcg cgcccgtgca ccggcgtggg gcaggcgatg
     1621 ccc
//
LOCUS       DATLKL010000697         2716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_121215, whole genome shotgun sequence.
ACCESSION   DATLKL010000697 DATLKL010000000
VERSION     DATLKL010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121215"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VM713_09420"
     CDS             complement(<1..699)
                     /locus_tag="VM713_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVH84522.1"
                     /translation="MVRVLVAAGSALTAVTAGAADPADAKRAVVEVGTVSLKPVTVVS
                     WIPGSIVSRSDARVATVIPGRVTWIAEVGTRVRQGDPIARLDDTVSRLRLEDLAAQVA
                     RARAQHDVASSQLERFNRLAATQVLSASQLEDARAQRDVSGDDVTRAGAQLRQAQYEI
                     DQSVIRAPFPGVVSERFIQRGEYLQTGAATVRLVNTGDIEARATASLDLAANVHAGQA
                     VTVRDHGIEKSGKVR"
     gene            complement(736..>2716)
                     /locus_tag="VM713_09425"
     CDS             complement(736..>2716)
                     /locus_tag="VM713_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH84523.1"
                     /translation="TIPVCLCATFVTLDLFGRSLNVISLAGLAFAVGMVVEGAIVVSG
                     NVVRLRERGMPLEEACRKGAQQVAGALVASTATTIAVFVPVLFLKDVEGQLFGDLALT
                     ISIAVAISIVAALTLLPVALSFALNRPLPPSGYGQGWPRLTEWVLRVTATRPRQLAWI
                     GALLIAPLMLAWWMLPPLDYLPPVKRAAIDSFFDFPPGMSPEAVNRELLPTLLARMRP
                     YMEGKLEPRLKNWYIETWPGGGTIGARVIDERRIGELETLVRDKITAGLPDTRVFTVE
                     GDLFGGIDGSARSVGIHLQSEDTAALNRVALEGRTLLEKVFPNAPIQSFPNPEDVALE
                     LHAAPDDRRIAEVGWDRATLGTVVRTVGEGAWLGEYFDGKSRLPIILRSSEGATPEQL
                     TNAPLMTPAGLVVRLGDLVQLSTALGPPAVRRLNHRRVVTLTIDPPATLSLEKVLHTI
                     DTQVLPQLKAHLPADGSLRLAGSADSLSETLSTMSRVFALALVVLLLLMTVLFRSLRD
                     SMIVLLTVPLALVGGVVGLRLLDLVKFQALDLLSMIGFVMMIGVIINHAILLVAAVRS
                     VEREGAPLEDSIRAGLQQRLRAILASTLTGALGALPMALNPGPGSVIYRGLAAVNIGG
                     VVISLAFSLVLIPALMRLLARERRVAPAPVPAAVA"
BASE COUNT          437 a          948 c          950 g          381 t
ORIGIN      
        1 acgcactttg cccgacttct cgatcccgtg atcacggacg gtgacggcct gcccggcgtg
       61 gacattggcg gcgaggtcga gcgatgcggt ggcgcgggcc tcgatgtctc cggtgttgac
      121 gaggcgcaca gtggccgcac cggtctgcag atactcgccg cgctggatga agcgttcgct
      181 cacgactccc gggaacggag cgcggatcac cgactggtcg atctcgtact gcgcctggcg
      241 cagctgagcg ccggcgcgcg tcacatcgtc gccggacaca tcgcgctggg cgcgcgcatc
      301 ctcgagctga ctcgcgctca gtacctgcgt agcggccagg cgattgaagc gttcgagctg
      361 tgagctcgcc acatcgtgtt gggcgcgcgc ccgcgccacc tgcgccgcca gatcctcgag
      421 ccgcagccgc gacaccgtgt cgtcgagccg cgcgatggga tcgccctgcc ggacgcgggt
      481 gccgacctcc gcaatccagg taacccggcc cgggatcacc gtcgcgacgc gggcatccga
      541 gcggctgacg atcgatcccg ggatccagct caccaccgtc acgggcttga ggctcacggt
      601 accgacctcg accaccgccc gcttcgcgtc ggcgggatcg gcggccccgg ccgtcacggc
      661 ggtgagggcc gagccggcag cgaccagtac gcgaaccatc gtggcttgag tgcgactcat
      721 catatgctgg tgacctcagg cgacggcggc gggcaccggg gccggcgcca cgcgtcgctc
      781 gcgcgccagc agccgcatca gcgccgggat cagcaccagg ctgaaagcga ggctgatcac
      841 cacgccgccg atgttgaccg cggccagccc acgatagatg acgctgcccg gccccgggtt
      901 gagcgccatc ggcagcgcgc cgagcgcacc ggtgagggtg ctcgccagaa tcgcccgcag
      961 gcgctgctgc aatccggcgc ggatgctgtc ctcgagcggc gcgccctcac gctcgacgct
     1021 gcgtaccgcc gccaccagca ggatggcgtg gttgatgatg accccgatca tcatcacgaa
     1081 gccgatcatc gacagcaggt cgagcgcctg gaacttgacc aggtcgagga gccgcaggcc
     1141 gacgacgccg ccgaccaggg cgagcggcac cgtcagcagc acgatcatgc tgtcgcgcag
     1201 tgagcggaac agcacggtca tcagcagcag cagcaccacc agcgccagcg cgaacacgcg
     1261 gctcatggtg ctgagcgtct cgctcaggct gtcggcgctg ccggcgagcc gcagcgagcc
     1321 gtccgccggc aggtgcgcct tgagttgcgg cagtacctgc gtgtcaatcg tgtgcagcac
     1381 cttttccagc gacagcgtcg cgggaggatc gatggtgagc gtcaccacgc ggcggtgatt
     1441 gaggcggcgg accgcgggcg ggccgagcgc ggtcgacagc tgcacgaggt ccccgagccg
     1501 caccaccagc cccgcgggcg tcatgagcgg tgcattcgtg agctgctcag gggtggcgcc
     1561 ttcgctcgag cgcaggatga tcggcaggcg cgatttgccg tcgaagtact cgccgagcca
     1621 cgcgccttcg cccacggtgc gcaccaccgt gccgagcgtg gcgcgatccc agccgacttc
     1681 ggcgatgcgc cggtcgtcgg gcgcggcgtg cagctcaagg gcgacgtcct cgggattcgg
     1741 gaagctctgg atgggcgcgt tcggaaacac tttctcgagc agcgtgcgcc cctcgagggc
     1801 cacgcggttg agtgccgccg tgtcctcgct ctgcaggtgt atccccaccg agcgtgccga
     1861 gccgtcgatg ccgccgaaca ggtcgccctc gaccgtgaac acccgcgtgt cgggcaggcc
     1921 cgcggtgatc ttgtcccgca ccagggtctc cagctcgccg atgcgccgct cgtcgatcac
     1981 gcgcgcgccg atcgtgccgc ccccgggcca ggtttcgatg taccagttct tgagacgcgg
     2041 ctcgagcttg ccctccatgt aggggcgcat gcgcgcgagc agggtcggga gcaattcgcg
     2101 gttcacggcc tcaggactca tgcccggagg gaagtcgaag aatgagtcga tggccgcccg
     2161 cttgaccggc ggcaggtagt cgagcggcgg cagcatccac cacgccagca tcagcggcgc
     2221 gatcagcagc gccccgatcc aggcgagctg cctcgggcgc gtggcggtca cccgcagaac
     2281 ccactcggtg agcctgggcc acccctgccc gtagccgctc ggcggcagcg gacgattgag
     2341 agcgaaactc agtgccaccg gcagcagggt aagcgccgcg acgatcgaga tcgcgaccgc
     2401 gatggagatc gtaagcgcca ggtcgccgaa cagctggccc tcgacgtcct tgaggaacag
     2461 caccggcacg aacaccgcga tggtggtcgc ggtcgaggcg acgagtgctc ccgcgacctg
     2521 ctgcgctccc ttgcggcagg cctcctcgag cggcatgccg cgctcgcgca ggcgcaccac
     2581 gttgcccgag acgacgatcg ctccttcgac caccatgccg accgcgaagg cgaggccggc
     2641 gagggagatg acgttgaggc tgcggccaaa gaggtcgagc gtgacgaagg tggcgcacag
     2701 gcacaccggg atggtg
//
LOCUS       DATLKL010000698         2750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_143844, whole genome shotgun sequence.
ACCESSION   DATLKL010000698 DATLKL010000000
VERSION     DATLKL010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143844"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..708
                     /locus_tag="VM713_09430"
     CDS             43..708
                     /locus_tag="VM713_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84524.1"
                     /translation="MTRQGFAAATMVVLAAGIAGAALASAEISTVMGSINVSAGEHRG
                     DLSTVNGSIHVGENAVVGHAHSVNGSATLEAHATAAEVKTVNGSVRLNEGAEVNGRVQ
                     TVNGALSLAKGAAVTGALENVNGSIRVNAAHVGGDVETVTGNIEIGPDAHVDGGVIVR
                     ESHPSHFDSRKPQRIVIGPGSVVTGTLKFERPVELYVSDRATIGPVQGATPIRFSGDR
                     PPG"
     gene            complement(950..1711)
                     /locus_tag="VM713_09435"
     CDS             complement(950..1711)
                     /locus_tag="VM713_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84525.1"
                     /translation="MKTTHLFASAAIALLSVAALPAHAQLLGGGIRGGAAGAFGGPFG
                     GAQASEFERAHAAARTGATTRETAAGKAAAAGAVKHGADAGTRAGARAAADGRTGADE
                     AAMRGRGVLNAGASEGRAATQAAATQTAVSQGSDTFGNLAAGNNRVATGAAESASGGG
                     VTTLGEAGNQPSAGKPSSSGKPATNGNGKPSSDGKPSTTKPTSSGNPAPKPNGPSAGA
                     GFGGSAAGNASATPSGASVEASGNASATTSVNSSH"
     gene            complement(1817..>2750)
                     /locus_tag="VM713_09440"
     CDS             complement(1817..>2750)
                     /locus_tag="VM713_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVH84526.1"
                     /translation="APDDTARSLARLRGLDSAGSYDFNHVYLPSGAPADAADPVPSLP
                     VISAGAAPHPAAVRVGLIDDGVDGRHAALSAVTIHSHGCAGTALPAVHGTAVGSLLAG
                     RAGSFRGAAPGAELYAADVFCGRAAGGAVDEVAEAFAWLIRERVAVINVSLVGPPNRL
                     LESVVAGAVARGYLIVAAVGNDGPAAPPLYPAAWPGVVGVTAVDARQRVLAEAERGPQ
                     VRFAAPGADMTAARTPRGYALVRGTSFAAPIVAGLLAQALHSPDRAAADAALAALEQQ
                     AQHPGGGRRLDPIYGYGVVGAELAPPLALAARSE"
BASE COUNT          426 a          999 c          947 g          378 t
ORIGIN      
        1 catctatggg caggagcgtc accgccaacc ggagggaatc atatgactcg gcaaggcttc
       61 gccgccgcca ccatggtcgt gctcgcagcc ggcatcgccg gcgctgccct ggcgagcgct
      121 gagatctcga cggtgatggg ctcgatcaac gtgagcgccg gcgagcatcg cggcgacctc
      181 tccaccgtca acggctcgat tcacgtcggt gagaacgccg tcgtcggcca cgcgcatagc
      241 gtcaacggca gcgccaccct cgaggcgcac gcgaccgccg ccgaggtgaa aaccgtcaac
      301 ggctcagtgc ggctgaacga aggagccgag gtcaacgggc gcgtgcagac cgtcaacggc
      361 gcactgtcgc tcgccaaagg cgccgccgtc accggggcgc tcgagaacgt gaacggcagc
      421 atccgcgtca acgccgccca cgtcgggggc gacgtggaga cggtcaccgg caacatcgag
      481 atcggaccgg acgcgcacgt ggacggcggc gtgatcgtcc gcgagagcca tccgagccac
      541 ttcgattcac gcaagccgca gcgcatcgtc atcggcccgg gctcggtggt caccggcact
      601 ctgaagttcg agcggccggt ggagctctac gtgagcgacc gggcgacgat cggaccggtg
      661 cagggtgcga cgccgatcag gttctcgggc gaccgcccgc ccgggtgaag cgagcccggt
      721 cggtcggccg ccggcgcacg cgcagtccct cagcgcgata gttaggcacc ctgcctaact
      781 attgctagag cgaccttcac gcgctccggc cggtcgagcc ccgccttttg ccggcggcaa
      841 cgaagctgat gtccgacaga attgccgaag gcaattccgt ccaataaaaa aagagcggcg
      901 ggacataaag ggtcccgccg ctcagcgccc gtcaagaggc gcttcaacgt tagtgggaag
      961 aattgaccga agtcgtcgcc gacgcattgc ccgacgcttc aaccgacgca ccgctcggcg
     1021 tcgcgctcgc gttgccggcg gccgacccgc caaagcccgc accggcgctt gggccattcg
     1081 gcttgggcgc aggattgccg ctcgatgtcg gcttggtcgt cgagggcttg ccgtcagagg
     1141 aaggcttgcc gttcccgttc gtggcaggct tgccgctgct cgagggcttg cctgcagacg
     1201 gctggttgcc ggcctcgcca agggtcgtca cgcccccgcc gctggcggac tcagcagcgc
     1261 ccgtggcgac acggttgttg cccgcggcca gattcccgaa ggtgtcggag ccctggctca
     1321 cggccgtttg ggtcgcggct gcctgagtcg cggcgcgccc ttcgctcgcc ccagcattca
     1381 ggacgccgcg tccccgcatc gccgcctcgt ctgctccggt ccggccgtcg gccgccgcac
     1441 gcgcgccggc gcgtgtaccg gcatcggcac cgtgtttcac ggcacccgcg gcagctgcct
     1501 tgccggcagc agtctcgcgc gtcgtcgcgc ccgtgcgggc agcggcatgc gcacgctcga
     1561 actcgctcgc ctgagccccg ccgaaggggc cgccgaaggc cccggctgcg ccgccgcgaa
     1621 tcccaccgcc cagcaactgg gcgtgcgccg gcagcgctgc aacggacagc agggcaatcg
     1681 cggcgctcgc gaaaagatga gtcgttttca tggtcgtctc ctctcgaggc cgcggtccat
     1741 ccgcgacctt tcatcgagta ctacgaccgg gggccgccga ttcttcccct cggcggatgc
     1801 aagtgccggg ttttacttat tccgaccggg ccgccaaagc gagcggcggg gcaagctcgg
     1861 ccccgaccac gccgtagccg tagatcggat cgagtcttcg gccgccgccc ggatgctgag
     1921 cctgctgctc gagggcggcg agcgccgcat cggcggcagc cctgtccggg gaatgcagcg
     1981 cctgcgccag gaggcctgcg acgatcggcg cggcgaacga cgtcccgcgt accagcgcat
     2041 atccgcgcgg tgtccgcgcc gccgtcatgt ccgccccggg tgctgcgaat ctcacctgcg
     2101 ggccccgctc ggcctcggct aacacccgtt gccgcgcatc aacggccgtg acccccacca
     2161 ctcccggcca cgccgccgga tagagcggcg gcgcagcggg accgtcattg ccgaccgcgg
     2221 cgacgatgag atagccgcgt gcgaccgcgc ccgccacgac actctccagc agccgattgg
     2281 gtggcccgac gaggctcaca ttgatcaccg ccacgcgttc gcgaatcaac caggcgaaag
     2341 cctcggcgac ctcgtcgacg gcgccgccgg ccgcgcgccc gcagaagacg tcggcggcgt
     2401 agagctccgc acccggcgcc gcgccgcgga agctgccggc gcgtcctgca agcaaggagc
     2461 cgacggcggt gccgtggacg gccggcagcg cagttcccgc gcagccatgc gaatggatcg
     2521 tcaccgcgct gagcgccgcg tggcgcccat cgacgccgtc gtcgatcagt ccgacccgga
     2581 cggcggcggg gtgcggcgcg gcgcccgccg aaatgaccgg gagcgacggc acgggatccg
     2641 cggcgtccgc cggcgcgccg ctcggaagat agacgtgatt gaagtcgtag ctgccggcgg
     2701 aatcgaggcc gcgcaggcgc gcgagagagc gcgccgtgtc atcgggcgct
//
LOCUS       DATLKL010000699         1807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_155375, whole genome shotgun sequence.
ACCESSION   DATLKL010000699 DATLKL010000000
VERSION     DATLKL010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155375"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1279
                     /locus_tag="VM713_09445"
     CDS             161..1279
                     /locus_tag="VM713_09445"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HVH84527.1"
                     /translation="MSATAPATHAAITGWGKCLPPAVLSNHDLSTILDTSDEWIVSRT
                     GIRERRISHVGLGDLAYVAAARALACAGLEGSRIELIAFGSCSYDDQVPNMASGIQAR
                     IGAQGAAAYDINTACTSFLYGLSTATALIRTGVVRNALVIGAELISPFMDWSDRDVAV
                     LFGDGAAAVVLEASARQEGLLAEKLGCYGEAREILRVHGMGGRYANRGVLYGITRWQF
                     EGQEIFKRAVQGMSGASEEALARIGAKSADVDLVVPHQANLRIIEAVAKRTHVPMERV
                     YVTVQRYGNMSAATVPVALCEALEEGRVRAGALLLMPGFGGGLSFCAHAVRWGERTTP
                     LGASAEGLGPNERPALELVRECLARKIRPAPATTEPFA"
     gene            complement(1295..1414)
                     /locus_tag="VM713_09450"
     CDS             complement(1295..1414)
                     /locus_tag="VM713_09450"
                     /inference="COORDINATES: protein motif:HMM:NF028380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HVH84528.1"
                     /translation="MGKGDPRTRRGKIYQGSFGKSRPKDPAKRKKKAARAAKR"
     gene            complement(1522..>1807)
                     /locus_tag="VM713_09455"
     CDS             complement(1522..>1807)
                     /locus_tag="VM713_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HVH84529.1"
                     /translation="TKIYVGNLPFSATDAEVRELFAQHGTVESVSLITDRDTGRPRGF
                     GFVEMSRADASRAIQNLNGKELGGRPLRVNEAQERSGGGGNRGGGAPKRW"
BASE COUNT          268 a          636 c          626 g          277 t
ORIGIN      
        1 gagcaggcag tagagcccga tcagacgcgc gagaaacccg gtcttgtccg acatggtgct
       61 gactccgccc gacccgcggc gattgtagtg cgcgcatgat gcggcgcggc tgcgcatttc
      121 tacgtagccc gcagcgcccc tgctatcctt ggcgccgcca atgagcgcca cagcgccagc
      181 gacccacgcg gcgatcaccg gttggggaaa atgcctgccc ccggcggtgc tcagcaacca
      241 cgacctctcg accatcctcg acaccagcga cgagtggatc gtcagccgca cgggaatccg
      301 cgagcggcgc atctcgcacg tgggactcgg cgacctcgcc tacgtcgccg ccgcccgcgc
      361 cctcgcctgc gccgggctcg agggctcgcg catcgagctc atcgcgttcg ggagctgcag
      421 ctacgacgac caggtcccga acatggcctc cggcatccag gcgcgcatcg gcgcgcaggg
      481 ggcggcggcc tacgacatca acaccgcctg caccagcttt ctctacggcc tgtcgaccgc
      541 gaccgcgctc atccgcaccg gcgtggtacg caacgcgctc gtcatcggcg cggagctcat
      601 ctcacccttc atggactgga gcgaccggga cgtcgccgtg ctgttcggcg acggggccgc
      661 cgcggtcgtg ctcgaggcga gcgcacgcca ggagggcctg cttgccgaga agctcggctg
      721 ctacggggag gcgcgcgaga tcctgcgcgt tcacggcatg ggcggccgct acgcgaaccg
      781 cggcgtgctc tacggcatca ctcgctggca gttcgagggc caggaaatct tcaagcgcgc
      841 ggtgcagggc atgtcgggag cgagcgagga ggcgctggca cgcatcggag caaagtccgc
      901 ggacgtcgac ctcgtggtgc cgcatcaggc gaacctgcgc atcatcgagg cggtcgccaa
      961 gcgcacccac gtgccgatgg aacgcgtgta cgtgacggtg cagcgttacg ggaacatgtc
     1021 ggccgccacg gtgcccgtgg cgctgtgcga ggcgctcgag gagggccgcg tacgcgccgg
     1081 tgcgctgctc ctgatgccgg gattcggcgg cggcctctcg ttctgcgccc acgcggtgcg
     1141 ctggggcgag cgcacgacgc cgctcggtgc gagcgcggag ggactcgggc cgaacgagcg
     1201 cccggcgctc gagctcgtgc gggagtgtct cgcgcgtaag atccgcccgg cgcccgcgac
     1261 caccgagcct tttgcctgaa gggctgcggc gcgctcagcg cttggcggcc cgtgcggctt
     1321 tcttctttct cttcgccgga tccttggggc gcgacttccc gaacgacccc tggtagatct
     1381 tgccgcggcg cgtacgcgga tcgcccttac ccatgagaaa ccactcccct cgactcgatc
     1441 gatgatcggc tcatgcaacg aaaagggccg gccgccgatg ccgcgaggca ctgacggccg
     1501 gcccagaggc tcaactgctg cttaccagcg cttcggtgcg ccgccgccgc ggttgccgcc
     1561 gccgccgctg cgctcctggg cttcgttgac ccgcagtggg cgtccgccca gctctttgcc
     1621 attgaggttc tggatggcgc gcgaggcatc ggcgcgcgac atctcgacga acccgaagcc
     1681 gcgcggccgg ccggtgtcgc ggtcggtgat gaggcttacg gactcgacgg tgccgtgctg
     1741 agcgaacagc tcgcgaacct cggcgtcagt ggcggagaaa ggcaggttgc ccacgtagat
     1801 cttcgtc
//
LOCUS       DATLKL010000700         1759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_222321, whole genome shotgun sequence.
ACCESSION   DATLKL010000700 DATLKL010000000
VERSION     DATLKL010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222321"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1759)
                     /locus_tag="VM713_09460"
     CDS             complement(<1..>1759)
                     /locus_tag="VM713_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH84530.1"
                     /translation="SSLPSHARVVVVGGGIIGTSVAYHLATMGWKDVVLLERDRLTSG
                     TTWHAAGLMVTFGSTSETSTEMRKYSRDLYRRLEAETGQSTGLAQIGFIEVAADRDRL
                     EEYRRVAAFNRYCGVDVLEISPREVLELFPLARVDDIEAGFYVKEDGRVNPVDVTMAL
                     AKGARLKGVRIIEGVPAMGVAQARGRVTAVRTALGDIRTEIVVNCAGMWARQLGEASG
                     VTIANQAAEHYYLITEPIRNLPPRMPVLEDPGAYGYYREKGGGLLVGLFEPVCAPWKV
                     EGIPSDFSFGELPPDWERMTPFLERAMARVPITQEVGMKRFFCGPESFTPDLRPIVGE
                     APELRGYFVAAGLNSIGVLSGGGLGRVLAHWIVTGYPDVDVTGFNIDRLHVTQANPEY
                     RRARTVESLGMVYKCHYPFMSLTTARGVRKSPFHERLAAAGAYFKEVSGWESPDWYGE
                     PGTTPDPGPLSWGRPSWFARWREEHRAARDNVILMDMSFMGKFLVQGRDAGRLLNRIS
                     ANQVDGPAGVITYTQWLNETGRLEADLTVTKLGDERYLVVVTDTMVRHAETWMRRHLA
                     ADAHASVTDVTSAYGQLNVQ"
BASE COUNT          286 a          607 c          592 g          274 t
ORIGIN      
        1 ctgcacgttg agctgcccgt aggcggaggt gacatcggtg acggaggcat gcgcatccgc
       61 ggcgaggtga cgccgcatcc aggtttcggc gtggcgcacc atggtgtcgg tcacgaccac
      121 cagatagcgc tcatccccga gcttcgtgac cgtgaggtcg gcctcgagac ggccggtctc
      181 gttgagccac tgcgtgtagg tgatgacccc ggcggggccg tcgacctgat tggcggagat
      241 gcgattgagc aagcgcccgg catcgcgccc ctgcaccagg aattttccca tgaaggacat
      301 gtccatgaga atcacgttgt cgcgcgcggc gcgatgctcc tcgcgccagc gcgcaaacca
      361 gctcgggcgc ccccagctga gaggacccgg gtccggcgtg gtcccgggct ctccgtacca
      421 gtcgggactc tcccagccgc tcacctcttt aaagtaggcg cccgcggccg cgagccgctc
      481 gtggaaaggt gacttcctca cgccgcgcgc cgtcgtgagc gacatgaacg ggtagtggca
      541 cttgtacacc atgccgagcg attcgaccgt gcgtgcgcgg cggtactcgg gattggcctg
      601 cgtcacgtgc agccggtcga tgttgaatcc agtgacatcg acatcgggat agcccgtcac
      661 gatccagtgc gccagtacgc gccccagccc gccgccgctc agcacaccga tggagttgag
      721 ccccgccgcc acgaagtagc ccctgagctc gggcgcttcg ccgacgatcg gccgcaggtc
      781 cggggtgaag ctctccggcc cgcagaagaa gcgcttcatg ccgacctcct gggtgatcgg
      841 cacacgcgcc atggcgcgct cgaggaacgg cgtcatgcgc tcccagtcgg gcggcagctc
      901 gccgaaggag aagtcggaag ggatcccctc caccttccac ggcgcacaca ccggctcgaa
      961 gaggcccacc aagagcccgc cgcccttctc gcggtagtag ccgtaggccc ccgggtcctc
     1021 gagcaccggc atgcgcggcg gcagattccg aatgggctcg gtgatcaggt agtagtgctc
     1081 ggcggcctgg ttggcgatgg tgacgccgct cgcttcaccg agctgccgcg cccacatgcc
     1141 ggcgcagttg acgacgatct cggtcctgat gtcgccgagc gcggtgcgca ccgcagtcac
     1201 gcgcccgcgc gcctgcgcca ctcccatggc cggcacgccc tcgatgatcc tgacaccctt
     1261 gaggcgtgcg cctttggcga gcgccatggt gacgtcgacg ggattgacgc gcccgtcctc
     1321 cttcacatag aagccggctt cgatgtcgtc gacgcgcgcg agcggaaaga gctcgagcac
     1381 ctcgcgcggg gagatctcca gaacgtcgac gccgcaatag cggttgaacg ccgcgacgcg
     1441 ccggtactct tccagccggt cccggtcggc ggccacctca atgaagccga tctgcgcgag
     1501 ccccgtcgac tggccggtct cggcctcgag tcgccggtag agatcgcgcg agtacttgcg
     1561 catctcggtc gaggtctcgg aagtcgagcc gaaggtcacc atgagacccg cggcgtgcca
     1621 ggtggtgccc gaggtgaggc ggtcgcgctc gaggagcacg acgtccttcc agcccatcgt
     1681 cgccaggtgg taggccaccg aggtgccgat gatcccgccg ccgacgacca cgacgcgggc
     1741 gtgggagggg agactcgac
//
LOCUS       DATLKL010000701         2325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_166363, whole genome shotgun sequence.
ACCESSION   DATLKL010000701 DATLKL010000000
VERSION     DATLKL010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166363"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VM713_09465"
     CDS             complement(<1..343)
                     /locus_tag="VM713_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH84531.1"
                     /translation="MSAVGEGASRVLVVEDDPYLGAGVMENLRAEGYEVTAAGDGEQA
                     LEWLLTNRCALVVLDVMLPGVDGLAVCRTMRGRGDSTPVLFLTARGDPADRVRGLEAG
                     GDDYLSKPFHLQ"
     gene            complement(340..1422)
                     /locus_tag="VM713_09470"
     CDS             complement(340..1422)
                     /locus_tag="VM713_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVH84532.1"
                     /translation="MKATRVFQLAALVLLVVSAVQVSYWLFDQRDYTIEKVNAARAAY
                     AAQAAAAQALLDAGVSAARIHELLPQIVIDGGHASVARELEETLATERHRRYNRYAWE
                     GTFFLLALGACMFVIARALRAEARVLEDQENFLALVSHQFKTPLASLQLSLETMALRT
                     LSPEQSRTLIARMLADLARMEAMVTQILESARLERGRVDFKSEPVDLHAAVARVVVAL
                     EERARSQRVAISSDVAPGLYALADPLALDVIVRNVLENALAAVAPVGGGSIAVSARAG
                     NGEVELAVADSGVGFTPGDGARLFEKFSRLHPGGGSRHYGTGLGLYIVKRLMQLAAGR
                     VTAESAGLGQGARFVLTWPAAPAGES"
     gene            complement(1433..>2325)
                     /locus_tag="VM713_09475"
     CDS             complement(1433..>2325)
                     /locus_tag="VM713_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH84533.1"
                     /translation="DLIVKFAGCYHGHADHLLVAAGSGLATLGKPSSAGVPQAFTGCT
                     RVLPLDDEAALTALFEREGARIAAVIIEPVPANHGLLPQRREFLEALRRTMQAHGALL
                     IFDEVISGFRLARGGAAELLGIRPDLATFGKVIGGGMPVGAFGGEQRIMARLAPDGDT
                     YQAGTLSGNPVAMAAGLATLEVLERESGWQRLEARGAELERLLAPVLSRAPYPVTLVR
                     VGSLFWMSLHEHGAPRTAAPLSERKAGRFAALFHAMLARGVYLPPSAYEACFLSLAHS
                     SADLEEFAAALAASLAGHTT"
BASE COUNT          370 a          835 c          822 g          298 t
ORIGIN      
        1 cctgcagatg aaagggcttg gagaggtaat cgtcgccgcc ggcttcgagt ccccgcaccc
       61 ggtccgccgg atcgccgcgc gccgtcagga acagcaccgg cgtactgtcg ccccgaccgc
      121 gcatcgtgcg gcatacggcg aggccgtcga ctccgggcag catgacatcg agcaccacca
      181 gcgcgcagcg gttcgtgagc agccactcga gcgcctgctc gccgtcgccc gccgcggtca
      241 cctcgtagcc ctccgcgcgc aggttttcca tgacgccggc gccgaggtac ggatcgtcct
      301 ccaccaccag cacgcggctc gcgccctcgc caacggcgct catgactccc ccgccggggc
      361 cgccggccag gtgagcacga agcgcgcacc ctgcccgagt ccggcgctct cggcggtgac
      421 cctgccagcc gcgagctgca tcagccgttt gacgatgtag agcccgagcc cggtgccgta
      481 atggcggctg ccgccgcccg ggtggagccg cgagaacttc tcgaacaggc gcgcgccgtc
      541 tccgggcgtg aagccgacgc cgctgtccgc cacggcgagc tccacctcgc cgttgccggc
      601 gcgggcgctg accgcgatgc tgccaccccc gaccggagcc accgcggcga gcgcgttctc
      661 gaggacgttg cgcacgatga cgtcgagggc gagcggatcg gcgagcgcat aaagtcccgg
      721 cgcgacgtcg ctcgagattg cgacgcgctg cgagcgggcg cgctcctcga gcgccacgac
      781 cacgcgcgcg accgcagcgt gcagatctac cggctcggac ttgaaatcga cacgcccccg
      841 ctcgagacgg gcgctctcga ggatctgggt caccatcgct tccatgcgcg ccagatccgc
      901 gagcatcctg gcgatcaagg tgcgcgactg ctcgggcgag agcgtgcgca gcgccatggt
      961 ctcgagcgag agctgcaggc tggcgagcgg ggtcttgaac tggtgggaca cgagcgcgag
     1021 gaagttctcc tgatcttcca gcacccgcgc ctcggcgcgc agcgcgcgcg cgatcacgaa
     1081 catgcacgcg ccaagggcca gcaggaagaa ggtaccttcc caggcgtagc ggttgtaacg
     1141 ccgatgccgc tcggtggcga gcgtctcttc gagctcgcgc gccaccgagg catggccacc
     1201 gtcgatgacg atctgtggca gcagctcgtg tatgcgcgcg gcgctcaccc cggcatcgag
     1261 cagcgcctgc gcggcggcgg cctgcgccgc ataggccgcg cgcgcggcat ttaccttctc
     1321 gatggtgtag tcgcgctgat cgaatagcca gtaactcacc tgcaccgccg acacgaccag
     1381 cagcacgagc gcggcgagct gaaagacgcg ggtggccttc atggtggacc ggtcacgtcg
     1441 tgtgcccggc gagggaggcc gcaagcgccg cggcgaactc ctcgaggtcg gcgctcgagt
     1501 gtgcgaggga gaggaagcag gcctcgtagg ccgaaggcgg cagatagacg ccgcgcgcga
     1561 gcatggcgtg aaagagcgca gcgaagcgcc cggccttgcg ctcgctgagc ggcgccgcag
     1621 tgcgcggcgc gccgtgctcg tgcaacgaca tccagaagag cgagcccacg cgcaccagcg
     1681 tgacggggta aggagcgcgg gacagcacgg gtgccagcag ccgctcgagc tcggcgccgc
     1741 gcgcctcgag ccgctgccag ccggactcgc gctcgagcac ctccagcgtc gcgagccccg
     1801 ccgccatggc gaccgggttc cccgagagcg tgcccgcctg gtacgtatcc ccgtccgggg
     1861 cgaggcgcgc catgatgcgc tgctcgccgc cgaaggcgcc gaccggcatg ccgccgccga
     1921 tcaccttgcc gaaggtggcg agatcggggc ggatgccgag gagctccgcg gcgccgcccc
     1981 gcgccaggcg aaagcccgag atcacctcgt cgaagatgag gagcgcgccg tgcgcctgca
     2041 tcgtgcgccg cagggcctcg agaaactcgc gccgctgcgg cagcagcccg tgattggccg
     2101 gcaccggctc gatgatgacg gcggcgatgc gagcgccctc gcgctcgaac agtgccgtga
     2161 gggcggcttc atcatcgagg ggcagcacgc gcgtgcagcc agtgaaggcc tgcggcactc
     2221 cggccgagga gggcttgccg agtgtcgcca gccccgaacc tgccgcgacc agcagatgat
     2281 ccgcatgtcc gtggtagcag ccggcgaatt tcacgatgag gtcgc
//
LOCUS       DATLKL010000702         2162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_42332, whole genome shotgun sequence.
ACCESSION   DATLKL010000702 DATLKL010000000
VERSION     DATLKL010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42332"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VM713_09480"
     CDS             complement(<1..435)
                     /locus_tag="VM713_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84534.1"
                     /translation="MSGSRREFPTTSCAVLLAPGAAAAGTADPTPTPTSPAEPPPGSP
                     PAFGTSPPVGPEVSAATFADAEKLVMVELTAAECAQAAENWRSSMATIHERRSGPRKV
                     ALPPTLAPYSTWNPLLPGQGALPQAERFLRSERDAGPLPAADA"
     gene            497..>2162
                     /locus_tag="VM713_09485"
     CDS             497..>2162
                     /locus_tag="VM713_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HVH84535.1"
                     /translation="MPRNKQTLALSPALAVGALGVVFGDIGTSPLYTFETVLDLTGSH
                     DAPTILGVVSLLVWTLIIVTGVKYAGLAMSIDNDGEGGILALMALLGVKRYKRPLIVA
                     VGLFGAALIYGDGAITPAISVLSALEGLTLVEPALQPYVLPLTVGVLLALFLVQPLGT
                     ARIGRAFGPVMALWFVTIAALGVLGVARHPAVLWALNPWYACDYLRHGGHTAFAVLGG
                     VFLCVTGAEALYADMGHFGARPIRLAWSALVFPSLVLNYAGQSAIILAGAPTEGNIFY
                     RLCPGQWLVPMVLLSTLATIIASQSIITGAFSMTRQAIQLGWMPRLKVVQTSGEGYGQ
                     IYVGTVNWLLMLATVALALGFRKSDNLAGAYGIAVSATMLLTTALLFTAMRDIWKWGL
                     AASLGVAAVFFAIDGSFFASNMLKLLEGGYVPLLLAAAVYGVMYIWHRGLVAIRERVH
                     EHAVPVADFFAGIARDGVARVPGTAVFLTRVKNATPPVMLWYVRHSHALHEKVIAVTL
                     ETASVPWVRAEDRIQLGQPVPDFWVASASYGFMQRPDMPDLMRQLAA"
BASE COUNT          288 a          747 c          746 g          381 t
ORIGIN      
        1 ggcgtcagcg gcgggcagcg gccccgcatc gcgctcgctg cggaggaagc gctcggcctg
       61 tgggagcgcc ccctgaccgg gaagcaacgg gttccaggtc gagtagggcg cgagggtcgg
      121 tggcagtgcc accttgcgag ggccgctgcg tcgctcgtgg atggtcgcca tgctgctgcg
      181 ccagttctcg gcggcctggg cgcactccgc cgccgtcagc tccaccatca cgagcttctc
      241 cgcgtcggcg aaggtcgcgg cgctcacctc cggacccacc gggggtgagg tgccgaaggc
      301 aggtggtgag ccgggcggcg gctcggcggg cgacgtggga gtcggcgtcg ggtccgccgt
      361 cccggcagcg gccgccccgg gtgcgagcag cacggcacag gaagtcgtgg gaaactcgcg
      421 gcgcgatcct gacacggtgt cccctctctc tgttgtcttc gagcggggag tctaagcccg
      481 aacggtccgc cctcgtatgc cacggaacaa gcagacgctg gccctgtcac ccgccctcgc
      541 cgtgggcgca ctcggcgtcg tgttcggcga tatcggcacg agtcccctct acaccttcga
      601 gaccgtgctc gatctcaccg gcagccacga cgcgcccacc atcctcggcg tcgtgtcgct
      661 gctggtgtgg accctgatca tcgtcacggg cgtcaagtac gccggattgg cgatgagcat
      721 cgataacgac ggcgagggcg gcatcctcgc cctgatggcg ttgctcggcg tcaagcgcta
      781 caagcgcccg ctcatcgtgg cggtcggcct gttcggcgcg gcactcatct acggcgacgg
      841 ggcgatcacc ccggcgatct cggtgctgtc cgcgctcgag ggcctgacgc tcgtcgagcc
      901 cgcgctgcaa ccctacgtac tgccgctcac cgtcggcgtg ctcctcgccc tcttcctggt
      961 gcagccgctc ggcaccgcgc gcatcgggcg tgccttcggc ccggtgatgg ccctgtggtt
     1021 cgtgaccatt gcagcgctgg gggtcctcgg cgtggcgcga catccggccg tgctatgggc
     1081 gctcaacccc tggtacgcct gcgactatct ccgccacggc gggcacaccg cttttgcggt
     1141 cctcggcggg gtgtttctgt gcgtcacggg agcggaagcg ctgtacgccg acatgggcca
     1201 tttcggtgcc cggcccatca ggctcgcctg gtcggcgctg gtatttccga gtctggtgct
     1261 caactacgcc gggcagagcg cgatcatcct cgcgggggcg ccgaccgaag gcaacatctt
     1321 ctaccgcctg tgtcccggcc aatggctcgt gccgatggtg ctgctgtcga ccctggcaac
     1381 gatcatcgcg agccagtcga tcatcaccgg tgctttttcc atgacgcgtc aggcgatcca
     1441 gctcggctgg atgccgcggc tgaaggtggt gcagacctcc ggcgagggct acggacagat
     1501 ctacgtcggc acggtcaatt ggctcctgat gctcgcgacc gtggctctcg cgctcgggtt
     1561 ccgcaagtcc gataacctgg ccggcgccta cggcatcgcc gtatcggcca cgatgctgct
     1621 cacgacggcg ctgctcttca ctgcgatgcg cgacatctgg aagtggggcc tggcggcgag
     1681 cctcggcgtc gccgcggtgt tcttcgcgat cgacggcagc ttcttcgcct ccaacatgct
     1741 gaagctcctc gaggggggct acgtgccgct gctgctggcc gcggcggtct acggagtgat
     1801 gtacatctgg caccgcggcc tcgtcgcgat ccgcgagcgc gtgcacgagc acgcggtgcc
     1861 ggtcgcagac ttcttcgccg gcatcgcgcg cgacggggtc gcccgggtgc cggggacggc
     1921 cgtgttcctc acgcgcgtga agaatgccac gccgcccgtc atgctctggt acgtccgcca
     1981 cagtcacgcg ctgcacgaga aagtgattgc ggtgacgctc gagacggcgt cggtcccctg
     2041 ggtaagagcg gaagatcgca tacagctcgg gcagccggta cctgacttct gggtggcgag
     2101 cgcctcctac ggcttcatgc agcggccgga catgccggac ctcatgcgcc agctcgccgc
     2161 gc
//
LOCUS       DATLKL010000703         2405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_132653, whole genome shotgun sequence.
ACCESSION   DATLKL010000703 DATLKL010000000
VERSION     DATLKL010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132653"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2360)
                     /locus_tag="VM713_09490"
     CDS             complement(<1..2360)
                     /locus_tag="VM713_09490"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84536.1"
                     /translation="MLDIRREGADGRRASIAEVLEMTVTEAGAFFADAAEVAARLAPL
                     ADVGLEYLKLGQPVPTLSGGEAQRLKLAGHLAAAGSVLSSTTHKGKLFLFDEPTTGLH
                     FEDVAKLLKAFRKLLAAGHSLLVIEHNLDVIGAAEWIIDLGPEGGERGGEIVCTGTPA
                     EVSAVDRSHTGRALAAYRAALAAHVPAVPEARLPEAAVAEPRAAYAGNGRGRNAVVIH
                     KAREHNLKGIDVEIPRDCFTVITGVSGSGKSTLAFDILFNEGQRRYLESLNAYARQFV
                     QPAARPDVDAIFGIPPTVAIEQRTSRGGRKSTVATLTEIYHFMRLLYVKLGTQYCPDC
                     NVAIEPQSLGVIAARLLRDYRGKSITLLAPLVVARKGYYTDLAKWAAKKGFRTLRVDG
                     ELTPTAPWPRLSRFREHTIELPVAELAVGAKTDAALHEALARALDFGKGLLHVLTPKS
                     AAVTVYSTKRACPSCGTSFAELDPRLFSFNSRHGWCASCYGTGVEMPGFDAEQSGEEL
                     WWNEWYDREPPACSVCAGQRLNPIALNVRFRGRSIAALSSQSVAASAEFFAHLRLKPR
                     EREIARDLLAEIRARLRFLSRVGLAYLELDRSAPTLSGGEAQRIRLAAQLGSNLQGVC
                     YVLDEPTIGLHPRDNAILLDSLAGLAANRNTLVVVEHDEETIRRADHVLDLGPQAGVR
                     GGELIAAGSVRDLMANPRSTTGRFLRAPLLHPARPHRAVTPASALLTLERVTLHNLRG
                     GDVRIPLGRLVVITGVSGSGKSTVARDVLYASLKRLLAGAGGPLRR"
BASE COUNT          352 a          825 c          888 g          340 t
ORIGIN      
        1 ttgcggcgca gcgggccgcc ggcgccagcg agcagccgct tcaggctcgc gtacagcaca
       61 tcgcgcgcca cggtcgactt gcccgagccc gagacgcccg tgatcaccac cagccgcccg
      121 aggggaatgc gcacgtcgcc accgcgcagg ttgtgcagcg tcacgcgctc gagcgtgagg
      181 agcgcgctgg cgggggtcac ggcgcggtgc ggccgcgctg gatggaggag cggcgcgcgc
      241 aggaagcgcc cggtggtcga gcggggattg gccatgaggt cgcgtacgct gccggcggcg
      301 atgagctccc cgccgcgcac gcccgcctgc ggacccaagt ccaggacgtg gtcggcgcgg
      361 cggatggtct cctcgtcgtg ctcgacgacg accagcgtat tgcggttggc ggcgagcccg
      421 gcgagggagt cgaggagaat ggcgttgtcg cgcgggtgca gcccgatggt cggctcatcg
      481 agcacgtagc acacgccctg gagattcgag ccgagctggg cggcgagccg gatgcgctgc
      541 gcttcgccgc ccgagagcgt cggcgcggag cggtcgagct cgaggtaggc gaggccgacg
      601 cggctgagga accggagccg cgcgcggatc tcggccagga ggtcacgggc gatctcccgc
      661 tcgcgcggct tcaggcgcag gtgcgcgaag aactccgcgc tggccgccac cgactgggac
      721 gacagcgcgg cgatcgagcg cccgcggaag cgcacattca gggcgatcgg gttcaggcgc
      781 tggcccgcgc acacgctgca ggcgggcggc tcgcggtcgt accactcgtt ccaccacagc
      841 tcctcgcccg actgctcggc gtcgaagccc ggcatctcga ctccggtgcc gtagcagctc
      901 gcgcaccagc cgtggcgcga gttgaaggag aacaggcgcg ggtcgagctc ggcgaagctc
      961 gtgccgcagg aggggcaggc gcgcttggtg gagtacaccg tgaccgccgc ggacttcggc
     1021 gtcagcacgt ggaggagccc cttgccgaaa tcgagcgccc gcgcgagcgc ctcgtgcagc
     1081 gccgcgtcgg tcttggcgcc caccgccagc tccgccaccg gcagctcgat cgtgtgctcg
     1141 cggaagcgcg acaggcgcgg ccacggcgcg gtgggtgtga gctcgccatc cacacgcagc
     1201 gtcctgaagc ccttcttcgc cgcccacttc gccaggtccg tgtagtaacc cttgcgtgcc
     1261 acgacgagcg gcgcgagcag cgtgatcgat ttgccgcggt agtcgcgcaa cagccgcgcg
     1321 gcgatcacgc ctaagctctg cggctcgatc gcgacgttgc agtccgggca gtactgcgtg
     1381 ccgagcttca cgtagaggag gcgcatgaag tggtagatct cggtgagcgt cgccacggtg
     1441 ctcttccggc cgccgcggct ggtgcgctgc tcgatggcca cggtcggcgg aatgccgaag
     1501 atcgcatcca cgtcgggtcg tgcggccggc tgcacgaact gccgtgcata ggcgttcagc
     1561 gactcgagat agcggcgctg cccttcgttg aagagaatgt cgaaggcgag cgtcgatttg
     1621 ccggagcccg acacgcccgt gatcaccgtg aagcaatcgc gtggaatctc gacgtcgatg
     1681 cccttcaggt tgtgctcgcg ggccttgtgg atgacgaccg cattgcggcc acggccgttg
     1741 ccggcgtagg ccgcacgcgg ctcggcgacg gcggcttcgg ggaggcgcgc ctcgggcacg
     1801 gccggcacgt gcgcggcgag cgccgcccgg taagcggcga gcgcccgccc ggtgtgcgag
     1861 cggtccacag cgctcacctc ggccggcgtt cccgtgcaga cgatctcgcc gccgcgctcg
     1921 ccgccctcgg ggcccaagtc gatgatccac tcggcggcac cgatcacgtc gaggttgtgc
     1981 tcgatcacga gcagcgagtg ccccgcggcg aggagcttcc tgaaagcctt gagaagcttc
     2041 gccacatcct cgaagtgcag tcccgtggtc ggctcgtcga agagaaacag tttgcccttg
     2101 tgcgtggtgc tcgagagtac cgagccggcc gcggcgagat gaccggcgag cttcaggcgc
     2161 tgcgcctcgc cgcccgagag cgtcggcacc ggctgaccga gcttgaggta ctcgagcccg
     2221 acgtccgcga gcggcgccag ccgcgcggcc acctcggcgg cgtcggcgaa gaacgcgccc
     2281 gcctcggtca cggtcatctc gagcacttca gcgatgctgg cgcggcggcc gtcggcgcct
     2341 tcgcggcgga tgtcgagcac ctcgtcgcga taacgccggc cgtcgcagtc cgggcagcgc
     2401 agata
//
LOCUS       DATLKL010000704         1836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_200080, whole genome shotgun sequence.
ACCESSION   DATLKL010000704 DATLKL010000000
VERSION     DATLKL010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200080"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VM713_09495"
     CDS             complement(<1..189)
                     /locus_tag="VM713_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84537.1"
                     /translation="MIVDRLARQALRVCCRESAHVLDQRLESTGIYLHGPPRIRAHPL
                     TRNFFDQLSALVAIRQSEH"
     gene            211..1065
                     /locus_tag="VM713_09500"
     CDS             211..1065
                     /locus_tag="VM713_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVH84538.1"
                     /translation="MPLVMQAKYELLNHGGVLRYEHDTAQLSDVGGLVNFKAWLSKRC
                     SAFDGSAPELDPPKGVLLLGVQGCGKSLAARASAGIFHVPLLRLDCGALYDKYIGESE
                     RNLRESLASADVLAPCVLWIDEIEKGFAAGDSDGGATRRVLGAFLTWLAEKSSRVFVV
                     ATANDIAELPPELIRKGRFDEIFFVDLPSLSTRAEILRIHAARRGIALDAASLGTLAR
                     ECEGFSGAEIEQAIVAALYSAHAKHAAPDAQLLGAEIAATRPLAVVMAESVAALRDWA
                     KDRTVPAD"
     gene            complement(1062..1781)
                     /locus_tag="VM713_09505"
     CDS             complement(1062..1781)
                     /locus_tag="VM713_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVH84539.1"
                     /translation="MSFLNVYDDARRADAYATLEFPGTYWLAFRDLPAIITRYVTGSV
                     ALDFGCGAGRSTRFLKRLALDATGVDVSASMIEHARTADPAGSYRLIGDGDFSGLEAE
                     SLDLVFSAFAFDNIADAARRAELLGGLRRLLKRAGRVILLDSTAEIYTHEWVSFSTSA
                     FPENRVARSGDAVRIVMTDVVDQRPVVDYIWFHEDYLRLFDAAKLRLIAQHRPLGTEG
                     EPWRWKSELSVAPWVIYVLAS"
BASE COUNT          336 a          578 c          609 g          313 t
ORIGIN      
        1 gtgctcgctc tgccggatcg caacgagcgc cgacagctgg tcgaagaagt tgcgcgtcag
       61 tggatgcgcg cgaatccgcg gcgggccgtg aagatagatg cccgtgctct cgagccgctg
      121 gtcgagaaca tgagcggact ctcggcagca gacacgcagc gcctggcgcg caaggcgatc
      181 tacgatcacg gtacgatcga gctctcggac gtgccactgg tgatgcaggc gaagtacgaa
      241 ctgctaaacc atggtggcgt cctgcggtat gagcacgaca ccgcccagct ttccgatgtc
      301 ggcggcctcg tgaatttcaa agcgtggctt tcgaagcgct gctccgcctt cgacggctcg
      361 gcgccggagc tcgacccccc caaaggcgtg ctgttgctcg gagtacaggg ctgcggcaag
      421 agtctcgcgg cgcgggccag tgccggcatc ttccatgtgc cgctgctacg gctcgactgc
      481 ggagcgcttt acgacaagta catcggcgaa tccgagcgca acctgcgcga gtcgctggcg
      541 tccgcggacg tgctcgcgcc gtgcgtgctg tggatcgatg agatcgagaa gggcttcgct
      601 gccggggact ccgatggcgg ggcgacccga cgggtgttgg gtgcgttcct tacctggctg
      661 gcggagaaga gcagccgggt gtttgtggta gccaccgcca acgacatcgc tgagttgccg
      721 cccgagctga tccgcaaggg gcgctttgac gagatcttct tcgtcgatct cccgagcctg
      781 agcacgcgtg cggaaatcct tcgcatccac gcggccaggc gcggtatcgc gctggacgct
      841 gcgagcctcg gcacgctggc gcgggagtgt gagggttttt cgggcgcgga gatcgaacag
      901 gcgattgttg ccgcgctcta cagcgcgcac gccaagcacg ccgcgcccga tgcgcagctg
      961 ctgggtgccg agatcgccgc cacgcgaccg ctggcggtgg ttatggccga aagtgtggcc
     1021 gcccttcgcg actgggcgaa ggatcggacg gtgcccgccg attaggaagc caggacgtaa
     1081 attacccagg gagcaaccga gagctctgac ttccagcgcc agggctcacc ctccgttccg
     1141 agcggcctgt gctgagctat caggcgcaat ttggccgcat cgaacagccg caaatagtcc
     1201 tcgtgaaacc agatgtagtc cactaccgga cgttgatcga cgacgtccgt catcacgatg
     1261 cgtaccgcgt cgccactcct ggctacgcgg ttctctggaa aggcgctggt agagaaggat
     1321 acccactcgt gtgtatagat ctccgcggtc gagtcgagca ggatgactcg cccagcgcgc
     1381 ttgaggagcc tgcgcaggcc ccccaatagc tccgcccgcc gtgccgcgtc ggcgatgttg
     1441 tcgaacgcga atgccgagaa caccagatcg aggctctcgg cctccaggcc gctgaaatca
     1501 ccatccccga tcaggcgata cgatccggcg gggtccgccg tccgagcatg ctcgatcatg
     1561 ctcgccgaga catcgactcc agtggcatca agcgcgagtc gcttgagaaa acgtgtggag
     1621 cggcccgctc cgcaaccgaa atcgagggcg accgatccgg tcacgtaccg cgtgatgatg
     1681 gccggcagat cgcggaaggc gagccagtag gttcccggga actcgagcgt cgcgtatgcg
     1741 tcggcccgcc gcgcatcgtc atagacgttc aggaacgaca aacagggtct ccttcagtgc
     1801 gcccgactat aggacgtcac tcaaggcggg caagcc
//
LOCUS       DATLKL010000705         1539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_121318, whole genome shotgun sequence.
ACCESSION   DATLKL010000705 DATLKL010000000
VERSION     DATLKL010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121318"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VM713_09510"
     CDS             <1..1304
                     /locus_tag="VM713_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVH84540.1"
                     /translation="TESFYARLDGESEWRRLAKFEVFNAKNTFEPVAISRDEPNKAYA
                     FANSHGRKALWLIDLRDQDDPLLVFADPNVDVDSAVRGPDAHLVGVYYESTYPSMFYL
                     DQREQAVAAAVRKARGGQFTTVVERTQDENLYILKSESDLVPDLYSLFDVRGGRLIKI
                     GGPQAGLNPEDMSPLQAISYPARDGVQIPGYLTLPRAGAKDHLPLVVMPHGGPIARDG
                     WHYDFLRQFLASRGYAVLQMNFRGSDGYGSEWFFAAHQDWGGKTYEDVVDGAKWAIAS
                     GLADPRRVAIVGWSFGGYVALVGAQRDGELFRCAVSVAGISDLGLLIDEESHYINLGE
                     VARKQIGTNKEKLRRDSPRLHAADVQVPVLMIHGNLDAQVDLAQSQAMDTALSRAGKP
                     HRLVTIKDADHQMSAESARLTLLREIETFLAAHIPAPGLSQ"
BASE COUNT          284 a          492 c          490 g          273 t
ORIGIN      
        1 cgaccgagtc tttttatgca cgcctggacg gcgaatcgga gtggcggcgg ctcgcgaagt
       61 tcgaggtctt caacgccaag aacaccttcg agccggtcgc gatcagtcgc gatgagccca
      121 acaaggcata tgccttcgcg aactcccacg gccgcaaggc gctgtggctc atcgacctca
      181 gggaccagga tgatccgctg ctggtgttcg ccgaccccaa tgtcgatgtc gacagtgccg
      241 tgcgcgggcc cgacgcgcat ctcgtcggtg tctactacga gagcacgtac ccgagcatgt
      301 tctatctcga tcagcgcgaa caggcagtcg cggcggcagt gcgcaaggcc aggggcggcc
      361 agttcaccac ggtcgtcgag cgcacgcagg acgagaacct ctacatactg aagtcagaga
      421 gcgacctcgt gcccgatctc tacagcctgt ttgacgtgcg cggcggccga ctgatcaaga
      481 tcggcggccc gcaagcggga ctcaaccccg aggacatgtc tcctctgcag gcgatcagct
      541 atccggcgcg cgacggcgtg cagataccgg gctacctgac gttgccgcgc gcgggcgcga
      601 aggatcacct gccgctcgtc gtcatgccgc acggcggccc gatcgcgcgc gacggctggc
      661 attacgactt tctgcgccag ttcctcgcca gccgcggcta cgccgtcctg cagatgaact
      721 tccgcggctc ggacgggtac ggctcagagt ggttctttgc ggcacatcag gattggggcg
      781 gcaagaccta tgaagacgtc gtcgacggcg ccaagtgggc aattgcctct ggtctcgccg
      841 atccgcggcg cgtcgcgatc gtcggctgga gcttcggcgg ctacgtagcg ctggtcggtg
      901 cgcagcgcga cggcgagctg ttccgctgtg cggtaagtgt cgcgggcatc agcgatctcg
      961 gcctcctcat cgatgaggag tcccactaca tcaatctcgg ggaggtggcg cgcaagcaga
     1021 tcggtaccaa caaggagaag ctccgccggg attcgccacg gctgcatgcc gccgacgtac
     1081 aggttccggt gctcatgatt cacggcaacc tggacgcgca agtcgatctc gcgcaatcgc
     1141 aggccatgga tacggcgctc agccgtgccg gcaagcccca tcggctggtc acgatcaagg
     1201 atgcggatca tcagatgagc gctgagtccg cacgcctcac gctgctgcgt gagatcgaga
     1261 ccttcctggc cgctcatatt ccggctccag gcctcagcca gtagcgctct gctcgcgcgg
     1321 cacggagagc ggacggattt atggggtcta ttccccgcca ggatggggca aacccccagg
     1381 gacataatcg acataatcac ccatcaccac tcaggcacag tccccggttc tggggtaatt
     1441 tctgcacgtc attgcgcatg gcagcctaga attgaacccg ggctatctag gtgcgaaagg
     1501 ttgcgcagct cgtgcgctac gcgatcgagc gtcgggtga
//
LOCUS       DATLKL010000706         1977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_200118, whole genome shotgun sequence.
ACCESSION   DATLKL010000706 DATLKL010000000
VERSION     DATLKL010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200118"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VM713_09515"
     CDS             complement(<1..471)
                     /locus_tag="VM713_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84541.1"
                     /translation="MQLALEQRLKLPGIALLGALCGVVLAAGGLTAFYLCASLLACAF
                     VLADFRCGVVLLILLMPLSGSSTLFPHEMFGIVGLNPLNLLLVGTCCSWVLQAFAGGS
                     LRHLLPRPLLLLYVLPVVVAGAIGTRHIHEIAPTLLVVYQGLDFADAGSYLREMM"
     gene            complement(487..1374)
                     /locus_tag="VM713_09520"
     CDS             complement(487..1374)
                     /locus_tag="VM713_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84542.1"
                     /translation="MRAGSEAEPLEGTPVEPMRRDLRIGSILAAEGKLDGTRIGQVLR
                     LQQMRRGLRFGEAAVSLGLITREDLSRALARQYDFPYQRQNGATVSPDLVVFRDAQHP
                     RVERLRALRTQLLIRWSNLATKPRALALVSPGSGEGRSHVAANLAVLFAQLGLRTLLI
                     DADLRAPRQHRIFDIPDRGGLSAVLSSRAAGEVPLPAFGSLSVLPAGAPPPNPQELLL
                     RPALASYLELASAQYDVVLIDTPPARTCADALGVAFCAGSALVLARTDHTRLSEAAVL
                     ARGLAAGGTLVLGAALNTF"
     gene            complement(1381..>1977)
                     /locus_tag="VM713_09525"
     CDS             complement(1381..>1977)
                     /locus_tag="VM713_09525"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HVH84543.1"
                     /translation="LHGEEKLRELSTQYGANHPAYQRQVSQNLELRGKLQAEQRKVAA
                     SLTNPDAQPLPRLASAQSAQDPAQARMLEPVLQHNVESAEHAYDTALQRYFSSQIDSR
                     ASQTNVTVLSAAAVPFNPYRPNIPLNLALSLVTGLGLGVALVALLEKTDARVRSAEDL
                     MFALQVPLLAELGDEGRPAGLLPSPAPADGVRALPRPG"
BASE COUNT          335 a          665 c          700 g          277 t
ORIGIN      
        1 catcatctcc cgcagatagc tcccggcatc agcgaaatcg aggccctggt agacgacgag
       61 cagcgtgggc gcgatctcgt ggatgtggcg cgtgccgatg gcgccggcca cgacgaccgg
      121 cagcacgtaa agcaggagca gcggccgcgg cagtaagtgg cgcaggcttc cgccggcgaa
      181 ggcctgcagc acccacgagc agcaggtccc gaccagcagc aggttgagag gattcaagcc
      241 gacgatgccg aacatctcgt gcgggaacag cgtgctcgag cccgagagcg gcatgagcag
      301 aatcagcagc acgacgccac agcggaagtc cgccagcacg aaagcgcacg cgaggagcga
      361 ggcgcacaga tagaacgcgg tcagaccgcc ggccgcaagc accactccgc acagcgcgcc
      421 gagcagcgcg atccctggca gtttgaggcg ctgttccagg gccagttgca tcgttggcgg
      481 cccggctcag aacgtgttga gcgcggcacc gagaacgagc gtgccgccag cggccagacc
      541 ccgcgcgagc actgcagcct cggagaggcg ggtgtgatcg gtgcgcgcca gcaccagcgc
      601 actgccggca cagaaggcca cgccgagcgc gtcggcacag gtgcgcgccg gcggggtgtc
      661 gatcaggacc acgtcgtact gggcgctggc gagctcgagg tagctcgcca gcgccggtcg
      721 cagcagcagc tcctgcgggt tcggtggcgg cgcacccgcc ggcagcactg ataaggatcc
      781 aaaggctggc aacggcactt cgcccgcggc ccggctcgac agcactgccg agaggccgcc
      841 gcgatccgga atgtcgaaga tccggtgctg gcgcggcgcc cgcaggtcgg cgtcgatgag
      901 gagcgtgcgc agcccgagct gcgcgaacag caccgcgagg ttggcagcga cgtggctgcg
      961 gccttcgccc gagccggggc tcacgagcgc cagtgcccgc ggcttggtcg cgagattcga
     1021 ccagcggatg agcaattgcg tccgcagcgc tctcaggcgc tcgacacgcg gatgctgcgc
     1081 gtcacggaac acgaccagat ccggactgac ggtcgccccg ttctgtcgct ggtagggaaa
     1141 gtcgtactgc ctggcgagcg ccctgctcag gtcctcgcga gtgatcagtc cgaggctgac
     1201 cgccgcctcc ccgaaccgca gaccgcgccg catctgctgc agtctcagca cctggccgat
     1261 gcgtgtgccg tcgagcttgc cctccgcggc gagaatgctg ccgatgcgca gatcccgccg
     1321 catcggttca actggcgtgc cttcgagtgg ctcggcctcg ctaccggctc tcatcacacc
     1381 tcagcccggc cgcggcaacg cgcggacgcc gtccgctggc gccggcgatg gcagcagacc
     1441 cgcgggcctg ccctcatcgc cgagctccgc aagcaacggc acctgcaagg cgaacatcag
     1501 atcctcggcg ctgcgcacgc gcgcgtccgt tttctcgagc aaggcgacca gcgccacgcc
     1561 taaccccagg ccggtgacga gagagagtgc caggttcagc gggatgttgg gacggtaggg
     1621 gttgaacggc acagccgccg cgctcagcac ggtgacgttc gtctggctcg cgcgactgtc
     1681 gatctggctc gagaaatagc gctgcagggc ggtgtcgtag gcatgttcgg ccgattcgac
     1741 gttgtgctgc aggaccggct cgagcatgcg cgcctgggcg ggatcctgcg ccgactgcgc
     1801 tgacgccagg cgcgggagtg gctgggcgtc gggattcgtg aggctcgcag cgactttacg
     1861 ctgctcggcc tgcagtttgc cgcgcagctc gaggttctgc gacacctggc gctggtaggc
     1921 gggatggttt gcgccgtact gcgtggagag ctcgcggagc ttctcctcac cgtgcag
//
LOCUS       DATLKL010000707         3773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_189146, whole genome shotgun sequence.
ACCESSION   DATLKL010000707 DATLKL010000000
VERSION     DATLKL010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189146"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1149)
                     /gene="hppD"
                     /locus_tag="VM713_09530"
     CDS             complement(88..1149)
                     /gene="hppD"
                     /locus_tag="VM713_09530"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017725398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HVH84544.1"
                     /translation="MPIMTASTDNPMGTDGFEFVEYTAPDPQLLRTLFERLGFPVVAR
                     HRSKNVTLHRQGEINFIINAEPASFAQGFARLHGPSACAMAFRVRDAAGAFRRALELG
                     AKPGPQSVGPMELNIPCIEGIGGSLIYLVDLYGERSIYDVDFRPVATPPPAEAAGLTV
                     IDHLTHNVGRGRMDVWAGFYEKLFNFREIRYFDIEGKLTGLLSRAMTSPCGKIRIPIN
                     ESQDDKSQIEEYLREYHGEGIQHIALATADIYHTVDVLRAHGVAFQDTPDSYYDGVAA
                     RVPGHRESLGELRQRRILIDGNPAKGEGVLLQIFTQNVIGPIFFEIIQRKGNEGFGEG
                     NFRALFESIEQDQIRRGVI"
     gene            1189..>3773
                     /locus_tag="VM713_09535"
     CDS             1189..>3773
                     /locus_tag="VM713_09535"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HVH84545.1"
                     /translation="MHARVASDDSRLLRSIPAARVALIERIARAASGRGSRNELHQRF
                     LRGYFHGVAEEDLAAHPPGQLAKAALAHLALGMRRAPGRALVRVFNPVAGADGFESPH
                     TLVLTVTDDMPFLVDSLGMALSGAGLAVHLIVHPVLQVRRDGRGRLTEIGANGSQAVH
                     PESWQLYEIDRATDPQQLEKLRRELESTLADVRAVVSDFMPMRERVREIIARLETDPP
                     PLPAAEVNEARHLLDWMEGRHFVFLGYRHYRLERGRSEDRLIPDVLSSLGVLARGRGR
                     AGRSAATVLRGDVRARARDPELLIVTKANSIATVHRAEYLDYVGIKTFDSRGLVDGEH
                     RFLGLWTSTAYHGSPREIPVLRRKVERVIEHFGLDPASHDGKEVLNVLETYPRDELFQ
                     ADIPDLIRIVRGVVNLYERRTVRLLVRRDPYQRFYSCLVYVPRDRYNTEVRQRIERIA
                     TEGFAGTSVESNAQISGSSHARVHVVVRTDPARPHKPDFAAIERRIAAAALTWSDRLR
                     DVLTGERGEAAGLALFARYRHAFPMAYQDDVAPETVLADLADLETLRNEPQALRLKLY
                     RPAVQKAARLHLKIVKLADPVPISDILPMLENFGLRVISERPYQLAWPEGGAAWIQDF
                     ELEHRDGAVLDIARVEDKFREAFLAAWSGAIENDGFNRLVLGADLSARQIVVLRACCR
                     YLLQTGVPFSQIYMERTLAGNAAITHNLVRLFETRFDPATAPLPRAQRAADNIVAQIR
                     ASLDAVASLDDDRILRAYLNLVRATLRTNYYQPGSDGKPRSYLSFKLDPAQVPDLPLP
                     RPKFEIFVYSPRVEGVHLRMGYVARGGIRWSDRPQDFRTEILGLVKAQNVKNAVIVPV
                     GAKGG"
BASE COUNT          584 a         1312 c         1253 g          624 t
ORIGIN      
        1 atagatagac acttttctac ctattgcggg gttgagtcat tttcggggta tcacagtcgc
       61 tcagcggggt gctcaacgag cgcttcttta gatcacgccc cggcggatct ggtcctgctc
      121 gatcgactcg aacagggcgc ggaaattccc ctcgccgaac ccctcgttgc ccttcctctg
      181 gatgatctcg aagaagatcg gaccgatcac gttctgggtg aagatctgca gcagcacgcc
      241 ctcgcccttg gcggggttgc catcgatcag gatgcgtcgc tggcgcagct cgccgagcga
      301 ctcgcggtgt ccgggtacgc gcgccgccac tccgtcgtaa tagctgtcgg gcgtgtcctg
      361 gaaggcgacg ccgtgggcac gcagcacgtc cacggtgtgg tagatgtcgg cggtggcgag
      421 cgcgatgtgc tggatgccct cgccgtgata ctcgcgcagg tattcctcga tctggctctt
      481 gtcgtcctgt gactcgttga tcgggatgcg gatcttgccg cagggactcg tcatggcgcg
      541 cgacagcaga cccgtgagct tgccttcgat gtcgaagtag cggatctcgc ggaagttgaa
      601 cagcttctcg tagaacccgg cccacacgtc catgcgcccg cggccgacgt tatgggtgag
      661 atggtcgatg acggtgagcc cggcggcctc ggccggcggc ggcgttgcca ccgggcgaaa
      721 gtccacgtcg tagatcgagc gctcgccgta gagatccacc agatagatca gtgagccgcc
      781 gatgccctcg atgcagggaa tgttcagctc catcggaccg acgctctgtg gcccgggttt
      841 cgcccccagc tcgagcgcgc ggcggaacgc gccggccgcg tcgcgcaccc ggaaggccat
      901 ggcacacgcc gagggtccgt gcaggcgggc aaatccctgt gcgaaggagg caggctcggc
      961 gttgatgatg aagttgatct cgccctgacg gtgcagcgtt acgttcttgg aacggtggcg
     1021 tgctaccacc ggaaatccca gccgctcgaa cagcgtacgc agcaactgcg ggtccggcgc
     1081 cgtatattcg acgaattcga acccgtcggt gcccatcggg ttgtcggtag aagcggtcat
     1141 gatgggcatt ataggcgtcg gtttccgcat gtataatcgc gcgcctccat gcacgcgcgc
     1201 gttgcttccg atgacagccg gctgctgcgc agcatccccg ccgcgcgggt cgcgctaatc
     1261 gagcggatcg cgcgtgcggc gagcggacgc gggagccgca acgagctcca ccagcgcttc
     1321 ctgcgcggct atttccacgg cgtcgcggaa gaggatctgg ccgcgcaccc gccggggcaa
     1381 ctcgccaagg cggcgctcgc ccatctcgcc ctcgggatgc gccgcgctcc gggacgcgcg
     1441 ctggtacggg tcttcaaccc cgtcgcgggc gcggacggct tcgagtcgcc gcacaccctg
     1501 gtgctcacgg tcacggacga catgccgttc ctggtcgact cgctcggcat ggccttgagc
     1561 ggcgccgggc tcgccgtgca cctgatcgtg cacccggtgc tgcaggtgcg ccgcgacggg
     1621 cgcggccgcc tcaccgagat cggcgccaac ggctcgcagg ccgtgcaccc cgagtcgtgg
     1681 cagctgtacg aaatcgaccg cgccaccgac ccgcagcagc tcgagaagct gcggcgcgag
     1741 ctcgagtcca cgctcgccga cgtgcgcgcc gtggtcagcg acttcatgcc gatgcgcgag
     1801 cgcgtgcgcg agatcatcgc gcggctcgag accgacccac cgccgctgcc cgcggccgag
     1861 gtgaacgagg cgcgccatct cctcgactgg atggagggcc ggcacttcgt attcctcggc
     1921 taccgccact accggctcga gcgcggacgc tccgaggacc ggctcatccc cgacgtgctc
     1981 tcgagcctcg gcgtgctcgc gcgcggccgc ggccgcgccg ggcgctctgc cgcgacggtg
     2041 ctgcgcggcg acgtgcgtgc gcgcgcgcgc gatccggagc tcctgatcgt caccaaggcg
     2101 aactcgatcg ccaccgtgca ccgggccgag tacctcgatt acgtcggcat caagaccttc
     2161 gatagccgcg gcctggtgga tggcgagcac cgcttcctcg gcctgtggac ctcgaccgcc
     2221 tatcacggca gcccgcgcga gatcccggtt ctgcgccgca aggtggagcg cgtgatcgag
     2281 cacttcggcc tcgaccccgc gagtcacgac ggcaaggaag tgctcaacgt cctcgagacc
     2341 tacccgcgcg atgagctgtt ccaggcggac atacccgatc tcattcgcat cgtgcgcggc
     2401 gtggtcaacc tgtacgagcg gcgcaccgtg cgcctgctgg tacgccgcga cccctatcag
     2461 cgtttttact cgtgcctggt gtacgtgccg cgcgatcgct acaacaccga ggtgcgccag
     2521 cgcatcgagc gcatcgcgac cgagggcttc gccggcacga gcgtcgagag caacgcacag
     2581 atctcgggct cgagccacgc acgcgtacac gtcgtggtgc gcaccgatcc ggcgcgtccc
     2641 cacaagcccg acttcgccgc catcgagcgg cgcatcgccg cggccgccct cacctggtcg
     2701 gatcggctgc gtgatgtgct gacgggtgag cggggtgagg ccgcggggct cgcgctgttc
     2761 gcgcgctacc gccatgcctt cccgatggcc taccaggacg acgtcgcccc ggagacggtg
     2821 ctcgccgacc tcgcggacct cgagacgctg cgtaacgagc cgcaggcgct gcgcctcaag
     2881 ctctatcggc cggcggtgca gaaggcagcg cgcctgcatc ttaagatcgt caagctcgcc
     2941 gatccggtgc cgatttccga catcctgccg atgctggaga acttcggcct gcgggtgatc
     3001 tccgagcgtc cctaccagct cgcctggccc gagggcggcg ccgcctggat ccaggacttc
     3061 gagctcgagc accgcgacgg cgcagtgctc gacatcgcgc gcgtcgagga caagttccgc
     3121 gaggcgtttc tcgccgcctg gagcggcgcg atcgagaatg acggcttcaa ccgcctggta
     3181 ctgggcgccg acctgagcgc gcgtcagatc gtcgtgctgc gcgcctgctg ccgctacctg
     3241 ctgcagaccg gcgtgccctt cagtcagatc tacatggagc gcacgctcgc gggcaacgcg
     3301 gcgatcacgc acaacctggt gcggctcttc gagacgcgct tcgatcccgc tacggcgccc
     3361 ctgccccgcg cgcagcgtgc cgccgacaac atcgtcgccc agatccgggc gagcctcgat
     3421 gcggtagcga gcctcgatga cgatcggatc ctgcgcgcct atctcaacct ggtgcgggcg
     3481 acgctgcgca ccaactacta ccagccgggc agcgacggca agcccaggag ctacctgtcg
     3541 ttcaagctcg acccggcgca ggtccccgac ctgccgctgc cgaggcccaa gttcgagatc
     3601 ttcgtataca gcccgcgggt cgagggcgtg cacctgcgca tgggctacgt cgcgcgcggc
     3661 ggcatccgct ggtcggaccg gccgcaggat ttccgcaccg aaattctcgg cctggtcaag
     3721 gcgcagaacg tcaagaacgc ggtgatcgtg ccggtcggcg ccaagggcgg ctt
//
LOCUS       DATLKL010000708         2955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_42441, whole genome shotgun sequence.
ACCESSION   DATLKL010000708 DATLKL010000000
VERSION     DATLKL010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42441"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /gene="gspJ"
                     /locus_tag="VM713_09540"
     CDS             <1..498
                     /gene="gspJ"
                     /locus_tag="VM713_09540"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HVH84546.1"
                     /translation="RPVRQPIGDEPAQPALQVSPGESQSSGRVTTLGGTGSTLSSATS
                     GTPPLVALTRAGWANPNGLQRPAEQRVAYFFENGTLKREYWYVLDPTLSSTAASRDLL
                     KKVKSVTFEYLDLTHTWQPQWPPATVAGAQAFESNLRIRPLAVRVTIDTEDFGKIMRI
                     IEVAG"
     gene            498..1487
                     /gene="gspK"
                     /locus_tag="VM713_09545"
     CDS             498..1487
                     /gene="gspK"
                     /locus_tag="VM713_09545"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HVH84547.1"
                     /translation="MRAAVPRRPGGRERPPPPRAQRGIALLVAILLVALGTILAAAIA
                     YENAMTFQRGAATYAFDQALLVQQGAEALAAYGLRLAQKSDPQHTYASEGWAQPVGPF
                     EVVPGVMLSAQLEDLQGRFNLNSLVKQDGTPDPVQVTAFQQLLVMLGLEPKWAGYVVD
                     WIDRDIVPSVPDGAEDSVYMGQTPPYRTPNRYITSASELLALPGFGRDRYVALAPYVT
                     ALPYGVKLNVCTAKPMVLDAFLGHGDFSSNTDGLAKSRASATGCVPSLSDYQASTDPA
                     TWSKISPKLQMTSSYFRLTSLIAIGSTEFNLYSLLLQDPTGQVRPILRSYTAD"
     gene            1503..2735
                     /gene="gspL"
                     /locus_tag="VM713_09550"
     CDS             1503..2735
                     /gene="gspL"
                     /locus_tag="VM713_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HVH84548.1"
                     /translation="MADWLLLRLPRTPDEPATWLIASAAGVPLAATQSGALADAAPAA
                     TGRRVCAIVAGSDVLLAEPELPAKSGIKLQQLVPYALEEQLADNIDELHFALGRRAAD
                     SKRTPVAVVARTLMDEWLAALRAAGLEPECVYADSELLPANPGQAVALLEEDSVCVRA
                     PGAPPVSLPVDALAEALAVAESSAPAAEAAGLILYSGSAAWQRHAVEIEDARGRFASL
                     KVQLLGDGPLALFAQQLPSTAAINLLQGGYAPKRSPAAGLAAWRVAAVLLLALVALHV
                     AGKAAELRVLQSKEHEVDASIRDTFHRALPAEAGTADARRRMEQRLAAVRNGGGSSGL
                     LSALQAVAQARGNAAGTGVQSLNFHDGLLELTLSAPDAASLDHLSQQLRNGGWQADLV
                     GGNTVGGAYQGRVQIRAH"
     gene            2749..>2955
                     /gene="gspM"
                     /locus_tag="VM713_09555"
     CDS             2749..>2955
                     /gene="gspM"
                     /locus_tag="VM713_09555"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HVH84549.1"
                     /translation="MKLSADKPSFEALARWFAALSEREQRIVQIGAALALLLLVVGVV
                     LPLDRSVDQAHARLLKKRTDLAWMQ"
BASE COUNT          446 a         1078 c         1006 g          425 t
ORIGIN      
        1 cgcccagtgc gtcagccgat tggcgacgag cccgcgcagc cggcgctcca ggtgagcccc
       61 ggcgagagcc aatcgtccgg ccgcgtgacc acgctcggcg gcacgggcag cactctgtcg
      121 tcggcgacca gcggcacgcc gccgctcgtc gccctgacgc gcgcgggctg ggcgaatccg
      181 aacggcctgc agcgcccggc cgagcagcgc gtcgcgtact ttttcgagaa cggtacactc
      241 aaacgcgagt actggtatgt gctcgacccg accctctcga gcaccgccgc aagccgcgat
      301 ctgctgaaga aggtgaagtc ggtgaccttc gagtacctcg atctcaccca cacctggcag
      361 ccccagtggc cgcccgcgac cgtcgccggc gcccaggcgt tcgagagcaa cctgcggatc
      421 cggccgctcg ccgtgcgtgt cacgatcgac accgaggact tcggcaagat catgcgcatc
      481 atcgaggttg ccggctgatg cgcgcggccg tgccacgccg cccgggaggg cgcgagcgcc
      541 cgccgccgcc gcgcgcgcag cgcggtatcg cgctgctcgt ggcgatcctc ctggtcgctc
      601 tcggcacgat tctcgccgct gcgattgcct acgagaacgc catgaccttc cagcgcggcg
      661 ctgccaccta cgccttcgat caggcgttgc tggtgcagca gggcgcggag gccctagccg
      721 cgtacggact gcggcttgcg cagaaatccg atccgcagca tacctacgcc tccgagggct
      781 gggcgcagcc ggtcgggccc ttcgaggtcg tgccgggcgt gatgctgagc gcgcagctcg
      841 aggacctgca ggggcgcttc aatctcaaca gtctggtgaa gcaggacggc acgcccgacc
      901 cggtacaggt gacggcattc caacagctgc tcgtcatgct cgggctcgag cccaagtggg
      961 ccggctacgt ggtcgactgg atcgaccgcg atatcgtccc ctcggtcccg gacggcgcgg
     1021 aggacagcgt ctacatgggg cagacgcccc cctaccgcac accgaaccgt tacatcacga
     1081 gtgccagcga gctgctggct ctgccggggt tcgggcgtga tcgctacgtg gcgctcgcgc
     1141 cctacgtcac cgcgctcccc tacggcgtca aactcaacgt ctgcacggcc aaacccatgg
     1201 tgctcgatgc cttcctgggg catggggatt tctcgagcaa caccgacgga ctggccaaga
     1261 gccgcgcgag cgcgaccggc tgcgtccctt ccctgagcga ctaccaggcg agcaccgatc
     1321 cggcgacctg gtcgaaaatc tcgccgaagc tgcagatgac ctcgagttac ttccgcctca
     1381 ccagcctcat cgctattggc agcaccgaat tcaacttgta cagtcttctg ctgcaggatc
     1441 ccacgggtca ggtccgtccc atcctgcgca gctacacagc ggattgaggg gcccgacggc
     1501 acatggctga ctggttgctg ctgcgcctgc cgcgcacgcc ggatgagccg gccacgtggc
     1561 tcatcgcgag cgcggccgga gtgccgctcg cggccacgca aagcggagcg ctcgccgatg
     1621 cggcgcccgc cgccaccgga cgccgcgtgt gcgccatcgt ggccgggagc gatgtgctgc
     1681 tggcggagcc cgagctcccg gcgaagagcg gcatcaagct gcagcagctg gtcccctatg
     1741 cgctcgagga gcagctggcc gacaatatcg acgagctgca cttcgcgctc ggcaggcgcg
     1801 cagccgattc caagcgcaca ccggtggccg tcgtagcgcg cacgctcatg gacgagtggc
     1861 tcgcggcgct gcgcgctgcg ggcctcgagc ccgaatgcgt ctacgccgac agcgagctgc
     1921 tgccggccaa cccgggtcag gcggtggcgc tgctcgagga agacagtgtg tgcgtgcgcg
     1981 cgcccggcgc gccaccggta tcactgccgg tcgacgcgct cgccgaggcg ctcgccgtcg
     2041 ctgagtcgtc cgcaccagcg gccgaagccg ccggcctgat tctctacagc ggcagcgccg
     2101 cgtggcagcg gcatgcggtc gagatcgagg acgcgcgcgg gcgcttcgcc agcctcaagg
     2161 tccagctcct cggcgacggg ccgctcgcgc tctttgcgca gcagctgccc tccaccgcgg
     2221 cgatcaacct gctgcaggga ggctacgcgc cgaagcgctc cccggcggca ggactcgccg
     2281 cctggcgcgt cgcggcagtg ctgctcctgg cgctcgtggc gctgcacgtc gccggcaagg
     2341 cagcggagct gcgcgtgctg caaagcaagg agcacgaggt cgacgcctcg atacgtgaca
     2401 cctttcatcg cgcactgccc gcggaagccg ggacggcgga cgcccgccgg cgcatggagc
     2461 agcgcctcgc cgcggtccgc aacggcggcg gctcgagcgg cctgctcagc gcgctgcagg
     2521 ccgtcgccca ggcgcgcggc aacgcggccg gtaccggcgt gcagtcgctc aactttcacg
     2581 acggcctgct cgagctcacg ctcagcgcgc ccgacgccgc gagcctcgat cacctgagcc
     2641 agcagctgcg taacggcggc tggcaggcgg atctggtcgg cggcaacacc gtcggcggcg
     2701 cctaccaggg gcgcgtgcag atccgcgccc actagaggtg cgcgagcgat gaagctgtcg
     2761 gccgacaagc cgtccttcga ggccctcgcg cgctggttcg cggcgctctc cgagcgcgag
     2821 cagcgcatcg tgcagatcgg cgcggcgctc gccctgctgc tcctcgtggt cggcgtggtg
     2881 ctgccgctcg accgcagcgt cgaccaggcc cacgcgcggc tcctgaagaa gcgcactgac
     2941 ctcgcgtgga tgcag
//
LOCUS       DATLKL010000709         1580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_87533, whole genome shotgun sequence.
ACCESSION   DATLKL010000709 DATLKL010000000
VERSION     DATLKL010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87533"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VM713_09560"
     CDS             <1..260
                     /locus_tag="VM713_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84550.1"
                     /translation="LSASDPEAALLRAGYLPEKDGIQVLRARVRDYEHRRGEFLREAI
                     PWHGAGRIAVKEHVVEGLANAPAHLLLAMRGDNFGRPLVKL"
     gene            complement(992..1336)
                     /locus_tag="VM713_09565"
                     /pseudo
     CDS             complement(992..1336)
                     /locus_tag="VM713_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT          210 a          569 c          531 g          270 t
ORIGIN      
        1 gcctgagcgc aagcgaccct gaggccgcgc tgctgcgcgc cggctacctt ccggagaagg
       61 acgggatcca ggtgctgcgc gcgcgggtcc gcgactatga gcaccgtcgg ggcgagttcc
      121 tgcgcgaggc catcccctgg cacggcgccg gccgcatcgc cgtcaaggag catgtcgtcg
      181 agggcctcgc caacgcccct gcgcacctgc tgctcgcgat gcgcggcgac aacttcggcc
      241 gcccgctcgt caagctctag gaccttccgg cgtggcggaa gcccccgccg attcccagcg
      301 ggggcttctc acgcgttcag cggtggcggc gcgccgggct gcgctttgcg cccttgcggg
      361 cgctcttctt cgccgtcttt gtgcgcgcca cttttttctt cgcggctttc ttctttgcgg
      421 gtttcttctt gccggccttc ttcttcgctg ccttcttttt cgccggcttc gcggccgccg
      481 gctgcggcgc tgcgggtgcg ggtgcagccg cgggtttcgg agcgggagcg gcggccggct
      541 tcggcggcgg cggcgccgca ggctttgcgg cggcggcgcg tggcggctcc tgcaccgcga
      601 acgcaccccc ctgcggatcc atgaagagcg cgatccagct gccgcccgga acctcgaccg
      661 gtccatgcag cagccgtccg ccggccgact tggccgcgct gaccgcgcgg cccgagtccg
      721 ccacctgcac gtagctcagc cacgaaggcg gtgtcgccgg cccctggacg ttgcacatgc
      781 cgccgaccat cgagccgaag cgctcgaaga tctggtagat gcccatggcg cccatgtcgt
      841 ggcccgttcc cttggtccag ccgaacagct cgccgtagaa acgcagcgcg ccggcgacgt
      901 cggtggtcgc cagctcgtgc cacgagaaga caccgggtcc gggcgcaggg ctgctgccgc
      961 cgctcgggcc cggcgtgtag agcgcaaatg tcgcgccctg cggatcggcg agcaccgcga
     1021 agcggccgac gttcgggata tcggacgccg tcttcaccac tcgcgcgccg agacgctggg
     1081 cgtgctcggc cgtggcatcc acattcgggg taccgatgta gatgagccag tgcggtggcg
     1141 ttcctgccgc atcgggaggc aatgccatga ggccgccgac ctggctctgg ccagccatcc
     1201 agatggtgta accgggcatg ctcgacggct gggtccgcca cggcaccact ctggggtaga
     1261 aggcagcggc ggcggccgtg tcagtcgtca acaactcgtg ccagatgaat cgtccccgga
     1321 tatcggcatt gctcatggcc ttttcctctt gtttgcaaac gacctgtcag cgaacgagct
     1381 tccactgtcg aggattagcg ccgcattctg cctcgccgcg cctgtcactg ccatgactcc
     1441 tgatgctgcg cgtgctgcgg cgcgctgcgc gcgtgccaga tgaggtagag cccgctcgcg
     1501 atcacgatgc cgccgcccag gcacacccgc gccgagggca acacctgcca gaccacccgg
     1561 tcgataccga ctccccagag
//
LOCUS       DATLKL010000710         1715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_132810, whole genome shotgun sequence.
ACCESSION   DATLKL010000710 DATLKL010000000
VERSION     DATLKL010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132810"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1715)
                     /locus_tag="VM713_09570"
     CDS             complement(<1..>1715)
                     /locus_tag="VM713_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HVH84551.1"
                     /translation="QLRRSIVDALARAEDDPAVKAVVLIGSGKGFSGGADIREFGTPK
                     SLAAPNLHGVLRAVEDCSKPVIAAIDGVCMGGGFELALACHYRIATSNAQMALPEVKL
                     GLLPGAGGTQRLPRVVGVERALNMIVSGNPVRAEELRGTALFDAMAAGDLRAAALDLA
                     RALIAEGRGPRRVRDLRVQMPNAEAYLQFARNTVRQLAGPYPTPPACVEALAAALKEP
                     FEAGLKRERELFVTLMASPESAALRHIFQAERAASHIDGMPSAVATRPINRVGVVGAG
                     TMGGGITMSFINAGLPVTLLETTQEALDRGLTNIRRNYQGALRKGSLTEASLERRLAL
                     ITPTLQYEPLHDADLVVEAVFENLEVKRKVFERLDEIIKRGAILASNTSALNLDVIAR
                     FTKRPQDVIGLHFFSPANVMRLLEVVRGAETANDTLATAMALARTIGKIAVVSGVCDG
                     FIGNRIVARYGMAAHDLLVAGALPQQIDAALQNFGMAMGPFRMADLAGLDIGWAIRKR
                     RAAESPNEDFSNVADILCEAGRFGQKTGSGFYRYEAGAREAIPDPAVTAIIERYRASR
                     GVVART"
BASE COUNT          284 a          597 c          554 g          280 t
ORIGIN      
        1 gtacgcgcta ctacgcctct actagcgcgg tatcgttcga tgatcgcagt caccgccgga
       61 tcgggtatcg cctcacgtgc gccggcctcg tagcggtaga accccgagcc ggtcttttgc
      121 ccgaagcgac ccgcctcgca gagaatatcc gcaacatttg agaaatcctc gttgggagat
      181 tccgccgcgc ggcgcttgcg aatcgcccag ccgatgtcga gtccggcgag atccgccatg
      241 cggaacgggc ccatcgccat cccgaagttc tgcagcgccg cgtcgatctg ctgcggcagc
      301 gcgcctgcga cgagcaggtc gtgcgccgcc atgccgtagc gcgccacgat ccgattgccg
      361 atgaaaccgt cgcaaacgcc cgatacgacc gcgatcttgc cgatcgtcct ggccaatgcc
      421 atggccgtgg cgagggtgtc gttcgcggtt tctgctcctc gcacgacctc gagcagccgc
      481 atgacgttcg cgggactgaa gaagtgcagg ccgatgacgt cctgcggccg cttcgtgaac
      541 cgggcgatga catcgaggtt cagggccgag gtgttggacg cgagaatcgc gccgcgtttg
      601 atgatctcgt cgagcctttc gaacaccttc cgtttgactt cgagattctc gaacacggcc
      661 tcgaccacca ggtccgcatc gtgcagaggc tcgtactgca aggtcggtgt gatcagcgcc
      721 aggcgccgct cgaggctcgc ctcggtgagc gaccccttgc gcagcgcgcc ctggtaattg
      781 cggcggatgt tggtaaggcc ccggtcgagc gcctcctgcg tcgtctccag gagcgtgacc
      841 ggcaatcccg cattgatgaa gctcatcgta atgccgccac ccatcgtgcc tgcgccgacg
      901 acaccgaccc gattgatcgg gcgcgtcgcg accgcactgg gcatgccgtc gatgtgggaa
      961 gcggcgcgct cggcttgaaa gatgtgccgc agcgccgccg attcagggga agccatgagc
     1021 gtcacgaaca gctcgcgttc gcgcttcaac ccggcctcga agggctcctt gagcgcagcg
     1081 gcgagcgcct ccacacaggc cggcggcgtc gggtaggggc cggcgagctg cctcaccgta
     1141 ttgcgggcga attgcaggta ggcctcggcg ttgggcatct ggaccctgag gtcgcgcacc
     1201 cgcctcgggc cccgcccttc cgcgatcagc gcgcgtgcca gatcgagcgc ggccgcgcgc
     1261 agatccccgg cagccatggc atcgaacagc gccgtgccgc gcagctcctc ggcgcgcacc
     1321 ggattgccgg acacgatcat gttcagcgcc cgctcgaccc cgaccacgcg cggcagcctc
     1381 tgagtgccgc cagccccggg caggagaccg agcttcacct ccggaagcgc catctgcgca
     1441 ttcgaggtgg cgatgcggta gtgacaggcg agcgccagct caaacccccc gcccatacac
     1501 acgccatcga tggccgcgat caccggcttg ctacagtcct cgaccgcgcg cagtacgccg
     1561 tggaggttcg gggcagcgag tgacttcggc gtgccgaatt cacggatatc ggcgccgccg
     1621 gagaagccct tacctgaacc gatgaggacg acggccttca cagccgggtc gtcctcggcg
     1681 cgcgccagcg cgtccacgat cgaccggcgc agctg
//
LOCUS       DATLKL010000711         2910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_110058, whole genome shotgun sequence.
ACCESSION   DATLKL010000711 DATLKL010000000
VERSION     DATLKL010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110058"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VM713_09575"
     CDS             complement(<1..339)
                     /locus_tag="VM713_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVH84552.1"
                     /translation="MASDPRAKERRAAEGTLRASEACYRAIFEQAAVGIVHTSLEGKI
                     RLVNPAFCAMTGFSDVEARQLHIREITHSEDIHASILSRAALVERGEAPYQREIRLRR
                     KNGSYLWASVS"
     gene            complement(554..1207)
                     /locus_tag="VM713_09580"
     CDS             complement(554..1207)
                     /locus_tag="VM713_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVH84553.1"
                     /translation="MARIRVMLADDHRLVRAGIRALLQTVKEVDVVAEAGNGREALQL
                     VAAHHPDVVLMDIMMPSLNGLDAAARITRTFPRTRVVILSMNPDEDSVLKAIRAGAVG
                     YLVKSADPAELELAVRAVARGETFLSSAVAAHVLAACLNRVDKEQTSIGRLTLRQREV
                     LQLIAEGHTTKEIATKLAISTKTAEAYRADLMKALDIHDIATLTRYAIRTGLVSLDP"
     gene            complement(1212..>2910)
                     /locus_tag="VM713_09585"
     CDS             complement(1212..>2910)
                     /locus_tag="VM713_09585"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVH84554.1"
                     /translation="SFWDNASPDSREVVRAREAARQRGDPVPPRFELKILTRAGEERW
                     LDFTEAAFEFEGEPAELGIALDVTERKHAEEALRKSERVLREAEGLGHTGSWDHDLVK
                     GIIFNTPENLRLFFGDDRKTAGFEDFVAAIHPDDRAYGLAQHEKLVSEGSPIELEFRV
                     VWPDGTVRMLLGRAAVVRDAAGRIVRAYGTNLDVTDRKQTEKAMRDTQELLNQVLATI
                     PVGVAVMNKEGDFILGNAALKRIWGAAPVVRGEQRWAQSKGWWHHSGKRILPTEWASV
                     RALSQEQTSLNELIDIENFTGQHKTIQNSAAPIRNTEGSIVGAVVVNEDVTERVQAED
                     ALQDSADRLQHLSRRLLAVQEEERRHLSRELHDEFGQLLFSINLHLQAAKKAAGRGAQ
                     PNINESITLLQRAGTQVRSLALELRPMLLETAGLDATLRWLAQQYEQRSGVDIEVVGH
                     VTEVPGEVAIACFRVVQEALTNVVRHAAARRVAIELSQREGLLRLVIRDDGAGFDVSR
                     TLDGAAAGGHLGLVGMRERVEILRGSLTIDSGRGRGTRIDVSFPLTDPAAVPTPQTA"
BASE COUNT          457 a          972 c          958 g          523 t
ORIGIN      
        1 cgagacgctt gcccagaggt aggagccgtt cttgcgacgc agccggatct ccctttgata
       61 gggcgcctcg cctcgctcca ccagggccgc gcggctcagg atgctggcgt ggatgtcttc
      121 ggaatgggtg atttctcgga tgtggagctg tcgtgcctcg acgtcggaga atcccgtcat
      181 ggcacagaac gccgggttaa cgaggcggat tttcccctcg agcgacgtgt ggacgatgcc
      241 caccgcggcc tgctcaaaga ttgcgcgata gcaggcttcg cttgcgcgca acgtcccctc
      301 cgccgcgcgc cgttccttgg cgcgcgggtc agacgccatg catgcgacag gggagccatg
      361 gcgatcgcat cgggggcccg attgaggcca gggccgacgt tatcgacagc gcggggtcga
      421 ggcgaattgc cacaacccta gtgttgccga gccgccggcc gcgttcgacc cggtgaaacc
      481 agggaaaggc ggggggaaac tatgggctag agggtcgccg cagccacacg gcccggcgcg
      541 ctgcggggcg gactcacgga tcgagagaga cgagacccgt gcggatcgcg tagcgcgtca
      601 gcgtggcgat gtcatggatg tccagggcct tcatgagatc ggcccggtaa gcctcggccg
      661 tcttggtgct gattgcgagc ttggtggcga tctctttcgt cgtgtgcccc tcagcgatca
      721 gctgcagcac ctcgcgctgg cgcagtgtca gtcgcccaat cgacgtctgc tccttgtcga
      781 cacgattgag gcaagcggcg agcacgtgcg ccgccaccgc agagctcagg aatgtctcac
      841 cccgcgcaac cgctcgcacg gccagctcca gctccgcggg atcggccgat ttgaccaggt
      901 agccgaccgc gccggcgcgg atggccttga gaaccgaatc ctcgtcgggg ttcatggaga
      961 ggatgacgac acgagtgcga ggaaaggtcc gggtgatgcg cgcggccgcg tcgagtccgt
     1021 tgagacttgg catcatgatg tccatgagca ccacatcagg gtggtgagcc gcgaccagct
     1081 gcagcgcctc acgcccgttc ccggcctctg caaccacgtc gacctccttg acggtctgca
     1141 gcaacgcgcg aatgccggcc cggacgagcc tgtggtcatc ggcgagcatg acgcggattc
     1201 tggccatgcg cttatgccgt ttgcggtgtc ggcacagccg ctgggtcggt gagagggaag
     1261 gacacatcga tccgagtgcc acgccctcgg ccagagtcga tcgtgagact cccgcggaga
     1321 atctcgacgc gctcgcgcat tccaaccaat ccgaggtggc cgccggccgc ggcgccgtcc
     1381 agagtccgtg aaacgtcgaa tcccgcgcca tcgtcgcgga tcacgagtct gaggagtccc
     1441 tcgcgctggc tcagctcgat cgctacgcgt cgcgcagccg catgtcgcac cacattggtg
     1501 agcgcctcct gcacgacgcg aaagcaggcg atcgcaacct cgcccggtac ttcggtgacg
     1561 tggccgacga cctcgatatc gacgccgctg cgctgctcgt actgctgcgc cagccagcgc
     1621 agcgtcgcgt ccagccccgc ggtctcgagc agcatgggcc gcagctcgag cgcgaggctg
     1681 cgcacctgcg tgcctgcccg ttgcagcaga gtgatgcttt cgttgatgtt cggctgcgcc
     1741 ccgcgtcctg ccgccttctt agccgcctgc aggtgcaggt tgatgctgaa gagcagctgg
     1801 ccgaattcat cgtgcagctc gcgcgacagg tgacgccgtt cctcctcctg tacggcgagc
     1861 agccggcgcg agagatgttg caggcgatcc gcagagtcct gcagggcgtc ctcggcctgt
     1921 acccgctcgg taacgtcctc attgacgacc accgccccca cgatactgcc ctcggtgttg
     1981 cggataggag cggcggagtt ctggatcgtc ttgtgttgac ccgtaaaatt ctcgatatcg
     2041 atgagctcat tgagactcgt ctgctcctgc gacagtgccc gcactgaggc ccactccgtg
     2101 ggcagaatcc tctttccgga gtggtgccac cagcccttgg actgcgccca ccgctgctcg
     2161 ccgcgcacaa cgggggccgc gccccagatg cgcttcaggg cggcattgcc gaggatgaag
     2221 tcgccctcct tattcatgac ggccaccccc acggggattg tcgcgagcac ctggttgagc
     2281 agctcctgag tgtcgcgcat ggccttttcc gtctgcttgc ggtccgtcac gtcgaggttg
     2341 gtcccgtagg cacggacgat tcgccccgca gcgtctcgaa ccaccgcggc gcggccgagg
     2401 agcatgcgaa ccgtgccgtc gggccagacc acccggaact cgagctcgat cgggctgcct
     2461 tccgacacga gcttttcgtg ctgcgccagt ccgtaggcgc ggtcgtcggg atggatcgcc
     2521 gcgacgaagt cttcgaagcc tgcggtcttg cggtcgtcgc cgaagaacag gcgcaggttc
     2581 tccggggtgt tgaaaattat gcccttgacg agatcgtggt cccagctgcc ggtgtgcccg
     2641 agcccctcgg cctcgcgcag cacgcgctcg ctcttgcgga gtgcctcctc ggcgtgcttg
     2701 cgttcagtga cgtcgagggc gatgccgagc tcggcgggtt cgccctcgaa ctcgaaggcc
     2761 gcctcggtga agtccagcca gcgttcctcg cccgccctgg tcaggatctt cagctcgaag
     2821 cgcggcggca ccgggtctcc cctttggcgc gccgcctcgc gcgcccggac cacctcccgg
     2881 gaatccggcg aagcgttgtc ccagaagctt
//
LOCUS       DATLKL010000712         1515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_166587, whole genome shotgun sequence.
ACCESSION   DATLKL010000712 DATLKL010000000
VERSION     DATLKL010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166587"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="VM713_09590"
     CDS             complement(<1..1265)
                     /locus_tag="VM713_09590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH84555.1"
                     /translation="MSLRSAQIRTLVLLFCGYAACYYCRADLSVETPLLADELARKGM
                     SHGEALIHIGTITSIGVFAYALGKLFLTGLGDYWGGRRNFFIGVGGATVFTLLFAAGA
                     TLPVFTIAWTANRLTQSVGWAGLIKVSSKWFNYSSYGLVAGILSLSYLAGDAAARQQM
                     SVLIAHGYGWRALFVFAAVVAGLALLASLLWLRESRVAEGFAEAEPNPLSLFAASESR
                     PASVAALLMPLLRSPAFLLVCLLSFGTTIIRETFNTWTPAYLRDYLGYSVSDSAAISA
                     LFPFVGILSVLLAGWLSDRLGLSGRSLILFLGLAATAAALVALTAVPAGHALSWAAVA
                     LIGVVAFALLGPYSYLGGAFALDFGGKQASAASSGIIDGIGYLGGVLAGYGMARLAVA
                     YGWRGVFVTLAAVSAAAAVSAGYLHQLNLR"
BASE COUNT          291 a          506 c          534 g          184 t
ORIGIN      
        1 cggagattca gctgatggag gtaaccggcg ctcacggccg ccgcggcgct cacggccgcg
       61 agcgtcacga acacgccgcg ccatccatac gcgactgcca atcgtgccat gccgtagccc
      121 gcgaggacgc cccccaggta gccgatgccg tcgatgatgc ccgaggaggc cgcgctcgcc
      181 tgcttgccgc cgaagtcgag cgcgaaggcg ccgccgagat aggagtaggg tccgaggagg
      241 gcgaacgcta cgacgccgat cagagccacc gccgcccagg acagcgcatg gccggccggc
      301 accgcagtga gcgctaccag tgcggcggcg gttgccgcga gcccgaggaa caggatcagc
      361 gagcgcccgc tcaaccccag gcggtcgctc agccagcccg ccagcagcac ggacagaatg
      421 ccgacgaatg gaaagagcgc gctgatcgcg gcggagtcgc tcacgctgta acccaggtaa
      481 tcgcgcaggt aggcgggcgt ccaggtgttg aacgtctcgc ggattatggt cgtgccgaag
      541 gacagcaggc aaaccaggag gaacgcgggg ctgcgcagca gtggcatgag gagcgctgcc
      601 acgctcgccg ggcgcgactc actcgccgcg aacaggctga gaggattggg ctcggcttcc
      661 gcaaagccct cggcgacgcg cgattcgcgc agccacagca ggctcgcgag cagcgcgaga
      721 cccgcgacga ccgccgcgaa cacgaacagc gcgcgccacc catagccgtg cgcgatgagc
      781 acgctcatct gctgccgcgc ggccgcgtcc ccggcgagat aactcaggct caggatcccg
      841 gcgaccagtc cgtaagagga gtagttgaac cacttcgagg agaccttgat gaggcccgcc
      901 cagccgaccg actgcgtcag gcggttggcg gtccaagcga tcgtgaagac ggggagtgtg
      961 gccccggcgg cgaacagcag cgtgaacaca gtggcgcccc ctacgccgat gaagaagttg
     1021 cggcgtccgc cccagtaatc acccagcccg gtgaggaaca gtttgccgag cgcgtaggcg
     1081 aacacgccga tcgaggtgat cgtgccgatg tggatgagcg cctcgccgtg gctcatgccc
     1141 ttgcgggcga gctcatcggc gagcagcggt gtctccaccg agagatccgc gcggcagtag
     1201 taacaggccg cgtagccgca gaacagcagc accagggttc gtatctgagc cgagcgcagc
     1261 gacatgcctg gagcactggc tcgcagcgcc gcatccgcgg gcggggcagg ggtgtcagtc
     1321 ttggtgccga ctctcaaacg ggtcctcgcg gtgcgtcgcg gaggacccga tactagcgga
     1381 ggcggcggtt ggattcacgc ggcggccgca cgcactcgga caagccgccc gccgcctcgg
     1441 caggttcgca aaaatcagcg gtaccagtag ccgcggtagt acccgtgcac cacgacgacg
     1501 ggggcgggcg cgacc
//
LOCUS       DATLKL010000713         1827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_177760, whole genome shotgun sequence.
ACCESSION   DATLKL010000713 DATLKL010000000
VERSION     DATLKL010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177760"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VM713_09595"
     CDS             complement(<1..213)
                     /locus_tag="VM713_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84556.1"
                     /translation="MLRTLENLLNRGLPRSPRARELCAELAGRSLAIEIRDVARLRVS
                     SNGLVLTVSHDGEPADATVSGGPLGLA"
     gene            complement(248..997)
                     /gene="ubiE"
                     /locus_tag="VM713_09600"
     CDS             complement(248..997)
                     /gene="ubiE"
                     /locus_tag="VM713_09600"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008080626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HVH84557.1"
                     /translation="MADQRSTDFGFQRVPLEDKARRVRAVFDSVADRYDLMNDLMSAG
                     THRIWKRFTLALANLRPGQRALDVAGGSGDLAAGLARQVGERGLVVLTDINAAMLERG
                     RDRLIDAGFAANVACVQADAERLPFADGTFDCVTIGFGLRNVTDKAAALASMRRTLKP
                     GGQLLVLEFSQPQLPALKGLYDAYSFRVLPLLGRYVAGDEASYRYLAESIRMHPDQET
                     LLAMMRAAGLEGCRYHNLSGGIVAVHRGYRY"
     gene            complement(1191..1526)
                     /locus_tag="VM713_09605"
     CDS             complement(1191..1526)
                     /locus_tag="VM713_09605"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HVH84558.1"
                     /translation="MPTEIRLRRSSRVLEVTFDDGSRFELPFEYLRVHSPSAEVQGHG
                     PGQQTLVLGKENVQVTAVEPVGQYAVKLVFDDGHDTGLYTWKYLHELGRERATKWARY
                     EERRARAPR"
BASE COUNT          291 a          626 c          629 g          281 t
ORIGIN      
        1 cgcgaggccg agcgggccgc cgcttacggt cgcgtccgcg ggctccccgt cgtgcgacac
       61 ggtgaggacg aggccgttcg agctcacgcg cagccgcgcg acgtcgcgga tctcgatcgc
      121 gagactccgg ccggcgagct cggcgcacag ctcccgggcg cgcggcgagc gcggcaggcc
      181 gcggttcagg aggttctcga gagtgcgcag catcgtggcg tttcgcggcg gcaccggggc
      241 ggcgccttca gtagcggtag ccgcggtgca cggcgacgat gccgccggag aggttgtgat
      301 agcggcagcc ctcgagcccc gccgcgcgca tcatcgcgag cagcgtctcc tggtcgggat
      361 gcatgcggat cgattcggcg aggtagcggt agctcgcctc atcgcccgcc acgtagcgcc
      421 cgagcagcgg cagcacgcgg aaagagtagg cgtcgtagag gcccttgagg gcggggagct
      481 gcggctgcga aaactcgagc accagcaact gcccaccggg cttgagcgtg cggcgcatgg
      541 aggcgagcgc ggccgccttg tcggtcacgt tgcgcagtcc aaagccgatg gtgacgcagt
      601 cgaaggtgcc gtcggcgaac ggcagccgct cggcgtcggc ctggacgcag gcaacgttcg
      661 cggcgaagcc ggcatcgatc agccggtcgc gcccgcgctc gagcatggcg gcgttgatgt
      721 cggtgagcac gaccagcccg cgttcgccca cctgccgcgc cagccccgcc gccaggtcgc
      781 cgctgccgcc cgccacgtca agggcgcgct gcccaggacg cagattcgcc agtgccagcg
      841 tgaagcgctt ccagatgcgg tgcgtcccgg ccgacatcag gtcgttcatg aggtcgtagc
      901 ggtccgcgac cgagtcgaag acggcgcgca cgcggcgtgc cttgtcctcg agcggcacgc
      961 gctgaaagcc gaaatcagtg gagcgctggt cggccatggt ctgcgcggag gtgattgatg
     1021 agacacattc tagtcgatgc agccgagacc tcgctttgca tggcttgtat cttcggcgcg
     1081 ccgatgcgct agaatcgcgc ccgttcaggg gggtcttgca gcacggcggg ccggacagca
     1141 atgtccggcc cgtcttcttt atgcatcacg caggcaccgg ggcgccgcgc tcagcgcggc
     1201 gcgcgcgcgc gccgctcctc gtagcgcgcc cacttcgtgg cacgctcgcg gccgagctcg
     1261 tgcaggtact tccaggtgta gagcccggtg tcgtgcccgt cgtcgaagac cagcttcacg
     1321 gcgtactgtc cgaccggctc gaccgccgtg acctgcacgt tctccttgcc gagcaccagt
     1381 gtttgctgcc ccgggccgtg accctggacc tccgcggacg gggagtgcac gcgcaggtac
     1441 tcgaagggca gctcgaagcg gctgccgtca tcaaaggtca cctccagcac gcgcgagctg
     1501 cgccgcagtc ggatctcggt cggcaccggc tcggacatag ccagcccagt ataggacccg
     1561 cactggacga gcggattgtt gcagcgcaat atattactcg acagccccgc gcggcgagac
     1621 gttccgcgca ttaacgccga tgacaaaccg tagcgcgccc ctatactcac catccctgca
     1681 tgctgcccga gggctcgagg gccttaaggc ccgagcactg aatggaaccg accgatacct
     1741 cgcatccgga ctattaccat cgagttgtgt attgccagtg ggcctgcccg gcccacaccg
     1801 atgtccccga atacatccga ctgatcg
//
LOCUS       DATLKL010000714         1784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_110086, whole genome shotgun sequence.
ACCESSION   DATLKL010000714 DATLKL010000000
VERSION     DATLKL010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110086"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VM713_09610"
     CDS             <1..1140
                     /locus_tag="VM713_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVH84559.1"
                     /translation="EPSATRAGVRHASTGSPSSITTHAYLRVNTIFEGVHAHGGRTAW
                     TDKHPSYEWTNGPSGQGVDDFYGPEINSIPVALPYPGCTPVPYSDPAPDDGWTTSFAN
                     IMCYDSTHVQAVLNQIDGYTHDRANKVGVPALFGTNFQAVSVGEKLRTDPVTGVSGGY
                     SDVLATPGPGLAAELDFVDQSLGQMVQELKSQGVYDSTLIVVGAKHGQSPIDVSKRVG
                     IGGGQPATLIGAAEAFDISDDGSLIWLTDPSLAAGVVQLLSQPANQALLGVQEILAGN
                     LLRDKFNSPATDPRTPDIILKVNTGVIFTGGSKIAEHGGFNEDDVHTALLVSNPRLKA
                     SSVNIAVSNQQVAATIVKALGADPNELQAVRREQIHVLPSLFRGD"
     gene            complement(1219..>1784)
                     /locus_tag="VM713_09615"
     CDS             complement(1219..>1784)
                     /locus_tag="VM713_09615"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVH84560.1"
                     /translation="RPSASGCSLAAVLGRAPPPIYNSGPCTARRAVMAVRPPVPPFTV
                     ESATQKVRGAEDAWNTRDPDRVVQVYTEDTQWRNRSEFPVGREQVRAFLQRKWAKELD
                     YRLIKELWACAGNRIAVRFAYEWHDDSGNWFRSYGNENWEFTEEGLMRRRFASINDLP
                     IAEKERKFRWPLGRRPDDHPGLSDCGL"
BASE COUNT          302 a          611 c          580 g          291 t
ORIGIN      
        1 gagccctcgg caaccagggc gggcgtcagg cacgcgagca ccggctcgcc gtccagcatc
       61 acgacgcacg cctatctgcg cgtcaacacc attttcgaag gggtgcatgc gcatggtggc
      121 cgtaccgcct ggacggataa gcatccttcc tacgagtgga ccaatggccc gagcggtcaa
      181 ggcgtcgatg acttctacgg tccggagatc aattcgattc cggtcgcgct gccttatccg
      241 ggctgcaccc ccgtgccgta ctccgatccg gcgcccgatg acggctggac gacgagcttt
      301 gccaacatca tgtgctatga ctctacgcac gtccaagcgg tgctcaacca gatcgacggc
      361 tacacgcacg accgcgccaa taaggtgggc gtgccggcac tgtttggcac gaatttccag
      421 gccgtgagcg tcggcgagaa gctcaggacg gacccggtca ccggtgtgag cggcgggtac
      481 agcgatgtcc tcgcgacacc cggccccggc ctcgccgcgg agctcgactt cgtcgaccag
      541 tcgctcggcc agatggtcca ggagctgaag agccagggtg tctacgactc gacgttgatc
      601 gtcgtcggcg cgaagcacgg ccagtcgccg atcgatgtca gcaagcgagt cggcattggt
      661 ggtggccagc ccgcaacgct gattggcgcg gcggaggcct tcgacatctc cgatgacggc
      721 tcgctgatct ggctgaccga cccctcgctc gctgcgggcg tcgtgcagct cctcagccag
      781 cccgcaaatc aggcgctact cggcgtccag gagatcttgg cgggtaacct gctgcgcgac
      841 aagttcaaca gcccggccac ggatccgcgc acgcccgaca tcatcctgaa ggtcaacacg
      901 ggagtgatct tcaccggcgg ctcgaagatc gccgagcatg gcggcttcaa cgaggatgac
      961 gtacacaccg cgctgctggt ctcgaacccg cgcctcaagg cgtcctccgt caacatcgcg
     1021 gtcagcaatc agcaggtcgc ggccacgatc gtcaaggcct tgggcgccga tcccaacgag
     1081 ctgcaggcgg tacggaggga gcagatccac gtgctgccgt ccctgttccg cggagattga
     1141 gaacatcgag cccgccgggc cgaaggagcc cggcgggctc cgggccggcg gtggcccctg
     1201 ccccgcgcgg gcgcccggtc acaggccgca gtcgctcagt cccgggtgat catcaggccg
     1261 gcggccgagc ggccagcgaa acttccgctc cttctcggca atcggcagat cgttgatgct
     1321 ggcgaagcgg cgccgcatca ggccctcttc ggtgaactcc cagttctcgt tgccgtagct
     1381 gcggaaccag ttgccggaat cgtcgtgcca ctcataggcg aagcgcaccg cgatgcggtt
     1441 gccggcgcag gcccacagct ccttgatgag ccggtaatcg agctccttgg cccacttgcg
     1501 ctgcaggaac gcccgcacct gctcacggcc cacggggaac tccgagcgat tccgccactg
     1561 ggtgtcctcg gtgtagacct gcacgacccg gtccgggtcg cgggtattcc aggcgtcctc
     1621 agccccgcgc accttttgcg tggcggactc gactgtaaac ggcggaacgg gcggacgcac
     1681 tgccatgacg gctctcctcg cggtgcaggg gccgctattg tagatcggcg gcggtgcccg
     1741 cccgagtacg gcggcgagcg agcagccgct cgcgctcggc cttg
//
LOCUS       DATLKL010000715         5429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_155648, whole genome shotgun sequence.
ACCESSION   DATLKL010000715 DATLKL010000000
VERSION     DATLKL010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155648"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VM713_09620"
     CDS             <1..805
                     /locus_tag="VM713_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVH84561.1"
                     /translation="GPASPDPATGQPYRLTFPDLSVEDIARGGYETVRSLGIERLDTV
                     MGPSLGGMVVLAYAAMFPTAARRLVCISGTMAAAPFAIALRSIQREVITKDPDWRGGN
                     YPADRPPITGQRIARKLGTVTYRSAAEWQQRFGRSPIRADMESKDPFASEFAVQGYLE
                     SLAQRWVNVFDANCYLYISRSMDRFDLAIHGDPVGLFRHAALKSALVIGVQTDLLFSV
                     TEQQAVAQALEAAGVATRFSPLACIEGHDAFLVDLGTFGAEIGAFLRAG"
     gene            1003..1272
                     /locus_tag="VM713_09625"
     CDS             1003..1272
                     /locus_tag="VM713_09625"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVH84562.1"
                     /translation="MHTRHRARTIVYITVPGGRRKLCKAVNLSATGVFIETSNLGLRK
                     GQQVVLSFAINLGTIIKLHRRTAVVAHVSRGGTGLMMETYAQASN"
     gene            1337..1654
                     /locus_tag="VM713_09630"
     CDS             1337..1654
                     /locus_tag="VM713_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HVH84563.1"
                     /translation="MSQLKSLARRRLLQRLALGVSLAALLPPRAHAAAAGPLLEVASP
                     EAKAVKYVEDAATANGATAGSTCANCALYQGADGSKQGPCQIFPGKEVKAAGWCTSWA
                     PQM"
     gene            1877..2845
                     /locus_tag="VM713_09635"
     CDS             1877..2845
                     /locus_tag="VM713_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVH84564.1"
                     /translation="MNQPLLDRRQFATRCAAFGLALPAMLATTARAQAPGTAPAANTA
                     KPAERTVKLPDGTAVTALGQGCWHLGQGRHPAAVEEEALRTGISLGMTLLDTSGNYGH
                     GRSEEFLRHVLAGERERIFLVSKVEGDEISGDGIARACAASLARLGTDYLDLYLLHWP
                     VASAQFAGVVAGFERLRAARKIRAWGVSNFNVGQMEDLLRVPDGHRCATNQVPYSLNN
                     RHIESDVLPWSRQHNMPVMAYSPLGGDNHLVVGERTLAQIGAAHGCSAAAVALAWAIR
                     GGNVIAIPESGSPAHVRENAAALSIALTPQDLQTLNAAYPGPAGTS"
     gene            complement(2851..3198)
                     /locus_tag="VM713_09640"
     CDS             complement(2851..3198)
                     /locus_tag="VM713_09640"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVH84565.1"
                     /translation="MSYMLLIVEPTGQRHDRGREAGQVAYDQMLEYAQSLKARGVLLA
                     TNSLKSAGARLTVRGGKRTVVDGPFTEAKELVGGFFLLNVSSLEAALAFAGECPAAGW
                     ATIEVREIGPCYE"
     gene            complement(3210..4520)
                     /locus_tag="VM713_09645"
     CDS             complement(3210..4520)
                     /locus_tag="VM713_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVH84566.1"
                     /translation="MILACAVQLAVMSSLAMKATEVQRTIDAVWRIESAKLIGALARM
                     TRDVGVAEELAQDALVTALEQSPGSGIPDNPGAWLMATARNRAIDRLRHQRMRAGKHE
                     ALSAELEMDQQHRDGVETAADDEIGDDLLRLIFTACHPVLPTEGRTALTLRLLGGLTT
                     AEIARAFLVPEPTIAQRIVRAKRTLSEAGVPFEIPRGEELGRRLSSVLEVIYLVFNEG
                     YAATAGSDWMRPALCEDALRIGRILAELAPGESEVHGLVALMEIQASRIRARTNARGE
                     PVLLLEQNRALWDRVLLHRGLMALGRGEVLGETRGPYLLQAAIAACHARAATAADTDW
                     VYITALYDELVRLTPTPVVELNRAVAVSMAYGPAAGLELADRLAAEGTLKSYHLLPSV
                     RGDLLAKLGRYAEARGEFERAAQLTRNARESRLLLDRAAACVRS"
     gene            complement(4521..4967)
                     /locus_tag="VM713_09650"
     CDS             complement(4521..4967)
                     /locus_tag="VM713_09650"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVH84567.1"
                     /translation="MPTTLTHLHFRGNCSEAFRFYAATFGGRIVFSMTYGEAPGAAPA
                     APEVRDQIIHARVDLGDDALLGCDVPPDRYEKPQGFNVMANATSPGDAERLFRTLSEG
                     GTVDMPIQETFWAWRFGMCTDRFGTPWMVNCAKPPEALAAAARKSA"
     gene            complement(4973..>5429)
                     /locus_tag="VM713_09655"
     CDS             complement(4973..>5429)
                     /locus_tag="VM713_09655"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVH84568.1"
                     /translation="APATHTSFKETCMRVMVIVKATRDSEAGVLPSEELLTAMGKFNE
                     QLVKAGVLLAAEGLQASSKGVRVQFGGGKSTVSDGPFTETRELIAGFWLWQVRSIDEA
                     VEWIRRSPFQKSNEQIEIRRVFEAEDFGPALTPELRAQQQSRAERISRN"
BASE COUNT          848 a         1855 c         1825 g          901 t
ORIGIN      
        1 ggggcccgcc tcgccggatc cggccacggg gcagccctac cggctgacct tcccggacct
       61 ttcggtggaa gacatcgctc gcggcggcta tgagaccgtg cgcagtctcg gcatcgagcg
      121 gctcgatacc gtcatgggtc cctccctcgg gggcatggtc gtgctggctt atgcggccat
      181 gtttcccacc gcggcgcggc gtctcgtgtg catctcggga accatggccg cggcgccctt
      241 tgccatcgcg ctgcgctcca tccagcgcga agtgattacc aaggatcccg attggcgggg
      301 cggcaattac ccggcggacc ggccgccgat caccggccag cgcatcgccc gcaagctcgg
      361 cacggtcacc tatcgctcgg cggccgaatg gcagcagcgc tttggccgct cgcccatccg
      421 cgcggacatg gagtcgaagg accccttcgc ctcggagttc gccgttcagg ggtacctcga
      481 gtccctcgcc caacgctggg tcaacgtctt cgatgcgaac tgctatctct atatctcccg
      541 gtccatggac cgattcgatc tggccatcca tggggatccg gtgggactat tccgccacgc
      601 cgcccttaag tccgcgctgg taatcggcgt tcaaaccgac ctactgttct ccgtgaccga
      661 gcaacaggcg gtcgcccagg cgctcgaggc cgccggagtc gccacccgct tctcgcccct
      721 ggcctgcatc gaggggcacg acgcctttct ggtcgatctc gggactttcg gggccgaaat
      781 cggggcgttt ctgcgcgccg gctaggccgg cagcgggtgc tgcagcaggg cgggagtgtt
      841 tttttggtcc gcgaccttgc tacagggtga caaaattaag tttactcggc agtcgcggac
      901 cgcagacact gggcataatg gtgcccgggc ctgcggatgg aattgacggg ccttcgaaaa
      961 gagaacaacg aaattgaaat gaaggactct gtggagcgtc gcttgcacac tcgacatcgg
     1021 gctcggacga tcgtctacat caccgtgccc ggcggccgcc gcaaactctg caaggcggtg
     1081 aacctgagcg ccactggtgt gttcatcgag accagtaatc tcgggctgcg caagggacag
     1141 caggtcgtgc tctccttcgc catcaatctc ggcacgatca tcaagctcca tcggcgcacc
     1201 gccgtggtcg cgcacgtctc gcgcggcggc accggcctca tgatggagac ctacgcgcaa
     1261 gcctcgaact gaggcgtccc ggctgacaga tcgccgcgtc gcgggagtgc taggctcttg
     1321 cgctcaagga gatcgcatgt cgcagctgaa atccttagcc cgccggcgcc tgctgcaaag
     1381 actcgctctc ggtgtgtcgc tcgccgctct gctgccgccg cgcgcgcacg ccgccgctgc
     1441 cgggccgctg ctcgaggtcg cctcgccgga ggccaaggcg gtcaagtacg tcgaagatgc
     1501 cgccacggcg aacggcgcca ctgccgggag cacctgcgcc aattgcgcgc tctaccaggg
     1561 cgccgacggc tccaagcagg gcccatgcca gatcttcccc ggcaaggagg tcaaggccgc
     1621 cggctggtgc acctcatggg cgccgcagat gtgaggggga cgccgcaagg agagtgcgcg
     1681 cgagcgacat tggggcaggg taaggcattc cgctgcgccc ccagactcaa ggagcgagcg
     1741 ccatgtcgag tttcatgctg ctgcttcatc gtccgctcaa tggcgcggag cgcgagtacc
     1801 tgacgatgaa gctgcagcgc tgcctcgccc ggggcgcttc ggcgtactat tcccggctcg
     1861 caaggagatc gacgttatga accagcccct gctcgaccgg cgtcaattcg ctacgcgctg
     1921 tgcggccttc ggcttggcgt tacccgccat gctggcgacg acggcgcgcg cgcaggctcc
     1981 cggcaccgct cccgcggcga acacggcgaa gcccgccgag cgtaccgtga agctgccgga
     2041 cggcactgcc gtaactgcgc tcggtcaggg ctgctggcat ctcggtcagg gaaggcatcc
     2101 ggcggcggta gaggaagagg cgctgcgcac cggcatctcg cttggcatga cgttgctcga
     2161 cacctccggg aattacggac acggccgctc cgaggagttc ctccgccacg tcctggccgg
     2221 cgagcgcgag cgtatcttcc tcgtctccaa ggtcgagggc gatgaaatct ccggcgacgg
     2281 catcgcgcgt gcctgcgcgg cgagcctcgc gcggctcggc accgactatc tcgatctgta
     2341 tctgctgcac tggccggtcg ccagcgcgca attcgccggc gtagtcgccg gattcgagcg
     2401 actacgtgct gcccggaaga ttcgcgcctg gggcgtatcc aacttcaacg tcggccagat
     2461 ggaggatctg ctgcgcgttc cggacggcca tcgctgcgcg accaatcagg tcccttacag
     2521 cctcaacaac cgtcatatcg agagtgacgt cctgccctgg tcgaggcagc acaatatgcc
     2581 ggtgatggcc tactcaccgc tcggcggcga caatcatctg gtggtgggtg agcgcacgct
     2641 ggcgcagatc ggcgcggctc atggctgctc ggccgccgcg gtcgcgctcg cctgggcgat
     2701 tcgtggcggc aacgtcatcg caattcccga gtccggctcg ccggcgcacg tgcgggaaaa
     2761 cgccgcggcg ctctcgatcg cgctcacgcc gcaagacctg cagacgctga atgcagcgta
     2821 tccggggcca gccggcacaa gctagcgcgc tcactcgtag cagggaccga tctcgcgcac
     2881 ctcgatcgta gcccagccgg ccgcggggca ctcgccggca aaggccaggg ccgcctcaag
     2941 gctgctcacg ttgagaagga agaatccccc gaccagctcc ttggcctcgg tgaacggtcc
     3001 gtcgactacg gtgcgcttgc cgccgcgaac cgtgagccgc gcgccggcgg acttcagcga
     3061 gttggtggcg aggaggacgc cgcgtgcctt gaggctctgc gcgtactcga gcatttggtc
     3121 gtacgcaacc tggccggcct cgcgtccccg gtcatgcctc tgcccggtcg gctcgacaat
     3181 caacagcatg taggacatgg tctctccgtt cagctgcgca cacacgcggc ggcgcgatcg
     3241 aggagcaggc ggctctcgcg ggcattgcgc gtcagctgcg cggcgcgctc gaactccccg
     3301 cgtgcctcgg cgtagcggcc gagcttcgcg agcaggtcgc cgcgcacgct cggcagcagg
     3361 tgataggact tgagtgtgcc ttcggccgcg agccgatcgg cgagctcgag tccggcggcc
     3421 ggcccgtagg ccatcgatac tgccactgcg cggttgagct cgacaaccgg cgtcggcgtg
     3481 agccgcacga gctcatcgta aagcgcggtg atgtagaccc agtccgtgtc cgccgcggtc
     3541 gcggcacgtg cgtggcaggc ggcaatcgcc gcctgcagca gatatggccc gcgggtctcc
     3601 ccgagcacct cgccgcgccc gagcgccatg aggccccggt gcagcagcac gcggtcccag
     3661 agcgcgcggt tctgctcgag gagcagcacc ggctcgccgc gggcgttggt gcgcgcgcgg
     3721 atccgcgagg cctggatctc catgagcgct accaggccgt ggacctcgct ctccccgggc
     3781 gcaagctcgg cgagaatgcg cccgatgcgc agggcgtcct cgcacagcgc gggccgcatc
     3841 cagtcagagc cggcggtcgc cgcatagccc tcgttgaaga cgagatagat gacctcgagg
     3901 accgaggaaa gccgccgccc cagctcctcg ccacgcggaa tctcgaacgg tacgcccgcc
     3961 tcgctcagcg tgcgcttggc gcgcacgatg cgctgcgcga tggtgggctc gggcacgagg
     4021 aaggcgcggg cgatctcggc ggtggtgaga ccgccgagca gccgcagcgt gagcgcggtg
     4081 cgcccctcgg tgggcagcac aggatgacag gcggtaaaga tgagccgcag cagatcgtcg
     4141 ccgatctcat cgtcggccgc cgtctcgacg ccgtcgcggt gctgctggtc catttcgagc
     4201 tcggcgctaa gggcttcgtg cttgcccgca cgcatgcgct ggtggcgcag ccggtcgatg
     4261 gcgcggttgc gtgcggtcgc catgagccag gcacccgggt tgtcggggat gccgctgccg
     4321 ggcgactgct cgagcgccgt gacgagcgca tcctgcgcca gctcctcggc cacgcccaca
     4381 tcgcgcgtca tgcgcgcgag cgcgccgatc agcttcgccg actcgatcct ccagaccgca
     4441 tcgatggtgc gctggacttc cgtggccttc atggccaggg atgacatcac cgccagctgc
     4501 accgcgcaag cgaggatcac tcaggcactt ttccgcgcgg ccgcggcgag ggcttccggg
     4561 ggcttggcgc agttgaccat ccagggcgta ccgaagcggt ccgtgcacat gccgaagcgc
     4621 caggcccaga aggtttcctg gatcggcatg tcgaccgtgc cgccctcgga gagggtgcgg
     4681 aagagccgct cggcatcacc ggggctcgtg gcgttggcca tgacgttgaa gccctgcggc
     4741 ttctcatagc gatcgggcgg tacgtcgcag ccaaggagcg cgtcgtcacc gagatcgacc
     4801 cgcgcgtgga tgatctggtc gcgcacttcc ggcgctgccg gtgcggcgcc cggcgcctcc
     4861 ccgtaggtca tcgaaaagac gatgcggccg ccgaaagtcg cggcgtagaa ccggaaggct
     4921 tcgctgcagt tgccgcggaa atgcaggtgc gtgagggtgg tgggcatggc agtcagttcc
     4981 tcgaaatgcg ttcggcgcgg ctctgctgct gtgcgcgcag ctccggcgtc agggcgggac
     5041 cgaaatcctc cgcctcgaac acgcggcgga tctcgatctg ctcgttcgat ttctggaatg
     5101 gcgagcgccg gatccactcg acggcctcgt cgatcgagcg cacctgccac agccagaatc
     5161 cggcgatcaa ctccctggtc tcggtgaagg ggccgtcgct caccgtgctc ttgccgccgc
     5221 cgaactggac gcgtaccccc ttggagctcg cctgcagccc ttcggcggcc agcagcacgc
     5281 cggccttcac cagctgctca ttgaacttgc ccatcgcagt gagaagctcc tcgctcggca
     5341 gcacgccggc ttctgagtcc cgggtggcct tgacgattac catgactcgc atgcaggtct
     5401 ccttgaatga ggtgtgggtg gcgggcgct
//
LOCUS       DATLKL010000716         3710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_211632, whole genome shotgun sequence.
ACCESSION   DATLKL010000716 DATLKL010000000
VERSION     DATLKL010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211632"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VM713_09660"
     CDS             <1..658
                     /locus_tag="VM713_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVH84569.1"
                     /translation="SSDGVFADPKGFLADAVRHVQAAGGLFIADEVQPGFGRLGETMW
                     GFQRHGVVPDLVTMGKPMGNGHPVAAVAARPDVLARFAKRRHYFNTYGGNSVSCAAAL
                     AVLQVIRDENLLENARRTGEYLLQGLRALADRCPAIAEIRGAGLFLGVELRANPVSGL
                     VGPAETRRVVNGMCRRGVLIGSTGRHGDVLKIRSPLTFEMQHADLLLDTLEQALSARD
                     "
     gene            complement(666..2135)
                     /locus_tag="VM713_09665"
     CDS             complement(666..2135)
                     /locus_tag="VM713_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVH84570.1"
                     /translation="MPLAYGVGHDATTGYTPWIMNEAVTIAPTDTHELSRSLRPRHVA
                     MITIGGIIGAGLFVGSSVAIKAAGPASVISYILTGFLLLLVMRMLGEMAVEMPQVRSF
                     TEFTRVTLGNWAGFSVGWLYWYFWVTVIPVEAIAGAGIIKDWIALPEWVIGTALMVCM
                     TSVNLMSARAYGEFEFWFSSIKVAAIISFIIVVGAHAFGFRSPTGPTFSNLTAYGGFA
                     PLSWFAVLASVTTVIWSMMGAEVVTIAAAESPEPARAVARMTSTIITRILLFYVLSVF
                     VITCVVPWNKVVPAQSPFTLALDEVHFPYASTIMAAVVLTAVLSCLNSCFYIASRVLF
                     VLATRGDAPHWLVRTNRRHVPARAVLLASAFGFAGVIAAILSREGVYVFLTNSSGAII
                     AIIYLMIGVSQVRSRRARERAGLPPSALPMWLFPWLSYFGIAGMASVLVAMAFTPSHR
                     AELWTSTVSVAVALLAYVVLRRPRAVAAPAVESSAAPSR"
     gene            2134..3297
                     /locus_tag="VM713_09670"
     CDS             2134..3297
                     /locus_tag="VM713_09670"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HVH84571.1"
                     /translation="MVGKPLAAVAIFLIAWGAAHGETPAARPRAADLGLRVGVLPPGA
                     LDAITDVAGVAVGHTTIVRGDTVRTGVTAIVPHPGNLFREKVPAAISVGNGFGKLTGV
                     TQVEELGEIETPILLTSTTSVARVADALISYMLQLPGNEDVLSINPVVGETNDGYLND
                     IRGRHITADDVFGALARARGGPVEEGAVGAGTGTVAFGWKGGIGTASRLLSKTLGGYT
                     VGVLVQTNFGGVLTIGGAPVGRELGQYYLHDQAARAGPRRDSADGSCMIVVATDAPLD
                     ARELRRLAARALLGLARTGSSASNGSGDYAIAFSSAPEVRIHTEDKAITRRTEVVTTA
                     AMSPLFEAAIEATEEAIYNSMLKATTTSGNGHTVEALPLERTVQILKEHRLIR"
     gene            complement(3321..>3710)
                     /locus_tag="VM713_09675"
     CDS             complement(3321..>3710)
                     /locus_tag="VM713_09675"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HVH84572.1"
                     /translation="RLRLELGPRAEAGLARYLQVNLWRPIRGPVRDAPLALCDGASVA
                     PQALRAVDLRYPERIGEIYYLMHEPGQRWHFAADMTVDEAWLFKNFDSAPAGPGRAAP
                     HSAFTDPRHPHEAPRESIEVRALAIFR"
BASE COUNT          655 a         1251 c         1282 g          522 t
ORIGIN      
        1 ctcgagcgat ggcgtgttcg cggatccgaa gggattcctc gccgacgccg tgcgccatgt
       61 gcaggcggcc ggggggctct tcattgccga cgaggtgcag ccgggcttcg gccggctggg
      121 cgaaacgatg tggggcttcc agcgccacgg cgtcgttccg gacctcgtca cgatgggcaa
      181 gcccatgggc aacggacacc cggtggccgc cgtcgcggcg cggcccgatg tgctggcgcg
      241 tttcgccaag cgccgccact acttcaacac ctacgggggc aatagtgtct cgtgcgccgc
      301 ggcgcttgcg gtgctgcagg tcatccgcga cgagaatctt ctcgagaacg cgcgccgcac
      361 gggcgagtat ctcctgcagg gtctgcgtgc acttgcggac cgctgccctg cgatcgccga
      421 gattcgcggt gccggcttat tcctcggtgt cgagctgcgc gccaatccgg tctcaggcct
      481 cgtgggcccc gccgagaccc ggcgggtggt caacgggatg tgccgccgcg gcgtgctgat
      541 cggctccacc gggcgccacg gcgatgtgct caagatccgt tcgccgctga ccttcgagat
      601 gcagcatgcg gacctgctgc tcgacacgct cgagcaggcg ctctcggcca gggactgagc
      661 gcgcttcagc ggctcggtgc cgcggaggac tccaccgccg gggccgccac cgcccgcgga
      721 cgccgcaaga ccacgtaggc aagcaatgcc accgcgaccg atacggtgct ggtccacagc
      781 tcggcccgat gcgagggggt aaaggccatc gcgacgagca ccgaggccat tccagcgatg
      841 ccgaagtagc tcaaccacgg gaacagccac atcggcaacg ccgagggagg cagcccggcg
      901 cgctcgcggg cccgccgtga acggacctgg gacacaccga tcatgagata gatgatcgcg
      961 atgatggcgc cggaagagtt cgtgaggaac acgtacaccc cttcccgcga gagaatcgcg
     1021 gcgatcacgc cggcgaagcc gaacgcgctc gcgagcagca ccgcgcgcgc cggcacgtgg
     1081 cggcggttgg tgcgcaccag ccaatgcggc gcatcgcccc gggtcgcgag cacgaacagc
     1141 acccgtgagg cgatatagaa gcaggaattg aggcaggaga gcactgcggt gaggaccacc
     1201 gccgccatga tggtgctggc atacgggaaa tgcacctcat cgagcgcgag cgtgaagggc
     1261 gactgcgccg gcaccacctt gttccacggc acgacacagg tgatgacgaa caccgagagc
     1321 acatagaaaa ggagtatgcg ggtgatgatg gtggaggtca tgcgggctac cgcgcgcgcg
     1381 ggctccggcg actccgccgc ggcgatcgtc accacctccg ctcccatcat ggaccagatg
     1441 accgtggtca cggacgcgag caccgcgaac cagctcagcg gcgcgaaccc gccgtaagcg
     1501 gtgaggttcg agaaggtcgg ccccgtcggc gaacggaagc cgaacgcgtg ggcgccgacc
     1561 acgatgatga aggagatgat ggccgcgacc ttgatggagg agaaccagaa ctcgaactcg
     1621 ccgtaagcgc gcgccgacat cagattcacg gaggtcatgc acaccatgag cgcggtaccg
     1681 atcacccact ccggcagcgc tatccagtcc ttgatgatcc cggcaccggc gattgcctcg
     1741 accggtatca ccgtcaccca gaaataccag tagagccagc cgaccgagaa gccggcccag
     1801 ttgccgagcg tcacgcgcgt gaactcggtg aaggagcgca cctgcggcat ctcgaccgcc
     1861 atctcgccca gcatgcgcat gacgagcagc agcaggaagc cggtcaggat gtagctgatg
     1921 acgctcgcgg gcccggcagc cttgatggcc acgctgctgc cgacgaagag cccggcgccg
     1981 atgatgccgc cgatggtgat catcgcgaca tgccgggggc ggagcgagcg gctgagctcg
     2041 tgggtgtcgg tcggagcgat cgtgactgct tcgttcatga tccagggagt atagcccgtc
     2101 gtggcatcgt gcccgacacc gtatgctaac ggcatggtcg ggaagcccct tgccgcggtc
     2161 gcgatcttcc ttattgcctg gggcgccgct catggcgaga cgccggccgc gcgcccgcgc
     2221 gccgccgacc tgggactgag ggtgggagtc ctgccgccgg gtgcgctcga cgcgatcacc
     2281 gatgtggcgg gcgtggcggt cggacacacg accatcgtcc gcggcgacac cgtccgtacc
     2341 ggcgtgacgg cgatcgtgcc gcatcccggc aacctcttca gggagaaggt gccggccgcc
     2401 atctccgtcg gcaatggctt cggcaagctg acaggcgtga ctcaggtcga agagctcggc
     2461 gagatcgaga cgccgatcct cctcacctcc acgacgagcg tagcgcgcgt cgcggacgcc
     2521 ctcatcagct acatgctgca gcttcccggc aacgaggacg tgctgtcgat caatcccgtg
     2581 gtcggcgaga ccaacgacgg gtacctcaac gacatccgcg gtcgccacat cacggcggac
     2641 gacgtgttcg gcgcgctcgc gcgcgcccgc ggtggcccgg tcgaagaagg ggccgtgggc
     2701 gccggaacgg gaacagtggc cttcggctgg aagggcggca tcgggaccgc gtcgcgcctg
     2761 ttatcgaaga cgctcggcgg ctatacggtc ggcgtcctgg tgcagaccaa tttcggcggc
     2821 gtgctgacga tcgggggagc tcccgtggga cgcgagctcg gccagtacta tctgcacgac
     2881 caggccgcgc gcgccggccc ccgccgcgac agtgccgacg gatcgtgcat gatcgtcgtg
     2941 gcgacggatg caccgctcga cgcgcgcgag ctgcggcgcc tcgcggcccg cgcgctgctc
     3001 ggactggcgc gaaccggcag ctcggccagc aacgggagcg gtgactacgc gatcgccttc
     3061 tcgagcgccc ccgaggtacg cattcacacc gaggacaagg cgattacccg caggacggag
     3121 gtggtcacca cggcggcgat gtcaccgctc ttcgaggcgg ccatcgaagc gaccgaggag
     3181 gccatctaca actccatgct caaggcgacg accacgagtg gcaacggaca caccgtcgag
     3241 gcgctgccgc tcgagcgcac ggtgcagatt ctcaaagagc atcggctgat ccgctgaaga
     3301 gcgcgactcc tgcgcacgcc tcagcggaag atcgccagcg cgcggacctc gatgctctcg
     3361 cgcggcgcct cgtgcggatg gcgcggatcc gtgaacgcgc tgtgcggtgc agcgcggccc
     3421 ggtccggcgg gcgccgagtc gaaattcttg aacagccatg cctcatcgac cgtcatgtcc
     3481 gcggcgaaat gccagcgctg ccccggctcg tgcatgaggt agtaaatctc gccgatgcgc
     3541 tccggatagc gcaggtcgac ggcgcgcagt gcctgcggcg cgacgctcgc cccgtcacat
     3601 agcgccagcg gtgcatcgcg caccggaccg cggatcggtc gccacaggtt gacctgcagg
     3661 taccgcgcca gtcccgcctc ggcgcgcggg ccgagctcca gacgcagacg
//
LOCUS       DATLKL010000717         1542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_110123, whole genome shotgun sequence.
ACCESSION   DATLKL010000717 DATLKL010000000
VERSION     DATLKL010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110123"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VM713_09680"
     CDS             <1..804
                     /locus_tag="VM713_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HVH84573.1"
                     /translation="DPALRSSAALVLDLSNSSVLYSRRSDVALPIASITKLMTALVVM
                     DTGQPLDEIVEISAEDVAHRRGAVSRLTPGVQLTRGDLMHLALMASENRVAHALGRTY
                     PGGLEACVAAMNAKARELGMEATRFVEPTGLSDENVASAEDLSKLVIAAAKVPAIREY
                     STDTSYEVRVGRRLLTYRSTDSLTSKPDWDIVVQKTGYIHSAGRCLVMQTVIDARTVV
                     IVLLNSYGKRSRVADARRIRRWMESSERVHGATAPVSPSESVAPAPAAT"
     gene            complement(825..>1542)
                     /gene="ftsX"
                     /locus_tag="VM713_09685"
     CDS             complement(825..>1542)
                     /gene="ftsX"
                     /locus_tag="VM713_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HVH84574.1"
                     /translation="KADVALAKAAQLAAAARARADVAEVTLIPADQGLADFRSYSGFG
                     DALAALKENPLPHVLHVRPRAEASSPAALERLHGYFTAWPEVELVQVDSQWVQRFNAI
                     LEVLRQLLLMAAALLGVGVLAVIGNTIRLEIAGRRVEIEVIKLVGGSNSFVRRPFLYT
                     GLLYGLGGALLAWGIIASGVGLLGEPVDNLARLYGSHFQLRGPSPAEIGMLLGAGAGL
                     GWLGAWISAARHLRSIEPRA"
BASE COUNT          253 a          550 c          514 g          225 t
ORIGIN      
        1 gatccggcgc tgcgctcgag tgcggcactg gtactcgatc tcagcaactc ctcggtgctg
       61 tactcgcgcc gctcggatgt tgccctgccg atcgcctcga tcaccaagct catgacggcg
      121 ctcgtggtaa tggataccgg ccagccgctc gacgagattg tcgagatcag cgccgaggac
      181 gtggcgcacc gcaggggcgc cgtctcacgg ctgactccgg gcgtgcagct gacgcgcggc
      241 gatctcatgc acctggcgct catggcgtcc gagaaccgcg tggcgcatgc gctcggacgc
      301 acttaccccg gcggcctcga ggcatgcgtg gccgcgatga atgccaaggc acgggagctc
      361 ggcatggaag ctacgcgctt cgtcgagccg accgggctct cggatgagaa tgtcgcgagc
      421 gccgaggatc tctcgaagct ggtgatcgcg gcggcgaaag tcccggccat ccgcgaatac
      481 tccaccgaca cgagctatga ggtgcgcgtc gggcgccgcc tgctcaccta ccgcagcacc
      541 gattcgctca cctcgaagcc cgactgggat atcgtggtgc agaagaccgg ctacattcac
      601 tcggccggcc gctgcctcgt catgcagacg gtcatcgatg cgcgcaccgt ggtgatcgtg
      661 ctgctcaact cctacggcaa gcgcagccgc gtcgccgacg cgcgccgtat ccgccgctgg
      721 atggaaagct ccgagcgcgt gcacggagcg acagcgcccg tctcaccgtc cgagtccgtc
      781 gcgcccgcac ccgcggcgac ctgagtcgat cacgctccgc tgcctcaggc gcgcggctcg
      841 atgctgcgca gatgccgtgc ggccgagatc cacgccccga gccagccgag ccccgcaccg
      901 gccccgagca gcatgccgat ctcggcgggc gacggaccgc gcagctggaa gtggctgccg
      961 taaagccggg ccaggttgtc gaccggctcc ccgagcagcc ctactcctga tgcgatgatt
     1021 ccccacgcga gcagcgcgcc gcccaggccg tacagcaatc cggtgtagag gaaggggcgg
     1081 cgcacgaagg aattcgagcc gccgaccagc ttgatgacct cgatctcgac acgccgcccg
     1141 gcgatctcga ggcggatggt gttgccgatc acggcgagca ccccgacgcc gagcagtgcc
     1201 gccgccatca gcagcagctg ccgcagcacc tcgaggatcg cgttgaatcg ctgcacccac
     1261 tgcgaatcca cctgcaccag ctcgacttcc ggccaggcgg tgaaatagcc gtgcagccgc
     1321 tcgagcgccg ccggcgaact cgcttcggcg cgcggccgca cgtgcagcac gtgcggcagg
     1381 ggattttcct tgagcgcggc gagtgcgtca ccgaatcccg agtagctgcg gaagtcggcg
     1441 agaccctggt cggcgggaat cagcgtcacc tcggcgacat cggcacgcgc gcgtgccgcg
     1501 gcggcgagct gcgcggcctt ggcgagcgcc acgtcggcct tg
//
LOCUS       DATLKL010000718         1625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_189259, whole genome shotgun sequence.
ACCESSION   DATLKL010000718 DATLKL010000000
VERSION     DATLKL010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189259"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VM713_09690"
     CDS             <1..969
                     /locus_tag="VM713_09690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HVH84575.1"
                     /translation="YRVEAYAAEPTPQNAINEKAIVDAEAVGEAIRRAVKRAGAKSNE
                     VAVAISGDAAITKVIQMPRALRSGDLEAQVEMQADQYIPFPMDEVSYDLEVLGPNDKD
                     PESNDVLLVATRTENVEQRQAAVKSAGLAARIVDVEAFALENACKLMTHQMPDGGIDR
                     TIAVVDFGASSTTFSVLRNLKVVYTRDIAFGGQQLTEEIMRTYGLSMEEAGRAKKEGG
                     LPGNYQSEVLDPFIDDMTQQVSRSLQFYLASGSGREQPEKIVVCGGCANIPGVAEVIS
                     SRVGIAAEKGDPLGQMRLSSRAKAQAVQRDATALLTACGLALRSFD"
     gene            971..1603
                     /locus_tag="VM713_09695"
     CDS             971..1603
                     /locus_tag="VM713_09695"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HVH84576.1"
                     /translation="MPRINLLPWREAQRKERKLAFLVALGVAVLAALVTTFAVYLMYD
                     SMIAGRQRRNQLLRTQIKQLDKQIEEINSLETEKQRFIARMDVIEKLQRSRPGIVHVF
                     DEIVKTLPDGVYLTGVQQVSAAGANKRLKFEGVAQSSTRVSSFMRNIDTSEWLRNPEL
                     EVVQTAKGPGSNFTLTADEVTAGGQEEPGKNPVGKGKIRKADLGGAGVQQ"
BASE COUNT          316 a          512 c          553 g          244 t
ORIGIN      
        1 taccgcgtcg aggcctacgc ggcagagccc acgccacaga acgccatcaa cgagaaagcc
       61 atcgtcgatg ccgaggcagt gggcgaggcg atccggcgcg ccgtgaagcg cgcgggcgcc
      121 aagagcaacg aggtggcggt cgccatctcc ggcgatgctg caatcaccaa ggtgatccag
      181 atgccgcgtg cgctgcgctc gggtgatctc gaggcacagg tcgaaatgca ggcggatcag
      241 tacatcccct ttccgatgga tgaggtgagc tacgacctcg aggtgctcgg accgaacgac
      301 aaggatcccg agagcaacga tgtgctgctg gtggcgacgc gtaccgagaa cgtcgagcag
      361 cgtcaggcgg cagtgaagtc cgcggggctc gcggcacgca tcgtcgacgt cgaggccttc
      421 gcgctcgaga atgcctgcaa gctcatgacg catcagatgc ccgacggcgg tatcgatcgc
      481 accatcgcgg tggtcgactt cggtgcgagc agcaccacct tcagcgtgct gcgcaacctc
      541 aaagtggtgt atacgcgtga catcgccttc ggcgggcagc agctcaccga ggagatcatg
      601 cgcacctacg ggctgtcgat ggaggaggcg ggccgcgcca agaaagaagg tggcctgccc
      661 ggcaactacc agtcggaagt gctcgatccc ttcatcgacg acatgacgca gcaggtgagc
      721 cgctcgctgc agttctatct ggcctcgggc tccgggcgtg agcagccgga gaagatcgtg
      781 gtgtgcggcg gctgcgccaa catcccgggt gtcgccgagg taatttcgag ccgtgtgggc
      841 atcgccgccg agaagggcga cccgctcggc cagatgcgcc tgtcctcgcg cgccaaggcg
      901 caggcggtgc aacgcgacgc cacggcgctc ctgactgcgt gcgggcttgc actgcggagc
      961 ttcgactgac atgccacgca taaatctact tccctggcgc gaggcgcaac gcaaggagcg
     1021 caagctcgcc ttcctcgtgg cgctcggggt cgcggtgctc gccgccttgg tgaccacctt
     1081 cgccgtctac ctcatgtacg actcgatgat cgccgggcgg cagcgccgca atcagttgct
     1141 gcgcacccag atcaagcagc tcgacaagca gatcgaggag atcaacagcc tcgagaccga
     1201 gaagcagcgc ttcatcgcgc gcatggacgt catcgagaag ctgcagcgct cgcggcccgg
     1261 gatcgtgcat gtgttcgacg agatcgtgaa gacgctcccc gacggggtgt atctcaccgg
     1321 tgtgcagcag gtcagcgccg ccggcgccaa caagcgcctg aaattcgagg gcgtggcgca
     1381 gtcgagtacg cgcgtctcct ccttcatgcg caacatcgac acctccgagt ggctgcgcaa
     1441 ccccgagctc gaggtggtgc agaccgccaa ggggccgggc tcgaacttca cgttgacggc
     1501 cgacgaggtg accgcgggag gccaggaaga gccgggcaag aatccggtgg gcaagggcaa
     1561 gatccgcaag gcggacctcg gcggcgcggg agtgcagcaa tgaacgtcct cgaggaactg
     1621 cggtc
//
LOCUS       DATLKL010000719         1659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_200329, whole genome shotgun sequence.
ACCESSION   DATLKL010000719 DATLKL010000000
VERSION     DATLKL010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200329"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VM713_09700"
     CDS             complement(<1..646)
                     /locus_tag="VM713_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HVH84577.1"
                     /translation="MRGSILATGKRIDGRDTKSIRPIVCEVGVLPRAHGSALFTRGET
                     QALVTTTLGTGQDEQIIDALEGEYREHFLLHYNFPPYSVGEAGFLRAPGRREVGHGKL
                     AWRAVHPLMPAKDRFPYTIRVVSEITESNGSSSMATVCGASLSLMDAGVPLARPVAGI
                     AMGLIKETSGLAVLSDILGDEDHLRDMDFKMAGTERGVTSLQMDIKITSITEAIM"
     gene            complement(665..1555)
                     /locus_tag="VM713_09705"
     CDS             complement(665..1555)
                     /locus_tag="VM713_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84578.1"
                     /translation="MFQIYRKELVWGGRTLTLETGRIARQADGAILATYGETTVLATA
                     VAQKTVKPGVDFFPLTVNYQEKTFAAGKIPGGFFKREGRPSEKETLVSRLIDRPIRPL
                     FAPGFTNETQIICTTLSHDLDNDPDIAAMVAASAALTISGIPFMGPIAACRVGRINGE
                     LVLNPKMDEMANSDLDLVVAGTAEGVLMVESEAKELPEEVMLAAVMFGHQQYQPVIRA
                     IIELAEACAKDPWQLPGSPADKERAEARLRELAEAPLRAAYGETLKQVRVERVAAAKT
                     EALTRMIEEGFDAELVGSLL"
BASE COUNT          268 a          553 c          534 g          304 t
ORIGIN      
        1 tcatgatcgc ttcggttatg ctggtgatct tgatgtccat ctggagcgag gtgacgccac
       61 gctcggtgcc agccatcttg aagtccatgt cgcggagatg gtcctcgtcg cccaagatgt
      121 cggacagcac ggcgaggcca ctcgtttcct tgatcagacc catggcgatg ccggccactg
      181 ggcgcgcgag gggaacgccc gcatccatca gggaaagcga cgcgccgcac acggtcgcca
      241 tcgaagagga gccgttcgac tcggtgatct cggagaccac gcgaatcgtg taggggaaac
      301 gatccttggc gggcatcagc ggatgaacgg cgcgccaagc gagcttgcca tggccgacct
      361 ctcgtcggcc tggtgcccgc aggaagcccg cctcccccac ggagtagggc gggaagttgt
      421 agtgcaacag gaaatgctcg cggtactcgc cctcgagggc gtcgatgatc tgctcgtcct
      481 ggccggtgcc gagcgtggtg gtgacgagcg cctgggtctc gccgcgggtg aagagcgcgc
      541 tgccatgcgc ccgcggtagc acccccacct cgcagacgat gggccggatg ctcttagtgt
      601 cgcggccgtc gatgcgtttg cccgtcgcga gaatgctgcc gcgcacgatg tccttctcga
      661 gcggctagag caatgagccg acgagctcgg catcgaagcc ctcctcgatc atcctggtga
      721 gcgcctcggt cttggccgcg gccacccgct cgacgcgcac ctgcttgagc gtctcgccgt
      781 aggctgcgcg caacggcgct tcggcgagtt ccctcaaccg cgcctcggcg cgctccttgt
      841 cggcgggcga ccctggcagc tgccagggat ccttggcgca agcctcggcc agctcaatga
      901 tcgcccggat gaccggctga tactgctggt gtccgaacat gacggccgcg agcatgacct
      961 cctcgggcag ctccttggct tccgattcga ccatgaggac gccctcggcc gtgcctgcaa
     1021 ctaccaggtc gagatccgag ttcgccatct cgtccatctt cggattgagc accagctcgc
     1081 cgttgatccg cccgacgcgg cacgccgcga tcggccccat gaacggaata cccgaaatcg
     1141 tgagcgccgc cgacgcagcc accatcgccg cgatgtcggg gtcgttatcg aggtcgtggc
     1201 ttagagtggt gcagatgatc tgggtttcgt tcgtgaagcc cggcgcgaag agcggccgga
     1261 tgggccggtc gatcagtcgc gagaccagcg tctccttctc gctcgggcgg ccctcgcgct
     1321 tgaagaagcc cccggggatc ttgcccgccg cgaaggtctt ctcctggtaa ttgaccgtga
     1381 gggggaagaa atcgactcca ggcttgaccg tcttctgcgc cacggcggtg gcgagcaccg
     1441 ttgtctcgcc gtaggtcgcc aggatggccc cgtccgcctg gcgggcgatc ctgccggtct
     1501 caagcgtcag cgtgcgtccg ccccaaacaa gttccttccg atatatctga aacatctctc
     1561 tcgttccttc cgcattccgg cccgtgccgg accaaaaagt tgccatcacc tgcgggcgcg
     1621 catccgtaga aattcggacc gggacgcgcg cccgctgtg
//
LOCUS       DATLKL010000720         2290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_76588, whole genome shotgun sequence.
ACCESSION   DATLKL010000720 DATLKL010000000
VERSION     DATLKL010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76588"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VM713_09710"
     CDS             complement(<1..384)
                     /locus_tag="VM713_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HVH84579.1"
                     /translation="MSLGPLMIDLGGSALTAEERELLRHPLVGGVLLFTRNYVEPEQL
                     AALVAEIHQARAPALIVAVDHEGGRVQRFRAGFSLLPPARRIGHEFDAEPRAGLALAR
                     QLGWLMAAELRARGVDISFAPCVDLD"
     gene            complement(381..1451)
                     /gene="rlmD"
                     /locus_tag="VM713_09715"
     CDS             complement(381..1451)
                     /gene="rlmD"
                     /locus_tag="VM713_09715"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HVH84580.1"
                     /translation="MQHFAPAAQLSAKEAELKSTLERVARVTPRRWLEPLTGPVWAYR
                     RRARLGAKFVQRKGRVVVGFRERAAPYVADLSGCEVLAPPTGALIAPLAALLTGLGIR
                     EHVPQIEVAVADNATALVLRVLAEPSQADLAQLEQFAHRHGVRLYLQPAGLDSVRALG
                     AQPAEPLRYHLRAFGLELEFSPTDFIQVNGAVNEALVARAAELLELNPAARVLDLYCG
                     LGNFTLALARRAASVVGVGGEPQLVARARANGARNTLANAEFHQADLAAAPSAQAAWL
                     GRSYSHVVLDPPRTGARALLATVAGLAPERVLYISCHPGSLARDLMALVHEHGFRLEA
                     AGVVDMFAHTAHVESLALLSRP"
     gene            complement(1464..1706)
                     /locus_tag="VM713_09720"
     CDS             complement(1464..1706)
                     /locus_tag="VM713_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84581.1"
                     /translation="MNAAAPGLPVEEIALVGGLTHEGEGVVRGGKTVFVPGALPGERI
                     RLRRTRRHRRHDDGELLEVLTPSPARVAPRCVHFGV"
     gene            complement(1703..>2290)
                     /locus_tag="VM713_09725"
     CDS             complement(1703..>2290)
                     /locus_tag="VM713_09725"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HVH84582.1"
                     /translation="KADSFTLWSVGEVAASEAELVQRFFDGIAKFTPDLVSWNGSGFD
                     LPVLSYRALLWGVQAPRFWETGEGETEFRYNNYLQRYHWRHTDLMDVLSGFSSRNRVS
                     LANMACLLKLPGKLGFAGDQVWNAYQRGDIVGIRRYCETDVLNTYLIYLRFEQLRGRL
                     TPAGYREEVERVRAHLRASSEPHLAQYLAAWTEVV"
BASE COUNT          359 a          800 c          829 g          302 t
ORIGIN      
        1 atcaaggtcc acgcaggggg cgaacgagat gtccaccccg cgagcgcgca gctcggcggc
       61 catcagccag ccgagctggc gcgccagcgc gagcccggcc cgcggctctg catcaaactc
      121 gtggccgatg cgccgcgccg gcggcagcag cgagaagccg gcgcggaaac gctgcacccg
      181 tccgccttcg tggtcgaccg cgacgatgag cgccggcgcg cgcgcctggt gtatctcggc
      241 gacgagcgcg gcaagctgct ccggctcgac gtagttgcgg gtgaagagca gcacgccgcc
      301 caccagcggg tgccgcagca gctcgcgctc ctcggccgtg agggcgctcc ctccgagatc
      361 gatcatcaga ggtccgaggc tcatgggcgg gagagcagcg ccagtgactc gacgtgcgcg
      421 gtgtgcgcaa acatgtcgac gacgcccgcc gcctcgagcc tgaagccgtg ttcgtgcact
      481 agcgccatga ggtctcgcgc gaggctcccc ggatggcacg aaatatacag cacgcgctcg
      541 ggcgccagcc cggccaccgt ggcgagcagc gcccgcgccc cggtacgcgg cggatcgagg
      601 actacgtggc tgtagctccg gccgagccat gcggcctgcg cagagggcgc cgctgccagg
      661 tccgcctggt gaaactcggc gttggcgagg gtgttacgtg cgccattggc tcgcgcgcgc
      721 gccaccagct gtggctcgcc cccgacgccc accacgctcg ctgcgcggcg cgccagcgcg
      781 agcgtgaagt tgccgagccc gcaataaaga tcgagcacgc gcgcggcggg gttgagctcg
      841 aggagctcgg ccgcgcgtgc caccagcgcc tcgttcacgg cgccattgac ctggatgaag
      901 tcggtcggag agaactccag ctcgaggccg aaggcgcgca ggtggtagcg cagcggctcg
      961 gccggctgcg cgccgagcgc gcgtaccgaa tcgagccccg ccggctgcag gtagaggcgc
     1021 acgccgtggc ggtgagcgaa ttgctcgagc tgcgcgaggt ccgcctggga aggctcggcg
     1081 aggacccgca gcacgagcgc ggtcgcgttg tcggcgaccg cgacttcgat ctgcggcacg
     1141 tgctcgcgga tcccgaggcc cgtgaggagc gctgcgagcg gcgcgatcag tgcgcccgtg
     1201 ggcggcgcca gcacctcgca gccactgagg tcggccacgt agggggcggc gcgctcgcgg
     1261 aagcccacca ccacgcgacc cttgcgctgg acaaattttg cgccgaggcg tgcacggcgc
     1321 cggtaggccc agaccggccc ggtgagaggc tcgagccagc gccggggtgt tacgcgcgcg
     1381 acgcgttcca gcgtcgactt gagctccgcc tccttggcag agagttgcgc cgcgggcgca
     1441 aagtgctgca gggcgcagcc ccctcagacc ccgaaatgca cacagcgcgg cgccacccgc
     1501 gccggcgagg gcgtgagcac ctcgagcagc tcgccgtcgt cgtgccggcg atggcgccgc
     1561 gtgcggcgga ggcgaatccg ctcgccgggg agcgctcccg ggacaaacac cgtcttgccg
     1621 ccgcgcacga cgccctcccc ctcatgggtg agtccgccga cgagggcgat ctcctcgacg
     1681 ggcaggccgg gagcggctgc gttcacacga cctcggtcca cgcggcgaga tactgcgcaa
     1741 gatgtggctc gctcgaggcg cgcaggtgcg cgcgcacgcg ctcgacctcc tcgcggtagc
     1801 ctgccggcgt cagccgcccg cgcagctgct cgaagcgcaa gtagatgaga taggtattga
     1861 gcacgtcggt ctcgcagtaa cggcggatgc cgacgatgtc gccgcgctgg taggcgttcc
     1921 agacctggtc gcccgcgaag ccgagcttgc ccgggagctt gaggaggcac gccatgttgg
     1981 cgagcgacac gcggttgcgc gagctgaagc ccgacagcac gtccatgaga tcggtgtgcc
     2041 gccagtgata gcgctgcagg tagttgttgt agcggaactc ggtctcgccc tcgcccgtct
     2101 cccagaagcg cggcgcctgc accccccaga ggagcgcgcg gtaactcaac accgggagat
     2161 cgaagcctga gccgttccag gacacgaggt cgggggtgaa tttagcgatg ccgtcgaaga
     2221 agcgctgcac cagctcggct tcgctggccg ccacctcgcc gacgctccat agcgtgaagc
     2281 tgtcggcctt
//
LOCUS       DATLKL010000721         1823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_222599, whole genome shotgun sequence.
ACCESSION   DATLKL010000721 DATLKL010000000
VERSION     DATLKL010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222599"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VM713_09730"
     CDS             <1..689
                     /locus_tag="VM713_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HVH84583.1"
                     /translation="DGAPGIDIAWNTNLAFLQADAWSAHRCRPIAMRDTDLGWRTKIV
                     AVSGGRVTGLDELRNSTLALGSRDSGHAAILPVHFLEAEELFEGRDYRALRFNSDLGK
                     HGDTGASEVDVVRAVLDGRAEAGAIGSPFWNTVKTQRLVPEGALTEVWTSPPYNHCMF
                     TVRPDLDQAAEQRFAAALFGMSYDNPKHRAVLEAEGLRRWVAPELEGYQVLRSAAARQ
                     GFFERATAEG"
     gene            complement(691..1719)
                     /locus_tag="VM713_09735"
     CDS             complement(691..1719)
                     /locus_tag="VM713_09735"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVH84584.1"
                     /translation="MKADIDLDIGQMTFASGLVPELIAALRRCQPGELVAVVGDDESL
                     GPELETWCRFTGNSLVEATVAEGRARWVFRRGPVAASAEDNRPVGSRIWLYTNFDCNL
                     RCDYCCVRSSPRAPRRALGLERVRRIASEGAELGVGEFFVTGGEPFLLPDIGAILSAC
                     ASAAPTTVLTNGMLFAGRRLESLRSLPRERVTLQISLDSPTPDRHDHHRGKGTWARAW
                     KGIERARAEGFRVRLAATVSDDAEAEEFAGFLDAQQVPAEDRVIRRIALRGFAPEGLA
                     LARADIVPEITITAEGVYWHPVGADDADLFLTAEIFPLADAFAALRHALERETEHGRR
                     LATIFNCA"
BASE COUNT          335 a          625 c          592 g          271 t
ORIGIN      
        1 aagacggagc gccgggcatc gacatcgcct ggaatacgaa cctcgccttt ctgcaggccg
       61 acgcatggag cgcgcaccgc tgccggccga tcgcgatgcg cgacaccgat ctcggctggc
      121 gcaccaagat cgtggcggtc agtggtggcc gggtaaccgg gctcgacgag ctcagaaaca
      181 gcaccctggc gctcgggagt cgcgacagcg ggcatgccgc gattcttccc gtacactttc
      241 tcgaagctga agaactgttc gaaggacgcg actaccgcgc gctgcgtttc aacagtgatc
      301 tcggcaagca cggcgacacc ggcgcgagcg aagtcgacgt ggtgcgtgcg gtactcgacg
      361 gccgggccga ggctggcgcg atcggcagcc cgttctggaa cacggtgaag acgcagcgcc
      421 tggtaccgga gggcgcattg acagaggtct ggacgtcgcc tccctacaac cactgcatgt
      481 tcacggtgcg acccgatctc gaccaggcgg cggagcagcg cttcgcggcg gcgctgttcg
      541 gcatgagcta cgacaatccg aagcatcgcg cagtgctcga agccgagggt ctgcgccgct
      601 gggtcgcccc cgagctcgaa ggctaccagg tcctgcgcag cgcagccgca cgccagggct
      661 tcttcgagcg cgcgaccgcc gaaggctgag tcacgcgcaa ttgaagatcg tagccaagcg
      721 acgcccgtgc tcggtctcgc gctcgagagc atgacgcagc gccgcaaagg cgtcggcgag
      781 cgggaagatc tccgcggtga gaaacaggtc cgcatcgtct gcgccgaccg ggtgccaata
      841 aacgccctcg gcggtgatcg tgatctccgg aacaatgtcg gcgcgcgcga gcgccagacc
      901 ctccggcgca aaaccgcgta acgcgatgcg gcgaatcacg cgatcctctg ccgggacttg
      961 ctgagcgtcg aggaacccgg cgaactcctc ggcctcagcg tcgtcggaga cggtcgccgc
     1021 caaccgcacc cggaagccct cggcacgggc tcgctcgata cccttccacg cgcgcgccca
     1081 cgttcccttt ccgcggtggt ggtcgtgccg gtcgggagtc gggctgtcca ggctgatctg
     1141 cagcgttact cgttcacgcg gcaagctgcg caggctctcg aggcgccgcc ccgcgaacag
     1201 catgccgttc gtgagcacgg tagtcggcgc cgcgctggcg caggccgaga ggatcgcgcc
     1261 gatgtccggc aagagaaagg gctccccccc ggtcacgaag aactcgccga caccgagctc
     1321 ggctccttcg ctcgcaatcc gccgcacgcg ttcgaggccg agcgcgcgcc gcggtgcccg
     1381 gggtgaagag cgcacacaac agtagtcgca gcgcaggttg cagtcgaaat tcgtgtacag
     1441 ccagatccgc gaccccaccg gacggttatc ctcggcggaa gccgcaacag gcccacgacg
     1501 gaagacccac cgcgcacggc cttccgccac cgtagcctct accagagaat taccggtgaa
     1561 gcggcaccaa gtctcgagct cggggccgag gctctcgtca tcgcccacca cggcaacaag
     1621 ttcgcccggc tggcagcggc gcagcgcggc gatcagctcc ggcacgagcc cggacgcaaa
     1681 ggtcatttgc ccaatgtcga ggtcgatatc agccttcatt gcaggcggct cgcgccgagg
     1741 tttgccgttc ctcagtgcgc ctcgatccag tccttcgagc ccttgagctt gaccgcgaca
     1801 ttcagaatga caatccgaaa ttt
//
LOCUS       DATLKL010000722         1627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_177849, whole genome shotgun sequence.
ACCESSION   DATLKL010000722 DATLKL010000000
VERSION     DATLKL010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177849"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VM713_09740"
     CDS             complement(<1..292)
                     /locus_tag="VM713_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84585.1"
                     /translation="MTIQGIDRVTYGVADMAAARRFLSDWGLALVDEGAPMLRFETLD
                     GGEVVVRAKDDPALPPAIEPGSTLREVVWGVADATSLTDLGASLRLLPTFDER"
     gene            complement(289..>1627)
                     /locus_tag="VM713_09745"
     CDS             complement(289..>1627)
                     /locus_tag="VM713_09745"
                     /inference="COORDINATES: protein motif:HMM:NF005470.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVH84586.1"
                     /translation="YLDRRRAYIHEARDQLGMLRAVSKAAFRVWSAATALATLKEAVR
                     TALSPPRGPLSVEIPIDVQEAHLPWPADLAPLAPVVQAPSAASLDLLAERLAPACRPL
                     LWLGGGARGAAEAGGRLVAMGFGVVTSTQGRGILPEDHPMSLGAFTASESVEAFYKAC
                     DAMLAVGSRLRGNETLKYALALPRPLYQIDADPRAEGRSYPVALFVCGDARLALEGLA
                     DRLAGRMAIDPAFAGDLAAARERAIAGLSRGLGPYTRLIDALQRAAGRDFVWVRDVTI
                     SNSTWGNRLLRIFGPRDGVHALGGGIGQGLPMGIGAALAANGRRTMCLVGDGGLMLSL
                     GELATAAQERVNLTLLVMNSRGYEVIKNIQDAQFGGRHYYADLLTPDFGQLAASMGWR
                     YHRLADLAAAEAMLGHALGEPGPTMVEVDMDAIGPFVAKFAGPPVRQRAEAKP"
BASE COUNT          253 a          570 c          584 g          220 t
ORIGIN      
        1 cccgctcgtc gaaggtcggg agtaggcgca ggctggcacc gaggtccgtg agcgaggtcg
       61 cgtccgcaac gccccacacc acttctcgca gggtcgagcc cggctcgatc gcagggggca
      121 aggcgggatc gtccttggcg cgcaccacca cctcgccgcc gtccaacgtc tcgaagcgga
      181 gcatcggagc accctcatcc acgagcgcga gcccccaatc gctcaggaac cggcgtgccg
      241 ccgccatgtc cgcgacgcca taggtgacgc gatcgatacc ctggatggtc acggcttcgc
      301 ctcggccctt tggcgtactg gcgggcccgc gaatttcgcg acaaaggggc cgatagcgtc
      361 catgtcgacc tcgaccatcg tcgggcccgg ctcgccaagg gcgtggccga gcatcgcctc
      421 ggccgcggca agatcggcga gccggtggta gcgccagccc atgctggccg cgagctggcc
      481 gaaatcgggt gtcagcaggt cggcataata atgccgcccg ccgaattggg cgtcctggat
      541 gttcttgatg acctcatacc cgcggctgtt catcacaagc agcgtgaggt tgacgcgctc
      601 ctgggcggcg gtcgcaagct cgcccaggct caacatcagt ccgccgtcgc cgaccaggca
      661 catcgtgcgg cggccgttcg cggcgagcgc cgccccgata cccatgggca ggccctggcc
      721 gatgccgccg ccgagcgcgt ggacgccatc gcgcgggccg aagatgcgga ggagccggtt
      781 gccccaggtg ctgttcgaga tggtgacatc gcgcacccac acgaagtcgc gccccgcagc
      841 ccgctgcagc gcatcgatca ggcgcgtgta aggcccgagc ccgcgagaaa ggccggcgat
      901 cgcccgctcg cgcgcggccg ccaggtcgcc tgcgaaggcg gggtcgatcg ccattcggcc
      961 ggcgagccga tcggcgaggc cctcgagcgc cagacgggcg tcgccgcata cgaagagtgc
     1021 gacgggatag gaccggccct cggcccgcgg atcggcgtcg atctggtaaa gcgggcgcgg
     1081 tagcgcgagc gcgtatttga gcgtctcgtt gccgcgcagt cgcgagccca ccgccagcat
     1141 cgcgtcgcat gccttgtaga aggcctcgac gctctcggat gcggtgaagg cgccgaggct
     1201 catcggatgg tcctcgggca gaattccgcg gccctgagtg ctggtgacga cgccgaagcc
     1261 catggcgacg agccggccgc cggcctcggc ggctccccgt gcgccgccgc cgagccacag
     1321 cagcggccgg cacgccggtg caagccgctc ggcgaggagg tcgaggctcg cggcactcgg
     1381 tgcctggacg acgggggcaa gcggcgcgag gtccgccggc caaggcaaat gcgcctcctg
     1441 gacgtcgatc gggatctcga cgcttagcgg gccgcgcggc ggcgagagtg ccgtgcgcac
     1501 cgcctccttg agggtggcga gcgcggttgc tgccgaccag acccggaacg ctgccttcga
     1561 gaccgcgcgc agcatgccga gctgatcacg cgcctcgtgg atgtaggcgc ggcgacggtc
     1621 gagatag
//
LOCUS       DATLKL010000723         4534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_121578, whole genome shotgun sequence.
ACCESSION   DATLKL010000723 DATLKL010000000
VERSION     DATLKL010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121578"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..825)
                     /locus_tag="VM713_09750"
     CDS             complement(91..825)
                     /locus_tag="VM713_09750"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HVH84587.1"
                     /translation="MSFILDALKKSESDRQRQSGPALFEVKVAPPRHALPAWAAAIAV
                     LFVVNLVVVAWLLVRHAAAPAAAPPAQVPAHSEAGTAPAAPAAAPPVSNPPAPAPSQS
                     PSTGTPLPRSVATVPAQAPAQVAAPGLAEARQAAAAAEPTSNPDDFAPAAEPPASGSR
                     GRVKRGTDAGVPLYAQLTATPGTQLPQLRLDLHAYAERAENRWVLINMHKLHEGDSLE
                     GVRVERITPDGAVLSYQGSQFLLTRQ"
     gene            complement(829..2451)
                     /locus_tag="VM713_09755"
     CDS             complement(829..2451)
                     /locus_tag="VM713_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVH84588.1"
                     /translation="MYLKFFGLNEKPFAITPDPRYLYLSERHAEALAHLMYGINEAGG
                     FVQLTGEVGTGKTTIVRSLLAQAPKDAEIALILNPKMTAPEFLLTICEELGIEVPGES
                     RESLKYLVDTLSGHLLEAHAAGRRVVLVVDEAQNLSPEVLEQVRLLTNLETNTQKLLQ
                     IILIGQPELRELLARTELRQLAQRITGRYHLDPLGREETTAYVRHRLRVAGATTDIVS
                     PWALRDVYRLSRGVPRVINVLCDRALLGAYSLDRQRVTGRLVRRAAAEVFGRGFAAYW
                     LPWAAAAVVAAVLAVITASLWALGPWSPGKKPDTAVIAAVPATSAAAAAAAPPVPTLA
                     QLLGAHAAETDTDSAFGKLFALWGAKYQANGTDPCTQAAGAHLECLAARGSFGQLRLY
                     NHPAILMLSDSGGSSHQVVLTGLDDQHARLDLGGAQAVGLGELSHFWLGDFVMLWRPA
                     SSPVKALSAGMRGAEVRWLRQSLERLAHGESSAPVSDVFDAELAGLVRDFQRQHQLSV
                     DGIAGRETQIALASAVAGPDVPLLFTPDTHGG"
     gene            complement(2463..3614)
                     /gene="dinB"
                     /locus_tag="VM713_09760"
     CDS             complement(2463..3614)
                     /gene="dinB"
                     /locus_tag="VM713_09760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HVH84589.1"
                     /translation="MSRAVLHVDMDAFYASVEQHDDPAIKGKPVIVGWTGGRGVVTAA
                     SYEVRPFGVRSAMPMRTALRLCPHAICVRPRMARYQEVSRTVFGVFRDVTPLVEGLSL
                     DEAFLDVTASQELMGGAVEIAHRIKARIRELTGLTASVGVAPNKLVAKIASDLGKPDG
                     LSVVEPERVRAVLDPLSVRRLPGLGRKTGARVEAAGIHTLAELRSAPDAVLWPLFGRY
                     TAWVRERASGVDERPVLPDAEEKSLSAEDTFAEDIGDARALQVQLARLADLACARLRS
                     RELVTACVGVKIRRHDFTTFTRQRAIAPATAAGRVIAGVAAELLERWLESHPEAKLRL
                     LGVVLTELKPASQLGLFEDLRHAQAVDAALDGARARFGARALRRGNTLG"
     gene            complement(3651..4127)
                     /locus_tag="VM713_09765"
     CDS             complement(3651..4127)
                     /locus_tag="VM713_09765"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HVH84590.1"
                     /translation="MMRTFIVAALVLATSLAAVPRAHAQAPAAAPTAAAAMPRTPSPA
                     GAEVYIISPKDGAKVKSPVLVRFGLGDKMGVAPAGVKFDNTGHHHLLIDTDAPANLSA
                     PLPATDNIKHFGKGQTEASITLTPGKHTLQLLLADQNHIPHDPPVISKKITITVEQ"
     gene            complement(4136..>4534)
                     /locus_tag="VM713_09770"
     CDS             complement(4136..>4534)
                     /locus_tag="VM713_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84591.1"
                     /translation="DNGRSVREAFLHNVPRAMFLFLPLLAAGMMLMYWWPRHYYVEHL
                     LLFVHNHAFGFLLLLLAAGVSALLPWAAPWVRFALCLYIPWYVYRSMRMVYGQGRWLT
                     FAKLVALSFFYLISGALMVALTTVYSVLTL"
BASE COUNT          708 a         1519 c         1634 g          673 t
ORIGIN      
        1 cggattctgt gaagctcatt ctgtgacgtg gttcaaggtg tgaagtggtt ccgctccgcc
       61 cggcgcccgg gtccaggcca ctccgacccg tcattgccgc gtcagcagga actgcgaccc
      121 ctggtaggaa aggaccgctc cgtccggagt gatgcgctcg acgcgcacgc cctcgaggga
      181 gtcgccctcg tggagcttgt gcatgttgat gagcacccag cgattctcgg cacgctcggc
      241 gtaggcatgc agatcgaggc gcagctgcgg gagctgtgtg ccgggcgtgg cggtcagttg
      301 cgcgtagagc ggcactcccg catccgtgcc gcgcttcacc cgccctcggc tcccggacgc
      361 aggcggctcg gcggccggcg cgaagtcatc cggattcgag gtcggctcgg cagcggcggc
      421 cgcttgccgt gcctcggcca accccggcgc cgctacttga gcgggcgctt gtgccggcac
      481 agtcgcgaca ctgcgcggca gcggagtccc ggtggatgga gactgtgagg gcgcgggcgc
      541 cggcgggttc gagacgggcg gggccgcggc cggcgcagca ggcgccgttc cggcctcgga
      601 gtgcgcgggc acctgcgcgg ggggtgcggc ggctggagct gccgcgtgcc ggacgagcag
      661 ccacgccacc accacaaggt tgacgacgaa cagtaccgcg atcgccgccg cccaggcggg
      721 aagcgcatgc cgcggcggtg cgaccttcac ctcgaagagc gccgggccgc tctgccgctg
      781 gcggtcactc tcggatttct tcaatgcgtc gaggatgaac gacatcgatc acccgccgtg
      841 agtatcgggc gtaaacaaga gcggcacgtc cggtccagcg accgcgctgg cgagcgcgat
      901 ctgcgtctcg cggccggcga tgccgtcgac cgacagctga tgctgacgct ggaagtcgcg
      961 cacgagtccg gcgagctcgg cgtcgaacac gtcgctcacc ggcgcgctcg actcgccgtg
     1021 cgcgagccgc tccaggctct ggcgcagcca gcgcacctcc gcgccgcgca tgccggccga
     1081 cagggccttg accggcgagc tcgccgggcg ccacagcatg acgaaatcac ccagccagaa
     1141 gtgcgacagc tcgcctaagc ccaccgcctg cgcgccgccg agatcgagcc gcgcgtgctg
     1201 atcgtccagt ccggtgagca cgacctggtg gctgctgccg cctgaatcgc tcagcatcag
     1261 tattgccgga tggttgtaga ggcgcagctg gccgaacgag ccgcgcgccg cgaggcactc
     1321 gaggtgcgcg cccgccgcct gggtacaggg gtcggtgccg ttcgcctggt atttggcgcc
     1381 ccagagcgca aagagcttgc cgaaggcgct gtcggtgtcg gtttcagcag cgtgcgcgcc
     1441 gagcagctgc gcgagggtcg gcaccggggg cgccgcggcg gcggctgcgg ccgacgtagc
     1501 cgggacggcc gcgatcaccg ccgtgtccgg cttcttgccc ggactccatg gcccgagcgc
     1561 ccacagtgag gcggttatca ccgccagcac ggcggcgacg accgcggcag ccgcccacgg
     1621 cagccagtac gcggcgaagc cgcgcccgaa aacttcggcg gccgcgcgcc gcaccagccg
     1681 gcccgtgacc cgttgccggt cgagcgaata ggcgccgagg agagcgcgat cgcagagcac
     1741 gttgatgacg cgcggcacgc cgcgcgacag gcggtacacg tcacgcagcg cccagggact
     1801 cacgatgtcg gtggtcgcac ccgcgacgcg caggcgatgg cgtacgtagg cggtggtctc
     1861 ctcgcgcccc agaggatcga ggtgatagcg gccggtgatg cgctgcgcga gctggcggag
     1921 ctccgtgcgc gcgagcagct cgcgcagctc cggctgtccg atgaggatga tctgcaggag
     1981 cttctgcgtg ttggtctcga ggttggtgag gaggcgcacc tgctccagca cctcgggtga
     2041 gagattctgc gcctcatcca ccaccagcac gacgcgcctg cctgcggcgt gcgcctcgag
     2101 caggtggccc gagagggtgt cgaccagata cttcaggctc tcgcgcgatt cgcccggtac
     2161 ctcgatgccg agctcctcgc agatggtcag gaggaactcc ggcgcggtca tcttcgggtt
     2221 gaggatgagg gcgatttcgg cgtccttcgg cgcctgcgcg aggagtgagc gcacgatggt
     2281 ggtcttgccg gtgccgacct cgccggtgag ctgcacgaag ccgcccgcct cgttgatgcc
     2341 gtacatgagg tgtgcgaggg cctccgcgtg gcgctcgctc aggtagaggt agcgcggatc
     2401 cggggtgatc gcgaacggct tctcgttcag gccgaaaaac ttgaggtaca tggtgccgga
     2461 tgctatccga gggtgtttcc gcgacgcagc gcccgcgcgc cgaagcgcgc ccgcgccccg
     2521 tcgagtgccg cgtcgaccgc ctgcgcgtgg cgcaggtcct cgaacaggcc gagctgggat
     2581 gcgggcttca gctctgtgag gaccacgccg aggaggcgaa gtttcgcctc cgggtgggac
     2641 tcgagccagc gctcgagaag ctcggccgcc accccggcaa tgacccgccc ggcagcggtg
     2701 gccggagcga tcgcgcgttg gcgggtgaag gtcgtgaagt cgtggcggcg gatcttgacc
     2761 ccgacgcaag cggtgaccag ctcgcggctg cgcagccgtg cgcacgccag gtccgccagg
     2821 cgcgcgagct gcacctgcaa cgcgcgcgca tcgccgatgt cctccgcgaa ggtgtcctcg
     2881 gcggagagag acttctcttc ggcgtccggc agcaccggcc gctcgtcgac gccggaggcg
     2941 cgctcgcgta cccaggcggt gtagcggccg aagagcggcc acaggaccgc atccggggcg
     3001 ctgcgcagct ccgcgagcgt gtggatcccg gccgcctcca cccgcgcgcc ggtcttgcgc
     3061 ccgagacccg ggagccgccg caccgagagc ggatcgagca ccgcgcgcac gcgttcgggc
     3121 tcaaccaccg acaggccgtc cggcttgccg agatcggagg cgatcttcgc cacgagcttg
     3181 ttgggcgcca cacccaccga ggcagtgagg ccggtgagct cacggatccg cgccttgatg
     3241 cggtgtgcga tctcgaccgc gccgcccatc agctcctggc tcgcggtgac gtcgaggaac
     3301 gcctcatcaa gagacagtcc ctcgaccagc ggcgtgacgt cacgaaacac gccgaacacc
     3361 gtacgcgaga cttcctggta gcgcgccatg cgcggacgca cgcagatcgc gtgcggacag
     3421 agccgcagag cggtgcgcat cggcatggcg gagcgcaccc cgaacgggcg cacttcgtag
     3481 ctggctgcgg tgacgacgcc ccgccctccg gtccagccga cgatgaccgg cttacccttg
     3541 atcgccgggt cgtcgtgctg ctcgacggac gcgtagaacg cgtccatgtc gacgtgcagc
     3601 accgcgcggc tcatatggcg accccctgag cggctcgcgc cctcagccgc tcactgctcg
     3661 acggtgatcg tgattttctt cgagatcacc ggcggatcgt gtgggatgtg gttctggtcg
     3721 gcgaggagca gttgcagcgt gtgcttgcct ggggtcaggg tgattgaggc ctcggtctgt
     3781 cccttgccga aatgcttgat gttgtcggtt gcggggagtg gcgcgctcag gttggcaggc
     3841 gcgtcggtgt cgatcagcag gtgatgatgc ccggtgttgt cgaatttcac gccggccggc
     3901 gcaacaccca tcttgtcgcc caggccgaac cgcaccagca ccgggctctt caccttggca
     3961 ccgtccttgg ggctgatgat gtagacctca gcgccggccg gtgagggagt gcgcggcatg
     4021 gcagccgcgg ccgtgggcgc ggctgccggc gcctgggcgt gagcgcgtgg cacagcggca
     4081 agggatgtgg cgaggactag tgcagcaacg atgaacgtac gcatcatgat ctcctttaga
     4141 gcgtgaggac gctgtacacc gtggtgagcg cgaccatcag cgcgccgctg atgagataga
     4201 agaagctgag cgccacgagc ttggcgaacg tgagccagcg gccctgcccg taaaccatcc
     4261 gcatggaacg atacacgtac cagggtatgt acaggcagag cgcgaagcgt acccagggtg
     4321 cggcccaagg caggagcgcc gacacccctg ccgcgagcag cagcagcagg aagccgaacg
     4381 cgtggttgtg cacgaacagc agcaggtgct cgacgtagta gtgtcgcggc caccagtaca
     4441 tgagcatcat gccggccgcg agcagcggca ggaacaggaa catggcccgc ggcacgttgt
     4501 gcagaaacgc ctcgcgcacc gagcgcccgt tgtc
//
LOCUS       DATLKL010000724         1625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_65198, whole genome shotgun sequence.
ACCESSION   DATLKL010000724 DATLKL010000000
VERSION     DATLKL010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65198"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1589
                     /gene="hutH"
                     /locus_tag="VM713_09775"
     CDS             51..1589
                     /gene="hutH"
                     /locus_tag="VM713_09775"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HVH84592.1"
                     /translation="MAEHDPLKLGARALKLAELRRIYAHPLTVAIDAGSLGAVTAAHT
                     ATARLAAAEAPAYGINTGFGLLAQTRIPAAQRALLQRNIILSHSCGVGPLLDDAVVRL
                     TLVLKLSSLLQGFSGVSEALVRFLERLINAGLMPCVPSQGSVGASGDLAPLAHLSLSV
                     LGLGEIRRHGEVLPAAEALAQEGLTPLALGPKEGLALLNGTQVSTALALAGLFELETA
                     FAAAVVSGALSVDALAGSDVPFDPRIHSVRGHGGQARVAQVYRELLEGSAIRDSHRDC
                     PRVQDPYSLRCQPQVMGACLDLIAQQAATLATEANAVTDNPLIFAASGEVLSGGNFHA
                     EPVAFAADILALAATEIASIAERRIALLVDPNMSGLPPFLAEESGVNSGFMLAQVTAA
                     ALVAENRMLCHPASVDSIPTSANQEDHVSMATHGARRLLGMTRNTLTVAAIEFLAAAQ
                     GIDFRAPLRTSPRLAGAHGALRLKVPFAKSDRLLALDIEEAIQTVRLPEVQSLARSLL
                     PSYR"
BASE COUNT          224 a          603 c          547 g          251 t
ORIGIN      
        1 atcggattca acccgcgccg gaccgtggtc cgcgcagccg aggttctgcg atggccgaac
       61 acgaccccct gaaactgggc gcacgggcgc tgaagctcgc ggagctgcgt cgcatctacg
      121 cgcatccgct caccgtggcg atcgacgccg gctcgctcgg cgcggtcacc gcggcgcaca
      181 cggccaccgc gcggctcgcg gcggccgaag cgcccgccta cggcatcaac acgggcttcg
      241 gcctcctcgc ccagacgcgc attccggcag cgcagcgcgc gctgctgcag cggaacatca
      301 tcctgtcgca cagctgcggc gtagggccgc tgctcgatga tgcggtagtg cgcctcaccc
      361 tggtgctgaa gctctcgagc ctgctgcagg gattttccgg ggtgagcgag gcgctggtgc
      421 gcttcctcga gcgcctcatc aacgcgggac tcatgccgtg cgtgccgagc cagggctcgg
      481 tcggcgcctc gggcgacctg gccccgctgg cgcatctgtc gctcagcgtg ctgggcctcg
      541 gcgagatccg ccgccatggc gaggtgttgc cagccgcgga agcgctcgcc caggaagggc
      601 tgacgccgct cgccctcggt cccaaggagg gactcgcgct cctcaacggc actcaggtct
      661 cgaccgctct cgccctggcg ggcctctttg agctcgagac cgcgttcgcg gccgcggtcg
      721 tgagcggcgc gctgtcggtc gacgcgctcg ccgggagcga cgtgcccttc gatccgcgca
      781 tccacagcgt gcgtgggcac ggcggtcagg cgcgcgtcgc ccaggtgtat cgcgagctcc
      841 tcgaaggcag cgcgatccgc gactcgcatc gcgactgccc gcgcgtgcag gatccctatt
      901 cgctgcgctg ccagccccag gtgatgggcg cgtgcctcga cctgatcgcc cagcaggcgg
      961 cgacgctcgc caccgaggcc aacgccgtca cggacaatcc gctcattttc gccgcctccg
     1021 gcgaagtgct ctcgggcggg aacttccacg ctgagccggt cgccttcgcc gccgacatcc
     1081 tcgcgctcgc ggcgaccgag atcgcctcga tcgccgagcg gcgcatcgcg ctcctggtcg
     1141 accccaacat gagcggcctg ccgccgttcc tcgccgaaga gagcggcgtc aattccggct
     1201 tcatgctggc gcaggtgacc gccgcggcgc tggtcgccga gaaccgcatg ctgtgccacc
     1261 cggcgagcgt cgacagcatt ccgacctctg ccaaccagga ggatcacgtc agcatggcaa
     1321 cgcacggggc gcggcggctc ctcggcatga cgcgcaacac gctcacggtg gccgccatcg
     1381 agttcctcgc ggccgcgcag ggtatcgact ttcgtgcgcc cctcaggacc tcaccaaggc
     1441 tcgccggcgc tcatggagcg ctgcggctca aggtgccgtt cgcgaagagc gaccgcctgc
     1501 tggcgctcga catcgaggag gcgatacaga ccgtgcgact tcccgaggtg cagtcgctcg
     1561 cgcgctcgct gctgccgagt tatcggtgat cgtcaggcgt agcgcgcgga cttctccggc
     1621 gcctt
//
LOCUS       DATLKL010000725         2356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_110178, whole genome shotgun sequence.
ACCESSION   DATLKL010000725 DATLKL010000000
VERSION     DATLKL010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110178"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="VM713_09780"
     CDS             <1..1509
                     /locus_tag="VM713_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84593.1"
                     /translation="DAALAAEQRATAAEAALAAARESLTAVEAERDGAQGAIQQGQEQ
                     YAELKAALEAGRARAEETQLSAARLERETTELGRERTVAATTLARAQQELAQATATAG
                     TLASQRTALDAARETCRSALGEARAAAQAAQLAAHDLHVQVETRRSTESSVAVGVNRM
                     LEQREQLAGRRATLEQELASGDEPIIQAQARLGEALERRLEVEAELAVARAALEEADA
                     ALRGLDEQRAAAELRVSAAREALEAARLAAQETHVRRAALAEQFAETRCELGEVLAGL
                     AAEANVGAWEQTLAETRAEVEKLGQVNLAAIDELKDQTQRKEYLDRQFADLTAALETL
                     AEAMRRIDRETRTRFEDTFERINAGLRDKFPRLFGGGHAYLELVGEDKLSAGVAVMAR
                     PPGKKNSTIHLLSGGEKALTAVALVFSIFDLNPAPFCLLDEVDAPLDEHNVGRFCDIV
                     RDMSSRVQFVFITHNKATMELASQLIGVTMLEPGVSRLVTVDVDEAVRLAAV"
     gene            1514..>2356
                     /locus_tag="VM713_09785"
     CDS             1514..>2356
                     /locus_tag="VM713_09785"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HVH84594.1"
                     /translation="MSELRWWLLILGAGFFAGLTFWELRRPRQAREREAPPEATRPRE
                     ATRTAPEATPPAEPPLHLPEMRALESLTSRGLPVLELPDDPDRFPVLEPAAEDADAAR
                     APVADRTAEASAAEPHPIEEQDSEYVFETENDLTPEPPAAAAKAPGVRDLLPELPAAP
                     APLVEWPPDAERQIVALRLVAPRPERFAGRSLRQALAAEGFVLGRFDIFHKPDETQRA
                     IVSAASLTRPGTFDPQTMDSQHFGGLSLFAVLPGPMSPPQAFEELVATARNLNDRLCG
                     VLQDE"
BASE COUNT          359 a          813 c          887 g          297 t
ORIGIN      
        1 gacgcggcgc tcgccgcgga gcagcgcgcc acggccgcgg aagcggccct cgccgcggcg
       61 cgtgagtcgc tcaccgcggt ggaggcggag cgcgacggtg cgcagggggc gatccagcag
      121 ggtcaggagc agtacgccga gctcaaagcg gcgctcgagg cgggccgggc ccgcgccgag
      181 gagacgcagc tgagcgcggc gcgtctcgag cgcgagacga cggagctagg gcgcgagcgc
      241 acagtcgcgg cgacgacact ggcgcgcgcg cagcaggagc tcgcgcaggc cacggccacc
      301 gccggcacgc tggcgtcgca acgcaccgct ctcgatgcag cccgcgagac ctgccgtagc
      361 gcgctcggcg aggcgcgtgc cgcggcgcag gccgcgcagc tcgcggcgca cgacctgcat
      421 gtgcaggtcg agacccgccg ctccacggag agctcggtgg cggtcggcgt gaatcgcatg
      481 ctcgagcagc gcgagcagct cgccggacgc cgcgccacgc tcgagcagga gctcgcctcg
      541 ggagacgagc cgatcatcca ggcgcaggcg cgcctcggcg aggcactcga gcggcgtctc
      601 gaggtggagg cggagctggc agtggcgcgt gcggcgctcg aggaagccga tgcggcgctg
      661 cggggcctcg atgagcagcg cgccgcggcc gagctgcgtg tgagcgcggc gcgggaagcg
      721 ctcgaggcgg cacgcctcgc cgcccaggaa acccacgtgc ggcgcgcggc gctcgccgag
      781 cagttcgccg aaacgcgctg cgagctcggt gaggtgctcg cggggctcgc cgcggaggcc
      841 aacgtcggcg cttgggaaca gacgctcgcc gagacacgcg ccgaggtcga gaagctcggc
      901 caggtcaacc tggccgcgat cgatgagctg aaggatcaaa cgcagcgcaa ggagtacctc
      961 gatcgccagt ttgccgacct caccgccgcg ctcgagacgc tcgcggaagc catgcggcgc
     1021 atcgaccggg agacgcgtac ccgcttcgag gacactttcg agcgcatcaa tgcggggctg
     1081 cgcgacaagt tcccacggct gttcggcggt ggccacgcct acctcgagct ggtcggcgag
     1141 gacaagctga gcgcaggcgt cgcggtcatg gcgcggccgc ccggcaagaa gaacagcacc
     1201 atccacctgc tgtcaggggg cgagaaggcg ctcacggcgg tggcgctggt gttctcgatc
     1261 ttcgatctca acccggcgcc attctgtctg ctcgacgaag ttgacgcgcc gctcgatgag
     1321 cacaacgtcg ggcgtttctg cgacatcgtg cgcgacatgt cctcgagggt gcagttcgtg
     1381 ttcatcaccc acaacaaggc caccatggag ctcgcctcgc agctgatcgg cgtgaccatg
     1441 ctcgagccgg gcgtgtcgcg cctggtgacg gtcgacgtcg acgaggcggt gcggctggcg
     1501 gcggtctgaa gcgatgtccg agttgcgctg gtggctgctg atcctcggag ccggattctt
     1561 cgccgggctc acgttctggg agttgcgccg cccgcgccag gcgcgcgaac gcgaggcgcc
     1621 accagaggcg acccggccgc gtgaggcaac ccggaccgcg cccgaagcga ctccgccggc
     1681 cgagccgccg ttgcatctcc cggagatgcg tgcgctcgag tcactgacga gccgcgggct
     1741 gccggtgctc gagctgccgg acgatcccga caggttcccg gtactggagc cggccgcaga
     1801 agacgcggac gcggcccgcg cacccgttgc ggaccgcacg gccgaggcga gcgccgctga
     1861 gccgcaccct atcgaggagc aggatagcga gtacgtcttc gagacggaga acgacttgac
     1921 gccggagccg cccgctgctg ccgctaaggc gccgggtgtg cgcgacctgc tgcccgaatt
     1981 gccggcggcg cccgcgccgc tggtcgagtg gccgccggat gcggagcggc agatcgtggc
     2041 gctgcgcctg gtggcgccgc ggccggagcg cttcgccggc aggtcgctgc gccaggcgct
     2101 cgcagccgag ggcttcgtgc tcggccgctt cgatatcttc cacaagcccg acgaaaccca
     2161 gcgcgcgatc gtgagcgctg cgagcctcac gcggcccggg accttcgacc cgcagaccat
     2221 ggactcgcag cacttcgggg gactgtcgct gttcgcggtg ctgccgggac cgatgtcgcc
     2281 gccgcaggca ttcgaggagc tggtcgccac cgcgcgcaac ctgaacgacc ggttgtgcgg
     2341 tgtgctgcag gacgag
//
LOCUS       DATLKL010000726         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_121604, whole genome shotgun sequence.
ACCESSION   DATLKL010000726 DATLKL010000000
VERSION     DATLKL010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121604"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VM713_09790"
     CDS             complement(<1..190)
                     /locus_tag="VM713_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVH84595.1"
                     /translation="MSEADPLIVFTPSGKRGRFPRGTQLLQAARTLGVDVDSVCGGRA
                     LCGRCQVLVMEGDFPKHGV"
     gene            complement(187..1131)
                     /locus_tag="VM713_09795"
     CDS             complement(187..1131)
                     /locus_tag="VM713_09795"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HVH84596.1"
                     /translation="MTDTVISSATKEVVLGLERPFVMIGERINPTGRKILAAEMAAGN
                     FSRVESDALAQIAAGAHMLDVNAGIPLADEPAILARAVQLVQSITDVPLSIDSSIVKA
                     LEAGLAVYKGKPLVNSVTGEEERLETVLPLVKKYGAAVVAISNDETGISEDPDVRFAV
                     AKKIVERAADHGIPACDIVVDPLVMPIGAINQAGRQVMHLLQRLKNELKVNTTCGASN
                     VSSGLPAREGISATFLCMAIGAGLTSAIMNPMHLEIVKACMAADVMMGHDPDCARWIR
                     RFREAPPLQASGAAAEAPQGAARGRREGGHRRRSRSPA"
     gene            complement(1128..2147)
                     /locus_tag="VM713_09800"
     CDS             complement(1128..2147)
                     /locus_tag="VM713_09800"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HVH84597.1"
                     /translation="MIFEDEPVPGYPLPILPGHTSPGRFERVLRAGQFAVTTELDPPD
                     SADPEDVYRRARVFDGYVDAINATDGSGANCHMSSMAVCALLTRMGYAPVMQMSCRDK
                     NRIAIQGDILGGAAMGVCNILCLTGDGVQAGDHPQALPVFDLDCMSLLETARTLRDEH
                     HFLSGRKITYAPRVLLGAAENPFAQPIEWRAQRLAKKVAAGAQFIQTQWCYDVPLLRA
                     FMRHVEELGLDGKVFILVGVGPLRSAKSAEWIRTHVPGVHIPDAVIKRMAGAADPAAE
                     GRRLCIELIQEIRTIKGVHGVHVMAFRQEHTVAEIIDRSGVLNGRVPWYPGRDPQTST
                     NRMAS"
BASE COUNT          340 a          776 c          798 g          361 t
ORIGIN      
        1 tcaccccgtg cttggggaaa tcgccctcca tgacgagcac ctgacagcgg ccacacagcg
       61 cccggccgcc gcacaccgag tcgacgtcga cgccgagggt gcgcgcggcc tgcagcagtt
      121 gcgtgccgcg cgggaagcgt ccgcgcttac ccgacggcgt gaagacgatc agtgggtcgg
      181 cctcgctcat gccgggctcc ggctgcgcct ccggtgtccg ccctcgcggc ggccgcgcgc
      241 ggcgccctgc ggcgcctcgg cggcggcgcc cgaggcctgg agcgggggcg cttcgcgaaa
      301 gcgccggatc caacgcgcgc agtcggggtc gtggcccatc atcacatccg ccgccatgca
      361 ggccttgacg atctcgagat gcatggggtt catgatcgcc gaggtgagcc ccgcaccgat
      421 cgccatgcag aggaaggtcg cactgatgcc ctcgcgcgcc ggcagcccgg agctgacgtt
      481 ggaggcgcca caggtggtgt tcaccttgag ctcgttcttc aggcgctgca gcaggtgcat
      541 cacctggcgg cccgcctggt tgatggcgcc gatcggcatg acgagcgggt cgaccacgat
      601 atcgcaggcc ggaatgccgt gatcggccgc gcgctctacg atcttcttcg cgacggcgaa
      661 gcgcacgtcc gggtcctcgg agatgccggt ctcatcgttg gagatcgcaa ccaccgcggc
      721 accgtacttc ttcacgagcg gcagcaccgt ctcgaggcgc tcctcttcgc cggtcacgga
      781 gttgacgagc ggtttgccct tgtagacggc gagcccggcc tcgagcgcct tgacgatcga
      841 ggagtcgatg gagagcggca cgtcggtgat cgactgcacg agctgcaccg cccgggcgag
      901 gatcgccggc tcatccgcga gcggaatacc cgcgttgacg tcgagcatgt gcgcgccggc
      961 ggcgatctgc gcgagcgcgt ccgattcgac gcggctgaaa ttccccgcgg ccatctcggc
     1021 cgcgaggatc ttgcgcccgg tcgggttgat gcgctcgccg atcatgacga agggtcgttc
     1081 gaggccgagc acgacctctt tggttgcgga gctgatcacg gtgtcggtca cgatgccatc
     1141 ctgttggtgc tggtttgcgg gtcccgcccc gggtaccacg gcacgcggcc gttgagcacc
     1201 ccggaacggt cgatgatttc ggccacggtg tgttcctggc ggaaggccat gacgtgcacg
     1261 ccgtgcacgc ccttgatggt gcggatctcc tggatgagct cgatgcagag ccggcgaccc
     1321 tcggcggcgg ggtcggccgc gccggccatc cgtttgatca cggcatcggg gatgtgcacg
     1381 cccggcacgt gcgtacggat ccactcggcg gacttcgccg agcgcagcgg tcccaccccg
     1441 accaggatga agaccttgcc gtcgagcccc agctcttcca cgtgccgcat gaaggcacgc
     1501 agcagcggca cgtcgtagca ccactgggtc tggatgaact gcgcgccggc ggcgactttt
     1561 ttcgcgaggc gctgcgcgcg ccactcgatg ggttgcgcga aggggttctc ggcggcgccg
     1621 agcagcaccc gcggtgcgta ggtgatcttg cggcccgaga ggaaatgatg ctcatcgcgc
     1681 agcgtccgcg cggtctcgag cagcgacatg caatccagat cgaacaccgg cagtgcctgc
     1741 ggatgatcgc ccgcctgtac gccgtccccg gtgaggcaga ggatgttgca cacgcccatg
     1801 gccgcgccgc ccaggatgtc gccctggatg gcgatgcggt tcttgtcgcg gcaggacatc
     1861 tgcatcaccg gcgcgtaacc catgcgggtg agcagcgcgc acaccgccat actcgacatg
     1921 tggcagttgg cacccgagcc gtcggtggcg ttgatcgcgt cgacgtagcc atcgaagacc
     1981 cgcgcgcggc ggtagacgtc ctccggatcg gcggagtcag gcgggtcgag ctcggtggtc
     2041 accgcgaact gtccggcgcg cagcacgcgc tcgaaccgcc cgggcgaggt atggcccggc
     2101 aggatcggca gcgggtagcc cggaaccggc tcgtcctcga agatcacggt gccgctccca
     2161 cgcgctcgcg caccacgcgc agccaggagg acttgccctg caggcgccga tcgatggcta
     2221 cctgcacgct ctcgagggac tgtgtgccgc cggggatgcg cgctgcgccc gcgac
//
LOCUS       DATLKL010000727         6739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_166735, whole genome shotgun sequence.
ACCESSION   DATLKL010000727 DATLKL010000000
VERSION     DATLKL010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166735"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..2028
                     /locus_tag="VM713_09805"
     CDS             34..2028
                     /locus_tag="VM713_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HVH84598.1"
                     /translation="MNRHIDTLLVANRGEIACRIFRTARRLGWRTVAVFSDADAPARH
                     VREADEALWIGPAPARDSYLSIERIIAAARACGAQAVHPGYGFLSENAGFAQACAEAR
                     ITFVGPPAEAIAAMGSKIVAKNRMRAAGVPVLPGYAGPDKDLKQLAAAAHTVGLPLII
                     KPAAGGGGKGMQIVRQESELTAGLAAARRLAESAFGDGALLLERFLPTPRHLEVQVFG
                     DTHGNYLHLGDRDCSAQRRHQKLIEEAPAPNVPAAVRERLRSAALVVAREIGYVSAGT
                     VEFLYDGREFYFMEMNTRLQVEHTVTEAVTGIDLVEWQLRLAAGEPLPLAQEAVRLDG
                     HAIEARVCAEDPERNFLPSAGRLALMEWPQLESVRVDAGFESGDTVPDCYDSLLGKVI
                     AWAPERSLAAERLALALNQTFSAGVHTNERWLARVLRSRRFLEVRHNIALLDKGAAEF
                     AGPSAVAPETLILAALALQGAPLGSDCSPWAARDGFTPNLPATIVCTLSWRGHSHTVE
                     LAYSGGKPVAANVDGRTLLQLSDLSYAAGAAGVGTIAARLVERRRHARCRVDGASVTL
                     WEEDAQYDFLLEDPRTREFTASASTGGLTTPLPGVVVSVPVSVGQKVAAGEVLMVIEA
                     MKMEHTITAPYAGTVQAIHFKRGDRVPEASQLLELARPEK"
     gene            2072..2623
                     /locus_tag="VM713_09810"
     CDS             2072..2623
                     /locus_tag="VM713_09810"
                     /inference="COORDINATES: protein motif:HMM:NF008437.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HVH84599.1"
                     /translation="MDKSLWTGLALSAAVATGAGAVAGLKLINRGPQFAEVVQVTPVT
                     RTVRTPRQECHDEQVTRQAPVQDEHQLLGTIAGAVVGGVIGHQVGGGTGRDLATVAGA
                     AGGGYAGNRIQKNLQDRDTVTSTARRCATVYDSAEKIVGYQVRYRLHGQEATVQMDHE
                     PGARIPLRDGRLDLTAAAPGKTG"
     gene            complement(2646..3338)
                     /locus_tag="VM713_09815"
     CDS             complement(2646..3338)
                     /locus_tag="VM713_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HVH84600.1"
                     /translation="MPTRIRSLTLAVLCVAAAALGTAAPARAQLVEGRDYRLLTPPRP
                     TSSPGKIEVVEFFSYACPHCARFSPLLSAWLATQPKDVAFRRVPVSYGRLQWMNLARA
                     YYALEASGDIKKLDTALFRAIHDGHEELFDEQSIADWVGKQGGDAARFADAYVSFGVN
                     NQTVQADAMAEDYAITAVPSLAVNGRYVVISPTEAADELQTFREVLQLADKVIALART
                     TTAGQAPAAKQR"
     gene            complement(3314..3910)
                     /locus_tag="VM713_09820"
     CDS             complement(3314..3910)
                     /locus_tag="VM713_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HVH84601.1"
                     /translation="MKCTKPARAFFARSALNVARELIGMHLVHDDGATVRRGRIVETE
                     AYLGPRDRAAHSSRGRTARTEVMFGPPGHAYIYFIYGFWNCLNVVTGAVGVPHAVLVR
                     ALEPLDGVTDKTWGPGLLCRAMHVDRRLNGADLRGSELWIERPQRAPSIAIGRSTRIG
                     VDYAGEWARRPWRFFDRASCYVSTIRRRPTECRPASAP"
     gene            3993..5021
                     /locus_tag="VM713_09825"
     CDS             3993..5021
                     /locus_tag="VM713_09825"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HVH84602.1"
                     /translation="MEYGKLGPRPAPWILGITAALCLSAPVGASVYPLPADGSPVVGT
                     DEAVTTVYQDTLPELAHRYSLGYYEIIRANPSVDVWLPGAGKRVVLPGRRILPSGPRE
                     GIVVNLPEHRLYYYPKPRSPHEQRVVITYPISIGKMDWRTPLGETRVIAKIRNPSWYP
                     PESIRKEHAANGDPLPKVVPAGPDNPLGAFALRLAAGDGEYMIHGTNNPTAVGMSVTH
                     GCIRMYPEDVAALFPLIAVGTKVWLVNEPVKVAWGEGELLLEAHPPVDEEGQSVEPDL
                     ELLSKLLDQALGSSTAAINWDLARKTLQAADGMPTVVGLAATLETPAAAADTPARLAN
                     GPAEAVKR"
     gene            complement(5018..5338)
                     /locus_tag="VM713_09830"
     CDS             complement(5018..5338)
                     /locus_tag="VM713_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84603.1"
                     /translation="MSRLKQLEARRRMLLRRSGEQREELAARLAELTPGAVLRGAVDA
                     AAIGTLRHPLAWAAALAGMLFLGRTREVLTFVLWVRSALALAGRAAHVVRLLTRLGAP
                     RAGP"
     gene            complement(5335..5733)
                     /locus_tag="VM713_09835"
     CDS             complement(5335..5733)
                     /locus_tag="VM713_09835"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HVH84604.1"
                     /translation="MSESAAPAGATSPDRPPPLQGLLATALDALRTRLELAAVELEIH
                     LIGVVRTLIWVVAAILCALLALAFGVVALIAALWDSHRVLGLISGGLVFLLLGAVCAY
                     VGARIFRRRPGILADSVEQLERDQRRAGGP"
     gene            complement(5730..6017)
                     /locus_tag="VM713_09840"
     CDS             complement(5730..6017)
                     /locus_tag="VM713_09840"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84605.1"
                     /translation="MTDVFNGGKLAEELRALVSEAEALLRTSSETAGAAGREHAEAAL
                     ADLRTRLTALEQQVKARARDVDVYVRDNPWQAVAVAGGVALLLGLIMGRRG"
     gene            6147..>6739
                     /locus_tag="VM713_09845"
     CDS             6147..>6739
                     /locus_tag="VM713_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HVH84606.1"
                     /translation="MNASVPNPLLELRKLGESAWLDDLGRGMLEDGSLARLIREDGIA
                     GVTSNPAIFAHSMTTDPRYAEPIARLLPTVPSAMALYEELAIGDVRAAARLLRGLYDE
                     SSGGDGFVSFEVSPHLADDADGSLAEARRLWARLDLPNAFIKIPGTEAGLPVIRTLIT
                     EGINVNVTLLFSPERYRAVTQAYMDGLSERARSRRPLG"
BASE COUNT         1067 a         2335 c         2375 g          962 t
ORIGIN      
        1 aaaacgcacg cccgcctggc aggagaagaa gtgatgaatc gccatatcga taccctgctg
       61 gtcgccaacc gcggcgagat cgcctgccgc atcttccgca cggcgcggcg cctcgggtgg
      121 cgtacggtcg ccgtgttctc cgatgcggac gcgcccgcgc gccacgtgcg cgaggcggac
      181 gaggcgctgt ggatcggccc ggcgccggcg cgcgacagct acctcagcat cgagcgcatc
      241 atcgcggcgg cgcgcgcctg cggcgcccaa gcggtccacc cgggttacgg ctttctgtcc
      301 gaaaatgccg ggttcgcgca ggcatgcgcg gaggcgcgga tcaccttcgt cggcccgcca
      361 gcggaggcga tcgccgcgat gggctcgaag atcgtcgcca agaaccgcat gcgcgcggcc
      421 ggcgtgcccg tgctgccggg ctatgcgggc cccgacaagg acctgaagca gctcgccgcc
      481 gctgcccaca cggtcggact cccgctcatc atcaagccgg ccgccggcgg cggcggcaag
      541 ggcatgcaga tcgtgcgcca ggagtccgag ctcaccgcag gcctcgccgc ggcgcggcgc
      601 ctcgccgaga gcgccttcgg cgatggggcg ctgctgctcg agcggttcct gccaacgccg
      661 cgtcatctcg aggtccaggt gttcggcgac acgcatggca actacctgca cctgggcgat
      721 cgcgactgct cggcgcagcg gcgccaccag aagctcatcg aggaggcgcc ggctcccaac
      781 gtccccgccg cggtgcgcga gcggctgcgc agcgcggcgc tcgtcgtggc gcgtgagatc
      841 ggttacgtga gcgccggcac cgtcgagttc ctctacgacg ggcgcgagtt ctacttcatg
      901 gagatgaaca cccggctgca ggtggagcac accgtcacgg aggccgtgac tggcatcgac
      961 ctcgtcgagt ggcagctgcg gctcgccgcg ggcgagccgc tccccctggc gcaggaggcg
     1021 gtgcggctcg atgggcacgc catcgaagcg cgcgtctgcg ccgaggatcc ggagcgcaat
     1081 ttcctcccga gcgccgggcg cctcgcgctc atggaatggc cgcagctcga gtcggtgcgc
     1141 gtggatgccg ggttcgagag cggcgatacc gtgcccgact gctatgactc gctcctcggc
     1201 aaggtgatcg cctgggctcc ggagcgcagt ctcgcggccg aacggctggc gctggcgctc
     1261 aatcagacct tcagcgccgg cgtgcacacc aacgagcgct ggctcgcgcg cgtgctgcgc
     1321 tcgcgccgct ttctcgaggt gcgccacaac atcgcgctgc tcgacaaggg cgcggctgaa
     1381 ttcgccggcc cgtccgcggt ggcgcccgag acgctgattc tggcggcgtt ggcgctgcag
     1441 ggcgcgccgc tcggcagcga ctgcagtccc tgggccgccc gcgatggctt cacgcccaat
     1501 ctcccggcga cgattgtctg cacgctctcg tggcgcgggc actcgcatac ggtcgagctc
     1561 gcctacagcg gcggcaagcc ggtcgcggcg aacgtggacg ggcggacgct cctgcagctc
     1621 agcgatctga gctatgcagc gggagccgcc ggcgtgggca cgatcgccgc gcggctggtc
     1681 gagcggcgcc gccacgcgcg ctgccgggtc gacggcgcct cggtcacgct ctgggaggag
     1741 gacgcgcagt acgattttct cctcgaggat ccgcgcacca gggaattcac cgcgagcgcc
     1801 agcaccggtg gcctcacgac cccgctccct ggcgtcgtgg tctcggtgcc ggtgtcggtc
     1861 ggtcagaagg tagccgcggg ggaggtgctc atggtgatcg aagccatgaa gatggagcac
     1921 accatcacgg cgccgtacgc gggcacggtg caggccattc acttcaagcg gggtgaccgc
     1981 gtgcccgagg ccagccagct gctcgagctg gcgcggccgg agaagtgacg cggtagaatt
     2041 ccgcgcatcc ctggcttagc aaggaggcga gatggacaag tcgttgtgga cgggactcgc
     2101 gctcagcgcc gcggtggcca ccggtgccgg cgcggtcgcg ggactcaagc tcatcaaccg
     2161 cggcccgcag ttcgcggaag tggtgcaggt gacgccggtg acgaggacgg tgcgcacccc
     2221 gcggcaggaa tgtcacgacg agcaggtgac ccgtcaggcg ccggtgcagg atgagcacca
     2281 gctactcggc acgattgccg gcgcggtggt cggcggagtc atcggccatc aggttggcgg
     2341 cggcaccggg cgcgacctcg cgaccgtcgc cggagccgcg gggggtggct acgcgggcaa
     2401 tcgcatccag aagaacctgc aggatcgcga taccgtcacg agcaccgcgc gcagatgcgc
     2461 aacggtctat gacagcgccg agaagatcgt cggctaccag gtgcgctacc ggctgcacgg
     2521 ccaggaggcg accgtgcaga tggatcatga gccgggggcg cgcattccgc tgcgtgacgg
     2581 gcggctcgac ctgacagccg ccgcccccgg caagacgggt tgaggacgcc tggcgcgcgc
     2641 gcccgtcagc gctgtttggc ggcgggcgcc tgccccgcgg tggtggtccg cgccagcgcg
     2701 atcaccttgt cggcgagctg cagcacttcc ctgaaggttt gcagctcgtc ggcggcctcg
     2761 gtggggctga tgacgacgta acggccgttg accgcgagcg agggaaccgc cgtgatcgca
     2821 tagtcctcgg ccatcgcgtc ggcctgcacg gtctggttgt tgacgccgaa tgacacgtag
     2881 gcgtcggcga accgggcggc gtcgcctccc tgcttgccga cccagtcggc gatcgactgc
     2941 tcgtcgaaga gctcctcgtg tccatcgtga atggcgcgga acagggcggt gtcgagcttc
     3001 ttgatatcgc cactcgcctc gagtgcgtaa taggcgcgcg cgaggttcat ccactgcaga
     3061 cggccgtagc tcaccgggac ccgcctgaag gcgacgtcct tcggctgcgt cgccagccag
     3121 gcgctcagca gcgggctgaa cctggcgcaa tgcgggcacg cgtaggagaa gaattccacg
     3181 acctcgatct ttccggggct cgaggtaggc ctgggcggcg tgagcaaccg atagtctcgt
     3241 ccttcgacga gctgcgcccg cgccggtgcg gcggtgccga gcgccgcggc ggccacacac
     3301 aggactgcga gagtcaggga gcggatgcgg gtcggcattc ggtcggtctc cgtcggatgg
     3361 tggaaacgta gcacgaagcg cggtcgaaga accgccaggg gcggcgcgcc cactcgcccg
     3421 catagtcgac gccgatgcgg gtgctgcgtc cgatcgcgat gcttggcgcg cgctgcgggc
     3481 gctcgatcca cagctcggaa ccgcggagat cggcgccgtt caagcggcgg tcgacgtgca
     3541 tggcgcggca gaggagcccc ggtccccagg tcttgtccgt gactccatcg agcggctcga
     3601 gcgcgcgcac gagcactgca tgcggcactc cgaccgctcc cgtcaccaca ttaagacaat
     3661 tccagaagcc gtaaatgaaa tagatatagg cgtgtcccgg aggaccgaac atcacctcgg
     3721 tgcgcgcggt tcggccgcgt gaggaatgcg cggcgcgatc gcgcgggccg aggtacgcct
     3781 ccgtctcgac gatgcggccg cggcgcacgg tggcgccgtc atcgtgcacc aggtgcatgc
     3841 cgatgagctc gcgtgcgacg tttagcgcac tgcgtgcgaa aaatgcgcgc gcaggcttgg
     3901 tacacttcac caccgcctga tgctaaagga atccctgcaa cgtcaaggac cggggtcgta
     3961 agcgagtgcg tttttgtagt aaaatcaacg ccatggaata tggcaagctt ggcccgcggc
     4021 cagctccctg gatcctcggt atcacggctg cgctctgcct gtcggcgccg gtcggcgcga
     4081 gcgtctatcc gttgcccgcc gatggctccc ccgtggtggg gacggacgag gccgtgacca
     4141 cggtgtacca ggacactctg cccgagctcg cccatcggta cagcctcggc tactacgaga
     4201 tcattcgcgc caaccccagc gtcgacgtgt ggctgccggg tgccggcaag cgcgtcgtgc
     4261 tgccgggacg gcgcattctc ccgtccggtc cgcgcgaggg catcgtcgtc aacctgcccg
     4321 agcatcggct ctattactac ccgaagccgc gcagccccca cgagcagcgc gtggtcatca
     4381 cctatccgat cagcatcggc aagatggact ggcgcacgcc gctcggcgag acgcgggtca
     4441 ttgccaagat ccgcaatcct tcctggtacc cgcctgagtc gattcgcaaa gagcacgccg
     4501 cgaatggcga tccgttgccg aaggtggtgc cggcgggtcc cgacaatccg ctcggggcat
     4561 tcgcgctgcg gctcgcggcc ggggacggcg agtacatgat ccacggcacc aacaacccga
     4621 ccgccgtcgg catgtcggtg acgcacggct gcatccgcat gtaccccgag gacgtcgccg
     4681 cgctgttccc gctgatcgcg gtcggcacca aggtgtggct cgtcaacgag ccggtcaagg
     4741 tggcgtgggg tgagggcgag ctgctgctcg aagcgcatcc gccggtggac gaggagggtc
     4801 agagtgtgga gccggacctc gagctcctct cgaagctcct cgaccaggcg ctcggcagca
     4861 gcaccgcggc gatcaactgg gacttggcgc gcaaaacgct gcaggcggcg gacggcatgc
     4921 cgaccgtggt cggactggcc gcgacgctcg agacgccggc agccgctgcc gacacgccgg
     4981 cccggcttgc gaacggaccc gccgaggccg tcaagcgtta gggacccgcg cgcggcgcgc
     5041 ccagccgcgt gaggaggcgc accacgtgcg ccgcgcgccc ggccagcgcc agcgccgagc
     5101 gtacccagag gacgaaggtc aacacctcgc gcgtgcgccc gaggaacagc atgccggcga
     5161 gcgccgccgc ccaggcgagc gggtgacgga gcgtgccgat ggcggcggca tcgaccgcgc
     5221 cacgcaggac agctcccgga gtgagctccg ccagccgagc ggcgagctcc tcgcgttgct
     5281 ccccggagcg ccgcaggagc atgcggcgcc gcgcttcgag ctgcttcagg cggctcacgg
     5341 cccaccggcc cggcgctggt cgcgctcgag ctgctcgacc gaatcggcaa ggatgcccgg
     5401 ccggcggcgg aagatgcgcg ccccgacata ggcgcacacc gcaccgagca gcagaaagac
     5461 gaggccgccg gaaatcaggc cgagcacgcg gtgcgagtcc cacagcgccg cgatcaacgc
     5521 cacgacgccg aaggcgagcg ccagcagtgc gcacagaatc gccgccacca cccagatcag
     5581 cgtgcgcacc acaccgatga gatggatctc cagctcgacc gccgcgagct cgagccgcgt
     5641 gcgcagcgcg tcgagcgctg ttgccagcag tccctgcagc ggcggggggc gatcgggcga
     5701 ggtcgcaccc gcgggcgcgg cgctctcgct catcccctgc ggcccatgat caacccgagg
     5761 agcagggcga ctccgccggc caccgcgacc gcctgccagg ggttgtcgcg cacgtagacg
     5821 tcgacgtcgc gcgcacgggc cttgacctgc tgctcgaggg ccgtcagtcg ggtacgcaga
     5881 tccgccagcg ccgcctcggc gtgctcccgc ccggccgcgc ccgccgtctc gctcgaggtg
     5941 cgcagcagcg cctcggcctc gctaaccagc gcgcgcagct cctcggccag ttttccaccg
     6001 ttgaatacat ccgtcatgag gggcgctcct tggaaaaaag tgtcaccgcg catgcctcca
     6061 gaagcctatg gggatttacg catatccggc ccgaaaccgc gagacggcgg ggtgaagctg
     6121 cgctactctc agggcggaag tgtccgatga acgcgtcggt tcccaatccg ctcctggagt
     6181 tgcgcaagct cggcgagagc gcgtggctcg atgatctcgg ccgcggcatg ctcgaggacg
     6241 ggagcctcgc ccgcctgatc cgcgaggacg ggatcgccgg tgtcacctcg aacccggcga
     6301 tcttcgccca ttccatgacc accgatccgc gctacgcaga gccgattgca cggctactgc
     6361 cgacggtgcc gtcggcgatg gcgctgtacg aggagctcgc catcggcgac gtgcgcgccg
     6421 cggccagact gctgcgcggg ctctatgatg agagctcggg aggcgacggc ttcgtgagct
     6481 tcgaggtctc gccgcacctc gccgacgacg cggacggcag tctcgccgaa gcgcgccgcc
     6541 tgtgggcacg actcgatctg ccgaacgcct tcatcaagat cccgggaacg gaggccggac
     6601 tcccggtgat ccgcacgctc atcaccgaag gtatcaacgt caacgtgacg ctcctgttct
     6661 cgccggagcg ctaccgcgcc gtgactcagg cctacatgga cggcctgagc gagcgcgccc
     6721 gcagccggcg gccgctcgg
//
LOCUS       DATLKL010000728         2557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_42642, whole genome shotgun sequence.
ACCESSION   DATLKL010000728 DATLKL010000000
VERSION     DATLKL010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42642"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VM713_09850"
     CDS             <1..1003
                     /locus_tag="VM713_09850"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HVH84607.1"
                     /translation="VAVFSFEYLLRVATARKRLAFVFSYQGIVDLLAIAPFYLAGLDA
                     RWLRALRLLRLLRVLKLQTHVLETTVAERTRELADKNAALEKAQAQLKAELEVARTLQ
                     LANLPAAFPVTPGCQGAARMIPATTMGGDFYDYIELPDGQIGLVIADVSGHGVPAAFF
                     MAVARTHLRELAARYTDPGACLAQTNDVLRAQNPLDLFVTVFYCILDPRTGVLSHANG
                     GHNPPYVRHAADPVEPLDGAGGLVLGAMPGVEYPTHAVQLLRGDRLVLYTDGVTEACN
                     ATAELYGARRLVDEVRVHGGGTPAALVERICQSVTDFAGSAPQSDDITLTVLTWGVP"
     gene            1000..1353
                     /locus_tag="VM713_09855"
     CDS             1000..1353
                     /locus_tag="VM713_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVH84608.1"
                     /translation="MNLRIEALAEATYVIPEGRLDFAAAPGFQEAIEQVMGGAARPPA
                     VIIDGAALEYVSSAGLRAVLLGARAAQRAGIPFALCALQPAVREVFELSGFTRIITVQ
                     ADRATALAQLHQGRP"
     gene            1350..1763
                     /locus_tag="VM713_09860"
     CDS             1350..1763
                     /locus_tag="VM713_09860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH84609.1"
                     /translation="MTAQRTVPADTSQLTVLNGFLQEFWTKAELEPAGLRSFELALEE
                     IFLNIAMHAPRPGNAPRVEVSLSLGADAVTMTVEDDGPQFNPLSLPPPDVTASLADRS
                     VGGLGVHLVRQVMDTVGYARVAGRNRLHLSKRLAG"
     gene            1945..2520
                     /locus_tag="VM713_09865"
     CDS             1945..2520
                     /locus_tag="VM713_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVH84610.1"
                     /translation="MRTNFLGAGRYFVKEGDDATLVLRCRKGERDAFERLVIRYQKPV
                     FNLALRMLRNRQDALDVAQTTFLKAFEHLGDYDPAFRFYSWLYRIAINECLNALTLRK
                     PRGELDANTPDEGPGPDSAAEGDQALHAIEDALMQISPEMRTVTVLRHITQLSYEDIA
                     ATLGLPEKTVKSRLHTARERLRDYLRQRGAI"
BASE COUNT          421 a          857 c          859 g          420 t
ORIGIN      
        1 cgtcgcggtt ttctcctttg aatacctgct gcgcgtcgcc accgccagaa agaggcttgc
       61 attcgtcttc agctaccagg gcatcgtgga cctgctcgcg atcgcccctt tctacctcgc
      121 gggtctcgat gcgcgctggc tacgggctct gcgactgctg cgactgctgc gcgtgctcaa
      181 gctgcagaca cacgttctcg aaaccaccgt cgccgagcgc acccgcgagc ttgctgacaa
      241 gaacgccgcg ctcgagaagg cgcaggcgca gctcaaagcc gagctggagg tggcgcggac
      301 actgcagctg gcgaacctgc cggcggcttt tccggtcaca cccggatgcc agggtgcggc
      361 ccggatgatc ccggcgacca ccatgggcgg ggatttctac gactatatcg agctccccga
      421 cggccagatc gggctcgtga tcgcggacgt gtccggccac ggcgtgccgg cggccttctt
      481 tatggcggtc gcacgcaccc acctgcgcga gctcgcggcg cgatacaccg accccggcgc
      541 ctgcctcgcg cagaccaacg acgtcttgcg cgcgcagaat ccgctcgacc tgttcgtgac
      601 ggtgttctac tgcatacttg acccgaggac cggcgtgctg agccacgcga acgggggcca
      661 caacccgccc tacgtgcggc acgctgcgga cccggtcgag cccctcgacg gcgcgggcgg
      721 gctcgtgctc ggtgcgatgc cgggggtcga gtatccaact catgcggtgc agctgctgcg
      781 aggtgatcgg ctggtgctct atacagacgg cgtgaccgag gcctgcaacg cgaccgcgga
      841 gctctacgga gcgcgccgac tggtcgatga ggtgcgcgtg cacggcggcg ggacgcccgc
      901 ggccctcgtc gagcgcatct gccagagcgt gacggatttc gccggctcgg caccgcagtc
      961 cgatgacatc accctcaccg ttctcacctg gggtgtgcca tgaacctgcg aatcgaggca
     1021 ctcgcggagg ccacctacgt gattccggag ggccggttgg acttcgcggc cgcgcccggc
     1081 tttcaggagg ccatcgagca ggtgatgggc ggcgccgcca ggccaccggc ggtcatcatc
     1141 gacggcgcgg cgctagagta tgtctccagc gccggactgc gggcagtcct gctcggggcg
     1201 cgggcggcgc agcgcgcggg catccccttc gcgctgtgtg cgctgcagcc ggcggtgcgc
     1261 gaggtgttcg agctgagcgg tttcacccgc atcataaccg tgcaagccga ccgggccacg
     1321 gcgctcgcac agctgcacca agggcgacct tgacggcgca gcgcacggtg ccggccgaca
     1381 catcgcagct caccgtgctg aacgggtttc tgcaggagtt ctggacgaaa gcggaactcg
     1441 agcccgcggg gctgcggagc ttcgagctgg cgctcgagga gatattcctc aacattgcga
     1501 tgcacgcccc ccggcccggc aatgccccgc gggtggaggt gtctctgagc ctcggcgcag
     1561 acgccgtcac aatgacggtc gaggacgacg ggccgcagtt caatccgctg tcgctgccgc
     1621 cacccgacgt gacggcgagc ctcgcggacc ggagcgtggg cgggctcggc gtacacctcg
     1681 tgcggcaagt gatggacacc gtcggctacg cgcgcgttgc cggccgcaac cggctgcacc
     1741 tgagcaagcg actcgccggc tgagcatatt cttcgtcctc cgccgaccac gtgcggccag
     1801 agtgaaaagc gcgtagcgta tcgctgacgg gaacgcctcg ggtccgggcg atgcccttta
     1861 taagaagagc accaaactgg gcctatcggg ggtagctgtg actcggctct gcgccgcgca
     1921 tcgtgctgcg gtgcgtcgcg tcgcgtgagg accaactttt tgggcgcggg gcggtatttc
     1981 gtgaaggagg gggacgacgc gacgctggtg ctgcgctgtc ggaagggtga acgggatgcc
     2041 tttgaacgac tcgtcattcg ctaccagaag cccgtcttca acctggctct gcggatgctg
     2101 cgtaaccgcc aggacgcgct cgacgttgct cagacgactt tcctcaaggc tttcgagcac
     2161 ctcggcgact acgatccggc gttccgcttc tacagctggc tctaccgcat tgcgatcaat
     2221 gaatgcctca acgcgcttac gttgcgcaag ccgcgtggcg agctcgatgc gaacaccccc
     2281 gatgaagggc cgggccccga cagcgctgcc gagggggatc aggcgctgca cgcgatcgag
     2341 gatgccctga tgcagatcag ccccgagatg cgcaccgtca ccgtcctcag gcatatcacg
     2401 cagctgagct acgaggacat tgcagcaacc ctggggttgc cggagaagac cgtgaaatcg
     2461 aggctgcaca ctgcgcggga gcgcctgcgc gattacctcc gacagcgcgg agccatctga
     2521 cgtgaccgac gacgaccgca tggcactcat tcacgcc
//
LOCUS       DATLKL010000729         2035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_189371, whole genome shotgun sequence.
ACCESSION   DATLKL010000729 DATLKL010000000
VERSION     DATLKL010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189371"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VM713_09870"
     CDS             complement(<1..932)
                     /locus_tag="VM713_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVH84611.1"
                     /translation="MSDAPSGARVAEAPAAPLAALFATRRRSLVQASFVTIYLVWGVS
                     YAVGRIMATELPPLLAAGVRFCFSAALLTLLVYASGLALPVRARDWRLIVAAALLGIA
                     VSNGLNVLALRHVASSQVALISASSAFWIAWIGRYGRHPTPVSKRTWLGLFVGFVGVA
                     ILVSAHGVGLHARFAWQLVVLGSALAWALATAAIRESQPECDPLAFTASYLLVGGVAL
                     VAMGLATGDAGRWSWSPRGLAALVFLAIFSSTLAFLAYTYLLRHEPPARIGTYAYVNP
                     LVAVFAGWALLDERLNARQWAGTAIIFAGVVLVR"
     gene            complement(932..>2035)
                     /locus_tag="VM713_09875"
     CDS             complement(932..>2035)
                     /locus_tag="VM713_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVH84612.1"
                     /translation="PLDLLAETTALEPERFARASAAPQPEAAVIQAARELLDRAQRPL
                     IVAGGGARHAGAALLQLLEALDGYLVTTVAAKGVIPESHPANLGASLPYRATQELLAA
                     ADVVLAAGTELAETDVYTTTRLTFPGRVIRVDIDAAQLADPSAQAVALHADARTALEL
                     LARAVHARRGWRSAAGEARSARLAVEAQIGTHSRPALGAVRALRAAVPADGVVFSDMT
                     QIAYTGNYAFAADRPGSWFHPSGYGTLGFALPAAIGAKVAQPERAVVALAGDFGVQFT
                     LQELVTAVELQLPLPVVVWNNEALAQIRDDMLAAGIEPVGVAGRNPDFQALAAACGAA
                     GCRVRGPEALTQGVRAALEARGPTLLEARATDF"
BASE COUNT          322 a          707 c          764 g          242 t
ORIGIN      
        1 ttgcgcacca gcactacgcc cgcaaagatg atagcggtgc cggcccactg ccgtgcgttc
       61 agccgctcat cgagcagtgc ccagccggcg aataccgcga ccagcggatt gacgtaggcg
      121 taggtgccga tccgcgccgg cggctcgtgg cgtagcagat aggtgtacgc gaggaatgcg
      181 agtgtcgagc tgaagattgc caggaacacc agcgccgcca ggccccttgg cgaccatgac
      241 cagcgcccgg catccccggt tgcgagcccc atggccacga gggccacgcc gcccaccagc
      301 agatagctgg cggtgaaggc gagcgggtcg cactccggct gtgattcgcg aattgccgcg
      361 gtggcgagcg cccaggcgag cgcgctgccg agtaccacca gctgccaggc gaagcgcgcg
      421 tggagcccga ctccgtgcgc cgagaccagg atcgccacgc cgacgaagcc cacgaacagg
      481 ccgagccagg tgcgcttgct caccggagtc gggtgacggc cgtacctgcc gatccacgca
      541 atccaaaagg cggagctggc gctgatgaga gcgacctggc tcgaagcgac atggcgcagc
      601 gcgagcacgt tgaggccgtt gctcaccgcg ataccgagaa gcgcggcggc gacgatcagg
      661 cgccagtcgc gagcgcgcac cgggagcgcg agaccgctgg catagacgag gagcgtgagg
      721 agcgctgccg agaagcagaa gcgcaccccg gccgccagta gcggcgggag ctcggtggcc
      781 atgatgcggc cgaccgcgta gctgacaccc cacacgaggt agatggtgac gaatgaggcc
      841 tgtaccagcg agcggcggcg tgtcgcgaac agcgccgcca gcggcgcagc gggcgcctcg
      901 gcaacgcggg cgccggacgg cgcatcgctc atcagaagtc cgtcgcgcgc gcctcgagga
      961 gcgtagggcc ccgggcctcg agcgccgcgc gcacgccctg agttagtgcc tcgggcccgc
     1021 gcaccctgca tcccgcggcg ccgcaggcgg cggcgagggc ctggaagtcg ggattgcggc
     1081 ccgccacccc gaccggctcg atgccggcgg cgagcatgtc gtcgcggatc tgcgcgagtg
     1141 cctcgttgtt ccacaccacg accggcagag gcagttgcag ctcgactgcg gtcacgagct
     1201 cctgcagcgt gaactgcacg ccaaagtcgc cggccagagc gacgaccgct cgctcaggct
     1261 gggcgacctt ggctccgatg gcggccggca gtgcaaaacc cagcgtgccg tagcccgagg
     1321 ggtgaaacca actccccggt cgatcggcgg cgaaggcgta gttgcccgtg taggcgatct
     1381 gggtcatgtc gctgaagacg acgccgtcgg cgggcaccgc ggcgcgcaag gcccgcaccg
     1441 cgccgagggc cgggcgtgag tgcgtgccga tctgcgcttc gaccgcgagc cgagcgctgc
     1501 gcgcctcgcc cgcggcgctg cgccagccgc gccgggcgtg cacggcccgc gccaggagct
     1561 cgagcgcggt gcgcgcatcg gcgtggagcg cgaccgcctg tgccgacgga tcggcaagct
     1621 gcgctgcgtc gatgtcgacg cggatgacgc ggcccggaaa ggtgagccgg gtcgtggtgt
     1681 agacgtcggt ctcggcaagc tcggtgcccg cggcgagcac cacgtccgcc gcggcgagga
     1741 gctcctgcgt ggcgcggtag gggaggctgg cgccgagatt cgcggggtgg ctctcgggga
     1801 tgacgccctt ggccgcgacg gtcgtaacca gatagccgtc gagcgcctcg agcagctgca
     1861 gcagcgccgc gcccgcgtgg cgtgcgccgc cgcccgcgac gatgagcggc cgctgcgctc
     1921 ggtcgagcaa ctcgcgcgcc gcctggatga ccgcggcctc cggctgcggc gcggcgctcg
     1981 cgcgcgcgaa gcgctccggc tcgagcgccg tcgtctccgc gagcaggtcg agagg
//
LOCUS       DATLKL010000730         1931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_177931, whole genome shotgun sequence.
ACCESSION   DATLKL010000730 DATLKL010000000
VERSION     DATLKL010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177931"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..940
                     /locus_tag="VM713_09880"
     CDS             68..940
                     /locus_tag="VM713_09880"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HVH84613.1"
                     /translation="MDVTLLKVIVLAIVQGLAELLPVSSSAHVVVAEKLMGLDPSSPP
                     MTLLLVMLHTGTMLAVIVYFWREWRALYFQSAAAFRSFLWLIAVATFFTAVIGEGIVK
                     LIERTVLRDVPHAQIEDLFSHLEFIAPALAAAGILIVIAGILERRRPVAGHGVAPDLD
                     TRRAGVIGAVQGLCLPFRGFSRSGATISAGMLLGVAKPRAEAFSFALAVVLTPPVVAR
                     EVMRLRDAAHAGGTDLGSVALLTIIGAVIALIAGLLALKWLSRWLESGRWYLFGIYCL
                     AAAAAVTLLHRAGY"
     gene            complement(975..>1931)
                     /locus_tag="VM713_09885"
     CDS             complement(975..>1931)
                     /locus_tag="VM713_09885"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008137.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HVH84614.1"
                     /translation="LTINAMAESDTGEIIDPYGGRADLAAGVLHHVADSFTEDPVRVL
                     RAARFAARFAGRGFRIADETMQLMRRMTASGEVNALTPERVWQETERALAEDHPEVFF
                     ATLRECGALKVIYPEIDALYGVPQPPKWHPEIDTGVHVMLALAWAARARLSTAVRFAV
                     LMHDLGKARTPRELWPKHHGHEEAGVPLIEALADRLRVPGTHRELAILAAREHTLVHR
                     ALELRPETVLKLLQECDAFRRSERFAELLQACEADARGRAGLEANPYPQAPYLRAALT
                     AAAAVTLSESERRGLNGPAIGAEMRRRRLAAVSAVKAAAPAA"
BASE COUNT          250 a          666 c          692 g          323 t
ORIGIN      
        1 accgctcggg ggctttgcgc tgcaggacct gcgccgcctg ctgtgaggtt gcggttaaga
       61 tgtgtgcatg gacgtgacac tgctcaaggt catcgtgctc gcgatcgtcc aggggctcgc
      121 ggagctgctg ccggtgtcga gctcggcgca cgtggtggtc gcggagaagc tcatgggcct
      181 cgacccctcc tcgcccccga tgacgctgct gctcgtcatg ctgcataccg gaaccatgct
      241 cgccgtgatc gtgtatttct ggcgcgagtg gcgcgcgctc tattttcaga gcgccgcggc
      301 cttccgcagc ttcctctggc tgatcgcagt agcgaccttc ttcaccgcgg tgatcggcga
      361 gggcatcgtg aagctgatcg agcggaccgt gcttcgcgac gtgccgcacg ctcagatcga
      421 ggatctcttc agccatctcg agttcatcgc cccggctctc gcggccgccg gcatcctgat
      481 cgtgatcgcg gggatcctcg agaggcgccg tcccgtagcg gggcacggcg tggcgccgga
      541 tctcgatacc cggcgcgccg gggtcatcgg ggcggtgcag gggctgtgcc tgccgtttcg
      601 cggcttctcg cgctcggggg ccacgatctc cgccggcatg ctgctcgggg tcgcgaagcc
      661 gcgggcggag gccttcagct tcgcgctcgc cgtggtgctg actcctccgg tggtcgcgcg
      721 cgaggtcatg cgtctcaggg acgccgcgca cgcggggggc actgacctcg gctcggtcgc
      781 actgctcacg atcatcggcg cggtgatcgc gctgatcgcc ggactcctcg cgctcaaatg
      841 gctgagtcgc tggctcgaga gcggccgctg gtatctgttc gggatctatt gcctcgccgc
      901 ggccgccgcc gtgacgctgc tgcaccgggc cggttactga gcccactgcg gggcaggcgg
      961 gaatctccgg cgactcaggc cgcgggcgcg gccgctttga ccgcgctcac cgccgcgagc
     1021 cgccgccgcc tcatctctgc gccgatagcc ggaccgttga gcccgcggcg ctcgctctcg
     1081 gagagcgtca cggcagcggc cgcggtgagc gccgccctga gatagggcgc ctgggggtaa
     1141 gggttggcct ccaagccggc gcggccacgg gcgtccgcct cgcacgcctg caacagctcc
     1201 gcgaagcgct cggaacggcg gaaggcgtcg cactcctgca gcagcttcaa cacggtctca
     1261 gggcggagct cgagcgcacg gtgcaccagc gtgtgctcgc gcgccgcaag gattgcgagc
     1321 tcgcggtgcg tgcccgggac cctgaggcga tcggcgaggg cctcgatcag cggcacgccc
     1381 gcctcctcat ggccgtgatg cttcggccag agctcgcgcg gcgtgcgcgc cttgccgaga
     1441 tcgtgcatga gcaccgcgaa gcgcaccgcg gtggacagac gggcgcgagc cgcccaggcg
     1501 agtgccagca tgacgtgcac gcccgtgtcg atctccgggt gccacttagg cggctgcggc
     1561 acgccgtaga gcgcgtcgat ttcgggatag atcaccttga gcgcgccgca ctcgcgcagc
     1621 gtggcaaaga atacctccgg gtggtcctcg gcgagcgcgc gctcggtctc ctgccacacg
     1681 cgctcgggcg tgagtgcatt cacctctccg gaggccgtca tgcgccgcat gagctgcatg
     1741 gtctcgtccg cgatgcgaaa gccgcggccg gcgaagcgcg ccgcgaagcg cgccgcgcgc
     1801 agcacgcgca ccgggtcctc agtgaaactg tccgcgacgt ggtgcagcac gccggcagcg
     1861 aggtcggccc ggccaccgta ggggtcgatg atctcgccgg tgtcgctctc ggccatggcg
     1921 ttgatggtga g
//
LOCUS       DATLKL010000731         1555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_19926, whole genome shotgun sequence.
ACCESSION   DATLKL010000731 DATLKL010000000
VERSION     DATLKL010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19926"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VM713_09890"
     CDS             complement(<1..504)
                     /locus_tag="VM713_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HVH84615.1"
                     /translation="MRRPRLEVADVFHRHGAAWRRANAGHVSLGQLQVMSAIEQCRSA
                     ALGGHVERCEDCGHSRIAYNSCRNRHCPKCQGAAAQDWLAAREADLLPVGYFHVVFTL
                     PAEIAPIAYQNKAIVYDLLFRTAAETLLTIAADPKHLGARIGATAVLHSWGATMTHHP
                     HVHMIVPG"
     gene            complement(515..1375)
                     /locus_tag="VM713_09895"
     CDS             complement(515..1375)
                     /locus_tag="VM713_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVH84616.1"
                     /translation="MIEDMTVRGFNEHTRRDYVRHVRSFAAFIGRSPDTASAEDLRLF
                     QLHQTRIGMQPPSINSAVSALRFFFTVTLDRPDLSRRLTVVPQPRRIPAVLSVEEVTL
                     LLGAATAPKYKAAFATAYGAGLRVSEVVALKVGDIDSERMLLRVERGKGRKDRHAMLS
                     PQLLELLRAWWREGRRRSLLLPGGWLFPGRNPVEPLSARQLCRAVRAAAQAAGIKKRV
                     SPHTLRHSFATHLLEQDVDIRVIQTLLGHAKLDTTALYTRVANTTIRAVTSPLDRLAP
                     LIDGRARSGT"
BASE COUNT          254 a          505 c          550 g          246 t
ORIGIN      
        1 acccggcacg atcatgtgga catgcggatg gtgggtcatc gtcgcgcccc aactgtggag
       61 cacggcagtg gcgccgatgc gggcgccgag gtgtttggga tcggcggcaa tagtcagcag
      121 cgtctcggcg gccgtgcgga acagcaggtc gtagacgatc gccttgttct ggtaggcgat
      181 gggagcgatc tcggcgggca gcgtgaagac cacgtggaaa tagccgaccg gcagcagatc
      241 ggcctcgcgc gccgcgagcc aatcctgcgc cgctgcgccc tgacactttg ggcaatggcg
      301 attgcggcat gagttgtagg cgatgcggct gtggccgcaa tcctcgcagc gctcgacatg
      361 cccaccgagc gcggccgagc ggcactgctc gattgccgac atgacctgga gctgacccag
      421 gctcacatgc ccggcattgg cccggcgcca agcggctcca tggcgatgaa agacgtctgc
      481 gacctccagc ctcgggcggc gcacggccgc ccggctacgt gccggatcgc gctctcccgt
      541 cgatcaatgg tgccaaccgg tcgagggggc tggttacagc ccggatcgtg gtattggcga
      601 cgcgagtgta gagcgctgtg gtgtcgagtt tggcatggcc cagaagtgtc tgaatcacgc
      661 gaatatcgac atcttgctcg aggaggtggg tggcgaaact gtgccgcagt gtgtgtggcg
      721 acacgcgctt cttgatcccc gcggcctggg cggcggcacg gacggcgcgg cacagctggc
      781 gggccgacag cggctcgacc gggttgcggc cgggaaacag ccagcctccc ggcagcagca
      841 ggctgcgccg ccgcccctcc cgccaccagg cgcgcagcag ctcgagcagc tgcggcgaga
      901 gcatcgcgtg gcggtccttg cgccccttgc cgcgctcgac gcgcagcagc atgcgctcgg
      961 aatcgatatc gccgaccttg agcgcgacca cctccgagac gcgcagcccg gcgccatagg
     1021 cggtggcgaa cgccgccttg tactttggcg ctgttgccgc gcccagcagc agtgtcacct
     1081 cctcgacgct gagcaccgcc ggtatccgcc gcggctgcgg cacgacggtg agccggcgcg
     1141 acaggtccgg ccggtcgagc gtcaccgtga agaagaagcg cagagccgag accgcgctgt
     1201 tgatgctcgg cggctgcatg ccgatccgcg tctggtgcag ttggaagaga cgtaggtctt
     1261 ccgccgacgc cgtgtcgggt gagcggccga tgaaggccgc gaatgaccgc acgtggcgga
     1321 cgtaatcgcg gcgcgtgtgc tcattgaaac cgcgcacggt catgtcttcg atcatgcgcg
     1381 cccgcaaggg gctgacgggc cgaagtgaag tatcggaggt catcggaggc cttctcctga
     1441 accgaaggtt ggtggacctt cgatgttcgg gatcggacgc tccggccgtc acgcgctacc
     1501 gcacatcacc tcgtcgaaaa tgcgccgacc gcgacgcgtg ctccctcccg cgcga
//
LOCUS       DATLKL010000732         2900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_8580, whole genome shotgun sequence.
ACCESSION   DATLKL010000732 DATLKL010000000
VERSION     DATLKL010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8580"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..222
                     /gene="rplC"
                     /locus_tag="VM713_09900"
                     /pseudo
     CDS             1..222
                     /gene="rplC"
                     /locus_tag="VM713_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="binds directly near the 3' end of the 23S rRNA,
                     where it nucleates assembly of the 50S subunit; essential
                     for peptidyltransferase activity; mutations in this gene
                     confer resistance to tiamulin; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
     gene            313..918
                     /gene="rplD"
                     /locus_tag="VM713_09905"
     CDS             313..918
                     /gene="rplD"
                     /locus_tag="VM713_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HVH84617.1"
                     /translation="MKLKSVNGGATVQVSEAAFGHKFNEALVHQVLTAYRNAGRAGTK
                     AQKSRAEVSGGGRKPYAQKGTGQARAGSNRSPIWVGGGKTFAAKPRSFEQKVNRKMYR
                     GALRSMLSELARTERLLVTDGIQLSEPKTKLLAHQLKEWSLPSVLIVVEATDQKLALA
                     ARNLPHVEVLEVTALNPVSLAAYDKVLMTVGAVKLIEERLQ"
     gene            915..1217
                     /gene="rplW"
                     /locus_tag="VM713_09910"
     CDS             915..1217
                     /gene="rplW"
                     /locus_tag="VM713_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004145338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HVH84618.1"
                     /translation="MNREQLMSVLIAPHVTEKTSLAMQNHNQYTFRVRRDATKTDIRK
                     AVELMFDVKVAGVQVVNEPGKQRRFGRIAGRTQDWRKAYVSLTPGQTIDYEARAKA"
     gene            1232..2068
                     /gene="rplB"
                     /locus_tag="VM713_09915"
     CDS             1232..2068
                     /gene="rplB"
                     /locus_tag="VM713_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HVH84619.1"
                     /translation="MALIKYKPRTPGTRAVVKVDRSHLYKGGPRMQLTAHQYKTGARN
                     HFGRITTRHKGGGSGQKYRVIDFKRDKDGITGVIERLEYDPNRTSHIALIKYPDGEWR
                     YILAPKGVKEGDEVRSGADAPIKPGNAMSLRHVPVGSIIHNVELKPGRGGQLARSAGS
                     SVSFTAREGIYAYVRLKSGETRKVHIDCRATIGEVGNDEHNLRSYGKAGAKRWRGIRP
                     TVRGLAMNPVDHPHGGGEGKSGQGNPHPVSPWGWNTKGLKTRHNKRTDNMIVQRRKNR
                     IR"
     gene            2083..2376
                     /gene="rpsS"
                     /locus_tag="VM713_09920"
     CDS             2083..2376
                     /gene="rpsS"
                     /locus_tag="VM713_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HVH84620.1"
                     /translation="MPRSLKKGPFVDLHLAKKVQAASAKNDRKPIKTWSRRSMIVPEM
                     VGLTIAVHNGRQHVPVLVTENMVGHKLGEFAPTRTFKAHSGDRKVEAAPAAAT"
     gene            2399..2740
                     /gene="rplV"
                     /locus_tag="VM713_09925"
     CDS             2399..2740
                     /gene="rplV"
                     /locus_tag="VM713_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HVH84621.1"
                     /translation="MQVTSKLRYARISPQKCRLVADVVRGKPVGNAIATLKFMPKKGA
                     KLVRKVLESAVANAEHNHGADIDLLKVSLIHVDAAPQFKRFAARAKGRGTRIIKRNSH
                     ITVGVSDGRKD"
BASE COUNT          579 a         1000 c          942 g          379 t
ORIGIN      
        1 aactcgctgt cgcaccgtgc gccgggctcg atcggccagc gtcagacgcc gggccgcgtg
       61 ttcaagggac gccgcatgtc ggggcacatg ggcgtcgtac ggcggaccac cgagaacctg
      121 cgcgtcgtgc aggtcgatgt cgagagaaac ctgctcctga tccgcggcgc ggtgccggga
      181 gtcgagggcg gacaggtgat cgtgcgcccc tcgctcaagg ccgggcgacg cgccctccgc
      241 aagacccttg ctccctcgaa ggccggcgcc gctccgtcca aggacccggc gaagcaggga
      301 aagaaataag ccatgaagct caagtcagtg aacggcggag cgacggttca ggtgtccgaa
      361 gccgccttcg gccacaagtt caacgaggct ttggtgcatc aggtactcac cgcctaccgc
      421 aacgcaggcc gcgccggcac caaggcgcag aagtcgcgtg ccgaggtgtc gggcggcggc
      481 aggaagccct acgcgcagaa gggcaccggc caggcgcgtg cgggctccaa ccgctcgccc
      541 atctgggtcg gcggcggcaa gaccttcgct gccaagcccc gcagcttcga gcagaaggtg
      601 aaccgcaaga tgtatcgcgg cgcgctgcgc tcgatgctct ccgagctcgc gcgcaccgag
      661 cggctgctgg tgacggacgg catccagttg agcgagccga agaccaagct gctcgctcat
      721 cagctgaagg agtggtcgct gccctcggtg ctgatcgtgg tcgaggcgac cgaccagaag
      781 ctcgccctcg ccgcgcgcaa tctcccgcac gtggaggtgc tcgaggtgac cgcgctcaat
      841 cccgtctcgc tcgccgccta cgacaaggtg ctgatgacag tcggcgccgt gaagctcatc
      901 gaggagcgcc tgcagtgaac cgtgagcagc tgatgagcgt gctgatcgct ccgcacgtga
      961 ccgagaagac ctcgctcgcg atgcagaacc acaaccagta cacgttccgc gtgcgccgcg
     1021 acgcgaccaa gaccgacatc aggaaggccg tcgagctcat gttcgacgtc aaggtggccg
     1081 gcgtccaggt ggtcaacgag ccgggcaagc agcgccgctt cggccgcatc gccgggcgta
     1141 cgcaggactg gaggaaggcg tacgtgagcc tcacgcccgg tcagaccatc gactacgaag
     1201 cccgcgccaa ggcctgagag gaccgaccgc aatggctctc atcaagtaca agccccgcac
     1261 tcccggcacg cgcgcggtcg tcaaggtgga ccgctcgcac ctctacaagg gcgggccgcg
     1321 catgcagctc accgcccacc agtacaaaac cggcgcccgc aaccacttcg gccgcatcac
     1381 cacgcgccac aagggcggcg gctcgggcca gaaataccgc gtcatcgact tcaagcgcga
     1441 caaggacggc atcaccggcg tcatcgagcg gctcgagtac gatccgaacc gcaccagcca
     1501 catcgcgctc atcaagtacc ccgatggcga gtggcgctac atcctcgcac ccaagggcgt
     1561 gaaggaaggc gacgaggtgc gctcgggggc cgatgcgccg atcaagccgg gcaacgccat
     1621 gtcgctcagg cacgttccgg taggctcgat catccacaac gtcgagctca agcccggccg
     1681 cggcgggcag ctcgcccgca gcgccggcag ttccgtttcc ttcaccgccc gcgaaggcat
     1741 ttacgcgtac gtgcggctca agtccggcga gacgcgcaag gtgcacatcg actgccgcgc
     1801 gacgatcggc gaggtaggga acgacgagca caacctgcgc agctacggca aggcgggagc
     1861 caagcgctgg cgcggcatcc gcccgacggt gcgcggactc gccatgaacc cggtcgacca
     1921 cccgcacggt ggcggcgagg gcaagtctgg ccagggcaac ccgcacccgg tgtcgccgtg
     1981 gggctggaac accaagggac tcaagacccg ccacaacaag cgcacggaca acatgatcgt
     2041 ccagcgccgc aagaaccgca ttcgctgatc ggagcgcact aaatgccacg ctcattgaag
     2101 aagggaccgt tcgtcgacct gcacctcgcg aagaaggtac aggcggcgtc cgccaagaac
     2161 gaccgcaagc cgatcaagac ctggtcgcgc cgctcgatga tcgttccgga gatggtggga
     2221 ctcaccatcg ccgtgcacaa cggccgccag cacgtgccgg tgctcgtcac cgagaacatg
     2281 gtgggccaca agctcggcga gttcgccccc actcgcacct tcaaggcgca ctcgggcgac
     2341 cgcaaggtcg aggcggcgcc ggccgccgcg acctgaggcc gccacgggca ggaaccacat
     2401 gcaagtcacc tctaaactcc gctacgcgcg catctctccg cagaaatgcc ggctggttgc
     2461 ggacgtcgtg cgcggcaagc cggtcggcaa cgcgatcgcg accttgaagt tcatgccgaa
     2521 gaagggcgcc aagctcgtgc gcaaggtgct cgagtccgcg gtcgccaacg ccgagcacaa
     2581 ccacggcgcc gacatcgacc tcctcaaggt gtcgctcatc cacgtcgacg cggccccgca
     2641 gttcaagcgc ttcgcggcgc gcgccaaggg acgcggcacg cgcatcatca agcgcaacag
     2701 ccacatcacc gtcggcgtca gcgacggcag gaaggattaa cggccatggg tcagaaggtc
     2761 aatccgttag ggatacgcct cgggatcgtc cgcgactgga ccagccgctg gtacgccggc
     2821 aagaagcagt tcccggtgca cgtgcacacc gactttcgcg tgcgcgagtt cctccaggag
     2881 aagctgaagg aggcgtccgt
//
LOCUS       DATLKL010000733         2229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_42673, whole genome shotgun sequence.
ACCESSION   DATLKL010000733 DATLKL010000000
VERSION     DATLKL010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42673"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /gene="atpA"
                     /locus_tag="VM713_09930"
     CDS             complement(<1..1088)
                     /gene="atpA"
                     /locus_tag="VM713_09930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005351502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HVH84622.1"
                     /translation="MAIKASEISDLIKQRIENFKSVTEARNVGTIISVTDGITRIHGL
                     ADARYGEMLEFPGNTFGLALNLEQDSVGAVVLGEYQHLKEGDTVKTTGRILEVPVGPE
                     LLGRVVDALGAPLDGKGPVKAARTAPIERVAPGVIYRKSVSQPVQTGYKAIDSMVPIG
                     RGQRELIIGDRQTGKTALAVDTIINQKTSGIKCVYVAIGQKQSTIANVVRKLEEHGAM
                     GHTVVVAASASTPAAMQYIAAYSGCTMGEYFMDNGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVSYLHSRLLERSARVNEEYVTMVSKGAVKGKTGSLTGLPIIETQA
                     GDVTAFVPTNVISITDGQIFLETDLFNAG"
     gene            complement(1098..1634)
                     /locus_tag="VM713_09935"
     CDS             complement(1098..1634)
                     /locus_tag="VM713_09935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HVH84623.1"
                     /translation="MADRTTIARPYARAAFEEARALKRLEPWSQALHVGAAVVADPRV
                     AALLGNPHVTPEELAQLVTGIAGPKLGAEGANFVRTLAANHRLAVLPEIAALFDTLKD
                     AEQGVADVTVTSAAPLGTAQQKALSAALTRRLKRTVRLHCATDPALLGGAVVQSGDLV
                     IDGSLRTRLERIAYELTA"
     gene            complement(1643..2113)
                     /locus_tag="VM713_09940"
     CDS             complement(1643..2113)
                     /locus_tag="VM713_09940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004411.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HVH84624.1"
                     /translation="MNINLTLVVQMLVFALLIFGTMKWIWPPLLAAMEARERRIAEGL
                     AAAEKGEEALSEARGQAEIIVREARERANQIIDHAQHRANDLVEQAKGTAGAEGARLI
                     AAAHQQIELDTTRARESLRREVAGIAVGAAAKLLGREIDAKKHADLLDELASEV"
BASE COUNT          318 a          737 c          785 g          389 t
ORIGIN      
        1 ccggcgttga agaggtcggt ctcgaggaag atctgaccgt cggtgatcga gatgacgttg
       61 gtcggcacga acgccgtcac gtccccggcc tgcgtctcga tgatcggcag cccggtgagc
      121 gagccggtct tgcccttgac cgcacccttg ctgaccatgg tgacgtattc ctcgttgacg
      181 cgcgccgagc gctcgagcag ccgcgagtgc aggtaggaca cgtcgccggg gaaagcctcg
      241 cgccccgggg gacggcgcag caggagcgag atctgccggt aggcgaccgc ctgcttggag
      301 aggtcgtcat agatgatgag tgcatcctcg ccgttatcca tgaagtactc gcccatggtg
      361 cagccggagt aggcggcgat gtactgcatg gcggcgggcg tcgaggccga ggccgccacc
      421 accacggtat gccccatggc gccgtgctcc tcgagcttgc gcaccacgtt ggcgatggtc
      481 gactgcttct ggccgatcgc gacgtagacg cacttaatgc ccgaggtctt ctggttgatg
      541 atggtgtcga ccgcgagcgc ggtcttgccc gtctggcggt cgccgatgat gagctcgcgc
      601 tggccgcggc cgatcggcac catcgagtcg atggccttgt agccggtctg caccggctgg
      661 ctcacggact tcctgtagat gacgcccggg gccacacgct cgatcggcgc ggtgcgcgcc
      721 gccttcaccg ggcccttgcc gtccaagggc gcgccgagcg catcgaccac gcgcccgagg
      781 agctccggtc ccaccggcac ttcgaggatg cggccggtgg tcttgacggt atcgccttcc
      841 ttcaggtgct ggtactcgcc gagcaccacc gcgccgaccg agtcctgctc gaggttgagc
      901 gccaggccaa aggtgttgcc cggaaattcc agcatctcgc cgtagcgcgc atccgccagc
      961 ccgtggatgc gcgtgatgcc gtcggtgacc gagatgatgg tgccgacgtt gcgcgcctcg
     1021 gtgacggact tgaagttctc gatgcgttgc ttgatgagat cgctgatctc ggatgccttg
     1081 atggccatgt gggttcctca ggcggtcaat tcgtaggcga tgcgctcgag gcgcgtacgg
     1141 agcgagccgt cgatcaccag gtcgccgctc tgcaccacgg cgccgccgag cagggccggg
     1201 tcggtcgcgc aatgcaggcg caccgtgcgc ttgaggcgcc gggtgagcgc ggcggacagt
     1261 gccttctgct gggcggtgcc gagcggcgcg gcggaggtga cggtgacgtc cgcgactccc
     1321 tgctcggcgt ctttcagcgt gtcgaagagc gcggcgatct cgggcagcac cgcgaggcgg
     1381 tggttggcgg cgagcgtgcg cacgaagttg gcgccctctg cgccgagctt tggtccggcg
     1441 atgccggtga cgagctgcgc gagctcttcc ggcgtcacgt gcggattacc gaggagtgcc
     1501 gcgacgcgcg gatcggcgac cacagcggcg ccgacgtgca gcgcctgcga ccagggctcg
     1561 agccgcttaa gggcgcgcgc ctcctcgaac gcggcccgcg cgtacggtcg ggcgatggtg
     1621 gtcctgtccg ccatgtctct cttcagacct cggaggcgag ctcgtcgagg aggtcggcgt
     1681 gcttcttcgc gtcgatctcg cggccgagga gcttcgcggc cgcgccgacg gcgatgcccg
     1741 ccacctcgcg ccgcaggctc tcgcgcgccc gcgtggtgtc gagctcgatc tgctggtgtg
     1801 cggcggcgat caggcgcgcg ccttcggcgc cggcggtgcc cttggcctgc tccaccagat
     1861 cgttggcgcg atgctgcgcg tgatcgatga tctggttggc gcgctcgcgc gcctcgcgca
     1921 cgatgatctc agcctggccg cgtgcctcgc tcaaagcctc ctcgcccttc tccgcggcgg
     1981 ccagaccctc ggcgatcctg cgctcgcgcg cctccatggc cgcgagcagc ggcggccaga
     2041 tccatttcat cgtgccgaag atcagcagag cgaacacgag catctgcacg accagcgtga
     2101 gattgatgtt cacgacttaa gtctcctgcc tgggacttcg gagcagcggc tcagtgagcc
     2161 gacagcgcgc ccaggaacgg attcgcgaac gtgaagaaca gcgcgaagcc gatgccgatc
     2221 atggctacc
//
LOCUS       DATLKL010000734         1639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_166795, whole genome shotgun sequence.
ACCESSION   DATLKL010000734 DATLKL010000000
VERSION     DATLKL010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166795"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /gene="istB"
                     /locus_tag="VM713_09945"
     CDS             complement(<1..657)
                     /gene="istB"
                     /locus_tag="VM713_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HVH84625.1"
                     /translation="MERTEVLELMTQLKLYGMRAAYDEVMTAGIKRRHEPPRILGDLL
                     AAEIAEKRARSIKYQLTIAKLPLSKDVDDFDFTGTPVNQSLIRDLAGGAFLAEQRNAV
                     LVGGTGTGKSHLAIAIARACIRSGARGRFYTVVDLVNPLENEARANRQGRLAEYLTRM
                     DFVVLDELGYLPFAQTGGQLLFHLVSRLYERTSIIVTTNLAFGEWPSVFGDAKMTTAL
                     L"
     gene            complement(657..>1639)
                     /locus_tag="VM713_09950"
     CDS             complement(657..>1639)
                     /locus_tag="VM713_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012778788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVH84626.1"
                     /translation="EMVFDAHARAFEFFKGACTRGIYDNMKTAVEAVFVGKDRRFNRR
                     FLQMCGHYLVEPTACTPAAGWEKGQVENQVGNIRERLFAPRLQVASYDELNAWLLDRC
                     IAYAQANRHPELVDRTIWQVFEAERSQLVPVVSRFDGFRATQAAVSKTCLVRFDNNKY
                     SVAARAVGRPVEIQSYAERIVIRQDGAIVGEHVRSFGRNQTIYDPWHYVPVLARKPGA
                     LRNGAPFKDWLLPANLEGVRRRLKGSDDGDRQMVKILAAALSDGLAAVEAACSEALAG
                     GVHSADVVLNILARHRDPGPVAPILAPAALRLRQAPVADCARYDRLRRAG"
BASE COUNT          281 a          535 c          552 g          271 t
ORIGIN      
        1 gagcaatgcg gtggtcatct tggcgtcacc gaacacgctc ggccattcgc caaaggcgag
       61 attggtggtg acgatgatgg aggtgcgctc atagagccgg ctgaccagat ggaagagcag
      121 ctggccgccg gtttgcgcaa agggcaggta gccgagctcg tcgagcacga cgaaatccat
      181 gcgggtgaga tattcggcaa gccgaccttg ccggttggcg cgggcctcgt tctccagcgg
      241 gttaacgagg tcgaccacgg tatagaagcg gccgcgggcg ccgctgcgga tgcaggcccg
      301 cgcgatggca atcgccaggt ggctcttgcc ggtgccggtg ccaccgacca gcacggcgtt
      361 gcgctgctcg gcgagaaagg cgccgccggc caggtcgcgg atcaaggact ggttgaccgg
      421 cgtgccagtg aagtcaaagt cgtcgacatc cttgctcagc ggcaatttgg cgatggtcag
      481 ctggtacttg atcgagcgcg cccgcttctc ggcgatctcg gcggcaagca ggtcgccgag
      541 gatgcgcggc ggctcgtgcc ggcgcttgat gccggcggtc atcacctcat cgtaggcggc
      601 tcgcatgccg taaagcttga gctgggtcat cagctcgagg acctcggtgc gttccatcag
      661 ccggcccggc gcagacggtc gtagcgggcg caatcggcga ccggcgcctg ccgcaggcgc
      721 agtgccgccg gcgccagaat cggcgccact ggcccgggat cgcgatgacg cgccaggatg
      781 ttgaggacga catcggccga atggacgcca ccggctaggg cttcgctgca ggcggcttcg
      841 acagcagcca ggccgtcgct cagtgccgcc gccaggatct tgaccatctg acgatcgccg
      901 tcatccgagc ccttcagccg gcgccgcaca ccctccagat tggcgggcaa tagccagtct
      961 ttgaagggcg cgccattgcg cagcgcgccg ggctttctgg cgagcactgg tacgtaatgc
     1021 cagggatcgt agatcgtctg gttacggccg aacgagcgca catgctcgcc gacaatggcg
     1081 ccgtcctggc ggatgacaat gcgttcggcg taggactgga tctccaccgg ccgccccact
     1141 gcacgcgcgg cgaccgaata cttgttgttg tcaaagcgca ccaggcaggt cttcgatacc
     1201 gcggcctgcg tcgcgcggaa cccgtcgaag cggctgacaa ccggcacgag ttgcgagcgc
     1261 tcggcctcga acacctgcca gatcgtccga tcgaccaatt ccgggtgccg gttcgcctgg
     1321 gcgtaggcga tgcaccggtc gagcagccag gcgttcaact cgtcatagct cgccacctgc
     1381 aggcgcggcg cgaacaggcg ctcgcggatg ttgccgacct ggttctcgac ctgccccttc
     1441 tcccagcccg ccgccggcgt gcaggcggtc ggctcgacca ggtaatgccc gcacatctgc
     1501 aggaagcggc ggttgaaccg gcggtccttg ccgacgaaca ccgcctcgac cgccgtcttc
     1561 atgttgtcat agatgccgcg cgtgcaggca cccttgaaga actcaaaggc ccgggcatgc
     1621 gcgtcgaaca ccatctcct
//
LOCUS       DATLKL010000735         4062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_211780, whole genome shotgun sequence.
ACCESSION   DATLKL010000735 DATLKL010000000
VERSION     DATLKL010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211780"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VM713_09955"
     CDS             complement(<1..238)
                     /locus_tag="VM713_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84627.1"
                     /translation="MAGKLPVAAALPVLQGDAATRPGQNTWLSAIVRWLFIATSLAFL
                     AGLLIAPLVTVFTMALARGFESYLVSFADPDTQAA"
     gene            complement(238..1035)
                     /gene="cysT"
                     /locus_tag="VM713_09960"
     CDS             complement(238..1035)
                     /gene="cysT"
                     /locus_tag="VM713_09960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HVH84628.1"
                     /translation="MTFGFTTFFLSAIVLLPLAALVLAAASMHWSEFVAVVSSPRALG
                     SYRLSFGASALAATINLVFGFVIAWTLVRYEFPGRKLIDALIDMPFALPTAVSGIALT
                     TVFAQHGWIGRYLEPLGIKVAYTWLGVTGALTLISMPFAVRSMQPALLEVQHDLEEAA
                     ETLGAGRVYAFRRIILPTVLPALLTGFTLSFARAVGEYGSVIFIAGNLPMKTEITPLL
                     IVIHLEQFDYRGAAALGVVMLAMSFIVLLAINLLQTWNRRRQVRAAH"
     gene            complement(1090..2844)
                     /locus_tag="VM713_09965"
     CDS             complement(1090..2844)
                     /locus_tag="VM713_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVH84629.1"
                     /translation="MSITLEQVTKHYGGVPAVNDVSLDIGDGEFFVLLGPSGSGKSTL
                     LRAIAGLTGVDQGRIALHGRDVTTVAARKRGVGLVFQNYALFRHMTVADNIEFALRVR
                     RMPARHRLERRRELLRLVALEGMDDRLPVQLSGGQQQRVAVARALAHKPEVLLLDEPF
                     GALDAKIREELRRTIREVQRELGITTVLVTHDQGEAFAMADRIGIMNLGRLLEIGVPH
                     DLYSRPATRFVATFLGAANLFLARNGDAGIHVGAATLPAAEQAPQLREHEVVAVVRPE
                     EVAVAATPAELGPRFVAQGVVDEMVFAGALERLRVRLENGAGAPLLASAEAAATPYLE
                     VTRTPPEQRAFTVHPGQSVAIGVRRVHVLPTPLSSFTACAASAPAAETLARQPLLAEL
                     AARMKTRIGIRPEPQLGAPGAGPDRTEPFVGATVISCEPQAAQRAEWLLRHGANEVLV
                     LPPAAAVPQRVLIHWMDEAVRSATLDVSASVLRHVPAEAVYVRILPEGKRAARHAQGF
                     GALLDARSEAQAVHGLEMRTELRFGEVAEELARQLAEAPAQMLIVGVTDLTRFAERFS
                     ALLDGGRWPVLIVHRAAS"
     gene            2936..>4062
                     /locus_tag="VM713_09970"
     CDS             2936..>4062
                     /locus_tag="VM713_09970"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVH84630.1"
                     /translation="MRVPTSRVVVVGGGFAGLWAVRALRRAPVDITLIDSCNHHLFQP
                     LLYQVATAGLAAPSIAAPLRHILRRQRNVTVQLGTVAEIDKTSRTVVADGTRYAYDYL
                     ILATGVSHAYFGHDDWAGYAPGLKTLADALAMRARFLSAFERAESSQSQEEARAWLTF
                     VIVGGGPTGVELAGTLAEIARHTLKREFRRIDPRTAQVLLVEAGPRILPAFPADLSRS
                     AQGQLERLGVTVRTGSPVTSIGPEDVRIGEETVRTRTVIWAAGVAASSPGRALGVSLD
                     RTGRVLVADDLSVPGYPEIFVAGDLAAVSGAHGPVPGIAPAAKQMGSHAARSICARLA
                     GRGAAPFRYHHLALLATIGRRAAVVDLGRVRLTGTVAWWFW"
BASE COUNT          605 a         1411 c         1464 g          582 t
ORIGIN      
        1 tggcggcctg ggtgtcggga tcggcgaaac tcacgagata ggactcgaag ccgcgcgcga
       61 gcgccatggt gaatacggtc acgagcggcg cgatgagcag ccccgccagg aacgcgagcg
      121 atgtcgcgat gaacagccac cgtacgatgg cgctcagcca ggtgttctgc cctgggcggg
      181 tcgccgcatc gccttgcagc accggcagcg ccgccgcgac tggaagcttc ccggccatca
      241 gtgcgccgcc cgtacctgac gccgccggtt ccacgtttgc agcaggttga tggcgagcag
      301 cacgatgaac gacatggcga gcatcaccac gccgagcgcc gccgcgccgc ggtaatcgaa
      361 ctgctcgagg tggatgacga tgaggagcgg ggtgatctcg gtcttcatcg gcaggttgcc
      421 ggcgatgaag atcaccgagc cgtactcgcc gaccgcgcgc gcgaacgaca gggtgaagcc
      481 ggtgagcaat gccggcagca cggtcggcag gatgatgcgg cggaaggcat agacgcgccc
      541 ggcgccgagc gtctccgccg cctcctcgag atcgtgctgc acctcgagca gcgccggctg
      601 catcgagcgc accgcgaacg gcatgctgat gagggtcagg gcgccggtaa cgccgagcca
      661 ggtgtaggcc accttgatgc cgagcggctc gaggtagcgg ccgatccagc cgtgctgcgc
      721 aaacaccgtc gtgagcgcga tgccggagac cgccgtgggc agcgcgaacg gcatgtcgat
      781 gagcgcatcg atcagcttac ggccggggaa ctcgtagcgc accagcgtcc aggcgatgac
      841 gaagccgaac accaggttga tggtggccgc gagcgccgag gccccgaagg acaggcggta
      901 cgagccgagc gcgcgcgggc tcgagaccac cgcgacgaac tcgctccagt gcatcgaggc
      961 cgccgccagc accagcgctg cgagcggcag cagcacgatg gcgctcagga agaaggtcgt
     1021 gaaaccgaag gtcagcccga atcacggcag cacgctcggc ttgcggaagc tcatggctta
     1081 ccggctccct caggaggcgg cgcggtgcac gatcagcacc ggccagcggc cgccgtcgag
     1141 gagtgcgctg aagcgctcgg cgaagcgtgt gagatcggtg acgccgacga tcagcatctg
     1201 cgcgggcgcc tccgccagct gccgtgcgag ctcctcggcc acctcgccga agcgcagctc
     1261 ggtgcgcatc tcgagcccgt gcaccgcctg cgcttccgag cgcgcatcga gcagcgctcc
     1321 gaagccctgc gcgtggcgtg cggcgcgctt gccctcgggg agaatccgca cgtacaccgc
     1381 ctccgcaggc acgtgccgca gcacgctcgc cgatacgtcg agcgtcgcgc tgcgcaccgc
     1441 ttcgtccatc cagtggatca gtacccgttg cggaaccgcg gcggccgggg gcagcaccag
     1501 cacctcgttt gccccgtgcc tgagcagcca ctcggcgcgc tgcgcggcct gcggctcgca
     1561 gctgatcacg gtggcgccca cgaagggctc ggtgcggtcc ggtcccgccc ccggcgcgcc
     1621 gagctgcggc tcaggccgta tgccgatgcg ggtcttcatg cgtgcggcca gctcggcgag
     1681 cagcggctgg cgcgcgagcg tctcggcggc gggtgcgctc gcggcgcacg cggtaaagct
     1741 cgacagcggc gtcggcagca cgtgcacgcg gcgtacgccg atggcgacgc tctgcccggg
     1801 atgcaccgtg aaggcacgct gctccggcgg cgtgcgcgtc acctcgaggt agggggttgc
     1861 agccgcctcc gcggatgcga gcagcggcgc cccggcgccg ttctcgagcc gcacgcgcaa
     1921 ccgctcgagc gctccggcaa ataccatctc gtcgaccacg ccctgcgcga cgaagcgcgg
     1981 gccgagctcg gctggagtgg cggccaccgc gacttcttcg gggcgcacca ccgcgaccac
     2041 ttcgtgctcg cgcagctgcg gcgcctgctc ggcggcggga agggtggcgg cgccgacgtg
     2101 aatgcccgcg tcgccgttgc gcgcgaggaa gaggttcgcg gcgccgagaa aggtggcgac
     2161 gaagcgcgtc gcggggcgcg agtagagatc gtgtggcacg ccgatctcga gcagccgccc
     2221 gagattcatg atcccgatgc ggtcggccat cgcgaacgct tctccctggt cgtgcgtgac
     2281 cagcaccgtc gtgatgccga gctcacgctg cacctcgcgg atggtgcggc gcagctcctc
     2341 gcgaatcttg gcgtcgagcg cgccgaaggg ctcatcaagc agcagcacct cgggcttgtg
     2401 ggccaacgcc cgcgccaccg cgacccgctg ctgctggccg cccgagagct gcaccggcag
     2461 ccggtcgtcc atgccttcca gcgccaccag gcgcagcagc tcccggcggc gctcgaggcg
     2521 atggcgtgcg ggcatgcggc gcacccgcag cgcgaactcg atgttgtccg ccaccgtcat
     2581 gtgacggaac agcgcgtagt tctggaacac gaggccgacc ccgcgcttgc gcgcggcgac
     2641 ggtggtgacg tcgcggccat gcagcgcgat gcgcccctgg tcgaccccgg tgagccccgc
     2701 gatggcgcgc agcagcgtgc tcttgccgct gccgctcggg ccgagcagca cgaagaattc
     2761 cccgtcaccg atgtcgagcg acacgtcgtt gacggccggg acgccgccgt agtgcttcgt
     2821 gacctgctcg agggtgatgg acatggcggg aatgctacct gagcggaggc tcggcccgtc
     2881 agccaggctg atcggcctgt tcggcggccg tgcttcgtga tatttcagcg taacggtgcg
     2941 ggtaccaact tcgcgggtgg tggtcgtcgg cggcgggttt gccggactgt gggcagtgcg
     3001 ggcgctgcgc cgcgccccgg tcgacattac gctcatcgac tcctgcaacc accatctgtt
     3061 tcagcccctg ctctaccagg tcgcgaccgc cgggctcgcc gcgccgtcga tcgccgcgcc
     3121 gctgcggcat atcctgcggc ggcagcgcaa tgtcacggtc cagctcggta ccgtggcgga
     3181 gattgacaag acctcgcgaa cggtggtcgc cgatggcacg cggtacgctt acgactatct
     3241 gattctcgcg acgggcgtct cgcatgcgta cttcgggcac gacgactggg ccggctacgc
     3301 gcccggcctg aagacactgg cggacgcctt ggcgatgcga gcgcgcttcc tcagcgcctt
     3361 cgagcgcgcc gagagcagcc aatctcagga ggaggcccgc gcctggttga ctttcgtcat
     3421 cgtcggcggc ggaccgaccg gcgtcgagct ggctgggacg ctcgccgaga ttgcccggca
     3481 tacgctcaag cgcgagttcc gccgcatcga cccacgcacg gcgcaggtgc tgctggtgga
     3541 ggccggccca cgcatcctgc cggcgtttcc tgcggatctt tcgcgcagcg cccaggggca
     3601 gctcgagcgg ctcggcgtca cggtgcgcac cggctcgccg gtcacctcga tcgggccgga
     3661 ggacgtgcgc atcggtgagg agacggtgcg cacgcgcacg gtcatctggg cggccggggt
     3721 cgcggcctca tcgcccggtc gggcgctcgg ggtgagcctc gatcgcaccg ggcgcgtgct
     3781 cgtcgcggac gatctcagcg tcccgggcta ccccgagata tttgtggcgg gggatctcgc
     3841 cgcggtcagc ggagcgcacg gcccggttcc gggcatcgcg cccgcggcca agcagatggg
     3901 cagccatgcc gcccggtcga tctgtgcgcg tctcgcgggg cggggcgcag cgcccttccg
     3961 ctaccaccac ctggcactgc tcgccaccat cggccgcagg gccgcggtgg tcgacttagg
     4021 ccgcgtgagg ctcacgggca cggtggcctg gtggttctgg tt
//
LOCUS       DATLKL010000736         2739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_133059, whole genome shotgun sequence.
ACCESSION   DATLKL010000736 DATLKL010000000
VERSION     DATLKL010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133059"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VM713_09975"
     CDS             <1..510
                     /locus_tag="VM713_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HVH84631.1"
                     /translation="MVPRRAARSEPRSDENAERAADPTAARAAAVALLARRDLPSGEL
                     RERLSGRGFEPEASAAALASLTAEGALNDERYAHNYVAYHAGRGRGPIRIGADLRARG
                     LPQELIEVALASGPDWRASAAAARARRFGKLAPASWREKARQARFLQYRGFSADHIRA
                     ATGADPDTD"
     gene            535..>2739
                     /gene="alaS"
                     /locus_tag="VM713_09980"
     CDS             535..>2739
                     /gene="alaS"
                     /locus_tag="VM713_09980"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HVH84632.1"
                     /translation="MSAADVRAAFLEFFRGHAHAIVPSSSLVPANDPTLLFTNAGMVQ
                     FKDVFLGRDTRDYLRAASSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RQAIRFAWDFLIFNLGLDPARLWCTVYTDDDEAADIWLKEVGVSPRRFARLGEASNFW
                     AMGDTGPCGPCSEIFYDHGPEVAGGPPGSAEEEGDRYVEIWNLVFMQYDRGADGKLTP
                     LPKPSVDTGMGLERISAVMQGVHSNYDIDLFKNLIRAAGKLAGTSVLTSSSLRVIADH
                     IRASTFLVVDGVLPSNEGRGYVLRRIIRRAIRHGYKLGIHEPFFYKLVPVLAQEMGAA
                     YPELLSRRSVTERVLRQEEERFAQTLANGMVLLESAIRNLRGEQVIDGETVFKLYDTY
                     GFPADLTADVARERGLTIDQAGFETAMGQQRRRSQESSKFSTDQSAGVTVDAETVFRG
                     YEELRCAGEVVALLKDGASVPALAAGDKGEVVLDRTPFYAESGGQVGDAGELTGPGTR
                     FAVADTQKRGAAHVHLGEVAEGTIRVGDTLEARVDARRRQATVLNHTATHLLHAALRA
                     VLGTHVQQKGSLVAPDRLRFDFAHYQAVTPEELLEIERLVNAEIRRNSPGEVRVMDYD
                     AAVGEGAIALFGEKYDKQVRVLKVGDFSMELCGGTHVQRAGDIGLFKIVSESGVASGV
                     RRIEALTGEAALALVEQNDALLKDLAGLVRGSREELGDKVREQLERVKQLERE"
BASE COUNT          429 a          909 c          981 g          420 t
ORIGIN      
        1 atggtccccc gccgagcagc aagatccgag ccgcgctcag atgaaaacgc ggagcgcgcc
       61 gccgacccta cggcggcccg ggcggcggcg gtcgccttgc ttgcgcggcg cgacctgccg
      121 agcggtgagc tgcgcgagcg cctgagcggg cgcgggttcg agcccgaggc gtcggcggcg
      181 gcactggctt cgctcaccgc cgaaggcgcg ctcaacgatg agcgctatgc gcacaactat
      241 gttgcctacc acgcgggccg cggccgcggc ccgatccgca tcggcgcgga tctgcgcgcg
      301 cgcggcctgc cgcaggagct catcgaggtg gcgctcgcga gcggtccgga ctggcgcgcc
      361 tcggcggcag cggcccgggc acgccgcttc ggcaagctcg ccccggcgag ctggcgcgag
      421 aaggcgcggc aagcacgctt tttgcagtat cgtggctttt cagcagatca tatccgcgcg
      481 gctaccggag ccgatcccga cacggattga agcatcttgg gtaaaccgac ctacatgagc
      541 gcagcggacg tgcgcgcggc gtttctcgag ttcttccgcg ggcacgctca cgcgatcgtg
      601 ccctcgagct cgctcgtgcc cgcgaacgac ccgacgctgc tgttcaccaa cgccggcatg
      661 gtgcagttca aggacgtgtt cctggggcgc gacacgcgcg actacctgcg cgcggcgagc
      721 agccagcggt gcgtacgcgc gggcggcaag cacaacgact tggagaacgt cggctacacg
      781 gcccgccacc atacgttctt cgagatgctc ggcaacttct cattcggcga ctacttcaag
      841 cgccaggcga tccgcttcgc ctgggatttc ctgatcttca acctggggct ggatccggcg
      901 cggctctggt gcacggtgta cacggacgat gatgaggccg cggacatctg gctgaaggag
      961 gtcggcgtga gcccgcggcg gtttgcgcgc ctcggcgaag cgtccaactt ctgggcgatg
     1021 ggagacaccg gtccctgcgg cccatgcagc gagatcttct acgaccacgg cccggaggtc
     1081 gcgggcggcc cgccaggctc cgcggaggag gagggcgatc gctacgtcga gatctggaac
     1141 ctggtcttca tgcagtacga ccggggtgcg gacggcaagc tgaccccgct gccgaagccc
     1201 tcggtcgaca ccggcatggg cctcgagcgc atttccgccg tgatgcaggg cgtgcattcc
     1261 aactacgaca tcgacctgtt caaaaatctg atccgcgccg ccggcaagct cgccggcacg
     1321 tcggttctca cctcgagctc gctgcgtgtg atcgccgatc acatccgcgc cagcaccttt
     1381 ctggtcgtcg acggcgtgct gccctccaac gagggccgcg gctacgtgtt gcgccggatc
     1441 atccgccgcg cgatccgcca cggctataaa ctcggcatcc acgagccgtt cttctacaag
     1501 ctggtgccgg tgctggcgca ggagatgggc gccgcctatc cggaactgct ctcgcgccgc
     1561 tcggttaccg agcgtgtgct gcgccaggag gaggagcgct tcgcccagac gctcgccaac
     1621 ggcatggtgc tgctcgagag cgcgatccgg aatctgcgcg gcgagcaggt gatcgacggc
     1681 gagaccgtgt tcaagttgta cgacacctac ggctttccgg ccgacctgac cgcggacgtc
     1741 gcgcgcgagc gcgggctcac catcgatcag gcgggcttcg agacggccat ggggcagcag
     1801 cggcgccgct cgcaggaatc gagcaagttc agcaccgacc agagcgccgg ggtcaccgtg
     1861 gatgccgaga ctgtgttccg cggatatgag gagctcaggt gcgccggcga ggtcgtggcg
     1921 cttctgaagg acggcgcctc ggtgccggct ctggccgccg gcgacaaggg cgaggtggtg
     1981 ctcgatcgca cgccgttcta tgccgaatcc ggcggccagg tgggtgatgc gggagagctg
     2041 acggggcctg gcacgcgctt tgcggtcgct gatacccaga aacgcggcgc cgctcacgtg
     2101 cacctcgggg aggtggcgga gggcacgatc cgcgtcggcg acacgctcga ggcgcgggtg
     2161 gatgccaggc ggcgccaggc cacggtcctc aatcacacgg cgacgcacct gctgcacgcg
     2221 gcgctgcggg cggtgctcgg cacccacgtg cagcagaagg gctcgctggt ggcgcccgat
     2281 cggctgcgct tcgactttgc gcactaccag gccgtgacgc ctgaggagct tctcgagatc
     2341 gagcggctcg tgaatgcgga gatccgccgt aactccccgg gcgaggtccg cgtcatggac
     2401 tatgacgccg cggtgggcga aggcgcgatc gcgctgttcg gcgagaagta cgacaagcag
     2461 gtgcgggtgc tgaaggtcgg cgatttctcg atggagctgt gcggcggcac ccacgtgcag
     2521 cgcgcgggcg acatcggcct cttcaagatc gtgagcgaga gcggcgtcgc ctccggcgtg
     2581 cgccgcatcg aggccttgac gggcgaggcg gccctcgctc tcgtggagca gaacgacgcg
     2641 ctgctcaagg acctcgccgg cctcgtgcgc ggctcgcgcg aggagctcgg cgacaaggtg
     2701 cgcgagcagc tcgagcgggt gaagcagctc gagcgcgag
//
LOCUS       DATLKL010000737         2515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_42725, whole genome shotgun sequence.
ACCESSION   DATLKL010000737 DATLKL010000000
VERSION     DATLKL010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42725"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(419..553)
                     /locus_tag="VM713_09985"
     CDS             complement(419..553)
                     /locus_tag="VM713_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84633.1"
                     /translation="MLLRIDAEAGHGIGSTKTQTDALTADWVAFVFWRSGLLGWQPAP
                     "
     gene            complement(583..1440)
                     /gene="kynA"
                     /locus_tag="VM713_09990"
     CDS             complement(583..1440)
                     /gene="kynA"
                     /locus_tag="VM713_09990"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HVH84634.1"
                     /translation="MTTHRYDKSERVELADEAVHWDLGSSLSYGEYLHLEQLLSAQHP
                     LTGEHNEMLFVIVHQVSELWMRLMLHELTAALECVRRDDLDPALKMLARISRSQNQLI
                     AVWDVLSTMTPFEYTAFRNALGRSSGFQSVQYRMLEFLFGNKNAEMLAVHKRDPAAYA
                     ALERALAEPPLYDEVLRLLSRRGYGIPEDYLSRDFREPYVANKQVAGAWLGVYHNADT
                     GWDLYELAERLVDLDHNFQLWRCHHLKTVERIIGYKPGTGGTGGVSYLAKALELKFFP
                     ELWQIRTSM"
     gene            1552..2439
                     /locus_tag="VM713_09995"
     CDS             1552..2439
                     /locus_tag="VM713_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVH84635.1"
                     /translation="MSRPLLEVLSLVKQYPATTAVDGVSFHVDEGICFGLLGPNGAGK
                     TTTVEIMEGILPPTSGEVRYHGEPLGPRFREEAGILFQKTALQDFLTVRQCIALFRGL
                     YTHGLEVDEVLRICALEKLAGREARKLSGGQQQRLLLAIALVNDPAVLFLDEPTTGLD
                     PQARRNFWQLVESIKARRKTILLTTHYMEEAELLCDEIAIMDRGRIVAQGPPRRLLRE
                     HFAEVLLELPRQDFPEGARTLPLKLMDASDRVEITTHDLEETLRTLLAAHVPLTNLRV
                     RPPNLEDLFLELTGKELRP"
BASE COUNT          439 a          861 c          804 g          411 t
ORIGIN      
        1 tggcgtccac acctgactcg acacgagata taacgtcact ctgcatcgac agcggccgca
       61 gcatttgtca cgcgcgtcgc gtataacaac ctgcggcccc tcgtcctcat cgatgagcct
      121 tcacgagggc attgagcgag ccagcccagt cagcggcttc gctttggtcc ccagcgaatc
      181 agtgcaaaga tcgtctcgtg aaggaacaaa ccacgcaccc cggacaatcc ccggcagggc
      241 gtgcaaccga gagcggaatc gaagtccagg aagaggagtg cggaacggtc accgcgcagc
      301 aggtgtacaa gttgcgttgc gactgtggcc gctcatggtt cgagctcgag ctgccgacgt
      361 tcgtaaagtg tccggcctgc aacaggctcg ggcacgttac tagatagagc tagcgtcgct
      421 atggggcggg ctgccagccg agcagtccgc tgcgccagaa cacgaacgcg acccagtctg
      481 ccgtgagcgc atcggtctgc gtcttggtcg agccgatgcc atggccggct tcggcgtcga
      541 tgcgcaggag caccgggttc tgcgcttgct cggctgtctg agctacatgg acgtgcggat
      601 ctgccacagc tccggaaaga acttgagctc gagcgccttg gcgagatagg acacgccgcc
      661 cgtgccgccg gttcccggct tgtagccgat gatgcgctcc accgtcttca ggtgatggca
      721 gcgccataac tggaagttgt gatcgaggtc cacgagccgc tcggcgagct cgtagagatc
      781 ccagcccgtg tcggcgttgt ggtacacccc gagccaggcg ccggcgacct gcttgttcgc
      841 cacgtagggc tcgcggaagt cgcgtgacag gtaatcctcc ggaatcccat agccgcgccg
      901 actgaggagc cgcagtacct cgtcgtagag cgggggttcg gcgagcgcgc gctccagggc
      961 cgcgtaagcc gcgggatcgc gcttgtgcac ggcgagcatc tcggcgttct tgttgccgaa
     1021 gaggaattcc agcatgcggt actgcaccga ctggaagccc gaggagcgcc cgagcgcgtt
     1081 gcgaaacgcc gtgtactcga atggcgtcat ggtcgagagc acgtcccaca ccgcgatcag
     1141 ctggttctgc gagcgcgaga tgcgcgccag catcttgagc gccgggtcga gatcgtcgcg
     1201 ccgcacgcac tcgagcgcgg cggtcagctc gtggagcatg aggcgcatcc acagctccga
     1261 cacctggtgc acgatgacga acagcatctc gttgtgctca cccgtcagcg ggtgctgcgc
     1321 gctcagcagc tgctcgagat gcaggtactc tccgtaggag agcgagctgc cgagatccca
     1381 gtgcaccgcc tcgtcggcga gctcgacgcg ctcagacttg tcatagcggt gggtagtcat
     1441 gcggctgatg gtacacgtca cgcaccgcgc ggcaataccg cagcccgcgc accatctgtc
     1501 atacgccgcc tcaagacgcc ccggccccgg atccgctagg atcgcgcgac cgtgagccga
     1561 cccctcctcg aagtcctgag tctcgtcaag cagtaccccg ccaccacggc cgtggacggc
     1621 gtcagcttcc acgtggacga gggaatctgc ttcgggctcc tcggtcccaa cggcgccggc
     1681 aagaccacca cggtcgagat catggagggc atcctgccgc cgacctcggg cgaggtgcgt
     1741 taccacggcg agcccctcgg gccgcgcttt cgcgaggaag ccggcatcct cttccagaag
     1801 accgcgctgc aggacttcct caccgtgcgc cagtgcatcg cgctgtttcg cggcctctat
     1861 acccacggtc tcgaggtaga cgaggtgctg cgcatctgcg cgctcgagaa gctcgccggg
     1921 cgcgaggcgc gcaagctctc cggcgggcag cagcagcggc tgctgctcgc catcgccctg
     1981 gtgaacgacc cggcggtgct gttcctcgac gagccgacca ccgggctcga tccgcaggcg
     2041 cggcgcaatt tctggcagct ggtcgagtcg atcaaggcgc gccgcaagac catcctcctc
     2101 acgacccact acatggagga ggcggagctc ctgtgcgacg agatcgcgat catggaccgc
     2161 ggccgcatcg tcgcccaggg tccgccgcgg cggctgctgc gcgagcactt cgccgaggtg
     2221 ctgctcgagc tgccgcgcca ggactttccg gagggcgccc gcaccctgcc cctcaaactc
     2281 atggacgcca gcgaccgcgt cgagatcacc acccacgatc tcgaggagac gctgcgcacg
     2341 ctgctcgccg cgcacgtgcc actcaccaac ctgcgcgtgc ggccgccgaa cctcgaggac
     2401 ctgttcctcg agctcaccgg caaggagctg cggccgtgat ccgccggctg ctctcggtat
     2461 tccacgcacg caacctcgag ttcgtccgcg accgcggcac gctgatattc acgcc
//
LOCUS       DATLKL010000738         3411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_189476, whole genome shotgun sequence.
ACCESSION   DATLKL010000738 DATLKL010000000
VERSION     DATLKL010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189476"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VM713_10000"
     CDS             <1..359
                     /locus_tag="VM713_10000"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692099.1"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer composed of two alpha (AccA)
                     and two beta (AccD) subunits; one of the two catalytic
                     subunits that can form the acetyl CoA carboxylase enzyme
                     together with a carrier protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HVH84636.1"
                     /translation="GALAIGVCDRLLMLQYSTYSVISPEGCASILWKSQDKKEVAAES
                     LNLTADRLQKLGLVDEVIEEPLGGAHRDPATTAARLKAALIRELDELDALPRDQLRAA
                     RAAKIASFGVFSENAA"
     gene            375..1760
                     /gene="tilS"
                     /locus_tag="VM713_10005"
     CDS             375..1760
                     /gene="tilS"
                     /locus_tag="VM713_10005"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HVH84637.1"
                     /translation="MGGKPEEFGASWLGRRLGELLPDFPDRALCVAFSGGPDSTALLA
                     ALAQLKRRPRALRAVHVDHGLHPDSGSWAAHAVEVAQALSVPCVVLAAEVSRAHGESP
                     EARARAARYRLLAEALTEGEVLLTAHHEDDQLETVLLMLLRGAGVAGLAAMPEVAPFA
                     RGLLARPLLTRPRAELVAWVRAQGLACLEDPSNLDERFDRNFLRLRVLPVIRERWPGA
                     PATVSRAARHAAEAQRLLETLARADIRAARNGESLAASALRTLPPDRRRNALRFWISS
                     RGLTLPPTARLKEIAGPLLAARADAHPSVAWPGARVELHSGLLTLRATGRAPATMAGG
                     EIPWRWRAQPVCALPGGLGRLELKADAHGPVNLAALPARLTIRWRRGGERLTPAPGRP
                     RRALKSLLRESRLLPAQRARLPLVYGGTQLLAVADLWLDESVQAHPGTRRRARLVWSR
                     AAAVESAPEMC"
     gene            1831..>3411
                     /locus_tag="VM713_10010"
     CDS             1831..>3411
                     /locus_tag="VM713_10010"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HVH84638.1"
                     /translation="MTRFVFVTGGVVSSLGKGIASASLGAILEARGLKIAMVKLDPYI
                     NVDPGTMSPFQHGEVFVTSDGTETDLDLGHYERFVRTTTGRNSNFTTGRIYERVIAKE
                     RRGDYLGATVQVIPHITDEIKRCIKLGADGADVCMVEIGGTVGDIESLPFLEAIRQLG
                     VELGRSNCVYMHLTLVPVVGGSHEVKTKPTQHSVKELRGIGIQPDVLLCRCKHPLPEE
                     QRRKIALFTNVEERAVISAVDADDIYRIPILLHEQALDDIVVDKLRLTVPPTDLAEWR
                     HVVSAKSNPEGQVDIAMVGKYVQIRDSYLSLHEALMHGGLKSRTRVNIHYVESTDIEQ
                     HGTHVLKGMDAILVPGGFGERGIEGKIQAVRFAREQRVPYLGICLGMQVAIVEFGRHM
                     LGLADANSTEFNRASPHPVIALISEWQDRAHGAQTRTETSEKGGTMRLGAQEVLLGAG
                     TRVRELYGRDVIMERHRHRYEFNNNYLERYRQAGLRFSGFSRDNLVEVIELASHPWFV
                     ATQFHPEFTSTPRDGHPLF"
BASE COUNT          502 a         1217 c         1169 g          523 t
ORIGIN      
        1 ggggcgcgct ggcgatcggt gtgtgcgacc gcctcctcat gcttcagtac agcacctact
       61 cggtgatttc cccggagggc tgcgcctcga ttctctggaa gagccaggac aagaaggaag
      121 tggcagcgga gtcgctcaac ctgacggccg atcggctgca gaagctcggg ctcgtcgatg
      181 aggtgatcga ggagcccctc ggcggcgcgc accgcgaccc cgccacaacc gccgcgcggc
      241 tcaaggccgc cctgatccgc gagctcgacg agctcgacgc gctgccgcgc gatcagctgc
      301 gggccgcgcg cgccgccaag atcgcctcct tcggcgtgtt ctcagaaaac gcggcctgat
      361 cagccgcagc aaccatgggc ggaaagccgg aggaattcgg cgccagctgg ctcggccggc
      421 ggctcggtga gctgctgccg gactttcccg accgtgccct gtgcgtcgct ttcagcggcg
      481 gaccggactc cacggcgctg ctcgcggcgc tcgcgcagct gaagcgccgg ccgcgcgcgc
      541 tgcgtgccgt gcacgtcgat cacgggctcc accccgattc cggttcctgg gccgcccacg
      601 ccgtcgaggt cgcgcaggct ttgagcgtac cgtgcgtggt gctcgcggcc gaggtgagcc
      661 gcgcgcacgg cgagtcgccg gaagcccggg cgcgcgcggc gcgctaccgg ctgctggcag
      721 aggcgctcac cgaaggcgag gtgctgctca ccgctcacca cgaggacgac cagctcgaga
      781 ccgtgctgct catgctgctg cgcggtgccg gagtggcggg tcttgccgcg atgcctgaag
      841 tcgccccgtt cgcgcgcggt ttgctggcgc gtccgctgct cacgcgcccg cgcgccgagc
      901 tggttgcctg ggtgcgagcg cagggcctcg cctgcctcga ggatccgagc aacctggatg
      961 agcgcttcga tcgcaacttc ctgcggctga gggtgctgcc ggtgatccgg gagcgctggc
     1021 ccggcgcgcc ggccacggtg agccgggcgg cgcggcatgc cgccgaagcg cagcgcctgc
     1081 tcgagacctt ggcgcgcgcc gacatcagag ccgcccgcaa cggtgagtcg ctcgcggcga
     1141 gcgcgctgcg gacgcttccg ccggatcgcc ggcgcaacgc gctgcgattc tggatcagct
     1201 cgcgcggcct gacgctgccg ccgaccgccc gtctcaagga gatcgcgggg cccctgctgg
     1261 ccgcccgcgc cgatgcgcac ccgtccgtcg cctggccggg tgcgcgggtt gagctccaca
     1321 gcgggctcct cacgctgcgc gccaccgggc gcgcgcccgc cacgatggcc ggcggcgaaa
     1381 tcccctggcg ctggcgcgcg cagcccgtct gcgctctgcc cggcggcctc gggcggctcg
     1441 agctcaaggc cgacgcccac gggccggtga acctcgccgc actgccggcc cggctcacca
     1501 tccgctggcg ccggggcggg gagcgcctca ctccggcccc cggtcggccg cggcgcgcgc
     1561 tcaagagcct gctgcgcgaa tcgcggctgc tcccggcaca gcgcgcacgg ctgccgctcg
     1621 tctatggcgg cacgcagctg ctggcggtcg cggatctgtg gctcgatgag tcggtacagg
     1681 cgcatccggg gacgcgccgc cgcgcgcgcc tcgtgtggag ccgtgccgca gccgtcgagt
     1741 cggcgcctga gatgtgctaa tctgctggcc cgtcgtcgat ctgccctttc tgcggcagcg
     1801 acctcacgcg acgggtaatc ctgcgagact atgacgcgtt tcgtattcgt caccggcggt
     1861 gtcgtgtcct ccctgggcaa gggcattgcc tccgcctcgc tcggcgcgat tctcgaggcc
     1921 cggggcctca agatcgccat ggtcaagctc gatccctaca tcaacgttga tccgggcacc
     1981 atgagcccgt ttcagcacgg cgaggtgttc gtcacctcgg atggaaccga gaccgatctc
     2041 gacctcgggc actacgaacg cttcgtgcgc actaccacgg ggcgtaacag caacttcacc
     2101 accgggcgca tctacgagcg agtgatagcc aaggagcggc gcggcgacta tctgggagcg
     2161 accgtccagg tcatcccgca catcaccgac gagatcaagc gctgcatcaa gctcggcgcc
     2221 gacggcgccg acgtctgcat ggtcgagatc ggcggcacgg tgggtgatat cgaatcgctg
     2281 ccgttcctcg aggccatccg ccagctcggg gtcgagctcg gccgcagcaa ctgtgtctat
     2341 atgcacctga cgctggtgcc ggtggtgggc ggctcgcacg aggtgaagac caagccgacc
     2401 cagcactcgg tcaaggagct gcgcggtatc ggtatccagc cggacgtgct cctgtgccgc
     2461 tgcaagcatc ccctgcccga ggagcagcgg cgcaagatcg ccctctttac caatgtcgag
     2521 gagcgcgcgg tgatttccgc ggtggacgcc gacgatatct accgcattcc gatcctgctg
     2581 cacgagcagg cgctcgacga catcgtggtc gacaagctgc gcctcaccgt gccgccgacc
     2641 gatctcgccg agtggcgcca cgtcgtgagc gccaagtcga atcccgaggg gcaggtcgac
     2701 atcgcgatgg tcggcaagta cgtgcagatc cgcgattcct acctctcgct ccatgaggcc
     2761 ctcatgcacg ggggtctcaa gagccgcacg cgcgtcaata tccactacgt cgagtcgacc
     2821 gacatcgagc agcacggcac ccacgtgctg aaggggatgg acgcgatcct ggtgcccggc
     2881 ggcttcggcg agcggggcat cgagggcaag atccaggcgg tgcgcttcgc gcgcgagcag
     2941 cgggtgcctt atcttgggat ctgcctgggg atgcaggtgg cgatcgtgga gttcggccgg
     3001 cacatgctcg gcctcgccga cgccaacagc accgagttca accgcgcctc cccgcatccc
     3061 gtgatcgcgc tcatttccga atggcaggac cgggcgcacg gcgcgcagac gcgcaccgag
     3121 acctccgaga agggcggcac catgcgcctc ggcgcgcagg aggtgctgct cggggcgggg
     3181 acgcgcgtcc gcgagctcta cggccgcgac gtcatcatgg agcgtcatcg gcatcgctac
     3241 gagttcaaca acaactacct cgaacgctac cgccaggccg gactgcgctt ctcgggcttc
     3301 tcgcgcgaca acctggtgga ggtgatcgag ctagccagtc acccctggtt cgtggcgacg
     3361 cagttccacc ccgaattcac ctccaccccg cgcgacgggc acccgctgtt c
//
LOCUS       DATLKL010000739         3441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_42781, whole genome shotgun sequence.
ACCESSION   DATLKL010000739 DATLKL010000000
VERSION     DATLKL010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42781"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VM713_10015"
     CDS             complement(<1..1005)
                     /locus_tag="VM713_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVH84639.1"
                     /translation="MTVPRVPAPLLAVAALVLGLAPPAVAQTQPAGIIVLKAAHIFDA
                     RDGALRDGGVVTVQGERIVSVGAANAPAGARVIDLGDATLLPGFIDAHTHVTMQFEKN
                     YYLMRYHELMRFPAEQVLYGALYAKRTLEAGFTTIRNVGAADFEDISLRNAINAGITE
                     GPRMLVSAHGIGSPGGHFDQLSYPPDRVHPEGPIQGICSGPEECREAVRYQMKWGADV
                     IKIAASGGVLSESDPVDVPQLTLEEMKAIVSEAHAWRRKVAAHCHGDAAARLAIEAGV
                     DSIEHGSFLTEDTLKLMKARGVVLVPTRITVEWVLKEVEGYPPNIAAKARAAGAAHTG
                     M"
     gene            complement(1026..1994)
                     /locus_tag="VM713_10020"
     CDS             complement(1026..1994)
                     /locus_tag="VM713_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HVH84640.1"
                     /translation="MTLAEIRALVQADLNSVDEVIRARLSSAVPLVDRVADHIIAGGG
                     KRLRPLLVVLAGRACGQGGGAHIEAAAFIEFIHTATLLHDDVVDGSSLRRGRDTANEV
                     FGNQASVLVGDFVYSRAFQMMAALASQPVMEIMADATNVIAEGEVLQLMNAHDPDTTE
                     QRYLEVIYRKTARLFQAGAEVAAVLASSAAERRAALAAYGRHLGTAYQLVDDVLDYCS
                     TPAERGKNLGDDLAEGKPTLPLIHALRHGSETQRAAIRAAIQSGGRGHLGDVVDAIES
                     TGGLDYAARLARTEADAALRTLALLPDSSYKTGLAALANFAVEHTT"
     gene            2158..2472
                     /gene="rplU"
                     /locus_tag="VM713_10025"
     CDS             2158..2472
                     /gene="rplU"
                     /locus_tag="VM713_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HVH84641.1"
                     /translation="MYAVIATGGKQYRVTKDGVLRVEKLDAEPGATVEFGEVLLVGEG
                     ADVKVGKPTLKGGKVLATVVRHGKGAKVSIVKFRRRKHYLRQKNHRQLYTEVKVTDIR
                     AG"
     gene            2477..2746
                     /gene="rpmA"
                     /locus_tag="VM713_10030"
     CDS             2477..2746
                     /gene="rpmA"
                     /locus_tag="VM713_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019411882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HVH84642.1"
                     /translation="MAHKKAGGSTKNGRDSHSKRLGVKRFGGEQVLAGNIIVRQRGTQ
                     VRPGANVGVGTDHTLFAKVTGKVEFRKKGAEQRLYVSVLPEPQKA"
     gene            2844..>3441
                     /gene="obgE"
                     /locus_tag="VM713_10035"
     CDS             2844..>3441
                     /gene="obgE"
                     /locus_tag="VM713_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HVH84643.1"
                     /translation="MKFVDEARARVQAGSGGRGSASFRREKFVPFGGPDGGDGGHGGS
                     VYLRAATGINTLADFRIERTFKAAHGEPGGSNDCSGRGGADLHVPVPVGTVVRDAETH
                     EQLGDLTHDGEELLVARGGKGGWGNQRFKSSTNRAPRQFGPGLPGERRSLELELKVIA
                     DVGLLGLPNAGKSTLIRAVSAARPKVADYPFTTLHPNLG"
BASE COUNT          546 a         1105 c         1244 g          546 t
ORIGIN      
        1 catgccggta tgcgcggcgc ccgcggcgcg cgctttggcg gcgatgttcg gcgggtagcc
       61 ctcgacttcc ttcagcaccc actcgacggt gatgcgcgtc ggcacgagga ccacgcccct
      121 cgccttcatg agcttgagcg tgtcttcggt gaggaagctc ccgtgctcga tggaatcgac
      181 gccggcctcg atggcgagcc gcgcggccgc atcgccatgg cagtgtgcgg cgaccttgcg
      241 ccgccaggcg tgtgcctcgg agacgatggc cttcatctct tcgagcgtga gctgcggtac
      301 gtcgaccgga tcggactcgg acagcacgcc gccgctcgcg gcaatcttga tgacgtccgc
      361 gccccatttc atctggtagc gcaccgcctc gcggcattcc tccggaccgc tgcagatacc
      421 ctggatgggt ccttcggggt ggacgcggtc cggcggatag gacagctggt cgaaatgtcc
      481 gcccggtgag ccgatgccgt gcgcggacac gagcatgcgc gggccctcgg tgatgccggc
      541 gttgatcgcg ttgcggaggc tgatgtcctc gaagtccgcg gcgcccacgt tgcgtatcgt
      601 ggtgaacccc gcttcgagtg tgcgcttggc gtagagggcg ccgtagagca cctgctcggc
      661 gggaaagcgc atcaactcgt gatagcgcat gaggtagtag ttcttctcga actgcatggt
      721 gacgtgcgtg tgcgcgtcga tgaagccggg caggagggtg gcgtcgccga gatcgatgac
      781 gcgcgcgccc gccggggcgt tggccgcccc gaccgacacg atgcgctcgc cctgcacggt
      841 gaccacgccg ccgtccctga gcgccccgtc cctggcatcg aagatgtggg ccgccttgag
      901 cacaatgatc ccggccggct gtgtctgcgc gacggccggc ggagccagcc cgagcaccag
      961 ggccgctacg gcgagcaggg gggcgggaac ccttgggact gtcatcgtgg tctcccttcc
     1021 gatcatcagg tcgtgtgctc gaccgcgaag ttcgcgagcg cggcgaggcc ggtcttataa
     1081 gaggagtcgg gcagcagcgc cagcgtgcgc agcgccgcgt ctgcctcggt gcgcgccagg
     1141 cgcgctgcgt agtcgagccc gccggtggat tcgatggcgt cgacgacgtc cccgaggtgg
     1201 ccgcggccgc cgctctggat cgctgcgcgg atcgctgcgc gctgcgtctc gctgccgtgc
     1261 ctcagggcat ggatgagggg cagcgtcggc ttgccttcgg cgagatcgtc gccgaggttc
     1321 ttgccgcgct cggccggggt cgagcagtag tcgagtacat cgtcgaccag ctggtaggcg
     1381 gtgccgagat gacggccgta ggcggccagc gcggcgcgcc gctccgccgc tgagctcgcg
     1441 agcaccgccg cgacctcggc gccggcctgg aacaagcgcg cggtctttcg gtagatgacc
     1501 tcgagatagc gctgctcggt ggtgtcggga tcgtgtgcgt tcatcagctg cagcacttcg
     1561 ccttcggcga tcacattggt cgcatcggcc atgatctcca tcaccggctg cgaggcgagc
     1621 gccgccatca tctggaaggc gcgcgagtag acgaagtcgc cgacgaggac gctcgcctgg
     1681 ttgccgaaca cttcgttggc ggtatcgcgg ccgcggcgga gcgaggagcc gtcgaccacg
     1741 tcatcgtgca gcagtgtggc cgtgtggatg aactcgatga atgccgccgc ctcgatgtgc
     1801 gcgccgccgc cctggccgca cgcccgcccg gcgaggacca ccagcagcgg gcgcagccgc
     1861 ttgccgccgc cggcgatgat gtggtcggcg acgcggtcga cgagcggcac ggcgctgctc
     1921 aggcgtgcgc gaatgacctc gtccacggag ttgaggtccg cctgaaccag ggcgcgaatc
     1981 tctgcgaggg tcacgcggcg catcctagcc gacctcagac gtcgaggaga acatcacgga
     2041 acctcactat cctcgattaa gtgcctgcca ggcttgagga aatggcggtt tgaccgggag
     2101 gggtccggta agtagaatgc gcggcctttc tttgggccca tcactcttcc agccactatg
     2161 tacgctgtca tcgccacggg cggcaagcag tatcgggtga cgaaagacgg cgtgctgcgc
     2221 gtcgagaagc tcgacgcgga gccgggcgct accgtcgagt tcggcgaggt gcttctggtc
     2281 ggggaggggg cggacgtcaa ggtgggaaag cccacgctga agggcggcaa ggtgctcgcc
     2341 accgtcgtgc gccacggcaa gggtgccaag gtgtcgatcg tgaagttcag gcgccgtaaa
     2401 cactatctgc ggcagaagaa tcatcggcag ctctacaccg aagtgaaggt cacggacatc
     2461 cgggcgggct aaggtcatgg cacacaaaaa ggcaggcggc agtaccaaga acgggcgcga
     2521 ctcccattcc aagcggctgg gagtgaagcg tttcggcggc gagcaggtgc tggctggcaa
     2581 catcatcgtg cgccagcgcg gcacgcaggt gcgcccaggc gcgaacgtcg gcgtcggcac
     2641 cgatcacacg ctgttcgcga aggtgaccgg caaggtggag ttccgtaaga agggtgccga
     2701 gcagcgcctc tacgtgagcg tactgcccga gccgcagaag gcctgagcgc ggccgccttc
     2761 gggccggcac tcggagaaag ccccggccgc ggcgccgggg tttttatttg ggctgttgag
     2821 ggcgcgcgcg ccttgcgagc taaatgaaat tcgtcgacga ggcccgagcg cgggtccagg
     2881 caggcagcgg cggacgcggc agcgcgagct tccgccgcga gaagttcgta cccttcggcg
     2941 ggccggacgg cggcgatggc ggccatggcg gcagcgtcta cctgcgtgcg gcgaccggca
     3001 tcaatacgct cgccgacttt cgcatcgaga ggacctttaa ggcggcgcac ggcgagccgg
     3061 gcggctcgaa cgactgcagc ggccggggcg gagccgatct tcacgtgccc gtgccggtcg
     3121 gcacggtggt acgcgatgcc gagacgcacg agcagctcgg ggatctgacg cacgacggcg
     3181 aggaactgct ggtggcgcgc ggcggcaagg gtggctgggg caatcaacgc ttcaagtcga
     3241 gcaccaaccg cgcgccgcgc cagttcgggc ccggcctgcc cggcgaacgg cgctcgctgg
     3301 aactcgagct caaggtcatc gcggacgtgg ggcttctcgg gcttcccaac gccggaaaat
     3361 cgacgctcat ccgcgcggtc tcggccgcac ggcccaaagt cgccgactat ccgttcacga
     3421 cgctgcaccc gaacctgggc g
//
LOCUS       DATLKL010000740         1579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_133147, whole genome shotgun sequence.
ACCESSION   DATLKL010000740 DATLKL010000000
VERSION     DATLKL010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133147"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VM713_10040"
     CDS             <1..850
                     /locus_tag="VM713_10040"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HVH84644.1"
                     /translation="WVSQVPDPWRLATAAFRGLPPERAHRLALWALRRGLTPHRDGAD
                     DPALAIRVFGLDFPNPVGLAAGFDKNAEAYAAATALGFGFVEVGSVTPQAQPGNPKPR
                     LFRLVEDRALINRIGFASEGMAVVASRLARCKAGIVGANLGKNKDSADAAADYRAGAR
                     SLAPHADYLVINVSSPNTPGLRALQGREPLAGLIAAVQQACAEVRRAEGPPILLKIAP
                     DLAAEDCRDIAEVVRMSGLDGLIIGNTRSPAHPGSRAAIAAKREACPAGRCSRSRPSC
                     SPRCTG"
     gene            826..1068
                     /locus_tag="VM713_10045"
     CDS             826..1068
                     /locus_tag="VM713_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84645.1"
                     /translation="MLAEMYRLTEGRIPLVGCGGIASGADAYAMVRAGASLVQLYTAL
                     VYDGPGLVHDLKAELALLLRRDGFASVAAAVGADHR"
     gene            1233..>1579
                     /locus_tag="VM713_10050"
     CDS             1233..>1579
                     /locus_tag="VM713_10050"
                     /inference="COORDINATES: protein motif:HMM:NF024740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVH84646.1"
                     /translation="MAPLAARYDGFILDLWGVVHDGIAAYPGAVDALARLRAAGKRVL
                     LLSNAPRRVAAIAHAMEGMGIPRALYHELLSSGEAAYDALRRRDDPWLQALGARCYLM
                     GPARDRGMVEGLDL"
BASE COUNT          212 a          563 c          551 g          253 t
ORIGIN      
        1 atgggtgagc caggtgcctg acccttggcg cctcgcgaca gccgcctttc gcggcctccc
       61 gcccgagcgc gcacaccgct tggcgctctg ggcgctgcgg cgcgggctga cgccgcatcg
      121 ggacggggcg gacgacccgg ctcttgcgat ccgcgtcttc ggtctggatt ttcccaaccc
      181 ggtcgggctg gcggccggtt tcgacaagaa tgccgaagcc tacgcggcgg cgacggcact
      241 cggattcggc ttcgtcgagg tggggagcgt cacgccccag gcccagccgg gcaatccgaa
      301 gccgcgactt tttcgcctgg tcgaggaccg cgccctcatc aaccggatcg gctttgcgag
      361 cgagggaatg gccgtggtcg cgtcgcggct ggctcgctgc aaagccggca tcgtcggcgc
      421 caatctcggc aagaacaagg atagcgccga tgccgcggcc gactaccgcg ccggcgcgcg
      481 ctcgctcgcc ccccatgccg actatctcgt catcaacgtt tcgtcgccca acacgcccgg
      541 gctgcgcgcg cttcagggtc gcgagccgct cgcgggcttg atcgccgcgg ttcagcaggc
      601 atgcgctgag gtgcggcggg ctgaagggcc gccgatcctc ctcaagatcg cgccagacct
      661 cgctgccgag gactgccgcg atatcgccga ggtggtgcgc atgagcgggc tcgacggtct
      721 catcattggc aacacacgat cgcccgccca tccgggctca cgagccgcaa tcgcggcgaa
      781 gcgggaggcc tgtccggccg gccgctgttc ccgctcgcgg ccaagctgct cgccgagatg
      841 taccggctga ccgaggggcg catcccgctt gtcggctgtg gcgggatcgc gagcggtgcc
      901 gacgcctatg ccatggtccg cgccggcgcc tcgctcgtgc agctttatac cgccctcgtt
      961 tatgacggcc ccggcttagt tcacgacctg aaggcggagc tggctcttct cctgcgccgc
     1021 gacgggttcg cgagcgttgc tgcggccgtt ggcgctgacc atcgctgagg cagaagggcc
     1081 ggcgcgccac gcgggtgcga gattacgcgg ccgggccctc gccctttgca acaccgggac
     1141 ggccatggca aaaggcgcga gccatgacaa ggacgggccc cagcgccaat agcgccgact
     1201 gtgcagcagc atcaatcccg ttgctgccgg gggtggcgcc gctggctgca cgctatgacg
     1261 gcttcatcct cgacctgtgg ggcgtggtcc atgacgggat cgccgcttat cccggcgccg
     1321 tcgacgcgct tgcccggctc agggccgcgg gaaagcgggt cctgctgctc tcgaacgcgc
     1381 cgcgccgggt agcggcgatc gcccatgcga tggaggggat gggcattccc cgcgcgctct
     1441 accacgagct tctttcctcg ggcgaggccg cctatgacgc gctccgccgc cgcgacgatc
     1501 cctggctcca ggcgctcggc gcgcgctgct acctgatggg gccggctcgc gaccggggca
     1561 tggtcgaggg cctcgacct
//
LOCUS       DATLKL010000741         3150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_166905, whole genome shotgun sequence.
ACCESSION   DATLKL010000741 DATLKL010000000
VERSION     DATLKL010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166905"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VM713_10055"
     CDS             <1..1108
                     /locus_tag="VM713_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HVH84647.1"
                     /translation="DAQYQRKIEWVRRLLEPQEGVAGEDAERDFVEGMRAELFEDRVY
                     ALTPKGEVIDLPRGATPLDFAYNVHTSLGHRCRGAKVNGRMVPLTRVLANGEIVEIIT
                     ARQEAPSRDWLAPEQSYLASARNRGKVRAWFRRQDVGDNRSAGRVIAERELARIGVRS
                     EQLAALVEELKARDLDHFHQLLGEGEITVTQLMHAASRLFQVPAPTRALRTARAAPRR
                     RRSPVEIEGVGDLPTTLARCCAPLRPQPITGYVTLGRGVTVHRSDCASLQRMSALKPD
                     RVLKVEWAAADSGALDVEITVSAYDRRGLLRDLTDVLAAERLSIDAVTSRTDHDAGVA
                     HFALSIAVGDLEQLSRVLRRLAGVPNVIEARRRA"
     gene            complement(1136..2593)
                     /gene="lpdA"
                     /locus_tag="VM713_10060"
     CDS             complement(1136..2593)
                     /gene="lpdA"
                     /locus_tag="VM713_10060"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HVH84648.1"
                     /translation="MATTDKPPAGGSDAFDVIVIGGGPAGYPAAIRAAQNQLRVACID
                     EWKNKDGSATFGGTCLNAGCIPSKALLESTELYHRAREEFAIHGIGVAGVSLDLAQMQ
                     KRKAGVVKGMTTGILALFKAAGVTPLQGHGKLLPGRRVQFTAHDGTRRELTARHVVLA
                     SGSVPIELRSAPFKAPHVVDSWGALDFDAVPKRLGVIGAGVIGLELGSVWRRLGAEVT
                     VLEALPDFLPFADQQLAKEAQRHFKRQELDIRLGAKVTGATLKVGVVEVTYSDAKGEQ
                     RTAVDRLVVAIGRRPYTQDLLDAGTGVELDERGFIKVDHSCRTGVEGVWAVGDCVRGP
                     MLAHKGKEEGAMVADLIAGRYGEVNYKVIPAVIYTAPEIAWVGLTEEQVKSSGREYKT
                     GVFPFLASARARAMEQAAGFAKIVAARDDDEILGVHIVGPLAGELIAEAVLAMEYSAS
                     SEDLQRTIHAHPTLSEAVHEAALNADKRAIDSLNR"
     gene            2759..>3150
                     /locus_tag="VM713_10065"
     CDS             2759..>3150
                     /locus_tag="VM713_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003123890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HVH84649.1"
                     /translation="MSSAAETLAAPAARPVLDRKSSPGTRLIFLALLVVGIGYSGYSL
                     VSDISGVDTRAPAPILPYFLLGVALLIALGFEFVNGFHDTANAVATVIYTHSLPPHVA
                     VVYSGTLNFLGVLLSSGAVAFGIISLLPV"
BASE COUNT          475 a         1136 c         1043 g          496 t
ORIGIN      
        1 cgatgcgcag taccagcgca agatcgagtg ggtgcggcgg ctgctcgagc cgcaggaagg
       61 cgtcgcgggc gaggacgccg agcgtgactt cgtcgagggc atgcgcgcag agctcttcga
      121 ggaccgtgtc tatgcgctga cccccaaggg cgaggtcatc gacctgccgc gcggcgcgac
      181 gccgctcgac ttcgcctaca acgtccacac ctcgctcggc caccgctgcc gcggcgccaa
      241 ggtcaacggc cgcatggtgc cgctcacccg ggtgctcgcc aatggcgaga tcgtcgagat
      301 catcaccgcc aggcaggagg cgccgagccg cgactggctc gcgcccgagc aaagctacct
      361 cgcctcggcg cgcaatcgcg gcaaggtgcg cgcctggttc cgacggcagg acgtcggcga
      421 caaccgcagc gccggtcgtg tcatcgccga acgcgagctc gcgcgcatcg gcgtgcgcag
      481 cgagcagctc gccgcactgg tcgaggagct caaggcccgc gacctcgatc acttccacca
      541 gctgctcggc gagggcgaga tcaccgtcac ccagctgatg catgccgcct cgaggctgtt
      601 ccaggtgccc gcgccgacgc gcgcgttacg gacggcgcgc gccgcgcccc gccgccggcg
      661 ctcgccggtc gagatcgagg gcgtcggcga tctgccgacc acgctggcgc gctgctgcgc
      721 gccgctgcgg ccgcaaccga tcaccggtta cgtgacgctc gggcgcggcg tcaccgtgca
      781 ccgcagcgac tgcgcgagcc tgcagcgcat gagcgcgctc aagcccgacc gcgtgctgaa
      841 ggtggaatgg gccgccgccg acagcggcgc gctcgacgtc gagatcacgg tgagcgccta
      901 cgaccggcgt ggactgctgc gcgatctcac cgacgtcctg gcggccgagc gcttgagcat
      961 cgacgcggtc acctcgcgca ccgatcacga cgcgggcgtg gcacactttg cgctcagcat
     1021 tgcggtcggc gatctcgagc agctctcgcg cgtactgcgg cgcctcgcgg gcgtccccaa
     1081 cgtcatcgag gcgcgccggc gggcctgaat agccctcggg ccggcgggtt cgggctcagc
     1141 ggttgagcga atcgatcgcg cgcttgtcgg cgttgagcgc cgcctcgtgc accgcctccg
     1201 acagcgtcgg gtgcgcgtgg atggtgcgtt gcaggtcctc gctgctcgcc gaatactcca
     1261 tcgccagcac cgcctcggcg atcagctcgc cggccagcgg accgacgatg tggacgccga
     1321 ggatctcgtc gtcatcgcgc gccgcgacga tcttggcaaa gcccgccgcc tgctccatgg
     1381 cgcgcgcccg cgcgctcgcg aggaacggaa acaccccggt cttgtactcc cgtccgctcg
     1441 acttgacctg ctcctcggtg agcccgaccc aggcgatctc gggcgcggtg tagatcaccg
     1501 ccggaatcac cttgtagttg acctcgccat aacgccccgc aatgaggtcg gccaccatgg
     1561 cgccctcctc cttgcccttg tgggccagca tcggaccgcg aacgcagtcc cccaccgccc
     1621 acaccccctc gacgcctgtg cggcaggagt gatcgacctt gatgaagcca cgctcatcga
     1681 gctcgactcc ggtgcccgca tccagcaggt cctgcgtata gggccggcgc ccgatcgcca
     1741 ccaccagccg atcgacggcg gtgcgctgct cgcccttcgc gtcgctgtag gtgacctcga
     1801 cgacgccgac cttcagcgtc gcaccggtca ccttggcccc gaggcggatg tcgagctcct
     1861 gcctcttgaa atgccgctgc gcctccttgg cgagctgctg atcggcgaag gggagaaaat
     1921 ccggcagggc ctcgagcacg gtgacctcgg cgccgagccg ccgccagacg cttccgagct
     1981 cgagcccgat cacacccgcg ccgatgacgc cgaggcgctt cgggaccgca tcgaaatcga
     2041 gcgcgcccca ggagtcgacc acgtgcggcg ccttgaaggg cgccgagcgc agctcgatcg
     2101 gcactgagcc cgaggcgagc actacgtgcc gcgcggtgag ctcgcggcgc gtgccgtcgt
     2161 gggcggtgaa ctgcacacgg cggccgggca agagcttgcc gtgcccctgg agcggcgtca
     2221 cgccggcggc tttaaagagt gccaggatcc cggtcgtcat ccccttgacg accccggcct
     2281 tgcgcttctg catctgcgcg aggtcgagcg agacaccggc gacgccgatc ccgtggatgg
     2341 cgaactcctc gcgggcgcga tggtagagct cggtggattc gagcagcgcc ttcgagggaa
     2401 tgcagccggc gttgaggcac gtgccgccga aggtggcgct gccgtcctta ttcttccact
     2461 cgtcgatgca ggcgacgcgc agctggttct gtgccgcgcg gatcgccgcc ggataaccgg
     2521 ccggcccccc gcctatcacg atgacatcga aggcatcaga tccgcccgca ggcggcttgt
     2581 cggtggtcgc catggaagtg cttgtaaagt ctggggttag tggttcttca ggaccgagga
     2641 aagtaacaca aacggaatgg gccgggtgta gctgggcacg ccctttcgac gtggggtcat
     2701 aaaagcgaaa tatcaacgtc atagggtttc gcgacttacc gaaccgcgag gacgcccgat
     2761 gagctccgcc gccgagaccc ttgccgcgcc tgccgcccgc cccgtcctcg atcgcaagtc
     2821 cagccccggc acccggctga tattcctcgc gctgctcgtc gtcggcatcg gctacagcgg
     2881 ctacagcctg gtgagcgaca tcagcggcgt cgacacgcgc gcccccgcgc cgatcctgcc
     2941 ctactttctt ctcggcgtgg cgctgctcat cgcgctcggg ttcgagttcg ttaacggctt
     3001 ccatgacacg gccaacgccg tcgccaccgt catctatacc cattccctgc cgccgcacgt
     3061 ggcggtggtg tactcgggca cgctgaattt tctcggcgtg ctgctctcga gcggcgcggt
     3121 ggcctttggc atcatctcgc tgctgccggt
//
LOCUS       DATLKL010000742         1705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_20116, whole genome shotgun sequence.
ACCESSION   DATLKL010000742 DATLKL010000000
VERSION     DATLKL010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20116"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VM713_10070"
     CDS             <1..262
                     /locus_tag="VM713_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HVH84650.1"
                     /translation="GDWIVIEAVANAFLHHMVRNIAGLLIAVGRGEEPPGWARAVLEG
                     RDRTRGAATAPAAGLYLWEVRYPEAFGLPAPAPEGPQSPDQL"
     gene            259..1170
                     /gene="accD"
                     /locus_tag="VM713_10075"
     CDS             259..1170
                     /gene="accD"
                     /locus_tag="VM713_10075"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HVH84651.1"
                     /translation="MIEAPLELDAVPQSARMTWFEKIMPSRIKTERRTRSVPEGLWLK
                     CPACDAVLYRAELERNLYVCPKCSHHMRIRARERLERFLDQGTIGEIGANIAPEDPLK
                     FRDSKRYRDRLTQAQKATGESDALVVLEGALHGLPAVAAAFEFQFLGGSMGSVVGERF
                     KRGVDYCIQERRPFICFSSSGGARMQEALYSLLQMAKTAASLSRLAQAHLPFVSVLTD
                     PTTGGVSASLALLGDLNIAEPRALIGFAGPRVIQQTVRETLPEGFQRAEFLLEHGAID
                     LIIDRRDMRDRIAALLRLLLKLPVAAA"
     gene            1203..1667
                     /locus_tag="VM713_10080"
     CDS             1203..1667
                     /locus_tag="VM713_10080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84652.1"
                     /translation="MRTLAEWLELQDSVHPRSIDLSLERVTEVAERLGVTAPAFRLIT
                     VGGTNGKGSVAAHAEALLAALGTRVGLFTSPHLVRYNERIRVRGQEASDAELIEAFER
                     IEVARGATTLTFFEYNTLAALLVFTHRAVEVAVLEVGLGGRLDATNLVQNDV"
BASE COUNT          253 a          604 c          573 g          275 t
ORIGIN      
        1 aggcgactgg atcgtcatcg aggcggtcgc caacgccttc ctccatcaca tggtgcgcaa
       61 catcgccggc ttgcttatcg ccgtcggcag gggcgaagag ccgcccggct gggcgcgcgc
      121 cgtgctggag gggcgcgacc gcacgcgtgg agccgccacc gcgcctgccg ccggcctcta
      181 tctctgggag gtccgctacc cggaggcctt cgggctgccg gctcccgccc cggaagggcc
      241 gcagagcccc gatcagttat gatcgaagcg ccgctcgagc ttgacgcggt gccgcagagt
      301 gcccggatga cctggttcga gaaaatcatg ccctcccgca tcaagaccga gcgtcgcacg
      361 cgctcggtgc cggaggggct gtggctcaag tgcccggcct gcgatgccgt gctctatcgg
      421 gccgagctcg agcgcaacct ctatgtgtgt ccgaaatgca gccaccacat gcgcatccgc
      481 gcgcgcgagc gcctcgagcg cttcctcgat cagggcacga tcggggagat cggcgcgaat
      541 atcgcgcctg aggatccgct caagttccgc gacagcaagc gctaccgcga ccgcctcacc
      601 caggcgcaga aggcgactgg cgagagcgat gcgctggtgg tactcgaagg cgcgctgcat
      661 gggctgccgg cggtcgcggc cgccttcgag ttccagttcc tcggcggctc gatggggtcg
      721 gtggtcggcg agcgcttcaa gcggggcgtc gactactgca tccaggagcg gcggccgttc
      781 atatgctttt cctcgagcgg cggggcgcgc atgcaggagg cgctgtattc gctgctgcag
      841 atggccaaga ccgccgcctc gctctcgcgc ctcgcgcagg cgcacctgcc tttcgtctcc
      901 gttctgaccg atccgaccac cggcggggtg tcggcgagcc tcgcgctcct cggggacctc
      961 aacatcgccg agccgcgggc gctcattggc ttcgcaggcc cgcgcgtcat acagcagacc
     1021 gtgcgcgaga ccctccctga gggcttccag cgcgccgagt tcctgctcga gcacggcgct
     1081 atcgacctga tcatcgaccg gcgcgacatg cgcgaccgga tcgcggcgct cctgcggctg
     1141 ctcctcaagc ttccggtggc ggccgcctga agagcgggtc cgccgcgcct caaacgctca
     1201 agatgcgcac gctcgccgag tggctcgagc ttcaggactc ggtccacccg cgtagcatcg
     1261 acctgagtct cgagcgggtc acggaagtcg ccgagcgcct cggggtcacg gccccggcct
     1321 tccgcctcat caccgtaggc ggcaccaacg gcaagggctc ggtggccgcg cacgccgagg
     1381 cgctgctcgc cgcgctcggt acacgggtgg gtcttttcac ctcgccccac ctcgtccgct
     1441 acaacgagcg aatccgcgtg cggggccaag aggcgagcga tgcggagctc atcgaagcgt
     1501 tcgagcgcat cgaggtcgcg cgcggtgcga cgacactcac cttcttcgag tacaacactc
     1561 tcgccgcgct gctcgtcttc acgcatcgcg cggttgaggt cgcggtgctg gaggtcggcc
     1621 tcggcggtcg cctcgatgcc accaacctgg tccaaaacga tgtctgatgc cggcgacttg
     1681 ctcaacggcg gcagcgacac tgtgg
//
LOCUS       DATLKL010000743         1875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_42815, whole genome shotgun sequence.
ACCESSION   DATLKL010000743 DATLKL010000000
VERSION     DATLKL010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42815"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /gene="cobA"
                     /locus_tag="VM713_10085"
     CDS             <1..921
                     /gene="cobA"
                     /locus_tag="VM713_10085"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HVH84653.1"
                     /translation="VRRALAPEARRAFWERVIGGRAAAQMLGGDPAAAARSFASELRA
                     SGSGPGEVYLIGAGPGDPDLLTLRALQLLQQADVILYDRLVPEPILERARRDAERVFV
                     GKETGDHAQQTRINELLVGLARAGKRVARLKGGDGYVFGRGGEELEALAAAGIPWRVV
                     PGITAALGAAAAADIPLTHRRLAHSVTLVSGHAAADDSLDWRALARSGQTVVFYMGVA
                     QLPRIVARLRAAGASAELPAAIVERATLPGQRVLRAPLAALATLAVRERVAAPALLIV
                     GEVTALATAAGLAAAGPAVPSDRAAVGAVA"
     gene            918..>1875
                     /locus_tag="VM713_10090"
     CDS             918..>1875
                     /locus_tag="VM713_10090"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF007989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HVH84654.1"
                     /translation="MSSVAGFVGAVGNTPLIRLRRVSEESGCEIPGKAEFMNPGGSVK
                     DRAAAAIIAAAERRGQLRPGGIVVEGTAGNTGIGLAHVCNARGYRCIIVMHDNQSPEK
                     YRLLEMLGAEVHKVPVVPYSNPNQYQKVAERLAASLPGAIWSNQFDNTANRDTHIATT
                     GPEIWTQTGGRIDAFVAATGTGGTLAGVAHYLKSKSRAVRCVLADPPGSSLYEYFRSG
                     TLKATGSGSITEGIGIARITANLKDAPIDAAVHVPDADTVRCVYRLLYEEGLFLGSTS
                     GINVAAALQVTRELGPGATVVTVLCDGGAKYQSRLFNRVWLEE"
BASE COUNT          273 a          682 c          667 g          253 t
ORIGIN      
        1 gtaaggcggg cgctcgcccc cgaggcgcgg cgcgccttct gggaacgggt catcggcggg
       61 cgcgccgccg cgcaaatgct cggcggcgac cctgccgcgg ccgcccgcag cttcgcgagc
      121 gaattgcgcg cttcaggcag cggtcccggc gaggtctatc tcatcggcgc aggtcccgga
      181 gacccggacc tgctcacgct gcgcgcactg cagctgctgc agcaggcgga cgtaatcctc
      241 tacgaccggc tggtgccgga gccgatcctc gagcgcgcgc gccgcgacgc cgagcgggtg
      301 ttcgtcggca aggagaccgg cgaccatgcg cagcagacgc gcatcaacga gctcctggtc
      361 ggcctggccc gtgccggcaa gcgcgtcgcc cgcttgaagg gcggcgacgg ttacgtgttc
      421 ggccgcggcg gcgaggagct cgaagcgctg gccgccgccg gtatcccctg gcgggtcgtg
      481 cccggaatca ccgcggccct gggagctgcg gccgccgcgg acattccgct cacacatcgc
      541 cggctcgcgc acagtgtgac gctcgtgagc ggccacgcag ccgccgatga ctctctcgat
      601 tggcgcgcgc tggcgcgctc cggtcagacg gtagtgttct acatgggcgt ggcgcagctg
      661 ccgcgcatcg tggcgcggct gcgtgccgca ggagcgagcg cggagctgcc cgccgccatc
      721 gtcgagcgtg cgacgcttcc cggccagcgc gtgctgcggg cgccgcttgc ggcgctcgcc
      781 acgctcgcgg tccgcgagcg ggtggcagcg ccggcgctgc tgatcgtggg cgaggtcacc
      841 gcactggcga ccgcggcagg tctcgcggcg gcaggtccgg cggtgccatc cgatcgcgcc
      901 gccgtcggag ccgtggcgtg agcagcgtcg ccggcttcgt cggcgcggtg ggcaacacgc
      961 ccctcatccg cctccgccgc gtgagtgagg agagtggctg cgagatcccc ggcaaggcgg
     1021 agttcatgaa ccccggcggc tcggtcaagg accgggccgc ggccgccatc atcgccgccg
     1081 ccgaacggcg cgggcagctc cgccccggcg gcatcgtcgt ggagggaacc gccggcaaca
     1141 ccggcatcgg gctggcacac gtctgcaacg cgcgcggcta ccgctgcatc atcgtcatgc
     1201 acgacaacca gtcgcccgag aagtaccggc tgctcgagat gctgggtgcc gaggtgcaca
     1261 aggtaccggt cgtcccctac agcaacccca accagtatca gaaggtcgcc gagcgcctgg
     1321 cggcctcgct cccgggagcg atctggtcga atcagttcga caacaccgcc aaccgcgaca
     1381 cgcacatcgc cacgacggga cctgagatct ggacgcagac cggcgggcgc atcgatgcct
     1441 tcgtggcggc gaccggcacc gggggcacgc tcgccggcgt cgcgcactat ctcaaatcga
     1501 agagccgtgc ggtgcgctgc gtgctggccg acccgcccgg cagcagcctg tacgaatact
     1561 tccgcagcgg tacgctcaag gcgaccggca gcggctcgat caccgaaggc atcggcatcg
     1621 cccgcatcac cgccaatctc aaggacgcgc cgatcgatgc cgcggtgcac gtgccggacg
     1681 ccgacaccgt ccgctgcgtc taccggctgc tctacgagga gggcctgttc ctcggcagca
     1741 ccagcggcat caacgtcgcc gcggcactcc aggtgacacg cgagctcggt ccgggggcca
     1801 ctgtcgtgac cgtgctctgc gatgggggcg ccaagtacca gtcgcgcctc ttcaaccgcg
     1861 tctggctcga ggaga
//
LOCUS       DATLKL010000744         3010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_222853, whole genome shotgun sequence.
ACCESSION   DATLKL010000744 DATLKL010000000
VERSION     DATLKL010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222853"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..420)
                     /locus_tag="VM713_10095"
     CDS             complement(25..420)
                     /locus_tag="VM713_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVH84655.1"
                     /translation="MPGPDLDVERVVCRETELDVHGARAFTIGAGDWPLRGFVVRVGA
                     HLRGYVNRCPHAGHPLNLLPDRFCTPDGALLLCSSHGALFEKLTGYCVAGPCAGRALT
                     PVPLEVRSGFVMVADGVDIAALAESEKIA"
     gene            complement(407..1807)
                     /locus_tag="VM713_10100"
     CDS             complement(407..1807)
                     /locus_tag="VM713_10100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH84656.1"
                     /translation="MSADSSETRLAGGSGMAASSRGWVQALRVYAQPRVVSMLFLGFS
                     AGLPFYLVFSTLSAWLRQAGIQRSTIGMLSWVGILYSIKFLWAPVVDRMPLPVLQRLL
                     GRRRSWMLLAQLGIVAGLARLADSDPSAGVTSMALAALGVAFCAATQDIALDAWRIES
                     AEVSMQGAMVAAYQIGYRVALISGSAGALTIAARANWHASYTTMAVLGGVGIVTTLLI
                     REPPPVVSAAAEQREARVIDWLERNAHWPQWARHAGEWFIAAVICPLTDFFQRYGARL
                     AIVTLCFIGVYRLTGFTMGSMANPFYIDHGYTLAQIATVVKVYGLIMSMFGVVAAGVF
                     VARFGLTRSLVLGSVLLMASNLAFATLATTHEPTVLGLALVNGLDNLAIAAQGVALIA
                     FLSSLTSQRYTATQYALFSSLYALPGKILEGTSGFVVDQVGYPVFFTYTAALSLPGLI
                     LLYFLNRRGVVGRART"
     gene            complement(1804..2565)
                     /locus_tag="VM713_10105"
     CDS             complement(1804..2565)
                     /locus_tag="VM713_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HVH84657.1"
                     /translation="MITVNVCGIRSAAAKGLFRWLRRQRADFICLQETRCQEDQLDGH
                     DVQLPGYRSFFSQAERKGYAGVALYTRHHVDRVLRGFGVPEFDREGRYLEARLGELSV
                     VSLYLPSGSAGPERQASKFRFLEAFTPHLARLKRGRRRCILSGDWNIAHRAIDLRNWK
                     SNQKNSGFLPEERAWMDRLFGPAGYVDAFREVRPGPNLYTWWSNRGQARANNVGWRID
                     YQVVSGSLAGKARAAFIYKERRFSDHAPLTMDYDL"
     gene            2590..>3010
                     /gene="pyrE"
                     /locus_tag="VM713_10110"
     CDS             2590..>3010
                     /gene="pyrE"
                     /locus_tag="VM713_10110"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HVH84658.1"
                     /translation="MQDYQLTFIELALQREALRFGQFRLKSGRDSPYFFNAGLFADGE
                     AAAVLGRCYAAAVVRSGLEFDMLFGPAYKGIPLATAAAIGLALHHGRSVPYAYNRKEA
                     KEHGEGGVVVGAPPHGRVLIVDDVITAGTAVRESLELI"
BASE COUNT          555 a         1008 c         1009 g          438 t
ORIGIN      
        1 agctgatgtc cggaatagtc gccactaggc gattttctct gattcagcaa gcgccgcgat
       61 atccaccccg tccgcgacca tcacgaaccc gctgcgcact tcgagcggga ccggcgtgag
      121 cgcgcggccc gcgcaaggcc ctgccacaca gtaacctgtg agcttctcga acagcgcgcc
      181 gtgcgaggag cacagcagca acgcgccatc cggcgtgcag aatcggtcgg gcaggagatt
      241 caacggatgg ccagcgtgcg ggcagcggtt cacgtagccg cgcaggtgcg cgcccacccg
      301 caccacgaaa cccctgagcg gccagtcgcc cgcgccgatg gtgaaggccc gcgccccgtg
      361 cacgtcgagc tcggtctcgc ggcagaccac gcgctcgacg tcgaggtcag gtccgggcac
      421 ggccgaccac gccacgacga ttaaggaagt acagcaggat cagtccgggc aagctcaagg
      481 ccgcggtgta agtgaagaac accggatagc ctacttggtc gactacgaat ccggaggtcc
      541 cttcgaggat ctttcccggc agtgcgtaca gcgaggagaa cagcgcgtat tgcgtggccg
      601 tataacgctg gctcgtgaga ctcgacagga acgcgatgag cgccacgccc tgagccgcga
      661 tcgcaaggtt gtccagtccg ttaaccagcg ccaatcccag caccgtcggc tcgtgtgtcg
      721 tcgcgagcgt cgcgaacgcg aggttcgagg ccatgaggag cacgctgccg agcaccagcg
      781 agcgcgtgag tccgaatctg gcgacgaaga cgccggccgc cacgacgccg aacatcgaca
      841 tgatgaggcc gtacaccttc acgacggtgg cgatctgcgc cagggtgtag ccgtgatcga
      901 tgtagaaggg gttcgccatc gatcccatcg tgaatccggt gaggcgatac acgccgatga
      961 agcacagcgt gacgatcgca agtcgggcgc cgtagcgctg aaaaaaatcc gtgagagggc
     1021 agatgaccgc tgcaatgaac cattcgccgg cgtggcgcgc ccactgcggc cagtgcgcgt
     1081 tgcgctcgag ccagtcgatg actctcgcct cacgttgctc ggcagcggct gagaccacgg
     1141 gaggcggctc gcgaatgagc agcgtcgtga cgattcccac gccgccgagc accgccatgg
     1201 tcgtgtagct tgcgtgccag ttggcccggg cggcgatggt gagggcaccg gcacttcccg
     1261 agatcagggc gacccggtag ccgatctggt aggcggcgac catcgcgccc tgcatggaca
     1321 cttcagcgga ctcgatgcgc cacgcatcca gcgcgatatc ctgcgtcgcg gcacagaagg
     1381 cgacgcccag cgcggcgagc gccatggagg taacaccggc ggaagggtcg ctatcggcaa
     1441 gtcgcgccag gccggcgacg atgcctagct gggcgagcag catccaggag cgccggcgac
     1501 cgagcagccg ttgcagaacc ggcagcggca tgcgatcgac gaccggcgcc cacaggaact
     1561 tgatcgaata gagaatgccg acccacgaca gcatgccgat cgtgctgcgc tggatccccg
     1621 cttgacgcag ccacgccgac agcgtggaga acaccaggta gaaaggtaac ccggccgaga
     1681 agccgaggaa cagcatcgat accacgcggg gctgcgcgta cacgcgcagc gcctgcaccc
     1741 agccgcggct ggaagccgcc atgcctgagc cgcccgcgag gcgtgtctcg ctcgaatccg
     1801 cgctcaaaga tcgtagtcca tggtgagcgg ggcgtgatcc gagaaacgcc tctccttgta
     1861 tatgaaggcg gcgcgggctt tgccggcgag cgatccgctc acgacctggt agtcgatgcg
     1921 ccagccgacg ttgttcgcgc gcgcctgccc gcggttcgac caccacgtat agaggttggg
     1981 ccccgggcgc acctcccgga atgcgtccac gtagccggcg ggaccaaaca ggcgatccat
     2041 ccaggcgcgc tcctcgggga gaaagccgga gttcttctgg ttcgacttcc agttcctcaa
     2101 gtctatggcg cggtgcgcga tgttccagtc gccactcagg atgcagcgcc gccgcccgcg
     2161 cttgagccgc gccaggtgcg gcgtgaaggc ctccaggaag cgaaacttcg aggcctggcg
     2221 ctcgggtccc gcggagcccg acgggaggta gagtgagacg accgacagct caccgaggcg
     2281 cgcctcgagg tagcgcccct cgcggtcgaa ctccggcacg ccgaagccgc ggaggacgcg
     2341 gtccacatga tggcgggtgt agagcgcgac cccggcgtag cccttgcgct cggcttggct
     2401 gaagaagctc cggtagccgg gcagctggac gtcgtgcccg tcgagctgat cctcctggca
     2461 gcgcgtctcc tgcaggcaga tgaaatccgc gcgttggcgc cgcagccagc ggaacaggcc
     2521 cttcgccgcc gcggagcgga tgccgcacac gttgacggtg atcactcgca agccgaacaa
     2581 tgatatgtca tgcaggatta ccagctcacc ttcatcgagc ttgcgctcca gcgcgaggcg
     2641 ctgcgcttcg gacagtttcg cctcaagtcc ggccgcgaca gcccctactt cttcaacgcc
     2701 gggctgttcg ccgacggcga ggcggcggcg gtgctcgggc ggtgttacgc ggccgccgtg
     2761 gtccgctccg gcctcgagtt cgacatgctc ttcggccccg cctacaaggg cattccgctc
     2821 gccaccgctg ccgcgatcgg gctcgccctg caccacggcc gcagcgtccc gtacgcctac
     2881 aaccgcaagg aggccaagga gcacggcgaa ggcggtgtgg tcgtcggcgc gccgccccac
     2941 gggcgcgtgc tgatcgtcga cgacgtgatt acggcgggca ccgcggtgcg cgagtcgctc
     3001 gagctgatcc
//
LOCUS       DATLKL010000745         2915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_31572, whole genome shotgun sequence.
ACCESSION   DATLKL010000745 DATLKL010000000
VERSION     DATLKL010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31572"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VM713_10115"
     CDS             complement(<1..530)
                     /locus_tag="VM713_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84659.1"
                     /translation="MTENPPTRDLLHIAQTVGVVLTSASALAYASGYFVLRARAHALG
                     TDPGFTLVDQAYVFAGFRFALLTLIALLASAPLLILSRAIGRRVNEISSRTLRVALEC
                     VAALALIGVTLAGFVTLEVSGVLVSDRPSSTALDSAILGEGRLGVLISLGATYAAAAS
                     VLWSRARYLAHGATDTL"
     gene            complement(527..>2915)
                     /locus_tag="VM713_10120"
     CDS             complement(527..>2915)
                     /locus_tag="VM713_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HVH84660.1"
                     /translation="LRRGLPGLQALAERGTPEIRASALIEIAFIQRVSNDFGRAAESY
                     TRAASIAAPLGSSDILFDAWMGAARAMGSTRNHGAAAEALERAIAAAGAHPTRKQHFD
                     VALYSAELAAERGEIESSLVSALDALHWAPAADDRFYAELDAASALETLAESCDYRLL
                     HDSRSDNDPPDDPWGACKRTLAASTAGYLRAAQTANALGWRSLVAQLQDANVRVKARR
                     QLLDGRAHGLPDPRANPQLSADFVKFTPRSRKDVLVERGEMARKYLAASVTGPSLNQQ
                     LLGLMEHTITNAVEAQGAEDPSALIMRGDLENARTGNTARATDLFARASQLLMKERAS
                     FFDPRRRGTVVERQGGLLAKLALTLLSQRRDDDAFSVFELGRARGLGELGEILGRKDV
                     SLSDRAWLAQQVKLDAQTSAAEQRVVETVIGEGSLERPAEELGRWEATGGERRLHLLQ
                     RADLRDRFARSSFAPARLEELQHAATASGIPVLLYWVDNPNVYTWYVGPHGSDFAIIF
                     LPAAALKPKIEQVRAIADESGSLNEAAARQLYLYLIAPFSDVLDAQQLLIVPQGELVG
                     LPFEVLKDPDSDRFLIEQHIISYAPNASAALRSLQRTLPAVRAVTAIVDLEIDDGTHE
                     TSRIRAISGLQLRVLSADDVVPGKLVQSLRGAQAVHILLHGQFNALEPLLSTLGATVK
                     GTPSLEAADLLAIPLRGTRLVVMSACESGELQRRVSNEIYGFPWVLLAAGAENVVTSR
                     WRVNGASNGEWMKSFYAAVASGAPPAAAAAGAMRTMLKASHTSPYFWAAMQVTGR"
BASE COUNT          493 a          959 c          980 g          483 t
ORIGIN      
        1 agcgtgtcgg tcgcgccatg ggcgaggtaa cgcgcccggc tccagagcac ggacgcggcg
       61 gctgcgtaag ttgctcccag tgagatcaga actccgagcc ggccttctcc caggatcgcg
      121 ctatccaaag cggtcgatga aggtctgtcg ctcaccagga cgccgctcac ttcgagcgtc
      181 acgaagccgg cgagagtcac ccctatcaag gcaagagccg caacacattc cagggcgacg
      241 cgcagcgtgc gggagctaat ctcgttcacg cgccgtccga tggcacggct cagtatgaga
      301 agcggggcgc tcgccagcag cgcgataagc gtgagcagcg cgaagcgaaa tcccgcgaat
      361 acatacgcct gatccaccag cgtgaaaccc gggtcggtgc cgagcgcgtg ggcgcgcgcg
      421 cgcagcacga aatagccgct cgcgtaggca agcgccgagg ccgaagtgag caccacaccc
      481 accgtttgag cgatgtgtaa caggtcgcgg gtgggcgggt tctcggtcac cgcccggtta
      541 cctgcatggc cgcccagaaa tacgggctgg tgtggctcgc cttcagcatc gtgcgcatgg
      601 ctcccgccgc ggctgctgcc ggcggcgcgc cgcttgcgac ggccgcgtag aaggacttca
      661 tccactcgcc gttgctcgcg ccgttgacgc gccagcgtga agtcaccaca ttctccgcac
      721 cggcggccag caacacccag ggaaagccgt aaatctcgtt ggatacgcgg cgttgcaatt
      781 cgccgctctc gcaagcgctc atgacgacga gtctcgttcc gcgtagcggt atggccagca
      841 gatctgccgc ctccagagac ggcgtgccct tgaccgtggc gcccagcgtg gacagcagcg
      901 gctccagcgc gttgaactgg ccgtgcagga gaatatggac ggcctgcgcg ccgcgcaagc
      961 tttggaccag cttgccggga acgacgtcgt cggcactgag cactcgcagt tgcagacccg
     1021 agatcgcgcg gatcctgctc gtttcgtgcg tgccgtcgtc gatctcgagg tcgacgatcg
     1081 cggtcacggc tcgcaccgcg ggcaacgtgc gctgcagaga gcgcagcgcc gccgatgcgt
     1141 tgggtgcgta ggagatgatg tgttgctcga tgaggaagcg gtcgctgtcc gggtccttta
     1201 gcacctcgaa cggcaggccg acgagctctc cctgaggcac gatcagcaac tgttgcgcgt
     1261 cgaggacatc agaaaacggc gcgataaggt aaagatacag ctgccgggct gccgcttcgt
     1321 tcagggagcc gctttcatcc gcgatcgcac gaacctgctc gatcttgggc ttcagcgccg
     1381 cagccggcag aaagatgatg gcgaaatccg agccgtgcgg gcccacgtac caggtgtaca
     1441 cgttgggatt gtcaacccag tagagcagca ccggaattcc gctggcggtg gccgcgtgct
     1501 gcagctcctc gagtcgtgcg ggcgcgaagc tcgagcgcgc gaagcggtca cgaagatcgg
     1561 cccgctgcag caggtgcagc cgtcgctcgc cgccggtcgc ctcccaacgg ccgagctctt
     1621 cggcagggcg ctcgaggctc ccctcaccga tgacggtctc gaccactctc tgctccgccg
     1681 cgctcgtctg cgcgtccagc ttgacctgct gggcgagcca ggcgcggtca ctgagtgata
     1741 cgtccttgcg gccgaggatc tcgccgagtt ctcccagacc gcgggcgcga cctaattcga
     1801 agacgctgaa ggcgtcgtcg tcccttcgtt gggacagcag agtcagcgcg agcttcgcga
     1861 gcagcccgcc ttgcctctcg accacggtac ctcggcgcct agggtcgaag aagctggcac
     1921 gctccttcat cagcagctgg ctcgcgcgag cgaacaggtc ggtggcgcgc gcggtattcc
     1981 cggtgcgcgc gttctccagg tccccacgca tgatcagcgc actgggatcc tccgcgccct
     2041 gggcctccac ggcattcgtg atggtgtgct ccatcagccc gagcagctgc tgattaagag
     2101 acgggccggt cacggaagcg gcgagatact tgcgagccat ctcgcccctt tccacgagca
     2161 cgtccttgcg gctgcgggga gtgaacttca cgaagtcggc gctgagttgg gggttggcac
     2221 gtgggtccgg cagcccgtgc gctcggccgt ccaagagctg acgacgcgcc ttcacgcgga
     2281 cattcgcgtc ctgcagttgg gcgaccagcg atctccagcc gagggcgttg gcggtctgcg
     2341 cggcacgaag atagccggct gtcgaggcgg cgagtgtccg cttgcaagcg ccccaggggt
     2401 cgtcgggcgg atcgttgtcg ctgcggctgt catgcaagag ccggtagtcg caactttccg
     2461 cgagggtctc aagcgcactg gccgcgtcga gctctgcgta gaagcggtca tcggcggccg
     2521 gtgcccaatg cagcgcgtct agagcgctca ccagggagga ttcgatctcg ccccgctcgg
     2581 cggcgagctc tgcggagtac agggcgacgt cgaagtgctg cttccgggtc ggatgggcac
     2641 cggccgcagc aattgcacgc tccagagctt cggcggcggc tccgtgattg cgcgtcgacc
     2701 ccatcgcgcg ggccgcaccc atccaggcat cgaacaggat gtcgctacta ccaagaggcg
     2761 cggcgatgct ggcggcccgg gtgtagctct ccgccgcccg cccgaagtca ttcgaaaccc
     2821 gctgaatgaa ggcgatctcg atcagggcgc tggcgcggat ctccggagtg cctctctcgg
     2881 ctagtgcctg gagtccggga agaccccgcc gcagg
//
LOCUS       DATLKL010000746         1986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_200664, whole genome shotgun sequence.
ACCESSION   DATLKL010000746 DATLKL010000000
VERSION     DATLKL010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200664"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VM713_10125"
     CDS             complement(<1..574)
                     /locus_tag="VM713_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVH84661.1"
                     /translation="MNSPLQTMSTAPPLPAQRLDAAGRAHVDSRAVLALALPLMANSA
                     VQIVLNLTDMWFIGRISTKALAAVGAVQWLVVVAVLVLGGAGTAVQTLVAQSYGARRY
                     RRAAQATWTALWAMLCALPLFVAVGAAGRLLFAPFGFDPEIERLARAFWFPRVGGAAA
                     GAAVWAMMGFFNGTGRPRLTLLITVVTTVAN"
     gene            634..1650
                     /locus_tag="VM713_10130"
     CDS             634..1650
                     /locus_tag="VM713_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HVH84662.1"
                     /translation="MPTRSSAAGARTRRAPARRRGRLAPGGKARGLDAAEVVASLEDP
                     ALAPLTALVRTAGGAPIGAYREPLGGRALLLASLPLAALQPTPFQRDLSPTHAKRLAQ
                     KIDETQSFLDPVIVVRGEDGRLWTPNGRHRLAAAKVLGMKQITALISPDEALAYRILA
                     LNTEKAHNLKDRSLEVIRMARSLARSRGRDPETQFAAEFEAPELLTLGVVYESSPRFA
                     GGAYSAFLKKVDRFSDRGLTVSLRAREDYAARLKEIDARVKSLVAALQARGFKSPYLR
                     NYVVARINPVRFHKAKKGVTAPPMPLAQALTRMAAAARTFNSSAVSNSDLAWVAVGAG
                     SAES"
     gene            complement(1690..>1986)
                     /locus_tag="VM713_10135"
     CDS             complement(1690..>1986)
                     /locus_tag="VM713_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HVH84663.1"
                     /translation="LLERLHRRGVPVVAVFLSGRPLWIEAELAASDSFVAAWLPGAEG
                     GGVADVLFRTRDGRVAHDFRGRLPLSWPRAPVTGGAGTGPLFPYGYGLRYAPGH"
BASE COUNT          319 a          747 c          680 g          240 t
ORIGIN      
        1 cattcgcgac cgtcgtcacc acggtgatca gcaacgtgag gcgcgggcgg ccggtgccgt
       61 tgaagaatcc catcatcgcc cagaccgcgg ctcccgccgc cgcaccgccc acccgcggaa
      121 accagaaagc gcgggcgagg cgctcgatct ccggatcgaa gccgaagggc gcgaagagga
      181 gcctgcccgc cgccccgacg gccacgaaca gcggcagcgc gcacagcatc gcccacagcg
      241 cagtccaggt ggcctgggcg gcgcggcggt aacgccgcgc gccgtacgac tgtgcgacca
      301 gggtctgcac cgcggtgccc gcgcctccca acaccagcac agcaacgacg accagccact
      361 gcaccgcgcc gactgccgcc agcgccttgg tcgagatgcg cccgatgaac cacatgtcgg
      421 tgagattgag cacgatctgc acggcgctgt tggccatgag cggcagcgcc agcgccagca
      481 ctgctcggct gtcgacgtgc gcgcggccgg cggcgtcgag ccgctgcgcg ggcaagggtg
      541 gggcggtgct catcgtctgc agaggggaat tcatggcaac atcccgagga atcggtcatt
      601 ctcgcacatc gattcgcgcc gacgaggtcc tccatgccaa cacgcagctc cgccgcgggc
      661 gcgcgcacac gccgtgcgcc ggcgcgtcgc cgcggccgac tggctccggg cggcaaggcg
      721 cgcggcctcg acgccgccga ggtggttgcc tcactggagg atccggcgct cgcgccactc
      781 accgcactgg tgcgcaccgc gggcggcgcg ccgatcggcg cctaccgcga gccgctcggc
      841 ggccgcgcgc tgctgctcgc gagcctgccg ctcgcggcgc tgcaaccgac gcccttccag
      901 cgcgacctct cgccgacgca cgccaagcgc ctcgcgcaga agatcgacga gacccagtcg
      961 ttcctcgatc cggtcatcgt ggtgcgcggc gaggacggtc ggctgtggac tccgaacggc
     1021 cggcaccgcc tggcggccgc caaggtgctc ggcatgaagc agatcaccgc gctcatctct
     1081 cctgacgaag cgctcgccta ccgcatcctc gcactcaaca ccgagaaggc tcacaacctg
     1141 aaggatcgga gcctcgaggt gatccgtatg gcgcgcagcc tcgcccgcag ccgcggccgc
     1201 gaccccgaga cgcagttcgc cgcggagttc gaggcgccgg agctcttgac gctcggcgtc
     1261 gtctacgaga gctcgccgcg ctttgccggc ggcgcctaca gcgcgttcct gaagaaggtg
     1321 gaccgcttca gcgaccgcgg cctcaccgtg agcctgcgag cgcgcgagga ttatgcggcg
     1381 cggctcaagg agatcgacgc gcgcgtgaag agcctggtcg cggcgctgca ggcgcgcggc
     1441 ttcaagtccc cgtacctgcg caactacgtg gtcgcgcgca tcaatccggt gcgctttcac
     1501 aaggcgaaga aaggcgtcac ggctccgccg atgccgctcg ctcaggcgct gacccgtatg
     1561 gcagccgcgg cgcgcacctt caactcgagt gccgtgagca actccgatct cgcctgggtc
     1621 gcggtcggcg ccggctcggc ggagtcgtga agcggccggg ccgcgccgga gcgcggtcag
     1681 ccgcggcggc tagtgtcccg gcgcgtacct gaggccgtac ccgtacggaa acagcggccc
     1741 cgtgcctgcg ccccccgtaa ccggcgcccg cggccaggag aggggcaggc ggccgcgaaa
     1801 atcgtgcgcc acgcggccgt cgcgcgtgcg gaagagcaca tccgccacgc ccccaccctc
     1861 tgcgcccggg agccaggccg ccacgaagct gtcggaggcg gcgagctcgg cctcgatcca
     1921 caacggccgg cccgagagaa acaccgcgac caccggaacc ccccgacgat gaagccgctc
     1981 gagcag
//
LOCUS       DATLKL010000747         7304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_200691, whole genome shotgun sequence.
ACCESSION   DATLKL010000747 DATLKL010000000
VERSION     DATLKL010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200691"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1115)
                     /gene="fmt"
                     /locus_tag="VM713_10140"
     CDS             complement(192..1115)
                     /gene="fmt"
                     /locus_tag="VM713_10140"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HVH84664.1"
                     /translation="MAFAGTPEFALPALEALLKSHQVVGVMTQPDRPSGRGRHLHASP
                     VKALAAAHRLPLLQPESLREEAPRVELAAWKPEVLVVVAYGLLLPPAVLALPPLGCLN
                     IHASLLPRWRGAAPVQRAILAGDSITGITIMRMDDGLDTGPTLLKREIDIKADDTGGS
                     LHEVLAPLGAAALLAALEGLTAGTLEPQPQPREGITYAPKVQKSEARIDWRRDAAYLE
                     RQVRAFDPWPVAETTLDGQRLRIHSAVKMSVKDAKSAEYGSIILVQDGIMLVQCGQGQ
                     LGIRRVQRPGGRVLAVSEFAHNQSLAGRRFG"
     gene            complement(1135..1680)
                     /gene="def"
                     /locus_tag="VM713_10145"
     CDS             complement(1135..1680)
                     /gene="def"
                     /locus_tag="VM713_10145"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009682343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HVH84665.1"
                     /translation="MALRTILEFPDPRLRTRARPVTEFDAALATLIDDLFETMYAAPG
                     IGLAATQVDVHQRVIVVDLSGDRTGQLALVNPAIITREGEASTEEGCLSVPGIFDEVK
                     RAAKVRVRAQDRTGAVFERDYDDILAVAIQHEMDHLEGKLLVDYLSDLKRERIRKRLD
                     KERKERAARAPAPQGSAHRAY"
     gene            2007..3107
                     /locus_tag="VM713_10150"
     CDS             2007..3107
                     /locus_tag="VM713_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HVH84666.1"
                     /translation="MTATEAAVANAPPEAPAEAAPATSRVVAPAVNPTAPKSYTVKKG
                     DTLWGIATMFLKDPWLWPEVWVINPKIPNPHLIYPGDTLQLAFGANGRPQISVLSMGA
                     ARVESVRIDPRLRSTALDTAIPVIPYAAIQGFLSHPRLLTREQIKTAPYVVAFRDQHQ
                     VGGSGIEVYVRNLNAEQNARFAVMRVGDALRDPDDHALLGYEGIYTATALVERPGNPA
                     KALLTEPALETVAGDRLVTATDDAPPVTFKLRAPATDIHGRIMAIVGGTDLVGQFVVV
                     AINRGKRHGLDVGNVLAVDSSGEVVRDLYRGGRDIKSMGSGIETAFAPKVKLPNERTG
                     TLLVFKVYDRMSYGLIVGASDTIHLRDVVRNP"
     gene            3197..4288
                     /gene="dprA"
                     /locus_tag="VM713_10155"
     CDS             3197..4288
                     /gene="dprA"
                     /locus_tag="VM713_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HVH84667.1"
                     /translation="MDAARARATLARAPALTAQAAQALFAAAGGDLGEAARARTALRI
                     ELPPAARAYLSAPDERALAADLDWLAASGTAIVLCTDPEYPPLLRQMADAPAALYVQG
                     RVPLLAARQLAMVGSRSPTPAGRKTAREFAAWFARAGLTVTSGLATGIDAASHEGALQ
                     GGGATIAVFGSGLDRVYPDANRVLAERIRAHGALVSEFPPGTAPLARNFPRRNRIIAG
                     LALGTLVVEAARHSGSLSTAHHALEAGREVFAIPGSIHSPLSRGCHQLIRAGATLVEE
                     AEHVTAELGFSVSKERLTGLPVAVTQLRELDKEYEMLLDALGFEPATIDSLVARTELP
                     SESIASMLLILELEGRVAALPGGRYGRIP"
     gene            4291..4779
                     /locus_tag="VM713_10160"
     CDS             4291..4779
                     /locus_tag="VM713_10160"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HVH84668.1"
                     /translation="MTGSILDILIYVFDRYMLADAPEVPQREHLARDLERAGFTPAKV
                     ERALDWLTDLAFEHDRPPLPAAAPGAPAAGVRIFSDSELSRLSTECRGFLLTLERLGI
                     LTPQQREIVIERMLALDADEPDTEQLKWVVLMVLSSQPGQELAFERLDGLVSDDRAGT
                     PH"
     gene            4865..5398
                     /locus_tag="VM713_10165"
     CDS             4865..5398
                     /locus_tag="VM713_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HVH84669.1"
                     /translation="MADKLVIVESPAKAKTIKKYLGRDFDVLASYGHLRDLVPKEGAV
                     DPDNGYAMKYQVLEKNQRHVDAIARSLKKSSALYLATDPDREGEAIAWHLKEILRERG
                     DLNGKDVRRVVFYEITRNAIRAAVEQPRDLSLELVNAQQARRALDYLVASTSRRCCGK
                     RCGAGCRPAGCRARRCA"
     gene            5347..>7304
                     /gene="topA"
                     /locus_tag="VM713_10170"
     CDS             5347..>7304
                     /gene="topA"
                     /locus_tag="VM713_10170"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HVH84670.1"
                     /translation="MRRGLSAGRVQSPALRMICEREEEIAAFKAQEYWTVEGEGSFGG
                     QAFPLKLLEYRGGKVEQFTFTNETAAREAERAIGGAARGAGAEPVLTVLAIDRKQRRR
                     NPAPPFTTSTLQQEAARKLGFNARRTMRLAQQLYEGLDIGEGSVGLITYMRTDSVSLA
                     AEAVQEIRAVAARLYGQDEVAEEPRVYKTKSKNAQEAHEAIRPTSAAITPADVEGKIE
                     DDLYRLYALIWKRAVASQMSHALFDTVAVDLLAGPDGPERHLLRANGSTLVKPGYMSV
                     YQEDSDDPKTDEGDHVLPPMQEGERVALSALKAEQHFTEPPPRYSEASLVKALEEHGI
                     GRPSTYASIISTLQDRECVEMDSRRFVPTDIGKIVNRFLTHHFHRYVEYGFTAAMEDE
                     LDAVSRGEEAWTTPLAKFWKPFIHQVEKIDKTVTREQVAQARELGKDAASGKPITVRM
                     GRFGPFVQIGTKDDEEKPRFAGLRPGQKMDAITLADAMELFKLPRTLGATAQGETIVA
                     NVGRFGPYVKYGSKFVSLKEDDPYTVTLERALEVIRLKQEADAARLIRDFPEDGIQVL
                     NGRYGPYVTDKKKNAKIPKDRDPKMLSLEECRALLAAAPERGARFGRFGKRKQAAPPA
                     GAAAAAGGGEAPTTSAASAAPRGRKGAAT"
BASE COUNT         1199 a         2552 c         2445 g         1108 t
ORIGIN      
        1 tcatgagtcc ctcgagcgcc ggcacgagcc tcagatacca gcgcatcgtc ccgagcgtga
       61 tcgcgcgcac cgccgaatct gcagagtcgc cttgcgcgag tgccgcgtcg gcggagcggc
      121 cgttcatcgc aaccgcctca acccccctgg ccgccgcagc cagcgcgctt gcacccggcg
      181 cccaccgcgt cctagccaaa acgccggccc gcgaggctct ggttgtgggc gaattcggac
      241 accgcgagaa cccggccacc gggtctctgg actcgccgga tgccaagctg cccctgacca
      301 cactggacca gcatgatgcc gtcctgtact aggatgatgg aaccatattc tgctgatttc
      361 gcgtctttta ccgacatttt aaccgcagaa tgaatgcgaa ggcgctgtcc atcgagtgtg
      421 gtctccgcga ccggccaggg atcgaaggca cgaacctggc gctccaggta cgccgcatcc
      481 cgcctccagt cgatgcgagc ctcggacttc tggaccttgg gggcataagt gatgccctcg
      541 cgcggctgtg gctgtggctc gagcgtcccg gccgtcagcc cctcgagcgc cgcaagcagc
      601 gccgccgcgc cgaggggagc gaggacctcg tgcagcgatc caccggtgtc atcggctttg
      661 atgtcgatct cgcgcttgag gagcgtcggg ccagtgtcga gtccatcgtc catccgcatg
      721 atcgtgatgc cggtgatgga atcgcccgcg aggatcgcgc gctgcacggg cgcggcaccg
      781 cgccagcgcg gcaggagcga ggcatgaatg ttgaggcagc cgagaggtgg gagcgcgagc
      841 acggccggtg gcagcagcag cccgtacgcg accacgacca ggacctcggg cttccaggcg
      901 gcgagctcca cgcggggcgc ctcctcccgc aggctctcgg gctgaagcaa tggcaggcgg
      961 tgcgccgcgg cgagcgcttt caccggcgat gcatgcaggt gccggccccg gccgctcgga
     1021 cggtcgggct gtgtcattac gccgacgacc tggtgagact tgagcagcgc ctcgagcgcg
     1081 ggcagcgcaa attccggcgt ccccgcgaaa gccacgcgca aagccaggcc ccgtctagta
     1141 cgcgcggtgc gcgctgccct gaggcgccgg agcgcgcgcc gcgcgctcct tacgctcctt
     1201 gtcgagcctc ttgcggatgc gctcgcgctt gaggtcggag aggtaatcca cgagcagctt
     1261 cccctcgaga tgatccatct cgtgctggat ggccaccgcg aggatgtcgt cgtagtcgcg
     1321 ctcgaacacc gcgccggtgc ggtcctgagc ccgcacccga accttcgccg cgcgcttcac
     1381 ctcatcgaaa atccccggca cggacagaca cccctcctcg gtgctcgcct caccctcgcg
     1441 ggtgatgatc gcgggattga ccagcgcgag ctggccggtg cggtcgcccg agagatcgac
     1501 gacgatgacg cgctggtgaa cgtcgacctg ggtggccgcg agcccgatgc cgggagcggc
     1561 gtacatggtc tcgaacaggt cgtcgatgag cgtcgcgagc gcggcgtcga attcggtcac
     1621 cggacgtgcg cgcgtgcgca gccggggatc cgggaactcc aggatggttc ttaaggccat
     1681 gatttccgcg tatatggggg cgccgctgcc cgtgatccag cgcacgattt tgacattaat
     1741 tcatatgcca aatgggcggc atgccagtat aacaattaga atctcgcacg ctgcttgtgt
     1801 gccccacctc gtgcacagag gagtggagga tggtctctaa tcaagtactt ggagcccgct
     1861 taggcgcggg cctcctcagc ctgtcgctga tgctctccgg atgcgcgctg ttcaggcatt
     1921 cgcacccggc ccagcctgcg cccgccgccg ccacgcccgc ggcgagccca agcgagccca
     1981 tcgctgaggc cgccccggcg ccggagatga cggcgaccga ggccgcagtt gccaacgccc
     2041 ccccggaagc tcccgccgag gccgcgccgg ccacctcgcg cgttgtcgca cccgcggtga
     2101 atccgaccgc gcccaagagc tacaccgtca agaaaggcga cacgctctgg ggcattgcca
     2161 cgatgttcct caaggatccg tggctgtggc ccgaggtgtg ggtcatcaat ccgaagattc
     2221 cgaatccgca cctcatctat cccggcgaca cgctgcagct cgcctttggc gccaacggca
     2281 gaccgcagat atcggtcttg tcgatgggag ctgcgcgcgt cgagtccgtg cgcatcgacc
     2341 cgcgactgcg tagcaccgcg ctcgatactg cgatcccggt cattccgtac gccgcgatcc
     2401 agggattcct ctcgcatccg cgcctgctga cccgcgagca gatcaagacc gcgccgtacg
     2461 tggtcgcctt ccgcgaccag caccaggtgg gcggctccgg catcgaggtc tacgtgcgca
     2521 acctcaacgc cgagcagaat gcgcgcttcg ccgtgatgcg cgtcggcgac gcactgcgcg
     2581 atcccgacga ccacgcgctg ctcggctatg agggcattta cacggccacc gcgctcgtcg
     2641 agcgtccggg caaccccgcc aaggcgctgc tcaccgagcc ggcactcgag accgtggcgg
     2701 gcgaccgcct ggtgacggcg accgacgacg ccccgcccgt caccttcaag ctgcgcgcgc
     2761 ccgcgaccga catccatggc cgcatcatgg ccatcgtcgg cggcaccgat ctcgtcgggc
     2821 agttcgtggt cgtcgccatc aaccgcggca agagacacgg gctcgacgtc ggcaacgtgc
     2881 tcgccgtcga ttcttcgggc gaagtggtgc gcgatctgta ccgcggtggc cgcgacatca
     2941 agagcatggg cagcggcatc gagaccgcct tcgcgcccaa ggtgaagctc cccaacgagc
     3001 gcaccggcac gctgctggtc ttcaaggtat acgaccgcat gagctacggg ctcatcgtcg
     3061 gagcctcgga cacgatccac ctgcgtgacg tcgtccgtaa tccctgagcg cccactaagc
     3121 gcaaagctgg tctcttcgat gcgttaagcg ccgcgccgcc acgcggcgcg gcgcgcttgc
     3181 cttatgctcg cgcgccatgg acgcagcacg cgcacgcgcg acactcgccc gcgcccccgc
     3241 cctcaccgct caggccgcgc aggcgctgtt cgcggccgcc ggcggcgacc ttggggaggc
     3301 cgcgcgcgcg cgcaccgcgc tgcggatcga gctcccgccc gcggcgcgcg cttatttaag
     3361 cgctcccgat gagcgtgccc tcgccgccga tctcgactgg ctcgccgcga gcggtaccgc
     3421 gatcgtgctg tgtacggacc ccgagtaccc gcccctgctg cggcagatgg ccgatgcgcc
     3481 cgccgcgctc tacgtgcagg gacgggtgcc gctgctcgct gcgcggcagc tcgcgatggt
     3541 cggctcgcgc agccccaccc ccgccggccg caagacggcg cgcgagtttg ccgcgtggtt
     3601 cgcgcgcgcg ggactcacgg tcacgagtgg gctcgccacc ggcatcgacg ccgcgagtca
     3661 tgaaggcgcg ctccagggcg gcggtgcgac catcgccgtg ttcggcagcg gcctcgatcg
     3721 ggtctaccct gacgccaacc gcgtgctcgc tgagcgcatc cgcgcgcacg gcgcgctggt
     3781 atcggagttc ccgccgggca ccgcgccgct tgccaggaac tttccgcggc gcaatcgcat
     3841 catcgccgga ctcgcgctcg gcacgctggt ggtcgaggcc gcgcgccaca gcggctcgct
     3901 cagcaccgcg catcacgcgc tcgaggcggg ccgcgaggtg ttcgcgatcc cgggctcgat
     3961 ccacagtccg ctctcgcgtg gctgccacca actgatcagg gccggagcga cgctcgtcga
     4021 ggaagccgag catgttacgg cggagctggg attttcggtt tcgaaagaac gacttacggg
     4081 cctgccagtg gcggtgacgc agctgcgcga attggacaag gagtatgaaa tgctgttaga
     4141 tgcgctcggc ttcgagcccg cgaccatcga tagtctggtg gcgcgcaccg agctgccgag
     4201 cgagtcgatc gcctcgatgc tgttgatact cgagctcgag gggcgtgtgg cggctcttcc
     4261 gggcggccgg tatggccgca taccttaacc atgactggca gtattctcga catcctgatc
     4321 tacgtcttcg accggtacat gctcgccgat gcgccggagg tgccgcagcg cgagcacctg
     4381 gcgcgcgacc tcgagcgtgc cgggttcacg ccggcgaagg tcgaacgcgc gctcgactgg
     4441 ctgaccgacc tcgccttcga gcacgatcgc ccgccgctgc cggcggcggc gccgggtgca
     4501 ccggccgcgg gcgtgcggat cttctccgac agcgagctca gccgcctctc gaccgaatgc
     4561 cgcggcttcc tgctgacgct cgagcgcctc ggcatcctca cgccgcagca gcgcgagatc
     4621 gtcatcgagc ggatgctggc gctcgatgcc gacgagcccg acaccgagca gctcaagtgg
     4681 gtggtgctga tggtgctctc gagccagccc ggtcaggaac tcgcgttcga gcggctcgac
     4741 gggctggtgt ccgacgatcg cgcggggacg ccgcattgac gccgccgctc gcctgcaccc
     4801 cgtctggctc tggtccgcgg ctcgtccgcg gtttttcgtt ttaggcagag acgttttttt
     4861 ctaaatggca gacaagctgg tcatcgtcga gtcgccggcc aaggccaaga cgatcaagaa
     4921 gtatctcggc cgcgacttcg acgtgctcgc ctcctacggc catctgcgcg atctggtgcc
     4981 caaggaaggc gcagtcgatc ccgacaacgg ctacgcgatg aagtaccagg tgctggaaaa
     5041 gaaccagcgg cacgtggacg cgatcgcgcg cagcctgaag aaatcgagcg cgctctacct
     5101 cgcgaccgac ccggaccgcg agggcgaggc gatcgcctgg cacttgaagg agatcctgcg
     5161 cgagcgcggc gacctcaacg gcaaggatgt gcgccgggtg gtgttctacg agatcacgcg
     5221 caacgcgatc cgcgccgcgg tcgagcagcc gcgcgatctg tcgctcgagc tggtcaacgc
     5281 ccagcaggcg cgccgcgcgc tcgactacct ggtggcttca acctctcgcc gctgctgtgg
     5341 aaaaaggtgc ggcgcgggct gtcggccggc cgggtgcaga gcccggcgct gcgcatgatc
     5401 tgcgagcgcg aggaagagat cgcggcgttc aaggcgcagg agtactggac cgtcgagggc
     5461 gagggatcgt tcggcggcca ggccttcccg ctcaagctgc tcgagtatcg cggcggcaag
     5521 gtcgagcagt tcaccttcac caacgagacg gcggcgcgcg aggccgagcg cgccatcggc
     5581 ggcgccgcac gcggcgcggg tgcggagccg gtgctcacgg tgctggccat cgaccgcaag
     5641 cagcgccgcc gtaaccccgc tccgcccttc accacttcga ccctgcagca ggaggccgcg
     5701 cgcaagctcg gcttcaacgc acgccgcacc atgcgcctgg cgcagcagct gtacgaggga
     5761 ctcgatatcg gcgagggcag cgtcggactc atcacctaca tgcgcaccga ttcggtgtcg
     5821 ctggcagccg aggcggtgca ggagatccgc gcagtcgccg cgcggctata cggccaggac
     5881 gaggtggcgg aggagccgcg cgtctacaag accaagtcga aaaacgcgca agaggcgcac
     5941 gaggcgatcc gccccacctc ggccgccatc acgccggccg acgtcgaggg caagatcgag
     6001 gacgatctct atcggctcta cgccctcatc tggaagcgcg cggtcgcctc gcagatgagt
     6061 cacgcgctgt tcgacacggt ggcggtggac ctgctcgccg ggcccgatgg gcctgagcgc
     6121 catctgctgc gtgccaacgg ctcgacgctc gtgaagcccg gctacatgtc ggtctaccag
     6181 gaagacagtg acgacccgaa gaccgacgag ggcgatcacg tgctgccgcc gatgcaggag
     6241 ggtgagcgcg tggcgctcag tgcgctcaag gccgagcagc atttcaccga gccgccgccg
     6301 cgctacagcg aggcctcgct ggtgaaggcc ctcgaggagc atggcatcgg ccgcccctcg
     6361 acctacgctt cgatcatctc gacactgcag gaccgcgagt gcgtcgagat ggacagccgc
     6421 cgtttcgtgc cgaccgacat cggcaagatc gtcaaccgtt tcctcaccca tcacttccac
     6481 cgttacgtcg agtacggctt cacggcggcg atggaggacg agctcgatgc ggtgtcccgc
     6541 ggcgaggagg cgtggacgac gccgctcgcc aagttctgga agcccttcat ccaccaggtc
     6601 gagaagatcg ataagaccgt gacgcgcgag caggtcgcgc aggcgcgcga gctcggcaag
     6661 gacgccgcca gcggcaagcc gatcacggtg cgcatggggc gcttcggccc gttcgtgcag
     6721 atcggcacca aggacgacga ggagaagccg cgctttgcgg gcctgcggcc cggccagaag
     6781 atggacgcga tcacgctcgc ggatgcgatg gagctcttca agctgccgcg caccctcggc
     6841 gctaccgcgc agggcgagac catcgtcgcc aacgtcggcc gcttcggccc gtacgtgaag
     6901 tacggcagca agttcgtctc gctcaaggag gacgatcctt acacggtgac gctcgagcgc
     6961 gcgctggaag tcatccgcct gaagcaggag gcggacgccg cgcgtctcat ccgcgatttc
     7021 cccgaggacg gcatccaggt gctgaacggc cgctacggcc cgtacgtgac cgacaagaag
     7081 aagaacgcca agatcccgaa ggatcgcgac ccgaagatgc tgtcactgga agagtgccgg
     7141 gcgctcctcg ccgccgctcc ggagcgcggc gcacggttcg ggcgcttcgg caagcgcaag
     7201 caggctgcgc cgcccgccgg tgcagccgcc gcggcgggcg ggggcgaagc tcccaccacg
     7261 agcgcagcgt ccgccgcccc caggggccgc aagggcgctg cgac
//
LOCUS       DATLKL010000748         2184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_211988, whole genome shotgun sequence.
ACCESSION   DATLKL010000748 DATLKL010000000
VERSION     DATLKL010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211988"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VM713_10175"
     CDS             complement(<1..495)
                     /locus_tag="VM713_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVH84671.1"
                     /translation="MMQEVYDPAAVERTAQQYWRERRAFEVREDPAKAKFYCLSMLPY
                     PSGRLHMGHVRNYTIGDVLARYLRMQGYNVLQPMGWDAFGLPAENAAISNGVPPAKWT
                     RDNIAHMRAQLQALGFAIDWQREVATCDPDYYRWNQWLFLRMREQGLAYRKSGLVNWD
                     PVDQT"
     gene            complement(587..2110)
                     /locus_tag="VM713_10180"
     CDS             complement(587..2110)
                     /locus_tag="VM713_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HVH84672.1"
                     /translation="MNVKAESILVPPLRRALAVLALAVALAACSRDTSAQQTHPAPAP
                     ASMPAPALAVAADSGAADPALRALPDFTTLVEKYGPAVVNVEVVEKAQQAGGQGLSPN
                     DPFYDFFRRFGIPAPGEGPRANQPPLRGAGSGFIVSPDGYILTNTHVVADAETVTVRL
                     TDRREFQAKVIGADERTDVAVIKISANNLPIVRLGDPSRIKPGQWVLAIGSPFGFENT
                     VTAGIISATSRSLPSDNYVPFIQTDVAVNPGNSGGPLFNMAGEVIGINSQIFSRTGGF
                     MGLSFAIPIDVARNVEEQLIKTGRVVRGRIGVTIQDLNAQLAESFGLDRPRGALVSSV
                     EKDGPAAHAGIQPGDVILSVGGHPIEHYGELSGAIAAMKPGSDTTLSVWRGGKQLNVS
                     VAVAELREQQQTASARSRSQGGGGAKTPSVFGLTVRPLNPQEKQQAGTTGSLVVEEVS
                     GPAAAAEVQPGDIILGVNGKRVHTDQELQEAAKSAGKSVALLIQREDAQIFVPLRLP"
BASE COUNT          328 a          734 c          758 g          364 t
ORIGIN      
        1 cgtctgatcg accggatccc agttgacgag tccgctcttg cggtacgcga gcccctgttc
       61 gcgcatgcgc aggaacagcc actggttcca gcggtaatag tccgggtcgc aggtggccac
      121 ctcgcgctgc cagtcgatgg cgaagccgag cgcctgcagc tgcgcgcgca tgtgcgcgat
      181 gttgtcgcgc gtccatttgg cgggcggtac gccgttgctg atggcggcgt tctcggcggg
      241 cagcccaaag gcgtcccagc ccatcggctg cagcacgttg tagccctgca tgcgcaggta
      301 ccgcgcgagc acgtcgccga tcgtgtagtt gcgcacgtgg cccatgtgca gcctgcccga
      361 ggggtacggc agcatcgaca gacaatagaa cttcgctttg gcgggatcct cgcgcacttc
      421 gaaggcgcgc cgctcccgcc agtactgctg cgcggtgcgc tcgacagccg ccggatcgta
      481 gacttcctgc atcatgttcc catcggggcg gccggcaaaa accgcgcttt ttgccggcgg
      541 aaagcgaaat agtgtccggc gaaatcggcg cagccgattt tgcccattaa ggcaaccgca
      601 gcggcacgaa gatctgggcg tcctcgcgct ggatcagcag cgccacgctc ttaccggcgc
      661 tcttggccgc ctcctgcagc tcctgatccg tgtgcacgcg cttgccgttc accccgagga
      721 tgatgtcgcc aggctgcacc tcggcggcgg ccgcgggacc gctgacctcc tcgacgacga
      781 ggctgccggt cgtgccggcc tgctgcttct cctggggatt gagcggccgg acggtgagcc
      841 cgaagacgct cggcgtcttg gctccgccgc cgccctgcga acggctgcgg gccgaagccg
      901 tctgctgctg ctcgcggagc tcggcaacag ccacgctcac gttcagctgc ttgccgccac
      961 gccacaccga gagtgtcgta tcgctcccgg gcttcatcgc cgcgatcgcc cccgagagct
     1021 cgccgtagtg ctcgatcggg tggccgccga cggaaaggat cacatcgccg ggctggatgc
     1081 cggcgtgcgc cgccgggcca tccttctcga ccgaggagac cagtgcgcca cgcggccggt
     1141 cgagcccgaa ggactcggcg agttgcgcgt tgagatcctg gatggtgacg ccgatgcgcc
     1201 cgcgcaccac gcggccggtc ttgatcagct gctcctcgac gttgcgcgcg acgtcgatcg
     1261 ggatcgcgaa cgagaggccc atgaacccgc cggtgcggct gaagatctgt gagttgatgc
     1321 cgatcacctc gcccgccatg ttgaagagcg gaccgcccga gttgccggga ttcacagcca
     1381 cgtcggtctg gatgaacggc acgtagttgt cgctcggcag cgagcgcgac gtggcgctga
     1441 tgatgccggc ggtgacggtg ttctcgaagc cgaatggcga gccgatggcg agcacccact
     1501 gccccggctt gatgcgcgag ggatcgccga gcctgacgat cggcagattg ttggcgctga
     1561 tcttgatcac tgccacgtcg gtgcgctcat cggcgccgat gaccttggcc tggaactcgc
     1621 gccggtcggt gagccgtacg gtcacggtct cggcatccgc aaccacgtgg gtattggtga
     1681 ggatgtagcc gtccgggctc acgatgaagc ccgagcccgc gccgcgcagc ggcggctggt
     1741 tggcgcgcgg tccctcgccg ggcgccggaa tgccgaagcg gcggaagaaa tcgtagaagg
     1801 gatcgttcgg cgagagcccc tggccgccag cctgctgggc cttctcgacg acttcgacgt
     1861 tcacgaccgc gggtccgtat ttttcgacca gcgtcgtgaa gtccgggagg gcgcgcagcg
     1921 cggggtcggc cgcgccggag tcggcagcta ccgcgagcgc aggtgcgggc atgctggccg
     1981 gggcgggcgc cgggtgcgtc tgctgggcac tggtatcgcg cgagcaggcc gcgagcgcca
     2041 ccgccagcgc cagcacggcg agcgcccggc gcagcggcgg aacgaggatc gattcggctt
     2101 tcacattcat gtcgggcgcc ttcaggcagc tgcgtcggcc gacccggaac gggtccgcca
     2161 cagcctatca ttccgcaccc atag
//
LOCUS       DATLKL010000749         2132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_87977, whole genome shotgun sequence.
ACCESSION   DATLKL010000749 DATLKL010000000
VERSION     DATLKL010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87977"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VM713_10185"
     CDS             <1..597
                     /locus_tag="VM713_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HVH84673.1"
                     /translation="QLAQEEFGARPIGDGPWLKFTNPLTGTDIVIKDVIADNFLQQVL
                     LRPEEYDVIATLNLNGDYVSDALAAQVGGIGIAPGGNIGDGLAVFEATHGTAPGFAGK
                     DRVNPGSLILSAEMMLRYLGWREAADLIIKGVTHTIANKELTYDLARLREAIKAPKRE
                     LAARKNVDEDLQRLIPGAKLMSTSGFATAVIRHMDDVT"
     gene            707..1261
                     /locus_tag="VM713_10190"
     CDS             707..1261
                     /locus_tag="VM713_10190"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVH84674.1"
                     /translation="MPSFGASAPRLILVGLAPGMHGANRTGRPFTGDYAGILLYRTLY
                     EQGLASAPQSASASDDLRLIDTRIVNSVKCVPPGNKPLPAEIRCCNDYLQEELEQLSG
                     ARVYLALGRVAHDAVLMARGLTRGRFPFAHGREHALDEARWLIDSYHCSRYNTQTRRL
                     TPDMFREVMARACERAGLKNVGSS"
     gene            1264..>2132
                     /gene="uvrC"
                     /locus_tag="VM713_10195"
     CDS             1264..>2132
                     /gene="uvrC"
                     /locus_tag="VM713_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HVH84675.1"
                     /translation="MFDHKEYIAALPRRPGVYRMFGADHELLYVGKARSLRDRVGTYF
                     AAGNVNPKVQALVQQITAIEVTVTNSETEALLLEYNLIKAHKPRFNVVLRDDKSFPYI
                     HLDARHEFPRLVFYRGPRSAPGRYFGPFPSAGAVRDTLNQLQKLFRIRNCRDSFFANR
                     SRPCLQHQIGRCSAPCVGLITREAYAQDIAAAVKVLEGRSGEVNAELQSRMEEAGARL
                     EFERAAEIRDQLGALKRIQAQQVVTAEGARDADVFAIAGDAGEYSVSVMLVRGGRNLG
                     TNSYFPRAVLAEP"
BASE COUNT          357 a          741 c          713 g          321 t
ORIGIN      
        1 cagctcgcgc aggaggaatt cggcgcgcgc ccgatcggcg acggtccgtg gctcaaattt
       61 accaacccgc tcaccggcac cgacatcgtc atcaaggacg taatcgccga caacttcctg
      121 cagcaggtgc tgctgcggcc ggaggagtac gacgtcatcg cgacgctgaa tctcaacggc
      181 gattacgtct cggatgcgct tgccgcccag gtgggcggca tcggcatcgc cccggggggc
      241 aacatcggcg acgggctcgc ggtgttcgag gcgacgcacg gcacggcgcc gggcttcgcc
      301 ggcaaggacc gcgtcaaccc aggctcgctc atcctctcgg cggagatgat gctgcgctat
      361 ctcggctggc gcgaggccgc cgatctcatc atcaaaggcg tgactcacac gatcgcgaac
      421 aaggagctga cctacgacct ggccaggctg cgcgaggcca tcaaggcacc gaagcgcgag
      481 ctcgcggcgc gcaagaacgt cgacgaggat ctgcagcggc tcattccggg cgcgaagctc
      541 atgagcacct ccggtttcgc gactgcggtc atccgccaca tggatgacgt cacttaagcg
      601 ccagaggcgc agcgccgcgt acgatcccga ctgcgcgcgc tgtccgcgcc tcgcacggta
      661 cctcgaggca acgcggcgcc gccacgcgga ctactgggcg cggcccgtgc cttcgttcgg
      721 ggcgagcgca ccgcgcctca tcctcgtcgg gctcgcgccc ggcatgcacg gcgccaaccg
      781 caccgggcgg cccttcacgg gcgattacgc cgggatcctg ctctaccgga cgctctacga
      841 gcaggggctc gcgagcgcgc cgcagtcggc atcggcgagc gatgatctgc ggctcatcga
      901 tacgcgcatc gtcaactccg tcaagtgcgt gccgccaggg aacaagccgc tgcccgcgga
      961 gatccgttgc tgcaacgact atttgcagga ggagctcgag cagctatccg gcgcgcgcgt
     1021 gtatctcgcc ctcgggcgcg tcgcccacga cgccgtgctc atggcgcgcg gcctcacgcg
     1081 cggacgcttc ccgttcgcgc acgggcgcga gcacgcgctc gatgaggcaa ggtggctcat
     1141 cgactcgtat cattgcagcc gctacaacac ccagacccgg cggctgacgc cggacatgtt
     1201 ccgcgaggtg atggcgcgtg cctgcgagcg cgcgggcctc aagaatgtgg gttcgagctg
     1261 accgtgttcg atcataagga atacatagcg gcgctgccgc gccgcccggg cgtgtaccgc
     1321 atgttcgggg cggaccatga gctcctgtac gtgggcaagg cgcgcagcct gcgggaccgg
     1381 gtgggcacct acttcgccgc cggcaacgtc aacccgaagg tccaggcgct ggtacagcag
     1441 atcacggcca tcgaggtcac ggtcaccaac tccgagaccg aggcgctgct gctcgagtac
     1501 aacctcatca aggcgcacaa gccgcgcttc aacgtcgtgc tgcgcgacga caagagcttc
     1561 ccctacattc acctcgacgc gcgccacgag ttcccgcgcc tcgtgttcta ccgcggcccg
     1621 cgcagtgcgc cagggcgcta cttcggaccc tttccgagtg ccggcgcagt gcgcgatacg
     1681 ctcaaccagc tgcagaagct gttccgcatc cgcaactgcc gcgacagctt cttcgccaac
     1741 cgcagccgtc cctgtctgca gcaccagatc ggccgctgct cggcgccgtg cgtggggctc
     1801 atcacgcgcg aggcctacgc gcaggacatc gccgccgcgg tgaaggtgct cgaggggcgg
     1861 agcggtgagg tcaacgcgga gctgcagtcc cgcatggagg aggcgggcgc gcgactcgag
     1921 ttcgagcggg ccgcggagat ccgcgatcag ctcggcgcgc tcaagcgtat ccaggcacag
     1981 caggtggtga ccgccgaggg cgcgcgcgat gccgatgtgt tcgcgatcgc cggcgatgcc
     2041 ggggagtact cggtgagtgt catgctggta cggggcggtc gcaatctcgg caccaacagc
     2101 tacttcccgc gtgcggtgct cgccgagccg ca
//
LOCUS       DATLKL010000750         1628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_31623, whole genome shotgun sequence.
ACCESSION   DATLKL010000750 DATLKL010000000
VERSION     DATLKL010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31623"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..>1628
                     /locus_tag="VM713_10200"
     CDS             133..>1628
                     /locus_tag="VM713_10200"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruA"
                     /protein_id="HVH84676.1"
                     /translation="MTPLTPRQQLAAADHLAWIRARSAEAGVSRYRLAKEVCERLGWK
                     DRRGRLQEMACRKHLIELERRGEIALPAARCRPPQRRPAEACSTTPQVGGSLADLGAV
                     TVQPIRGGTAQSQIWEAMMAAHHPCGRAPLCGGQIRYLICTERHGVLGGLAVSAAAWR
                     LRARDQWLGWDDAKRAEHLAGIVCNSRFLILPTVQVDHLASHVLGQLTRRIVGDWQEK
                     YGIKPWLMETYVEAARTGTAYRAVNWIEVGMTAGRGRQDTTGKARLPQKRVFLYPLSR
                     AMLKRLCPARPAAEPGWVRREFGGAKLGDRRLDRRLLELAVAFFAQPLANITQACGSA
                     ARTKAAYRFFDHRETTMDAVLEPHHQATVNRMRRESLVLAVQDSSSLNYTMHPQTQGI
                     GPIGSRRDGAQGLIVHNTAAFRPDGLPLGLLDIQCWARDARTFGKKHQRHTTPIAAKV
                     SYKWIRPLTSIRAAADQCPNTRIVVTADREGDIFEFLHEAHNAGLDVLV"
BASE COUNT          272 a          534 c          541 g          281 t
ORIGIN      
        1 caatcgcaac agctcgcgta cgcgtcgcat ggtcagcctc tgtgctggca tctgctcccc
       61 cctccaatcg gtcctgatca tctcccataa gtgcctctgg acacagcaaa ggaattgagt
      121 taggcagggg gcgtgacgcc ccttacgccg agacagcagc tcgcggctgc agatcatctg
      181 gcgtggatcc gcgcgcgcag cgcggaggct ggcgtttcgc gttatcggct ggccaaggag
      241 gtctgtgagc ggctggggtg gaaggatcgg cgcggtcgac tgcaggagat ggcctgccgc
      301 aagcatctga tcgagcttga gcggcgtggt gagatcgcgc ttccggcagc gcgttgccgg
      361 ccgccccagc ggcgccccgc ggaagcgtgt tcgaccactc cgcaggtcgg tggcagcttg
      421 gccgatctgg gcgcggtcac ggtacagccg attcgcggcg gcacggcgca gagccagatc
      481 tgggaggcga tgatggcggc ccaccacccg tgcggccgag cgccattgtg cggcgggcag
      541 atccgctatc tgatttgcac tgagcgccat ggcgtgctcg gcggcttggc ggtcagcgcc
      601 gccgcttggc gattgcgggc gcgcgatcag tggctgggct gggacgatgc caagcgcgcc
      661 gagcatctgg cggggattgt atgcaacagc cgctttctga tcctgcccac ggtgcaggtc
      721 gaccacctcg cctctcacgt tctgggtcag ctgacgcggc ggattgtcgg cgattggcag
      781 gaaaaatacg gcatcaagcc gtggctgatg gaaacctatg tcgaggccgc gcgcacgggc
      841 acggcctatc gcgccgtcaa ctggatcgaa gtcggcatga ccgctggccg cgggcgtcag
      901 gacaccacgg gcaaggcgcg cttgccgcaa aagcgggtct ttctttatcc gttgtcccgg
      961 gcgatgctca agcggctgtg cccggcccgg ccggcggccg agcccggctg ggtgcgtcgc
     1021 gagtttggcg gcgccaagct cggcgatcgc cggctggatc ggcgactgtt ggagctggcc
     1081 gtagccttct tcgcgcaacc cttggccaat atcacgcaag cctgcggctc ggcggcgcga
     1141 accaaagccg cttatcggtt ctttgatcac cgcgagacga ccatggatgc cgtgctcgaa
     1201 ccgcaccatc aggccaccgt caatcggatg cggcgcgaga gcttggtgct ggccgtgcag
     1261 gacagctcca gcctcaacta caccatgcat ccgcagaccc agggcatcgg cccgatcggc
     1321 agccgtcgcg atggcgcaca gggcttgatt gtccacaaca ccgcggcctt ccggcccgat
     1381 ggcctgcctc tgggcctgct cgatattcaa tgctgggcgc gcgatgctcg cacctttggc
     1441 aaaaagcatc agcgccacac caccccgatc gcggccaagg taagctacaa atggatccgg
     1501 ccgctgacat cgatccgcgc cgccgccgat caatgcccca atacccgtat cgtcgtcact
     1561 gccgaccgcg aaggcgacat cttcgagttc ctgcacgaag ctcacaacgc cgggctcgac
     1621 gtgctggt
//
LOCUS       DATLKL010000751         2656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_99491, whole genome shotgun sequence.
ACCESSION   DATLKL010000751 DATLKL010000000
VERSION     DATLKL010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99491"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VM713_10205"
     CDS             complement(<1..248)
                     /locus_tag="VM713_10205"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HVH84677.1"
                     /translation="MLLVALCGALPPSAWALRPVRVYEVDLDEQSPTAVQGAMRQALV
                     RATGRRQAADDPALAGVIADAAKYVKSYTSGPRGEPQVV"
     gene            396..1427
                     /gene="purM"
                     /locus_tag="VM713_10210"
     CDS             396..1427
                     /gene="purM"
                     /locus_tag="VM713_10210"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HVH84678.1"
                     /translation="MTERPGITYRDAGVDIDAGDELVERIKPLVQRAQRREVLAGIGG
                     FGALVELPAGYRQPVLVAGTDGVGTKLRLAIDLGRHDTIGIDLVAMCANDVVVQGAEP
                     LFFLDYYATGKLRVAIAEAVVRGIVEGCVQAGAALVGGETAVMPGMYHGEDYDLAGFC
                     VGVVEKEAIIDGTRTVAGDAIIGLASSGPHSNGYSLIRKLVSVAGATPATLLEGHALF
                     ERLLAPTRIYVKSLLALLRALPVHALAHITGGGLTDNIPRVLPAGLEAVLERSRWHRD
                     PVFGWLQRAGNIDAAEMYRTFNCGIGMVAVVPAEAAAVAVELLTAQGETATIIGEVRT
                     GGRGVYIRE"
     gene            1424..2089
                     /gene="purN"
                     /locus_tag="VM713_10215"
     CDS             1424..2089
                     /gene="purN"
                     /locus_tag="VM713_10215"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HVH84679.1"
                     /translation="MSPAKPLRLAILISGRGSNMEALASACESGALAARVAVVIADRA
                     DAAGLDAARARGLDTAALTHRGERGAFEQELGAALERHRPEAVALAGFMRVLSAEFVA
                     RYLGLMFNVHPSLLPKYRGLDTHRRVLAAGDAVHGASVHFVTPELDAGPVVLQSQVAV
                     EPGDTEATLAARVLVTEHVIYPRVLGWFADGRLAWRDGAAWLDGRRLTAPLLEDFRGA
                     AAP"
     gene            2070..>2656
                     /locus_tag="VM713_10220"
     CDS             2070..>2656
                     /locus_tag="VM713_10220"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HVH84680.1"
                     /translation="MARPHPEAAARGGAVWAHGALLILALAALPATGAGAEELEPYQA
                     SYEGIWRGLVVTTSDLKLEHTGETWTLSSISTPRGIGRLAAGIFPPRQVSVVRVTASG
                     VLPQSFKSLGGDHAKSTDLSYDWGAARVSGTLDGAKVDQPLTPGVQDDGSVQLALMVE
                     LLAGRTPSSFQMIDRSGTRKYQFAREGEATLQTPMG"
BASE COUNT          384 a          907 c          971 g          394 t
ORIGIN      
        1 acgacctgcg gctcgccgcg cggaccggag gtatagctct tcacgtactt tgcagcgtcg
       61 gcgatcacgc cagcgagcgc cggatcgtcg gcggcctggc gccgtccggt ggcgcgcacc
      121 agcgcctggc gcatggcgcc ctgcaccgcc gtaggggact gctcgtcgag gtcgacttcg
      181 tagacccgca ccggccgcag cgcccatgcc gacggcggca gtgcgccgca cagcgcgacg
      241 agcagcacga gggctccacg cacgcggcgg ccggcggcag ttgatcttga gtaggattgc
      301 acatcgtccg gatcgcacac gggggcggtg gacggaaggt acaatagcac acgcgccaca
      361 cacccgacgg cgccggtgcg gtgcgcgtaa atcttatgac tgagcgtcct ggcattacct
      421 atcgtgatgc cggcgtcgac atcgacgccg gtgacgagct ggtcgagcgc atcaagccgc
      481 tggtgcagcg cgcgcaacgc cgcgaagtgc tcgccggaat cggcggcttc ggcgcgctgg
      541 tcgagctgcc ggcgggctac cgccagccgg tgctggtagc gggcaccgac ggggtcggta
      601 ccaagctgcg cctcgcgatc gacttaggaa ggcatgacac catcggcatc gatctggtgg
      661 cgatgtgcgc caacgacgtg gtggtgcagg gagccgagcc gctgttcttc ctcgactact
      721 acgccaccgg gaagctgcgt gtcgcgatcg cggaggcggt agtacggggc atcgtcgagg
      781 ggtgcgtgca ggcaggtgcg gcgctggtgg gtggtgagac tgcggtaatg cccggcatgt
      841 accacggcga ggattatgac ctcgccggat tctgtgtcgg cgtggtcgag aaggaggcga
      901 tcatcgacgg cacgcgtacc gtggccggcg acgccatcat cggcctcgcc tcctccggac
      961 cgcattccaa cgggtactcc ctgatccgta aactcgtgtc ggtggcgggc gccactcccg
     1021 caacgctgct cgagggccac gcgctgttcg agcggctgct ggcgccgacg cgcatctacg
     1081 tgaagtcgtt gctggcattg ctgcgcgcac tgccggtgca cgcgctggca cacatcaccg
     1141 gcgggggact cacggacaat atcccgcggg tgctgcccgc ggggctggag gccgtgctcg
     1201 agcgcagccg ctggcaccgc gatccggtgt tcggctggct gcagcgtgcc ggcaacatcg
     1261 acgcggccga gatgtatcgc accttcaact gcggcatcgg catggtggcg gtagtgccgg
     1321 cggaagcggc cgccgtggcg gtcgaactgc tcaccgccca gggcgagacc gccaccatca
     1381 tcggcgaggt gcgcacgggc gggcgcggcg tgtacatccg ggaatgagcc ccgccaagcc
     1441 gctcaggctc gccatactga tctccgggcg cggctccaac atggaggcgc tggcgagcgc
     1501 ctgcgagagc ggtgcgctcg cagcgcgcgt cgcggtcgtg atcgccgatc gtgctgatgc
     1561 tgccgggctc gacgcggcgc gcgcgcgcgg cctcgacacc gcagccctca cgcaccgcgg
     1621 cgagcggggc gccttcgagc aggagctcgg tgccgcgctc gagcggcacc ggcccgaagc
     1681 agtcgcgctg gccggcttca tgcgcgtgct gtcggcggag ttcgtggcgc gctaccttgg
     1741 gctcatgttc aacgttcatc cctcgctcct gccgaagtac cgcgggctcg acacgcaccg
     1801 ccgcgtactc gctgcgggcg atgccgtcca cggcgcgagc gtccacttcg tgacgccgga
     1861 gctcgatgcc ggcccggtcg tgctgcagtc gcaagtcgcg gtcgagccgg gcgacaccga
     1921 ggcaaccctc gccgcgcgcg tgctcgtcac cgagcacgtg atctatcccc gtgtcctcgg
     1981 ctggttcgcc gacgggcggc tcgcctggcg cgacggcgcc gcgtggctcg atgggcggcg
     2041 gctcacggcg ccgctgctgg aggacttccg tggcgcggcc gcaccctgaa gcggctgcgc
     2101 gtggcggggc ggtgtgggcg catggcgcgc tgttgattct cgcgctggcg gccctgccag
     2161 cgacaggcgc cggcgccgag gagctcgagc cctaccaggc gagctacgag gggatctggc
     2221 gcggcctggt ggtcacgacc tcggacctca agctcgagca caccggcgag acctggacct
     2281 tgagctccat cagcacgccg cgcggcatcg ggcgcctggc tgcggggatt ttcccgccac
     2341 gccaggtcag cgtcgtgcgc gtgacggcct ccggagtact gccgcagagc ttcaagtcgc
     2401 tcggcggcga ccacgcaaag tccaccgatc tgagctacga ctggggcgcc gctcgggtca
     2461 gcggcacgct cgacggcgcc aaggtggacc agccgctcac gccgggcgtg caggacgatg
     2521 gctcggtgca gctcgccctg atggtcgagc tgctcgcggg gcgcacgccg agcagcttcc
     2581 agatgatcga caggagcggc acgcgcaaat accagttcgc ccgcgagggc gaggccacgc
     2641 tgcagacgcc catggg
//
LOCUS       DATLKL010000752         2102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_54154, whole genome shotgun sequence.
ACCESSION   DATLKL010000752 DATLKL010000000
VERSION     DATLKL010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54154"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VM713_10225"
     CDS             <1..1364
                     /locus_tag="VM713_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVH84681.1"
                     /translation="LGRRVRSADGRTVNIIGAGLSGALLAVLLARRGRAVALHERRPD
                     PRQRQPERGRSINLALAARGIRALERARVMERVWPLAIAMRGRMVHERSGVASLQAYG
                     QREHEVIYSVGRAELNRVLIEEALRHANVTVHFNQQCTGVDASRNRLRLRDAGTAREY
                     ELELAPAIATDGAGSAVRASLAAAGRVTVSEQWLDHDYKELTVPPRDGAPALERHALH
                     IWPRGGFMLIALPNTDASFTATVFLARAGEAGFAALDSGSAVRQFFAREFPDAVPLLP
                     RLVEEFAQHPQGQLGTVHTTPWRVGGQLLLLGDAAHAIVPFHGQGMNAAFEDCRVLDE
                     LLDRHDDWETLFEEFERARRPNTEAIAQMALENYVEMRDTVLDTRFVRLKSLAMALER
                     RFPDRFIPRYSMVMFHPEIPYAEALRRGMLQAQLLDELDRAGGGDAKLAERLIRERLP
                     PVV"
     gene            1458..1844
                     /locus_tag="VM713_10230"
     CDS             1458..1844
                     /locus_tag="VM713_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVH84682.1"
                     /translation="MRASSPPGQVFALDLAGLAAPNVTVWSAWLCGRIAGVGALKDLG
                     DGTGEVKSMRTHPEFLRQGVGAALLERIISAARARGMARLSLETGSGPPFEPALALYR
                     NRGFRDGEAFADYLRTDFNQFLHLPL"
     gene            1871..>2102
                     /locus_tag="VM713_10235"
     CDS             1871..>2102
                     /locus_tag="VM713_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84683.1"
                     /translation="MKELIGRLIAVLFVGSFCIVGPLCLAVALGSALQRAALIYSGLH
                     AEGTVIAKRQTGSSRITYAPVFQFTAIDGRTYV"
BASE COUNT          314 a          701 c          742 g          345 t
ORIGIN      
        1 ctttggggag gcgcgtgagg agcgcggacg gccgcacggt gaatattatc ggcgccgggc
       61 tctcgggcgc cctgctcgcc gtgctgctgg cgcggcgcgg ccgcgcggtg gcgctccatg
      121 agcgccgccc ggatccgcgc cagaggcaac ccgagcgcgg ccgctcgatc aacctggcgc
      181 tcgccgcgcg cggcatccgc gcgctcgagc gcgccagggt catggagagg gtgtggccgc
      241 tcgcaatcgc gatgcgcggg cgcatggtgc acgagcgctc gggagtggct tcgctgcagg
      301 cctacgggca gcgcgagcat gaggtcatct actcggtggg acgcgcggag ttgaaccgcg
      361 tgctgatcga ggaggcgctg cggcatgcga acgtgaccgt gcacttcaac cagcagtgca
      421 ccggggtgga tgccagccgc aaccggctgc ggctgcgcga tgctgggacc gccagggagt
      481 acgagctcga gctcgctccg gccatcgcca ccgacggggc gggctcagcc gtgcgcgcca
      541 gcctcgccgc cgccggtcgc gtcacggtaa gcgagcagtg gctcgaccac gactacaagg
      601 agctgaccgt tccgccccgc gacggcgcgc ccgcgctcga gcggcacgcg ctgcacatct
      661 ggccgcgcgg cggcttcatg ctgatcgcgc tgcccaacac ggacgcgagc ttcaccgcga
      721 ccgtgttcct ggcgcgcgcg ggagaggcgg gttttgccgc gctcgacagc ggcagcgcag
      781 tcaggcagtt tttcgcccgg gagttccctg acgccgtgcc gctgctgccg cgtctcgtcg
      841 aggagttcgc gcagcacccc cagggccagc tcggcaccgt gcacaccact ccctggcgtg
      901 taggcgggca gctcctgctg ctcggcgacg ccgcgcacgc catcgtgccg tttcacggcc
      961 agggcatgaa cgccgctttc gaggactgcc gggtgctcga cgagctgctc gaccggcatg
     1021 atgactggga gacgctgttc gaggagttcg agcgcgcgcg ccgtcccaac accgaggcga
     1081 tcgcgcagat ggcgctcgag aactatgtcg agatgcgcga cacggtgctc gatacgcgtt
     1141 tcgtgcgcct gaagagcctc gccatggcgc tggaacggcg cttccccgac cgcttcatcc
     1201 cgcgctattc catggtgatg tttcacccgg agatccccta cgcggaggcg ctgcgccgcg
     1261 gcatgctgca ggcgcagctg ctcgatgagc tcgaccgcgc aggcgggggg gacgcaaaac
     1321 tcgccgagcg cctgatccgc gagcggctgc caccggtcgt gtagggggcg tgcatcaact
     1381 ccgcggggct cgagattcgg gaggacgatt tggcgggcga gccgacccgc gagttactgc
     1441 ggctccattt gacggggatg cgcgccagct cgcctccggg ccaggtcttt gcactcgacc
     1501 tcgcaggcct cgcggcgccg aacgtcaccg tatggagcgc ctggctttgc gggcgcatcg
     1561 cgggcgtcgg cgccctgaag gatctcggcg atggcaccgg agaggtgaag tcgatgcgca
     1621 cacaccctga gtttcttcgt cagggtgtgg gagccgcgct gctcgagcgc atcatttctg
     1681 ctgcgcgggc acgcggcatg gcgcggctca gcctcgagac cggcagcgga ccgcctttcg
     1741 agcccgcgct cgcgttgtat cgcaaccgag gattccgcga cggcgaggca ttcgcagact
     1801 acctgcgcac tgacttcaat cagtttttgc atctcccgct ctgaaagctg gtatcgggac
     1861 aggttcagcg atgaaggaat tgatcggacg tctcatagca gtcctgtttg tcggaagctt
     1921 ctgcatcgtg ggaccactat gcctggcagt cgctctcggc tcggcgctgc aacgcgcagc
     1981 gttgatttac tccgggctgc acgcggaggg cacggtgatc gccaagcgac agaccggatc
     2041 gagccggatt acgtacgcgc ccgtgttcca atttacggcg atcgatgggc gcacctatgt
     2101 cg
//
LOCUS       DATLKL010000753         1695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_20246, whole genome shotgun sequence.
ACCESSION   DATLKL010000753 DATLKL010000000
VERSION     DATLKL010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20246"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /gene="ppk2"
                     /locus_tag="VM713_10240"
     CDS             <1..729
                     /gene="ppk2"
                     /locus_tag="VM713_10240"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HVH84684.1"
                     /translation="RRLQIQLVKLHRHVIARGHKVLVILEGRDTAGKDGTIKHIVEHL
                     SPREVRVVAPGKPSDREETEWYFQRFTAHLPAAQEIVLFNRSWYNRAGVEYVMKYCTR
                     AEHEAFLEAAPGFERMLVRSGIHLLKYYLDISCAEQARRLRARRHDPLKQWKLSPVDE
                     AAQQHWNDYSRARDQMLLRTHTRDAPWVVVRADDKHQARLNVIRDMLARVRAPDVARR
                     LARPDARVVREFSEPLLGSGWVAR"
     gene            789..1157
                     /locus_tag="VM713_10245"
     CDS             789..1157
                     /locus_tag="VM713_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84685.1"
                     /translation="MLVECLHHVQLAMPAGGEPRAREFYQGLLGIPEVPKPATMAARG
                     GCWFERGGLRVHLGVEADFRPARKAHPAFVVTGLSALAARLRAARHECREDTGLSSYD
                     RVFVDDPFGNRIELLEPRPA"
     gene            1154..>1695
                     /locus_tag="VM713_10250"
     CDS             1154..>1695
                     /locus_tag="VM713_10250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH84686.1"
                     /translation="MSEFRSVTPRFAVQALMFAAAAIVMLGNYYGYDSIGPVAEQLSR
                     ELHFSDTQIGTLNAIYSLPNVFLVVIGGVLVDRLTARRLVVATTAICLVGAAVTAVGA
                     QFPVMALGRLLYGIGSETLVVAVLVALAQWFAGRYLALLMAMNLSLARLGSYLADQSP
                     SFAGDLYARGWQPPLWLAFGL"
BASE COUNT          247 a          566 c          594 g          288 t
ORIGIN      
        1 cggcgactgc agatccagct ggtgaagctg caccgccacg tgatcgcccg cgggcacaag
       61 gtgctggtga tcctcgaggg gcgggatacc gccggcaagg acggcacgat caaacacatc
      121 gtcgagcacc tgagcccccg cgaggtgcgg gtcgtcgcac ccggcaagcc gagcgaccgc
      181 gaagaaaccg aatggtactt ccagcgcttc accgcgcatc tgccggccgc ccaggagatc
      241 gtgctgttca accgcagctg gtacaaccgc gccggcgtcg agtacgtcat gaagtactgt
      301 acgcgcgcgg agcacgaggc cttcctcgag gcggctcccg gctttgagcg gatgctggtg
      361 cgctcgggca tccacctcct caagtactac ctcgacatca gctgcgccga gcaggcccgg
      421 cggctgcgcg cgcggcgcca tgatccgctc aagcagtgga agctgagccc cgtggacgag
      481 gcggcacagc agcattggaa cgattacagc cgcgcccgtg atcagatgct gctgcgcact
      541 cacacgcgcg acgcgccgtg ggtggtggtg cgcgccgacg acaagcacca ggcgcggctc
      601 aacgtcattc gcgacatgct ggcgcgggta cgcgcgccgg acgtcgcacg cagactcgcg
      661 cgtcccgatg cgcgcgtggt gcgcgagttc agcgagcccc tgctcggctc gggatgggtg
      721 gcgcgctgag ggagcgccga gcgaatcgcg atccgcgggc ggcgtcattt gagtacaatg
      781 gcgcgccgat gttggtggaa tgtctgcatc acgttcagct ggccatgccc gccgggggcg
      841 agccgcgcgc gcgcgagttc tatcagggcc tgctcggcat ccccgaggtg ccgaagccgg
      901 cgaccatggc ggcgcgcggc ggctgctggt tcgagcgtgg gggactgcgc gtgcatctcg
      961 gcgtggaggc cgacttccgg ccggcgcgca aggcccatcc ggccttcgtc gtgaccggac
     1021 tgtctgcgct cgccgcgcgg ctgcgcgctg cacggcacga gtgtcgcgag gacaccgggc
     1081 tcagcagcta cgatcgcgtg ttcgtcgatg atccgttcgg caaccgtatc gagctgctcg
     1141 agccacggcc ggcatgagcg agtttcgttc cgtcacgccg cgattcgccg tgcaggcgct
     1201 gatgtttgcg gctgccgcga tcgtcatgct gggcaattac tacggctacg actcgatcgg
     1261 cccggtcgcc gagcagctct cacgcgagct gcacttctct gacacgcaga tcggcaccct
     1321 gaacgcgatc tacagcctcc ccaatgtctt cctggtagtg atcggcggtg tgctggtcga
     1381 tcggttgacc gcgcggcgcc tggtggtcgc gactacggcg atctgtctgg tgggagcggc
     1441 ggtcaccgcc gtcggtgcgc agttcccggt gatggcgctc ggcaggctcc tctacgggat
     1501 tggctcggag acgctggtgg tggccgtgct agtggcgctc gcgcagtggt tcgccggacg
     1561 ttatctggcg ctgctgatgg cgatgaacct gagcctggcg cgcctcggct cctatctggc
     1621 cgaccaatcc ccgtccttcg ccggcgacct ctacgcccgc ggctggcagc cgccgctgtg
     1681 gctcgcgttc ggtct
//
LOCUS       DATLKL010000754         2462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_54165, whole genome shotgun sequence.
ACCESSION   DATLKL010000754 DATLKL010000000
VERSION     DATLKL010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54165"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..2302
                     /locus_tag="VM713_10255"
     CDS             56..2302
                     /locus_tag="VM713_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVH84687.1"
                     /translation="MSAQDISDPPPEHSTARFEPYFRLVRSLLPRASSLAIFNDAGEF
                     LWSSETVTGPDLMNVVEDALISARSSEGGAGQLRTVAGDLPVYLCPLRDDAGGLLAII
                     ALVCRPNDGGDRRQDFSFAHSLLAPALECLRRDLIAYKNIDELNGTLAALNRDLELVL
                     SQGATDSPTPDGANDLQVLLEQTIEHLGASTGALLVPEKNLTLLRASGDATPDVRFMT
                     RAHRKLMSLAQTEAGPLIVNDVDVALTDTYPYRVLACALRSRGGRCLGMLALLRDEAS
                     ERFSQRDAHIAHIISRKALDIVESSYDALSGLYTRPAFERRLAAVVTDGKGAQPWSAL
                     YIDVDQLNAINEKAGMHVGDSVLGQLGELFRKRLPPGAFAARISGDRFAVLLPAQVED
                     AEQFAQALRAGAEALVPNPGESRPPVSISVGVALVASEDGELGHVLAAAESACKAAKD
                     RGRNRVEVYEANDTSIVRRFADISVAAQLRDAIDNDRLRLDAQLILPFAAAESARPHY
                     ELLLRMINDDGLTMGPDSFLAAAARYQLMPVIDRWVVNHVIEALQPRAGVLEGKALGF
                     TINFSGQSLDDDTFADFLIDSITESGLDPDLFCFELTENATVAHLPRAEELMRRLRKL
                     GCGVALDDFGTGLSSLSCIRRLPITMLKIDGSFVRDVVGDRRAESMVRAIAELARGMS
                     IITVAEYIETEAIGEHIAGLGVDYGQGFAIGRPIALAELLNELPLPSEAGAQRGPNDI
                     MWYAGATP"
BASE COUNT          377 a          885 c          814 g          386 t
ORIGIN      
        1 aggcaatcct gtcgtctggc cccgacacct cccacgtggc atccgacggc gattcatgag
       61 cgcgcaggac atcagcgatc ctccgcccga gcactccact gcccggttcg agccctattt
      121 ccgcctggtc cgctcgctcc tgccgcgcgc ctcgagcctc gcgattttca acgacgccgg
      181 cgagttcctc tggtcctcgg agaccgtgac gggacccgat ctcatgaacg tggtcgagga
      241 tgcgctcatc agtgcccgct cgagcgaggg tggcgcgggg cagctgcgca cggtcgccgg
      301 ggacctgccg gtctacctgt gcccgctgcg cgacgatgcc gggggactcc tcgcgatcat
      361 tgcgctcgtc tgccggccga acgacggcgg cgaccgcagg caggatttct ccttcgccca
      421 ttcactgctc gcgccggcgc tcgagtgcct gcggcgcgac ctgatcgctt acaaaaacat
      481 cgacgagctg aacggtacgc tcgccgcgct caaccgcgac ctcgagctgg tgctctcgca
      541 gggcgcgacc gattcgccca cacccgacgg cgccaacgac ctgcaggtgc tgctcgagca
      601 gacgatcgag cacctcggtg cgagcaccgg ggcattgctg gtgccggaga agaacctgac
      661 gctgctgcgt gcgagcggcg acgccacgcc cgacgtgcgc ttcatgaccc gggcacatcg
      721 caagctcatg tcgctcgcgc agacggaggc ggggcccctc attgtcaacg acgtcgacgt
      781 cgcgctcacc gacacctacc cataccgtgt gctcgcctgc gcgctgcgct cgcgcggcgg
      841 gcgctgcctc gggatgctgg cgctgctgcg cgacgaggcg agtgagcgct tcagccagcg
      901 cgacgcccac atcgcccaca tcatttcccg caaggcgctc gacatcgtcg agtcgagcta
      961 cgacgcgctg agcggcctgt acacccgtcc ggccttcgag cggcgcctgg ccgccgtggt
     1021 gaccgacggc aagggcgctc agccctggag cgcgctctac atcgacgtcg accagctgaa
     1081 cgccatcaac gagaaggccg gcatgcacgt cggcgattcc gtgctcgggc agctggggga
     1141 gctgtttcgc aagcgcctgc cacccggagc cttcgccgcg cgcatctcgg gcgaccggtt
     1201 cgcggtgctg ctgccggcgc aggtggagga tgccgagcag ttcgcccagg cgctgcgtgc
     1261 cggagcggag gcgctggtgc cgaatccggg cgagtcgcgc ccgccggtct cgatcagtgt
     1321 cggcgtcgcg ctcgttgcga gcgaggacgg tgagctcggc cacgtgctcg cggccgccga
     1381 gagcgcctgc aaggccgcca aggatcgcgg ccgtaaccgc gtcgaggtct acgaggcgaa
     1441 cgacaccagc atcgtgcggc gcttcgccga catcagcgtc gccgcgcagc tgcgcgatgc
     1501 gatcgacaac gaccggctgc ggctcgatgc gcagctcatc ctgccttttg ccgctgccga
     1561 gagcgcccgg ccgcactacg agctgctgct gcgcatgatc aacgacgacg gactcaccat
     1621 gggtccggac agcttcctcg ccgccgccgc gcgctaccag ctgatgccgg tcatcgaccg
     1681 ctgggtggtg aaccacgtga tcgaggccct gcagccccgg gccggtgtgc tcgagggcaa
     1741 ggcgctcggc ttcacgatca atttctccgg ccaatcgctc gatgacgata ccttcgccga
     1801 cttcctcatc gactcgatca ccgagagcgg cctggatccg gacctcttct gcttcgagct
     1861 caccgagaat gccaccgtcg cgcacctacc gcgcgccgag gagctgatgc gccgcctgcg
     1921 caagctcggc tgcggcgtgg cgctcgatga cttcggtacc ggcctctcct cgttgtcatg
     1981 catccggcgg ctgccgatca ccatgctgaa gatcgacggc agtttcgtgc gcgacgtcgt
     2041 cggggacagg cgcgccgagt ccatggtgcg cgccatcgcc gagctcgccc gcggcatgtc
     2101 gatcatcacc gtggccgagt acatcgagac cgaggccatc ggcgagcaca tcgccgggct
     2161 cggcgtcgac tatggccagg gctttgcgat cggcaggccg atcgcgctcg cggagctgct
     2221 gaacgagctg ccgctgccga gcgaggccgg ggcacaacgg ggaccgaacg acatcatgtg
     2281 gtacgcagga gcgacgccgt gaagggagtt tcgaggacat gaccagccgc tcacggacac
     2341 tgatcgcggt cgtcgccgga gccatcttcg gcttctgcgg cgcgctcgcg agcggcgtcc
     2401 tcgcggaacg ctccaccgag cccgcgcatg ccgcaccgcc cgccatcctc ccctgggagg
     2461 ag
//
LOCUS       DATLKL010000755         2044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_178272, whole genome shotgun sequence.
ACCESSION   DATLKL010000755 DATLKL010000000
VERSION     DATLKL010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178272"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VM713_10260"
     CDS             <1..509
                     /locus_tag="VM713_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVH84688.1"
                     /translation="IVAAVQGAAIGGGTTMLTHCDFVYAVESAKFQLPFINLALVPEF
                     GSSFSIPARVGHLRAAELYLLGEPFTAARAAELGLVTRVVPDADLLTTAIATAEKLAA
                     KPSGALLASKRLLKQPSIGQLNAAIKSESQEFMERVSGPEAKEALSAFLEKRPPNFNK
                     TKAPVAAK"
     gene            681..2003
                     /locus_tag="VM713_10265"
     CDS             681..2003
                     /locus_tag="VM713_10265"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVH84689.1"
                     /translation="MSVMLAETRADEATAVAEAPRRERKRVVIIGGGFAGIAAARALR
                     RADVDVILIDRRNHHIFQPLLYQVATAVLSPAEIAAPIRQLEVKQKNLSVLLAEVTGI
                     DVTSRMIEASAPGVGALKIVYDYLVVATGMRPSYFGHDEFAQYAPGLKNLSDAETIRA
                     KILGAFELAATTEDEAERARQMTFVLVGAGPSGVELAASLAQMVKVTLHGNFRHIDPA
                     QANIILLDAGKRVLPTFAESLSRRATRRLEKLGVKVLTGVKVETVDAQGVVAGGNRIP
                     SATVLWTAGVAASPIPKMLGTKTDRAGRALVDPFLKVVEAPGVFVVGDAASVMQKSEH
                     PVPGVAQAAIQEGHYVGRLIARELKGRKVKRPFSYFDKGNMAVVGKNYAVLERGWLRT
                     SGALTWLVWAFVHILALPQLQNRWRVQHQWLWSYFTGQRSSRLIPEPP"
BASE COUNT          375 a          639 c          688 g          342 t
ORIGIN      
        1 cgatcgtcgc ggccgtgcaa ggcgcggcga ttggcggtgg aacgacgatg ctgacgcact
       61 gcgatttcgt ctacgcggtc gagagcgcga aattccagct gcccttcatc aacctggccc
      121 tggtgccgga gttcggatcg agcttttcga taccggcgcg tgtgggccat ctccgggcgg
      181 cggaattgta cttgctggga gagcccttca cggccgcgcg ggcagctgag ctgggcctcg
      241 tgacccgcgt ggtgcccgac gccgacctgc tcacgaccgc catcgcaacg gcggagaagc
      301 tggcggcaaa accgagcggc gcgttgctgg cgagcaagcg gctcctcaag cagccttcga
      361 ttggccagct caatgcggcg atcaagagtg aaagccagga gttcatggag cgggtgagtg
      421 ggccagaggc caaggaagcg ctctcggctt tcctcgagaa gcgtccgccg aatttcaaca
      481 agacgaaggc gcccgtcgcg gccaaatagc ccgcgcccac agcaatcgat gggcgtcgcg
      541 acgccgcggc gcccagcgaa tcggctcctg gtgcgccggg atgccgtagc gaggccgaat
      601 ggccggaacg tagagagcat tcttcccgac agaagcttgc tgaatctgtc aatgacaaag
      661 agcacgaacg aggtgaacct atgagtgtga tgctggctga gacccgggct gacgaggcga
      721 ctgccgtcgc cgaggccccc cgccgagagc gcaagcgcgt cgtcatcatc ggcggcggct
      781 ttgcaggaat tgcagcggcg cgtgcgctcc ggcgcgccga tgtcgatgtc atcctgatcg
      841 accgccgcaa tcatcatatc tttcagccgc tgctctacca ggtggcgacg gcagtcctct
      901 cgcctgcgga gatcgccgcg ccgatccgtc agctcgaagt gaagcagaaa aacctcagcg
      961 tgctcctggc ggaagtcacc ggcatcgatg tgacttcccg catgatcgag gcgtctgcgc
     1021 ctggcgtcgg cgcgctcaaa atcgtctacg actacctggt ggtcgcgacc ggcatgcgtc
     1081 cgagctactt cgggcacgac gagttcgctc aatatgcgcc gggcctcaag aacctcagtg
     1141 acgctgagac gatccgagcc aaaatcctgg gcgcgttcga attggccgca acgaccgagg
     1201 atgaagccga acgcgcgcga cagatgacgt tcgtgctggt cggcgctggt ccctccggcg
     1261 tggagctcgc cgcgtctctc gctcagatgg tgaaggtcac gctgcacggc aacttccgcc
     1321 acatcgatcc ggcacaggcg aacatcattc tgctcgacgc aggcaagcgg gttctaccga
     1381 cctttgccga gtcgctgtcc cgcagggcga cccgacggct cgaaaagctc ggcgtgaaag
     1441 tcctgaccgg agtgaaggtc gagaccgtcg acgcgcaggg cgtggtcgcc ggcggaaacc
     1501 ggatccccag cgccacggtg ctctggaccg cgggggttgc ggcatcgccg atccccaaga
     1561 tgctcggcac caagaccgac cgcgcagggc gcgcgctggt tgatcccttc ctgaaggtcg
     1621 tggaggcgcc cggcgtcttc gtcgtgggcg atgcggcctc ggtcatgcaa aaaagcgaac
     1681 acccggtgcc gggcgtggcg caggcggcga tccaggaagg acactatgtc gggcggttga
     1741 tcgccaggga gctgaaggga cggaaggtca agcgcccgtt cagctatttc gacaagggca
     1801 atatggcggt cgtcggcaag aattacgcgg tgctggagcg cggctggctg cgcacgagcg
     1861 gcgccttgac ctggctggtg tgggcgttcg ttcacatcct cgccctgccg cagttgcaga
     1921 accgttggcg cgtgcagcac cagtggctct ggtcgtattt caccggccag cgcagctcgc
     1981 gcttgatccc tgagccgccg tgaatagcga atgggcaaaa ggtcgatcgc ccgccagttc
     2041 gctg
//
LOCUS       DATLKL010000756         1714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_65624, whole genome shotgun sequence.
ACCESSION   DATLKL010000756 DATLKL010000000
VERSION     DATLKL010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65624"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VM713_10270"
     CDS             <1..241
                     /locus_tag="VM713_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HVH84690.1"
                     /translation="IRRLHKAFLRYHDPDNWPLLREALRRMGRAELIGNGRHQLIPAY
                     QPTGTGGGAHPRPGARARPFRTQHTGLPRTPRRRP"
     gene            287..1477
                     /locus_tag="VM713_10275"
     CDS             287..1477
                     /locus_tag="VM713_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035444 - nickel cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux transporter"
                     /protein_id="HVH84691.1"
                     /translation="MTSFVTLLQQGNSWLFVPSAILLGALHGLEPGHSKTMMAAFIVA
                     IRGTLAQAVLLGLSATVSHTAVVWAVAPTGLYFGRHWNTETTEPYFQVASAVLIIAVA
                     VWMVWRTRRESTARADYAPLHDHHHDHHHDGHGHHHSHDRGHQDHGGAHQEAHRIDTG
                     HGVVRLAIFEDGVPPRFRLYGESGPAWPAEQVQIETERADGSRELFSFRRHGGYLESE
                     RTVPEPHEFVARLRLGPPAHSHDFDLEFVEGGHRHAIPGYRGLDVAAPGYQDPHELAH
                     ANDIRRRFADRNVTSGQIVVFGLTGGLIPCPASITVLLLCLQLKRLALGAVLVLCFSI
                     GLALTMVTTGAVAALGVQHAARRFSGFGELARKAPYFSGAVIMLVGFYVGYQGLHALA
                     VSRV"
     gene            1526..>1714
                     /locus_tag="VM713_10280"
     CDS             1526..>1714
                     /locus_tag="VM713_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84692.1"
                     /translation="MRLSSYRALVVFLLGCGIAVAATGPKEAAPATGVATPAASAAEP
                     PGTALVLEINGPIGPATSR"
BASE COUNT          239 a          612 c          599 g          264 t
ORIGIN      
        1 gatacgccgc ctgcacaagg cgttcctgcg ctatcacgac cccgacaact ggccgctgct
       61 gcgcgaggcg ctgcggcgca tggggcgcgc cgaactgatc ggtaacggtc gccaccagct
      121 gatccctgcc taccagccga cgggcacggg cggcggggcc cacccgcgcc cgggggcccg
      181 cgcccggccc ttccgcaccc agcacaccgg gctgccgcgc actccgcgcc ggcgacccta
      241 gccgggtata ctataccccc taccctatag tccgcggacg ggcccgatga cgagcttcgt
      301 gaccctgctg cagcagggca actcctggct gttcgtgccg agcgccatcc tgctcggcgc
      361 gctgcacggg ctcgagcctg ggcactcgaa gacgatgatg gccgccttca tcgtggcgat
      421 ccgcggcacg ctcgcgcagg cggtgctgct cgggctctcg gccacggtgt cgcacaccgc
      481 ggtcgtgtgg gccgttgcgc ccaccgggct gtacttcggc cgccactgga ataccgagac
      541 gaccgaaccg tactttcaag tcgcctcggc ggtgctgatc atcgcggtgg cggtgtggat
      601 ggtgtggcgt acgcggcgcg agagcacggc gcgcgccgac tatgcgccgc tgcacgacca
      661 ccatcacgat catcaccacg acggtcacgg ccatcaccac agtcatgatc gtggccacca
      721 ggatcacggc ggcgcgcatc aggaggcgca ccgcatcgac acgggccacg gcgtcgtgcg
      781 cctcgcgatc ttcgaggatg gcgtgccgcc gcggtttcgc ctctacgggg agagcggccc
      841 cgcatggccg gccgagcagg tgcagatcga gaccgagcgt gcggacggca gccgcgagct
      901 tttcagcttc cgccggcacg gcggctatct cgagtcggag aggacggtcc ccgagcccca
      961 cgagttcgtc gcgcgactgc gcctcgggcc ccccgcacac agccacgact tcgacctcga
     1021 gttcgtcgag ggcgggcacc gccacgcgat cccgggctac cgcggcctcg atgtggcggc
     1081 acccggctac caggatccgc acgagctcgc gcacgccaac gacatccgcc ggcgcttcgc
     1141 cgaccgcaac gtgacgagcg ggcagatcgt ggtgtttggc ctgacgggcg ggctgatccc
     1201 gtgtccggcg tcgatcacgg tgctgctgct gtgtctgcag ctgaagaggc tcgcgctcgg
     1261 ggcggtgctg gtgctgtgct tcagcatcgg cctcgcgctc accatggtga cgaccggggc
     1321 agtggctgcg ctcggggtac agcacgccgc ccgccgcttc agcggtttcg gtgagctggc
     1381 ccgcaaggcg ccgtatttct ccggcgccgt catcatgctg gtgggatttt atgtgggcta
     1441 ccaggggctg cacgcgctgg ccgtgagccg cgtgtgagcg cgcctggatt gcggatgaag
     1501 ccggcgcgag agtagggtgc acggcatgag gctctcctcg tatcgggcac tcgtcgtgtt
     1561 tcttctcggc tgcggcatcg ccgtggctgc cacggggccg aaggaggccg cgccggctac
     1621 gggtgtcgcg accccggcgg cgagcgccgc cgagccaccg ggcacggccc tcgtactgga
     1681 gatcaacggg ccgatcgggc ctgcgacctc gcgc
//
LOCUS       DATLKL010000757         1627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_133361, whole genome shotgun sequence.
ACCESSION   DATLKL010000757 DATLKL010000000
VERSION     DATLKL010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133361"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VM713_10285"
     CDS             <1..227
                     /locus_tag="VM713_10285"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HVH84693.1"
                     /translation="DLVAKETAEIGVLQGYLPAQMSDAEIDELIAQSIAASGAASVKD
                     MGKVMALVKVKAQGRADLGAVSARIKQKLS"
     gene            282..>1627
                     /gene="dnaG"
                     /locus_tag="VM713_10290"
     CDS             282..>1627
                     /gene="dnaG"
                     /locus_tag="VM713_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HVH84694.1"
                     /translation="MGRIPQHFIDELIARADIVEVIGSRVQLKKSGREYKACCPFHNE
                     KTPPFWVSPDKQFYHCFGCGKHGTVLGFLMDYDHLAFPEAVEELASRLGLEVPREGGG
                     DGGAPRAEEPLYELMARVARFYAESLQRDVRARDYLAKRGLTPETLERFQIGYAANSW
                     NEVLKRFGTDETQRQRLSDTGLIIERERGQTQLSERHYDRFRDRIMFPIRDSRGRVIA
                     FGGRIVDAGEPKYLNSPETVLFHKGRELYGLFETRRARTSLKRLVIVEGYMDTVRLHQ
                     EGIDYAVATLGTATTAEHFRRIFRLVPEVVFAFDGDRAGRAAAWRALQLSLPEAREGR
                     QIRFLFLPEGHDPDTLVAAEGRAAFEQRLGHSLPLSEYLVRELSEQSDLSHADGRARF
                     AESARPLYQKVPAGVYRELLLGRLAQVVGLAPERLEELWSQAPGAPAAPAAAPAQRP"
BASE COUNT          256 a          573 c          551 g          247 t
ORIGIN      
        1 cggatctggt cgccaaggag accgccgaga tcggcgtgct gcagggctat ctgcccgcgc
       61 agatgagtga cgcggagatc gacgagctga ttgcacagtc gatcgccgcg agcggcgccg
      121 cctcggtgaa ggacatgggc aaagtgatgg ccctcgtgaa ggtcaaagcg caggggcgcg
      181 ccgacctcgg cgcggtcagc gcgcggatca agcagaagct cagctgattc ctttatagtc
      241 tctcaaggag cgcggccccc aggaccacgc tcctgatctc cgtgggacgc atcccgcagc
      301 atttcatcga cgagctcatc gcccgcgccg acatcgtgga ggtgatcggc tcgcgcgtgc
      361 agctgaagaa gtcggggcgt gagtacaagg cctgctgccc cttccacaac gagaagacac
      421 cccccttctg ggtgagtccc gacaagcagt tctatcactg cttcggctgt ggcaagcacg
      481 gcacggtgct cggcttcctg atggactacg accacctcgc ctttcccgag gcggtcgagg
      541 agctcgcctc ccgcctcggc ctcgaagtcc cgcgcgaggg tggtggcgat ggcggcgcgc
      601 cgcgcgccga ggagccgctt tatgagctca tggcgcgggt cgcacgcttc tacgccgaga
      661 gcctgcagcg cgacgtccgg gcgcgcgact atctcgcgaa acgcggactc acccccgaga
      721 cgctcgagcg cttccagatc ggctacgccg caaattcctg gaacgaggtg ttgaagcgtt
      781 tcgggaccga cgagacgcag cgccagcgac tctccgatac ggggctcatc atcgagcgtg
      841 agcgcggcca gacgcagttg agcgagcgcc actacgaccg cttccgcgac cgcatcatgt
      901 tcccgatccg cgatagccgc ggccgggtga tcgccttcgg cgggcgcatc gtcgacgccg
      961 gcgagcccaa gtacctgaac tcccccgaga cggtgttgtt ccacaagggg cgcgagctct
     1021 atggtctctt cgagacgcgc cgtgcgcgca ccagcctcaa acgcctcgtg atcgtcgaag
     1081 gctacatgga cacggtgcgt ctgcatcagg aaggcatcga ctacgccgtg gcgacgctcg
     1141 gcaccgcgac caccgccgag cacttccgtc gcatcttccg tctggtcccg gaggtggtct
     1201 tcgccttcga cggcgatcgc gccgggcgag ccgcggcgtg gcgggcgctg cagctctcgc
     1261 tccccgaggc gcgcgagggg cgccagatcc gcttcctgtt cctgcccgaa ggccacgacc
     1321 ccgacacgct ggtggccgcc gagggacgcg cggccttcga gcagcgcctc ggccactcgc
     1381 tccccctctc cgagtacctg gtgcgcgagc tctccgagca gagtgacctg agccacgccg
     1441 acgggcgggc gcgcttcgcc gagagcgcgc gcccgctcta ccagaaggtc cctgcggggg
     1501 tgtaccgcga gctgctgctc gggcgcctcg cgcaggtcgt ggggctggca ccggagcggc
     1561 tcgaggagct gtggagccag gcgcccggcg cccccgcggc gccggccgcg gcgcctgcgc
     1621 agcgccc
//
LOCUS       DATLKL010000758         1515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_8964, whole genome shotgun sequence.
ACCESSION   DATLKL010000758 DATLKL010000000
VERSION     DATLKL010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8964"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VM713_10295"
     CDS             <1..417
                     /locus_tag="VM713_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84695.1"
                     /translation="SLIERADTSAGWRYRKAREFPWDARNVESAKSLRRLETALSDLR
                     PDHDLWSRYAAICSRAAGDECLRLVEIEYAMLHSYGFSIHGAAGSAEGFLLELLPALE
                     LELAAPAHDGEEAEPGTGIVDPLQEGQTSQTQRLAG"
     gene            complement(525..600)
                     /locus_tag="VM713_10300"
     tRNA            complement(525..600)
                     /locus_tag="VM713_10300"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(565..567),aa:Thr,seq:cgt)
     gene            complement(637..1068)
                     /locus_tag="VM713_10305"
     CDS             complement(637..1068)
                     /locus_tag="VM713_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HVH84696.1"
                     /translation="MTSLDTAPSGGPTRCEKRLSACGIRPTAQRVRIASVLLSAPQHL
                     SAEQILANLRDSGARVSKATVYNTLNLFASRGLIRQLSVDGSRAWFDSNVDPHYHFHD
                     LASGALIDVPVPEVEFSRLPPPPPGTEVAGIDLVIRLRKKQ"
     gene            complement(1120..>1515)
                     /locus_tag="VM713_10310"
     CDS             complement(1120..>1515)
                     /locus_tag="VM713_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HVH84697.1"
                     /translation="EVIPVKRIPKTTSGKIQRHLLEEGYVDGEFDAELAELAALRAAQ
                     RGPAHRERSELEEQLKTICDAALEGKRVDVNDNLFEVGASSLKLIEIHEQIDRLYPGQ
                     IDLTELFDFPTIAELARHLEGKLAGAPTS"
BASE COUNT          266 a          466 c          522 g          261 t
ORIGIN      
        1 agcctgatcg agagggctga tacctctgcg ggatggagat atcgcaaggc gcgcgaattc
       61 ccctgggacg cacgcaatgt cgagagcgcc aagtcgctgc ggcggctgga aacggctctg
      121 tccgatctgc gccccgacca cgacttatgg agcaggtacg ccgcgatctg cagccgggcg
      181 gcgggagatg agtgcctgcg gttggtcgag atcgaatacg caatgctgca ctcgtacggg
      241 ttctcgattc acggcgccgc gggaagcgcg gaagggtttc tgctcgagct tctcccggcc
      301 ctcgagctcg agctggcagc gcctgcgcac gacggcgagg aggcagagcc gggaacgggc
      361 atcgttgacc ccctccagga gggccagacc agccaaaccc agcgtcttgc ggggtagcgc
      421 cagggcgcta ccccgtactc ccagccgccg ggccgctgct tagatccgtc ccggaaagtg
      481 gctgtgcagc tgcgtcagga atctctcggc cagtttctag cgggtggtgc cgggaatagg
      541 attcgaacct acgacccgcg cattacgaat gcgctgctct accaactgag ctatcccggc
      601 ccgcgggacc gacaattcta cggaaaaata agaaatctac tgctttttac gcagccggat
      661 gaccaggtcg atgcccgcga cctcggtgcc gggtggtggc gggggcaggc ggctgaactc
      721 gacctccggg acgggaacgt cgatcagcgc gccgctcgcg aggtcgtgaa agtgatagtg
      781 gggatcgaca ttggagtcga accaggcgcg cgacccgtcg accgagagct gacggatgag
      841 accgcgcgag gcaaacaggt tcagggtgtt gtacacggtg gccttggaca cgcgggcgcc
      901 gctgtcgcgc aggttcgcga gtatctgctc ggccgagagg tgctgcggcg ccgagagcag
      961 cacggacgcg atgcgcacac gctgcgctgt cgggcggatc ccgcaggcgc tgagccgctt
     1021 ctcgcagcga gtcgggccgc cggagggggc cgtgtcgaga ctggtcatgc gcgcattata
     1081 ggcactcagt gcctcaagtc aaaaataatg ccttagattt catgacgttg gagccccagc
     1141 gagcttgccc tcgagatgcc tcgcgagctc ggcgatcgtc ggaaagtcaa agagctcggt
     1201 caggtcgatc tggccgggat agagccggtc gatctgctcg tggatctcga tgagcttcaa
     1261 ggagctcgcg cccacctcga agagattgtc gttcacgtcg acgcgcttgc cctcgagggc
     1321 cgcgtcgcag atggtcttca gctgctcctc gagctcgctg cgctcgcggt gcgcgggccc
     1381 gcgctgcgcc gcgcgcagcg cggcgagctc ggcgagctcg gcgtcgaact cgccgtcgac
     1441 atagccttcc tcgagcaggt ggcgctggat cttgccgctc gtcgtcttcg ggatgcgctt
     1501 gaccggaatg acctc
//
LOCUS       DATLKL010000759         2521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_31767, whole genome shotgun sequence.
ACCESSION   DATLKL010000759 DATLKL010000000
VERSION     DATLKL010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31767"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="VM713_10315"
     CDS             complement(<1..1918)
                     /locus_tag="VM713_10315"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVH84698.1"
                     /translation="MSVPASPRALRIFTGILRLRGWIAGAFLALTVAGAYGALHIPDD
                     LALEHLMVPGDPVARATRDFERFFPEGEEALLVLEAGDPLSPVALAAADQLEGELARI
                     PGVAPHSVLTLYRLAVPGAQVNAAQAERLRAFATGTALFRRAGLLGDHYLGIALELRV
                     HSPAERNTALAAIEARLRPLEARGAPFTAVRRVGSPWLDAWLERETGLATERSMPLFG
                     VFLLALVLIVYRSWLTLAAIVLTLAAVVAIALGLAQPLGFSHTVVSALVPLTVLVTTT
                     ATLVYLHSRYLDASEAATPLEHHARTLADKFLPCTASVFATAVGFAALAVSDIRPVRE
                     MGLWTASGLLVAWIASFSLFPALQSLLRLPVRAARAPAAAWFGTFADWLVSWTRRWRW
                     ALVGGALAMMLCGAAALFGIPGRIAPLPLETDALSYVDARERIAQDTRRFRQLSGLDV
                     VEMWLQTPPGHALDPDFLRALEELSERLERDPRITAVDGPTSVLRWAHYVQTGSDQLP
                     AAPAAWPKLAADLEQILLTEPGARGYVDVADLASVKLSVRGRANLFGPVGAMRVFIER
                     ITGAWQAGQPALQGVHAQAVGRAVLAAQISERLVPTLTESFLLTASVIFTAFLFVFRS
                     PAGRLMAMIPSLFAI"
     gene            1983..>2521
                     /locus_tag="VM713_10320"
     CDS             1983..>2521
                     /locus_tag="VM713_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84699.1"
                     /translation="MSTLWAAAAVTLVFCAPGYPGQTGDAQPYLDQFAQAVAADAGWP
                     KGSLAAVYDPTESGGLARLEKPDAGLAFVPFPFLVAHGERLHLAPLVEADVVDSGARE
                     RWTLIAKRGRVQGPASLSGFTLLSTAGYAPAFIRGAALGKWALPSDVRIEATGQVLSA
                     LRRAAAGEPVAVLLDQTGST"
BASE COUNT          421 a          868 c          922 g          310 t
ORIGIN      
        1 gaatcgcaaa cagcgagggg atcatcgcca tcagccgccc cgcagggctg cggaacacga
       61 acaggaaggc ggtgaagatg acactcgccg tgagcaggaa gctctccgtg agcgtcggca
      121 cgaggcgctc gctgatctgc gccgcgagta ctgcgcggcc aacggcctgg gcgtgcacgc
      181 cctgcagcgc gggctggccc gcctgccacg cgcccgtgat ccgctcgatg aagacgcgca
      241 tcgcgcccac gggcccgaag agattcgcgc gtccgcggac gctgagcttc acgctcgcaa
      301 gatccgccac gtcgacgtaa ccgcgcgctc ccggctcggt gagcaggatc tgctcgaggt
      361 cggcggcgag cttcggccag gcggccggcg cggccggcag ctgatcggac ccggtttgaa
      421 cgtagtgagc ccagcgcagc accgaggtcg ggccatcgac ggcggtgata cgcggatcgc
      481 gctcgaggcg ctcgctgagc tcctcgaggg cgcgcaggaa gtccgggtcg agggcatggc
      541 cgggaggcgt ctgcagccac atctccacga cgtcgagacc gctcagctgg cggaagcgcc
      601 gtgtgtcctg cgcgatgcgc tcccgcgcat cgacgtaact cagcgcgtcg gtctcgagcg
      661 gcagaggcgc gatccggccc ggaatcccga agagtgccgc cgcgccgcac aacatcatgg
      721 cgagcgcgcc gccgacgagc gcccagcgcc agcgccgggt ccaggacacg agccaatcgg
      781 cgaaggtccc gaaccaggca gcggccggcg cgcgcgccgc gcgcaccggc agccgcagca
      841 gtgactgcag cgccggaaag aggctgaagc tggcgatcca cgctaccagc agaccgctcg
      901 ccgtccacag tcccatctcg cgcaccgggc ggatgtcgga caccgcgagc gcggcaaagc
      961 cgacagcggt cgcgaaaacc gaggcggtgc aagggaggaa cttgtcagcg agcgtgcggg
     1021 cgtgatgctc gaggggcgtc gccgcctcgc tcgcgtcgag gtagcgcgag tgaaggtaga
     1081 cgagcgtggc cgtcgtggtc accaggacgg tgagcggcac caacgccgag accaccgtgt
     1141 gcgagaaacc gaggggctgg gcgagaccca gtgcgatcgc caccacggcg gcgagtgtca
     1201 gcacgatggc cgcgagcgtg agccaggagc gatagacgat cagtaccagc gcgagcagga
     1261 agacgccgaa cagcggcatg gagcgctccg tcgcaagacc cgtctcgcgc tcgagccagg
     1321 catcgagcca cggggagccg acgcgccgca ccgccgtgaa gggcgcgccg cgggcttcga
     1381 gcggccgcag ccgcgcctcg atggccgcga gcgccgtgtt gcgctcggcc ggggagtgca
     1441 cgcgcaactc gagcgcgatg ccgagatagt gatctcccag aagcccggcg cggcgaaaga
     1501 gcgcggttcc ggtcgcgaag gcgcgcagcc gctcggcctg cgccgcattc acctgggcgc
     1561 cggggactgc caggcggtag agcgtgagga cgctgtgcgg cgctactcca gggatgcgcg
     1621 ccagctcacc ctcgagttgg tcggcagccg cgagcgccac cggactcagc ggatctcccg
     1681 cctcgaggac gagcagtgcc tcctccccct cgggaaagaa acgctcgaag tcccgcgtcg
     1741 cgcgcgcgac cgggtcgccc ggcaccatga ggtgctcgag ggcaagatcg tccgggatgt
     1801 gcagcgcccc gtacgccccg gcgacggtga gagccaggaa ggcgccggcg atccagcccc
     1861 tgagtcgcag gatgccggtg aagattcgta gagccctggg cgaggcgggc acactcatgg
     1921 aaaatgggcg ctcctgccgg gccgcgtggg ttcacggcgc gcgctgatga tagcctaaac
     1981 gcatgagcac cctgtgggcg gccgccgcgg tcacactcgt cttctgcgct cccggatatc
     2041 ccggtcagac cggcgatgcg cagccctatc tcgatcagtt cgcgcaggcg gtcgccgcgg
     2101 acgccggctg gccgaagggg agcctcgcgg cagtctacga tccgacagag tccgggggcc
     2161 tggcgcgtct cgagaaaccc gatgcgggac tcgccttcgt gccctttccc ttcctggtcg
     2221 cgcacgggga gaggctgcat ctcgcgccgc tcgtcgaggc cgatgtcgtc gactcaggcg
     2281 cccgcgaacg gtggacgctc atcgcgaagc gcggtcgcgt gcaaggtccc gcatcgctct
     2341 cgggattcac gctcctgagc acggcgggct acgccccggc attcattcgc ggcgcggcgc
     2401 tcgggaagtg ggcgctcccg agcgacgtcc gcatcgaagc gaccggccag gtgctgagcg
     2461 cgctgcggcg cgcggcggcg ggtgagcccg tcgcggtact gctcgatcag accgggagca
     2521 c
//
LOCUS       DATLKL010000760         3371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_54228, whole genome shotgun sequence.
ACCESSION   DATLKL010000760 DATLKL010000000
VERSION     DATLKL010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54228"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VM713_10325"
     CDS             complement(<1..200)
                     /locus_tag="VM713_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH84700.1"
                     /translation="MPLILIVDDSPTEVHVMQKALEKHGYRTAAAADGAEGVRLAREM
                     SPDLIFMDIVMPGMNGYQATRAL"
     gene            complement(206..568)
                     /gene="pilG"
                     /locus_tag="VM713_10330"
     CDS             complement(206..568)
                     /gene="pilG"
                     /locus_tag="VM713_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014720321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HVH84701.1"
                     /translation="MKVLVIDDSKTIRRTAETLLAKEGCEVFTAIDGFDALAKIADHQ
                     PDIVFVDIMMPRLDGYQTCSLIKHNKVFRAIPVIMLSSKDGLFDRARGRIVGSEHYLT
                     KPFTKDELLSAIETHIGR"
     gene            909..1886
                     /gene="gshB"
                     /locus_tag="VM713_10335"
     CDS             909..1886
                     /gene="gshB"
                     /locus_tag="VM713_10335"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HVH84702.1"
                     /translation="MSTARRLGVVMDPIGAIAYPKDSTLAMLLAAERRGFALSYLELG
                     DLMLRDGIAIGRTRPLSVRADPKGWFTLGEAATEPLARLDCLLMRKDPPFDTEYIYST
                     YILERAEAQGVLVVNRPQGLRDMNEKVYTAWFPQCCAATLLTRDMGEMGAFLREHGRI
                     VVKPLAGMGGRSIFVLGSGDPNARVIFETLTHYGQRFAIVQRYLPEITTSGDSRVLLV
                     DGAPVPYALARIPTGEDHRGNLAAGARGVGRPLNERDRWLAREIGPAMAERGMMFVGL
                     DVIGGYVTEINVTSPTGIRELDKQFGTDIGAMLIEAVERRLDTAPRRSG"
     gene            1895..2806
                     /locus_tag="VM713_10340"
     CDS             1895..2806
                     /locus_tag="VM713_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HVH84703.1"
                     /translation="MGKSLARRALALREGRPPAWDRLLTMVFFVALLHGLVILGITFN
                     AQAGADEGAPGLKVLLVSDELPEARENPTASYLAQRTQLGSGSTREPVAPRAPPAALR
                     VLKHPGSPQGDSLATRGDMKGADSERVLTTTAWVTDVRYLADLGATGSQRNRPLLFDE
                     PPVAPPGPTDEPGPAQLTGPKRDELWVTADTRAASLAPYLDAWRRKDERIGTLNFPAA
                     ARSALGDTSPVLEVGINANGTLDRAVIRRTSGNPELDQAALAILKLASPFDPFPPELA
                     AEHSVLRFAYEWQFVGGRAQRGSVSVP"
     gene            2803..>3371
                     /locus_tag="VM713_10345"
     CDS             2803..>3371
                     /locus_tag="VM713_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HVH84704.1"
                     /translation="MRVAAAVPILGGMAGKQASSGGATPPESGSGGSEPGSGTPVFAD
                     GGLGSLTNHLLIAMPQLNDPNFAQTVALICEHTDRGALGIVLNKPLPMELSDVLSQMK
                     LEPTSETIGEQPVLRGGPVHTDRGFVLHRPGGQWDHTHRVSDTIQVTTSRDVLAAMAR
                     GEGPSDAFIALGYAGWESGQLERELKENA"
BASE COUNT          545 a         1185 c         1124 g          517 t
ORIGIN      
        1 agcgcgcgcg tcgcctggta gccgttcatg cccggcatga cgatatccat gaagatcagg
       61 tcgggactca tctcgcgcgc cagccgcaca ccctcggcgc cgtcggcagc ggcggccgtg
      121 cgatagccgt gcttctcgag cgccttttgc atgacgtgca cttcggtcgg tgagtcgtca
      181 acgatcagga tcaggggcat tgccatcatc tcccgatatg agtctcgatc gcggagagca
      241 gctcgtcctt ggtgaagggc ttggtgagat agtgctcgga gccgacgatc cggccgcgcg
      301 cacgatcgaa gaggccgtcc ttggaggaca acatgatcac cggaatggcg cggaagacct
      361 tgttgtgctt gatgagcgag caggtctgat agccgtccag gcgcggcatc atgatgtcga
      421 cgaagacgat gtcgggctgg tggtcggcga tcttggcgag cgcatcgaag ccgtcgatcg
      481 ccgtgaacac ctcgcagcct tccttggcga gcagcgtctc ggcggtgcgg cggatcgtct
      541 tgctgtcgtc gatcaccagt actttcagtc cctggagctt agcgctgctg ccggtcacgg
      601 tcaggcaact ccttccttaa aggccacaag gcgtggcggc cagagaaatc gccgcaaagt
      661 aaggccacga aatcgggctg gagcacccag cggcgaccgt tcaaacacag gcagccgtag
      721 tttcacggtc cccttcacgg cgaggcaagc acagccgggt tttaacatat cggtataccc
      781 cctgctatgc cacgcacgtc acgtttttcg aaggtcgggc gggggcttac ggctgaggcc
      841 gcggcagccc cggtagcgcg cgcgcggggc agacagatcc ccgcggggct gagcataatc
      901 gtcccccgat gagcacggcc agacgactcg gcgtggtgat ggatccgatc ggcgccatcg
      961 cctatccgaa ggactcgacg ctcgcgatgc tgcttgccgc cgagaggcgc ggtttcgccc
     1021 tcagctacct cgagctcggc gacctcatgc tgcgcgacgg catcgccatc ggccgcaccc
     1081 ggcccctgag cgtgcgggcg gatcccaagg ggtggttcac cctcggggag gcggcgaccg
     1141 agcccctcgc ccggctcgac tgcctcctca tgcgcaagga cccgcccttc gacaccgagt
     1201 acatctacag cacctacatc ctcgagcgcg ccgaggcgca gggggtgctg gtggtcaacc
     1261 ggccgcaggg gctgcgcgac atgaacgaga aggtctacac cgcctggttc ccgcagtgct
     1321 gcgccgcgac gctgctgacg cgggacatgg gggagatggg ggcgtttctg cgcgagcacg
     1381 gccgcatcgt cgtcaagccg ctcgcgggca tgggcgggcg ctcgatcttc gtccttgggt
     1441 ctggcgaccc gaacgcgcgc gttatcttcg agacgctcac ccactatgga cagcgcttcg
     1501 cgatcgtgca gcgctatctc cccgagatca ccacctcggg cgactcgcga gtgctgctgg
     1561 tggacggcgc gccggtgcct tacgcgctcg cgcgcatacc cacgggcgaa gatcaccgcg
     1621 gcaacctcgc cgccggtgcc cggggcgtcg gccggccgct gaatgagcgc gatcgctggc
     1681 tcgcgcgcga gatcgggccg gcgatggccg agcggggcat gatgttcgtc ggcctcgacg
     1741 tgatcggcgg ctacgtcacc gagatcaacg tcacgagccc caccggcatt cgcgagctcg
     1801 acaagcagtt cggcaccgac atcggtgcaa tgctcatcga agccgtcgag cggcgcctcg
     1861 ataccgcacc gcgccggagc ggctgagagg cgccatgggg aaatcgctgg cacggcgcgc
     1921 gctcgctttg cgcgaaggcc gcccccccgc gtgggaccgc ctgctcacca tggtcttctt
     1981 cgtcgcgctt ctgcacggac tcgtgatcct cggcatcact ttcaacgccc aggcaggagc
     2041 ggacgagggc gcgccgggac tgaaagtcct cctggtctcg gacgagctgc ccgaagcgcg
     2101 ggagaatccc accgccagct acctcgctca gcggacccag ctcggctccg gcagtacgcg
     2161 cgagccggtg gcgccgcgcg cgcccccggc ggcgctgcgg gtactcaagc acccgggatc
     2221 cccgcagggc gattcgctcg cgacccgcgg cgacatgaag ggcgccgact ccgagcgcgt
     2281 cctgaccacc accgcctggg tgacggacgt acgctatctc gccgacttgg gtgccaccgg
     2341 ctcgcagcgc aatcggccgt tgctcttcga cgagccgccg gtcgcgccac cgggtccgac
     2401 cgacgagccg gggcccgcgc agctcacggg accgaagcgc gacgagctct gggtcacggc
     2461 cgacacccgc gccgcctcgc tcgcgcccta tctcgatgcc tggcggcgca aggacgagcg
     2521 catcggcacg ctcaacttcc cggccgccgc ccgctctgcc ctcggggaca ccagccccgt
     2581 gctcgaagtc ggcatcaacg ccaacggcac gctcgaccgc gcagtcatcc gccgcacgag
     2641 cggcaacccc gagctcgacc aggcggcgct ggcgatcctc aagctcgcga gccccttcga
     2701 cccctttcct cccgagctcg ccgccgagca cagcgtgttg cgttttgcct acgaatggca
     2761 gtttgtaggc gggcgcgcgc agcgcggctc ggtttcggtt ccttgagagt cgcggccgcc
     2821 gtgcccatac ttgggggcat ggccggcaag caagcatcga gcggcggggc cactcctccg
     2881 gagagcggct caggcggcag cgagcctggc agcgggaccc cggtgttcgc cgacggcggc
     2941 ctcgggagcc tcaccaatca cctgctgatc gccatgccgc agctcaacga tccgaacttc
     3001 gcgcagacgg tggcgctcat ctgcgagcac accgacaggg gcgcgctcgg catcgtgctc
     3061 aacaagccgc tgccgatgga gctctcggac gtgctctcgc agatgaagct cgagccgacc
     3121 agcgagacga tcggcgagca gccggtgctg cgcggcggcc cggtgcacac cgaccgcggc
     3181 ttcgtgctgc atcggcccgg cgggcagtgg gaccacacgc accgggtctc cgacaccatc
     3241 caggtgacaa cctcgcgcga cgtgctcgcc gccatggcgc gcggcgaagg cccctcggac
     3301 gccttcatcg cgctcggcta cgccggctgg gaatccggcc agctcgaacg cgagctgaag
     3361 gaaaacgcct g
//
LOCUS       DATLKL010000761         2790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_43031, whole genome shotgun sequence.
ACCESSION   DATLKL010000761 DATLKL010000000
VERSION     DATLKL010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43031"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VM713_10350"
     CDS             complement(<1..243)
                     /locus_tag="VM713_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84705.1"
                     /translation="MAAILSAPTIALAAPGDLEYPADQAAGAAEAGGLAEVVVTAERR
                     SETVQNVPITIQAITGDQLSQMQVQTFDDVLKYLPNV"
     gene            complement(529..2235)
                     /locus_tag="VM713_10355"
     CDS             complement(529..2235)
                     /locus_tag="VM713_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HVH84706.1"
                     /translation="MTKLHTGALAEVEQRCRALLEARPHDGMVWKVLSVALLRQGKDA
                     LPALQRAVELLPDDAETHLNLGIALATAGARAEAVASYRQALALNPASMEGLSHLGDV
                     LRELGEGEEALAVRRRAIELEPQRAEHHNNLGSILFDLRRLDEAAAAFRAALALHPDN
                     ARALLGLAGTTRMRGRAAEAEEICRAALALEPNNVEGLSLLGELYADRGQFEEGRALL
                     ERALAINPALPSVYSSLVGSRRMTDADGLWHQQVAALLSRPLPHGHEVDLRYALGKYF
                     DDLGRYDEAFDSYRRANELARRHKAQYNRGRLEQRVERIIDTFDAEFLGARCAAGSPS
                     ELPVLIIGMPRSGTSLAEQILASHPAVFGGGELRFWEGAFGIFEAERAAGVSAAEILA
                     QFAQDYLERVQALSGTAARVIDKMPANFLYAGLVHAALPRARIIHMQRHPLDTCLSIY
                     SQNFFRMGSYSNDLEDLAHYYSQYLRIMRHWRTVLPAAAMLEIPYEALIADQESWTRR
                     MLEFVGLPWDPRCLDFQNTERIVITASRWQVRQKINSASAGRWRNYAKHLRPLAHLVN
                     LT"
     gene            complement(2367..>2790)
                     /locus_tag="VM713_10360"
     CDS             complement(2367..>2790)
                     /locus_tag="VM713_10360"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HVH84707.1"
                     /translation="IIDARREPMACCFSNLKQLFANGQEFTHGIEEIARYYRTYLELM
                     EHWDRVLPGRILRVYHEDVVENLASNVQRMLEFCGLPFEPQCLDFHETQRSVRTASSE
                     QVRQAIFREGLDQWKHFEPWLGPLKDALGDALTRYREP"
BASE COUNT          453 a          942 c          907 g          488 t
ORIGIN      
        1 cacgttcggc aggtacttga ggacgtcgtc gaacgtctga acctgcatct gcgaaagctg
       61 atcgcccgtg attgcctgga ttgttatcgg gacgttctgc accgtttcgg agcggcgctc
      121 ggcggtcacg acaacttcgg caagtccgcc ggcctcggcc gcgcccgcgg cctgatccgc
      181 cgggtactcc aggtcgcccg gagcggccag cgcaatcgtc ggcgcgctca gaatcgcggc
      241 cacggccgcg gaaatcttcg gattagcctt cattgcgttg ctccccgggg gatttacttg
      301 gttaattccc ccacgcgact gcagccaccc tcgcgggcgg ttcttgtggt gacgtttata
      361 ccattcggtc agcaggcgct agaacaaacg cgccatcgga gagtcgtctg cgctctccgg
      421 ccttcgtcgt ctgccaagct tttcacacga ttgtgacagt cagaatactt gcagtcgcgg
      481 ccagcgaccg aactgagaag cgttaccgct tagcgagcgc cgcgagcatc acgtcaggtt
      541 caccaggtgc gccagcggtc gcaggtgctt cgcatagttg cgccagcgcc cggccgacgc
      601 gctgttgatc ttctgacgca cctgccagcg gctcgccgtg atgacgatgc gctcggtatt
      661 ctgaaagtcg aggcagcgcg gatcccacgg cagaccgacg aactcgagca tccgccgtgt
      721 ccagctctcc tgatcggcga tcagcgcctc gtacgggatc tcgagcatcg ccgccgcggg
      781 cagcaccgta cgccagtgcc tcatgatgcg caggtactgg ctgtaataat gcgccagatc
      841 ctcgagatcg ttggagtacg agcccatccg gaagaaattc tgggagtaga tcgacaggca
      901 ggtgtcgagc ggatggcgct gcatgtggat gatgcgggcg cgcggcagcg ccgcgtggac
      961 caatccggcg tagagaaaat tggcaggcat cttgtcgatt acgcgcgcgg cggtgcccga
     1021 cagcgcctgc acccgctcga ggtagtcctg cgcgaactgc gccaggattt cggcggcgga
     1081 cacgcccgcc gccctttccg cctcgaagat gccgaaggca ccctcccaga accgcaattc
     1141 tcccccgcca aagaccgccg gatgtgaggc gaggatctgc tcggcgagcg aggtgcccga
     1201 tcgcggcatg ccgatgatca agacgggcag ctcggagggc gagcccgcgg cgcagcgcgc
     1261 gcccaggaat tccgcgtcaa aggtgtcgat gatccgctcg acacgttgct cgagcctgcc
     1321 gcggttgtac tgcgccttgt gacgccgcgc gagctcgttg gcacgccggt agctgtcgaa
     1381 cgcctcgtcg tagcggccga ggtcgtcgaa gtacttgccg agcgcatagc gcaaatcaac
     1441 ctcgtgtccg tgcggcagcg gcctcgacag cagcgctgcc acctgctggt gccagaggcc
     1501 gtcggcgtcg gtcattctcc ggctgccgac caggctgctg tacacggacg gcaaagccgg
     1561 attgatcgcc agcgcacgct cgagcagcgc ccgcccttcc tcgaactgtc cgcggtcggc
     1621 gtacagctcg ccgaggagcg acagtccttc gacgttgttc ggctcgagcg cgagtgcagc
     1681 gcgacagatc tcctccgcct cggcagcccg tccgcgcatg cgcgttgtcc cggccaggcc
     1741 gagcagagcc cgcgcgttgt ccgggtgcag cgccagggcg gcgcgaaagg cggcagccgc
     1801 ttcatccagc cggcgcagat cgaacagaat gctgccgagg ttgttgtggt gctcggcgcg
     1861 ttgcggctcg agctcgatcg ctctgcgacg caccgcgagc gcctcctcgc cctcgccgag
     1921 ctcacgcagc acgtcgccga gatggctcag tccctccatg ctggcgggat tcaacgccag
     1981 cgcctgccgg tagctcgcca cggcctcggc gcgcgcgccg gccgtggcca gcgcaatgcc
     2041 gagattcaga tgcgtctcgg catcgtccgg caggagctct accgcccgct gcagtgccgg
     2101 cagcgcatcc ttcccctgcc gcagcaaagc cacgctcagg accttccaca ccatgccgtc
     2161 atgcggacgc gcttcgagga gcgcccggca gcgctgctcg acttcggcga gtgctccggt
     2221 gtgcagcttc gtcacgagct cggtcagctc gcgttggtgc gcggggccgc gaggcagctt
     2281 agcgtccgcc accccgtggc agtgcttgta tttccggccg ctgccgcagg ggcagggctc
     2341 gttgcggccc ggcttggttg cgatactcac ggctcgcggt agcgcgtcag cgcatcgccg
     2401 agcgcatcct tgagcggccc gagccacggc tcgaagtgct tccactgatc gagcccctcg
     2461 cgaaagatcg cctggcggac ctgctccgag ctcgccgtgc gcacgctgcg ctgggtctcg
     2521 tgaaagtcca ggcattgggg ctcgaacggc aacccgcaga actccagcat gcgctgcacg
     2581 ttgcttgcaa gattctcgac cacgtcctcg tggtagacgc gcaggatccg cccggggagg
     2641 acgcggtccc agtgctccat cagctcgaga taagtgcggt aatagcgcgc gatctcctcg
     2701 atgccgtggg tgaactcctg accgttagcg aacagctgct tgagattgct gaagcagcag
     2761 gccatcggct cgcggcgcgc atcgatgatg
//
LOCUS       DATLKL010000762         1674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_20337, whole genome shotgun sequence.
ACCESSION   DATLKL010000762 DATLKL010000000
VERSION     DATLKL010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20337"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..797)
                     /locus_tag="VM713_10365"
     CDS             complement(72..797)
                     /locus_tag="VM713_10365"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HVH84708.1"
                     /translation="MALLALASEAATGALPDFSGQPVSVDAASSEVDYKTNTVVFKNV
                     VISQGAMRVQAEHARATGLNFANSKWNFDGNVRIDAEQRGNLRSDQAVVEFKDNHIAR
                     ATITGKPAEFEQQRANSEAVARGHADEILYDVNDGTVRLANDAWVCDGQNQMWGPLLV
                     YNIREQQIQAVTAPAASGGSGDQRVHISIVTHPGKPESAGCEAPKPETPKADATKADA
                     PKADTAKPAAEQPAAAAESAPKS"
     gene            complement(820..1380)
                     /gene="lptC"
                     /locus_tag="VM713_10370"
     CDS             complement(820..1380)
                     /gene="lptC"
                     /locus_tag="VM713_10370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HVH84709.1"
                     /translation="MMYRLVAVLAAVALVVGLVLLSGPQREAVVTSTAAAPARDPGYA
                     ARDARLVQTGADGQPLYSLDAARMTQRPDDNAVELTQVQMGFRATGGEQWTARADHGE
                     LGQDSGVVLLDGNVHIAGLLPGTQDPAEIAAEHLSLDTRAQIVTTPDPVTLTVSGHEL
                     HAQGLLARLKEGRVQLESAVHGSYLP"
     gene            complement(1395..>1674)
                     /locus_tag="VM713_10375"
     CDS             complement(1395..>1674)
                     /locus_tag="VM713_10375"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HVH84710.1"
                     /translation="LKVRHVQQSVANKRAEFEQLSARLKLARSVCACVADDLPDVPLM
                     NAVALPFAVADAHPAVRRAAAVVTRQSGGRGAVREVCDLLLAGHGRGR"
BASE COUNT          228 a          587 c          585 g          274 t
ORIGIN      
        1 gtacagcgac aggtcgcgca cgaccggacg gttcttgtag ctcttggcga ggcccgtggc
       61 cctcaggacc gtcatgactt cggggccgac tcggccgccg ccgcaggctg ttccgccgca
      121 ggcttcgccg tgtcggcctt cggagcatcg gccttcgtag catcggcctt cggggtctcg
      181 ggcttcggcg cctcgcagcc cgcggactcg ggcttgccgg ggtgcgtcac gatgctgatg
      241 tgcacacgct ggtccccgct gccgccgctg gccgcgggcg ccgtgacggc ctggatctgc
      301 tgctcgcgga tgttgtagac gagcagcgga ccccacatct ggttctggcc atcgcacacc
      361 caggcgtcgt tcgcgaggcg cacggtgccg tcgttcacgt cgtagagaat ctcgtcggcg
      421 tgaccgcggg cgacagcctc ggagttcgcc cgctgctgct cgaactcggc cggcttgcca
      481 gtgatcgtgg cacgcgcgat gtgattgtcc ttgaactcga ccacggcctg atcggagcgc
      541 agattgccgc gctgctcggc gtcgatgcgc acgttgccgt cgaagttcca cttgctgttg
      601 gcaaagttca gccccgtggc gcgcgcgtgc tcggcctgca cgcgcatggc gccctgggag
      661 atgacgacgt tcttgaacac caccgtattg gtcttgtaat ccacctcgga ggacgcggcg
      721 tccaccgaca cgggctgccc gctgaagtcc ggcagggctc ccgtcgcagc ctcgctcgcc
      781 agggccagca aggccacggc caggagcaag gcggcggatt tacggcagat acgagccatg
      841 caccgccgat tctagctgca cgcgaccctc cttcaagcgc gcgaggagcc cctgcgcgtg
      901 cagctcgtgg cccgagacgg tgagcgtcac cggatcgggc gtggtgacaa tctgcgcgcg
      961 cgtgtcgagg gagaggtgct cggcggcgat ctcggccggg tcctgtgtgc cggggaggag
     1021 tcccgcgatg tgcacgttgc cgtcgagcag cacgacgccg ctgtcctgac cgagctcgcc
     1081 atggtcggcg cgcgccgtcc actgctcccc gcccgtggcg cgaaaaccca tctgcacctg
     1141 ggtcagttcc acggcgttgt catcgggccg ttgcgtcatg cgcgcggcgt cgaggctgta
     1201 gagcggctgg ccatcggcgc cggtctgcac gaggcgcgcg tcgcgcgccg cgtagcccgg
     1261 atcacgcgcc ggcgctgccg cggtactcgt cacgactgcc tcgcgctgcg gtccggagag
     1321 caggaccagg cccacgacga gcgccaccgc cgcgagcact gccaccaggc gatacatcat
     1381 gacgcgagca accctcagcg cccgcggccg tggcctgcca gcaggagatc gcacacctcg
     1441 cgcaccgccc cgcggccgcc gctctggcgg gtcactaccg cggcggcgcg ccgcaccgcc
     1501 gggtgcgcgt cggcgaccgc gaaggggagg gccaccgcat tcatgagcgg cacgtcgggc
     1561 aggtcatcgg ctacgcaggc gcacacgctg cgcgcgagct tcaggcgggc gctgagttgt
     1621 tcgaattcgg cgcgtttgtt tgcgacgctc tgttgcacgt gacggacctt gagc
//
LOCUS       DATLKL010000763         2362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_178384, whole genome shotgun sequence.
ACCESSION   DATLKL010000763 DATLKL010000000
VERSION     DATLKL010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178384"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VM713_10380"
     CDS             complement(<1..340)
                     /locus_tag="VM713_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84711.1"
                     /translation="MLGAALIFVLLLVAWEAHWRAFGATPGVANNSGLWAIQRRRIDA
                     GEGDATVIVGSSRPFFDIQLPVWERLAGRRPIQLAFEGTSPLKFAEDLADDPRFTGRL
                     IIGIDPQVFFQ"
     gene            complement(480..1895)
                     /locus_tag="VM713_10385"
     CDS             complement(480..1895)
                     /locus_tag="VM713_10385"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HVH84712.1"
                     /translation="MVFNSLTFIAFFACVLLMHSLPFGWTTKKINLLIASYLFYSAWN
                     PPFVILLWVSTVVDWYAAQGLVRARRQVARHAWMLLSVVANLGLLGFFKYGQFLLDNF
                     TAVMSALGVAYHPAHYDIVLPVGISFYTFATMSYTLDVYLRRALPARNLLDYALFVTF
                     FPHLVAGPIMRPTELVPQFETPRRATAPQLRFGLALMTIGLFNKIVLADSFLSHVAES
                     VYDGDKIPGALDAWAATLAFSGQIFCDFAGYSATAIGAALCLGFAMPDNFRFPYAAVG
                     FTDFWRRWHITLSSWLRDYLYIPLGGNRHGARRTYAALMTTMLLGGLWHGANWTFVAW
                     GGLHGLYLALERALRARFSGYRPGPLVFVALGILTYMLVNLTWVFFRAKTFGKAWVVL
                     RGMFGQSGTAKPILNTFSLLAVLVIVGGMVLTHWLMRGRTLESVIARAPAALVCVVWA
                     LLAFAIVIAQGAGSAFIYFQF"
     gene            complement(2039..>2362)
                     /locus_tag="VM713_10390"
     CDS             complement(2039..>2362)
                     /locus_tag="VM713_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVH84713.1"
                     /translation="IRIGDSVVMLADTQPGATCSGPRSLGGSTVGLMIYLENVDTVFA
                     RALKAGGKSLRPLANQFYGDRSGTLEDPFGHVWTVATHVEDVPPEELERRVAAMKSSA
                     AAAAT"
BASE COUNT          466 a          764 c          803 g          329 t
ORIGIN      
        1 cctgaaagaa cacctgagga tcgatgccga tgatcaggcg tccggtaaag cgtgggtcgt
       61 cggcgagatc ctccgcgaac ttgaggggcg aggtgccctc gaacgcgagc tgaatcgggc
      121 ggcggccggc gagtcgctcc cagaccggca gctggatgtc gaagaagggg cgcgaggatc
      181 ccacgatcac cgtcgcatcc ccctcgccgg catcgatccg gcggcgctga atggcccaga
      241 ggccgctgtt gttggcgaca cccggagtgg cgccgaaggc tcgccagtgc gcctcccatg
      301 ccacgagcag cagcacaaag atcagggcgg caccgagcat gatcttaccc cagggctgct
      361 ccggcaccgg ccgcaccggg accggttgcg caattcccgg tcggtccgcc gccgtttggc
      421 gcgcgtagcg atctggcgtg ctctgtcccg cgtacccgcg ggtcagcgcg agcgcgctgt
      481 cagaactgga agtagatgaa ggcacttccg gccccctggg caatgacgat cgcgaacgcg
      541 agcagcgccc acaccacgca cacgagcgcc gccggcgcgc gggcgatcac cgactcgagc
      601 gttcggccgc gcatcagcca gtgcgtcagc accatgccgc cgacgatcac gagcacggcg
      661 aggagactga aggtattgag aatcggcttg gcggtcccgc tctggccgaa catgccacgc
      721 agtaccaccc aggccttgcc gaaagtcttg gcgcggaaga acacccaggt gaggttgacc
      781 agcatatagg tgaggatccc gagcgcgacg aacacgaggg gcccggggcg atagccggag
      841 aagcgcgcgc gcagtgcgcg ctcgagcgcg agatacagtc catgcagccc gccccacgcg
      901 acgaaggtcc agttcgcccc atgccagagc ccgccgagga gcatggtggt catcagggcg
      961 gcgtaagtgc ggcgcgcgcc gtgtcgattg ccgccgagcg gaatatagag atagtcgcgc
     1021 agccacgagg acagcgtgat gtgccagcgt cgccagaaat ccgtgaagcc aaccgccgca
     1081 tacgggaagc ggaaattgtc cggcatcgcg aaaccgagac agagcgccgc cccgatcgcg
     1141 gtggccgagt agccggcgaa gtcgcagaag atctgtccgc tgaaggcgag cgttgccgcc
     1201 caggcgtcga gcgccccggg gatcttgtcg ccgtcgtaga cactctcggc gacatgggag
     1261 aggaagctgt cggcaagcac gatcttgttg aacagcccga tggtcatgag cgccagcccg
     1321 aagcgcagct gcggcgcggt ggcgcggcgc ggtgtctcga actgcggcac gagctcggtc
     1381 gggcgcatga tcggtcccgc cacgaggtgc gggaagaagg tgacgaacag cgcgtaatcg
     1441 agcaggtttc gcgccggcag cgcgcgccgc aggtacacat ccagcgtgta cgacatcgtc
     1501 gcgaaggtat agaaggagat gccgaccggc agcacgatgt cgtagtgcgc gggatgatag
     1561 gcgacgccga gcgcggacat caccgcggtg aagttgtcga gcaggaactg cccgtacttg
     1621 aagaagccga gcaagccgag gttcgccacc accgagagca gcatccacgc atgccgcgcc
     1681 acctggcggc gcgccctgac gagtccctgt gccgcatacc agtcgaccac cgtcgacacc
     1741 cacaagagga tcacgaacgg cggattccac gccgaataaa acaggtagct ggcgatcagc
     1801 aggttgatct tcttcgtggt ccagccgaag gggagcgagt gcatgagcag cacgcacgcg
     1861 aagaacgcga tgaacgtgag agagttgaag accataggac gcctgcccgc ttcagacgcg
     1921 ggtctctata agttcttagt tgatcccccg gccgccctgg tggcggccca aggatcatgc
     1981 cttagccggg tgcggcggcg caacggctca gcacgccgcg cccagtaacg gccccggatt
     2041 aagtcgccgc cgcggcagcc gacgacttca tggccgccac gcgccgctcg agctcctcag
     2101 gcgggacgtc ctcgacgtgc gtggcgacgg tccatacgtg gccgaaggga tcctcgagcg
     2161 tgcccgagcg gtctccgtag aactgattcg ccagcggccg cagcgacttg cctccggctt
     2221 tgagcgcgcg cgcaaacacc gtgtcgacgt tttcgagata gatcatcagt ccgaccgtcg
     2281 agccgccgag tgagcgcgga ccgctgcacg tggcgccggg ctgcgtgtcc gcgagcatca
     2341 ccacggagtc cccgatcctg at
//
LOCUS       DATLKL010000764         1574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_20360, whole genome shotgun sequence.
ACCESSION   DATLKL010000764 DATLKL010000000
VERSION     DATLKL010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20360"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="VM713_10395"
     CDS             complement(<1..1492)
                     /locus_tag="VM713_10395"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVH84714.1"
                     /translation="MLNASLTTTRLGSLVIALLSAALPLEALRADETASAAGPTPAAA
                     AAPVETERTKLQEVVVTANRREESLSKVPISVSAFTQDPMDDRGIKDFQDIARFLPGV
                     SIGNSGTNAISIRGISSSAGAGTTGIYIDDTPIQMRNVGFNPDDTLPKTFDLQRVEVL
                     RGPQGTLFGSGSEGGTVRYILTQPSVTGSSTYLRSELAYTYYGQPSYEFGIAHGEAAV
                     DGVFGWRASAWYRYDGGWVDRVDPTTGADLEHNIDRSHTLVLRLAAVWQAASSLTLTP
                     SMLYQNRQKHDASTYWPAYSQGAGEYNTGTPERIPDPDRYYLPALKLQWDLGKSQVIA
                     NASYYDRREQTGYQGTVYDLAYYQTLGWPTNPNTLGLSCGPSSVATVPPCDWYPLLDA
                     NGIHLPAGFTNEQTPNVITNTQQSYVGELRWQSSDDSSRWRWTVGTFWQLAKERSIEE
                     LKDVQIDPFFTYLYGVPATSLFFFTDPNGNVVSPYSCNGNGTYTAIP"
BASE COUNT          253 a          471 c          550 g          300 t
ORIGIN      
        1 caggaatcgc ggtgtaggtg ccgttaccgt tgcaggaata cggcgaaacg acgttgccgt
       61 tcgggtcggt gaagaagaac agcgaagtcg ccggcacgcc gtacaggtag gtgaagaacg
      121 gatcgatctg cacgtccttg agctcctcga tgctgcgctc cttggcgagc tgccagaacg
      181 tccccaccgt ccagcgccag cgcgagctgt catcgctcga ctgccagcgc agctcgccga
      241 catagctctg ctgggtgttg gtgatgacgt tcggcgtctg ctcgttggta aaacccgccg
      301 gcagatggat accgttggca tcgagcagcg gataccagtc gcaaggcggt acggttgcga
      361 ccgaggaggg tccgcacgag agaccgaggg tattcggatt ggtgggccat ccgagtgtct
      421 gatagtaggc gaggtcgtac acagtgccct ggtagccggt ctgctccctg cggtcgtagt
      481 acgaggcgtt ggcgatcacc tgactcttgc cgaggtccca ttgcagcttg agcgccggca
      541 ggtagtaccg gtcggggtcg ggaatgcgct ccggagtccc ggtgttgtat tccccggccc
      601 cctgcgagta cgcgggccag taggtcgacg cgtcgtgctt ctggcgattc tggtagagca
      661 tgctgggggt gagcgtgagg ctgctcgccg cttgccaaac cgcggcgagg cgcagtacca
      721 gcgtgtggga acggtcgata ttgtgctcga ggtccgcgcc ggtcgtcgga tcgacccggt
      781 ccacccagcc gccgtcgtag cgataccagg cgctggcgcg ccagccgaac actccgtcga
      841 cggccgcctc tccgtgcgct atgccgaact cgtagctcgg ctggccgtaa taggtgtagg
      901 cgagctcgct gcgcaggtag gtgctcgagc cggtgacgct cggctgggtc aggatgtagc
      961 gcacggtgcc gccctcggag cccgagccga acagcgtccc ctgcggacca cgcagcactt
     1021 cgacccgttg caggtcgaag gtcttcggca gcgtgtcgtc ggggttaaag ccgacgttgc
     1081 gcatctgaat cggcgtgtcg tcgatgtaga tgccggtggt gccggcgccg gcggacgatg
     1141 agatgccgcg gatcgagatg gcattggtgc cggagttgcc gatagacacg ccgggcagga
     1201 acctggcgat atcctggaag tccttgatgc cgcggtcgtc catggggtcc tgagtaaagg
     1261 ctgacacgct gattggcacc ttgctgagcg attcctcgcg ccggttcgcg gtcacgacca
     1321 cttcctgcag cttggtgcgc tcggtttcga caggtgcggc tgccgcggcc ggagtgggcc
     1381 cggcggccga ggcggtctcg tccgcacgca gcgcttcgag cggcagagcc gcggacagca
     1441 gcgcgataac gagcgagccg agcctggtcg tcgtaaggct tgcgttcagc atgtgtgtct
     1501 cccctcttct cccctggtta ggtctttatg attgcagcag cacggcggca tccgcgtccc
     1561 agctgacgaa tacc
//
LOCUS       DATLKL010000765         1979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_65709, whole genome shotgun sequence.
ACCESSION   DATLKL010000765 DATLKL010000000
VERSION     DATLKL010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65709"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..563
                     /gene="orn"
                     /locus_tag="VM713_10400"
     CDS             15..563
                     /gene="orn"
                     /locus_tag="VM713_10400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008048058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HVH84715.1"
                     /translation="MVWIDLEMTGLKADSDSIIEIATVVTDKELAVLADGPVIAIHQP
                     EEVLAGMDDWNKRQHGSSGLLTRVRGSLVSVAEAERRTLEFLTPLVTAGGSPMCGNSI
                     CQDRRFLARCMPELERFFHYRNLDVSTLKELARRWAPAVADTFVKQGTHLAHADIHES
                     IRELRRYRARLFAAAWQGTPAA"
     gene            539..949
                     /locus_tag="VM713_10405"
     CDS             539..949
                     /locus_tag="VM713_10405"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVH84716.1"
                     /translation="MAGHAGRVNLVTHRGGCHCGAVAFEVDAPARLTVSDCNCSICRM
                     SGYQHLIVPRARFRLVKGADSLSEYRFNTGTARHLFCRHCGVKSFYVPRSNPDGYSVN
                     ARCLDPATIEHIEMELFDDRERAAAEARIRDQSR"
     gene            complement(956..>1979)
                     /locus_tag="VM713_10410"
     CDS             complement(956..>1979)
                     /locus_tag="VM713_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HVH84717.1"
                     /translation="AEGDTGHVYEGLLDFEKKQIELDSLPKIPVKIMMNVGNPDRAFD
                     FAGIPNHGVGLARIEFIINRMIGVHPRALLEFERLDGELQQQIRVQTAGYSDPVGFYV
                     EKLAEGIAQIAAAFAPEPVIVRLSDFKSNEYANLIGGERYEPHEENPMLGFRGASRYT
                     DEGFRPCFELECRALKRVRETMGLTNVAVMVPFVRSVAEARQVTQLLAANGLKRGADG
                     LRVIMMCELPTNALLAEEYLDHFDGMSIGSNDMTQLTLGVDRDSAIVARLFDERDAAV
                     KRLLTMAISACRKRGKYVGICGQGPSDYPELARWLVDQGIESMSLNPDTVVETWLFLA
                     QQSKPG"
BASE COUNT          318 a          664 c          659 g          338 t
ORIGIN      
        1 cgagcgccga ctacctggtc tggatcgatc tcgagatgac cggcctgaaa gccgactcgg
       61 actcgatcat cgagatcgcc accgtggtca ccgacaagga gctggcggta ctcgccgacg
      121 gcccggtgat cgccattcat cagcccgagg aggtgctcgc gggcatggat gactggaaca
      181 agcgccagca cgggtcctct ggcctgctga cgcgcgtgcg cgggagcctc gtgagcgtcg
      241 ccgaggccga gcggcgcacc ctcgagttcc tcacgccgct cgtcaccgcc ggcggctcgc
      301 ccatgtgcgg taacagcatc tgccaggacc gccgcttcct cgcgcgctgc atgcccgagc
      361 tcgagcgctt cttccactac cgcaatctcg acgtgagcac gctcaaggag ctggcgcgac
      421 gctgggcgcc ggcggtcgcc gacactttcg tcaagcaggg cacgcacctg gcgcacgccg
      481 acattcacga gtcgatccgc gagctgcgtc gctatcgcgc acgtctgttc gcggcggcgt
      541 ggcagggcac gccggccgcg tgaatctggt gacgcaccgc ggcggctgcc actgtggggc
      601 ggtcgccttc gaagtggacg cgccggcacg tctgacggtg agcgactgca actgctcgat
      661 ctgccgcatg agcggctatc agcatctgat cgtgccgcgg gcgcgctttc gcctggttaa
      721 gggggcggac agcctcagcg aatatcgctt caacaccggc acggcgcgcc acctcttctg
      781 ccgccactgc ggcgtgaagt ccttctacgt gccacgctcg aatcctgacg gctacagcgt
      841 caatgcgcgc tgcctcgatc cggctacgat cgagcacatc gagatggagc tcttcgacga
      901 ccgcgagcgc gccgccgccg aggcgcgcat ccgcgaccag tctcgttagc gatttctacc
      961 cgggcttgga ctgctgcgcg aggaacagcc aggtctcgac cacggtatcg ggattgaggg
     1021 acatgctctc gatgccctga tcgaccagcc agcgcgcgag ctcggggtaa tccgaaggcc
     1081 cctgcccgca gatgccgacg tacttgccgc gcttgcggca ggcgctgatc gccatggtga
     1141 gcaagcgctt gacagccgcg tcccgctcat cgaacagcct cgcgacgatc gccgagtcgc
     1201 gatcgacgcc gagcgtcagc tgcgtcatgt cgttcgagcc gatcgacatg ccgtcgaagt
     1261 gatcgagata ctcctcagcg agcagcgcgt tggtcgggag ctcgcacatc atgatgaccc
     1321 gcaggccgtc ggcgccgcgc ttgaggccgt tggcggccag cagctgcgtg acctggcggg
     1381 cctcggcgac gctgcgcacg aacggtacca tcactgcgac gttggtgagt cccatggtct
     1441 cgcgcacccg cttgagcgcc cgacactcga gctcgaagca ggggcggaag ccctcgtcgg
     1501 tgtaacgcga ggcgccgcgg aagcccagca tcgggttctc ctcgtgcggc tcgtagcgct
     1561 cgccgccgat caggttggcg tactcgttcg acttgaagtc ggagaggcgc acgatgaccg
     1621 gctcgggggc gaaggcggcg gcgatctgcg cgatgccctc ggcgagcttc tcgacgtaga
     1681 agcccaccgg gtcggagtag ccggccgtct gtacgcggat ctgctgctgc agctcgccgt
     1741 cgaggcgctc gaactcgagc agcgccctgg ggtgcacgcc gatcatgcgg ttgatgatga
     1801 actcgatgcg cgccagtccc acgccgtgat tggggatgcc agcgaagtcg aaggcgcggt
     1861 cggggttgcc gacgttcatc atgatcttga ctgggatctt cggcagactg tcgagctcga
     1921 tctgcttctt ctcgaaatcg agcaggccct cgtagacgtg cccggtgtca ccttcggcg
//
LOCUS       DATLKL010000766         1531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_223111, whole genome shotgun sequence.
ACCESSION   DATLKL010000766 DATLKL010000000
VERSION     DATLKL010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223111"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..300)
                     /locus_tag="VM713_10415"
     CDS             complement(70..300)
                     /locus_tag="VM713_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84718.1"
                     /translation="MNLMRVTVYLGENGAGRSSTYAIAASSPREAAILMSDHLGLRYR
                     WERIDVELLFGERVAGPPRVLGPVGGEVKARS"
     gene            complement(456..701)
                     /locus_tag="VM713_10420"
     CDS             complement(456..701)
                     /locus_tag="VM713_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84719.1"
                     /translation="MTGMRMLGDYPYDPVRVACRRCDWRGQYRRSSLIALYGENAALP
                     YVLRQLVQDCPKRYRFGNEACGAYFPDLLERPQPRGR"
     gene            1088..1531
                     /locus_tag="VM713_10425"
                     /pseudo
     CDS             1088..1531
                     /locus_tag="VM713_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019331326.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          258 a          491 c          519 g          263 t
ORIGIN      
        1 aggcgctggg gacgaagccg cccggcccgg tgcattgacg cgtgggccgg atcgcgccag
       61 agacgacgct cagctccgcg ccttcacctc gccgccaacc ggcccgagga ctcggggcgg
      121 ccccgcgacg cgctcgccga agagtagctc gacatcgatc cgctcccacc ggtaacgaag
      181 accgagatgg tcagacatga gaatcgccgc ctcgcggggg ctgctggcgg cgattgcata
      241 cgtcgagctc ctcccggcgc cgttctcgcc aaggtagacg gtgactcgca tcaggttcac
      301 ggtggctcgc ctgattgagt gtgtttccga tcgcacactc ttatcgcgca tcatgtttcg
      361 cgtcggttaa cggccatcgc ggcgtgggcg gcagcccgtc gcacggctcg cctaaccgcc
      421 cccgcggcgg gatggcctgc cgccggcgcg cggccctatc gccccctcgg ctgcgggcgc
      481 tcgaggaggt ctgggaaata ggcaccgcag gcctcgttgc cgaacctgta gcgcttcggg
      541 caatcctgga cgagctgacg caacacgtac ggcagcgccg cattctcgcc gtagagcgcg
      601 attaggctcg agcgccggta ctgcccgcgc cagtcgcacc ggcggcaggc gacgcggacc
      661 gggtcgtacg ggtagtcgcc aagcatgcgc atgccggtca tggcagccgc atcttacacc
      721 acgctcggac agatcggacg ccttcacgcg cgctagtgga cagggcgcaa gtcctccgtg
      781 ccgatgcgcg caaggtatcc cttggcgagg ctgtcgatga ccatgatgtt gatggggaac
      841 gtgaagccgc ctcgaccagc ttctcggcga cgatgcggcg ctcgccgcgc tcggcctcat
      901 tttggctaat gtccggaaat ggcactcccc ggaagaggcg atcggcatcg gcaaagtctg
      961 gtaattggac ctgagcggac ctagcgcgga cgtgccccga cagccgccta tgaccctggt
     1021 tgtgtaaaaa cgttccacct ggcgtgactc gtgcgatacg attccaccgt gatttgcggg
     1081 ggatcggatg aagcgcttcg tcgagggagt ggatcggggc caatcgacgc tctttccgga
     1141 atgtctggag gactggatcg acgaggatga tccagttcgg gtgatcgacg cattcgtcga
     1201 tgccctcgat ctggccgggc tcgggttcga gggcgtcgag ccggcggctc gaacgcgagg
     1261 ccggccgcaa cgtcgaagtg atgtggcttc tgggccgcct cgtccccgac cataagacgg
     1321 tggccgattt ccggaaggac aacggtcgtg ccatccgcaa ggtttgcgcg cgcttcgtcg
     1381 aactctgccg cgagatgggt ctgcttgtga cggcgagcgt cgccatcgat ggcagcaagt
     1441 tcaaggccgt gaacaatcgc gatcggaact tcacgcgcgc gaaggtggag cggcgccggg
     1501 cccagctcga ggagagcgtc gccagctatc t
//
LOCUS       DATLKL010000767         1861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_54309, whole genome shotgun sequence.
ACCESSION   DATLKL010000767 DATLKL010000000
VERSION     DATLKL010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54309"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..638
                     /gene="moaC"
                     /locus_tag="VM713_10430"
     CDS             159..638
                     /gene="moaC"
                     /locus_tag="VM713_10430"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HVH84720.1"
                     /translation="MARLTHFDAAGNAVMVDVSAKPETARIAAAKGSVIMQPTTLARI
                     MAGRVEKGDVLAVARLAGIMGAKRTAELIPLCHPLGVTSVTVDLVCDAARHAVDITAT
                     CKITGRTGVEMEALTAVAVAALTIYDMCKAVDRGMHLTDIRLVEKSGGKSGEYRAKR"
     gene            635..1849
                     /gene="glp"
                     /locus_tag="VM713_10435"
     CDS             635..1849
                     /gene="glp"
                     /locus_tag="VM713_10435"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HVH84721.1"
                     /translation="MISVEEALRRVVEAFSPLAVEQVGLAEGLGRVLAEDVIARITQP
                     PKAVSAMDGYAVRASDVQKVPVTLRCVGAAPAGVPYDGRLGPGQAIRIFTGAPVPAGT
                     DAIVIQEDTKSEGDRVVVLEAPQPGRYIRPAGLDFQRGEVGITAGRRLGPRDIALAAA
                     MNVPWLSVRRKPRVAILATGNEIVMPGDPLGPDQIVSSNALALAAAIVAWGGEPLNLG
                     IAKDTKESLQALAAGARGADLLITTGGASVGEHDLVQSALSERGLKVDFWQIAMRPGK
                     PLIFGRLGDTPLLGLPGNPVSSMVCAIVYLKPVLERMLGLAAVERPQATARLGRDIGA
                     NDRRQDYLRSRLERARDGELVVTPFDVQDSSMLSRLAQADCLLIRPPLAPPAKANDRV
                     PIIVLDAAFASS"
BASE COUNT          293 a          645 c          608 g          315 t
ORIGIN      
        1 ggcctcaaga cggccgagga tctcgcgcga atggcgcggg ccggcgtgca ctgcttcctt
       61 gtcggcgaat cgttgatggc cgggcccgat gtggcggcgg caacgcgcgc gttgcttacc
      121 cggccggcag ccccacgccg cgcccggggc tagcccctat ggcccggctc actcatttcg
      181 atgctgcggg caacgccgtt atggtggacg tctcggcaaa gcccgagacg gcgcgcatcg
      241 ccgccgcaaa gggctcggtc atcatgcagc cgacgaccct ggcgcgcatc atggccggtc
      301 gcgtcgagaa gggggacgtc ctcgcggtcg cgcgactggc gggcatcatg ggcgccaagc
      361 gcaccgccga actcatcccc ctctgccatc ccctcggtgt cacctcggtc acggtcgatc
      421 tcgtctgcga cgccgcgcgg catgctgtgg atattacggc cacctgcaag atcaccggcc
      481 gcactggagt cgagatggag gccctcaccg cggtcgcggt agccgcgctg accatctacg
      541 acatgtgcaa ggcggtcgac cgcggcatgc acctgaccga cattcgcctc gtggagaaaa
      601 gcggaggcaa atcaggagaa tacagggcga agcgatgatt tctgtcgagg aagcgctacg
      661 ccgcgtggtc gaggccttca gcccgcttgc agtcgagcag gtcggcttgg ccgaaggact
      721 cgggcgcgtc ctcgccgaag atgtcattgc gcgcatcacg cagccgccca aggcggtctc
      781 tgccatggat ggctatgcgg tgcgcgcgtc cgacgttcaa aaggtgccgg tcaccctccg
      841 ttgcgtcggt gcagcaccgg caggtgttcc ctatgacggg cggctcggcc cggggcaagc
      901 gatccggatt ttcacgggtg ccccggttcc ggcaggtacg gatgccatcg tcatccagga
      961 ggacaccaag tccgagggcg accgtgtcgt cgtgctggaa gcgccccagc cgggacgcta
     1021 catccgtccc gccggtctcg atttccagcg cggcgaggtc gggatcacag cgggtaggcg
     1081 ccttgggccg cgcgatatcg cgctcgccgc ggcgatgaac gtgccgtggc tcagcgtgcg
     1141 ccgcaagccg cgtgtcgcca ttctcgcgac tggcaatgaa atcgtcatgc cgggcgatcc
     1201 tctcggtccc gaccagatcg tgagttccaa cgcgctcgcc ctcgcagcgg caatcgtcgc
     1261 ttggggcggc gagcctctta acctcggcat cgcaaaggac actaaggaat cgctccaagc
     1321 cttggccgcg ggcgcgcgcg gggccgacct cctgatcacc acaggcggtg cctcggttgg
     1381 agagcacgat ctggtgcaga gcgccctcag cgagcgcggg ctgaaggtcg atttctggca
     1441 gattgccatg cggcccggta agcccctcat cttcggccgg ttgggcgaca cgccactgct
     1501 cggcctgccg ggaaaccctg tttcctccat ggtctgcgcc atcgtctatc tgaagcccgt
     1561 gctcgagcgc atgctcggcc tcgccgccgt cgaaaggccg caagcaaccg cacggctcgg
     1621 ccgggatatc ggcgcgaatg accgccgcca ggattatttg cgttcgcgcc tcgagcgggc
     1681 gcgtgatggc gaactcgtcg tcaccccctt cgacgtgcag gacagctcga tgctatcccg
     1741 cctcgcccag gccgactgtt tgttgatccg cccgcccctc gccccgcccg ccaaggccaa
     1801 cgaccgggtc cccatcatcg ttctagacgc cgccttcgca tcatcttgag ccgggcccat
     1861 t
//
LOCUS       DATLKL010000768         1802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_200965, whole genome shotgun sequence.
ACCESSION   DATLKL010000768 DATLKL010000000
VERSION     DATLKL010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200965"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VM713_10440"
     CDS             <1..378
                     /locus_tag="VM713_10440"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVH84722.1"
                     /translation="LLLEEALADADRGGRHLDEAALFWAQALGRPVDPQHPGTRGNYR
                     MLETPPDEPIVQIQRVEHESRVHIDIETDDIPAEVARLEKLGARVAERLPRWVVMQAP
                     TGQRFCVVRVQRPGFPKNANRWE"
     gene            complement(399..1013)
                     /gene="folE"
                     /locus_tag="VM713_10445"
     CDS             complement(399..1013)
                     /gene="folE"
                     /locus_tag="VM713_10445"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HVH84723.1"
                     /translation="MGEPSMARERGKISRRAAEDAVRVLLRWAGDDPGREGLRDTPQR
                     VVDAYRDWFSGYKSDPAAYLRRTFEEVAGYDEMIVLRDISFESHCEHHIAPMIGRVHI
                     GYLPANKVVGISKLARVVDGYARRLQVQEKLTAQIADCIADVLKPRGVGVVVDAAHEC
                     MTTRGVHKRSVSMVTSRMIGSFRSDARTRSEFLTFIGISGAREV"
     gene            complement(1056..1802)
                     /locus_tag="VM713_10450"
                     /pseudo
     CDS             complement(1056..1802)
                     /locus_tag="VM713_10450"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
BASE COUNT          300 a          622 c          640 g          240 t
ORIGIN      
        1 ctgctccttg aggaagcgct tgcggatgcg gatcgaggtg ggcgtcacct cgacgaggca
       61 gcgctcttct gggcgcaggc actcggccgg ccggtcgacc cacaacatcc ggggacgcgc
      121 ggcaattacc gcatgctcga gactccgccc gatgagccca tcgtgcagat ccagcgcgtg
      181 gagcacgaga gtcgcgtcca catcgatatc gagactgacg atatcccggc ggaagtggcg
      241 cgcctggaga agctcggtgc cagggtggcc gagcggctac cgcgctgggt cgtgatgcag
      301 gcgcctacgg gccagcgctt ctgtgtcgta cgggtgcagc gacccggctt cccgaagaac
      361 gccaaccgct gggagtgagc gcagcgcacg gcgcggcgct acacctcgcg cgcgcccgag
      421 atgccgatga aggtcaggaa ctcggagcgc gtgcgcgcat cgctccgaaa ggagccgatc
      481 atgcggctcg tgaccatcga gacgctgcgc ttgtgcacgc cgcgggtggt catgcactcg
      541 tgcgctgcgt ccaccaccac gccgacgccg cgtggcttca gcacgtccgc gatgcagtcg
      601 gcgatctgcg cggtgagctt ctcctgcacc tgcagccgcc gcgcgtagcc gtccaccacc
      661 cgggccagct tgctgatgcc gaccaccttg ttggcgggca ggtagccgat gtgcacccgg
      721 ccgatcatgg gtgcgatgtg gtgctcgcaa tgcgactcga aggagatgtc gcgcagcacg
      781 atcatctcgt cgtagccggc gacctcctcg aaagtccggc gcaggtaggc ggcggggtcg
      841 ctcttgtagc cggagaacca gtcgcggtag gcatcgacga cgcgctgcgg cgtgtcgcgc
      901 agcccctcgc ggcccgggtc gtcgcccgcc cagcgcagca ggacgcgcac ggcatcctcc
      961 gccgcgcgac gcgaaatctt ccctcgctcc ctggccatgc tcggctcccc catctgctgg
     1021 cgcccccggg gcgccggtcg cgggagttta ggggttcagt gcgcgaagtg cagcagcgtg
     1081 agcgtgagcg tcaggcttga ggccccgacg aggctcgcta ctaccagtcc cgccgcgagc
     1141 ggccgccagc cgatgcgcca catgtcggtg aaggagaccg aaagcccgac cgcggtcatg
     1201 ccgcaggcaa gaaagaggtc ggaggccgtg tagccggtgt gcacgacggc ctgccagagc
     1261 gcactcccct cgccgagcag cgtgtcgaag ccggtgcgca ccacgatgaa ggcgagaaag
     1321 gcgacgacga acatcggcac gagctgcgcg tgaggcgcga gcccgtgatg ggcctcgggc
     1381 gcatggtcgt cggcgccgcc gcgcgcctcg tggcggcgcg ccagccaggc ggcgagcggc
     1441 accagcgccg cgatcgagag attgcgcagg agcttggcga cgctcgccgc ggcgagcgcg
     1501 gccggagctg ccgcctgctg cgactacatg agagctgcgc cgatcacctg cgaggtgtcg
     1561 tggatcgccg tgccgagaaa gatcccggcg tgtaccgggg aggcggcaaa gaaatgcccc
     1621 gcgacccacg gatagcagag catcgcggcg cagccgaaca gcaccacgca ggtgacggcg
     1681 aaagcggtct ccgcatgacg cgcccggatc accggcgaca tcgcgaccac cgcggtgcag
     1741 ccgcacaccg ccgtgccgat cgccagcagc gctccgagcc gcgcgccgac tccgaagagg
     1801 cg
//
LOCUS       DATLKL010000769         1584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_43124, whole genome shotgun sequence.
ACCESSION   DATLKL010000769 DATLKL010000000
VERSION     DATLKL010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43124"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VM713_10455"
     CDS             <1..525
                     /locus_tag="VM713_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84724.1"
                     /translation="KGRELNPYRAGFLQLVAVADTDAQAEAKYARHVEYFYQKCLHLP
                     LHLLSPAGNQDYRSLVANLANPVRRIDDMKSLRYGDFVERGYVIAGSPATVRDRLREE
                     VVKELRVGNLMVLLQIGSMPHQLALENIDLFARKVLPEFRDEWNDDGWVNYWWPEQLR
                     DRVGESVPAAVGGQ"
     gene            528..1325
                     /locus_tag="VM713_10460"
     CDS             528..1325
                     /locus_tag="VM713_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVH84725.1"
                     /translation="MDREQTIAVWDGCVRMRVLSAGAGPALVFLHGPWGLAWDSFLDD
                     LARSFTVYAPEHPGTTPEAHDDIYRLDGLWDLILCHDELLTALNIEEALVVGHSFGGM
                     VACELAAAYPQRVRRLALIDPIGFWRDTDPIVNWVKLSPAELPNYIFRNPAVEAAQRM
                     FGPSAPPDAAIAARVRLMWAMGATSKFIWPIPDKGLKKRIHRVSAPTLVVWGADDRLV
                     PSVYADEFSKRLASARVHIITGAGHAPHLEHPAAVAGLVRDFVTATP"
BASE COUNT          269 a          489 c          540 g          286 t
ORIGIN      
        1 aagggacgcg aactgaaccc ataccgggcg ggattcctcc agctcgtcgc cgtcgctgac
       61 accgatgcgc aggccgaggc gaagtatgcc cggcacgtcg agtacttcta tcaaaaatgc
      121 ctgcacttgc cgctccacct gctctcgccg gcgggcaatc aggattatcg cagcctggtg
      181 gcgaatctcg caaacccggt ccgccgcatc gacgatatga agtcgcttcg ttatggcgac
      241 ttcgtggagc ggggctacgt gatcgcagga agcccggcga ccgtacgtga tcgccttagg
      301 gaagaggtcg tcaaggaact gcgcgttggc aatctgatgg tgctgcttca gatcggctcg
      361 atgccacatc agctggcact ggagaatatc gaccttttcg cccgcaaggt gttgcccgag
      421 ttccgagacg agtggaacga cgatggctgg gtcaactact ggtggccgga gcagctgcgc
      481 gaccgcgtgg gcgagtccgt cccggcagcc gtgggagggc aataggcatg gaccgcgagc
      541 agacgatcgc cgtctgggac ggctgcgttc gcatgcgcgt gctatcggcg ggtgcagggc
      601 cggcgctggt ctttctccac ggcccgtggg ggctcgcgtg ggattcgttt ctcgacgatc
      661 tggctcggag cttcacggtt tatgcgcctg agcatccggg gacaacgccg gaggcgcacg
      721 acgacatcta tcggctcgac ggcctctggg atctgatcct ctgccacgac gagctgctga
      781 cggctctgaa catcgaagaa gcgttggtcg tcgggcattc gttcggcggc atggtcgcgt
      841 gcgagctggc ggcggcgtac ccgcagcgcg tgcgccgcct ggccctcatc gatccgatcg
      901 gcttctggcg tgacaccgat ccgatcgtga actgggtgaa gctgagcccg gcggagctgc
      961 cgaactatat ctttcgcaat cccgccgtcg aggcggcaca gcggatgttt gggccgtcgg
     1021 caccgcccga cgccgcgatc gccgcacgcg tacgcctgat gtgggcaatg ggcgcgacga
     1081 gcaagttcat ctggccgatc ccggacaagg ggttgaagaa gcgaatccat cgcgtctctg
     1141 cgccaacgct cgtcgtctgg ggtgcggacg accggctcgt gccgtcagta tacgcggacg
     1201 agttctcgaa acgcctcgcc agtgcgcggg tccatatcat cacgggcgcc ggtcatgcgc
     1261 ctcacctcga gcacccggca gcggtagcag gtctcgtccg cgactttgtg actgccacgc
     1321 cgtagctgta gcggtttgca ctatctgtgt cgacatccca agccgcctcg ttcgcctggc
     1381 gtcgatgaat gaagggaggg gagcaatgtc taaggataat tcccgggcaa ggtcggccga
     1441 cgtacatgac agctgccagg cggcgccagc ggcatcgcgg cgcggactgc tggcggcggg
     1501 cgccgggctt gccggcggcg ccatggcgca actgtttacg acgggagccg tggccgccgg
     1561 agcgagtgcc acgctcgagc gaca
//
LOCUS       DATLKL010000770         1603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_20407, whole genome shotgun sequence.
ACCESSION   DATLKL010000770 DATLKL010000000
VERSION     DATLKL010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20407"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VM713_10465"
     CDS             <1..916
                     /locus_tag="VM713_10465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HVH84726.1"
                     /translation="ARHGRLVYEEYFDGGADTLRDTRSATKSITDILVGIAIDEKKLR
                     GVDARILELLPDRASRLQNPDARKSRISIEDLLTMSSPLECDDWNDASRGNEERMYVI
                     EDWTQFILDLPIRGRMHVGETVPAPPFGRYFSYCTGGVFVLSEVLTRTTGMRTDRYAQ
                     ARLFEPLGITKVSWVFSPLGMPQTGGGLGLTARDLLKIAQLYLDGGRWTGKQIVSEAW
                     VRASTTAHAQVDETTNYGYLWWLKSFGPEGKAHSAFLMNGNGGNKVVAIPDLDLTVVI
                     TSTNYNTRGMHEQTDKILVDYVLAAVER"
     gene            complement(939..1334)
                     /locus_tag="VM713_10470"
     CDS             complement(939..1334)
                     /locus_tag="VM713_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84727.1"
                     /translation="MDHTSRLIGLAFVVAWTIFRLVRYLRMSGAKPRPRTAAVAAPTA
                     TASPIEPAGTTAGFARNVLAAAVFIPGTVVIWALLFMVPAIEELPPLLRMIAGVLATL
                     YLIHLARSVAARSGGTGRRGDAADNNPIK"
     gene            complement(1338..>1603)
                     /locus_tag="VM713_10475"
     CDS             complement(1338..>1603)
                     /locus_tag="VM713_10475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HVH84728.1"
                     /translation="LLKGEGSAVPGTRLLLSPGGTATLDRVPRPDGGVIVLVGPEGGL
                     TDEEEQAAIAAGFTALRLGPRVLRTETAAIAALTLLQRQFGDL"
BASE COUNT          288 a          547 c          534 g          234 t
ORIGIN      
        1 cgcccggcac ggccggctgg tctatgagga gtatttcgac gggggcgccg acacgctgcg
       61 cgacacgcgc tcggccacca agagcatcac ggacatcctg gtcggcatcg cgatcgacga
      121 aaagaagctg cgcggcgtcg atgcgcgcat tctcgagctg ctgcccgacc gcgcgagcag
      181 gctgcagaac cccgacgcgc gcaagagccg catcagcatc gaagacctcc tcacgatgag
      241 ctcgccgctc gagtgcgacg actggaacga cgcctcgcgc ggcaatgagg agcgcatgta
      301 cgtcatcgag gattggacgc agttcatcct cgatctgccg atccgcggcc gcatgcacgt
      361 gggcgagacc gtgccggcgc cgccattcgg ccgctatttc agctattgca ccggcggcgt
      421 cttcgtcctg agcgaggtgc tcacccggac gaccggaatg cgtaccgatc gttacgcgca
      481 ggcgcgtctg ttcgagccgc tcggcatcac gaaggtatcg tgggtcttct cgcccctcgg
      541 catgccgcag acgggcgggg gtctcgggct caccgcacgc gacctgctga agatcgcgca
      601 gctctacctc gacggcggcc ggtggacggg caagcagatc gtcagcgagg cctgggtcag
      661 ggcctcgacc accgcgcatg ctcaggtcga tgagaccacc aactacggct atctctggtg
      721 gctcaagtca ttcgggcccg aggggaaggc acattccgct ttcttgatga acggtaacgg
      781 cggcaacaag gtcgtggcga tcccggacct cgacctgaca gtggtcatca ccagcaccaa
      841 ctacaacacc cgcggcatgc acgagcagac cgacaagatc ctcgtcgact acgtgctcgc
      901 tgcagtggag agatgagcgc acgagaggcc ggggaccctc acttgatcgg attgttgtcg
      961 gcggcgtcac cgcgtcggcc cgtaccgccg gagcgggcag ccacgctcct ggcaaggtgg
     1021 atgagataga gcgtcgcgag cacgccggcg atcatccgca gcagtggcgg tagctcctcg
     1081 atcgcaggga ccatgaagag caacgcccag atgaccacgg tcccgggaat gaacacagcg
     1141 gctgccagaa cattgcgcgc gaaccctgca gtggtgcccg ccggctcgat cggcgaggct
     1201 gtggcagtcg gtgccgcaac ggcggcagtg cgcggccgag gcttggcgcc ggacatccgc
     1261 aggtagcgca ccagtcgaaa aatcgtccag gccacgacaa acgcgagccc gatcagccgc
     1321 gaggtatggt ccatcggcta caggtctccg aactgccgct gcagcagcgt gagggccgcg
     1381 atcgccgccg tctcggtgcg cagcacgcgc ggtccgaggc gcagcgccgt gaaccccgcc
     1441 gcgatcgctg cctgctcctc ctcgtccgtg agtccgccct cgggcccgac tagcacgatg
     1501 acgccgccgt ccgggcgcgg cacgcggtcg agcgtcgcgg tgccgccggg cgacagcagc
     1561 agtcgcgtgc ccgggaccgc gctcccctca cccttgagga gct
//
LOCUS       DATLKL010000771         1939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_122179, whole genome shotgun sequence.
ACCESSION   DATLKL010000771 DATLKL010000000
VERSION     DATLKL010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122179"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VM713_10480"
     CDS             complement(<1..410)
                     /locus_tag="VM713_10480"
                     /inference="COORDINATES: protein motif:HMM:NF020858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84729.1"
                     /translation="MHPNDPGLRSFADVPGDSPFPIQNLPYGMFSTPNHAASRAGVAI
                     GDYVLDLAALENAGLLRIVPGGSRIFDRPALNAFIALGPATWTRVRARLSELLRHDNP
                     ELRDNAALRRRAIVPLTRVVLHLPVEIPGYTDFYS"
     gene            complement(426..1772)
                     /gene="hmgA"
                     /locus_tag="VM713_10485"
     CDS             complement(426..1772)
                     /gene="hmgA"
                     /locus_tag="VM713_10485"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HVH84730.1"
                     /translation="MSATSAKRGLKRAADRAQTLAYQSGFGNEFATEALAGALPVGRN
                     SPQRAPYGLYAEQFSGTSFTTPRAANRRSWLYRVRPAVLHRPFARIDNRLIASRFDEI
                     PTTPNQLRWDPLPIPAQPTDFVEGLATMAGNGDPATQSGCAIHVYTANRSMTDRYFYS
                     ADGELLIVPQQGRLRLATELGLIEAEPLEIVVIPRGLRFRVELPDGAARGYVCENHGA
                     LLRLPDLGPIGANGLANPRDFVTPVAWYEDREGSFELVTKFMGNLWSAAIGHSPLDVV
                     AWHGNYAPYKYDLRRFNAIGSVSYDHPDPSIFLVLQSPGDRPGVADIEFVIFPPRWLV
                     MEGSFRPPWFHRNVASEFMGLIHGAYDAKAEGFAPGGSSLHNCMSGHGPDADTVERAT
                     AADTTKPARIEDTMAFMFETRCVIRPTRFALESPQLQPEYFRCWQGLQKHFDPAAR"
BASE COUNT          347 a          626 c          657 g          309 t
ORIGIN      
        1 gagtagaaat ccgtgtagcc tgggatttcc accggaagat ggagcaccac acgcgtcaac
       61 gggacgatgg cgcgccgccg cagcgcggcg ttatcccgca actcgggatt atcgtgacgc
      121 agcagctcgc tcaatcttgc ccggaccctg gtccacgtcg caggtccgag cgcgatgaac
      181 gcgttgagcg ccggacggtc gaatatgcgg ctccctccgg gaacgatcct caggagtccg
      241 gcattctcga gcgccgcgag atcaaggacg tagtcgccga tcgccacgcc cgcgcgcgac
      301 gccgcatggt tgggcgtcga gaacatgccg tagggcaggt tctggatggg aaagggcgaa
      361 tcgcccggga catcggcgaa cgatctcagg ccgggatcat tcggatgcat gcccttcgga
      421 ccctctcatc gggcggccgg atcgaagtgc ttctgcagcc cctgccaaca acggaaatat
      481 tccggctgca gctgcggcga ttcgagcgcg aagcgagtag gacggatcac gcagcgcgtc
      541 tcgaacatga aggccatggt gtcttcgatg cgagcgggct tcgtggtgtc ggccgcggtg
      601 gcccgctcga cggtatcggc atccggcccg tgaccgctca tgcaattgtg gaggctggag
      661 ccgccggggg cgaagccctc cgccttggcg tcgtaggcgc catggatcag ccccatgaac
      721 tcgctcgcta cattgcggtg gaaccagggt ggccggaacg agccctccat gaccagccac
      781 cgcggcggga agatcacgaa ctcgatgtcg gccacgcccg gcctgtcgcc gggcgactga
      841 agcaccagga atatcgacgg gtccgggtga tcgtagctga cggagccgat ggcgttgaac
      901 cgtcggagat cgtacttgta gggcgcataa ttgccatgcc atgcgaccac gtcgagcggc
      961 gaatggccga tcgcagccga ccagaggttg cccatgaatt ttgtcaccag ctcgaagctg
     1021 ccctcgcggt cttcgtacca cgcgaccggc gtcacgaagt cgcgcgggtt cgcgagccca
     1081 ttggcgccga tcggcccgag atccggaagg cggaggagcg cgccgtggtt ctcgcacaca
     1141 tagccccgag ccgcgccatc cggcagttcg acccgaaagc gcagcccgcg cggaatgacg
     1201 acgatctcca gcggctcggc ctcgatgagg ccgagttcgg ttgccaggcg aagcctgccc
     1261 tgttgcggca cgatcaggag ctcgccatcc gcgctgtaga agtagcggtc ggtcatcgac
     1321 cggttggcgg tgtaaacgtg aatggcgcag ccactctgcg tggcggggtc accgttgccc
     1381 gccatggtcg cgaggccctc gacaaagtcg gtcggctggg ccgggatggg aagcggatcc
     1441 cagcgcagct gattgggcgt ggtgggtatc tcgtcgaagc ggctcgcaat cagtcgatta
     1501 tcgatccggg caaacggacg gtgcaggacc gcggggcgga cgcgatagag ccacgaccgt
     1561 cggttcgccg cacgcggcgt cgtgaacgac gtaccggaaa actgctcggc ataaaggccg
     1621 tatggcgcgc gctggggcga gttccggccg accggcagcg cgccggcaag ggcctccgtc
     1681 gcaaattcgt tgccaaagcc ggattggtag gccagcgtct gcgcccgatc cgccgctcgt
     1741 ttcaatccgc gcttcgccga tgtcgcgctc atgggtcacc ttccgctctc gattgcgcgc
     1801 gcgagagccg ttcgctccct ggcgcgccgc accccgaaga ctacgcgcgc acaagccagg
     1861 acacgcccgg ccatcgcggc aaaatgccga ctcgccggcc gcttgaatcc cggcggttca
     1921 ctccggcgaa cgggaccga
//
LOCUS       DATLKL010000772         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_54380, whole genome shotgun sequence.
ACCESSION   DATLKL010000772 DATLKL010000000
VERSION     DATLKL010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54380"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VM713_10490"
     CDS             complement(<1..517)
                     /locus_tag="VM713_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84731.1"
                     /translation="MGPNRLGLSVALITLVAVAGCGGSGSDSGSSPSPPPPPDLSGVW
                     AGSWQGVDPAIGPVSGTWEATLSGTTTGVSGSGTLFGDVDCMDGTVAGAVSGNTFPGT
                     LDRPPCGTNSWSLLALSTPDETASGGWNQQSSNAQGTFIGARIARPGGPRIEFVSPPG
                     GVPGTIVTIVGS"
     gene            complement(523..1089)
                     /locus_tag="VM713_10495"
     CDS             complement(523..1089)
                     /locus_tag="VM713_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84732.1"
                     /translation="MHSTRLLARCTRRALHSCVVVAALTAAGCAGTAPRPEFSHTSTV
                     VRLAAADAVEVSVTAAGNAQVLPEEQTRLAQKIKLRIDNRKLAHQLPGDARNFELDLQ
                     LSRYQRGNAFARAMLAGLGQIHIEGKVRLYQMPAHELVTEFDLAKTFAWGGIYGASTS
                     IEDIENSFADGVAAAITDEQPAKPKQGT"
     gene            complement(1219..2154)
                     /locus_tag="VM713_10500"
     CDS             complement(1219..2154)
                     /locus_tag="VM713_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVH84733.1"
                     /translation="MTKSARPELTALLALFAGALAIGSSGIFVRVAETGAVASAFWRG
                     ALALPPLALWAWLEKRAPPAARAGARGSLLDAAFLWAGVFFAGDIALYHLSLSRTSVG
                     AATLEANTAPIVVTLLVWVVWGERPRLGFLLATLLAFGGLLLIVSPKLTQGGHALGGD
                     GLALGAAWLYAAYILAVARLRARHGTGTVMFASTLIFSVLLLPVPFLLGEKFLPDTPR
                     GWLLLVALALSAQALGQSLIAYALAHLRPTFGSLGLYLQVIAAAVYAWLLLGERLRPV
                     QLAGGVIVLTGIALARASRATRAASPASEPAAASS"
BASE COUNT          359 a          767 c          787 g          285 t
ORIGIN      
        1 agctgccgac gatcgtgacg atcgtacctg gcacgccgcc cggcggcgac acgaattcga
       61 tgcggggtcc gcccggccgg gcgatccgcg cgccgatgaa ggtcccctgc gcgttgctgc
      121 tctgttgatt ccaaccgccg ctcgcagtct cgtcgggcgt actcaaagcc aatagactcc
      181 aggagttcgt tccgcacggt gggcgatcga gcgtcccggg aaaggtgttg ccgctcacgg
      241 cgccggcgac cgtgccgtcc atgcagtcga catcgccgaa cagagttccg ctaccggaga
      301 cgccggtagt agtcccggag agggtcgcct cccaggtccc cgagaccggc ccgatggcgg
      361 gatcgactcc ctgccagctg cccgcccaga ctccggacag atccggaggc ggcggaggag
      421 aaggcgagct accgctatcg ctaccgctgc cgccgcagcc cgccactgcg accagggtga
      481 tcagagcgac cgacaagccc aggcgattgg ggcccatgac agttacgtcc cctgcttcgg
      541 cttcgcgggc tgctcatccg tgatggcggc cgcgactccg tcggcgaagc tattctcgat
      601 gtcctcgatc gaggtcgacg cgccgtagat gccgccccac gcgaaggtct tggccagatc
      661 gaactcggtc acgagctcgt gcgccggcat ctgatagagg cgcaccttgc cctcgatgtg
      721 aatctgcccg aggcccgcca acatggcgcg cgcgaaggcg ttgccccgct ggtagcgcga
      781 cagctgcaga tccagctcga aattccgcgc gtcgccgggg agctgatgag cgagcttgcg
      841 gttgtcgatc cgcagcttga tcttctgcgc gagtcgggtc tgctcctccg gcaggacctg
      901 tgcattgccc gcggcggtca cgctgacctc cacggcgtcg gcggcggcca ggcgtaccac
      961 ggtggaggtg tgggagaact cgggcctggg cgccgtgccg gcgcaacccg cggccgtcag
     1021 ggcggcaaca acgacgcacg agtgcagggc ccggcgcgtg cagcgggcaa ggagcctggt
     1081 ggaatgcatg tcggcctccc ggtgtcgcgg acacgcccga tgcgtgtcct tggaacacag
     1141 gagccgccgc ggcggcatcc gggtactgcc gccgcaccga actgcgcggt cgcgcgtcgt
     1201 gccggtcggc cccggctgtc agctcgaagc ggccgcaggc tcactcgccg gcgaggcagc
     1261 tctcgtggcg cgcgatgcgc gtgccagcgc gatgccggtg aggacgatga ccccgcccgc
     1321 gagctgcacc ggacgcaacc gctccccgag cagcagccag gcgtacaccg ccgcggcgat
     1381 cacctgcagg tacaggccga gcgagccgaa ggtcggacgc aggtgcgcca gcgcgtacgc
     1441 gatcaggctc tgcccgagcg cctgggcgga cagcgccagc gcaacgagca gcagccagcc
     1501 gcgcggcgtg tccggcagaa acttctcccc caacagaaac ggcaccggca gcagcagcac
     1561 gctgaagatg agcgtgctcg cgaacatgac ggtgccggtc ccatgtcggg cgcgcagccg
     1621 cgccacggcg agaatgtagg cggcgtacag ccaggcggcg ccgagcgcga ggccgtcgcc
     1681 gccgagcgcg tggccgccct gcgtgagctt cggactcacg atgagcagca gcccgccgaa
     1741 ggcgagcagc gtcgccagca gaaagccgag ccgcgggcgc tcgccccaca ccacccagac
     1801 gagcagcgtg acgacgatcg gtgcggtgtt cgcctcgaga gtggcggccc cgaccgaagt
     1861 gcgcgagagc gacaggtggt agagcgcaat gtcgccggca aagaacactc ccgcccacag
     1921 gaacgctgca tcgaggagcg agccgcgcgc gccggcgcgc gcggccggtg gcgcgcgctt
     1981 ctcgagccag gcccacagcg cgagcggcgg gagcgcgagc gcgccgcgcc agaaggcgct
     2041 cgccacggcc ccggtctccg cgacgcgcac gaagatgccg gagctgccga tcgcgagcgc
     2101 gccggcgaag agagcgagca gtgcggtgag ctccgggcgt gcggactttg tcattgttcg
     2161 gtggccggat catagtcggt cggccgcgga gcgtctgg
//
LOCUS       DATLKL010000773         1669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_65825, whole genome shotgun sequence.
ACCESSION   DATLKL010000773 DATLKL010000000
VERSION     DATLKL010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65825"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VM713_10505"
     CDS             complement(<1..741)
                     /locus_tag="VM713_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVH84734.1"
                     /translation="MDYASSLRGLLPGGRRTPGLKELLATVGGYLAPEQVGRIREAAE
                     FGASAHKGQKRLSGEPYIAHPVAAAAILADLHLDADTIVAAILHDVIEDTPTPKDQLV
                     ARFGADVAELVDGVTKLDQIKFKSREEAQAESFRKMLLAMVRDLRVILVKLADRTHNM
                     RTIEAMAPARRRAIARETLDIYAPIAERLGLYNLKLELEDLEFKALYPRRYQVLERAL
                     KRARGNQKEFLKKIDQQLNAALMKAEITA"
     gene            complement(758..1036)
                     /gene="rpoZ"
                     /locus_tag="VM713_10510"
     CDS             complement(758..1036)
                     /gene="rpoZ"
                     /locus_tag="VM713_10510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HVH84735.1"
                     /translation="MARITVEDCLHNVDNLFELVLLAAQRARRLANGAEATIPLENDK
                     PTVVALREIAAGNITAEMLREPEPAPEPAVPDADNQSSFRAPQFGLGD"
     gene            complement(1165..>1669)
                     /gene="gmk"
                     /locus_tag="VM713_10515"
     CDS             complement(1165..>1669)
                     /gene="gmk"
                     /locus_tag="VM713_10515"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HVH84736.1"
                     /translation="RTRRPTEVEGREYHFVSKPQFQELVQRGEFLEHARVFDNNYGTS
                     RAFVESQLAAGHDVLLEIDWQGAQQVRRALPECVSIFVLPPARQALAERLARRATDTA
                     EVIRRRLQDAAGDMSHSIEFDYVVVNDDFDKAVADLRRIIAGQGEDLRSDRPELKALL
                     GELLAPA"
BASE COUNT          228 a          559 c          592 g          290 t
ORIGIN      
        1 cgcggttatc tccgccttca tgagcgcggc gttcagctgc tggtcgatct tcttgaggaa
       61 ctccttctgg ttgccgcgcg cgcgcttgag cgcgcgctcg agcacctggt agcggcgcgg
      121 gtagagcgcc ttgaactcca gatcctcgag ctcgagcttg aggttgtaca gcccgagccg
      181 ctcggcgatc ggcgcgtaga tgtcgagcgt ctcgcgggcg atcgcccgcc gccgcgccgg
      241 cgccatggcc tcgatggtgc gcatgttgtg ggtgcggtcg gcgagcttca ccaggatgac
      301 gcgcagatcg cgcaccatcg cgagcagcat cttgcggaac gactccgcct gcgcctcctc
      361 gcggctcttg aacttgatct ggtcgagctt ggtgacgccg tcgacgagct cggccacgtc
      421 ggcgccgaag cgcgccacca gctgatcctt cggcgtcggc gtgtcctcga tgacgtcatg
      481 gaggatcgcg gcgacgatcg tgtcggcatc gagatgcagg tcggcgagga tggcggcggc
      541 cgccaccggg tgggcgatat agggctcgcc ggagaggcgc ttctgtccct tgtgcgccga
      601 ggcgccgaat tcggccgcct cgcggatccg tccgacctgc tcgggggcga ggtagccgcc
      661 gacggtcgcc agcaactcct tcagtcccgg ggtgcgtctt ccgccgggca gcagtccgcg
      721 gagcgaagac gcatagtcca taggtcgcgc gctgtcgtca gtctccgagg ccgaactgcg
      781 gcgcgcggaa agaggactga ttgtccgcgt ccggcaccgc gggttcgggc gccggctcgg
      841 gctcgcgcag catctccgcg gtgatgttgc cggcggcgat ctcgcgcagc gcgaccaccg
      901 tcggcttgtc gttctcaagc gggatggtcg cttccgcgcc gttggcgagc cggcgggcgc
      961 gttgcgccgc gagcagcacc agctcgaaca ggttatcgac gttgtgcaga cagtcttcta
     1021 cggtgattcg ggccatgggt cgcttacgct gagtgagggc accacacacg gggtgcgcag
     1081 ggcccttaac tataagcgaa aagtgcctgt tcgggccagt cgcgggggtt gcgggctgcg
     1141 ctgcccggcg ctgcctgggt ccggtcaagc cggcgccagc agctccccga gcagcgcctt
     1201 gagctccggg cggtcgcttc taagatcctc gccctggccc gcgatgatgc gcctgaggtc
     1261 ggcgaccgcc ttgtcgaagt cgtcgttgac gaccacgtag tcgaactcga tggagtggga
     1321 catgtcgccg gctgcgtcct gcagccgccg gcggatgacc tcggccgtat cggtcgcgcg
     1381 ccgcgccagc cgctctgcca gcgcctgccg cgccggcggc aggacgaaga tgctgacgca
     1441 ctcgggcagc gcgcggcgca cctgctgcgc gccctgccag tcgatctcga gcagtacgtc
     1501 gtggcctgcc gcgagctgcg actcgacgaa ggcgcgcgac gtaccgtagt tgttgtcgaa
     1561 cacccgcgcg tgctcgagga actcgccgcg ctgcaccagc tcctggaact gcggcttcga
     1621 gacgaagtga tactcgcgcc cctccacctc ggtcggacgg cgcgtgcgg
//
LOCUS       DATLKL010000774         2055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_201069, whole genome shotgun sequence.
ACCESSION   DATLKL010000774 DATLKL010000000
VERSION     DATLKL010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201069"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2055)
                     /locus_tag="VM713_10520"
     CDS             complement(<1..>2055)
                     /locus_tag="VM713_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVH84737.1"
                     /translation="VGVYLDESPISVALFTPDLELFDLDRFEVLRGPQGTLFGAGSEA
                     GTVRYITAQPRLGKTEIFTEASIEDVTHGDEGGFLRAAMNVPLGDNVAARLVAYEHHI
                     AGFIDAISPYGPAGADKPAGFTVNRAINSGDRSGARFALLIKPSSELSITPRIVFQKL
                     ETNGYPRVDIYNILANPYTTTQPAVTMGDRTQYRQQGDGLTDDFTLADVKVDYDAGPA
                     TLTSVTSYTHRNVQVLRDATQLTGSVTFDVFGDASVPPDFAGVRTSSPLYDRTHLNVF
                     SQELRLTSNGKQTIDWLVGGFFQHVGRHYGQDLPTQGYDALNAQFGFPLGPNPVGLYD
                     TPFYSNLFYTLKQYAAFGEATWHATDQLSVTAGLRYYKYSEDRVLNFNGVFAADTPPG
                     GVPGSVDSNGTSPRLILSYKPVDGVDLYAQASRGFRLGGINDPINLPLCSAQDKIIFG
                     GYGTWKDEKLWDYEVGMKSQWLDRRVTFNLAAFYEKIDNLQATATAGTCSSRLVIDVP
                     TSRSEGVEAELFAHPNVNWDFGISATVLDAKLTSSVISSLGVVGGLQDGNRLPTSPKT
                     QAAGSVGFTLPSAAGSADFFANFTVQYVGSSYSQFENEEPGWGQIGGAGDPNGARLIT
                     YGGVPAGTVITFNPLLPSYTLANLRFGFKTDRWQAALYVNNIADKSAELALDYERGRS
                     ARV"
BASE COUNT          361 a          627 c          716 g          351 t
ORIGIN      
        1 gacccgggcg ctgcgcccgc gctcgtagtc gagcgccagc tccgcgctct tgtcggcgat
       61 gttattgacg tagagcgccg cctgccagcg atcggttttg aagccgaagc gcaggttcgc
      121 gagcgtatag ctcggcagca ggggattgaa ggtgatcacg gtccccgcgg gcacgccgcc
      181 gtaggtgatc agccgcgccc cattgggatc gccggcgccc ccgatctggc cccaacccgg
      241 ctcctcgttc tcgaactgcg agtaggagga gccgacgtac tggacggtga agttggcgaa
      301 gaagtccgca gaccccgcag ctgagggcag cgtgaagccg acgctgcctg cggcctgcgt
      361 cttcggcgaa gtcgggaggc ggttgccgtc ctgcaggccg ccgacgacgc cgagtgagga
      421 gatgaccgac gaagtcagct tggcgtccag aactgtcgcc gagatgccga agtcccagtt
      481 gacgttcgga tgcgcaaaca actcggcctc cacgccctca ctgcgcgaag tcggcacatc
      541 gatgacgagg cgcgaggagc aggtgccggc ggtagcggtg gcctgcaggt tgtcgatctt
      601 ctcgtagaat gcggccagat tgaaggtaac gcggcggtcc agccactgcg acttcatgcc
      661 gacctcgtag tcccagagct tctcgtcctt ccacgtgccg tagccaccga agattatctt
      721 gtcctgcgcg gagcacagcg gcaggttgat ggggtcgttg ataccgccca ggcggaaacc
      781 gcgcgaggcc tgagcgtaga ggtcgacacc gtccaccggc ttgtaggaaa ggatcaggcg
      841 cggcgaggtg ccgttcgaat cgaccgatcc cggcacgccg cccgggggcg tgtcggcggc
      901 gaacacgccg ttgaagttga gcacccgatc ctcgctgtac ttgtagtagc gcaggcctgc
      961 cgtgaccgat agctggtcgg tcgcatgcca ggtcgcctcg ccgaaggccg cgtattgctt
     1021 caaggtgtag aagagattcg agtagaacgg cgtgtcgtac agccccaccg ggttcggccc
     1081 gagcgggaaa ccaaactggg cattcagggc gtcatagccc tgggtcggca gatcctgtcc
     1141 gtagtgccgt ccgacgtgct ggaagaagcc gccgaccagc cagtcgatcg tctgcttgcc
     1201 gttagacgtg agccgcaact cctgcgagaa gacattgagg tgcgtccggt catagagggg
     1261 cgagctggtg cgcactccgg cgaagtcggg cggcaccgag gcatcaccga acacatcgaa
     1321 ggtcacgctg ccggtgagct gcgtcgcatc gcgcagcacc tgcacgttgc ggtgcgtgta
     1381 ggaggtgacc gaagtgagcg tcgccgggcc agcgtcgtag tcgaccttga cgtccgcgag
     1441 cgtgaagtcg tcggtcaggc cgtccccctg ctgccggtac tgcgtgcgat cgcccatggt
     1501 cacggccggc tgggtggtcg tgtaggggtt cgcgaggatg ttgtagatat cgacgcgcgg
     1561 atagccgttg gtctcgagtt tctggaacac gatgcgcggc gtgatcgaca gctcgctgct
     1621 cggcttgatg agcagtgcaa agcgcgcgcc gctgcgatcg ccggagttga tggcccggtt
     1681 gacggtgaaa cctgcgggct tgtcggcgcc cgccggcccg tagggcgaga tcgcgtcgat
     1741 gaacccggcg atatgatgct cgtaggcgac caggcgggct gccacgttat cgccaagcgg
     1801 cacgttcatc gcggcgcgca ggaacccacc ctcatcgccg tgcgtgacgt cctcgatcga
     1861 ggcttccgtg aagatctcgg tcttgccgag gcgcggctga gcggtgatgt agcgcaccgt
     1921 gccggcttcg gatcccgcgc cgaacagcgt gccctgcgga ccgcgcagca cctcgaagcg
     1981 atcgagatcg aaaagctcga ggtcgggagt gaagagggcg accgagatcg gcgactcatc
     2041 gagatagacg ccgac
//
LOCUS       DATLKL010000775         4404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_178538, whole genome shotgun sequence.
ACCESSION   DATLKL010000775 DATLKL010000000
VERSION     DATLKL010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178538"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..759
                     /locus_tag="VM713_10525"
     CDS             205..759
                     /locus_tag="VM713_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HVH84738.1"
                     /translation="MVRLALAPDEPALGSASAPLTMVEFTDYQCPYCRRFQAEVWPKL
                     KARYIDTGKLRYIARDLPLEFHAMAAPAAEAAHCAGEQGKFWEMHAALLGGAADLGAG
                     GIGQRARALGLDTSRFGACLEHKRYAALVVAHEREADAAGIRGTPGFVVGRAAHGELT
                     GVRVQGALPYEEFAELLRELLAGG"
     gene            complement(776..1879)
                     /locus_tag="VM713_10530"
     CDS             complement(776..1879)
                     /locus_tag="VM713_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HVH84739.1"
                     /translation="MSGRLWQIEFLGNTLGAWTIALAIFLVTFTALPLAKRLIAAQRR
                     RLSVRAPLKVQIDLAALLAGRTHRLFLWGAALWLAARGLAFPPQLERWLTVGFVLLLW
                     MQIALWAMGAVRFAIDLRRQRSTGPDTLLKSSMEVILFATGLVIWGVAALLALDSLGV
                     EIRPLLAGLGIGGIALALAVQTVLADLLASLSIALDKPFGLGDFLTVDDCQGTVEHIG
                     VKSTRLRSINGEQIIISNGDLLKARVRNYGRLTERRALIVLPVHYETPVATLAAVPRV
                     VREIIEATPDVRFDRCHLLRTTEAALEFEAVFFVTRPEYRAYADAQQSINLRILERFR
                     TMEVSFIATAPRPVRLHPPLAPAADASGQQRLL"
     gene            complement(1925..3343)
                     /gene="ntrC"
                     /locus_tag="VM713_10535"
     CDS             complement(1925..3343)
                     /gene="ntrC"
                     /locus_tag="VM713_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HVH84740.1"
                     /translation="MNSDSLRVWLVDDDASIRWVLERALKHGGMTARVFDAAEPALEA
                     LRREAPDVLITDIRMPGASGLELLKRIRDARPALPVIVMTAHSDLGSAVSAYEGGAFE
                     YLPKPFDIDQAVALARRAANAGMQGGAEAGGTPRIPALLGRAPAMQQVFRAIGRLARS
                     SMTVLVTGESGTGKELVARALHDHSPRAGKPFIALNTSAIPADLLESELFGHERGAFT
                     GADALRRGRFEQADGGTLFLDEIGDMSMPLQTRLLRVLAEGEFYRVGGQTAVRVDVRV
                     IAATHQDLRERVTRGLFREDLYHRLNVIRIELPPLRTRAGDIPDLLAHYLEAAAHELG
                     VEPKTLAADAHALLSAYDWPGNVRELVNLCRRLSVLAPGREVRLEDLPAELVSAAGAG
                     PKDADWAAQLASWAERATLSATQPLLDAAQPQFERVLIRAALRRTHGHRQEAARLLGW
                     GRNTLTRKLKELGMSGPEGAGD"
     gene            complement(3340..>4404)
                     /gene="glnL"
                     /locus_tag="VM713_10540"
     CDS             complement(3340..>4404)
                     /gene="glnL"
                     /locus_tag="VM713_10540"
                     /inference="COORDINATES: protein motif:HMM:NF008293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HVH84741.1"
                     /translation="HFDPAELLDALSTGILMLDAQLCPIYANVAAQDLLAFSLNMSRG
                     RPFGDFLHEAGALNVILKRALETGECLADRELTLKPASSPREPRTLDVTVTPLAGLTG
                     THLLIELADTTQRQRISRDNDLLARLDGSRLMIRQLAHEIKNPLGGLRGAAQLLEREL
                     PAPSLREYTQVIIGEADRLTALVESIAGPSRPPSKTLMNVHEICEHVYHLLRAEARTG
                     ILVERDYDPSLPNASLDRHQLIQALLNVGRNALQALESHGDAGHVVLRTRARSNVSIG
                     STRHRLVAAVQVEDSGPGVPEQLRASIFYPLVTGRANGTGLGLAVAQELVTRNGGIIE
                     FESEPRRTVFTLLLPLGDET"
BASE COUNT          656 a         1505 c         1625 g          618 t
ORIGIN      
        1 tgaccgtcgc gaagccggtc ggcgaggcct accatccgca gcgccccatg cagcgcgaag
       61 agaaacggga cgagaaacgc gaggaccggc cgaaggaaga aaggcattaa cgcctctacc
      121 acggcggcgc ggctcgcggt cgccgcggtt ttcgccgcgg ctgcgctcgt tggcgatgcc
      181 gcctccgccg gcgaggatgc agatgtggtg cgcctcgcgc tggccccgga cgaacccgcg
      241 cttgggagcg cgagcgcgcc gctcaccatg gtggagttca ccgactacca gtgcccctac
      301 tgccggcgtt ttcaggccga ggtgtggcca aagctcaagg cccggtacat cgataccggg
      361 aagctgcggt acatcgcgcg cgacctgccg ctcgagtttc atgccatggc ggcgccggcc
      421 gccgaggccg cgcactgtgc cggcgagcag ggcaagttct gggagatgca tgcagcgctg
      481 ctcggcggtg cggcggacct cggagccggc gggattgggc agcgggcgcg cgccctcggc
      541 ctcgacacga gccgcttcgg cgcgtgcctc gagcacaaga ggtacgccgc gttggtcgtg
      601 gcgcacgagc gggaagccga cgccgccggc atccgtggca cacccggctt cgtcgtcggc
      661 cgcgccgcgc acggcgagct caccggcgtg cgcgtacagg gggcgctgcc ttacgaggaa
      721 ttcgccgagc tgctgcgcga gctgctcgcg ggcggttgag aagtggttac agcgcttaca
      781 gcagccgctg ctgcccgctt gcatccgcgg ccggcgcgag cggcgggtgc agccgcacgg
      841 gacgcggcgc ggtggcgatg aagctcacct ccatcgtgcg gaagcgctcg agaatcctca
      901 agttgatgct ctgctgcgca tccgcgtagg cccgatactc gggccgcgtg acgaagaaca
      961 cggcctcgaa ctcgagcgcc gcctccgtgg tgcgcagcag gtggcagcgg tcgaagcgca
     1021 catcgggtgt cgcctcgatg atctcgcgca ccacgcgcgg caccgcggcg agcgtcgcca
     1081 ccggcgtctc gtagtgcacc ggcagcacga tgagcgcgcg ccgctcggtg agacgcccgt
     1141 agttgcgcac gcgcgccttc agcaggtcgc cgttcgagat gatgatctgc tcgccattga
     1201 tgctgcgaag gcgcgtgctc ttcacgccga tgtgctcgac cgtcccctgg caatcatcga
     1261 cggtgaggaa gtcgccgagc ccgaagggct tatcgagcgc gatcgagagc gaggcgagga
     1321 gatcggcgag caccgtctgc actgcgagcg cgagcgcaat gccgccgatg cccaggcccg
     1381 cgagcagcgg ccggatctcg acccccaagc tgtcgagcgc gagcagcgcc gcgacccccc
     1441 agatgacgag ccccgtggcg aacaggatca cctccatcga gctcttgaga agcgtgtcgg
     1501 gtccggtgga gcgctggcgg cgcaggtcga tcgcgaagcg caccgccccc atggcccaca
     1561 gcgcgatctg catccacagc agcagcacga atcccacggt cagccagcgc tcgagctgcg
     1621 gcgggaaggc gagaccgcgc gcggcgagcc acagcgccgc gccccacagg aacagccgat
     1681 gggtgcgccc ggcgagcagc gccgcgagat cgatctggac tttgagcggc gcgcgcaccg
     1741 agagccgccg gcgctgcgcg gcgatcagac gtttggcgag cggcagcgcc gtgaaggtga
     1801 cgaggaagat agcgagcgcg atcgtccagg cgccgagcgt gttgcccagg aactcgatct
     1861 gccacaagcg gcccgacatc ggcttggccc cttccgcgtt gctcgcttct taagggcgcg
     1921 gcggctagtc cccggcgcct tcgggcccgc tcatgccgag ctccttcagt ttccgtgtca
     1981 gggtgttgcg tccccagccg aggagcctcg ccgcctcctg gcgatgtccg tgcgtgcggc
     2041 gcagcgccgc ccggatcagc acgcgctcga actgcggctg cgctgcatcg agcagcggct
     2101 gtgtcgcgct caaagtggcg cgctcggccc agctggcgag ctgcgcggcc cagtccgcat
     2161 ccttcggtcc cgcgccggcg gcgctcacga gctccgccgg caggtcctcg aggcgcacct
     2221 cgcgcccggg agcgagcacc gacagccgcc ggcagagatt cacgagctcg cgcacgttcc
     2281 ccggccagtc gtaggcgctg aggagcgcgt gcgcatcggc ggcgagcgtc ttcggctcga
     2341 cgccgagctc gtgggcggcg gcctcgaggt aatgggcgag gaggtcgggg atgtcgccgg
     2401 cacgcgtgcg cagcggcggc agctcgatgc ggatgacgtt gaggcggtgg tacagatcct
     2461 cgcggaacag gccgcgcgtg acgcgctcgc gcaggtcctg gtgcgtcgcg gcgatgacgc
     2521 gcacgtcgac gcgcaccgcg gtctgcccgc cgacgcgata gaactcgccc tcggcgagca
     2581 cgcgcaacag gcgcgtctgc agcggcatcg acatgtcgcc gatctcgtcg aggaacagcg
     2641 tcccgccgtc ggcctgctcg aagcgcccgc gccgcagcgc atcggcgccg gtgaaggcgc
     2701 cgcgctcgtg gccgaagagc tcggactcga ggaggtcggc ggggatcgcc gaggtgttga
     2761 gcgcgatgaa gggcttgccg gcgcgcgggc tgtggtcgtg cagcgcgcgc gccacgagct
     2821 ccttgccggt gccggactcg ccggtcacca gcacggtcat cgaggagcgc gccaggcggc
     2881 cgatggcgcg gaacacctgc tgcatcgccg gcgcgcgccc gaggagcgcg gggatgcgcg
     2941 gcgtgccgcc cgcctcggcg ccgccctgca tgccggcgtt ggcggcgcgc cgtgcgagcg
     3001 ccacggcctg gtcgatgtcg aagggtttcg gcaggtactc gaacgccccg ccctcgtagg
     3061 ccgacacggc gctccccaag tcagagtgcg cggtcatcac gatgacgggc agcgccggcc
     3121 gcgcgtcgcg gatgcgcttg aggagctcga gccccgaggc gcccggcatg cggatgtcgg
     3181 taatcagcac gtcgggagcc tcgcgccgca gcgcctccag cgccggctcc gccgcatcga
     3241 acacgcgcgc ggtcatgccg ccgtgcttga gcgcgcgctc gagcacccag cggatcgagg
     3301 cgtcgtcgtc gaccagccac acccgcagtg agtcgctgtt catgtttcat ctccgagcgg
     3361 caggagcagt gtgaacacgg tgcgccgcgg ctcgctctcg aactcgatga tgccgccgtt
     3421 acgcgtcacg agctcctggg ccaccgcgag cccgaggccc gtgccgttgg cgcgcccggt
     3481 gacgagcgga tagaagatcg aggcgcgcag ctgctcgggg acgccgggac ctgagtcctc
     3541 gacctgcacg gcggcaacca ggcgatggcg cgtcgagccg atgctgacgt tgctgcgcgc
     3601 gcgggtacgc agcaccacat ggccggcgtc gccgtgcgac tcgagcgcct gcagcgcgtt
     3661 gcggccgacg ttgagcagcg cctggatcag ctgatggcgg tcgagcgagg cgttcggcag
     3721 gctcggatcg tagtcgcgct ccaccaggat tccggtgcgc gcctcggcgc gcaacaggtg
     3781 atagacgtgc tcgcagatct cgtgcacgtt catcagcgtc ttgctgggcg ggcggctggg
     3841 acctgcgatc gactcgacca gcgcggtgag gcgatcggct tcaccgatga tgacctgcgt
     3901 gtactcgcgt aaggaaggcg ccggcagctc gcgctcgagc agctgcgccg cgccgcgcag
     3961 gccccccagg gggtttttga tctcgtgggc gagctgccgg atcatgaggc ggctgccgtc
     4021 gaggcgcgcg agcaggtcgt tgtcgcgcga gatgcgctgg cgctgcgtgg tgtcggcgag
     4081 ctcgatcagc aggtgcgtgc cggtgagccc cgcgagcggc gtcaccgtga catcgagggt
     4141 gcgcggctcg cgcggcgagc tggcgggctt gagcgtcagc tcgcggtcgg cgagacattc
     4201 gccggtctcg agggcgcgct tgaggatgac gttgagcgcg cccgcctcgt gaagaaaatc
     4261 gccgaacggg cggccgcggc tcatgttgag actgaacgcg agcaggtcct gcgccgccac
     4321 gttggcgtag atcgggcaga gctgcgcatc gagcatcagg atcccggtcg agagcgcatc
     4381 gagcagctcc gccggatcga agtg
//
LOCUS       DATLKL010000776         1925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_88326, whole genome shotgun sequence.
ACCESSION   DATLKL010000776 DATLKL010000000
VERSION     DATLKL010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88326"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VM713_10545"
     CDS             <1..1127
                     /locus_tag="VM713_10545"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HVH84742.1"
                     /translation="AAFRRALAVKPGFPTALFHLAHLRGAEPTDAEVGAIERALEAPR
                     LPRQARIGLSFALARLCDERGEYDKAFLHCKRANDMKAADQAFDHEGLARHVDALVAT
                     FDRGFFAARRGLGSPSERPVFVVGMPRSGTTLVEQILASHPEVRGAGELAASNAMVEG
                     LARLPNATAAGKAYPEAAGLLDQEEAQALAERYLEAVGRDAREGEGGEPRRVTDKMTG
                     NFMRLGLIALLLPRARVIHCQRDPFDTCLSCYFQNFLEPFPFTSELSRLGKYYREYER
                     LMAHWRAVLPAPMLEVPYEALVADPEPWCRRMLEFCGLPWDERVLRFFATERAVQTAS
                     FWQVRQPIYLSSVGRWRHYRAHLGSLFDALGREPDAHAAD"
     gene            1406..>1925
                     /locus_tag="VM713_10550"
     CDS             1406..>1925
                     /locus_tag="VM713_10550"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HVH84743.1"
                     /translation="MEEPATLSYPYPIEKGLDFPDYRAVAERNAGIYVRVRIVNTGLA
                     DPGMERGRGGPSDVLRYDSGFIADPRGYVIPAAHVTVSTKYRAEVITLDGRRYSGRVV
                     AIEQQRDLALIKFVPFAGMTAARFADSAQLTPGEPALAIGMLAPRQHLAGVVSVGKVM
                     GWPRARGVHYRDS"
BASE COUNT          273 a          689 c          653 g          310 t
ORIGIN      
        1 ccgccgcctt ccgccgggcc cttgcggtca aaccgggctt cccgacggcc ctgttccacc
       61 ttgcccatct gcggggcgct gagccgaccg acgccgaggt cggcgcgatc gaacgggcgc
      121 tcgaggcccc caggctgccg cgtcaggcgc gcatcgggct cagcttcgcg ctcgcgcggc
      181 tgtgcgacga gcggggcgaa tacgacaagg cattcctgca ctgcaagcgg gcgaacgaca
      241 tgaaggcggc ggaccaggcc ttcgatcatg agggcttggc gcgccacgtg gacgccctcg
      301 tcgccacctt cgaccgcggc ttcttcgcgg cgcggcgggg gctcggctcg ccctcggagc
      361 ggcccgtctt cgtggtcggc atgccgcgct cgggcaccac gctggtcgag cagatcctgg
      421 cgagccaccc cgaggtgcgc ggtgcgggcg agctcgcggc gagcaacgcg atggtcgaag
      481 ggctggcgcg gctgccgaat gcaacggctg cgggcaaggc ctatcccgag gcggcggggc
      541 tcctcgatca ggaggaagcc caggcgctcg ccgagcgcta tctcgaagcc gtcggccgag
      601 acgcccgcga aggcgagggc ggcgagccgc gccgcgtcac cgacaagatg accggcaatt
      661 tcatgcgcct cggcctcatc gccctgttgc tgccgcgcgc ccgggtgatc cactgccagc
      721 gcgacccctt cgacacctgc ctctcctgct atttccagaa cttcttggag ccattcccgt
      781 tcacgagcga gctttcgcgc ctcggcaaat attaccgcga gtacgagcgg ttgatggccc
      841 actggcgcgc ggtgttgccg gcacccatgc tcgaggtccc ctacgaggcc ctggtcgccg
      901 accctgagcc ctggtgccgg cgcatgctgg agttttgcgg attgccatgg gacgaacggg
      961 tgctgcgctt cttcgccacc gagcgcgcgg tgcagaccgc gagcttctgg caggtgcgcc
     1021 agccgatcta tctgagctcg gtcgggcgct ggcggcacta ccgcgcccat ctcggctcgc
     1081 tgttcgatgc gctcggacgc gagcccgatg cgcatgccgc cgattgatta aagtgacgat
     1141 tctaaccgtc cgtacccttt cgtcatgccg gcgaaagcag cgggacgctg cgctaagctt
     1201 gcgtcccgcc ccgattctgg attccggctc gccgcttcgc tctggccgga atggcgctgc
     1261 agcaaacggg ttcgatcacc aaagacacga ctttggcgcc tcggttggcc catcccatgc
     1321 cgcgatgccg cgggcagcgg ctctcctcct tctcctcggt ctcgtggcct gttccggacc
     1381 gcacaaacct cccgagggcg cgcgcgtgga ggagcccgcc accctcagct atccctaccc
     1441 gatcgagaag gggctcgact tccccgatta ccgcgccgtc gccgagcgca acgccggcat
     1501 ctatgtccgc gtgcgcatcg tgaacacggg gctcgcggat cccgggatgg agcggggccg
     1561 cggcggcccg agcgacgtgc tccgctacga cagcggcttc atcgccgatc cgcgcggcta
     1621 cgtgatcccg gccgcgcacg tcacggtctc gaccaagtac agggccgagg tgatcacgct
     1681 cgacgggcgg cgctacagcg gccgcgtcgt ggccatcgag cagcaacgcg atctcgccct
     1741 gatcaagttt gtccccttcg ccggcatgac ggcggcgcgc tttgccgaca gcgcgcaact
     1801 gacgccgggg gagcccgcgc tcgccatcgg catgctggcg ccgcgacagc acttggccgg
     1861 cgtggtgagt gtcgggaagg tgatgggctg gccgcgcgcg cggggcgtgc actaccggga
     1921 ttccc
//
LOCUS       DATLKL010000777         1638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_223229, whole genome shotgun sequence.
ACCESSION   DATLKL010000777 DATLKL010000000
VERSION     DATLKL010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223229"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..116)
                     /locus_tag="VM713_10555"
     tRNA            complement(41..116)
                     /locus_tag="VM713_10555"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(81..83),aa:His,seq:gtg)
     gene            complement(138..214)
                     /locus_tag="VM713_10560"
     tRNA            complement(138..214)
                     /locus_tag="VM713_10560"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Arg,seq:tct)
     gene            357..1454
                     /locus_tag="VM713_10565"
     CDS             357..1454
                     /locus_tag="VM713_10565"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HVH84744.1"
                     /translation="MSKLVLSAREPAVDRGPDGARTAIATDAPLPPARVDGSLAVGEA
                     ALAEVRRLLGAWLAHEPGARLGSDPEELHQLRVTARRIDATLSLFKQHLPPAVVKARK
                     RAKALLRSLGATRDLDVQLAGLNTYCGEVPEEERVALEPLKERLTIERARARARMIRA
                     LDSEPTRRWIQTLTAATEAPPAANDAGAATAAAVMPQRVRRRFRRLRKAVRRLRADSS
                     MDDYHKVRRRAKQLRYALESGLSLYGKPADEMLKALRRMQDKLGASQDAHMARSHLVT
                     LAADPACALPPATLFHMGKLAEHHARITSEARSSVTKSWRKVRGRRWKALRARLGEFE
                     PGVTESPVPVPGPVAALANVSPAAETARLSH"
BASE COUNT          249 a          599 c          552 g          238 t
ORIGIN      
        1 aaaaggcgtg gcttttgcct gctgcgcatc acggggcaag tggggtggac gatgggactc
       61 gaacccacga caaccgggat cacaacccgg ggctctacca actgagctac gtccaccgtc
      121 cgtacccccg gcgccggtgg cgtgcccggc aggactcgaa cctgcgaccc tcggcttaga
      181 aggccgatgc tctatccgcc tgagctacgg gcacgactgc aaggcgcccc tagccggccc
      241 cgccgcacgg ggggcgcgca tcatacgtaa gtggcttccg gagcgtcaat tttccttggc
      301 ctgctgcgcc gcggcctttg ttacactcgc tgtatcccga tgaggtgccc tgctccttga
      361 gcaagctcgt cttaagcgcc cgtgagcccg ccgtcgatcg cggcccggac ggagcgagga
      421 cggcgattgc gaccgacgcc cctctgccgc ccgccagagt ggatggctcg ctcgcggtcg
      481 gtgaggcagc gctcgccgag gtgcgcagac tcctcggtgc ctggctcgcg catgagccgg
      541 gtgcgcgcct tgggtctgac cccgaggagc tgcaccagct gcgcgtcacc gcgcgacgca
      601 tcgatgcgac cttgagcctc ttcaagcagc acctgccacc ggccgtggtc aaggcacgca
      661 agcgggcgaa ggccctgttg cgctcactcg gcgccacgcg cgatctcgac gtgcaacttg
      721 cgggcctcaa cacctactgc ggggaggttc ccgaagagga gcgggtagcg cttgagcccc
      781 tgaaggagcg cctcacgatc gagcgggctc gcgcccgcgc gcgcatgatc cgtgcgctcg
      841 actccgagcc gacacgccgc tggatccaga cgctcacggc tgcgacggaa gcaccgcccg
      901 cggccaacga cgcgggagcc gccaccgccg ccgcggtcat gccgcagcgg gtacgccggc
      961 gctttcgccg gctgcgcaag gcggtgcggc ggctgcgcgc cgattcctcg atggacgact
     1021 atcacaaggt gcgccgccgc gcgaagcagt tgcgctacgc gctcgaatcc ggcctgagtc
     1081 tctacggtaa acctgccgac gagatgctga aggcgctgcg ccggatgcag gacaagctcg
     1141 gtgcgtccca ggacgcgcac atggcccgca gtcacctcgt gacactcgcg gccgacccgg
     1201 cctgcgctct gccgccggcg acgctctttc acatggggaa gctcgccgag caccacgcgc
     1261 gcatcacgag cgaagcacgc tcgagcgtga ccaagtcgtg gcgcaaggtg cgcggtcggc
     1321 gctggaaggc gctgcgcgcc aggctagggg agttcgagcc gggcgtgact gaatcacccg
     1381 ttccggtgcc ggggccggtc gcggccctcg ccaacgtctc acccgccgcg gaaaccgctc
     1441 gcctgagtca ctgatccgca ccgggcgtgc cgctcgagct cctgatcgtc cgccatgcca
     1501 tcgccctcga gcgcgatgcc aggcgctggc cggacgacgg cgaacgcccc ctctctcccc
     1561 gcggcgtcgt ccgtgctcgc aaggccgccg ccggcctgaa acagctggtg cccgcgcccg
     1621 ggcgtgtgct ggcgagcc
//
LOCUS       DATLKL010000778         2337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_43216, whole genome shotgun sequence.
ACCESSION   DATLKL010000778 DATLKL010000000
VERSION     DATLKL010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43216"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..2209
                     /locus_tag="VM713_10570"
     CDS             71..2209
                     /locus_tag="VM713_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVH84745.1"
                     /translation="MNAILNTSRRDFLKGSAAVGGGLIVGLCLPGGSPAADLKPVEAN
                     AWVRIAPDNKITLICHRNEMGQDVHTSLALLVAEELGVDVRKVAIEQAPVAPVYINSL
                     LGAQITGGSTSVRDAWLKLREAGATARVLLTTAAAQRWGVSADECVAADGFITNKSGK
                     RLSYGELADEAARLPAPKSVALKKPADFTQIGKLRHRLDTPAKARGQAVYGLDVKQPG
                     MVYASLEQCPVIGGKVAAVDDSRAKAVPGVLAVVNIGEGVAVVADHYWTAKTARDALN
                     ITWDYGPAATLTTEKIRATLKDTAAKDGAIVRQAGDAAAALKGAAKTLHAEYQLQLLA
                     HATLEPMNCLARVGDHGCDIWTSTQYPQGAQAVAAARSGVEAAKVRIWPQFIGGGFGR
                     RLDVDFIGQAAAIARAVPGKPVKLIWTREDDTRNDFYRPASYHTLSGGLDADGRLVAF
                     DYKMVSSSITNRLFPGVVKNDLDPFMMEGSVDLTYDIPNALQRVVIQEYGLRSGYWRS
                     VSLPLNAFAIEGFVDELAHAAGRDPLAFRLELLDKQPRQKAVLERVARESGWGRGLPS
                     DTALGLAMMESYETYQALVARISRSGSGLRLESLVYVVDPGIAVHPDQVVAQIQSGAV
                     SGLINTLSSKITLKDGRVEQSNFDTFPLLRMRQMPRIEVIVLEGGDKPGGMGEVGVPL
                     VAPAVANAVFALTGKRVRSLPLADSGVEFA"
BASE COUNT          412 a          758 c          789 g          378 t
ORIGIN      
        1 cgctgcggca cctacgtccg tatccgcgcc gcgatccacg cagccgccca gagcctcaag
       61 gggtgaagcc atgaacgcca ttctcaacac ctcgcggcgc gacttcctca aaggctcagc
      121 cgccgtcggt ggcggcctga tcgtcggcct ctgcctgccg ggcgggagtc ccgccgcgga
      181 cctcaagccg gtcgaggcca acgcctgggt gcggattgcg cccgacaaca agatcaccct
      241 catctgccac cgcaacgaga tgggccagga cgtgcacact tcgctcgccc tgctggtcgc
      301 cgaggagctg ggtgtcgacg tgcgcaaagt ggcgatcgag caggcgccgg tggcgccggt
      361 ctatatcaat tcgctgctcg gtgcgcagat tacgggcggc agcaccagcg tccgtgatgc
      421 gtggctcaag ctgcgcgaag cgggtgccac tgcgcgggtg ctgctgacca cggccgccgc
      481 gcagcgttgg ggggtctccg ccgacgaatg cgtagcggct gacggcttca ttaccaacaa
      541 gagcggcaag cggctgagct acggcgagct cgctgacgaa gcggccaggc tgccggcgcc
      601 aaagagtgtc gccctcaaga agcccgccga cttcacgcag atcggcaagc tgcgacatcg
      661 cctggatacg cccgcgaaag cgcgcggaca agcggtgtac ggcctcgatg tgaagcagcc
      721 gggcatggtg tacgcatccc tcgagcagtg tccggtgatc ggcggcaagg tcgccgcggt
      781 cgatgacagt agggcaaagg cggtgccggg agtgctcgcc gtggtcaaca taggtgaggg
      841 agtcgccgtg gtcgccgacc actactggac cgcaaaaacc gcgcgcgacg cgctcaacat
      901 cacctgggat tacggccccg cggcaaccct cacgaccgag aagatccgcg ccacgctgaa
      961 ggatacggcc gcgaaggatg gtgcgatcgt ccggcaggcg ggagatgccg ccgccgcact
     1021 caaaggggcg gccaagacgc tgcacgccga gtaccaactg cagctgctcg cccacgcgac
     1081 gctcgagccg atgaattgtc tcgcgcgggt cggcgaccat ggctgcgaca tctggaccag
     1141 cacgcaatac ccacagggcg cacaggccgt ggcggcggct cgcagcggcg tcgaggcggc
     1201 caaagtgcgc atctggccgc agtttatcgg tggcggattc ggccgccggc tcgacgtcga
     1261 cttcattggt caggccgcgg ccatcgcgcg cgcggtgccc ggcaagccgg taaagctcat
     1321 ctggacgcgc gaggatgaca cgcgtaacga cttctaccgt ccggcgagct atcacacgct
     1381 ctctggcgga ttggatgccg acggccgcct cgtcgcattc gactacaaga tggtctcatc
     1441 gtcgatcacc aaccgcctgt tcccgggcgt agtaaagaac gacctcgatc cgttcatgat
     1501 ggaaggctcg gtcgacctca cttacgacat accgaacgcg ctgcagcggg tggtgatcca
     1561 ggagtacggg ctgcgctccg gttactggag gtcggtgagt cttccgctca atgcctttgc
     1621 catcgagggt ttcgtcgacg agctcgcgca cgcggcgggc agggacccgc tggcttttcg
     1681 cctcgagctg ctcgacaagc agccgcgcca gaaggcggtg ctggagcgcg tcgcgcgcga
     1741 gtcaggctgg ggtcgcggcc tgccgtcgga caccgccctg gggcttgcga tgatggagag
     1801 ctacgagact tatcaggcac tggtcgcccg gatttccagg tccggatcgg ggctcaggct
     1861 ggaaagcctc gtctacgtcg tggatcccgg tatcgccgta cacccggatc aggtcgtcgc
     1921 gcagatccaa tcgggcgccg tatccgggct catcaacacg ttgagcagca agatcacgct
     1981 caaggacggg cgggtcgaac agagcaactt cgacaccttc ccgctgctgc gcatgcggca
     2041 gatgccacgc atcgaggtga tcgtcctcga gggcggcgac aagcctggcg ggatgggcga
     2101 ggtgggtgtg ccgctggtgg caccggcggt cgccaatgcg gtcttcgcgc tcaccggcaa
     2161 gcgagtgcgt tccctgccgc tcgcagactc gggcgtagag ttcgcgtagg tcagcattat
     2221 gagccgactg atcatgatcg tcgcgctcgc ggccacgtac ttcgtgttcg cgatgctgct
     2281 caacagcgtc gcgccggtca tcctgcagtc gatgctgacc ttcggcgtcg acaaggt
//
LOCUS       DATLKL010000779         2642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_223247, whole genome shotgun sequence.
ACCESSION   DATLKL010000779 DATLKL010000000
VERSION     DATLKL010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223247"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..972)
                     /locus_tag="VM713_10575"
     CDS             complement(88..972)
                     /locus_tag="VM713_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HVH84746.1"
                     /translation="MRRFIFERQPVRGHWVRLESAWRELRAHGHYPEPVERLLGEAVA
                     AVVLLAASLKFHGTLTFQLQGDGAVRLLVAQCTHDFRLRALARFDAGAVAALPADAPR
                     GALFRALVGSGGRLTVTVEADEKGGRYQGVVPLEGGSLAESLEAYFASSEQLPTRVLL
                     AADAAQGAGLLVQKLPGSGGAGEEESNAAWNDAQQGIERLKEPELLEHPVEELLARGF
                     SERDLRLFRGSPVHFECRCGEGRVTGLLRALGEEEVREVLREQGSVTVTCEFCSRPYR
                     FDRAAVDVLFASGPGEIH"
     gene            complement(1052..2491)
                     /gene="gatB"
                     /locus_tag="VM713_10580"
     CDS             complement(1052..2491)
                     /gene="gatB"
                     /locus_tag="VM713_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HVH84747.1"
                     /translation="MSEWETVIGLEIHAQLATRSKIFSGSATAYGAAPNSQANLVDLG
                     YPGVLPVLNVAAVRMAVKLGLALNARVAPTSVFARKNYFYPDLPKGYQISQYELPIVA
                     HGSLDIVLDDGHVKRVGITRAHLEEDAGKSLHEGLPGVTGVDLNRAGTPLVEIVSEPE
                     LRSAKEAVAYMKKVHTLVRYLEICDGNMQEGSFRCDANVSVRKAGATKFGTRAEIKNL
                     NSFRFVERAINYEVARQIELLEGGGTVVQETRLYDPDKGETRSMRSKEEANDYRYFPD
                     PDLLPVVLDAAFIESVRATLPELPDQKAARFVSQYGLPAYDAGVLTASRELADYYEAV
                     VRAAPADAKLAANWVMGELAAALNKESLEVTATRMTAERLAGLLKRISDQTISGKLAK
                     EVFEAMWSGGADADAVIAERGLRQITDSAAIERVIDEVMQRNPGQLAEYRAGKDKLFG
                     FFVGQVMKATGGKANPAQLNELLRRKLSG"
BASE COUNT          412 a          955 c          868 g          407 t
ORIGIN      
        1 ctagagggcc tcaagggact tcctcaacgc gggcgctcgg tgagccaatt gcctctctaa
       61 taagacaatc gcgcccgatt ccccgtctta gtggatctcc cccggtcccg acgcgaagag
      121 cacatcgacc gcggcccgat cgaagcgata agggcgtgag cagaactcgc acgtcacagt
      181 cacagacccc tgctcgcgca gtacctccct gacctcctcc tcgccgagcg cgcgcaggag
      241 acccgtcacc cggccctcgc cgcagcggca ttcgaagtgc acgggcgatc cgcgaaaaag
      301 ccgtaggtcg cgctcggaga agccgcgcgc caggagctct tccaccgggt gctcgagcaa
      361 ctccggctcc ttgagtcgct cgatgccctg ctgtgcgtca ttccaggcgg cgttcgactc
      421 ctcctcgcca gccccgccgc tgccggggag cttctgcacc aggagccccg ctccctgggc
      481 tgcatccgcc gccaggagga cccgggtcgg cagctgctcg gacgaggcga agtaggcttc
      541 cagcgactcc gccagcgagc cgccctcgag cggcacgacg ccctggtagc gccctccttt
      601 ctcatcggcc tcgacggtga cggtgagacg gccgcccgac cccaccagcg cccgaaacag
      661 cgcgccgcgc ggggcgtcgg ccggcagggc ggcgaccgcg cccgcatcga agcgcgccag
      721 cgcacgcagg cggaagtcat gggtgcattg cgcgaccagg agccgcaccg cgccgtcgcc
      781 ctgcagctgg aaggtgagcg tgccgtggaa cttgaggctc gccgccagca gcaccaccgc
      841 ggccaccgcc tcccccagca gccgttccac cggctccgga tagtggccgt gagcgcgcag
      901 ctcccgccag gcgctctcga ggcgcaccca atgcccgcgt accggttgtc tttcaaagat
      961 gaaacgccgc acgcggtcca tcggcccaca atggggcagc cgcgcggcta actcaactct
     1021 cgcacccccg ccgggacggc ggccgagggc ctcagccgct gagcttcctg cgcagcagct
     1081 cgttgagctg cgccgggttg gccttgcccc cggtcgcctt catgacctgg ccgacgaaga
     1141 agccgaagag cttgtccttg cccgcgcggt actcggcgag ctggcccgga ttccgctgca
     1201 tcacctcgtc gatcacccgc tcgatggcgg cgctgtcggt gatctgccgc aggccgcgct
     1261 ccgcgatcac cgcgtcggcg tccgcgccgc ccgaccacat ggcctcgaag acctccttgg
     1321 cgagcttgcc ggagatggtc tggtcggaaa tgcgcttgag gagccccgcg agccgctcgg
     1381 ccgtcatgcg cgtcgcggtc acctcgaggc tctccttatt caatgcggcg gcgagctcgc
     1441 ccatgaccca gttggcggcg agcttcgcgt ccgcgggggc cgcgcgcacg accgcctcgt
     1501 agtagtcggc cagctcgcgg ctcgcggtca gaaccccggc gtcgtaggcc gggagcccat
     1561 actgcgagac gaagcgcgcc gccttctggt cgggaagctc gggaagcgtc gcgcgcaccg
     1621 actcgatgaa ggccgcgtcc agcaccaccg gcagcaggtc cgggtcgggg aagtagcggt
     1681 aatcgttggc ctcctccttg gagcgcattg agcgcgtctc gcccttatcc ggatcgtaga
     1741 gacgggtctc ctgcacgacc gtcccgcccc cctcgaggag ctcgatctgg cgcgccacct
     1801 cgtagttgat ggcgcgttcg acgaagcgga aggaattgag gttcttgatc tcggcgcggg
     1861 tgccgaactt cgtggctcct gccttgcgca ccgagacatt ggcgtcgcag cggaacgagc
     1921 cctcctgcat gttgccgtcg cagatctcga ggtaacgcac cagcgtgtgg accttcttca
     1981 tgtaggccac cgcctccttg gcagagcgca gctcgggctc ggagacaatc tcgaccagcg
     2041 gcgtcccggc gcgattgaga tcgacgcccg tcacccccgg cagcccctcg tgcagcgact
     2101 tgccggcgtc ctcctcgagg tgcgcgcgcg tgatgccgac ccgcttcacg tgcccgtcgt
     2161 ccagcacgat gtcgagcgag ccgtgggcca cgatcggcag ctcgtactgg ctgatctggt
     2221 agcccttcgg cagatccgga tagaaataat tcttgcgcgc gaacaccgag gtcggagcga
     2281 cgcgcgcgtt gagcgcgagc ccgagcttga ccgccatgcg caccgccgcg acgttcagca
     2341 ccggcagcac gccggggtag ccgaggtcga cgagattcgc ctggctgttg ggggccgcgc
     2401 cgtaggcggt cgcggagccc gagaagatct tggagcgcgt cgcgagctgc gcgtggatct
     2461 cgaggccgat gacggtttcc cactcgctca tgcgtgcctc acgcatagcc tgcgggcaca
     2521 cgcgtgtgcc agtcggtggc gcgctggtag gcatgcgccg cggtgaggag ccgcgcctcg
     2581 gagaaatgcg caccgacgat ctgcagtccc acggggagct tgcccacgaa gccacaggga
     2641 ac
//
LOCUS       DATLKL010000780         2158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_9165, whole genome shotgun sequence.
ACCESSION   DATLKL010000780 DATLKL010000000
VERSION     DATLKL010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9165"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2154)
                     /gene="gltB"
                     /locus_tag="VM713_10585"
     CDS             complement(<1..2154)
                     /gene="gltB"
                     /locus_tag="VM713_10585"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HVH84748.1"
                     /translation="MKTLPAIEQLIVNCPLPIDEPAFNRRLFMARRLAEKALSADPAF
                     YVPSLSASTIVFKGMVMPEHLTRFYPDLEDPRLESSVAVFHQRFSTNTLPQWRLAHPY
                     RYLAHNGEINTIQGNRNWAQARGPLLRSPLLPDLQQLLPIVSLTGSDSQSLDNMLEVL
                     LLGGLDPLHAMRLLMPPAWHGIDTIDPDLRAFYEYYSVHMEPWDGPAGVVLTDGRYAL
                     CTLDRNGLRPARFCVTRDRCLTIASETGVWDYQPADVVRKGKLGPGDMIALDLETGEL
                     LASPDIDQILKGRHPYKRWLREGVRYLESDLVDARLAAEPMDRETLSLYQKMFNVTQE
                     ERDDIIRVLAVDENEAVGSMGDDTPMPVLSHQVRPLYDYFRQQFAQVTNPPIDPLRET
                     IVMSLQTQIGPECNIFVPAPGHARQVVLGSPILSQRKLRQILAIEAVTHEFLDLQYEP
                     AEGLKRAILRLCAQAEAAVREGKLVLLLSDRYLVKGRVPAHALLATGAVHQHLVKSGL
                     RCKCNLLVETGTAREPHHFACLIGYGATAVYPYMAYQVLFEMMRRGRVKLDFAARLEL
                     GRRYRAGLRKGLFKIMSKMGISTIASYRSSQLFEIVGLADEVVQLCFTGTESRVGGAD
                     FDDLAADSGELAARAWNPRESIPQGGLLKYVHGGEYHMYNPDVIAALQAAVISGDYAS
                     YREFARMVNERPPSTLRDLLSLKRALRPVPLEEVEP"
BASE COUNT          354 a          709 c          746 g          349 t
ORIGIN      
        1 cggctcgact tcctcgagcg ggaccgggcg cagcgcgcgc ttgagcgaca gaagatcgcg
       61 caacgtcgag ggcggccgct cgttgaccat tcgcgcaaac tcgcggtagc ttgcgtagtc
      121 cccggaaatc acggccgcct gcagcgccgc gatcacgtcc gggttgtaca tgtggtactc
      181 cccgccgtgc acgtacttga ggagcccccc ctgcgggatc gactcgcgcg ggttccaggc
      241 gcgcgcggcg agctcgcccg aatcggccgc caggtcgtcg aagtcggcgc cgccgacgcg
      301 gctctcggtg ccagtgaagc acagctggac gacttcgtcc gcgaggccga cgatctcgaa
      361 gagctgcgag ctccgatagc tcgcgatggt ggagatcccc atcttcgaca tgatcttgaa
      421 caggcccttg cgcagccccg cccggtagcg gcgcccgagc tcgaggcgcg cggcgaagtc
      481 gagcttgacg cgcccgcgac gcatcatctc gaacagcacc tggtacgcca tgtaggggta
      541 caccgcggtc gcgccgtagc cgatgagaca ggcgaagtga tgcggctcgc gcgccgtgcc
      601 cgtctcgacc agcaggttgc acttgcagcg caggcccgac ttcaccaggt gctggtgcac
      661 ggctcccgtc gcgagcagcg cgtgcgccgg cacgcgcccc ttgacgagat agcggtccga
      721 gagcagcagc acgagcttgc cttcgcgcac cgcggcctca gcctgggcgc acaggcgcag
      781 gatcgctcgc ttcaggccct cggccggctc gtactgcaga tcgaggaact cgtgggtcac
      841 cgcctcgatc gccaggatct gccgcagctt gcgctgcgag agaatcggtg agccgaggac
      901 aacctggcgc gcgtgccccg gggcggggac gaagatgttg cactcgggtc cgatctgggt
      961 ctgcagcgac atcacgatcg tctcgcgcag cggatcgatc ggcgggttgg tgacctgcgc
     1021 gaactgctgg cggaagtagt cgtacagcgg gcgtacctgg tgcgaaagca ccggcatcgg
     1081 cgtgtcgtcg cccatcgagc ccaccgcctc gttctcgtcc acggcgagca cgcggatgat
     1141 gtcgtcgcgc tcctcctgcg tcacgttgaa catcttctga tagagagaca gcgtctcgcg
     1201 gtccatgggc tcggccgcga gccgcgcgtc caccaggtcg ctctcgaggt aacgcacgcc
     1261 ctcgcgcagc catctcttgt agggatgccg gcccttcaat atctggtcga tgtcaggcga
     1321 ggcgaggagc tcgcccgtct cgaggtcgag cgcgatcatg tcgccgggtc cgagcttgcc
     1381 cttgcgcacc acgtccgcgg gctggtagtc ccacaccccg gtttcggagg cgatggtcag
     1441 acagcggtcg cgcgtgacgc agaagcgcgc cggacgcagc ccgttgcggt cgagcgtgca
     1501 cagggcatag cgcccgtcgg tgagcaccac gccggcgggc ccatcccatg gctccatgtg
     1561 caccgagtaa tactcataga aggcgcgcag atccggatcg atggtgtcga tcccgtgcca
     1621 cgccggcggc atcaggaggc gcatcgcgtg cagcgggtcg aggcctccca ggagcagcac
     1681 ctccagcatg ttgtcgaggc tctgcgagtc ggagccggtg agcgacacaa tgggcagcag
     1741 ctgctgcagg tccggcagga gcggtgaccg caagagcggt ccgcgcgctt gcgcccagtt
     1801 gcggttgccc tgaatggtgt tgatctcgcc gttatgagcc agataccggt acgggtgcgc
     1861 gaggcgccac tgcggcaggg tgttggtcga gaagcgctgg tgaaacaccg ccaccgagga
     1921 ctcgagtcgc ggatcctcga ggtcggggta gaagcgggtg aggtgctcgg gcatcaccat
     1981 gcccttgaag actatggtgc tcgccgagag gctcggcacg tagaacgccg gatcggcgct
     2041 cagcgccttc tccgcgagcc tgcgcgccat gaagagtcta cgattgaatg ccggctcgtc
     2101 gatgggcagc gggcaattga cgatcagctg ctcgatcgcg ggcagcgtct tcagggcc
//
LOCUS       DATLKL010000781         3474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_88348, whole genome shotgun sequence.
ACCESSION   DATLKL010000781 DATLKL010000000
VERSION     DATLKL010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88348"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1154)
                     /gene="rapZ"
                     /locus_tag="VM713_10590"
     CDS             complement(222..1154)
                     /gene="rapZ"
                     /locus_tag="VM713_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HVH84749.1"
                     /translation="MTEHTPVRIVILSGLSGSGKSVALHMLEDLGFYCIDNIPAALLT
                     PFISFTVRSPETTYERTAIGLDARNTAAEIASVPQLIDELKRSGLQVEVIFLIAGEDE
                     LLRRFAETRRRHPLSAANVDLREAMALERRLLEPVASAADLLIDTSRMGVHALREVIH
                     QRVEQRAAERLSITFESFGFKDGIPGDADFVFDARALPNPYWEPGLRNLTGRDAEVVR
                     FLETHTDVARLVADIARFMRARLPEYQASNRGYLTVAVGCTGGQHRSVYIVDRLAEDF
                     GAEFPNVTARHSALPDSGMFRVRALQMSETPTPA"
     gene            complement(1188..1508)
                     /gene="raiA"
                     /locus_tag="VM713_10595"
     CDS             complement(1188..1508)
                     /gene="raiA"
                     /locus_tag="VM713_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011332471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HVH84750.1"
                     /translation="MQLNVTGHHVEVTPSLRIYVAKKLERIGRHFDRVIDVHCVLTVE
                     KLRQKAEATLHVSGNAIHAHATEEDMYAAIDLLADRLDRSIKKHKEKRANMRAALGRR
                     APRA"
     gene            complement(1564..2997)
                     /locus_tag="VM713_10600"
     CDS             complement(1564..2997)
                     /locus_tag="VM713_10600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HVH84751.1"
                     /translation="MLKPSLQLKLGQQLTMTPQLQQAIRLLQLPALELQAHIRELLEK
                     NVMLEPVEETEGTGTFEVVAIPEPPPPPESRRESTVEVLDEPWSQQNTGPADTPWSGD
                     DEERQQDYADDAGQSLQDHLLWQLELARLAPRELAIASAIVDAISDDGYLTESLEEIA
                     QTLRPELEAAAGEVQAVLERVQALDPAGVGARSVGECIELQLRQLDPTTVGLETALKI
                     ARHHLELVAERELSALKRELRATEEEIACALALVRSCHPRPGATVSAGNAQYVVPDVF
                     VRRTDHGWGVEINPATLPRVRLNQSYASLIGRAASHASMRAQLQEARWLLKSLEIRHE
                     TLMKVARSIVERQTAFLEHGEEHMRPMILRDIAEAVAMHESTISRVTSGKYMHTPRGV
                     FELRYFFSSQVEGADGSGTSSTAIRAKIRKLVKDEDPENPLNDGRIAQLLSGEGISVA
                     RRTVAKYREAMRIPASSERRRAATHQL"
     gene            complement(3095..>3474)
                     /locus_tag="VM713_10605"
     CDS             complement(3095..>3474)
                     /locus_tag="VM713_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVH84752.1"
                     /translation="RLEQLLEELRIGHVRRTLGISLSGGERRRVEIARALAAEPRFML
                     LDEPFAGVDPLSVLDIQRIIRELTHRGIGVLITDHNVRETLGVCARAYIVNQGTVIAS
                     GSAEEVLANSQVREVYLGETFRL"
BASE COUNT          512 a         1117 c         1232 g          613 t
ORIGIN      
        1 agctcgcgag ctatctgacc ttcgagtgcg gctatacgcg cgcgctcatc gagctcggct
       61 accgcgacgc catggaagcg cgctcggcgc tcgtcgcttt catgagcggc gcgcggctgc
      121 cggcggtgct gacggcaccc ggagttgcgc aggcgacctg aggtacatgg tgaacacgcc
      181 gcgcttgcgc gtgcgcgacg tccccgggga gatcggacac ctcaggcagg cgtaggcgtt
      241 tcgctcatct gcagcgcgcg tacccggaac attccggaat ccggcagggc gctgtgccgc
      301 gccgtgacgt tgggaaactc ggcgccgaag tcctcggcga gccggtcgac gatgtagacc
      361 gagcggtgct gtcctccggt gcagcccacc gcgacggtga gatagccgcg gttgctcgcc
      421 tggtactcgg gcaggcgggc gcgcatgaag cgcgcgatat ccgcgaccag tctcgccacg
      481 tcggtgtgcg tctcgagaaa gcgcaccacc tcggcgtcgc gcccggtgag gttgcgcagg
      541 cccggctccc agtagggatt cggcagcgcg cgcgcatcga agacgaagtc ggcatctccc
      601 gggatgccgt ccttgaagcc gaacgattcg aaggtgatcg acagacgctc ggcggcgcgt
      661 tgctcgacgc gctggtgaat gacctcgcgc agcgcatgca cgcccatgcg cgaggtgtcg
      721 atcagcaggt cggcggcgga tgccaccggc tcgagcagcc ggcgctcgag cgccatcgcc
      781 tcgcgcaggt cgacgttggc ggcgctcagg gggtggcggc ggcgcgtctc ggcgaagcgc
      841 cgcagcagct cgtcctcgcc ggcgatgagg aagatcactt cgacctgcag tccgctgcgc
      901 ttcaactcgt cgatcagctg cggcacgctg gcgatctcag cggcggtatt gcgcgcatcg
      961 aggccgatgg cggtgcgctc gtaggtggtt tcggggctac gcaccgtaaa ggagatgaag
     1021 ggcgtgagga gcgcggcggg gatgttgtcg atgcagtaga acccgagatc ctcgagcatg
     1081 tgcagggcca cgctcttgcc ggacccggac aagccgctca ggatgacgat gcgcacgggg
     1141 gtatgttcag tcacccggcg ccgaggggca agcgcggccc ggtcgggtca ggcgcgcggt
     1201 gcgcgccgcc ccagggccgc acgcatgttg gcgcgttttt ccttgtgctt cttgatcgag
     1261 cggtcgagcc tgtcggccag caggtcgatg gccgcgtaca tgtcctcctc ggtggcatgg
     1321 gcgtggatgg cattgccgct cacgtgcagc gtcgcctcgg ccttctgtcg cagcttctcc
     1381 accgtcagga cacagtgcac gtcgatgacc cggtcaaagt gccggccgat gcgctcgagc
     1441 ttcttcgcca catagatacg cagcgatgga gtgacttcga cgtggtgccc ggtgacgttg
     1501 agctgcatgg tcggtctcct gttgatggtt ttcttccggt ggtggatttg cgcctgtcat
     1561 gcgtcagagc tggtgtgtgg ctgcgcggcg acgctcgctc gaggcgggga tgcgcatggc
     1621 ctcgcggtac ttggcgacgg tgcggcgcgc cacggagatg ccttcgccgg agagcagctg
     1681 ggcgatgcgg ccgtcgttca gcggattctc ggggtcctcg tccttgacga gcttgcggat
     1741 cttggcgcgg attgccgtcg aggaggtgcc ggagccgtcc gcgccctcga cctggctcga
     1801 gaagaagtag cgcagctcaa agactccgcg cggggtatgc atgtacttgc ccgacgtcac
     1861 ccgcgagatg gtcgactcgt gcatcgcgac cgcctcggcg atgtcgcgca ggatcatcgg
     1921 ccgcatgtgc tcctcgccgt gctcgaggaa ggcggtctga cgctcgacga tcgagcgcgc
     1981 caccttcatg agtgtctcgt ggcggatctc gaggctcttg agcagccagc gcgcctcctg
     2041 cagctgcgcg cgcatgctag cgtggctggc ggcgcgcccg atgaggctcg cgtaactctg
     2101 gttgaggcgt acacgcggca gcgtcgccgg gttgatctcc acgccccagc cgtggtcggt
     2161 gcggcgcacg aacacatccg gcacgacgta ctgcgcgttg ccggcgctca cggtcgcgcc
     2221 cggccgggga tggcatgagc gcaccagggc gagcgcgcac gcgatctcct cctcggtggc
     2281 gcgcagctcg cgcttcagcg ccgagagctc gcgttcggcg accagctcga gatgatgacg
     2341 ggcgatcttg agcgcggttt cgaggcctac cgtggtgggg tcgagttgcc gcagctgcag
     2401 ctcgatgcac tcaccgaccg agcgcgcacc gacgccggca gggtcgagcg cctgcacgcg
     2461 ctcaagcacg gcctgcactt ccccggcggc ggcctcgagc tcggggcgca gcgtctgcgc
     2521 aatctcctcg agcgactcgg tgagatagcc gtcgtcgctg atcgcgtcca cgatcgcgct
     2581 ggcgatcgcg agctcccgcg gcgcgagccg ggcgagctcg agctgccaca gcaggtgatc
     2641 ctgcagcgac tggcccgcgt cgtcggcgta gtcctgctga cgctcttcgt cgtcgccgct
     2701 ccagggcgtg tccgcaggcc cagtgttctg ctgactccag ggctcgtcca gcacctcgac
     2761 ggtgctctcg cggcggcttt ccggcggggg cggcggttcc gggatggcga ctacctcgaa
     2821 cgtgcccgtg ccctcggtct cctcgaccgg ctcgagcatc acattcttct cgagcagctc
     2881 gcggatgtgg gcctgaagct cgagcgccgg cagctgcagg agccggatgg cctgctgcag
     2941 ctgtggagtc atggtcagtt gctgaccaag cttcagctgc agggagggtt tcagcatggt
     3001 gccttgtgag acgacggggc gtcctggccc cgggttccta ttttcgtaat taactgaaaa
     3061 ataaggttta tctaggtgtg cgatccagac agcctcagag ccggaacgtc tcgcccaagt
     3121 acacctcacg gacttggctg ttggcaagga cttcttcggc acttccggac gcaatcaccg
     3181 tgccctggtt gacgatgtag gcgcgggcgc agacgccgag ggtctcgcgc acgttatggt
     3241 cagtgatgag cacgccgatg ccgcggtgcg tcaactcgcg aatgatgcgc tggatgtcga
     3301 ggacggagag ggggtcgacg cccgcgaagg gctcatcgag gagcatgaag cgcggttcgg
     3361 ccgccagcgc ccgtgcgatc tccacccgcc gccgctcgcc gccggataag gagatgccga
     3421 gcgtgcggcg cacgtgcccg atccgcagct cctcgagcag ttgctcgagc cgct
//
LOCUS       DATLKL010000782         3254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_31968, whole genome shotgun sequence.
ACCESSION   DATLKL010000782 DATLKL010000000
VERSION     DATLKL010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31968"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VM713_10610"
     CDS             <1..279
                     /locus_tag="VM713_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH84753.1"
                     /translation="EREKIARIRVEKCAHGVRISVADNGAGISAENMSRLFIHGFTTK
                     KTGHGFGLHSGALAAKEMGGVLRAESAGPGCGATFVLELPLKHPEKPQ"
     gene            276..2234
                     /locus_tag="VM713_10615"
     CDS             276..2234
                     /locus_tag="VM713_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVH84754.1"
                     /translation="MTSATRHVRRRLLVIDDNPAIHEDFKKILPGETQEVELLEAELL
                     EAEQLLLGEAKARTSRPTFEIDTALQGQEGVERVRQALAEGRPYAMAFVDMRMPPGWD
                     GLETIQELWKVDPHVQVVICSAHSDYDWTQVVAQLDHSDRLLVIKKPFEPIEVLQCAN
                     ALTRKWDNEHILRRQVEDLEQVVEARTQGLEAANKQLRHLATHDALTGLPNRVLLDDR
                     LTQAAAHAERDGHSFAVAMFDLDRFKVVNDSFGHRAGDQLIKEVAYRLAGIARGTDTV
                     ARLGGDEFLLIIDRLAKREEALQIVRRAVEALAQPIRLGDVDIHATASIGVAMFPVDG
                     KAVDTLLANADAAMYCAKQRGRNNIQSYTSGMNAVTQDKVKLESDLHAALTLNQLELH
                     FQPKIETASGAIHGAEALIRWRHPERGLVPPGEFIPLAEACGLINKIGEWVVREACRQ
                     ARAWQLQQLPPLRVAVNLSALQFRDGNLLHMIGEALAAAPLEARFLEVEITESALMSD
                     PEESVKILEQLSRMGVVVSVDDFGTGYSSMSYLRRFPIDKLKIDRSFVNELLSRADDA
                     SIVRAIVSLAHSLRLKVVAEGVETGEQLELLRKLGCDQYQGFCFSPAVPAPKFAELLV
                     ESRRKAEADTDLMAERTHSRLSFPRPRG"
     gene            complement(2263..>3254)
                     /locus_tag="VM713_10620"
     CDS             complement(2263..>3254)
                     /locus_tag="VM713_10620"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVH84755.1"
                     /translation="ETNMTDLALGFIGLGNVGGQLAGNLLRHGANLTVRDLDPERVAA
                     LVARGARAAASPRELAERVDLVITCLPSPAVSAAVMEAPDGVLAGLSAGKIWLEMSTT
                     DAAEARRLAARVEALGATAMDCPVSGGCHRAASGNISIFAGGSRAAFERVLPVLAILG
                     RRILHTGPVGSASVLKVITNYLCTVHLAALAEALTVAKVAGLDLNMTYEGIRISSGNS
                     FVHETESQVILNGSRDINFTMDLVVKDVGLFDALARAHRVPLELSPLVLGLFEDGARR
                     FGPREHSPNIIRRLEEACGVRVLGEGFPAQMVDDEPKVLGVEVVPHRRRAQPT"
BASE COUNT          564 a         1088 c         1116 g          486 t
ORIGIN      
        1 gagcgggaga aaatcgcccg gatccgcgtc gagaagtgcg cgcacggcgt acgcatatca
       61 gtggccgaca acggggcggg catttcggcc gagaacatga gccggctctt catccacgga
      121 tttaccacca agaagacggg ccacggattc ggcctgcaca gcggtgcgct ggccgccaag
      181 gagatgggcg gagtgctgcg tgccgagagt gccggtcccg ggtgcggggc caccttcgtt
      241 ctcgaacttc ccctgaaaca tcccgagaag ccccagtgac cagcgcgacc agacatgtca
      301 gacggcgatt actcgtcata gacgacaatc cggcgatcca cgaggacttc aagaagatcc
      361 tcccggggga gacccaggaa gtcgagctgc tcgaggccga gctgctcgaa gccgagcagc
      421 tgttactcgg tgaggccaag gcgcgcacgt cccggccgac tttcgagatc gacaccgccc
      481 tgcagggcca ggaaggagtc gagcgcgtca ggcaggcgct ggccgagggc cggccctacg
      541 cgatggcgtt tgtcgacatg cggatgcccc cgggttggga cgggctggag acgatccagg
      601 aactctggaa ggtcgacccg cacgtgcagg tggtcatctg ctcggcgcac tccgactacg
      661 actggaccca ggtcgtggcg caactggatc actccgaccg gctgctggtc atcaagaagc
      721 ccttcgagcc gatcgaggtg ctgcagtgcg ccaacgcgct gacccgcaaa tgggacaacg
      781 agcatatcct gcgccgtcag gtcgaggacc tcgagcaggt agtagaggcc cgcactcagg
      841 gcctcgaggc cgccaacaag cagctgcggc atctcgccac ccacgatgcg ctcaccgggc
      901 tgccgaaccg ggtgctgctc gacgaccgtc tgacgcaggc ggcggcgcac gccgagcgcg
      961 acggtcactc cttcgcggtc gccatgttcg atctcgaccg cttcaaggtc gtcaacgatt
     1021 ctttcggcca ccgcgccggg gaccagctca tcaaggaggt ggcgtatcgc ctcgcaggca
     1081 tcgcccgcgg caccgatacc gtggcgcgcc tcggcggcga cgaattcctt ctcatcatcg
     1141 accggctggc gaagcgcgag gaagcgctgc agatcgtgcg ccgcgcggtc gaggcgctgg
     1201 cgcagccgat ccggctcggt gacgtcgaca ttcatgccac ggcgagcatt ggggttgcga
     1261 tgtttccggt cgatggcaag gccgtcgaca ccctgctcgc caacgctgat gcggccatgt
     1321 actgcgccaa gcagcgcggc cgcaacaaca tccagtctta cacctcgggg atgaatgccg
     1381 tcacccagga caaggtgaag ctcgagagcg atctgcacgc cgcactgacg ctgaatcagc
     1441 tcgagctgca cttccagccg aagatcgaga ccgcgagcgg cgccattcac ggcgccgagg
     1501 cactgattcg ctggcgtcac ccggagcgcg ggctggttcc gccaggcgaa ttcattccgc
     1561 tcgcggaggc ctgcgggctc atcaacaaga tcggcgaatg ggtggtgcgc gaggcgtgcc
     1621 ggcaggcgcg cgcctggcag ctgcagcagc tgccgccgct gcgggtggcg gtgaacctct
     1681 cggcgctgca gttccgcgac ggcaacctgc tgcacatgat cggcgaggcg ctggcggccg
     1741 cgccactcga ggcgcgattc ctcgaggtgg agatcaccga gagcgcgctg atgagcgatc
     1801 cggaagagtc ggtgaagatt ctcgagcagc tcagccgcat gggtgtggtg gtgtcggtcg
     1861 acgacttcgg caccgggtat tcgagcatga gctacctgcg ccgcttcccg atcgacaagc
     1921 tgaagatcga tcggtcgttc gtcaacgagc tcctgtcgcg cgccgacgat gcctccatcg
     1981 tgcgtgccat cgtgtcgctg gcgcacagcc tgcgcctcaa ggtggtggcc gagggcgtcg
     2041 agaccggtga gcagctcgag ctcctcagga agctcggctg cgaccagtat cagggcttct
     2101 gcttcagccc ggcggtgccg gcgccgaagt tcgccgagct gctggtcgag tcacgcagga
     2161 aggcggaggc cgacaccgac ctcatggccg agcgcaccca cagcaggctc tcgttccctc
     2221 ggccgcgcgg ctagcgcgaa cgctcgccgc tccggctcgg attcaggtag gctgcgcgcg
     2281 gcgtcggtgc ggcaccacct cgacgcccag gaccttcggc tcatcgtcca ccatctgcgc
     2341 cgggaagccc tcgccgagta cccgcacgcc gcaggcctcc tcgaggcggc ggatgatgtt
     2401 gggggaatgc tcgcgcggcc cgaagcggcg cgcgccatcc tcgaacaggc cgagcaccag
     2461 cggcgagagc tcgagcggca cccgatgcgc gcgcgccagc gcgtcgaaca gacccacgtc
     2521 cttcaccacc aggtccatgg tgaagttgat gtcgcggctg ccgttcagga tcacctgcga
     2581 ctcggtctcg tgcacgaagg aatttcccga ggagatgcgg atgccttcgt aggtcatgtt
     2641 gaggtcgagg cctgccacct tcgccaccgt gagcgcctcg gcgagcgccg cgaggtgcac
     2701 cgtgcacaga tagttggtga tcactttgag gaccgaggcc gagcccaccg gacccgtgtg
     2761 caggatgcgc cgtccgagaa tggcgagcac cggcagcacc cgctcgaaag ccgcgcggct
     2821 ccccccggcg aagatcgaga tgttgccgct cgccgcgcgg tggcagccgc cggagaccgg
     2881 acaatccatc gcggtcgcgc ccagggcctc gacgcgcgcg gcgagccgcc gggcctcggc
     2941 ggcatcggtg gtgctcatct cgagccagat cttccccgcc gagaggccgg cgagcacgcc
     3001 gtccggcgcc tccatcaccg cggcggagac cgccggggaa ggcaggcagg tgatgacgag
     3061 atcgacgcgc tcggcgagct cgcgcgggga ggcggccgcc cgggctcccc gtgcgacgag
     3121 ggccgccacg cgctcagggt cgaggtcgcg caccgtaaga ttcgcgccgt ggcgcagcag
     3181 gtttccggcg agctgtccgc ccacgttgcc gaggccgatg aaaccgagtg cgaggtcagt
     3241 catgttggtt tcga
//
LOCUS       DATLKL010000783         2652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_223261, whole genome shotgun sequence.
ACCESSION   DATLKL010000783 DATLKL010000000
VERSION     DATLKL010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223261"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VM713_10625"
     CDS             complement(<1..488)
                     /locus_tag="VM713_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84756.1"
                     /translation="MKAARLALRILAREWRSGELGVLLLALTVAVAALTGVGFLVGRI
                     SAAVALQASSVLAADIRLSSPRALDPAYFAEAARRGLASSSTTSLLSVVFNVDANQLT
                     NVTAVTAGYPLRGRLLIAAEPFAPGTPAPGIPAAGEVWPASKLLAAVGGRVGSQLTIG
                     AAT"
     gene            complement(485..1192)
                     /locus_tag="VM713_10630"
     CDS             complement(485..1192)
                     /locus_tag="VM713_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003189864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVH84757.1"
                     /translation="MSSNATPSAQAPATLDSVLKAENLSKQVSSPEGALTIVDSVSLA
                     IAHGESVAVAGPSGAGKSTLLALLAGLDAPSRGRVLLEGTDLTSLDEDGRARLRAQRV
                     GFVFQSFHLIPALTALENVMLPLELAGRSDARRGATETLTLVGLKGRTGHYPRQLSGG
                     EQQRVALARAFVTRPAVLFADEPTGNLDTATGARVGELLFELNASARTTLVLVTHDRD
                     LAARCGRMLRMEAGRIT"
     gene            1134..1790
                     /locus_tag="VM713_10635"
     CDS             1134..1790
                     /locus_tag="VM713_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HVH84758.1"
                     /translation="MRTESRVAGAWALGVALLLIALGAAAASGRTILVFGDSLSAAYG
                     LAPQEGWVALLAQRLRSQGYGYQIVNASESGETSGGGLERLPRALALHHPGLVILELG
                     ANDGLRALPVTAMRANLERMVELARAAGAQVLLVGIRIPPNYGPRYTGEFERVFPDLA
                     NQYHLPLVPFLLEKVALDPELMQADGMHPNARGEAPVLDTVWPYLEPLLEKKHRTGGS
                     "
     gene            1790..>2652
                     /locus_tag="VM713_10640"
     CDS             1790..>2652
                     /locus_tag="VM713_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVH84759.1"
                     /translation="MDKIWLKAYPPHVPAEVDPARLRSLKQLLEETCAKHADRIAYLY
                     MGATITYRQLDTLSRAFGAWLQQAGFRKGDRIAIMLPNSPQYPIAMFGALRAGLIVVN
                     TNPLYTAPELAHQLTDAGAIAIVVLENFAHVVEKALPHTPLKTVLVTAVGDFLPFPKS
                     AIVNFVVRRVKKQVKPWHIASAASFKAAVSAGLKRPLAPVDLGPDDLAFLQYTGGTTG
                     VAKGAMLSHGNVSVNVLQAEAWIGESLPDHGTLLTAIPLYHIFALSCNCMVFARLGWQ
                     NLLIINPRDLKS"
BASE COUNT          422 a          927 c          908 g          395 t
ORIGIN      
        1 gtcgccgcgc cgatcgtgag ctgcgagccg acccggccgc cgactgcggc gaggagcttc
       61 gaggccggcc agacctcccc cgcggccggg atgcccggcg ccggcgttcc cggcgcgaag
      121 ggctcggcgg cgatgaggag ccggccgcgc agcggatagc ccgcggtcac cgccgtcacg
      181 ttggtgagct ggttggcgtc gacgttgaag actaccgaga gaagcgaggt ggtgctcgac
      241 gaggcgagtc cgcgccgcgc agcctcggcg aagtaggctg ggtcgagcgc gcgtggactg
      301 ctcaagcgga tgtcagccgc gagcaccgag ctcgcctgca gcgcgaccgc agcgctgatg
      361 cgcccgacca ggaagccgac cccggtgagc gccgcgactg ctacggtgag ggccagcagc
      421 agcacgccca gctcgcccga gcgccactcg cgcgcaagga tcctgagcgc cagtcgtgcg
      481 gccttcatgt gatgcgcccg gcttccatgc gcagcatccg gccgcagcgc gcggcgaggt
      541 cgcggtcgtg agtcacgagc accagcgtcg ttcgggcgct ggcgttcagc tcgaagagga
      601 gctccccgac ccgcgcgccg gtcgcggtgt cgagattgcc ggtcggctcg tccgcgaaca
      661 gcaccgccgg gcgggtgacg aaggcgcggg cgagcgcgac gcgctgctgc tccccgcccg
      721 agagctggcg cgggtagtgc ccggtgcgtc ccttgagccc gacgagggtg agcgtctcgg
      781 tggcgccgcg gcgtgcatcg ctccgacccg cgagctcgag cggcaacatc acgttctcga
      841 gcgcggtaag cgcgggaatc agatggaacg actggaacac aaagccaacc cgctgtgcgc
      901 gcagccgcgc gcgcccgtcc tcgtcgaggc tcgtgaggtc cgtaccttcg agcagcaccc
      961 gcccgcgact cggggcgtcg aggccggcca gcagcgcgag cagcgtcgac ttgccggccc
     1021 cggagggacc ggccacggcc accgactccc cgtgcgcgat cgcgagcgac acggaatcga
     1081 cgatggtcag cgcgccttcc ggactgctaa cctgcttgga gaggttttcg gccttgagaa
     1141 cagagtcaag agtcgctggc gcctgggcgc tcggcgtggc gttgctgctc atcgcgctcg
     1201 gggccgcggc ggcctccggc cgcacgatcc tcgtgttcgg tgacagcctg agcgccgcat
     1261 atggactcgc tccgcaggag ggttgggtcg cattgctcgc gcagcgactg cgctctcaag
     1321 ggtacggata ccagatcgtc aacgccagcg aaagcggcga gacgagtggt gggggcctcg
     1381 aacggttgcc gcgcgcgctg gcgctgcacc accccgggct cgtgatcctc gagctcggcg
     1441 ccaacgacgg gctgcgggcg ctgccggtta cggcgatgcg cgccaacctc gagcggatgg
     1501 tggagctcgc gcgcgcggcc ggagcgcagg tgctgctcgt cggcatccgt attccgccca
     1561 actacgggcc gcgctacacg ggggaattcg agcgcgtgtt cccagacctc gcaaaccaat
     1621 atcatctgcc gctggtgccg ttcctgctcg agaaggtggc gctcgacccc gagctcatgc
     1681 aggccgacgg catgcatccg aatgcccgcg gggaagcgcc cgtgctcgat accgtgtggc
     1741 cgtacctcga gccgctcctc gagaagaaac atcggaccgg gggaagctga tggacaagat
     1801 ctggctgaaa gcgtacccgc cgcacgtgcc cgcggaggtc gaccccgcgc ggctgcgctc
     1861 gctgaaacag ctcctcgagg agacctgtgc caagcacgcc gatcggattg cctatctcta
     1921 catgggcgcg acgatcacct accgacagct cgacactctg tcgcgtgcct tcggcgcgtg
     1981 gctgcagcag gcgggattcc gcaaaggcga ccgcatcgcc atcatgctgc cgaacagccc
     2041 gcagtatccg atcgccatgt tcggggcgtt gcgcgccggg ctcatcgtcg tcaacaccaa
     2101 cccgctctac accgcgccag agctcgcgca tcagctcacc gacgccggcg cgatcgccat
     2161 cgtggtgctc gagaacttcg cgcacgtagt ggagaaggcg ctgccgcaca ctccgctgaa
     2221 aaccgtgctc gtgaccgccg tcggcgactt cctccccttc ccgaagtccg cgatcgtcaa
     2281 tttcgtagtc cgccgcgtga agaagcaggt gaagccctgg cacatcgcga gcgcggcctc
     2341 cttcaaggcg gcggtgagcg caggtctcaa gcggccgctc gccccggtcg acttaggtcc
     2401 cgacgatctc gcgttcctcc agtacacggg cggcaccacc ggggtcgcca agggcgcgat
     2461 gctttcgcac ggcaacgtga gcgtgaacgt gctgcaggcg gaggcctgga tcggcgagag
     2521 cctcccggat cacgggacgc tgctcaccgc cattccgctc tatcacatct tcgcgctctc
     2581 ctgtaactgc atggtgtttg cgcgtctcgg ctggcaaaac ctcctcatca tcaatccgcg
     2641 cgacctcaag tc
//
LOCUS       DATLKL010000784         3577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_133646, whole genome shotgun sequence.
ACCESSION   DATLKL010000784 DATLKL010000000
VERSION     DATLKL010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133646"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VM713_10645"
     CDS             complement(<1..960)
                     /locus_tag="VM713_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HVH84760.1"
                     /translation="MTPRTWLPTAMAAATLLATATACTRSGSGPAGPGADRAPEGPVL
                     VRGGGQEPDSLDPQKVRGAETQGIVRDLCEGLTTLDKSAAVAPGVASRWSVSADGKVY
                     TFTLRPEARWSNGERVVAADFVAGLRRLVDPATASGYAQYADVILNTDDIVAGRKPPE
                     ALGVAAPDDSTVVVTLAAPAPYLPALLSHPSTCPVHRPTLARHPEGFARPGVMVADGA
                     FVLKEWVPGSHVLAVRNRYYWNDAATRLAAVKYLIITDENAELARYRSGELHITAVVP
                     RGQFDWIRQHLARELHVSPQLTTYYYGFNLRRAPFRDQPKLRRA"
     gene            complement(957..1799)
                     /gene="pyrF"
                     /locus_tag="VM713_10650"
     CDS             complement(957..1799)
                     /gene="pyrF"
                     /locus_tag="VM713_10650"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HVH84761.1"
                     /translation="MSAFTRSLARAWQTNDSLLCVGLDPEIERFPPHIAAAPSPIFQF
                     NKAIVDATHDLVCAYKPQFAHYAAYEAEDQLERTIEYIHRTYPGIPVILDGKRGDVGN
                     TAERYAIEAFERYAADAVTVNPYLGGDSLEPFLRHADKGVVILCRTSNPGARDLQDLE
                     VGGRRLYQAVAELAATRWNSNGNCALVVGATYPRELAEVRRIVGEMPLLLPGVGAQGA
                     DVAQAVEAGETADGTGLMVSSSRGILYGTGREPGADFAAAARAAAEALRTQINSSRRR
                     RGSP"
     gene            complement(1796..>3577)
                     /gene="mnmG"
                     /locus_tag="VM713_10655"
     CDS             complement(1796..>3577)
                     /gene="mnmG"
                     /locus_tag="VM713_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HVH84762.1"
                     /translation="IGKGHLVRGIDALGGAMGRAADRAGIQFRTLNASKGPAVRATRA
                     QADRQLYRTAIRSSLESQPRLALFQQEAADLLVEGERVCGVVTVTGIEIRARAVVLTV
                     GTFLGGRIHVGLDNYPGGRAGDPASLRLAARLRDFGLRVGRLKTGTPPRIDGRTLNWS
                     VMTRQPGDVPTPVFSFLGRVEDHPRQVDCFITHTNERTHEIIRGATDRSPMFTGVIEG
                     TGPRYCPSVEDKVVRFAQKPSHQIFVEPEGLNTHEIYPNGISTSLPYDVQVEFVRTIR
                     GFENAHLTRPGYAIEYDYFDPRDLNGSLETRALAGLYFAGQINGTTGYEEAAAQGLVA
                     GINAARAVAGQEPWQPKRSEAYLGVLIDDLVTRGTREPYRMFTSRAEHRLLLREDNAD
                     ARLTPIGRELGLVDPERWRLFEIKQYLTELEIERLTELRVKAAQLSPDWAARVLGAPL
                     AQDTSAFELLKRPGVGYQALLDVAGPPEWLPEGDDEEAVLPIDARLPDQIRTQVEVRA
                     RYAGYLERQQEEIERTARNEETRLPPDLDYRKLAGLSHEVRQRLAEACPATLGQAGRI
                     PGVTPAAVSILLVHLKKRGARSAPRVA"
BASE COUNT          479 a         1221 c         1338 g          539 t
ORIGIN      
        1 cgcgcggcgc agcttcggct ggtccctgaa aggcgcccgc cgcaggttga agccgtagta
       61 gtaggtcgtg agctgcggcg agacgtggag ctcgcgcgca agatgctgcc ggatccagtc
      121 gaactgcccg cgcggtacca cggcggtgat gtgcagctcg ccgctgcggt agcgggcgag
      181 ctctgcgttc tcgtcggtaa taatgaggta cttcaccgcg gcgagtcgcg tggccgcatc
      241 gttccagtag tagcggttgc ggacggccag cacgtgcgag cccggcaccc actccttgag
      301 cacgaaggcc ccgtccgcga ccatgacgcc gggtcgcgcg aagccttccg ggtggcgcgc
      361 gagcgtcggg cgatgcaccg gacaggtgct cgggtgcgag aggagcgcgg gcagatacgg
      421 cgcgggagct gcgagcgtca ccaccacggt cgagtcgtcg ggtgccgcga cgccgagcgc
      481 ctcggggggc ttgcgcccgg cgacgatgtc gtcggtattg aggatcacgt cggcgtactg
      541 cgcgtagccg gaggcggtcg ccggatcgac cagacggcgc aggcccgcca cgaaatctgc
      601 cgcgaccacc cgctccccgt tcgaccagcg ggcctcaggc cggagcgtga acgtgtagac
      661 cttgccgtcg gcgctcacgc tccagcggct cgccacgccg ggcgcaactg ccgcgctctt
      721 gtcgagggtc gtcaggccct cgcacaggtc ccgcacgatg ccctgcgtct ccgcgccgcg
      781 caccttctgc ggatcgagcg agtcgggctc ctggcctccg ccccgcacca acacgggccc
      841 ttcgggcgcg cggtccgcgc cgggcccggc tgggccgctg ccgctgcgcg tgcaggcggt
      901 ggcggtcgcg agcagcgtcg cggctgccat ggccgtcggg agccaggtgc ggggggtcat
      961 ggggagccgc gccgcctgcg gctcgagttg atctgggtcc gcagcgcttc ggccgcggcg
     1021 cgcgcggcgg cggcgaagtc ggccccaggc tcacgccccg tgccatagag aatgccgcgc
     1081 gaggagctga ccatgaggcc ggtgccgtcc gccgtctcac cggcttcgac cgcctgcgcg
     1141 acgtccgccc cctgtgcgcc gaccccgggc aggagcagcg gcatctcgcc cacgatgcgg
     1201 cgcacctcgg cgagctcgcg cggataggtg gcgccgacca ccagcgcgca gttgccgttg
     1261 ctgttccagc gcgtggcggc gagctccgcg accgcctgat acaggcgccg cccgccgacc
     1321 tcgaggtcct gcagatcccg cgcgccggga ttggaggtgc ggcagaggat cacgacgccc
     1381 ttgtcggcgt ggcgcagaaa cggctcgagt gagtcacccc cgagataggg attcacggtc
     1441 accgcatccg ccgcgtagcg ctcgaaggcc tcgatggcgt agcgctcggc ggtgttgccg
     1501 acgtcgccgc gcttgccgtc gaggatcacg gggattccgg ggtaggtgcg gtgtatgtat
     1561 tcgatcgtgc gctcgagctg gtcttcggcc tcgtaggcgg cgtagtgcgc gaactgcggc
     1621 ttgtaggcgc acaccaggtc gtgggtggcg tcgacgatcg ccttgttgaa ctggaagatc
     1681 ggcgagggcg cggcggcgat gtgcggcgga aagcgctcga tctcggggtc gagccctaca
     1741 cacaggagcg agtcgttcgt ttgccaggcg cgcgcgagcg agcgcgtgaa ggcgctcatg
     1801 cgacgcgcgg tgcgctgcgc gcgccgcgct tcttcaggtg cacgagcagg atcgacacgg
     1861 cggccggggt cacgccgggg atgcggcccg cctggccgag cgtagcggga cacgcctcgg
     1921 cgagccgctg gcgcacctcg tgcgagagcc cggcgagctt acggtagtcg aggtcgggcg
     1981 ggagccgcgt ctcctcgttg cgcgcggtgc gctcgatctc ttcctgttgc cgctcgaggt
     2041 agccggcgta acgggcccgc acttcgacct gggtgcggat ctggtcgggg aggcgcgcat
     2101 cgatcgggag gacggcctcc tcgtcgtcgc cttcggggag ccactcgggc ggacccgcca
     2161 cgtcgaggag cgcctggtag cccaccccgg ggcgcttgag cagctcgaac gcggaggtgt
     2221 cctgggcgag cggcgcgccg agcacccggg cggcccagtc cggggagagt tgcgcggcct
     2281 tcacccgcag ctccgtgagg cgctcgatct cgagctcggt gaggtactgc ttgatctcga
     2341 agagtcgcca gcgctcgggg tcgacgaggc cgagctcgcg cccgatcggc gtgaggcggg
     2401 cgtcggcgtt gtcctcgcgc agcagcagcc gatgctcggc gcggctggtg aacatgcggt
     2461 acggctcgcg cgtaccgcgt gtcacgaggt cgtcgatgag cacgccgagg tacgcctcgc
     2521 tgcgcttcgg ctgccagggc tcctggcccg cgaccgcgcg cgcggcattg atgccggcga
     2581 cgaggccctg ggcggcggcc tcctcgtagc cggtggtgcc gttgatctgc ccggcgaagt
     2641 agaggcccgc gagcgcgcgc gtctcgagcg agccgttcag gtcccgcggg tcgaaatagt
     2701 cgtactcgat cgcgtagccg ggacgcgtga ggtgggcgtt ctcgaagccg cggatcgtgc
     2761 gcacgaactc gacctgcaca tcgtacggca ggctcgtcga gatcccgttc gggtagatct
     2821 cgtgggtgtt gagcccctcg ggctcgacga agatctggtg cgagggcttc tgcgcgaagc
     2881 gcacgacctt gtcctcgacg gacgggcaat agcgcgggcc cgtaccctcg atcaccccgg
     2941 taaacatggg tgagcggtcc gtggcgccgc ggatgatctc atgcgtccgc tcgttcgtat
     3001 gcgtaatgaa gcaatccacc tgccttggat gatcctcgac ccgcccgagg aacgagaaga
     3061 cgggtgtcgg cacatcgccc ggctggcggg tcatcaccga ccagttgagc gtgcgaccgt
     3121 cgatccgcgg gggcgtcccg gtcttcaatc gtccgacccg caggccgaag tcgcgcagcc
     3181 gcgccgcgag gcgcagggac gcggggtcgc cggcgcgccc gcccgggtag ttgtcgagcc
     3241 cgacgtggat gcgtccgccg aggaaagtgc cgaccgtgag caccaccgcc cgggcgcgga
     3301 tctcgatccc ggtgaccgtg acgacgccgc agacccgctc cccctcgacc aggagatcgg
     3361 ccgcctcctg ctggaacagc gcgagccgcg gctggctctc gaggcttgag cggatcgccg
     3421 tccgatacag ctggcggtcg gcctgcgcgc gcgtggcgcg cacggccggt cccttgctgg
     3481 cgttcagcgt gcgaaactgg atgccggcgc ggtcggcggc ccgccccatg gcgcccccga
     3541 gggcgtcgat cccgcgcacc aggtgtccct tgccgat
//
LOCUS       DATLKL010000785         1731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_122299, whole genome shotgun sequence.
ACCESSION   DATLKL010000785 DATLKL010000000
VERSION     DATLKL010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122299"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VM713_10660"
     CDS             complement(<1..198)
                     /locus_tag="VM713_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84763.1"
                     /translation="MWLHRAAAIVVLSALTLGSAIGRSDDTSKAPPPLPAAGFLEFLG
                     SVDRLSELNPNYLPPPGAAKVG"
     gene            complement(198..599)
                     /locus_tag="VM713_10665"
     CDS             complement(198..599)
                     /locus_tag="VM713_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84764.1"
                     /translation="MSRVEESLTQFERNARVVLEEGVRRIDARTRSRLNQARQAALAR
                     IRARRRAWWSGLTLMPAAGALAAALLVAVVLWHREPQGTPPLALDGQHPTVEDMELLT
                     DNDSIDLIEGWDGSFYEWAAAESDAGAESNS"
     gene            complement(596..1222)
                     /locus_tag="VM713_10670"
     CDS             complement(596..1222)
                     /locus_tag="VM713_10670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVH84765.1"
                     /translation="MPEFATLASPYSMPAALHGDVDLTDPEEQTQTRALNQFLAGVEL
                     KAFKIAQAALRHEDDALDAVQDAMLQLARAYGHRPPGEWKPLFYRILENRIRDMQRRR
                     TVRGRVMAWLPSRGEDDDEDFDPVAQAPSLEPQPVRRLELDEAVGALEQALERLPRRQ
                     QQAFLLRTLEGLDVAETAQAMGCSEGSVKTHYFRAVQALRAQLGELYL"
     gene            1285..>1731
                     /locus_tag="VM713_10675"
     CDS             1285..>1731
                     /locus_tag="VM713_10675"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="binding of PriA to forked DNA starts the assembly
                     of the primosome, also possesses 3'-5' helicase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HVH84766.1"
                     /translation="MRPNPQFFRVAVDTPLRRLFDYLPPAQASGDAEPRPGTRVRVPF
                     GRRRMIGVVMGVSGTSDLPAERLKPILEVLDAAPPLEASALALIQWAAEYYHHPVGQV
                     LASALPRLMRLGAGVAATEERWTVTDAGREAAAAGEPRRAARQRAVL"
BASE COUNT          241 a          616 c          595 g          279 t
ORIGIN      
        1 ccctaccttc gccgcacccg gcggcggcag gtagttcggg ttcaactccg agagccggtc
       61 gacgctgccg aggaactcga ggaagcccgc ggccggcagc ggcggtggtg ccttgctcgt
      121 atcgtccgag cggccgatgg cgctgccgag cgtgagtgcg ctcagtacca cgatcgcagc
      181 ggcgcgatgg agccacatca gctgttgctc tcggctccgg catcggactc cgcggccgcc
      241 cattcgtaga acgagccgtc ccaaccctcg atcaggtcaa tcgaatcgtt gtctgtcagc
      301 agctccatgt cctcgaccgt gggatgctgc ccgtcgaggg ccagtggcgg cgtgccctga
      361 ggctcgcgat gccacagcac cacggccacg agcagcgccg cggccaaggc acccgcggcc
      421 ggcatcagcg tgaggccgct ccaccaggcg cggcggcgcg cgcgaatgcg cgcgagcgcg
      481 gcctggcgcg cctggttcaa gcgcgagcgc gtgcgcgcgt cgatgcgccg cacgccctcc
      541 tcgagcacca ctcgtgcgtt gcgctcgaac tgtgtcagtg actcctcgac ccggctcata
      601 agtagagctc tccaagttgc gcgcgcagtg cctgcactgc gcgaaagtaa tgagtcttca
      661 cgctgccctc cgagcagccc atcgcctgcg ccgtctcggc cacgtcgagg ccctcgagcg
      721 tgcgcagcag gaatgcctgt tgctgcctgc gcggcagccg ctcgagcgcc tgctcgagtg
      781 cgcccacagc ctcatcgagc tcgagccggc ggaccggctg cggctcgagg ctcggtgcct
      841 gagccacggg gtcgaagtcc tcgtcatcgt cttcgccgcg cgacggcagc catgccatga
      901 cgcggccccg cacggtgcgc cggcgctgca tgtcgcggat gcggttctcg agaatccggt
      961 agaacagcgg cttccactcc ccgggcggac ggtggccata ggcccgcgcc agctgcagca
     1021 tcgcatcctg gaccgcatcg agggcgtcgt cctcgtgccg cagcgccgcc tgggcgatct
     1081 tgaatgcctt cagctcgacg ccggccagga actggttcag cgctctagtt tgtgtttgct
     1141 cttcggggtc cgtcaggtcc acgtcgccgt gcagtgcggc cggcatgcta taaggcgagg
     1201 cgagcgtagc aaactcgggc atggcctgta taacgcgcgc acgcggcgtc cggttgacgc
     1261 tcaagccact gatttggctc atctgtgaga cctaatcccc agtttttcag ggtggcggtc
     1321 gacacccccc tgcggcggct cttcgactac ctgccgccgg cgcaggcgtc gggggatgca
     1381 gagccacggc cgggaacgcg cgtgcgggtg cccttcggcc ggcgccggat gatcggggtg
     1441 gtcatgggcg tctcgggcac ttcggacctg ccggccgagc gcctcaagcc catcctcgag
     1501 gtgctggatg cggcgccgcc gctcgaagct tccgccctcg cgctcatcca gtgggcggcg
     1561 gagtattacc accatcccgt cggtcaggtg ctcgccagcg cgttgccgag actcatgcgt
     1621 ctcggggccg gggtcgcggc gaccgaggag cgctggacgg tgaccgacgc cggacgcgag
     1681 gctgccgcgg ccggcgagcc gcgccgcgcg gcacgccagc gcgccgttct c
//
LOCUS       DATLKL010000786         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_31991, whole genome shotgun sequence.
ACCESSION   DATLKL010000786 DATLKL010000000
VERSION     DATLKL010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31991"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..993)
                     /locus_tag="VM713_10680"
     CDS             complement(100..993)
                     /locus_tag="VM713_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVH84767.1"
                     /translation="MRLSQATGRRLIAGVPTLWLLVLFLIPFVIVFRISFSEVRLAIP
                     PYTPLVSWHHGAPHFEVHWSAYSFLFTDPMYVSSYLYSLKVAAVSTLCCLLIGYPMAY
                     AIARAGPATRPLLLMLIVLPFWTSFLLRVYAWIGLLKNNGVINNVLLALGVIHHPLAL
                     LQTDFAVYIGIVYSYLPFMILPLYANLEKHDAALLEAAADLGARPWRAFARITLPQSL
                     PGIAAGSLLVFIPAVGEYVIPTLLGRPDQLMIGRVLSDEFFENRDWPVASAVAIIILL
                     LLVVPILVFQRYQRRELEGVA"
     gene            complement(1003..2157)
                     /locus_tag="VM713_10685"
     CDS             complement(1003..2157)
                     /locus_tag="VM713_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVH84768.1"
                     /translation="MSSQPTPAAAQSAASTPDSAYVRIRDVTKKFGDFTAVDHVSLDI
                     RRGEIFCLLGGSGSGKTTLLRMLAGFETPTTGRIEIDGQDLSGVPPYERPVNMMFQSY
                     ALFPHMSVEKNVAFGLEQERLGRDAIRRQVGEILEIVKMGSYGARKPHQLSGGQRQRV
                     ALARALVKRPKLLLLDEPLAALDRKLREHTQFELLNIQQRLGVTFIVVTHDQEEAMTL
                     GTRLGVMNHGRIVQVGTPSDIYESPATRFVADFIGSVNMFEGEVCANGADGLCIRSAD
                     LGCTVRAVRAPGGTRGATVWAAIRPEKIQIAREPLAGEDASPGENLTRGTVKEIAYMG
                     DMSIYLVRLDSGSMLRVTLPNVLRGAERSIAREERVWLSWHGSSPVVLTE"
BASE COUNT          416 a          749 c          807 g          333 t
ORIGIN      
        1 gggagttgtt gaaggagaac accatcatcg agaggattgg ccggtacagg aacgcgagcc
       61 ccaggacgag ggcgatacgc gcgaacaggc cccgcggctt catgcgaccc cctcgagctc
      121 gcgccgctgg tagcgctgga acacgaggat cggcaccacc agcagcagca ggatgatgat
      181 cgcaaccgcg ctcgccaccg gccagtcgcg attctcgaag aactcatccg agagcacccg
      241 gccgatcatc agctggtccg ggcgcccgag gagcgtcggg atcacgtact cgcccaccgc
      301 cgggatgaac accaggagcg agcccgcggc gatcccggga agggactgcg ggagcgtgat
      361 gcgcgcaaag gcgcgccacg ggcgggcccc caggtccgcg gccgcctcga ggagcgctgc
      421 gtcgtgcttc tcgaggttgg cgtacagcgg caggatcatg aacggcaggt acgagtagac
      481 gatgccgatg tagaccgcga agtcggtctg cagcagcgcg agcgggtgat ggatgacgcc
      541 gagcgcgagc agcacgttgt tgatcacgcc gttgttcttc agcagcccga tccaggcgta
      601 cacgcgcagc aggaaggagg tccagaacgg cagcacgatc agcatcagga gcagcggacg
      661 cgtggcgggc ccggcgcgcg cgatcgcata ggccatcgga tagccgatga gaagacagca
      721 cagtgtcgag accgcggcca ccttgaggga atacaggtac gagctcacgt acatcgggtc
      781 cgtgaacagg aacgagtacg ccgaccagtg cacttcgaag tgcggggcgc cgtggtgcca
      841 gctgacgagc ggcgtgtatg gcgggatcgc caggcgcacc tcggagaagg agatgcggaa
      901 cacgatcacg aaggggatca ggaacaacac cagcagccag agcgtcggca ctcccgcgat
      961 cagccggcgg cccgtggcct gtgacaaacg cactgcctcc ccctactcgg tgagcaccac
     1021 cgggctcgag ccgtgccagg agagccacac ccgctcctcg cgggcgatcg agcgctcggc
     1081 accgcgcagc acgttcggca gcgtcacccg cagcatgctg cccgagtcga ggcgcacgag
     1141 gtagatcgac atgtcgccca tgtaggcgat ctccttgacc gtaccccggg tgagattctc
     1201 gcccggcgag gcgtcttccc ccgcgagcgg ctcgcgcgcg atctggatct tctccgggcg
     1261 aatggcggcc catacggtgg cgccgcgcgt gccgccgggt gcgcgcaccg cgcgcaccgt
     1321 gcaccccagg tcggcgctgc ggatgcacag gccgtcggcg ccgttcgcgc aaacctcacc
     1381 ctcgaacatg ttgaccgagc cgatgaagtc ggcgacgaag cgcgtggctg gggactcgta
     1441 gatgtcgctc ggcgtaccga cctggacgat gcggccgtgg ttcatgaccc cgagccgcgt
     1501 gccgagcgtc atcgcctcct cctggtcgtg ggtcaccacg atgaaggtca ctccgaggcg
     1561 ctgctggatg ttgagcagct cgaactgggt gtgctcgcgc aatttcctgt cgagggcggc
     1621 gagcggctca tcgaggagga ggagcttcgg gcgcttcacc agcgcgcgcg ccagggccac
     1681 gcgctggcgc tggcctccgg agagctgatg cggcttgcgt gctccgtagc tgcccatctt
     1741 cacgatctcg agaatctcac ccacctggcg gcggatggcg tcgcgtccga ggcgctcctg
     1801 ctcgagaccg aaggccacgt tcttctcgac gctcatatgc ggaaagagcg catacgactg
     1861 gaacatcatg ttcacgggac gctcgtaggg gggcacgccg ctcaagtcct gcccatcgat
     1921 ctcgatccgg ccggtggtgg gcgtctcgaa gccggccagc atccgcagca gcgtcgtctt
     1981 ccccgagccc gaccctccaa gcaggcagaa gatctcgcca cggcggatgt caagcgatac
     2041 gtggtcgact gcggtgaaat caccgaactt cttggtgacg tcgcggatgc ggacgtaggc
     2101 ggagtcgggg gtggaggccg cggactgggc cgcggcgggc gtaggttgcg agctcacggg
     2161 ggaacgttac cttccagtct tgaaccgcgt ccacatgcgg gtgaggagcc gctggtattc
     2221 cggaggccgc gcgcgctccg gcgtgagccg ggcgcgcacc tcggcgggcg ggtacacgcc
     2281 gggatcgctc accacggcgg cctcc
//
LOCUS       DATLKL010000787         2739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_167431, whole genome shotgun sequence.
ACCESSION   DATLKL010000787 DATLKL010000000
VERSION     DATLKL010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167431"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VM713_10690"
     CDS             complement(<1..537)
                     /locus_tag="VM713_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84769.1"
                     /translation="MRLHAVLSLPVVCLAIGTVWAASDPFVGKWKVNPSKSRLTDEMK
                     VEAVGENKYAITFGPGAVDTIVADGTEQPALQGTTLSVTVEGPNNWKVVRKKEGRKVV
                     SAIWTLSRDGRTLDDAFTGYQPDGSPFNVHYIYERAAGSSGFSGTWDSVSAELLDSSI
                     ELEIQPYEGDGLSFRSGGL"
     gene            1033..2127
                     /locus_tag="VM713_10695"
     CDS             1033..2127
                     /locus_tag="VM713_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVH84770.1"
                     /translation="MAAAISRKITDFDELTGRFEAVERVEVRPRVSGYIASVNFKEGS
                     DVRKGDVLFVIDPRPYQAERDKAAAGLAQARSQLVLARTERERGIKLLAQHAISQEEY
                     DTRTAGSEQAEANVEAAKAALDATALNVEFTRVTAPISGRISRALVTSGNFVTNGQTP
                     LTTLVSLDPIYVSFDGDEQQYLKYSRLAREAAAGGVHEARNPVQVGLADESGFPHEGV
                     MVFVDNALDPATGTIRSRALLDNHERQFTPGLFARIKLIGSVSHDAVLVNDSAIGTDQ
                     TMRYVLVVDKSNKVELRPVQLGSIVDGLRVVQAGLSPGETIVVNGLQRVRPGAQVQPQ
                     RVAMGESRSGERPSLLARNAPGSGHSEAHP"
     gene            2124..>2739
                     /locus_tag="VM713_10700"
     CDS             2124..>2739
                     /locus_tag="VM713_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH84771.1"
                     /translation="MNISRFFIERPIFAGVISAFITIGGLLALFKLPISEYPEVVPPQ
                     VIVHASYPGANPKVIADTVAAPLEQAIVGVEDMLYMSSTSTMDGALTLTVTFKIGADI
                     DRAQVQVQNRVSQALPRLPQEVRDLGVNTVKASSNLTLVVNLTSPSGRYDSLYLRNYA
                     VLNIRDVLARLPGMGDVQVFGGGDYSMRVWLDPQKLAARNLTAGD"
BASE COUNT          499 a          906 c          852 g          482 t
ORIGIN      
        1 cagaccgcca ctgcgaaagg aaagaccgtc gccctcgtat ggctggatct cgagctcgat
       61 gctcgaatcc aacaactccg cgctcacgct atcccaagtg ccggaaaagc ctgagctgcc
      121 cgcggctcgc tcgtagatat agtgcacgtt aaacggtgaa ccgtcgggct ggtagcctgt
      181 aaaagcgtca tcgagcgttc tgccgtccct ggaaagtgtc caaatcgccg acactacctt
      241 ccgcccctcc ttcttgcgga cgaccttcca gttgttcggt ccttccaccg taacggacag
      301 ggtggttccc tgcagggcgg gctgctcagt cccgtcggct acgatcgtgt cgactgcacc
      361 gggaccaaag gtgatggcat atttgttttc accgacggct tcgaccttca tttcgtcggt
      421 gagtctgctc ttggatgggt tcaccttcca tttgcctacg aaggggtcgc tggctgccca
      481 taccgtgccg atagctagac aaacaaccgg cagtgacaga acagcatgta atcgcatggg
      541 catctccgaa ccctgtcggc gggcggtctc gatcagccga cgctagtccg cgaaaaacag
      601 ggtgtccaga cggagcggcg ttccccgcga cgaacggacg tctatgtacg cccaaactta
      661 agtcgcacca agccgcggtg cttacccgta tggatcaccc cgctcggagc cctgtccgca
      721 aggcttcctg cggcccaaag tccgcactgc ctcatccgag agagctgcac ccctgctcta
      781 tgaaacgccc ggggcgcccc ggttgatgca tatcaagccc ggcttgcgta ctgaagaatg
      841 cgtcgggcga tcacccatac gggcgggcgc tgggattgca cttggacaaa cagaagggag
      901 gaccccgtgc gtcattcttt tctatctctc gtagcggccg ctgccgtttc agcgactgcc
      961 ctcagtctcg gcggatgcgg gcggaacgag gccgcggagg cgcacgctgc gccgccggcg
     1021 ccgcaggtaa ccgtggccgc agcgatctcg cgcaagatca cggacttcga cgagctcacc
     1081 ggacgctttg aggcggtcga gcgcgtcgag gtgcgtccgc gtgtttccgg ctacatcgcc
     1141 tcggtcaact tcaaggaagg gagcgatgtc agaaagggcg acgtgctgtt cgtcatcgac
     1201 ccccgcccct atcaggcgga gcgcgacaag gcggcggcag gtctcgcgca ggcgcgctcg
     1261 cagctggtcc ttgcgaggac cgagcgcgag cgcggtatca agctcctcgc gcagcacgcc
     1321 atctcgcagg aagagtatga cacccgtacg gccggcagcg agcaggcgga ggccaacgtc
     1381 gaggccgcca aggcagcgct ggacgcgacg gccctcaacg tcgaattcac gcgcgtcact
     1441 gcgccgatct ccggacgcat cagccgggcg ctcgtcacca gcggcaattt cgtcaccaac
     1501 gggcagacgc cgctcacgac gctggtatcc ctggatccga tctacgtgag cttcgacggc
     1561 gacgagcagc agtatctcaa gtattccagg ctcgcccgcg aagcggccgc gggtggcgta
     1621 cacgaagcgc gcaacccggt gcaagtgggc ctggcggatg agagcggctt cccgcatgaa
     1681 ggcgtcatgg tgttcgtgga caatgccctc gacccggcga ccggcaccat ccgcagccgc
     1741 gcgctgctcg acaatcacga gcggcagttc acgccgggtc tcttcgcccg catcaagctc
     1801 atcggcagcg tctcgcacga tgcggtgctg gtgaacgaca gcgccattgg cacggatcag
     1861 accatgcgct acgtgctggt cgtcgacaag agcaacaagg tcgagttgcg ccccgtgcag
     1921 ctcggctcga tcgtcgacgg actgcgtgtc gtgcaagcgg gactctcgcc cggcgaaacc
     1981 atcgtcgtga acggcctgca gcgcgtgcgt cccggagcgc aggtgcagcc gcagcgggtc
     2041 gcgatgggtg aaagccgctc gggcgagcgc ccgtcgctgc tcgcccgcaa tgccccaggc
     2101 tctgggcaca gcgaggctca tccgtgaata tttcccgctt cttcatcgag cggcctattt
     2161 tcgccggagt catctcggcg ttcatcacca tcggcggact gctggcgctc ttcaagctgc
     2221 cgatcagcga gtaccccgag gtggtgccgc cgcaggtgat cgtgcacgcg tcgtatccgg
     2281 gcgcgaaccc taaagtgatc gccgatacgg tggcggcgcc gctggagcaa gcgattgtag
     2341 gcgtcgagga catgctctac atgtcctcga cttccaccat ggacggcgcg ctcacgctca
     2401 ctgtcacctt caagatcggc gccgatatcg accgcgcgca ggtccaggtg cagaaccgcg
     2461 tctcgcaggc actgccgcga ctgccacagg aggtccgcga ccttggcgtc aacaccgtca
     2521 aggcctcatc gaacctcacg ctggtcgtca acctcacttc acccagcggg cgctacgact
     2581 ctctctatct gcgcaactat gcggtgctga acatcaggga cgtgctggcg cgtctcccgg
     2641 gcatgggaga cgtgcaggtg ttcggcggcg gggactactc gatgcgcgtg tggctcgatc
     2701 cgcagaagct cgccgcgcgc aacctgaccg ccggcgatg
//
LOCUS       DATLKL010000788         1731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_178648, whole genome shotgun sequence.
ACCESSION   DATLKL010000788 DATLKL010000000
VERSION     DATLKL010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178648"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1666)
                     /gene="rpoC"
                     /locus_tag="VM713_10705"
     CDS             complement(<1..1666)
                     /gene="rpoC"
                     /locus_tag="VM713_10705"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004666357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HVH84772.1"
                     /translation="MKDLLKLFKQHAPVEHFDSIKIGLASPDKIRQWSHGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICGRCGVEVTQSKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLMLDMTLREIERVLYFEAFVVIEPGMTPMQRGQLL
                     TDEMYLEAIEEHGDEFDARMGAEAVFELLRTIDLNAEIGKVREEMAGTSSETKLKRLS
                     KRLKLVEAFVESHSRPEWMILEVVPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLIELRAPDIIVRNEKRMLQEAVDALFDNGRRGRIITGANKRPLKSLSDMLKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIYSKLEKYGHAT
                     TIKAAKRLVEREGPEVWDILEEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCTAFNADFDADQMAVHVPLSLEAQLEARALMMSSNNILSPANGDPIIVPSQDVVL
                     GLYYLTIEGKNEPGAGMAFADIGEIQAGLNAKAVTLHSPIKARTKHY"
BASE COUNT          286 a          540 c          564 g          341 t
ORIGIN      
        1 catagtgctt ggtgcgagcc ttgatcgggc tgtgcagggt caccgccttg gcgttgagcc
       61 ccgcctggat ctcgccgatg tcggcgaagg ccatccccgc ccccggctcg ttcttcccct
      121 cgatggtgag gtagtagagc ccgagcacca cgtcctggga ggggacgatg atcgggtcac
      181 cgttggcggg cgagaggatg ttgttggacg acatcatgag cgcgcgcgcc tcgagctgag
      241 cctcgagcga cagcggtacg tgtacggcca tttgatcggc gtcgaagtcg gcgttgaagg
      301 cggtacagac gagcgggtgc agttggatcg ccttgccctc gatcagcacc ggttcaaatg
      361 cctgaatgcc aaggcgatgg agcgtcggcg cgcggttcag cagcacggga tgctcgcgga
      421 tcacctcctc gaggatgtcc cacacctccg ggccctcgcg ctccaccagg cgcttcgcgg
      481 ccttgatggt ggtggcgtgc ccgtacttct ccagcttgga gtagatgaaa ggcttgaaca
      541 gctcgagcgc catcttcttc ggcaggccgc attgatggag cttgagctcg ggaccgacga
      601 cgatgaccga gcggccggaa tagtcgacgc gcttgccgag gaggttctgg cggaagcggc
      661 cctgcttgcc cttgagcatg tcggagagcg acttcagcgg ccgcttgttg gcgccggtga
      721 tgatgcggcc gcgccggccg ttgtcgaaga gcgcgtcgac cgcctcctgc agcatgcgct
      781 tctcgttgcg cacgatgatg tcgggcgcac gcagctcgat gagccgcttc aggcggttgt
      841 tgcggttgat gacgcggcgg tagagatcat tgaggtccga ggtggcgaat ctcccgccgt
      901 cgagcggcac cagcgggcgc agctcgggcg ggatcacggg cacgacttcg aggatcatcc
      961 actcgggccg gctatgcgac tcgacaaacg cctcgaccag cttcaggcgc ttcgacaggc
     1021 gcttgagttt cgtctccgag gaggtgccgg ccatctcctc gcgcactttg ccgatctcgg
     1081 cgttgagatc gatggtacgc agcagctcga acacggcctc ggcgcccatg cgggcgtcga
     1141 actcatcgcc gtgctcctcg atcgcctcga ggtacatctc gtcggtgagc agctgcccgc
     1201 gctgcatcgg cgtcatgccc ggctcgatga ccacgaaggc ctcgaaatac agcacccgct
     1261 cgatctcgcg cagcgtcatg tcgagcatca gcccgatgcg cgaaggaagc gacttcagga
     1321 accagatgtg cgcggtgggg ctggccagct cgatgtggcc catgcgctcg cgacgcacct
     1381 tggactgggt cacttccaca ccgcacctcc cgcagatcac gccgcggtgc ttgaggcgct
     1441 tgtacttgcc gcacaggcac tcgtagtcct tcaccgggcc gaagatacgg gcgcagaaca
     1501 ggccgtcccg ctccggcttg aaggtccggt agttgatggt ctccggcttc ttcacttcgc
     1561 cgtgcgacca ctgccggatc ttgtccggcg atgccaatcc gatcttgatc gaatcgaagt
     1621 gctcgaccgg agcgtgctgc ttgaacagtt taagtaagtc tttcatgtct taatcctgct
     1681 ccagttccat gttgatgccg agcgagcgga tctccttcac caggacgttg a
//
LOCUS       DATLKL010000789         1672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_9251, whole genome shotgun sequence.
ACCESSION   DATLKL010000789 DATLKL010000000
VERSION     DATLKL010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9251"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VM713_10710"
     CDS             complement(<1..599)
                     /locus_tag="VM713_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HVH84773.1"
                     /translation="MSAERDAAELEPIERAGEDELRALQLTRLKWSVRHAYENVAPYR
                     NKCQRAGVHPDELRTLADLARFPFTVKDDLRAHYPFGMFAVPRSEVVRLHASSGTTGK
                     PTVVGYTRQDIDTWAGLVARSLRAAGVRPGDIVHIAYGYGLFTGGLGAHYGAERLGCT
                     VVPMSGGQSQKQVQLICDLEPDVIMVTPSYMLALVEEFHR"
     gene            complement(618..>1672)
                     /locus_tag="VM713_10715"
     CDS             complement(618..>1672)
                     /locus_tag="VM713_10715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HVH84774.1"
                     /translation="AAARSSGDSRSAGEAPSGGEAAARSAAAGAGGGARAGEGVPAGS
                     GAGAVAAAPGQLSPAVRRLLAEHGLSLAAVRGSGERGRVTVEDVLRAAAGGAGGAAAR
                     PATEATARPQSATPAEGVRPVPHSAVRRRTAEHMVQSLLHTAPHVTTVFEADLGAVLK
                     DRTRRREEFAQRGAPLTLTAYFVTAAVAAIRAVPEANSRWTDTALEIYDDIHIGIATA
                     VEGTGLVVPVLHDAGARDLFAIARGLEDLVSRARAGKLAPADVRGGTFTISNHGVSGS
                     LLAAPIIINQPQSAILGVGKLEQRPVAVEEGDEQKIVVRPRCYVTLTLDHRVMDGHQA
                     NRFMQAFVGTLADWSA"
BASE COUNT          249 a          616 c          579 g          228 t
ORIGIN      
        1 cggtgaaact cctccaccag cgccagcatg taagaaggag tcaccatgat gacgtcgggc
       61 tcgaggtcgc agatcagctg cacctgcttc tgcgactgcc cgcccgacat cggcacgacg
      121 gtacagccga ggcgctcggc gccgtagtgc gcgccgagac cgccggtgaa gaggccgtag
      181 ccgtaggcga tgtgcacgat gtctccgggg cgcacaccag cggcgcgcag cgagcgggcc
      241 acgagcccgg cccaggtgtc gatgtcctgg cgggtgtagc cgaccactgt cggcttgccg
      301 gtcgtgccgc tcgaggcatg caggcgcacc acctcggagc gcggcacggc gaacattccg
      361 aagggataat gcgcgcgcag atcgtccttc accgtgaagg gaaagcgcgc cagatcggcc
      421 agcgtcctca gctcatccgg atgaacgccg gcccgctgac acttgttgcg atagggcgcg
      481 acgttctcgt aggcatggcg cacggaccac ttgaggcgcg tgagctgcag ggcgcgcagc
      541 tcgtcctcgc cggcgcgctc gatcggctcg agctccgccg catcacgctc cgcggacacg
      601 agcgcccccg cagaacttca ggcggaccag tcggcgagcg tgccgacgaa ggcctgcatg
      661 aagcggttgg cctgatgtcc gtccatcacc cggtgatcga gcgtcagggt gacgtagcag
      721 cgcggccgca ccacgatctt ctgctcgtcc ccctcttcca cggccacggg acgctgctcg
      781 agcttgccga ctccgagaat cgccgactgc ggctggttga tgatgatcgg cgccgccagg
      841 aggctgccgc tcaccccgtg gttcgagatg gtgaaggtgc ccccgcgcac gtcggccggc
      901 gcgagcttgc cggcgcgcgc ccggctgacg agatcctcga gtccgcgcgc gatcgcaaac
      961 agatcgcggg cgccggcgtc gtgcagcacc ggcacgacga gcccggtacc ctcgacggcg
     1021 gtcgcgatgc cgatgtgaat gtcgtcgtag atctcgagcg ccgtatcggt ccagcggctg
     1081 ttggcctcgg gcacggcgcg aatggccgcg accgccgcag tgacgaaata cgccgtgagc
     1141 gtcaaaggcg cgccgcgctg cgcgaactcc tcgcgccggc gcgtacgatc cttcaacacc
     1201 gcgccgagat cggcctcgaa gacggttgtc acgtgcggcg cggtgtgcag caggctctgc
     1261 accatgtgct cggcggtacg ccggcgcacg gcgctgtggg gtacggggcg cacgccctcg
     1321 gcgggagtcg cggactgcgg ccgcgcagtc gcctcggtgg caggccgcgc cgcggcgcca
     1381 ccggcaccgc ctgcggcggc gcgcagcaca tcctccaccg tgacgcgacc gcgctcgccg
     1441 ctaccgcgca cggcagcaag gctcagcccg tgctcggcca gcagccgccg caccgcggga
     1501 ctcaactgcc cgggggcggc cgcgacagct cccgcaccgc taccggcggg cactccctcg
     1561 ccagcccgcg cgccgccgcc agcgccggcg gccgcgctcc ttgccgcagc ctcgccgccg
     1621 gatggcgcct cccccgcact gcgtgagtct cccgatgatc tcgccgcagc gt
//
LOCUS       DATLKL010000790         2710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_178677, whole genome shotgun sequence.
ACCESSION   DATLKL010000790 DATLKL010000000
VERSION     DATLKL010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178677"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VM713_10720"
     CDS             <1..232
                     /locus_tag="VM713_10720"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HVH84775.1"
                     /translation="QGRPEGQIGLTSVRAGDVVGEHTVLFSGPGEQLSLTHRASDREI
                     FARGALAAALWLMSQPPGRYGMRDFIGYKTLA"
     gene            422..1609
                     /gene="carA"
                     /locus_tag="VM713_10725"
     CDS             422..1609
                     /gene="carA"
                     /locus_tag="VM713_10725"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HVH84776.1"
                     /translation="MSIPAVLALADGTIFRGQSIGAKGNTTGEVVFNTALTGYQEILT
                     DPSYARQIVTLTCPHIGNTGTTPEDLEATQIYPAGLIIRDLPRVASNWRSNESLPEFL
                     ERGRVVAIAGIDTRKLTRLLREKGAQAGCIMTGEKADETAAIKLARKFPVLKGMDLAK
                     VVSTKRAFQWNDGTVWPDPARPPIRSQQRLHVVAYDFGIKRNILRLLADFGCRMTVVP
                     AQTKAEEVLALEPDGIFLSNGPGDPEPCTYAIAATRRFLETDMPVFGICLGHQILGLA
                     AGARTVKMKFGHHGANHPVQDLESGRVFITSQNHGFAVDESTLPATVKATHRSLFDGS
                     LQGLSFTDRPAFGFQGHPEASPGPHDMRALFERFNHLMLRRLQAQLKLAEDTLRRQQA
                     GGR"
     gene            1614..>2710
                     /gene="carB"
                     /locus_tag="VM713_10730"
     CDS             1614..>2710
                     /gene="carB"
                     /locus_tag="VM713_10730"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HVH84777.1"
                     /translation="MPKRTDLESILIIGAGPIIIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADAIYIEPINARTVARIIEKERPDALLPTMGGQTALNTALDL
                     VREGVLEKYEIELIAASRAAIDMAEDRELFRNAMREIGLDTPRSQIARSLDEALAIAG
                     EIGYPVVVRPSFTLGGSGGGIGYNREELEDIVARGLDASPTGEVLLEESVIGWKEFEM
                     EVVRDHRDNCIIICSIENLDPMGVHTGDSITVAPALTLTDKEYQRMRDASVAVLRKIG
                     VDTGGSNVQFAVNPRDGRLLIIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LKNDITGGATPASFEPSIDYVVVKIPRFTFE"
BASE COUNT          494 a          910 c          873 g          433 t
ORIGIN      
        1 tcaggggcgc cccgagggac agatcgggct gaccagcgtg cgcgcggggg acgtcgtggg
       61 cgagcacacc gtgctctttt cgggccctgg agagcagctc tccctgaccc atcgggcaag
      121 cgaccgggag atctttgccc gcggagccct ggcggccgcg ctctggctca tgtctcagcc
      181 accgggacgt tacggcatgc gggactttat tggttataaa acattagctt agaccgtcat
      241 cggcgatgtg acttgagctc cagggatgcg ctggggcgag ggaattttcc gtggacacat
      301 cgcccgccaa acccgtaaaa tttctgccta atccgtgtac gggttagtcc atgcaaggcg
      361 ggaggatgtt cgatgagaac tcctcccgct ttgtatatgt gcgtcaaggg gaagtgaaaa
      421 ggtgagcata cctgcggtgc tcgcactggc cgacggaacg atcttccgcg gacagtcgat
      481 cggagccaaa ggcaacacca caggcgaagt cgttttcaac accgccctca cgggttatca
      541 ggaaattctc accgacccgt cctacgcgcg acagatagtc acgctgacct gtccccacat
      601 cggcaacacc ggcacgacgc ccgaggatct cgaggcgacg cagatctatc ccgcgggact
      661 catcatccgc gatctgccgc gcgtcgcgag caactggcgc tcgaacgaat cgctcccgga
      721 gtttctcgag cgcggccggg tggtcgcgat cgccggcatc gacacgcgca agctgacgcg
      781 cctgttgcgc gagaagggcg cgcaggccgg ctgcatcatg acgggggaga aggccgacga
      841 gacggccgcc atcaagctcg cgcgcaagtt cccggtcctg aagggcatgg atctcgccaa
      901 ggtggtgagc accaagcgtg cctttcagtg gaacgacggc accgtctggc ccgacccggc
      961 gcgtccgccg attcgctcgc agcagcggct gcacgtggtg gcctacgact tcggcatcaa
     1021 acgcaacatc ctgcggctcc tcgccgactt cggctgccgc atgacggtgg tgccggcgca
     1081 gaccaaggcc gaggaagtgc tcgcgctcga gcccgacggc atattcctct ccaatggtcc
     1141 cggagacccc gagccctgca cctacgcgat cgcggcgacc cgcaggttcc tcgagaccga
     1201 catgccggtg ttcggcatct gtctcggaca ccagatcctc gggctcgccg ccggcgcgcg
     1261 cacggtgaag atgaagttcg gccatcacgg cgcgaatcac ccggtgcagg atctcgagtc
     1321 gggccgcgtg ttcatcacca gccagaatca cggcttcgcg gtcgatgagt cgactctgcc
     1381 cgcgaccgtg aaggcgacgc atcggtcgct cttcgacggg tcgctccagg ggctcagttt
     1441 caccgaccgt ccggccttcg gtttccaggg ccaccccgag gcgagcccgg gaccgcacga
     1501 catgcgcgcg ctcttcgagc gcttcaatca cctcatgctg cgacgcctgc aggcgcagct
     1561 gaagctcgcc gaggatacgc tgcgccgcca gcaggcgggg gggcgttagg tacgtgccga
     1621 agcgcaccga cctcgagagc atcctgatca tcggcgccgg cccgatcatc atcggccagg
     1681 cgtgcgagtt cgactattcc ggtgcccagg cctgcaaggc gctgcgcgag gagggctacc
     1741 gggtcatcct ggtgaactcg aaccccgcca ccatcatgac ggacccggag atggccgatg
     1801 ccatctacat cgagccgatc aacgcgcgca ccgtggcgcg catcatcgag aaagagcggc
     1861 ccgacgcgct gctgccgacc atgggcggac agaccgcgct caacaccgcg ctcgatctgg
     1921 tgcgcgaggg ggtgctcgag aagtacgaga tcgagctgat cgccgcctcg cgcgccgcca
     1981 tcgacatggc cgaggaccgc gagctgtttc gcaacgcgat gcgcgaaatc ggtcttgaca
     2041 ccccgcgctc gcagatcgcc cgcagcctcg atgaggcgct cgccatcgcc ggcgagatcg
     2101 gctacccggt ggtggtgcgc ccgtcgttca cgctcggcgg cagcggcggc ggcatcggct
     2161 acaaccgcga ggagctcgag gacatcgtgg cgcgcggcct cgacgcctcc ccaaccggcg
     2221 aggtgctgct cgaggagtcg gtgatcggct ggaaagaatt cgagatggag gtggtgcgcg
     2281 accaccgcga caactgcatc atcatctgct cgatcgagaa cctggacccg atgggggtgc
     2341 acaccgggga ctcgatcacg gtcgccccgg ccctcacgct taccgacaag gaataccagc
     2401 gcatgcgcga cgcctcggtc gccgtgctgc gcaagatcgg cgtcgacacc ggcggctcga
     2461 acgtgcagtt cgcggtcaac ccgcgcgacg ggcggctgct catcatcgag atgaacccgc
     2521 gcgtatcgcg ctcctcggcg ctcgcctcca aggcgaccgg cttcccgatc gccaagatcg
     2581 ccgccaagct cgccgtcggc tatacgctcg atgagctgaa gaacgacatc accggcgggg
     2641 cgaccccggc ctcgttcgag ccaagcatcg attacgtggt ggtgaagatc ccgcgtttca
     2701 cgttcgagaa
//
LOCUS       DATLKL010000791         3383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_144978, whole genome shotgun sequence.
ACCESSION   DATLKL010000791 DATLKL010000000
VERSION     DATLKL010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144978"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VM713_10735"
     CDS             <1..327
                     /locus_tag="VM713_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVH84778.1"
                     /translation="GAKGYLLKDAPRQEILSAIRAVSEDRPYTSTTVAAKALQRMAKP
                     SLTQRELDVLQLVAEGRSNKDIARRLSITEGTAKTHVKAILTKLDAISRTEAVAVAHK
                     RGLIHL"
     gene            350..730
                     /locus_tag="VM713_10740"
     CDS             350..730
                     /locus_tag="VM713_10740"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HVH84779.1"
                     /translation="MPLVTLTVRKPKSAKFKTTVLDAVHASLVSSGVPASDRFQRVIE
                     LEEADFRFDPGYPDLQGKRDQDFALIEILWSVGRSVKVKKKFLAELMETLKGAGLNPE
                     NVMVCFKETTWENWSFGGGRLIHT"
     gene            complement(767..1042)
                     /locus_tag="VM713_10745"
     CDS             complement(767..1042)
                     /locus_tag="VM713_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HVH84780.1"
                     /translation="MSRTVQCVLLKREAPGLERPPYPGDLGRRIYESVSREGWALWLK
                     HQTMLINEYRLTPIEPKARKFLEGEMEKFFFGSGSKAPEGYQPPQKS"
     gene            complement(1053..2156)
                     /gene="mutY"
                     /locus_tag="VM713_10750"
     CDS             complement(1053..2156)
                     /gene="mutY"
                     /locus_tag="VM713_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HVH84781.1"
                     /translation="MPLPRASTRSGSPIAAPLLEWHARGGRHDLPWQADRTPYRVWVS
                     EIMLQQTQVAAVRPYFERFVQRFPDVRTLAAAGLDEVLHLWSGLGYYARARNLHRAAG
                     LICSEHGGELPRSFAGLASLPGVGRSTAGAILALSFGERYPILDGNVRRVLARYFGLA
                     GGQGTSDGIRRLWALSERCTPAARVADYTQAIMDLGATVCVRSRPLCAACPLAAGCRA
                     QRTGRQQELPATRSARARRTRSVFMVVALDENGGVLLERRPDSGVWGGLWCLPEFDTV
                     SAARAFIRHSLVTRGIEPQPLSTVEHAFTHFRLVISPLLVRCAGAVADVEEGASVWYN
                     IRAPARIGLPAPITALLAGLARGSMFDAPAARS"
     gene            complement(2213..3124)
                     /locus_tag="VM713_10755"
     CDS             complement(2213..3124)
                     /locus_tag="VM713_10755"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HVH84782.1"
                     /translation="MRMIYRLLPAAAALIASGWAWGGDTSGNFEREVPADAHGVVEIS
                     STNGTIDVTGWDRAAVSVKAELGPGVDRVEVTSSGSHTVVRVIQHSHSAFDWGHDETH
                     LRVQVPKDSEVDVSAVSANVTSAGVLGAQRLKSVSGDITADIGPSDIEVKSVSGTLKL
                     RGKGQAAKLHVSTVSGDLELKHAAGDLEATTVSGEASVELDPARSVRLRSTSGDITFE
                     GRLVRGADFDAQSVSGDLKVRAGSEDGFQFEAATMTGDINDCFHVEAERSSQYGPGHK
                     LDGARGAGSGHMRLKSMSGEIDLCDRK"
     gene            complement(3126..>3383)
                     /locus_tag="VM713_10760"
     CDS             complement(3126..>3383)
                     /locus_tag="VM713_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84783.1"
                     /translation="DPRYQRQRAALLRSLGARLAALPPPARAKVMASLETIRKAKQDL
                     EQALGKGPGNALLQELLVNTYQDEMRVLTDVREASDAGKGI"
BASE COUNT          547 a         1191 c         1130 g          515 t
ORIGIN      
        1 ggcgcgaagg gctacctgct gaaggacgcg ccgcgccagg agatcctctc ggcgatccgc
       61 gccgtgagcg aggaccgtcc ctacacctcg accaccgtgg ccgccaaggc gctgcagcgc
      121 atggcgaagc cgagcctcac acagcgcgag ctcgatgtcc tgcagctcgt ggccgagggt
      181 cgcagcaaca aggacatcgc gcggcggctg tcgatcaccg agggcaccgc caagacccac
      241 gtgaaggcga tcctcaccaa gctcgatgcc atcagccgca cggaagcggt cgcggtcgca
      301 cacaagcgcg gcctgatcca cctgtaggcg ccgccgatcg gaggagttcg tgccgctcgt
      361 gacactgacg gtgcgtaagc cgaagtccgc caagttcaag accaccgtgc tcgatgccgt
      421 gcacgcctcg ctcgtctcgt cgggggtgcc ggcctcggat agatttcagc gcgtgatcga
      481 gctcgaggaa gccgacttcc gcttcgaccc gggatacccg gaccttcagg gcaagcgcga
      541 ccaggacttc gcccttatcg agatactctg gtcggtgggc cgcagtgtaa aagtgaagaa
      601 gaagttcctg gcggagctca tggagaccct caagggcgct ggcctgaacc ctgagaacgt
      661 gatggtctgc ttcaaagaaa cgacctggga gaactggtcg ttcggcggcg ggcgcctgat
      721 ccacacctga acctagggtc gctgcggtcg gtgcggccgc gacgagctag ctcttctgcg
      781 gcggctgata tccctccggt gctttcgatc cagaaccgaa gaagaacttc tccatctcgc
      841 cttcgagaaa tttgcgcgcc ttcggctcga tcggcgtgag gcggtactca ttgatgagca
      901 tggtctggtg cttgagccag agcgcccagc cctcgcgcga cacgctctca taaatgcgcc
      961 ggccgagatc gccgggatag ggcggccgct cgaggcccgg cgcctcgcgc ttcaagagaa
     1021 cacactgcac cgttcgtgac atggagaccc cgctaggaac gcgctgccgg tgcatcgaac
     1081 atcgagccgc gcgccaggcc agcgagcagc gcggtgatgg gtgcgggcag gccgatgcgc
     1141 gccggcgcac ggatgttata ccagacactc gcaccctcct ccacgtccgc gaccgcgccc
     1201 gcacagcgca ccagcaatgg gctgatgacg aggcggaagt gcgtaaaggc atgctcgacg
     1261 gtgctcaagg gttgcggctc gatcccgcgc gtcaccagag agtggcggat gaacgcccgc
     1321 gcggcggaga cggtatcgaa ctccggcagg caccagagcc cgccccacac cccgctgtcc
     1381 ggccgccgct cgagcagcac gccgccgttc tcatcgagcg ccacgaccat gaacaccgag
     1441 cgggtgcgcc gcgcgcgtgc ggagcgtgtg gcggggagct cctgctggcg acccgttcgc
     1501 tgcgcgcggc agccggccgc gagcggacag gccgcgcaca gcggccggga gcgcacacac
     1561 accgtggcgc cgagatccat gatcgcctgc gtgtagtcgg ccacgcgtgc ggccggcgtg
     1621 cagcgctcgg acagcgccca cagccgcctg atcccgtcgc tcgtgccctg gccacccgcc
     1681 aggccaaagt agcgcgccag gacccgccgc acgttgccgt cgaggatcgg atagcgctca
     1741 ccaaagctga gcgcgaggat cgccccggcg gtcgagcggc cgacgcccgg caggctcgcg
     1801 agcccggcga agctgcgcgg cagctccccg ccgtgctcgc tgcagatcag tcccgctgcg
     1861 cggtgcaggt tgcgcgcccg cgcgtagtag ccgagcccgg accacagatg cagcacctcg
     1921 tcgaggcccg ctgcggcgag cgtgcgcacg tcggggaagc gctgcacaaa gcgctcgaag
     1981 tacggcctga cggccgcaac ctgggtctgc tgcagcatga tctccgacac ccacacccga
     2041 tagggagtgc ggtcggcttg ccaggggagg tcgtgtcgcc cgccgcgggc gtgccactcg
     2101 agaagtggcg cggcgatggg agatccgcta cgggtcgatg ctcggggcag gggcacgcgg
     2161 aggattctaa cgcccccgcg ctcatctcag ccgccgcagc gcccgtggcc gttcatttgc
     2221 gatcgcacag gtcgatctcg ccgctcatgc tcttcaggcg catgtgcccg ctgcccgcac
     2281 cgcgcgcgcc gtcgagcttg tggccgggcc catattgact gctgcgctcg gcttcgacgt
     2341 ggaagcagtc gttgatgtcg ccggtcatgg tcgcggcctc gaactggaag ccatcctcgg
     2401 agccggcgcg caccttgagg tcgccactca ccgactgcgc atcgaaatcc gcgccgcgca
     2461 ccaggcgtcc ctcgaaggtg atgtcaccgg aagtgctgcg cagccgtacc gagcgcgccg
     2521 gatcgagctc gaccgaggct tccccgctca ccgtcgtcgc ctcgagatcg ccggcggcgt
     2581 gtttgagctc gagatcgccg ctcacggtgc tgacgtgcag cttcgccgct tgtcccttgc
     2641 cgcggagctt gagggtcccg ctcaccgact tcacttcgat gtccgacggt ccgatgtcgg
     2701 cggtgatgtc accgctcacc gacttcaggc gctgggcgcc gagcacgccc gcggaggtca
     2761 cattcgcact cacggccgag acgtcaacct cactgtcctt cggcacctgc acgcgcaggt
     2821 gcgtctcgtc gtgcccccag tcgaaggcgg agtgtgagtg ctggatcacg cgcacgacgg
     2881 tgtgactgcc cgagctcgtc acctctacgc gatcgacgcc gggaccgagc tcggccttga
     2941 cgctcacggc cgcgcggtcc cagccggtca cgtcgatcgt gccgttggtg ctcgagatct
     3001 ccacgacccc gtgggcgtcc gccggtacct cgcgctcgaa gttcccggaa gtgtcgccgc
     3061 cccacgccca gccgctcgcg atgagcgcag cagcggcggg cagcaaccga tagatcatcc
     3121 gcatgtcaga tccccttgcc agcgtcgctg gcttcacgca cgtcggtgag gacgcgcatc
     3181 tcatcctggt aagtgttcac gagcagctcc tgcagcagcg cgttgcccgg gcccttgccg
     3241 agcgcctgct ccaggtcctg cttggccttg cgaatggtct cgaggctcgc catgactttg
     3301 gcgcgcgccg gcggcggcag cgccgcgagc cgcgcgccga gcgagcgcag cagcgccgcg
     3361 cgctggcgct ggtagcgcgg atc
//
LOCUS       DATLKL010000792         1590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_32083, whole genome shotgun sequence.
ACCESSION   DATLKL010000792 DATLKL010000000
VERSION     DATLKL010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32083"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1590)
                     /locus_tag="VM713_10765"
     CDS             complement(<1..>1590)
                     /locus_tag="VM713_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84784.1"
                     /translation="AFGIYDHNLVCACETLQADGAPAPEAAVPRERLWKIRARAVIAA
                     TGAFERPLVFPDNDRPGVMLAGAAEKYARAFGVACGQRAVLAAACDNAYAIAAGLRAA
                     GVEIVALIDAREASPASEAARDFRVVTGGRVAAVSGTRGVRGLRIALGRGSRSLALDC
                     DLLLNLGGYAPAVHLHSQAGGKLRWLEEESMFVPDGPAPGVASVGACAGAFVRAAALG
                     HAAEVGEALARGRAAPPAPRGGVGRCDPGPGPRPRGRCFVDLQNDVTADDVALAVREN
                     YRSVEHLKRYTTTGMGTDQGKTSNVNALALLGELTARSAPEVGTTRFRPPFAPVTLGA
                     IAGRRVGSLYRPLKRLPAEAWHVAHGALLENFGDWCRPAAYPQPGESLEAAAQREARA
                     VRGSAGLLDGSPLGKLEVFGPDAGRFLDLMYVGTMSTLAPGQARYGVLLNENGTLVDD
                     GIVARLGPEYFWINTTSAGAERTAAAFEEWQQCEFTDLEVRLTPVTSRWANVTVAGPR
                     AWEWLARAGFDAGLAPPAMRH"
BASE COUNT          232 a          612 c          547 g          199 t
ORIGIN      
        1 ggtgtcgcat ggccggcggc gcgagccccg catcgaagcc ggcccgcgcg agccactccc
       61 aggcgcgcgg acccgcgacc gtaacgttgg cccagcgcga cgtcaccggt gtcagccgca
      121 cttcgaggtc cgtgaactcg cactgctgcc actcctcgaa ggccgccgcg gtgcgctccg
      181 ccccggcgct cgtcgtattg atccagaaat actcgggccc gagccgcgcg acgatcccgt
      241 cgtcgaccag cgtgccgttc tcgttgagca acacgccgta gcgggcctgg cctggggcga
      301 gcgtcgacat ggttcccacg tacatgaggt cgagaaagcg ccccgcgtcc gggccgaaga
      361 cctcgagctt gccgagcggc gagccgtcga ggagcccggc gctcccgcgc actgcgcgcg
      421 cctcgcgctg cgccgctgcc tcgagcgatt cgcccggctg cggataggcc gccggccggc
      481 accagtcgcc gaagttctcg agcagcgccc cgtgtgccac gtgccaggcc tccgccggca
      541 accgcttcag cggccggtac aaagagccca cccgccggcc tgcgatcgct cccagcgtca
      601 ccggcgcgaa cggcggccga aagcgcgtgg tgccgacctc gggagctgag cgcgcggtga
      661 gctcgccgag cagcgcgagc gcgttgacgt tgctcgtctt cccctggtcg gtccccatgc
      721 cggtcgtggt gtagcgcttc agatgctcga ccgagcggta gttctcccgg accgcgaggg
      781 cgacatcgtc tgctgtcaca tcgttctgca gatcgacgaa gcagcggcca cgtgggcgcg
      841 gtcctgggcc cggatcacaa cgcccgacgc caccgcgcgg cgccggcggc gcggcccgac
      901 cgcgcgcgag cgcctcgccc acctcggctg cgtggccgag cgccgcggcg cgcacgaagg
      961 cgccggcgca ggcgccgacg ctcgccacac ccggtgcggg gccgtccggc acgaacatcg
     1021 actcctcctc cagccagcgg agcttcccac ccgcctggga atgcagatgc accgccgggg
     1081 catagccgcc caggttgaga aggagatcgc agtcgagcgc gaggctgcgc gagccgcgcc
     1141 cgagcgcaat tctcaggccg cgcacgccgc gtgtgcccga gacggcggca acgcgtccac
     1201 cggtcacgac acgaaaatcg cgcgccgcct cgctcgccgg cgaggcctcc cgcgcatcga
     1261 tgagcgccac gatctcgaca ccggcggccc gaagacccgc ggcgatcgcg taggcgttgt
     1321 cgcaggcggc ggcgagcacc gcacgctgcc cgcaagccac gccgaaggcg cgcgcgtatt
     1381 tctcggcggc gccggccagc atcactcccg gccgatcgtt gtcggggaac acgagcggcc
     1441 gctcgaacgc accggtcgca gcgatcaccg cgcgtgcgcg gatcttccat aaccgttcgc
     1501 gcggcaccgc agcttccggc gccggcgcgc cgtcggcctg cagcgtctcg caggcgcaga
     1561 cgaggttatg gtcgtagatc ccgaaggcca
//
LOCUS       DATLKL010000793         2161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_54615, whole genome shotgun sequence.
ACCESSION   DATLKL010000793 DATLKL010000000
VERSION     DATLKL010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54615"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /gene="atpG"
                     /locus_tag="VM713_10770"
     CDS             complement(<1..693)
                     /gene="atpG"
                     /locus_tag="VM713_10770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HVH84785.1"
                     /translation="MPGTKEIRAKISSVKSTQKITKAMQMVATSKMRRAQERMKLARP
                     YAKRMLEVIGHLTEANPDYRHPFLVTREPKGIGLIVISSDRGLAGALNANVFRETLKL
                     IREWQAKGAAVSLCLIGTKALNFFRRLSLPILANVTHLGDRPRVKDLIGTVKVMLDGY
                     RAGELDRVFLINAQFVNTMTQKPVVQQLLPIEALDTEGLQEHWDYIYEPDAAGILDGL
                     LMRYIESQVYRAA"
     gene            complement(698..>2161)
                     /gene="atpA"
                     /locus_tag="VM713_10775"
     CDS             complement(698..>2161)
                     /gene="atpA"
                     /locus_tag="VM713_10775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004723422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HVH84786.1"
                     /translation="NVGTIVSVTDGITRIHGLADARYGEMLEFPGNTFGLALNLEQDS
                     VGAVVLGEYQHLGEGDTVKTTNRILEVPVGPGLLGRVVDALGAPLDGKGPVKAARTAP
                     IERVAPGVIYRKSVSQPVQTGYKAIDSMVPIGRGQRELIIGDRQTGKTALAVDTIINQ
                     KGSGVKCIYVAIGQKQSTIANVVRKVEEHGALAHTVVVAASASTPAALQYIAAYSGVT
                     MGEYFMDNGEDALIIYDDLSKQAVAYRQISLLLRRPPGREAFPGDVFYLHSRLLERSA
                     RVNEEYVTMVSKGAVKGKTGSLTGLPIIETQAGDVTAFVPTNVISITDGQIFLETDLF
                     NAGIRPAMNAGISVSRVGGAAQTDIIKKLGGGIRLALAQYRELAAFSQFASDLDEATR
                     RQLERGQRVTEVMKQKQYAPMSVAQMALSIYAVNNGYMDKVELKKVVAFEAALQAFAE
                     SNYQPMLDAINANPKLSKESEAAMKKCIEDFVATGTY"
BASE COUNT          328 a          697 c          730 g          406 t
ORIGIN      
        1 cgcggcgcga tagacctggg actcgatgta gcgcatgagg agcccgtcga ggatgcccgc
       61 ggcatccggc tcgtagatgt agtcccagtg ctcctgcaac ccctccgtgt cgagcgcttc
      121 gatcggcagc agctgctgca cgacgggctt ctgcgtcatg gtgttgacga actgcgcgtt
      181 gatgagaaag acgcgatcga gctcgcccgc gcggtagccg tccagcatca ccttgacggt
      241 gccgatgaga tccttgacgc gcggccggtc gccgagatgc gtgacgttgg cgagaatcgg
      301 gagagagagg cgccggaaga agttgagcgc cttggtgccg atcaggcaca ggctcaccgc
      361 cgcgcccttg gcctgccatt cacggatcag cttcaaggtc tcgcggaaca cgttggcgtt
      421 cagcgccccc gccaggccgc gatccgagga gatcacgatg aggccgatcc ccttcggctc
      481 gcgcgtgacg aggaacgggt ggcggtagtc ggggttggcc tcggtgaggt ggccgatcac
      541 ttccagcatg cgcttggcgt aggggcgcgc cagcttcatg cgctcctgcg cccggcgcat
      601 cttgctggtc gccaccatct gcatcgcctt ggtgatcttc tgagtgctct tgacactcga
      661 gatcttggcg cggatttcct tggtgccggg catgtgccta gtaggtcccg gtcgccacga
      721 agtcctcgat gcacttcttc atcgccgcct cgctctcctt ggagagcttg ggattggcat
      781 tgatggcatc gagcatcggc tggtagttgc tctccgcgaa cgcctgtagc gccgcctcga
      841 aggccaccac cttcttcagc tccaccttgt ccatgtagcc attgttgacc gcgtagatgg
      901 agagcgccat ctgcgccacc gacatgggcg cgtactgctt ctgcttcatg acctcggtga
      961 cgcgctggcc gcgctcgagc tggcggcggg tcgcctcgtc gagatcggag gcgaactgtg
     1021 agaaggcggc gagctcgcgg tactgggcga gtgccaggcg gataccgccg ccgagcttct
     1081 tgatgatgtc ggtctgcgcg gcgccgccga cgcgcgacac cgagatgccg gcgttcatcg
     1141 ccgggcggat gccggcgttg aagagatcgg tctcgaggaa gatctgcccg tcggtgatcg
     1201 agatgacgtt ggtcggcacg aacgccgtca catctcccgc ctgcgtctcg atgatcggca
     1261 gtcccgtgag tgagccggtc ttgcccttga ccgcgccctt gctgaccatg gttacgtatt
     1321 cctcgttgac gcgcgccgag cgctcgagga gccgcgagtg aagatagaac acgtcgcccg
     1381 gaaaggcctc gcgcccgggc gggcggcgca gcagcagcga gatctgccgg taggcgaccg
     1441 cctgcttcga cagatcgtca taaataatga gtgcgtcctc gccgttgtcc atgaagtact
     1501 cgcccatggt gacgcccgag tacgcggcga tgtactgcag ggcggccggg gtggaggcgg
     1561 aggcggccac caccacggtg tgggcgagcg cgccgtgctc ctcgaccttg cgcaccacgt
     1621 tggcgatcgt cgactgcttc tggccgatcg cgacgtagat gcacttaacg ccggagccct
     1681 tctggttgat gatggtgtcg accgccagcg cggtcttgcc cgtctgccgg tcgccgatga
     1741 tgagctcgcg ctggccgcgg ccgatcggca ccatcgagtc gatggccttg tagccggtct
     1801 gcaccggctg gctcaccgac ttcctgtaga tgacgcccgg cgccacgcgc tcgatcggcg
     1861 cggtgcgcgc cgccttcacc gggcccttgc cgtcgagcgg cgcaccgagc gcatcgacca
     1921 cgcgcccgag gagccccggc cccaccggca cctcgaggat gcggttggtg gtcttgacgg
     1981 tgtccccttc ccccaggtgc tggtactcgc cgagcaccac ggcgccgacc gagtcctgct
     2041 cgaggttgag cgcaagcccg aaggtgttgc cggggaattc cagcatttcg ccgtagcgcg
     2101 catcggcgag accgtggatg cgggtgatgc cgtcggtcac cgagacgatc gtgccgacgt
     2161 t
//
LOCUS       DATLKL010000794         2302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_178771, whole genome shotgun sequence.
ACCESSION   DATLKL010000794 DATLKL010000000
VERSION     DATLKL010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178771"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VM713_10780"
     CDS             complement(<1..593)
                     /locus_tag="VM713_10780"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVH84787.1"
                     /translation="MRIIAVTNIKGGVGKTTTAVNLAYLCACAGHATLIWDLDPQGAA
                     TYGLRGEAHERASAKKLLSGKRELPELVLATGYPGLDLLPADFSYRNFSVHLAERRHP
                     TERLLRMSRPLREIYHTLFLDCPSGISLLTENVLRAADGVIVPLVPSPLSVRVLSQLC
                     DFLVREDWSDLTLLPFFSMVDRRRSLHQDLMAGTRAQ"
     gene            complement(606..1061)
                     /locus_tag="VM713_10785"
     CDS             complement(606..1061)
                     /locus_tag="VM713_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HVH84788.1"
                     /translation="MASELSKADVPKVVETLNRILELELAGLVRYLHYSFMIFGHNRI
                     PIVAWLRTQADESQAHAVAAGEHITNLGGHPSLRIGQLLETHKHNVDEILREALAHEE
                     EGLGEYRKLLEQVSGRSIMLEEYARIQIAAEEAHLAEIGKMVRKPGSIT"
     gene            1130..1609
                     /locus_tag="VM713_10790"
     CDS             1130..1609
                     /locus_tag="VM713_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HVH84789.1"
                     /translation="MGGFYDIAVRRIDGTLDLLGALRGNVTLAVNVASQCGLTPQYAG
                     LEQLHRELGAERFTVVGFPCNQFGAQEPGSEAEIIAFCRTSYDVSFPLSAKLEVNGAN
                     RHPLYAFLTSPETGIAGDISWNFEKFLISRDGRVLKRYPPETRPEDRGLMQDIAEAL"
     gene            1606..>2302
                     /locus_tag="VM713_10795"
     CDS             1606..>2302
                     /locus_tag="VM713_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HVH84790.1"
                     /translation="MSEFDFAILGAGAMGSIVGAHLARAGHRVAMLARGTRADALEMH
                     GLTIRGLVEFTTPVHTVRNPAALTSARALIVATKTPGTAEALAALRHAEFAVTLSIQN
                     GPLKNELLAQAFGAPRVLGALADTSGELLPGGEVLFTRNVNIMIGELDGTESARARDL
                     AHTIDAAGVRAAATREIVTLEWSKFCGWVGLMALSVTTRAETWKYLSDPDAALVLVRL
                     VRDVGTLAHALRIP"
BASE COUNT          378 a          783 c          780 g          361 t
ORIGIN      
        1 aactgcgcac gggtgcccgc catcaggtcc tggtggagcg agcggcgccg atcgaccatc
       61 gaaaaaaacg gcaggagcgt gagatcggac cagtcctcgc gcacgagaaa atcgcacagc
      121 tgactcagca cccgcaccga cagcggcgag ggtaccagcg gcacgatcac gccgtcggcg
      181 gcccgcagca cgttctcggt gaggagtgaa atgcccgacg gacaatcgag aaacagcgtg
      241 tggtagatct cacgcaacgg ccggctcatg cgcagcagcc gctcggtcgg atgacggcgc
      301 tcggcgagat gtaccgagaa gttgcgatag gaaaaatccg ccggcagcag atcgagcccg
      361 gggtaaccgg tggccagcac cagctccggg agctcgcgct tgcccgagag gagcttcttc
      421 gccgaggccc gctcgtgcgc ctcaccccgc aggccgtaag ttgctgcgcc ctgcggatcg
      481 agatcccaga tgagcgtcgc atgcccggcg cacgcgcaca ggtaggcgag gttcaccgcg
      541 gtggtggtct tgcccacacc gcccttgatg ttggtgacgg cgatgatccg cacggcgcgt
      601 cggcttcagg tgatcgagcc cggcttgcgc accatcttgc cgatctcggc cagatgcgcc
      661 tcctcggcgg cgatctggat gcgcgcgtac tcctcgagca tgatgctgcg gccactcacc
      721 tgctcgagaa gcttgcgata ctccccgagt ccctcctcct cgtgtgccag cgcctcgcgc
      781 aggatctcat cgacattgtg cttgtgagtc tcgagcagtt ggccgatgcg gagcgagggg
      841 tggcccccga ggttggtgat gtgctcgccg gcggccacgg cgtgcgcctg cgactcatcg
      901 gcctgagtgc gcagccaggc cacgatcggg atgcggttgt gcccgaagat catgaagctg
      961 tagtgcaggt agcgcaccag tcccgcgagc tcgagctcga ggatccgatt gagggtctcg
     1021 accaccttgg gcacgtcggc cttgctgagc tcggaagcca tgaggatctc cggcgggggc
     1081 gctgcgcgca tgttaagccc gcgggcgggg tatggtaaaa agcgccggta tgggcggttt
     1141 ctacgacatc gcggtgcgcc gcatcgacgg cacgctggac ctcctcgggg cgctgcgcgg
     1201 caacgtaacg ctcgccgtca acgtggcgag ccagtgcggc ctgacgcccc agtacgccgg
     1261 gctcgagcag ctgcatcggg agctcggcgc cgaacgcttc acggtggtcg gcttcccctg
     1321 caaccagttc ggcgcccagg agccggggag cgaggcggag atcatcgcct tctgccgcac
     1381 gagctacgac gtcagcttcc cgctcagcgc caagctcgaa gtgaacggcg ccaaccggca
     1441 tccgctctac gccttcctca cctcgcccga aaccggcata gctggcgaca tcagctggaa
     1501 tttcgagaag ttcctgatct cgcgcgacgg tagggtgctc aagcgctatc cgcccgagac
     1561 caggcccgag gaccgcggtc tcatgcagga catcgccgag gcgttgtgag cgagttcgat
     1621 ttcgcgatcc tcggcgccgg cgccatgggc tcgatcgtcg gggcgcatct cgcccgcgct
     1681 ggacaccgcg tcgcgatgct ggcgcgcggg acgcgcgccg acgcgctcga gatgcacggc
     1741 ctcaccatcc gcgggctggt ggagttcacg actcccgtcc atacggtgcg caatcccgcg
     1801 gcgctcacga gcgcgcgggc gctcatcgtc gcgaccaaga cgccgggcac ggcggaggcg
     1861 ctcgctgcgc tcagacatgc cgagttcgcc gtcacgctct cgatccagaa cgggccgctg
     1921 aagaacgagc tgctcgcgca ggccttcggc gcgccccgag tgctcggtgc tctcgccgac
     1981 acgagcggcg agctgctgcc cggcggcgag gtgctattca cccgcaacgt gaacatcatg
     2041 atcggcgagc tcgacggtac cgagagcgcc cgcgcccgcg acctcgcgca cacgatcgac
     2101 gccgccgggg tgcgcgccgc ggcgacccgc gagatcgtga cgctcgagtg gtcgaagttc
     2161 tgcggctggg tggggctcat ggccctctcg gtcacgaccc gagccgagac ctggaagtac
     2221 ctctccgatc ccgacgccgc gctggtgctg gtgcggctgg tgcgtgacgt gggaactctc
     2281 gcgcacgccc tccgcatccc ct
//
LOCUS       DATLKL010000795         1542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_88553, whole genome shotgun sequence.
ACCESSION   DATLKL010000795 DATLKL010000000
VERSION     DATLKL010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88553"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1542
                     /gene="bamA"
                     /locus_tag="VM713_10800"
     CDS             <1..>1542
                     /gene="bamA"
                     /locus_tag="VM713_10800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HVH84791.1"
                     /translation="PNWLSWYKQDDRYSRESLQGDLEKVRNFYMDRGYANFQLESTQV
                     AIAPEKEDIFITINIDEGQVYKISSIKLAGTFVVPQAELERLLLVHPGEIFDRKLITS
                     SQELMQNRMGRDGYAFAKVEPVPTADNSNHTVSLTFFVDPGNRVYVRNITFSGVNRIN
                     DEVLRRELRQLEGGWLSNVALERSKQRIQRLPYVKTVEFETTPVAGVPDQVDVNYKIE
                     EGPASQLSGGIGYSATYKFQLNASFADADFLGSGERVAVELNGGAFSKVYTLAWTNPY
                     TTIDNLQRTLSVSYRDVTQFVSSSSNFSSQTLTAGPTWAYPISETMYLRFGAVFESSQ
                     LLTNSLTSAEQAQQWVRENGHPYSRIAHDDASNNEYVFYGSNFKNVQAVLGWDWDTRN
                     RTLFADRGMHQAISVSATTPGSDVRYWIGNYQFLRYLPLWKGFTLSFLEGVDYGAPLA
                     STTSIPPYRQFFGGGPDNVRGFRESLLGPRDQFGNPYGGNLRVTSQNELILPMPAKWA
                     QTARVA"
BASE COUNT          285 a          545 c          459 g          253 t
ORIGIN      
        1 gcccaactgg ctctcctggt acaagcagga cgaccgttat tcgcgcgagt cgctgcaggg
       61 cgacctggag aaggtgcgca acttctacat ggaccgcggc tacgccaact tccagctcga
      121 gtcgactcag gtagcgattg cgccggagaa ggaagacatc ttcatcacca tcaacatcga
      181 cgagggccag gtctacaaga tctcctcgat caagctggcc ggcaccttcg tcgtgccgca
      241 ggccgagctc gagcggctgc tgctggtgca cccgggcgag atcttcgacc gcaagctcat
      301 cacctcttcc caggagctga tgcagaaccg catggggcgc gacggttacg cctttgccaa
      361 ggtcgagccg gtgccgaccg ccgacaacag caaccacacc gtgtcgctca ccttcttcgt
      421 cgaccccggc aaccgcgtgt acgtgcgcaa catcaccttc tcgggcgtca accgcatcaa
      481 cgacgaggtg ctgcggcgcg agctgcgcca gctcgagggc ggctggctgt cgaacgtggc
      541 gctcgagcgc tccaagcagc gcatccagcg cctgccgtac gtgaagacgg tggagttcga
      601 gaccacgccg gtggcgggcg tccctgatca ggtggacgtg aactacaaga tcgaggaggg
      661 ccccgcttcg cagctgtcgg gcggcatcgg ctactccgcg acctacaagt tccagctcaa
      721 cgccagtttt gccgacgccg acttcctcgg ctcgggcgag cgcgtcgcgg tcgagctgaa
      781 cggcggcgcc ttcagcaagg tatacacgct ggcgtggacc aatccctaca ccaccatcga
      841 caacctgcag cgcaccctct cggtgagcta ccgcgacgtg acgcagttcg tctcctcgtc
      901 gtcgaatttc tcctcgcaga ccctcaccgc cggccccacc tgggcttacc cgatcagcga
      961 gaccatgtat ctgcgcttcg gcgccgtgtt cgaatcctcg cagctcctca ccaactccct
     1021 caccagcgcc gagcaggcgc agcagtgggt gcgggagaac ggccacccct acagccgcat
     1081 cgcgcacgat gacgcgagca acaacgaata tgtcttctac ggctcgaact tcaagaacgt
     1141 gcaggcggta ctcggctggg attgggacac gcgcaaccgc acactgttcg ctgaccgtgg
     1201 catgcatcag gcgatcagcg tctcggcgac caccccgggg agcgacgtgc gctactggat
     1261 cggcaactac cagttcctgc gctacctgcc gctgtggaag ggattcacgc tgtcgttcct
     1321 cgagggcgtc gactacggcg caccgctcgc cagcaccacc tccatcccgc cctaccgcca
     1381 gttcttcggc ggcgggccgg acaacgtgcg cggcttccgc gagagcctgc tcggcccgcg
     1441 cgatcagttc ggcaacccct acggcggcaa cctgcgcgtc accagccaga acgagctgat
     1501 cctgccgatg ccggccaagt gggcgcagac cgcgcgcgtc gc
//
LOCUS       DATLKL010000796         2998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_20726, whole genome shotgun sequence.
ACCESSION   DATLKL010000796 DATLKL010000000
VERSION     DATLKL010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20726"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /locus_tag="VM713_10805"
     CDS             complement(<1..1438)
                     /locus_tag="VM713_10805"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HVH84792.1"
                     /translation="MDRRTMMIGTAFAAAAAAARALPQGTDSSKQLNALFDQFMKENL
                     DISPTFTTSLGMDTGERARQKSEIDDGSEAGIERQKALTASQLARLKALDRAGLSAAD
                     GSSYDVVMYGLRTNDDANRAFHYGAGGAGQPYVLSQLTGSYQQLPSFLDNQHTIESKA
                     DADAYVARLAGFARLLDQETEVARHDSGLGVAPPDFALAKALTQMRDLRAPAPEKSPL
                     TDSVVRRAKEKNISGDYAQQASRVVQQQIYPALDRQIAVISEMQRNATHDAGVWRLPD
                     GEAYYAASLVIWATTGKQPDEIHRLGLELVKDHAARIDGLMRKIGMTEGTVGERLRAM
                     YADAKFQYANTDAAKEGLIADLNARVQRVRAKLPQYFGALPKANVEIKRVPKEIEAGA
                     PGGSYNSPSLDGKRPGIYWINLRNTSEQPKWTLPTLTYHESIPGHHLQLSIQREANLP
                     LIRRVSFYSAYIEGWALYAEQLAGEMGEY"
     gene            complement(1583..2248)
                     /locus_tag="VM713_10810"
     CDS             complement(1583..2248)
                     /locus_tag="VM713_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVH84793.1"
                     /translation="MIRVLIADDQTLIRQGIRTLLEMDPGVSVVGEAADGDETVQRVR
                     SGPVDVLLLDIRMPGKDGIAVLRALSADNILPPTLILTTFDDSDVVLDGIRAGARGFL
                     LKDVSYPQLLAAIRAVAEGATVFQPAVTERLLRAGSALRLSAEPPREALTAREAGVVQ
                     LMAGGYSNREIAGALGTAEGTIKNHVSNILAKFGVRDRTRAVLKALETGLLRPPGPST
                     SGR"
     gene            complement(2245..>2998)
                     /locus_tag="VM713_10815"
     CDS             complement(2245..>2998)
                     /locus_tag="VM713_10815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVH84794.1"
                     /translation="TFTLQGAWLLLPPYLALQLVAFFVFRAMALEVAARTALAGSNAE
                     LRGVAQILSDSSRIAERLRIARELHDALGHHLTALSLNLEAALQRTEGAANASVATAQ
                     GLARRLLGDVREIVAESRTRDGINVAEALGTLAEAMPRPRIHLDIPAELRTPDPEHAH
                     TLLRCAQEIVTNAARHSNAENLWLTVRCEGETLRIEARDDGRGSTTTDGGFGIRGMRE
                     RIAKAGGELRVATEPGRGFEVTAYLPVRGGAA"
BASE COUNT          444 a          992 c         1021 g          541 t
ORIGIN      
        1 cgtattcgcc catctcgccc gccagctgtt cggcgtacag cgcccagcct tcgatatacg
       61 cgctgtaaaa tgacacccgg cggatgaggg gcagattcgc ttcccgctga atggacagct
      121 gcagatggtg tccgggaatg ctctcgtggt aggtcagcgt cggcagcgtc cacttgggct
      181 gctcgctcgt gttgcgcagg ttgatccaat aaatgcccgg ccgcttgccg tcgagagagg
      241 gactgttgta agagccgccg ggggcgccgg cttcgatttc cttcggcacc cgtttgatct
      301 cgacattggc tttgggcagc gcaccgaagt actgcggcag cttcgcccgc actcgttgca
      361 cgcgcgcgtt gagatccgcg attagcccct ccttggcggc atccgtattc gcgtactgga
      421 atttcgcgtc cgcgtacatg gcgcgcagcc gctcgccgac cgtgccctct gtcatgccga
      481 tcttgcgcat gaggccatct atgcgcgccg catggtcctt caccagctcg agtcccagcc
      541 ggtggatttc gtcaggctgc ttaccggtcg tggcccagat gacgagcgat gcggcgtaat
      601 aggcctcacc gtcaggcagg cgccacacgc cggcatcgtg ggtcgcgttt ctctgcattt
      661 cgctgatcac ggctatctga cgatcgagcg ccgggtagat ctgctgctgc accacgcggc
      721 tcgcctgctg cgcgtagtcg ccggagatgt tcttctcctt ggcacggcgc acgactgagt
      781 cggtgagcgg cgacttctcg ggcgccggcg cacgcaggtc ccgcatctgc gttagcgctt
      841 tggcaagcgc gaagtccggt ggcgcaactc cgagacctga gtcgtgccgc gcaacctctg
      901 tttcctggtc gagcagtcgc gcaaaacccg ccagacgcgc gacgtacgca tccgcgtcgg
      961 ccttgctctc gatggtgtgc tggttgtcga gaaacgacgg cagctgctgg tagctgccgg
     1021 tgagctggct gagaacatat ggctggccgg cgccgccggc gccgtagtga aaggcgcggt
     1081 tggcatcgtc attggtgcgc agtccgtaca tgacgacgtc gtagctgctg ccatcggcgg
     1141 cgctgaggcc ggcgcggtca agggccttga ggcgggcgag ctggctcgca gtcagcgcct
     1201 tctgccgttc gattcctgct tccgacccat cgtcgatctc gctcttctgc ctggcgcgct
     1261 cgccggtgtc catgccgagg ctggtcgtga aggtcgggga gatgtcgaga ttctccttca
     1321 tgaactgatc gaacagggcg ttcagctgct tcgagctgtc ggtgccctgg ggcagcgcgc
     1381 gcgcggcggc cgcggcggcg gcgaaggcgg tgccaatcat catggtgcgg cggtccatga
     1441 gaccctcttc agattctttg tggcgtcagg ggcttaagcc tgtcacaact cgccccagcg
     1501 cgcggcagcc gttgaggcga ggcgatggta tagaacgttg aggcgttgcg ccgcgacgct
     1561 gcggggcagt tgtagccgcg cgtcagcggc ctgacgtgga aggacccggg ggccgcagca
     1621 gccccgtctc cagcgccttt agtacggcgc gcgtccggtc ccggacaccg aacttggcga
     1681 gaatgttgga gacgtgattc ttgatggtgc cctcggcagt cccgagggcc cctgcgatct
     1741 cgcggttact gtagccgcca gccatcagct gcacgacccc ggcttcgcgg gcggtgagcg
     1801 cctcacgagg tggctcagcc gagaggcgga gcgcgctccc ggcgcgcagc agccgctcgg
     1861 tcaccgccgg ctgaaacacc gtggcgcctt cggcgaccgc ccggatggct gcgagcagtt
     1921 gcgggtagga gacgtccttc agcaggaacc cgcgagcgcc agcccggata ccatcgagca
     1981 ccacgtcgct gtcgtcgaag gttgtcagga taagagtcgg cggcaggatg ttgtccgccg
     2041 acagcgcgcg caacaccgcg atgccgtcct tgccgggcat gcggatatcg aggagcagca
     2101 catcgaccgg ccccgaccgc acgcgctgca ccgtctcgtc gccgtccgcg gcttcgccga
     2161 ctaccgaaac gccagggtcc atctccagca gcgtgcggat gccctgccgg atgagcgtct
     2221 ggtcatcggc gattagaact cggatcacgc agcgccgccg cgcaccggca ggtaggctgt
     2281 cacctcgaag ccccgcccgg gctcggtcgc gacccgcagc tctccgcccg ccttggcaat
     2341 gcgctcgcgc atgccgcgga ttccgaagcc gccgtccgtg gtagtgctgc cgcggccgtc
     2401 gtcgcgcgcc tcgatgcgca acgtctcgcc ctcgcagcgg acggtcagcc acaggttctc
     2461 ggcatttgag tgtcgtgcgg cattggtgac gatctcctgg gcgcagcgca gcagcgtgtg
     2521 cgcgtgctcg ggatcggggg tgcggagctc cgcggggata tccagatgga tgcgcggcct
     2581 gggcatcgcc tcggccagag ttccgagtgc ctcggcgacg ttgattccgt cccgggtgcg
     2641 cgactcggcc acgatctcgc gcacatcgcc gagcagtctc cgcgccaaac cctgcgcggt
     2701 cgcgacgctg gcgttcgctg cgccctcggt gcgctgcagc gccgcctcga ggttgagcga
     2761 cagcgcggtg aggtgatggc ccagtgcatc gtgcagctcg cgggcgatcc gcagccgctc
     2821 ggcgattcgg ctgctgtcgg acagaatctg agccaccccg cgcagctccg cattgctgcc
     2881 cgcgagtgcc gtccgggcgg caacctcgag cgccatggcc cggaacacga agaacgcgac
     2941 gagctgcagc gcgaggtacg gcggcagcag cagccaggca ccctggagag tgaaggtg
//
LOCUS       DATLKL010000797         2334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_223468, whole genome shotgun sequence.
ACCESSION   DATLKL010000797 DATLKL010000000
VERSION     DATLKL010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223468"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VM713_10820"
     CDS             <1..714
                     /locus_tag="VM713_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HVH84795.1"
                     /translation="EKLAYPLADHVGQPTNPALGLRAIRLSLRSQELIEAQLAAILRA
                     GAHGPVRILLPMISSVAEVRQVRAILKRVHARLKRQGVKLADSPPPVGVMIEIPGAAL
                     AADALAMESDFFAIGTNDLTMYTLAIDRSDEQVAYLYNPLHPAVLRLIQFAAGAALRA
                     RLPLCLCGEMAGDPRYTALLLGLGIRDLSMPVNALPRIKRRIRALALSEATRRAELIM
                     NQWDTGRIAALLDDFDALA"
     gene            complement(743..1303)
                     /locus_tag="VM713_10825"
     CDS             complement(743..1303)
                     /locus_tag="VM713_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84796.1"
                     /translation="MIGIRGGRLKPLARWGAGAIAAIVAGMLWSHSASPALTEAAKAT
                     AEGLGPVRIGMTVVQAEKEIGAQIVYDTTAGTEESCRYAWPNAGMKQVRFIISHGVIV
                     RIDVNDYEITTEKGARIGDSEARIKSLYRGDVVEMPHPYIAGGHDLIVPSADNKYAIL
                     FETDGEVVLGYRMGDRKAVGWSSGCQ"
     gene            1540..>2334
                     /locus_tag="VM713_10830"
     CDS             1540..>2334
                     /locus_tag="VM713_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014872381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HVH84797.1"
                     /translation="MAKTSGKTAKEDYRVADIGLADWGRREIAIAETEMPGLMAVRAE
                     YGRSQPLKDARIAGSLHMTIQTAVLIDTLKALGADVRWASCNIFSTQDHAAAAVAASD
                     TPVSAYKGESLAEYWEFTHRVFEWADGGAPNMILDDGGDATLLVHLGLSAETNPAVLA
                     NPANEEEQALYAAIKRRLDAKPGWYAAIAKAIRGVTEETTTGVHRLYQMAKEGRLLFP
                     AINVNDSVTKSKFDNLYGCRESLVDGIRRATDVMMAGKVAMVCGFGD"
BASE COUNT          399 a          821 c          725 g          389 t
ORIGIN      
        1 gaaaaactcg cctatccgct cgccgatcat gtcggccagc cgaccaatcc cgcgctcggc
       61 ctgcgggcga tccgcctctc gctcaggagc caggagctga tcgaggccca gctcgccgcc
      121 atactacgcg caggcgccca cggccccgtg cgcattctcc tgccaatgat cagctcggtc
      181 gccgaggtgc gccaggtgcg ggcgatcctc aagcgggttc acgcgcggct caagcggcag
      241 ggggtcaagc tcgccgattc gccgccgccg gtcggagtca tgatcgaaat ccccggcgcg
      301 gcgctggccg ccgacgcgct cgcgatggag agcgacttct tcgcgatcgg caccaatgac
      361 ctcaccatgt acaccctcgc gatcgaccgc agcgacgagc aggtcgctta tctctacaac
      421 cccctccacc ccgccgtgct tcgcttgatc caattcgccg ctggggcggc cctccgcgcc
      481 cgcctaccgc tctgcctttg cggcgagatg gcgggggatc cgcgctacac ggcgctgctg
      541 ttgggacttg gcattcgcga tctctcgatg ccggtgaacg cgctgccgcg catcaagcgg
      601 cgcatccgcg cgctcgccct cagcgaggcg acgcgccgcg ccgagctcat catgaaccag
      661 tgggatacgg gccgcatcgc agccctgctc gacgatttcg acgcgctggc gtgagcgaag
      721 gcgcgccgca gcgcttgccg ccctactggc agcccgatga ccagcccacc gccttgcggt
      781 cacccatccg atagccgagc acgacctcgc cgtcggtctc gaacaggatg gcatacttgt
      841 tgtcggcgct tggcacgatc agatcgtgcc cgccggcaat gtaggggtgc ggcatctcca
      901 ccacatcgcc gcggtatagg gatttgatgc gcgcttccga atcgccgatc cgcgccccct
      961 tctcagtggt gatctcgtaa tcgttgacat cgatgcgcac gatgacgccg tgggagataa
     1021 tgaatcggac ctgcttcatg ccggcgttcg gccacgcgta gcggcacgac tcctcggtgc
     1081 cggcggtcgt atcgtagacg atctgcgccc cgatttcctt ctccgcctgg accaccgtca
     1141 tgccgatacg gacgggcccg aggccctccg ccgtcgcctt ggcggcctcg gtcagcgccg
     1201 ggctcgccga gtgcgaccag agcatgcccg caacgatcgc agcgatcgca ccggcccccc
     1261 aacgcgcgag cggcttcagc cgtccgcccc gaattccgat catggtccac atacctccac
     1321 tcgggccgca tcctagtaag gatgataagg tctttccaca gcggaaccca agccggcgtg
     1381 ctcgtcgggg tcggcgtccc ggcgcggagc aaacataacg atataaacat ttctttatat
     1441 ttccccttgc ccgggcgccg cgtcgctgct ataggaccgc cgtcccccgc agtgccgcct
     1501 gctccggggg ccgtcgatcg tttccgcgag gagaatccga tggcgaagac atctggcaag
     1561 actgccaagg aagactaccg ggtcgccgac attggcttgg ccgattgggg gcgccgcgag
     1621 atcgcgattg cggaaacgga gatgccgggg ttgatggcgg tgcgggcaga atatggccgg
     1681 agtcagccgc tcaaggacgc cagaatcgcc gggtcgctcc acatgacgat ccaaaccgcg
     1741 gttcttatcg acacgctcaa ggcactgggc gccgacgtcc gctgggcctc gtgcaacatc
     1801 ttctcgacgc aggaccatgc cgccgccgcc gtcgccgcct ccgatacgcc ggtctccgcc
     1861 tacaagggcg aaagcctcgc cgaatactgg gaattcaccc accgagtctt cgagtgggcg
     1921 gatggcgggg cgcccaacat gatcctcgac gacggcggcg atgcgacgct cttggtccat
     1981 ctcggcctca gcgccgagac gaatcccgca gtgctcgcca atccggccaa cgaggaggag
     2041 caggcgctct atgccgccat caaacgcagg ctcgacgcga agccgggctg gtacgcggcc
     2101 atcgccaagg ccatccgcgg cgtgaccgag gagacgacga ccggtgtcca ccgcctttac
     2161 cagatggcga aggaggggcg gctgctgttc ccggccatca atgtcaacga ctcggtcacc
     2221 aagtcgaagt tcgacaacct ctatggctgc cgcgagtcgc tcgtggacgg tattcgccgc
     2281 gccaccgacg tgatgatggc aggcaaggtc gcgatggtct gcggcttcgg cgac
//
LOCUS       DATLKL010000798         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_156677, whole genome shotgun sequence.
ACCESSION   DATLKL010000798 DATLKL010000000
VERSION     DATLKL010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156677"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VM713_10835"
     CDS             complement(<1..582)
                     /locus_tag="VM713_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVH84798.1"
                     /translation="MDLLGTLRGILGAGDLLENDACAPFESDHRRLYEGRALAVALPR
                     SVDQVSRILAACNAARVGVVPHGGNTSYCGGATPDESGTQLVLSLARLNRVRSVDPEN
                     YSLIAEAGCVLARVQQAAEAAERHFPLSLGSEGSCQIGGNLSTNAGGVHVLRYGMMRD
                     LVLGLEVVLADGRVLPGLTALRKDNTGYDIRGLF"
     gene            complement(602..1492)
                     /gene="htpX"
                     /locus_tag="VM713_10840"
     CDS             complement(602..1492)
                     /gene="htpX"
                     /locus_tag="VM713_10840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HVH84799.1"
                     /translation="MKRILLFIVTNLAVLLVLTVVARVLGIDAYLAAHGQSLQGLLVW
                     AALFGFGGAFVSLALSKMQAKMFMGVQVIGPQSGDASEQWLLSVIEHHARNVGVGMPE
                     VGIFDSPEPNAFATGMSRNSALVAVSTGLLQRMNRQEIEAVLGHEMTHVANGDMVTLA
                     LIQGIVNTFVLFLARVVGNIVDRAVFRSDDGRGIASYLTFYVLQIAFGILATPIVMWF
                     SRWREFRADRGGAQLAGTGNMIAALEELKRVHEPLPAQQFAAFGIADGAVGSGLRRLF
                     MSHPPLDERIAALRALGPTN"
     gene            complement(1595..>2530)
                     /locus_tag="VM713_10845"
     CDS             complement(1595..>2530)
                     /locus_tag="VM713_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HVH84800.1"
                     /translation="LAAEEQASRWEQAAARDSGTSVERVFTPSMRARVVARLLDTFDP
                     ASLRPVLAAMKLRGLSIYSALSGAIAGHAMPTSLAEVGARHRSTLGGSLRAAVFGVND
                     GLVSNLSLVLGVAGAGVARGYVLMSGAAGLLAGALSMAAGEYVSVRSQREMYEYQIAL
                     EREELKEYPEEEAEELALIYQARGVELEQARAVSHALLAHPDKALDVLAREELGLNPD
                     DLGFPWRAASVSFVTFAGGALVPLVPFLAGVAGSAALALSAAMAAVALFAVGLSLSLF
                     TGRDALRSALRMVLIGGGAGTLSFLVGRALGVALS"
BASE COUNT          424 a          896 c          883 g          327 t
ORIGIN      
        1 gaagagtccg cggatgtcat aaccggtgtt gtccttgcgc agcgccgtga gaccggggag
       61 cacgcggccg tcggccagca ccacctcgag cccaagcacc aggtcgcgca tcatgccgta
      121 gcgcagtacg tgcacgccgc cggcgttcgt cgagagattg ccgccgatct ggcagctgcc
      181 ctcggagccg agactcaggg ggaagtgccg ctcggccgcc tccgcagcct gctgcacccg
      241 ggcgagcacg cagcccgcct ccgcgatcag cgagtagttc tcggggtcaa cgctgcggac
      301 gcgattgagc cgggcgagcg acagcaccag ctgcgtaccg gactcatcag gggtcgcccc
      361 gccgcagtag ctggtgttgc caccatgcgg caccacgccg acgcgcgcgg cgttgcaggc
      421 ggcgagaatg cgcgagacct gatcgaccga gcgcggcagc gccacggcca gggcgcgtcc
      481 ctcgtagagg cggcgatggt cactctcgaa gggcgcgcag gcgtcgttct cgagcagatc
      541 cccggcgccg agaatcccgc gcagcgtccc gagaaggtcc accgcttcga aggcggtcgc
      601 gtcagttcgt ggggccgagc gcgcgcagcg ccgcgatgcg ctcatcgagc ggcgggtggc
      661 tcatgaagag gcgccgcagt cccgagccga ccgcgccatc ggcgatgccg aaggcggcaa
      721 actgctgtgc gggcagcggc tcgtgcaccc gcttcagctc ctcgagcgcg gcgatcatgt
      781 tgccggtgcc ggcgagctgc gcgccgccgc ggtcggcgcg gaactcgcgc cagcgcgaga
      841 accacatgac gatcggcgtg gcgaggatgc cgaaggcgat ctgcaggacg taaaaggtga
      901 gatacgaggc gatgccccgg ccgtcgtcgg agcggaacac cgcccggtcg acgatgttgc
      961 cgacgacccg cgccaggaac agcacgaagg tgttgacgat gccctgaatc agggcgagcg
     1021 tcaccatgtc gccgttggcg acgtgcgtca tctcgtgacc gagcaccgcc tcgatctcct
     1081 gccggttcat gcgctggagc agcccggtgc tcacggccac gagcgccgag ttgcggctca
     1141 tgccggtggc gaaggcgttc ggctcgggag agtcgaagat gccgacctcc ggcatgccga
     1201 ccccgacgtt gcgggcgtga tgctcgatca ccgacagcag ccactgctcg ctcgcatcgc
     1261 ccgactgcgg gccgatcacc tgcaccccca tgaacatctt cgcctgcatc ttcgacagcg
     1321 ccagcgagac gaaggcgccg ccgaagccga acagcgccgc ccataccagc aatccctgca
     1381 ggctctggcc gtgagcggcc agataggcgt cgatccccag cacccgtgcg accacggtca
     1441 gcaccaggag cacggccagg ttggtcacga tgaacaggag gatgcgtttc atggcaggtc
     1501 tgcggcgtaa aacggggttg gcactgtacc gttacccctc actgctcgcc agccttgggg
     1561 ctagggccca catttcaagg ccggccgcgc gccttcagga gagggccact cccagcgcac
     1621 gaccgaccag gaagctcaga gttcccgcac cgccgccgat cagcaccatg cgcagcgcac
     1681 tgcgcagcgc gtcgcgtccg gtaaagagcg agaggctgag cccgacggca aagagcgcga
     1741 ccgcggccat ggccgcggac agggccagcg ccgcggaccc ggctacgccc gcgaggaacg
     1801 gcacgagcgg cacgagggcg ccgcccgcga acgtgacgaa tgagacgctc gccgcgcgcc
     1861 acgggaagcc gagatcgtcg ggattcaggc cgagctcctc gcgcgcgagc acatcgagcg
     1921 ccttgtccgg gtgcgcgagc agcgcgtggc tcacggcacg cgcctgctcg agctcgacgc
     1981 cgcgcgcctg gtagatgagg gcgagctcct cggcttcctc ctcggggtac tccttgagtt
     2041 cctcgcgctc gagcgcgatc tggtactcgt acatctcgcg ctgcgagcgc accgacacat
     2101 actcgcccgc cgccatggac agcgcgccgg ccagcagccc cgccgctccc gacatcagca
     2161 cgtagccgcg cgcgacgccg gcacccgcca ctcccagcac caggctcagg ttcgacacga
     2221 gtccatcgtt cacgccgaag accgcggcgc gcaggctccc gccgagggtg ctgcggtggc
     2281 gcgccccgac ttccgcgagc gaggtgggca tggcgtggcc cgcgatggca ccggagagcg
     2341 cgctgtagat ggagaggccg cgcagcttca tcgccgccag caccggacgg agcgatgccg
     2401 ggtcgaaggt gtcgagcagg cgcgccacga cgcgggcgcg catcgagggg gtgaagacgc
     2461 gctctacgga ggtgccggag tccctggcgg cggcctgctc ccagcggctg gcctgctcct
     2521 cggcggcgag
//
LOCUS       DATLKL010000799         1868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_54702, whole genome shotgun sequence.
ACCESSION   DATLKL010000799 DATLKL010000000
VERSION     DATLKL010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54702"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..>1868
                     /locus_tag="VM713_10850"
     CDS             231..>1868
                     /locus_tag="VM713_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVH84801.1"
                     /translation="MRTPVSLASLTLAALALGATAPAPAQSPEASDWGYYGGDMFGQR
                     YSSLDQINRANVRQLTVAWTYRTGELGAGFAQARRLTFEATPVLAFGRLYLETGTNIV
                     IALDPVSGHEQWRFDPRVDRARRYAESTSRGVSVWANTDPRWTGPCAHRVFTGTIDAR
                     LLAIDADTGKPCADFGSGGQVDLTRGIRIRDRPAYLVTSPPAIYANVVIVGSSIGDNR
                     AADVERGVIRGFDALSGALIWSFDPLPDSPTHPAAADWSLTQAASTGAGNAWGVMTVD
                     EEHALVLVPTGSASPDFYGGTRLGRNRFADSLLALDARTGKLVWHQQLVHHDLGDYDL
                     AAQPALGDLDLQGAPVAAVIEATKTGMLYVFDRTNGAALFPVTERPVPRSLVPGERAS
                     PTQPFSALPALVPQRRVEPADAWGLTFWDRGKCREKLAALRNEGIFTPPDTRGTLEFP
                     GYAGGVNWGGVVFDEQRGRVIAAVNDMPMEVTLIPSAELEAQQRSGLYPHAEFGRQAG
                     TPYAMRRVPLLSPWGLPCTAPPWGTLVSVDLHANRIVW"
BASE COUNT          263 a          686 c          652 g          267 t
ORIGIN      
        1 agagcgtgag cggctgccgc gaacgcgagg cactgagctg cgagagagaa cctcaatgcc
       61 ccgtaccggg acatgaacgc ccgtggtgaa ctcacggcgc gaagaatacc agcagcgggc
      121 gggcgacgcg ctgcggctcg cgctccggcg ccggcctcgc ggataatgcc gtcatgaccc
      181 gagccgacgg cagcgcgcgc ttgcgaatcc gctcacgcga cggggtgcct gtgcgcaccc
      241 ccgtctcgct cgcctcactg actctcgcgg ctcttgccct cggcgcaacc gcgccggcgc
      301 cggcacagtc tcccgaggcg agcgactggg gctactacgg cggggacatg ttcggtcagc
      361 gctactcgag cctcgaccag atcaaccgcg ccaacgtccg ccagctcacc gtggcctgga
      421 cctaccgcac cggtgagctc ggcgcgggct tcgcgcaggc gcgacgcctg accttcgagg
      481 cgacgccggt gctcgccttc ggacggctgt acctcgagac gggcaccaac atcgtgatcg
      541 cgctcgatcc cgtgagcgga cacgagcagt ggcgctttga cccgcgcgtc gaccgcgcac
      601 gccgctacgc ggagagcacc tcgcgcggcg tcagtgtctg ggcgaacacc gatccgcgct
      661 ggaccgggcc gtgcgcgcac cgggtattca ccggaaccat cgacgcgcgg ctcctggcga
      721 tcgatgctga caccggcaag ccgtgcgcgg atttcggcag cggcggccag gtggacctga
      781 cgcgcggcat ccgcatccgc gaccgtcccg cctacctcgt gacttcgccc ccggcgatct
      841 acgccaacgt ggtgatcgtc ggctcctcga ttggcgacaa ccgcgccgcg gacgtcgagc
      901 gcggcgtgat ccgcggcttc gatgcgctga gcggcgctct catctggtcg ttcgatccgc
      961 tgcccgactc gccgacgcat cccgccgccg ccgattggag cctcacccag gccgccagca
     1021 ccggcgccgg caacgcgtgg ggcgtgatga cggtggacga agagcacgcg ctggtgctgg
     1081 tgccgaccgg ctcggcgagt cccgatttct acggcggcac gcgcctgggc cgcaaccggt
     1141 tcgccgactc gctgctcgcc ctcgacgcgc gcaccggcaa gctcgtgtgg catcagcagc
     1201 tcgtgcacca cgacctgggg gattacgacc tcgcggcgca gccggcgctc ggggatctcg
     1261 acctgcaggg cgcgccggtg gcggccgtga tcgaggcgac caagaccgga atgttgtatg
     1321 tgtttgaccg caccaacggg gcggccctct tcccggtgac cgagcgtccg gtgccgcgca
     1381 gcctggtgcc gggcgagcgg gcctcgccga cccagccgtt ctcggcgctg ccggcgctcg
     1441 tcccgcagcg gcgcgtcgag cctgccgacg cctggggcct caccttctgg gaccgcggca
     1501 agtgccgcga gaagctcgcc gcgctgcgca acgagggcat cttcaccccg cccgacacgc
     1561 gcggcacgct ggagtttccc ggctacgcgg gcggcgtgaa ctggggcggc gtggtcttcg
     1621 acgagcagcg cgggcgggtg atcgccgcgg tcaacgacat gccgatggag gtgacactga
     1681 ttccgtcggc ggagctcgag gcgcagcagc gctccgggct ctacccgcac gccgaattcg
     1741 gccgccaggc gggcactccc tatgccatgc ggcgcgtgcc gctgctctct ccgtggggac
     1801 tgccgtgcac ggccccgccc tggggcacgc tggtgagcgt ggatctgcac gccaaccgga
     1861 tcgtctgg
//
LOCUS       DATLKL010000800         1503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_122522, whole genome shotgun sequence.
ACCESSION   DATLKL010000800 DATLKL010000000
VERSION     DATLKL010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122522"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VM713_10855"
     CDS             complement(<1..279)
                     /locus_tag="VM713_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003171120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HVH84802.1"
                     /translation="MFHVVLFQPEIPPNTGNVIRLCANTGATLHLVRPLGFELNRKAV
                     RRAGLDYEELAEVRVHASLAACLEALDTARLFSVETGGARAYSEATFSA"
     gene            317..1336
                     /locus_tag="VM713_10860"
     CDS             317..1336
                     /locus_tag="VM713_10860"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HVH84803.1"
                     /translation="MRVMPSATPEAAGAHAPPDAAATALRFSFCGLDFTSPIVLLSGC
                     VGFGEEYTRVEGFSNRDAGAIVLKGTTGKPRLGNPPHRIYETPAGMLNAIGLQNPGVE
                     YVVSKVLPQLDFTETRFIANVCGSTVEEYVEVTRRFDESPIDAMEINISCPNVREGGV
                     AFGNYPEMSAQVVAACRRVTKKPLITKLSPNQTDIRENARRCIEAGTDALAVINTVMG
                     MAIDVATRRPVIGNVQGGLSGPAIKPIALLKVHQVYEVAGPHKVPIIGQGGIVSATDA
                     LEFIIAGASAVGVGTALFYDPLVCRSINDGIAEYLRAQGLKNVTQLVGTLRTGRDIQD
                     CAVSG"
BASE COUNT          246 a          505 c          532 g          220 t
ORIGIN      
        1 cgcgctgaag gtcgcctcgc tgtaggcccg cgcgccgccg gtctcgacgc tgaagaggcg
       61 cgcggtatcc aaggcctcga ggcaggcggc gaggcttgcg tgcacgcgca cctcggcgag
      121 ctcctcgtag tcgaggccgg cgcggcgcac ggccttgcga ttgagctcga acccgagcgg
      181 gcgcacgagg tgcagcgtcg ctccggtgtt cgcacacaga cgaatcacgt tgccggtatt
      241 cggcgggatc tcgggctgga acagcacgac gtggaacatt ttgggctcgc gtagcggggt
      301 ggcgcgctgt gcgataatgc gcgtcatgcc gagcgcgaca ccagaagcag ccggggcgca
      361 cgccccgccg gacgcggccg ccaccgcgct gcgcttctcc ttctgcggcc tcgacttcac
      421 ctcgcccatc gtgctgctgt cgggctgcgt cggcttcggc gaggagtaca cgcgggtcga
      481 gggcttctcg aaccgcgacg cgggcgccat cgtcctcaag ggcacgaccg gcaagccccg
      541 cctcggcaac ccgccacacc ggatctacga gacgccggcc ggcatgttga acgccatcgg
      601 cctgcagaac cccggggtcg agtacgtggt gagcaaggtg ctgccgcagc tcgacttcac
      661 cgagacgcgc ttcatcgcca acgtgtgtgg ctcgacggtg gaggaatacg tggaggtcac
      721 gcggcgcttc gatgagtcgc cgatcgacgc catggagatc aacatctcct gcccgaatgt
      781 cagagagggc ggggtcgcct tcggcaacta cccggagatg tcggcgcagg tggtcgccgc
      841 ctgccgccgc gtgaccaaga agcccctcat caccaagctc tcgcccaacc agaccgacat
      901 ccgcgagaac gcgcgccgct gcatcgaggc ggggacggat gcgctcgcgg tgatcaatac
      961 cgtcatgggc atggcgatcg acgtcgcgac gcgccgcccg gtgatcggca acgtgcaggg
     1021 cgggctctcg gggccggcca tcaagccgat cgcgctcctg aaggtgcacc aggtctacga
     1081 ggtggcgggc ccccacaagg tgccgatcat cggccagggc ggcatcgtca gcgcgaccga
     1141 tgcgctcgag ttcatcatcg ccggcgcgag cgcggtcgga gtcggcacgg cgctcttcta
     1201 cgacccgctg gtgtgccgga gcatcaatga cggaatcgcg gagtacctgc gcgcgcaggg
     1261 gctgaagaac gtgacccagc tggtcggtac cctgcgcacg ggccgcgaca tccaggactg
     1321 cgcggtctcg ggttgacggg ggaggaggga gcatgaggag aacgacaaca ctttcgctca
     1381 aggcgctcgc ctggggactc gccgcggcgc tcgcgggctg cgtgatcgag gccccctatc
     1441 agtccgcgcc tgtcgacact tcggcggccc cggtcccggc gaccgatgag ctcgaggagg
     1501 agg
//
LOCUS       DATLKL010000801         4306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_111119, whole genome shotgun sequence.
ACCESSION   DATLKL010000801 DATLKL010000000
VERSION     DATLKL010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111119"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VM713_10865"
     CDS             <1..255
                     /locus_tag="VM713_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84804.1"
                     /translation="RGPVESPWRAPLALFAAAAPAGLLGELELACLSAALSASAAAGL
                     SGRLFLNVSPHSLLRRSIGLASSRCSAVVTPSSRQAVCSS"
     gene            243..1736
                     /locus_tag="VM713_10870"
     CDS             243..1736
                     /locus_tag="VM713_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVH84805.1"
                     /translation="MLELRDRSLLEDFERIHTTLRALRARGFDFAIDEFGTGHSGLRM
                     WAQLRPNFVKIDPYFAHHVERDPVKLEFVRSLMDMGRAVGSRVIVAGVESQEACGELL
                     DLEVDGLQGYLFGRPEAALSGTQQALAPLREPRAAAQPTTTAGDLAVEVAPVHPSMHV
                     SAFVRLVQDRSHCAAFPVVDEAGVPVGMIWRDRFLVMYSRPLHPEVLARKPVSAIMDS
                     TPLIVDARLRLEQTSRLVTRKSRIHGTDQFIIVRDGRYAGVGHTIDLLAHITQSRIQA
                     ATHCNPLTTLPGNVPIHDNLERFIQQDRPFVVGYADLDNFKPFNDHYGYAKGDQVLLQ
                     LAQVLAGSIARRTDFVGHIGGDDFVVILRSPDWALRLARVIEEFAARVNGLYSTQDLA
                     RGGIEAFGRYGERRTFRLLSLSIAVLDSRTRRFSSAEDVSEHLQAAKRRAKAVEGNCL
                     WYEGPQGGRNLLEDPEFVTQSAASPPPQLEIDLDTGTFELESPIAQI"
     gene            complement(1757..2728)
                     /locus_tag="VM713_10875"
     CDS             complement(1757..2728)
                     /locus_tag="VM713_10875"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HVH84806.1"
                     /translation="MDAEPQGDPVYGRVFNTSAVPGVQAEGVVSIHYREDEGHYYPDG
                     FQWRMRVPHYQLSGLRHGPLARTTVIKPRLGPSLLGVGWLEAVPEVAIVGAGAPGTGL
                     PAWDVYQGVRTLGRFGWQGGAVSIRDQTTKALAREMGLTSSDRPHDDCTTFEVDCLRQ
                     PDGGTPEVADETVAALVSFVRTLTVPDSPAHSMRGSGGAEIFARVGCADCHQPQLPVE
                     RHEAEGEHTRGVITPYTDLRLHDLGAEMADEDASGARVPSRWRTAPLWGLGYRVNSGA
                     TLLHDGRARTPEEAILWHAGEAARARRNFVNLLHRQREALLQWLDSL"
     gene            complement(3226..3537)
                     /gene="rpsJ"
                     /locus_tag="VM713_10880"
     CDS             complement(3226..3537)
                     /gene="rpsJ"
                     /locus_tag="VM713_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HVH84807.1"
                     /translation="MANQNIRIRLKAFDHRLIDSSAKEIVETAKRTGATVKGPVPLPT
                     KMERFTVLISPHADKDARDQFELRTHKRLMDIMNPTDKTVDALMKLELPAGVDVQIKL
                     N"
     gene            complement(3550..>4306)
                     /locus_tag="VM713_10885"
     CDS             complement(3550..>4306)
                     /locus_tag="VM713_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HVH84808.1"
                     /translation="LLELVEMEVRELLTTYKFPGDATPIVIGSALKALEGDKGELGVP
                     SVVKLVEEMDSYIPIPKREIDKPFLMPVEDVFSISGRGTVVTGRIERGIVRVNDEVEI
                     VGLRPTAKTVCTGVEMFRKLLDEGQAGDNVGVLLRGTKREEVERGQVLAKPGSITPHT
                     HFEGEVYVLTKEEGGRHTPFFKGYRPQFYFRTTDVTGAIELPAGAEMVMPGDNIKMTI
                     ELISPITMDQGLRFAIREGGKTVGAGVVVKILK"
BASE COUNT          664 a         1533 c         1362 g          747 t
ORIGIN      
        1 cggggaccgg tggagagccc ctggcgagcg ccgctcgcgc tgttcgcggc cgcagcccca
       61 gccggcctcc tcggggagct cgagctcgcc tgtctgtcgg cagccctgag cgccagcgcg
      121 gcggcaggtc tcagcgggcg gctgttcctc aatgtctcac cgcactcgct gctgcgccga
      181 tcgattggcc tggcgagctc gagatgctct gccgttgtca cgccgtcgag ccggcaagct
      241 gtgtgctcga gctgagggac cgatcgctgc tcgaggactt cgagcgcatt cacaccacgc
      301 tgcgggccct gcgcgcgcgc ggcttcgact ttgcgatcga tgagttcggt accggccatt
      361 cgggcctgcg tatgtgggcc cagctgcgcc ccaatttcgt gaagatcgac ccctatttcg
      421 cccatcacgt cgagcgcgat ccggtgaagc tggagttcgt acgctcgctc atggacatgg
      481 ggagggcggt cggcagccgt gtgatcgtcg cgggagtcga gagccaggag gcatgtggcg
      541 agctgctcga tctcgaggtc gacggcctgc aagggtatct gttcgggcgc cccgaggcgg
      601 cgctctcagg cacgcagcag gcactcgccc ccctgcgcga gccgcgcgcc gcggctcagc
      661 ccaccaccac cgcgggcgac ctcgccgtcg aggtcgcgcc cgtgcacccc tcgatgcacg
      721 tgagtgcctt cgtgcggctg gtgcaggacc gctcgcattg cgccgccttc ccggtggtgg
      781 acgaggccgg cgtgccggtc ggcatgatct ggcgcgaccg cttcctggtc atgtactcga
      841 ggcccctgca tccggaggtg ctcgccagga agccggtgag cgccatcatg gactcgacgc
      901 ccctcatcgt cgacgcgcgg ctgcgtctcg agcagaccag ccgcctcgtg acgcgcaagt
      961 cgcgcattca cggtaccgat cagttcatca tcgtgcgcga cggccgttat gccggtgtgg
     1021 gccacacgat cgatctgctc gctcacatca cgcagagccg catccaggcg gcgactcact
     1081 gcaatccgct caccaccctg ccgggcaacg tgccgatcca cgacaacctc gagcgcttca
     1141 ttcaacagga ccgccccttc gtggtcggct acgccgatct cgacaacttc aagcccttca
     1201 atgaccacta cggctacgcc aagggcgatc aggtgctgct gcagctcgcg caggttctgg
     1261 cgggctcgat cgcgcggcgg accgatttcg tcggtcacat cggcggcgat gatttcgtgg
     1321 tcatcctgcg ctcgcccgac tgggcgctgc ggctcgcccg cgtcatcgag gagttcgcgg
     1381 cgcgcgtcaa cggtttgtac tcgactcagg acctggcacg gggcggcatc gaggcgttcg
     1441 gccgctatgg cgagcgccgc acgttccggc tgctctcgct ttcgattgcc gtcctcgaca
     1501 gccgcacccg tcggttctcg agcgccgagg atgtgtcgga gcatctgcag gccgccaagc
     1561 gtcgcgccaa ggcggtcgag ggcaattgcc tctggtatga gggcccgcag ggcgggcgaa
     1621 acctgctcga ggatccggag ttcgtcaccc agtcggccgc atccccgccg ccccagctcg
     1681 agatcgacct cgataccggg accttcgagc tcgagagtcc catcgcgcag atctagcgtc
     1741 tgacgcaagc gtaggatcac agggaatcaa gccactgcaa cagagcctct cgctgccggt
     1801 gcagcagatt cacgaagttg cgccgcgcgc gggctgcctc cccggcatgc cagagaatcg
     1861 cctcttcggg agttcgtgcc cgaccgtcgt gcagcagggt cgcgccgctg ttgacccggt
     1921 aacctagccc ccagagcggc gcggtccgcc acctgctcgg aactcgcgcc ccgctcgcgt
     1981 cctcgtcggc catttcggcg ccgaggtcgt ggagccgcag gtcggtgtag ggagtgatga
     2041 ctcctcgcgt gtgctcgccc tcggcctcgt ggcgctcaac gggcaactgg ggctgatggc
     2101 agtcggcgca gccgaccctc gcaaaaatct ccgcaccgcc cgaaccccgc atcgagtgcg
     2161 ccggggagtc cggcaccgtg agcgtgcgca cgaagctgac gagggcggcg actgtctcgt
     2221 cagcgacctc cggcgtgccg ccatcgggct gtcgcaggca atccacctcg aacgtcgtgc
     2281 agtcatcgtg cgggcgatcg ctggacgtca gccccatttc ccgcgccagc gccttggtcg
     2341 tctggtcgcg gatcgacacc gcgccgccct gccagccgaa ccgaccgagt gtccgcacgc
     2401 cctggtatac atcccaggcg ggcaatcccg tgcccggggc accggcgccg acaatagcca
     2461 cttcgggaac cgcttcgagc cagccgacgc cgagcagcga cggccccagg cgcggtttga
     2521 tgacggtggt gcgcgcgagg ggtccgtgtc tcaggccgct cagctggtaa tgcggtaccc
     2581 gcatgcgcca ttggaagccg tcagggtagt agtgcccttc gtcttcacga tagtggatcg
     2641 agacgacgcc ttcggcctgg acgccaggta cggccgaggt gttgaagacg cgaccgtaca
     2701 ccggatcgcc ttgcggctcg gcatccacgc tggccgccgg cgtttcgagt tgaacgacga
     2761 gcgcggcggg cgccggccca tctcccgatg gacccttgcc accctcacca ctgctgccat
     2821 ggcacgccgt gcacgagtcg gcgttgaaaa gcggtcccag cccctgccca actccggcgg
     2881 tgccggcggg cacccaatgc cggctgaaaa cggccctgcc gacgctgacg cgtgcagctt
     2941 cgtccgctcc cgcggcgccg gcgctgacgg cacaaagggc tgcagcagtc agccaccccg
     3001 agcctctcgc ccgagtggcg cggattgacc tgcggccggc gggactagtg cgatgcatgg
     3061 tgcctaccct gccgcgtcaa gaccccgtga caatggggag gacgccgtac gtgcccgcta
     3121 cggtgctgta cttactggcc gaatgcacgg cgcaagaagg cgtcgctgcg gctcagcgtc
     3181 agggttcgcg catcggaagc tgcgacgggc gcctccgccc gggaatcagt tcagcttgat
     3241 ctgaacgtcg acgcccgccg gcagctcgag cttcatgagc gcatccaccg tcttgtcggt
     3301 gggattcatg atgtccatga ggcgcttgtg ggtgcgcagc tcgaactggt cgcgcgcgtc
     3361 cttgtccgcg tgcggggaga tgagcaccgt gaagcgctcc atcttggtcg gcagggggac
     3421 cgggcccttg acggtcgcgc cggtgcgctt ggccgtctcg acgatctcct tggcggagct
     3481 gtcgatcagg cgatggtcaa acgccttcaa acggatacgg atattctggt ttgccatcgc
     3541 ctttgcctct tacttcagga tcttcaccac gacgccggcg ccgacggtct tgccgccctc
     3601 gcggatcgca aagcgcagcc cctgatccat cgtgatcggg ctgatcagct cgatcgtcat
     3661 cttgatgttg tctccgggca tgaccatctc ggcccccgcg ggcagctcga tggccccggt
     3721 gacgtccgtc gtgcggaagt aaaactgcgg ccgatagcct ttgaagaacg gcgtgtgccg
     3781 cccgccctcc tccttcgtca gcacatacac ctcaccctca aagtgcgtgt gcggcgtaat
     3841 gctcccgggc ttcgccagca cctgcccgcg ctcgacttcc tcgcgcttgg tgccgcggag
     3901 aagaacaccg acgttatcgc ccgcctgccc ctcatcgagg agcttgcgga acatctccac
     3961 ccccgtgcac accgtcttgg ccgtcgggcg cagacccacg atctccacct catcgttcac
     4021 ccgcacgatg ccgcgctcga tgcgccccgt caccaccgtc ccgcgacctg agatcgagaa
     4081 cacatcctcc accggcatca gaaacggctt gtcgatctcg cgcttcggga tcggaatgta
     4141 gctgtccatc tcctcgacca gcttcaccac cgagggcacc ccgagctccc ccttgtcccc
     4201 ctccagcgcc ttcaacgcag acccaatcac aatcggggtc gcatccccag gaaacttgta
     4261 cgtcgtcagc agctcccgga cctccatctc caccagctcc aacagc
//
LOCUS       DATLKL010000802         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_133876, whole genome shotgun sequence.
ACCESSION   DATLKL010000802 DATLKL010000000
VERSION     DATLKL010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133876"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VM713_10890"
     CDS             complement(<1..374)
                     /locus_tag="VM713_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84809.1"
                     /translation="MTATLALLTACGGGGGGGGGGSMGPAPPPYSSTTLVQLSKTSTF
                     AAGCDGVAANGTLYTDTAAEPSFVVNPANSMNLVAGWQQNRWSTGGAQGLNHAASFDG
                     GATWTLANAAFSRCTGGGSGNAG"
     gene            complement(418..1722)
                     /locus_tag="VM713_10895"
     CDS             complement(418..1722)
                     /locus_tag="VM713_10895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HVH84810.1"
                     /translation="MRYVFKLPDLGEGTVEAEIVGWRVKPGDTVAEDDVVVEVMTEKA
                     AVEVPAPVSGRVVSTTGEPGDMVPVGAELIVFETEARAVDAPDTAQAAPPGPGSQGSI
                     ATTASDAARGGNGHGAGAALEAHVAAAAARGRVATSPAIRRRAHEAGIDLRQVAGSGP
                     SGRIVSKDLDAFVARGAQGQPVPLRPAPRAVAAAKPAPRAGGGIEEIKVIGLRRLIAQ
                     RMTEAKRNIPHFAYVEELDITELEGLRAHLNSKLERGAPGLTYLPFLALALVRVLKDF
                     PQCNAHYDAERGVIVRHAAVHLSIATQTPDGLKVPVVHDAHKRSLWDLAAEMRRVSEA
                     ARTNKATREELTGSTITITSLGKLGGIASTPIINAPEVAIVGVNRAVERAVVFEGAIA
                     IRRTMNLSSSFDHRFVDGYDAAAMIQALKECLEHPATIFIGA"
     gene            complement(1719..>2149)
                     /locus_tag="VM713_10900"
     CDS             complement(1719..>2149)
                     /locus_tag="VM713_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HVH84811.1"
                     /translation="RGEVPEGHYTVPLGRAEVVRPGKDLSVLTYGTMVHVAEAAVRAT
                     GIDAEIVDVRTMAPLDVETLCTSVCKTGRCVVVHEATRFSGYGAELVATVQEHCFWNL
                     EAPVQRVAGWDTPYPHAFEWDYFPGQARIAAALKQVMEAA"
BASE COUNT          292 a          805 c          735 g          317 t
ORIGIN      
        1 ccggcgttgc cgctgccgcc gccggtgcag cgcgagaagg cggcgttcgc aagcgtccag
       61 gtcgcgccgc cgtcgaatga ggcagcgtgg ttcagaccct gtgcgccgcc ggtcgaccag
      121 cggttctgct gccagcccgc gaccaggttc atggagttgg ccgggttgac gacgaacgac
      181 ggctcggcgg cggtgtcggt gtaaagcgtc ccgttcgccg ccacgccgtc acagcccgcg
      241 gcgaaggtcg aagtcttcga gagctgcacc agcgtcgtcg agctgtaggg cgggggtgcc
      301 gggcccatgc tcccgccgcc gccgccaccc ccgccgcccc cacaggcggt gagcagcgcg
      361 agagtcgcgg tcacagcggg tcgcgccgac atcggtcgct cgcgaaccgg gtgccggtca
      421 ggccccgatg aagatcgtcg ccgggtgctc caggcactct ttcagcgcct ggatcatcgc
      481 cgccgcatcg tagccgtcga cgaagcgatg gtcgaaggaa gaggacaggt tcatcgtgcg
      541 gcggatcgcg atcgctccct cgaacaccac cgcgcgctcg acggcgcgat tcacgccgac
      601 gatcgccacc tccggcgcgt tgatgatcgg cgtgctcgcg atgccgccca gcttgccgag
      661 gctcgtgatg gtaatggtcg agccggtgag ctcctcacgc gtcgccttgt tggtgcgggc
      721 ggcctcgctc acgcggcgca tctcggccgc gaggtcccag agcgagcgct tgtgcgcatc
      781 gtgaaccacc ggcaccttga gcccgtcggg ggtctgcgtg gcgatgctga ggtgcacggc
      841 cgcgtggcgc acgatcacgc cgcgctcggc gtcgtagtgc gcattgcatt gcgggaagtc
      901 cttcagcacc cgcaccagcg cgagcgccag gaacggcagg taggtgaggc ccggcgcgcc
      961 gcgctcgagc ttgctgttga gatgcgccct cagcccctcg agctcggtga tgtcgagctc
     1021 ctcgacgtag gcgaagtggg gaatgttgcg tttcgcctcg gtcatgcgct gggcgatgag
     1081 gcggcgcagg ccgatcacct tgatctcctc gatgccgccg cccgcgcggg gcgcgggctt
     1141 cgccgcagcc accgcccggg gcgcgggccg cagcggcacc ggctgcccct gcgcaccgcg
     1201 cgccacgaag gcgtcgaggt ccttcgagac gatgcggccg ctcggacctg agccggcgac
     1261 ctgccgcagg tcgatgcccg cctcgtgggc gcggcggcgg atcgcgggcg acgtcgccac
     1321 ccgtccgcgc gcggccgccg ccgccacatg cgcttcgagc gcagcgcccg cgccatggcc
     1381 gttgccgccc cgcgccgcgt cagaggcagt cgtcgcgatg ctgccctgcg agcccggccc
     1441 cggcggcgcg gcctgggccg tgtcgggcgc atccaccgcg cgcgcctcgg tctcgaacac
     1501 gatcagctcc gctcccaccg ggaccatgtc gcccggctcg ccggtcgtcg atacgacccg
     1561 cccgctcacc ggcgccggca cctcgaccgc cgccttctcg gtcatcactt ccacgaccac
     1621 atcgtcctcg gccaccgtgt cgccgggctt gacgcgccag ccgacgatct cggcctcgac
     1681 cgtgccctcg ccgagatccg gcagcttgaa cacgtacctc atgcggcctc catcacctgc
     1741 ttcagcgccg cggcgatgcg cgcctgacct gggaaatagt cccattcgaa ggcatgcggg
     1801 tacggcgtat cccagcccgc gacccgctgc accggggcct cgagattcca gaagcagtgc
     1861 tcctgaacgg tcgcaaccag ctccgcgccg tagccgctga aacgcgtcgc ctcgtgcacc
     1921 accacgcagc gcccggtctt gcagaccgag gtgcacagcg tctcgacatc gagcggcgcc
     1981 atggtgcgca cgtcgacgat ctcagcatcg atgccggtgg cgcgcacggc ggcctcggcg
     2041 acgtgcacca tggtgccgta ggtgagtacg ctcagatcct tccccggacg caccacctcg
     2101 gcgcgtccca gcggcacggt gtagtgaccc tccggcacct cgccgcgcg
//
LOCUS       DATLKL010000803         1864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_178893, whole genome shotgun sequence.
ACCESSION   DATLKL010000803 DATLKL010000000
VERSION     DATLKL010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178893"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1732
                     /gene="parC"
                     /locus_tag="VM713_10905"
     CDS             <1..1732
                     /gene="parC"
                     /locus_tag="VM713_10905"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HVH84812.1"
                     /translation="AVGMATDIPPHNLREVAAAAIHLLDEPDATTAALMKHVKGPDLP
                     TGAEIISPRADLKEFYDKGVGSFKARATWEIEEGNAVITAFPYQVSPTRVMEQIAEQM
                     RAKKLPMIDDLRDESDHDNPTRLVLVPRSNRVDLTEVMNHLFATTDLERNYRVNLNII
                     ALDGRPRVMGLKEILGEWLEFRTRTVTRRLEHRLEKVGKRLHILDGLLIAFLNLDEVI
                     RIIRREDEPKPVLMKRFKLTDEQTEEILNTRLRHLAKLEEMKIREEQKTLAAERAELE
                     GLLKSKAKLRKLIAAEIRADAEKYGDERRTKIIEREAAQAIDEASLIPNEPVTVVLSK
                     GGWVRSAKGHEVDVATLSYKGGDAFQALARGRSLQSAVFIDSTGRTYTLPAHSLPSAR
                     GYGEPLSGRLDPPDGAKFAGVMIGEPDDLWLLASDAGYGFAARLKDLVTDRRAGKTVL
                     SVPENAQVLPPAPVPSADSLVAVVSSEGKLLVFPVAEVPEMPRGKGLKLYDIPGKKAR
                     TRTELMTALAVVPPEASLALWSGETSKLIEWRDLKDYRGQRAQRGAVLKRGWPRKIER
                     MEAQLPAPKT"
BASE COUNT          339 a          652 c          621 g          252 t
ORIGIN      
        1 cgccgtcggc atggcgaccg atatcccgcc gcacaacctg cgcgaggttg cggccgccgc
       61 catccacctg ctggatgagc ccgacgccac gaccgccgcg ctcatgaagc acgtcaaggg
      121 cccggacctg ccgaccggag ccgagatcat ctcgccgcgc gccgatctca aggagttcta
      181 cgacaagggc gtcggcagct tcaaggcgcg cgccacctgg gagatcgagg agggcaacgc
      241 ggtcatcacc gcctttccct accaggtgtc gccgacgcgt gtgatggagc agatcgccga
      301 gcagatgcgc gccaagaagc tgccgatgat cgacgatctg cgcgacgaat ccgaccacga
      361 caacccgacc cggctcgtgc tcgtgccgcg ctcgaaccgc gtcgacctca ccgaggtcat
      421 gaatcacctg tttgcgacca ccgacctcga gcgcaactac cgcgtcaatc tcaacatcat
      481 cgcgctcgac ggccggcccc gggtcatggg tctgaaggaa atcctgggcg aatggctcga
      541 gttccgcacc aggaccgtca cgcggcgcct cgagcaccgg ctcgagaagg tcggcaagcg
      601 gctgcacatt ctcgacgggc tcctcatcgc cttcctcaat ctcgatgagg tgatccgcat
      661 catccgccgc gaggatgagc cgaagccggt gctcatgaag cgcttcaagc tcaccgacga
      721 gcagaccgag gagatcctca acacgcgcct gcggcacctc gccaagctcg aggagatgaa
      781 gatccgcgag gagcagaaga cgctcgccgc cgagcgcgca gagctcgagg ggctgctgaa
      841 gagcaaagcg aagctgagga agctcatcgc cgccgagatc cgcgccgatg ccgagaaata
      901 cggcgacgag cggcgcacca agatcatcga gcgcgaggcc gcccaggcga tcgatgaggc
      961 gagcctcatc cccaacgagc cggtgacggt ggtgctctcg aagggcggct gggtgcgctc
     1021 ggccaagggg cacgaggtcg acgtggcgac gctctcctac aagggcggcg atgccttcca
     1081 ggcgctcgcg cgcggccgga gcctgcagag cgcggtgttc atcgacagca ccggacgcac
     1141 ctacacgctg cccgcgcact cgctcccttc ggcgcgcggc tacggcgaac cgctctcggg
     1201 gcgcctcgat ccgcccgacg gcgccaagtt cgcgggcgtc atgatcggcg agcccgacga
     1261 cctgtggctg ctcgcgagcg atgccggcta cggcttcgcg gcgcgcctca aggacctcgt
     1321 gaccgaccgg cgcgccggca agacggtact gagtgtcccg gagaacgcgc aggtactccc
     1381 gccggcgccg gtgccctcgg ccgactcgct cgtcgccgtc gtgagcagcg agggcaagct
     1441 cctcgtattc ccggtggcgg aggtgcccga gatgccgcgc ggcaagggcc tgaagctcta
     1501 cgacatcccc ggcaagaagg cacgcacgcg caccgagctc atgacggcgc tcgcggtcgt
     1561 gccgcccgag gcgagcctcg ctctctggtc gggagagacc agtaagctca tcgagtggcg
     1621 cgatctcaag gactatcgcg ggcagcgtgc ccagcgcggc gcggtgttga agcgcggctg
     1681 gccgcgcaag atcgagcgca tggaggcgca gctgccggcg ccgaagacct gaagcacgtg
     1741 cgcgccggga gtccgcggcg ctcggtcagc gcccggcgct cagcaccact tcctcgggct
     1801 cgttgacgaa atagccctgg atgtactgca cgccgagctg ccacagcacc gccatggtgt
     1861 tggc
//
LOCUS       DATLKL010000804         2468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_145160, whole genome shotgun sequence.
ACCESSION   DATLKL010000804 DATLKL010000000
VERSION     DATLKL010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145160"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VM713_10910"
     CDS             <1..501
                     /locus_tag="VM713_10910"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVH84813.1"
                     /translation="MGFGLPAAIGAQIACPKRLVIDIDGDSSIRMNIGELETATTYNL
                     PVKVVVLNNFGDGMVKQWQKLFFKGRLSASDRSLHKKDFVKAAQADGFRYAARLEHKA
                     DVPRVIAEFVGFKGPAFLEVMIDPDAGVYPMVGPGQTYEDMITGEHIPSRTKVDIKTP
                     GPSEMF"
     gene            501..>2468
                     /locus_tag="VM713_10915"
     CDS             501..>2468
                     /locus_tag="VM713_10915"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVH84814.1"
                     /translation="MRPLSARAAPLAACLLAAAAAAPAATPAAGGAREDEILMLGNRA
                     GAQSVRTGSDGVTHAEYSFNDRGRGDHIVATWKVDAAGVLTEYEGRGNDYMKAPVTES
                     FQLAAGKATWSNRSEHGSTAVTGEAFYVPVNGPPEIVGVLARALLKAPGHRLALLPAG
                     EAALEKVELAAEDAARSTGPHGALALYAVSGLDFSPTPIWLEADGTTAASIVFSGWFS
                     VLPAALHEQQPQLLAAQEHAAAAWAARLAREATHVPAGDVVIRNARLFDPRDLTVTPG
                     TSVLVRGAHIVRVAADADVTADAKAEAIDAHGRFLMPGLWDNHQHFGDHDGALDLANG
                     VTSARDMANDTDRFLERVARFDAGTELGPRVWKSGIIDGAGPYTGPTKMRIETAAQAV
                     ADVDWYADHGYGQIKIYSSVPPPIVPVIAAEAHARGLRVSGHVPAFMSAEQFVAAGAD
                     EIQHLNFIVLDFLYPAVQETRNMNRFTAVAAHARDFPPSAPQVRDFIAYLARHHTVLD
                     PTVNIFESEFCGDPAALTPGLEAVAPRMPPQVRRGLLSSALVVPKGEEAAYREALPAM
                     LSLLKALHDAGVTIIPGTDSLPGYELHHELSLYVRAGIPPPEVLRMATLTSATVIGAN
                     GERGVIAPGKLADLILVDGDPSVRIGDIDRID"
BASE COUNT          384 a          895 c          833 g          356 t
ORIGIN      
        1 atgggcttcg gtctgccggc ggcgatcggt gcgcagatcg cgtgcccgaa gcgcctggtg
       61 atcgacatcg atggcgactc cagcatccgc atgaacatcg gtgagctgga gaccgcgacg
      121 acctacaacc tgccggtgaa ggtcgtggtg ctcaacaatt tcggcgacgg catggtgaag
      181 cagtggcaga agctgttctt caagggacgg ctgtccgcga gcgaccgctc gctgcacaag
      241 aaggatttcg tcaaggcggc gcaggcggat ggcttccgct acgcggcgcg cctcgagcac
      301 aaggcggacg tgccgcgcgt catcgccgag ttcgtcggct ttaaggggcc agcctttctc
      361 gaggtgatga tcgaccccga tgccggcgtc tatccgatgg tcggccccgg gcagacctac
      421 gaggacatga tcaccggcga gcacattcca tcccgcacca aggtcgacat caagactccc
      481 gggccctcgg agatgttctg atgagacccc tgtcagcgcg cgccgcacct ctcgccgcct
      541 gcctgctcgc cgcggccgcc gcggcgcccg ccgccacgcc cgcggccgga ggcgcacgag
      601 aggacgagat cctgatgctc ggcaatcgcg cgggcgcgca gagcgtccgc accggcagcg
      661 acggcgtcac gcacgcggag tactccttca acgaccgcgg ccgcggcgac catatcgtcg
      721 cgacctggaa ggtcgacgcc gctggcgtcc tcaccgaata cgagggtcgc ggcaacgact
      781 atatgaaggc gccggtgacc gagagcttcc agctcgccgc cggcaaggcc acctggagca
      841 accgctcaga gcacggcagc acggcggtca cgggcgaggc gttctacgtg cccgtgaatg
      901 gaccgcccga gatcgtcggc gtgctcgcgc gggcgctcct caaagccccg gggcatcgcc
      961 tcgcgctcct gcccgccggc gaggcggctc tcgagaaggt cgagctggcc gcggaggacg
     1021 ccgcccgctc gaccggccct cacggcgcac tggccctcta cgccgtgagc gggctcgact
     1081 tctccccgac gccgatctgg ctcgaggccg atgggacgac ggcagcctcg atcgtcttct
     1141 ccggctggtt ctcggtgctg ccagcggcgc tgcacgagca gcagccgcag ctcctcgcgg
     1201 cgcaggagca cgccgctgcc gcctgggcgg cacgcctggc gcgcgaggcg acgcacgtgc
     1261 cggccggcga cgtcgtcatc cgcaacgcgc ggctcttcga tccgcgcgat ctcacggtca
     1321 cgccgggaac gagtgtgctg gtgcgcgggg cgcatatcgt gcgggtcgcc gcggacgccg
     1381 acgtgactgc cgacgccaag gccgaggcga tcgacgcaca cggccgcttc ctcatgccgg
     1441 ggctctggga caaccaccag catttcggcg accacgacgg cgcgctcgat ctcgccaacg
     1501 gcgtcacgag cgcccgcgac atggcgaacg acaccgaccg gttcctcgag cgcgtggcgc
     1561 gtttcgacgc cggcaccgag ctcggcccgc gggtgtggaa gtccggcatc atcgacggcg
     1621 cgggcccgta caccgggccg accaagatgc gtatcgagac cgccgcgcag gccgtcgcgg
     1681 acgtcgactg gtacgccgac cacggctacg ggcagatcaa gatctactcc tcggtcccgc
     1741 cgcccatcgt gccggtaatc gccgccgagg cgcatgcgcg cgggctgcgc gtcagcgggc
     1801 atgtgccggc gttcatgagc gccgagcagt tcgtggcggc cggtgccgac gagatccagc
     1861 acctcaactt catcgtgctc gacttcctct atccggcggt gcaggagacg cgcaacatga
     1921 accgcttcac cgcggtcgct gcgcacgccc gcgacttccc gcccagcgcg ccgcaggtgc
     1981 gggacttcat cgcctacctc gcgcgtcacc acacggtgct cgatcccacc gtgaacatct
     2041 tcgagtccga gttctgcggc gacccggcgg cgttaacccc gggtctcgag gcggtcgcgc
     2101 cgcgtatgcc gccgcaggtg cggcgcggac tcctctcgag cgcgctcgtg gtgccgaagg
     2161 gcgaggaagc cgcctatcgc gaagcgctgc cggcgatgct tagcctcctc aaggcgctgc
     2221 acgatgccgg ggtgacgatc attcccggta ccgactcgct gcccggctac gagctgcacc
     2281 acgagctctc gctctacgtg cgggccggca ttccgccgcc cgaggtgctg cgcatggcga
     2341 ccctcacctc ggcgacggtc atcggagcga acggcgagcg cggcgtgatc gcccctggca
     2401 agctcgccga tctcatcctc gtcgacggcg acccgagcgt gcgcatcggc gacatcgacc
     2461 gcatcgac
//
LOCUS       DATLKL010000805         3506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_190367, whole genome shotgun sequence.
ACCESSION   DATLKL010000805 DATLKL010000000
VERSION     DATLKL010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190367"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1656
                     /locus_tag="VM713_10920"
     CDS             70..1656
                     /locus_tag="VM713_10920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH84815.1"
                     /translation="MDGIVFEQRRNTLIFWCGCALVTAGVLAHLPMFLMGRATGFVLA
                     GMPMGFGMLLGMAAIVLGVGAAAYGLLPAAPRATSVALDVTAPLESARLGPAHWRVAG
                     LLALALVIDIMKPASLGFVTPGMKSEYHVGAQTVAWLPFAALAGTVVGSVVWGVLADV
                     YGRRASIVLSSVMFVGTSICGAMPDFWWNVGMCFMMGAAAGGMLPVAYALLAEVMPTR
                     HRGWSLVLVGGVGALGGFFAASFLSALLQPTFGWRIMWLLNLPTGLLLVVLSPLLPES
                     ARFLQETGRSELARVTLARFGVVLAPAATRAATVPHESHAPVPPLAHGFLGITAALTL
                     AALAWGMVNFGLLLWLPSALVAQGRSVAASSALIAKSTLLAAPTIVVCAWLFSAWSTK
                     WSLIAVMGVMVLGLAGLTLPEVGSGGPSGPVVPLALVILGSCGVIAMLLPYAAENYPL
                     LIRGRATGWVAACSKFGGLAAQTLSVLGGVPSLTVVILFIAAPAVVASLLIALFGHET
                     RGRGLGELDLAHRIGSRDST"
     gene            complement(1707..2096)
                     /locus_tag="VM713_10925"
     CDS             complement(1707..2096)
                     /locus_tag="VM713_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVH84816.1"
                     /translation="MAYVTVINEHQHLQQVVSGHHHLTADEAVAAGGSDAGFGPRELL
                     LASLGTCTAVALRKHAAQNDWTLGKITVGVRWSRDAEGKEHIERRLSFAKPLSDAQKE
                     RLLEVAEETPVTGLLRAAIRIQSLVVD"
     gene            complement(2071..3105)
                     /locus_tag="VM713_10930"
     CDS             complement(2071..3105)
                     /locus_tag="VM713_10930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVH84817.1"
                     /translation="MRSCEVIIVGGGPAGSSAAWRLRRAGADVLILDRERFPRLKLCA
                     GWITPEVVRELEMDLDEYPHRLLTFPRLRVHYGRLNRPVPCVQHSIRRFEWDAWLLER
                     AGAPVEQHNVREVVADGDGYVIDGAWRCRYLVGAGGTRCPVYRNLFKELNPRAHELQI
                     VTLEHEIEYDWQDGDCHLWFCEEGLPGYSWYVPKESGWLNVGVGGFAERIKTAGQDIK
                     THWAHFAEKLGGGLTRGAHYDPVGYSYFLRGQVEVVRRDNAFITGDAAGLASRDLGEG
                     IGPAIRSGLRAAEAILHGAPYRLDDVTGLSVASIARSMLSRAVRPLGPHGRRRASNGG
                     TGHGLRHGNQ"
     gene            complement(3113..3265)
                     /locus_tag="VM713_10935"
     CDS             complement(3113..3265)
                     /locus_tag="VM713_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84818.1"
                     /translation="MFDEKFVRMWRLYLAGSVAGFSTGTLQLFQVVFATADNNDVPWT
                     RAHLYT"
     gene            3306..>3506
                     /locus_tag="VM713_10940"
     CDS             3306..>3506
                     /locus_tag="VM713_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84819.1"
                     /translation="MTQRLRVVQAQVLDVQHRQVPGLEDLRHLAQGRRVGAGEDALLD
                     PWLQPRRVVAADRMNEAAPVAPE"
BASE COUNT          476 a         1255 c         1172 g          603 t
ORIGIN      
        1 gtcactccgc cgtcgactcg cgatcataac cgggcccttc cgcccatata tatagaataa
       61 gatcgtcgca tggacggcat cgttttcgag cagcggcgca acacgctcat cttctggtgc
      121 ggctgcgcgc tggtcacagc gggcgtgctg gcgcatctgc cgatgtttct catggggcgg
      181 gctaccggct tcgtgctcgc cggcatgccc atgggtttcg gcatgctgct cggcatggcg
      241 gcgatcgttc tcggggtcgg cgccgccgct tacggcctgc tgcccgccgc gccgcgcgcc
      301 acctccgtgg cgcttgatgt gaccgcgccg ctcgagagcg cgcgcctcgg cccggcccac
      361 tggcgggtcg cggggctgct cgcgctcgcg ctggtcatcg acatcatgaa gccggccagc
      421 ctcggcttcg tcacccccgg catgaagtcc gagtaccacg tcggcgcgca gacggtcgcc
      481 tggctgccgt tcgccgcgct cgcgggcacg gtggtgggct cggtggtgtg gggcgtgctg
      541 gccgatgtct acgggcggcg cgcctcgatc gtcctgtcct cggtgatgtt cgtcggcacc
      601 tcgatctgcg gcgcgatgcc tgatttctgg tggaacgtcg gcatgtgctt catgatgggc
      661 gcggccgccg gcggcatgct gccggtcgcc tacgccctgc tcgccgaggt catgccgacc
      721 cgccaccgcg gctggagcct ggtgctggtg ggcggtgttg gtgcgctcgg cggcttcttc
      781 gccgcgagct tcctctcggc gctcctgcag ccgacctttg gctggcgcat catgtggttg
      841 ctcaacctgc cgaccgggct cctgctggtg gtgttaagcc cgctgctgcc ggagtccgcg
      901 cgattcctgc aggagaccgg gcgcagcgag cttgcgcgcg tgacgctcgc gcgcttcggc
      961 gtagtgctcg cgcccgccgc gactcgcgcc gccaccgtgc cgcatgagag ccacgcgccg
     1021 gtgccgcccc tcgcgcacgg attcctcggc atcaccgccg ccctcacgct cgccgcgctc
     1081 gcctggggaa tggtgaactt cggtctcctg ctgtggctcc cgagcgcact cgtcgcgcag
     1141 gggcgcagcg tcgccgcatc gagcgcgctg atcgcaaagt ccacgctgct cgccgcgccg
     1201 accatcgtcg tctgcgcgtg gctgttcagc gcctggagca ccaagtggtc actcatcgcg
     1261 gtgatgggcg tcatggtgct cgggctcgcc ggcctgacat tgcccgaggt cggatccggc
     1321 ggaccctccg gcccggtggt gccgctggcg ctcgtcatcc tcggctcctg cggcgtcatc
     1381 gccatgctgc tgccctacgc cgccgaaaac tacccgctcc tcatccgcgg ccgcgccacc
     1441 ggctgggtgg ccgcgtgcag caagttcggc ggccttgccg cgcagacgct cagcgtcctc
     1501 ggcggagtgc cgtcgctcac cgtggtcatt ctgtttatcg ctgcccccgc ggtcgtcgcc
     1561 tctctcctca tcgcgctctt cggccacgag acgcgcggtc gcggcctcgg cgagctcgat
     1621 ctcgcgcacc gcatcggcag ccgagactca acttaaggcg ctataccgac agcattaccg
     1681 tcagcgtgaa ctactgacgc agcgcgtcag tccacgacca gagactggat tctgattgcg
     1741 gcgcgcagca ggccggtcac gggcgtctcc tcggcgacct caaggagccg ctccttctgg
     1801 gcgtcgctga gcggcttggc gaaggaaagg cgccgctcga tgtgctcctt gccctcggca
     1861 tcgcgcgacc agcgcacgcc gaccgtgatc ttgccgagcg tccagtcatt ctgtgcagcg
     1921 tgcttgcgca gggccaccgc ggtgcaggta ccgaggctcg cgagcagcag ctcgcgcggc
     1981 ccgaagcccg catcgctccc tccggcagcg accgcctcgt cggccgtcag atggtgatga
     2041 ccggagacga cctgctgtaa gtgctggtgc tcattgatta ccgtgacgta ggccatgccc
     2101 cgttcctccg ttgctcgcgc gccgccgccc gtgcggaccg agcggccgca ccgcgcggct
     2161 cagcatactc ctcgcgatgc tcgcgacact caggccggtc acgtcgtcga gtcggtaggg
     2221 tgcgccgtgg aggatggcct cagcggcccg cagcccgctg cggatcgccg gcccgatgcc
     2281 ctcgcccagg tcacggctgg cgagtccggc cgcatcgccg gtgatgaagg cattgtcgcg
     2341 ccgcacgacc tcgacctgcc cgcgcaggaa atagctgtag ccaaccgggt cgtagtgcgc
     2401 accgcgcgtc agtccgccgc cgagcttctc ggcgaagtgc gcccagtgtg tcttgatgtc
     2461 ctggcctgcg gtcttgatgc gctcggcaaa cccgccgacg ccgacgttga gccagccgct
     2521 ctccttcggc acgtaccagg agtacccggg cagtccctcc tcgcagaacc acaggtggca
     2581 gtcgccgtcc tgccagtcgt actcgatctc gtgctcgagc gtcacgatct gcagctcgtg
     2641 tgcgcgcgga ttgagctcct tgaagaggtt gcggtacacc gggcagcgcg tgccgccggc
     2701 gccgacgaga tagcggcagc gccaggcgcc gtcgatcacg taaccatcgc cgtcggccac
     2761 gacctcgcgc acgttgtgct gctcgaccgg cgcgccggcg cgctcgagca gccacgcgtc
     2821 ccattcgaag cgccgaatcg agtgctgcac acacggcacc ggccggttta gccgcccgta
     2881 gtgcacccgc agtcgcggaa aagtgagcag gcgatgcgga tactcgtcga gatccatctc
     2941 gagctcgcgc accacttcgg gagtgatcca gcccgcacag agcttcagcc gcgggaagcg
     3001 ctcgcggtcg agaatgagca cgtcggcacc ggcgcgtctg agacgccacg cggcgctcga
     3061 gcccgcgggc ccgccgccga cgatgatgac ctcgcacgag cgcatgggac ggtcaggtgt
     3121 agaggtgcgc gcgagtccag ggaacgtcgt tgttgtcggc agtcgcaaac accacctgga
     3181 agagctgcag ggtgccggtg gagaaccccg cgaccgagcc cgccagatac agccgccaca
     3241 tacgcacgaa tttctcgtcg aacatcgccc gcacccgctc ggccgctgac tcgtagggcc
     3301 agccagtgac gcagcgtctg cgcgtagtgc aggcgcaggt tctcgacgtc cagcaccgac
     3361 aggtcccagg gctcgaagat ctgcgacatc tcgctcaagg ccggcgggtg ggcgccgggg
     3421 aagatgcgct tctcgatcca tggctgcagc cgcgccgggt ggttgcggcc gatcgaatga
     3481 atgaggccgc gcccgttgca cccgag
//
LOCUS       DATLKL010000806         1735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_178952, whole genome shotgun sequence.
ACCESSION   DATLKL010000806 DATLKL010000000
VERSION     DATLKL010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178952"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VM713_10945"
     CDS             complement(<1..522)
                     /locus_tag="VM713_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84820.1"
                     /translation="MDRPLLALVRLLAPLAWLALAATARADGWELDLDGRLVSSNADP
                     SYTQGGISAVRFDQEQSGLRLGRARFALTESLGEFWSAHLDASAWGDHDRIPAGLTEA
                     YLQFRPYPFAGYRLRIKAGAFYPPVSLENRASGWESPYTLSSSALDSWLAIEVRTVGV
                     EAQLDWLGTRLGHD"
     gene            complement(524..1150)
                     /locus_tag="VM713_10950"
     CDS             complement(524..1150)
                     /locus_tag="VM713_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HVH84821.1"
                     /translation="MAALLCALSAGGPARAATLVITVLTADGRPLAGAVVTTHPLDAP
                     SHKPQPIKAVMDQVNRAFAPDLLVIPVGSTVTFPNSDSVSHQIYSFSPAKRFQLPLYR
                     GTPYPPVHFEQAGVVTLGCNIHDEMVGYLVVTDAAYYGRTDTKGTWSDEVARGRYRIT
                     IWHPRMREQAADLERELTIGEADHAELTLRLTKSLTPAPLAPHSSWDY"
     gene            1278..>1735
                     /locus_tag="VM713_10955"
     CDS             1278..>1735
                     /locus_tag="VM713_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84822.1"
                     /translation="MGSFRKRLLVLIIGLVIVTQTVTLAAVLVSTRGTVEQRAKEQLG
                     SGTSLALQLMRFRAGELANGVSVTAADFGFREAVASGEPLTILSAARNNARRIGADMV
                     MVLDTRGTVVAATVPVDADAALSIEQLLRDPGARNQPAFIILGPSAYELVL"
BASE COUNT          272 a          558 c          637 g          268 t
ORIGIN      
        1 gtcgtgcccc agtcgcgtgc cgagccagtc gagctgtgcc tcgacaccca cggtgcgcac
       61 ttcgatggcg agccagctgt cgagcgccga agacgagagc gtgtaggggg actcccagcc
      121 cgaggcgcgg ttctcgagcg acaccggcgg atagaaggcg ccggccttga tgcgcagccg
      181 gtaaccggcg aacgggtagg ggcggaactg caagtacgct tcggtgagcc cggcggggat
      241 gcggtcgtga tcgccccatg cggaggcatc gaggtgcgcg ctccagaact cgccgagcga
      301 ctcggtgagc gcgaagcgcg cgcggccgag cctgaggccc gactgctcct gatcgaagcg
      361 cacggcgctg atgccgccct gagtgtaaga ggggtcggcg ttcgagctca ccaggcgccc
      421 gtcgagatcg agctcccagc cgtcggcgcg ggccgtggcg gcgagggcga gccaggcgag
      481 cggtgcgagc aacctcacga gagccaacag cgggcgatcc atctcaatag tcccaggagg
      541 aatgcggcgc gagcggcgcg ggtgtgagcg acttcgtcag acgcagggtg agctcggcat
      601 ggtcggcttc gccgatcgtg agctcgcgct cgaggtccgc cgcctgctcg cgcatgcgcg
      661 ggtgccagat cgtgatgcgg tagcggccgc gcgccacctc gtcggaccag gtccccttgg
      721 tgtcggtgcg cccgtagtag gcggcgtcgg tgaccacgag gtaaccgacc atctcgtcgt
      781 ggatgttgca gccgagggtg accacacccg cctgctcgaa gtgcacgggc ggatagggcg
      841 taccgcggta gagcggcagc tgaaagcgct tcgctggaga gaacgagtag atctgatggc
      901 tcaccgagtc gctgttcggg aacgtcacgg tggagccgac cggaatcacc agcaggtccg
      961 gcgcaaaggc gcggtttacc tggtccatga cggccttgat gggttgcggc ttatgcgacg
     1021 gggcatcgag cgggtgcgtc gtcaccaccg cgcctgccag cggccggccg tcggcagtga
     1081 ggacggtgat gaccagggtt gccgcacggg cgggaccccc ggcggagagc gcgcacagga
     1141 gggccgccac gccagccagg gcgcggacgg cggttctgtg aatgcgcgca cgtgctcgag
     1201 gtctcattcc cggactcatg tcctcgcacc ctccatggcg ggacacctag gatgtggcgg
     1261 gcattcgagc gtttttgatg ggcagtttcc gcaaacggct tctcgtgctg atcatcgggc
     1321 tggtcatcgt cacccaaact gtgacgctgg ccgcagtgct ggtcagcacc cggggaacgg
     1381 tggagcagcg ggccaaggag cagctcgggt ccggcacctc gctcgcgctg cagctcatgc
     1441 gcttccgcgc cggggagctc gccaacggcg tgtccgtgac ggcggcggat ttcggcttcc
     1501 gcgaagcggt cgccagtggt gagccgctca ccatcctgtc ggcggcccgt aataatgccc
     1561 ggcgcatcgg cgccgacatg gtcatggtgc tcgatacccg cggcacggtg gtcgcggcga
     1621 ccgtcccggt cgacgccgac gcggctctct ccatcgagca gctgctgcgc gaccccggtg
     1681 cgcgtaacca gccggccttc atcattctcg gcccgagcgc ctacgagctg gtgct
//
LOCUS       DATLKL010000807         1981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_178957, whole genome shotgun sequence.
ACCESSION   DATLKL010000807 DATLKL010000000
VERSION     DATLKL010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178957"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..543)
                     /locus_tag="VM713_10960"
     CDS             complement(55..543)
                     /locus_tag="VM713_10960"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HVH84823.1"
                     /translation="MKKAVDPRAYSRIVVDGVTQAPARTMLRAVGFRDEDFAKPQIGV
                     ASTWANLTPCNMHIGALADAAAAGVDGAGGKSVLFNTITVSDGISMGTPGMRYSLVSR
                     EIIADSIEAVVAAEGFDGFVALGGCDKNMPGCAMAMARLNRYTKCTWVPGGACRRKVI
                     GG"
     gene            917..1912
                     /gene="ilvC"
                     /locus_tag="VM713_10965"
     CDS             917..1912
                     /gene="ilvC"
                     /locus_tag="VM713_10965"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010158896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HVH84824.1"
                     /translation="MRLYSQKDVDRKALKGARIAILGYGSQGRAHALNLKDSGFDVVV
                     GVRKGGASWKKARRDGLEVAEPAAAAASADLVAMLVPDLAQKALYQEVKKGLKKGATL
                     LFAHGFNIHFRQVKPRPDLDVVLIAPKGPGDLVRRQYQQGRGVPCLLAVAQDATGKAH
                     ARALAYAHGIGGTRGGVLATTFAEETETDLFGEQAVLCGGTTELVVKGFETLVEAGYQ
                     PEVAYYECLHELKLIVDLLHEGGIAKMHQFISETAKYGDLTRGPRIVGERVKKQMRKV
                     LKEVQSGKFARQWIKENQTGKANYQKLLAADIDRQIEKVGAGLRARMPWLNERRA"
BASE COUNT          368 a          650 c          666 g          297 t
ORIGIN      
        1 tgaaccctgc atcgtgaacg taatcggcga gcagcggcgc tatttcccgg tacgttagcc
       61 accgattacc ttcctccggc acgcgcctcc aggaacccag gtgcacttcg tatatcggtt
      121 gagcctcgcc atggccatgg cacagccggg catgttcttg tcgcagccgc cgagcgcgac
      181 gaagccgtcg aagccctccg ccgcaacgac cgcctcgatc gagtccgcga tgatctcccg
      241 ggacacgagc gagtaacgca tgcccggggt gcccatcgag atcccgtcgg acacggtgat
      301 cgtattgaaa agaacgctct tgccgccggc accgtcaacg cctgccgcag cggcgtcggc
      361 caaagccccg atgtgcatgt tgcagggcgt caggttggcc caggtggagg ccaccccgat
      421 ctggggcttg gcgaaatcct cgtcgcggaa gcccacggca cgcagcatgg tgcgggccgg
      481 cgcctgcgtg accccgtcga ccacgatgcg cgagtacgcc cgcgggtcca ctgctttttt
      541 catggcgctg agattagcac cgagcgggaa gcgcgctcgc gcccgctgcg gctcgagctc
      601 gagaggtcgc gctcaacgcg ttgacaagct cgcggttcct gtgctcccat cgggccaaca
      661 ggcttggttc ccgtgacacc cgacgcaaca gcgcctaaca cccccgccgc ggcactcgcc
      721 gaacggctgg cgctgcttat cggactgccc gcactgctgc tccttaccgg ggcaaagcgg
      781 tgtggggcac gcgcgaacgc ctgatgtaag caggcacgag cgagagagcg aagagaactc
      841 aagaccccgc accggccgaa aagccgctgc ggggtttttt acgtttgcag cggccgtgcg
      901 ccgcaggagt gacgcgatga ggctctattc acagaaggac gtcgaccgca aggcgctgaa
      961 gggcgcgcgc atcgccatcc tcggttacgg cagccagggc cgcgcgcacg cgctgaatct
     1021 caaagactcg ggcttcgacg tcgtggtcgg agtgagaaaa ggtggcgcga gctggaagaa
     1081 ggcgcgccgc gacggcctcg aggtcgcaga gcccgccgcg gccgccgcga gcgccgacct
     1141 ggtcgcgatg ctggtgccgg atctggcgca gaaggcgctc taccaggagg tgaagaaggg
     1201 cctgaagaag ggcgcgacgc tgctgttcgc ccatggattc aacattcact tccgccaggt
     1261 caagccgcgg cccgacctcg acgtggtgtt gatcgcgccc aaggggccgg gagacctggt
     1321 gcgccgccag taccagcagg ggcgcggcgt gccgtgcctg ctcgccgtcg cgcaggatgc
     1381 caccggcaag gcgcacgccc gcgccctcgc ctacgcgcac ggcatcggcg gcacgcgcgg
     1441 cggagtgctc gcgaccacct tcgccgagga gaccgagacc gatctcttcg gcgagcaggc
     1501 ggtgctatgc ggcggaacta ccgagctcgt cgtgaagggc ttcgagacgc tggtcgaagc
     1561 gggctatcag cccgaggtcg cttactacga gtgcctgcac gagctgaagc tcatcgtcga
     1621 ccttctgcac gagggcggga tagccaagat gcatcagttc atctccgaga ccgccaagta
     1681 cggtgatctc acccgcggcc cgcgcatcgt gggcgagcgt gtcaagaagc agatgcgcaa
     1741 ggtgctcaag gaggtgcaga gcggcaagtt tgcccggcag tggatcaagg agaaccagac
     1801 cggcaaggcg aactaccaga agctgctggc cgccgacatc gaccggcaga tcgagaaagt
     1861 cggcgcgggc ttacgcgcca ggatgccgtg gctgaacgag cggcgcgcgt gaacgagcgc
     1921 gaccctcgca cgcccttttc gaggtgatgc acatgagcag taatcccgac cgggtcctga
     1981 t
//
LOCUS       DATLKL010000808         4610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_20906, whole genome shotgun sequence.
ACCESSION   DATLKL010000808 DATLKL010000000
VERSION     DATLKL010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20906"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1787
                     /locus_tag="VM713_10970"
     CDS             <1..1787
                     /locus_tag="VM713_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVH84825.1"
                     /translation="YATAVVVAVFLPELFTSSVEGRFVGPLALAFIFVVLASLLVAMT
                     STPALCALLLRRHDERPESHWLQILKRWQRLSVAAVGRHLRIATLVLAALVVIALGAL
                     PFLGGTFMPDFREGHFVMQVSSSITGTSLEQMAELGKRVSAEVLAAPYVQTIEQQIGR
                     AELSEDTWGPHRSEFHVELKPDAPVDQQAAQEELRGILARYPGIQSEVVTFLGDRISE
                     SLSGETAQVAIKVCGDDLDVLDATGAKIAAALGKVPGIVDLQFKRQSGTPTIAIQLVP
                     QALAAAGLKAQDVLDTIETAYAGAQVGQTFHGTRTVDVDVLLPESWRRQPALVGRLMI
                     ASPLGPVPLAQVARIDVTQDRYSIQHEGGQRLVTVTFNVRGASLQSVVAEAQRTVASS
                     VALLPGVYLEYAGAAAAERQTRNELLLYSAIAMALILMILFTSFHWRVNSWLVLANLP
                     FSLIGSIMAIAVTGVGISLGTVVGLVTVFGVSARNAILQLAHYEHLVEVEGVSWGPAL
                     VLRGANERLVPILMTAAVTALGLAPLAFGLHRPGQEIEGPMAVTVLGGLVSSTLLNLV
                     VLPALAERYIQGHPRTDRELTAPSEGAA"
     gene            1859..3118
                     /locus_tag="VM713_10975"
                     /pseudo
     CDS             1859..3118
                     /locus_tag="VM713_10975"
                     /inference="COORDINATES: protein motif:HMM:NF015355.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
     gene            complement(3081..4493)
                     /locus_tag="VM713_10980"
     CDS             complement(3081..4493)
                     /locus_tag="VM713_10980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH84826.1"
                     /translation="MKSIGARLALWYAAASTVTLACLFVVGYYLLRGYLIHGLDLLNQ
                     SEYQELKARLGPDVSVLSSAQIEQRIRDTTEAAATLFYIDIHRHGTGTIFYSSNLNGR
                     ALPDVKGAHRYDVSWERGPLRVAEFILPPYDVTIATPATHVGEVMEGYAEVGAALLAL
                     MLAVSIAIGLALSRLALRPVRLIRATASRIGSENLSERIAVPEVKDEIADLASLLNAM
                     FDRLEASFKNVRRFAADASHELKTPLSLVRLQAEKLLASGTLGHEGEDGVQTLLEELL
                     RLNGTIDDLLFLSRAESRSIVLQLAPHDAAALLANFSVDAQVLAEHHGVHFSYSHEGD
                     GLPVCEPRRIRRVLLNLLTNAINASPAGGHIRLRSSSGNALWRVELEDQGPGVPDSER
                     EHIFDRFVRLKSAASPKLEGTGLGLAICRGIIELHRGRISAEAGSDGRGLRVVFEIPA
                     EWLPRASPVRSDAPEPVTSR"
BASE COUNT          677 a         1556 c         1557 g          820 t
ORIGIN      
        1 tctacgccac cgcggtcgtg gtcgcggttt ttctgccgga gctctttacc tcgagcgtcg
       61 aagggcgctt cgtgggaccg ctggcgctcg ccttcatatt tgtggtgctc gcctcgctcc
      121 tggtggcgat gacctccacc ccggcgctgt gcgcgctgct gctgcgccgg cacgacgagc
      181 ggccggagtc gcactggctg cagatactca agcgctggca gcgtttgtcg gtggccgccg
      241 tgggccggca tctgcgcatc gcaacgctgg tgctggccgc cctcgtcgtc atcgccctcg
      301 gcgcgctgcc ctttctcggc ggcaccttca tgccggactt ccgcgagggc cacttcgtga
      361 tgcaggtgtc gagctcgatc accggcacct cgctcgagca gatggcggag ctcggtaagc
      421 gggtgagcgc cgaggtactg gccgcgccat acgtccagac catcgagcag cagatcggac
      481 gcgccgagct gagcgaggac acgtggggtc cgcaccgcag cgagtttcac gtcgagctca
      541 agcccgacgc gccggtggat cagcaagccg cccaggagga gctgcgcggg attctcgcgc
      601 gctatccggg gattcagagc gaggtggtca ccttcctcgg cgaccgcatc agcgagagcc
      661 tgagcggcga gaccgcgcaa gtcgcgatca aggtgtgcgg cgacgacctc gacgttctcg
      721 atgccaccgg cgccaagatc gccgccgcgc tcgggaaagt gccgggcatc gtggatctgc
      781 agttcaaacg ccagagcggc acgccgacaa tcgccatcca gctcgtgcca caggcgcttg
      841 cggccgcggg gctcaaagcc caggacgtgc tcgacaccat cgagacggcc tacgcgggcg
      901 cgcaggtcgg gcagaccttc cacggcacgc gtacggtgga tgtcgacgtg ttgctgcccg
      961 agagctggcg gcgccagccg gcgctcgtcg gccggctcat gatcgcgagc cccttgggtc
     1021 cggtgccgct cgcccaggtc gcgcgcatcg acgtgaccca ggatcgctac agcatccagc
     1081 acgaaggcgg acagcggctg gtgacggtga cgttcaacgt ccgcggcgcc tccctccaga
     1141 gcgtcgtggc cgaggcccag cgaaccgtcg cctcatctgt tgcgcttctg ccgggcgtct
     1201 acctggaata cgccggcgcc gccgcggcgg agcggcagac ccgcaacgag ctgctgctct
     1261 actcggcgat cgccatggca ttgatcctga tgatcctctt cacctccttt cactggcggg
     1321 tcaatagctg gctggtcctg gccaacctgc ccttcagcct gatcggcagc atcatggcca
     1381 tcgccgtgac gggcgtcggg atctcgctcg gcaccgtcgt gggcctcgtc acggtgtttg
     1441 gtgtcagcgc gcgcaacgcg atcctgcagc tcgcgcacta cgagcacctg gtcgaagtcg
     1501 agggtgtttc ctggggcccg gcgctggtgc tgcgcggcgc caatgagcgg ctcgtcccaa
     1561 tcctcatgac cgccgcggtg accgcgctcg gtctcgcgcc gctcgccttc ggactgcacc
     1621 ggccaggcca ggagatcgag gggccgatgg cggtgacggt cctgggaggg ctcgtgtcgt
     1681 cgacgctgct gaatctcgtg gtgctaccgg cactggctga acgttacata cagggccatc
     1741 cgcgcaccga ccgtgagctg acggcgccgt ccgagggggc cgcatgagcc gcgcccatag
     1801 cgagtcgaca cgcagaccgg cggggttctg gcccgggggg tggtggcgcg tcatggagat
     1861 gcgcataggc gtcatcccgg taccggtgta cgtcacgctg ctcgccgcca tcgcctattt
     1921 cgtggccgcg ggcaagatcc cgaccgagat gaacatgatg atcgcgctgc tggcggtcgc
     1981 ggggttcacc tgcgccgagc tgggtcatcg cgtgccggtc atcaatcaga tcggaggcgc
     2041 cgcgatcttc gcgaccttcg tcccctcatt cctgaattat cggcattgga ttcccgctca
     2101 ggttgttgag gtggtgacgg acttcaccaa gtccaccaac ttcctctacc tcttcatcgc
     2161 ggcggtggtg gtgggcagca ttctcagcat gaaccgcggc gtgctgatcc gcggtttcct
     2221 gcggctgttc gtacccatgg ccgtcgcctc gctcgcggca ctgctcgtgg gcacggcggt
     2281 cggcaccgtg ctcggtctcg gcgcgcgcca taccctgctc atgatcgtga ttccggtgat
     2341 ggccggcggt gtgggcgagg gagcgatccc gctctcggtc ggctacggag cgctgtggca
     2401 ccagccgcag ggagagctgt ttgcgcaggt gctgccccca gtgatgttcg ggagcctcac
     2461 ggccatcctg ctggccggtg tcctgaactt cctgggtcgc cgctacccgc ggctcaccgg
     2521 ggaaggccgc ctgcaggctg aggagagcga gcagctcgcg acccaggagc acgaccggcc
     2581 ggtgaatctg gacgtcacgc acatcgccgc tgcgggactg ttggtggtgg cgctctacct
     2641 ggtcggtctc atctgcttcc agacattccg cctgccggcg cccgtggcga tgctgctgct
     2701 cgcggtactg gtcaagctcg cgcaagccgt gcctccgtcc ctgcagcagg gtgcgtacgt
     2761 cgtctacgag ttcttccgcc gcgccgtcac ttagccgctg ctgttcgcca tcggcgtcgc
     2821 cctgacgccg tgggagcggc tcatggcggc ctttgcgccg cgcaatctcc tcaccattgt
     2881 cgccacggtg gtgacgctgg tcgtcacggg cttcttcgtc gggcgctgga tccggctctt
     2941 ccccatcgac accgcgatcg tcatcgcctg ccgtgcgggg cagggtggca cgggcgatgt
     3001 ggcgatcctg acggccgcca accgcatgca gctgatgccc tttgcccaga tcgccacccg
     3061 catcggcggt gcgatcacca tcaccgtgac gttactggct ctggcgcatc tgacctgacg
     3121 ggggatgcgc gcggtagcca ctcagcgggt atctcgaaca ccactctcag gccgcgcccg
     3181 tcgctccccg cctcggcgct gatgcgcccg cggtgcagct cgatgatgcc gcggcagatc
     3241 gcgagtccga gcccggtgcc ttcgagcttg gggctggcgg cagatttcag gcgcacgaag
     3301 cggtcgaaaa tgtgctcgcg ctccgagtcc ggcacacccg ggccctggtc ctcgagctcg
     3361 acgcgccaca gtgcgttgcc tgacgaggag cgcaggcgga tgtggccgcc ggccggggag
     3421 gcattgatgg cgttggtgag gaggttcaga agcacccggc ggatccgccg cggctcgcag
     3481 acgggcaagc cgtcgccttc gtggctgtag gagaaatgca cgccgtggtg ctcggctagc
     3541 acctgggcgt cgacgctgaa attggcgagc agcgccgccg catcgtgcgg cgccagctgc
     3601 aatacgatgc tgcgcgactc ggcgcgcgac aggaacagca ggtcgtcgat ggtgccgttg
     3661 aggcgcagca gctcctcgag cagcgtctgt acgccatcct cgccctcgtg cccgagcgtg
     3721 ccgctggcga gcagcttctc cgcttgcagc cgcaccagac tcaggggcgt cttcagctcg
     3781 tgcgaggcgt cggcggcaaa gcggcgcacg ttcttgaacg acgcttccag acggtcgaac
     3841 attgcattca gcaggctcgc cagatccgcg atctcgtcct tgacctccgg aacggcgatg
     3901 cgctcgctca ggttctccga accgatgcgg ctcgcggtgg cgcgtatcag ccgcaccggc
     3961 cgcagcgcga gccggctcaa ggcgagcccg atcgcgatgc tgacagccag catcagcgcc
     4021 agcagcgccg cgccgacttc cgcgtagccc tccatcactt cgccgacgtg cgtcgccggc
     4081 gtcgcgatcg tcacgtcgta gggcggaaga atgaactcgg cgacccgcag cgggccgcgc
     4141 tcccaggaca catcgtagcg atgcgctccc ttgacgtccg gcagggcgcg gccgttgaga
     4201 ttgctcgagt agaagatggt gccggtgccg tgccggtgga tgtcgatgta gaacagcgtc
     4261 gcggcggctt cggtagtgtc gcgaatgcgc tgctcgatct gcgcgctgct gagtacgctg
     4321 acgtcaggac cgaggcgcgc cttgagctcc tggtactccg actgattgag caaatcgagc
     4381 ccgtggatca gatagccacg cagcaggtag taaccgacga cgaacaggca cgcgagcgtc
     4441 accgtgctgg cggctgcgta ccacagagcg agtcttgcgc cgatcgactt cactgcaact
     4501 tgtagcccac gccgcgcacg gtcttgaaga gcggcttttc gccgccgcta tgcagtcgca
     4561 ggcgcagctt gctcatgtac acgtccagca ggttggcgtc gactccgtcg
//
LOCUS       DATLKL010000809         4200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_167793, whole genome shotgun sequence.
ACCESSION   DATLKL010000809 DATLKL010000000
VERSION     DATLKL010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167793"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VM713_10985"
     CDS             <1..203
                     /locus_tag="VM713_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HVH84827.1"
                     /translation="HGFLALADETHVQYKVTDFWVREAERTLKWNDAALCIRWPLPAG
                     VEPLLSAKDRDGTPLARAECFP"
     gene            200..1078
                     /gene="rfbD"
                     /locus_tag="VM713_10990"
     CDS             200..1078
                     /gene="rfbD"
                     /locus_tag="VM713_10990"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HVH84828.1"
                     /translation="MRVLITGSSGQLGRALAAAAPPGDAVRVCAHAELDIADEAAVAA
                     LCRDFGPELVINAAAFTRVDEAERSAEAAARANALGPAVLAAACRAAGAWLTQVSTDF
                     VFDGRQGRPYDVAAQPNPLSVYGRTKLEGEAAVRRELGERCAIVRASWLYSATGGFAA
                     RMLALMKSRPELRVVSDQVGSPTHAAGLAGVLWALSARRAAGLWHWCDSGVASWYDFA
                     VATAEEALRSGLLRTAPPIVPIGTADYPTAAARPAYSVLDKRLTEQVLGITAPHWRTA
                     LRATLAELSSAAGARP"
     gene            1075..2187
                     /gene="rfbB"
                     /locus_tag="VM713_10995"
     CDS             1075..2187
                     /gene="rfbB"
                     /locus_tag="VM713_10995"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HVH84829.1"
                     /translation="MRRLLVTGGAGFIGANFCHYWCAAHPADRLVVLDALTYAGNVAS
                     IAALIDTGRLRFVRGDIGERERVAALLAEESLGIIVNFAAESHVDRSLEDPGQFLRTN
                     VLGTQALLEAARSAWGREAGPRGCRFHHVSTDEVYGSLAPAAAAFTESTPYAPNSPYA
                     ASKAAADHLVRAYAHSFGLPVTVSNCSNNYGPFQFPEKLLPLCILNILEGRRLPIYGD
                     GLQIRDWLHVSDHCRAIELILEKSAAGETWNIGGSTQEPNLHVVRAVCDLLDQAFAAE
                     PALAARFPRCPPALGSSARTLIEHVRDRPGHDRRYAVDSDKIARRLGFRAATALGAGL
                     ATTVRWYLDHEAWWRGVVSGEYRQWYARQYPQAERT"
     gene            2184..3044
                     /locus_tag="VM713_11000"
     CDS             2184..3044
                     /locus_tag="VM713_11000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVH84830.1"
                     /translation="MTADLTPASDDAQRAARALFRRARLQRWLGRRSEALALCRAAVE
                     RADYSRAYTLLSQLSLPGEDYFRVLARIHRHLRPRTYLEIGVSRGDSLACVLPATQVL
                     GIDPAPNLAKPPPANVRIYRETSDDFFARYSPRAELGSAAVELAFIDGMHHFEYALRD
                     FINVERTCERGSVILMHDCYPLDERTAQREQVTGFWSGDIWRLLLLLRERRPDLTVRT
                     IATPPTGLGIVLNPDPSSRALAERRDELIAEYLARDFSVLAAAKPEALGLIENRWPVI
                     RALLDSRPRT"
     gene            complement(3075..3932)
                     /locus_tag="VM713_11005"
     CDS             complement(3075..3932)
                     /locus_tag="VM713_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84831.1"
                     /translation="MTAPPARRPFLVFVRAGADSLHGRLISEDPQRNWDCCVSWYVTP
                     RSEALAEYYSHGGFNKLDGFVEFARERPEYRDYRYLMLLDDDVYLRPGDLSRFFTLCE
                     RYATYLSQPALRWFTHTTINALVRNPVCVLRRVTFVEVMAPCFSQAALAELLPTFGMT
                     RSTWGNDLAWAGTIGARHPIYVVDAVGMDHTRTGDGRPTAFYRKLAEMGVDPGAELAQ
                     VRRMYPAFRGSRTLPSGHVFRAGVPAWAAPALMRLIEHLKFIVRIRKKTLLHLRAWRA
                     RFADLVRAR"
     gene            complement(3940..>4200)
                     /locus_tag="VM713_11010"
     CDS             complement(3940..>4200)
                     /locus_tag="VM713_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84832.1"
                     /translation="ASAIGDITGDLATKRARISGSDTLPGQRATLQALVPLAEISEYQ
                     SRLKALTGCQGAYTMELSHYDPVPPRKQQELAQAWRPREAQE"
BASE COUNT          593 a         1512 c         1465 g          630 t
ORIGIN      
        1 cgcacgggtt tctcgcgctg gccgacgaga cgcacgtgca gtacaaggtc accgacttct
       61 gggtgcgcga ggcggagcgc accctcaagt ggaacgatgc cgcgctctgc atccgctggc
      121 cgctgcccgc gggggtcgag ccgctcctgt cggcgaagga tcgtgacggc acgccgctcg
      181 cccgtgcgga gtgctttccg tgagagtgct catcacgggc tcttcgggcc agctcggtcg
      241 cgcgctcgca gccgcggctc ccccgggcga tgcggtgaga gtctgcgccc acgccgagct
      301 cgacatcgca gatgaggccg ccgtggcggc cctgtgccgc gacttcggac cagagctcgt
      361 catcaatgcc gccgccttca cccgggtcga tgaggccgag cgctccgctg aggccgccgc
      421 ccgcgccaac gccctgggac ctgcggtgct ggccgcggcc tgccgcgccg cgggtgcctg
      481 gctcacgcag gtgtcgacgg acttcgtgtt cgacggccgg caggggcgac cttacgacgt
      541 cgcggcacag ccgaatcccc tttcggtcta cggccgcacc aagctcgagg gcgaggcggc
      601 ggtcaggcgc gagctggggg aacgctgcgc gatcgtgcgc gcctcgtggc tctacagcgc
      661 caccggcgga ttcgcggcac ggatgctcgc cctgatgaag agccgcccgg agctgcgggt
      721 ggtgagcgac caggtgggct cgcccacgca cgccgccggg ctcgccgggg tgctgtgggc
      781 gctgagcgcg cggcgcgccg cgggactgtg gcactggtgt gactcggggg tcgcgagctg
      841 gtacgacttt gcggtggcga cggccgagga ggcgctgcgc tcaggtctgc tgcgcactgc
      901 cccgccgatc gtcccgatcg gcaccgccga ctacccgacc gcggcggcgc gccccgccta
      961 cagcgtgctc gacaagcgcc tcaccgagca ggtgctcggc atcacagcgc cccattggcg
     1021 caccgcgctg cgcgcaacgc tcgcggagct gagcagcgcc gctggagcgc ggccgtgagg
     1081 cggctgctgg tcaccggggg agcgggtttc atcggcgcaa acttctgcca ttactggtgc
     1141 gccgcgcacc cggccgatcg cctggtcgtg ctcgatgccc tcacctacgc cggcaatgtg
     1201 gcaagcatcg ccgcgctcat cgacacgggg aggctgcggt tcgtgcgcgg cgacatcggc
     1261 gagcgcgagc gcgtcgcggc gctgctcgca gaagagtcgc tcggcatcat cgtgaacttc
     1321 gccgccgagt cgcacgtcga ccgctcgctc gaggacccgg ggcagttcct gcgcaccaac
     1381 gtgctcggca ctcaggcgct gctcgaggca gcgcgctcgg cctgggggcg cgaggccggc
     1441 ccgcgcggct gccgcttcca tcacgtatcg accgatgagg tctacggctc gctcgcgccc
     1501 gcggccgcgg ccttcaccga gagcacgccc tatgcgccca actccccgta cgccgccagc
     1561 aaggcggcgg ccgatcacct ggtgcgcgcc tacgcgcaca gctttggcct gccggtgacg
     1621 gtcagcaact gctcgaacaa ctacgggccg ttccagtttc cggagaaact gctgccgctg
     1681 tgcatcctca acatcctcga gggccgccgg ctcccgatct acggcgacgg cctgcagatc
     1741 cgcgactggc tgcacgtgag cgatcactgc cgcgcgatcg agctgatcct ggagaagtcc
     1801 gccgccggcg agacctggaa catcggcggc tcgacccagg agccgaatct tcacgtggta
     1861 cgcgcggtgt gcgaccttct cgaccaggcc ttcgcggccg agccggcact cgccgcgcgc
     1921 tttccgcgct gcccgccagc actggggagc tccgcgcgca ccctcatcga gcatgtccgt
     1981 gaccgacccg ggcatgaccg gcgctatgcg gtcgactcgg acaagatcgc ccgccggctc
     2041 ggtttccgcg ccgccactgc gcttggcgcc ggccttgcga ctacggtgcg ctggtatctc
     2101 gaccacgagg cctggtggcg cggcgtcgtg agcggcgagt atcggcagtg gtacgctcgg
     2161 cagtatccac aggcagagcg cacgtgaccg ccgacctcac tcccgcgtcc gacgacgccc
     2221 agcgcgccgc gcgcgcgctg ttccggcggg cgcgcctgca gcgctggctg gggcggcgga
     2281 gcgaggcgct ggcgctgtgc cgcgcggcgg tcgagcgcgc cgactattcg cgcgcctaca
     2341 cgctcctgtc gcagctgtcg ctcccgggcg aggattactt ccgcgtgctc gcgcggattc
     2401 accgccatct gcgcccgcgg acctatctcg agatcggcgt ctcgcgcggc gactcgctcg
     2461 cgtgtgtgct gcctgcgacg caggtgctcg ggatcgatcc cgcgccgaac ctcgccaagc
     2521 ccccgcccgc caacgtgcgg atctaccgcg agacgagcga cgatttcttc gcccggtact
     2581 cgccgcgtgc cgagctcggc agcgccgccg tcgagctcgc gttcatcgac ggcatgcacc
     2641 atttcgagta cgcgctgcgc gacttcatca acgtcgagcg cacctgcgag cgaggctcgg
     2701 tgatcctgat gcacgactgc tatccgctcg atgagcgcac ggcacagcgc gagcaggtca
     2761 cgggcttctg gagcggcgat atctggcggc tgctgctgct gctgcgcgag cggcgcccgg
     2821 atctcacggt gcgcaccatc gccaccccgc caaccgggct cggtattgtc ttgaatcccg
     2881 acccctcctc gcgcgcactc gccgagcggc gggacgagct gatcgcggaa tacctcgccc
     2941 gggacttcag cgtgctcgcc gcagctaagc ccgaggcgct tgggctcatc gagaaccgct
     3001 ggccggtgat ccgcgcgctc ctcgactcgc gcccgcgcac ctgaaagcgc gtcggcaccg
     3061 gacgtgcccg ataactaccg cgcccgcacc agatcggcga aacgcgcccg ccatgcgcgc
     3121 aggtgcagca gcgtcttctt gcggatgcgg acgatgaact tgaggtgctc gatgaggcgc
     3181 atgagcgccg gcgccgccca ggccggtacg ccggcgcgga agacatgacc ggacggcagc
     3241 gtccgcgagc cgcggaaggc gggatacatg cgccgcacct gtgcgagctc ggcgcccgga
     3301 tctacgccca tctcggccag cttgcggtag aaggccgtgg ggcgcccgtc gccggtgcgg
     3361 gtgtggtcca tgccgaccgc gtccaccacg tagatcggat gtcgtgcgcc gatcgttccg
     3421 gcccaggcga ggtcgtttcc ccaggtgctg cgcgtcatgc cgaaggtcgg cagaagctca
     3481 gcgagcgccg cctgtgagaa gcacggcgcc atgacctcga cgaaggtcac ccgccgcagc
     3541 acgcacacgg ggttgcggac cagcgcgttg atggtcgtgt gcgtgaacca gcgcagcgcc
     3601 ggctgcgaga gataggtcgc gtagcgctcg cacagcgtga agaagcgcga cagatcgccg
     3661 ggccgcaggt acacatcgtc atcgagcagc atcaggtagc ggtagtcgcg atactccggc
     3721 cgctcgcgcg cgaactcgac gaagccgtcg agcttgttga agccgccgtg ggagtagtat
     3781 tccgcgagcg cctcgctgcg cggcgtcaca taccaggaaa cgcagcagtc ccagttgcgc
     3841 tgtggatcct cgctgatcag gcgcccgtgg agcgaatcgg cgccggcgcg cacgaatacc
     3901 aggaagggcc gccgcgcggg cggcgccgtc acgcccacct tattcctgcg cctcccgcgg
     3961 ccgccaggcc tgcgccagct cctgctgctt gcgcggcggc accgggtcgt agtggctcag
     4021 ctccatggtg taggcgccct gacatccggt aagcgccttg agccgcgact ggtactcgga
     4081 gatttccgcg agcggcacca gagcctgcag cgtggcgcgc tgacccggga gcgtgtcgct
     4141 gccggagatg cgcgcgcgct tggtggcgag atccccggtg atgtcgccga tggcgctcgc
//
LOCUS       DATLKL010000810         1766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_100180, whole genome shotgun sequence.
ACCESSION   DATLKL010000810 DATLKL010000000
VERSION     DATLKL010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100180"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VM713_11015"
     CDS             <1..599
                     /locus_tag="VM713_11015"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HVH84833.1"
                     /translation="IVTEEDHFVHVSGHPARDELVRMYQMVRPRVAIPVHGEARHLIA
                     HAHLAEECQVQQPLVIQNGDMVRLTPSGAAIVDEVPVGRLASDGKGLLPIDGSALKDR
                     RRVTFNGSVVATLVVDRQGRLVASPAVSLIGLVEEPAADAAEPFLRDSLKRTLDELPA
                     GARRDDQVVRDAARGALRRVINERFGKRPLIEIHLVRI"
     gene            751..>1766
                     /locus_tag="VM713_11020"
     CDS             751..>1766
                     /locus_tag="VM713_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HVH84834.1"
                     /translation="MAATSAPPSAPDITTVTIEDEMKRSYLDYAMSVIVARALPDVRD
                     GLKPVHRRILYSMKVNGNEWNRGYRKSARIVGDVIGKYHPHGELAIYDAMVRMAQGFS
                     MRLPLVDGQGNFGSMDGDPPAAMRYTEARLARAAEALLEDIDRETVEFQPNYDDTARE
                     PGVLPAQWPNLLVNGAGGIAVGMATNIPPHNLGEVIDACLAYVDRPDISVDQLIELMP
                     GPDFPTGGIIMGRAAIRQAYHTGRGSLVMRGRAAIEEIAKGREAIVITEVPYQVNKAR
                     MIERIAEMVQEKIIDGVADLRDESDRHGVRVVVELKRDALPDLVLNQLYHHTPLQSTF
                     GVNML"
BASE COUNT          316 a          584 c          568 g          298 t
ORIGIN      
        1 agatcgtcac cgaagaggat catttcgtcc acgtctccgg acatccggct cgcgacgagc
       61 tggtgcgcat gtatcagatg gtccgtccgc gagtggcgat tccggttcac ggcgaagctc
      121 gtcatctgat tgctcatgcc catctcgccg aggaatgtca ggtgcagcag ccgctcgtga
      181 ttcagaacgg cgacatggtc cggttgaccc cgagcggagc tgcgattgtc gacgaggtcc
      241 cggttggccg cctggcctct gacggcaaag gactgctgcc gatcgacggc agcgcgctga
      301 aggacaggcg ccgggtaacg ttcaatggaa gcgtggtggc tactcttgtg gtcgatcgtc
      361 aaggccggct ggtggcctca ccggcagtca gcctgattgg gctggtcgag gaaccggccg
      421 ccgatgccgc cgagccgttt cttcgtgaca gcctcaagcg cacgctcgac gagctaccgg
      481 ccggcgcccg ccgtgacgat caggttgtgc gcgacgcggc gcgcggggca ttgcggcggg
      541 tcatcaacga gcgcttcggc aagcggccgc tgatcgaaat ccatctcgtt cggatctgac
      601 gatcgaacgc gcggcaaatc ccgtttgttc gcacctgcga aatagggtaa tctgctcgcg
      661 aattcctaca tttagcttcg cagcggtgtc tcgtggcaca gccgcgattt ttgcgagacc
      721 gcgagccttt ccagcacagt cgagcagatc ttggccgcca cttccgcgcc cccttccgcc
      781 cccgacatca ccaccgtcac gatagaagac gagatgaagc gctcgtacct cgattacgcg
      841 atgagcgtga tcgtggcgcg ggcgctcccc gatgtgcgag acgggctgaa acccgtccac
      901 cgtcgcatcc tttactcgat gaaggtcaac ggcaacgagt ggaaccgcgg ctaccgcaaa
      961 tcggcccgca tcgtcggcga tgtgatcggg aaataccacc ctcacggcga gctggcgatc
     1021 tacgacgcca tggtgcggat ggcgcagggc ttttcgatgc gcctgccgct ggtggacggg
     1081 cagggcaatt tcggctcgat ggacggcgac ccgccggcag cgatgcgcta caccgaggcg
     1141 cgtctcgcca gggcggccga ggcgctgctg gaggacatcg atcgcgagac cgtcgagttc
     1201 cagcccaact acgacgacac cgcgcgggag cccggggtgc tgccggccca gtggccgaac
     1261 ctgctggtga acggcgcggg cggcatcgcg gtcgggatgg cgaccaacat cccgccccac
     1321 aatctcggcg aggtgatcga cgcctgcctc gcctatgtcg accgccccga catctcggtc
     1381 gaccagctca tcgagctcat gccgggaccg gacttcccga ccggcggcat catcatgggc
     1441 cgggccgcca ttcgccaggc ctaccacacc gggcgcggct cgctggtgat gcgcgggcgc
     1501 gccgccatcg aggagatcgc caaggggcgc gaggccatcg tcatcaccga ggtgccttac
     1561 caggtcaaca aggcgcgcat gatcgagcgc atcgccgaga tggtccagga aaagattatc
     1621 gatggcgtcg ccgacctgcg cgacgagagc gaccgccacg gcgtccgcgt cgtcgtcgag
     1681 ctcaagcgcg acgccctgcc agacctcgtg ctcaaccagc tctaccatca tacgccgctg
     1741 caatcgacct tcggcgtcaa catgct
//
LOCUS       DATLKL010000811         1689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_190402, whole genome shotgun sequence.
ACCESSION   DATLKL010000811 DATLKL010000000
VERSION     DATLKL010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190402"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /gene="gspF"
                     /locus_tag="VM713_11025"
     CDS             <1..1069
                     /gene="gspF"
                     /locus_tag="VM713_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HVH84835.1"
                     /translation="AKAQRSFSLIRRVSPTDLALFTRQLATLVRAGLPLEESLLAVSQ
                     QTEKPRVQSIVLGVRARVMEGHTLADGFAEFPRVFPEIYRATVAAGEQSGHLDNVLER
                     LAEYTESREQIRQKILAALLYPIVLTVMCFTIVSGLLVFVVPKVVAVFESAKGKLPLI
                     TRALIATSDFLRQYGLYLLIALAVAVWLFRRWQRDPAAKRRLQRFLLTVPLVGKLSRG
                     FNTARFTRTFSILSASAVPVLEALRIAGEVVTNLPMRDAVADAAARVREGAPIGRSLA
                     VSRMFPPMTIHLISSGESSGELESMLERAAISQERELDALLAAMVGLLGPLLIILMGL
                     FVMGIVFAMLLPIFEMNNLIH"
     gene            1217..1486
                     /locus_tag="VM713_11030"
     CDS             1217..1486
                     /locus_tag="VM713_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84836.1"
                     /translation="MSEKPESDEFEDEEADEPVIGDGDVDLDQVIKDLDLAKRRGQKA
                     GDPAWRRLERLLEDKHTAELTSDFEDYEIGEDGESPRKGRRKPRN"
BASE COUNT          265 a          591 c          554 g          279 t
ORIGIN      
        1 ggcgaaggcc cagcgttcct tcagcctgat ccggcgggtc tcgccgaccg acctcgccct
       61 cttcacccgc cagctcgcca cgctggtgcg cgccgggctg ccgctcgagg aatcgctgct
      121 cgcggtctcg cagcagaccg agaagccgcg cgtgcagagc atcgtgctcg gcgtgcgtgc
      181 gcgcgtcatg gagggccaca cgctcgccga cggctttgcg gaattccccc gtgtattccc
      241 cgagatctac cgcgccaccg tggcggccgg cgagcagtcc ggccatctcg acaacgtgct
      301 cgagcggctc gccgagtaca ccgagagccg cgagcagatc cgccagaaga tcctggcggc
      361 gctgctgtat ccgatcgttc tcacggtcat gtgctttacg atcgtctcgg gcctgctggt
      421 attcgtggtg cccaaggtgg tcgcggtatt cgagtccgcc aagggcaagc tccccctgat
      481 cacccgggcg ctgatcgcca ccagcgactt cctgaggcaa tacgggctct atctgctgat
      541 cgcactggcg gtggcggtct ggttgttccg ccgctggcag cgcgatccgg ccgccaagcg
      601 ccgcctacag cgtttcctcc tcacggtgcc gctcgtcggc aagctctccc gtggcttcaa
      661 caccgcgcgc ttcacccgca ctttcagcat tctgtcggcg agcgcggtgc cggtgctgga
      721 ggcgctgcgt atcgccggcg aggtggtcac caatctgccg atgcgggacg cggtagcgga
      781 tgctgcggcg cgtgtgcgcg agggcgcgcc gatcgggcgc tcgctggcgg tgagtcgcat
      841 gtttccgccg atgacgatcc acctgatctc gagcggcgaa tcgagcgggg agctcgagag
      901 catgctggag cgggccgcca tcagccagga gcgcgagctc gatgccctgc tcgccgccat
      961 ggtgggcctg ctcgggccgt tactcatcat tctgatggga ctgttcgtca tgggcatcgt
     1021 ttttgcaatg ctgctgccca tcttcgagat gaataacctc atccactgac gctgcgccgc
     1081 gggtccgagg gcccgccggc ggtctgcggg gcctgattac gggcacggtt cacaaattcc
     1141 gcacgcgtga cattgcggat gcaatgggat ctgatttaag ttcgcgccgt ccaattcccg
     1201 ccgacagccg tcacgcatga gcgagaagcc ggaatcagac gaattcgagg acgaagaagc
     1261 cgacgagccc gtcatcggcg acggtgacgt cgacctcgat caggtcatca aggacctcga
     1321 cctcgccaag cgccgcggcc agaaggccgg agacccggcg tggcggcgcc tcgagcggct
     1381 gctcgaggac aagcacaccg ccgagctcac cagcgacttc gaggattacg agatcggcga
     1441 ggacggcgag agtccccgca agggccggcg caagccccgc aactgactcc ccggcccgcc
     1501 cggcaacggc tcctcagccc ctcgcggccg cgcgcgcagc gtggcttgcg gcggcagccc
     1561 ccgcgtccac gatgccccct ggcgctccac cgacgcgcaa tcgctcgagg aacgcgccgg
     1621 gcgtcagcgc ccggctcagc aggtacccct gtacgaggtc acacccggcg tcgcgcagga
     1681 actcgagct
//
LOCUS       DATLKL010000812         1682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_134025, whole genome shotgun sequence.
ACCESSION   DATLKL010000812 DATLKL010000000
VERSION     DATLKL010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134025"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..874
                     /gene="pdxH"
                     /locus_tag="VM713_11035"
     CDS             182..874
                     /gene="pdxH"
                     /locus_tag="VM713_11035"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HVH84837.1"
                     /translation="MTEHHELLPEPLPAEPLAVVARWLADAWAARRQPNADSMVLATS
                     TPDGRPSARVVLCRDIVPQPGYVVFYTNYQSSKGRQLEDNPRAAAVMHWDALARQVRL
                     EGAVVASPAAESDAYFSSRSWDKRIGAWASAQSEPLPSRAALERAVAAAAQRFGAPIP
                     GSPGADENLKIDVPRPPHWGGYRLWVDAVELWVEGSQGRIHDRARWTRSLRPAPGGAF
                     AAGAWQVTRLQP"
     gene            879..1652
                     /locus_tag="VM713_11040"
     CDS             879..1652
                     /locus_tag="VM713_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84838.1"
                     /translation="MWRNIRILALLLVLAAVALEQWLERVHTQSWRETLWVGIYPLNA
                     DGARATQSYIETLTRADFIAIESFFAREAHRYGVQLDDPVHVELYPEGRELPPELAPG
                     AGPLGIAWWSLKLRWFAAHASTVPGRVPSRIRLFVLYHDPTTLERAPDSHGMQKGLVG
                     VVHAFAAGTMSGANDIVIAHELLHTVGASDKYDLTTGEPLKPIGYGDPGQLPLYPQAE
                     AEIMAVRRALSPQESEMPRSLRSEVVGAATALEIRWTHP"
BASE COUNT          246 a          596 c          614 g          226 t
ORIGIN      
        1 gctggcgtcg cgagcagcga gcgcggctgc ggcgtgagcg gctcgtcgcg ccagttgatg
       61 acgaagagca gcgcgagcag cagcagcgcg atgagcgcga ggctccccgc cgcgagcccg
      121 acccacttga ggatgcggcc cgcgatgcgc gagagcggta tcatggggtc gagcctacca
      181 cgtgaccgaa caccacgaac tcctgcccga gccgttgcca gccgagccgc tggcggtcgt
      241 cgcgcgctgg ctcgcggacg cttgggcggc gcgccgccag ccgaacgccg actcgatggt
      301 gctcgccacc tcgaccccgg acggacgccc gtcggcgcgc gtagtgctct gccgggacat
      361 cgtgccgcag ccgggatatg tcgtcttcta caccaactat caatcgagca aggggcggca
      421 gctcgaggac aacccgcgtg cggcggcggt gatgcactgg gatgcgctcg cccgacaggt
      481 gcgcctcgag ggcgcggtgg tggcgagccc tgcggccgag agtgatgcct acttctcctc
      541 gcgctcctgg gacaaacgca tcggcgcctg ggcgagcgcg cagagtgagc ccctgccctc
      601 gcgcgcggcg ctcgagcgcg cggtggccgc cgccgcccag cgcttcggcg cgccgatccc
      661 agggagcccc ggcgccgacg agaacctgaa gatcgacgtg ccccgcccgc cccactgggg
      721 aggctaccgc ctgtgggtgg atgcggtgga gctgtgggtc gagggcagtc agggccgcat
      781 ccacgaccgc gcgcgctgga cgcgctcgct gcggccggcg cccggcggcg ccttcgcggc
      841 gggcgcgtgg caggtcacgc ggctgcagcc ctgatgcgat gtggagaaac atccgcatcc
      901 tcgcgctcct gctggtgctc gccgcggtgg cgctcgagca gtggctcgag cgcgtccaca
      961 cccagagctg gcgcgagacg ctctgggtgg ggatctaccc gctcaacgcc gacggcgcac
     1021 gcgcgacgca gagctacatc gagacattga cgcgcgcgga cttcattgcc atcgagagct
     1081 tcttcgcgcg cgaggcgcac cgctacggcg tgcagctcga tgatcccgtg cacgtcgagc
     1141 tctatcccga ggggcgcgag ttgccgccgg agctggcgcc cggcgcgggt cccctcggga
     1201 tcgcctggtg gagcctcaag ctccgctggt tcgccgcgca cgcgagcacc gtgccggggc
     1261 gcgtgccgtc gcgcatccgc ctcttcgtcc tctaccacga ccccacgact ctcgagcgcg
     1321 cacccgactc gcacgggatg cagaaggggc tggtcggcgt cgtgcatgcc ttcgcggcgg
     1381 gcacgatgag cggcgccaac gacatcgtga tcgcgcacga gctcctgcac acggttggcg
     1441 cgagcgacaa gtacgatctc acgaccggcg agcccctcaa accgatcggc tacggtgatc
     1501 ccgggcagct gccgctctac ccgcaggccg aggcggagat catggcggtg cgccgcgccc
     1561 tgagcccgca ggaatcggag atgccgcgca gcctgaggag tgaggtggtg ggtgcggcaa
     1621 ccgcgctgga gatccgttgg acgcatccct gagcggcggc aacggcgcgg cggtgcacag
     1681 cg
//
LOCUS       DATLKL010000813         4673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_156892, whole genome shotgun sequence.
ACCESSION   DATLKL010000813 DATLKL010000000
VERSION     DATLKL010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156892"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2244)
                     /locus_tag="VM713_11045"
     CDS             complement(<1..2244)
                     /locus_tag="VM713_11045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH84839.1"
                     /translation="MLFVAYTLLAGGSLLYVEHVSARLTESFFPLPWLADGVAISLML
                     AWGRRTWPGVFLGSIFVWGVLRGDPAIPVGVDAIGETLSVVIAVAILRAWHFRRQLDR
                     LADPVILLGAALAGRAVAALADIGGVLAGAWLTPHSLPPEFLRLATRPGTATPAVTPQ
                     LVWAIARWQVIALTGIALTVPALLASPRKLRHALRARPLKLARLGVFSLVWVSCALRL
                     SATWTCWPLLLAAVMLVAWAATEFGALAAALCTLLFACTAGAALCHGVGPLATTDFLG
                     GLAATWGFIGLLCCVSPVLTVVLTARQQHDRRLAVLANRYRSLFEANPTPAWVADASS
                     GTILMANAEAIRRYGYAESEFLRMKIPELCADRSRDSQPPPPDADLIAAPLAKHLTRD
                     GRVIDVELVCTPLEPEGRAVNLVHAVDMTDQQELRRRLLSTVDRESFRVAQELHDGLG
                     QVLAGLAIGSEALLQRTAGAAALDASGTAQVSELASHARHAETHLFQLTSGVSPLDDL
                     EGNLLEALQRLPSTLPAAERARVEVIVEGEAPVMLSLEGREHLYRVVQESLANALKHA
                     RADRIVIRAAIDHERIRVSVEDNGVGLKEERAGPGLGLQSMRLRASAVGAELAIETLA
                     GAGTVVTCSCPQAEGLGGLAAADSRTPKWTAPTGAAGDRRDFSARAYLAVSALVVAAC
                     WLGGAISHALASAHNSGFTYADASLAVPSLLSGVVVAALLQGGRRYWPSVLLGMLLVR
                     IGQIGEPL"
     gene            complement(2480..2962)
                     /locus_tag="VM713_11050"
     CDS             complement(2480..2962)
                     /locus_tag="VM713_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVH84840.1"
                     /translation="MSLVAALALAGGSALGVATDATPVDTAAFVRLTPEQIHWQDMPE
                     AYGGQIATIAGDSNGHGLYVQRVRFPPHVMDRPHWHPGDRYVTVIKGTWYTGTGATFD
                     PAQAVPLKPGSYMFHPARALHWDGSNSDEEVIVQVVGLGPVETSAADPSKPFWVKVRP
                     "
     gene            complement(2978..4162)
                     /locus_tag="VM713_11055"
     CDS             complement(2978..4162)
                     /locus_tag="VM713_11055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH84841.1"
                     /translation="MPVSIERRQFLVATGATLLGVPLGGCSFGLRGSSRSAAGPVLEL
                     PPLRASPDRVTAITVCTRPFRAQGPRLDVEQIQGKTVVHNYGHGGSGWSLSWGSGEIA
                     VTKAMATGEREVAVIGCGAIGLTTALLLQRAGAGVTIYTRELPPDTRSALATGVFTPD
                     SRICFEQYATPDFRQLWEVMARRSFATYQSFLGLPGTPVEFIDSYFVSDTAEPTRRSL
                     PDDDPRPRFAELQRELLGDLLPRTQLYAPGTHPFGARHVRGGAQLMFNLAAYQRMLMS
                     DFAERGGKIRIDEFHTPADFARVREKVLVNATGYGARALLGDQSLVPVRGQLARMIPQ
                     PDIHYGLIYRGVAFVPRRDGLVFQVIGDSDYYGFDDASTVPDRPEAELAVQRIAGLFN
                     AT"
     gene            complement(4184..>4673)
                     /locus_tag="VM713_11060"
     CDS             complement(4184..>4673)
                     /locus_tag="VM713_11060"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HVH84842.1"
                     /translation="YDDLMDAGRALDLRLFGGRALSSLRLEKGYGSFQKDFRPDYTAA
                     ESGLDRFVDFTKPDFVGRTATLAERAAGPKRRFVVMEVAALDAEVIGYESIIRDGAAV
                     GYVTSGAFGHCVGKSLAAGYVPTALAREGARFEIDILGEMRAATVRLEPLYDPQGLKL
                     RS"
BASE COUNT          815 a         1573 c         1629 g          656 t
ORIGIN      
        1 cagcggctcg cctatttgcc cgatgcgaac cagcagcatt ccgagcaaca cggacggcca
       61 gtagcgccgg cctccctgca gcagggccgc gaccacgacg ccgctcagga gcgaaggaac
      121 cgccaggctt gcatcggcgt aagtgaatcc ggagttatgc gcgctggcca gcgcgtgtga
      181 gattgcgcca ccgagccagc acgccgcgac aacgagcgcc gagaccgcga gataagcgcg
      241 ggctgaaaaa tcgcggcgat cgcccgcggc ccccgtgggc gccgtccact tcggtgtgcg
      301 tgagtcggcg gccgcgagcc cccccaggcc ttcggcctgg ggacagctgc acgtgaccac
      361 ggttcccgcg cctgccagtg tctcgatggc gagctcggcg cccacggcgc tggcacgcag
      421 cctcatcgac tgcaggccaa gacccgggcc ggcgcgctcc tctttcaggc ccacgccgtt
      481 gtcctcgacg gagacgcgaa ttctttcgtg atcgatggcg gcgcgaatca cgatgcggtc
      541 cgcgcgcgcg tgcttcaagg cgttcgcgag cgattcctgg acgacgcggt acaagtgctc
      601 gcgcccctcg agcgagagca tcacgggtgc ctctccctcg acgatcacct cgacacgggc
      661 gcgctcggcg gccggcaggg tcgagggcag gcgttggagc gcctcgagga ggttgccctc
      721 gagatcatcg agcggcgaga cgccgctcgt cagctgaaac agatgggtct ccgcgtgtcg
      781 ggcgtggctg gccagttcgg atacctgagc cgtaccggat gcatcgagcg ctgctgcgcc
      841 ggcggtgcgc tgcagcagcg cctccgatcc gatcgcgagc cccgcaagaa cctggccgag
      901 accatcgtgc agctcctgtg cgacgcggaa ggactcccga tccaccgttg acagcagccg
      961 ccgtcgcagc tcctgctgat cggtcatgtc gacggcatgc accaggttga ccgcgcgtcc
     1021 ttcgggctcg agcggcgtgc acaccagctc gacgtcgatc accctgccat cgcgcgtcag
     1081 gtgcttggcg agcggggcag cgatgagatc ggcatcgggc ggcgggggct gcgaatcgcg
     1141 cgaccgatcc gcgcagagct ccgggatttt catgcgcaga aactcggatt cggcgtagcc
     1201 atagcgccgg atggcctctg cgttggccat caggatcgtc cccgagctgg cgtcggccac
     1261 ccaggcagga gtcgggtttg cctcgaacag ggatcggtag cgattcgcca gcacggcgag
     1321 tcggcggtcg tgctgttgcc tcgcggtaag caccaccgtg agcaccgggc tcacgcagca
     1381 gagcagcccg ataaagcccc aggtggcggc gagccctccg aggaaatcag tcgtcgcgag
     1441 gggtcccact ccgtggcaca gggctgcccc ggcggtgcag gcgaacagca gcgtacagag
     1501 cgccgcggcc agcgctccga actcggtggc cgcccacgcg accagcatca ccgccgcgag
     1561 gagcagcggc caacaggtcc aggttgccga gagccgcagc gcacagctca cccacaccag
     1621 cgagaacacg ccgaggcggg cgagcttgag gggacgcgca cgcagcgcgt gccgcagctt
     1681 cctcggactt gcgagcaggg ctggaacggt cagcgcgatg ccggtcaagg caatgacctg
     1741 ccagcgggca atcgcccaga cgagctgtgg agtcaccgcg ggggtcgcgg ttcccggtcg
     1801 ggtggcaagg cgcaagaact cgggtggaag gctgtggggc gtgagccagg cgccggcgag
     1861 tacgccccca atatcggcga gggccgcgac cgcacggccg gcgagtgcag ccccgaggag
     1921 aatgaccggg tcggcgagac ggtcgagctg ccggcggaag tgccaggcgc gcaggatcgc
     1981 cacggcgatg acgacgctca gcgtctcgcc gattgcgtcc acgcccaccg ggatggcggg
     2041 atccccgcgc aggacgcccc agacgaagat cgagccaagg aagactccag gccaggtgcg
     2101 ccggccccaa gcaagcatga ggctgatcgc cacaccgtcg gccagccagg ggagggggaa
     2161 gaaggactcg gtgagacgcg cgctcacgtg ctcgacgtaa agcaacgacc cgcccgcgag
     2221 cagggtgtac gcgacgaaaa gcaccagggc agccgcccac gaaggcaggg gatgcggcag
     2281 gagcctcagc cttccgatgg catacaacaa cgcgacaatt ctccggcggc cgtgtgttcc
     2341 ccggcctgaa ggctacaagt tgggtgccag tctcccacgg gcccggcacc cgattccgag
     2401 atcccggtgg cagaccacat aggcccgttg cagcgcaaca taacgccgcg tcccagcggc
     2461 gttcccgtcg gcgggccgct cagggtctca ccttcaccca gaagggcttg gagggatcgg
     2521 ccgcggaggt ctcgaccggc ccgagcccga ccacctgaac gatgacctct tcatcgctgt
     2581 tcgagccgtc ccagtgaagc gcccgagccg gatgaaacat gtagctgccg ggtttgaggg
     2641 gcacggcctg cgcgggatcg aaggtggccc cggtaccggt gtaccaggtt cccttgatca
     2701 ccgtgacgta gcgatcgccc ggatgccagt gaggccgatc catcacgtgc ggaggaaatc
     2761 tgacgcgctg cacgtagagc ccgtgaccat tcgagtcacc ggcgatggtc gcgatctgac
     2821 cgccataggc ttcgggcatg tcctgccagt gaatctgctc gggggtgagg cggacgaagg
     2881 ccgcagtatc gaccggtgtc gcgtccgtcg cgacgccgag cgcgctcccg cccgccagag
     2941 caagggctgc gactaagctc attctcatag ctgcttctca ggttgcattg aagaggccgg
     3001 caattctctg cactgctagc tcggcctccg ggcgatcggg aaccgtgctg gcatcgtcaa
     3061 agccgtagta gtcgctgtcg ccgatcacct ggaagacgag gccgtcgcgg cgcggcacga
     3121 acgcgacccc acggtagatc agaccgtagt ggatgtcggg ctgcgggatc atgcgtgcga
     3181 gctgtccgcg caccggcacg agcgactgat caccgagcag cgcccgcgcg ccgtagcccg
     3241 tcgcattcac gagcaccttc tcgcgcaccc gcgcgaaatc cgccggcgtg tgaaactcat
     3301 cgatgcggat ttttccgccg cgctcggcga agtccgacat cagcatgcgt tggtacgccg
     3361 ccagattgaa catgagctgc gcgccgccgc gcacgtggcg tgcgccgaag gggtgggtac
     3421 cgggcgcata gagctgcgta cgcggcagca gatcgccgag gagctcacgc tgcagctcag
     3481 cgaacctcgg gcgcggatcg tcgtcaggga gcgagcggcg cgtcggctcg gccgtatcgc
     3541 ttacgaagta gctgtcgatg aactcgacgg gagtgcccgg caggccgagg aagctctggt
     3601 aggttgcaaa ggaacggcgc gccataacct cccagagctg gcggaagtcg ggagtcgcat
     3661 actgctcgaa gcagatgcgc gagtcgggcg tgaacacgcc ggtggcgagc gcggagcgcg
     3721 tgtcgggcgg cagctctctc gtatagatcg tgacgcctgc gccggcgcgc tgcagcagca
     3781 gcgccgtcgt cagcccgatc gcgccgcagc cgatcacggc gacttcgcgc tcgccggtcg
     3841 ccatcgcctt cgtgaccgcg atctcgcccg atccccagga gagggaccag ccgctgccgc
     3901 cgtggccgta gttgtgtacg acggtcttgc cctgaatctg ctcgacgtcg agccgcggtc
     3961 cctgggcacg gaacgggcgc gtgcagaccg tgatcgcagt gacgcggtcg ggactggccc
     4021 gcagcggcgg caactcgagc accgggccgg ctgccgatcg cgacgagccg cgcagcccga
     4081 agctgcagcc cccgagcggg acgccgagga gtgtggcgcc cgttgcgacc aggaattggc
     4141 gccgttcgat agagacaggc ataagctgag ggcggtgcgc cgctcaggag cggagcttca
     4201 gaccctgggg atcgtaaagc ggctcgagcc gcacggtcgc cgcgcgcatc tcgcccagga
     4261 tgtcgatctc gaagcgcgca ccctcgcgcg cgagcgccgt cggcacgtag cccgccgcca
     4321 ggctcttgcc gacgcagtgg ccgaacgcgc ccgaggtcac gtagccgacc gcagcgccgt
     4381 ccctgatgat cgactcgtag ccgatcacct cggcatcgag cgcggccacc tccatgacga
     4441 cgaagcgccg cttcggtccg gccgcgcgct cggcgagcgt cgccgtgcgg cccacgaagt
     4501 ccggcttggt gaaatcgacg aaacggtcga gcccgctctc ggcggcggtg tagtcgggcc
     4561 ggaagtcctt ctgaaaggag ccgtagcctt tctcgaggcg gagcgaagag agcgcgcgcc
     4621 ccccgaacag cctcaggtca agagcgcgtc cggcgtccat gagatcgtcg tag
//
LOCUS       DATLKL010000814         2867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_201583, whole genome shotgun sequence.
ACCESSION   DATLKL010000814 DATLKL010000000
VERSION     DATLKL010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201583"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VM713_11065"
     CDS             complement(<1..394)
                     /locus_tag="VM713_11065"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HVH84843.1"
                     /translation="MNAPLKGGPAAAPGTGQAARPPLRFVSAASLFDGHDAAINMIRR
                     LLQARGAEVVHLGHNRSVADIVRAAIDEDADAIAVSSYQGGHNEYFAYMVDMLRERGY
                     PHIRVVVGGGGTIAPHEIEALERHGVERI"
     gene            633..1250
                     /locus_tag="VM713_11070"
     CDS             633..1250
                     /locus_tag="VM713_11070"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVH84844.1"
                     /translation="MKREAVIRAAARAFNRKGYHNTSLDDIAAALEVTKPTVYYYVTN
                     KEHLLFQCFLAGVEPIRAAFREVKDLELPGRERLMAVLRHYGEAVASEFGWCMVRAEE
                     QDLSPAMSRHIKALKSEIDQGIRRLIREGLQDGSIEPCDPKMSAFALAGALNWIAHWY
                     REDQSLTPAEIAAAFVTLFEGGLRPRTAPQVATRAPLRRRVRIAR"
     gene            1386..2453
                     /locus_tag="VM713_11075"
     CDS             1386..2453
                     /locus_tag="VM713_11075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HVH84845.1"
                     /translation="MQASGVAPADIQEVIMGCVLPANLGQAPARQAALGAGIPQAVPA
                     TTINKMCGSGLKAVMMAAEQIRSGTAEVILAGGLESMSNAPYLLAKARTGYRMGPGEV
                     IDHMMRDGLQSPFDGKAMGFFADATAARYGFTRAQQDAFATESVRRAMAALQGGEFDT
                     EVTPVTVKSRKGDTVIPRDETPGTCDVSKIATMKPAFNKDGTVTAASSSSISDGAAAL
                     VLMKGEVAAARGVKPLARVLAWASHAQEPEWFTTAPVGAIAKVLKQLGWKPHDADLYE
                     INEAFAVVTMAAMRDIDIDHARVNVNGGACALGHPIGATGGRILTTLVHALRRRGGKR
                     GIAALCIGGGEAVALAVEVIK"
     gene            2450..>2867
                     /locus_tag="VM713_11080"
     CDS             2450..>2867
                     /locus_tag="VM713_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVH84846.1"
                     /translation="MKIADARAVVTGGASGLGHAVARHVTAAGGKVVILDVQEGPGRA
                     AAAALGASAGFVRCDVTSEAEVNAAMDAASTRMGGLNLLVNCAGVIGAGRVLGKNGPM
                     AGEFFTKVIHINLIGTFLCDKAAAAIMQNNPPAADGE"
BASE COUNT          447 a         1028 c          990 g          402 t
ORIGIN      
        1 agatccgctc cactccgtgc cgctcgagcg cctcgatctc gtgcggcgcg atggtgccgc
       61 caccgcccac cactacgcgg atatgcggat agccgcgctc gcgcagcatg tcgaccatat
      121 aggcgaagta ttcgttgtgg ccgccctgat aggagctcac cgcgatggcg tccgcgtcct
      181 catcaatggc cgcccggacg atgtcggcga cgctgcggtt atgcccgagg tgcaccacct
      241 cggcgccgcg cgcctgcagc agccgccgga tcatgttgat ggcggcgtcg tggccgtcga
      301 agagcgaggc ggcggacacg aaccgcagcg gcgggcgagc ggcctgcccg gtgccgggag
      361 cggccgcggg gccgcccttg agaggcgcgt tcacgtttta tttactctcg ggtagaatat
      421 ctactcatga gtatgataga cgctccggtc gcgggtcaac gcggcccctg gtacccgcac
      481 actgcggtcc cgcccgcggt caagcccgcc ccggcagcgc cggtataata ctccgcacac
      541 ccatgaacag ccgccgcgcc ggcccgcccc aggcgggcaa atctgccgcc gcgagcccct
      601 ccccgtggcg ggcgcggcgg cgagaccggg aagtgaagcg cgaggccgtg atccgcgccg
      661 ccgcccgcgc gttcaaccgc aagggctacc acaacacctc gctcgacgac atcgctgcgg
      721 ccctcgaggt caccaagccc accgtctact actacgtcac caacaaggag cacctgctgt
      781 ttcagtgctt cctcgccggg gtcgagccga tccgcgccgc ctttcgcgag gtcaaggacc
      841 tcgagctgcc ggggcgcgag cggctgatgg cggtgctgcg ccactacggc gaggcggtgg
      901 cctccgagtt cggctggtgc atggtgcgcg ccgaagagca ggacctctcc cccgccatga
      961 gccgccacat caaggcgctc aagtccgaga tcgatcaggg catccgccgc ctgatccgcg
     1021 aggggctgca ggacggctcg atcgagccct gcgatccgaa gatgagcgcc tttgccctcg
     1081 ccggggctct caactggatc gcccactggt atcgtgagga ccagtcgctg acccccgccg
     1141 agatcgccgc ggcgttcgtc accctattcg agggcggact gcgtccgcgc accgcccccc
     1201 aggtcgcaac ccgcgcgccg ctgcggcggc gggtgcgcat cgcccgctga accacacgag
     1261 gcttccacca tgtccgctcc agacgtagtc atcgcatccg ccaagcgcac gcccatcggg
     1321 gccttcctgg gcgcgctctc ccccctcagc gccgtgcagc tcggcagcgc agccgtcagg
     1381 ccgccgtgca ggcctcgggt gtcgctcccg ccgacattca ggaggtcatc atggggtgcg
     1441 tgctgccggc caacctcgga caggcgccgg cgcgccaggc ggcgctcgga gccgggatcc
     1501 cgcaggcggt gcccgccacc accatcaaca aaatgtgcgg ctcgggcttg aaggcggtga
     1561 tgatggcggc ggagcagatc cgctccggca ccgccgaggt catcctcgcc ggcggactcg
     1621 agtcgatgag caatgcgccg tacctgctgg cgaaggcgcg caccggctac cgcatggggc
     1681 ccggtgaggt gatcgaccac atgatgcgcg acgggctgca gagccccttc gacggcaagg
     1741 cgatgggctt tttcgccgat gcgacggcag cgcgctacgg cttcacgcgc gcccagcagg
     1801 acgccttcgc caccgagtcg gtacggcgcg ccatggcggc gctgcagggc ggggagttcg
     1861 acaccgaggt gacgcccgtg accgtcaaga gccgcaaggg cgacaccgtg attccccgtg
     1921 acgagacccc gggcacctgc gatgtgagca agatcgccac catgaagccc gcgttcaaca
     1981 aggacggcac cgtgacggcc gccagctcct cctcgatctc ggatggcgcc gcggcgctcg
     2041 tgctgatgaa gggcgaggtc gccgcggcgc gcggcgtgaa gccgctcgcg cgggtgctcg
     2101 cctgggcgag ccacgcgcag gagcccgagt ggttcaccac cgcaccggtc ggcgcgatcg
     2161 ccaaggtgct caaacagctc ggctggaagc cgcacgatgc ggatctctac gagatcaacg
     2221 aggcgttcgc ggtggtgacc atggccgcga tgcgcgacat cgacatcgat cacgctcgcg
     2281 tcaacgtcaa cggcggcgcc tgcgcgctcg gccacccgat cggagcgacc ggtgggcgca
     2341 tcctcacgac gctcgtgcac gcgctgcgcc gccgcggcgg caagcgcggc atcgccgcgc
     2401 tgtgcatcgg cggcggcgag gcggtggcgc ttgccgtcga agtgatcaag tgaagatcgc
     2461 cgacgcgcgc gccgtcgtca ccggcggcgc ttcgggtctc ggccacgcgg tggcccgcca
     2521 cgtcaccgcc gcgggcggca aggtcgtgat tctcgacgtg caggagggtc cgggccgggc
     2581 ggcggcggcg gccctcgggg cgagcgccgg cttcgtgcgc tgcgacgtga cctccgaggc
     2641 cgaggtgaat gcggccatgg acgccgccag cacccgcatg ggcggactca atctcctcgt
     2701 gaactgcgcc ggggtgatcg gcgccggacg ggtgctgggg aagaacggac cgatggcggg
     2761 cgagttcttc accaaggtga ttcacatcaa cctgatcggt accttcctgt gcgacaaggc
     2821 cgcggccgcc atcatgcaga acaacccgcc ggcggccgac ggcgagc
//
LOCUS       DATLKL010000815         1797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_134105, whole genome shotgun sequence.
ACCESSION   DATLKL010000815 DATLKL010000000
VERSION     DATLKL010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134105"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1797)
                     /locus_tag="VM713_11085"
     CDS             complement(<1..>1797)
                     /locus_tag="VM713_11085"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HVH84847.1"
                     /translation="NYRAAGDVAVLEIANPPVNALSLAVREGLMQGLARAAADPAIVA
                     VVICGAEGIFPAGADINEIASGLALKAPMILDVQAAMEASAKPLVAAIEGNALGGGFE
                     LALTCHWRVATPAATVGLPEVKLGLIPGAGGTQRFTRLAGPEAALEAITSGAPLTAAR
                     ALELGLIDAVADDAVGAAAELGRQAARERRPLRLASETDERIRAVSGALFTAFRQKIA
                     GRARGQLAPWKIVDCIEAACTRPKAEALRFERDCFVECRDSPQRRALTHLFFAEREAR
                     KIPGVGPDVKPLPIRRAAVIGAGTMGGGIAMNFANAGIPVRVLELSADALERGLAAIA
                     RNYGASVSRGSLTQDRADRARSLIQGVSDYGELGEADIVIEAVFEDLDVKKEVFARLD
                     RVAARHAILATNTSTLDIDALAAVTSRPDKVVGTHFFSPANVMRLLENVRGARSSPET
                     LATVMALARTLGKIAVLAGNCDGFIGNRMLMYYGSEAEFLLEEGATPEQIDRVMEGFG
                     FAMGPLAVRDLAGNDVGFLIRKGRKLPADERFSPILERLVAAGRLGQKAGKGFYRYEG
                     RTRISDPEVVALIEGVSRDLKIRRRQIPDEEIL"
BASE COUNT          260 a          652 c          618 g          267 t
ORIGIN      
        1 aggatctcct cgtcgggaat ctgccggcgc cggattttca ggtcgcgcga gacgccctcg
       61 atgagcgcca ctacctcggg gtcggagatg cgcgtgcgcc cttcgtagcg gtagaaaccc
      121 ttaccggcct tctgcccgag ccgtcctgcg gcgaccagcc gctcgaggat cggcgagaag
      181 cgctcgtcgg cggggagctt gcggcccttg cggatcagga agccgacgtc gttgccggcg
      241 agatcgcgca ccgcgagcgg acccatggcg aagccgaagc cctccatcac ccgatcaatc
      301 tgctctgggg ttgcgccctc ctccagaagg aactccgcct ccgacccgta atacatcagc
      361 atgcggttgc cgatgaagcc gtcgcagttc ccggccagca ccgctatctt gccaagcgtc
      421 cgagcgagtg ccatgacggt cgcgagcgtc tcaggagagc tgcgggcgcc gcgtacgttc
      481 tcgagcagtc gcatcacgtt ggcggggctg aagaagtgcg tgccgaccac cttgtccggg
      541 cgcgaggtca cggcggcgag cgcgtcgatg tcgagcgtcg aggtgttggt cgcgaggatc
      601 gcgtgccgcg ccgccacccg gtcgagccgc gcgaacacct ccttcttcac gtccagatcc
      661 tcgaacaccg cctcgatgac gatgtcggct tcgccgagct cgccgtagtc gctgacaccc
      721 tggatcaggg accgcgcccg gtcggcccgg tcctgcgtca ggctgccgcg cgacaccgag
      781 gcgccgtagt tcctggcgat cgcggccagg ccgcgttcga gcgcgtccgc cgagagctcc
      841 agcacccgca ccgggatgcc ggcgttggca aaattcatgg cgatgccacc gcccatggtg
      901 ccggcaccga tcacggccgc gcgcctgatc ggcaacggct tcacgtccgg ccccacgccc
      961 ggaatcttcc gggcctcgcg ctcggcgaag aacaggtgcg tgagcgcccg gcgctgcggg
     1021 ctgtcgcggc attcgacgaa gcaatcgcgc tcaaagcgca gcgcctccgc cttcgggcgg
     1081 gtgcaggccg cctcgatgca gtcgacgatc ttccacgggg ccagctggcc gcgcgcccgc
     1141 ccggcgattt tctggcggaa tgccgtgaac agcgcgccgc tcacggcgcg gattcgctcg
     1201 tcggtctcgc tcgcgaggcg cagcggccgc cgctcgcgtg ccgcctgcct gccgagctcg
     1261 gccgcggccc ccaccgcatc gtccgccacg gcgtcgatga gccccagctc gagggcgcgc
     1321 gccgccgtga gcggggcgcc ggaggtaatc gcctcgaggg cggcctcggg cccggcgagc
     1381 cgcgtgaagc gctgcgtgcc gcccgctccg ggtatgagcc cgagcttcac ttctggcaga
     1441 cccaccgtgg cggcgggcgt cgccacgcgc caatggcagg tcagcgccag ctcgaaccca
     1501 ccgccgagcg cattgccctc gatggccgcg accagcggct tggcgctcgc ctccatggcc
     1561 gcctgcacgt cgagaatcat cggagctttg agcgcgagcc cggaagcgat ctcgttgatg
     1621 tcggcgccgg cgggaaagat gccttcggcg ccgcatatca ccaccgcgac gatcgccgga
     1681 tccgcagcgg cgcgggccag accctgcatg agtccctcgc gcaccgcgag gctgagggcg
     1741 ttaacgggcg ggttggcgat ctccaggacg gcgacgtcgc cggcggcgcg atagttg
//
LOCUS       DATLKL010000816         3589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_122784, whole genome shotgun sequence.
ACCESSION   DATLKL010000816 DATLKL010000000
VERSION     DATLKL010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122784"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..771)
                     /locus_tag="VM713_11090"
     CDS             complement(7..771)
                     /locus_tag="VM713_11090"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HVH84848.1"
                     /translation="MKNAAMWARTCGAVVLTLLLAAASAQAQDRDRGDHRGERSERGD
                     RGDRGDRGDRGSFRPGGRAPEHMDGRYAHNRYYPDRGEYVRGVPGRPYVFDRPGGRYY
                     YSGGVWYRPYGESFVVIGAPVGLFVPVLPPYYSTMWVGGVPYYYANDTYYVWSQAQNG
                     YEVVDPPDDQGASPDAPPPPPPQGDNLFIYPQQGQSAEQQAPDQYECHKWSSAQTGFD
                     PTQSGGGVPPDQLGSRRADYQRAMIACLQGRGYSAR"
     gene            complement(792..1382)
                     /locus_tag="VM713_11095"
     CDS             complement(792..1382)
                     /locus_tag="VM713_11095"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HVH84849.1"
                     /translation="MERFPMMKPTRKCRRTSLAALAVGAAAAVLAGCVEAPPPRPVYD
                     PPPPPPPPPVSQVYAYPLHGQTAEQQDRDHYECSTWATQQTGFDPSAPGVAPHDRVQV
                     VSAGPPPGTNTAIGAVAGAIIGAAIAPRWQAAPAALAGAVVGGAIGSTADAANAESTR
                     TVVVADRRAAAAEEQKALAYRRAISACLDARGYSVK"
     gene            complement(1561..2319)
                     /gene="kdsB"
                     /locus_tag="VM713_11100"
     CDS             complement(1561..2319)
                     /gene="kdsB"
                     /locus_tag="VM713_11100"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HVH84850.1"
                     /translation="MFRVVIPARYDSVRLPGKALQPIGGKPMVQWVYERARAAGALEV
                     LIATDDARILEAARACGAAAVMTAATHLSGTDRIAEVAGARHWPERDIVVNLQGDEPL
                     MPPALIAQVAALLDSTPAADVATLATGIGSVEELLDPNAVKVVTDARGRALYFSRAPI
                     PWNRAGAGLASQTDPGGARRHIGIYAYRAGPLRRLAALAPGALELREKLEQLRALEHG
                     LHIQVADACEPPGPDVNTPADLARVAAALGGAPA"
     gene            complement(2333..2521)
                     /locus_tag="VM713_11105"
     CDS             complement(2333..2521)
                     /locus_tag="VM713_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HVH84851.1"
                     /translation="MDYRLLEILACPVCKGTLKYQREAQLLVCRMDRLAYPIRDDVPV
                     MLEEEARQLAADDPLLER"
     gene            complement(2529..3548)
                     /gene="lpxK"
                     /locus_tag="VM713_11110"
     CDS             complement(2529..3548)
                     /gene="lpxK"
                     /locus_tag="VM713_11110"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HVH84852.1"
                     /translation="MRERLERLWYEDRTGAPLLAPLAFVYGALEAARRSAYRSGLLRS
                     RRLARPVVVVGNLTVGGTGKTPLTIHLARELSAAKLKVGVVSRGYGRRGGDARYVHPD
                     SDWREVGDEPVIIARRTGCATMVAADRVKAARTLIARGAHVILADDGLQHLSLGRDCT
                     IVVIDGSRGFGNGRLLPAGPLREPVAALGRADLVVVNGEPEHGSLATALPAGTLTMRL
                     VAESAVRLDGRGLPCELSAFSGHRVHAVAGIGNPQRFFRELSRHGIEVIAHPFPDHHP
                     FTAHELAFADELPVLMTEKDAVKCAPFANARLWCVPVSAEFSASDAQALRERVLGRLR
                     ARYGG"
BASE COUNT          536 a         1269 c         1234 g          550 t
ORIGIN      
        1 cggaccctag cgggcgctgt agccgcgccc ctggaggcag gcaatcatgg cgcgctgata
       61 gtcggcgcgc ctcgacccga gctgatcggg aggcacgccc ccgccggact gcgttggatc
      121 aaagccggtt tgcgccgacg accacttgtg gcactcgtac tgatctggcg cctgctgctc
      181 cgcgctctga ccctgctggg ggtagatgaa gagattgtcg ccctgcggcg gcgggggtgg
      241 cggcgcatcg ggcgaggctc cctggtcgtc cggcgggtcc acgacctcat agccgttctg
      301 cgcctggctc cacacatagt aggtgtcgtt ggcgtagtaa tacggaacgc cgccgaccca
      361 catcgtcgag tagtacggcg gcagcaccgg aacgaacagc ccgaccgggg caccgatcac
      421 gacgaagctc tcgccatagg gccggtacca cacgccgcct gaatagtagt agcggccgcc
      481 tggccgatcg aacacgtacg ggcgtcccgg caccccgcgg acgtactcac cgcggtcggg
      541 atagtagcga ttgtgggcgt agcgcccatc catgtgctcg ggcgcgcggc cgcccgggcg
      601 gaagctaccg cgatcgccgc ggtcaccgcg gtcaccccgg tcaccgcgtt cactgcgttc
      661 accgcgatga tccccgcgat cgcggtcctg agcctgcgca ctcgctgccg cgagcagcag
      721 ggtgaggacc acagccccgc atgtccgcgc ccacattgcc gcattcttca tgatcgctct
      781 ctatcttgac ttcacttcac actgtagccg cgcgcgtcga gacacgcgga gatcgcgcgt
      841 ctataggcca gcgccttctg ctcctcggcg gccgccgcac gccgatcggc gaccaccacg
      901 gtgcgggttg actccgcgtt ggccgcatcg gccgtgctgc cgatcgcgcc tcccaccacc
      961 gcgcccgcca gcgccgcggg tgcggcctgc cagcgcggcg caatcgccgc gccgatgatc
     1021 gcgccggcga cggcgccgat ggcggtattc gttcccggag gtggacctgc ggagactacc
     1081 tgcacgcgat cgtgaggcgc gacgccgggt gcgctcggat cgaagccggt ctgctgcgtc
     1141 gcccaggtgc tgcactcata gtggtcgcgg tcctgctgct ccgccgtctg gccgtgcagc
     1201 gggtaggcgt acacctgcga gaccggcggt ggcgggggcg gcgggggcgg gtcgtacacg
     1261 ggtcgcggcg gcggtgcttc tacgcagcca gcgaggaccg ccgcggcggc ccccacggcc
     1321 agcgcggcga gagatgtgcg gcggcatttg cgggtgggtt tcatcatcgg gaatctttcc
     1381 atgtcgcgag cgaatcgctc tttatcacaa cgttaggtgc gggcctgggg ttgacactcg
     1441 aggccccctg ccccgactca aattttgacg gcgcgccaag ggcttaagtt cctctcatgt
     1501 gagcaattgt tcaccgttcc gccggccgct tcagtcagca ctgaccgcta ttttaccggc
     1561 tcaggcgggt gcgccgccaa gcgccgccgc aacccgcgcg agatctgcgg gagtgttgac
     1621 gtccggcccc ggcggctcgc acgcgtcagc cacctgaatg tggaggccat gctccaatgc
     1681 gcgcagctgc tctagcttct cgcgcagctc gagcgccccg ggtgcgagcg ccgcgagccg
     1741 cctgagcggg ccagcccggt aggcgtagat ccctatgtgc cgccgcgcgc cgcccggatc
     1801 agtctgactc gcgaggcccg cgccggcgcg attccaggga atcggcgccc gactgaagta
     1861 cagcgcgcgc ccacgcgcgt cggtcaccac ctttaccgca ttggggtcga ggagctcctc
     1921 gaccgagccg atgccggtcg cgagcgtcgc gacgtcggct gcgggggttg aatccaacaa
     1981 cgccgccacc tgggcaatga gcgccggggg catcaggggc tcgtcgccct gcagattcac
     2041 gacgatgtcg cgctccggcc aatgccgtgc gccggctacc tcggcgatgc ggtcggtgcc
     2101 cgagaggtgc gtcgccgccg tcatcaccgc cgcggcgccg caggcgcgcg cggcctcgag
     2161 gatgcgcgca tcgtcggtgg ctatcagtac ttcgagtgcg ccggcggcgc gcgcgcgctc
     2221 gtatacccac tggaccatgg gcttgccgcc gatcggctgc aacgccttgc cggggagcct
     2281 gaccgagtca tagcgcgcgg gaatgactac ccggaacacg ggcggcgtgc tttcagcgct
     2341 cgagcagcgg gtcgtcggcg gccagctggc gcgcctcctc ttccagcatc accggcacgt
     2401 cgtcgcggat cggataggcg agccggtcca tgcggcacac cagcagctgc gcctcgcgct
     2461 ggtacttgag cgtccccttg catacggggc aggcgagtat ctcgagcagt cggtagtcca
     2521 tcggcgctct aacctccgta ccttgcgcgc aacctgccaa gcacgcgctc gcgcagcgcc
     2581 tgcgcatcgc tcgcgctgaa ctcggcgctg accggcacgc accataaccg cgcgttggcg
     2641 aacggcgcgc attttacggc atctttctcg gtcatgagga ccggcagttc gtcggcaaag
     2701 gcaagctcgt gcgcggtgaa cgggtgatga tccgggaagg gatgcgcgat cacctcaatg
     2761 ccgtgacggg agagctcgcg aaagaaacgc tgcgggttgc cgatcccggc caccgcgtgc
     2821 acccggtggc cgctgaaggc gctgagctcg cagggcaatc cgcgcccgtc gagccgcacg
     2881 gccgactctg ccacgaggcg catcgtgagt gtgccagcgg gcaacgccgt cgcaagtgag
     2941 ccgtgctccg gctcgccgtt cacgactacg aggtcggccc ggccgagcgc cgccaccggc
     3001 tcgcgcaggg gtcccgccgg cagcaggcgg ccgttaccga atccgcggct gccgtcgatc
     3061 acgacgatcg tacagtcacg gccgagggag agatgctgca gcccgtcgtc ggcgagaatc
     3121 acgtgagcgc cgcgggcgat gagcgtgcgg gccgccttga cccggtcggc ggccaccatg
     3181 gtcgcgcatc cggtacggcg cgcgatgatc accggctcat cgccgacctc gcgccagtcc
     3241 gagtccggat gcacgtagcg tgcgtccccg ccgcgccgcc cgtagccgcg cgacacgaca
     3301 cccaccttga gcttcgcagc actgagctcg cgtgccaggt ggatggtgag cggcgtcttg
     3361 ccggtgccgc cgaccgtaag gttgccgacc accacgacgg gacgggccag acgacgggac
     3421 cgcagcaggc ccgagcggta ggcgctgcgc cgtgcggcct cgagggcgcc atagacgaag
     3481 gcgagcggcg ccaggagcgg agctcctgtg cggtcctcgt accacagccg ctcgagccgc
     3541 tcccgcacgc gtgcgccgcc tcagtccgaa aactgcagct gatgcaact
//
LOCUS       DATLKL010000817         2254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_66389, whole genome shotgun sequence.
ACCESSION   DATLKL010000817 DATLKL010000000
VERSION     DATLKL010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66389"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VM713_11115"
     CDS             <1..717
                     /locus_tag="VM713_11115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVH84853.1"
                     /translation="KWAKAAFPQVREFFFDDDTLTDNLPRVEALAVELGKLGVTWSCN
                     AKANVPRKTLEVMKANGLRLLLVGYESGNQQILFNIKKGMRVEFARRFTRDCHALGIR
                     VHGTFILGLPGETRATIQETLDFAKEINPHTIQVSLAAPYPGTFLYRQAMQNGWLRDA
                     EADLLTAQGTQVATLSYPHLSHTEIFASVDEFYRKFYFRPAKIAALLAEMLTSRELMR
                     RRLREGMEFLQFLRSRRDAA"
     gene            714..1607
                     /gene="hpnK"
                     /locus_tag="VM713_11120"
     CDS             714..1607
                     /gene="hpnK"
                     /locus_tag="VM713_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HVH84854.1"
                     /translation="MKRLIVTADDFGAAREVNEAVEQAHLRGILSAASLMVAAPAAAD
                     AIARARRLPTLRVGLHVVLTDGRPLLPAAAVSRLIDASGAFRGNLALLGAAIAASSAV
                     RRQVAAEISAQFARFCDSGLELDHVNAHKHFHLHPVVAALLITIGRRFGLRAVRLPLE
                     PPRVLRRVARSARWQPQLMLPALLLRRRLKSAGLLAPDQCFGIEWSGRMTRPRLLALL
                     ASLPPGLTEIYLHPATAADFPGAAPGYRYREEFEALVAPDVVAACRGSSLTLGGFRDF
                     LTCGVTTLPVPAHRGDLSLRM"
     gene            1612..>2254
                     /locus_tag="VM713_11125"
     CDS             1612..>2254
                     /locus_tag="VM713_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84855.1"
                     /translation="MSSASPISPSTADGLSRIRLGSDAHKELFCRTLLDTFNPYEPAK
                     IAWPELERPAHERLVNLPIWDLAVQTEGRARLNVASYAATVRDPLLHEALELNAFEEG
                     RHRDVLSGMVAAYGIPLAPEPSYVLRGDPEWAFMRTGYSECIDSFLSFGLFVSARRSG
                     FFPPALVAAFEPVVQEEARHILFFVNWAAWHRRTMPLWRRPWFALKELAIWLAL"
BASE COUNT          333 a          798 c          733 g          390 t
ORIGIN      
        1 aaatgggcca aggcggcgtt tccgcaggtg cgggagttct tcttcgacga cgacaccctc
       61 accgacaacc tgccgcgcgt cgaagcgctg gcggtcgagc tcggcaagct cggggtcacc
      121 tggtcctgca acgccaaggc caacgtaccg cgcaagacgc ttgaggtgat gaaagccaat
      181 gggctgcggc tgctgctggt cggctacgag tccggcaatc agcagatcct tttcaacatc
      241 aagaagggca tgcgggttga gttcgcacgg cgcttcacgc gcgactgcca tgcgctcggc
      301 atccgcgtcc acggcacttt cattctcggc ctccccggag agacgcgcgc gacgatccag
      361 gaaacgctcg atttcgccaa ggagatcaat ccacacacga tccaggtgtc gctggcggct
      421 ccgtatccgg gcacctttct ctaccgccaa gcgatgcaga atggctggct gcgcgacgcg
      481 gaggcggatc tcctcaccgc gcagggaacg caggtcgcga cgctcagcta cccgcatttg
      541 agccacaccg agatattcgc ctcggtggat gagttctatc gcaagttcta cttccgcccc
      601 gcgaagatcg ccgcgctcct cgccgagatg ctcaccagcc gcgagctgat gcggcggcgg
      661 ttgcgcgagg ggatggagtt cctgcagttc ctgcgctcgc gccgggatgc ggcgtgaagc
      721 gcctcatcgt caccgccgac gactttggcg ccgcgcgaga ggtcaacgag gccgtcgagc
      781 aggcacatct gcgcggtatc ctctccgccg cgagcctcat ggtcgcagct ccagcggcgg
      841 ctgacgccat cgcccgggcg cgccggctcc cgacgctgcg ggtcgggttg catgtcgtgc
      901 tgaccgacgg gcggccactg ctgccggccg cggccgtgag ccgactgata gatgcgagcg
      961 gcgccttccg cggcaatctc gcgctcctcg gggccgccat cgccgcaagc tccgcggtgc
     1021 ggcgccaggt cgccgcggag atcagcgccc agttcgcgcg cttttgcgac agcggcctcg
     1081 agctcgatca cgtcaacgcg cataagcact ttcatctgca ccccgtggtc gccgcgctgc
     1141 tgatcacaat cggccgccgc ttcggcctgc gcgccgtgcg gctgccgctc gagccgccgc
     1201 gggtgctgag gcgggtcgcc cgctccgcac gctggcagcc gcagctgatg ctcccggcgc
     1261 tactgctgcg gcggcggttg aagtccgcgg gtctcctcgc tcctgaccag tgcttcggca
     1321 tcgagtggtc gggacgaatg acacgtccgc gcctgctcgc gctgctcgcg agcctgccgc
     1381 ccgggctcac cgagatctac ctgcacccgg cgaccgcggc cgattttccc ggagcggcac
     1441 ccggctatcg ctaccgcgag gaattcgagg cgctggttgc tcccgacgtc gtcgcggcct
     1501 gccgcggctc atcgctgact ctcggcgggt tcagagactt ccttacttgc ggggtgacga
     1561 ccctgccggt accggctcac cgcggcgacc tgtcgctccg catgtgagcc catgagctcg
     1621 gcctccccga tatcgcccag cactgcggac ggcctatccc ggatccgtct ggggagcgat
     1681 gcgcacaagg agctcttctg tcgcactctg ctcgacacgt tcaatcccta cgagcccgcg
     1741 aagatcgcct ggcccgagct cgagcgccca gcgcacgagc ggctcgtgaa tctcccgatc
     1801 tgggacctcg cggtgcagac cgagggacgc gcgcgcctca acgtcgcaag ctacgcggcg
     1861 acggtgcgcg acccgctgct gcacgaggcg ctcgagctca atgccttcga ggaggggcgc
     1921 caccgggacg tgctgtcagg aatggtcgcg gcgtacggca tcccgctcgc ccccgagccg
     1981 agctacgtgc tgcgcggcga tcctgagtgg gccttcatgc gcaccgggta cagcgagtgt
     2041 atcgacagct ttctttcctt cggactcttc gtgtcggccc ggcgctccgg attctttcca
     2101 ccggcgctgg tcgcggcctt cgagccagtc gtgcaggaag aggctcggca catcctattc
     2161 ttcgtcaact gggccgcctg gcatcgccgc accatgccgc tgtggcgccg gccatggttc
     2221 gcgctgaagg agctggccat atggctggcg ctcg
//
LOCUS       DATLKL010000818         2088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_122883, whole genome shotgun sequence.
ACCESSION   DATLKL010000818 DATLKL010000000
VERSION     DATLKL010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122883"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..858)
                     /locus_tag="VM713_11130"
     CDS             complement(70..858)
                     /locus_tag="VM713_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84856.1"
                     /translation="MMRTAWSRLIAAAGAALAIGLGIAACNGSGYSRATTPTVPTATG
                     IWEGTDSVTKLSVTGYVDSANNAVFIRGDGAQFVGPTQLSGDTFIAAVIGYSSFPAAF
                     ADGSNYGVGTLNGTVAPGSTLTLTLSFTTNGGTAEAGSWSLTYSTLSSSGSSLTKISA
                     NYTDTASGSVISISGNGVMTGQDPANSCVVNGTVSIVNSTYDIYQVTLTYENCTGSYA
                     VLNGVQLTGLAVLNTSTTPSQLTIELAGASTTAKFALVLNLTGA"
     gene            980..1999
                     /locus_tag="VM713_11135"
     CDS             980..1999
                     /locus_tag="VM713_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVH84857.1"
                     /translation="MTYPFAAAPEPGQALEVAPGVRWLRMRLPMAALNHINVWALEDG
                     AGWTLVDTGMDLPDTVAGWQSALSGPLGARPVVRVICTHMHPDHIGMAGWLTRQHECR
                     LWTTRLEYVTCRMLVADTGREAPADGVRFYRAAGWDDAALEHYRARFGGFGKAVYALP
                     DSYRRVVDGEELPIGGRSWRGVVGRGHSPEHLCLYCPALRVLISGDQVLPRITSNVSV
                     FPTEPDADPLSEWLTSLASIRERVSDDVLVLPSHNEPFRGLHARLDALIEGHEERLAR
                     LAAELAAPRRAVDVFALLFRRRVGVEMLSMATGESVAHLNCLMARGLVVRERDAAGVA
                     WYRRT"
BASE COUNT          313 a          679 c          721 g          375 t
ORIGIN      
        1 catccaggtc gccgcgcgct cgggtcccgc tgcctgcatc gcgggcctca ggacgccggt
       61 ggggccagct caggcgccgg tgagattaag gacgagcgca aacttggcgg tggtggaagc
      121 gccggccagc tcgatcgtca actggcttgg cgtggtgctg gtattcaaga cggcgaggcc
      181 ggtgagctgg acgccgttca ggacggcgta gctcccggtg caattctcgt acgtcaaggt
      241 cacctggtag atatcgtagg tgctgttgac gatgctgacc gtgccattca ccacgcagct
      301 gttggcgggg tcctgtccgg tcatgacgcc attgccagag atcgagatca ccgagccgct
      361 ggccgtatcg gtgtaattcg cgctgatctt ggtcagagac gagccgctgc tgctcagagt
      421 gctgtaggtg agcgaccaag agcccgcctc cgcggtcccg ccgttggtcg tgaaactgag
      481 agtcaacgtg agcgtgctgc cgggcgccac ggtgccgttc agtgtgccga ccccgtagtt
      541 cgagccgtcc gcgaaggccg cggggaaact cgaatagccg atcacggcag cgatgaaggt
      601 gtcccccgag agctgcgtcg ggccgacgaa ctgagcgccg tcaccacgga tgaagaccgc
      661 gttgttcgcc gaatcgacat agccggtcac gctcagcttc gtgaccgagt cggtgccttc
      721 ccagatgccg gtggccgttg gcaccgttgg ggtcgtcgcg cggctataac cgctgccgtt
      781 gcaagctgcg atcccgagcc cgatcgcgag cgcggcgccg gccgctgcga tgagcctgct
      841 ccaggctgtg cgcatcatta tcgttccgcc ctgtccttcg acgccctcta acgcgccaga
      901 cggcacgcgg ctgacagctg cctgttccgc gcccacgcga tagcctacat tgttcgctat
      961 gcaggacgcc cctggggtat tgacctaccc gttcgctgcg gctccggagc ctgggcaggc
     1021 gctcgaagtc gcaccgggtg tgcgctggct gcgcatgcgc ctgcccatgg cggcgctcaa
     1081 tcacattaat gtctgggcgc tcgaggacgg ggcgggctgg acgctggtcg atacgggcat
     1141 ggacctgccg gacaccgtag cgggatggca gagcgccctc tccggcccgc tcggagcgcg
     1201 cccagtggtc cgtgtcatct gcacgcacat gcatcccgat cacatcggga tggcgggctg
     1261 gctcacccga cagcacgagt gccggttgtg gacgacgcgc ctcgagtatg tgacctgccg
     1321 catgctggtg gcggacacgg ggcgcgaggc gccagcagac ggcgtgcggt tctatcgcgc
     1381 cgccggctgg gacgatgccg cgctcgagca ctaccgcgcg cgcttcggcg gcttcggcaa
     1441 ggcggtctac gcactgccgg acagctaccg gcgcgtggta gacggcgaag agttgccgat
     1501 cggcggacgc agctggcgcg gtgtcgtcgg ccgcggccac tcgcctgaac atctgtgtct
     1561 ttattgcccg gcgctgcgcg tgctgatctc gggcgatcag gtgctgcccc gaattacctc
     1621 gaacgtctcg gtcttcccca ccgagccgga cgcggatcca ctctcggaat ggctcacctc
     1681 gctcgcctcg atcagggagc gggtgagcga tgatgtgctc gtcctgccct cgcacaacga
     1741 gccgttccgc ggcctgcatg cgcggcttga cgcgttgatc gaggggcacg aggagcggct
     1801 cgcgcgcctc gcggccgagc tcgccgcccc gagacgcgcg gtagacgtgt tcgctctgct
     1861 gtttcgccgc cgcgtcggcg tcgagatgct cagcatggcg accggtgaga gtgtggcgca
     1921 cctcaactgc ctcatggctc gcggtctcgt cgtgcgcgag cgcgatgcgg ccggggtggc
     1981 ttggtatcgg cgcacttgag ccgccgccac gctagtcaca atggaacgac cggctcccga
     2041 tttgcaaagc gcggattacg ctttggatcg ggtaagaccc gcggcgta
//
LOCUS       DATLKL010000819         3607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_190631, whole genome shotgun sequence.
ACCESSION   DATLKL010000819 DATLKL010000000
VERSION     DATLKL010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190631"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VM713_11140"
     CDS             complement(<1..1072)
                     /locus_tag="VM713_11140"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HVH84858.1"
                     /translation="MSTAEPTDPQRPPPSVFLSYASEDRPAAQALRDALQTYGLEVWY
                     DESGLDGGDAWDQKIRRQIRDCDFFMPVISAQTDARPEGYFRREWRLAVERTHDMADD
                     HTFLLPIVIDETVQARARVPEKFLAVQWTRVPGGQPTAALEAVCQRLLSGHGAAPDPA
                     GEAPGRPAQAHSRRAPRAYPEFPREEPGQRVRFWAHVVGWLLQAAWISFGRLPRWIRW
                     IVWAWLAIALMSRGCSRHWDNDESPPATPKVGSGKAISPADANKLREIADSYQGSANS
                     SDVAKLGLQIAQTFASEVGKEVAAAQAPLLAIPFGAPAGDAPGQKLADSTFAQVYGRI
                     AISHHGHVGLMSEPLASLDTASA"
     gene            1115..2080
                     /locus_tag="VM713_11145"
     CDS             1115..2080
                     /locus_tag="VM713_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVH84859.1"
                     /translation="MPGFHSGTGASGSRAVVTVAGLRKTYANGLTALDDIELTIERGE
                     IFALLGPNGAGKTTLINVICGIVTPSAGRVVADGHDIVSDYRAARSKIGLVPQELHTD
                     TFESVWATVNFSRGLFGRAPDPAYCERILRDLSLWDRKDAKIMTLSGGMKRRVLIAKA
                     LAHEPTILFLDEPSAGVDVELRRDMWQLVRRLRDSGVTIILTTHYIEEAEEMADRIGV
                     IHKGRLIVVEDKSLLMRKLGKRQLILHLQQPLAALPAGLSAYPLELADGGATLVLTFD
                     AHRGEGGIAPFLKRLGELGIDFKDLHSSESSLEDIFVGLVSERRP"
     gene            2077..2838
                     /locus_tag="VM713_11150"
     CDS             2077..2838
                     /locus_tag="VM713_11150"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVH84860.1"
                     /translation="MNVFAIRAIYRFEMARTFRTLMQSIAAPVLSTALYFVVFGAAIG
                     ARMGRVDGVGYGSFIVPGLIMLSILTESVANASFGIYLPKWSGTIFELLSAPVSYVEV
                     LIGYVGAAATKSVMLGVLILVTARVFVPYEVLHPAWMVAFLILTAVSFSLFGFILGGW
                     AGSFEQLQVVPLMILTPLTFLGGAFYSIGMLPPLWQKITLFNPVVYLISGFRWSFYGT
                     ADVNVAASAAATLLFLALCVAAVAWIFRTGYKLKN"
     gene            complement(2961..>3607)
                     /locus_tag="VM713_11155"
     CDS             complement(2961..>3607)
                     /locus_tag="VM713_11155"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVH84861.1"
                     /translation="NNGVYGLTKGQFSASADVGTKAKKGETNFQPPIDPVLLAMTLGA
                     SFVARSFSGDKQQLVPLIQAGLKHRGFALIDVLSPCVTFNDHEGSTKSYAYTREHYEP
                     AVHADFVPLEREISVDYQEGETVPVVMHDGSRIVLRKLDSAYDPTNRASAAAHIQERM
                     KAGEYLTGLLFLDEASQHEFHQVNSTPAVPLNSIPYETLSPGSQGLEKILARYR"
BASE COUNT          557 a         1190 c         1214 g          646 t
ORIGIN      
        1 cagccgaggc ggtgtcgagg gaggcgagcg gctcgctcat caggccgacg tgtccgtgat
       61 gggaaatggc gatccggccg tacacctgag cgaacgtgga gtcggcgagc ttctggcccg
      121 gtgcgtcccc ggcaggtgct ccgaacggga tggccaacag cggggcttgt gccgccgcga
      181 cctccttgcc gacttcgctg gcgaaggttt gtgcgatctg caggcctagc ttggcgacgt
      241 cggagctgtt cgcgctgccc tgatagctgt cggcgatttc gcgcagcttg ttggcatccg
      301 ccggtgagat cgctttgcca cttcctactt tcggcgtagc gggcggcgac tcatcgttgt
      361 cccagtgccg cgagcagccc ctcgacatca gcgcaatcgc gagccaggcc cacacgatcc
      421 atcttatcca gcggggcagc cgcccgaatg atatccaggc cgcctgcagc agccagccga
      481 cgacgtgcgc ccagaaacgg acccgctgtc ccggctcctc gcgcgggaat tcggggtacg
      541 cgcgcggcgc ccggcgcgag tgcgcctggg cgggccggcc gggtgcctcc ccggcggggt
      601 ccggcgcggc cccatggccc gagaggagcc gttgacagac cgcctcgagc gctgccgtgg
      661 gctgcccgcc cgggacgcgg gtccactgca ccgcgagaaa cttctcagga acgcgggcgc
      721 gcgcctgcac ggtctcatca atgacgatcg gcagaagaaa ggtgtgatcg tccgccatgt
      781 cgtgggtccg ctcgacggcc aggcgccact ccctgcggaa gtagccctcg ggacgcgcgt
      841 cggtttgcgc ggatatgacc ggcatgaaaa aatcgcagtc gcgtatctgt ctgcggatct
      901 tctggtccca ggcgtctcct ccgtcgagcc cgctctcgtc gtaccagacc tcgagtccat
      961 acgtctgcag agcgtccctg agggcctgtg ccgccgggcg atcctccgag gcatagctca
     1021 ggaacaccga aggagggggg cgctgcggat cggtgggctc ggcggtggac atgaggtggc
     1081 gggccagtgt tgcttgaaca aggttagggc aaaaatgccc ggatttcact caggtaccgg
     1141 ggcgtccggt tcccgggcgg tcgtgaccgt ggcgggtctg cgcaagacct acgccaacgg
     1201 cctcaccgcg ctcgacgaca tcgagctcac gatcgagcgc ggcgagatct tcgcgctgct
     1261 cggccccaac ggcgccggca agactacgct catcaacgtc atctgcggca tcgtcacgcc
     1321 gagcgccggc cgcgtggtcg ccgacggtca cgacatcgtg agcgactacc gcgcggcgcg
     1381 cagcaagatc ggcctggtgc cgcaggagct gcacactgac acgttcgaaa gcgtctgggc
     1441 gacggtcaat ttcagccgcg ggctgttcgg ccgggcgccg gatccagcct actgcgagcg
     1501 catcctcagg gatctgtcat tgtgggaccg caaggacgcg aagatcatga cgctgtccgg
     1561 aggcatgaag cggcgcgtgc tgatcgccaa ggcgctcgcg cacgagccga cgatcctgtt
     1621 cctcgatgag ccgagcgcgg gcgtcgacgt cgagctgcgg cgcgacatgt ggcagctggt
     1681 gcgccggctg cgcgacagcg gcgtgaccat catcctcacc acgcattaca tagaagaggc
     1741 cgaggagatg gccgaccgga tcggcgtgat tcacaaggga cggctgatcg tggtcgagga
     1801 caagtcgctg ctgatgcgca agctcggcaa acggcagctg atcctccacc tgcagcagcc
     1861 gctcgcggcg cttccggccg ggctctccgc ctatccgctc gagctcgccg atggcggcgc
     1921 gacgctggtt ctcaccttcg acgcccatcg cggcgagggc ggcatcgcgc cgttcctcaa
     1981 gcgcctcggc gagctcggca tcgacttcaa ggatctgcac tcgagcgaga gctcgctcga
     2041 ggacattttc gtgggcctgg tgagcgagcg gcgcccatga acgtcttcgc gatccgcgcg
     2101 atctatcgct tcgagatggc gcggaccttc cgcaccctga tgcagagcat cgcagccccg
     2161 gtgttgtcga ccgcgctgta cttcgtggtc ttcggcgccg cgatcggcgc gcgcatgggc
     2221 agagtcgacg gcgtcggcta cggctccttc atcgtgccgg gcctgatcat gctgtcgatc
     2281 ctcaccgaga gcgtcgccaa cgcctccttc ggcatctacc tccccaagtg gtcgggaacg
     2341 atcttcgagc tgctctcggc gcccgtctcc tatgtcgaag tgctgatcgg ctacgtgggg
     2401 gctgcggcca ccaagtcggt gatgctcggg gtgttgatcc tcgtgaccgc gcgcgtgttc
     2461 gtgccctacg aggttctgca tccggcgtgg atggttgcgt tcctcatcct tacggccgtc
     2521 tccttcagcc tgttcggctt catcctcggc ggctgggccg gcagcttcga gcagctgcag
     2581 gtggtaccgc tcatgatcct caccccgctc accttcctcg gcggcgcctt ctactcgatc
     2641 ggcatgctgc cgccgctgtg gcagaagatc acgctcttca atccggtcgt ctacctgatc
     2701 agcggcttcc gctggagctt ctacggcacg gcagacgtga acgtggcagc gagcgcggcg
     2761 gccacgctgc tgttcctggc gctgtgcgtc gcggcggtgg cttggatctt caggacaggc
     2821 tacaagctga agaactagtc gaccgccggc aaagagcggg gctcgacacg cagcagtccc
     2881 aatcacctcg gcagccgagc actccgtagc aaggtcggcg cacgggtttt tgccggctgc
     2941 gccccgggga gcgattctgc ctaacgataa cgggcgagga tcttctcgag tccctgcgag
     3001 cccggcgaca gcgtctcgta agggatgctg ttgagcggca ccgcgggcgt cgagttcacc
     3061 tggtggaact cgtgctgcga ggcctcatcg aggaacagca gccccgtcag gtactcgccg
     3121 gccttcatgc gctcctggat gtgtgccgcg gcgctcgcgc ggttggtcgg atcgtaggcg
     3181 gagtcgagct tgcgcagcac gatgcggctg ccgtcgtgca tcaccaccgg gacggtctcg
     3241 ccttcctgat agtcgacact gatctcacgc tcgagcggca cgaagtccgc gtgcaccgcc
     3301 ggctcgtaat gctcgcgcgt gtaggcgtag ctcttggtcg agccctcgtg gtcgttgaag
     3361 gtcacgcagg gcgagagcac gtcgatcagc gcgaagcccc ggtgcttgag ccccgcctga
     3421 atgagcggca ccagctgctg cttgtcgccg gagaagctgc gcgccacgaa cgaggcgccc
     3481 aaggtcatgg ccagcaacac cgggtcgatg ggcggttgga agttggtctc gcccttcttc
     3541 gccttggtgc cgacgtcggc cgaggcggag aactgtccct tggtgagccc gtacaccccg
     3601 ttgttct
//
LOCUS       DATLKL010000820         5174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_190643, whole genome shotgun sequence.
ACCESSION   DATLKL010000820 DATLKL010000000
VERSION     DATLKL010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190643"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VM713_11160"
     CDS             complement(<1..347)
                     /locus_tag="VM713_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVH84862.1"
                     /translation="MATQHGGCVALLSGEAGIGKTALLREFAKRQEQARVLWGACDAL
                     FTPRPLAPLHDVALQTQGALQAAVAAHGSPTALFAATLGELGRAKTLLILEDLHWADE
                     ATLDLLTFLGRRI"
     gene            complement(419..1051)
                     /locus_tag="VM713_11165"
     CDS             complement(419..1051)
                     /locus_tag="VM713_11165"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVH84863.1"
                     /translation="MAGGKYLLYGRRGAGSLAPQILLEEIGAPYELIWVGTTAAELET
                     LRRVNPAGKIPSLVLPNGTAVAESAAILIHLTNAHPSAALAPAAGSTAHARFLQWMVF
                     MSASLYDAVLRYYYSERYSTSGAEAAPAIKAQALADYGAHLERIHAELSPYVLGDKFS
                     AVDPYLHMLTGWYPEDAQQLAARMPKLAQHAELLRKRPATLAAERAHAED"
     gene            1110..2141
                     /gene="dusA"
                     /locus_tag="VM713_11170"
     CDS             1110..2141
                     /gene="dusA"
                     /locus_tag="VM713_11170"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HVH84864.1"
                     /translation="MPDNPTRTLDRRVSVAPMMDWTDRHCRFFLRGFSPDVLLYTEMI
                     TAAAIVRGDRARLLRFNPEEHPLALQLGGSEPHELAAAARAGEEAGYDEINLNCGCPS
                     ERVASGAFGACLMREPARVADCVAKMSAAVRVPVTVKMRIGVVAAAARAARGAVAQYT
                     EEDFEALCGFVQGVTAAGSRVAIVHARQAVLGGLSPRDNREVPPLRYDVVRALKRRFA
                     SLPIVVNGGLRDADAVRAALTWCDGVMLGREAYHRPYVLAELHRALSADTSGPPSRAA
                     LIERMVRYSERELAAGERLGAITRHMLGLYSGEPGARDFRRILSEGARVAGAGAQLLR
                     QALSAAARA"
     gene            2215..3117
                     /locus_tag="VM713_11175"
     CDS             2215..3117
                     /locus_tag="VM713_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVH84865.1"
                     /translation="MGKDIELAILFADVVGSTRLYDTMGDLRARDMVATCIEVMRTAT
                     EQRQGTVIKTMGDEVMATFPTADAALNAAAQMQQQISTHAQLKVEGQPVAIRIGCHFG
                     PVMLENRDVFGAAVHTANRMTSQAKAGQIVTTATTVERLSPEWRASCRQIDVATLKGQ
                     GSEVVLYEVLWQTEDVTSMVPGIAGDARPAHPLRLRLRTEASELLVDERHSSVTIGRA
                     EDNDVVVKGNLISRLHARIEISRNKFVLVDQSTNGTFVQTADGEEAFVRRDSLQLKGH
                     GMIGLGRLPEQGSPQTIRFSCEEA"
     gene            complement(3136..4887)
                     /locus_tag="VM713_11180"
     CDS             complement(3136..4887)
                     /locus_tag="VM713_11180"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HVH84866.1"
                     /translation="MTALSQLSPAVAEWVRSVRELTQPRAVHWVEGTDTEARELTAQL
                     VRSKEFTVLNAQEFPGCHLYRSSPSDVARVEHLTYICSRSQEDAGPNNHWMDPQQAHA
                     RMRELFRGCMRERTLYVIPYCMGPLDSPLSRRGVELTDSPYVVLNMLIMTRAGRAALE
                     LIARDGRFVKGLHSIGELDPERRFIMHFPEELSIESYGSGYGGNALLGKKCHALRIAS
                     WQARSEGWLAEHMLIVGLQSPQGDTHYIACAFPSACGKTNLAMLVPPESMPGWRVLTI
                     GEDIAWLHPGADGRLWAINPEAGCFGVVPGTNPQTSRNAYEMIRRDTLFTNVALTADN
                     QPWWEGLTTGKPVLDWQGRPYDPARGPAAHPNSRFTVAAKRNPSYSAHMEDAAGVPIT
                     AIVFGGRRREVAPLVYEARDWQHGVLVGASLASETTAAAVGQVGVVRRDPMAMQPFCG
                     YNFADYWQHWINTGARLARPPRVFHVNWFRRDAAGKFLWPGYGENLRVLAWMLDRCAG
                     RVGATESAIGRLPLPADLNTRGLDISNEALTALCTIDPAIWRKECGDVREYLGGFGSR
                     LPAALLAELKATESRLG"
BASE COUNT          824 a         1703 c         1815 g          832 t
ORIGIN      
        1 tgaatccggc gaccgaggaa ggtgagcagg tcgagcgtcg cctcatccgc ccaatgcagg
       61 tcctcgagaa tcagcagtgt ttttgccctg ccgagctcgc cgagtgtggc ggcgaacagc
      121 gcggtgggac tcccgtgggc agcgaccgcg gcttgcaacg cgccctgcgt ttgcagcgcg
      181 acgtcatgca gcggggcgag cggacggggc gtgaacagcg catcgcacgc tccccagagg
      241 acgcgtgcct gctcctggcg cttcgcgaat tcccgcaaca gcgcagtctt gccgatgccc
      301 gcttcgccac tcagcagcgc gacgcaaccg ccgtgttgag tagccatgcc gagccatttc
      361 gccaggtcgg cgaagcactg ctctcgttcc aggaggtcca tcgtggcccg gcgcgacgct
      421 agtcttcggc gtgcgcgcgt tccgctgcga gtgttgcggg cctcttgcgc aggagctcgg
      481 catgctgcgc gagcttaggc atccgcgcgg cgagctgttg cgcatcctcg ggataccagc
      541 cggtgagcat gtgcaggtag ggatccacag cggagaactt gtcacccagc acgtaagggg
      601 acagctcggc atggatgcgt tcgaggtgag cgccgtagtc agcgagcgcc tgggccttga
      661 tcgcgggcgc tgcctcggcc ccggaggtgg agtagcgctc ggagtagtag tagcgcagca
      721 ccgcgtcgta gaggctggcg gacataaaca ccatccactg caggaagcgg gcgtgcgcgg
      781 tgctgccggc cgccggagcg agtgcggcgc tcggatgcgc attggtgagg tgaatgagta
      841 tggcggcgga ctccgccacg gccgttccat tcgggagcac caggctcgga atctttccgg
      901 cggggttgac gcggcgcaaa gtctcgagct ccgcagcagt cgtgccgacc cagatcagct
      961 cgtacggggc cccgatctcc tcgaggagaa tctgcggagc gagtgagccc gcaccgcgcc
     1021 ggccgtagag aaggtacttg ccgccagcca tgttgccccc gtcgagctta ctgttgccgc
     1081 attctatctc cccgatcaca ctccctgaaa tgcctgacaa ccccaccaga acattggatc
     1141 gacgcgtctc agtcgctccc atgatggact ggaccgatcg ccactgccgc ttcttcctgc
     1201 gtgggttctc ccccgacgtc ctgctataca ccgagatgat caccgccgcg gcgattgtgc
     1261 gcggcgatcg agcgcggctc ctgcggttca atcctgagga gcatccgctg gctctccagc
     1321 tcggtggcag cgagccgcac gagctcgcgg ccgcggcgcg tgcgggcgag gaggccggtt
     1381 acgacgagat caatctcaac tgtggctgcc cgagcgagcg cgtggcgagc ggcgccttcg
     1441 gggcatgcct gatgcgcgag ccggcgcgtg tggccgactg cgtggcgaaa atgagcgcgg
     1501 cggtgcgcgt gccggtcacc gtgaagatgc gcatcggagt ggtggccgcc gctgcgcgcg
     1561 ctgcccgtgg tgcggtggcg caatacaccg aggaggattt cgaggccctg tgcggcttcg
     1621 tgcagggggt gaccgccgcc ggctcccggg ttgccatcgt gcatgcgcgt caggcggtgt
     1681 tgggaggact ctcgccgcgg gacaaccgcg aggtgccgcc gctgcgctac gacgtggtgc
     1741 gcgctctcaa gcggaggttt gcctcgttgc cgatcgtggt caatggcggg ctgcgcgacg
     1801 cagacgcggt gcgcgcggcg ctcacctggt gcgacggcgt catgctcggc cgcgaagcct
     1861 accaccggcc gtacgtgttg gccgagctgc atcgtgcact cagcgcggat acctcagggc
     1921 cgccgtcacg cgcggcgctc atcgagcgaa tggtgcgcta cagcgagcgc gagctcgccg
     1981 cgggcgagcg cctcggcgcg atcacgcgcc acatgctcgg gctctacagc ggcgagcccg
     2041 gcgcccggga tttccgccgc atcctgagcg agggcgcgcg ggttgcgggg gctggtgcgc
     2101 agctgttgcg acaggcgctg tcggcagcgg cgcgggcctg atgggttcac aaggttggaa
     2161 ttggccttag ttgctagcgg ccccgggacc gtcgcgtata atcatccggc actcatgggc
     2221 aaggacatcg aactcgccat actctttgcc gacgtcgtcg gatcgacccg gctctacgac
     2281 accatgggcg acctgcgcgc gcgcgacatg gtcgccacct gtatcgaggt gatgcgcacc
     2341 gccaccgaac agcgtcaggg cacggtgatc aagaccatgg gcgacgaggt gatggcgacg
     2401 ttcccgaccg ccgacgcggc gctcaacgcc gcggcgcaga tgcagcagca gatctccacc
     2461 cacgcgcagc tcaaggtaga aggccagccg gtcgccatcc gcatcggttg ccacttcggc
     2521 ccggtgatgc tcgagaatcg cgacgtgttc ggagccgccg tgcacaccgc gaaccggatg
     2581 accagccagg cgaaggccgg acagatcgtg accaccgcga caacggtcga gcggctctcg
     2641 cccgagtggc gcgcctcctg tcgccagatc gacgtcgcga ccctcaaggg tcaggggagc
     2701 gaggtggtgc tgtacgaagt gctgtggcag accgaggatg tgaccagcat ggtgccggga
     2761 atcgccggcg atgcgcgccc cgcgcacccg ctgcggctgc ggctgcgcac cgaggccagc
     2821 gagctgttgg tcgacgagcg gcactcgagc gtcacgatcg gccgcgccga ggacaacgac
     2881 gtcgtcgtca agggaaacct catctcgcgg ctgcacgccc gcatcgagat cagccgcaac
     2941 aagttcgtgc tcgtcgacca gagcaccaac ggcaccttcg tgcagaccgc cgatggggaa
     3001 gaggccttcg tgcgccgcga cagcctgcag ctcaagggtc atgggatgat cgggctcggc
     3061 cggctgcccg agcagggctc gccgcagacg atccgcttca gttgcgaaga agcctgacgc
     3121 gcgcgagctg tgtgctcagc cgagccggct ctcggttgcc ttgagctccg cgagcagcgc
     3181 cgccggcaga cggctgccga agccgccgag atattcgcgg acgtcgccgc actccttgcg
     3241 ccatatcgcg ggatcgatgg tgcacagggc ggtgagcgcc tcgttgctga tgtcgaggcc
     3301 ccgggtattg aggtctgccg ggagcggcag tcgaccgatc gcggactcgg ttgcgccgac
     3361 tctgcccgcg cagcgatcga gcatccaggc gagcacgcgc aggttctccc catagcccgg
     3421 ccagaggaac ttgcccgcgg cgtcgcgacg gaaccagttg acatgaaaga cacgcggcgg
     3481 ccgcgccagg cgcgcccccg tgttgatcca atgctgccag tagtcggcga agttatagcc
     3541 gcagaaaggc tgcatcgcca tcgggtcgcg gcgcacgact cccacctggc ccaccgccgc
     3601 agcggtggtt tcggaggcga gcgaggcacc gaccagcacg ccgtgctgcc agtcgcgcgc
     3661 ctcgtagacg agcggcgcga cttcgcggcg ccggccgccg aacacgatgg cggtgatcgg
     3721 cacaccggcg gcgtcctcca tgtgtgccga gtagctcgga ttgcgcttcg cggccacggt
     3781 gaagcgtgag ttcggatgcg cggccggtcc gcgggccgga tcgtacggac gcccctgcca
     3841 gtcgagcacc ggcttacccg tcgtgagtcc ctcccaccag ggctggttgt cggcagtgag
     3901 cgcgacgttg gtgaacagcg tgtcgcgccg gatcatctcg taggcgttgc ggctggtctg
     3961 cggattggtt cccggcacca ccccgaagca gccggcttcc ggattgatgg cccacagccg
     4021 tccgtccgcg ccgggatgga gccacgcgat gtcctcgccg atggtcagaa cccgccagcc
     4081 gggcatggat tccggcggca ccagcatcgc gaggttggtc ttgccgcagg ccgaggggaa
     4141 ggcgcaggcg atgtagtggg tgtcgccctg cggactctgc agcccgacga tcagcatgtg
     4201 ctcggcgagc cagccctcgc tgcgcgcctg ccagctcgcg atgcgcagcg cgtggcactt
     4261 cttgccgagc agcgcattgc cgccgtagcc cgagccgtag ctctcgatgg agagctcttc
     4321 agggaagtgc atgatgaagc gccgctcggg gtcgagctcg ccgatcgagt ggagcccctt
     4381 gacgaagcgg ccgtcgcgcg cgatgagctc gagcgcggcg cgcccggcgc gcgtcatgat
     4441 cagcatgttg agcacgacgt aggggctgtc ggtgagctcg acgccgcggc gggagagcgg
     4501 cgagtcgagc ggacccatgc aatatgggat gacatagagc gtccgctcgc gcatgcagcc
     4561 gcggaacagc tcgcgcatcc gcgcgtgcgc ctgctgcgga tccatccagt ggttgttcgg
     4621 gcccgcgtcc tcctgggagc ggctacatat atatgtgagg tgctcgaccc gcgcgacgtc
     4681 gctgggggat gagcgataga ggtggcagcc ggggaattcc tgcgcgttga gcacggtgaa
     4741 ttccttgctg cgcaccagct gcgcagtgag ctcgcgcgcc tcggtgtcgg tcccctcgac
     4801 ccagtgcacg gcacgcggct gggtcagctc gcgcacggat ctcacccatt cggccaccgc
     4861 gggactcaac tgggacaagg cggtcatctt gtgcgcagga gcggccatcc ggtgatttcc
     4921 tcttctcggg cggtccccta aacccgtaag tatatcccga ccggcctgtg cgccctgcta
     4981 gagtaccggg cggcatcggc agtgccttcg gcgggtcaag gtacgcactc tttgcagcaa
     5041 attcagaagg atatacgtct cgacacctct cccgcggccc gccaggatgt gcggggcgcg
     5101 gtagccccgt agaagcaccc gatgctggac ctcgaggaca ttttcggaca gggaggcccc
     5161 ctggagtgcg ccct
//
LOCUS       DATLKL010000821         4822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_32653, whole genome shotgun sequence.
ACCESSION   DATLKL010000821 DATLKL010000000
VERSION     DATLKL010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32653"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1854)
                     /locus_tag="VM713_11185"
     CDS             complement(58..1854)
                     /locus_tag="VM713_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH84867.1"
                     /translation="MGTINRFKQARTLFLMLPALLAGCASLKPHPAAPPATGDPTALT
                     ITAEAALKRGDCRVAAEDYARAATTGDARLARRATEVALACEHLPAAWEAATRWRTLA
                     PEDRDANALYALVALKLYRTADARVAVRDYCRAALAASTSASTPASAPASTPANTPAD
                     APRRSRPGRDQGVRALGDLIALLLEETDAAAVLTTMSAVPEPTAKEPQALAMFGELAL
                     AAYDGKRAEDFAQQALARDPKDFAALRVLARAQVLRGDATRAIATARSAAAVDAVRGG
                     LELAEVLASLDRVEEAHQQLESLRAGAVPAGEIERRLALLAFQSGDLKEAQRRFAALL
                     TSGEGNEAAQLYLADIAARDGDPERAIAEYRRLYDSSVAVPARSRAAALLLERTARAD
                     SARTEALTLLDDYAAEHPEEELRLTLAKAHLLADHGDADASLKVLATALERHPQHPSI
                     EYDRAVILDQAGHVHESVSALEQLLAQRPDDPSLQNALGYTLADHSLELPRAEGLIRR
                     ALTVMPDSPAALDSLGWVHFRRGDARSAAGTLERAYSIDHDAEIAAHWGEALWVSGVQ
                     GEARKVWAAALAREPQSRPLKATLERLIPDAK"
     gene            1986..3065
                     /gene="prfA"
                     /locus_tag="VM713_11190"
     CDS             1986..3065
                     /gene="prfA"
                     /locus_tag="VM713_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HVH84868.1"
                     /translation="MKDTIRQRLEKLSDRFEEVGRLLATDEVAGGSSQFRDLSMEYAR
                     LAPLAERYREYRGLERDLEAAADMAGDADPGMRALGAEEVARVRGQIGAAEEELRLLL
                     LPKDPRDEKNIFLEVRAGTGGDEAALFAGELYRMYARYAESKGLTVEVLSESPGEHGG
                     YKEIISRIAGKGAFSRLKFESGTHRVQRVPATEAQGRIHTSAVTVAILPEPDEVGEIE
                     IRPAELRIDTYRSSGAGGQHVNKTDSAVRITHLPSGIVVECQDERSQHKNRARAMALL
                     RARLLAAAEEQQQSAQAQSRRLQVGSGDRSERIRTYNFPQGRVTDHRIDLTLYKLAQV
                     MDGQLDELILPLQQEHQAELLAEET"
     gene            3062..3811
                     /locus_tag="VM713_11195"
     CDS             3062..3811
                     /locus_tag="VM713_11195"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HVH84869.1"
                     /translation="MSETAPQLSVDSATGIDVTLPLAGAGARSYAFVVDWHIRLVLAL
                     AWYGAAALLYNYLQNGALSVAPPLTSEARWFGAVLAPALVLYFLYHPLLELIMRGSTP
                     GKRIAGVRVVARDGSAPSAGALLVRNVFRLIDSLPLAYGLGLTLVALTREHVRSGDMA
                     AGTRLVYETAGPAFSVTPATTAGSLDPATAELVAELLARWPRLTVPARRALARQLLER
                     CGRPAEGLAPLDEAALHQALIEATRVPETAA"
     gene            3808..4794
                     /locus_tag="VM713_11200"
     CDS             3808..4794
                     /locus_tag="VM713_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HVH84870.1"
                     /translation="MSSDARQSALAHALMKRAALWRDAEARARRFSHGRSDDPAEAVR
                     VADDYRLLAHDLARARVLLPRSRAREYLESAYARVHATLNHGDWRLGAALEQLFRAEI
                     PAAVRWLAPYLAWTTVLFVLAAAAGYGLVARFPELIALFASPDLIASVESGKLWTEGL
                     LNIAPSSVLSVQILANNIVVSLFAYCSGFLFGLGTLYILGLNGLMLGAIFSFVGQHGL
                     AGALASFIVAHGCVELSVMCLSGAAGAAVGEALVRPGSRGRVAAFRTAALRSGQVLLA
                     CAVLLVGAGLIEGFISPRPGIPLGVKVAVGVGYWLLILALLSGRLWPMRRAS"
BASE COUNT          652 a         1705 c         1754 g          711 t
ORIGIN      
        1 ggcacggtgc ggcagccggc gagcagcagc agcgccgccg ccccagcgcg cgccaggtca
       61 cttcgcatca ggtatgagcc gctcgagcgt cgccttcagc ggtctggact gcggctcgcg
      121 ggcgagcgcc gccgcccaca ccttgcgcgc ctcgccctgc acgccgctca cccacagagc
      181 ctcgccccaa tgcgcggcga tctccgcgtc gtgatcgatc gagtaggcgc gttcgagggt
      241 gccggcggcg ctgcgcgcgt cgccgcggcg gaagtgcacc cagccaagac tgtcgagcgc
      301 ggccggactg tcgggcatca cggtgagcgc gcggcggatg agaccttcgg cgcgcggcag
      361 ctcgagcgag tgatcggcga gggtgtagcc gagcgcattc tgcagcgacg gatcgtcggg
      421 gcgctgggcg agcagctgct cgagggcgct caccgactcg tgcacgtgcc cggcctggtc
      481 gagtatgacc gcgcgatcgt actcgatgga gggatgctgg ggatggcgct cgagtgcggt
      541 ggcgagcacc ttgaggctcg catccgcatc gccatgatcg gcgagcaggt gggccttcgc
      601 gagggtgagc cgtagctcct cctcgggatg ctcggcggcg tagtcatcga gcagcgtcag
      661 cgcctcggtg cgtgcgctgt cggcacgggc ggtgcgctcg agcagcagcg ccgcggcgcg
      721 cgagcgcgcc ggcaccgcga ccgaggaatc gtacagacgg cggtattcgg cgatggcgcg
      781 ctccgggtcg ccgtcgcggg cggcgatgtc ggcgagatag agctgcgcgg cctcgttccc
      841 ctcgccactc gtgagcaacg ccgcgaagcg ccgctgcgcc tccttcagat ctcccgactg
      901 gaatgcgagc agcgcgaggc gccgctcgat ctcgccggcc gggacggcgc ccgcgcgcag
      961 gctctcgagc tgctggtgcg cctcctccac ccgatcgagg gaggcgagaa cctccgccag
     1021 ctcgagcccg ccgcgcaccg cgtccaccgc ggcggcggag cgcgcggtgg cgatcgcccg
     1081 tgtggcatcg ccgcgcagca cctgggcacg tgccaacacc cgcaaggccg caaagtcctt
     1141 cggatcccgc gcgagcgcct gctgcgcaaa atcctcggcg cgcttgccgt cgtaggccgc
     1201 gagcgccagc tcgccgaaca tcgccagcgc ctgcggctcc ttggcggtcg gctccggaac
     1261 ggcgctcatc gtggtgagca ccgccgcggc gtcggtctcc tcgaggagca gcgcgatcag
     1321 gtcaccgagg gcgcgcacgc cctggtcgcg tcccggccgg gagcgccgcg gggcgtccgc
     1381 cggcgtgttc gcgggcgtgc tcgcgggggc gctcgcgggg gtgctcgctg aggtgctcgc
     1441 ggcgagcgcg gcgcgacaat aatcccgaac cgcgacgcgc gcgtcggcgg tgcgatagag
     1501 cttgagcgcc acgagcgcgt agagcgcatt agcatcgcgg tcctcgggcg cgagcgtgcg
     1561 ccagcgggtc gccgcctccc aggcagcggg caggtgctcg caggcgagcg ccacttcggt
     1621 ggcgcggcgg gcgaggcgcg cgtcgccggt ggtggccgcc cgggcgtagt cctcggcggc
     1681 cacccggcaa tccccgcgct tgagtgccgc ctcggcggtg atcgtgaggg ctgtcgggtc
     1741 gcccgtggcc gggggcgctg cggggtgcgg cttgaggctc gcgcagcctg cgaggagcgc
     1801 cggcagcatc aggaacagcg tccgtgcctg cttgaaacga ttgattgtgc ccatcgcgga
     1861 cactatagag ccttcgcccc gggggcgcat tgcgcagctt gtcacaaact gagcacatcc
     1921 cggagaatgc cgcgcgctca gctcgcagca cggctggcgc aggggctttt gcccacgcaa
     1981 cgctcatgaa ggacaccatc cgacaacggc tcgagaagct ctccgatcgc ttcgaggagg
     2041 tcggcaggct gctggccacc gacgaggttg ccggcggctc gtcgcagttt cgcgacctgt
     2101 cgatggagta cgcgcgcctc gcgccgctcg ccgagcgcta ccgtgagtac cggggactcg
     2161 agcgcgacct cgaggccgcc gccgacatgg cgggcgatgc ggatcccggc atgcgcgcgc
     2221 tcggcgccga ggaagtggcg cgcgtgcggg gccagatcgg cgccgcggag gaagagctgc
     2281 ggctgctcct cctgccgaag gacccgcgcg acgagaagaa cattttcctc gaggtgcgcg
     2341 ccgggaccgg gggcgatgag gccgcactgt tcgccggcga gctctaccgc atgtacgccc
     2401 gctacgccga atcgaaaggc ctcacggtcg aggtgctgag cgagagcccg ggcgagcacg
     2461 gcggctacaa ggagatcatc agccgcatcg ccggcaaggg cgcgttctcg aggcttaagt
     2521 tcgagtcggg aacgcaccgc gtgcaacgcg tgcccgcgac cgaggcgcag gggcggatcc
     2581 acacctctgc ggtcaccgtg gcgatcctgc ccgagcccga cgaagtcggc gagatcgaga
     2641 tccgccccgc ggagctgcgc atcgatacct accgctcctc gggcgccggc ggccagcacg
     2701 tcaacaagac cgactcggcc gtgcgcatca cgcacctgcc gagtggcatc gtggtggaat
     2761 gccaggacga gcgttcgcag cacaagaacc gtgcgcgcgc catggcgctg ctgcgggcgc
     2821 gcttgctggc cgccgccgag gagcagcaac agagtgccca ggcccagtcg cgccgcctcc
     2881 aggtcggcag cggcgatcgg tccgagcgca tccgaaccta caacttcccg caggggcgcg
     2941 tcaccgatca ccgtatcgac ctgacgctct acaagctggc gcaggtgatg gacggacagc
     3001 tcgacgagct catcctgccg ctgcagcagg agcaccaggc ggagctgctc gcggaggaga
     3061 cgtgagcgag acggcgccgc agctcagcgt cgacagcgcc accggcatcg acgtcaccct
     3121 gccgctcgcc ggcgccgggg cccgctcgta cgcattcgtg gtggactggc acatccgcct
     3181 ggtactcgcg ctcgcctggt acggcgccgc ggcactcctt tataactatc tgcagaacgg
     3241 cgcgctgtcg gtcgccccac cgctcacgag cgaggcgcgc tggttcggcg ccgtgctggc
     3301 gccggcgttg gtactgtatt tcctctacca cccgctgctc gagctgatca tgcgcggcag
     3361 cacgcccggc aagcgcatcg cgggagtccg cgtggtggcg cgcgacggca gcgccccgag
     3421 cgcgggcgcg ctgctggtgc gcaacgtgtt ccgcctgatc gacagcctgc cgctcgccta
     3481 cgggctgggc ctgacgctcg tggcactcac gcgcgagcac gtgcgcagcg gcgacatggc
     3541 cgcgggcaca cggctggtct acgagaccgc aggccccgcc ttcagcgtca cgccggcgac
     3601 caccgccggc tcgctcgatc ccgccaccgc ggagctggtc gcggagctcc tggcacgctg
     3661 gccgcggctg acggtgccgg cacgccgcgc gctggcgcga cagctcctcg agcgctgcgg
     3721 ccgccccgcc gaggggctcg cgccgctcga tgaggcggcg ctgcatcagg cactcattga
     3781 agctacgcgc gtcccggaga ccgcggcgtg agcagcgatg cccgccagag cgccctcgct
     3841 cacgccctga tgaagcgcgc ggcgctgtgg cgcgacgccg aggcccgtgc ccgccgcttc
     3901 agtcacggcc gctcggacga tccggccgag gccgtgcgcg tggccgacga ctatcggctg
     3961 ctggcgcacg atctggcgcg ggcgcgcgtg ctgctgccgc gctcgcgggc gcgcgagtat
     4021 ctggagagcg cctacgcgcg tgtgcacgcc accctcaatc acggtgactg gcggctcggc
     4081 gccgcgctcg agcagctgtt ccgcgcggag attcccgccg cggtgaggtg gctggcgccg
     4141 tatctggcgt ggaccaccgt actgttcgtg ctggcggccg ccgccggcta cggcctcgtc
     4201 gcccgcttcc ccgagctgat cgcgctgttc gcatctcccg atctgatcgc gagcgtcgag
     4261 agcggcaagc tctggaccga gggcctgctc aacatcgcgc cctcctcggt actgtcggtg
     4321 cagatccttg ccaacaacat cgtcgtgagc ctctttgcct actgcagcgg atttctgttc
     4381 ggtctcggca cgctctatat cctcggcttg aacggcctca tgctgggagc gattttctct
     4441 ttcgtgggcc agcacggtct cgcgggcgcg ctggcttcct tcatcgtggc gcatggctgc
     4501 gtcgagctgt cggtgatgtg cctgtcgggt gccgcgggag ctgcagtcgg cgaggcgctg
     4561 gtgcgcccgg gcagccgcgg tcgggttgcc gcatttcgca ccgcggcgct gcgttcgggc
     4621 caggtgctgc tcgcctgcgc ggtgctgctg gtcggcgccg ggctgatcga aggcttcatt
     4681 tccccccggc cgggcatccc gttgggcgtc aaggtagcgg tcggcgtggg ctactggctg
     4741 ttgatcctgg cgctgctctc ggggcgattg tggcccatgc gccgcgcaag ctagcgctgg
     4801 cgtagccggc aaaaaccacg ct
//
LOCUS       DATLKL010000822         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_77956, whole genome shotgun sequence.
ACCESSION   DATLKL010000822 DATLKL010000000
VERSION     DATLKL010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77956"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VM713_11205"
     CDS             complement(<1..767)
                     /locus_tag="VM713_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HVH84871.1"
                     /translation="MTPPARGRALICGSLAYDTIMVFGDRFANHILPDKIHMLSVSFL
                     VPQLRREYGGCAGNIAYNLALLGDTGYPMATVGRDFGPYYEWMKKSGVPIDHVRTIDA
                     ELTAQAFITTDLDDNQITAFHPGAMQHAHLNQVADARDVSLGIVAPDGREGMIRHAEQ
                     FAAAGIPFIFDPGQGLPMFSGEDLERFVAQATWVTLNDYEWQLAQQKTGWTAGELTRR
                     LAALIVTHGAGGSTIHTREAVFKIPSAPVRAVVDPTG"
     gene            complement(764..1048)
                     /locus_tag="VM713_11210"
     CDS             complement(764..1048)
                     /locus_tag="VM713_11210"
                     /inference="COORDINATES: protein motif:HMM:NF022832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HVH84872.1"
                     /translation="MNIDLLIKMANEIGDFFTGTTDPQQAARDVANHLKRYWDPRMRT
                     QMLAYYEQRQGAGLTDVAKNAVGLLYAAGKAAPGAPPGSIPAGPATGAKA"
     gene            complement(1069..>2039)
                     /gene="fdhD"
                     /locus_tag="VM713_11215"
     CDS             complement(1069..>2039)
                     /gene="fdhD"
                     /locus_tag="VM713_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HVH84873.1"
                     /translation="FFPQPPGASRMRRAGGQGWPPWACAQQGLRSAMDSRRSGKALFA
                     VAPAFVSAEAAAPTASVSAEVSVDRWARGATSHTTDLVAEEMPIALVYHDVPHVVMLA
                     TPADLEDYAVGFTLSEGLVARADEIRGVEVRHGIASVDVHVTVAWERFTQLLQRRRNL
                     TGRTGCGLCGAETAADAIRDTGPVPAGVAVTSAQLHAAIEELSGLQPINARTGSVHAA
                     AWVEPGRGIRVVREDVGRHNALDKTIGALIRGGADLALGFMLITSRASYEMVQKCATV
                     GIPFLAALSAPTAFAVRLAERSGVTLVAFARRDQHVVYAHPGRLRP"
BASE COUNT          305 a          699 c          711 g          324 t
ORIGIN      
        1 cagccggtgg ggtcgacgac cgcgcgcacc ggcgcgctcg ggatcttgaa caccgcctcg
       61 cgcgtgtgaa tcgtggagcc cccagcgccg tgcgtcacga tgagggcggc gagccgccgc
      121 gtcagctcgc cggcggtcca accggtcttc tgctgcgcca gctgccactc gtaatcgttg
      181 agcgtcaccc aggttgcctg ggcgacaaag cgctcgagat cctcgccgct gaacatgggt
      241 agcccctgtc cggggtcgaa gatgaagggg atgcccgcgg cggcaaactg ctcggcgtga
      301 cggatcatgc cttcgcgccc gtcgggagcg acgattccga gggacacgtc gcgggcgtcc
      361 gcgacctggt tcaggtgcgc gtgctgcatc gcgccgggat gaaacgccgt gatctggttg
      421 tcatcgaggt cggtcgtgat gaacgcctgg gcggtgagct ccgcgtcgat cgtgcgcacg
      481 tgatcgatcg gcactccgct cttcttcatc cactcgtaat aggggccgaa gtcgcgcccg
      541 acggtggcca tcgggtagcc ggtgtccccg agcagcgcga ggttgtaggc gatgttaccg
      601 gcgcagccgc cgtactcgcg gcgcagctgc ggcaccagga acgagacgct cagcatgtgg
      661 atcttgtcgg gcaggatgtg attggcgaag cggtcgccga acaccatgat ggtgtcgtag
      721 gcgagcgagc cgcagatcag cgcccggccc cgggccgggg gcgtcacgcc ttggcgccgg
      781 tcgcgggccc tgcggggatc gacccgggcg gcgcgcccgg cgccgccttg ccggccgcgt
      841 acagcagtcc caccgcattc ttcgccacgt cggtgagccc ggcaccctgg cgctgctcgt
      901 agtacgcgag catctgcgtg cgcatgcgtg ggtcccaata gcgcttgagg tgattggcga
      961 cgtcgcgcgc cgcctgctgc gggtcggtcg tgccggtgaa gaaatcgccg atctcgttcg
     1021 ccatcttgat gagcagatcg atgttcatgg atcaggagct cagtgcagtc aggggcgcag
     1081 ccgcccagga tgcgcgtaca cgacatgctg gtcgcgtcgc gcgaaggcga ccagtgtaac
     1141 cccgctgcgc tcggcgaggc gcactgcgaa cgccgtgggc gccgacagcg ccgcgaggaa
     1201 cggaatgccg acggtggcgc acttctgcac catctcgtaa ctcgcgcggc tggtgatcaa
     1261 catgaacccc agcgccagat cggcgccgcc gcgtatcagg gcgccgatcg tcttgtcgag
     1321 cgcgttgtga cggccgacgt cctcgcgtac cacgcgaatg ccccggccgg gctcgaccca
     1381 cgccgcggcg tgcacgctgc cggtacgcgc gttgatcggc tgcagccccg acagctcctc
     1441 gatggcggca tgcagctgcg cgctcgtcac ggccacgccc gctggtacgg gtccggtgtc
     1501 gcgaatggca tcagcggcgg tttccgcccc gcacaagcca cagccggtgc ggccggtgag
     1561 attgcgccgc cgctgcagca gctgggtgaa gcgctcccag gcaaccgtga cgtgcacgtc
     1621 caccgatgcg atgccgtggc gcacctcgac accgcggatt tcgtcggcgc gcgccacgag
     1681 cccttcgctc aacgtgaagc cgaccgcgta gtcctcgaga tcggccggag tcgcaagcat
     1741 caccacgtgc gggacgtcgt ggtagacgag tgcaatcggc atctcctccg cgaccaggtc
     1801 ggtcgtgtga ctggtcgcgc cgcgcgccca gcggtcgacg ctgacctcag cgctgacgct
     1861 cgccgtcggc gctgcggctt cagcgctcac gaatgccggc gcgaccgcaa acagggcttt
     1921 tccggaacgc cgtgaatcca ttgcgctgcg cagtccctgc tgcgcgcaag cccagggcgg
     1981 ccatccctgg ccgcccgctc gccgcatgcg gctcgctcct ggaggctgcg ggaaaaacg
//
LOCUS       DATLKL010000823         1742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_134273, whole genome shotgun sequence.
ACCESSION   DATLKL010000823 DATLKL010000000
VERSION     DATLKL010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134273"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VM713_11220"
     CDS             <1..314
                     /locus_tag="VM713_11220"
                     /inference="COORDINATES: protein motif:HMM:NF023267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HVH84874.1"
                     /translation="CASIVKAAAAAALVVGMAGCTDLKPLQAQVDDLKAQLAKTQSDI
                     SGAKSAADAASSAASSASQAASGAQSTANQALAAAHASQSCCDATNEKIDRMFKRSVS
                     K"
     gene            complement(448..1401)
                     /locus_tag="VM713_11225"
     CDS             complement(448..1401)
                     /locus_tag="VM713_11225"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HVH84875.1"
                     /translation="MHSRIAGTGSHLPQKVVTNFDLEKIVDTSDEWIRTRTGIERRHV
                     AADGETTVDLAEFAARRALDAAGIAPQDVDFIAFGTTTPDLVFPNCGVLLQARLGCRN
                     VPAFSVETACAGFMYALSIADKYVRCGEAQRALVIGAETLSRITDWSDRSTAVLFADG
                     AGAVVLEPSSEPGVLSTHLHADGYYKDLLYCGAGVSKGFGPKLAITMSGNEVFKVAVT
                     KLGQAVDEALAAQGLDRSALDWLVPHQANIRIIQATARKLDLPMERVIVTVQEHGNTS
                     AASVPLALDVAVRDGRIKRGELLLLEAFGGGFTWGSALVRY"
     gene            complement(1401..>1742)
                     /locus_tag="VM713_11230"
     CDS             complement(1401..>1742)
                     /locus_tag="VM713_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HVH84876.1"
                     /translation="VVTDGFTGNVALKTMEGVAGLIADRLRQEFQASFLDRVAGAIAR
                     PVLRRAAAGLDPRRYNGACMVGLTGIVVKSHGSADGVAFSRAIGTAALAARRGLTTHI
                     AQALNAQGAAA"
BASE COUNT          292 a          576 c          603 g          271 t
ORIGIN      
        1 agtgcgcgag cattgtgaaa gctgcggctg ccgcggcgct ggtggtggga atggcaggct
       61 gcacggatct gaagcccctc caggcccagg ttgacgatct gaaggctcag ctggccaaga
      121 cgcagagcga tatatcgggc gcaaagagcg ccgctgacgc ggccagcagc gcggcctcct
      181 ccgccagtca ggcggcgagc ggcgcgcaga gcacggccaa tcaggctctg gctgcggccc
      241 acgccagtca gtcgtgctgt gacgcgacga acgagaagat cgaccggatg ttcaagcggt
      301 cggtatcgaa gtaagcgaag agtctctgga ccgatgaaga acgccgcgct ttgcgcggcg
      361 tttttctatc tgatcatttt ttagcggccg ccctgcggtt gtggggttgg cctgccgaga
      421 gcgccgccgg cgctctcggc ccatagacta ataacgcacc agcgccgagc cccaggtaaa
      481 accaccgccg aaggcttcga ggagcagcag ctcgccgcgt ttgatccgcc cgtcgcgcac
      541 ggcgacatcg agggcgagtg ggaccgaggc cgcggaggta ttgccgtgct cctgcacggt
      601 gacgatcacc cgctccatcg gcaggtcgag cttgcgcgcg gtcgcctgga tgatgcggat
      661 gttggcctgg tgcggcacca gccagtcgag cgcgctgcgg tcgaggccct gcgccgcgag
      721 cgcctcgtcg accgcctggc cgagcttggt caccgcgacc ttgaagacct cgttccccga
      781 catggtaatc gccagcttcg gaccaaagcc cttcgagacc ccggctccgc aataaagcag
      841 atccttgtag tagccgtcgg cgtgcagatg agtggacagc actcccggct cgctgctcgg
      901 ctcgagcacg accgcgcccg cgccgtcggc aaacagcacc gccgtggaac gatcgctcca
      961 gtcggtgatg cgcgagagcg tctccgcgcc gatcaccagc gcacgctgcg cctcgccgca
     1021 gcggacgtac ttgtcggcga tcgacagcgc gtacatgaaa cccgcgcacg ctgtctccac
     1081 gctgaacgcc gggacattgc ggcagccgag gcgcgcctgc agcagcactc cgcagttggg
     1141 gaacaccagg tccggagtag tggtgccgaa ggcgatgaag tcgacgtcct ggggggcgat
     1201 acccgccgca tccagggcgc ggcgcgcggc gaactcggcg agatccaccg tggtctcgcc
     1261 gtcggcggct acgtgccgcc gctcgatgcc ggtgcgcgtg cggatccatt cgtcactggt
     1321 gtcgactatc ttctccaggt cgaagttggt gacgaccttc tgcggcaggt gcgagccggt
     1381 accggcgata cgcgaatgca tcaagctgcg gccccctggg cgttgagtgc ctgcgcgata
     1441 tgggtggtga ggccgcggcg cgccgccagt gccgccgtgc cgatggcgcg cgagaaggcg
     1501 acgccgtcgg cgctgccatg gctctttacc acgattccgg tgagaccgac catgcaggcg
     1561 ccgttgtagc gccgcggatc gaggccggcc gccgcgcggc gcagtaccgg ccgcgcgatc
     1621 gcgccggcga cgcggtcgag gaacgaggcc tggaattcct ggcgcagacg atcggcaatc
     1681 aggcccgcga cgccctccat ggtcttgagc gcgacgttgc cggtgaagcc atcggtgacc
     1741 ac
//
LOCUS       DATLKL010000824         3675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_21200, whole genome shotgun sequence.
ACCESSION   DATLKL010000824 DATLKL010000000
VERSION     DATLKL010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21200"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VM713_11235"
     CDS             <1..584
                     /locus_tag="VM713_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HVH84877.1"
                     /translation="EDPLEFLHTNKRSIVNQREVGLDTKSYARALRGVVRAAPDVILI
                     GEIRDRESMEAAINLAGTGHLVLATLHANNCAESLDRIINMFPREQHNQIFLDLSQYL
                     RAIMAQRLIIGVDNKRLAALEVMINTPHIQTLVKKGDVVAIKEAIAASSERGIQSFDL
                     ALFDLYRAGRVQLEDALANADSRANLEAKINFG"
     gene            complement(614..1504)
                     /locus_tag="VM713_11240"
     CDS             complement(614..1504)
                     /locus_tag="VM713_11240"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HVH84878.1"
                     /translation="MSSSVRGSIFLERARVISQLAYDAEQFVLRVAAPKCAAHAEPGS
                     FAHVTCDEAIPMRRPLSIMRADTRAGWVEFLYKVEGRGLHALASRKAGDAVSCLGPIG
                     RPFHAHPERPRTLLIGGGVGIPPMVFLAERLHQAAAAATAAGAWKPLVLMGSEIPFPF
                     RPRPSTLIVAGIPDGTIAAMPLLDEWGVPSRLASRADFPGCFPGFVTQLADAWLKSLG
                     RQELREVEIFACGPTPMLEVTAQVARRYGVPCQVSLEEFMACAVGGCAGCTVEVRTPA
                     GPAMKRVCVDGPVFDAYTVF"
     gene            complement(1501..2478)
                     /locus_tag="VM713_11245"
     CDS             complement(1501..2478)
                     /locus_tag="VM713_11245"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HVH84879.1"
                     /translation="MTEQLRPDGSLRHLLTLETLSRAQVERLLDRAQTFVRPLGEKPP
                     RSQMLAGLTVANLFTEPSTRTRVSFELAARRLGAEAVNLEVQLSSRVKGESMLDTVYT
                     LQVLHVDALVIRDAEAGVPGLVAEHVAPHVSVLSAGEAHLSHPTQGLLDALTIYQRKQ
                     RFAGLAVAIVGDVRHSRVARSAWHALRVLGVTDLRIVGPAALLPGADEFAGCARYKRL
                     AEGLKGVDVIMMLRIQKERFGDADVPDGDRYFSAWGLTPERLALARPDAIVMHPQPMN
                     RGIEIASEVADGPQSVIRDQVRNGVAVRMAVLAEVLGAPGARVSPSAAP"
     gene            complement(2475..2915)
                     /gene="ruvX"
                     /locus_tag="VM713_11250"
     CDS             complement(2475..2915)
                     /gene="ruvX"
                     /locus_tag="VM713_11250"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HVH84880.1"
                     /translation="MTAVETVLAFDFGLKRIGLASGDTLTRSAAPRRAVAVNSSGPDW
                     VAIEGEVRSLAPARLVVGAPYNADGSEGALSKHARRFAAELERRFALPVHLVDERCSS
                     LEASAQLKARRASGERRRRVRRADVDSAAAAVILGRYFADEGER"
     gene            complement(2955..3605)
                     /locus_tag="VM713_11255"
     CDS             complement(2955..3605)
                     /locus_tag="VM713_11255"
                     /inference="COORDINATES: protein motif:HMM:NF001266.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HVH84881.1"
                     /translation="MVRKQATSGATPPESGSGSDPASGSPVFADGAAGSLTNHLLIAM
                     PQLNDPNFAQTVALICEHTDKGALGIVLNKPLPMKLSEVLSQMKLEPTNEHIGEMPVL
                     RGGPVHTDRGFVLHRPGGQWDHTHKVSDTIQVTTSRDVLAAMARGEGPSDAFIALGYA
                     GWESGQLERELKENAWASMPVDARVVFELPFEERWAGAWRLMGIDVDRLSPEAGHA"
BASE COUNT          575 a         1308 c         1282 g          510 t
ORIGIN      
        1 tcgaggaccc gctcgagttc ctgcacacca acaagcgctc gatcgtcaac cagcgcgagg
       61 tggggctcga caccaagtct tacgcgcgcg cgctgcgcgg cgtggtgcgc gcggcgccgg
      121 atgtgatcct gatcggcgag atccgcgacc gcgagagcat ggaggcggct atcaatctcg
      181 ccggcacggg gcatctggtg ctcgccacgc tgcacgccaa caactgcgcc gaatcgctcg
      241 accgcatcat caacatgttc ccgcgcgagc agcacaacca gatcttcctc gacctgtcgc
      301 agtacctgcg cgccatcatg gcgcagcgcc tgatcatcgg ggtcgacaac aagcggctcg
      361 ccgcgctcga ggtgatgatc aacaccccgc acatccagac gctggtgaag aagggcgacg
      421 tggtggcgat caaggaagcc atcgccgcga gcagcgagcg cggcatccag agcttcgacc
      481 tcgcgctctt cgacctctac cgggccgggc gggtgcagct ggaagatgcg ctcgcgaacg
      541 ccgattcgcg cgccaacctc gaggcgaaga tcaacttcgg ctagctgcag cgcgggccca
      601 gcaggtccgc agctcagaac accgtgtagg cgtcgaacac cggcccgtcg acgcacacgc
      661 gcttcatggc tggtcccgcg ggggtgcgca cctcgaccgt acagcccgcg cagccgccga
      721 ccgcgcacgc catgaactcc tcgagcgaca cctggcaggg cacgccgtag cggcgtgcga
      781 cctgcgcggt cacctcgagc atcggcgtcg gtccgcaggc gaagatctcg acctcgcgga
      841 gctcctgccg gccgagcgac ttgagccagg cgtcggcaag ctgcgtgacg aagcccggga
      901 agcatcccgg gaagtccgcg cggctcgcga ggcggctcgg cacgccccac tcatcgagca
      961 gcggcatcgc ggcgatcgtg ccgtccggga tcccggcaac gatgagcgtc gagggtcgcg
     1021 gtcggaacgg aaacgggatc tcggagccca tgagcaccag cggcttccag gcgcccgcgg
     1081 cggttgcggc ggcggccgcc tggtgcagcc gctcggcgag gaacaccatc ggcggaatgc
     1141 cgacaccgcc cccgatcagc agcgtgcgcg gccgctcggg atgcgcgtgg aagggacggc
     1201 cgatgggacc caggcagctc accgcatcgc ctgccttgcg ggaggcgagc gcgtgcaggc
     1261 cgcgcccctc caccttgtag aggaactcga cccagccggc gcgcgtgtcg gcgcgcatga
     1321 tcgacagcgg ccggcgcatg gggatggcct catcgcaggt cacgtgcgcg aagctgcccg
     1381 gctcggcatg cgcggcgcac ttcggcgccg cgacgcgcag tacgaactgc tcggcgtcgt
     1441 aggccagctg ggagatgacg cgcgcgcgct cgaggaagat gctgccgcgg acgctcgagc
     1501 tcatggcgcc gcgcttgggc tcacgcgcgc gcctggcgcg ccgaggacct cggccagcac
     1561 cgccatgcgc acggcgacgc cgttgcgcac ctggtcgcga atcacggact gcggtccgtc
     1621 ggcgacctcg gaggcgatct cgatgccgcg gttcatcggc tgcggatgca tcacgatggc
     1681 gtcgggccgc gccagtgcga gccgctcggg cgtgaggccc caggcgctga agtaccggtc
     1741 gccgtccggc acgtcggcat cgccgaagcg ctctttctgg atgcgcagca tcatgatgac
     1801 gtccacgccc ttcagcccct cggcgagcct cttgtagcgg gcgcagcccg cgaactcgtc
     1861 cgctccgggc aggagcgctg ccgggccgac gatgcgcaga tccgtcacac cgagcacacg
     1921 cagcgcatgc cacgccgagc gcgccacgcg cgaatggcgc acgtcgccca cgatcgccac
     1981 ggcgagaccc gcgaaacgct gcttgcgctg atagatcgtg agcgcatcga gcaggccctg
     2041 cgtcggatgc gagagatgcg cctcgcccgc cgacagcaca ctgacgtgcg gggcgacgtg
     2101 ctcggcgacc agtcccggga cgccggcctc ggcgtcgcgg atcacgagcg cgtcgacgtg
     2161 cagcacctgc agtgtgtaca ccgtgtcgag catgctctca cccttcaccc gcgaggagag
     2221 ctgcacctcg agattgaccg cctcggcccc gagacgcctg gcggcgagct cgaagctgac
     2281 gcgcgtgcgg gtcgaaggct cggtaaagag gttggcgacc gtgaggccgg cgagcatctg
     2341 actgcgcggc ggcttctcgc cgagcggccg gacgaaggtc tgcgcgcggt cgagcagccg
     2401 ctcgacctgc gcccgcgaca gcgtctcgag cgtcagcagg tgccggagcg acccatcggg
     2461 ccgcagctgt tcagtcatcg ctctccctcg tccgcgaagt agcgcccgag aatcaccgca
     2521 gcggcggcgc tgtcgacgtc cgcgcgcctg acgcgccgcc ggcgctcacc gcttgcgcgc
     2581 cgcgccttga gctgcgcgct cgcctcgaga gaggagcagc gctcatcgac caggtggacg
     2641 ggtagtgcga agcggcgttc gagctcggcg gcgaagcggc gcgcgtgctt gctgagcgcc
     2701 ccctcgctgc cgtcggcatt atacggggcg ccgacgacca gccgcgccgg cgcgagcgag
     2761 cgcacctcgc cctcgatcgc aacccagtcg ggaccggagc tgttgaccgc gaccgcgcgg
     2821 cgcggggcgg cactgcgtgt cagtgtgtcg ccggaggcga ggccgatgcg cttgagtccg
     2881 aagtcgaacg cgaggaccgt ctcgaccgcc gtcatgcccg gtgaggtcgg cgggtgccgc
     2941 agcgctcgcg gcgctcaggc atgacccgcc tcggggctca agcggtcgac gtcgatgccc
     3001 atcagccgcc atgctcccgc ccagcgctcc tcgaagggca gctcgaacac gacccgggca
     3061 tcgaccggca tcgacgccca ggcgttttcc ttcagctcgc gctcgagctg accggactcc
     3121 cagccggcgt agccgagggc gatgaaggca tccgaggggc cttcgccgcg cgccatggcc
     3181 gcgagcacat cgcgcgaggt cgtcacctgg atcgtgtcgg agaccttgtg cgtgtgatcc
     3241 cactgcccgc cgggccgatg cagcacgaag ccgcggtccg tgtgtaccgg gccgccgcgc
     3301 agcaccggca tctcgccgat gtgctcgttc gtgggctcga gcttcatctg cgagagcacc
     3361 tccgagagct tcatcggcag cggcttgttg agcacgatcc cgagcgcgcc cttgtcggtg
     3421 tgctcgcaga tgagcgccac cgtctgcgcg aagttcgggt cgttgagctg cggcatggcg
     3481 atcaggagat gattggtcag gctcccggcg gcgccgtcgg cgaataccgg ggacccgctg
     3541 gcgggatcac tccccgagcc gctttcgggt ggagtggcgc cgctcgtggc ttgcttgcgg
     3601 accatgtccc aagtatgggc acggcagccc cgactctcaa ggaaccgata cggagccgcg
     3661 ctgtacccgc cctcc
//
LOCUS       DATLKL010000825         3037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_122966, whole genome shotgun sequence.
ACCESSION   DATLKL010000825 DATLKL010000000
VERSION     DATLKL010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122966"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1692
                     /locus_tag="VM713_11260"
     CDS             313..1692
                     /locus_tag="VM713_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVH84882.1"
                     /translation="MKDLTQGPITGQLIAMAVPIAIGMLFQTLYLVVDMYFVAHFGEA
                     AIAGVSAAGTVMFVIMALTQMLGVGTVALVSQAVGRKDQAEANVIFNQSVTLSAICGA
                     LTLAAGWLLGSVYVRSMAADAATTAAGTAFLYYYTPGLALQFALVALGSALRGTGIVQ
                     PTMVVQVLTVLLNAALAPVLIAGWGTHHPLGAAGAGLASSISVAAGVVFLWIYFARLE
                     HYVKIDTALLAPRGPVVRRILNVGLPAGGEFFMIFVIIGIIYVVIRGFGPAAQAGFGI
                     GGRVMQALFLPVMALAFAAPAVAGQNFGARQFGRVRETFRSAALLSSVLMLVLTLVCQ
                     WRPQVFIEFFSRQPEVVDVGATYLRVISWNFLATGLVFTCSGLFQALGNTWPSLASSG
                     LRLLTFVLPAVILSRLPHFSLLEVWYLSVASVTLQAITSLALLQREFRRKLGPAPDST
                     LTGINRPAA"
     gene            complement(1758..2579)
                     /locus_tag="VM713_11265"
     CDS             complement(1758..2579)
                     /locus_tag="VM713_11265"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HVH84883.1"
                     /translation="MRKRTALFSLLAGLALVGAAAVVHAQAREEGRLLVASEILKDMR
                     GARDQSIPDRLLERAYGILVIPDTTKIAWFFGGRRGHGVMVVRDKDGKFSSPVFVTMT
                     GGSFGFQWGVQSTDIVLVFTTPRGVQGINGGKITLGGDVSVAAGPVGRDAFAATDAMF
                     KAEVFSYSRSRGAFVGLALDGTVISIDDKADAHFYHKPGVLAGDIMSGSVRSEDESSR
                     RFLGAVSSSTTESRRQASAASAPHHPAAPARKPAPAAGSAQSFPLADSKPGSEPK"
     gene            complement(2729..>3037)
                     /locus_tag="VM713_11270"
     CDS             complement(2729..>3037)
                     /locus_tag="VM713_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84884.1"
                     /translation="TPRRTPASTATCSRTRITPTSWRATELAEFNSWTLGVGASYEFS
                     IPHVRWIHRSTANIRYDHMTVDYKDFRNALLAAQYGAGNEPLYKLDANILEAFLSVWY
                     "
BASE COUNT          431 a          987 c         1084 g          535 t
ORIGIN      
        1 tgtcgttcag cagatggcag aagctgatgg ccgccaggat tgcgactgcg gtgcgacctg
       61 cggtgtgaac gccggcgagg cgcaggacgc tttcgttcaa ggacttgagg ctccgcaggg
      121 cacagccgcg caattttcca cagattgtcg gccgcggcca atctcccgga atgggcggac
      181 cggcggcggt cccggcaggc gcggtacggc gggcggcgag ccgcccgctg cgtgcggcgg
      241 cggccgcgcg ctcacgtggc aagcgcgccc ggcctcatct aatgtggcgg ccagagggag
      301 cgggggagcc agatgaaaga cctgacccag ggcccgatca ccggacagct gatcgcgatg
      361 gccgtgccga ttgcgatcgg catgctgttc cagacgctgt acctggtagt cgacatgtac
      421 ttcgtggcgc acttcgggga ggcggccatc gccggggtca gcgccgccgg cacagtcatg
      481 ttcgtcatca tggccctgac gcagatgctg ggggtgggca cggtcgcgct ggtctcgcag
      541 gccgtggggc gcaaggatca ggccgaggcc aacgtcattt tcaatcagtc ggtcacgctg
      601 tcggcgatct gcggcgcgct gacgctcgcg gcgggatggc tgctggggag cgtgtatgtg
      661 cgctcgatgg cggcagatgc ggcgacgacg gccgcgggca ccgcctttct ctactactac
      721 acgccgggac tcgcgctgca gttcgcgctg gtggcactcg gctcggcgct gcgcggcacg
      781 ggcatcgtgc agccgaccat ggtggtgcag gtgctcaccg tgctgctcaa cgcagcactc
      841 gccccggtgc tgatcgccgg ctggggcact catcaccccc tcggcgccgc cggggcggga
      901 ctggcgagca gcatttccgt cgccgcgggc gtggtgttcc tgtggatcta cttcgcgcgg
      961 ctcgagcact acgtgaagat cgacacggcg ctgctcgcgc cgcgtggccc tgtcgtgcgc
     1021 cgcatcctca acgtgggcct gccggccggc ggcgagttct tcatgatttt cgtgatcatc
     1081 ggcatcattt acgtggtcat ccgcggcttc ggccccgcgg cgcaggccgg tttcggcatc
     1141 ggcgggcggg tgatgcaggc actgttcctg ccggtgatgg cgctcgcgtt cgcggcgccg
     1201 gcggtggccg gacagaactt cggcgcgcgc cagttcggcc gggtgcgcga gaccttccgt
     1261 tcggcggcgc tgctgtcttc ggtactgatg ctggtgctga cgctcgtctg tcagtggcgg
     1321 ccgcaggtct tcatcgagtt cttcagccgc cagcccgagg tggtggacgt cggcgcgacc
     1381 tacctgcggg tcatctcgtg gaatttcctc gccaccgggc tcgtttttac ctgctcgggg
     1441 ctgttccagg cgctcggcaa cacctggccg tcactcgcga gctcggggtt gaggctcctg
     1501 accttcgtgt tgcccgcggt gatcctgtcg aggctgccgc acttctcgct gctcgaggtg
     1561 tggtacctgt cggtggcaag cgtgacgctg caggcgatca cgagcctggc cctcctgcag
     1621 cgcgaattcc gccgcaagct cggtccagcg cccgattcaa ccctcaccgg catcaaccgg
     1681 cctgccgcgt aggcagggcc gggcccgggg gggcccgccc tgacggccgg ctgaggccgc
     1741 tgacttcggc gcgcgcgtta cttcggttcg ctgcccggct tcgagtcggc gagcgggaag
     1801 gactgcgcgc tgcccgcggc cggagcgggc ttccgggccg gcgccgccgg atgatgcggg
     1861 gcggaggccg ccgacgcctg gcgccgactc tcggtggtgc tgcttgagac cgcgccaagg
     1921 aagcgccgcg atgactcgtc ttcgctgcgc acgctccccg acatgatgtc gcccgccagg
     1981 accccgggct tgtgatagaa gtgggcatcg gccttgtcgt cgatcgagat gaccgtgcca
     2041 tcgagcgcca ggccgacgaa ggcgccgcgg ctgcgcgagt aggagaagac ctccgccttg
     2101 aacatcgcgt cggtggcggc gaaggcgtcg cgtccgaccg ggccggccgc taccgagacg
     2161 tcgccgccga gcgtgatctt gccgccgttg attccctgga cgccccgcgg cgtggtgaag
     2221 accagcacga tgtccgtcga ctgcaccccc cactggaagc cgaagctgcc accggtcatc
     2281 gtgacgaaaa cggggctcga gaacttgccg tccttgtcac gcacgaccat cacgccatgt
     2341 ccgcggcgtc cgccgaagaa ccaggcgatc ttggtcgtgt ccggaatgac gaggatcccg
     2401 taggcgcgct cgagcagccg atcggggatg gactgatcgc gcgcgccgcg catgtccttg
     2461 aggatctcgc tcgccaccag gagccgtcct tcctcgcgcg cctgggcgtg gaccaccgcg
     2521 gcggcgccga cgagtgcgag gccggccagc aggctgaaca aagcagtgcg tttgcgcatg
     2581 atgtgatttc ctctccgtgt ggcacgctgt cgcctctcgc cgacaggcat gcgactcaag
     2641 ccatgaacga ccagggcgca gcttaactca gacgcgcacc gagcggcgca tagtcgtgca
     2701 cgcgcagccg gttcagctgc ggttcagctc agtaccagac ggacaggaac gcttcgagga
     2761 tgttggcgtc gagcttgtag agcggctcgt tgccggcgcc gtactgggcc gcgagcagcg
     2821 cgttacggaa gtccttgtag tcgaccgtca tgtggtcgta gcggatgttg gcggtgctgc
     2881 gatggatcca gcggacgtgc ggaatcgaga attcgtacga ggcgccgacg cccagcgtcc
     2941 agctgttgaa ctcggcgagc tcggtcgcgc gccatgaagt tggcgtaatt cgcgtacggg
     3001 aacaggtcgc tgtagaagct ggcgtgcgtc tgggtgt
//
LOCUS       DATLKL010000826         2638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_77986, whole genome shotgun sequence.
ACCESSION   DATLKL010000826 DATLKL010000000
VERSION     DATLKL010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77986"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VM713_11275"
     CDS             <1..255
                     /locus_tag="VM713_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84885.1"
                     /translation="APQDMNSMAPAGDTNQPIASKVPVATSMPPYYYQFTFLTPGSYT
                     VAFTCQAAQDNPAQADAAVKFTPIKSAVVTAGQATTVDFP"
     gene            292..855
                     /locus_tag="VM713_11280"
     CDS             292..855
                     /locus_tag="VM713_11280"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HVH84886.1"
                     /translation="MRQGSAPQVREPRRSRGTAIAVGATLLLVTLGLVWTYRPEAPLP
                     GGTKADLLVVNKSARRMLVYAHGSLLRSYRVSLGHDPQGPKRRQGDHRTPEGRYVIDR
                     HNPDSAYHLSLHVSYPSAADAARARAAGYSPGGEIMIHGTRNGLGWLARVRLPVDWTD
                     GCIAVTNAEMDELYRVVPDGTPIEIRP"
     gene            931..2004
                     /locus_tag="VM713_11285"
     CDS             931..2004
                     /locus_tag="VM713_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84887.1"
                     /translation="MSRWMPRRLSPAAALLLAAAAAALSPPSASAQVQAQPPYAVRVF
                     AYAPANTSQPDSIVAWRDRIIVGFENHVAKDGSDGKSSTIVEFSLSGEVLSSFSVPGH
                     NDGLRVVGGDQLWALQNEDANPNLVVIDLRSGASTPYSFAPTPHGGGYDDIVVGPDGA
                     VYLTASNPNLNAAGINVFPALVQARLGNGNVLLEPVLYGNASATDIATGNTVALNLTD
                     PDSMTRDLKGDIVFTSQADSVLIFVRHPGEGDQTVGQLDISSTATGPGGALITIDDTA
                     FTPPGAEYLLVTDIGGNTIYRVDRSDFGFEPGQAYSASDTAGIVGTLNLDNGVVTPIV
                     TGLVSARGLQFVRARPDGNDSSR"
     gene            complement(2020..2448)
                     /locus_tag="VM713_11290"
     CDS             complement(2020..2448)
                     /locus_tag="VM713_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84888.1"
                     /translation="MARNAALVLAAILVCAGCSTSSHVLIGTARPPISPESVRVYRQP
                     PPKYEEIATINATSQGSLALTSQQNMDKALQRLKQEAARLGANGIVLQGVQDTQSGSI
                     GAGGGNTSYGPNSAVGVGVGGSFAITSKVVQGLAIYVPPQ"
BASE COUNT          422 a          908 c          861 g          447 t
ORIGIN      
        1 gcgcctcagg acatgaacag catggcgccc gcgggggata ccaatcagcc gatcgcctcg
       61 aaggtgccgg tcgcgacctc gatgccgccc tactactacc agttcacgtt ccttacacct
      121 ggcagctaca ccgtggcctt cacctgccag gccgcccagg acaatcccgc tcaggcggac
      181 gcggcggtga agttcactcc catcaagagc gcggtggtta cggcggggca ggccacgaca
      241 gtcgattttc cgtgacggga acctacgaat ttccctgcgc tctactgagc gatgcgtcag
      301 ggaagcgcgc cgcaggtccg cgagccgcgc cggtcgcgag gcacagccat cgcggtcggg
      361 gcgaccctgc tgctcgttac cttaggcttg gtctggacct atcgccccga ggcgcctttg
      421 cccggcggga ccaaggccga tctattggtg gtaaataagt cggcccggcg catgttggtc
      481 tacgctcatg gctcactgct gcgctcgtac cgggtgtcac tcggacacga tccgcaggga
      541 ccaaagcggc gtcagggcga tcaccgtacg cccgaaggcc gctacgtcat cgaccgccac
      601 aatccagaca gcgcctatca cctttccttg cacgtctcgt atccttcggc ggccgacgca
      661 gcgcgcgcgc gtgcggccgg ctactccccc ggcggcgaga tcatgatcca cggcactcgc
      721 aacggccttg gctggctggc tcgcgtgcgc ctgccggtcg actggacgga tggctgcatc
      781 gcggtcacga acgccgagat ggacgagctc tatcgcgtgg tgccggacgg cacgccgatc
      841 gagatccgcc cgtagctctg tgtcacgcga ccgcaacatt ggcggccgac actgcgcctc
      901 cccctgctta gttcgcgcga gggagtcgtc atgagccgtt ggatgccccg ccgcctgtca
      961 cccgccgccg ccctgctgct cgccgctgct gcggcggcac tctccccgcc ctccgccagc
     1021 gcacaggtac aggcgcagcc gccttacgcg gtgcgggtct ttgcgtatgc cccggccaac
     1081 acttcgcagc ccgactccat tgtcgcgtgg cgcgaccgga tcatcgtcgg cttcgagaac
     1141 cacgttgcca aggacgggag cgacgggaag tcgagcacca tcgtcgagtt ctcgctgagc
     1201 ggggaggtgc tgagcagctt cagcgtgccc gggcacaacg acggcctgcg tgtcgtcggt
     1261 ggcgatcagc tgtgggcgct gcagaacgag gacgccaacc ccaatctggt cgtcatcgac
     1321 ctgcgcagcg gcgcctccac gccctatagt tttgcgccca cgccacacgg cggcggctat
     1381 gacgacatcg tggtcgggcc cgacggcgcc gtctatctga cggcctccaa tcccaacctg
     1441 aatgcggccg ggatcaacgt atttccggcg ctggtgcagg cgcggttggg gaacggtaac
     1501 gtgctactgg agcccgtgct ctacggtaac gcctccgcca cggacatcgc gaccggcaac
     1561 acggtggctc tcaacctcac cgatcccgac tcgatgacac gggatctcaa gggcgacatc
     1621 gtcttcacca gccaggccga ctccgtgctg atattcgtgc ggcacccggg cgagggtgat
     1681 cagaccgtcg gtcagctcga catcagctcg accgcgaccg gcccgggcgg ggccctgatc
     1741 accatcgacg acacagcctt tacgcctccg ggcgccgagt atctccttgt cacggacatc
     1801 ggtggcaaca ccatctaccg cgtcgaccgc agcgacttcg gcttcgaacc gggccaggcg
     1861 tactccgcct cggacaccgc gggcatcgtc gggacgctga acctcgacaa cggggtcgtc
     1921 acccccatcg tcaccgggct ggtcagcgcg cgtgggttgc agttcgtacg cgcgcggcct
     1981 gatgggaacg acagctcgcg ctgaggggcg cgccgggcgt tattgcggcg ggacgtagat
     2041 cgcgagtccc tgcaccacct tgctggtgat ggcgaaggag ccgccgaccc cgaccccgac
     2101 cgcgctgtta gggccgtagc tggtgttccc gccgccggcg ccgatcgagc ctgactgcgt
     2161 gtcctgcacg ccctggagca cgatgccgtt ggcgccgagc ctggcggctt cctgtttcag
     2221 ccgctgcagc gccttgtcca tattctgctg gctggtcaga gcgagcgacc cctgactggt
     2281 agcgttgatc gtggcgatct cctcgtattt cggcggcggc tgccgataca cgcgcacgct
     2341 ctcgggggag atcggcgggc gcgccgtgcc gatgagcacg tgggaagaag tcgagcagcc
     2401 ggcgcacacg aggatggcgg cgagcacgag agcggcatta cgcgccatga cgggctcctt
     2461 gagactggca gcatgctatc aaggcggttg gtgaagtaaa tgtccggccc gctgccgctc
     2521 cgggtttaaa ctcgagcccc caaggcaggg tcgcgaccgc cgcggaggtg tgcggatgaa
     2581 attcccgact cgggtgtgcg tggttgccgg tgcgacgctg ctgtggccgg ccgcgcag
//
LOCUS       DATLKL010000827         2912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_145517, whole genome shotgun sequence.
ACCESSION   DATLKL010000827 DATLKL010000000
VERSION     DATLKL010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145517"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..169
                     /locus_tag="VM713_11295"
     CDS             11..169
                     /locus_tag="VM713_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HVH84889.1"
                     /translation="MAAATRILYGGSVKAGNAAELFSMLDVDGGLIGGASLKADEFLT
                     ILAAARPG"
     gene            183..581
                     /gene="secG"
                     /locus_tag="VM713_11300"
     CDS             183..581
                     /gene="secG"
                     /locus_tag="VM713_11300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HVH84890.1"
                     /translation="MLRMLLTAVQVFSSLAIIGLVLLQRGKGAEAGAGFGAGASGTVF
                     GARGATTALSRATAVFAAIFMINSLALAYLGTTTAKEQQKTILEEAAEGGKTSPATGT
                     SPPGSAPAPAPASAPGAAPAATPPAAPPPK"
     gene            615..699
                     /locus_tag="VM713_11305"
     tRNA            615..699
                     /locus_tag="VM713_11305"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:649..651,aa:Leu,seq:gag)
     gene            782..1150
                     /gene="ndhC"
                     /locus_tag="VM713_11310"
     CDS             782..1150
                     /gene="ndhC"
                     /locus_tag="VM713_11310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HVH84891.1"
                     /translation="MLLNYLPILIFLVVAAALAAVLLTLGTGLGRYFARFHSDRAKLS
                     PYECGFEAFEDSRMKFDVRYYLVAILFIVFDLEIAFLFPWAVALGKIGLAGLVAMGIF
                     LAILVVGFIYEWRKGALEWD"
     gene            1180..1635
                     /locus_tag="VM713_11315"
                     /pseudo
     CDS             1180..1635
                     /locus_tag="VM713_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            1641..2354
                     /locus_tag="VM713_11320"
     CDS             1641..2354
                     /locus_tag="VM713_11320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HVH84892.1"
                     /translation="MTGTDQKSESVEALAGRVDARLVASVRRLPAAAHELAYETDGAS
                     LLSVCRTLRDAPDLRFQMLMDLAGVDYLHYGRDDWQTESATRSGFSRARTPQEGPPDP
                     DAPGRFAVVYQLLSITHNWRLRLEVKCPDTAEPAVDSVIDVWASANWFEREAFDLFGI
                     VFRGHPDLRRILTDYGFIGHPFRKDFPLSGNVEVQYDPDRQRVVYRPVSITPRVLVPK
                     VIRHDHRYEPALRDPQVPR"
     gene            2354..>2912
                     /locus_tag="VM713_11325"
     CDS             2354..>2912
                     /locus_tag="VM713_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84893.1"
                     /translation="MAASPLNEIRNYTMNFGPQHPAAHGVLRLVLEMDGEVIRKADPH
                     IGLLHRGTEKLAEFKPFNQSIGYMDRLDYVSMMCNEHAYVLAIERLLGIVPPERAQYI
                     RVMFDEITRVLNHLLWLGSHGLDIGAMTVFLLAFKQREELMDVYEAVSGTRMHATYYR
                     PGGVYRDLPDSMPKYQVTRWHSAKDV"
BASE COUNT          457 a          994 c          957 g          504 t
ORIGIN      
        1 ggatgctaaa attgccgccg ccacgcgcat tctctacggc ggcagtgtca aggccggcaa
       61 cgcggcggag ctcttcagca tgctggacgt cgacggcggc ttgatcggtg gcgcctcgct
      121 caaggccgat gagtttctga caatcctggc cgccgcgcgg ccgggctgag tggatttaag
      181 caatgttacg catgctgctg accgcggttc aggtgttctc ctcgctggcg attatcggcc
      241 tcgtgctgct gcagcggggc aagggagccg aggcgggcgc cggcttcggc gccggcgcgt
      301 cgggaacggt gttcggtgcg cgcggcgcca cgacggcact gtcgcgcgcc acggcggtgt
      361 ttgccgcgat cttcatgatc aacagtctcg cgctcgctta cctcggcacg acgaccgcca
      421 aggagcagca gaagacgatc ctcgaggagg ccgcggaggg tggcaagacg tcaccggcca
      481 cgggcacgtc accgcccggg agtgcgcccg ccccggcccc cgcgtccgcg cctggcgcgg
      541 caccggcggc aactccgcca gccgcgccgc cgccgaagta accgcggcag cggaggcgcg
      601 cattgccgat atttgccgac gtggtggaat tggtagacac actagcttga ggggctagcg
      661 ccgcaaggcg tgtcggttcg aatccgaccg tcggcaccaa aaacgacgac cgcgctgcta
      721 caatgtccag ccttgtcgga ggcaggtcgg agcggtctgc cttttttacg gcccatcgcg
      781 catgctgctg aattacctcc ctatcctgat ctttctggtg gtcgccgccg cgctggccgc
      841 ggttctgctc acgctcggta cgggactcgg gcgctacttc gcgcgcttcc acagcgaccg
      901 cgccaagctc tcgccctacg agtgcggctt cgaggccttc gaagacagcc gcatgaagtt
      961 cgatgtgcgc tactacctgg tggcgatcct gttcatcgtc ttcgatctcg agatcgcgtt
     1021 cctgtttccg tgggcggtgg cgctcgggaa gatcgggctc gccgggctcg tcgcgatggg
     1081 catcttcctc gccatcctcg tcgtcggctt catctacgag tggcgcaagg gagcgctcga
     1141 gtgggactag cggtcgctga aggcccggag accttcgcgc agcgcgggtt tgtcacgacc
     1201 cagctggaca cgctcgtcaa ctgggcgcgg accgggtcac tctggccgat gaccttcgga
     1261 ctcgcctgct gcgccgtcga gatgatgcat gcaggcgctt cgcgctacga cctcgaccgc
     1321 ttcggggtgg tgttccggcc gagtccgcgc cagtccgacg tcatgatcgt cgccggtacg
     1381 ctggtcaaca agatggcccc ggcgctgcgc aaggtgtacg accagatggc tgagccgcgc
     1441 tgggtgatct cgatgggctc gtgcgccaac ggcggcggtt actaccacta ttcctactcg
     1501 gtcgtgcgcg gctgcgaccg catcgtgccg gtcgacgtgt acgtgcccgg ctgcccgccg
     1561 acggccgaag cgctgatcta cggcatcgta cagctgcaga agaagatcgc acgcaccagc
     1621 acgatcgcga gataaggcgg atgaccggga cggaccagaa gagcgagtcg gtcgaggcgc
     1681 tcgcggggag ggtcgacgcg cggctcgtgg cgagcgtgcg gcgcctgccc gcggctgcgc
     1741 acgagctcgc ctacgagacc gacggcgcat cgctcctgtc ggtctgccgg acgctgcgcg
     1801 atgcgccgga cctgaggttc cagatgctca tggatctggc gggcgtcgat tacctccact
     1861 acgggcgcga cgactggcag accgagagcg caacccgctc cggcttcagc cgcgcgcgca
     1921 ccccgcagga gggtccgccc gatccggatg cgcccggccg cttcgccgtc gtctatcagc
     1981 tgctctccat cacccacaac tggcggctgc gtctcgaggt caagtgtccg gacacagccg
     2041 agccggccgt ggattccgtg atcgacgtct gggcgagcgc caactggttc gagcgcgagg
     2101 cctttgacct gttcggcatc gtgttccgcg gccacccgga cctgcggcgc atcctcaccg
     2161 actacggctt catcggccat ccgttccgca aggacttccc gctcagcggc aacgtcgagg
     2221 tgcagtacga ccccgacagg cagcgcgtgg tttaccgtcc ggtgagcatt actccccgcg
     2281 tgctggtgcc caaggtcatc cgccacgacc accgctacga gcccgcgctc cgcgacccgc
     2341 aggtgccacg ctgatggccg ccagcccgct caacgagatc cgcaactaca cgatgaactt
     2401 cgggccgcag cacccggccg cgcacggcgt gctgcgcctg gtgctcgaga tggacggcga
     2461 ggtgatccgc aaagccgatc cccacatcgg gctcctgcac cgcggcaccg agaagctcgc
     2521 cgagttcaag cccttcaacc agtcgatcgg ctacatggac cggctcgact acgtgtcgat
     2581 gatgtgcaac gagcatgcct acgtgctcgc catcgagcgg ctgctcggca tcgtgcctcc
     2641 ggagcgcgcc cagtacatcc gcgtcatgtt cgacgagatc acgcgcgtgc tgaaccacct
     2701 tctgtggctc ggctcgcacg gcctcgatat cggtgcgatg acggtgttcc tgctcgcctt
     2761 caagcagcgc gaagagctca tggatgtcta cgaggcggtg tccggcacgc gcatgcacgc
     2821 cacctactac cgtcccggcg gggtttaccg cgacctgccg gactcgatgc cgaagtacca
     2881 ggtcacgcgc tggcattcgg ccaaggacgt cg
//
LOCUS       DATLKL010000828         1562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_123009, whole genome shotgun sequence.
ACCESSION   DATLKL010000828 DATLKL010000000
VERSION     DATLKL010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123009"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VM713_11330"
     CDS             complement(<1..467)
                     /locus_tag="VM713_11330"
                     /inference="COORDINATES: protein motif:HMM:NF023287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HVH84894.1"
                     /translation="MICEDARRAIGADPDAAGSELEEHLRGCADCRAWREEMQRLNAD
                     LRRALEEPPNLGVPRPRQAPPVWREYALAAGVVLAMAAGLAVWLLLPTDSLARDVVAH
                     VKGEPESWLSTHNVSAGSIDHALRSSGITLDLASDKIVYAQSCWFRGHYVPHLV"
     gene            complement(464..1018)
                     /locus_tag="VM713_11335"
     CDS             complement(464..1018)
                     /locus_tag="VM713_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVH84895.1"
                     /translation="MQSPVSSIGERSRGDDARASLTALFESLRPDLLRFAHWLARDRS
                     IAEDIVQEALLRAWRSRDALKDPAAARAWLLTIVRREHARLYERKRLELVSLDDAVDI
                     PDITDGAAADAQLLTLRAALMKLPIEYREPLVMQVLGGFSAEEIARELELTNTAVLTR
                     LFRARHKLRGLCGPTGDAPGEQPA"
     gene            1150..>1562
                     /locus_tag="VM713_11340"
     CDS             1150..>1562
                     /locus_tag="VM713_11340"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVH84896.1"
                     /translation="MKYLIHWLIDWPESVARHLTWLAPLFARITVGWVFLWSGWGKLH
                     NLPQVTENFVSWGIPFPQLLTPFVSGVEFFGGLFLLAGLFSRISAGALGVTMIVAIRS
                     AKWADVDSLETLLGFDEFEYLALFLWIAIAGAGPVS"
BASE COUNT          232 a          557 c          521 g          252 t
ORIGIN      
        1 accaggtgcg gcacgtagtg cccgcggaac cagcagctct gcgcgtagac gatcttgtcg
       61 gaggcgaggt cgagggtgat gccggagctg cggagtgcgt gatcaatcga gcccgcgctc
      121 acgttgtgag tgctcagcca gctctcgggc tctcccttga cgtgtgcgac cacgtcgcgc
      181 gcgagcgaat cggtcggcag cagcagccac accgcgagcc ccgccgccat cgcgagcacg
      241 actccggccg ccagcgcata ttcccgccac accggcggcg cctggcgcgg acgcggcacg
      301 cccaggttcg ggggctcttc cagcgcgcgg cgcaggtcgg cgttcaggcg ctgcatctcc
      361 tcgcgccagg cacggcagtc cgcgcagccg cgcaggtgct cctcgagctc ggaccccgcc
      421 gcatccggat ccgcgccgat cgcgcgccgt gcgtcctcgc agatcatgcc ggctgctccc
      481 cgggcgcgtc gcccgtgggg ccgcacaggc cgcgcagctt gtgacgtgcc cggaacaggc
      541 gcgtcagcac cgcggtgttg gtgagctcga gctcgcgcgc gatttcctcc gcggagaatc
      601 cccccagcac ctgcattacc agcggctcgc ggtattcgat cggcaacttc atgagggccg
      661 cgcgcagcgt gagcagctgc gcatcggcgg ctgcaccgtc ggtaatgtcg ggaatgtcga
      721 ctgcatcatc gagagacacg agctccagcc gcttgcgttc atagagccgc gcgtgctcgc
      781 gccggacgat ggtcagcagc caggcgcggg cggcggccgg atccttcaga gcgtcccgcg
      841 agcgccaggc gcgcagcagc gcctcctgca cgatgtcctc ggcgatcgac cggtcgcgcg
      901 ccagccagtg cgcgaagcgc agcaggtccg ggcgcagcga ctcgaacagc gccgtgaggg
      961 atgcgcgcgc gtcgtctccg cgagaccgct caccaatgga ggagaccggg gattgcaccg
     1021 ggggattgcg cctgccagca gctgtcaaaa accgccgcga ttatatgtgt ggcgcccggc
     1081 cgcagtggca atatcgcgcc agcccgcccg gtctgtacgc ccaacgctgc actgactcga
     1141 ggagccgccg tgaaatatct catccactgg ctgatcgact ggcccgagag cgtcgccagg
     1201 catctcacct ggctcgcacc gctgtttgcc cgcatcaccg tcggctgggt cttcctctgg
     1261 agcggctggg gcaagctgca caacctgccg caggtcaccg agaacttcgt cagctggggc
     1321 atccccttcc cacaactgct caccccgttc gtgtcgggcg tcgagttctt cggaggactg
     1381 ttcctgctcg ccgggctctt cagccgcatc tccgccggcg cactcggcgt caccatgatc
     1441 gtcgcgatcc gctcggccaa gtgggccgat gtcgactcgc tcgagacgct gctcgggttc
     1501 gacgaattcg agtacctggc gctgttcctg tggatcgcga tcgcaggcgc tgggccggtt
     1561 tc
//
LOCUS       DATLKL010000829         2005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_145529, whole genome shotgun sequence.
ACCESSION   DATLKL010000829 DATLKL010000000
VERSION     DATLKL010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145529"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /gene="selA"
                     /locus_tag="VM713_11345"
     CDS             <1..1443
                     /gene="selA"
                     /locus_tag="VM713_11345"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HVH84897.1"
                     /translation="EAKAAKLPASAPAAGIPSVDRLLHARPFGPLLAQYGRTRVTARL
                     RAHLQGLRRSALGGTLEPAALTDEALAAALAAGLATAAHPALRPVFNLTGTVLHTNLG
                     RARLPEAVSAVTRALSAPVSLELDLESGERGERDTLVEPLLCELTGAQAATVVNNNAA
                     AVLLALSVLASRREVLVSRGELIEIGGSFRLPDIMRRAGAKLVEVGTTNRTHLKDYAE
                     ALGPRASLLLKVHTSNYAISGFTASVPLAKLVKLGRARGVPVAVDLGSGSLVDLEHWQ
                     LPHEPTAREALAAGADLVTFSGDKLLGGPQAGLIVGRADLIARLRQDPLKRALRVSKL
                     TLAALEAVLALYRAPEFLAERLTTLRLLTRAAGDMEAQAVRLAPRVQQAVGPDYDVGA
                     APLVSQIGSGALPVEQLASHGLAIRAAKGRRASLATLEAALRALPRPVLGRLKDKTLW
                     LDLRCLESADEAEFWAQLAALTPASAAPRP"
     gene            1440..>2005
                     /locus_tag="VM713_11350"
     CDS             1440..>2005
                     /locus_tag="VM713_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HVH84898.1"
                     /translation="MIVGTAGHIDHGKTTLVRALTGVDTDRLKEEKARGISIELGYAY
                     TPLPNGDVLGFIDVPGHERLVHTMVAGACGIDFALLVVAADDGLMPQTHEHLAILELL
                     GVRRGAVALTKIDRVDERRRREVEGEVQGTLSATALSDARLFGVNATAAAHPGIEALR
                     QHLEEAAAATPVLSDNRLFRLAVDRVFTL"
BASE COUNT          291 a          730 c          693 g          291 t
ORIGIN      
        1 gaagccaaag cagcgaagct gcccgcgagc gcgcccgcgg ccgggatccc ctcggtcgac
       61 cggctgctgc acgcgcgccc gttcggcccg ctgctcgcgc agtacggacg cacgcgcgtc
      121 acggcgcggt tgcgcgcgca cctgcaggga ctcaggcgta gcgcccttgg cgggactctc
      181 gagcctgccg cgctgaccga cgaggccctg gctgccgcgc ttgcggcggg cctggccacc
      241 gccgcgcacc cggcgctgcg gccggtcttc aatctcaccg gcaccgtgct gcatacgaac
      301 ctcgggcggg cgcggctgcc cgaggccgtg agcgccgtca cgcgcgcgct cagcgcaccc
      361 gtcagtctcg agctcgatct cgagtcgggc gagcgcggcg agcgcgatac gctcgtcgag
      421 ccgctcctgt gcgagctcac gggagcgcag gcggcgacgg tggttaacaa caacgccgcc
      481 gcggtgctcc tcgccctgag cgtgctggcg agccggcgcg aagtgctggt ctcacgcggc
      541 gagctgatcg agatcggtgg ctccttccgc ctgccggaca tcatgcgccg agcgggcgcg
      601 aagctcgtcg aggtggggac taccaatcgg acacatctca aggactatgc ggaagcactc
      661 gggccgcgcg cgtcactcct gctcaaggtt cacacgagca actacgccat ctccggtttc
      721 acggcgagcg tgccgctcgc gaagctggtg aagctcggcc gtgcccgcgg cgtgccggtg
      781 gcagtggatc tcggcagcgg ctcgctcgtc gacctcgagc actggcagct ccctcacgag
      841 cccacggctc gcgaggcgct cgcggccggc gccgacctcg tcaccttcag cggcgacaag
      901 ctcctcgggg gaccacaggc gggcctcatc gtcggccggg ccgatctcat cgcccggttg
      961 cgccaggatc ccttgaagcg cgcgctgcgg gtgagcaagc tcacgctcgc ggcgctcgaa
     1021 gccgtgctgg ctctgtatcg ggctccggaa ttcctcgccg aacgactgac gacgctgcgg
     1081 ctcctgacgc gtgcggcggg cgatatggaa gcccaggccg tgcgcctcgc gccgcgcgtg
     1141 cagcaggcgg tgggtcccga ctatgacgtt ggcgctgccc cgctcgtgag ccagatcggc
     1201 agcggtgctc tccccgtcga gcagctcgcg agccacgggc tcgccatccg cgcggcaaaa
     1261 ggccgccgcg cgagcctcgc cactctcgag gcggcactgc gggcgctgcc gcgcccggtc
     1321 ctcgggcggc tgaaggacaa gactttgtgg ctcgatctgc gctgtctcga gagcgccgac
     1381 gaagccgagt tctgggcaca actcgcggcg ctcacgccgg cgagcgcggc accacgacca
     1441 tgatcgtcgg cacggctggc cacatcgacc acggcaagac cacgctcgtg cgagcgctca
     1501 cgggcgtcga caccgaccgt ctcaaggaag agaaggcccg cgggatctcg atcgagctcg
     1561 gctatgccta cacgccgttg cccaacggag atgtgctcgg cttcatcgac gtgcccggcc
     1621 acgagcgcct ggtgcacacc atggtcgcag gcgcctgcgg tatcgatttc gccctgctgg
     1681 tcgtcgcggc cgacgacggc ctcatgccgc agacgcacga gcatcttgcg atcctcgagc
     1741 tcctcggggt gaggcgaggc gcggtggcgc tcaccaagat cgaccgcgtc gacgagcgac
     1801 gccgccgcga ggtggagggc gaggttcagg gcacgctcag cgccacggcg ttgagcgatg
     1861 cgcgcctctt cggggtgaat gccaccgctg ccgcgcatcc cggcatcgaa gcgctgcgcc
     1921 aacatctcga ggaggcagcg gcggcgacac ccgtgctctc cgacaatcgc ctgttccgtc
     1981 tcgccgtcga tcgcgtattc accct
//
LOCUS       DATLKL010000830         1796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_9927, whole genome shotgun sequence.
ACCESSION   DATLKL010000830 DATLKL010000000
VERSION     DATLKL010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9927"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1505)
                     /locus_tag="VM713_11355"
     CDS             complement(147..1505)
                     /locus_tag="VM713_11355"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HVH84899.1"
                     /translation="MDMFGDWERFLIQVEDSTAHWLAEFLVFANLIQIPAILLTGGVA
                     WLLALPLRREIIELAGDVPAHGRLDWIGRHHAWIVNTAVPLLAPTMWTAGLWSSVAFA
                     QMAGWPQELARIAASLLLAWLVIRLAADLMPYPALAKLIAFVAWALAALNIVNLLGPT
                     IDLMDRAAIQAGSLHLSLLTVVQAALSLGVMLWLSMFISHLLERRITRLPDLTPTAQV
                     LLGKLLKLTLVTLAVVISLASVGIDMSAFAIFTGAIGVVIGFGLQKSISNLFSGIILL
                     LDKSIKPGDVIEVGKTYGWVASLGARYVAVETRDGTEYLIPNEDIITQQVLNWTHQNE
                     LVRLKVPLRTPLDADPHKSIALMKEAARRLPRVLPEPPPNAVVLAFGDSAIELELRFW
                     IRDAKNGIHNIRSEVLLAIWDLFRANGIELPHAKRDVYVRAVPTGVRPVPLQQDLPLA
                     GQ"
BASE COUNT          326 a          594 c          599 g          277 t
ORIGIN      
        1 ctaccccctg ccccagccca agcttcccga caagcccggc gagcagcccc ctaccaccct
       61 ctatcaggcc cccgagcagg gggcttaagc tcgatcctga tcgcgcggac aaggacaggc
      121 gcgcgccgtc gaggcgtgcg ccggccctac tgtcccgcga gcggcaggtc ctgttgcagt
      181 ggcacaggcc tgacgcccgt cggcaccgcg cggacataca cgtcgcgctt ggcgtggggc
      241 agctcgatgc cgttcgcgcg gaacaggtcc cagattgcga gcaggacctc gctccggatg
      301 ttatgaatgc cgttcttggc gtcacggatc cagaagcgga gttcgagctc aatcgcgctg
      361 tcgccaaacg ctagcacgac tgcgttgggc ggcggctcgg gaagcacgcg gggcaggcgc
      421 cgcgccgcct ccttcatcag tgcgatcgat ttgtgggggt cggcgtcgag cggcgtgcgc
      481 agggggactt tgaggcgcac gagctcgttc tggtgggtcc agttcagcac ctgctgggtg
      541 atgatatcct cgttcgggat caggtactcc gtaccatccc tcgtctcgac ggcgacgtag
      601 cgcgcgccga gcgaggcgac ccagccatag gtcttcccca cctcgatcac gtcgcccggc
      661 ttgatcgatt tgtcgagcag aagaatgatc ccgctgaaca ggttcgagat ggatttctgc
      721 aggccgaagc cgatgacgac gccgatggcc ccggtgaaga tggcgaatgc cgacatgtcg
      781 atgccgacgc tcgcgagcga gatcacgacc gcgagagtca ccagggtgag cttgagcagc
      841 tttccgagca gcacctgcgc ggtcggcgtg agatccggca gcctggtaat gcgcctctcg
      901 agcaggtgcg aaatgaacat cgagagccac agcatcacgc ccagcgagag cgcggcctgg
      961 acgacggtga gaagcgacag gtggaggctg ccggcctgga tcgccgcccg atccatgagg
     1021 tcgatggtcg ggcccagcag attaacgatg ttgagcgcgg cgagcgccca ggccacgaac
     1081 gcgatcagct tggcgagcgc gggatagggc atgagatcgg ccgccaggcg gataaccagc
     1141 caggcgagca acaggctcgc cgcgatccgc gccaattctt gaggccagcc cgccatttgg
     1201 gcgaaggcaa ccgagctcca caggcccgct gtccacatgg tcggcgcaag caaggggaca
     1261 gccgtgttca cgatccaggc gtggtgacgt ccgatccagt cgagacggcc gtgggccggg
     1321 acgtcccccg ctagctcgat gatctcgcgc cgcaatggca gcgccagcag ccaggcgacg
     1381 ccgcccgtca gcaggatggc gggaatctgg atgaggttcg cgaagacgag gaactcggcg
     1441 agccaatgcg cggtcgaatc ctcgacctgg atgaggaagc gctcccaatc cccaaacatg
     1501 tccatcgccc cctggccggc gacggctccc cgccccaatc ggctcgcgtt aggaaaccgt
     1561 cccgcgacct cgactagatc ggcctatgct aggagtccgt ccaggaaatg gctataggct
     1621 cagtggcgcc gctcggtccg aaggccgagg gggcgctcgc cggaggacca agcctgctcc
     1681 cgatccagtc gcggtcccac ccccttgtgc ctcgcacaag aaaggctcac ctcgcgctcc
     1741 cgctaatgcc gggcctgggg tgcgcccgcc gccttttgct gggcgtggcg gacatg
//
LOCUS       DATLKL010000831         2617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_157235, whole genome shotgun sequence.
ACCESSION   DATLKL010000831 DATLKL010000000
VERSION     DATLKL010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157235"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VM713_11360"
     CDS             <1..683
                     /locus_tag="VM713_11360"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HVH84900.1"
                     /translation="QIDATLELPDIQRLVHQFRDRKEGEPPRYPAILAELTAAECRQL
                     GETYLEDTRPYRRGAPFFLDKMPNNFRDLGFIHLILPNARVIDARREPMACCFGNFKQ
                     LFPQGMEFKYSLEELGRYYCHYVRLMEHWQRVLPGKVLRVDYEDVVNDLEGSVRRLLD
                     FLDLPFEPACLEFYRTERTVRTLSADQVRRPISREGLEHWRHYEPWLGPLKRALAPLR
                     GRDELMQA"
     gene            complement(669..1196)
                     /locus_tag="VM713_11365"
     CDS             complement(669..1196)
                     /locus_tag="VM713_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84901.1"
                     /translation="MQCALANRRRSVAFIALLLLAAAAESSAQIESCTRLSDDRERLA
                     CFDREVAAQMVRESTGAKAAPAATAVPETPPPKLTDEQKLGLSPARVEQLEKPPGAAA
                     PPKELTVTIRSVAVDGNAHQVFTLTNGQIWRQVELDSAFIVHPEDSVVISRGVGGSYF
                     MSFGKHRSTRVSRLH"
     gene            1290..>2617
                     /locus_tag="VM713_11370"
     CDS             1290..>2617
                     /locus_tag="VM713_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVH84902.1"
                     /translation="MRNTNPALGKAYELEQRGRLDEAIAAYRRLIAFEPENSDALHLL
                     GVALVRASRLQEGLAALSAAARLQPDNPYIQLNRGNALGEFGRDAEAAAAFERAMALK
                     ADLAPAWHAHGRAQLRLKDFAAAERSLSQAARLLPASGGVQCDLGLALERLGRQEEAL
                     ARFERATVLSPGLAEAHHNRGVLQAAGGAPEEALRSLDRALELQPRQAALHTNRGNVL
                     ADLGRTAEALASYDQALTLERHNAPVLRSRGNLLLREQPEKALASFDAALKLEPEDLT
                     THAQRGVALTLLERPAEALISFDRALALKPDSAPVHNGRGVALSNLGRTEEAVVSFAA
                     AVAIAPDHLQALTNGANTLTQLHRHEEALQWFERALAFRPEDPELEWGKSRLLLSRGD
                     FRQGWQLYEARLQLRQLRALQRHSDLPRWLPGQPIEGRTLLVHAEQGLGDT"
BASE COUNT          418 a          900 c          898 g          401 t
ORIGIN      
        1 cgcagatcga cgccaccctc gagctgccgg acattcagcg cctggtgcac cagttccgcg
       61 accgcaaaga gggcgagcca ccccgctatc cggcgattct cgccgagctc acagcggcgg
      121 agtgccgcca gctgggcgag acctacctcg aggacacgcg gccgtaccgc cgcggcgcgc
      181 cgttcttcct cgataagatg cccaacaatt tccgcgatct cggcttcatc cacctgatcc
      241 tgccgaacgc tcgcgtcatc gatgcgcgcc gcgagccgat ggcctgctgc ttcggcaatt
      301 tcaagcagct gttcccccag ggcatggaat tcaaatacag cctggaggag ctcgggcgct
      361 actactgcca ctatgtgagg ctgatggagc attggcagcg ggtgctgccg ggcaaggtcc
      421 tgcgagtgga ttacgaggac gtggtgaacg atctggaggg cagtgtgcgc cgcctgctcg
      481 atttcctcga tctgccgttc gagcccgcat gcctcgagtt ctatcgcacc gagcgcaccg
      541 tgcgcacgct gagcgcggat caggtgcgcc ggcccatcag tcgcgagggc ctcgagcatt
      601 ggcggcacta cgagccgtgg ctgggtcccc tgaagagggc gctcgcgccc ctgcgcggcc
      661 gcgacgagct aatgcaagcg tgacacgcgg gtactgcgat gcttgccgaa tgacatgaag
      721 taggagcctc ccaccccgcg actgatgacc acactgtcct cgggatggac gatgaaagcg
      781 ctatcgagct ccacctggcg ccagatctga ccattcgtga gcgtgaatac ctggtgggca
      841 ttgccgtcca cggcgacgct ccgtatcgtc acagtgagct ccttgggggg cgcggcagcg
      901 cccggcggct tctccagctg ctcgacgcgc gcgggcgaga gccccagctt ctgctcgtcg
      961 gtgagcttcg gtggcggtgt ctcgggtacc gctgtcgccg ccggcgccgc ctttgccccg
     1021 gtggattcgc gtaccatctg cgcggccacc tcgcgatcga agcaggcgag ccgctcgcga
     1081 tcatcggaga ggcgggtaca actctcgatc tgggcgctcg attccgcggc cgccgcgagc
     1141 agcagcagtg ctatgaatgc gacggaccgg cgacgattgg ctagtgcgca ctgcaatttg
     1201 tatcttccgg gtcccggtag ccgtgcccgc ggtcattata tggcgagctg gcggcggcgc
     1261 agcgccgtac gcatactgcg gattcattca tgaggaacac caacccggcg ctcggcaagg
     1321 cctacgagct cgagcagcgc ggacgactcg acgaggcgat cgccgcctat cggcggctca
     1381 tcgccttcga gcccgagaac agcgatgcgc tgcacctgct cggcgtcgcg ctggtgcgcg
     1441 cctcgcggtt gcaggagggg ctcgcggcgc tgtccgccgc ggcgcggctg cagcccgaca
     1501 acccctatat ccaactcaac cgcggcaatg cgctcggcga gttcgggcgc gacgccgagg
     1561 cggcggcggc gttcgaacgc gccatggcgc tcaaggccga cctcgcaccg gcctggcatg
     1621 cgcacggtcg cgcgcagctg cgccttaagg atttcgcggc cgccgaacgc agcctctcgc
     1681 aagccgcgcg gctgctgcct gcgagcggtg gcgtgcagtg cgatctcggc ctggcgctcg
     1741 agcggctggg acgccaggaa gaagcgctag cccgtttcga gcgcgccacg gtgctcagcc
     1801 cgggtctcgc cgaggcgcac cacaaccgcg gcgtgctgca agccgcggga ggtgcgccgg
     1861 aggaggcact caggagcctc gatcgggcac tcgagctgca gccgcgccag gcggcgctgc
     1921 acaccaaccg cggcaacgtg ctggccgacc tcggtcgtac cgcagaggcg ctcgccagct
     1981 acgatcaggc gctcacgctc gagcggcaca acgcgcccgt gctgcgcagc cgcggtaacc
     2041 tgctgctgcg cgagcagccc gagaaggcgc tggcgagctt cgacgccgca ctgaaacttg
     2101 agcccgagga cttgaccacc catgctcagc gcggggtggc gctcacgctg ctcgagcgcc
     2161 cggcggaggc gctgatcagc ttcgaccggg cgctcgcgct caagccggac tccgcgccgg
     2221 tgcacaacgg ccgcggcgtg gcgctcagca atcttggtcg cactgaggaa gcagtggtga
     2281 gtttcgcggc ggccgtggcc attgcaccgg accacctgca ggcactcacc aacggggcca
     2341 acacgctcac gcagctgcac cgccacgagg aggcgctgca gtggttcgag cgagcgctcg
     2401 cctttcggcc ggaggacccg gagctcgagt ggggcaagag ccgcctgctg ctgagccgcg
     2461 gtgacttccg gcagggctgg cagctctacg aagcgcgcct gcagctgcgg cagctgcggg
     2521 cattgcagcg gcactcggac ctgccgcgct ggctgccggg acagccgatc gagggccgga
     2581 cgctgctggt acatgcggag cagggcctcg gcgatac
//
LOCUS       DATLKL010000832         1709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_134397, whole genome shotgun sequence.
ACCESSION   DATLKL010000832 DATLKL010000000
VERSION     DATLKL010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134397"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VM713_11375"
     CDS             <1..625
                     /locus_tag="VM713_11375"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HVH84903.1"
                     /translation="SAKAMRLAGAVALVTAAVLGSGGARADQQLDPGLRDVVARAIAQ
                     AQCFTDQYDSAVWYKLMEPRLRSIVKEHTERLEILREVYCETHRPGEARLPPGLVMAL
                     MAVESHFDRWAVSGAGAVGLMQVMPFWPERLGMRSYELVHVAPNVHMGCAILRYYLGY
                     EHDDVRKALARYNGSPGKRDYPDRVLTAWRRWSGADDLGLPATATKR"
     gene            complement(622..>1709)
                     /locus_tag="VM713_11380"
     CDS             complement(622..>1709)
                     /locus_tag="VM713_11380"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVH84904.1"
                     /translation="TLERIAREGPRGFYAGSTARAIVEATHQEQLAGTMTLQDLASYR
                     PEWDQPLCRPFRHYSVCAPPPPAGGVSLLELLGMLEKTDIATRGPNDPHAWFMFAQAS
                     RLAYADRDRYVADPHFVAVPVERLLDPAYLRLRAQLIGEHAGPAPLPGEIALPRGRDA
                     TTESQGTSHFVAVDSDGNVVSMTTTIESVFGSGRVVGGFVLNNQLTDFSFIPSDDGQP
                     IANAVHGGKRPRSSMAPVIVLDHDGNFVAALGSPGGSAITEYNAKVLVGLLAWKLTMK
                     QAIELPNLIARSDSYTGELAKFTPEVLAGLRARGMVLKLGRAENSGLQGIVRLPDGTY
                     QGAADSRREGVALMLAPRPAKHATATH"
BASE COUNT          261 a          577 c          612 g          259 t
ORIGIN      
        1 ctcggctaag gcgatgcggc tcgcgggcgc cgtcgcgctc gtgaccgccg cggtgctcgg
       61 cagcggcggc gcgcgcgccg atcagcagct cgatccgggg ctgcgcgacg tggttgcgcg
      121 cgccatcgcc caggcgcagt gcttcaccga ccagtacgat tcggcggtct ggtacaagct
      181 catggagccg cggctgcgct ccatcgtgaa ggagcacacc gagcggctcg agatcctcag
      241 ggaggtctat tgcgagacgc accgccccgg cgaagcacgc ctgccgccgg ggctggtgat
      301 ggcgctgatg gccgtcgaga gtcacttcga tcgctgggcg gtctccgggg ccggggcggt
      361 cggcctgatg caggtgatgc ccttctggcc ggagcggctc ggcatgcgca gttacgagct
      421 ggtgcacgtc gcgccgaatg tgcacatggg ctgcgcaatc ctgcgttact acctcggcta
      481 cgagcacgac gacgtgcgca aggcgctcgc gcgctacaac ggcagcccgg gaaagcgcga
      541 ctatcctgac cgcgtgctca ccgcctggag gcgctggagc ggcgcggacg atctgggact
      601 ccctgctacc gccaccaagc gctagtgcgt cgcggtcgca tgcttcgccg gccgcggcgc
      661 cagcatcagc gccacaccct cgcgacgcga atcggccgcc ccctggtagg tgccgtcggg
      721 caggcgcacg atgccctgca gccccgagtt ctccgcccgg ccgagcttca acaccatgcc
      781 gcgcgcgcgc agacccgcga gcacttcggg tgtgaacttg gcgagctccc cggtgtagct
      841 gtcgctgcgc gcgatcagat tgggcagctc gatcgcctgc ttcatcgtca gcttccaggc
      901 gaggagtccg acgagcacct tggcgttgta ctcggtgatg gccgagcctc ccggagagcc
      961 gagtgccgca acgaagttgc cgtcgtgatc gagcacgatc accggcgcca tcgaggagcg
     1021 cgggcgcttg ccgccgtgca ctgcgttggc gatcggctgc ccgtcgtcgc tcgggatgaa
     1081 cgagaaatcc gtgagctggt tgttgagcac gaagccgccg acgacgcgtc cggagccgaa
     1141 caccgactcg atggtggtag tcatcgacac cacgttgccg tctgagtcca cggcgacgaa
     1201 gtgactggtc ccctgcgact ccgtggtggc gtcccggccg cgcggcagcg cgatctcgcc
     1261 cgggagcggc gccgggccgg cgtgctcgcc gatcagctgg gcgcgtaagc gcaggtacgc
     1321 gggatcgagc agccgctcga ccggcacggc gacgaaatgc gggtcggcca catagcggtc
     1381 gcggtcggca taagcgaggc ggctcgcctg cgcgaacata aaccaggcgt ggggatcatt
     1441 agggccgcgt gtggcgatgt cggtcttctc cagcatgcca agcagctcga gcagagacac
     1501 gccgccggcg ggcggcggcg gcgcgcacac cgagtaatgc cggaacggac gacacagagg
     1561 ctggtcccat tccggacgat aggaggcgag gtcctgcagc gtcatcgttc ccgccagctg
     1621 ctcctggtgc gtggcctcga cgatggcgcg cgcggttgac ccggcgtaga agccgcgcgg
     1681 tccctcgcgc gcgatgcgct cgagagtct
//
LOCUS       DATLKL010000833         1500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_55226, whole genome shotgun sequence.
ACCESSION   DATLKL010000833 DATLKL010000000
VERSION     DATLKL010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55226"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="VM713_11385"
     CDS             <1..1419
                     /locus_tag="VM713_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVH84905.1"
                     /translation="RLSLGWVHLTLLGIGGIIGTGIFVLTGTAAANHAGPALPLSFIV
                     AGLGCTFAGLCYAEFAAMIPVSGSAYSYSYATLGEGIAWFIGWNLVLEYLFAVATVSV
                     GWSGYAVSLLQQLHISLPAALTNAPFGTPAGKETFEIVATGAIINVPAILIVAVIATI
                     CYIGIKQSAWFNSVVVAIKVTVILLFIMVGVGYIDTANWHPFVPPNGGQFGLFGWSGV
                     MASSGVIFFAYIGFDAISTAAQETKNPQRDMPIGILASLVVCTILYVIVSAVLTGMVS
                     YKDLDVAAPVALALDRHNAPWAIGLAVKVGAVCGMTSVMLVMTIAQARIFFAMARDGL
                     LPPFFGRVHPRFKTPSVGTAVTGACAALIGGLFPVKVLGEMVAIGTLAAFVTVCIGVL
                     VLRRSRPDLPRPFRAPWPWFTCIAGAVVCGAMMVSLGPATWARLVIWTGIGVLIYAFY
                     GYQHSCLRKANGGAAAATAPSR"
BASE COUNT          201 a          510 c          490 g          299 t
ORIGIN      
        1 cggctgtcgc tcggctgggt gcacctgacg ttgctcggca tcggcggcat catcggcacc
       61 gggattttcg tgctgaccgg cacggcggcc gcgaaccacg cgggccccgc gctgccgcta
      121 tcattcatcg tcgcgggact cggctgcacc ttcgcgggcc tgtgctacgc ggagttcgcc
      181 gccatgatcc cggtctcggg cagcgcctac tcctattcct acgcgaccct aggcgagggc
      241 attgcctggt tcatcggctg gaacctggtg ctcgagtacc tgttcgcagt cgccaccgtg
      301 tcggttggct ggtcgggcta cgcggtcagc ctgctgcagc agctgcatat cagcctgccg
      361 gcggcgctga ccaatgcgcc gttcggaacg ccggcgggca aggagacctt cgagatcgtc
      421 gccaccggag cgatcatcaa cgtgccggcg atactcatcg tcgcggtcat tgccacgatc
      481 tgctacatcg gcatcaagca atcagcctgg ttcaactcgg tggtcgtcgc gatcaaggtg
      541 acggtgatcc tgctgttcat catggtcggc gtgggctaca tcgacaccgc caactggcac
      601 ccgttcgtac ctccgaacgg cgggcagttc ggcctgttcg gctggagcgg cgtgatggca
      661 tcctccggtg tcatcttttt cgcctacata ggcttcgacg cgatctcgac cgccgcgcag
      721 gagaccaaga atccgcagcg cgacatgccg atcgggattc tcgcgagcct ggtggtctgc
      781 accatccttt acgtgatcgt ctccgcggtg ctcaccggca tggtgagcta caaggatctg
      841 gatgtcgccg cgcccgtcgc gctcgccctc gatcgccata acgctccctg ggcaataggc
      901 ctggcggtaa aggtcggtgc ggtctgcggc atgacctcgg tgatgctggt gatgaccatc
      961 gcccaggcgc gcatcttctt cgccatggcg cgtgatggcc tgctgccgcc ctttttcggc
     1021 cgggtgcacc cgcgcttcaa gacaccctcg gtcggcacgg cggtgaccgg agcgtgcgcg
     1081 gcgctcattg gcggcctctt tcccgtgaag gtcctgggcg agatggtggc catcggcacc
     1141 ctggcggcct tcgtcacggt gtgcatcggc gtgctcgtgc tgcgacggag ccgccctgat
     1201 ctgccgcgcc cgttccgcgc cccctggcca tggttcacct gcatcgccgg cgcggtggtg
     1261 tgcggcgcga tgatggtatc gctcgggcct gcgacctggg cacggctggt gatctggacc
     1321 ggaatcggcg tgctgatcta cgccttctac ggctaccagc acagctgcct gcgcaaggcg
     1381 aacggcggcg cggccgccgc gactgctccc tcgcgctagc gaccgctccc gttagcccac
     1441 gcgtcatccc gccgcgcgtt gcggcgttat gaccgtgcac tgggtttctc ggggcggccg
//
LOCUS       DATLKL010000834         2948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_224037, whole genome shotgun sequence.
ACCESSION   DATLKL010000834 DATLKL010000000
VERSION     DATLKL010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224037"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VM713_11390"
     CDS             <1..243
                     /locus_tag="VM713_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84906.1"
                     /translation="ECVLLMAQISRAQRLLPARDALAEEIRAHTERLQPAASYRGPAD
                     TCPTSESIDAARASADSKVPNVLTEDTWDLFRVLLR"
     gene            complement(244..2361)
                     /locus_tag="VM713_11395"
     CDS             complement(244..2361)
                     /locus_tag="VM713_11395"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HVH84907.1"
                     /translation="MTESMRRSSGWITACLVALAALALAGSVQAPASPSLLPPGAIAP
                     TDTQRATARKIGRILEEAHYSHAPIDHRMSDIVFQRYIEFLDPQRSYLLASDVNEFST
                     YRYQFDDMIRTGNIDPAYLMFARFQQRNRERLQYALALLKTEPDWTIDESYEFDRTHA
                     PWPADQAQIDDLWRRRVKNDTLSLLLTGKQWSEAADILRKRYERVLKRIDQVTSEDVF
                     ENLMNAYARAFDPHSSYFSPRSSEEYRIQMSLNYDGIGASLQVVDDYVTIMNIIEGGP
                     AAVAGTLKTNDRITAVGQGHEGVLTDVIGWRLDDVVQLIRGKAGTSVRLEILPGGAAP
                     GSAEKVLEFVRNKITLEAQAAHKDVKTVARNGRTYRIGVITVPGFYQDIAAQNAGDAN
                     YRSTTRDVARLLHELKSENVDGLVLDLRGDGGGYLPEATALTGLFINHGPVVQLRDTS
                     GRLEVLDDPESAPTYDGPLAVLVDRMSASASEIFAGAIQDYHRGLIIGQTTFGKGTVQ
                     NLVPLDRWSKEPVNGQLTVTIGKFYRVTGESTQHRGVQPDIPLASVLDTKEVGESALE
                     SSLPWDRIAGVPFKTSTAAANVPTLATLASAEDARAQRDPDYRWLVSDIAAIDTLREQ
                     HSVSLNLKLRREERAREDRERLERENSRRAAKNLPPLKSVAELDKAKEDQPDVMLDQA
                     AQIMTDVVTGVRPAPVQKTARAG"
     gene            2453..2731
                     /locus_tag="VM713_11400"
     CDS             2453..2731
                     /locus_tag="VM713_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84908.1"
                     /translation="MTALLAILAGSALALATGLIMTWIVILLLPEDEQRFAPEHAHLV
                     RAILVFALFAGVTGASFYGQLRERRWRGAAHGATLAVFALVVWLYWPT"
     gene            complement(2716..>2948)
                     /locus_tag="VM713_11405"
     CDS             complement(2716..>2948)
                     /locus_tag="VM713_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84909.1"
                     /translation="ILGMPFLIWAGGNRVLGPYAHGDNPKAGPWALFADYVVGLAHGS
                     AVFWVVALGPLALVLLIRGFLAFLRWVSQVGQ"
BASE COUNT          444 a          955 c         1028 g          521 t
ORIGIN      
        1 gaatgcgtcc tgctgatggc gcagatcagc cgagcacagc ggctgctccc ggcgcgtgac
       61 gcgctggccg aggagatccg cgcgcatacc gagaggctac agccggcggc gagctatcgc
      121 ggtccggccg acacctgtcc gaccagcgag tcgatcgacg ccgcgcgcgc cagcgcggac
      181 agcaaggtcc ccaacgtgct caccgaggat acttgggacc tgttccgggt attgctgagg
      241 taactacccg gcgcgcgcgg tcttctgcac cggcgcgggc cgcacaccgg tcaccacgtc
      301 ggtcatgatc tgtgccgcct gatcgagcat gacgtcaggc tggtcttcct tggccttgtc
      361 gagctcggcg acgctcttca agggcggcag gtttttcgcg gcgcggcggc tgttctcgcg
      421 ctcgagccgc tcgcggtcct cgcgcgcgcg ctcctcgcgc cgcagcttga ggttgagcga
      481 cacgctgtgc tgctcgcgca gggtgtcgat ggcggcgatg tccgagacca gccagcggta
      541 gtcggggtca cgctgcgcgc gcgcatcctc cgccgaggcg agggttgcca gggtcggcac
      601 gttcgcggcg gccgtgctgg tcttgaatgg cactccggca atacggtccc acgggagcga
      661 ggactcgagc gcgctctcgc cgacttcctt ggtatcgagc accgaggcga gcggaatatc
      721 gggctgcacc ccccgatgct gggtgctctc gccggtcacg cggtagaact tgccgatcgt
      781 cacggtcagt tggccgttca cgggctcctt agaccagcgg tcgagcggca ccagattctg
      841 caccgtgccc ttgccgaagg tggtttggcc gatgatcagg ccgcggtgat agtcctggat
      901 cgcgccggcg aagatctccg aggccgaggc gctcatccgg tcgaccagca ccgcgagtgg
      961 gccgtcgtag gtgggggcgc tctccgggtc gtcgagtacc tcgagccttc ctgaggtatc
     1021 gcgcagctgc accaccgggc cgtggttgat gaagagcccg gtgagggcgg tcgcctccgg
     1081 caggtagccg ccgccgtcgc cgcgcagatc gagcaccaga ccgtcgacgt tctcgctctt
     1141 tagctcgtgc agcaggcggg cgacgtcgcg ggtggtgctg cggtagttcg catccccggc
     1201 gttctgagcg gcgatgtcct gatagaagcc gggcaccgtg atgacgccga tgcggtaggt
     1261 gcggccgttg cgcgccacgg tcttgacgtc cttgtgcgcg gcctgagcct cgagcgtgat
     1321 cttgttgcgc acgaactcca gcactttctc cgcactgccg ggcgccgcgc cgccgggcag
     1381 gatctccagg cgcacgctcg tgccggcctt gccgcggatc agctgcacga cgtcatcaag
     1441 ccgccagcca atgacgtcgg tgagcacacc ctcgtgcccc tggccgacgg cggtgatgcg
     1501 gtcgttggtc ttgagggtgc cggcgacggc agccgggccg ccctcgatga tgttcatgat
     1561 ggtgacgtag tcatcgacca cctggagcga ggcgccgatg ccgtcgtagt tgaggctcat
     1621 ctggatgcga tattcctcgg agctgcgcgg cgagaagtag ctcgagtgcg ggtcgaaggc
     1681 gcgggcgtag gcgttcatca ggttctcgaa cacgtcctcg ctcgtcacct ggtcgatgcg
     1741 cttcagcacc cgctcgtagc gcttgcgcag gatgtcggcg gcctccgacc actgcttgcc
     1801 ggtgagcagc agcgacagcg tgtcgttctt gacgcgccgt ctccacaggt cgtcgatctg
     1861 cgcctgatcc gccggccacg gcgcatgcgt gcggtcgaac tcgtacgact cgtcgatcgt
     1921 ccagtcgggc tcggtcttca gcagcgcgag cgcgtactgc aggcgctcgc gattgcgctg
     1981 ctggaagcgc gcgaacatga ggtacgccgg atcgatgttg ccggtgcgta tcatgtcgtc
     2041 gaactggtag cggtaggttg agaattcgtt gacgtcggag gccagcaggt aactgcgctg
     2101 cggatcgagg aactcgatat agcgctggaa cacgatgtcc gacatccggt ggtcgatcgg
     2161 cgcgtggctg tagtgcgcct cctcgaggat gcggccgatc ttgcgcgccg tggcgcgctg
     2221 cgtgtcggtc ggagcgatcg ccccgggcgg caggagcgaa gggctggccg gggcctgtac
     2281 ggagccggcg agcgcgagcg ccgccagtgc caccaggcac gcagtgatcc agccggagga
     2341 acggcgcatg gattcggtca tcgggtcagc tcacaggttc ggtaaaatct tagagctagg
     2401 ctagagccta ccgttcgtct ttagcaagtg cggtgctcac agcgtgaagc ctttgacggc
     2461 actgctcgcg attctggccg ggtcggccct ggccctggcg accgggctga tcatgacctg
     2521 gatcgtcatc ctgctgcttc ccgaggacga acagcgcttc gctcccgagc atgcccacct
     2581 ggtccgcgcc atcctggtct tcgcgctgtt tgcgggggtt acgggcgcga gcttctatgg
     2641 gcagttgcgt gagcggcgct ggcggggggc ggcgcacggc gccacgctcg cggtattcgc
     2701 cctggtggtc tggctctact ggcccacctg agacacccag cgcaggaacg ccagaaaacc
     2761 gcggatcagc agcaccagcg ccaggggacc cagcgccacg acccaaaaca ccgccgagcc
     2821 gtgggccaga cccacgacat aatccgcgaa cagcgcccag ggcccggctt tgggattgtc
     2881 gccgtgcgca taagggccca ggacgcgatt gccgccggcc cagatcagga acggcatccc
     2941 gaggatcc
//
LOCUS       DATLKL010000835         1627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_111686, whole genome shotgun sequence.
ACCESSION   DATLKL010000835 DATLKL010000000
VERSION     DATLKL010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111686"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /gene="glp"
                     /locus_tag="VM713_11410"
     CDS             <1..1097
                     /gene="glp"
                     /locus_tag="VM713_11410"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HVH84910.1"
                     /translation="PVAAARAQPPFRAAAMDGYGVRAADLGPVPLAVLGEAAAGRAYA
                     GAPLRAGGAVRIFTGAAVPAGVELIVPQERARREGDSVWLETATQRRSNIREAGVDFA
                     AGEVLVHRGVRLNARHLALIAASGAGTVEAARRPHVAVLATGDELVQPGALAAADQIF
                     DSVSFGVAAAIGEWGGEALRLAIRPDAEAAIAVAAADALRRTDLLVVIGGASVGDYDV
                     VKRSLAGLGLEIAVERVAVRPGRPTWFGRAAGKPVLGLPGNPAAALVCAHLFLRPLLA
                     AMLGRGGEGAQESGAVALLEGELEASGANECYLRAIAVRGADTELRVRPCGNQDTSLL
                     SVFAAANALIRRPALAPAVGSGQPVEVLWL"
     gene            1094..>1627
                     /locus_tag="VM713_11415"
     CDS             1094..>1627
                     /locus_tag="VM713_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVH84911.1"
                     /translation="MSLGPSADARIPLGSSGLRVAPLGWGMWRLAEPAEASPRERVEA
                     ALEAGCTLFDTADVYGYRHGSGFGTAELLLGKVLREAPRLRDSLVLATKAGITPPVPY
                     DSSAEHLIAACEASLERLGVEYIDLYQIHRPDLLAHPGEVAQALERLRRAGKIRAAGV
                     SNYSAAQFEALAGHLPFA"
BASE COUNT          189 a          580 c          616 g          242 t
ORIGIN      
        1 atcccgtagc cgccgcacgc gcgcagccgc cgttccgtgc ggcagccatg gacggatacg
       61 gggtgcgcgc cgcggatctc ggccctgtgc ctctcgccgt gctcggcgag gcggcggccg
      121 gccgcgcgta tgccggtgcg ccgctgcgcg ccgggggcgc ggtccgcatc ttcactggcg
      181 cggcggtgcc tgcgggcgtg gagctcatcg tgccgcagga gcgtgccagg cgcgagggcg
      241 attcggtgtg gctggagacc gccactcagc ggcgcagcaa catccgtgaa gcaggcgtcg
      301 acttcgccgc cggtgaggtg ctggtgcacc ggggcgtgcg gctcaacgcg cgccacctcg
      361 ctctcatcgc ggcatccggc gccggtaccg tggaggcggc gcggcggccg cacgtggccg
      421 tgctcgccac gggcgatgag ctggtgcagc ccggtgccct tgccgcggcc gaccagatct
      481 tcgactcggt gagtttcggc gtggccgccg caatcgggga gtggggcggg gaggcgctgc
      541 gtctcgcgat ccgccccgac gcggaggccg ccatcgccgt tgcggccgcc gacgcgctgc
      601 gccggacgga tctgctcgtc gtcatcggcg gagcgtcggt cggcgactac gacgtcgtga
      661 agcggtccct tgccggcctc ggcctcgaaa tcgcggtcga gcgcgtcgcg gtccgcccgg
      721 gtaggccgac ctggttcggg cgagccgcgg gcaagccggt gttgggactg cccggcaatc
      781 cggcagcggc cctggtgtgc gcgcacctgt tcctgcggcc cttgctcgcc gccatgctcg
      841 gtcgcggggg ggagggcgcc caggaaagcg gcgcggtggc gcttctcgag ggcgagctcg
      901 aggcgagcgg cgcgaacgaa tgctacttgc gcgccatcgc tgtgcgcggc gcggacaccg
      961 agctgcgcgt gcgcccttgc ggcaaccagg acacctcgct cctctcggtg ttcgcggctg
     1021 cgaacgcact catccgccga ccggcgctcg cgcccgcggt cggaagcggc cagccggtcg
     1081 aggtgctgtg gctgtgagcc tcgggccctc ggcggacgcg cgcattcccc tcggcagcag
     1141 cggcctccga gtggcaccgc tcggctgggg tatgtggcgt ctcgcagagc cggcagaggc
     1201 gagcccgcgc gagcgggtgg aggcggccct cgaggccggc tgtacgctgt tcgacaccgc
     1261 ggacgtctac ggctatcggc acggcagcgg cttcgggact gccgaattgc tcctcggcaa
     1321 ggtgttgcgc gaggcgccgc ggctgcgcga ttctctggtg ctcgccacca aggccggcat
     1381 caccccgccg gtgccctacg actcctcggc cgaacacctc atcgcagcct gcgaagcctc
     1441 tctcgagcgc ctcggcgtcg agtacattga tctctatcag attcaccgcc ccgacctgct
     1501 ggcgcacccc ggcgaagtgg ctcaggccct ggagcggctg cggcgcgcgg gcaagatccg
     1561 cgcggccggc gtatcgaact acagcgcggc gcagttcgag gccctggccg gacatctgcc
     1621 gtttgcg
//
LOCUS       DATLKL010000836         1884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_10010, whole genome shotgun sequence.
ACCESSION   DATLKL010000836 DATLKL010000000
VERSION     DATLKL010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10010"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VM713_11420"
     CDS             complement(<1..1488)
                     /locus_tag="VM713_11420"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HVH84912.1"
                     /translation="MPRSSSAARWLYRLLLLGAALLAGAPAPAQIVSAGPIRLVSVID
                     VAEQEDRVDLTMVFNCSMRFVSSLPPSEGKEVRIQLAPLPDCAVSPLALIATETPPVL
                     GGAGIVRSARMEGIAPGQATLTISFVKSEQFVIAQSADPHGLRLRILNHKRQKPQVLV
                     GGQPAELALYAINLDSEPKAFAQADLDLAHSRLKAPIYVSEVTVSDQKWYRLRAGPIA
                     DRKDADRLLDLALHEYPRAWLAIGDDSATAVAGAAPGQAEALPAVEKIGSDPPLQPEE
                     LKRLMAEARVAMDKHDYKTAITVLTRLQRQPEFPDRASAQELLGLARERSGELAHAKA
                     EYEEYLRRYPNGEAAERVALRLRTLRNAEARARTGREAPGEKRGWEMSGDWSQLFRYD
                     GQRITTGAPPPNTSSTVPLGPLTQNENAVFNDIDLLARNRGNTFDLIGRLSAGYDKSF
                     QTSATALANPTRVSIASFEVLDRPLGLLARLGRQVSVEDGLLGTFD"
     gene            complement(1475..>1884)
                     /locus_tag="VM713_11425"
     CDS             complement(1475..>1884)
                     /locus_tag="VM713_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84913.1"
                     /translation="NNLAATGLPPNHFVTHLDCSSCHSYPDWSVISFHHVLTAAYPGD
                     HRVALTCNKCHTTNTEQVAYAAPAYAGTCAGCHAKNYLAAKHPKIAGGRVLYTVGELA
                     NCSGACHVYSDATLTTIVKSQPGPKHRVMDAAF"
BASE COUNT          269 a          617 c          682 g          316 t
ORIGIN      
        1 gtcgaaggtg ccgagcaggc cgtcctcgac gctcacctgg cggcccagac gggccaggag
       61 cccgagcgga cggtcgagca cctcgaacga ggcgatgctc acccgggtgg ggttggcgag
      121 cgccgtggcg ctcgtctgga agctcttgtc gtagccggcg ctcaggcgcc ctatcaggtc
      181 gaacgtgttc ccgcggttgc gcgcgagcag gtcgatgtcg ttgaagaccg cattctcatt
      241 ctgagtcagc gggccaagcg gcacggtgga cgaggtgttg ggaggcggcg ccccggtggt
      301 gatccgctgg ccgtcgtagc gaaacagctg tgaccagtcg ccgctcatct cccagccgcg
      361 cttctcgccc ggcgcctcgc ggccggtgcg cgcccgggcc tcggcgttgc gcagagtgcg
      421 caggcgcagc gccacgcgct cggccgcctc gccgttggga tagcggcgca gatactcctc
      481 gtactccgcc ttggcgtgcg cgagctcgcc ggagcgctcg cgcgcgaggc ccagcagctc
      541 ctgggcgctc gcccggtcgg ggaactccgg ctggcgttgc agcctggtca gcacggtgat
      601 ggccgtcttg tagtcgtgct tgtccatggc gacgcgcgcc tcagccatca gccgcttcag
      661 ctcctcgggc tgcagcggcg gatcactgcc gattttctcg accgcgggca aagcctccgc
      721 ctggccgggg gcggcgcctg cgacggccgt cgcagagtcg tcgccgatcg cgagccaggc
      781 gcgcgggtac tcgtgcagtg ccagatccag caaccggtcg gcgtccttgc ggtccgcaat
      841 cggaccggcg cgcagccggt accacttctg gtcgctgaca gtgacctcgg acacgtagat
      901 cggcgctttt agccgcgagt gggcgagatc gaggtccgcc tgcgcaaaag ccttgggctc
      961 ggagtcgagg ttgatggcgt acagcgcgag ctccgcgggc tgtccgccga ccagcacctg
     1021 gggcttctgg cgcttgtgat tgaggatccg cagccgcagg ccgtgcggat cggcgctctg
     1081 cgcaatcacg aactgctcgc tcttgacgaa cgagatggtg agggtcgcct gccccggcgc
     1141 gatcccttcc atgcgcgcac tgcgcacgat acccgcgccg ccgagaaccg gcggcgtctc
     1201 ggtggcgatc agggcgaggg gactcaccgc gcagtccggc agcggcgcca gctggatgcg
     1261 gacttccttg ccttcgctcg gcggcaggct gctgacgaag cgcatcgagc agttgaacac
     1321 catggtcagg tcgaccctgt cctcctgctc ggcgacgtcg atgacgctca cgaggcggat
     1381 cggtcccgcg gacacgatct gagcgggcgc gggcgccccc gcgagcagag ccgcgccgag
     1441 cagcagtagc cggtaaagcc agcgcgcagc gctgctagaa cgcggcatcc atgactcggt
     1501 gtttcggtcc gggctggctc ttcacgatag tggtgagggt cgcgtcgctg tagacatggc
     1561 aggcgcctga gcagttggcg agctcaccga cggtgtaaag cacacggcca ccggcgatct
     1621 tcggatgctt ggcggcgagg tagttcttgg catgacaccc cgcgcacgtg ccggcgtagg
     1681 ccggagccgc gtaggccacc tgctcggtgt tggtggtgtg gcacttgttg cacgtgagcg
     1741 ccacgcggtg atcgcccgga taggcggcgg tcaatacgtg gtggaagctg atgacgctcc
     1801 agtccgggta gctgtggcag ctcgagcagt cgaggtgcgt cacgaagtga ttcggcggca
     1861 gcccggtcgc cgccaggttg ttgt
//
LOCUS       DATLKL010000837         1525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_89244, whole genome shotgun sequence.
ACCESSION   DATLKL010000837 DATLKL010000000
VERSION     DATLKL010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89244"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..1443)
                     /locus_tag="VM713_11430"
     CDS             complement(349..1443)
                     /locus_tag="VM713_11430"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II asparaginase"
                     /protein_id="HVH84914.1"
                     /translation="MQNRLIHLRPIGAIGSLLAILLLWTPGYAQDAGKLPSVMILATG
                     GTIAGTGSTSTTTVGYTAAKVGVEALIEALPELKKVANVKGEQVFQIASENMNNDYWL
                     KLAKRVNTLLAQNDVDGIVITHGTDTIEETAYFLNLVVKSRKPVVIVGAMRPSTAISA
                     DGPINLYNAVILAGSKEAVGKGVLVCLNDQINGGRDVTKTNTSTADTFRTPELGFLGY
                     MQDNRPHFYRESTRKKTADTEFDIMSLDSLPRVDIVYGYANEDRVAIDAFVASGAKGI
                     VHAGVGDGSLARPAVEPALIDARKKGVVIVRSSRVGNGIVARNGEAKDDELDFVVSDT
                     LNPQKALILLMLALTKTNDAKEIQRMFYTY"
BASE COUNT          232 a          493 c          507 g          293 t
ORIGIN      
        1 tcgccgccga ggcgctgccg cgcggcgggc ggattgcgct cgtgctcgcg ccaggcgggg
       61 caggcatgga ggtcgcgata tccgccgagg gtgagcaagc cggcctcggc ggggaaatca
      121 tcgacgccat taagggtgag ctggccgccg acgcgctcac ggcgcgcacg gcccatgcct
      181 acttcaccgg ccgcgtctca gcccggctcg agcttcccgt gtctattgcc caatcgcctg
      241 ggcgcgtgac ttttggcgtg agccttcccg gggggtcatg aaggcacgcg ccggccgcgg
      301 acgcgaagga gccagccacg ctgcgcgcag gccgcaagcc aatgcggatc agtacgtgta
      361 gaacatccgc tggatttcct tggcgtcgtt ggtcttggtc agcgccagca tgagcaggat
      421 gagcgccttc tgcggattga gcgtgtcgga caccacgaaa tccaattcgt cgtctttcgc
      481 ctcgccgttg cgggcgacga tcccgttgcc gacccggctg ctgcgcacga tcaccacgcc
      541 cttcttgcgc gcatcgatca atgccggctc gacggccggg cgcgccaggc tgccgtcgcc
      601 cacgcccgca tggacaatgc ccttagcacc ggacgcgacg aatgcgtcga tcgcgacccg
      661 gtcctcgttg gcgtagccgt agacgatgtc gacccgcggc aaggagtcga ggctcatgat
      721 gtcgaactcg gtatcggcgg tcttcttgcg ggtggactcc ctatagaagt gcggccggtt
      781 gtcctgcatg tagccgagaa agccgagctc gggcgtgcgg aaggtgtcgg ccgtcgaggt
      841 gttcgttttc gtgacgtcgc ggccgccgtt gatctgatcg ttgaggcaga ccaggacccc
      901 cttgcccacc gcctccttgc tgccggcgag gatgaccgcg ttgtagaggt tgatcggccc
      961 gtccgcgctg atcgccgtgg aggggcgcat cgccccgacg atcacgaccg gcttcctgct
     1021 cttgaccacc aggttcagga agtaggcggt ctcctcgatg gtatccgtgc cgtgggtgat
     1081 cacgatcccg tccacatcat tctgggcgag cagcgtgttg acccgcttgg ccagcttgag
     1141 ccagtagtcg ttgttcatgt tctcgctagc gatctggaac acctgctcgc ccttgacgtt
     1201 ggccaccttc ttgagctccg gcagcgcctc gataagcgcc tccacgccga ccttggcggc
     1261 ggtatagccg acagtggtgg tgctggtgct gccggtcccg gcgatcgtgc cgccggtggc
     1321 caggatcatc acactgggga gcttgcccgc atcctgggca tagcctggcg tccagaggag
     1381 caggatcgcg agaagcgatc cgatcgcacc aatcggacga agatggataa gacggttctg
     1441 catgattcct ccctgccgaa cggcttagcg ccttgggcgc gccagcgtcg gactggcttt
     1501 ttgttgctgg ccgcgcgctc gcgaa
//
LOCUS       DATLKL010000838         1847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_213123, whole genome shotgun sequence.
ACCESSION   DATLKL010000838 DATLKL010000000
VERSION     DATLKL010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213123"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VM713_11435"
     CDS             <1..485
                     /locus_tag="VM713_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVH84915.1"
                     /translation="IGIILLTARKDEPDRIKGLSLGADDYLPKPFSPQELVLRVAAVL
                     RRLAAPAVAAGGLLAAGPIALDKTAHRVTVEGDEVDLTATEFKLLRTLIEREGRVQSR
                     AQLLETVWRAQPDIQTRTVDMHVQRLRQKLGKAGDCVETVRGAGYRFRRPEPRARRRP
                     "
     gene            482..>1847
                     /locus_tag="VM713_11440"
     CDS             482..>1847
                     /locus_tag="VM713_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVH84916.1"
                     /translation="MTLVSRLFITSSLLVVVAVGGLIVVADRTSRRSFEDDVARGLER
                     EARLVATLLPPDSARWPDYARALGASIGHRVTLIDGKGWVRGDTEFDRAALAKLENHG
                     TRPEVLHALAEGVGRDERLSASTSVRQLYVAVRGGPPGLTVVRLSAPLTLVDAQVGVV
                     QRGVALGGLAAILGAAALAWLLARDVSKPLGELSSAARAIAAGQPPTFPDSRVPEVAE
                     HVLALRAMHEELAHRFAELRREREDTATLIETMADGVLASDARGRIITLNAAARRLLG
                     YSPSDSLPALAELFHDKPARDLVDAILAGREAQAIDLALGQRALFLAGRALPNGGALL
                     VIRDVTELRRLETVRRDFVANLSHELKTPLTSIAGYAETLAAEAAPDTQAEQFARTIL
                     NNARRMQRLVDNLLDLSRIESGRWQPAPQAVDITHVGRETWASFATRAQERKIEFAIE
                     VAPPG"
BASE COUNT          257 a          699 c          626 g          265 t
ORIGIN      
        1 agatcggcat cattctcctc acggcgcgta aggatgagcc cgaccggatc aaaggactgt
       61 cgctcggggc cgacgactac ctcccgaagc cgttctcgcc acaggagctc gtgctccgcg
      121 tagcggccgt gctccggcgc ctcgcggcgc ccgccgtggc cgccggcggc ctgctggccg
      181 ccggcccgat cgcgctcgac aagaccgcgc accgcgtcac cgtcgaaggc gatgaggtgg
      241 acctcacggc aacggagttc aagttgctgc gcacgctcat cgagcgggaa ggacgcgtgc
      301 agagccgggc ccagctgctc gaaacggtgt ggcgtgccca gcccgacatt caaacgcgca
      361 ccgtcgacat gcacgtgcaa cggctgcggc agaagctcgg caaggccggc gattgcgtcg
      421 agacggtccg cggggccggc tatcgcttcc gccgccctga gccccgggcc cgccggcgtc
      481 cgtgacgttg gtcagccggc tgtttatcac ttcgtcgctg ctcgtcgtcg tcgcggtcgg
      541 gggactgatc gtggtcgccg accgaacgtc ccggcgcagc tttgaggacg acgtcgcgcg
      601 cggcctcgag cgcgaggcgc gcctcgtggc gaccttgctg ccccccgact ccgcccgctg
      661 gcccgattac gctcgggcgc tcggtgcgag catcgggcac cgggtgacct tgatcgacgg
      721 caagggctgg gtccgcggtg acaccgagtt cgatcgtgcc gcgctcgcca agctcgagaa
      781 ccacggcacc cgccccgaag tcctgcacgc gctagccgag ggcgtgggcc gggacgagcg
      841 cctgagcgcg tcgaccagcg tgcgccagct gtatgtcgcc gtgcgggggg gccccccggg
      901 cctgaccgtc gtccgcctgt ccgcgccact cacgctggtc gacgcgcagg tcggcgtcgt
      961 gcagcggggc gtcgcgctcg ggggtctcgc cgcgatcctc ggcgcggccg cgctcgcgtg
     1021 gctgctcgcc cgcgacgtct cgaagccgct cggcgagctc agctcggccg cccgagcgat
     1081 cgccgccggc cagcctccca cgtttcccga ctcccgagtc cctgaagtgg ccgagcacgt
     1141 gctcgctctc cgcgccatgc acgaggagct cgcgcaccgc ttcgccgagc tccgccgcga
     1201 gcgcgaagac acggcgaccc tgatcgagac catggccgac ggggtgcttg cttcagacgc
     1261 gcgcggtcgg atcatcacgc tcaacgccgc cgcccggcgc ctcttgggct acagtccgtc
     1321 agactcgttg ccggcgctcg ccgagctgtt ccatgataag cccgcccgcg atttggtcga
     1381 cgcgattctc gcagggcgcg aggcccaagc gatcgacctg gcgctcggac agcgggcgct
     1441 cttcctcgcg ggccgcgccc tgccgaatgg aggcgcgctg ctcgtgatcc gcgacgtcac
     1501 cgagctccgc cgcctcgaga cggtacgtcg cgacttcgtc gcgaacctct cgcacgagct
     1561 gaagacgccg ctcacgtcca tcgcaggata cgccgagacc ttggccgccg aggccgcgcc
     1621 cgacacccag gccgagcagt tcgcccgcac catcctcaat aacgcccgcc ggatgcagcg
     1681 gctcgtcgac aacctgctcg acctgtcgcg gatcgaatcg ggccgctggc agccggcgcc
     1741 ccaggccgtc gacatcaccc acgtcggccg cgagacctgg gcgtcgttcg ccacgcgagc
     1801 gcaggagcga aagatcgagt tcgccatcga ggtcgcgccc cccggcg
//
LOCUS       DATLKL010000839         3208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_134485, whole genome shotgun sequence.
ACCESSION   DATLKL010000839 DATLKL010000000
VERSION     DATLKL010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134485"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VM713_11445"
     CDS             complement(<1..189)
                     /locus_tag="VM713_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVH84917.1"
                     /translation="MQSRRLGPRGPAVSALGLGCMGMSEFYGAHSDVESSATLDRALE
                     LGVNFFDTADDYGPYTNEE"
     gene            complement(314..1684)
                     /locus_tag="VM713_11450"
     CDS             complement(314..1684)
                     /locus_tag="VM713_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVH84918.1"
                     /translation="MQRVLTEGSIPVTLFRFALPILYANVLQSLNGSVNSVWVGRFLG
                     EAALTATSNANTVMFLLIGAAFGVALAATILVGQCIGANDIPETKRVVGTSATFFTAI
                     SVAMAIAGLVLCRPLLIAMSTPADSLDLAVAYMRVIFLALPFLYLYAYVGAMLRGAGD
                     SKTPFYFMLLSVGLDIALNPLFIFGLGPIPRLGIAGSALATFVAQAVSLSALIRHLYR
                     RRHLLVLHKDELALLRVDWSVVATLVKKGLPMSAQMLVLSLSGVLMITLVNRFGVDTT
                     AAFGAALQLWNYIQMPAFAVAMAMSAMTAQNVGAAKWDRVMQIARVGVFYNVVLCGGI
                     ALAMELLDTRLFALFLPAGSAALSAATHLNRVVTPSFVFFGVAVALFGVVRATGAVMA
                     PLVILTITLLVVRFPLAELYLDRYQVDAVWWSFPISSALSALLAVLYFKYGGWRSAHM
                     LPAGAA"
     gene            complement(1685..>3208)
                     /gene="ubiB"
                     /locus_tag="VM713_11455"
     CDS             complement(1685..>3208)
                     /gene="ubiB"
                     /locus_tag="VM713_11455"
                     /inference="COORDINATES: protein motif:HMM:NF003404.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HVH84919.1"
                     /translation="YLSPDVWLERRRAGARGERLRLALEELGPIFVKFGQAVSTRRDL
                     LPADIADELARLQDRVPPFDGAVARQLIEASYGRPVTELFASFDETPLAAASIAQVHP
                     ARLPNGAEVVVKVLRPGMRAVIERDLEVLHALADLAQRYWHEGRRLRPGELVDEYHKT
                     VLDELDLMREAANASQLKRNFAGSDLLYVPEVCWDYCRTDVMVMERIHGVPISDLAAL
                     KAAGTNIARLAENGVRIFFTQVFRHNFFHADMHPGNIFVIIDDPQRPRYAAVDFGIVG
                     TLDPRDQHYLAENFLAVFDRDYRRVAQLHLESGWVPAGTRIDEMESAVRTVLEPIFDR
                     PLKDISFGRILLRLFEISRRFDMQIQPQLLLLQKTLLNVEGLGRELYPELDIWSTASP
                     ILREWMRERTSLSALWRSVRSHAPEILEALRGLPALLAAQLRRGAQPLPSALPGDELA
                     QLREELLREGRRRDLVTVGAALLLGGLVWLAVSGAHTVAGWALIAAGAVAALAGFGR"
BASE COUNT          564 a         1090 c         1126 g          428 t
ORIGIN      
        1 ctcttcgttg gtgtacggtc cgtaatcgtc cgcagtgtcg aagaaattga cgccgagctc
       61 gagcgcccga tcgagcgtcg cgctcgactc cacgtccgag tgcgcgccgt agaactcgct
      121 catgcccatg cagccgaggc cgagcgccga gacggcgggg ccgcgggggc cgagtctgcg
      181 cgactgcatg ggcgtactcc ttgaccgggc ggggcgccca ttgtccgccc gcgcgcccgc
      241 gccacgaata ctcacttcga tggagggccg gcctgcgcct cagggcggag ccgctccggg
      301 ccggtggcgg cggctacgcg gcacccgccg gcagcatgtg cgcgctgcgc caaccgccgt
      361 acttgaagta gagcaccgcg agcagtgccg agagcgcgga cgagatcggg aagctccacc
      421 acacggcgtc cacctgatag cggtccaagt agagctcggc gagcgggaag cggaccacga
      481 gcagcgtgat cgtgaggatg acgaggggtg ccatgacggc acccgtcgcg cgtaccacgc
      541 cgaagagcgc caccgccacg ccgaagaaca cgaacgatgg cgtgaccacg cggttgaggt
      601 gcgtggcggc gctgagcgcc gcagagccgg ccggcaggaa cagcgcgaac agccgcgtgt
      661 cgagcagctc catggcgagc gcaatccccc cgcacagcac gacgttgtag aagactccga
      721 cgcgcgcaat ctgcatgacc cggtcccact tggcggcgcc gacgttctgg gccgtcatgg
      781 ccgacattgc catggcgacc gcgaacgccg gcatctggat atagttccac agctgcagcg
      841 cggcgccgaa ggccgccgtg gtgtcgaccc cgaagcggtt tacgagggtg atcatgagca
      901 cacccgagag cgacagcacc agcatctgcg cgctcatcgg cagacccttc ttcaccagtg
      961 tcgcgaccac tgaccagtcg accctgagga gcgccagctc gtccttgtgc agcaccagca
     1021 ggtgccgccg ccggtagagg tggcggatga gcgcgctgag gctcacggcc tgggcgacga
     1081 aggtggcgag cgccgagccc gcgatcccca gccggggaat cggccccaag ccaaaaatga
     1141 acagcggatt gagcgcgatg tcgaggccga ccgacagcag catgaaatag aacggggtct
     1201 tcgagtcgcc ggcgccgcgc agcatcgcgc cgacgtaggc gtacaggtag agaaacggca
     1261 gcgccaggaa gatcacccgc atatacgcca cggcgaggtc gagcgagtcg gccggcgtgc
     1321 tcatggcgat cagcagcggg cggcacagca cgaggcccgc gatcgccatc gcgactgaga
     1381 tggcggtgaa gaaagtggcg ctggtgccga ccacccgctt agtctcgggg atgtcattgg
     1441 cgccgatgca ctggccgacc aggatcgtgg ccgcgagcgc cacgccgaag gccgcgccga
     1501 tcagcaggaa catcaccgtg ttggcattgg aggtcgcggt gagcgccgcc tcgccgagaa
     1561 agcgccccac ccagacggag ttcaccgagc cgttgaggga ctgcagcacg ttggcgtaca
     1621 ggatcggcag cgcgaacctg aagagtgtga cggggatcga cccctcggta aggacgcgct
     1681 gcactcagcg cccgaagccg gcgagtgccg cgacggcgcc cgcggcgatg agcgcccagc
     1741 cggccaccgt gtgggcgccc gagaccgcga gccacacgag cccaccgagt agcagcgctg
     1801 caccgaccgt cacgaggtcg cggcgccgcc cctccctcag gagctcctcg cgcagctgcg
     1861 cgagctcgtc gcccgggagc gcgctcggca gcggctgcgc gccgcggcgc agctgcgccg
     1921 cgagcagcgc cggcagtccg cgcagcgctt cgaggatctc gggcgcgtgg gagcggacgc
     1981 tgcgccacag ggcgctgagg ctcgtgcgct cgcgcatcca ctcgcgcagg atcgggctcg
     2041 cggtgctcca gatatcgagc tcggggtaga gctcccttcc caagccctcg acgttgagca
     2101 gcgtcttctg cagcaggagc agctgtggct ggatctgcat gtcgaaacgc cgcgaaatct
     2161 cgaacaggcg cagcaggatc cgcccgaagg agatgtcctt cagcggccgg tcgaagatcg
     2221 gctcgagcac ggtgcgcacc gccgactcca tctcgtcgat gcgcgtgccc gccgggaccc
     2281 agcccgactc gagatgcagc tgcgcaacgc ggcggtagtc gcgatcgaac accgcgagga
     2341 aattctccgc gaggtagtgc tgatcgcgcg gatcgagcgt gccgacgatg ccgaagtcga
     2401 ccgcggcgta acgcggccgc tgcggatcgt cgatgatgac gaagatgttg cccgggtgca
     2461 tgtcggcgtg gaagaaattg tggcggaaca cctgggtgaa gaagatccgc acgccgttct
     2521 cggcgaggcg cgcgatgttg gtgcccgccg ccttgagcgc cgccaagtcg ctgatcggca
     2581 ctccgtggat gcgctccatc accatgacgt cggtgcggca gtagtcccag cacacttcgg
     2641 ggacatacag caggtctgag ccggcgaagt tgcgcttcag ctgcgaggcg ttggcggcct
     2701 cgcgcatcag gtccagctca tcgagcaccg tcttgtggta ctcgtccacg agttcgcctg
     2761 gcctgaggcg ccgcccctcg tgccaatagc gctgcgcgag atccgcgagc gcgtgcagca
     2821 cctcgagatc gcgctcgatg accgcgcgca tcccagggcg cagcaccttg accaccacct
     2881 cggcgccgtt cggcagccgt gccgggtgca cctgggcgat cgaggcggcg gcgagcgggg
     2941 tctcgtcgaa agaggcgaac agctccgtga ccggccggcc gtagctcgcc tcgatgagct
     3001 gccgcgccac ggcgccgtcg aagggcggca cgcggtcctg caatctcgcg agctcgtcgg
     3061 cgatgtccgc cggcaggaga tcgcggcgcg tcgagaccgc ctgcccgaac ttcacgaaga
     3121 tcggcccgag ctcctcgagc gcgagccgca gccgctcgcc gcgcgcgcct gcccggcgcc
     3181 gctcgagcca gacgtcgggg gacaggta
//
LOCUS       DATLKL010000840         2462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_89306, whole genome shotgun sequence.
ACCESSION   DATLKL010000840 DATLKL010000000
VERSION     DATLKL010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89306"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VM713_11460"
     CDS             complement(<1..997)
                     /locus_tag="VM713_11460"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HVH84920.1"
                     /translation="MHRVLILGAGKIGALISGLLAESGSYKVHLADMDGAAAEAVARA
                     HGGLELTPFSLDASQPELLERHLATHPVDAVISGLPYYCNRAVAEVARRVRAHYFDLT
                     EDVEVTRAVRAIASGASQAFVPQCGLAPGFISIAAAELITHFEELRSVKLRVGALPQH
                     PNNVLKYSLTWSTEGLINEYGNPCQAISDGRMVEVAPLEGLEEIEIDGMRYEAFNTSG
                     GLGSLADTHGAHCETMNYKTIRYPGHCEQMRLLMNDLKLNHDRGTLKRILENAVPQTL
                     QDVVIVYAAVTGRQEGQLREENYVNKIYPQVIAGRLWSAIQVTTAAGITAVVDLVL"
     gene            1128..2420
                     /locus_tag="VM713_11465"
     CDS             1128..2420
                     /locus_tag="VM713_11465"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF013628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HVH84921.1"
                     /translation="MRSPETTSWHSASWQTRPAQQQPVYRDPKALERTVAELSRLPPI
                     VVSWEVEALRARLAAAQRGEAFLLQGGDCAETYADCESDTIAKKLKILLQMSLVLLHG
                     LKKPIIRVGRMAGQYAKPRSADTESRDGVTLPSYRGDLVNRPEFTAEARAADPMLLLR
                     GYERAALTLNFVRALVEGGFADLHHPEYWDLGFVHQAPLKDAYQRIVKSIGDALDFFE
                     GISGRQVHEATLVDFYSSHEGLHLHYEQAQTRFIPRQNRWYNLSTHMPWIGMRTAALD
                     GAHVEYFRGISNPIAVKVGSGMDAAWLQGLVTTLNPQNQPGRLTFIHRFGAKDVAAAL
                     PKAIEAVRQTGQTVLWVCDPMHGNTETTTGGLKTRRFENILRELDLAFRIHADLGSYL
                     GGVHIELTGEDVTECTGGARGLTDQDLARAYRYSPNSL"
BASE COUNT          408 a          797 c          820 g          437 t
ORIGIN      
        1 cgagcaccag gtccacgacc gcggtgatgc cggctgcggt cgtcacctga atggccgacc
       61 acaagcgtcc ggcgatgacc tgcgggtaga tcttgttgac gtagttctcc tcgcgcagct
      121 gcccctcctg cctgccggtc acggcggcgt agacgatcac cacgtcctgg agggtctgcg
      181 ggaccgcatt ctccaggatg cgcttcaacg tgccgcggtc gtgattgagc ttgaggtcgt
      241 tcatgagcag gcgcatctgc tcgcagtgtc cggggtagcg gatggtcttg tagttcatcg
      301 tctcgcagtg cgcgccatgg gtgtcggcaa gcgagccgag gccccctgag gtattgaagg
      361 cctcgtagcg catgccgtcg atctcgatct cctcaagccc ctcgagcggt gccacttcca
      421 ccatgcggcc gtcgctgatc gcctggcacg gattgccgta ctcgttgatg agtccctcgg
      481 tggaccaggt gagggagtac ttgaggacgt tgttggggtg ctggggcagg gcgccgacgc
      541 gcagtttcac cgaccgcagc tcctcgaagt gggtaatgag ctcggccgcg gcgatgctga
      601 tgaagcccgg agcgaggccg cactgcggca cgaacgcctg ggacgccccc gaggcaatgg
      661 cacgcacggc gcgcgtgacc tcgacgtcct cggtgaggtc gaaatagtga gcgcggacgc
      721 gccgtgccac ttcggccacc gcgcggttgc agtagtaggg gagacccgag atgacggcat
      781 cgacggggtg ggtcgccagg tgccgctcga ggagctcggg ctgactcgcg tcgagcgaaa
      841 aaggcgtgag ctccaggccg ccgtgggcac gcgccacggc ctcggccgcc gcgccgtcca
      901 tgtcggcgag gtggactttg taactgcctg attctgcgag taatccggat atcagggcgc
      961 cgatcttccc ggcgccaagg atcagtacgc ggtgcatagg gtcctctcga aaaggcgcgc
     1021 attctgcctt cggcgaggcc gcaacgccag ccaccgttcc gagcccttcc gcgggccctg
     1081 cgacgcccag tcatgagccc ccggaggcac gtgctaggat cgatttcatg cgctctcccg
     1141 agacgacttc ctggcacagc gcgagctggc agacccggcc cgctcagcag cagccggtct
     1201 atcgcgaccc caaggcgctc gagcgcaccg tggccgagct gtcgcggttg ccgccgatcg
     1261 tcgtgtcgtg ggaggtcgag gcgctgcggg cgcggctggc ggcggcgcag cgcggggagg
     1321 cgtttctgct gcagggcggt gactgcgccg agacgtacgc ggactgtgag tccgacacga
     1381 tcgcgaagaa gctgaagatc ctgctgcaga tgagcctggt gctgctgcac gggctgaaga
     1441 aaccgatcat ccgggtgggc cgcatggccg gccagtacgc caagccccgt tctgccgaca
     1501 ccgagagccg cgacggggtg acgctgccga gctaccgcgg cgatctcgtc aaccgtccgg
     1561 agttcaccgc cgaggcccgc gctgcagatc cgatgctgct gctgcgcggc tacgagcgtg
     1621 cggcgctgac tctcaatttc gtgcgtgcct tggtcgaagg gggctttgcg gacctgcatc
     1681 atccggagta ctgggacctg ggcttcgtgc atcaggcgcc actcaaggat gcctaccagc
     1741 ggatcgtgaa gtcgatcggc gatgcgctcg atttcttcga gggtatcagc gggcggcagg
     1801 tgcatgaggc gacgctggtg gatttctact cgagccatga ggggctgcat ctgcattatg
     1861 agcaggcgca gacgcgcttc atcccgcgcc agaaccgctg gtacaacctc tcgacccaca
     1921 tgccctggat cggtatgcgc accgccgctc tcgacggcgc ccacgtcgaa tacttccgcg
     1981 gcatctccaa tccgatcgcc gtcaaggtgg gctccggaat ggatgccgcc tggctgcagg
     2041 gactcgtgac tacgctcaac ccgcagaacc aacccgggcg gctcacgttc atccaccgct
     2101 tcggggccaa ggacgtcgcc gcggcgctcc ccaaagccat cgaggcggtg cgccagaccg
     2161 ggcagacggt gctgtgggta tgcgacccca tgcacgggaa caccgagacg accaccgggg
     2221 gcctcaagac gcgccggttc gagaacatcc tgagggagct tgacctcgcc ttccgcattc
     2281 acgcggacct cggctcctat ctcggcggcg tgcacatcga gctcaccggc gaggacgtca
     2341 ccgagtgcac cgggggcgcg cgcggactca ccgaccagga cctggcgcga gcctatcgct
     2401 attcaccaaa ttctctctaa gttgttaatc tatatctcta ttttcatttt ttgcttcttt
     2461 ca
//
LOCUS       DATLKL010000841         1682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_224167, whole genome shotgun sequence.
ACCESSION   DATLKL010000841 DATLKL010000000
VERSION     DATLKL010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224167"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..>1682
                     /locus_tag="VM713_11470"
     CDS             139..>1682
                     /locus_tag="VM713_11470"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HVH84922.1"
                     /translation="MDRRRLANAVVPLLALAVLSGGGRAQAPVPPASPAAAAPPAEGP
                     PPAHPLTRPRAPVAQPVQKRPWADARFAQVEHDYVIYFLGRFPVVATYLGGATLDPQL
                     AGVDGRLRDHSPEALRAEDAQLAEFRARFAHLAPAGLSPRRRIDRSVALAEIAFLEHE
                     HGVRRQQQKSLDSYVDEPARGVDWQIQSMTTGGGAAYGTDAEWQAVIARVRAVPGYLA
                     TAERQIAAGVAAHNTPDGRVLVDFGLRSAAADADYFASTLPQLAAGAITSAQRAALLH
                     DLTAAGEAAAAAYRHLAAMIASVFFEDTGGGEPALRAEFRADRFACGETEYDWALRNN
                     LRLSSTAAELFAQSSPAVEATRAEMIALARQIAASHRWPRTGDGPASVRAVFEQLSRD
                     APQSDAEMLEGYRRAGARLTAYGRATGLFDVPADYRLDVLATPPPLRASVASGGYYPA
                     PPFKSTGTGRFYVTPTGDNAAALRVQHDYASMADTAAHEGFPGHDWHFKVMTRYREEI
                     SPLRWL"
BASE COUNT          230 a          623 c          595 g          234 t
ORIGIN      
        1 cgaacagtcc cttacgagtg cccagccaca gcgtgctcat gcgaacgagc atcctcgccc
       61 ggcgacgagg ctgtcaacaa gggtatgctt cgccgcttac ctcatcgcgc cccgcgcgcc
      121 ctgggagtgc aaggacccgt ggatcgccgc cgcctcgcaa acgcggtcgt gccgcttctg
      181 gcgctggccg tgctgagcgg cggcgggcgc gcgcaggcgc cggtgccgcc ggcttcgccg
      241 gcggccgcag ctccgcccgc cgagggcccg ccgccggccc acccgctgac tcgaccgcgc
      301 gcgcctgtgg cgcagcccgt gcagaagcgc ccctgggctg atgcgcgctt cgcgcaggtc
      361 gagcacgact acgtgatcta cttcctcggg cggttcccgg tagtcgcaac ctacctcggg
      421 ggcgccacgc tcgatccgca gctcgccggg gtcgacggcc ggctgcggga tcattcgccc
      481 gaggcgctcc gcgctgagga cgcgcagctc gcggagtttc gggcgcgctt cgcgcacctc
      541 gcgcccgcgg gcctgtcgcc gcggcggcgc atcgaccgct cggtcgcgct cgccgagatc
      601 gccttcctgg agcatgagca cggcgtgcgc cgccagcagc agaaatccct cgactcctac
      661 gtggatgagc cggcacgcgg cgtcgactgg cagatccaga gcatgacgac cgggggcggt
      721 gcggcttacg gcacggacgc cgagtggcag gcggtcatcg cacgcgtgcg ggccgtgccc
      781 ggctatctcg cgaccgccga gcggcagatc gctgcgggcg tcgccgcgca caacacaccc
      841 gacgggcgcg tacttgtcga cttcggactg cgcagcgccg ccgccgatgc cgactacttc
      901 gccagcacgt tgccgcagct ggcggccggc gccatcacct cggcgcagcg cgcggcgctg
      961 ttgcacgacc tcacggccgc gggggaggcc gccgcagcgg cttaccggca tctcgcggcg
     1021 atgatcgcga gcgtcttctt cgaggacacg ggcggcggcg agccggcgct cagggccgaa
     1081 ttccgcgctg atcgcttcgc ctgcggtgag accgagtacg actgggcgct gcgcaacaat
     1141 ctgcgcctca gcagcacggc ggccgagctg ttcgcacaat cgtcgccggc ggtggaagcg
     1201 acgcgtgccg agatgatcgc gctggcgcgg cagattgcgg cgagccatcg gtggccgcgt
     1261 acgggcgacg ggccggcgag cgtgcgggcg gtgttcgagc agttgagccg cgacgcgccg
     1321 cagagcgacg ccgaaatgct cgaaggctat cgccgcgcgg gagcgcggct caccgcctac
     1381 ggccgagcga cgggactgtt cgacgtgccc gccgattacc gcctcgacgt cctcgcgacg
     1441 ccgccgcccc tgcgtgcctc ggtcgccagc ggcggctact acccggcgcc accgttcaag
     1501 agcaccggca cgggccgctt ttacgttacg cccaccggcg acaacgcggc ggcgctgcgc
     1561 gttcagcacg actacgcctc gatggctgac accgccgcac acgagggctt ccccgggcac
     1621 gactggcatt tcaaggtcat gacgcgctat cgcgaggaga tctcgccgct gcgctggttg
     1681 tt
//
LOCUS       DATLKL010000842         2100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_21478, whole genome shotgun sequence.
ACCESSION   DATLKL010000842 DATLKL010000000
VERSION     DATLKL010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21478"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VM713_11475"
     CDS             <1..629
                     /locus_tag="VM713_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVH84923.1"
                     /translation="RGTARPVIAGDLVLCGFDNGKVLAVNSGDGSVQWEAAIAPPHGR
                     TELERLDDIDTAVRVSGQDVYTVGFQGKIAMLAIDTGQVWWSHDASSYRSLALDDDTL
                     YLASADGEIAALKTRTGAELWRQKALLHRGLTAPAAVDAGNALVVADFQGYLHFLDRA
                     TGALAARVSTGRVRVSTTPVVVGNVVLVVNDRGHISAYRVTPLITARK"
     gene            672..2045
                     /gene="der"
                     /locus_tag="VM713_11480"
     CDS             672..2045
                     /gene="der"
                     /locus_tag="VM713_11480"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HVH84924.1"
                     /translation="MLPVIALVGRPNVGKSTLFNVLTGTRDALVADVPGLTRDRQYGF
                     GRVGPVPYVVIDTGGLIENATGIEAQMRAQTERAVAEADRLVFVTDARAGLSPEDQFV
                     ARELRRSGKPVTLALNKAEGLDADVAGADFHALGLGEPHAISASHGQGCESLMEQVLA
                     GFEPQPAAPEADGAIRIAIIGRPNVGKSTLVNRLLGEERVIASEQPGTTRDSILVPFE
                     RDGRQFLLIDTAGVRRRARVADAVERASVAKTLQAIAEAHVVILVLDAHDTVAEQDAS
                     VLGLALERGRALIIAVNKWDGIPEEQRAEIQRQLALKLDFVPFAPLFFISARHGTGVG
                     ELMKSTVRAYEGAMRTLPTKELTRTLEQALSAHQPPLVHGRRIKLRYAHQGGRNPPRI
                     VIHGNQTASVPEAYTRYLASLFRKTYDLFATPVAIEYRTDANPYDRERRGGRAPQRKR
                     RPPARHR"
BASE COUNT          322 a          755 c          730 g          293 t
ORIGIN      
        1 tgcgcggcac ggcgcgcccg gtgatcgccg gcgacctcgt tttatgcggc ttcgacaacg
       61 gcaaggtgct cgccgtcaac tcgggcgatg gctcggttca gtgggaggcg gcgatcgcgc
      121 ccccgcacgg gcgcacggag ctcgagcgcc tggacgacat cgacaccgcg gttcgcgtct
      181 ccgggcagga cgtctacacg gtcggcttcc agggcaagat cgccatgctg gcgatcgata
      241 ccgggcaggt ttggtggtcg cacgacgcct cgagctatcg cagcctcgcg ctcgatgacg
      301 acaccttgta cctggcgagc gccgacggcg agatcgcggc gctcaagacg cgcaccggcg
      361 cggagctgtg gcggcagaag gcgctgctgc accgcggact caccgcacca gccgccgtgg
      421 acgcgggcaa cgcgctggtg gtggcggact ttcagggcta cctccatttc ctcgacaggg
      481 ccaccggcgc cctcgccgcg cgggtctcaa ccggcagggt gcgcgtcagc actacgcccg
      541 ttgtggtcgg caacgtggtg ctggtggtga acgaccgcgg ccacatcagc gcctatcgcg
      601 tcacgccgct catcactgcg cgcaagtagc cgcgtgcacc ggccgccggc tccgcgccgc
      661 accgcgcacc catgctcccg gtcatcgcgc tcgtcggccg cccgaatgtc ggcaagtcga
      721 cgctcttcaa cgtcctgacg ggcacccgcg acgcgctggt cgcggacgtg cccgggctca
      781 cgcgcgaccg gcaatacggc ttcgggcgcg tcgggcccgt tccatatgtg gtgatcgata
      841 ccggcgggct catcgagaac gcgaccggga tcgaggcgca gatgcgcgcg cagaccgagc
      901 gcgccgtcgc cgaggctgac cgtctggtct tcgtcaccga tgcccgcgcc gggctctcac
      961 ccgaggatca attcgtcgca cgcgagctgc gccgctcggg caagcccgtg accctcgcgc
     1021 tcaacaaggc ggagggactc gacgccgacg tcgccggcgc cgactttcac gctctgggct
     1081 taggcgagcc gcacgccatc tcggcgagcc atggccaggg ctgtgagagc ctgatggagc
     1141 aggtgctggc gggcttcgag ccgcagcccg ccgcgccgga agcggacggg gcgatccgca
     1201 tcgccatcat cgggcgcccc aacgtcggca agtcgaccct ggtcaaccgg ctgctcggcg
     1261 aggagcgggt catcgcgagc gagcagccgg ggactacgcg cgacagcatc ctcgtgccct
     1321 tcgagcgcga cggccggcag ttcctgctca tcgataccgc gggggtgcgg cgccgggcgc
     1381 gcgtcgcgga tgcggtggag cgcgcgagcg tcgccaagac gctgcaggcg atcgcggagg
     1441 cgcacgtcgt catcctggtg ctcgacgccc acgacacggt cgccgagcag gacgcgagcg
     1501 tgctcgggct cgcactcgag cgcgggcgcg cgctcatcat cgcggtcaac aagtgggacg
     1561 gaattcccga ggagcagcgc gccgagatcc agcgccagct cgccctcaag ctcgacttcg
     1621 tgccgtttgc gccactcttt ttcatctcgg cgcggcatgg cacgggagtg ggcgagctca
     1681 tgaagtcgac ggtgcgggcc tacgaagggg cgatgcgcac gctgccgacg aaggagctga
     1741 cgcgcacact cgagcaggcg ctcagcgcgc atcagccgcc gctggtgcac ggccggcgca
     1801 tcaagctgcg ctacgcacac cagggcggac gcaatccgcc gcgcatcgtc atccacggca
     1861 atcagaccgc ctcggtcccg gaggcgtaca cgcgctatct cgcgagtctc tttcgcaaga
     1921 cctacgacct gttcgcgacc ccggtcgcca tcgagtaccg caccgacgca aatccctacg
     1981 accgggagcg ccgcggcgga cgcgctccgc agcgcaagcg ccgtccgccg gcccggcacc
     2041 gctgaggggg cgagcgcggg aggggtcgca cccctcccga cccgggtttc atcgcatcat
//
LOCUS       DATLKL010000843         2136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_32931, whole genome shotgun sequence.
ACCESSION   DATLKL010000843 DATLKL010000000
VERSION     DATLKL010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32931"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VM713_11485"
     CDS             <1..1283
                     /locus_tag="VM713_11485"
                     /inference="COORDINATES: protein motif:HMM:NF036501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84925.1"
                     /translation="MMKIPDGAEWDCFAGLEPPQVALERFLADVPFARRYPRRYDAPA
                     VEVPTMLPAPREPRMSTQEGFGRILNEIARGDSPLAARVVTASPDVTVSTNLGAWVNR
                     RGVFMRERQEDVFREEQVVSAQRWVQSPEGQHVELGIAEHNLFILLAALGLAGPLFGV
                     RLLPIGTLYDTFIKRGLDALNYALYQDARFMLVATPSGITLAPEGGAHQSIVTPLIGL
                     GLPNLEVYEPAYVDGLAEIMRWGFGHMQEPDGGSLYLRLSTRALAQPERSLEQALSQA
                     IIAGGYWMVPPAQGAELGIVYSGAVAPEAGEAYRQILEDVPGTGLLAVTSVSRLHADW
                     RTRREASHIARLLEPLAPDAALGSVADAHPATLAWLGSVGRHRAYPLGVDHFGQSGDI
                     PDLYRVYGLDADAIIEAAAAACLARVTGGSAAAT"
     gene            1374..1460
                     /locus_tag="VM713_11490"
     tRNA            1374..1460
                     /locus_tag="VM713_11490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1408..1410,aa:Leu,seq:tag)
     gene            1559..>2136
                     /gene="tig"
                     /locus_tag="VM713_11495"
     CDS             1559..>2136
                     /gene="tig"
                     /locus_tag="VM713_11495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HVH84926.1"
                     /translation="MLSGFTMQVTQTAVDGLKHEFKVVLSAKDIEAKIDHRLSELGRE
                     VRVPGFRPGKVPLAVLKKRYGDAVVGEVVQRAVSDSSAQAIMDQGLRPALQPKIEITA
                     FAKGNDLEYKLAVEVLPQVEPIDLTKIKLERLVAEVTDKEVEESLERLAAQTKRTEPA
                     LAGHKAAKGDVLVIDYKGSIGGEAFPGGEAEG"
BASE COUNT          349 a          690 c          736 g          361 t
ORIGIN      
        1 cgatgatgaa gatcccggac ggcgccgagt gggactgctt tgcggggctg gagccgccac
       61 aagtggcgct cgaacggttc ctcgccgacg tgccgttcgc ccggcgctat ccccgccgct
      121 acgatgcccc cgcggtcgaa gtgccgacca tgcttccagc accccgcgag ccccgaatgt
      181 cgacccagga ggggttcggc cggatcctga acgagatcgc ccgcggcgat agcccgctcg
      241 ccgcgcgcgt cgtcaccgct tcgccggacg tcaccgtatc gaccaatctc ggcgcctggg
      301 tcaatcggcg tggcgtgttc atgcgcgaac gccaggagga tgtcttccgc gaggaacaag
      361 tcgtttccgc ccagcgctgg gtgcagtctc ccgagggtca gcacgttgag ctcggaatcg
      421 ccgagcacaa cctatttatt ctgctcgccg ccctcggcct cgcggggccg ctgttcggcg
      481 tccgtctgtt gccgatcgga accctttacg acaccttcat aaagcgcggg ctcgatgccc
      541 tcaattacgc gctctatcag gacgcccgct tcatgctggt ggcgaccccc tcggggatca
      601 cgctcgcgcc tgagggtggc gcacaccagt cgatcgtgac gccgctgata gggcttggcc
      661 tgcccaactt ggaggtctat gagccagcct atgtggacgg gctcgccgag atcatgcgct
      721 ggggcttcgg ccacatgcag gagccggatg gcgggtcgct ctatctccgg ctgtcaacta
      781 gggcgctcgc gcaaccggag cggtccctcg agcaggcgct cagccaagcg atcatcgccg
      841 gcggatactg gatggttccg cccgcccagg gcgccgagct cggcatcgtc tatagcggcg
      901 ccgttgcgcc ggaagccggc gaggcctatc ggcagatcct cgaggatgtg cccggtaccg
      961 gactgctcgc cgtgacgtcg gtgagccgac tccacgccga ctggcggacg cggcgcgagg
     1021 cgagccacat cgcccggctt ctagagccct tggcgcccga tgcggcgcta ggctcggtag
     1081 cagacgcgca tcccgcaacg cttgcctggc tgggctcggt gggtcggcat cgcgcgtacc
     1141 ctctcggcgt cgatcatttc ggtcagtcgg gcgacattcc cgatctctac cgcgtctacg
     1201 ggctcgacgc cgatgccatc atcgaggccg cggctgcggc atgtcttgct cgggtcaccg
     1261 gcggctcggc ggctgccact tgaaccccgt ggggcaaggg cttaatagaa ccgtccgggc
     1321 gagttcggcg ttgacggagg ctttccaggc aggcagccgg gtgggttcga gccgcgggcg
     1381 tggcggaact ggtagacgcg ccaggtttag gacctggtgg ccgtgaggcc gtgggggttc
     1441 aagtccctcc gcccgcacca ggtgctgcag gaggcgcggg gcttggcttg gcaggtcggc
     1501 gccgagtttg acatttcggc gcggcctttg acattaaggc ctggccacct ccggccgaat
     1561 gttgtcggga ttcacgatgc aagtaacgca aacggcggtc gacggcctga agcatgagtt
     1621 caaggtcgtg ctctccgcca aggacatcga agcgaagatc gatcatcgac tgagcgagct
     1681 cggtcgcgag gtgcgcgtgc cggggttcag gcccggcaag gtgcctctcg ccgtcctcaa
     1741 gaagcgttat ggcgacgccg tggtgggcga ggtggtccag cgcgcagtgt ccgacagctc
     1801 ggcacaggcc atcatggatc aggggctgcg accggcgctg cagcccaaaa tcgagatcac
     1861 cgccttcgcc aagggcaacg acctcgaata caagctggcc gtcgaggttc tgccccaggt
     1921 agagcccata gacttgacca agatcaagct cgagcggctg gtggccgagg tcacggacaa
     1981 ggaggtcgag gagtccctgg agcggcttgc cgcccaaacc aagaggaccg aaccagccct
     2041 ggccgggcac aaggccgcga aaggcgatgt gctggtgatc gattacaagg ggtcgatcgg
     2101 gggcgaggcg ttccccggag gcgaggccga ggggca
//
LOCUS       DATLKL010000844         1659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_145721, whole genome shotgun sequence.
ACCESSION   DATLKL010000844 DATLKL010000000
VERSION     DATLKL010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145721"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1416
                     /gene="ccrA"
                     /locus_tag="VM713_11500"
     CDS             133..1416
                     /gene="ccrA"
                     /locus_tag="VM713_11500"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HVH84927.1"
                     /translation="MGEAAKVVELSPGGSRKDLYEIGEIPPLGHVPSRMYAWAIRRER
                     EGEPERAMRLEVVPTPSIQADEVLVMVMAAGVNYNGVWAALGKPVSVFDVHKAEYHVA
                     GSDAAGLVWAVGAKVKRWKVGDEVVVHCNQDDGDDEECNGGDPMFSPSQRIWGYETPD
                     GSFAQFARVQSRQLMPRPKHLTWEESGCYTLTLATAYRMLFGHRPHILRPGHNVLVWG
                     SAGGLGSMAVQLIATAGANAIGVISDDAKRDFVMQLGARGAINRSRFACWGRMPDVAE
                     TERYGAYLAEVRKFGRAIWEITGKGNDVDFVFEHPGEATFPVSCFVVKRGGMVVFCAG
                     TTGYNITFDARFVWMRQKRIQGSHFANLLQASQANRLVIERRIDPCMSEVFPWTDIPK
                     AHTKMMRNEHKPGNMAVLVQARRPGLKTLEDALHA"
     gene            1448..>1659
                     /locus_tag="VM713_11505"
     CDS             1448..>1659
                     /locus_tag="VM713_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84928.1"
                     /translation="MSHAALRARRGDSTAPLLPDLLVSCASGLDAAQRYLGRVRLAVA
                     AKVTRAGALDADRLEREQFAAHGYAW"
BASE COUNT          278 a          556 c          579 g          246 t
ORIGIN      
        1 taaaacaacg cagtgtgcat tgcaaaataa tggattccga agtatgaatg taagctcttg
       61 ttgcaccgcg gggcaacccg ggcggtgccg ccacccccgg ggcgatcccc tcgtgatgcg
      121 aggaggcgtg cgatgggcga agcggcaaag gtggtcgagc tcagtcccgg cggatcccgg
      181 aaggacctgt acgagatcgg cgaaatcccg ccgctcggcc acgtgccgag ccggatgtac
      241 gcctgggcga tccggcgcga gcgcgagggc gagccggagc gcgccatgcg gctcgaggtc
      301 gtccccacgc ccagcattca ggcggacgag gtgctggtga tggtgatggc ggccggcgtc
      361 aattacaacg gcgtctgggc cgctctcgga aagcccgtct ccgtgttcga cgtgcacaag
      421 gcggaatacc acgtcgccgg gtcggatgcc gccggcctgg tgtgggcggt cggcgccaag
      481 gtgaagcgtt ggaaggtggg cgacgaggtg gtggtccact gcaaccagga cgacggcgac
      541 gacgaggagt gcaacggcgg cgacccgatg ttctcgccgt cccagcgcat ctggggctac
      601 gagacgcccg acggctcctt cgcccagttc gcgcgcgtgc agtcgcgcca gctcatgccg
      661 cggcccaagc acctcacctg ggaggagagc ggctgctaca ccctcaccct ggcgaccgcc
      721 taccgcatgc tgttcggcca ccgcccgcat atccttcgcc ccggacacaa cgtgctggtc
      781 tggggctcgg ccggcgggct cggctcgatg gcggtgcagc tgatcgccac ggccggcgcc
      841 aacgcgatcg gcgtcatctc tgacgacgcc aagcgcgatt tcgtcatgca gctcggcgcg
      901 cgcggcgcca tcaaccgcag ccgctttgcc tgctggggcc gcatgccgga tgtggccgag
      961 accgagcgat acggcgccta cctcgcggag gtacgcaagt tcggccgcgc catctgggag
     1021 atcaccggca agggcaacga cgtcgacttc gtgttcgagc atcccggcga ggcgacgttc
     1081 ccggtgagct gcttcgtggt caagcgcggc ggcatggtgg tgttctgcgc cgggaccaca
     1141 ggctacaaca tcaccttcga tgcccgcttc gtgtggatgc gtcagaagcg catccagggc
     1201 agccacttcg ccaatctgct ccaggcgagc caggccaacc gcctagtgat cgagcggcgc
     1261 atcgatccct gcatgagcga ggtcttcccc tggaccgaca tccccaaggc ccacaccaaa
     1321 atgatgcgca acgagcacaa gccgggcaat atggcggtcc tggtgcaggc gaggcgcccg
     1381 ggcttgaaaa cgctcgagga cgcactccac gcctgacgct gcgccccatt ggcggagccc
     1441 gaagacaatg agccacgccg cgctgcgcgc gcgcaggggg gactcgacgg cgccgctgtt
     1501 gccggacctc ctcgtgtcgt gcgcaagcgg gctcgacgcg gcgcagcgct atctgggccg
     1561 tgtgcggctc gcggtcgcgg ccaaggtgac gcgcgcgggc gcgctcgacg ccgatcgcct
     1621 cgagcgcgag cagttcgcgg cccacggcta cgcgtggag
//
LOCUS       DATLKL010000845         1667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_10143, whole genome shotgun sequence.
ACCESSION   DATLKL010000845 DATLKL010000000
VERSION     DATLKL010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10143"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VM713_11510"
     CDS             <1..629
                     /locus_tag="VM713_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVH84929.1"
                     /translation="GHGASGANRADWSIANYAADVATVARQIPNARLVLVGHSMGADV
                     SLAAVPRIGTRVIGVIAVDALRSVGMPPLSAGEIAAKVAPFRADFIGATRALVSDQLF
                     EQGADPLLVQKVAYDMSLRSPAVALPTLEKLLAFDFATVLPAVRVPVYAINSDLAPTD
                     AERIRKSLPGFTLEVLPHTGHFLMLEAPAQFNPLLMKDIDALEARAAH"
     gene            complement(650..1501)
                     /locus_tag="VM713_11515"
     CDS             complement(650..1501)
                     /locus_tag="VM713_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HVH84930.1"
                     /translation="MDQRLADILQLLELEPLEVNLFRGESRDIGAPQVFGGQVLGQAL
                     TAAAATVEGRVVHSLHAYFLRRGDINAPIVYQVDRSLDGHSFTNRRVVAIQHGEQIFN
                     MTTSFQVTEGGFDHQIDMPQVPPPEKLPDPAPAPELLEKLPEKVRRFFSRPRPFEFRL
                     VQPLDYLRHRSAPPVRQVWVRAVGKLGDDEMLHRRLLAYVSDFFLLDTATLPHGTSFL
                     KPSLIMATIDHAMWFHRPLRVDDWLLYAMESPSASGARGFARANIFARDGRLVASAAQ
                     EGLIRQR"
BASE COUNT          260 a          569 c          568 g          270 t
ORIGIN      
        1 caggtcacgg cgcctcgggc gccaatcgcg ccgactggtc gatcgccaac tacgccgcgg
       61 acgtcgcgac cgtggcgcgg cagatcccga acgcgcgcct cgtgctggtt gggcactcga
      121 tgggggccga cgtatcgctc gcggcagtcc cgcgcatcgg cacgcgcgtc atcggcgtca
      181 tcgcggtcga tgcgctgcgc tcggtcggca tgccgccctt gagcgccggc gagatcgcgg
      241 ccaaggtcgc gccgttccgc gccgacttca tcggcgcgac gcgcgcactg gtctccgacc
      301 agctcttcga gcagggcgcc gatccgctgc tggtgcagaa ggtggcctac gacatgtcgt
      361 tgcgctcgcc ggcggtcgcg ctgccgactc tcgagaagct gctggcgttc gattttgcga
      421 ccgtgctgcc cgccgtgcgc gtgccggtgt acgccatcaa ctccgatctc gccccgaccg
      481 atgccgagcg catccgcaag tcactgcctg gtttcacgct cgaggtgctg ccgcacaccg
      541 ggcacttcct catgctggaa gcgcccgcac agtttaatcc gctgctgatg aaggatatcg
      601 acgcgctcga ggcgcgcgcc gcccactgaa gcggcggcga gccgcgcacc tatctctggc
      661 gtatgaggcc ttcctgggcg gcgctcgcca ccaggcggcc gtcgcgggcg aagatgttcg
      721 cgcgcgcgaa gccgcgcgct ccggaggcac tcggactctc catcgcgtac agcagccagt
      781 catcgacccg cagcggccgg tggaaccaca tggcgtgatc gatcgtcgcc atgatgagcg
      841 agggcttgag gaacgaggtg ccgtgcggga gcgtcgcggt gtcgagcagg aagaagtccg
      901 agacatacgc aagcagccgc cggtgcagca tctcgtcgtc gccgagcttg ccgacggcac
      961 gcacccagac ctggcgcacg ggcggcgccg acctgtgtct caggtagtcg agcggttgca
     1021 ccaggcggaa ctcaaaggga cgcggccggg agaagaagcg ccgcaccttc tcgggcagtt
     1081 tctccagcag ctccggcgcc ggggccgggt ccggcagctt ctccggcggc ggcacctgcg
     1141 gcatgtcgat ctgatggtcg aagccgccct cggtcacctg gaacgaggtg gtcatgttga
     1201 agatctgctc gccgtgctgg atcgcgacga cgcggcggtt ggtgaagctg tgcccgtcga
     1261 ggctgcggtc gacctgatag acgatcggcg cattgatgtc gccgcgcctg agaaaatagg
     1321 catgcagcga atgcaccacc ctcccctcga cggtcgcggc cgcggcggtc agcgcctgtc
     1381 cgagcacctg cccgccgaac acctgcggcg cgccgatgtc gcggctctcg ccgcggaaca
     1441 gattcacctc cagcggctcg agctcgagca gctggaggat atccgcgaga cgctggtcca
     1501 tcgccacccc cccaaaggct cgcttcagtt gaacttgaac ttgccgtgca tcagggcgct
     1561 gtgacccggg aaggtgcaca ggtagctgta gtccccgccc ttgctgagga tcgaggcgga
     1621 gaaagtgacg ctggtggtct gaccgccgcc gatcaccttg gtatgcg
//
LOCUS       DATLKL010000846         8480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_202143, whole genome shotgun sequence.
ACCESSION   DATLKL010000846 DATLKL010000000
VERSION     DATLKL010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202143"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3585)
                     /locus_tag="VM713_11520"
     CDS             complement(<1..3585)
                     /locus_tag="VM713_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HVH84931.1"
                     /translation="MRVRPADAAAGTDAPAAVQPGAVHARTLLRVTLITVAGVALLAT
                     VVLFTWALARARVPQQRATLENLVHAETGLDVRFSELRLRWGWYGPEALLQSVELREP
                     GETRALLTAPQLVLGVDLWRMLRSGELAISRITLLDPSIDATQRAPARTPAPGGAASA
                     SPTRLLARWRGTRIDVKGGTLSLGLAGEPLEAGIRRIQLRRAGAEWRAEALLTLPEAL
                     GTTAEVSLRLEGDVLRPAELAGRLTLSSSGLQLAGWRSLLRSAPLAQYLPGAGSADVT
                     AQLDLAPGGVLNAQGAVAAAAVKWPAVGAGAGPALDELRADWHLARNRAGWHLAVEPL
                     ELGPGLPRAALSIDAAADGGWVRGRLQRAPVAVLAGLGRGVLPPLRLSGVELAGMVRE
                     ASFDWNAARPPEARLLTSAALDDLSITPSGHALTLAGLTARLGSTGASLEAALEAHSA
                     RATFAADPALVLGSIGVRTRLALADNGHAWRVGVRELEIRRGDARLAVSGALAGEDSG
                     AHPRLDVRASVSGVPVQLVRAALGAPLLAALGTAAGELTAGRIEHAELVARGPLDEPL
                     PWSGARQEFTGTLALSGASLAALREWPDLYGINARIDWRGARVRVRADGAVAGGFRLS
                     AVRGEWDARAASLSHLAGRLTGDAADALAWLRNHPGLESYAPGIDGIALSGGTALDFE
                     LKRPDGPMREPPLPHFSTHLSALLDGAQLRPVAGLPVIAGLRGTLVFADGHLQRSTLT
                     GQWLGGPVALSVAERRERGVAAAAISAKGVLDVREAVAAATGLPAREAPLHGNADWSA
                     ELKLLPGTGGERPSWRARADSSLAGVTSELPEPLAKAPGSMLPLHIELLGTEDAGELR
                     VALADRLRGIAAVSRRDELWRIDRGAVRLSGRAPTLPRAPVMQIEGTASRLDLPGYAL
                     LWRRLARDPAWPALLVTVNANELLAADRSFPEVRVSAQAGQGAGELTLDSRDLDALVH
                     WPAVVDAAHPVSAYLERLDLAQLAGGSTAHAALLAALASDTQLSIGDLLWQGRSLGGL
                     DATVALRSGALEVHDLHVSGADDDARGELQCRRGSCRASFSLTSHDAGATLARIGFRS
                     EVTAARALAGGELEWRTAGDAILADASGRLHVELDDGHIRRVARADAPGRALGLLAVP
                     GLIAGMGLTQLPFTRLSADFAVADGEAVTTNLHLDGDTEILLRGRIGLI"
     gene            complement(3595..5079)
                     /gene="rng"
                     /locus_tag="VM713_11525"
     CDS             complement(3595..5079)
                     /gene="rng"
                     /locus_tag="VM713_11525"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HVH84932.1"
                     /translation="MTTEIVVNVAPRETRAAILENGVVQEIHVERTSRRGLVSNLYKG
                     RVSRVLPGMQAAFVEIGLDRTAFLHAADIARAGADDTVASTLADASPAPEDIRRLLNP
                     GDDILVQVIKDPIGSKGARLTTFIALPSRYLVYLPRGQAIGVSARIEDEGERARLKGV
                     LAGLVGAATPGGYIVRTAAQGASPESLSEDMAYLARLWEHVRARAAAAAPGSVVHEDL
                     PLSLRVLRDELARGVSRVLVDSAREQAVMLEFAAAFVPGVAPVIELYSGPRPIFDLNG
                     VEEEVNRALDRKVPLKSGGHLVFDQTESMTTIDVNTGAYVGHRNLEETIFRTNLEAAV
                     TIARQLRLRNLGGIIIIDFIDMLDEGHRRAVLAALERALAGDRAQTHIVSLSPLGLVE
                     MTRKRTRESLEHLLCEPCRICEGRGFVRTPETVCNEIFREIVRQSRQFASRELLILAH
                     PDVVERLLDEESATLAELEAQVGRPIRLQVEALYGVDQYDVVLV"
     gene            complement(5076..5672)
                     /locus_tag="VM713_11530"
     CDS             complement(5076..5672)
                     /locus_tag="VM713_11530"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HVH84933.1"
                     /translation="MPPAVPQLCLASVSPRRRELLEQIGVAHVVSGADIDEMAAVGES
                     PADYVVRMACAKARAVRARGTQLPVLAADTTVVIDGLILGKPRDRAEGVAMLGRLSGR
                     THQVLTAVALATAAGVAFRLSASEVRLRAITPEECAAYWETGEPRDKAGGYAIQGRGA
                     LFIEHLSGSYSGVMGLPLFETGQLLAAAGVACLAGGAP"
     gene            complement(5678..6148)
                     /gene="rlmH"
                     /locus_tag="VM713_11535"
     CDS             complement(5678..6148)
                     /gene="rlmH"
                     /locus_tag="VM713_11535"
                     /inference="COORDINATES: protein motif:HMM:NF000986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HVH84934.1"
                     /translation="MRVRLIAVGTRPPGWVRDACEDYIMRLGSRLPVSITEIAAGPRT
                     GSAPSAKAIAREGERLLAALGRGDYVIALDERGTELSTRELAAWLKTRMQEGEDLAFV
                     IGGPDGLAPEVLARSQLRWSLSRLTLPHALVRVLLAEQLYRAHSLLAGHPYHRD"
     gene            complement(6158..6574)
                     /gene="rsfS"
                     /locus_tag="VM713_11540"
     CDS             complement(6158..6574)
                     /gene="rsfS"
                     /locus_tag="VM713_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HVH84935.1"
                     /translation="MTTRTRLRPAAERVSSLKDAVTTALDDMKAVNVRVLDVRGLTDI
                     ADTMVIASGNSDRHVRSIAERVVEKAKAIGRRPIGTEGERDGDWVLVDLQDVIVHVML
                     PRVREFYGLERLWEGGEELAAPALAHAAARRRQAST"
     gene            complement(6607..7257)
                     /gene="nadD"
                     /locus_tag="VM713_11545"
     CDS             complement(6607..7257)
                     /gene="nadD"
                     /locus_tag="VM713_11545"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000839.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HVH84936.1"
                     /translation="MQQPIGLFGGTFDPIHCGHLRTAFELWQELKLAEVRFLPTGSPP
                     HRDHLYASAELRLKMVEVAIADQPGFVVDDREVRRSGVSYSVDTLTELRREYPQRSLC
                     LLLGMDAFLGLPSWHRWRELLELAHIVVAQRPGWKAPSKGPLGEVMVDHGTGAIRELH
                     ERPAGCLYVHAVTQLEISSTELRAALVAGRDPRYLVPDAVRQIILETRCYAAQQGP"
     gene            complement(7297..8265)
                     /gene="holA"
                     /locus_tag="VM713_11550"
     CDS             complement(7297..8265)
                     /gene="holA"
                     /locus_tag="VM713_11550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HVH84937.1"
                     /translation="MKLTSDSLANHLRERLLPAYLISGDEPLLAGEAADAVRAAARAA
                     GFSEREVHVAERAGDWDEVRGAARSLSLFGARRVFEIRMGARPGAAGNGALRALLEAH
                     DPDTLFLILTPRLDREAQGADWVRAVEAHGAWVPIWPVDAARFVGWLRGRARQLKLAA
                     SDAALELLAERTEGNLLAAHQELNKLALLAGDAPVTPEMVLASAADSARFDVFQLSEA
                     VLSGAAARALRVLAGLRAEGTEPTLVLWALTKALHDAWGALGGTGAPRGWQRQGAALE
                     AAARRAPHMPFTDLTARAARTDRVIKGRLAGNAWDELMLLTRALFA"
BASE COUNT         1245 a         3119 c         3018 g         1098 t
ORIGIN      
        1 gatcaggccg atgcgccccc gcagcagaat ctcggtgtcc ccgtccaggt ggaggttggt
       61 ggtaaccgcc tcgccgtcag ccaccgcaaa gtccgcgctg aggcgcgtga agggcagctg
      121 cgtcaggccc attcccgcga tgagtcccgg caccgccagg agccccaggg cgcggccggg
      181 cgcgtcggcg cgggcgaccc ggcggatgtg gccgtcgtcc agctccacgt ggagccggcc
      241 gctggcatcg gcgagaatcg cgtcgcccgc cgtgcgccat tcgagctcgc cgcccgccag
      301 ggcacgcgcc gcggtcacct cactgcggaa cccgatccgg gcgagcgtcg cgccggcatc
      361 gtggctcgtg aggctgaagc ttgcgcggca gctgccgcgg cggcattgca actcaccgcg
      421 tgcgtcatcg tccgcgccgc tgacatggag gtcgtggacc tcgagcgcgc cggagcgcag
      481 cgccacagtc gcatcgaggc cgccgaggga gcggccctgc cagagaaggt caccgatgga
      541 aagctgcgtg tcggacgcga gcgcagccaa cagggcggca tgagcggtgc tgccgcccgc
      601 gagctgcgcc agatcgagcc gctcgagata cgcgctcacg gggtgtgcgg cgtcgaccac
      661 tgcgggccag tgcaccaggg catcaaggtc ccgggagtcg agcgtgagct cccccgcgcc
      721 ctgccccgcc tgcgcggaga cgcgcacctc aggaaagctg cggtctgccg cgagcagctc
      781 gttcgcgttg accgtaacga gcagcgccgg ccaggcggga tcgcgggcga gccgacgcca
      841 cagcagggcg tagccgggta gatcgaggcg gctcgccgtg ccctcgatct gcatcacggg
      901 agcgcgcggc agggtgggcg cgcgccccga aagccggacc gcgccgcgat cgatccgcca
      961 cagctcgtcg cgccggctga cggccgcgat tccccgcagc ctgtcggcga gggcgacgcg
     1021 cagctcgcct gcgtcctcgg tcccgaggag ctcgatatgc agcggcaaca tggatcctgg
     1081 cgccttggcg aggggctcgg gcagctcgct cgtgacgccc gcgagactgc tgtcggcgcg
     1141 ggcacgccag ctcgggcgct caccgcccgt gcccggaagg agcttgagct ccgcgctcca
     1201 gtcggcgttg ccgtgcagcg gggcttcgcg ggcagggagc ccggtcgccg cggccaccgc
     1261 ttcgcgcaca tcgagcactc ccttggccga gatcgccgcc gccgcgaccc cgcgctcacg
     1321 gcgctccgcg acgctcagtg cgaccgggcc gccgagccac tgcccggtga gcgtcgagcg
     1381 ctgcaggtgg ccgtcggcaa agacgagcgt gccacgcagg cccgcgatca ccggcagccc
     1441 ggctaccggg cgcagctgcg ccccgtcgag gagggccgag agatgcgtac tgaagtgcgg
     1501 caacggcggc tcgcgcatcg ggccgtccgg gcgcttgagc tcgaagtcga gggccgtgcc
     1561 cccgctcagg gcgatgccgt cgatccccgg ggcgtaggac tcgagcccgg ggtgattgcg
     1621 cagccacgcg agcgcatccg ccgcatcgcc ggtgagcctg ccggcgaggt gcgagagact
     1681 cgccgcgcgc gcatcccact ccccgcgcac ggcgctcaag cggaagccac ctgcgacggc
     1741 gccgtccgca cgcacccgca cgcgcgctcc gcgccagtcg atgcgcgcat tgatgccgta
     1801 cagatcgggc cactcccgca gcgccgcgag gctcgcaccg ctgagggcga gggtccccgt
     1861 gaactcctga cgcgcgccgc tccagggcag cggctcatcg agcggaccgc gtgccacgag
     1921 ctccgcgtgc tcgatgcgcc cggcggtcag ctccccggcg gccgtgccaa gggcggcgag
     1981 cagcggagct ccgagggccg cacgcaccag ctgtaccggg acgccgctca cgctggcgcg
     2041 cacgtcgagg cgcggatggg cgcccgagtc ctcgcccgcg agcgcgccgc tcacggcgag
     2101 tcgcgcgtcc ccgcggcgaa tctcgagctc gcgcacgccg actcgccagg catgcccgtt
     2161 gtcggcgagc gcgagacgcg tgcgcacccc gatcgagccg agcacgaggg cgggatcagc
     2221 cgcgaaggtc gcccgcgcgc tgtgcgcttc gagcgccgcc tcgaggctcg cgccggtact
     2281 gcctaaacgc gccgtgagtc ccgcaagcgt gagcgcgtgg ccggaaggag tgatgctcag
     2341 gtcgtcgagc gcggcgctgg tgaggagccg cgcctccggc ggccgcgcgg cgttccaatc
     2401 gaagctcgcc tcgcgcacca tgcctgccag ctcgaccccc gagagacgca gcggcggcag
     2461 cacgccgcgc ccgagaccag cgagcaccgc gaccggcgcg cgttgcagcc gtccgcgcac
     2521 ccagccgcca tcggcggcgg cgtcgatgct gagagcggcg cggggcaaac ccggcccgag
     2581 ctcgagcggc tccaccgcga gatgccagcc ggcgcgatta cgcgccaggt gccagtcggc
     2641 gcgcagctca tcgagtgccg gtcccgcgcc ggctcccacc gcgggccatt taacggccgc
     2701 ggccgccacc gcgccctgtg cgttaagcac gccacccggt gcgagatcga gctgtgccgt
     2761 gacgtcggcg ctgcccgcac ccggcaggta ctgcgcgagt ggcgcggatc tgagcagcga
     2821 gcgccagccc gcgagctgca ggccgctgct gctcagggtg agtcggccgg cgagctccgc
     2881 gggccgcaac acgtcgccct cgaggcggag cgagacttca gccgtggtgc cgagcgcctc
     2941 cgggagcgtg aggagcgcct cggcgcgcca ttcggctccg gcgcggcgca gttggatccg
     3001 ccggatgccg gcttcgagcg gctcgcccgc gagacccaag ctcagcgtcc cgcccttgac
     3061 gtcgatgcgc gtgccgcgcc agcgcgcgag cagccgggtc gggctcgcgg acgccgctcc
     3121 ccccggcgcg ggcgtgcgcg cgggcgctcg ctgggtcgca tcgatgctgg gatcgagcag
     3181 cgtgatgcgg ctgatggcga gctccccgct gcgcagcatc cgccacaggt cgacgccgag
     3241 gacgagctgc ggggcggtga ggagcgcgcg cgtttcgcca ggctcgcgca gctcgacgct
     3301 ctggaggagg gcctcggggc cgtaccagcc ccagcggagc cgcagctcgc tgaagcgcac
     3361 gtcgagcccg gtctcggcat gcaccagatt ctcgagcgtc gcgcgctgct gcggcacgcg
     3421 cgcgcgggcg agcgcccacg tgaagagcac gacggtggcg agcagcgcaa cgccggcgac
     3481 tgtgatcagc gtcacgcgca ggagcgtgcg cgcgtgcacc gcccccggct gcaccgcagc
     3541 cggcgcgtcc gtcccggcag cggcatccgc gggcctcaca cgcatcacac gttcctagac
     3601 gagcacgacg tcgtactggt cgacgccgta gagcgcctcg acctgcaggc ggatcggtcg
     3661 cccgacctgc gcctcgagct ccgcgagcgt ggcggactcc tcgtcgagga gccgctccac
     3721 gacgtctgga tgagccagta tcagcagctc gcggctggca aactgtcggc tctgtcgcac
     3781 tatttcccta aagatctcgt tgcacaccgt ctccggggtc cgcacgaaac cgcgcccctc
     3841 acaaatccga cacggctcac acaacagatg ttccaggctc tcgcgggtgc gcttgcgggt
     3901 catctccacg agcccgagcg gcgagaggct cacgatgtga gtctgggcac gatcgcccgc
     3961 cagcgcccgc tccagcgcgg cgagcaccgc gcgccggtgg ccctcgtcaa gcatgtcgat
     4021 gaagtcgatg atgatgatgc cgccgaggtt gcgcaggcgc agttgccgcg cgatggtcac
     4081 cgccgcctcg aggttggtgc ggaagatggt ctcctcgaga ttgcggtgtc cgacataggc
     4141 gccggtgttc acatcgatgg tcgtcatcga ctcggtctga tcgaacacca ggtggccgcc
     4201 ggacttgagc ggcaccttgc gatcgagcgc gcggttcacc tcctcctcga cgccgttcag
     4261 gtcgaagatc ggccgcggtc ccgagtacag ctcgatcacc ggagccactc cgggcacgaa
     4321 cgcggcggcg aactcgagca tcacggcctg ctcgcgcgcg gaatcgacca gcacccggct
     4381 cacgccgcgc gcgagctcat cccgcaacac ccgtaagctg agcggcaggt cctcgtgcac
     4441 aaccgagccc ggcgcggcgg cggcggcgcg cgcgcgcacg tgctcccaca ggcgcgccag
     4501 ataagccatg tcttcgctca ggctctcagg gctcgctccc tgggccgccg tgcgcacgat
     4561 gtagccgccg ggggttgccg cgcccaccag gcctgcgagc acccctttca ggcgggcccg
     4621 ctccccctca tcctcgatcc gcgccgagac gccgatcgcc tgtccgcgcg gcagatacac
     4681 gaggtagcgc gagggcagcg cgatgaaggt cgtgagacgc gcgcccttgc tgccgatcgg
     4741 gtccttgatc acctgcacca gaatgtcatc gccgggattg aggaggcggc ggatgtcctc
     4801 cggcgcgggc gaggcatccg ccagcgtgct cgccaccgta tcgtcggcgc cggcgcgcgc
     4861 gatgtcggca gcgtgcagaa acgcggtgcg gtcgaggccg atctcgacga aggcggcctg
     4921 catccccggc agcacccgcg acacacgccc cttgtagagg ttgctcacca ggccgcgccg
     4981 gctggtgcgc tcgacgtgga tctcctgcac gacgccgttc tcgaggattg cagcgcgggt
     5041 ctcgcgcggc gccacgttga ccacgatctc ggtggtcaag gcgcaccccc cgccaggcac
     5101 gccacgccgg ccgcggcgag cagctgcccg gtctcgaaca gcggcagccc catcaccccg
     5161 gaataactgc cgctcaggtg ctcgatgaaa agcgcgcccc tcccctggat ggcgtagccg
     5221 ccggccttgt cgcgcggctc gcccgtctcc cagtacgcgg cgcattcctc gggcgtgatg
     5281 gcgcgcagcc gcacctcgct cgcgctcagg cggaaagcga cgccggcggc ggtcgcgagc
     5341 gccaccgcag tcagcacctg gtgggtgcgc cccgagagcc gcccgagcat ggcgacaccc
     5401 tcggcgcggt cgcgcggctt gccgagaatg agcccgtcga tcaccacggt ggtgtccgcg
     5461 gccagtaccg ggagctgcgt gccgcgcgcg cgcaccgcac gcgccttggc gcacgccatg
     5521 cgcaccacgt agtccgcggg agactcgcca acagctgcca tctcgtcgat gtcggcgccg
     5581 ctcaccacgt gcgcgacgcc gatctgctcg aggagctccc ggcggcgcgg cgacaccgag
     5641 gcgaggcaca gctgcggcac ggcgggcggc acggccttca gtcccggtgg taggggtgac
     5701 ccgcgagcag gctatgggcc cggtagagct gctcggcgag cagcacgcga accagggcgt
     5761 gcgggagcgt gaggcgcgac agcgaccagc gcagctggct gcgcgcgagc acttcgggcg
     5821 cgagcccgtc gggaccgcca atcacgaatg ccagatcctc gccttcctgc atgcgggtct
     5881 tgagccatgc ggcgagctcg cgggtcgaga gctcggtgcc gcgctcatcg agcgcgatca
     5941 cgtaatcgcc gcggccgagc gccgccagca gccgttcgcc ctcgcgcgcg atggccttcg
     6001 cggacggcgc actcccggtg cgcgggcctg ccgcgatctc ggtaatcgac accggcagcc
     6061 gcgagcctaa ccgcattatg taatcttcgc acgcgtcgcg cacccagccg ggagggcgcg
     6121 tgcccacggc gatcagccgc acgcgcatgg ggacgaatca ggtcgaggct tgccggcggc
     6181 gggcagcggc gtgggccagg gcgggcgcgg cgagctcttc gccgccttcc cacagccgct
     6241 cgaggccgta gaactcgcga actcgcggca gcatcacgtg aacgatgacg tcctgcaggt
     6301 cgacgagcac ccagtccccg tcgcgctccc cctcggtgcc gatcgggcgc cgcccgatcg
     6361 ccttggcctt ctccaccacg cgctcggcga tcgagcgcac gtggcggtcg gagttgccgg
     6421 atgcgatgac catcgtatcg gcaatgtcgg tcagccctcg tacatccaga acccttacgt
     6481 tgacggcctt catgtcatcg agcgctgtcg tcacggcatc cttcagggac gagacgcgct
     6541 ccgcagcggg gcgcaggcgt gtacgggtgg tcatgtgctt aagtggtgtc tcctgatggc
     6601 ctgaggtcat ggaccttgct gtgcagcata gcacctcgtc tcgagaatga tctgccgtac
     6661 tgcgtccgga acgagatagc ggggatcgcg accggcgacc agagcggcgc gcagctcggt
     6721 ggaggagatc tcgagctgcg tcacggcgtg cacgtacagg cacccggcgg gccgctcgtg
     6781 cagctcgcgg atggcgcccg ttccgtggtc gaccatcacc tccccgagcg ggcccttgct
     6841 cggcgccttc cagcccgggc gctgcgcgac gacgatatgg gccagctcga gaagctcgcg
     6901 ccagcgatgc cagctcggca gaccgaggaa cgcatccatc ccgagcagca ggcagagcga
     6961 ccgctgcggg tattcgcggc gcagctccgt gagcgtgtcg acggagtacg agacgccgga
     7021 gcggcgcacc tcccggtcgt cgaccacgaa gcccggctga tcggcgatcg cgacttcgac
     7081 catcttcagg cgcagctccg cgctcgcgta caggtggtcg cggtgcggag ggctgccggt
     7141 cggcaggaag cgcacctcgg cgagcttgag ctcctgccac agctcgaacg cggtgcgcag
     7201 atgcccgcag tggatcgggt cgaaggtgcc gccgaaaagc ccgatgggct gctgcatggc
     7261 tagcgacagg gatgtcagag cccgagccaa ttcatactac gcaaagagcg ccctggtaag
     7321 gagcatgagc tcatcccagg cattgccggc gaggcggccc ttgatcacgc ggtcggtgcg
     7381 cgcggcgcgg gccgttagat cggtgaaggg catgtgcggc gcacgccgcg cggcagcctc
     7441 gagggccgcg ccctggcgct gccagccgcg cggcgcgcca gtgccgccga gcgctcccca
     7501 cgcatcatgc agcgccttgg tgagcgccca cagcacgagc gtcggctcgg tgccctcggc
     7561 ccgcagcccc gcgagcaccc gcagcgcgcg cgccgcagcg ccggatagca ccgcctccga
     7621 gagttgaaat acgtcgaaac gcgcgctgtc ggcagcgctg gcgagcacca tctcgggcgt
     7681 cacgggagca tcgccggcga ggagggcgag cttgttgagc tcctggtgcg cggcgagaag
     7741 attgccctcg gtacgctccg cgagcagctc gagggccgca tcgctcgccg cgagcttcag
     7801 ctgccgggcg cgcccgcgca gccagccgac gaagcgcgct gcgtccaccg gccagatcgg
     7861 cacccaggcg ccgtgcgcct cgacggcgcg cacccaatcg gcgccctgcg cctcgcggtc
     7921 gagccgtggc gtgaggatga ggaacagcgt gtcgggatcg tgcgcctcga ggagcgcacg
     7981 cagcgcgccg ttgccggccg cgcccggccg tgcgcccatg cggatctcga acacccggcg
     8041 tgcgccgaac agcgagagac tgcgcgccgc gccacgcacc tcgtcccagt cgccggcgcg
     8101 ctcggcgacg tgcacttccc gctcgctgaa gccggccgcg cgcgccgcgg cccgcacggc
     8161 gtccgccgcc tcgccggcga gcagcggctc atccccggag atgagatagg cgggcagcag
     8221 ccgctcgcgc aggtggttgg cgagagagtc ggaggtgagc ttcaaatgca atccgcgccg
     8281 gcggccaccg gcgcattatc aaccacccgc ggctcgccgg tcaggcccct agaactgcca
     8341 atcgagcccc ccgaatatcc cgcgccggtg cccccactgg ggcgcaaaca cgccgacccc
     8401 cgtgccgctg cggatcgcgt aggccttgtc gaacaggttg atgacgtcga ggcgtgccgt
     8461 gagcgggccc gagcccgcca
//
LOCUS       DATLKL010000847         3492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_224260, whole genome shotgun sequence.
ACCESSION   DATLKL010000847 DATLKL010000000
VERSION     DATLKL010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224260"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VM713_11555"
     CDS             <1..753
                     /locus_tag="VM713_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HVH84938.1"
                     /translation="GSLLPPVLLDDDEAVAVAVAVRAAAGSVGKMEETAAGLLAKLDQ
                     LLPARLRKRASALHSVTVSLASARALPSMEVLTRIATACRDHLKLRLNYRDRAGNVTT
                     RTVEPLRLAHTGHLWYLVAWDAQRADWRTFRTDRIQRLVASGPHFAPRKFPGDVAQFV
                     ARAMTQVPYRYRMRLKLEGSAAELVRRIPSWCGVLEALDDVSCTLTTGADSLEGLAAH
                     IVLIGTDFEILDAPELLPELRKIADRLYRATR"
     gene            770..1435
                     /locus_tag="VM713_11560"
     CDS             770..1435
                     /locus_tag="VM713_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVH84939.1"
                     /translation="MRVLIVEDDETIASTMSRGLTLLGYVVDHVPSAEAASAALLSEQ
                     FDLAIVDLGLPQEDGTSLVRRIRRTNKTLPVVVVTARDSIADRVDTLDMGADDYLVKP
                     VALAELLARCRAHIRRARAVASSKVQIGALAVDLEGRRAMVAGEALDLTRREWLLLEY
                     LAVNAGRIVSKERLLDAISSAPQDLSLNSVEAVVSRLRAKLGEAARIRTIRGLGYRLD
                     DEP"
     gene            1439..2824
                     /locus_tag="VM713_11565"
     CDS             1439..2824
                     /locus_tag="VM713_11565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVH84940.1"
                     /translation="MIRPLWSTIRGRLLAYLLPPLVLLLAAGVYLDYETGAAPVRAAY
                     DHALAAAALAIATHAESGTQPAELARIASAIDPLLHPAAGARAYFVVHAGGRYLAGDA
                     DLPVATLGARNPVFFDARFRGEAVRVVTYSLTGAQPLSVSVAETTAERDRATLRLLVS
                     EVLTDIVQLVATVVLVWIGVALALRPMLRLGEQIATRSPRELSPLDADTAPGEARLLV
                     TELNRLFAALGEASRVQQHFLANAAHQMRTPLAGLLIQLDLLIAEAADGRVREELLAL
                     RQSAQNLAHTADQLLTLARADPAVHLANEFTAVDCGKLITDVVAQNLDRALVQGIDLG
                     AQIEPVRLAGVAWLIREVLTNLVDNAIRYVPAGGHVTVRCGIAGGLAYLEVEDDGPGI
                     PPAERQAVRQRFYRLPNTPGSGCGLGLAIADEVARLHGAELEIGDCDGVGTRVRLRFP
                     QARGPLSTAAR"
     gene            3020..>3492
                     /locus_tag="VM713_11570"
     CDS             3020..>3492
                     /locus_tag="VM713_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84941.1"
                     /translation="MALEPGQVEELRIEAVQTHAFPLEKHAVGSISFDEDPAIVQAES
                     TLVSAAANLELSSKEHRRVQSLGEGNGIAQKELEQALSAQETAAAALKAARDAVRALG
                     KSDSEIDRMIGARRIQSTPAGRPRLKWALANVAESDSVAVRPGQPVSLAVMAYPER"
BASE COUNT          522 a         1177 c         1202 g          591 t
ORIGIN      
        1 ggctccttgc tcccgccggt gctgctcgat gacgacgagg cggtggcggt ggcagtcgcg
       61 gtacgcgccg cggccggcag cgtcggcaag atggaggaaa ccgccgccgg gctcctcgcc
      121 aagctcgatc agctgctgcc cgctcggctg cgcaaacggg cgagcgcctt gcactcggtc
      181 acagtctccc tggccagcgc gcgcgcactg ccgtccatgg aggtgctcac ccggatcgcc
      241 accgcctgcc gcgatcacct caaattgagg ctcaactatc gggaccgcgc cggaaatgtc
      301 acgacccgca cggtcgagcc gctgcgcctg gcgcacacgg ggcatctctg gtacctggtc
      361 gcctgggacg cgcagcgggc cgactggcgc accttccgca ccgatcgcat ccagcgcctc
      421 gtggccagcg gcccgcattt cgcgccgcgc aagttccctg gggatgtggc ccagttcgtg
      481 gcgcgcgcca tgacacaggt accgtaccgc tatcggatgc gactcaaact cgaaggatcc
      541 gccgcagagc tcgtgcgacg gattccgagc tggtgcggag tgttggaagc gctggacgat
      601 gtgagttgca ctctgaccac cggcgccgac tcccttgagg ggcttgcggc tcacatcgtg
      661 ctgatcggga cggacttcga gatcctcgac gcgcctgagc tccttccgga gctgcggaaa
      721 atcgccgacc gcctgtaccg ggcaacgcgc taaggcctaa gattgctcga tgcgagtcct
      781 gatcgtcgag gacgacgaga ccatcgccag caccatgagc cgcgggctga cgctgcttgg
      841 ttatgtggtg gatcatgtac cttcggccga agcggcctcg gcagccctgc tgagtgagca
      901 gttcgatctc gcgatcgtgg atcttggcct gccgcaggaa gacgggacga gtctggtgcg
      961 acgcattcgc cgcaccaaca agacgctgcc ggtcgtggtc gtgaccgcgc gcgattcgat
     1021 cgccgatcgc gtggacaccc tcgatatggg cgccgacgac tatctggtga agcccgtggc
     1081 ccttgccgag ctcctcgccc gctgccgggc gcacattcgc agggctcgcg ccgtcgccag
     1141 ctccaaggtt cagatcggcg cgctcgctgt cgacctcgag gggcgccggg ccatggtggc
     1201 cggcgaagcc ctggacctga cgcggcgcga gtggttgctc ctcgagtatc tcgcggtcaa
     1261 cgccggtcgt atcgtcagca aagagcggct cctggatgcc atcagctccg cgccgcagga
     1321 tctctcgctc aattccgtcg aagcggtcgt ctcacggctg cgcgccaagc tgggcgaggc
     1381 ggccaggatc cgcacgattc gcggcctggg ttaccggctc gacgacgagc cttaggacgt
     1441 gatccgtcct ctctggagca cgattcgcgg tcggctgctc gcctacctgc tgccgccact
     1501 agtgctgctg ctcgccgcgg gcgtctatct ggactatgag accggcgcgg cgccggtgcg
     1561 cgcagcctac gatcacgcgc tggccgccgc tgcactcgcc atcgccacgc acgccgagag
     1621 cggcacacag ccggcggaac tggcgcgcat cgcatccgcc atcgacccgc tgctgcatcc
     1681 ggcggcaggc gcgcgcgctt acttcgtagt acacgctggt ggacgttacc tcgcgggcga
     1741 tgctgacctc ccggtcgcga ctctcggtgc caggaatccc gtcttcttcg acgcgcgctt
     1801 tcgcggggaa gcggtgcgcg tcgtcacata ctcgctgacg ggagcgcagc cactctcggt
     1861 gtcggtggcc gaaaccaccg cggagcgcga tcgcgccacc ctgcggctgc tggtgagcga
     1921 ggtgctcacc gacatcgtgc agctcgtcgc gacggtggtg ctggtgtgga tcggggtcgc
     1981 gctggcgctg cgaccgatgc tgcggctcgg ggagcagatc gccacgcgct caccgcggga
     2041 gttgtcgcca ctcgatgcgg ataccgctcc cggtgaagcg cggttgctgg tcaccgagct
     2101 gaaccggctg ttcgctgccc tgggagaggc ttcgcgcgtg cagcaacatt tcctcgccaa
     2161 cgctgcgcat cagatgcgca caccactcgc cgggctcctg atccagctcg atctgctgat
     2221 cgctgaggcg gctgacggcc gtgtgcgcga ggagctgttg gcactgcgcc agagcgcgca
     2281 gaaccttgcg cacactgccg atcagctgct gacgctcgcc cgcgcggatc cggcggtaca
     2341 tctggcgaac gaattcaccg cggtcgactg cggcaagctc attacggatg tcgtggcgca
     2401 gaacctcgat cgcgcactcg tccaaggcat cgatctcgga gcccagatcg agccggtgcg
     2461 tctcgcgggc gtcgcatggc tgattcgcga ggtgcttacg aacctggtcg acaacgccat
     2521 acgttatgtc cccgcgggag gacacgtgac cgtgcgctgc ggcatcgccg ggggcctcgc
     2581 gtacctcgag gttgaggacg atggcccggg gatcccaccc gctgagcgtc aggcggttcg
     2641 ccagcgcttc tatcgcttgc ccaatactcc gggcagcgga tgtggcttgg gacttgcgat
     2701 tgccgatgag gtggcgcgcc tgcatggggc ggagctggag atcggggact gtgacggagt
     2761 tggcactcgg gtgcgtctgc ggtttccgca agcgcggggg ccgctctcga cggctgcccg
     2821 atagcgggtt tgtcagcttc gcggcaggta cggcgggcat tatttcccga tgccggcggt
     2881 accgaagcgc ctggtagccc gggcggccct cgcgcttggc tctttaggcg ttgtctccgt
     2941 ttccatccag ctcgctgcct ctccgaaggg gtctctggcg gcgccgcctt cgcacgccgc
     3001 tgtggcgcgc gcggccagcg tggccctcga gcccggccag gtcgaggagc tgcgcatcga
     3061 ggccgtgcaa acccacgcct ttccgctcga gaagcacgcg gttggcagca tcagcttcga
     3121 cgaggacccg gcgatcgtgc aggcggagtc cacgttggtc tctgccgcgg ccaacctcga
     3181 attgagcagc aaggagcaca ggcgcgtgca gtcactgggt gagggcaacg gcattgcgca
     3241 gaaggagctc gagcaggcgc tgtcggcgca ggagaccgcg gcggccgccc tcaaggccgc
     3301 gcgcgatgcg gtgcgcgcgc tcggcaagag cgactctgaa atcgaccgca tgatcggcgc
     3361 gcggcggatt caaagcacgc ccgccgggcg cccgcggctc aagtgggcgc tcgccaacgt
     3421 cgccgagagc gacagtgtcg cggtgcggcc gggacagccg gtgtcgctag cggtgatggc
     3481 ctatcccgag cg
//
LOCUS       DATLKL010000848         1849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_21555, whole genome shotgun sequence.
ACCESSION   DATLKL010000848 DATLKL010000000
VERSION     DATLKL010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21555"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VM713_11575"
     CDS             complement(<1..655)
                     /locus_tag="VM713_11575"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84942.1"
                     /translation="MAGGAHAPAQYVVVGANHRSSSVTFRDMIFVDDAAAPALLRGLG
                     ERGVAQALLLSTCDRVEVQGVAADPDAAHDAVAAVIAARAGLDPAMLKRQLYRLSAEE
                     AVRHIFAVAASLESAIVGEPQVLGQVKASHRLARDAGTVGSELEAALQAAYGAAKRVR
                     TETAIAAVPVSIVATAVEVAREVHGELGALAGLLIGTGDMDQLMAEELQRAGLGRLTV
                     "
     gene            complement(663..>1849)
                     /gene="hisS"
                     /locus_tag="VM713_11580"
     CDS             complement(663..>1849)
                     /gene="hisS"
                     /locus_tag="VM713_11580"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HVH84943.1"
                     /translation="YEEIATPIFEFTEVFQRTLGDTSDIVTKEMYTFTDRGGEQITLR
                     PEATAGIMRALISGGLSQNLPLKFFCAGPMFRYERPQKGRYRQFHQIDIELLGVPDPL
                     GDVEVIAVGAHILDALGILGKATLELNTLGDSESRTAYRKALVGYLAKHRDGLSADSR
                     SRLERNPLRILDSKDEGDAKIVANAPIFFDYLNESSRDFFKKVQASLDALGIAYRLNP
                     RVVRGLDYYCHTVFEFTTTALGAQGAVIAGGRYDGLAETMGGPATPATGWAGGVERLA
                     MLMSDAGTRLRPVAVIPVGPEAEAVALRVARDLRYAGITVELEFHGSLSRRMKRANKI
                     GARAAVLLGADELKRGAATLRYLDSGAQQVVPFDGLARVLVDPGGESQLVAQNAQLRE
                     EH"
BASE COUNT          290 a          647 c          617 g          295 t
ORIGIN      
        1 ccacggtgag gcggccgagg cccgcgcgct ggagttcctc agccatcaat tggtccatgt
       61 cgccggtccc gatcaggagt ccggcgagtg cgccgagctc gccgtgcacc tcgcgggcca
      121 cctcgactgc ggtcgccacg atcgagacgg gtacggccgc gatggcggtc tcggtccgca
      181 cgcgcttagc cgcgccgtaa gcggcctgga gggccgcctc gagctcggat ccaactgttc
      241 ccgcgtcccg cgcgaggcga tgcgatgcct tgacctggcc cagcacctgg ggctctccga
      301 caatagcgct ttccagggaa gcggccaccg caaagatgtg gcgcaccgct tcctcggcgc
      361 tcaagcgata gagctgtctc ttgagcattg ccggatcgag cccagcacgc gcagcgatca
      421 ccgccgcgac tgcgtcgtgc gcggcgtccg ggtcggcagc aactccctgc acctcgaccc
      481 gatcgcaggt cgacagcagg agtgcctgcg cgacaccgcg ctcgccgagc ccgcgcaaca
      541 aggcgggggc ggccgcgtcg tcgacgaaga tcatgtcgcg gaatgtcacc gagctcgatc
      601 gatggttggc gccgaccacc acatactggg ccggcgcgtg tgcgccgccg gccatggtcg
      661 cgctagtgct cctctctgag ctgagcattc tgggcgacga gctgcgactc gccgccgggg
      721 tcgaccaaga ccctcgccaa gccgtcgaac ggcaccacct gctgcgcgcc gctgtcgaga
      781 taacgcagcg tcgccgctcc gcgcttcagc tcgtccgcgc cgagcaggac ggccgcccgc
      841 gcgccgatct tgttggcgcg cttcatgcgc cgcgagagac tgccgtggaa ctcgagctcg
      901 accgtgatgc cggcgtagcg caggtcccgc gcgacgcgca gcgcgaccgc ctcagcctcc
      961 gggccgaccg ggatcacggc aaccggccgc aggcgcgtcc ccgcgtcgct catcagcatt
     1021 gccaggcgct ccacaccgcc cgcccagccg gtggccggcg tcgccggtcc gcccatggtt
     1081 tcagcgagcc cgtcataccg gccgcccgcg atcaccgcac cctgcgcgcc cagggccgtc
     1141 gtcgtgaact cgaacaccgt gtggcagtaa tagtcgaggc cgcgcacgac acgcgggttg
     1201 agccgatagg caatgccgag ggcatcgagc gaggcctgca ctttcttgaa gaagtcacgc
     1261 gacgactcgt tcaaatagtc gaagaagatc ggcgcgttgg cgacgatctt cgcgtccccc
     1321 tcgtccttgg agtcgaggat gcggagcgga ttgcgctcga gccggctgcg gctgtccgct
     1381 gaaagcccgt cgcggtgttt ggcgagatat ccgacgagcg ccttgcggta ggcggtccgg
     1441 ctttccgagt cgccgagcgt gttcagctcg agcgtggcct tgccgagaat gccgagcgcg
     1501 tcgaggatgt gcgcgccgac cgcgatcacc tcgacatcgc caagcgggtc cggcacgccc
     1561 agcaactcaa tgtcgatctg atggaactgg cggtaccgtc ccttctgcgg acgctcatag
     1621 cggaacatgg ggccggcgca gaagaatttc agcggcaggt tctggctgag gccacccgag
     1681 atgagcgcac gcatgattcc cgccgtcgcc tccgggcgca gggtgatctg ctccccgccg
     1741 cggtcggtga aggtgtacat ctccttggtg acgatatcgc tggtgtcgcc gagggtgcgt
     1801 tggaacacct ccgtgaattc gaagatcggc gtcgcgatct cctcgtagc
//
LOCUS       DATLKL010000849         3257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_33023, whole genome shotgun sequence.
ACCESSION   DATLKL010000849 DATLKL010000000
VERSION     DATLKL010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33023"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..981)
                     /locus_tag="VM713_11585"
     CDS             complement(31..981)
                     /locus_tag="VM713_11585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVH84944.1"
                     /translation="MKAHACAALAYLAMLLLGAAARPSMAAPAAPAAPAAPPVKPQTT
                     AEVLSSSSPSDWRPLDPQNTLYLELPTGRVIIEMAPVFAPNHVANVTTLAREGYFDGL
                     AFMRSQDNYVVQWGDAQGRKPLQKAQRTLPAEFERPLRGLTLARISDPDTYAPLVGFL
                     DGFPVAADPQTARAWLVHCYGMVGAGRDNDVDSGGGTELYVVIGQAPRHLDRNVTLLG
                     HVVRGMELLTVLPRGHGALGFYEPSESGMLIRSLRLAADLPEGERTALEELRTDTPTF
                     AAFVEARRNRHEEWFKVPAGRIDVCNIPVPVRPVAGAKGP"
     gene            complement(996..2792)
                     /locus_tag="VM713_11590"
     CDS             complement(996..2792)
                     /locus_tag="VM713_11590"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVH84945.1"
                     /translation="MQRRDLLKLGLSGSLALSAPAWLAARVMSPFERVRPGDPYWPTA
                     AQWQELKVAVGGNLLEPHALFAPCATTGPGVSAACTDVAQNLRNPFWIGDQPGGTQVS
                     GWLDAWTPAASAYAVKARNSVDVARAVTFARTHNLRLAVKGGAHSYLGTSNAPDSLLI
                     WTRALNEVRLHDDFVGVGCAGKVAPVPAVSLGAGCMWIDAYTAVTTHAGRYVQGGGCT
                     SVGVAGLVQSGGFGSFSKGFGNAAAGLLEAEVVTADGQVRIVNACRDPDLLWALKGGG
                     GGSFGVVTRLTLRTHALPEFLGSAWGTVRAQSDSAFRALIARFIAHYAERLANPHWGE
                     QVQVKSDNSLKISMVQQGLAREEARAAWQPFFDWVKASADLKVSDELGASGHQAQSWW
                     EVQGNPSMIQDKRPGASPDHGYWQGDQDQVGAFLHGYDSLWLPAALLESAAQARLGAA
                     LFAASRHKDVELHFNKGLAFAAPEGVKASLDTAMNPGVTEAFALAIIADGERPSYPGE
                     ARPPMDLEAAHGDARAIDAATAELRKIAPQAGSYLSESNFFNERWQQDYFGAHYPRLK
                     AIKKQYDPEGLFIVHHGVGSEDWSADGFTRLK"
     gene            complement(2848..>3257)
                     /locus_tag="VM713_11595"
     CDS             complement(2848..>3257)
                     /locus_tag="VM713_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016142195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HVH84946.1"
                     /translation="LLRSRGVTVKFAIHPVAGRMPGHMNVLLAEAGVPYDLIADLDEI
                     NAEFDTADVALIIGANDVANPVARTDKSSPIYGMPILNADKAKNVLVIKRGKGAGFSG
                     IENELFYLDNTRMVYGDAQKVVTDLIAAVKAAG"
BASE COUNT          474 a         1120 c         1148 g          515 t
ORIGIN      
        1 tcgcgtccgc cgaagggcgg acgctagagc tcaggggcct ttggcgcccg ccacgggccg
       61 cactggcact gggatattgc acacgtcgat ccggcccgcc ggcaccttga accattcctc
      121 gtggcggttg cgccgcgcct cgacgaaagc ggcgaaggtc ggcgtatcgg tgcgcagctc
      181 ctcgagcgcg gtgcgctccc cctcggggag atccgcggcg aggcgcaacg agcggatcag
      241 catgccgctc tccgagggct cgtagaagcc gagtgcgccg tggccgcgcg gcagcacggt
      301 gaggagctcc atgccgcgca ccacgtgtcc gaggagcgtc acgttgcggt cgaggtgacg
      361 cggcgcctgg ccgatcacga cgtagagctc cgtgccgccg cccgagtcga cgtcattgtc
      421 gcgcccggcg ccgaccatgc cgtagcagtg cacgagccag gcgcgggccg tctgcgggtc
      481 cgcagctacg gggaagccgt cgaggaaacc gaccagcggc gcgtaggtgt caggatcgct
      541 gatgcgcgcg agcgtgagcc cgcgcagcgg ccgctcgaac tcggcgggga gcgtccgctg
      601 cgccttctgc agcggcttcc tgccctgcgc atctccccac tgcaccacgt agttgtcctg
      661 ggagcgcatg aacgcgaggc cgtcgaaata gccctcgcgg gcgagcgttg tgacgttcgc
      721 gacgtggttc ggagcgaata ccggggccat ctcgatgatc acgcgcccgg tggggagctc
      781 caggtacagc gtgttctgcg ggtcgagcgg ccgccagtcc gagggcgagg aggagctgag
      841 aacctccgcg gtcgtttgcg gcttgacggg cggagcggcc ggagccgcgg gagcggcggg
      901 cgctgccatt gacggccgcg cggccgcgcc gaggagcagc atcgcgaggt atgccagcgc
      961 tgcgcaggcg tgtgctttca tgacttttcc ctcgctcact tgagccgtgt gaagccgtcc
     1021 gcgctccagt cctcgctccc cacgccatgg tgcacgatga agaggccctc ggggtcgtac
     1081 tgcttcttga tcgccttcag gcgcgggtaa tgtgcgccga agtagtcctg ctgccagcgc
     1141 tcgttgaaga aattgctctc cgagagataa gagccggcct gcggtgcgat cttgcgcagc
     1201 tccgcggtcg ccgcgtcgat cgcgcgcgcg tccccgtgcg cggcttcgag atccatcggc
     1261 ggacgtgcct cgccgggata ggaggggcgc tcgccgtcag cgatgatcgc gagcgcgaag
     1321 gcttcggtga cgccagggtt catggccgtg tcgagcgagg ccttgacgcc ctcgggcgcg
     1381 gcgaacgcga gccccttgtt gaagtgcagc tccacgtctt tgtggcgcga ggcggcgaac
     1441 agcgccgcgc cgaggcgcgc ctgcgcggcg ctctcgagga gtgcggcggg gagccacagc
     1501 gagtcgtaac cgtgaaggaa ggcgccgacc tgatcctggt ctccctgcca gtagccgtga
     1561 tccggtgagg cgccgggccg cttgtcctga atcatgctcg ggttgccctg aacctcccac
     1621 cagctctgcg cctggtggcc gctggcgccg agctcgtcgc tgaccttgag gtccgcggat
     1681 gccttcaccc agtcgaagaa cggctgccac gcagcgcgcg cctcctcgcg cgcgaggccc
     1741 tgctgcacca tggagatctt gagagagttg tcgctcttca cttgcacctg ctcgccccag
     1801 tgcgggttgg cgagccgctc ggcgtagtga gcgatgaagc gcgcgatcag cgcacgaaac
     1861 gcgctatcgc tctgcgcccg aaccgtgccc caggcgctcc cgaggaattc cggcagcgcg
     1921 tgcgtgcgca gcgtcagccg cgtcactacg ccgaagcttc cgccaccgcc gcccttcaac
     1981 gcccagagga gatccggatc gcggcaggcg ttgacgatcc ggacctggcc gtcggcggtc
     2041 acgacctccg cctcgaggag ccctgcggcg gcgttgccga agcccttgga gaagctgccg
     2101 aagccgcccg actgcacgag cccggcgacg ccgactgagg tgcagccgcc cccctgcacg
     2161 tagcgccccg cgtgtgtggt gactgccgta taggcatcga tccacatgca gcccgcgccg
     2221 agggagactg ccgggactgg cgcgactttg cccgcgcagc ccacgcccac gaagtcatcg
     2281 tgcagccgaa cctcgttgag cgcgcgcgtc cagatgagga gcgagtccgg cgcgttcgag
     2341 gtgccgaggt agctgtgcgc gccgcccttt accgccaggc gcaggttgtg ggtgcgcgcg
     2401 aacgtcacgg cgcgggcgac gtctacggag ttgcgcgcct tcacggcgta agcgctggcc
     2461 gcgggcgtcc aggcatcgag ccagccggag acctgcgtgc cgcccggctg atcgccgatc
     2521 cagaaggggt tacgcaggtt ctgcgccacg tcggtgcacg cggcgctgac tccgggtcct
     2581 gtggtcgcgc acggcgcgaa cagggcgtgc ggctcaagga gattgccgcc gaccgcgacc
     2641 ttgagctcct gccactgcgc agcggtcggc cagtaaggat cgcctggccg cacgcgctcg
     2701 aaaggcgaca tgacgcgcgc cgcgagccac gcgggcgcgc tcagcgccag cgaccccgag
     2761 agcccgagct tcaacagatc ccggcgctgc atcgcgccac ctccctgagg tccctgcctg
     2821 cgcggccgag ggcggtcggc gtcagcttca gcccgccgcc ttcaccgccg cgatcaggtc
     2881 cgtcaccacc ttctgtgcgt cgccgtacac catgcgcgtg ttgtcgaggt agaagagctc
     2941 gttctcgatt cccgagaagc ccgcgccctt gccgcgcttg atgacgagga cgttcttcgc
     3001 cttgtcggcg ttcaggatcg gcatgccgta gatcgggctc gacttgtcgg tgcgcgccac
     3061 cgggttcgcg acgtcgttgg cgccgatgat gagggccacg tccgcggtgt cgaactccgc
     3121 gttgatctca tcgagatcgg cgatcaggtc gtaggggact cccgcctcgg ccagcagcac
     3181 gttcatgtgg ccgggcatgc gtcccgccac cgggtggatg gcgaacttaa cggtcacgcc
     3241 gcgggaccgt agcagct
//
LOCUS       DATLKL010000850         1585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_66857, whole genome shotgun sequence.
ACCESSION   DATLKL010000850 DATLKL010000000
VERSION     DATLKL010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66857"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VM713_11600"
     CDS             complement(<1..650)
                     /locus_tag="VM713_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HVH84947.1"
                     /translation="MKLWGGRFRSAPSDVFERFSASLDFDRRLIDADIRGSLAFARAL
                     ERAGILTASERETIVSAFEAIRAEAAAPAFFANATDEDVHTLVIRKLKERAGAVADKI
                     HTGRSRNEQVSLDARLWLCEQCDRMRALLGAVMRALLDLGEKYPDAVIPGYTHLRRAQ
                     AVLWPHYLLAYFEMFARDCERFAEVKRHTDRLPLGSGALAGSGFPFDREAIAQDLG"
     gene            complement(647..>1585)
                     /locus_tag="VM713_11605"
     CDS             complement(647..>1585)
                     /locus_tag="VM713_11605"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HVH84948.1"
                     /translation="RPLLAKKQVEAALATGCDGLAHGCTGKGNDQVRFELAYKALAPD
                     LAVIAPWREWKIASREDAIEYARRHNVPISASSTKIYSRDRNIWHISHEGGALEDPAN
                     RAPDDIRMLTKSPSEAPNTPGEVTLAFERGVPVSVNGKTMSAVELLETLNQIGGAHGV
                     GRIDLVENRFVGMKSRGCYETPGGTLILFAMRELEALTLDKSTLHYKQKLALDYAEMV
                     YNGLWFTPLREALDAFFETTCAPVTGEVKLRLYKGSIEPLSRKSPHSLYSLDMASFTM
                     GASYDQKDALGFINLVGLPIRVRAGLRIEASAGKAK"
BASE COUNT          273 a          550 c          480 g          282 t
ORIGIN      
        1 aaacccagat cctgggcgat ggcttcgcgg tcaaacggaa agccactgcc agccagcgcc
       61 ccggagccca acggcaatcg gtcggtgtgc cgcttcacct ccgcgaagcg ttcgcagtcg
      121 cgcgcaaaca tttcaaaata ggccagcagg taatgcggcc agagcacggc ctgggcccgg
      181 cgcaggtgag tatagccagg gatcaccgcg tccgggtact tctcccccag atcgagcaac
      241 gcgcgcatga cggcgccgag cagggcgcgc atccggtcgc attgctcgca caaccacagc
      301 cgcgcgtcga gcgacacctg ctcgttgcgg ctgcggccgg tgtggatctt gtccgccacc
      361 gcacccgccc gctccttgag cttgcggatc accaaggtgt gcacgtcttc gtcggtcgcg
      421 ttggcgaaaa atgcgggagc cgcggcctcg gcgcgaatcg cctcaaaggc cgagacgatg
      481 gtctctcgct ccgaggcggt caaaataccg gccctctcga gcgcccgcgc gaacgccagc
      541 gaaccgcgaa tatcggcgtc aatcaaccgc cggtcaaagt cgagtgacgc ggagaagcgc
      601 tcgaagacgt ccgacggcgc cgaccgaaac cgcccgcccc agagcttcat ttcgctttgc
      661 cggccgaggc ttcgatcctc aacccagccc gaacccgtat cggcagcccg accaggttga
      721 tgaagcccag cgcgtctttc tggtcgtagc tggcgcccat cgtgaagctc gccatgtcga
      781 gcgagtacag cgaatggggc gacttccggc taagcggctc gatcgagcct ttgtatagtc
      841 gaagcttcac ttcgcccgtg accggcgcgc acgtggtttc gaagaacgca tcgagcgcct
      901 cgcgcagcgg cgtgaaccat aggccgttgt acaccatctc ggcgtagtcg agcgccagct
      961 tctgcttgta gtgcagggtg ctcttgtcga gcgtcagggc ctccagctcg cgcatcgcaa
     1021 acaagatcag cgtgccgccc ggtgtctcgt agcacccccg cgatttcatc cccacgaacc
     1081 ggttctcgac caggtcgatc cggccgacgc cgtgggcgcc cccgatctgg ttgagcgtct
     1141 cgagcaactc gaccgcgctc atcgtcttgc cgtttaccga caccggaacc ccgcgctcga
     1201 acgcaagcgt cacctcgccc ggcgtgttgg gcgcctctga cgggctcttg gtgagcatcc
     1261 ggatgtcatc cggcgcccga ttggctggat cttccagcgc gccgccttcg tgcgatatat
     1321 gccagatgtt gcggtcgcgc gagtagatct tcgtgctcga agcggaaatc ggcacgttgt
     1381 gccggcgcgc atattcgatg gcatcctcgc ggctcgcgat cttccattcc cgccacggag
     1441 cgatcaccgc caagtcggga gccagcgctt tgtaggccag ctcgaaccga acctggtcgt
     1501 ttcccttccc cgtgcagccg tgcgccagtc cgtcgcatcc ggtcgcgagt gccgcttcca
     1561 cctgcttttt cgccagcagg ggccg
//
LOCUS       DATLKL010000851         2999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_213313, whole genome shotgun sequence.
ACCESSION   DATLKL010000851 DATLKL010000000
VERSION     DATLKL010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213313"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="VM713_11610"
     CDS             <1..1337
                     /locus_tag="VM713_11610"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVH84949.1"
                     /translation="IDDSEAVRTAFEVLLSLHGARVLGAGTPDAGLALLAREPIDLVI
                     QDMNFQREATSGVEGVALFREVRTRHPHVPTILLTAWTHLETAVELVRAGAADYVAKP
                     WDDTRLLTSVRNLLDLASARAEAEALRARDREARASLAARFDLKGVVYESEAMHALVT
                     VATQVAHAEVPVLITGPNGVGKEVLADIVQGNSERAGQSYFKVNLGALPHELIEAELF
                     GTEAGAFTGARARVGRFEAADGGTLFLDELATLSPGGQAKLLRVLQTGEFERLGANTT
                     RRTRVRVIAATNTNLRQAILAGTFREDLFYRLAVIELEVPPLAERRADILPLARHFLE
                     PGFELTPEAGAALQRHSWPGNVRELQNLIRRACLLSGSRAIGAAALGLPATVAAVPDS
                     EPDRAAVVAALERARGVITQAARDLGLSRQALYRRMEKLGLRTDAARPPAGA"
     gene            1337..2635
                     /locus_tag="VM713_11615"
     CDS             1337..2635
                     /locus_tag="VM713_11615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH84950.1"
                     /translation="MPRWSLAGRLAGALALTAAVAAGVSACATRWLPPLPAALVGFAA
                     ALPVGLWLARQAPRRWVRVLQAVRDGIVSLRDRDFSVSVAAVEDAELSGLIGAYNSLG
                     DLLRRERLDLYQRELLLDTVIQTTPLSMVLTDDGGRIVYSNAAARQLLRAGRKLEGLP
                     LTVVLSDAPPALAAALGAGGDTLFTMDVGGEPQVYHLSQRGFVLNARPHRLMLLKQLT
                     RELAAQEVAVWKKVLRVIAHELNNSLAPITSLAHSGRLLAGEAADARLKRVFDTIGER
                     AAHLATFIDGYARFAKLPRPRPEPVAWAPFLARLEGTTPFRVEGTLPPAPGSFDARQL
                     EQVMINLLKNAAESGSPPGEITLAVQHTAPGFRIEVADRGTGLTEGTLRDALLPFYST
                     KAAGTGLGLTLCREIVDAHGGRLSLANRAGGGALVTVWLP"
     gene            complement(2538..>2999)
                     /locus_tag="VM713_11620"
     CDS             complement(2538..>2999)
                     /locus_tag="VM713_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HVH84951.1"
                     /translation="TEKYGIVAVEGENATTSRVRSIVEKPKPADAPSNLAVVGRYVLA
                     PAIFEHLEKLGQGAGGEIQLTDGIAALMREEAVYAYRFSGTRYDCGSKLGYLQATVEY
                     ALGHEELGGQFRKYLERFALTAATQSPARRRPRGSQGSSARRARRLSRGRA"
BASE COUNT          406 a         1113 c         1029 g          451 t
ORIGIN      
        1 tgatcgacga cagcgaggcg gtgcgcaccg ccttcgaggt gctcttgtcg ctgcacggcg
       61 cgcgggtact cggcgcaggc actcccgacg ctggcctggc gctgctcgcg cgcgagccga
      121 tcgacctcgt catccaggac atgaactttc agcgcgaggc gaccagcggc gtggaaggcg
      181 tcgcgctgtt tcgcgaggta cgcacgcgcc atccccatgt gccgaccatc ctgctgaccg
      241 cctggacgca tctcgagacg gccgtcgaac tggtgcgagc cggcgccgcc gactatgtcg
      301 ccaagccctg ggatgacacg cgcctgctca ccagcgtgcg caacctcctc gacctcgcca
      361 gcgcacgcgc cgaggccgag gcgctccgcg ccagagaccg cgaggctcgg gcgagcctgg
      421 cagcgcgctt cgacctgaag ggcgtcgtct acgagagcga agccatgcac gcgctcgtca
      481 ccgtggcgac gcaggtggcg catgccgagg taccggtgct catcaccggt cccaacggcg
      541 tcggcaagga ggtgctggcc gatatcgtgc agggcaactc cgagcgcgcc ggccagtcct
      601 acttcaaagt caatctcggg gcgctgccgc acgagctcat cgaggcggag ctgttcggca
      661 ccgaggcggg tgcgttcacg ggagcccgcg cgcgcgtcgg ccgcttcgag gccgccgatg
      721 gcggcacgct gttcctcgat gagctcgcga cgctctcccc cggcgggcag gcgaagctcc
      781 tgcgggtgtt gcagaccggg gagttcgagc gcctgggcgc caacaccacg cgtcgcacgc
      841 gcgtgcgcgt gatcgccgcc accaacacca acctgcgcca ggcgatcctt gcggggacct
      901 tccgcgagga cctcttctac cggctcgccg tcatcgagct cgaggtaccg ccgctcgccg
      961 agcggcgcgc ggacattctg ccgctcgcac gccacttcct cgagcccggg ttcgagctca
     1021 cccccgaggc gggcgcggcg ctgcagcgcc actcctggcc cggcaacgtg cgcgagctgc
     1081 agaacctcat caggcgcgcc tgcctgctct cgggcagccg cgccatcgga gctgccgcgc
     1141 tcggcctgcc ggcgaccgtc gccgccgtgc ccgacagcga gccggatcgc gccgccgtgg
     1201 tggccgcgct cgagcgtgcc cggggcgtga tcacccaggc cgcacgtgac ctcgggctgt
     1261 cccgtcaggc actctaccgg cgcatggaga agctgggttt gaggacagac gcagcacggc
     1321 cccccgcggg ggcctgatgc cgcgctggtc gctcgccggg cgcctggccg gcgcgctcgc
     1381 gctcactgcg gcggtcgccg cgggggtcag cgcctgcgcc actcgctggc tcccgcccct
     1441 ccccgccgcg ctggtcggtt tcgcagccgc gctgcccgtc ggcctgtggc tcgcgcgcca
     1501 ggcgccgcgc cgctgggtgc gggtgctgca agcggtgcgc gacggcatcg tgagcctgcg
     1561 cgatcgcgac ttcagcgtga gtgtcgcggc cgtggaggac gcggagctga gcggcctcat
     1621 cggcgcctac aactccctgg gcgatctgct gcgccgtgag cggctcgatc tctatcagcg
     1681 cgagctgctc ctcgacactg tcatccagac cacgccgctc tcgatggtgc tgaccgacga
     1741 tggcgggcgc atcgtctaca gcaacgccgc ggcgcgccag ttgctgcgcg ccggacgcaa
     1801 gctcgagggc ctgccgctca ccgtggtgct cagcgatgcg ccgccggcac tggccgccgc
     1861 tttgggtgcc ggcggcgata ctctctttac catggacgtc ggcggggagc cgcaggtcta
     1921 tcacctgtcg cagcgcggct tcgtgctgaa cgcccgcccg caccgcctga tgctcctcaa
     1981 acagctgacg cgcgagctcg ccgcgcagga agtcgccgtc tggaagaaag tgctccgcgt
     2041 catcgcccac gagctcaaca actcgctcgc ccccatcacc tcgctcgccc actccggacg
     2101 gctgctggcg ggcgaagcgg ccgacgcgcg cctcaagcgc gtcttcgaca ccatcggcga
     2161 gcgcgccgcg cacctcgcga ccttcatcga cggctacgcg cgcttcgcca agctcccgcg
     2221 tccgcggccc gagccggtgg cctgggcacc gttcctcgcc cggctcgagg gcacgacgcc
     2281 cttccgcgtc gagggaacgc taccccccgc gcccggcagc ttcgacgccc gccagctcga
     2341 gcaggtgatg atcaatctgc tgaagaacgc ggcggagtcc ggctcgccgc ccggggagat
     2401 cacgctggcc gtgcaacaca cggcgccggg cttccgtatc gaggtcgccg accgcggcac
     2461 gggcctcacg gaggggacgt tgcgcgatgc tttgctgccc ttctactcga ccaaagcggc
     2521 cggtacgggc ctggggctca cgctctgccg cgagatagtc gacgcgcacg gcgggcgctt
     2581 gagccttgcg aaccgcgcgg gcggcggcgc gctggtgact gtgtggctgc cgtgagcgcg
     2641 aagcgctcga ggtacttgcg gaactggccg ccgagctctt cgtggccgag cgcgtactcg
     2701 accgtcgcct gcaggtaacc gagcttgcta ccgcagtcat agcgcgtgcc gctgaagcgg
     2761 taggcataca ccgcttcctc gcgcatgagc gccgcgatgc cgtcggtgag ctggatctcc
     2821 ccgccggctc cctggccgag cttttccagg tgctcgaaaa tcgccggcgc cagcacgtag
     2881 cggccaacga cggcgagatt cgagggagca tcggccggct tgggcttctc gacgatgctg
     2941 cgcacgcggc tcgtcgtcgc gttctcgcct tcgacggcga cgatgccgta cttttcggt
//
LOCUS       DATLKL010000852         1647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_66893, whole genome shotgun sequence.
ACCESSION   DATLKL010000852 DATLKL010000000
VERSION     DATLKL010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66893"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VM713_11625"
     CDS             complement(<1..1121)
                     /locus_tag="VM713_11625"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HVH84952.1"
                     /translation="MLGAAALLLLLGVVATEAAAKPPAETTPSDRAHIAYSFAPVVRK
                     AAPAVVNIYTRTVIEQPGSPLFTDPFFSRFFGDQFFRGGPQRHTQNTLGSGVIVGADG
                     TIVTNQHVIKGADQITVVLSDRREFEAKLVGSDERTDIAVLKIDSGGTRLPVLQFGDS
                     DAIEVGDLVIAVGNPFAVGQTVTSGIISGLARTAVGVGDYDFFIQTDAAINPGNSGGP
                     LVTMDGKMVGLNTAIYSKSGGSVGIGFAIPSNMVRTVVASILAGGHVSRAWLGVEGRT
                     VTAEIADSLGLPRPSGVLVHDIYREGPASRAGLHPGDVILKVDGRPVDDAESLRYRVA
                     TKPVNTQAKLTIYRQGRQLVLPIALSPPPESVPREETELG"
     gene            complement(1273..>1647)
                     /gene="rplQ"
                     /locus_tag="VM713_11630"
     CDS             complement(1273..>1647)
                     /gene="rplQ"
                     /locus_tag="VM713_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010158120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HVH84953.1"
                     /translation="KAMFANLAAALIKHEQIKTTLPKAKDLRPVVEKLVTLGKRGGLH
                     ARRQALALLQDRKLTEKLFGPLAERYKDRAGGYTRVLRAGFRHGDAAAMAVIELVDRD
                     PAAKGQDSGPKPAAEAEAEAAA"
BASE COUNT          271 a          551 c          552 g          273 t
ORIGIN      
        1 ccgagctcgg tctcctcgcg cggcaccgat tcgggcggtg gcgaaagcgc aatcggtaga
       61 acgagctgcc gcccctgacg atagatcgtg agcttcgcct gggtgttgac cggcttggtc
      121 gcgacgcggt agcgaaggga ctccgcatcg tcgaccggcc ggccgtcgac cttgagaatc
      181 acgtcgcccg ggtgcaggcc ggcgcgcgac gcgggcccct ccctgtatat atcgtggacg
      241 agcacgcccg acggccgcgg cagcccaagg gagtcggcaa tctcggcggt gaccgtccgg
      301 ccttcgacgc ccagccaagc ccgcgagacg tgcccgcccg cgagtatgct cgcgacgacc
      361 gtgcggacca tgttggacgg gatcgcgaag ccgatgccga ccgatccccc cgacttcgaa
      421 tagatggcgg tgttaaggcc aaccatcttg ccatccatcg tgacgagcgg tccgcccgag
      481 ttgccggggt ttatcgcggc gtcggtctga atgaagaagt cgtagtcgcc gaccccgacg
      541 gccgtccgcg cgagccccga gatgatgccg ctcgtcacgg tctgcccgac tgcaaagggg
      601 ttgccgaccg cgatgacgag gtcgccaacc tcgatggcgt ccgagtcgcc gaactgcagc
      661 acgggaagcc gggtgccgcc ggaatcgatc ttcaagaccg cgatatcggt gcgttcgtcg
      721 gagccgacga gcttggcctc gaactcccgc cgatcggaga gaaccacagt gatctgatcg
      781 gcacctttga tcacgtgctg gttggtcacg atggtgccgt ccgccccgac gattacgccc
      841 gagccgagcg tgttctgggt atgccgctgg ggcccgccgc gaaagaattg atcgccaaag
      901 aagcggctga agaagggatc ggtaaagagc ggcgagcccg gctgctcgat cacggtgcgg
      961 gtgtagatat tgaccacggc gggcgccgcc ttcctcacca cgggcgcgaa gctataggcg
     1021 atgtgggcgc gatcggaggg tgtggtctcg gccggcggct tagcggcagc ctccgtcgcg
     1081 acgactccga gcaggagcag caacgcggct gcgccgagca ccgcgcgccg acccgtccaa
     1141 gccgtcgcta gcgccatgga tggccaaccc tttcgagatc gtccgtctcg atcattatgt
     1201 agtaagttcg aagcgaaagg gcagccgcgc cggctgccct cttgcgcatc ccttggggtc
     1261 gggccgcgcg gctcaggcgg ccgcctcggc ctccgcctcg gcggcaggct tcggacccga
     1321 atcttgtccc tttgctgccg ggtcgcgatc gaccagctcg atgaccgcca tcgccgccgc
     1381 gtcgccatga cgaaagccag cgcgaagcac ccgggtatat ccgccggcgc ggtccttgta
     1441 gcgctcggcg agcggcccga acagcttctc ggtcagcttg cggtcctgta gcaatgcgag
     1501 cgcctggcgc cgcgcatgca gcccgccgcg cttgccgagc gtgaccagct tctcgaccac
     1561 cggccgcaaa tccttggcct tgggcaaggt agtcttgatc tgctcgtgct tgatgagggc
     1621 ggccgccaga tttgcgaaca tcgcctt
//
LOCUS       DATLKL010000853         2264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_21587, whole genome shotgun sequence.
ACCESSION   DATLKL010000853 DATLKL010000000
VERSION     DATLKL010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21587"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VM713_11635"
     CDS             complement(<1..832)
                     /locus_tag="VM713_11635"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVH84954.1"
                     /translation="MPPANQNQLGSYLRDRRTRLDPAAFGFPMTRRRTAGLRREEVAH
                     RANVSPTWYTWLEQGRGGAPSPEVLERISRALMLTEVEREHLFLLGLGRPPEVRQRVT
                     EGVTPRLQRVLDALELSPAIVKNSTWDVLAWNRAAAAVLTDYGSLPPEQRNIVRLMFL
                     NPRVRAAQMDWDAVARFVVGAFRADAARAGTSRNAQALVEELCQRSPEFAGMWRDNDV
                     RSYGEGTKRIRHPRAGLLAFEYSAFSVDGRPDLGMVVYNPATQKDADRIRALLRRVTP
                     Q"
     gene            933..1823
                     /locus_tag="VM713_11640"
     CDS             933..1823
                     /locus_tag="VM713_11640"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVH84955.1"
                     /translation="MRVFVTGATGFIGSVITKELISAGHQVLGLARSDTAAASLEAAG
                     ADVHRGALEDRDSLRSGAAHADAVIHTAFIHDFSRFKEVSDIDRRAIETLGATLEGSD
                     RPLVITSGTGLVTPGRLATEDDAPTSTSIPRIASEEAASIVAGRGVRISVVRLPPSVH
                     GEGDHQFVPALIRTAREKGVSAYVGEGANRWPAVHRLDAAKLFKLALEKGTPGARYHG
                     AAEEGVPFRDIARVIGRHLNVPVVSNTPEAAASHFGWFAHFAALDNPTSSRQTREELG
                     WRPQQPGLIADLDQGHYFVE"
     gene            complement(1837..>2264)
                     /locus_tag="VM713_11645"
     CDS             complement(1837..>2264)
                     /locus_tag="VM713_11645"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVH84956.1"
                     /translation="ERLRHAVNVTIETRSQVVELAGDDHLNLVKIRDGEGNVVARPVG
                     GLFIMIGADPCTGWLKDAVRLDDRGFVLTGEDCGADAAVPFNLFQTNLAGVYAVGDVR
                     SGSVKRVASAVGEGSVVVQAIHARLASLRQAEAREPVTQ"
BASE COUNT          388 a          754 c          736 g          386 t
ORIGIN      
        1 cttggggagt tacgcgcctt aacagcgccc gaatccggtc ggcgtccttt tgggttgcgg
       61 ggttatagac gaccatgccc agatcgggac gcccgtccac gctgaaagcc gagtattcga
      121 acgcgagtag tccggcgcgc ggatgcctga tgcgtttggt gccttcgcca tagctgcgga
      181 catcattgtc gcgccacata ccggcgaact cgggactccg ctggcagagc tcctcgacca
      241 gcgcctgcgc gtttctcgag gtcccggcgc gcgccgcatc tgcgcggaaa gcgccgacga
      301 caaagcgcgc cactgcgtcc caatccatct gtgcggcgcg cacccggggg ttcaggaaca
      361 tgagccgcac gatgttgcgt tgttcgggag gaagggagcc gtagtcggtc aacacggccg
      421 cagcggcccg gttccacgcc agcacgtccc acgtcgagtt cttgacgatg gcgggactca
      481 gctcgagggc gtcgagcaca cgctgcagcc gtggcgtgac tccttcggtc acacgctggc
      541 gaacctcggg cgggcgtccg aggccgagaa gaaagagatg ctcgcgctcg acttccgtca
      601 gcatcagggc ccgggagatg cgctcgagca cctcggggga gggtgcccca ccacgtccct
      661 gctcgagcca cgtgtaccag gtcgggctga cattggcgcg gtgcgccact tcctcgcgcc
      721 gcagcccggc agtgcgccgg cgcgtcatcg gaaaaccgaa ggccgccggg tcgagtcggg
      781 tgcggcgatc cctcaggtac gagccgagct ggttttggtt cgcgggcggc atggttcaat
      841 ccaggtaatt actatacccg tataaactca ctactttacc cggatacagt acgcgcagag
      901 agtagccgcc gtctaccacg aggaccttac ttatgcgcgt gttcgttacc ggggcgacgg
      961 gattcatcgg ctccgtcatc accaaagagc tcatatccgc cggccaccag gtgctcgggc
     1021 ttgcccggtc agacaccgct gcggcatcac tcgaagccgc gggcgctgat gtgcaccgcg
     1081 gtgccctcga ggatcgcgac agcttgcgaa gcggcgcggc ccacgccgat gcggtgattc
     1141 ataccgcctt catccacgac ttctcgaggt tcaaggaggt gagtgacatc gaccggcgcg
     1201 ccatcgagac tctgggtgcc acgctcgagg gttccgatcg tccgctggtg atcacctccg
     1261 gcaccggact cgttacgccc ggccgcctcg cgaccgaaga cgatgccccg acctccacct
     1321 cgattccgcg cattgcctcc gaggaggcgg cctcgatcgt cgcggggcga ggagtgcgca
     1381 tctcggtcgt gcgcttaccg ccctcggtac atggtgaggg cgaccaccag tttgtgcccg
     1441 cgctcattcg cacggcgcgc gagaagggtg tatcggcgta tgttggagag ggggccaacc
     1501 gttggccggc cgtgcatcgg ctcgatgccg cgaagctctt caagctcgcg ctcgagaagg
     1561 gcacccctgg agcccggtac catggagccg ccgaggaggg cgtgccgttt cgtgacatcg
     1621 cacgggtcat cggcagacat ctcaacgtac ctgtcgtcag caacactccc gaggcggccg
     1681 ccagccactt cgggtggttc gcgcacttcg cagctctcga caacccgacc tcgagccggc
     1741 aaacgcgcga ggagctcggg tggcggccgc agcaacccgg cctgatcgct gatctggacc
     1801 agggccacta tttcgttgag tgagccgaag gcactgctac tgggtgactg gctcacgcgc
     1861 ttccgcctgc cgcagtgagg cgagtcgcgc gtgaatcgcc tgaacgacga ccgacccctc
     1921 gccgacggcg gaagcgactc tcttaactga gcccgagcgg acatcgccga ccgcgtaaac
     1981 cccggccagg ttggtctgaa agagattgaa ggggacggcc gcgtcggcgc cgcaatcttc
     2041 tccggtcagt acgaacccac ggtcatcgag gcgcaccgcg tccttcagcc acccggtgca
     2101 cggatctgcg ccgatcatga tgaagagccc gccgacgggc cgcgccacca cgttaccttc
     2161 gccatcgcgg atcttgacga ggttgaggtg gtcgtcgcct gcgagctcga caacctgcga
     2221 gcgggtctcg atcgtgacgt tcacggcatg ccgcaggcgt tcga
//
LOCUS       DATLKL010000854         2168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_123347, whole genome shotgun sequence.
ACCESSION   DATLKL010000854 DATLKL010000000
VERSION     DATLKL010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123347"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VM713_11650"
     CDS             <1..564
                     /locus_tag="VM713_11650"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVH84957.1"
                     /translation="LAENLADLAVMVRPPRDMDTVATPFAPHPYVIVAAPDHPLARVR
                     QIPRTALNRERFIQREHGSDTWNSMREVFGRQFPCLNIAMQIQSTETIKQAVVAGFGI
                     AFLSAHTLALDLRAGNLVVLDVQGFPAMLHWYLVHNRAKRLPPVATAFKEFLLRDGAA
                     LIERLVSMDLPGGERATRPARQSRKSQ"
     gene            complement(543..1250)
                     /locus_tag="VM713_11655"
     CDS             complement(543..1250)
                     /locus_tag="VM713_11655"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVH84958.1"
                     /translation="MAVARHRRGAGGLGLGPLNPMSLKDQAYVRIKEGIANTDIYSDR
                     QPLRLDERELMKALGVSRTPVREALSILEQEGFIRTVPRRGIFIVRKSKREMIEMIQM
                     WAALESMAARLATLTATDADIGKLRHLFDEFQSSPPAEHLDEYSDANIAFHQAIIRLG
                     GSRLIEDATHNLFLHVRAIRRATISQNNRAARSIIEHLKIIEALERRDTELAERLTRQ
                     HTLDLAAHVDRYCDFLD"
     gene            1751..>2168
                     /locus_tag="VM713_11660"
     CDS             1751..>2168
                     /locus_tag="VM713_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH84959.1"
                     /translation="MVTDPKPQSTSALARPWTQLIVGIICMVMVANCQYGWTLFVGPI
                     GDKFHWTKAAIQITFTLFVLFETWLVPVEGYIVDRIGPRWMIVGGGALAGLSWVLNSY
                     ADSLGMFYFNGIVGGIGAGAVYGTCIGNAVKWFPGRR"
BASE COUNT          365 a          698 c          682 g          423 t
ORIGIN      
        1 ctggccgaga atctcgccga cctcgcggtc atggtccgtc cgccgcgcga catggacacc
       61 gtggcgacgc ccttcgcacc gcatccctac gtgatcgtcg cggcgcccga tcacccgctg
      121 gcccgtgtgc ggcagattcc gcgcacggcg ttgaaccgcg agcgcttcat ccagcgcgag
      181 cacggttccg atacctggaa ctccatgcgc gaggtgttcg ggcggcaatt cccgtgcctc
      241 aacatcgcaa tgcagatcca gagcaccgag accatcaagc aggcagtggt ggccggcttc
      301 ggaattgcgt tcctctctgc tcatacgctg gcgctcgacc tgcgggccgg caacctggtg
      361 gtgctcgacg tgcagggctt ccctgccatg ctgcattggt atctggtaca caaccgcgcc
      421 aagcggctgc cgccggtcgc gaccgccttc aaggagttcc tgctgcgcga cggtgccgcg
      481 ctcatcgagc ggctggtgag catggatctg cccggcgggg agcgcgccac gcgcccggct
      541 cgtcagtcga ggaagtcgca gtagcgatcg acgtgcgcgg cgagatcgag ggtgtgctgg
      601 cgcgtcagcc gctcggcgag ctcggtatcg cgccgctcca gcgcctcgat gatcttcagg
      661 tgctcgatga tcgagcgcgc cgcgcgattg ttctgcgaga tcgtggcacg gcggatggcg
      721 cgcacatgca ggaacagatt gtgcgtcgcg tcctcgatga gccgcgagcc gccgaggcgg
      781 atgatcgcct gatgaaaggc gatgttggca tcggagtact cgtcgaggtg ctccgcgggc
      841 ggcgagctct ggaactcgtc gaacaggtgg cgaagcttac cgatgtcggc gtcggtggcc
      901 gtgagtgtcg cgagacgcgc cgccatgctc tcgagcgccg cccacatctg aatcatctcg
      961 atcatctcgc gcttggactt gcggacgatg aagatgccgc ggcgcggtac cgtcctgatg
     1021 aagccctcct gctcgagaat cgagagcgcc tcgcgcaccg gggtgcggct cacgccgagg
     1081 gccttcatga gctcgcgctc atcgaggcgg agcggctggc ggtcgctgta gatatcggtg
     1141 ttagcgatcc cctccttgat gcgcacgtag gcctggtcct tgagactcat gggattgagc
     1201 ggacccaggc ccaggccgcc ggctccccgg cggtgacggg ccaccgccat cgcgctcact
     1261 cgaccttgct cctgcgggaa tgccccaccg attcctcttc aggtcttgtc ccgggcccct
     1321 gattcattag aaggaacaag caaggagagt cccggaggct ataggttacg tcccgcgtcg
     1381 gtgccccgtt gcctcggaat ggtaacgcag tgtggatggc ataccacata tcagcagcgc
     1441 gcccggtgca cgcaattagt gcaatttcat ccgatcccgc gcagctcgcg gctcagtaat
     1501 gcacctcgca gccgcatttc cgtgcgcctt tgtcgtcatt acattctatt gacaagccca
     1561 agatatatca catatggtcg gccgccacct caagaactac ccatgcacca ttcgagggct
     1621 ttacggagcg gtcgaatgcc agcgggcgca ccgcgcgcga agccgacgat cgattcacga
     1681 cgcaattcgc ggaactcaag tttttgacct atcggccgac acggctcttt cagtcatagg
     1741 gggaatctcg atggttaccg atccgaaacc tcaatcgacc tccgccttag ctcgtccctg
     1801 gacgcaattg atcgtcggca tcatctgcat ggtgatggtg gcgaactgcc agtacggctg
     1861 gacgctgttc gtcggcccga tcggggacaa gtttcactgg accaaggcgg ccatccaaat
     1921 cacgttcacc ctcttcgtcc tcttcgagac ctggctggtt ccggtggagg gctacatcgt
     1981 cgatcggatc ggtccacgct ggatgatcgt gggcggcggc gcactcgccg gcctctcctg
     2041 ggtgctcaat tcctacgcgg actctctcgg gatgttctat ttcaacggaa tcgtcggtgg
     2101 catcggcgcg ggagccgtct acggcacctg catcggcaat gccgtcaagt ggttccccgg
     2161 gcggcgtg
//
LOCUS       DATLKL010000855         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_21618, whole genome shotgun sequence.
ACCESSION   DATLKL010000855 DATLKL010000000
VERSION     DATLKL010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21618"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VM713_11665"
     CDS             complement(<1..260)
                     /locus_tag="VM713_11665"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HVH84960.1"
                     /translation="MRKYPALALTSLLLVASCGGGGSSFTTPPPPPMSGPNVATLTVD
                     SGPAGTVNEAFVSVTICQPGSTSNCQTITGIQVDTGSSGLRI"
     gene            complement(282..746)
                     /locus_tag="VM713_11670"
     CDS             complement(282..746)
                     /locus_tag="VM713_11670"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HVH84961.1"
                     /translation="MRSFNIGSRVLIALALAGSASPALAGLGGDVSTIEADRASLKGA
                     LTSFSTVKGYGVHEITTPAGVHVREYVSSDGTVFALSWQGPVIPDLQQMLGAYYASYA
                     RAASTQHSANHRHLKVEQPGLVVESNGRMRAFYGRAWDPARLPPNFTAADIR"
     gene            993..1069
                     /locus_tag="VM713_11675"
     tRNA            993..1069
                     /locus_tag="VM713_11675"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1027..1029,aa:Asp,seq:gtc)
     gene            1101..>2061
                     /locus_tag="VM713_11680"
     CDS             1101..>2061
                     /locus_tag="VM713_11680"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HVH84962.1"
                     /translation="MLQRIGDALKGTEGSSKRKWITYLFVGALSIVFAAWGAYGIVNL
                     SFGGSNYAAEAGASKITLEQARNAWLREQAQWQQRLGGADIPAALRTRLQDQVLEGMI
                     SRALIAKRTHDLGYRVGHDELIEAVHNEPAFQISGQYSPDAARDALARAGMTPEAFQE
                     ELGNDLRRSQLENAIRGSDFLTPTEVARLRDLEDQEREVRYAILPADKFPAAPADDAA
                     IEAYYKAREAQYLTPESVHIQYGELRLDALEAQTTVSDADLHAQYDKDKSQLMTPEKR
                     RAQHILITATDDAAALKDAQDVLAQAKAGKDFGELARQYSKDPG"
BASE COUNT          372 a          672 c          696 g          321 t
ORIGIN      
        1 atgatgcgaa gtcccgagga gccggtgtcg acctggatgc cggtgatcgt ctggcagtta
       61 ctggtgctgc ccggctgaca gatcgtgacg ctgacgaagg cctcgttgac ggttcccgca
      121 ggtcccgagt ccacggtgag cgtcgcgacg ttgggaccgg acatcggtgg gggcggcggc
      181 gtagtaaagc tcgagccacc gccgccgcag gaggcgacga ggagcagcga agtcagggcg
      241 agggcggggt actttcgcat gccagttacc ttcgaggcgc gtcagcggat gtcggcggcg
      301 gtgaaattcg gcggcagccg ggccggatcc caggctctgc cgtagaaagc ccgcatgcgc
      361 ccgttcgatt ccacgacgag ccccggctgc tcgaccttga ggtgccggtg gttcgctgag
      421 tgctgcgtgg acgcggcccg ggcgtagctt gcgtagtaag cgccgagcat ctgctgcagg
      481 tccggaatga ccggcccctg ccagctcagg gcgaacaccg tgccgtctga agatacgtac
      541 tcgcgtacat ggacgcccgc cggggtcgtg atctcatgca ccccgtaacc cttcaccgta
      601 ctgaaggagg tgagtgcgcc cttgagcgag gcccggtcgg cctcgatcgt cgagacatca
      661 cccccaagcc cggcgagcgc gggggacgcg gagcccgcca gagcaagggc tatcagtacc
      721 cgggagccta tgttgaatga tctcactgcg accttcctaa agcggagtct cgcttccgat
      781 tgtgcccaga tgtgcgccgc gccgcccgct gtcggggcct ggcgacgcgc gtacccgcac
      841 cgcgggcgat gccgggtcga cccgtgcata gcgcgcggat gccgggtttg gttgaccatc
      901 gaggcgccgt agggttcagc gctcagaccc cgttccggcg aaagcgcttg tggccaagtt
      961 cctcaatccg ctaggattcg ccccccaaat tcggagcggt agttcagctg gttagaatac
     1021 cggcctgtca cgccgggggt cgcgggttcg agtcccgtcc gctccgccac ccgatccaat
     1081 agagataagc cagcccacac atgcttcagc gcatcggaga cgccctcaag ggcacggagg
     1141 gcagcagcaa gcgcaagtgg atcacgtacc tcttcgtcgg cgcgctctcg atcgtattcg
     1201 ccgcatgggg cgcctacggc atcgtgaacc tgagcttcgg cgggtctaac tacgccgcgg
     1261 aagccggcgc cagcaagatc accctcgagc aggcgcggaa cgcgtggctc cgtgagcagg
     1321 cgcagtggca gcagcgcttg gggggcgccg acatcccggc ggcgctgcgc acgcggctcc
     1381 aggaccaggt gctcgagggg atgatcagcc gggccctcat cgccaagcgc acccacgatc
     1441 tcggctaccg cgtgggccac gatgagctga tcgaagccgt ccacaacgag ccggcgttcc
     1501 agatcagcgg acaatattcc ccggacgccg cacgcgatgc gctcgcccgc gccggcatga
     1561 cgcccgaggc cttccaggag gagctgggga acgacctgcg ccgctcgcag ctcgagaatg
     1621 cgatccgcgg ttcggatttt ctcactccca ccgaggttgc ccgcttgagg gacctcgagg
     1681 accaggagcg cgaggtgcgc tacgcgattc tgcccgcgga caagttcccg gcagctcctg
     1741 ccgacgatgc ggcgatcgag gcgtactaca aggcacgcga ggcgcagtac ctcactcccg
     1801 agtccgttca cattcaatac ggagagctca ggctcgatgc gctcgaagcg cagacgacgg
     1861 tgagcgacgc ggatctgcat gcgcaatacg acaaggacaa gagccagctc atgacgcccg
     1921 agaagcgtcg cgcgcagcac attctcatca cggccacgga cgacgcggcg gcgctcaagg
     1981 acgcgcagga cgttcttgcg caggccaagg ccggcaagga ctttggtgaa ctcgccaggc
     2041 agtattccaa ggacccgggg t
//
LOCUS       DATLKL010000856         1622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_55532, whole genome shotgun sequence.
ACCESSION   DATLKL010000856 DATLKL010000000
VERSION     DATLKL010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55532"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VM713_11685"
     CDS             <1..390
                     /locus_tag="VM713_11685"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVH84963.1"
                     /translation="LLTRTAIGSQILAVAEDRQAAMLMGIRPDRMQALAWGISGASAG
                     IAGALIATFFPISPTVGESLALIAFVVVTLGGFGSVPGAMIAGLIIGLVESLSAYYCG
                     PVYQDAFVYALFVLVLWVRPHGLLGKA"
     gene            390..1367
                     /locus_tag="VM713_11690"
     CDS             390..1367
                     /locus_tag="VM713_11690"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVH84964.1"
                     /translation="MRWAWLLLGAAILGYPIVFADPFFQRLGALVLLSAISASAWNLV
                     GGYAGQVSVGHAVFFGAGAYMPLLFYKLWESSPLVGFPAGMVVSAIIAVLIGTPTFRL
                     SGHYFSMATIAVAELIRILVANWDFVGASIGLMGPAVGRGWWDLTFRSSVPYYYLFLA
                     LLAVLLGLTYAVQRSRIGYYLRAIRAGERAARSLGVPVSRYKLYALMLSAAFTSLAGS
                     LYAAMVGFADPTSAFGILVSVQMVLAAALGGAGRLFGPLLGAAILVPVQTATNSLFGG
                     SGTGLTYILYGGFIMLISRYEPGGLLELWERIRARRQAPAMAAEPRRAA"
     gene            1384..>1622
                     /locus_tag="VM713_11695"
     CDS             1384..>1622
                     /locus_tag="VM713_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVH84965.1"
                     /translation="MAFDGFHAVAGASLAVAEGEIVGLIGPNGAGKSTFFNCLAGDLT
                     PSAGRVFLDEAEVTRASPETHARLGIGRTFQVPAT"
BASE COUNT          191 a          572 c          533 g          326 t
ORIGIN      
        1 ttgctcacgc gcacagcgat cgggagccag atcctcgcgg tcgccgagga tcggcaggcg
       61 gccatgctaa tgggcatcag gcccgaccgc atgcaggcgc tcgcctgggg catctccggc
      121 gcgtcggccg gcattgccgg cgcgctcatc gccacattct ttcctatatc gcctaccgtc
      181 ggggagtccc tcgcgctcat cgcatttgtg gtggtgaccc tcggcggatt cggcagcgtg
      241 ccaggcgcga tgatcgccgg gctcatcatc gggttggtcg agtccctctc cgcatattat
      301 tgcggcccgg tctatcagga cgccttcgtc tatgccctgt tcgtgctcgt cctgtgggtc
      361 cggccgcacg gcctgttggg aaaggcctga tgcgctgggc ctggctcctc ctcggcgcgg
      421 cgatcctcgg ttacccgatc gttttcgccg atcccttctt ccagcgccta ggcgcgctgg
      481 tgctgctctc ggcgatctcg gcatcggcct ggaacctggt cggcggctat gccggccagg
      541 tctcggtcgg ccatgccgtg ttcttcggcg ccggtgccta catgccgctg ctattctaca
      601 agctgtggga gtcgagcccg ctggtcgggt tcccggccgg catggtcgtc agcgcgatca
      661 tcgccgtgct gatcgggacc cccacgttcc ggctctccgg ccactatttt agcatggcaa
      721 cgatcgcggt cgccgaactc atccgcatac tggtcgcgaa ttgggatttc gtcggcgcct
      781 cgatcggcct catgggcccc gcggtggggc gcggctggtg ggatctcacc tttcgcagct
      841 cggtccccta ctactacctc ttcctcgcgc tgcttgcggt cctcctcggc ctcacatatg
      901 cggtccagcg cagccgcatc ggctattacc tgcgcgccat ccgcgccggc gagcgtgctg
      961 cgcgcagcct gggcgtgccg gtgagccggt acaagcttta cgccttgatg ctgagcgccg
     1021 ccttcacctc gctcgcgggc tcgctctatg ccgccatggt cggcttcgcc gatcccacca
     1081 gcgcgttcgg catcctcgtc tcggtgcaga tggtgctggc ggcggcgctc ggcggcgcgg
     1141 gccggctatt cgggccgctt ctcggcgcgg ccatcctggt gcccgttcag accgccacga
     1201 acagcctgtt cggcgggagc ggcacgggcc tcacctacat tctctatggc ggattcatca
     1261 tgttgatcag ccgctacgag ccgggcggcc tgctcgagct gtgggagcgc atccgcgccc
     1321 ggcggcaggc gcccgccatg gcagcggaac cccgccgtgc tgcttagggc ggaccatgtg
     1381 acgatggcgt tcgacggctt ccacgccgtc gcgggcgcct cgcttgcggt ggcggaaggc
     1441 gagatcgtcg gcctcatcgg cccgaacggc gccggcaagt cgaccttctt caactgtctc
     1501 gccggcgacc tcacgccgag cgcgggacgg gtgttcctcg acgaggccga ggtgacgcgt
     1561 gcctcgcctg agacgcacgc ccggctcggc attggccgca cgttccaggt gccggcgacg
     1621 tt
//
LOCUS       DATLKL010000857         1713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_100883, whole genome shotgun sequence.
ACCESSION   DATLKL010000857 DATLKL010000000
VERSION     DATLKL010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100883"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VM713_11700"
     CDS             complement(<1..233)
                     /locus_tag="VM713_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVH84966.1"
                     /translation="MSLFWYLLWFVVAVGLLVTVHEFGHFYVARRLGFRVLRFSVGFG
                     KPLLKWTAGADATEYVIAAVPLGGYVKLLDEREG"
     gene            complement(230..1438)
                     /gene="dxr"
                     /locus_tag="VM713_11705"
     CDS             complement(230..1438)
                     /gene="dxr"
                     /locus_tag="VM713_11705"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HVH84967.1"
                     /translation="MIGVAVLGSTGSVGESTLDVLARHRERFSLVAVAAHRNAARLAG
                     QILEWQPAYAALADEDAARELVALLGGKAPRTRVLAGPGALEEIVGLGEVQCVMAAIV
                     GAAGLRSTLAAARAGKRLLLANKESMVMAGPLLLEAVRTAGATLLPIDSEHNAIFQCL
                     PAGARPGVAPPGLKRVLLTASGGPFRATPREALAAVTPEAACAHPNWVMGRKISVDSA
                     TLMNKGLEFIEACLLFGVTTSQVEILVHPQSIVHSLVEYADGSMLAQLSSPDMRTPIA
                     HALAWPERIDSGVVFLDLLKAGRLDFQAPDYDRFRCLALAQAAARAGGLHPVLLNAAN
                     EVAVQAFLERRLNFPGIAAVIEAVVERGAAGAIGALEDVLAADAEGRLRASERIVGIT
                     RSESVRGAHA"
     gene            complement(1435..>1713)
                     /locus_tag="VM713_11710"
     CDS             complement(1435..>1713)
                     /locus_tag="VM713_11710"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HVH84968.1"
                     /translation="GVLGGVALSALVAIGGSLWFHVSLAVSLPLCLATVAFSVVGDLT
                     EALLKRFAGMKDSGSLFPGHGGVMDRIDSLTGAAPVLLLGLTLLGVVA"
BASE COUNT          288 a          608 c          602 g          215 t
ORIGIN      
        1 ccctcgcgct cgtcgagcag cttcacgtag ccgccgagcg gtacggcggc gatcacgtac
       61 tcggtggcgt ccgcgcccgc ggtccacttg aggagcggct tgccgaagcc gacggagaag
      121 cgcagcaccc ggaagccgag gcggcgcgcg acatagaagt ggccgaactc gtgcaccgtg
      181 accagaaggc ccacggcgac gacgaaccag agtaagtacc agaacaggct catgcgtgag
      241 cgcccctcac cgactcactt cttgtaatgc cgacgatgcg ctccgaggcc cgcagtctcc
      301 cctcggcgtc ggcggccagc acatcctcga gagctccgat cgccccggcg gcaccccgtt
      361 cgaccaccgc ctcaatgacc gcggcgatgc cgggaaagtt caatcgccgc tcgagaaacg
      421 cctggacggc gacctcgttg gcggcgttca gcagcaccgg atgcagcccc ccggcgcgcg
      481 cggccgcctg ggcgagcgcg agacagcgga agcgatcgta gtcgggagcc tgaaagtcga
      541 gccggccggc cttgagcaga tcgaggaata ctacaccgga gtcgatgcgc tcgggccagg
      601 cgagcgcgtg tgcgatgggc gtgcgcatgt cgggggagga gagctgcgcg agcatcgagc
      661 cgtccgcgta ctccaccagc gagtgcacga tgctctgcgg atgcaccagg atctcgacct
      721 gcgaagttgt gaccccgaag agcaggcacg cctcgatgaa ctcgaggccc ttgttcatga
      781 gcgtggccga gtccaccgag atcttgcggc ccatcaccca gttggggtgg gcacacgccg
      841 cctcgggcgt caccgcggcg agcgcctcgc gcggtgtggc acggaacggg ccgccggagg
      901 ccgtgagcag tacgcgcttg aggcccggcg gcgcgacccc ggggcgcgcc ccggcgggca
      961 ggcactggaa gatcgcgttg tgctcgctgt cgatcggcag gagcgtggcg cctgcagtgc
     1021 gcaccgcctc gagcagcagc ggtcccgcca tcaccatcga ttccttgttg gcgagcagca
     1081 gccgcttgcc ggcgcgcgcc gcggcgagcg tggacctgag tcccgctgcg ccgacgatgg
     1141 ccgccatcac gcactgcacc tcgccgagcc ccacgatctc ctcgagcgcg ccggggcccg
     1201 cgagcacgcg ggtgcgcggc gccttgccgc cgagcagcgc tacgagctcg cgcgctgcat
     1261 cctcgtccgc gagcgcggcg taggcgggct gccactcgag gatctggccg gcgagcctcg
     1321 cggcgttacg gtgggcggcg acggcgacga gactgaagcg ctcgcggtgg cgcgccagca
     1381 cgtcgagcgt gctttccccg accgagccgg tggagccgag taccgcgacg ccgatcacgc
     1441 cacgactccg agcagcgtca atccgaggag cagcaccggc gccgccccgg tgaggctgtc
     1501 gatccggtcc atgaccccgc cgtgtccggg aaacagcgag ccggagtcct tcatgcccgc
     1561 aaagcgcttg agcagggcct cggtgagatc gccgacgacg gagaaggcca cggtcgccag
     1621 gcacagcggc agggacaccg cgagcgagac gtgaaaccac aggctcccgc cgatggcgac
     1681 gagggcactc agggccactc ctcccagcac tcc
//
LOCUS       DATLKL010000858         1649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_111980, whole genome shotgun sequence.
ACCESSION   DATLKL010000858 DATLKL010000000
VERSION     DATLKL010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111980"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VM713_11715"
     CDS             <1..974
                     /locus_tag="VM713_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84969.1"
                     /translation="TVRHCELWVRDEERGGDYTTIQLIYPDIVLEGIDTPKNLSGVPG
                     HHPFVKVEANLTPGYFWGRSMIADVQMLQDVLNKRLRDLKVMWDRNAAAPYSFSGFQS
                     ITEEQYYKLISEGGFISDPNPNAKASKLTEPPPGGYLEELEFLFKMFDEAGGFSPIMT
                     GQGEPSVRAGVHAQTLVRTSSPGLIDPATRIERQLADSGYLALRIMANNDPRIYITEG
                     GLEFLLAAFLDSHETFQVEVDSHSASPAFAEDSRQTAIALARAQAIGGEDLIEMLNPP
                     NAQLLLAHLKERKAAMAKAQQQQEAQALLTGQPPPGQGASRCQPQRH"
BASE COUNT          332 a          529 c          524 g          264 t
ORIGIN      
        1 cgaccgtgcg ccattgcgag ctatgggtgc gcgacgagga gcgcggcggc gattacacga
       61 ctatacagct aatctatccc gatattgtct tggagggcat cgatacccct aagaacttgt
      121 cgggcgtccc tggacaccat ccatttgtca aggttgaggc caatttgacc cccggttact
      181 tctggggccg gtcgatgatc gcggatgtcc agatgctcca agatgtcctc aataagcggc
      241 tgcgcgacct caaggtaatg tgggatcgca acgccgccgc gccgtacagc ttctcgggct
      301 ttcagtcgat caccgaggaa cagtattaca agctcatcag cgagggcggg ttcatttccg
      361 acccaaatcc gaacgcaaaa gcaagtaaat tgaccgagcc accgccgggc ggttacttgg
      421 aagagctaga gttcctgttc aaaatgtttg acgaggccgg cgggttttcg cccatcatga
      481 cgggccaggg ggaaccaagc gtgcgcgctg gcgtccatgc ccaaacgctg gtgcgcacgt
      541 cgtcgcccgg gctcatcgac cccgccaccc gcatcgagcg gcagctcgcc gacagcggct
      601 acctcgcgct gcgcatcatg gcgaataacg accccaggat ttacatcacc gagggcgggc
      661 ttgagttcct gctcgccgcg ttcttggact cgcacgagac gtttcaggtc gaggtcgaca
      721 gccacagcgc ctcgccggcc tttgccgagg acagccggca gaccgcgatc gcgctggcac
      781 gagcgcaagc gatcggcggg gaagacctca tagaaatgtt gaatccgccc aacgcgcagt
      841 tgttgctcgc gcatctcaag gagcgcaagg cggcgatggc gaaggcgcag caacagcagg
      901 aagcgcaggc cttgctcacc gggcaaccgc cgcccgggca gggcgcgagc cggtgccagc
      961 cgcagcggca ctgatgatcg acaaagaaac ttaccggcgc atttacagca gcgcttacca
     1021 agatggccgc cgagagggtg cggaaaatat gcgggagcgg gcggcgcgac tcgcggacgg
     1081 ctactgcacc gaccgcacag acgatgttgc gcgcggcatt cgcgctctac cgctcatagc
     1141 ggaggcgcta gactcctagg cctatgcagt attagctttc gcgctactag atttagccga
     1201 aagcgcgcag tcgtggcagc ggggatggat gccgacgggc cacctggccc cggcggcgca
     1261 ccagatgcag gcccaccagc agcccccggc ggccctccgg gagccggcgg tgcatcgcct
     1321 attctcgctg ctctggctcg cgcgcttggc gcgccgcaag cctcggctcc cggccagggc
     1381 gcacaggccg ccgcgctgca gcaagtaaaa atggctttgg atatgcttca atccgcgctt
     1441 cccagtttgg gaacggggag cgaagttcac cgcgacgtga tcaacgccgt cagccgcctc
     1501 tcgcgccaca tgccgcaggg catcccgacc gcgggggcgc aacagggcgc ggtgcaggat
     1561 ctgatgcgca acaccatccg caacgccatg ctgcaacgag tcatggcgaa ccagcaaggc
     1621 gggcaaggcg gcggacagca gccgatgcc
//
LOCUS       DATLKL010000859         2442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_157567, whole genome shotgun sequence.
ACCESSION   DATLKL010000859 DATLKL010000000
VERSION     DATLKL010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157567"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..>2442
                     /gene="cphA"
                     /locus_tag="VM713_11720"
     CDS             234..>2442
                     /gene="cphA"
                     /locus_tag="VM713_11720"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HVH84970.1"
                     /translation="MRILDRSVYVGPSLYAHFPVIRLELELDELEAWPTGRLGGAFVE
                     SLAAALPGLPEHGCSYREPGGFFRRMREGEGTWLGHVLEHVAIELQNIAGEAVTFGKT
                     RSAGPTGVYTVVYEYAQRDEGIAAGELGLRLLSSLLPEAIRPADAVPAGWSWPEARDQ
                     FIRFAQRRALGPSTMSLVRAAEARGIPWLRLNDQSLVQLGHGKFQQRIQATVTGRTPH
                     ISVELASDKEETNKILAGLGLPVPKQELVQSETAAVCAARRMGFPVVTKPYNGNHGRG
                     ISIRLHTDEEVAHGFNVAREHSRSVIVETFLEGDDHRLLVVNGELVAATRRTPGHVVG
                     DGEHTVEELIEIVNQDPRRGVGHEKVLTRLELDAQAHKMLERVQLTPKSVPAQGQIIY
                     LRSTANLSTGGTATDVTDVIHPDNREMAERAVRAIGLDVGGVDFLSQNITESYRKIGG
                     GICEVNAAPGFRMHVAPSEGTPRDVAAPVIEMLFPQGAPSRVPIAAITGTNGKTTTAR
                     MLAHIAKMAGYTPGLTTTDGVYIDGQRTVPGDMTGPVSARMVLADPQIDIAVLETARG
                     GLLRAGMGVNEVNVGAVINVQPDHLGLKGIDTLEQLAEVKRIVVEVATDCAVLNADDP
                     LVMKMSGYTEAKHICYVTMNPQHALVREHIRAGARACALEAGVNGQMITLYDRGGHIP
                     LLWTHLIPATLEGRAVHNVQNAMFAAAVAFSLGMKLDAIRQGLRTFDSTFFQAP"
BASE COUNT          398 a          846 c          825 g          373 t
ORIGIN      
        1 cgtcgatagt gccgaggtga gttactcttc cgtcgacagc gtcgaggaag gacagccggc
       61 ctgtgtgctg gggctgaagc tgcacgtgct ggtggccggc gccactttca acctgcatac
      121 gcgcgtcgcc agctgcggca cgttgagaaa gtcgaaggag tgacccgaga ctagagtccg
      181 acctcgcgcg gtttggttgc cttgaacaac tattacgacc ctgagcaaca cccatgcgca
      241 tcctcgatcg ttcggtgtac gtcggcccgt cgctgtacgc gcatttcccc gtcatccgcc
      301 tcgagctcga gctggacgag ctcgaggcct ggcccaccgg ccgcttaggt ggcgccttcg
      361 tcgagtcgct cgccgcggcg cttcccgggc tccccgagca cggctgctcg taccgcgagc
      421 ccggcggctt cttccgccgc atgcgcgagg gcgagggtac ctggctcggc cacgtgctcg
      481 agcacgtcgc gatcgagctg cagaacatcg ccggcgaggc agtcaccttc ggcaagacgc
      541 gcagcgccgg ccccaccggc gtctacaccg tggtctacga gtacgcgcag cgcgacgagg
      601 gcatcgccgc cggcgagctc gggctgcggc tgctctcctc gctgctgccg gaggcgatcc
      661 gcccggccga tgcggtgccg gcgggctgga gctggcctga agcccgggac cagttcatcc
      721 gcttcgcgca gcggcgcgcc ctcggtccct cgaccatgtc gctggtgcgc gcggcggagg
      781 cgcgcggtat tccctggctg cgcctcaacg accagtcgct cgtgcagctc ggccacggca
      841 agttccagca gcgcatccag gcgaccgtca ccggtcgcac gccgcacatc tcggtcgagc
      901 tcgcgagtga caaggaggag accaacaaga tcctcgcggg cctcgggctg ccggtgccga
      961 agcaggagct ggtgcagagc gagaccgcgg cggtgtgtgc cgcgcgccgc atgggtttcc
     1021 cggtggtcac caagccctat aacggcaacc acggacgcgg catctccata cgcctgcaca
     1081 ccgacgagga agtggcccat ggcttcaacg tcgcgcgcga gcactcgcgc tcggtcatcg
     1141 tcgagacctt cctcgagggg gacgaccatc ggctgctggt cgtcaacggc gagctggtgg
     1201 ccgcgacgcg ccgtacgccc gggcacgtgg tgggcgatgg cgagcacacc gtcgaggagc
     1261 tcatcgagat cgtgaaccag gacccgcgcc gcggggtggg gcacgagaaa gtgctcacca
     1321 ggctcgagct cgacgcgcag gcgcacaaga tgctcgagcg cgtgcagttg acgcccaagt
     1381 cggttcccgc gcagggccag atcatctacc tgcgctcgac ggcgaacctc tccaccggcg
     1441 gtaccgccac cgacgtcacc gatgtgatcc accccgacaa ccgcgagatg gccgagcgcg
     1501 ccgtgcgcgc catcgggctc gacgtcggcg gcgtcgattt tctctcccag aacatcaccg
     1561 agagctaccg caagatcggc ggcggcatct gtgaggtgaa cgctgcacct ggcttccgca
     1621 tgcacgtggc gccgagcgag ggcacgccgc gcgacgtcgc cgcgccggtg atcgagatgc
     1681 tgtttccaca gggggcgccg tcgcgcgtgc cgatcgccgc gatcaccggg acgaacggca
     1741 agacgaccac cgcacgcatg ctggcccaca tcgccaagat ggccggttac accccggggc
     1801 tcaccaccac cgacggcgtg tacatcgacg gccagcgcac cgtaccgggt gacatgaccg
     1861 ggccggtctc ggcgcgcatg gtactggccg atccgcagat cgacatcgcg gtgctcgaga
     1921 ccgcgcgcgg cgggttgctg cgtgcgggca tgggcgtcaa tgaggtcaac gtcggggcgg
     1981 tgatcaacgt gcagccggat cacctggggc tcaagggcat cgatacgctc gagcagctcg
     2041 cggaggtcaa gcgcatcgtc gtcgaggtgg cgaccgactg cgcggtgctg aatgccgatg
     2101 acccgctggt gatgaagatg tcgggttaca cggaagcgaa gcacatctgc tacgtcacga
     2161 tgaacccgca gcatgccctg gtgcgcgagc acatccgcgc cggcgcgcgc gcctgcgcgc
     2221 tcgaggccgg tgtcaacggc cagatgatca ctctctacga ccgcggtggg cacatcccgc
     2281 tcctctggac ccacctgatc cccgcgactc tcgaaggccg cgccgtccac aacgtgcaga
     2341 acgcgatgtt cgcggcggcc gtggcgttct cgctcggcat gaagctggat gcgattcgcc
     2401 agggcctgcg caccttcgac tcgacgttct tccaggcgcc gg
//
LOCUS       DATLKL010000860         1601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_112000, whole genome shotgun sequence.
ACCESSION   DATLKL010000860 DATLKL010000000
VERSION     DATLKL010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112000"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VM713_11725"
     CDS             <1..667
                     /locus_tag="VM713_11725"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVH84971.1"
                     /translation="ETVTYMLDGRMRHRDNHGNEGVLVPGSVQWMTAGRGLVHSEMPE
                     QQEGRMRGFQLWLNLPAREKMTAPKYQEFGPERIPTASPAAGVTAKVIAGKVAGVVGP
                     IAQPATDPTYLDLAIAPEATFREPLPAEYAAFLYVYEGTISVGEGADAITVRTHELAV
                     LDEGTEVQLTGVTAGADGKSARAILVAGRPLREPVAKYGPFVMNTRDELQQAFIDFQN
                     GKF"
     gene            complement(639..1517)
                     /gene="modA"
                     /locus_tag="VM713_11730"
     CDS             complement(639..1517)
                     /gene="modA"
                     /locus_tag="VM713_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HVH84972.1"
                     /translation="MSRSVRATAAAVAALCAASALLAAAFGVAANPPAAKGAEAENTA
                     PAIIVFAAASLAEPVGEIARAFTARAAEPGERASARVPVRTSFAASSVLAKQIEAGAP
                     ADVFLSADTEWMAYLEERHLLREGSQQNLLGNQLVLIAPAGSTVEVTLRPHANLAAAL
                     GGGRLATGDPDLVPVGRYALAALMNLGMWDQVAPRLVRAENVRMALEYVARGEATLGI
                     VYRTDALAEKRVRLVDVFPASSHPPVIYPVALTAGAAPAAAAFEAFLESDAAREVFVR
                     YGFERLPLRTSRSESR"
BASE COUNT          264 a          574 c          541 g          222 t
ORIGIN      
        1 cgagaccgtc acctacatgc tcgatggccg catgcgccac cgcgacaacc acggcaatga
       61 gggcgtattg gtcccgggga gcgtgcagtg gatgacggcg ggccggggac tcgtgcactc
      121 ggagatgccc gagcagcagg agggccgcat gcgcggcttt cagctgtggc tcaacctccc
      181 ggcgcgcgag aagatgaccg cgcctaagta ccaggagttc ggccccgagc gcattccaac
      241 cgcctcaccc gccgcggggg tcacggccaa agtgatcgcc ggaaaggtgg caggtgtcgt
      301 gggtcccatc gcgcagcccg cgaccgaccc gacttacctc gatctcgcga tcgcgccgga
      361 ggcgacgttc cgcgagccgc tgcctgctga gtacgcggcg ttcctctacg tctacgaggg
      421 aacgatcagc gtgggcgagg gggcggatgc catcacggtc cgcacgcacg agctcgcagt
      481 gcttgatgag gggaccgagg tgcagctcac tggtgtgacc gccggcgccg atggcaagag
      541 tgcccgcgcg atcctggtcg ccggccggcc gctgcgcgag ccggtggcga agtacggtcc
      601 cttcgtgatg aatacccgcg acgagctgca gcaggccttc atcgactttc agaacgggaa
      661 gttctgagcg gcaagcgctc gaacccgtag cgcacgaaga cctcccgggc cgcatcgctc
      721 tcgaggaagg cctcgaaggc cgcggccgcg ggcgccgctc ccgcagtcag cgccaccggg
      781 tagatgacgg gcggatgact cgaggcggga aagacgtcga cgaggcgcac gcgcttctcc
      841 gcgagcgcat ccgtccggta gacgatccct aaagttgcct ccccgcgcgc cacatactcg
      901 agcgccatgc gtacgttctc ggcgcgcacg agacgcggcg ccacctgatc ccacatcccg
      961 aggttcatga gcgccgcgag cgcgtagcgg ccgacaggca ccaagtcggg atctcccgtg
     1021 gcgagccgcc cgcccccgag cgcggcggcg aggttcgcgt gcggccgcag agtcacctcc
     1081 accgtgctcc cggccggcgc gattagcacg agctgattcc cgaggaggtt ctgctgcgac
     1141 ccctcgcgca gcagatgccg ctcctcgaga tacgccatcc actccgtgtc cgctgacaga
     1201 aacacgtccg caggtgcgcc ggcctcgatc tgcttggcga gcaccgagct cgccgcgaag
     1261 gaggtccgca cgggaacgcg tgcgctcgcg cgctcgcccg gctccgcggc gcgcgcggtg
     1321 aatgcgcgcg cgatctcgcc cacgggctcg gcgagcgacg cggctgcaaa cacgatgatg
     1381 gcgggcgcgg tgttttccgc ctcagcgccc ttcgccgcgg gcgggttcgc cgccacaccg
     1441 aaagccgccg ccagcaacgc actcgccgcg cagagcgccg cgactgcagc ggcggtcgcc
     1501 cgtaccgatc gactcatcga tcccctcccc cggaacgcgc cgcaagatcg gcagtgagtc
     1561 acattttggc gacgctccgt tcgaactgct acctgcgcac c
//
LOCUS       DATLKL010000861         2768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_224375, whole genome shotgun sequence.
ACCESSION   DATLKL010000861 DATLKL010000000
VERSION     DATLKL010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224375"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1748
                     /locus_tag="VM713_11735"
     CDS             120..1748
                     /locus_tag="VM713_11735"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HVH84973.1"
                     /translation="MSETLGIALAQINLLVGDVQGNAARVVAAARSARVELGADLVLL
                     PELALSGYPPEDLLFHRGFRRQIEAGLARVRSEAGAAAVMMGFPEYTRAGIYNSAALV
                     DGGEVAAIHRKAELPNYKVFDEKRYFRAGAQPTVVELKAFGVGLLVCEDIWEREAAQL
                     ARAAGAQLLAVINASPYEIHKQREREDIARARVTDVGVPLAYVNLVGGQDELVFDGNS
                     FVMSAAGEVVLRAPPFEEGCYLARFERGARGEVVPRAAEVAPELSDEASVYRALTLGV
                     RDYVGKHGFPGVVMGLSGGVDSGLTLAIAVDALGAERVQAVMMPSRYTSAMSVEDAAE
                     QARNLGVRYSVLPIEAMFESTLATLKGEFTGREPDATEENIQSRCRMLLLMGISNKTG
                     RMLLTTGNKSEMAVGYATLYGDMAGGFAPIKDCSKGLVYRLANYRNSLSRVIPLRVID
                     RPPSAELRPGQKDSDSLPPYEVLDPILEAFIEEDLSVDEIEARGFDRATVARVLDLVK
                     RNEYKRRQAPPGVRVSRRAFGRDWRYPITSGYRR"
     gene            complement(1773..2573)
                     /locus_tag="VM713_11740"
     CDS             complement(1773..2573)
                     /locus_tag="VM713_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HVH84974.1"
                     /translation="MLLSGPVRGGALAFCVVLLAVGGCGSHREKALQKQTPEQIFKTA
                     KKELANNNFSGAIKSYESLTARFPFTDEARQARIDLIYAYYRAGENESATDAADAFIR
                     ENPTHPRVDYAYYLKGLVDFERTPNAIERLFRADLTQRPPTNARKAFASFKIVVEQYP
                     KSEYAHDALQRMVYLRDRLASYEVHVARYYLKRGAYVAAAQRARGAVEQYDGAPAVRD
                     ALAIMIQCYDKLGLEPLAAQSREVYAANFSADVRQSEAVAKRSWWKFW"
BASE COUNT          438 a          920 c          972 g          438 t
ORIGIN      
        1 aaaagacggg gcgcggcgcc gcgacacaag gcgccggcgc gcgcgacgac gcgcgggcgt
       61 ccgcgccccg ccgcccgccg gccgggaggc agccggcggc gctgaggcgg taaggcgcca
      121 tgagcgaaac cctcggcatt gcgctcgcgc agatcaacct cctcgttggc gacgtccagg
      181 gcaacgccgc acgcgtggtc gcggcggcac gcagcgcccg cgtggagctc ggcgccgacc
      241 tggtgctctt gcccgagctc gccctgagcg gctatccgcc cgaggacctg ctgtttcacc
      301 gtggttttcg ccgccagatc gaggcggggc ttgcgcgcgt gcgctccgag gctggcgcgg
      361 cggcggtgat gatgggcttt ccggaataca cccgcgccgg aatctacaac tccgcggcac
      421 tcgtcgacgg cggcgaggtc gccgccatcc atcgcaaggc ggagctgccg aattacaagg
      481 tcttcgacga gaagcgctac ttccgcgccg gggctcagcc gacggtggtc gagctcaagg
      541 cctttggggt cgggctcctg gtctgcgagg acatctggga gcgggaagca gcacagctgg
      601 cgcgcgccgc cggcgcgcag ctgctcgccg tgatcaacgc ctcgccctat gaaatccaca
      661 agcagcggga gcgcgaggac atcgcccggg ctcgtgtcac cgatgtgggc gtgccgctcg
      721 cctacgtgaa cctcgtcggc ggccaggacg agctggtgtt cgacggcaac tcgttcgtca
      781 tgagcgccgc gggcgaagtg gtgctgcggg cgccgccctt cgaggagggc tgctatctcg
      841 cgcgcttcga gcgcggcgcc cgcggcgagg tggtgccgcg cgctgccgag gtggcgcccg
      901 agctcagcga cgaggcgagc gtgtaccgcg cgctcacgct cggggtgcgc gactacgtcg
      961 gcaagcacgg gtttccgggg gtcgtgatgg gcctctcggg aggagtcgat tcgggcctga
     1021 cgctcgcgat cgccgtggac gcactcggag ccgagcgcgt gcaggcggtc atgatgccct
     1081 cgcgctatac ctcggcgatg agcgtggagg acgcggcgga gcaggcgcgg aacctcggag
     1141 tccgctacag cgtgctgccg atcgaggcga tgttcgaaag cacgctggcg acactcaagg
     1201 gcgagttcac cggccgcgag cccgatgcca ccgaggagaa catccaatcc cgctgtcgca
     1261 tgttgcttct catgggcatc tccaacaaga ccggtcggat gctgctgacc acgggcaaca
     1321 agagcgagat ggcggtcggg tacgccacgc tctacggcga catggccggc ggcttcgcgc
     1381 ccatcaagga ctgcagcaag gggctggtat atcgcctggc gaactaccgc aactcgctct
     1441 cgcgggtgat tccgctgcgc gtgatcgacc ggccgccctc ggcggagctg cgtccgggac
     1501 agaaggactc cgactcgctg ccgccctatg aggtgctcga cccgatcctc gaggcattca
     1561 tcgaggaaga cctgtcggtg gatgagatcg aggcgcgcgg gttcgatcga gccaccgtcg
     1621 cgcgcgtgct cgatctcgtg aagcgcaacg agtacaagcg ccgccaggca cctcccggcg
     1681 tgcgggtgag ccgccgcgcc ttcggtcgcg actggcgcta tccgatcacg agcggctacc
     1741 ggcgctaaga ggcgcgtcgc ccccgggacc tgctaccaga atttccacca ggagcgtttc
     1801 gcgacggcct cggactgacg gacgtccgca gagaagttcg cggcatatac ttcgcgcgac
     1861 tgcgcggcga gcggctcgag ccccagcttg tcgtagcact ggatcatgat cgcgagcgcg
     1921 tcgcggaccg ccggcgcccc gtcatactgc tccaccgcgc cccgcgcgcg ctgcgcggcc
     1981 gccacgtaag cgccgcgctt caggtagtaa cgcgccacat gcacttcgta gctggcgagg
     2041 cgatcgcgca ggtacaccat gcgctgcagc gcatcgtggg cgtactcgct cttcgggtac
     2101 tgctcgacca cgatcttgaa ggaggcaaag gccttgcggg cattggtcgg cggccgctgg
     2161 gtcaagtcgg cgcgaaacag cctttcgatc gcattcggcg tgcgctcgaa atcgaccagc
     2221 cccttcaggt aataggcata gtcgacgcgc gggtgcgtcg ggttctcgcg aatgaaggca
     2281 tcggcggcgt cggtggccga ctcgttctcg ccggcgcggt aatatgcata gatgagatcg
     2341 atgcgcgcct ggcgcgcctc gtcggtgaag ggaaagcgcg cggtgagcga ctcataggac
     2401 ttgatggcgc cgctgaagtt gttgttggcg agttccttct tcgccgtctt gaagatctgc
     2461 tccggcgtct gcttctgcag cgccttctcg cggtgcgagc cgcagccgcc gacggcgagc
     2521 agcactacgc aaaacgcgag cgcgccgccg cgtaccggac cggacagaag catctggaag
     2581 gggcccttct ttggctttgc gccgaacgaa tcgagtaagt atagcgaatc tgacggctgg
     2641 gcttccgacc gccccgcccc cggcagagtt ccgctcgcat gagctcgagc tgcccgcgga
     2701 ggccgccggc ctcaggttcg atcaggcgtt ggcacgtgcg ctgccgcagt actcgcgcgc
     2761 gcaactca
//
LOCUS       DATLKL010000862         1869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_224377, whole genome shotgun sequence.
ACCESSION   DATLKL010000862 DATLKL010000000
VERSION     DATLKL010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224377"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..842
                     /locus_tag="VM713_11745"
     CDS             63..842
                     /locus_tag="VM713_11745"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HVH84975.1"
                     /translation="MRRTRNRRKPTARSLRVSGIAWRRLGAVLGALAALGAAVAALAT
                     ALDQPVGSVSVEGSFQRVSPVDVERAVKSEVHGVGLLSVDLTAVRRAIHTLPWVDAVS
                     VQRAWPRGLNVLVIEQTAAARWGDEGLINTRGELFAADPRHIPPELAQLSGPPDQQAV
                     VAQRYLAAAGRLTAAGLHLTALRLDARGAWEFDLSNGVTVRLGRRQVDERFEKFMSSA
                     LKLVTERGEEISYVDMRYTNGFAIGWRGSARAAGREGGRNA"
     gene            835..1869
                     /gene="ftsA"
                     /locus_tag="VM713_11750"
                     /pseudo
     CDS             835..1869
                     /gene="ftsA"
                     /locus_tag="VM713_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020030439.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
BASE COUNT          290 a          654 c          653 g          272 t
ORIGIN      
        1 gacttcgacg agctcgcctg gcgcgtgctc gagaccagcc tcgcccggca cgcggccccg
       61 ccatgagacg cacccgcaac cgccgcaaac cgaccgcgcg ttccttgcgg gtgagcggta
      121 tcgcctggcg gcgcctcggc gcggtgctcg gcgcgctcgc cgccctcggc gccgcggtcg
      181 ccgccctcgc gacggcgctc gatcaaccgg tcggctcggt gtcggtcgag gggagctttc
      241 agcgcgtctc gccggtcgac gtcgagcgcg ccgtcaagag cgaggtgcac ggtgtcgggc
      301 tcctgtcggt ggatctcacg gcggtgcggc gcgcgatcca tacccttccc tgggtggacg
      361 ccgtgagcgt gcagcgcgcc tggccgcgtg gcctcaacgt gctggtcatc gagcagaccg
      421 cggccgcccg ctggggcgat gaaggcctca tcaacacccg cggcgagctc ttcgcggcgg
      481 acccgcgcca cattccaccc gagctcgccc agctctccgg tccccctgac cagcaggcgg
      541 tagtcgcgca gcgctatctc gccgccgccg ggcgcctcac ggcggcgggg ctgcacctga
      601 ccgcgctgcg gctcgatgcg cgtggcgcct gggagttcga tctctccaac ggagtcaccg
      661 tgcgcttagg tcgccgccag gtggacgagc gcttcgagaa gttcatgagc agcgcactga
      721 agctcgtcac cgagcgcggc gaggaaatct cctacgtcga catgcgttac acaaacgggt
      781 ttgccatcgg ctggcgcggc agcgcgcgcg ccgctggcag ggaaggtggc cgcaatgcgt
      841 aagcgctccg aacgcaacct gatcgtcggt ctcgacatcg gcacctccaa ggtcgtgtcg
      901 ctggtcgccg aggcgggcgc cgacggctcg atcgagctgc tcggcatcgg ctcgcagccc
      961 tcgcgcggac tcaagaaggg cgtggtcgtc aacatcgagt cgaccgtgca gtcgatccag
     1021 cgcgccgtcg aggaggccga gctcatggcc ggctgcgaga tccactcggt gttcgccggc
     1081 atcgccggca gtcacgtgcg cagcctcaac tcgcacggcg tggtcggggt gcgcgacaag
     1141 gaggtgacgc acggcgacgt cgaccacgtg atcgatgccg ccaaggcggt cgccattccc
     1201 gccgaccaga agatcctgca cgtgctgccg caggaattca tcgtcgacgg ccaggaaggc
     1261 atccgcgacc cgatcggcat gtcgggagtg cggctcgagg ccaaggtgca catcgtcacc
     1321 ggggccgact cggccgcgca gaacatcgag aaatgcatcc agcgctgcgg gctcgacgtc
     1381 gacgacgtgg tgctcgagca gctcgcctcg agcttcgccg tgctcaccga ggatgagaag
     1441 gagctcgggg tgtgcctggt cgacatcggc ggcggcacca ccgatatcgc ggtgttcgcc
     1501 aacggcgcca tccgtcatac ggcggtgatc cccatcgccg gcgaccaggt caccaacgac
     1561 atcgcggtgt cgatgcgcac gccgacgcaa tacgccgagg acatcaagat ccgctacgcc
     1621 tgcgcgctct cgcagctcgc caatcccgac gagagcatcg aggtgccgag cgtcggcgag
     1681 cgccccgccc ggcgtctggc ccggcagacc ctggccgaga tcgtcgagcc gcgctacgag
     1741 gagctcttcg gcctggtgcg cgaggagctg cggcgcagcg gcttcgagga ggtgatcgcc
     1801 gcgggaatcg tgctcaccgg ggcagcgcca agatggaagg cgcgatcgag ctagccgagg
     1861 aggtgttcc
//
LOCUS       DATLKL010000863         3153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_134765, whole genome shotgun sequence.
ACCESSION   DATLKL010000863 DATLKL010000000
VERSION     DATLKL010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134765"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2869)
                     /locus_tag="VM713_11755"
     CDS             complement(<1..2869)
                     /locus_tag="VM713_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVH84976.1"
                     /translation="MALLLPSSLSWTLLLYAALVLATAWAYAAQRIRTDREQTYQSES
                     NRLRAVAVALEQSAFAMISDGVGSAIIRAHEVEAAGGLNAVSERDLSPALGQAMSGGQ
                     YVRSVFVADATRFARAGRAGSFDVSRAPPPWFNAIASRGADVWIGVPIPDPDRPPGEA
                     HDNLLVPIARHVPSTTNTNLWAGALFDFRAFESLRTQLGGPGGVIFIVTRDGTLLAAM
                     EDKPAHGVIGRNYSSSPAFAAAKRYLASGWDSGTVEAYAPVYGTRMLFGFAQLPQYAA
                     LIATARPLDSILAPWHERTVTTLVVTGAFSALVLAMTFLLSHSLRALRNREVHYRTLF
                     NNASYSVFLGEGDRFIAANDTALRMFGLADQEALRTLTAEQMSPLEQPDGRPSGLARR
                     QRIEQAMRDGNASFEWLHKRLDTGATFPAEVDLSILREDKSLLTLAVVHDLTETKRAE
                     QERRESEGRYRALVDAMPEAVFVHRGKELLFGNAAARVLIGAKPDDPLAGISVMSFAL
                     ESDRKVLAERTRRILEHGHAAEPRPARIRRLDGSIIWVEVQGVRIEYEGAPAVQTVMR
                     DITARKEQEEAEAARIVRMQRQSDMLLRVASHGASTGWSGLRSALEGICVDTSQAMGA
                     DRVTLWLVEDEGATLSCTAFYERGSGLREGELPRLPTSRALHLPALRNERVIDGAALF
                     DVDLRLRELREATPTVSNPSRALLVAAVRRSGELIGTLTIGQLESARLWHPDEVNFAG
                     AVADQVAQVLLDFERERVLADLQVLAGELTRIQNEERRRIGRELHDSTGQTLAALELD
                     LARLSEATKSLAAKPRELLDSAVRLAQQCSTEIRTASYLLHPPLLDELGLVSALRWLA
                     DGLRERSGLDVHLDLPESMPRLSGANELTLFRVAQEALTNVQRHSASPWVALRLAVQT
                     NSVTLEVEDAGRGIARDHLTRGAQGVPTIGVGLAGMRERIRQ"
BASE COUNT          487 a         1095 c         1095 g          476 t
ORIGIN      
        1 cctggcggat gcgctcgcgc atgcccgcga gccccacccc gatcgtcggc acgccctgcg
       61 cgccgcgcgt gaggtggtcc ctggcgatgc cgcgcccggc gtcctcgact tccagagtca
      121 ctgagttcgt ctgtaccgcg aggcgcagcg ccacccacgg gctcgccgag tgacgctgca
      181 cgttggtgag cgcctcctgt gccacgcgga agagcgtgag ctcattggcg cccgacagac
      241 gcggcatgga ttcggggagg tcgagatgca cgtcgaggcc gctgcgctcc cgcaggccgt
      301 cggcgagcca gcgcagtgcg ctcacgagtc cgagctcatc gaggagcggc gggtgcagca
      361 ggtaggaggc ggtgcggatc tcggtggagc actgctgcgc gaggcgcacg gcgctgtcga
      421 gcagctcgcg tggcttggcg gcgagcgatt tggtcgcttc ggagaggcgc gccaggtcga
      481 gctcgagcgc ggcgagcgtc tggccggtgg agtcgtgcag ctcgcggccg atgcggcgtc
      541 gctcctcgtt ctggatgcgc gtcagctcgc cggcgagcac ctgcaggtcc gcgagcaccc
      601 gctcgcgctc gaaatctagc agcacctgcg ccacctggtc ggcaactgca ccggcgaagt
      661 tcacctcgtc cgggtgccag aggcgcgcac tctcgagctg cccgatcgtc agagtaccga
      721 tgagctcgcc cgagcggcgc accgccgcga ccaacagggc gcgcgaagga ttgctgacgg
      781 tgggcgtcgc ttcccgcaac tcccgcaggc gcaggtcaac gtcgaagaga gcagcgccgt
      841 cgatcacgcg ctcgttgcgc agcgccggga gatgcagcgc gcggctcgtc ggcagcctcg
      901 gcagctcgcc ctcgcgcagc ccgctgcccc gctcgtaaaa cgcggtgcaa ctcaaggtcg
      961 cgccctcgtc ctcgaccagc cagagcgtca cccggtcggc ccccatggcc tgggaggtgt
     1021 cgacgcaaat gccctcgagg gccgaccgca atccgctcca gccggtgctg gcaccatggc
     1081 tcgccacccg taacagcatg tccgactggc gttgcatgcg gacgatgcgt gccgcctcgg
     1141 cctcctcctg ttccttgcgg gcggtgatgt cccgcatgac ggtctgcacg gcgggcgcgc
     1201 cctcgtactc gatccgtacg ccctgcacct ccacccagat gatgctgcca tcgagccggc
     1261 ggatgcgcgc cgggcgcggc tccgcggcgt ggccgtgctc gaggatccgc cgggtgcgct
     1321 ctgcgagcac cttgcggtcg gactcgagcg caaaggacat gaccgagatg ccggccagcg
     1381 ggtcatcggg cttcgcgccg atcagtaccc gcgcggctgc gttaccgaac agcagctcct
     1441 tgccgcggtg cacgaacacc gcctcgggca tcgcatccac cagcgcccgg tagcggccct
     1501 cgctctcgcg ccgctcctgc tcggcgcgct tcgtctcggt gaggtcgtgc acgaccgcga
     1561 gggtcagcag gctcttgtcc tcgcggagga tgctgagatc cacttcagcc gggaacgtcg
     1621 cgccggtgtc gaggcgcttg tgcagccact cgaagctggc attcccgtcg cgcatcgcct
     1681 gctcgatgcg ctggcgccgc gcgaggcccg agggccgccc gtccggctgc tcgagcggcg
     1741 acatctgctc ggccgtgaga gtccgcagcg cttcctggtc cgccagcccg aacatgcgca
     1801 gcgcggtgtc gttggcggcg atgaagcgat caccttctcc gaggaacacg ctgtaggagg
     1861 cgttgttgaa gagtgtccgg tagtgcacct cgcgattgcg cagggcacgc agcgagtgac
     1921 tgaggaggaa ggtcatggcg agcaccagcg ccgagaacgc gccggtcacc accagcgtcg
     1981 tcaccgtacg ctcgtgccag ggagcgagga tgctgtcgag cggccgggct gtggcgatga
     2041 gggcggcgta ctgcggcaat tgcgcgaacc cgaagagcat gcgcgtgccg tataccggcg
     2101 catacgcctc gaccgtcccc gagtcccagc cgctcgccag ataacgcttg gccgccgcga
     2161 acgcgggcga ggaggaatag ttgcggccga tcactccgtg cgcgggctta tcctccatgg
     2221 cggcgagcag cgtgccatcc ctcgtgacta tgaagatgac ccccccaggc cccccgagtt
     2281 gggtgcgcag ggattcaaag gcgcggaagt cgaacagggc gcccgcccag aggttcgtgt
     2341 tagtggtcga gggcacgtgg cgcgcgatcg gcaccagcag gttgtcgtgc gcctcgccgg
     2401 gcgggcgatc ggggtcgggg atcggtacgc caatccaaac gtccgcaccg cgggatgcga
     2461 tcgcgttgaa ccagggcggc ggcgcccggc tcacatcgaa cgaccccgcc cgtccggcac
     2521 gcgcgaagcg cgtcgcgtcg gcgacgaaca ctgagcgcac gtactgcccg ccgctcatcg
     2581 cctggccgag agccgggctc aggtcgcgct ccgagaccgc attcagaccg ccggccgcct
     2641 cgacctcgtg ggcgcggatg attgccgatc ccacaccgtc gctgatcatc gcgaacgcgc
     2701 tctgctcgag cgccacggcg actgcacgca ggcgattgct ctccgactgg taggtctgct
     2761 cgcggtcggt gcggatgcgc tgtgccgcat aggcccaggc ggttgcgagc acgagcgcgg
     2821 cgtacagcag cagcgtccag ctcaggctgg acggaagtaa cagcgccacg tccccgacac
     2881 tcgatccagg tcgggagttc acagggacct ctcgttcccc gggcaggcgt accccctgat
     2941 ccattcacag ggccgcggca gttattgtgg ccatcagttt gctctaaagc gccgccggag
     3001 gccatgcccc gtgagtcaac cagggaacgc cccgccccgt taacggggca gacgggctcg
     3061 aacacgcaag gttaaataga gtctaatgga acgaccggcg ccggtacgcc ggaaagggcg
     3121 ccctatgtgg attcagagtc tcaggctggc gcg
//
LOCUS       DATLKL010000864         1514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_123437, whole genome shotgun sequence.
ACCESSION   DATLKL010000864 DATLKL010000000
VERSION     DATLKL010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123437"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..>1514
                     /locus_tag="VM713_11760"
     CDS             24..>1514
                     /locus_tag="VM713_11760"
                     /inference="COORDINATES: protein motif:HMM:NF015592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84977.1"
                     /translation="MDFSASPPGAPQAWLNRARLDDRHFAGYRQTLDLHEATLTTRYR
                     YLDGRRATEVEVTTLVSEALPHLAATRLTITPDYDGIVHLSFPLLLWADHAPRFPLAQ
                     MTGPEMEEAVAAYGLTLEPRPPATADREAVWYPGHTEVRASDGDASTVSMWLDGRAAQ
                     GLAMAMAAAVELPEGTAPESVSFHRDAYRMALDVAIWVQHGGSYTFTKYVALSREGWG
                     GDAAQDLALAREARGRGFERLLGEHRAAWAQLWQSDILVEGDPRAQQVTHSELYYLLA
                     SSTAGTAFSPGPDALTPDYAAHVFWDSDTWIFPVLLLLHPERAQPIVDFRYRTLEPAR
                     QRAQAHGYQGAMYPWESDPENGTDQTPHSAVLLADTEIHVTAEVAIAQWQYYLATRDR
                     EWLRTHGWPVIRDVARFWASRSTYNAREHRYEITHVNSVAESHNDIPNDTFTNVSAAR
                     ALAIAAAAARELGERPDPVWEKAAREMYVPLAAGGTHHLPFDPSVVR"
BASE COUNT          228 a          537 c          526 g          223 t
ORIGIN      
        1 cgcagctacc ggcgtggacc gaggtggact tcagcgcaag tcccccgggc gcgcctcagg
       61 cgtggctcaa ccgcgcacgg ctcgacgatc gccatttcgc cggctaccgg cagacgcttg
      121 acctgcatga ggccacgctg accacgcgct atcggtacct cgacggccgc cgtgcgaccg
      181 aggtcgaggt gacgacgctc gtgagtgagg ccctgccgca cctcgcggcc acgcgcctca
      241 ctatcacgcc ggactacgac ggcatcgtcc atctgtcgtt tccgctgctc ctgtgggcgg
      301 atcacgcccc gcggttcccg ctcgcgcaga tgacggggcc ggagatggag gaggcggtcg
      361 ccgcgtacgg cctgacgctc gagccgcgtc ccccggccac ggccgaccgc gaggcagtct
      421 ggtatccggg acacaccgag gtgcgcgcga gcgacggcga cgcgtcaacg gtctcgatgt
      481 ggctcgacgg cagggccgcg cagggcctcg ccatggccat ggccgcggcg gtcgagttgc
      541 cggaggggac cgcgcccgag agcgttagct ttcatcgcga tgcctatcgc atggcactcg
      601 acgtggcgat ctgggtccag cacggtggca gctatacctt caccaagtat gtggcgctgt
      661 cgcgggaggg ctgggggggc gacgccgcgc aggatctcgc gctcgcgcgc gaagcgcgcg
      721 gccgtgggtt cgaacggctc ctcggcgagc atcgcgccgc ctgggcgcag ctctggcagt
      781 cggacatcct ggtcgagggt gatccacgcg cgcagcaggt gacgcactcg gagctctact
      841 acctgctcgc cagctcgacc gccggcacgg cgttctcgcc gggtcccgat gcgctcacgc
      901 cggactatgc ggcccacgta ttctgggaca gcgacacctg gatcttcccc gtactcctgc
      961 tgctgcaccc cgagcgcgcg cagccgatcg ttgatttccg ctaccgtacg ctcgagccgg
     1021 cgcggcagcg ggcgcaggca cacggctacc agggggcgat gtacccctgg gagtcggatc
     1081 ctgagaacgg caccgatcag acgccgcact ctgccgtgct gctcgcagac accgagatcc
     1141 acgtgaccgc cgaggtcgcg atcgcgcagt ggcagtacta cctcgccacc cgcgatcgcg
     1201 aatggctgcg cacgcacggc tggccggtga tccgcgacgt ggcgcgcttc tgggcgagcc
     1261 gctcgacgta caacgcgcgc gagcaccgtt acgagatcac gcacgtgaac tcggtggccg
     1321 agtcgcacaa cgacattccg aacgacacct tcaccaacgt atcggccgcg cgagcgctcg
     1381 cgatcgcggc ggcggcggct cgggagctcg gcgagcggcc cgatccggtg tgggagaagg
     1441 cagcgcggga gatgtacgtg ccgctcgccg ccggcggcac gcatcacctg ccgttcgatc
     1501 cgtcggtcgt gcgc
//
LOCUS       DATLKL010000865         1568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_100963, whole genome shotgun sequence.
ACCESSION   DATLKL010000865 DATLKL010000000
VERSION     DATLKL010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100963"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..736
                     /locus_tag="VM713_11765"
     CDS             74..736
                     /locus_tag="VM713_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH84978.1"
                     /translation="MRLLLVEDDPMIGEAIRAGLRREGFTIDWVTDGGSAGQALDTEP
                     FELLLLDLGLPGGDGLQLLKTLRGRGSALPVLIITARDAVADRVAGLDAGADDYLVKP
                     FALEELAARIRALLRRKSGRPEPRLEHLGVALDPATHRVTRDGADVALSPREFALLEL
                     LLERPGAILSRGQLEERLYGWGEEVESNAVEVHIHGLRRKLGAQFILTVRGVGYRVRP
                     AP"
     gene            733..>1568
                     /locus_tag="VM713_11770"
     CDS             733..>1568
                     /locus_tag="VM713_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVH84979.1"
                     /translation="MSSLRARLLAWLLSGVVLVGAAGGWIVYRNALAEADAFFDYHLR
                     QTALILRDQHVEYLLPQQIAPADAAYDFVVQVWSLDGVRVYLSRPHAVLPEMTTLGLS
                     TVSTSEGRWRVYGVQALTRVIQVAQPMSVREQRAAQLALRTLAPFALLLPVLALLIWV
                     AVGQALRPLERLTGLVKARQVNALEPLPDTQLPDEVRPLVGALNELLARLGAALGRER
                     AFMADAAHELRTPLTALHLQMDTLARASGEAERGAAMEQLSEGMQRAIRLVEQMLSLA
                     RE"
BASE COUNT          201 a          563 c          548 g          256 t
ORIGIN      
        1 cgctcggtcg ggctcaactt agctcgctcc tatgaacgat ccggggcgcg attgatcata
       61 ctcgcggaca gtcatgcgac ttctcctggt agaagacgac cccatgatcg gcgaggccat
      121 ccgcgcgggc ctgcggcgcg agggctttac gatcgactgg gtgaccgacg gcggctccgc
      181 cggccaggcg ctcgacaccg agcccttcga gctgctgctc ctcgacctgg ggctcccggg
      241 gggcgatggt ctccagctcc tcaagacact gcgcgggcgc ggctcggcgc tgccggtcct
      301 catcatcacc gcgcgcgatg cggtggcgga ccgggtggcc gggctcgatg ccggcgccga
      361 tgattatctg gtcaagccct tcgctctcga agagctggcg gcgcgcatcc gcgcgctgtt
      421 gcgccgcaag tcggggcgcc ccgagccgcg cctcgagcac ctcggcgtgg cactcgatcc
      481 ggccacgcat cgcgtgaccc gcgacggagc cgacgtggcg ctctcgccgc gcgagttcgc
      541 gctcctcgag ctgctgctcg agcgacccgg cgcaatcctc tcgcgcggcc agctcgagga
      601 gcggctctat ggctggggcg aggaagtgga gagcaacgcc gtcgaagtcc acatccacgg
      661 cctgcggcgc aagctcggcg cgcagttcat cctcacggtg cgcggagtcg gctatcgcgt
      721 gaggccggcg ccatgagctc gctgcgcgcg cggctgcttg cctggctcct cagcggggtc
      781 gtgctggtcg gtgccgccgg tggctggatc gtgtatcgca acgcgctcgc cgaggcggac
      841 gcattcttcg actaccacct gcgtcagacg gccctcatcc tgcgtgatca gcacgtcgag
      901 tacctcctgc ctcagcagat cgcgcctgcg gatgcggcct acgatttcgt cgtgcaggtc
      961 tggtcactcg atggggtgcg cgtgtacctg tcgcgcccgc acgcggtgct gcccgagatg
     1021 accacgctcg gcttgtcgac cgtgagcact tcggagggac gctggcgcgt ttacggcgta
     1081 caggcgctca cccgcgtgat ccaggtcgcc cagccgatga gcgtgcggga gcagcgcgcg
     1141 gcgcagcttg cgcttcgcac gctcgcgccc ttcgcgctgc tcctgccggt gctcgcgctc
     1201 ctgatctggg tcgcggtcgg gcaggcgctc cggcccctcg agcggctcac cggcctcgtc
     1261 aaggctcgcc aggtgaacgc gctcgagccg ctccccgata cgcagctccc cgacgaggtg
     1321 cgcccgctgg tcggcgcact caatgagctg ctcgcgcgcc tgggtgccgc gctcggacgc
     1381 gagcgcgcct tcatggccga tgccgcgcac gagctgcgca ctcccctcac ggcgctgcat
     1441 ctgcagatgg acacgctggc gcgggcgagc ggcgaggccg agcgcggcgc cgccatggag
     1501 cagctgtccg agggtatgca gcgcgccatc cgcctggtcg agcagatgct ctcgctcgcg
     1561 cgcgagga
//
LOCUS       DATLKL010000866         1648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_157667, whole genome shotgun sequence.
ACCESSION   DATLKL010000866 DATLKL010000000
VERSION     DATLKL010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157667"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..>1648)
                     /locus_tag="VM713_11775"
     CDS             complement(85..>1648)
                     /locus_tag="VM713_11775"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HVH84980.1"
                     /translation="VWYPRASALGGCTSHNAMIFMLPHDSDWQHIAALTQDPSWSPRN
                     MRRHVQRVEACHYRPLWRVLRHFGVDPTGHGWDGWLSTELPMELETLGDPEMRALVAG
                     TAAAFARTRPLGQILRWLRSAADPNSHSWGKRSFEGLCYTPLSTAHHARTGAGERVLR
                     AAVALRGALHIEPHALATRVLLDESQGAVGVEYLKGQHLYRADPAAGHSAGELRQVHA
                     RREVILAGGAFNTPQLLMLSGIGPAAQLREHGIAVRVDLAGVGSNLQDRYEVAVTHRM
                     RRSWRVLKGALFEQGDRLWERWAAQRDGLYASNGAAIAFVNRSAPEAPEPDIFGMALP
                     TRFEGYFPGYSRYLQRDHDYLTWALLKAHTHNRAGTVALRSADPRDTPQINFHYFEEG
                     TDRQQLDLRGLVRAIGLVRDLTAPLIMKGVIEKECAPGPGVTTAAALEQYIRDTAWGH
                     HACGSCPIGPTHKGGVLDSKFAVHGTRRLRVVDASVFPRIPGFFLVSAVYIIAEKAAQ
                     TILGSTVTGSRE"
BASE COUNT          254 a          542 c          586 g          266 t
ORIGIN      
        1 cttggcctgg gacatcgcca gtaattgatc tacgtcgtat gccatgtttg tctccttcgc
       61 ggccgcaggc cgcgatctca ggcgtcactc ccgggatcct gttacggtgc tcccgagtat
      121 ggtttgtgcg gctttttccg cgatgatgta aacggcgctc accaggaaga aacccggaat
      181 gcgcgggaat accgaggcat cgaccacccg cagccggcgc gtcccgtgga cggcgaactt
      241 gctgtcgagc acaccgccct tgtgagtggg gccgatcggg caggagccgc aggcatggtg
      301 gccccaggcc gtgtcgcgga tgtattgctc gagagccgcg gcggtcgtta ccccgggtcc
      361 gggggcacac tccttctcga tcacgccctt cattattaag ggcgcggtga ggtcgcggac
      421 gaggcctatg gcccgcacca gcccgcgcag gtcgagctgc tgcctgtcgg tgccttcctc
      481 gaagtagtga aagttgatct gcggagtgtc gcgcgggtcg gcagagcgca gcgcgacagt
      541 ccccgcgcga ttgtgcgtgt gggccttgag cagcgcccag gtgagataat cgtggtcgcg
      601 ctgcaggtag cgcgaatagc cgggaaagta gccctcgaag cgcgtcggca gtgccatgcc
      661 gaagatgtcc ggctcaggtg cttcaggcgc ggagcggttc acgaaggcga tcgcggcgcc
      721 gtttgaggcg tagaggccgt cacgctgcgc ggcccagcgc tcccagagcc gatcgccctg
      781 ctcgaacagg gcgcccttga gcacgcgcca ggaacgtcgc atgcggtgcg tgaccgccac
      841 ttcatagcga tcctgcagat tgctgccgac gcccgccaga tcgaccctga ccgcaatgcc
      901 gtgctcgcgc agctgcgctg cggggccgat gccggagagc atcaggagct ggggagtgtt
      961 gaaagctcca ccggcgagga tgacttcgcg gcgcgcgtgc acctggcgca gctccccggc
     1021 gctatggccg gccgccgggt ccgcccggta gagatgctgg cccttgaggt actcgacgcc
     1081 caccgcgccc tgcgactcat cgaggagcac gcgcgtcgcg agtgcgtgag gctcgatatg
     1141 cagcgcgcca cggagcgcga ccgcggcgcg cagcacgcgc tcgccggccc cggtgcgggc
     1201 gtgatgcgcc gtggagagcg gcgtgtagca aaggccttcg aagctccgct ttccccagct
     1261 gtgcgagttg ggatcggcgg cgctgcgcag ccagcgcagg atctgcccga gcggccgcgt
     1321 gcgcgcgaac gccgctgctg tcccggcgac cagcgcgcgc atctcggggt ccccgagcgt
     1381 ctccaactcc atcggcagct cggtgctcag ccagccgtcc cagccgtgtc cggttggatc
     1441 gacgccgaag tgccgcagca cgcgccacag cggccggtag tggcacgcct cgacgcgctg
     1501 gacgtgccgg cgcatgttgc gcgggctcca ggacgggtcc tgggtgagcg cggcgatgtg
     1561 ctgccagtcc gagtcgtggg gcagcatgaa gatcatcgca ttgtgcgagg tacagccgcc
     1621 gagggcagac gcgcgcggat accacacg
//
LOCUS       DATLKL010000867         1560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_33201, whole genome shotgun sequence.
ACCESSION   DATLKL010000867 DATLKL010000000
VERSION     DATLKL010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33201"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..515
                     /locus_tag="VM713_11780"
     CDS             90..515
                     /locus_tag="VM713_11780"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVH84981.1"
                     /translation="MSASGTHNPPEFKHAEDMEWEMGRFGNVTKFLFHPRPERPTEPN
                     AGFLEYAPGASFPLHRHDFAQVWCIIEGEFTFAGKLYGPGTIAYMPDPHFEHEMHTAT
                     GGTIFFVQYPGPNTGAPPIYDGRMNLKEAPPLAQQDLEH"
     gene            563..>1560
                     /locus_tag="VM713_11785"
     CDS             563..>1560
                     /locus_tag="VM713_11785"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HVH84982.1"
                     /translation="MTWQIGVDVGGTFTDLLALDPEHGAFRVAKVLSTPEDQSVGFIA
                     GLSLLETDLAAVAALVHGTAVATNAVLERKGARCGLITTAGFRDVLELGRRTRPNPYG
                     MTGSFEALIPRDLRAEVPERIDAAGRILAPLDEAAVRQEVHRLRERGAEALVIHFIHA
                     YANPEHEQRCAEIVREIWPNQFVTLGSNILREVREFERGSTAALNGYVQPIVSRYLGR
                     LSQNLRSGGLSNELPVMQGNGGMMAASTAVDLAVHTVLSGPAAGAIAAARIGVQAGYP
                     NLVACDMGGTSFDVSLIAAGEPALSAEKDIAYGVPLRVPLVDIHTIGAGGGSIARIT"
BASE COUNT          278 a          515 c          515 g          252 t
ORIGIN      
        1 caagacgcgc ttcgcgtgac cggcgtgcat cgtcttggtg accctgcccg ttcaacccgc
       61 gatcgggtgc aaaaacggga gaacgcgaca tgagtgcgag cggcacgcat aacccgcccg
      121 aattcaagca cgccgaagac atggaatggg agatggggcg atttggcaat gtcaccaaat
      181 tcctctttca cccgcgaccg gaacgtccga cagagccgaa tgcgggattt ctcgaatatg
      241 cgccgggcgc gtctttcccg ctgcaccggc acgatttcgc gcaggtctgg tgcatcatcg
      301 agggcgaatt cacgtttgcc ggaaagctct acggccccgg cacgatagcc tatatgcccg
      361 acccgcattt cgagcatgaa atgcacacgg caaccggcgg gaccatcttc ttcgtacaat
      421 atccgggtcc taacaccggc gcgccgccga tctatgacgg ccgcatgaac ctgaaagagg
      481 cgccgccgct ggcgcagcag gacctggagc actgatgcgc gtgcgcaacc gagaaccgcg
      541 gcaagccgcc gaggaatagc ggatgacgtg gcagatcggg gtcgatgtcg gtggcacgtt
      601 caccgacctg ctggcgctcg atccggagca cggcgcattc cgcgtcgcca aggtgctttc
      661 aacccccgag gaccagtcgg tcggcttcat cgccgggctt tccctgctcg aaaccgatct
      721 ggccgcggtg gccgcgctgg tgcacggcac cgcggtcgcc accaatgccg tgctcgagcg
      781 caagggggcg cgctgcgggc tgatcaccac ggccgggttt cgcgacgtgc tcgagctcgg
      841 ccgccgcact cgccccaatc cctatggcat gaccgggagc ttcgaggcat tgatcccgcg
      901 cgacctgcgg gccgaggtgc ccgagcgcat cgatgccgcc ggccggattc tggcgccgct
      961 cgacgaggca gcggtgcgcc aggaagtgca tcgcttacgc gagcgcggcg ccgaggcgtt
     1021 ggtgatccat ttcatccatg cttatgccaa cccggagcat gagcagcgct gcgccgagat
     1081 cgtgcgggaa atctggccca accagtttgt gacgttgggc agcaacatct tgcgcgaggt
     1141 gcgtgaattc gaacgcggca gcaccgcggc gctcaacggc tacgtgcagc cgatcgtgtc
     1201 gcgctatctc ggccgcctct cacagaacct gcgcagcggg gggctcagca acgagcttcc
     1261 cgtgatgcag gggaatggcg gcatgatggc ggccagcacc gccgtcgatc ttgccgtgca
     1321 caccgtcctg tccggcccgg ccgccggagc gatcgccgcg gcgcggatcg gcgtgcaggc
     1381 cggctatccc aacctcgtcg cctgcgacat gggcgggacc agcttcgatg tctctctgat
     1441 cgcagcgggc gaaccggcgc tgagcgcgga gaaggacatc gcctacggtg tgccgttgcg
     1501 cgtgccgctg gtcgacatcc acacgatagg ggccggcggc ggcagcatcg cgaggatcac
//
LOCUS       DATLKL010000868         2307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_21767, whole genome shotgun sequence.
ACCESSION   DATLKL010000868 DATLKL010000000
VERSION     DATLKL010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21767"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VM713_11790"
     CDS             <1..862
                     /locus_tag="VM713_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVH84983.1"
                     /translation="EGAVRLADLLERLGARATFLFSLGPDHTGRAIKRVFRRGFLGKV
                     KRTSVVEHYGVKTLLYGVLLPGPHIGRRCRSFMQHTAKSGFEVGVHTWDHIRWQDGVA
                     RASEPWTRRELALARQEFTEIFGRAPEVHGAAGWQMNRFVPALEQQLGFRYASDTRGT
                     GPFVPLVDGSAVAVPQLPTTLPTFDELIGREDLGGADPVDHLLALTDRAGGGDQVFTL
                     HAELEGGAYLDSFERLLRAWKARGARLTDLAGFARELDPKALPRCSIIAGSVAGRSGT
                     LAVQASGPGA"
     gene            936..1481
                     /locus_tag="VM713_11795"
     CDS             936..1481
                     /locus_tag="VM713_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVH84984.1"
                     /translation="MSRDRQIAAAVVRERGRLLAFIRRRIDDAAEAEDILQDALYELV
                     AAERLAQPVEQIGAWLMRVARNRIIDRFRKKRPELLPQGTGGADGEGGLEELLPAADG
                     TPETAAMRALLLEEIEAALAELPREQREVFIAQELDGVTFRELAARTGVSINTLLSRK
                     RYAVLQLRERLQAVYEDWLTD"
     gene            1498..1869
                     /locus_tag="VM713_11800"
     CDS             1498..1869
                     /locus_tag="VM713_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84985.1"
                     /translation="MSKAGWVARRVAKAVAIAVAVVMVAVVFSWVVMLLWNYVVPPVF
                     HGPVLGYWQAFALLVLCRMLFGGLRGWRGSHGHWRRHAWRERWESMTPEERERLRERF
                     RERCGRGMDAPAADEHAQPGS"
     gene            1917..>2307
                     /locus_tag="VM713_11805"
     CDS             1917..>2307
                     /locus_tag="VM713_11805"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVH84986.1"
                     /translation="MTAIEIREPGPPQVLRPARRPVPKPRPGEVLIRVTAAGVNRPDV
                     LQRKGVYPPPPGASDIPGLEVAGVVVAAAEGVSEPAVGARVCALLAGGGYAEYATAPA
                     VQCLPAPSQLTLEEAAALPETFFTVWYN"
BASE COUNT          324 a          782 c          861 g          340 t
ORIGIN      
        1 cgagggcgcc gtgcggctcg cggaccttct cgagcggctc ggtgcgcgcg cgaccttcct
       61 cttcagctta gggcccgacc acacaggccg tgccatcaag cgcgtattcc ggcgcggctt
      121 cctcggcaag gtgaagcgca cctcggtggt cgagcactac ggcgtgaaga cgctgctcta
      181 tggcgtgctg ctccccgggc cgcacatcgg gcgccgctgc cgcagcttca tgcagcacac
      241 ggctaagagc ggcttcgagg tgggtgtgca cacctgggac cacattcgct ggcaggacgg
      301 cgtggcccgc gcgagcgagc cgtggacgcg tcgcgagctc gcgctcgccc gccaggagtt
      361 caccgagatc ttcggacggg cgccggaggt gcacggcgca gccggctggc agatgaaccg
      421 cttcgttccc gccctcgagc agcagctggg gttccgctac gcctcggaca cccgcggcac
      481 gggccccttc gtgccgctcg tcgacgggag cgccgtcgcc gtgccgcagc taccgaccac
      541 gctgccgacc ttcgatgagc tgatcggccg cgaggatctc ggcggcgccg atccggtcga
      601 ccatctgctg gcgctcaccg accgtgcggg tgggggcgac caggtgttta ccctgcacgc
      661 cgagctcgag ggcggcgcct acctcgattc cttcgagcgg ctgctgcgtg cctggaaggc
      721 gcgcggagcg cgcctcacgg atctcgcagg attcgcccgc gagctggacc cgaaggcgct
      781 gccgcgttgc agcatcatcg ccggcagcgt cgccggccgc tcgggcacgc tcgccgtcca
      841 ggcgtcggga cccggagcct gacgccgcca caatatcggc accccgccga ccgtctctcc
      901 tggtggagat ggacgccgcc gcgatcagcg accccatgag ccgggatcgg caaatcgccg
      961 ccgccgtggt gcgcgagcgc gggcggctgc tggccttcat tcgccggcgc atcgacgatg
     1021 cggccgaggc ggaggacatc ctgcaggacg cgctctacga gctggtcgcg gccgagcgcc
     1081 tggcgcaacc cgtggagcag atcggcgcct ggctgatgcg ggtcgcgcgc aaccggatca
     1141 tcgatcgttt ccgcaagaag cgaccggaat tgctcccgca gggaaccggc ggcgccgacg
     1201 gggagggagg gctcgaggag ctgctgcctg ccgcggacgg aacgccggag accgcggcga
     1261 tgcgcgcgtt gctcctcgag gagatcgagg cggcgctcgc ggagctgccg cgcgagcagc
     1321 gcgaggtgtt catcgcgcag gagctcgacg gcgtcacctt ccgtgagctc gccgcgcgca
     1381 cgggcgtgag catcaacacg ctgctatcgc gtaagcgcta cgcggtgctg cagctgcgcg
     1441 agcggctgca ggcggtatac gaggactggc tgaccgattg agtaagagga gaaagtcgtg
     1501 agtaaggcag gttgggtggc gaggagagtg gccaaggcag tcgcgatcgc ggtcgccgtg
     1561 gtcatggtcg cggtagtctt cagctgggtg gtgatgctgc tgtggaacta tgtggtgccg
     1621 cccgtgtttc acggcccggt gctcggctac tggcaggcgt tcgccctctt ggtgctgtgc
     1681 cgcatgctct tcggcggcct gcgcggctgg cgcgggtcgc acgggcactg gcggcggcac
     1741 gcgtggcgcg agcgctggga aagcatgaca cctgaggaac gggagcgcct gcgcgagcgg
     1801 ttccgcgagc gctgcgggcg gggcatggat gcgccggcgg cggacgagca cgctcagccc
     1861 ggaagttgac gcgcgaatgg gagcgcccgc accatccgcg cgtgctccca gtcaacatga
     1921 ccgccatcga gatccgcgag cccggcccgc cgcaggtgct gcgtccggcg cgacgtccgg
     1981 tgccgaagcc caggcccggc gaagtgttga tccgggttac ggccgcaggc gtcaatcggc
     2041 ccgacgtgct gcagcgcaag ggcgtctatc cgccaccgcc gggcgccagc gatattccgg
     2101 gcctcgaggt cgccggcgtt gtagtggcgg cggcggaggg agtgagtgag ccggcggtcg
     2161 gcgcccgggt gtgcgccctg ctcgccggcg gcggctacgc ggaatatgcg accgcgccgg
     2221 ccgtgcagtg cctgccggcg ccgtcccagc tcaccctgga ggaggcggcc gcgctccccg
     2281 aaacgttctt taccgtctgg tacaacg
//
LOCUS       DATLKL010000869         1529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_44513, whole genome shotgun sequence.
ACCESSION   DATLKL010000869 DATLKL010000000
VERSION     DATLKL010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44513"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1529
                     /locus_tag="VM713_11810"
     CDS             <1..>1529
                     /locus_tag="VM713_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84987.1"
                     /translation="FDWSYDVLSEPERAALRRLGIFMGGFTLDAACAVLTDPGLLKGD
                     VIDAVAELVEKSLAVVEATETEPRLRLLETTRAYALERLGEGYEREAVARRHTEYYRN
                     LFERAEGEAAARPADEWLADYAREIDNLRAALDWAFSLGGDGSIGVALTAAAVALWMR
                     LSLPEECGGRAEQALAALGAGAGQDARHEMKLRAAVAASASPETSMAYTRALEIAESL
                     DDAEYRLRALCGLWAYHTASGRHRVALELAQRFYALAAERPDRNDRLSGEGMIGISQH
                     YLGDLPSARRHLEHVLAGYVTPDQTSHIIRFRTDQRVWMSVFLARILWLQGFPDQATR
                     TAHSSVDDARAANHTMSLCQALAHSACLALLTGDLAAAEHYGTMLLDHSTRHALARWR
                     ASGRSFQGALVIQRGDVITGLRLVRTHFDEFGEAGSALLPFFALLMAEALGHAGQNAD
                     GLAAIVEAIARSERTEQRWAMAELMRVRGELLLLQGAPGATLAAEHHFRQALDCARRQ
                     GA"
BASE COUNT          254 a          489 c          513 g          273 t
ORIGIN      
        1 ttcgactgga gctacgacgt gctctccgag cccgagcgtg ccgcgctgcg ccgcttgggg
       61 atcttcatgg gcggcttcac tctcgatgca gcctgcgcag tcctcaccga tcctggcctt
      121 ctcaagggcg acgtcatcga cgccgtcgcg gaactggtcg aaaaatcgct cgccgtcgtg
      181 gaagcgaccg aaaccgagcc gcgcctgcgg ctgctcgaaa ccacccgcgc ctatgctctt
      241 gaaaggctcg gcgaaggcta cgagcgagaa gcggtcgcgc gccgccacac cgagtactac
      301 cgtaatcttt tcgagcgcgc tgagggtgaa gcggcggcgc ggcctgcgga cgaatggctg
      361 gccgattatg ctcgggaaat cgacaatttg cgcgccgcgc tcgactgggc cttttcgctg
      421 ggcggcgatg gatcgatcgg ggtagcgctg accgccgctg cggtcgccct ctggatgcgc
      481 ttgtcgctgc cggaggagtg cggcggccgc gccgagcaag cgctcgccgc tctcggggcc
      541 ggagcgggcc aggatgcgcg ccacgaaatg aagctccgcg ccgcagtggc tgcatctgcc
      601 agccccgaga ccagcatggc ctacacaagg gcccttgaga ttgcggagag ccttgacgac
      661 gccgagtatc ggttgcgggc gctgtgcggc ctgtgggctt atcacaccgc cagcggtcgg
      721 catcgcgttg ctctcgaact agcgcaaagg ttctatgcgt tggccgcgga gcgacccgac
      781 cggaacgatc ggctgagcgg cgagggaatg atcggcatct cgcagcatta cctcggcgac
      841 ctgcccagcg cgaggcgcca tctggaacac gtgctcgccg gttacgtcac tcccgaccaa
      901 acgtcgcaca ttattcgctt tcggaccgat caacgggtgt ggatgagtgt ttttcttgcg
      961 cggattctat ggctgcaggg atttccggat caggcgacgc gcacggccca cagcagcgtg
     1021 gacgacgctc gcgcagctaa ccacacaatg tcactatgtc aggctctggc ccactcggcg
     1081 tgcctcgctc tgttgaccgg tgatctggct gcggcggaac attacgggac gatgctgctc
     1141 gaccattcga caaggcatgc actggcccgt tggcgtgcca gtggccgcag ctttcaggga
     1201 gcgctcgtca tccagcgcgg cgatgtcata accggattgc gactggtgcg aacccacttc
     1261 gacgaattcg gcgaggccgg gtccgccctc ttgccgttct tcgccttgct gatggcagaa
     1321 gctttgggcc acgctgggca gaatgccgac gggctcgccg cgatcgtgga ggcgatcgcg
     1381 cgttccgagc gcaccgagca acgctgggca atggccgaat taatgcgcgt caggggcgag
     1441 cttctgctgt tgcagggcgc gcccggagcc acgttggcgg ccgagcatca cttccggcaa
     1501 gcgctcgact gcgcgcgcag gcaaggggc
//
LOCUS       DATLKL010000870         2984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_168690, whole genome shotgun sequence.
ACCESSION   DATLKL010000870 DATLKL010000000
VERSION     DATLKL010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168690"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /gene="glyA"
                     /locus_tag="VM713_11815"
     CDS             complement(<1..1049)
                     /gene="glyA"
                     /locus_tag="VM713_11815"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HVH84988.1"
                     /translation="MFRSNETIADFDPELARALEGERRRQEDHVELIASENYASPRVL
                     AAQGSVLTNKYAEGYPGKRYYGGCEFVDVAEQLAIDRAKQLFGADYANVQPHSGSQAN
                     AAAYFALVQPGDPILGMSLDHGGHLTHGAKVNFSGKFFRAVQYGIRPENGEIDYAQVQ
                     RLAEEHRPKMIVAGFSAYSRVVDWARFATIARAVGAYFVVDMAHVAGLVAAGIYPNPL
                     PHADVVTTTTHKTLRGPRGGLILARANDEITKKLNSMVFPGIQGGPLMHVIAAKAVAL
                     KEALQPEFRDYQRQVLANARAMAARLAKNGYQIVSGGTDNHLFLMSLADRNITGKDAD
                     AALGRANITVNKNAVP"
     gene            complement(1171..2109)
                     /locus_tag="VM713_11820"
     CDS             complement(1171..2109)
                     /locus_tag="VM713_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HVH84989.1"
                     /translation="MNAGVGVVVSEQLARYSFGDGHPFGPDRLAAFMREYEARGLDRR
                     VQVLEPRTATEEELRAFHSPAYLEFVRERSVSGTGLLDAGDTPAFRGVYEAAAGVVGA
                     TLAAMEAIMRGDCRRAFVPIAGLHHAARERAAGFCVFNDCGVAIELLKRAGLRRVAYV
                     DIDAHHGDGVFYAFEDDAAVVFADLHEDGRYLYPGTGASGETGRGAAAGMKLNVPLPP
                     GADDSVFEQVWPRVLAHLERFEPEFLILQCGADSLEGDPITHLRLSARSHGHAARSLK
                     ELADNLGHGRVLALVGGGYNRGNLAQAWNAVLENLL"
     gene            2230..>2984
                     /locus_tag="VM713_11825"
     CDS             2230..>2984
                     /locus_tag="VM713_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVH84990.1"
                     /translation="MAQYIYTMNRVSKVVPPKRVILRDISLSFFPGAKIGVLGLNGAG
                     KSSLLRIMAGTDQEFDGEARPQSGIRIGFLPQEPQLDASRDVRTTVMEGLGETFRQLE
                     EFNHISERFAQELSEEEMAKLLERQAQLQDAIDAAGGWELERKLEIAADALRLPPWEA
                     SVATLSGGERRRVALCRLLLSAPDMLLLDEPTNHLDAESIAWLERYLEEYPSTVIAVT
                     HDRYFLDNAAQWILELDRGYGIPWEGNYSSWRE"
BASE COUNT          458 a         1016 c         1040 g          470 t
ORIGIN      
        1 ggcacggcgt ttttgttgac cgtgatgttg gcgcggccca acgccgcgtc ggcgtccttg
       61 ccggtgatgt tgcggtcagc gaggctcatg aggaacaggt ggttgtcggt gccgccggag
      121 acgatctgat agccgttctt cgcgagtcgc gccgccatcg cccgcgcgtt ggcgagcacc
      181 tggcgctgat agtcgcggaa ctccggctgc agcgcctcct tgagcgcgac tgctttggcc
      241 gcgatgacgt gcatcagggg ccccccctgg attccgggga acaccatcga attgagcttc
      301 ttggtgatct cgtcgttggc gcgcgccagg atcagtccgc cgcgcgggcc gcgcagcgtc
      361 ttgtgggtgg tggtggtcac gacgtcggcg tgcggcagcg ggttgggata gatcccggcc
      421 gcgaccagcc cggcgacgtg cgccatgtcg accacgaagt acgcgcccac cgcgcgcgcg
      481 atggtggcga agcgcgccca gtcgacgacg cgcgagtagg cggagaagcc ggcgacgatc
      541 atctttggcc ggtgctcctc ggcgaggcgc tgcacctggg cgtagtcgat ctcgccgttc
      601 tcggggcgaa taccgtactg cacggcgcgg aagaatttgc cggagaagtt gaccttggcg
      661 ccgtgtgtca ggtgcccgcc gtggtcgagg ctcatgccga ggatcggatc gccgggctgc
      721 accagcgcga aataagcggc ggcgttcgcc tgcgagcccg agtgcggctg cacgttggcg
      781 tagtcggcgc cgaagagctg cttcgcgcgg tcgatggcca gctgctcggc gacgtcgacg
      841 aactcgcagc cgccgtagta acgcttgccg gggtagcctt cggcgtactt gttggtgagc
      901 accgagccct gcgcggcgag cacgcgcggg ctcgcgtagt tctccgaggc gatcagctcg
      961 acgtgatcct cctggcggcg gcgctcaccc tcgagggcgc gggccagctc gggatcgaag
     1021 tcggcgatgg tctcgttgct gcggaacatg cggatgcgcc tcggaggaaa gccaggcgga
     1081 aacgggctgt aaattgtacc ggaaggcccc cgctgccgcc ttgtcgtgaa cgcgcctcct
     1141 gcacagaacc cgcgcgacag cggctcaggt ctacagcagg ttttccagca ccgcgttcca
     1201 ggcctgggcg aggttgccgc ggttgtagcc cccgcccacg agcgcgagca cacggccgtg
     1261 accgaggtta tcggcgagct ccttcagcga gcgggccgcg tgcccgtgcg agcgggccga
     1321 gaggcgcagg tgcgtgatcg gatcgccctc gaggctgtcg gccccacact gcaggatgag
     1381 gaactcgggc tcgaagcgct cgagatgcgc caacacgcgc ggccacacct gctcgaagac
     1441 cgagtcatcg gcccccggcg gtagcggcac gttgagcttc atgcccgcgg cggccccacg
     1501 gccggtctcg ccgctcgctc ccgtgccggg gtagaggtag cgtccgtcct cgtgcaggtc
     1561 ggcgaacacc accgcggcgt cgtcctcgaa ggcgtagaac accccgtcgc cgtggtgcgc
     1621 gtcgatgtcg acgtaagcga cacgcctcaa gccggcgcgc ttcagcagct cgatcgcgac
     1681 gccacagtcg ttgaagacgc aaaagcccgc ggcgcgctcg cgcgccgcgt gatggagccc
     1741 ggcgatcggc acgaaggcgc gccggcagtc gccgcgcatg atcgcctcca tcgcagcgag
     1801 cgtcgcgccg acgacacccg cggccgcctc gtacacgccg cggaacgccg gcgtgtcgcc
     1861 ggcgtcgaga agcccggtgc cgctcacgga gcgctcgcgt acgaactcga ggtacgccgg
     1921 gctgtggaac gcgcgcagct cctcttcggt ggcggtgcgt ggctcgagca cctgcacgcg
     1981 ccggtcgagc ccacgcgcct cgtactcgcg catgaaggcg gcaagccggt caggcccgaa
     2041 ggggtgtcca tccccgaagc tgtagcgggc gagctgctcg ctcaccacca cccccacgcc
     2101 ggcattcatg tgcgcccgca acccccgcgg tcattctccc tggacgcagc ctagcacagc
     2161 gggaccgggg caaggcccgc gggcgcgacc ccgtataatc gcgccctccc ctgactgctc
     2221 ggtcgttgca tggctcagta catctatacg atgaatcgcg tgtcgaaggt ggtcccgcct
     2281 aagcgggtga ttctgcgcga catcagcttg tcgttcttcc ccggcgcaaa aatcggtgtg
     2341 ctgggcctga acggcgccgg caagtcctcg ctcctgcgga tcatggcggg cacggatcag
     2401 gagttcgacg gcgaggcgcg cccgcagagc ggcatccgca tcggcttcct gccgcaggag
     2461 ccgcagctcg acgcctcgcg cgacgtgcgc accacggtca tggaaggcct cggcgagacg
     2521 ttccgccagc tcgaggagtt caatcacatc agcgagcgtt ttgcgcaaga gctgtccgag
     2581 gaggagatgg ccaagctcct cgagcgccag gcgcagctgc aggatgcgat cgacgccgcc
     2641 ggcggctggg agctcgagcg caagctcgag atcgccgccg atgcgctgcg cctgccgccg
     2701 tgggaagcga gcgtcgccac gctctcgggc ggtgagcggc gccgcgtggc gctctgtcgc
     2761 ctgctgctct cggctccgga catgctgctg ctcgatgagc cgaccaacca tctcgacgcc
     2821 gagtcgatcg cctggctcga gcgctatctc gaggaatatc cgagcaccgt gatcgcggtc
     2881 acccacgacc gctacttcct cgacaacgcc gcgcagtgga tcctcgagct cgaccgcggc
     2941 tacggcatcc cctgggaagg aaactactcg tcgtggcggg agca
//
LOCUS       DATLKL010000871         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_67107, whole genome shotgun sequence.
ACCESSION   DATLKL010000871 DATLKL010000000
VERSION     DATLKL010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67107"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..799
                     /locus_tag="VM713_11830"
     CDS             89..799
                     /locus_tag="VM713_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84991.1"
                     /translation="MSSYIDSASRLHHDQDRNLVVTCPHCQTVSHITQSAVPRFEDLQ
                     LYRPKQIGVVYLCDACHAPIFLRFTVRVYGASRIELSPQFTEVERAREKFSFTYIPEE
                     VETLFREALLCYTHDAFNAFASMCRRTAHAIFQDLGEAGKLRLFDELNGLRDLTDIAP
                     DLFTRMRNVLFGAELDARASVPLLDGYQAGVMLEVMKDLLYEAYIRKGRLEQAIMVRH
                     FFLDETGSYKVTPLVEAG"
     gene            complement(796..>2036)
                     /locus_tag="VM713_11835"
     CDS             complement(796..>2036)
                     /locus_tag="VM713_11835"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HVH84992.1"
                     /translation="HDRSDGGLLVTLLEMAFAGHCGLDIALPAARGAAAAQLFSEEPG
                     VVVQILADDEPRFLEILGRHGLAAAALYLGAPTADLRVQVRIGTTVIDEAWADLRRAW
                     SETSWRMRHLRDDPQCADEEYAAQSAAADPGLTVDLKFDPQDDIAAPYVGRGARPKIA
                     ILREQGVNSQTETAAVFAQAGFEPHDVHMTDLFSGARTLAEFKGLVACGGFSYGDVLG
                     AGEGWAKSILFHPAVRAEFERFFARADSFALGICNGCQMFAALKSLIPGTAHWPRFVQ
                     NRSEQYEARFPLVEVLPSPSVLLHGMAGSLLPIAVSHGEGRAEFPSPQAAAACAGSGL
                     VGFRYVQHDRSVAATYPANPSGSPFGIAALSNTDGRVTVTMPHPERSFRSLQNPWRPP
                     DAGSYSGWMRLFRNARRFVG"
BASE COUNT          337 a          707 c          688 g          304 t
ORIGIN      
        1 agcggtggct cgcggcgcga tcggttaaca tggggtcgcg cggcgtgacg gacacgaaaa
       61 caacagtccg cgccgcatga tgaagggtat gtcgtcgtac atcgactcgg cgagccgcct
      121 gcatcacgac caggacagga acctggtggt gacctgcccg cattgccaga cggtctcgca
      181 catcacgcag agcgccgtgc cgcgcttcga ggatctgcag ctttatcggc ccaagcagat
      241 cggcgtcgtg tacctgtgcg acgcctgcca cgcgccgatc ttcctgcgct tcaccgtgcg
      301 cgtgtacggg gcgtcgcgta tcgagctgtc accgcagttc accgaggtgg agcgggcgcg
      361 cgagaaattc agcttcacct acattccgga ggaggtcgag acgctgttcc gcgaggccct
      421 cctctgctac acgcacgacg ccttcaacgc cttcgcctcc atgtgccgcc gtacggcaca
      481 cgcaatcttc caggacctcg gcgaggcggg gaagctgcgc ctcttcgatg agctcaacgg
      541 gctgcgcgat ctcaccgata tcgccccgga tctctttacc cgcatgcgca acgtgctgtt
      601 cggcgccgag ctcgacgcgc gcgccagcgt gccgctgctc gacggctacc aggcgggcgt
      661 catgctcgaa gtgatgaagg acctcctgta cgaggcctac atccgcaagg gccgcctgga
      721 gcaggcgatc atggtgcggc acttcttcct cgacgagacc ggcagctaca aggtcacccc
      781 gctggtcgag gcaggctagc cgacaaaccg ccgcgcgttg cgaaacaggc gcatccagcc
      841 gctgtagctg ccggcgtcgg gcggccgcca gggattctgc agagagcgga acgagcgctc
      901 cgggtgcggc atggtgacgg tgacgcgccc atcggtattg ctgagcgcgg cgatgccgaa
      961 ggggctaccg ctcgggttgg cgggataggt agcggcgacg ctgcgatcgt gctgcacgta
     1021 gcggaaaccg accaggccgc tccccgcgca ggccgcagcc gcctgcggcg agggaaactc
     1081 cgcccgccct tccccgtgcg acaccgcgat cggcaggagc gagccggcca tcccgtgaag
     1141 cagcactgac ggcgagggca gcacctccac cagcgggaaa cgcgcctcgt actgctcgct
     1201 gcgattctgc acgaagcgcg gccagtgggc cgtgccggga atcaagctct tgagcgcagc
     1261 aaacatctgg cagccgttgc agatgccgag cgcaaagctg tcggcgcggg caaagaagcg
     1321 ctcgaactca gcgcgcaccg cggggtgaaa caggatcgac ttggcccacc cctcgccggc
     1381 tccgagcacg tcgccgtagc tgaagccgcc gcaggccacg agtcccttga actcagcgag
     1441 cgtgcgcgcg ccggagaaca ggtccgtcat gtgcacgtcg tgcggctcga agcccgcctg
     1501 agcgaacacc gccgcggtct ccgtctggct gttgacgccc tgctcgcgca ggatcgcgat
     1561 ctttggccgc gcgccgcggc cgacgtaggg cgcggcgatg tcgtcctgcg ggtcgaactt
     1621 gaggtccacc gtgaggcccg ggtcggctgc cgcgctctgc gcggcatatt cctcgtcggc
     1681 gcactgcgga tcatcgcgca agtgccgcat gcgccacgag gtctcggacc aggcgcggcg
     1741 caagtccgcc cacgcctcgt cgatgaccgt cgttccgatc cgcacctgca cgcgcaggtc
     1801 cgccgtcggt gcgccgaggt acagcgccgc cgcggcgagg ccgtgccgcc cgaggatctc
     1861 gaggaagcgc ggctcatcgt ccgcgaggat ctgcacgacc acgccgggct cctcgctgaa
     1921 gagctgcgcc gcagccgctc cgcgcgcggc cggcagcgcg atatcgaggc cgcagtgccc
     1981 cgcgaaggcc atctcgagga gcgtgacgag cagcccgccg tctgagcggt cgtgat
//
LOCUS       DATLKL010000872         1552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_179903, whole genome shotgun sequence.
ACCESSION   DATLKL010000872 DATLKL010000000
VERSION     DATLKL010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179903"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..473)
                     /locus_tag="VM713_11840"
     CDS             complement(9..473)
                     /locus_tag="VM713_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84993.1"
                     /translation="MVLALLAACTPVTTRPDFRPDPRALVVILDARPERVTTELATLV
                     AAESLVVERVNIRDGYLESRWYDPPSHRSYRRDRDIGDLGAAVKVRCWADPYVPGQTR
                     LTIEAVYRPRYDPSRVERDLEVAVPKEHVGYGIAEHLFAKIKQKVGVPKTGS"
     gene            complement(552..1151)
                     /locus_tag="VM713_11845"
     CDS             complement(552..1151)
                     /locus_tag="VM713_11845"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HVH84994.1"
                     /translation="MFGYDWPRFHAAVNDLPAALLFVTVLFDFGAWLTRRESLKAAAL
                     WTLWAGAIGGWIAVIAGLEAEDVIEHGDALHDLMGTHKILAITTMCIFTAALGWKLLR
                     RGRLAPAEELGVRVLSLVGLVGIVWTGMVGGRMVFDHAAGVPSATMQVEIRDRAAEHH
                     HHDAEDEHDTPNAPPRSPAESATATPHSRPAAGAKPPQH"
     gene            complement(1189..>1552)
                     /locus_tag="VM713_11850"
     CDS             complement(1189..>1552)
                     /locus_tag="VM713_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HVH84995.1"
                     /translation="GKQPVTAKDSSGFIVNLLLVPYLLDAIRAVERGVGSVPDIDKAM
                     QLGCGYPMGPLTLLDFVGLDTTYKIAEIMFTEYREQRYAPPPLLKRMVIAGLNGRKSG
                     KGFYDYSAEPPKVVDLGL"
BASE COUNT          257 a          500 c          543 g          252 t
ORIGIN      
        1 agtgggagtc aactgcccgt ctttggcacg ccgacctttt gcttgatctt tgcaaacaga
       61 tgctccgcga tcccgtagcc aacgtgctcc ttcggcacgg cgacctcgag atcccgctca
      121 acccgcgacg ggtcatagcg cgggcggtac accgcttcga tcgtcaggcg ggtctgcccg
      181 ggcacgtagg gatcggccca gcagcggacc ttgaccgcag cgccgagatc cccgatgtcg
      241 cggtcgcgtc ggtaggagcg gtggctgggt gggtcgtacc agcgcgattc gaggtagccg
      301 tcgcggatat tgacccgctc caccacaaga ctctccgctg cgacgagcgt cgcgagttcc
      361 gtagtcaccc gctccgggcg agcgtcgaga atgacgacga gtgcccgagg gtcgggccgg
      421 aagtccggcc gcgtcgtgac gggggtgcaa gcggcgagca gcgcgagcac cacgcgcggg
      481 agcggcggcg agcgccgtga gccgcgcgac ggagccccga caccgggcgt ccgtgggtcg
      541 cccgcgtgca tctagtgctg aggcggcttc gcgccagcag cagggcggct atgcggcgtg
      601 gcggtggcgc tctcggccgg ggagcgcggc ggggcgttcg gagtgtcgtg ctcgtcctcg
      661 gcatcgtgat ggtgatgctc tgccgcccga tcgcggatct ccacttgcat cgtggctgac
      721 ggaacgccgg cggcatggtc gaacaccatc cgtccaccga ccatgccggt ccagacaatc
      781 ccaacgagtc cgaccaggct gagcacccgc acgccgagtt cctcagcggg agcgagccgg
      841 ccccgccgca agagcttcca gccgagagcg gcggtgaaaa tgcacatcgt ggtgattgca
      901 aggatcttgt gtgtccccat caggtcatga agcgcgtcgc cgtgctcgat cacgtcttcg
      961 gcttcgagcc cggcgatgac ggcgatccaa ccgccgatgg cgcccgccca cagcgtccac
     1021 agcgccgccg ccttgagcga ttcgcgccgc gtgagccagg ccccgaaatc gaacaacacc
     1081 gtcacgaaga gcagggccgc ggggaggtcg ttcaccgctg cgtggaagcg gggccagtcg
     1141 tagccaaaca tggggatggg ttccttaggg gtaggggtcg gaacagctct agaggcctaa
     1201 gtcgacgacc ttgggcggtt cggcggagta gtcgtagaag cctttgccgc tcttgcggcc
     1261 gttcaggccg gcgatcacca tgcgcttcag gagcggcgga ggcgcgtagc gttgctcgcg
     1321 gtactcggtg aacatgattt cggcaatctt gtaggtcgtg tcgaggccga cgaagtcgag
     1381 cagggtgagg gggcccatcg ggtagccgca gccgagctgc atcgccttgt cgatgtccgg
     1441 cacgctcccg acccctcgct ccaccgcccg gatcgcgtcg agcagatacg ggacgagcag
     1501 caaattaacg atgaacccgg aactgtcctt cgcggtgacg ggttgcttcc cg
//
LOCUS       DATLKL010000873         1630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_123607, whole genome shotgun sequence.
ACCESSION   DATLKL010000873 DATLKL010000000
VERSION     DATLKL010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123607"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VM713_11855"
     CDS             <1..1312
                     /locus_tag="VM713_11855"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVH84996.1"
                     /translation="CRFGSSPEDPNSPGLTLLRRLQNQPAAPAVIAIAEDGNELTAVR
                     AMQLGALDYLPKKLLSPERLSTAVLVALRRVERRVQKRLASLQHLEHDRTVQAARSAP
                     SATETGSATRPPGWFAEAVAGAVPTPPVLQPAAGKAPAPATEALQPRGIVSLLTAPAG
                     EDFIPGCTLRLKVGESEKAAVYLATSAARGHNVALKVSKTLRDEAAGRQFLEREYTAV
                     QAIKSPLVVEIYDYGIHAGFEYLAMEYLPRGDLKARIARGVSERESLQYLQRIAEALQ
                     VVHQAGLVHRDLKPPNVMLRENEAVALIDFGLARQIDGSDSSVQSGQTGVLRGSPYYM
                     SPEQALGDQLDGRSDFYSLGIILCEMLTGQKPYTGGAALDVLQQHVSAPPPKLPDALA
                     RYQPLLTLLVAKSRSERFATAAEIINAAGALLASTVAEPQSSAA"
     gene            complement(1334..>1630)
                     /locus_tag="VM713_11860"
     CDS             complement(1334..>1630)
                     /locus_tag="VM713_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH84997.1"
                     /translation="VVVYAVRRVADYALTRPCRDALYTVVSREEKYKAKSLIDTFVYR
                     GGDALSGSLYKGLTGSFGAGPAAIGWLGAAVSAVWTVLALALGRAQERAKAGSL"
BASE COUNT          255 a          609 c          532 g          234 t
ORIGIN      
        1 ctgccgcttc ggctcgagcc ctgaggatcc caattccccc gggctgacgt tgctgcgccg
       61 tctgcagaat cagcccgccg cccccgcggt catcgcgatc gccgaggacg gcaacgagct
      121 caccgccgtg cgcgccatgc agctcggcgc gctcgattac ctgccgaaga agctcctcag
      181 cccggagcgc ctcagcactg ccgtgctggt ggcgctgcgc cgcgtagagc ggcgcgtgca
      241 gaaacgcctg gcgagcctcc agcacctcga gcacgaccgg acggtgcagg ccgccaggag
      301 cgctccttcc gcgaccgaga ccggcagcgc aacccgccct ccgggctggt tcgccgaagc
      361 cgtcgcgggc gcggtaccga cgccccccgt cctgcagcct gctgccggca aagcgccggc
      421 cccggcgacc gaggcgctgc agccgcgagg catcgtttcc ctgctgacgg cgcctgcggg
      481 cgaggatttc attcccggct gcacgctgcg gttgaaagtc ggcgagtcgg agaaggccgc
      541 cgtctatctc gccaccagcg ccgcgcgcgg acacaacgtc gcgctgaagg tatccaagac
      601 gctgcgcgac gaggccgccg gccggcagtt cctcgagcgc gagtacaccg ccgtgcaggc
      661 catcaagagc ccgctggtgg tggagatcta cgactacgga atacacgccg gcttcgagta
      721 cctcgcgatg gagtatctgc cgcgcgggga tctgaaggcg cgtattgccc gcggtgtcag
      781 cgagcgcgag tcgttgcagt atctgcagcg catcgccgag gctctgcagg tcgtgcacca
      841 ggcgggcctc gtgcaccgcg acctgaagcc gcccaatgtc atgctgcgcg agaacgaggc
      901 ggtggcgctc atcgacttcg gcctggcgcg ccagatcgac ggcagcgact cgagcgtcca
      961 gagcggccag accggtgtgc tgcgcggctc tccctattac atgagtccgg agcaggcgct
     1021 cggcgatcag ctggacggcc gctccgactt ctatagtctc ggcatcatcc tctgcgagat
     1081 gctcaccgga cagaaaccct acaccggcgg cgccgcactc gatgtgctcc agcagcacgt
     1141 cagcgcgccg ccgcccaagc tgccggacgc cctcgcccgt tatcagccgc tgctcacgct
     1201 cctcgtggcg aaatcgcgct ccgagcgctt cgcgaccgcc gcggagatca tcaacgccgc
     1261 cggcgcgctc ctggcctcga cggtggccga gccgcagtcg agcgcggcgt aacgcggctc
     1321 gcgacgcgct cgttcacaga ctacccgcct tggcgcgctc ctgcgcgcgg ccgagcgcga
     1381 gcgccagcac cgtccacacc gccgagaccg cggctcccag ccagccgatc gccgccggcc
     1441 ctgcgccgaa gctgccggtc agtcccttgt agagcgagcc cgagagcgca tcgcccccgc
     1501 ggtacacgaa ggtgtcgatc aggctcttgg ccttgtactt ctcctcgcgg ctcaccacgg
     1561 tgtacagagc gtcgcggcac gggcgcgtga gggcgtagtc cgcaacgcgg cgtactgcat
     1621 aaaccaccac
//
LOCUS       DATLKL010000874         1501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_224590, whole genome shotgun sequence.
ACCESSION   DATLKL010000874 DATLKL010000000
VERSION     DATLKL010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224590"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1060
                     /locus_tag="VM713_11865"
     CDS             137..1060
                     /locus_tag="VM713_11865"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HVH84998.1"
                     /translation="MIEPRGFGYNPDTARTNTFQRAEDERGGPADPAAAQAQALAEFR
                     KLRQALESEGVEVCAIEDTASPVKPDAVFPNNWLSFHEDGTLVLYPLESATRRPERRQ
                     DVIEAVTRRLHFKVAHLLDLTHFEGEGMFLEGTGSLVLDHAGRVAYACASPRTHPALA
                     AEWARELHYEPVIFQAADRAGVPLYHTNVLMCIGERFAVVGTEAIAAADRQRVLERLR
                     AGGRETIEIGQAEIAQFAGNMLELGTWDEALGDSRVLVMSEAARRSLSPETYARLSGC
                     TDLVLAVPVPTIERLGGGSVRCMLAEVFGHT"
     gene            1057..>1501
                     /locus_tag="VM713_11870"
     CDS             1057..>1501
                     /locus_tag="VM713_11870"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HVH84999.1"
                     /translation="MIELIVKALLAYVLGSCVGSLLIGKLRGVDIRTMGSGNAGATNM
                     LRTQGKVAALAVLLIDLGKGWVATGIIAPWVSPGLPPAEPGIRAWLQAGCGLAVIVGH
                     VYPLWYGFRGGKGVASFLGAVLGINPWLVPVMALTWLAVVVLSGFV"
BASE COUNT          222 a          522 c          507 g          250 t
ORIGIN      
        1 tgctcgatgt catggaatcc accggcacgc aggatgcccg cccgctgcgc ctcatcaact
       61 agccgacgtg cgagaatggg ccatggagcc cagcgcccgt cgttcctccg gccaacagtg
      121 cgccgacgcc gtgctcatga tcgagcctcg cggcttcggc tataacccgg acaccgcccg
      181 taccaacacc tttcagcgtg cggaggatga gcgtggcggg ccggccgatc ctgccgcagc
      241 ccaggcgcag gcgctcgcag agttcaggaa gctccgccag gcgctcgaga gcgagggggt
      301 cgaggtctgc gcgatcgagg acaccgcgag cccggtcaag ccggacgccg tgttccccaa
      361 caactggctc tcctttcacg aggacggcac gcttgtcctc tatcccctgg agagcgccac
      421 gcgccgcccc gagcggcgtc aggacgtgat cgaggcggtc acccggcgac tgcacttcaa
      481 ggtggcgcat ctccttgacc tcacgcactt cgagggcgag gggatgttcc tcgaaggcac
      541 cggcagcctg gtgctcgacc acgccggacg cgtggcgtat gcctgcgctt ccccgcgcac
      601 gcatccggca ctcgccgccg agtgggcccg ggagctgcat tacgagccgg tgatctttca
      661 ggccgcggac cgcgccggcg tgccgctcta tcacacgaat gtcctcatgt gcatcggcga
      721 gcgctttgca gtcgtcggaa ccgaggcgat tgccgccgcc gatcggcagc gggtgctcga
      781 acggctgcgc gccggcgggc gcgagaccat cgagatcggc caggcggaga tcgcgcagtt
      841 cgccggcaac atgctcgagc tcggcacctg ggacgaggcg ctcggggact cgcgggtgct
      901 cgtcatgtca gaggccgccc gccgctcgct gagccctgaa acctacgcgc ggctctcggg
      961 ctgcacggac ctggtgctcg cggtgccggt tcccacgatc gagaggctag gcggcgggag
     1021 cgtgcgctgc atgctcgccg aggtgttcgg acacacgtga tcgagctcat cgtcaaagcg
     1081 ctcctcgcct atgtcctcgg gtcctgcgtc gggagtctcc tcatcggcaa gctccgcggc
     1141 gtcgacattc gcaccatggg aagcgggaat gcgggcgcca ccaatatgct ccgcacccag
     1201 ggcaaggtcg cagcgctggc ggttctgctc atcgatctcg gcaagggctg ggtcgcaacc
     1261 ggaatcatcg ctccctgggt gagcccggga ctccccccgg cggagcccgg gatacgcgcc
     1321 tggctccagg cgggatgcgg gctcgccgtg atcgtcggac acgtgtaccc gctctggtac
     1381 ggtttccggg gcggcaaggg cgtcgcctcg ttcctcggtg ccgtgctcgg catcaacccg
     1441 tggctcgtgc ctgttatggc gctcacctgg ctcgcagtcg tcgttctttc cggcttcgta
     1501 g
//
LOCUS       DATLKL010000875         1894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_33365, whole genome shotgun sequence.
ACCESSION   DATLKL010000875 DATLKL010000000
VERSION     DATLKL010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33365"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VM713_11875"
     CDS             complement(<1..866)
                     /locus_tag="VM713_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85000.1"
                     /translation="MRQLGFLKLNLRPDGGAPDPEHRVIELFRNRAELKKAYGGLQEE
                     SYRLKDLLKQQEAATQRVQDMLNELEERLSGNETAYSTLAFYQLRRMWQNGRELIQQF
                     VADLARQQDERERKLHLAHHNRQQFARRQIAEGAAAAARSLSDEATALVAQLESEHRK
                     LTRFWHYFKRKALERRLHGAQAAATAASGSLAQAQSELEAVNQETLPEFPGLSLDARR
                     AINLAAIAYAWVLCQRLNVIEKPLLQMAREATGRRTAADVYGSPKECVLLMAQISRAQ
                     RLLPARDALAEE"
     gene            complement(904..1203)
                     /locus_tag="VM713_11880"
     CDS             complement(904..1203)
                     /locus_tag="VM713_11880"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HVH85001.1"
                     /translation="MTERSPYTSTLALSYFLPEPAGAGRYPDMRLRIYHDAHLVEAQQ
                     WATVHTQPLLKALRTRAERELDQRWARNVMLNKWLEYCVERGHRFSSRTRLTAGA"
     gene            complement(1200..1382)
                     /locus_tag="VM713_11885"
     CDS             complement(1200..1382)
                     /locus_tag="VM713_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85002.1"
                     /translation="MLANDTLTRVSWRARPRSFVALMGLYESNFIRLGWLAGSLGALT
                     GCTAPRSPETVISFSP"
     gene            complement(1496..>1894)
                     /locus_tag="VM713_11890"
     CDS             complement(1496..>1894)
                     /locus_tag="VM713_11890"
                     /inference="COORDINATES: protein motif:HMM:NF015575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HVH85003.1"
                     /translation="DYAHADVVLIGVSRVGKTPTCVYMALQYGVFAANYPLTEDDLES
                     GKLPARLEPYRAKLFALTIKPERLQQIRAERRPESRYASRVQVQYELRAAEALFARYS
                     IPSLETTERSIEEIASRILNTTGIERRLRP"
BASE COUNT          288 a          623 c          646 g          337 t
ORIGIN      
        1 atctcctcgg ccagcgcgtc acgcgccggg agcagccgct gtgcgcggct gatctgcgcc
       61 atcagcagga cgcattcttt gggggagccg tagacatcgg ccgcggtgcg ccgcccggtc
      121 gcctcgcgcg ccatctgcag cagcggcttt tcgatcacgt tgaggcgctg gcacagaacc
      181 caggcataag caatcgccgc gaggttaatc gcacggcgcg catcgagcga caaccccgga
      241 aactccggga gcgtctcctg attgaccgct tcgagctccg actgcgcctg ggcgagtgag
      301 ccactcgcgg ccgtcgcggc cgcctgcgcg ccgtgcaggc ggcgctcgag agccttgcgc
      361 ttgaagtagt gccagaagcg cgtgagctta cggtgctcgc tctcgagctg cgcgaccagg
      421 gcggtggctt cgtcgctcag gctgcgtgcc gcggccgccg cgccctcggc tatctgccgg
      481 cgcgcgaact gctggcggtt gtggtgcgcg agatggagct tgcgctcgcg ctcatcctgc
      541 tggcgcgcca ggtccgcgac gaactgctgg atgagctcgc gcccgttctg ccacatgcga
      601 cgcagctgat agaacgcgag tgtcgagtag gcggtctcgt tgccgctgag gcgttcctcg
      661 agctcgttca gcatgtcctg gacacgctgg gtggccgctt cctgctgctt gagcaggtcc
      721 ttgaggcggt agctctcctc ctgcagcccg ccataggcct tcttgagctc cgcgcggttg
      781 cggaacagct cgatgacccg atgctcgggg tcgggggcac cgccgtccgg gcgcaggttg
      841 agcttgagga acccgagttg ccgcaaagct ttcatgagaa attgggcggg tgaccgcccc
      901 ttctcatgcg ccggcggtga gcctcgtgcg ggatgagaag cgatgacctc gctccacgca
      961 gtattccagc cacttgttta acatcacgtt gcgggcccag cgctgatcca gttcacgctc
     1021 cgcgcgggtg cgcagcgctt tgagcagcgg ctgggtgtga accgtggccc attgctgcgc
     1081 ctcgaccaga tgcgcgtcgt ggtagatgcg caggcgcatg tccggatagc ggccggcacc
     1141 cgcgggctcg ggcaggaaat agctcagcgc gagggtgctc gtgtagggcg agcgctcggt
     1201 cacggtgaga aggagatcac agtctccggc gaccgtggag cggtgcagcc cgtgagcgct
     1261 cccaggctcc cggccagcca gcccaggcgg atgaagttgc tctcgtagag ccccatcagc
     1321 gcaacgaagc tgcgcggccg ggcacgccag gaaacgcgag tcagtgtgtc gttcgcgagc
     1381 acgcaccgac tatagcgctt aaggcccgct gcgcaagccg atgagtgcgc gttttgtcgc
     1441 cgctgcgcta cctgagtcgc ttgcaaattt cgtgcgacct gcgcgccgcg cgcacttaag
     1501 gacgcaatcg ccgctcgatt ccggtagtgt tgagaatccg gctggcgatt tcctcgatcg
     1561 agcgctcagt ggtctcgagc gacgggatcg agtaacgcgc gaacagcgct tcggccgcgc
     1621 gcagctcgta ctgcacctgc acccgcgagg cgtagcggct ctccgggcgt cgctccgcgc
     1681 ggatctgctg cagccgctcc ggcttgatgg tgagcgcgaa cagcttcgca cggtatggct
     1741 cgagccgcgc gggcagcttg ccgctctcga gatcgtcctc cgtgagcgga tagttcgccg
     1801 cgaacacgcc gtactgcagc gccatgtaca cgcaggtcgg cgtcttgccg acgcgcgaca
     1861 ctccgatcag caccacgtcc gcgtgagcgt agtc
//
LOCUS       DATLKL010000876         2292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_202622, whole genome shotgun sequence.
ACCESSION   DATLKL010000876 DATLKL010000000
VERSION     DATLKL010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202622"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VM713_11895"
     CDS             <1..794
                     /locus_tag="VM713_11895"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVH85004.1"
                     /translation="ARRGVARAERIKRAAAMLERVGLGGAAGKRTEELSGGQRQRVAI
                     ARSMVLEPTLLLLDEPLGALDLKLREHMKVELKQLQSAFGTTFVYITHDQSEALVLSD
                     RVGVMNHGRFEQLGSPQELYYSPATSFVAGFVGASNRLSGTVLRHDGALVELDAGGGL
                     KLRVRASAALASGATADAFIRPEAMTLARTAAELPPNQSALTGEVTDMLFDGANSVVL
                     LRESQSRRELRIALPQTGAFADLRPGARICFGFDAERAICFPGQP"
     gene            791..1645
                     /locus_tag="VM713_11900"
     CDS             791..1645
                     /locus_tag="VM713_11900"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVH85005.1"
                     /translation="MRRLTLALLLAPALLWLLALIILPHLDLAMLSFRERVAPREYVP
                     SLAQYRTFFGEPLYWHVFVRTALLSALATVLTLLVAFPIAWIIAKLARGRMSALLFVV
                     CLIPFWVSETVRTLGWMILLRESGVLPGLLVHLGITAQPVELLYHDATILVGLVYTSL
                     LFMVVPLVGSLESLDNSLIEAAYDLGAKGWSIVRTIVIPHAAPGITAGCIVVFMLTLG
                     NYLTPTLLGGKNSLWFTEQIYTQFITRFNWEQGAAFGFLLLALSTAMVWLGLKLSGQR
                     FSDVMRRV"
     gene            1642..>2292
                     /locus_tag="VM713_11905"
     CDS             1642..>2292
                     /locus_tag="VM713_11905"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVH85006.1"
                     /translation="MIPSLPRPAWLRAATALYVGAFLLFLFLPLTVVAVFAFNDAPYP
                     APPWHGFTLDWFLGNEAQGRVGLFRDSELLGSIWTSGIVALWVTGLSVAVGTANAFLM
                     EREQFPGKGILSLLMLTPLVIPGVILGISILAFASRLADLCTDLFGVEADFLRPGLPL
                     VVAGQFSYIVAIATLTISARLKRFDRSLEDAALNLGASQTAVLWHITLPYLRPALIG"
BASE COUNT          294 a          819 c          741 g          438 t
ORIGIN      
        1 tggcgcggcg cggcgtcgcg cgcgccgagc ggatcaagcg cgccgcggcg atgctcgagc
       61 gggtgggact cggcggcgcc gccggcaagc gcaccgagga gctctccggc ggtcagcggc
      121 agcgcgtcgc gatcgcgcgc agtatggtgc tcgagccgac gctactgctc ctcgatgagc
      181 cactcggtgc gctcgatctg aagctccgcg agcacatgaa ggtcgagctc aagcagctgc
      241 agagcgcctt cggcaccacc ttcgtctaca tcacccacga tcaatccgag gcgctggtgc
      301 tgtcggaccg cgtgggcgtc atgaaccacg gccgcttcga gcagctcggc agcccgcagg
      361 agctctacta ctcccccgca acttccttcg tcgccggctt cgtcggggcc agcaaccgcc
      421 tgagcggcac ggtgctcagg cacgacggcg cgctggtcga gctggacgcc ggcgggggtc
      481 tcaagctccg cgtgcgtgcc agtgccgcgc tcgcaagcgg cgcgactgct gatgcgttca
      541 tccgtcccga ggccatgacg ctggcccgca cggcggccga gctgccgccg aaccagagcg
      601 cgctgaccgg cgaggtcacg gacatgctgt tcgacggggc gaactcggtg gtgctgctgc
      661 gtgagtcgca gagccgccgc gagctgcgca tcgcgctccc gcagaccgga gcgttcgcgg
      721 acctccggcc tggtgcgcgc atctgcttcg gcttcgatgc cgagcgggca atctgctttc
      781 caggtcagcc atgagaaggc tcactctcgc gctgttgctc gcgccggcgc tcctgtggct
      841 gctcgcgctc atcatcctgc cgcacctcga cctggcgatg ctgtcgtttc gcgagcgcgt
      901 cgcgccgcgc gagtacgtgc cgagcctggc ccagtaccgc acctttttcg gcgagccgct
      961 ctactggcat gtgttcgtgc gcacggcgct gctctcggcg ctcgcgacgg tgctgacgct
     1021 gctggtcgcc tttccgatcg cctggatcat cgccaagctg gcgcgcgggc gcatgtcggc
     1081 gctgctgttc gtcgtctgcc tcatcccttt ctgggtgagc gagacggtgc ggacgctcgg
     1141 gtggatgatc ctgttgcgtg aatcgggcgt gctgccgggg ctgctcgtgc atctcggcat
     1201 caccgcgcag cccgtcgagc tcctctacca cgacgcgacg atcctcgtcg gcctcgtcta
     1261 tacgtcgctc ctcttcatgg tggtgccgct ggtcggcagc ctcgagagtc tcgataactc
     1321 cctcatcgag gccgcctatg acctgggcgc caagggctgg tcgatcgtgc gcacgatcgt
     1381 catcccgcac gccgcgcccg gcatcaccgc cggctgcatc gtggtgttca tgctgaccct
     1441 cggcaactac ctgacgccga ccctgctcgg cggcaagaac tcgctgtggt tcaccgagca
     1501 gatctacacg cagttcatta cccgcttcaa ttgggagcag ggcgcggcct tcggcttcct
     1561 gctgctcgcc ctctcgactg cgatggtgtg gctgggactg aagctctcgg gacagcgctt
     1621 cagcgacgtc atgaggcgcg tgtgataccg agcctccccc gtcccgcctg gctgcgtgcc
     1681 gcgactgcgc tctacgtggg cgcttttctc ttattcctct tcctgccgct caccgtggtg
     1741 gcggtgtttg ccttcaacga cgcgccctac cccgctcccc cctggcatgg cttcacgctc
     1801 gactggttcc tcggcaacga agcgcaggga cgcgtcgggc tgtttcgcga ctccgagctc
     1861 ctcggcagca tctggacgag cggcatcgtc gcgctatggg tcacgggact ctcggtggcc
     1921 gtcggtaccg ccaacgcgtt cctgatggag cgcgagcagt ttcccggcaa gggcatcctg
     1981 tcgctgctga tgctgacgcc gctggtcatc ccgggcgtca tcctcggcat ctcgatcctc
     2041 gcgttcgcga gccgcctcgc ggacctgtgc accgatctct tcggggtgga ggcggatttt
     2101 ctccgcccgg ggttaccgct cgtcgtggcg ggccagttct cctacatcgt cgcgatcgcc
     2161 acgctcacca tcagcgcgcg tctcaagcgc ttcgaccgca gcctcgagga cgccgccctg
     2221 aacctcggcg cctcgcagac tgcggtgctg tggcacatca cgctgccgta cctgcgtccc
     2281 gcgctcatcg gc
//
LOCUS       DATLKL010000877         2427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_10613, whole genome shotgun sequence.
ACCESSION   DATLKL010000877 DATLKL010000000
VERSION     DATLKL010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10613"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..430)
                     /locus_tag="VM713_11910"
     CDS             complement(23..430)
                     /locus_tag="VM713_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85007.1"
                     /translation="MKALTKASMVAVAALWAGAVLAESPGATAQASHMEKLAVLLDLT
                     DAQKPQVATILQGEHAQMKALMEQTKAAGAKPDFEALRAAHTQIQQDTLTKLSSVLSP
                     TQVKKFQTLQQMHHGFGHRGPPGGAPAAVPQSN"
     gene            complement(556..1923)
                     /locus_tag="VM713_11915"
     CDS             complement(556..1923)
                     /locus_tag="VM713_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH85008.1"
                     /translation="MLLAQAVGLVLIAEERETFVLQSSVREWTRRITETVQLLEPLSS
                     AERAAAVAQLADPRGMPPSLPPPPADDPPRAARNLLDRGRTFVRLPVLSDFERTLREQ
                     VQAALGARFEVTVGRTLDASRAAIALPVPFYEAREIAAHQNTAQRYDVMVHFPDGATE
                     LFRVTRMPGGAPLPRNLRVNLTLLVLILVIALYVTARSITRPLSALARAADRVGREAL
                     PEMLPEAGARELRDAARAFNTMQDRLRRYLDSRSRVLAAMSHDLKTPLTRLRLQVETL
                     EDLSLQTRMVKELTEMELMVREALALFRGLDDGEPAGPVDVNELLAGVQQAFSEMGAA
                     VTVRGRALAPFTGKAQALKRCLTNLISNAVNFGTRATVLLEDDGELVIRVRDEGPGIP
                     AAELERVFEPFHRLEHSRNRDSGGTGLGLTIARDIAQAHGGTLLLTNLPPGGLEAALR
                     LPRRR"
     gene            complement(2000..>2427)
                     /locus_tag="VM713_11920"
     CDS             complement(2000..>2427)
                     /locus_tag="VM713_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVH85009.1"
                     /translation="DDYVAKPFYPRELVGRIKAVLRRSAHAPRDPSPESVRCFSFGGW
                     RLDTITRTLTDAEGKEVALSGAEYRLLAVLLAAGNRVLTRAQLTELLRGRDADPFDRS
                     IDVRISRLRQILGDDARAPQIIKTVYGEGYVVGVSVESQ"
BASE COUNT          339 a          822 c          890 g          376 t
ORIGIN      
        1 gcgctgccgc gctgataccc tctcagttgc tctgcggaac ggcggcgggc gcaccgcccg
       61 gcgggccgcg atggccgaag ccgtggtgca tctgctgtag cgtctggaac ttcttcactt
      121 gggtgggcga gagcacgctc gagagcttcg tgagcgtgtc ctgctggatc tgcgtgtgcg
      181 ccgcgcgcag cgcctcgaag tcgggcttgg cgccggccgc cttcgtctgc tccatcagcg
      241 ccttcatctg tgcatgctcg ccctggagga tcgtcgcgac ctgcggcttc tgcgcatccg
      301 tcagatcgag caggacggcg agcttttcca tgtggctcgc ttgcgctgtg gccccagggc
      361 tctcggccaa cacggcgccc gcccacaatg cggcgactgc caccatcgaa gccttcgtca
      421 acgctttcat gaacgcttct cctcgtgcgg gtgcgaaaac tcacgggttg caccgcagcg
      481 ttagcctagc gggggcgtgc agacgggcgc cgtgagcaac tgtgagggag tgtcaacccg
      541 gcgctgcggg cccgttcagc gtcgccgcgg cagccgcagt gccgcctcga ggccgcccgg
      601 cgggaggttc gtgaggagga gcgtgccgcc gtgcgcctgg gcgatgtcgc gggcgatggt
      661 gagacctaac cccgtgccgc cgctgtcgcg gttgcgcgag tgctcgagcc ggtggaaggg
      721 ctcaaatacc cgctcgagct ctgccgcggg gatccccggc ccctcatcgc gcacccggat
      781 gacgagctca ccgtcgtcct cgagcagtac ggtggcgcgc gtgccgaagt tgacggcgtt
      841 ggagatgaga ttggtgaggc agcgcttgag ggcctgcgcc ttgccggtaa agggcgccag
      901 cgcccggccc ctcaccgtca ccgcggcacc catctcggag aaagcctgtt gcacgcccgc
      961 gagcagctcg ttcacatcga cgggcccggc gggctcgccg tcgtcgagtc cgcggaagag
     1021 cgcgagtgcc tcgcgcacca tgagctccat ctcggtgagc tccttcacca tgcgtgtctg
     1081 gagcgagagg tcctcgagcg tctccacctg cagccgcagc cgcgtgagcg gcgtcttcag
     1141 gtcgtgcgac atcgccgcga gcacgcggct gcggctgtcg agatagcggc gcaggcgatc
     1201 ctgcatggtg ttgaaggcgc gtgccgcatc gcgcagctcg cgcgcgccgg cctcggggag
     1261 catctccggc agggcctcgc gccccacccg gtcggcggcg cgggccagcg ccgagagcgg
     1321 gcgggtgatg ctgcgtgcgg tgacgtagag cgcgatcacg aggatcagca cgagcagcgt
     1381 cagattgacg cgcaggttgc gcggcagcgg cgctccgccc ggcatgcggg tgacgcgaaa
     1441 gagctccgta gcgccgtccg ggaaatggac catcacgtcg taacgctgcg ccgtgttctg
     1501 atgcgcggcg atctcgcgcg cttcgtagaa gggcaccggc agcgcgatcg ctgcgcggct
     1561 cgcatcgagc gtgcgcccaa cggttacctc gaatcgcgcg ccgagcgccg cctgcacctg
     1621 ctcccgcagc gtccgctcga aatcgctgag cactggcagt cgcacgaagg tgcggccccg
     1681 gtcgagcagg ttgcgcgccg cgcgcggcgg gtcgtccgcc ggtggcggtg ggagcgaggg
     1741 gggcatcccg cgcgggtccg cgagctgtgc gaccgccgcg gcgcgctcgg cggagctcaa
     1801 cggctcgagg agctgcacgg tctcggtgat gcggcgcgtc cactcgcgca cgctgctctg
     1861 caggacgaag gtctcgcgct cctcggcgat gagcaccagg cccacggcct gggcgagcag
     1921 caccgcgagc accgtggccg cgatcagccg gccgaggagt gactgcggcc agagtgtcag
     1981 gggcgccgac tttaagggct cactggcttt cgacgctgac gccgacgaca tacccctcgc
     2041 catagacggt cttgatgatc tgcggtgcgc gggcgtcgtc gccgagaatc tgccgcagcc
     2101 ggctgatgcg cacgtcgatg ctgcggtcga aggggtcggc atcgcgcccg cgcaggagct
     2161 cggtgagctg ggcgcgcgtc aggacgcggt tgccggccgc gagcagcacc gcgagcagcc
     2221 ggtactcggc accgctcagg gccacttcct tgccctcggc gtcggtcagg gtgcgggtga
     2281 tcgtgtcgag ccgccagccc ccgaagctga agcagcgcac cgattcgggc gagggatcgc
     2341 gcggggcgtg tgccgagcgg cgcagcaccg ccttgatgcg cccgacaagc tcgcgcgggt
     2401 agaagggctt ggcgacgtaa tcgtcgg
//
LOCUS       DATLKL010000878         1916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_101265, whole genome shotgun sequence.
ACCESSION   DATLKL010000878 DATLKL010000000
VERSION     DATLKL010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101265"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VM713_11925"
     CDS             <1..268
                     /locus_tag="VM713_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85010.1"
                     /translation="FVREVFDLRNHEELERFLGFEYRACCWRLRLGARHYVSSFNGTR
                     DTGIWLQLELAGLAGVGSASDTSLSEEIRGYTPADTSAQKIKAR"
     gene            302..>1916
                     /locus_tag="VM713_11930"
     CDS             302..>1916
                     /locus_tag="VM713_11930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVH85011.1"
                     /translation="MRRIMTAALTLVAVSAAALGVCGRATAQTRELDTRGVMLDRIAA
                     IVNDGVVLNSDLELQIGAVSERLRAQKLELPAQNVLRQQVLERLVVQEIQLQRAQHDG
                     VKVPDETLNSALQDVARRNNLTLSQLPQALAEQGVEYAAYREDLRKEITLSMLRQRDV
                     LERIVVTPKEIDQYLDKEAKKPSGNHEYNVSHILIAVPQEATQTQVDEAARRAGEVYQ
                     KASSGEDFARLAVAYSNSQTALEGGSLGWRKETELPTFLTDVVAKLKPGEVSQPLRTP
                     TGFHLVKLNEVRGADAKSIENQVHLRHILMKTTNLADDATIKLKLNALCERILRGEDF
                     AGLAQTNSEDPGSAAQGGDLGWTGPGSFVTEFEEHTANLKDRRSASRSRPSTAGTSCR
                     CSAIASSTRPTRSSGARPWRRSARASRTRRPRSGCDACGTRRSWNTSRSRRVSGGMLP
                     RIAVTSGEPAGIGPELCLALAAGARDCELVCLADPGLLAERARSLGATVELQPYAAHA
                     QRARQRQTPGTLLVESCALGTASSPGRLDK"
BASE COUNT          347 a          646 c          664 g          259 t
ORIGIN      
        1 gttcgtgcgc gaggtcttcg acctgcgcaa ccacgaagaa ctggagcgat tccttggctt
       61 cgagtaccgt gcgtgctgct ggcggctgcg cctgggcgcg cggcactatg tcagcagctt
      121 caacggcacc cgggataccg ggatctggtt gcagctggaa ctcgcggggc tggccggtgt
      181 cggatcggcg tcggacacct cgctcagcga ggagatccgc ggctacactc ccgcggacac
      241 gagtgcccag aaaatcaagg cccgctgagg cctcttcctg aactcacgag gaactgaagc
      301 gatgcggcgg atcatgacgg cggccctgac cctggtagcc gtgagcgcgg cggcgctcgg
      361 cgtgtgcggg cgcgcaacgg cgcagacccg cgagctcgac acccggggcg tgatgctcga
      421 ccgcatcgcc gccatcgtca acgacggcgt ggtgctcaac agcgacctgg agctgcagat
      481 cggggcggtg agcgagcggc tgcgggcgca gaagctcgag ctgccggcgc agaacgtgct
      541 gcgccagcag gtgctcgagc ggctggtggt gcaggagatc cagctgcagc gcgcccagca
      601 cgacggcgtc aaagtccccg atgagacgct gaactccgcg ctccaggacg tggcgcgtcg
      661 caataacctc accctgtcgc agctgcctca ggccctcgcc gagcaggggg tcgagtacgc
      721 cgcgtaccgc gaggatctgc gcaaggagat cacgctgtcg atgctgcgcc agcgcgacgt
      781 cctcgagcgc atcgtggtca caccgaagga gatcgaccag tacctcgaca aggaggcgaa
      841 gaagccgtcg ggcaaccacg agtacaacgt ctcccacatc ctgatcgcgg tgccacagga
      901 agcgacccag acacaggtcg acgaggccgc gcggcgtgcc ggcgaggtct accagaaggc
      961 ctcgagcggc gaggacttcg cccgcctcgc ggtggcctac tcgaacagcc agaccgccct
     1021 cgagggcggc tcgctcggct ggcgcaagga gaccgagctg ccgaccttcc tcaccgacgt
     1081 ggtcgcgaag ctcaagcccg gcgaggtgag ccagccgttg cgcacgccca ccggtttcca
     1141 cctcgtgaag ctgaatgagg tgcgcggcgc cgacgccaag tccatcgaga accaggtcca
     1201 cctgcgccac atcctcatga agaccaccaa tctcgccgac gatgccacca tcaagctgaa
     1261 gctcaacgcg ctgtgcgagc ggatcctgag aggcgaggac ttcgccggcc tcgcccagac
     1321 caactccgag gatccgggct cggccgccca gggcggggat ctcggctgga cgggacccgg
     1381 tagcttcgtg accgagttcg aggagcacac cgcgaatctc aaggacagga gatcagcgag
     1441 ccgttcaaga cccagtacgg ctggcacatc gtgcagatgc tcggccatcg ccagttcgac
     1501 gcgaccgacg aggtcaagcg gcgcaaggcc atggaggcga tccgcgcgag caagtcggac
     1561 gaggagaccg agatctggct gcgacgcctg cgggacgagg cgttcgtgga atacaagtcg
     1621 tagccggcgg gtcagcggcg gcatgcttcc ccggatcgcc gtcacttcag gagagccggc
     1681 ggggatcggc ccggagctgt gcctggcact cgccgcgggc gcgcgcgact gcgagctcgt
     1741 gtgcctcgcc gaccccggcc tgctcgccga gcgcgcccgc tcgctgggtg ctacggtcga
     1801 gctgcagccc tatgcggcgc acgcgcagcg cgcgcggcaa cgacagacgc cggggacgct
     1861 gctcgtcgaa tcctgcgcgc tcgggactgc gagcagcccc gggcggctcg ataagc
//
LOCUS       DATLKL010000879         1742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_112353, whole genome shotgun sequence.
ACCESSION   DATLKL010000879 DATLKL010000000
VERSION     DATLKL010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112353"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VM713_11935"
     CDS             complement(<1..201)
                     /locus_tag="VM713_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85012.1"
                     /translation="MNPLNNHRQSLVLGIVIAVVLALVITGLNVTFYPLGLARWFHIV
                     AGVFWIGLLYYFNVAQTPALADA"
     gene            305..832
                     /gene="grxD"
                     /locus_tag="VM713_11940"
     CDS             305..832
                     /gene="grxD"
                     /locus_tag="VM713_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HVH85013.1"
                     /translation="MTEAPVRATPPERPPSPCINVCSLDSRGYCIGCLRTGAEIGRWL
                     AMSSEEQWRLIAELEKRRRQLEVVTMDVVERIKSELAAGPVVLFMKGTPDFPQCGFSA
                     QTVAALRAVGANFRYVNIFEEPEIREALKRYSNWPTYPQLYVKGELIGGCDIALEMLK
                     SGELKKLMSEAGAAQ"
     gene            complement(804..1565)
                     /gene="rnt"
                     /locus_tag="VM713_11945"
     CDS             complement(804..1565)
                     /gene="rnt"
                     /locus_tag="VM713_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HVH85014.1"
                     /translation="MSEAGTSPPPEPRTGAELMAGRFRGYLPVVIDVESGGFHAATDA
                     LLEIAAVLIDIDADGTLRRGATHHYHVQPFEGARLDPTSLSVTGIDPHHPLRPAIPER
                     DALQRIFREVRHAVRASGCRRAILVGHNAAFDLAFLNAAVARADVKRNPFHPFSCFDT
                     ATLAGAALGQTVLAKALAVAGLEWDAGSAHNARYDAERCAEVFCLVCNRLRESFASAR
                     ERAAALGWLDAPAGDAAGEDAPLEEAITAPPPPRS"
     gene            complement(1562..>1742)
                     /locus_tag="VM713_11950"
     CDS             complement(1562..>1742)
                     /locus_tag="VM713_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85015.1"
                     /translation="YGADFYRLPRNAATITLVREPWEVPQHYPFGADELVPLRAGARI
                     PWRLAAAAAATRPAP"
BASE COUNT          297 a          553 c          636 g          256 t
ORIGIN      
        1 ggcatcggcg agcgccggcg tctgcgcgac gttgaagtaa tagaggaggc cgatccagaa
       61 cacgccggcg acgatatgga accagcgcgc caggccgagc ggatagaagg tgacgttcag
      121 tccggtgatc accagcgcga gcaccaccgc gatcacaatg ccgaggacca gggactgccg
      181 atggttgttc agggggttca ttgcttcggt ctcccaagtt attgtttcga ttgacctggg
      241 ccactttgga gcgtttgaag aataggcgag gcacccccat aattcaacgc atgcggaacc
      301 gagcgtgact gaggcgcccg tacgcgcgac gccgccggag cgtccgccat caccgtgcat
      361 caatgtttgc tccctggaca gtcggggata ctgcatcgga tgcctgcgaa cgggggcgga
      421 gatcggacga tggctcgcca tgagctctga ggagcagtgg cgcctgatcg cggagctcga
      481 gaagcgccgc aggcaactag aggtagttac gatggatgtc gtcgagagaa tcaagtcgga
      541 gctggccgcg ggcccggtgg tgctgttcat gaagggcacg ccggatttcc cgcagtgcgg
      601 attctccgcg cagacggtgg cggcgctgcg cgccgtcggc gccaacttcc gctacgtgaa
      661 catcttcgag gagccggaga ttcgcgaggc gctcaagcgc tactccaact ggccgactta
      721 cccgcagctg tacgtgaagg gcgagctgat cggcggctgc gacatcgcgc tcgagatgtt
      781 gaagagcggc gagctgaaga aactcatgag cgaggcgggg gcggcgcagt aatcgcctcc
      841 tcgagcggcg catcctcacc cgccgcatcg ccggccggcg cgtcgagcca gccgagcgcc
      901 gccgcgcgct cgcgcgcgct cgcgaagctc tcccgcagcc ggttgcagac caggcagaag
      961 acctcggcgc agcgctcggc gtcgtagcgc gcgttgtgcg cgctgccggc atcccactcg
     1021 agtcccgcga cggcgagcgc cttggcgagc acggtctggc cgagcgccgc tcccgcgagc
     1081 gtcgccgtgt cgaagcacga gaagggatga aaggggttgc gcttcacgtc ggcgcgcgcc
     1141 accgcggcgt tgaggaaggc caggtcgaag gcggcgttgt ggccgacgag gatcgcgcgg
     1201 cggcagcccg aggcgcgcac cgcatgacgc acctcgcgga agatccgctg cagcgcgtcg
     1261 cgctccggga tcgccggacg cagcgggtga tgcggatcga tgccggtcac cgagagcgac
     1321 gtcgggtcga ggcgcgcacc ttcgaagggc tgcacgtgat agtggtgcgt ggcgccgcgg
     1381 cgcagagtgc cgtcggcgtc gatgtcgatg agcaccgcgg cgatctcgag cagcgcatcg
     1441 gttgccgcat gaaatccgcc gctttccacg tcgatgacca ccggcaggta gccgcggaaa
     1501 cggccggcca tgagctcggc gccggtgcgc ggctcgggcg gaggactcgt cccggcctcg
     1561 ctcatggcgc tggccgcgtg gccgcggcgg ccgctgccag gcgccagggg atgcgcgcgc
     1621 cggcacgcag cggcacgagc tcgtccgcgc cgaatggata gtgctgcggt acctcccagg
     1681 gctcgcgcac cagagtgatc gtcgcggcat tgcgcggcag gcggtagaag tcggcgccat
     1741 ac
//
LOCUS       DATLKL010000880         2457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_180034, whole genome shotgun sequence.
ACCESSION   DATLKL010000880 DATLKL010000000
VERSION     DATLKL010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180034"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VM713_11955"
     CDS             complement(<1..317)
                     /locus_tag="VM713_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85016.1"
                     /translation="MKQLLPRLWCLLALVPLGAAQAAGAPPHYRITHQVALPGDEGWD
                     YLIYDQAGHRLFVAHGSRVLVVDGDKLTVVGEIPETPGVHGIALAPEFNRGYVSAGRA
                     GSVV"
     gene            complement(382..1827)
                     /gene="nuoN"
                     /locus_tag="VM713_11960"
     CDS             complement(382..1827)
                     /gene="nuoN"
                     /locus_tag="VM713_11960"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HVH85017.1"
                     /translation="MPANPMSLSSLAPAIAEIWLVAAICVVLLVDVFAGERRRGLTPT
                     LTLLALALGAALTVQYGEVARRTVLFDGLYVADELGWALKLAAFLIVAVALLYSRTYL
                     ENRSILRGEYYVLALTALLGIFVLVSANSLLTLYIGVELLALSVYSMVAFDRDSGVSA
                     ESAMKYFVLGAIASGALLYGMSLIYGLTGTLNLGEIAARLQGPARPGLILGLTFIVVA
                     VAFKLGAVPFHMWLPDVYEGAPTSVTLFIGTAPKIAYFALALRLLAYGLGGAVGEWSQ
                     MLAALAVLTLVVGNVVAIVQTNLKRMLAYSTIANVGFIVLGFVAGTPEGYSAALYYTL
                     VYVLVALGSFGVILLAASKGFEADRLDDYKGLYQRDPMLALAMMVLMFSTAGVPPFVG
                     FWAKLRIFQVLWETNHLWLVVLAAATSVVGAYYYLRVIKLMYFDPPAEALPPTARSAG
                     VRFALGLNALAVLVLGLLPGPLLDLCARLIH"
     gene            complement(1832..>2457)
                     /locus_tag="VM713_11965"
     CDS             complement(1832..>2457)
                     /locus_tag="VM713_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVH85018.1"
                     /translation="DMKKLIAYSSIAHMGFVTLGAFVIYEIVRATGSTRGAGMGLDGA
                     MVQMISHGLISGALFLCVGVLYDRVHSRQIADYGGVVNTMPVFATFMVLFALANTGLP
                     GTSGFVGEFLVIIASFRASFWYAALAALTLILGACYTLWLVKRVIFGAVATPKVAALE
                     DLNPREFVVLGALAVAVLLVGVWPAPLLKVMQPTIHHLLTQAIATKL"
BASE COUNT          478 a          799 c          860 g          320 t
ORIGIN      
        1 acgacgctgc cagcgcgtcc cgcgctcaca tagccgcgat tgaactctgg cgcgagcgcg
       61 atgccgtgca cgccgggcgt ttccggaatc tccccgacga ccgtcagctt gtctccgtcg
      121 accaccagga cgcgcgagcc atgcgcgacg aacaggcggt ggccggcctg atcgtagatc
      181 aggtagtccc agccctcgtc cccgggaagc gcgacctggt gagtgatgcg gtagtgaggc
      241 ggcgcgcccg ccgcctgtgc agcgccaagc ggcacgagag cgaggaggca ccatagccgg
      301 ggaagcagct gtttcatgag ggggtctccg tggtcatggc gggaacggcg cagcctcctg
      361 ccgcgcggcg gcgctgcgca gtcagtgaat cagccgtgcg cacaggtcga gcagcggacc
      421 cggcaggagg ccgagcacga gtacggcgag cgcattgagg ccgagtgcga agcgcacgcc
      481 ggcggatctg gccgtcgggg gcagggcctc ggcgggcgga tcgaagtaca tgagcttgat
      541 cacgcgcagg taatagtagg cacccacgac cgacgtggcg gctgcaagca ccaccagcca
      601 caggtggttg gtctcccaaa gtacctggaa gatacgcagc ttggcccaga agcccacgaa
      661 cggcggcacg ccggcggtcg agaacatgag caccatcatc gcgagcgcca gcatcggatc
      721 gcgctgatac aggcccttgt agtcgtcgag gcggtcggcc tcgaagccct tggaggccgc
      781 gagcaggatg acaccgaagg agccgagcgc tacgagcacg tacaccagtg tgtagtagag
      841 cgcggcgctg tagccttcgg gcgtgccggc gacgaagccg agcacgatga agccgacgtt
      901 ggcgatggtg gagtatgcca gcatgcgctt caggttcgtc tgcacgatcg ccaccacgtt
      961 gcccaccacg agcgtgagca ccgcgagcgc ggccagcatc tgcgaccatt cgcccaccgc
     1021 cccaccgagc ccgtaagcca gcaggcgcag cgccagggcg aaatacgcga tcttcggggc
     1081 ggtgccgatg aataaagtca cgctggtcgg cgcgccctcg tagacatcgg gcagccacat
     1141 gtggaagggc acggcgccga gcttgaaggc gacggccacc acgatgaagg tgagcccgag
     1201 aatgaggccc ggacgggccg gcccctgcag ccgcgccgcg atctcgccca ggttcagcgt
     1261 gccggtgagg ccgtagatga gcgacatgcc gtagagcagc gcccctgagg cgatcgcgcc
     1321 cagcacgaag tatttcatgg cggactcggc cgacacgccg gagtcgcggt caaaggccac
     1381 catcgagtac accgagagcg cgagcagctc cacgccgata tagagcgtca gcaggctgtt
     1441 agccgagacc agcacgaaga tgccgagcag cgccgtgagc gcgagcacgt agtactcgcc
     1501 gcgcaggatg ctgcggttct cgaggtaggt gcgcgaatag agcagcgcca cggcgacgat
     1561 caggaaggcc gccagcttca gcgcccagcc gagctcgtcg gcgacgtaca ggccgtcgaa
     1621 cagcaccgtg cgccgtgcga cctcgccgta ctgcaccgtg agcgcggcgc ccagcgccag
     1681 cgcgagcagc gtaagcgtcg gggtcagtcc gcgccggcgc tcgcccgcga acacgtcgac
     1741 gagcagcacg acgcagatcg cggcgacgag ccagatctcc gcgatcgccg gcgccaggct
     1801 gctgagactc atcgggttcg cgggcatggc ttcacagctt ggtagcgatc gcctgggtga
     1861 ggaggtggtg gatcgtaggc tgcatcacct tgagaagcgg tgcgggccac accccgacca
     1921 ggagcaccgc aacggcaagc gccccgagca ccacgaactc acgcgggttc aggtcctcga
     1981 gcgcggcgac cttgggtgtg gcgacggcgc cgaagatgac gcgcttcacc agccacagcg
     2041 tgtagcaggc gccgagaatc agcgtgagcg cggcgagcgc ggcgtaccag aagctcgccc
     2101 tgaagctcgc gatgatcacc aggaattcgc cgacgaaccc ggaggtgccg gggagccccg
     2161 tgttggcgag ggcgaacagc accatgaagg tggcgaatac cggcatggtg ttgaccacgc
     2221 cgccgtagtc ggcgatctgg cggctatgca cccggtcgta gagcacgccg acgcacagga
     2281 acagcgcccc ggagatgagc ccgtgggaga tcatctgcac cattgcgccg tccagcccca
     2341 tgccggcgcc gcgggtcgag ccggtcgcgc gcacgatctc gtagatcacg aaggcgccga
     2401 gcgtcacgaa gcccatgtgc gcgatcgagg agtaggcaat gagcttcttc atgtcct
//
LOCUS       DATLKL010000881         2393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_44723, whole genome shotgun sequence.
ACCESSION   DATLKL010000881 DATLKL010000000
VERSION     DATLKL010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44723"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1240)
                     /locus_tag="VM713_11970"
     CDS             complement(38..1240)
                     /locus_tag="VM713_11970"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVH85019.1"
                     /translation="MDGIGETGLWPADRPNVGLVLTGGGARSAYQVGVLLALAELLPR
                     ARNPFQVIVGTSAGAVAASVLAAEAHRWRRAVAGLERVWANFRSGQVFHVDAPHMLRA
                     GAHWVLALMSGGLVLAPPKSILDNTPLRELLARHVDCAGVRRSIARGHLRAFALCATS
                     YVTGQSVAFFDGVESIRDWSRAQRVGRRCEFNLDHLMASVAIPLLFPPMKIDDEYFGD
                     GAMRQLNPLSPAIHLGADRLLIIGVRARRAAGVTVDRLKPIMPTPGEIFGYMLDTLFT
                     DQIYGDLEQLERINTLVESAPDAARGERHVETLMLAPSVDPREIAARHIAEIPRGLRA
                     LLRVIGGRDASGSQLASYLIFEAGYTRALIELGYRDAMEARSALVAFMSGARLPAVLT
                     AAGVAQAT"
     gene            complement(1358..>2393)
                     /locus_tag="VM713_11975"
     CDS             complement(1358..>2393)
                     /locus_tag="VM713_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVH85020.1"
                     /translation="DGHIGWSIYGRIPDGTGPARALGKIQWTTAANHPRLIDPPRGRI
                     WTANARASVDERELTLIGGADSPYGANYVLGARAQQIRGDLLALSAQEQPADMLHVQL
                     DDRALFLARWRELLLRLIDVTSSSAHPPRAEFRRLVEAWDARAGVDSVGYRLVRSFRE
                     HTQQAVWDMLLAALAVPPDYDVGAPFQFESPLWQMVTRQPLHLLAREYADWPAFLLAQ
                     LDATIADLGRECAPLAQCTWGKHNVVRIRHPLARALPALARLLDMPPVELPGDADMPR
                     VQKVAFGASERFAVTPGREQEGYFELPGGQSGHPLSPYYRSGFLAWAHGEPQPFLPGA
                     AQHTFTLKPN"
BASE COUNT          365 a          825 c          873 g          330 t
ORIGIN      
        1 cgcgtctgtg aacagcatgc cgcagattgt cgaagtctca ggtcgcctgc gcaaccccgg
       61 ctgcggtcag caccgccggc agccgcgcgc cgctcatgaa ggcgaccagc gccgagcgcg
      121 cctccatggc gtcgcgatag ccgagctcga tgagcgcccg cgtgtagccg gcctcgaaga
      181 tcaggtagct cgcgagctgc gagccggagg cgtcacgtcc gccgatcacc cgcagcagcg
      241 cccgcagccc gcgcggtatc tcggcgatgt gccgtgcggc gatctcgcgc ggatcgacgc
      301 tcggggcaag catcagcgtc tcgacgtggc gctcgccacg ggcggcatcg ggtgcgctct
      361 ccaccagggt gttgatacgc tcgagctgct cgaggtcgcc gtagatctgg tccgtgaaca
      421 gggtgtcgag catgtagccg aagatctcgc cgggggtcgg catgatgggc ttgaggcggt
      481 cgaccgtgac gccggcggcg cgccgggcgc gtacgccgat gatcagcagc cggtcggcgc
      541 ccaggtgaat cgccgggctc aggggattga gctggcgcat ggcgccgtcg ccgaagtact
      601 cgtcgtcgat cttcatcggc gggaacagca gcggaatcgc cacgctcgcc atcaggtgat
      661 cgaggttgaa ctcgcagcgg cggccgacgc gctgtgcgcg cgaccagtcg cggatcgact
      721 cgacgccgtc gaagaaggcc accgactggc cggtcacgta gctggtggcg cacagggcga
      781 aggcgcgcag gtggccgcgg gcgatgctgc gccgtactcc ggcgcagtcg acgtgacggg
      841 cgaggagctc acgcagcggc gtgttgtcga ggatcgactt ggggggcgcg agcaccagcc
      901 cgccggacat gagggcgagc acccagtgcg cgccggcacg cagcatgtgc ggcgcgtcga
      961 catgaaagac ctgcccggag cggaagttcg cccagacgcg ctcgagtccc gccaccgcac
     1021 ggcgccagcg atgtgcctcg gctgccagca cgctcgccgc gaccgctccc gccgaggtgc
     1081 ccacgatgac ctgaaacggg ttgcgggcgc gcggcaacaa ctcggcgagg gcgagcagca
     1141 cgcccacctg atacgccgag cgcgccccgc cgcccgtcag caccagcccg acgtttggcc
     1201 ggtcagccgg ccagagcccg gtttcgccga tgccgtccat tgatgatcct atgccactcc
     1261 tattatgaaa cctgccacgc tgaaccccgt gtgaccggcg tcgcgcgcct cgcgtcgccc
     1321 ggcgtcgacg gcgtctcgcc gcgagcctag ccctgggcta gttgggcttc agggtgaagg
     1381 tgtgctgcgc cgctcccgga aggaaaggtt gcggctcgcc atgcgcccag gcgagaaacc
     1441 ccgaccggta atagggcgac agcgggtggc cgctttgccc gcccggcagc tcgaagtaac
     1501 cctcctgctc gcggcccgga gtcacggcga agcgctccga tgcgccgaag gcgaccttct
     1561 gtacgcgcgg catgtcggca tcgcccggga gctccacagg cggcatgtcg aggaggcgcg
     1621 cgagcgccgg cagcgcgcgc gcgagcggat ggcggatgcg caccacgttg tgcttccccc
     1681 acgtgcactg ggccagcggc gcgcattccc tgccgagatc tgcgatggtg gcatcgagct
     1741 gagccaggag gaacgccggc cagtcggcgt actcccgggc gaggaggtgc agcggctggc
     1801 gggtcaccat ctgccagagc gggctctcga actgaaaggg cgccccgacg tcgtagtcgg
     1861 gcggcaccgc gagcgcggcg agcagcatgt cccagacggc ctgctgcgtg tgctcgcgga
     1921 agctgcgcac cagccgatag cccaccgagt cgacgccggc gcgcgcgtcc caggcctcga
     1981 cgagccggcg gaactcggcg cgcgggggat gcgcgctcga gctcgtcaca tcaatcaggc
     2041 gcagcagcag ctcccgccag cgcgcgagga acagggcgcg gtcgtcgagc tgcacgtgca
     2101 gcatgtcggc aggctgctcc tgagcgctga gcgcgagcag gtcaccccgg atctgctggg
     2161 cgcgcgcgcc gagtacgtag ttcgcgccgt agggcgagtc cgccccgccg atgagtgtga
     2221 gttcccgctc gtcgacgctc gcccgcgcat tggcggtcca gatgcgcccg cgcggcggat
     2281 cgatgaggcg cggatgattc gccgcggtgg tccactggat cttgccgagg gcgcgcgccg
     2341 ggccggtccc gtccgggatg cggccgtaaa tgctccagcc gatgtggccg tcg
//
LOCUS       DATLKL010000882         4112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_168896, whole genome shotgun sequence.
ACCESSION   DATLKL010000882 DATLKL010000000
VERSION     DATLKL010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168896"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(615..1454)
                     /locus_tag="VM713_11980"
     CDS             complement(615..1454)
                     /locus_tag="VM713_11980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVH85021.1"
                     /translation="MCDDEQINDMIEYQLKSPQLSRRRFGAFTLGAGVASLLPPGAGA
                     AWIAETDVEIKTPDGTADAYFVHPSQGVYPGILMWPDIYGLRPAFRQMGRRLAEAGYS
                     VLVVNPFYRAKRAPVVPEHADYNDPATRETLMALVATLNPDAIVSDSKAYVAWLDSQP
                     SVDRKRRMGTLGYCMSGPATLRAAAALPERIGAGASFHGGGLVTDKPDSPHLLIPKIK
                     AHYLFAIAENDDKRQPEAKTVLRDAFAKANMPAEIEVYAGTLHGWCPVDARVYNDEQA
                     DRA"
     gene            1512..2948
                     /locus_tag="VM713_11985"
     CDS             1512..2948
                     /locus_tag="VM713_11985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVH85022.1"
                     /translation="MSVAEAAPLPLASRRLLPWLVAVAFFMQSLDTTILNTAVPGIAR
                     ALGVAPLSMKAVLATYTLSLAVFIPVSGWMADRFGTRRVFAAAIGLFTAGSLLCGLAR
                     NIHLLVVFRMLQGCGGAMMVPVGRLTLVRTFGKSELVRAMSFVAVPGLLGPLFGPLAG
                     GLTTGLAHWSVIFFINVPIGVAGLYLVERYLPDYREAHTAPLDLGGFVLFGSGIALLS
                     YVLEVFGEHRLSGAVIAALLALSLALLAGYGLHAARALHPLLDLALFRIRTFRAAVSG
                     GFFTRISISGIPFLLPLLLQVGLGYTPIQAGLLMMSQAFAAMGLKVIMPRILARFGYR
                     AVLVSNTVMLGVLILLFATIGPATPVWLIVLQVSAFGLFSSIQYTSMNTLVYADVSGA
                     AASDASTIASTGQQLSISFAVAVASLVTALFVPDRFHTAPGELLHGIHEALVVLGAAT
                     IVSTLVFRSLERDDGSAVSRHREARDAA"
     gene            complement(2956..>4112)
                     /locus_tag="VM713_11990"
     CDS             complement(2956..>4112)
                     /locus_tag="VM713_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85023.1"
                     /translation="EPLPAGNGRDIAASKCVACHDAGRLAYPGYTREGWRKVIDRMTK
                     LGATVSPEQVPLLSDYLARAFPERPPPAARIIPGTARASFKEWAVATPGAFPHDPLAT
                     ADGAIWYTGQRASLLGRIDPGSGAIREYPTSIPDSGPHGLVADAAGNIWFTANYAGYI
                     GKLDPATGVITVYRMPDERARDPHTAIFDQHGVLWFTVQGANFVGQLLPQSGTVRLVE
                     LPAAHSLPYGIVVSSRGVPFFAEFGANRIGRIDPDTLAIHEYVLPNAGSRPRRLGITA
                     DDAIWYTDYARGYIGRLDPGSGATREWASPGGAVSLPYGITALNEVIWYSESGVRPNT
                     LVRFDPRTEGFQSWAIPSGGGVVRNMMPTLDGRLVLAESGVGKLARVDVQ"
BASE COUNT          586 a         1445 c         1356 g          725 t
ORIGIN      
        1 ctcacgacat aacgccgccc ttcggcgccg gaagccgctg accgcgaatc cgcgccccgc
       61 cggctacgcc gccttagcgg cgcgctcagc caaagcgcca gttatgcgcc accgctcgca
      121 gcaccgggct cctgacggag agatcccgcg ctctcgcccc cgcgcttaag gttcttatgg
      181 cgctgaacga tgttgcgatg cggagtctct catcgccgac acattgacct acgaaggtgg
      241 agcgctcagc gtcctgacat gactcgagtg ttgcgtggag accagaccgg cgtaggcgac
      301 acactcgtcg cctgtgccaa gtcgctcgcg cgaggctatc ccaagtggta ctgacagcgg
      361 tgggattcct gcttctcttc ctctccgttg gggcactctg cctgttctgt tggttgagct
      421 gcgcatgcct gggggcggcg cgttcgccga agccgcccgc tcaggacaac tccctcgagc
      481 atgcggccga tgacatcacg ccggccgtcg gcgccggcag ccgctgaccc cgagagttcg
      541 cgcccgccgg ctctctaggc ggccttcgcc gcgtgcggtg ccagcgccgt gctgaaaagc
      601 gcaagcaacc ggctctaggc cctatccgcc tgctcatcgt tgtagacgcg cgcgtccacc
      661 gggcaccagc cgtgcagcgt cccggcatac acctcgatct cggccggcat gttcgccttg
      721 gcaaacgcat cccggagcac ggtcttcgcc tcgggctggc gcttgtcgtc gttctcggcg
      781 atcgcgaaca ggtagtgcgc cttgatcttc gggatcagga ggtgcgggct gtcgggcttg
      841 tcggtcacga gcccgccgcc gtggaatgaa gctccggcac cgatgcgttc gggaagcgcc
      901 gccgccgcgc gcagggtagc cgggccgctc atgcagtagc cgagcgtgcc catcctgcgc
      961 ttgcgatcga cggagggctg ggaatcgagc caggccacgt aggccttcga atccgacacg
     1021 atcgcatcag ggttgagcgt ggccacgagc gccatgaggg tctcgcgcgt tgccgggtcg
     1081 ttgtagtcgg cgtgctccgg cacgaccggc gctcgcttgg cccggtagaa ggggttcacg
     1141 accagtaccg aataacctgc ctccgcgagc cggcgcccca tctgccggaa ggcgggacgc
     1201 aagccgtaaa tgtccggcca catgagtatg ccgggataca ccccctgaga cggatggacg
     1261 aagtacgcgt ccgcggtgcc gtcgggagtc ttgatctcga cgtcggtctc cgcgatccag
     1321 gccgcgccgg cacccggcgg caggagtgac gcgacgcccg caccgagggt gaaggctccg
     1381 aatcgccgcc gcgacagttg tggcgacttc agctgatact cgatcatgtc gttgatctgc
     1441 tcgtcgtcgc acatagctgc tctccgcacg cggcacgggg cgcgcagtat atcgcgccat
     1501 gatgtccgcc catgtcagta gctgaagccg caccgctccc gcttgcctcc cgccggctcc
     1561 tgccctggct ggtcgccgtg gcattcttca tgcagtcgct cgacacgacc atcctcaaca
     1621 ccgcggtgcc gggcatcgcg cgcgccctcg gcgtcgcgcc gctcagcatg aaggcggtgc
     1681 tcgccaccta caccctgagt ctcgcggtgt tcattccggt gagcggctgg atggcggacc
     1741 gcttcggaac gcggcgcgtg ttcgcggcgg ccatcggtct ctttaccgcc gggtcgctgc
     1801 tgtgcgggct ggcgcgcaac atccatctgc tggtcgtgtt ccgcatgctg cagggctgcg
     1861 gcggcgccat gatggtgccg gtcggcaggc tcacgctggt gcgcaccttc ggcaaatccg
     1921 agctggtgcg tgccatgagc ttcgtcgcgg tcccgggcct gctcgggccg ctgtttggcc
     1981 cgctcgccgg ggggctgacc accgggctgg cgcattggag cgtgatcttc ttcatcaacg
     2041 ttccgatcgg cgtcgctggg ctctacctcg tcgagcgcta tctgcccgac taccgcgagg
     2101 cacacacggc gccgctcgac ctcgggggct ttgtgctgtt cggctccggg atcgcgctgc
     2161 tctcctacgt cctcgaggtg ttcggcgagc atcgcctgag cggcgccgtg attgccgcgc
     2221 tcctcgcgct gtcgctcgcc ctgctcgccg gctatggtct gcacgccgcg cgcgcgctgc
     2281 acccgctgct ggacctcgcg ctgttccgca ttcgcacctt ccgggctgcg gtgagcggcg
     2341 ggttcttcac acgcatcagc atcagcggca tcccgttcct gctgccgctg ctgctgcagg
     2401 tgggtctcgg ctacacgccg atccaggcgg gtctgctcat gatgtcgcag gccttcgcag
     2461 ccatgggcct caaggtgatc atgccgcgga tactcgcgcg cttcggctat cgcgcagtcc
     2521 tcgtctccaa cacggtcatg ctgggagtcc tgatcctgct gttcgccacc atcggaccgg
     2581 cgacccccgt gtggctcatc gtgctgcagg tgtccgcctt cggactgttc tcctcgatcc
     2641 agtacaccag catgaacaca ctggtgtatg ccgacgtgag cggcgcggcg gcgagcgatg
     2701 ccagcaccat cgccagcacc ggccagcagc tctcgatcag cttcgcggtc gccgtggcat
     2761 cgctcgtgac cgcgctgttc gttcccgacc gcttccacac ggcgcccggg gagctgctgc
     2821 acggcatcca cgaggccctg gtcgtgctcg gcgccgcgac tatcgtctcg acgctcgtgt
     2881 tccgcagcct cgagcgcgat gacggcagcg ccgtgagccg ccaccgcgag gcgcgcgacg
     2941 cagcctgagc gcggcctact gcacgtccac ccgtgcgagc tttcccactc cgctctcggc
     3001 aagcaccagg cggccgtcga gtgttggcat catgttgcgt accacgcccc cgcccgaggg
     3061 aatcgcccac gactggaatc cctccgtgcg agggtcgaag cgcaccagcg tgttggggcg
     3121 cacgcccgat tcgctgtacc agatgacctc gttcagcgcg gtgatgccat aggggagcga
     3181 gaccgcaccg ccgggcgagg cccattcgcg ggtcgcaccg ctccctgggt cgaggcggcc
     3241 gatgtagccg cgcgcgtagt ccgtgtacca gatcgcatcg tcggcggtga tgcccaagcg
     3301 gcggggccgc gagccggcgt ttggcaggac gtactcatgt atcgccagag tgtcaggatc
     3361 gatgcgcccg atgcggttgg cgccgaattc ggcaaagaac ggcacgcccc gcgagctcac
     3421 tacgatccca taaggcaagg agtgggcggc cggcagctcc accaggcgca ccgtgccgct
     3481 ctgcggcagc agctgcccga cgaagttcgc tccctgcacc gtgaaccaca gcaccccgtg
     3541 ctgatcaaaa atcgccgtgt gcggatcgcg cgcccgctcg tccggcatcc ggtagacggt
     3601 gatcacgccg gtcgccggat cgagcttacc gatgtagccg gcgtagttgg cggtgaacca
     3661 aatgttaccg gcggcgtccg ccaccagccc gtggggccct gagtccggga tgctcgtcgg
     3721 atactccctg atcgcccccg acccagggtc gatgcgtccg aggaggctcg cacgctgccc
     3781 ggtgtaccag atcgccccgt ccgcggtggc gagcggatcg tgaggaaacg ctccgggagt
     3841 cgccaccgcc cactccttga agctggcgcg ggcagtgccg ggaatgatgc gcgccgccgg
     3901 tggcggccgc tcggggaaag cgcgcgccag ataatccgat agcagcggaa cctgctcggg
     3961 gctaaccgtg gcgccgagtt tcgtcatgcg gtcgatgacc ttccgccacc cctcgcgtgt
     4021 gtagcccggg tacgcgaggc ggcccgcgtc gtgacaggcg acacatttgc tggcggcgat
     4081 gtcccggccg ttcccggcgg gcagcggctc gg
//
LOCUS       DATLKL010000883         1693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_44728, whole genome shotgun sequence.
ACCESSION   DATLKL010000883 DATLKL010000000
VERSION     DATLKL010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44728"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..774
                     /locus_tag="VM713_11995"
     CDS             151..774
                     /locus_tag="VM713_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVH85024.1"
                     /translation="MLEWRGDTRRFNPGVASACLLLCAFSSARADDLLGPYFGATFGK
                     ATVDASTGLVSGFGIHSSAYTIVAGWRPIPELSAELQYLAFGHSAGSSIYKNSNVPLT
                     NSASRKGEGAFGILYLPTPIVDFYLKAGLAKLHTTATTVVGTCPKGGVCPPLANPLPV
                     DSTGVGAAGGGGVMYRFRSLEVRAEYERFAALGGNPYMVTGGLTWTF"
     gene            complement(818..>1693)
                     /locus_tag="VM713_12000"
     CDS             complement(818..>1693)
                     /locus_tag="VM713_12000"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVH85025.1"
                     /translation="VPGADLEGVHYLRTIADADRLRAGFTSGGRLLIVGGGYIGLEVA
                     ATARELGVDVTVLGMAERVMNRVTCPEVSAFYEAEHARHGVRIRCHEQVRALHADART
                     GRVRSVLTEAGGEYPADLVLIGVGVAPADELARAAGLDCENGVVTEAHCRTSHEGIYA
                     AGDCASHLSRQYGRHLRLESVDNAFEQGTTVALNLMGNETLHDKLPWFWSDQFDLKLV
                     IVGVAQGYDTVVMRGAPAARSFSACYLRGGELVAIDTVNAPKDQMAARKLIAAHARPR
                     PDRLADPAIALRDTV"
BASE COUNT          236 a          603 c          576 g          278 t
ORIGIN      
        1 ggtagatctc aaggacctgc tgatggtgtt gcgctaggac gcgcacgtgc gcgctcaccg
       61 acgagtgtcg gcgcgtggag tcatccgtcg gtaccaacac ccttgctgta tgctctctcc
      121 gcagggagta cctggtgcgg aggcgcgtcg atgctcgagt ggcgtggcga tacgcgacga
      181 ttcaaccctg gcgtagcttc cgcctgtctc ttgctctgcg cgttctccag cgcgcgcgcg
      241 gacgacctgc tcggtcccta tttcggcgcc accttcggca aggcgacggt cgacgcctcg
      301 accgggctcg tcagcggctt cggcatccac agctctgcct acacgatcgt cgccggctgg
      361 cgtccgatcc cggagctgtc ggcggagctt cagtacctcg ccttcggcca ttcggccggg
      421 agctcgatct ataagaactc caacgtcccg ctgacgaaca gcgcgagccg caagggcgag
      481 ggagcgttcg gcattctcta tctgccgacg ccaatcgtcg atttctacct gaaggctggg
      541 ctcgccaagc tccataccac ggcgaccacg gtcgtcggca cctgcccgaa gggcggcgtg
      601 tgtccgccgc tcgccaatcc tctgccggtc gacagcaccg gcgtcggggc tgccgggggc
      661 ggcggcgtga tgtaccgctt ccgctcgctc gaggtgcgcg ccgagtacga gcgcttcgca
      721 gcgctcggcg gcaatcccta catggtgacg ggcggcctca cctggacctt ctgagtgccg
      781 ccggagcgcc gcgcggtcag cgcgcttcag cgcgtggtca caccgtgtcc ctgagcgcga
      841 tcgccggatc cgcgagccgg tccgggcggg gccgggcgtg cgccgcgatc agcttgcgcg
      901 ccgccatctg atccttcggc gcgttgacgg tgtcgatggc gacgagttcc ccgccgcgca
      961 gatagcaggc gctgaagctg cgcgccgccg gcgcgccgcg catcaccacg gtgtcgtagc
     1021 cctgcgcgac gccgacgatc accagcttca ggtcgaactg gtccgaccag aaccacggca
     1081 gcttgtcgtg cagtgtctcg ttgcccatca ggttgagggc gacggtcgtc ccctgctcga
     1141 aggcgttgtc gaccgactcg agacgcaggt gccggccgta ctggcggctc aggtgactcg
     1201 cgcagtcgcc ggcagcgtag attccctcgt gcgaggttcg gcagtgcgcc tcggtcacga
     1261 ccccgttctc gcagtcgagg ccggccgccc gcgcgagctc gtcggcgggc gccacgccga
     1321 caccgatcag cacgaggtcc gcggggtact cgccgcctgc ttcggtgagg acgctgcgaa
     1381 cgcgcccggt gcgcgcatct gcgtgcagcg cccgcacctg ctcgtggcag cggatgcgca
     1441 cgccgtggcg cgcgtgctcc gcctcgtaga acgcggagac ctcggggcag gtgacgcgat
     1501 tcatgacgcg ctcggccatc ccgagcaccg tcacatccac gccgagctcg cgcgcggtgg
     1561 ccgcgacctc gagcccgatg tagccgccgc cgacgatcag cagccgcccg ccgctcgtga
     1621 agccagcgcg cagacggtca gcgtcggcga tggtgcgcag gtaatgcaca ccctcgaggt
     1681 cggcgccggg cac
//
LOCUS       DATLKL010000884         2388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_10687, whole genome shotgun sequence.
ACCESSION   DATLKL010000884 DATLKL010000000
VERSION     DATLKL010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10687"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VM713_12005"
     CDS             <1..926
                     /locus_tag="VM713_12005"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HVH85026.1"
                     /translation="RSGPDRRASGGASRALPVPPCALEAGVNGQMITLYDRGGHIPLL
                     WTHLIPATLEGRAVHNVQNAMFAAALAFSLGIKLDAIRQGLRTFDSTFFQAPGRMNVF
                     NEHPFKVLFDYGHNAHAVGVMTDLAQRLDVQGRRIVVLAGPGDRRDEDLIAIARAAAG
                     RFDHYICRRDDGLRDRAADEVPRIQAAALREAGVAAEAISIIPDEQEAIEAALNLGQQ
                     GDLLLIFADQLTRSWKQITKFKPAGGGARTEPREARAGPAAARGEDGHFVAGFGADGA
                     GRGHEAGPPAASLEGLIRDERGVRFAPEADD"
     gene            1004..>2388
                     /locus_tag="VM713_12010"
     CDS             1004..>2388
                     /locus_tag="VM713_12010"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HVH85027.1"
                     /translation="MLETVGLEPDAALIEGWHTRVTRARRRLGWTMPGIVARRHAGGV
                     SLALAAPVDQLFTATEVNEWALCATLLARDPACWNDLERALAAAAPETAADGSATVAP
                     VLAEDAALERLALLAAREARPELIRLIAAADAHSLPYVLDETLLTLGAGAGGRDFELT
                     ALPQAAAVPWSDCSDIPTALVTGSNGKTTSVRLLAACAEAHGWQSAYCCTDGVFLGDT
                     ALASGDYSGPLGARRVMRERRARCAIVETARGGILRRGIALSHAHVALVTNVSPDHFG
                     EYGIDDLDGLADVKLSVAGLLGPDGLLVLNADDERLVAKAPELARRFGRAPPLGWFAL
                     EADAALLTEHRARGGATCGVRGGRLLLSQGARQQDLGAVSAMPLSVGGSARYNIANLA
                     GAALAAAALGVPPASIAAVFARFGASVADNFGRMMRFERGGVRILVDYAHNVEGLKGL
                     LEVAERLRGGSG"
BASE COUNT          308 a          860 c          879 g          341 t
ORIGIN      
        1 cccgaagcgg accggatcgc cgcgcgtcgg gtggcgccag tcgcgcgctg ccagtgccgc
       61 cctgcgcgct cgaggccggt gtcaacggcc agatgatcac gctctacgac cgcggcgggc
      121 acataccgct cctctggacc cacctgattc ccgccaccct cgagggccgc gccgtccaca
      181 acgtgcagaa tgcgatgttc gctgcggcgc tggcgttctc actcggcatc aagctggatg
      241 cgatccgcca ggggctgcgc accttcgact cgacgttctt ccaggcgccg ggccgcatga
      301 acgtgttcaa cgagcatccc ttcaaggtgc tgttcgacta cgggcacaac gcgcacgccg
      361 tcggcgtgat gaccgatctc gctcagcgcc tcgacgtgca gggcaggcgc atcgtggtgc
      421 tcgccggccc cggcgaccga cgcgacgagg acctgatcgc catcgcgcgc gccgctgccg
      481 gccgtttcga tcactacatc tgccgccgcg acgacggcct gcgcgatcgc gccgcggacg
      541 aggtgccgcg catccaggcg gcggcgctgc gcgaggccgg cgtggcggcg gaagccatct
      601 cgatcatccc cgacgagcag gaggcgatcg aggctgcttt gaacctcggg cagcaggggg
      661 acctgctgtt gatcttcgcc gaccagctca cgcgctcgtg gaagcagatc acgaagttca
      721 agcccgcggg aggcggcgca cgcacggagc cgcgcgaggc gcgggccggg ccggccgcgg
      781 cgcgcggcga ggatggccac ttcgtcgcgg gcttcggcgc cgatggcgct gggcgcgggc
      841 acgaggccgg acctcccgcg gcgagcctcg agggtctgat ccgtgatgag cggggagtgc
      901 ggttcgctcc ggaggccgac gactgaacgc gcccccagcg ctgccgttcg acgcttcgcg
      961 gcgtctcacc ggcgcgaatc tgttcttcgc ctgcgccggc gcggtgcttg agaccgtcgg
     1021 cctcgagccc gatgcggcgc tcatcgaggg ctggcacacg cgggtcacgc gggcccggcg
     1081 gcgcctcggc tggaccatgc cgggtatcgt cgcgcggcgg cacgcgggcg gcgtctcgct
     1141 cgcgctcgcc gcccccgttg atcagctctt taccgctacc gaggtcaacg aatgggccct
     1201 gtgcgcgacg ctcctggcgc gcgatccggc gtgctggaac gatctcgaac gcgcgctcgc
     1261 agcagcggcg cccgagacag cggccgatgg cagcgcgacc gtcgcgccgg tgctggcgga
     1321 ggatgcggcg ctcgagcgcc tggcgctgct cgccgcccgc gaggcgcggc cggagctcat
     1381 ccggctgatc gcggccgccg acgcgcattc gctgccttac gtcctcgatg agacgctgct
     1441 cactctcggc gccggcgccg gcgggcgcga cttcgagctc accgcgctcc cgcaagcagc
     1501 cgccgtgccg tggagcgact gcagcgacat tccgacggcg ctcgtcaccg gctcaaacgg
     1561 caagaccacc tcggtgcggc tgctcgctgc ctgcgcggag gcgcacggct ggcagagcgc
     1621 ctattgctgc accgacggcg tgttcctcgg cgacacggcg ctcgcgagcg gcgactactc
     1681 aggaccgctg ggcgcgcgcc gcgtgatgcg cgagcggcgc gcccggtgtg ccatcgtcga
     1741 gacggcgcgc ggcgggatcc tgcgccgcgg aattgcgctc agccacgcgc acgtcgcgct
     1801 cgtcaccaac gtgagtcccg accatttcgg cgagtacggc atcgacgatc tcgacgggct
     1861 cgccgacgtc aagctctcgg tggccggcct cctcgggccg gacggactcc tggtgttgaa
     1921 tgccgatgac gagcggctgg tggcgaaggc gccggagctg gcgcgtcgct tcggccgtgc
     1981 gccgccgctc ggttggtttg cgctcgaggc cgatgcggcg ctcctcaccg agcatcgcgc
     2041 acgcggcggc gcgacctgcg gggtgcgggg cgggcggctg ctgctcagcc agggcgcaag
     2101 gcagcaagac ctcggcgcgg tgagcgccat gccgctctcc gtcggcggga gcgcgcgcta
     2161 caacatcgcc aatctcgcgg gagctgcgct cgccgcggcg gcgctcggcg tgcccccggc
     2221 gagcatcgcc gcggtgttcg cacgcttcgg cgcgagcgtc gccgacaatt tcgggcgcat
     2281 gatgcgcttc gagcgcggcg gggtgcgcat tctggtcgac tatgcccaca acgtggaggg
     2341 gctgaagggc ctcctcgagg tggcggagcg gctgcgcggc ggcagcgg
//
LOCUS       DATLKL010000885         1759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_44759, whole genome shotgun sequence.
ACCESSION   DATLKL010000885 DATLKL010000000
VERSION     DATLKL010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44759"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VM713_12015"
     CDS             complement(<1..200)
                     /locus_tag="VM713_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85028.1"
                     /translation="MQSSFGFPGFESGAAFGAATARFFELLQSFAAQPQATPGQPPDW
                     TSLSATLARHFEQWLRSSSAGG"
     gene            377..880
                     /gene="phaR"
                     /locus_tag="VM713_12020"
     CDS             377..880
                     /gene="phaR"
                     /locus_tag="VM713_12020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HVH85029.1"
                     /translation="MAEERLIRKYANRRLYDAQDSRHVTVDDLRKLVAAGEHIKVIDD
                     KSGEDLTRSILLQIIASQEQFGTPVLSTQLLEAIIRFYGNPIQQMLTTYLERSIGGLL
                     RQQTVMQAEMAKVLETPMAPLAEMTRQNMEMWAKMQASMLSAFSPGSAAAEADKPATP
                     PPGKRRG"
     gene            956..1717
                     /locus_tag="VM713_12025"
     CDS             956..1717
                     /locus_tag="VM713_12025"
                     /inference="COORDINATES: protein motif:HMM:NF006072.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVH85030.1"
                     /translation="MDVRGKTILVTGAGRGIGRSLSEHFARKGARLALLDTNAEDLED
                     TRARCVQLGAAARSYSADAANEDSVVGALDQVISDFGSLDGLVNNAGIVRDAMLVKVK
                     DGQVVSKMTLAQWQSVIDVNLTGVFLCAREAAQRMIARASGGVIVNISSICRAGNAGQ
                     TNYSAAKAGVMAMTVVWAKELARFGIRVGAVAPGFIRTPMVEGMKPEALARMTAPIPL
                     GRLGEPDEIAHAVGFIFENDLFTGRCVEVDAGLRI"
BASE COUNT          332 a          572 c          571 g          284 t
ORIGIN      
        1 aagcccccag cggacgagga tctgagccac tgctcgaaat gccgtgcgag agtggcgctg
       61 agtgaggtcc agtcaggcgg ctgacccggc gtggcttgcg gctgtgcagc aaaactctgc
      121 agcaactcga aaaaacgcgc cgtggcggcg ccaaaggcgg cgccggactc gaagcccggg
      181 aatccgaaag agctctgcat catcttcccc ccatgtatgc gccgcgcaat acaggtaagt
      241 cctcgccggc agtcggtttc cagatttatg agtttgaccc gaaaaagacg cttccggaat
      301 gccgcccgct cgccccatcg accgtgaagt ccgtcattgt gcgctgcata caccagagtt
      361 agtatccgct tccaccatgg ccgaagagcg gctgatacgc aaatacgcca atcggcgtct
      421 ctacgacgcg caggatagcc gccacgtcac ggtggacgat ctgcgcaagc tcgtggcggc
      481 gggcgagcac atcaaggtca tcgacgacaa gagcggcgag gatctgacgc gcagcatcct
      541 gctgcagatc atcgccagcc aggagcagtt cggtaccccg gtgctgagca cccagctcct
      601 cgaggcgatc atccgtttct acggtaatcc gatccagcag atgctcacca cctacctcga
      661 gcgcagcatc ggcggactcc tgcgacagca gaccgtgatg caggccgaga tggccaaggt
      721 gctcgagacc cccatggcgc cgctcgccga gatgacacgc cagaacatgg agatgtgggc
      781 caagatgcag gcgtcgatgc tctcggcctt ctcccccggc tccgcggctg cggaggccga
      841 caagcccgcc accccaccgc ccggcaagcg ccgcggctga cgttcaggat ccgagggatt
      901 tcgaggtgcg tccgcacccg ccgcagccgt tagacaaacg aaggagtgat cagcagtgga
      961 cgttcgcggt aagacgattc tcgtgaccgg cgccggacgc ggcatcggcc gctcgctctc
     1021 ggagcacttc gcccgcaaag gcgcgcggct cgcgctcctc gacaccaacg ccgaggacct
     1081 cgaagatacg cgggcgcgct gcgtgcagct cggcgctgcc gcgcgcagct acagcgccga
     1141 cgccgccaac gaggacagcg tggtcggcgc cctcgatcag gtcatcagcg acttcggcag
     1201 cctcgacggg ctcgtcaaca acgccggcat cgtgcgtgat gccatgctgg tgaaggtcaa
     1261 ggatggccag gtcgtgagca agatgacgct cgcgcagtgg caatcggtca tcgatgtgaa
     1321 cctcaccggc gtctttctgt gcgcgcgcga ggcggcgcag cgcatgatcg cgcgcgccag
     1381 cggcggggtg atcgtcaaca tctcgagcat ctgccgcgcc ggaaacgccg ggcagaccaa
     1441 ttacagcgcc gccaaggcag gcgtgatggc catgaccgtc gtgtgggcca aggagctcgc
     1501 gcgctttggg atccgggtgg gtgctgtggc tccgggcttc attcgcacac cgatggtcga
     1561 gggcatgaag ccggaggcgt tggcccgcat gaccgcgccg attccgctcg gccgccttgg
     1621 cgagcccgat gagattgcgc acgcggtcgg cttcatcttc gagaacgacc tcttcacggg
     1681 ccgctgtgtg gaggtcgacg cgggtctgcg gatctgactg ctgcggatct gactgctgcg
     1741 gatctgactg ctgtttaag
//
LOCUS       DATLKL010000886         1648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_89941, whole genome shotgun sequence.
ACCESSION   DATLKL010000886 DATLKL010000000
VERSION     DATLKL010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89941"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VM713_12030"
     CDS             <1..271
                     /locus_tag="VM713_12030"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637163.1"
                     /note="Catalyzes the first step in hexosamine metabolism,
                     converting fructose-6P into glucosamine-6P using glutamine
                     as a nitrogen source; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HVH85031.1"
                     /translation="PNNDLLEKLKSNLMEVRARGGELIVFADPEAGIHPSDGVTVIEM
                     PKHVTYVQAPVVYTIPLQLLAYHAAILHGTDVDQPRNLAKSVTVE"
     gene            358..>1648
                     /locus_tag="VM713_12035"
     CDS             358..>1648
                     /locus_tag="VM713_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HVH85032.1"
                     /translation="MSASRAASAADLLAGLSIAGLLLPEAVAYSHLAGLPQQAGVIAL
                     FAGLICYGLIGSSRFAIVSATSSSAAVLASAMLALGATGAAQRAALALLLVVGAGIAF
                     ALCAVLKLGAMSNLIARPVLRGYAFGLALVIAAKQWPTIAGMHTTSGGFFPLIAELVR
                     GLPAWRPPSLYCGIAALLGLFALERVRRVPGALVVICAGILAAPWLASHGVALTGPID
                     LTPSLPQFALPEGTHWPSLAGFSLALMFILYAESYGSIRTYALKHDDAVQPNRDLLAL
                     GVANAVAGFLQGTPVGAGYSATSANEAAGAESRSAGLIAAAAALLLVALFLHWIERIP
                     EPVLAAIVIHAVSKSLRLDVFADYFRWQRDRKVALAAVVAVLLFGVLNGLLAAIAFSL
                     VWLLKSLASPHLSVPGRVGEHDYVSIERFPQARTVPGM"
BASE COUNT          204 a          579 c          560 g          305 t
ORIGIN      
        1 acccaacaat gacctgctcg agaagctcaa gtccaatctc atggaggtgc gcgcacgcgg
       61 cggggagctc atcgtgttcg cggaccccga ggccggcatc catccgagcg acggcgtaac
      121 cgtcatcgag atgccgaagc acgtcactta cgtacaggcg ccggtggtct acaccattcc
      181 gctgcagctg cttgcctacc atgcggcgat cctgcacggt accgacgtcg atcaaccgcg
      241 caacctcgcc aagagcgtca ccgtcgagta gccggcgagt cacgcgactc gcgcgttaag
      301 tctcgcggcg acgatcgagg ctcccgcgga tcgatgccgc gcggtacgat gcggcagatg
      361 agcgcctccc gcgccgcatc agccgcggat ctgctggcag gactttccat cgccgggttg
      421 ctgctgcccg aggcagtggc ctactcgcac ctcgcgggtc tcccgcagca ggcgggggtg
      481 atcgcgcttt tcgcgggact catctgctac ggcttgatcg gcagcagccg cttcgccatc
      541 gtctcggcga cttcctcctc ggcggcggtg ctcgcctcgg cgatgctcgc cctcggcgcc
      601 accggcgccg cgcagcgtgc cgcgctcgcc ttgcttctcg tggtgggggc gggtatcgcg
      661 tttgcgctgt gcgcagtctt gaagctcggc gccatgtcga acctgatcgc gcgcccggtg
      721 ctgcgcggtt atgccttcgg gctcgcgctc gtgatcgccg cgaagcagtg gccgacgatc
      781 gccgggatgc acacgacgag cgggggcttc ttcccgctga tcgcggagct cgtgcgcggc
      841 ctgcccgcgt ggcgcccgcc ctcgctctat tgcggtatcg ccgcgctcct ggggctgttc
      901 gcgctggagc gtgtacggcg cgtgccgggc gcgctggtgg tgatctgcgc cgggatcctc
      961 gccgccccct ggctcgcgag tcatggggtg gcgctcaccg ggccgatcga cctgactcca
     1021 tctctgccgc agtttgccct cccggagggc acccattggc cgtcgctcgc ggggttctcg
     1081 ctcgccctca tgttcatcct ctacgccgag tcctacggct cgatccgcac ctatgcgctg
     1141 aagcacgacg acgcggtgca gccgaaccgc gatctcctcg cgctcggggt ggccaatgcg
     1201 gtcgcgggat tcctgcaggg gacgcccgtg ggcgcgggtt actcggcgac ctccgcgaac
     1261 gaggcagcgg gcgccgagtc gcgctccgca ggcctcatcg cggctgcggc ggcgctgctg
     1321 ctggtggcgc tgttcctgca ctggatcgag cgcatccccg agccggtgct ggcggcgatc
     1381 gtgatccacg cggtctcaaa gtcgctgcgt ctcgacgtgt tcgctgacta cttccgctgg
     1441 caacgcgacc ggaaggtggc gctcgccgcg gtggtggcgg tgctcttatt cggcgtgctg
     1501 aacgggctgc tcgcggcgat cgccttcagc ctcgtctggc tgctcaagtc gctcgcgagt
     1561 ccgcacctgt cggtgcccgg gcgtgtgggc gagcacgact acgtgagcat cgagcgcttt
     1621 cctcaggccc gtaccgtgcc cgggatgt
//
LOCUS       DATLKL010000887         3165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_78775, whole genome shotgun sequence.
ACCESSION   DATLKL010000887 DATLKL010000000
VERSION     DATLKL010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78775"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /locus_tag="VM713_12040"
     CDS             complement(<1..1760)
                     /locus_tag="VM713_12040"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HVH85033.1"
                     /translation="MSFFTNLRADRLIHEIRTTSDPASPATQKAVASLKQVGPGAIES
                     VFAALPDADKAATMALVEVLAALVTQKTFPQFMQWLIEGSALVISGISWALTSNRNYP
                     AHLLLDALNTQGISKSALLEVITSQKARFSVRELLTAAYAQEPNEKAALFRIIGEIAD
                     QSALPELIGRVQGKDPIARVHIINTLGRFNTPEVQNALQGLLKDPNKLIRSATLSAMQ
                     RMDGPIDVERVCALLRDPELDVLNKAVDVVIKANHPETIRYLIEVLKDENENSRRAAV
                     EVLNEVGDAKSVKYLLEALKDSDWWVRSRAADALGKIGGPKVIDAVLQLVRDKDEDIR
                     RAAIEILNQTKDERAVDSLIQATRDNDWWVSERAVDALAEIGSKRALPRLMEMLRTGS
                     HIKALPIVVRALGRLGDSKLVGELLPLVARPEREIRIEAMQALSKVADESRAEQVRSQ
                     LQAQAASQDQTISRMAAAAVSALDNRLAGIATLAPAGASATPPGMPGTAAPSGVRPPP
                     PAPHEPTRTVVIDDQTRRQAEAAAAAAARLDIQTLKPGDIIEGRYKYIDRIGRGAFGT
                     VLLMEDTVVDERLILKFLNP"
     gene            1996..3074
                     /gene="apbC"
                     /locus_tag="VM713_12045"
                     /pseudo
     CDS             1996..3074
                     /gene="apbC"
                     /locus_tag="VM713_12045"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
BASE COUNT          456 a         1052 c         1129 g          528 t
ORIGIN      
        1 ttggggttga ggaacttgag gatgaggcgc tcatcgacca ccgtgtcctc catcagcagc
       61 acggtgccga acgcgccgcg tccgatgcga tcgatgtact tgtaacggcc ctcgatgatg
      121 tctccgggct tgagcgtctg aatatcgagc cgtgcggcag ctgcggccgc ggcctcggcc
      181 tgacgccgtg tctggtcgtc gatcaccacc gtgcgggtgg gctcgtgggg tgccggcggg
      241 ggcgggcgta cgccgctcgg cgccgccgtg cccggcatgc cgggcggtgt cgcgctggcc
      301 ccggcgggcg ccagcgtggc gatgccggcc aggcggttgt cgagcgcgct gacggccgcg
      361 gcggccatgc gcgagatggt ctgatcctgc gaggccgcct gcgcctgcag ctgcgagcgc
      421 acctgctcgg cgcggctctc gtcggcgacc ttggagagcg cctgcatcgc ctcgatgcgg
      481 atctcgcgct cggggcgtgc aacgagcggc agcagctccc cgaccagctt gctgtcgccg
      541 agcctgccga gcgcgcgcac cacgatcggc agggccttga tgtggctgcc ggtacggagc
      601 atttccatga ggcgcggcag ggcgcgcttg ctgccgatct cggcgagcgc gtccacggcg
      661 cgttcgctca cccaccagtc gttgtcgcgc gtcgcctgga tgagcgaatc gaccgcgcgc
      721 tcgtccttgg tctggttgag gatctcgatg gcggcgcggc ggatgtcctc gtccttgtcg
      781 cgcaccagct gcagcaccgc gtcgatcacc ttcggtccgc cgatcttgcc gagtgcgtcg
      841 gcggcgcggc tgcgcaccca ccagtcgctg tccttgagcg cctccagcag gtacttgacc
      901 gacttggcgt cgccgacctc gttcagcact tcgaccgcgg cgcgccgtga attctcgttc
      961 tcgtccttga gcacttcgat gaggtagcgg atcgtctccg ggtggttggc cttgatgacg
     1021 acgtcgacgg ccttgttgag cacgtcgagc tccgggtcgc gcagcagcgc gcacacccgc
     1081 tcgacgtcga tcgggccgtc catgcgctgc atcgccgaga gcgtcgcgct gcggatcagc
     1141 ttattgggat ccttgagcag gccctgcagc gcgttctgca cctcgggggt gttgaagcgg
     1201 cccagggtat tgatgatgtg cacgcgcgcg atcgggtcct tgccctgcac ccggccgatg
     1261 agctcgggca gcgcgctctg gtcggcgatc tcgccgatga tgcggaacag cgccgccttc
     1321 tcgttgggct cctgcgcgta ggccgcggtg agcaactccc gcaccgaaaa acgcgccttc
     1381 tgcgaggtaa tgacctcgag cagggccgac ttcgagatgc cctgcgtgtt gagtgcatcg
     1441 agcagcaggt gcgcggggta gttgcggttg ctggtgagcg cccaggagat gcccgagatg
     1501 acgagcgcac tgccctcgat cagccactgc atgaactggg ggaaggtctt ctgggtgacg
     1561 agtgccgcga gcacctcgac cagggccatg gtcgcggcct tgtcggcatc gggcagcgcc
     1621 gcgaaaaccg attcaatggc gccggggccg acctgcttca gtgaagccac cgccttctgc
     1681 gtcgcggggc ttgccgggtc ggacgtggtg cggatctcgt ggatcagccg atcggcgcgc
     1741 aggttcgtga aaaagctcat gcgggcgcgc ctggcagccg ccgaggcggc gccgtgcggc
     1801 gccccgtgca cgggcaagag gatggcgtta cagccaggca gccgcgagtg ggtgggttgt
     1861 ggggcgcggg tgtagtcaag ggcgcttgag cgatgggctc gcggcaatta tgccacagcc
     1921 ctgcaaccgc ccccgggttg ggtacaatcc gtcactggcc ccgccggact cttcaacatg
     1981 acccctcagt cccccatgaa cgccgtgcgc gccgcgctcg acgcctatct cgatccgtat
     2041 ctcggcgaga cactcggctc cgcgcaggca gtgcgcgggg tgagcgcagc tcacggaggt
     2101 tacaccgcac agatcgtcct gggttatccc gtcggcggct atcgggagga gctcacggcg
     2161 gctctcacgg cggcgctcgg cgcggcggga atccacacgc cgctcgccct cgagctcgag
     2221 gcggcgatcg gcgctcatgc ggtgcagcgc ggcttgaagc cgctcggtga gatcaaaaac
     2281 atcgtggccg tcgcctccgg caagggtggc gtcggcaagt cgaccgtggc ggtgaatctg
     2341 gcgctcgcct gggccgccca gggggcgcgc gtcgggattc tggacgccga tatctacggt
     2401 ccgagccagc cgctgatgct ggggctcgcg gccgagcggc cggtgtcggc cgacggcaag
     2461 cacctgaagc cgctcgacgc ccacggcgtg agcgccatgt cgatcgggtt tctggtcgat
     2521 gccgagcagc cgatgatctg gcgcggcccg atggtcacgc aagcgctcac ccagctgctc
     2581 agcgagacgc agtggggagc gctcgactat ctggtggtgg acatgccgcc cgggaccggc
     2641 gacatccagc tgacgctcgc ccagcgcgtg ccggtcgcgg gcgccgtgat cgtgacgacg
     2701 ccccaggaca tcgccctcgc cgatgcgcgc aagggctcaa gatgttcgag aaggtcgccg
     2761 ttcccgtgct cggcgtggtc gagaacatga gcgtgcacat ctgcccgaaa tgcggacaca
     2821 ctgaccacat attcggcgca ggtggcgggg cgcgcatggc cgaacagtac ggggtcaagc
     2881 tgctcggcga gctgccgctc gatgcccgca tccgcgagga ggccgacggc gggcgcccga
     2941 cggtggtcgc cgccccggac tcgccgcggg cgcgctgcta cttcgagatg gcgcgccgca
     3001 ccgccggcgc gctcgcgctg cgcgcgcgtg accgcagcag cctatttccg aagatcgtca
     3061 tcgaagaaac ctgactgcac ggtcaatgag catcaagtcc gataactgga tccgccgcat
     3121 ggcacgcgag cagcgcatga tcgagccgtt cgcgcccgag caggt
//
LOCUS       DATLKL010000888         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_67308, whole genome shotgun sequence.
ACCESSION   DATLKL010000888 DATLKL010000000
VERSION     DATLKL010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67308"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VM713_12050"
     CDS             <1..912
                     /locus_tag="VM713_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HVH85034.1"
                     /translation="YILPTRTGMAFGVLWILMLLAGLNYGNSLALFFTFLVAGVALVA
                     MNDCHRNLLGVTLVSVRAPAVFARRVGALGLTLANPAARARPGVQAALDAGPLTGVDV
                     PPHGHAGLELPLAPARRGVVRIDRLRLTSSHPFGLFRTWTWVHLPIEVLVYPHPAGSL
                     PRPITSGSNPGVSSRGASGLDEWMGLRHFRDGDSPRQVDWKAYARGAPLLVKEYSPAG
                     SDLCLFDFAQLPIPDVEARLSQLARWVVDAEAGGERYGLTLPGVELAPNRGPEHRHRC
                     LAALALYGADVPAASGVQLTAAAALHA"
     gene            905..>2336
                     /locus_tag="VM713_12055"
     CDS             905..>2336
                     /locus_tag="VM713_12055"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HVH85035.1"
                     /translation="MPERPDNRALLWAAAAFAGGLALHIDRLPVWVSAAALVLVVWRL
                     AAARARVPLPGVFVRTLLAVAVVIAVLVRFHTLNGLAAGTALLVLMGALKLLETYRRR
                     DRLVLLGAGLFLLLAACLDRQGLGRAPLYLAEVWLACAALAVIATEGFPGRAALALAG
                     RTLLLALPLAVLLFLFFPRLAGSFWAVPRGEEAVTGLSDTMSPGGIGKLSTSYEVAFR
                     ARFEGGLPPAEQRYWRGPVLHYFDGFTWSRGSGEGRAREAPQPAGRAYRYRVALEPSR
                     HRWWFALDTPAQSPDPNVQLTRDFELLAVRPVSEPTSFEALSYTAVRPDATLERWQRI
                     ENTALPRGRNPRSLELANQLRAGAGSDAAFAAAALDFLRRGGFVYSLEPELLGPEQVD
                     DFLFRTRTGFCGHYASAFVVLMRAAGVPARVVTGYLGGEWNPSGGYLIVHQSDAHAWA
                     EVWLEGRGWTRIDPTAVVEPERLRRGA"
BASE COUNT          251 a          853 c          835 g          397 t
ORIGIN      
        1 tacatcctgc cgactcgcac gggcatggcc ttcggcgtgt tatggatcct gatgctgctc
       61 gccggactca attacggcaa cagtctcgcg ctcttcttca cgttcctggt ggccggcgtc
      121 gccctggtgg cgatgaatga ctgccatcgc aacctcctcg gcgtcacgct cgtgtcggtg
      181 agggccccgg cggtgttcgc ccgccgcgtg ggcgccctcg ggctcacgct cgcgaacccc
      241 gccgcccgtg cgcgccccgg cgtgcaggcg gcgctcgatg cagggccgct gaccggggtc
      301 gatgtcccac cgcacggaca cgcgggtctc gagctgccgc tcgcgccggc gcgccgcggc
      361 gtagtgcgca tcgatcggct gcgcctgacg agctcgcatc ccttcgggct gtttcgcacc
      421 tggacctggg tgcacctccc catcgaggtg ctggtctatc cacatcctgc cggctcgctc
      481 ccgcgcccca tcacgagcgg ctctaatccg ggcgtgagct cgcgcggcgc ttccggtctc
      541 gatgaatgga tggggctgag gcactttcgt gacggcgact cgccgcgcca ggtcgactgg
      601 aaagcgtacg cgcgtggggc gccgttgctg gtcaaggaat acagcccggc ggggtccgac
      661 ctgtgcctgt tcgacttcgc gcagctgccc attcccgacg tggaggcacg cctgtcgcag
      721 cttgcccgct gggtggtcga cgccgaggcc ggcggcgagc gctacggact tacgctgccg
      781 ggcgtcgagc tcgcgccgaa ccggggacct gagcatcggc accgttgcct ggcggcgctc
      841 gcgttgtacg gcgccgacgt gcctgccgcc tccggcgtgc agctgaccgc cgcggccgcg
      901 ctccatgcct gagcgcccgg acaatcgggc gctcctctgg gcggcggcgg ccttcgcggg
      961 cggactcgca ctgcacatcg accggttgcc cgtgtgggtc tcggcggcgg ccctggtcct
     1021 cgtggtgtgg cggctcgcag cggcgcgcgc ccgcgtgccg ctgcccggcg tgttcgtccg
     1081 cacgctgctc gccgtggcgg tcgtgatcgc tgtgctcgtc cgatttcaca cgctgaacgg
     1141 cctcgcggcg ggaacggcgc tgctggtgct gatgggggcg ctgaagctgc tcgagaccta
     1201 ccgacggcgc gatcggctgg tgctgctggg cgccggactg ttcctgctgc tggccgcgtg
     1261 cctcgaccgg cagggcctcg ggcgcgcacc tctgtatctt gccgaggtgt ggctcgcctg
     1321 cgcggcgctc gcagtgatcg ccaccgaggg cttcccgggg cgtgcggcgc tcgcgctcgc
     1381 gggacgcacg ctgctgctag ctttaccgct cgcggtactg ctgttcctgt tcttcccgcg
     1441 cctcgccggc agcttctggg ccgtgccgcg cggcgaggag gcggtcaccg ggctctcaga
     1501 caccatgagt ccgggcggca tcggcaagct ctcgacttcc tacgaggtcg ccttccgcgc
     1561 ccgcttcgag ggtgggctgc cgccggccga gcagcgctac tggcgcggcc cggtgctgca
     1621 ctacttcgac ggcttcacct ggtcgcgtgg ctccggggag gggcgcgctc gggaggcgcc
     1681 gcagcccgcc gggcgcgcct accgctaccg agtcgcgctc gagccgagcc gccaccgctg
     1741 gtggtttgcg ctcgacacgc ccgcgcagtc gcccgatccg aacgtccagc tcacccgcga
     1801 cttcgagctg ctcgccgtgc gccccgtctc ggagccgacc agcttcgagg cgctgtccta
     1861 tacggccgtc cgaccggacg cgacgctcga gcgctggcag cgcattgaga acaccgcgct
     1921 gccgcgcggt cggaatccgc gctcgctcga gctggcgaac cagctgcgcg cgggcgccgg
     1981 gagcgatgcg gccttcgcgg ccgccgcgct cgacttcctg cgccgcggag ggttcgtgta
     2041 ttcgctcgag cccgagctgc tcgggcccga gcaggtggac gatttcctgt tccgcacgcg
     2101 caccggcttc tgcggccact atgcctccgc cttcgtagtg ctgatgcgcg ccgcgggcgt
     2161 gcccgcgcga gtcgtgaccg gctacctcgg cggagaatgg aatcccagcg gcggctacct
     2221 gatcgtgcac cagtccgacg cgcacgcctg ggcggaggtg tggctggaag gccgcggctg
     2281 gacccggatt gacccgacgg cggtggtcga gcctgagcgc ctccggcgcg gggcct
//
LOCUS       DATLKL010000889         2324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_112435, whole genome shotgun sequence.
ACCESSION   DATLKL010000889 DATLKL010000000
VERSION     DATLKL010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112435"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VM713_12060"
     CDS             <1..336
                     /locus_tag="VM713_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH85036.1"
                     /translation="GDRGDVTEILGNLLDNACKWCRGRVRLAARNDESAPVRQRLVVT
                     IEDDGPGIAPEDRPRVLQRGVRADENVPGHGLGLAMVRDTVDLYGGRLEVDGSPLGGA
                     RFSLHLPGR"
     gene            complement(389..1927)
                     /locus_tag="VM713_12065"
     CDS             complement(389..1927)
                     /locus_tag="VM713_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVH85037.1"
                     /translation="MSAIDTNAVLARLKLSAVNPGAWSGAHGWSTGTGSALLNVRNPA
                     SGAVIAQVRPATEADYENVITSAQQAASAWREVPAPKRGEAVRLLGEELRRHKSDLGT
                     LVSLENGKILAEGLGEVQEMIDIADFAVGQSRMLYGLSMHSERPRHRMSEQWHPLGIV
                     GIISAFNFPVAVWSWNALLGAICGNACVWKPSHKTPLSALAVQQICNAVMKSAGLPPV
                     FQIFIDGGTGLATRFVEDRRVALISFTGSTEVGRRVGERVAARLGKSLLELGGNNAVI
                     VDETANLDLAVPSIVFGAVGTAGQRCTSTRRVFAHKAIAAELEKRLIHAYKQVRIGDP
                     LEPGTLMGPLIDGKAVESYQAAVSAAQAAGGALLYGGKVLKGAGHFVEPAIVRASNEW
                     PIVQTETFAPVLYLIPVESLAEAFAAQNAAAHGLSSAIFTDRLQSAEAFLSASGSDCG
                     IANVNLGTSGAEIGGAFGGEKDTGGGRESGSDAWKTYMRRQTNTVNWSRELPLAQGIE
                     FKVE"
     gene            complement(1924..>2324)
                     /locus_tag="VM713_12070"
     CDS             complement(1924..>2324)
                     /locus_tag="VM713_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85038.1"
                     /translation="HDRVTLKRILENAVPQTLQDVVIVYAAVTGTQDGQLREENYVNK
                     IYPQVIAGRLWSAIQVATAAGITAVVDLVLAHPARYHGFVAQEQFRLTDIVANRFGRY
                     YAPGGTKHVSGEVVVAGKTGHQRHRKVAVR"
BASE COUNT          357 a          783 c          816 g          368 t
ORIGIN      
        1 ggagaccgcg gggatgtcac cgagatactc ggcaatctgc tcgacaacgc ctgcaaatgg
       61 tgccgcggcc gtgtgcgcct cgcagcgcgc aacgacgaga gcgcgcccgt gcggcagcgg
      121 ctcgtcgtaa cgatcgaaga cgacggcccg ggaattgccc ccgaggaccg cccgcgggtg
      181 ctgcagcgcg gggtgcgcgc agatgagaac gtcccgggac acgggctcgg gctcgccatg
      241 gtgcgcgaca ccgtggatct ctacggcgga cggctcgagg tcgacgggtc gccgctcgga
      301 ggcgcgcgtt tctcgctgca cctgccgggg cgatgaaggt cgacgtgctc agctgtgcga
      361 accgaaacag gtgtgcggga gcggcgggct actcgacctt gaactcgatc ccctgcgcga
      421 gcggcagctc ccggctccag ttgacggtgt tggtctgtcg ccgcatgtag gtcttccagg
      481 cgtccgatcc tgactcgcgg ccgccgccgg tatccttctc gccgccaaag gccccgccga
      541 tctcggcgcc cgaggtgccg aggttcacgt tggcgatacc gcagtcgctc cccgaggccg
      601 agagaaacgc ctccgcgctc tgcagccgat cggtgaagat cgccgacgac aggccgtgag
      661 cggcggcgtt ctgcgccgcg aaggcctcgg cgagtgactc gaccgggatg aggtacagca
      721 ccggagcgaa ggtctcggtc tgcacgatcg gccactcgtt gctggcccgc acgatggcgg
      781 gctcgacgaa gtggcccgcc cccttgagga ccttgccgcc gtacagcagc gctccgcccg
      841 ccgcctgtgc ggcgctgacc gcggcctggt aactctcgac cgccttgccg tcgatcaacg
      901 gtcccatgag ggtccccggc tcgagcgggt caccgatgcg cacctgtttg taggcgtgaa
      961 tgaggcgctt ctcgagctcg gccgcgattg ccttgtgggc gaacacgcgg cgcgtggagg
     1021 tgcagcgctg gccggcggtg ccgacggcgc cgaagacgat cgagggcacg gcgaggtcga
     1081 gattcgccgt ctcgtcgacg atcacggcgt tgttgccgcc caactcgagc aggctcttgc
     1141 cgaggcgtgc ggcgacccgc tcgcccacac ggcgccccac ctcggtggaa ccggtgaagg
     1201 agatgagcgc gacgcggcga tcctcgacga agcgcgtcgc gagcccggtg ccgccgtcga
     1261 tgaaaatctg gaagaccggc ggtaggcccg cggacttcat caccgcattg cagatctgct
     1321 ggacggccag cgcgctcagc ggagtcttgt gcgagggctt ccacacgcag gcgttgccgc
     1381 agatggcgcc gagcagcgca ttccaggacc acaccgccac ggggaagttg aacgccgaga
     1441 tgatgccgac gatgccgagc ggatgccatt gctcgctcat gcgatgccgc gggcgctcgg
     1501 agtgcatcga caggccgtag agcatgcggg actggccgac ggcgaagtcg gcgatgtcga
     1561 tcatctcctg cacctcgccc aggccctcgg cgaggatctt gccgttctcg agcgagacca
     1621 gcgtgccgag atcggacttg tgccgccgca gttcttcccc gagaagccgc accgcctccc
     1681 cgcgtttcgg cgccgggacc tcgcgccagg cgctcgcggc ctgctgagcg ctcgtgatga
     1741 cattctcgta gtctgcttcc gtcgccggac gcacctgcgc gataacggcg ccgctcgccg
     1801 gattccttac gttgaggagc gcactcccgg tgccggtgga ccagccgtgc gcgccggacc
     1861 aggcgccggg gttgacggcc gagagcttga gccgggcgag cacggcgttg gtatcgatgg
     1921 cgctcatcgc acggccacct tgcgatggcg ctgatggccg gtcttgccgg ccacgaccac
     1981 ctcacccgag acgtgcttgg tgcccccggg cgcgtaatag cggccgaagc gattggctac
     2041 gatgtcggtg aggcggaact gctcctgggc gacgaagccg tggtagcggg cggggtgcgc
     2101 cagcaccagg tcgaccacgg cggtaatgcc cgcggcggtc gcaacctgga tcgccgacca
     2161 cagccgcccg gcgatcacct gcggatagat cttgttgacg tagttctcct cgcgtagctg
     2221 cccgtcctgc gtgccggtta ccgccgcata gacgatgacc acgtcctgca gggtctgcgg
     2281 cacggcgttc tcgagaatgc gcttgagcgt gacgcgatcg tgat
//
LOCUS       DATLKL010000890         1967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_224793, whole genome shotgun sequence.
ACCESSION   DATLKL010000890 DATLKL010000000
VERSION     DATLKL010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224793"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VM713_12075"
     CDS             <1..519
                     /locus_tag="VM713_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVH85039.1"
                     /translation="DFHDVRVPVSNLLLGEGRGFEIAQGRLGPGRIHHAMRAVGVAER
                     ALEKMCRRVRARTAFGRPIAEQSVTLERIAESRIRIDQARLLVYKAAYLMDTVGNKAA
                     RAEIAMIKIAAADTACQVTDWAIQAHGAAGLCEDFELASAYAHARAIRLADGPAEVHR
                     NQIGKLELEKHS"
     gene            548..1591
                     /locus_tag="VM713_12080"
     CDS             548..1591
                     /locus_tag="VM713_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HVH85040.1"
                     /translation="MPVPDRQRFDEAALARYLSAHVAGFLGPIKVEMFRGGQSNPTFL
                     LTAGDGAHYVLRRKPAGQLLPSAHAVDREFRITRALFGHGVPVAEPICLCEDASVIGT
                     MFYVMRYVRGRNFWDPRLPGLLREERAAIYDEMNRVLVALHSADFPRLGLADYGKPGN
                     YFARQIARWSKQYRASETDRIDAMEKLLEWLPLNIPPGDETTLVHGDYRIDNLIFAPD
                     RPRMLAVVDWELSTLGHPLADFSYHVMLWRVGAGEIQRLKGVDLGSLGIPTEAEYVAA
                     YCTRTGRALIEPRVWEFCMAYNMFRIACIRQGIMKRVLEGTASSRHAREAGERARETA
                     ELAWRQVEEHLAG"
     gene            1899..>1967
                     /gene="rpsF"
                     /locus_tag="VM713_12085"
     CDS             1899..>1967
                     /gene="rpsF"
                     /locus_tag="VM713_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848538.1"
                     /note="binds cooperatively with S18 to the S15-16S
                     complex, allowing platform assembly to continue with S11
                     and S21; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HVH85041.1"
                     /translation="MRHYEIVFLVHPDQSEQVPAMIE"
BASE COUNT          318 a          671 c          661 g          317 t
ORIGIN      
        1 gacttccacg acgtgcgcgt gccggtctcg aacctgctgc tcggtgaggg gcgggggttc
       61 gagattgcgc aggggcggct cggtcccggg cgcattcatc acgccatgcg ggccgtcggt
      121 gttgccgagc gcgcgctcga gaagatgtgc cgccgcgtgc gggcgcgcac cgccttcggc
      181 cgtccgatcg ccgagcagtc ggtgacgctc gagcgcatcg cggagtcgcg catccgcatc
      241 gatcaggcac ggctcctcgt ctacaaggcc gcgtacctca tggacacggt cggcaacaag
      301 gcggcgcgcg ccgagatcgc gatgatcaag atcgctgccg ccgataccgc ctgccaggtc
      361 accgactggg cgatccaggc gcacggcgcg gcgggcctct gcgaggactt cgagctcgcg
      421 agcgcctacg cgcacgcacg cgccatccgc ctcgccgacg gacccgccga ggtacaccgc
      481 aaccagatcg gcaagctcga gcttgaaaag cactcctgac atgtcagggc aggaattcgc
      541 aggcaccatg ccggtgccgg accggcagcg tttcgatgag gcggcactgg cacgctacct
      601 gagcgcccac gttgccggct tcctggggcc gatcaaggtc gagatgttcc gcggcggcca
      661 gtcgaacccg acgttcctcc tcaccgcggg cgacggcgcg cactacgtcc tgcgccgcaa
      721 gcccgccgga cagctgctgc cctcggcgca cgcggtcgac cgcgagtttc gcatcacgcg
      781 tgcgctcttc gggcacggcg tcccggtggc cgagccgatc tgcctgtgcg aggacgcgag
      841 cgtgatcggc accatgttct acgtcatgcg ctacgtccgc ggtcgcaact tctgggatcc
      901 gcggctcccg gggctgctac gggaagagcg cgccgcgatc tacgacgaga tgaaccgcgt
      961 gctggtggcg ttgcacagcg ccgatttccc gcgactcggg ctcgccgact acggcaagcc
     1021 cggcaactac ttcgcccggc agatcgcgcg ctggagcaag cagtaccgcg cgtccgagac
     1081 cgatcgtatc gacgcgatgg agaagctgct cgagtggctg ccgctcaaca ttccgcccgg
     1141 cgacgagacc accctcgtac acggcgacta tcgcatcgac aacctgatct tcgcgcccga
     1201 tcggccgcgg atgctcgcgg tggtcgactg ggagctctcg acgctcggac acccgctcgc
     1261 ggacttttcc tatcacgtca tgctctggcg ggtcggcgcg ggtgagatcc agagactcaa
     1321 aggcgtcgat ctcggctctc tcgggatccc caccgaggcg gagtacgtcg ccgcttattg
     1381 cacccgcacc gggcgcgcgc ttatcgagcc gcgcgtttgg gagttctgca tggcgtacaa
     1441 catgttccgc atcgcctgca tccgccaggg gatcatgaag cgggtgctcg agggcaccgc
     1501 atcgagccgc catgcgcgcg aggcgggcga gcgcgcccgc gagacggcgg agctcgcctg
     1561 gcggcaggtc gaggagcatc tcgctggctg agcacttcca cgctcgcgcg cccgcccgcg
     1621 ccgggtgccg ccactcctaa atatagaaag gagccgtgcg ggtgcaggag gggtgagggc
     1681 gacggtaagt ccttgatcag gggcccagag ccaagccttt gatcgcaaag ccccgacggg
     1741 tactttgccg ttgcggcgtg caggccgttt gagtatgctt cgcgcccctt tttatccgaa
     1801 ggggcgtccg cgggcgttgg cagccgaggg cgttactcct tgccgcaccg gcgacagcct
     1861 taaccccggg cggctcaacc cgtaaggaga cttaagtgat gaggcactac gaaatcgtct
     1921 ttctcgtgca tcccgatcag agcgagcagg tgcccgccat gatcgag
//
LOCUS       DATLKL010000891         1531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_135169, whole genome shotgun sequence.
ACCESSION   DATLKL010000891 DATLKL010000000
VERSION     DATLKL010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135169"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1531)
                     /locus_tag="VM713_12090"
     CDS             complement(<1..>1531)
                     /locus_tag="VM713_12090"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HVH85042.1"
                     /translation="RPADAIAPLRDAALLEPSNPIIQHDLGLACLEVGRVPDAIAAFE
                     RAVTSNPRYTDAYFRLGIALEKLGSIGGAIVAYDRATELLPSLTEAWFRAGALVYTLG
                     HRDEAIACFRRAAATGGKTSFGRLGKARALLTEDRNQEAEQVLRHTLALDPRNAMAHD
                     LLGNLLSEFGRFDEARECFQRAIAIAPLLAGSYYDLVRCRPVTSDDVGLLQRMETALA
                     TPGLEAAQRLRLHLAIGKAADDLGDYALAMQHFDAADAVRRGSTLFDSAAFSIEIDRL
                     IARCTPELIARAPELGSCDATPVLIIGMPRSGTTLVEQIVSMHPEVGAGGELHFWNER
                     GAAWHRYRGADETPLIAKAAADYLGVLRAIAPKAARVTDKMPFNFLWAGLVHVAFPRA
                     IIIHCRRAAVDTALSIHQTHFHPGLAFPTGGAELVEYFRCYRRITDHWRRVLPSDRFL
                     EVDYEELTRDPEPIIRRMIAACGLPWHDACLRHERNPRAVKTPSKWQTRQPIYRTSVA
                     RW"
BASE COUNT          271 a          482 c          523 g          255 t
ORIGIN      
        1 gccagcgagc gacggaggtg cgatagatgg gctggcgggt ctgccatttg ctcggggtct
       61 tcaccgctcg cgggttgcgt tcgtggcgca ggcatgcgtc atgccagggc aggccgcagg
      121 cggcgatcat ccgtcggatg atgggttctg gatcgcgggt gagttcctcg taatcgactt
      181 cgaggaagcg atccgaagga agcactcggc gccagtggtc ggtgattcgg cggtagcagc
      241 ggaaatactc gaccagttcg gcgcctcccg tcggaaacgc caggcccgga tggaaatggg
      301 tttgatggat cgacagcgcc gtgtcgactg cagcgcgacg gcaatggatg atgattgcgc
      361 gcgggaatgc cacgtggacc agcccggccc acaagaagtt gaacggcatc ttgtcggtca
      421 ctcgcgccgc cttgggcgcg atcgcacgca gcacaccgag atagtctgcc gccgccttgg
      481 cgatcaacgg cgtctcatcg gccccgcggt agcggtgcca cgccgccccg cgctcgttcc
      541 agaagtgcaa ctcaccgcca gccccgacct cgggatgcat ggagacgatc tgctcgacga
      601 gagttgtgcc ggaccgcggc atgccgatga taagcaccgg cgtcgcatcg cagctgccaa
      661 gttcgggcgc ccgcgcgatc aattcgggtg tgcagcgggc aatcaggcga tcgatctcga
      721 tggagaacgc ggccgagtcg aacagcgtag agccccggcg cacggcatct gccgcgtcga
      781 aatgctgcat ggccaaggcg tagtcgccaa gatcgtccgc cgccttaccg atcgcgaggt
      841 gaagccgcag gcgttgcgcc gcttccagcc ccggcgtggc tagggcggtt tccatccgtt
      901 gaagcagacc gacgtcatcg ctcgtgacag ggcgacaccg gaccagatca tagtagcttc
      961 ccgccagcag cggcgcgatc gcgatcgcgc gttggaagca ttcgcgggcc tcgtcgaaac
     1021 ggccgaactc agagagcaga ttgcccagca gatcgtgcgc catggcgttc cgcggatcga
     1081 gcgccagcgt gtgtcgcagc acctgttcgg cttcctgatt gcgatcctcg gtcagcagcg
     1141 cccgcgcctt gcctagacgg ccgaagctgg tcttacctcc ggtcgccgcc gcccggcgga
     1201 aacaggcgat cgcctcgtcg cgatggccca gtgtgtagac cagggcgccg gcgcggaacc
     1261 aggcctcggt cagggatggt agaagttcag tggcgcggtc atacgcaacg attgccccgc
     1321 cgatgctgcc cagcttctcc aaggcgattc ccaagcggaa atatgcatcg gtgtagcgcg
     1381 ggttgctggt gacggcccgc tcgaatgccg cgatggcatc cggtacgcgg ccaacctcca
     1441 ggcaagcgag gccaaggtcg tgctggatga tcggattgga cggctcgagc agcgctgcat
     1501 cgcgcaacgg cgcgatcgca tcggctggtc g
//
LOCUS       DATLKL010000892         2202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_101359, whole genome shotgun sequence.
ACCESSION   DATLKL010000892 DATLKL010000000
VERSION     DATLKL010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101359"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VM713_12095"
     CDS             complement(<1..238)
                     /locus_tag="VM713_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85043.1"
                     /translation="MQLLTLKEAIAQHVRDGDSVALEGFTHLIPFAAGHEIVRLGRRN
                     LHLIRMTPDLLYDQLIGMGCAARLTFSWGGNPGVG"
     gene            complement(245..1849)
                     /locus_tag="VM713_12100"
     CDS             complement(245..1849)
                     /locus_tag="VM713_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVH85044.1"
                     /translation="MPAHAMTAGAHLMTFKLTYATMFDPPETLHARFDAAMQRVRGAL
                     GAAHQLYVAGTDRPAEQTMEKRNPADRGELIGRFAAATAADAERALRAAHDAWPAWRK
                     TPAARRAQLLRRVGQLIEERVYDIAAALTLEVGKNRMEALGEAQEAADFFATYCDDFE
                     RAAGFTRALPDDPLPSHLSHNRSVLKPYGAWVVITPFNFPIALAAGPVAAALITGNTV
                     VLKGATVTPWAGRLLADCIRDAGLPPGVFNYLSGSSATIGAALVDHPLTAGVTFTGSY
                     EIGRQIALKLQGGAYPRPCIAEMGGKNACIVTARADLQRAAAGILRSAFGMGGQKCSA
                     LSRLYVAREVAEPLNEALLGQLATLAIGDPARRENWLGPVATQSGYDDYARFAALLDS
                     GGARILAGGEQLTGGACSRGFFVRPTLAEAPLGHPLWEKEMFLPILMLHRVASNEEAM
                     ALANASPLGLTAGFYGAADEIGWFQENIEAGVTYANRPQGATTGAWPGYQAFGGWKGS
                     GTTGKAIASFYYLPQYLREQSQTVVE"
     gene            1957..>2202
                     /locus_tag="VM713_12105"
     CDS             1957..>2202
                     /locus_tag="VM713_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85045.1"
                     /translation="MVTSLPSASPGAPPALDLPHSYYYREMYLPQLTSGPSSVAWSPD
                     SKELVYSMAGSLWRQRIDASRAEQLTDGPGYDYQPDWS"
BASE COUNT          336 a          771 c          752 g          343 t
ORIGIN      
        1 atccgacccc gggattgccg ccccaggaga aggtcaaccg cgccgcgcac cccatgccga
       61 tcagctggtc gtacaggaga tccggcgtca tgcggatcag gtgcaggttg cgccgcccga
      121 gccgcacgat ctcgtgtccg gcggcgaacg ggatcaggtg cgtgaagcct tcgagcgcca
      181 cgctgtcgcc gtcgcgcacg tgctgcgcga tcgcctcctt gagcgtcagc agctgcatcg
      241 cagctcactc gaccacggtc tgcgactgct cgcgcaggta ctgcggcagg taataaaagg
      301 aggcgatggc cttgcccgtg gtgcccgagc ccttccagcc cccgaacgct tggtagcccg
      361 gccacgcgcc ggtcgtcgcg ccctgcgggc ggttcgcgta ggtgacgccc gcctcgatgt
      421 tctcctggaa ccagccgatc tcatccgccg cgccgtagaa gcccgccgtg aggccgaggg
      481 gggacgcgtt ggcgagcgcc atcgcttcct cgttcgatgc cacgcggtgc aacatcagta
      541 tgggcaagaa catctccttt tcccacagcg gatggccgag cggcgcttcg gcgagtgtcg
      601 ggcgcacgaa aaagccgcga ctgcaggcgc cgcccgtcag ctgctcgcca ccggcgagga
      661 tccgcgcacc gccgctgtcg aggagcgccg cgaagcgcgc gtagtcgtcg tagcccgact
      721 gtgtcgcgac cggacccagc cagttctcgc gccgcgccgg atcgccgatc gcgagcgtcg
      781 caagctgccc gaggagggcc tcgttgagag gctccgccac ctcgcgggcg acatacagcc
      841 gcgagagcgc cgagcacttc tggccgccca taccgaacgc ggagcgcagg atgccggcgg
      901 cggcgcgctg caaatcggcg cgggcggtga cgatacaggc attcttaccg cccatttccg
      961 cgatgcaggg cctggggtac gcgccgccct ggagcttgag cgcgatctgc cggccgattt
     1021 cataggagcc ggtgaaggtg acgccggccg tgagcggatg atcgacgagc gcggcgccga
     1081 tcgtcgcgct cgagccgctc aggtaattga agactcccgg cggcaatccc gcatcgcgaa
     1141 tgcagtcggc gagcagccgc cctgcccagg gcgtcaccgt cgcgcccttc aggaccacgg
     1201 tgttgcccgt gatgagcgca gccgccaccg ggcccgcggc gagcgcaatc gggaagttga
     1261 acggcgtgat gacgacccag gcgccgtagg gcttgagcac gctgcggtta tgggagaggt
     1321 gactcgggag cggatcgtcg gggagcgcac gcgtgaagcc cgccgcgcgc tcgaaatcgt
     1381 cgcagtaagt cgcgaagaaa tccgccgctt cctgcgcctc gccgagcgcc tccatgcggt
     1441 tcttccccac ctcgagggtc agcgccgcgg cgatgtcgta gacgcgctct tcgatgagct
     1501 gcccaacccg ccgcagcagc tgcgcgcgcc gtgcggccgg ggtctttctc cacgccggcc
     1561 aggcatcgtg cgccgcgcgc agcgcgcgct ccgcatccgc cgcggtcgca gcggcgaacc
     1621 tgccgatgag ctcgccgcgg tcagcggggt tgcgcttttc catcgtctgc tcggccggcc
     1681 ggtcggtacc cgccacgtac agctggtgcg ccgcgccgag cgcgccgcgc acgcgctgca
     1741 tggccgcatc gaaacgcgca tgcagcgtct cgggcggatc gaacatggtg gcgtaggtga
     1801 gcttgaaggt catgaggtgc gctcccgctg tcatggcgtg cgcgggcatt gtgctattat
     1861 tcaagcgcta ttcgcacggc gaatcacatt agacgcgtgt cgtctcttcg tcaacgcacg
     1921 cggcgcgcgc actgcagcgc gcaattcctc gcactgttgg tcacttcgct cccgagcgct
     1981 tcgccgggcg cgccgcccgc gctcgatctg ccgcactcct actactaccg tgagatgtac
     2041 ctgccgcagc tcacgagcgg accgagcagc gtggcgtggt cgccggactc gaaggagctg
     2101 gtgtattcga tggccggctc gctgtggcgg cagcggatcg acgcgagccg cgccgagcag
     2161 ctcaccgacg gtcccggcta cgactatcag cccgactggt cg
//
LOCUS       DATLKL010000893         1856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_135182, whole genome shotgun sequence.
ACCESSION   DATLKL010000893 DATLKL010000000
VERSION     DATLKL010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135182"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VM713_12110"
     CDS             complement(<1..732)
                     /locus_tag="VM713_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HVH85046.1"
                     /translation="MNDTTPVKPKKSVALSGVLAGNTALCTVGRTGNDLHYRGYDILE
                     IAEKCEFEEIAFLLIHGKLPTSAELSGYKARLKALRGLPAAVRTVLEALPAAAHPMDV
                     LRSGVSVFGCVLPEKDDHGEAGAREIADRLIASLGSMLTYWYHFSHSGERIHTETQDD
                     SIGAHFLHLLHRRPPPAEWVRAMHTSLILYAEHEFNASTFAARVIAGTGSDLYSCIDG
                     AIGALRGPKHGGANETALVIQQRYES"
     gene            complement(729..1613)
                     /gene="prpB"
                     /locus_tag="VM713_12115"
     CDS             complement(729..1613)
                     /gene="prpB"
                     /locus_tag="VM713_12115"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HVH85047.1"
                     /translation="MPDRAPTAGERLRAAVEAERPLQVVGTINAYSALLAERAGFRAI
                     YLSGAGVANASFGLPDLGVTTLNDVCEDVRRITGKCQLPLLVDADTGFGSAFNIARTC
                     RDLIRAGAGGMHLEDQVSAKRCGHRPGKALVPAEEMVDRLKAAVDARTDADFIVMART
                     DALAVEGLAAALERAAAYVAAGADMIFAEALGTLDEYGQFAARSRVPVLANITEFGQT
                     PLFTLAELASTGVRLVLYPLSAFRAAAKAHEVVYGAIRREGTQKSVVPYMQTRAELYE
                     VLGYHEYERKLDELFGKP"
     gene            complement(1623..>1856)
                     /locus_tag="VM713_12120"
     CDS             complement(1623..>1856)
                     /locus_tag="VM713_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85048.1"
                     /translation="KTTVMPFILRGVALLGINSSATRREWRLAVWQRIATDLRPRQLA
                     RIVTRTIDFEELPQVFPDYLAGRVTGRTLVRIG"
BASE COUNT          285 a          611 c          682 g          278 t
ORIGIN      
        1 ggactcgtag cgctgctgga tcacgagcgc cgtctcgttc gcgccgccgt gcttaggccc
       61 ccgcagcgcg ccgatcgcgc cgtcgatgca cgagtagaga tccgagcccg tgccggcaat
      121 gacgcgcgct gcgaaagtcg aggcgttgaa ctcgtgctcg gcgtacagga tcagcgaggt
      181 gtgcatcgcg cgcacccact ccgcgggcgg cgggcggcgg tgcagcaggt gcaggaaatg
      241 cgccccgatc gagtcgtcct gggtctcggt gtggatgcgc tcgcccgagt ggctgaagtg
      301 ataccagtag gtgagcatcg agccgagcga ggcgatcagc cggtcggcga tctcgcgcgc
      361 gccggcctcc ccgtgatcgt ccttctcggg gaggacgcaa ccgaacaccg acaccccgct
      421 gcgcagcacg tccatgggat gcgccgcggc gggcagcgcc tcgagcacgg tgcgcaccgc
      481 cgccggcagc ccgcgcagcg ccttgaggcg cgccttgtag ccggagagct cggcgctggt
      541 ggggagcttg ccgtggatca ggagaaaggc gatctcctcg aactcgcatt tctcggcgat
      601 ctcgaggatg tcgtagccgc ggtaatgcag gtcgttacca gtgcggccga cggtgcagag
      661 tgcggtattg ccggccagca cccccgagag cgccacggat ttcttcggct tgaccggagt
      721 cgtgtcgttc atggctttcc gaacagctcg tcgagcttcc tctcgtactc gtggtagccg
      781 agcacctcgt agagctcggc gcgcgtctgc atgtagggca cgacgctctt ctgcgtaccc
      841 tcgcggcgga tggccccgta caccacttcg tgcgccttgg cggcagcgcg gaacgccgac
      901 agcgggtaga gcaccaggcg caccccggtg ctcgcgagct cggcgagcgt gaagagcggc
      961 gtctggccga actccgtgat gttggcgagc acgggaacgc ggctgcgtgc ggcgaactgc
     1021 ccgtactcat cgagcgtgcc caaggcctcg gcgaagatca tgtcggcgcc ggcggcgacg
     1081 taagccgcgg cgcgctcgag cgcggccgcg aggccctcga ccgcgagcgc atcggtgcgc
     1141 gccatgacga tgaagtccgc atcggtgcgc gcatcgaccg cggccttcag acggtcgacc
     1201 atctcctcgg cgggcaccag agccttgccg gggcgatggc cgcagcgctt ggcggacacc
     1261 tggtcctcca ggtgcatgcc gccggccccg gcgcggatca ggtcacggca ggtgcgcgcg
     1321 atgttgaagg cgctgccgaa gccggtgtcg gcatcgacga ggagcggcag ctggcacttg
     1381 cccgtgatgc gccgcacatc ctcgcagacg tcgttgagcg tcgtgacgcc caggtccggc
     1441 agcccgaagg aggcgttggc gaccccggcg cccgagagat agatggcgcg aaagccggcg
     1501 cgctcggcga gcagcgccga gtaggcgttg atcgtgccga cgacctgcag cggacgctca
     1561 gcctcgacgg cggcccgcag gcgctctcca gcggtgggtg ctcgatccgg cataggcgaa
     1621 tgttagccga tgcgcaccag ggtgcgcccg gtgacgcgcc cggcgagata gtcggggaag
     1681 acctgaggca gctcctcgaa gtcgatggtg cgggtgacga tgcgcgcgag ctggcggggc
     1741 ctcaggtccg tcgcgatgcg ctgccacacg gcgaggcgcc actcgcggcg ggtggccgag
     1801 gagttgatgc cgaggagtgc gaccccccgc aggatgaacg gcatgacggt cgtctt
//
LOCUS       DATLKL010000894         1511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_56017, whole genome shotgun sequence.
ACCESSION   DATLKL010000894 DATLKL010000000
VERSION     DATLKL010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56017"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1364
                     /locus_tag="VM713_12125"
     CDS             165..1364
                     /locus_tag="VM713_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVH85049.1"
                     /translation="MNDLISKSDPRTSGYTSARDQLKVGGAPLRRRLIIAVLAVVGLI
                     VLIAGIKIYQIMGYIAQAKHNAAPAQTVTATTASYSDWQPEITAVGSVRAVRGVDITT
                     EVTGLVRSVDFRSGEDAKRGQILVHLNADSDIATRHSLEAAADLAATVYARDKVQFEA
                     QAISQAQLDADAADLKNKRAQAAAQAALVEKKTLRAPFDGRLGITTVNPGQYLNTGDK
                     VVTLQELDPVYADFRLPQEQLAQVHPGLEVRLSLDAFPGESFSGKITAVDPLVDSSSR
                     NFQAEATIPNPARKLLPGMFVRVTVLSGAKQHYLTLPQTSITFNPYGSTVFLVQKDDK
                     GKRTAQQTFVTTGPTRGDQIAVLTGIKEGDEVVTSGQLKLKNGTPLDVSTLAQPADAA
                     NPTPQEH"
BASE COUNT          280 a          507 c          497 g          227 t
ORIGIN      
        1 ggcgtcagct acctgcagct gctgaatgcc gagcgtgtgt tcctgcaggc gcggcagaac
       61 cgggtgcagg cggaagcggc acgctacgcg gataccgccg cgcttttcca ggcgctcggc
      121 ggcggctggt ggaacaggca gagcgacggc aaggagaatc gtccgtgaac gatctgatct
      181 cgaaatcaga cccgaggacc tcgggctata catcggcgcg tgaccagctg aaggtcgggg
      241 gcgccccctt gaggcgacgc ttgatcattg cggtcctggc ggtggtcggg ctgatcgtgc
      301 tgattgcagg catcaagatc taccagatca tgggctacat tgcccaggcg aagcataatg
      361 ccgcgccggc gcagacagtc acagccacga cggcgtcgta ttccgactgg caaccagaga
      421 tcacggcggt cgggagcgtg cgcgcagtgc gcggcgtcga catcacgacc gaggtgaccg
      481 gcctcgtgcg cagcgtcgac ttccgctcgg gcgaggatgc caagcgcggc cagatcctcg
      541 tgcacctgaa cgccgactcc gacatcgcca cacgccactc gctcgaggcc gcggccgatc
      601 tcgccgccac ggtgtacgcg cgcgacaagg tgcagttcga ggcgcaggcg atcagccagg
      661 cgcagctcga tgccgatgcc gccgacctca agaacaagcg ggcgcaggct gccgcgcagg
      721 cggcgctggt cgagaagaag accctgcgcg cgcccttcga cgggcggctc ggcatcacca
      781 ccgtgaaccc gggacagtac ctcaacaccg gcgacaaggt ggtgacgctg caggagctcg
      841 accccgtcta tgccgatttc cggctgccgc aggagcagct ggcgcaggtg catccggggc
      901 tcgaggtgcg cctgagcctc gatgccttcc ccggcgagag cttctccgga aagatcactg
      961 ccgtcgatcc gctggtcgac tcctcgagcc gcaatttcca ggcggaggcc accatcccca
     1021 atcccgcgcg caagctcctg cccggcatgt tcgtgcgcgt cacggtgctc tccggcgcca
     1081 agcagcacta tctgacgctg ccgcagacct cgatcacctt caacccgtac ggctcgacgg
     1141 tatttctcgt gcagaaggac gataagggca agcgcaccgc gcagcagacc ttcgtgacca
     1201 ccggcccaac ccgcggcgat cagatcgcgg tcctcacggg catcaaggag ggtgacgagg
     1261 tggtcaccag cgggcagctc aagctcaaga acggcacgcc gctcgacgtg agcacgctgg
     1321 cgcagccggc ggacgccgcg aatccgaccc cgcaggagca ttgagggagc acggcgatga
     1381 aattcacaga tctcttcatc gagcgcccgg tcctctcgat cgtggtcagc ctgctgatcc
     1441 tcgtgctggg cgcacgcgcc gtgtttcagc tgccggtgag ccagtatccg cagaccgaga
     1501 acgcggtggt c
//
LOCUS       DATLKL010000895         2118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_101370, whole genome shotgun sequence.
ACCESSION   DATLKL010000895 DATLKL010000000
VERSION     DATLKL010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101370"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /gene="hrcA"
                     /locus_tag="VM713_12130"
     CDS             complement(<1..513)
                     /gene="hrcA"
                     /locus_tag="VM713_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HVH85050.1"
                     /translation="MAHEARDEALGERAQHLLRILIETYIRDGQPVGSRALSRESGLQ
                     LSSATVRNAMADLEELGFVASPHTSAGRVPTDKGYRFFVDTLLQLRPLDEAATAEIRR
                     QFEASRDSSTDLIAAVSQLLSSATRLAGVVTLPRARSASITQIEFVALSENRVLVVLV
                     LNDREVQNRII"
     gene            612..1496
                     /locus_tag="VM713_12135"
     CDS             612..1496
                     /locus_tag="VM713_12135"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HVH85051.1"
                     /translation="MAFPVRSCALICRFNDTRVTETVNALLPHLKSRGVQVLVAEASP
                     TCEAAGAMRVPPASIGERADLVIAVGGDGTLLHAARLVARHGVPLLGVNRGRLGFLTD
                     IMPQDLLAAVDAALRGELEKDERPLLAAQLHSAGGGIAQALALNDVVMQKHDTGRTLE
                     FETRIDQRYVNTHHGDGIIVSSATGSTAYALSCGGPIVEPHLDALVIAPICAHTLSDR
                     PIVVSSRSVIEISLLERLDTQANVSCDGMRLGALQAGDRLEVRTAKERVTLLHPPGHD
                     YYTLLRSKLHWGRGALGN"
     gene            1507..>2118
                     /locus_tag="VM713_12140"
     CDS             1507..>2118
                     /locus_tag="VM713_12140"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVH85052.1"
                     /translation="MLTHLQIRDFAIIDAVELELRAGLTVLTGETGAGKSIVVDALQL
                     LAGGRAGAEVVRHGAERAEVSATFDLTQAPRELKQWLEEQSIGAGAELIMRRVVGNDG
                     RSRAYLNGQSVPLQSLRDAGNILIDIHGQHEFQSLTRSAQQRELLDGYGALTGLTSQV
                     GIAHRVWLGLLNRTLELESKARDRDARLDLLRYQVQELEGLHLK"
BASE COUNT          330 a          744 c          731 g          313 t
ORIGIN      
        1 gatgatgcgg ttctgcacct cgcggtcgtt gagtaccagc accaccagca cccggttctc
       61 ggacagcgcc acgaactcga tctgggtgat cgaggccgag cgggcgcgcg gcagggtgac
      121 gactccggcg agccgggtcg cgctcgagag cagctgcgac acggccgcga tcaggtcggt
      181 ggagctgtcg cgactcgcct cgaactggcg gcggatctcg gcggtggccg cctcgtcgag
      241 cgggcgcagc tgcagcagcg tgtcgacgaa gaagcggtaa cccttgtcgg taggcacccg
      301 gccggcggag gtgtggggcg aggcgacgaa gcctagctcc tcgagatccg ccatcgcgtt
      361 gcggaccgtg gcggaggaaa gctgcagccc cgactcgcgc gacagggcgc gcgagccgac
      421 cggctgaccg tcgcggatgt aggtctcgat caggatccgc agcaggtgct gagcccgctc
      481 acctaaggcc tcgtcccgag cttcgtgcgc catatgagtg ttgcgtcgga ccttacatca
      541 tgcacagccc gcgtgctaca aaggccccgc ctcgcgatgc tggcgaggcg tctggttgag
      601 gcttgaagct tatggctttt cccgttcgca gctgcgcgct gatctgcaga ttcaacgata
      661 cgcgcgtgac cgagaccgtg aatgccctgc taccgcacct gaagagccgc ggcgtgcagg
      721 tgctggtcgc ggaagcgtcc cccacctgcg aggccgcggg cgccatgcgc gtcccgccgg
      781 cctcgatcgg ggagcgcgcc gacctcgtca tcgccgtggg cggcgacggc acgctgctgc
      841 acgcggcgcg cctcgtggcc cgccacggcg tgccgctgct cggagtcaac cgcggccggc
      901 tcgggttcct caccgacata atgccgcagg acctgctggc ggcggtcgat gcggcgctgc
      961 gcggcgagct cgagaaggac gagcgaccgc tgctcgccgc acaactgcac tcggccggcg
     1021 ggggcatcgc ccaggcgctg gcgctcaatg acgtcgtcat gcagaagcac gatacggggc
     1081 gcacgctcga attcgagacg cgcatcgacc agcgatatgt caacacccac cacggcgacg
     1141 gcatcatcgt ctccagcgcc accggctcga ctgcctatgc gctctcgtgc ggcggcccga
     1201 tcgtcgagcc gcacctcgac gcgctggtga tcgcgccgat ctgcgcgcac acgctgtccg
     1261 accgtccgat cgtcgtgtcc tcgcgctcgg tcatcgagat aagtctgctc gagcgtctcg
     1321 acacccaggc gaacgtctcc tgcgacggca tgcggctcgg agccctccag gcgggcgacc
     1381 ggctcgaggt gcgcacggcg aaggaacggg tgacgctgct gcacccgccc ggacacgact
     1441 actacacgct gctgcgctcg aagctccact ggggccgcgg cgccctcggg aactgaaggc
     1501 ggcggcttgc tgacgcacct gcagatccgg gacttcgcca tcatcgacgc ggtggagctc
     1561 gagctgcgcg ccgggctcac ggtcctcacc ggcgagaccg gcgccggcaa gtcgatcgtc
     1621 gtcgacgcgc tgcagctgct cgccggcggg cgcgcgggcg ccgaggtggt gcgccatggt
     1681 gcggagcgcg ccgaggtgtc cgccaccttc gatctcacgc aggcgccgcg cgagctcaag
     1741 cagtggctcg aggagcagtc gatcggcgcc ggcgccgagc tcatcatgcg ccgcgtggtc
     1801 gggaacgatg gccgctcgcg cgcttacctc aacggccagt cggtcccgct gcaatcgctg
     1861 cgcgatgccg gcaatatcct catcgatatc catggccagc acgagttcca gtccctgacg
     1921 cgcagcgccc agcagcgcga gttgctcgac ggctatggcg cgctcacggg gctcaccagc
     1981 caggtcggga tcgcacaccg cgtctggctc ggactcctca accgcacgct cgagctcgag
     2041 agcaaggcgc gcgaccgcga cgccaggctc gacctgctgc gctaccaggt gcaggaactc
     2101 gagggcctgc acctcaag
//
LOCUS       DATLKL010000896         1542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_135202, whole genome shotgun sequence.
ACCESSION   DATLKL010000896 DATLKL010000000
VERSION     DATLKL010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135202"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1542
                     /gene="glnE"
                     /locus_tag="VM713_12145"
     CDS             <1..>1542
                     /gene="glnE"
                     /locus_tag="VM713_12145"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HVH85053.1"
                     /translation="LVRIAWRDLAGWAELGETLADLSAFADAAIRTAVSYARHALVAR
                     YGEPRSAGGEVQPLVVIGMGKLGGGELNFSSDVDLVLLFPEHGETDGTRGVANEEFFT
                     RLVQALVRLLEAPTAEGFVLRVDLRLRPFGDSGPVVSSFASFEDYLPRHGRDWERYAY
                     IKARAITAADRYAEIEASAIRPFVYRRYLDYGVFESLREMKALIAREVERRELADDIK
                     LGPGGIREVEFIVQALQLTRGGRDRSLQTPALRTALARLGATRILPADVVAQLDAAYV
                     YLRRLENRLQMLGDAQVHALPVEPAARERIALAMGARDWPALLAELARHRECVRGHFR
                     DVILGGGAQDDAAVRIDLGRFWDSRAETAALAESLAHAGFTDSAEAARLLLELRASSL
                     VRKLDEPGRRRLQALLPVLLADVARSGAQLPVLRRILTIIEATGKRSAYFALLRENAA
                     ARARLVELLGHGEFLAAQIAAQPLLLDELIDARLLSELPTRASLAAELGQRLAELHDE
                     DPERQ"
BASE COUNT          192 a          543 c          569 g          238 t
ORIGIN      
        1 agctggtgcg catcgcgtgg cgcgacctcg ccggctgggc cgaactcggc gagacgctcg
       61 ccgatctcag cgccttcgcc gacgcagcga tccgcacggc ggtgtcttac gcgcgtcacg
      121 cactggtcgc gcgctatggc gagccgcgtt ccgccggcgg tgaggtccag ccgctcgtgg
      181 tgatcggcat gggcaagctc ggcggcggcg agctcaattt ctcctcggac gtcgacctgg
      241 tgctgctgtt ccccgagcac ggcgagaccg acggcacgcg cggcgtggcc aatgaggaat
      301 tcttcacgcg tctcgtgcag gcgctggtgc ggctgctcga ggcgccgacc gcggagggct
      361 tcgtgctgcg ggtggacttg cgcctgcggc ccttcggaga ttcggggccc gtcgtgtcga
      421 gcttcgcctc cttcgaggac tatctgccgc ggcacggccg cgactgggag cgttacgcgt
      481 acatcaaggc gcgcgcgatc accgctgccg accgctatgc cgagatcgag gcgtccgcga
      541 ttcggccgtt cgtctatcgg cgctatctcg attacggagt gttcgagagc ctgcgcgaga
      601 tgaaggcgct catcgcgcgg gaggtcgagc ggcgcgagct cgccgacgac atcaagctcg
      661 gtcccggcgg tattcgtgag gtcgagttca tcgtgcaggc gctgcagctc acgcgcggcg
      721 gccgcgatcg cagcctgcag acgccggcgc tgcgcaccgc gctcgcgcgg ctcggcgcaa
      781 cacggatcct gccggccgac gtggtggcgc agctcgatgc cgcgtacgtg tatctgcgcc
      841 gcctcgagaa ccggctgcag atgctcggcg atgcccaggt gcatgcgctg ccggtggagc
      901 ccgcagcgcg cgagcgcatc gcgctcgcca tgggcgcgcg cgactggccc gcgctccttg
      961 cggagctcgc tcgccatcgc gagtgtgtgc gcggacactt ccgcgacgtc atcctgggcg
     1021 gcggcgcgca ggacgatgcc gcggtccgga tcgacctcgg aaggttctgg gacagccggg
     1081 cggagacggc ggcgctcgcg gagtccctcg cgcacgctgg cttcaccgac agcgccgagg
     1141 cggcgcgcct gctgctcgag ctgcgcgcct cgagcctggt gcgcaagctc gatgagccgg
     1201 ggcgccggcg gctccaggcg ctcctgccgg tgctgctcgc cgatgtggcg aggagcggcg
     1261 cgcagctgcc ggtgctgcgc cggatcctca ccatcatcga agcgaccggc aagcgctcgg
     1321 cctactttgc gctgctgcgc gagaacgccg cggcgcgcgc gcgcctggtc gagctgctcg
     1381 ggcacggcga gttcctcgcg gcgcagatcg ccgcgcagcc gctgctgctc gatgagctga
     1441 tcgacgcgcg gctcctgtcg gagctgccga cccgcgcctc cctcgcggcg gagctcggcc
     1501 agcgcctcgc tgagctgcac gacgaggacc cggagcgcca gg
//
LOCUS       DATLKL010000897         1587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_146399, whole genome shotgun sequence.
ACCESSION   DATLKL010000897 DATLKL010000000
VERSION     DATLKL010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146399"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="VM713_12150"
     CDS             <1..1369
                     /locus_tag="VM713_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVH85054.1"
                     /translation="SYGTTEQSGLSDATSIAAAPGTGATGTFGNVVTTLLPLVPPPPP
                     AGATAIAGLAETQSTDIKKWTQEFRLASAAGQTLEWQLGAFYTRESSALDQTLPLFYI
                     PTLVYVPLPLAFGGPALENASLDSLYREWAGFAQFTYHFSPQWDLALGGRYSENKQSA
                     NEVVTGVLPTLQGTPQQLTAGDSSDNSFTYSIAPRWHVSSDTMVYGRIATGYRPGGPN
                     DLPFNAPASVQRSYEPDKTTNYELGVRTDVLDKTLSVDVAAFLVDWKKIQLFEFVQVA
                     GTPGFGINANGGTARSKGLEWTLGLTPVTGLAFTLTGAYVDAYLTSAASAAGGNDGDP
                     LPYVPKWSTSLDGAYTWRAFSDYNAFAGATWSYTGSRFNDFSATATAAGFVPDPRPEL
                     PSYSTVNLRAGLENGRWAFELYCKNVGDTRGIAYYTNSGTPNYGGTVNYVMPRTLGAT
                     ATLKF"
     gene            1400..>1587
                     /locus_tag="VM713_12155"
     CDS             1400..>1587
                     /locus_tag="VM713_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85055.1"
                     /translation="MIERAAGSARGAAAGESTRLPSPYLLFLGDATEPAFAKTAFGLR
                     DWIPERCVGEWACEEATVS"
BASE COUNT          256 a          558 c          507 g          266 t
ORIGIN      
        1 gagctacggg acgaccgagc agagcgggct ctctgatgcc acttcgatcg ccgccgcacc
       61 cgggaccggc gcgaccggta cgttcggcaa cgtcgtgacg accctcttgc cgctcgtccc
      121 gcctccgccc cctgcggggg ccaccgccat tgccggcctc gccgagaccc agtcgaccga
      181 catcaagaag tggacccagg agtttcgtct cgcctccgcg gccgggcaga cgctcgagtg
      241 gcagctgggg gcgttctaca cgcgtgagtc atcggcgctc gaccagacgc tgccgctctt
      301 ctacatcccg acgctggtgt acgtcccgct gccgctggct tttggcggtc cggcgctcga
      361 gaacgcatca ctggactccc tgtaccgcga gtgggcaggt tttgcgcagt tcacctacca
      421 cttcagcccg cagtgggacc tggccctcgg cggccgctat tccgagaaca agcagtccgc
      481 caacgaggtc gtcaccggcg tgctgcccac gctccagggc acgccgcagc agttgaccgc
      541 cggcgattcg agcgacaatt ccttcaccta ctcgattgcg ccgcgctggc acgtgagcag
      601 cgacaccatg gtgtatgggc gcatcgcgac cggctatcgg ccgggcggcc cgaacgacct
      661 gccattcaat gcgccggcca gcgttcagag atcgtacgag cccgacaaga cgaccaacta
      721 cgagctcggc gtacgcacgg acgtgctcga caagaccctg tccgtcgatg tcgccgcgtt
      781 cctcgtcgac tggaagaaga ttcagctgtt cgaattcgtc caggtggcgg ggacacccgg
      841 gttcggcatc aacgccaacg gcggcaccgc gcgcagcaag ggtctcgagt ggacgctggg
      901 gctcacaccc gtcaccgggt tggccttcac tctcaccggc gcgtacgtcg atgcttacct
      961 cacgtccgcg gcgtccgcag ccggcggcaa cgacggcgat ccactcccgt acgtgccgaa
     1021 atggagcacg tcgctcgacg gggcctacac gtggcgtgcg ttcagtgatt acaacgcgtt
     1081 cgccggggcg acctggagct acaccggctc gcgcttcaac gatttctccg ccaccgcgac
     1141 tgcagccggg ttcgtaccgg atccgcgccc cgagttgccg agctacagca ccgtcaacct
     1201 gcgcgcgggg ctcgagaacg gtcgctgggc gttcgagctg tactgcaaga acgtgggcga
     1261 tacccgcggc atcgcctact acactaactc gggtaccccg aactacggag gcacggtcaa
     1321 ctacgtaatg ccgcgcactc tcggcgcgac ggccacgctg aagttctgag gggcggttcc
     1381 gctgtcagtt ggtgccaccg tgatcgagcg cgccgcgggc tctgctcggg gagctgctgc
     1441 gggcgaatcc acgcggctgc cgtctccgta cctgctgttc ctcggcgatg ccaccgagcc
     1501 ggccttcgcc aagacggcgt ttgggttgcg cgactggatc cccgagcgct gcgtcggcga
     1561 gtgggcatgc gaggaggcca cggtctc
//
LOCUS       DATLKL010000898         1534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_101400, whole genome shotgun sequence.
ACCESSION   DATLKL010000898 DATLKL010000000
VERSION     DATLKL010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101400"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="VM713_12160"
     CDS             <1..1383
                     /locus_tag="VM713_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVH85056.1"
                     /translation="SGIPMANIGILTRTSAAPPGPRGLPLLGVLSELRRDTLEFIRRV
                     GRQYGDLITLDLGVERVWLLNHPDHVRRVLHDNYRNYRKSDYYKRSKPLVGLGLLTSE
                     GELWRRQRMLMQPAFRRERLPHYVATMVACAEGALTRWAAAAARGEALPLGREIMRLT
                     RTILIRALFGAAMSGGEVRIDRALETVLAFHEARVWSLLPLPTALPTPANLRYRAALK
                     TLNDLVRSLILACRARGTPGDDLLSLLVFARDESTGEGMSEQQLRDEVMTIFIAGHET
                     TALATLWTLHLLSQHEVERVRLEAEIDAVLASRPPGVADLGRLAYAAQVVQESMRLYP
                     PLWTISRAAIAEDEIGGYVIPAGTTVMICPYLIHRHPEVWRDPERFDPDRFSTPEVER
                     RHPYAYFPFAAGPRKCIGAQLGLTEAVVLTAMICQRFRLRLTAGETVVPRAMMTLRPE
                     REIMVELKPR"
BASE COUNT          243 a          548 c          501 g          242 t
ORIGIN      
        1 agcggcattc ccatggcaaa catcggcatc ctcacccgca cttccgccgc accacccgga
       61 ccccgcggtc tcccgctcct cggcgtgctc tccgagttgc ggcgcgacac actcgaattc
      121 atccgccgcg tcggccgaca gtacggcgac ctcatcactc tcgatctcgg agtcgagcga
      181 gtctggctgc tcaaccaccc ggaccacgtg cgccgggtgc tgcacgacaa ttaccggaac
      241 taccggaaga gcgactacta caagcgctcg aagccgctgg tgggcctcgg attgctcacc
      301 agcgaaggcg agctttggcg acgccagcgc atgctgatgc agcccgcttt ccggcgcgag
      361 cggctcccgc actatgtggc gacgatggtc gcgtgcgccg aaggcgcgct gacccgatgg
      421 gcggcagcgg ccgcgcgcgg cgaggcgttg ccgctcggcc gcgagatcat gcggctcact
      481 cgaaccattc tgattcgcgc gctgttcggc gccgcgatga gtggcgggga ggtgcgcatc
      541 gatcgtgcgc tcgagaccgt gctggcattc cacgaggcgc gagtctggtc gttgctcccg
      601 ctcccgacgg cgctgcccac cccggcgaac ctgcgctacc gggcggccct gaagaccctg
      661 aatgacctgg tgcgaagcct gattctcgcc tgccgcgcgc gcggtacacc gggcgacgac
      721 ctgctctccc tgctcgtgtt tgcacgggac gagagcacgg gcgaaggcat gagcgagcaa
      781 cagctgcgcg acgaggtgat gacgatattc atcgccggtc acgagacgac ggcgctggcc
      841 acgctgtgga ccctccacct gctctcgcag cacgaggtcg agcgcgtgcg tctcgaggcc
      901 gaaatcgacg ctgtgctcgc gagccgcccg cccggcgtcg cggacctcgg ccggctcgcc
      961 tacgccgcgc aggtggtgca agaatcgatg cggctctatc cgccgctctg gaccatctcg
     1021 cgcgcggcga tcgccgagga cgaaatcggc ggctacgtca ttccggccgg aacgaccgtg
     1081 atgatctgcc cctatctcat ccaccgtcac cctgaggtct ggcgcgaccc ggagcgcttc
     1141 gacccggacc ggttctcgac gccggaggtc gaacggcgcc acccctatgc gtacttcccc
     1201 ttcgctgcgg gcccgcgcaa gtgcatcggc gcccaactcg ggctgaccga ggcggtcgtg
     1261 ctgaccgcca tgatatgcca gcgatttcgc ctgcgcctga cagcgggcga gacggtcgtg
     1321 ccgcgggcca tgatgacgct gcggccggag cgcgagatca tggtggaatt gaagccacga
     1381 tagccccaag ggccgcgccg ccgcgcgcgg gcgcaataat gcggcttatt gcgcggctgc
     1441 tgcccggccg atcgcgggcg gcgccgacgc gatctcgccc accagcttgt tcacgatcgc
     1501 ctggccgaac gagttgaagt catcgatcac cagc
//
LOCUS       DATLKL010000899         1598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_90120, whole genome shotgun sequence.
ACCESSION   DATLKL010000899 DATLKL010000000
VERSION     DATLKL010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90120"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..807
                     /locus_tag="VM713_12165"
     CDS             49..807
                     /locus_tag="VM713_12165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVH85057.1"
                     /translation="MDAASVVQLQRSSDFRARVLEISAQLRAAVVNGHDADITRLLGE
                     LVHFRGSSRAQSDALQLRALQSLVYLLRSAATNDELTGLLNRRGFLQCATRLLDLAVR
                     DRCPHRLIYFRLSPRQDGAAFAPMALRQMGNALRDLFPDYGMREALGRLGGAEFAALT
                     PRVEYLACDSILRHIATTAATLGPAGTRLDLGIARFDPQGPVEIDELLARCMEQALRS
                     RELFRRTASFASGPPTRSDASLTVSGRRFASCKD"
     gene            1127..1417
                     /locus_tag="VM713_12170"
     CDS             1127..1417
                     /locus_tag="VM713_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85058.1"
                     /translation="MMTTDESSARSAPVTPAAPDEAQYLRWVAEKVRSEDFWTAVQED
                     ILENFANLLNRELPDELWRAAYAQRDGKDYMIAFAAVLERLEEYCGRPPRHG"
BASE COUNT          242 a          540 c          544 g          272 t
ORIGIN      
        1 ccttccactg gcgctggtcg atgttcgtgg gcgggaggga ctctaacggt ggatgcggca
       61 tcggtcgtac aactccaacg cagcagcgac ttccgcgccc gcgttctcga gatctcagcc
      121 caactgcgtg ccgcggtcgt caacgggcac gacgcggaca tcactcgtct gctcggagag
      181 ctggtgcact tcaggggatc gagtcgcgca cagagcgacg cgctgcagct gcgggcgctc
      241 cagagtctgg tgtacctgct gcgctcggcc gccacgaatg acgagctgac cggcctcctc
      301 aaccgccgcg gcttcctcca gtgcgccacc cggctgctcg acctggcggt ccgcgaccgc
      361 tgtccgcacc gcctcatcta tttccgtctc agcccgagac aggacggcgc agcgttcgcg
      421 ccgatggcgc tgcggcagat gggcaacgcg ctgcgcgatc tctttccgga ctacgggatg
      481 cgcgaggcgc tcgggcggct cggtggcgcg gagttcgccg ccctgacccc gcgcgtcgag
      541 tatctcgcgt gcgactcgat cttgcggcac atcgcgacaa cggcggcgac cctcggtccc
      601 gccggtactc ggctcgacct cgggattgcg cgcttcgatc cccagggtcc agtggaaatc
      661 gacgagctgc tcgcgcgctg catggagcaa gcgctgcgtt cgcgcgagct gttccgccga
      721 actgcgtcct tcgcatcagg ccccccaacg cggagtgacg catcgctgac ggtctcgggg
      781 cggcggtttg cgtcgtgtaa ggattgacac ggcggcgccg cctctgagac cgctttttct
      841 cgacagcctg tcagcgccat cgcacgcgcg cgcgggaatt cagcggacgg cctgcgtgcc
      901 ggtggctcgt tacgctccgt gacgcgtatg cagcggccgg agggatcatg gccatttcga
      961 agtcccgcga cgatcactgc cgggactagc ggataggtct agccgcacca gggacggtgc
     1021 ggcgaccagg gcgaaaaagt cccgcaaccc ctcccgcctg aggaagaatc atcgctcgcc
     1081 agtcgtatga atcgacatac cggcagaggt ttccggggag tcgaccatga tgacgacgga
     1141 tgagtcgagc gcgcgctccg ccccggtcac cccggcggcg ccggacgagg cgcagtatct
     1201 gcgctgggtg gccgagaagg tccggagcga ggacttctgg acggcggtgc aggaggacat
     1261 cctcgagaac ttcgccaatc tgctgaaccg cgagctgccc gacgaattgt ggcgcgcggc
     1321 ctacgcgcag cgtgacggaa aggactacat gatcgcgttt gcggctgtgc tggagcggct
     1381 cgaggagtat tgcggccgcc cccccaggca cggttagcac cggtcagcca gcgcgtgcgc
     1441 ggcgccgcgc ttccacgcta acatcgcgca tgcattactt tccggtcacc gcgccggtgc
     1501 tgctcgggct ggcggtgctg tttgcgatcg tcgccggctc ggtcgcggcg cggattctcg
     1561 gcttcgcctc ggccagtatg gggctcgcac cggggacc
//
LOCUS       DATLKL010000900         2284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_56154, whole genome shotgun sequence.
ACCESSION   DATLKL010000900 DATLKL010000000
VERSION     DATLKL010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56154"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..2137
                     /locus_tag="VM713_12175"
     CDS             101..2137
                     /locus_tag="VM713_12175"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HVH85059.1"
                     /translation="MDLSQVQRFSIGLDTGGTYTDAVALDSRQQVVASAKALTTHWDL
                     SIGLHAALRAVLAQLPAGVAREHASLVSVSTTLATNAVVENRFSPICTILIGFDERMV
                     ERTGLKRAGGGILVRVQGGHEATGEESAPLDEAAVDAAVSEFGGQVEAFAVAATFSVR
                     NPSHEQRARERIRARCPKPVTCSFELSSQLDAPKRALTAALNARLTPQIHHLLGALDG
                     VLDAEGISAPVMIVRGDGTLIRAAVALEYPVETVLSGPAASVVGAGFLTGLRAFAVAD
                     MGGTTTDVAIVADGAPVVRGEGAVIGGWRTMVEAIDVHTCGLGGDSEVYLDRDGRLVV
                     GPRRVMPLSLLAERSPEVLAQLRKLAAGERLPPFAGRFAYRNSEEDSASPLDRLEQRV
                     WAGLGAAPRPLGDVARTTPGVEAVRRLVDRGLATVAGFTPSDAMHVLGRQQGWNVEAA
                     RLGAAVLALEERNVSARRELAAPEGLSERVYQQVVREAARAVLECALAEDPGIEPAHG
                     RWGPLGRLLEDVVAGRPFSRLIDAGLRLGAPLVAIGAPAGAYFPEVARRLGATLQVPA
                     HAAVCNAVGAAVGVVSETCEILVNQPAFKVFRVHDPAGNRDYGDAAEAIEEAKRISRE
                     LALAAARRAGATEPRLETSVAERRARVGAGEDGDYLAEATVRSTATGRPAARGA"
BASE COUNT          315 a          792 c          852 g          325 t
ORIGIN      
        1 cgcgcacgtc gcctgtcatc cggatggggc aggcgggccg gtgaggcgcc cgggtgcgcc
       61 agctggtcca tggcatgccg tcactggcac actattccgc gtggacctga gccaggtgca
      121 gcgcttctcg atcggactgg acaccggcgg cacatacacc gacgcggtcg ctctcgattc
      181 ccgacagcag gtggtggcga gcgccaaggc gcttaccacc cactgggatc tctccatcgg
      241 gctgcacgcg gcactgcgcg ccgtgctggc gcagctcccc gcaggcgttg cgcgcgagca
      301 cgcctcgctc gtatcggtct cgacgacgct cgcgaccaat gcggtcgtcg aaaaccgctt
      361 cagtcccatc tgcacgatcc tgatcggctt cgacgagcgc atggtcgagc gcacggggct
      421 gaagcgcgcc gggggaggaa tcctcgtgcg ggtgcagggc ggtcacgagg cgacgggcga
      481 agagtccgcg ccgctcgacg aagccgccgt cgatgcggcg gtgagcgagt tcggcgggca
      541 ggtcgaggca ttcgcggtcg cggcgacgtt ctcggtgcgc aatccctcac acgagcagcg
      601 cgcgcgcgag cggatccgcg cacgctgccc gaagccggtc acctgcagct tcgagttgag
      661 ctcgcagctc gacgcgccga agcgggcgct gacggcggcg ctcaatgcgc gcctgacgcc
      721 gcagattcac cacctgctcg gcgcgctcga cggtgtgctc gatgccgagg gcatctccgc
      781 accggtgatg atcgtcaggg gagacggcac cctgatcaga gcggcggtcg ccctcgaata
      841 ccccgtcgag accgtgctgt cgggacccgc cgccagcgtc gtgggagcgg gcttcctcac
      901 gggactccgc gctttcgccg tggccgacat gggtggcaca acgaccgacg tcgcgatcgt
      961 cgccgacggc gcccccgtcg tgcgcggcga aggcgccgtg atcggcggct ggcgcaccat
     1021 ggtcgaggcg atcgacgtgc acacctgcgg cctgggcggc gacagcgagg tgtacctcga
     1081 ccgcgacggg cgcctcgtcg tggggccgcg cagggtgatg cccttgagcc tgctcgccga
     1141 gcgctccccg gaggtcctgg cgcagctgcg caagctggcc gccggcgagc gcctgccgcc
     1201 attcgcgggc cgctttgcct atcgaaactc cgaagaggat tccgcctcgc cgctcgatcg
     1261 gctcgagcaa cgggtatggg cagggctcgg agccgccccg cggcctctcg gcgatgttgc
     1321 gcgcaccact cccggggtcg aggcggtccg ccggctcgtc gaccgcggac tggcgacggt
     1381 ggcgggattc acgccaagcg atgccatgca cgtgctcggc cggcagcagg gctggaacgt
     1441 cgaggccgcg cggctcggcg ccgccgtgct cgcgctcgag gagcgcaatg tcagcgcgcg
     1501 ccgcgagctg gctgcgcccg aggggctcag cgagcgtgtc taccaacagg tagtgcgcga
     1561 agccgcgcgg gccgtgctcg agtgcgctct cgcggaggac ccgggcatcg agccggcgca
     1621 cgggcgctgg ggcccgctcg ggcgtttgct cgaggatgtg gtggcggggc ggccgttctc
     1681 acggctcatc gacgccggcc tgcgactcgg cgcgccgctg gtcgcgatcg gcgccccggc
     1741 cggcgcctac ttccccgagg tggcgcgccg gctcggcgcc acactgcagg tgcccgcgca
     1801 cgccgccgtc tgcaacgcgg tcggggcggc agtcggggtg gtctcagaga cgtgcgagat
     1861 tctggtgaac cagccggcct tcaaggtctt ccgggtgcac gacccggccg gcaaccggga
     1921 ttatggcgat gcggccgagg ccatcgagga ggcgaagcgc atatcacggg agctggcgct
     1981 tgccgccgcg cgccgtgccg gcgcgactga gccgcggctc gagacttccg tggccgagcg
     2041 gcgcgcgcgc gtgggtgccg gggaggacgg cgactatctt gccgaggcga ccgtgcgatc
     2101 cacggcgacc ggccggccgg cggcgcgggg agcgtgagcg gatgcgccgc gcgtcgggtt
     2161 ggtagtaacc tgcaggcaca atgaaaaacg cagtgctccc gatctgcgga ctcgcctggc
     2221 tcagcgcaac gagcttcgca tagtggatcc accgcgatta cgggctctcc gagctcgacg
     2281 ccta
//
LOCUS       DATLKL010000901         1738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_33751, whole genome shotgun sequence.
ACCESSION   DATLKL010000901 DATLKL010000000
VERSION     DATLKL010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33751"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VM713_12180"
     CDS             <1..490
                     /locus_tag="VM713_12180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVH85060.1"
                     /translation="APVEEALALLYAIVPENVEIDCVVHDDAPSISGDPSVVQQIVIN
                     LCTNAYQAMREAGGRITVRVSPASPPGAASVVCALLEVSDTGHGIDPALVPHIFEPFF
                     TTREVGEGTGLGLSVVHGLVDSLGGFITVESVLEQGTTFRVYFPIAADAANELRGIAH
                     GT"
     gene            501..899
                     /locus_tag="VM713_12185"
     CDS             501..899
                     /locus_tag="VM713_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVH85061.1"
                     /translation="MASILVVDDDVEVGEVLRRVLERGGFAVRVVNSAAAALAAVAAS
                     EPDVVITDVIMPRQNGVDLIGALRTQSPRIRIVAISGGGSFGPSSYKPDAISTHAYLA
                     AARQAGASEVLSKPFDMSDLLNAVRGVLTN"
     gene            complement(998..1396)
                     /locus_tag="VM713_12190"
     CDS             complement(998..1396)
                     /locus_tag="VM713_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85062.1"
                     /translation="MKIRHILLLCSAAAMPQLATADPAVTNPGELGEMRALVDFCTRI
                     DPQSSDSLRAEWHSLVGGQSQIEQNSAYKQQYAAFTSALARVPNDVGQAICTLTTEWW
                     KGTAGRGHGGDGDGSKDNPGKSKPRHEADR"
BASE COUNT          264 a          573 c          581 g          320 t
ORIGIN      
        1 cgccccggtc gaggaggcgc tcgcgctgct gtacgcgatc gttcctgaaa acgtggagat
       61 cgactgcgtc gtccacgacg acgcaccgag catcagcggc gatccgagcg tggtacagca
      121 gatcgtcatc aacctgtgca ccaacgccta ccaggccatg cgggaggcgg gcgggcgcat
      181 caccgtccgc gtcagccccg cctccccgcc cggagcggcg agcgtcgtct gcgcgctgct
      241 cgaggtcagc gatacggggc acgggatcga tccggccctc gtgccgcata tcttcgagcc
      301 cttcttcacg acgcgcgagg tgggtgaagg cacgggactc gggctttcgg tggtgcacgg
      361 cctcgtcgat tccctcggcg gcttcatcac cgtggagtcg gtgctcgagc agggcaccac
      421 tttcagagtc tatttcccca tcgccgcgga cgcggccaac gagctgcgcg gtatcgcgca
      481 cggcacttga gggtcaccac atggcgtcaa tcctggtggt cgacgatgac gttgaagtcg
      541 gcgaagtcct ccgccgggtc ctggagcgcg gcggattcgc cgtgcgcgtg gtcaactcgg
      601 cggccgcagc actcgcggcg gtcgccgcga gcgagcccga cgtagtcatc acggacgtca
      661 tcatgccgcg ccagaacggc gtggatctca tcggcgcatt gcgcacccag agcccgcgga
      721 ttcggatcgt cgccatctcc ggcggcggca gcttcggtcc ctcgagctac aagcccgatg
      781 cgatcagcac ccacgcctat ctcgccgccg cgcggcaggc gggtgcgagc gaggttcttt
      841 ccaagccctt cgatatgagc gacctgctga acgcagtgcg cggtgtgctg acgaattgat
      901 cttaggacgc ccgcctcagc gggtagcgac gctgtcttgc gcggcgcggt cgagctgctg
      961 ccggtagagc ttgagcagtt cctgagcgcg cgccgcgtta gcgatccgcc tcgtgccggg
     1021 gcttactctt gccgggattg tccttcgaac cgtcgccgtc gcccccgtga ccccgcccgg
     1081 cggtgccctt ccaccactct gtagtaagcg tgcagatggc ctggcccacg tcgttcggca
     1141 ctctcgcaag cgcgctcgtg aacgcagcgt attgctgttt gtaggcggaa ttctgctcga
     1201 tttgggactg accgccgacg aggctgtgcc attcggccct gagggagtcg gaactctgcg
     1261 ggtctattcg ggtgcagaag tcgaccagcg cgcgcatctc ccccaactcg cctgggttgg
     1321 ttacggcggg atccgcggtc gcgagttgtg gcatcgcagc cgcgctgcac aaaagaagga
     1381 tgtgacggat tttcatgttt tatgtctcga cactgcgcca gaattgcatg ctagcgggac
     1441 cgggcaccct cgacccggac tttgcgcaca tttccggaga actttgctcg cggccagcgc
     1501 tagggcgcgc cgagccgctc aacgcggggt cgatccggga tcgtgacgag ctgctgggcg
     1561 cagtgcggcg actgctgccg aattagcggg tggcgacgct gctctgcgtg gcgcggtcga
     1621 gctgctggcg gtagagcttc agcagctcgc tcggtgtgac gaagccctcg atgcgcaacc
     1681 atgactgccc gggcgccgcg cgcacgaacg tgatcgggct gtggctcatc ttgtcccc
//
LOCUS       DATLKL010000902         5070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_22275, whole genome shotgun sequence.
ACCESSION   DATLKL010000902 DATLKL010000000
VERSION     DATLKL010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22275"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VM713_12195"
     CDS             <1..337
                     /locus_tag="VM713_12195"
                     /inference="COORDINATES: protein motif:HMM:NF019883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85063.1"
                     /translation="SEEAGIKLLRELLDNLREQPAQISAQVIQRWAGREGGESLQKLL
                     EREEVITDAAAAAGELRAALVKLADLAAERRLQALEAKSRSGPLTADELQEFQRLISI
                     LSAREARRG"
     gene            524..2605
                     /gene="rpoD"
                     /locus_tag="VM713_12200"
     CDS             524..2605
                     /gene="rpoD"
                     /locus_tag="VM713_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HVH85064.1"
                     /translation="MKSAAAHKAVAAVASLRDKERGHAAALAARKGVTVARAPAPHGK
                     LNDSPRPGDARSAAGKLAGDKRAPAPERRVPVMPEDRQSQLKLLIARGKEQGYLTYAQ
                     VNDHLPSEIVDPEQIEDIVNTINDMGIPVYEKAPDTESLLASEPSAPADEEAIEEAAA
                     ALAALDAELGRTTDPVRMYMREMGTVELLTREGEIRIAKRIEEGLDAVRSALALYPAT
                     YDFLLRAYEPVKTGQARLVDVIVGFIDPNAPDVIAQPQNPTKVEIVAAEEKSDDDEEG
                     DEESEGEEEVVDTGPDPVEAAARFGSITKIYNQVLGSIAKLGAKDVKTLRQRKRLADQ
                     FMELRLSPRMFDALILNLRTHVAEVRQLEKEIMIIAVRDAGMPRKDFIASFPKNETSP
                     RWLARHVKGGKKYSAPLARFKDEIERRQKKLEQLEGVYHLSINDIKEVNREVSIGEAK
                     ARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFST
                     YATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREPTPEELAVRM
                     EMPEDKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTSVASPIDQATMESLRETTH
                     SVLAQLTPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSR
                     SEQLRSFLMDD"
     gene            complement(2674..2922)
                     /locus_tag="VM713_12205"
     CDS             complement(2674..2922)
                     /locus_tag="VM713_12205"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HVH85065.1"
                     /translation="MREGRLRELLARLREHQSAGSSLRAADRERLDTVSEAIGAPGAS
                     SAGALLRLERLAVRFEASHPALAETLREVIDTLGKGGL"
     gene            complement(2942..3148)
                     /locus_tag="VM713_12210"
     CDS             complement(2942..3148)
                     /locus_tag="VM713_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85066.1"
                     /translation="MATKLNKQLKREIEVDGKPYMITLSPEGVKLTEKGKRLGREHTW
                     KDLLGNGGGGAGGGAPGGAFGPAG"
     gene            complement(3157..3765)
                     /gene="gstA"
                     /locus_tag="VM713_12215"
     CDS             complement(3157..3765)
                     /gene="gstA"
                     /locus_tag="VM713_12215"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HVH85067.1"
                     /translation="MKLYYLTGACSLASVMSLREAGLKFELVKVDRKTKKAADGLDYL
                     EVNPKGYVPALTLDNGETLTENTAVLQYIADRNPAAKLAPAAGTMERYRLIEWLGFIN
                     SEIHKNFSPLFRADLHEAVKEYARTNLNARLDYAERALGSKSYLMGEQYTVADAYLYV
                     VLGWGVHVNFDIGRWPQLKRLHERVGARPHTLEALKSEGLAK"
     gene            4186..4255
                     /locus_tag="VM713_12220"
     tRNA            4186..4255
                     /locus_tag="VM713_12220"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4216..4218,aa:His,seq:gtg)
     gene            complement(4296..>5070)
                     /locus_tag="VM713_12225"
     CDS             complement(4296..>5070)
                     /locus_tag="VM713_12225"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HVH85068.1"
                     /translation="ARCRSGFKLGPQGRFATAAEGVRMKFPIPVCLVAGATLLWPAAQ
                     ADVPSRPLVVELFTAQGCSSCPPADAYLGQLSERPDVVALAFHVDYWDSSGWRDRFEL
                     RQAVERQNVYERNFHRSSAYTPQFVLDGRKDAIDTSSIVQALQEPRAAVPVTLAVREG
                     QVVIEVGEKQGERPSDVLLVAFLRHAVSNVGRGENAGKALEEFNIVRSVRTLGEWQGA
                     AVSYKVSASSLPADATDVAVLVQSRGEGPMVGAAAHSLR"
BASE COUNT          926 a         1703 c         1665 g          776 t
ORIGIN      
        1 ctcggaggag gcaggcatca agctgctgcg cgagctcctc gacaatctgc gcgagcagcc
       61 ggcgcagatc tccgcccagg tcatccagcg ctgggccggg cgcgagggcg gcgagtcgct
      121 gcagaaactc ctcgagcgcg aggaggtcat cacggatgcg gccgcggccg ccggagagct
      181 gcgcgccgcc ctcgtgaagc tcgccgatct cgccgccgag cggcgccttc aggccctcga
      241 agcgaagagt cggtctggtc cgttgaccgc tgatgaacta caggaatttc aaaggcttat
      301 atcgatcctc agcgcccgcg aggcacgccg cggctagcgg aatttggcgt ttgagccctt
      361 gtcaaataga attgcgcgct ttgccttggc gccccttgag ctgaccactt ttcggcagca
      421 cataggggaa cgaagcccat tgcgcccccc gggacacatg acacgcaagc acaagcctca
      481 gactgcacac aagaagcgcc cgtccgcacc aaccgccaag cccgtgaaat cggccgccgc
      541 ccacaaggcg gtcgcggcgg tcgcgagcct gcgcgacaag gagcgcggcc acgccgctgc
      601 gctcgccgcg cgcaagggcg tcaccgtggc gcgcgctccg gcgccgcatg gcaagttgaa
      661 cgacagtccg cgcccgggcg acgcccggag cgccgcgggc aagctggccg gtgacaagcg
      721 agcacccgcg cccgagcggc gcgtgcccgt catgccggag gaccggcagt cgcagctgaa
      781 gctgctgatc gcgcgcggca aggagcaggg ttacctcacc tacgcgcagg tcaacgacca
      841 cctcccgtcc gagatcgtcg atcccgagca gatcgaggac atcgtcaaca ccatcaacga
      901 catgggcatt ccggtgtacg agaaggcacc ggacaccgag tcgctgctgg cgagcgagcc
      961 ctccgctccc gcggacgagg aagcgatcga agaggcggcc gcggcgctcg ccgcgctcga
     1021 cgccgagctc ggccgcacca ccgatccggt gcgcatgtac atgcgcgaga tgggcaccgt
     1081 ggagctcctc acccgtgagg gcgagatccg catcgccaag cgcatcgagg aaggactcga
     1141 tgcggtgcgc agcgcgctcg cgctgtaccc ggcgacctat gacttcctgc tgcgtgccta
     1201 tgagccggtt aagaccggcc aggcgcgcct ggtcgacgtg atcgtgggct tcatcgatcc
     1261 gaatgcgccc gatgtgattg cgcagccgca gaatcccacc aaggtggaga tcgtcgcggc
     1321 cgaggaaaag tcggacgacg acgaagaggg cgacgaggag agcgagggcg aggaggaggt
     1381 cgtcgacacc ggtcccgatc cggtcgaggc ggcggcgcgc tttggcagca tcaccaagat
     1441 ctacaaccag gtgctcggct cgatcgccaa gctcggcgcc aaggacgtaa agacgctgcg
     1501 ccagcgcaag cgtctcgccg atcagttcat ggagctgcgg ctctcgccgc gcatgttcga
     1561 cgcgctgatc ctcaatctcc gcactcacgt ggcggaagtc cgccagctcg agaaggagat
     1621 catgatcatc gcggtgcgcg acgccggcat gccgcgcaag gacttcatcg cctcgttccc
     1681 caagaacgag accagcccgc gctggctggc gcgccacgtc aagggcggca agaagtactc
     1741 ggcgccgctg gcccggttca aggacgagat cgagcggcgg cagaagaagc tcgagcagct
     1801 cgagggcgtc tatcacctct cgatcaacga catcaaggaa gtcaaccgcg aggtctcgat
     1861 cggcgaggcc aaggcgcgcc gcgccaagaa ggagatggtc gaggccaacc tgcgtctggt
     1921 gatttccatc gccaagaagt acaccaaccg cgggctgcag ttcctcgatc tcatccagga
     1981 aggcaacatc ggcctcatga aggcggtcga caagttcgaa taccgccgtg gctacaagtt
     2041 cagcacctat gcgacctggt ggatccgcca ggcgatcacc cgctcgatcg ccgaccaggc
     2101 gcgcaccatc cgtatcccgg tgcacatgat cgagaccatc aacaagctga accgcatctc
     2161 gcggcagatg ctgcaggaga tgggccgcga gccgacgccc gaggagctgg cggtgcgcat
     2221 ggagatgccc gaggacaagg tgcgcaaggt cctcaagatc gccaaggagc cgatctcgat
     2281 ggagacgccc atcggcgacg acgaggattc gcacctcggg gacttcatcg aagacacctc
     2341 ggtggcctca ccgatcgacc aggccaccat ggagtcactg cgcgagacga cgcactcggt
     2401 gctggcgcag ctcaccccgc gcgaggccaa ggtgctgcgc atgcgcttcg gcatcgacat
     2461 gaataccgac cacaccctgg aggaggtcgg caagcagttc gacgtgacgc gcgagcgcat
     2521 ccggcagatc gaggccaagg cgctgcgcaa gctccgtcac ccgagccgca gcgagcagct
     2581 gcgcagcttc ctgatggacg actgagcagc acgccgcttg caggcagagt aaaggcccgg
     2641 tgtcgccaaa caccgggcct ttttactttg gggctagagt ccgcccttgc cgagggtgtc
     2701 gatgacctcg cgcaacgtct cggcaagcgc cgggtggctc gcctcgaatc ggaccgcgag
     2761 ccgctcgagg cgcagcaacg cgccggccga ggaggctccc ggcgcgccga tggcttcgct
     2821 caccgtgtcg agacgctcac ggtccgcggc gcgcagcgac gatccggcgc tctggtgctc
     2881 gcgcaggcgc gcgagcagct cacggagtct cccctcgcgc atcgcccccc cggcgagcgg
     2941 gttatcccgc gggtccgaac gcgccgcccg gcgcgccgcc gcccgcaccg ccaccgccgt
     3001 tgccgagcag atccttccag gtgtgctcgc ggccgaggcg cttgcccttc tcggtgagct
     3061 tcaccccctc gggggagagc gtgatcatgt agggcttgcc gtcgacctcg atctcgcgct
     3121 tgagctgctt gttgagcttg gtagccattg cgtcccctac ttcgccagac cttcggattt
     3181 gagagcctcg agcgtgtgcg gccgtgcgcc gacgcgctcg tgcagccgct tgagctgcgg
     3241 ccaccggcca atgtcgaaat tgacgtggac gccccagccc agcaccacgt agagataggc
     3301 atccgccacc gtgtactgct cgcccatcag atagctcttc gagcccaggg cacgctccgc
     3361 gtagtcgagg cgcgcgttga ggttggtgcg cgcgtattcc ttgaccgcct cgtgcaggtc
     3421 cgcccggaac aagggtgaga agttcttgtg tatttcggaa ttgatgaagc cgagccactc
     3481 gatgaggcga tagcgctcca tggtgcccgc ggccggcgcc agtttcgccg cgggattgcg
     3541 gtccgctatg tattgcagta cggcggtgtt ctcggtgagc gtctcgccgt tgtcgagcgt
     3601 gagcgccggc acatatccct tggggttcac ttccagataa tcgagcccgt cggccgcctt
     3661 cttggtcttg cggtccacct tgaccaactc gaacttcagt ccggcttcgc gcaacgacat
     3721 gaccgatgcc agcgagcagg cgccggtcag atagtagagc ttcatcactc ggtcctcctt
     3781 gtactccgcg ggcgccgccc gtggggcgcc tgcgcggaaa gcgttatgct aacgggccgc
     3841 gcaccgatgg caagaggatt ctcgtggcag gtcgcatttg atatggtccg tggtcgcagc
     3901 aggcgaagtc cgcggcaggc ggctgctaca atcggcgcgg ttgggcctgt agcacagcgg
     3961 ttagtgcagg ggactcatcg acacggtgcc ttcgcgcgga aacgcgcgaa gtggacggga
     4021 tgaattcagg ggaacctgcg gggactgaca cccatggcaa tcctgagccg agccgccggt
     4081 acaccggcgg aaggtgcaga gactacctga gggctcgagt gcccttgatg acaggcttga
     4141 gcatcccgca cccacacgcg cacttctgcg cacgggtgaa gagatagtcc actcccggtg
     4201 gaaacaccgg ggaaagtgaa tcccttggtc gtaggttcga atcctaccgg gcccagatca
     4261 gcgccgcgcg agcgatcgcg cggagtgtgg cggcttcagc gcagcgaatg agcggccgcg
     4321 cccaccatcg gcccctcgcc tcgcgactgc accagcaccg cgacatcggt tgcatccgct
     4381 ggcaacgaag acgcgctcac cttgtagctc accgccgcgc cttgccactc gcccagcgtg
     4441 cggacgctgc gcacgatgtt gaactcctcg agagccttgc cggcgttctc gccgcggccg
     4501 acgttggaca cggcatggcg caggaacgcc acgagcagga cgtcactcgg ccgctcgccc
     4561 tgtttctcgc cgacctcgat cacgacctga ccctcgcgca ccgcgagcgt cactggcacg
     4621 gcagcacgcg gctcctgcaa ggcctggacg atgctgctcg tgtcgatggc gtccttgcgt
     4681 ccgtcgagaa cgaactgtgg cgtgtaggca gaggagcggt ggaagttgcg ttcgtagacg
     4741 ttctggcgct cgaccgcctg gcggagctca aaccggtcgc gccatcctga gctgtcccag
     4801 tagtccacgt gaaacgccag cgccaccaca tcgggccgct cgctgagctg gccgaggtag
     4861 gcgtccgccg gagggcagga gctgcaccct tgcgcggtga agagctcgac caccagcggt
     4921 cgcgagggaa cgtcggcctg cgcggctggc cacagcagcg tcgcaccggc aaccaggcac
     4981 accggaatcg ggaatttcat ccgcacaccc tccgcggcgg tcgcgaacct gccttggggc
     5041 ccgagtttaa acccggagcg gcagcgggcc
//
LOCUS       DATLKL010000903         1982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_180336, whole genome shotgun sequence.
ACCESSION   DATLKL010000903 DATLKL010000000
VERSION     DATLKL010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180336"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..949)
                     /gene="xerD"
                     /locus_tag="VM713_12230"
     CDS             complement(44..949)
                     /gene="xerD"
                     /locus_tag="VM713_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HVH85069.1"
                     /translation="MTTHADSADPAVSRFLDAVWMERGLSPNTLAAYRADLTALARWL
                     AERSVPIMRTSRVDLQEFIASRVRGGARPRSTARQLSSFRRFFRYYMRESVIREDPTA
                     QIAMPKIGRSLPRSLTEEEVESLLAAPLVSDPLGNRDRTMLEVLYATGLRVSELVNLR
                     QGQINLNQGVIRILGKGDRERLIPLGEEAMRALKEFARGPRGEILLERQTDYLFPTRR
                     GDRMTRQAFWHIIKRYARKAGISKDLSPHTLRHAFATHLLNHGADLRVVQLLLGHSDL
                     STTQIYTHVARERMKELHSQHHPRG"
     gene            1214..>1982
                     /locus_tag="VM713_12235"
     CDS             1214..>1982
                     /locus_tag="VM713_12235"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVH85070.1"
                     /translation="MLLAPLKIAYALWVALAFLAIGLSALALMLAVPGMARRRAAGRR
                     AARIFLWLAAMPLTVKHEERLPAGQCVVVCNHASYLDGPVLTAALPPRFAFVIKREMS
                     AVPLAAIVLRRLGSVFVERNRTQASADARRLLRDASRGNSLVFFPEGTFDPRPGVRKF
                     HAGAFASAVRAGCPLVPAVLRGTRRAFWPGGSLPQPGPIELEILPPLTPSSGAAGDAV
                     PALREAARAAILAALGEPDLTCSDDTARPPDTAPVRSV"
BASE COUNT          274 a          685 c          705 g          318 t
ORIGIN      
        1 ggaccggcgg gacacgatgg ctcgaagtgc gcgccgcgcc gcgtcagccg cgcgggtggt
       61 gctgcgagtg cagctctttc atgcgctcgc gcgcgacgtg ggtgtagatc tgcgtcgtcg
      121 acagatcgct gtgtccgagc agcagctgca cgacgcgcag gtcggcgccg tgattcagca
      181 gatgcgtggc gaaggcatgg cgcagcgtgt ggggtgagag gtccttgctg atgccggcct
      241 tgcgcgcgta gcgcttgatg atgtgccaga aggcctggcg cgtcatgcgg tcgccgcgcc
      301 gcgtcgggaa caggtaatcg gtctggcgct cgagcaggat ctccccgcgc gggccgcgcg
      361 cgaattcctt cagcgcacgc atcgcctcct cgccgagcgg aatgagccgc tcgcggtcgc
      421 ccttgccgag gatgcggatg acgccctggt tgaggttgat ctgtccctgg cgcaggttca
      481 ccagctccga caccctcagg cccgtggcgt agagcacctc gagcatggtg cggtcgcggt
      541 taccgagcgg gtcactcacc agcggggcgg cgagcagtga ctcgacctcc tcctcggtga
      601 gcgagcgcgg cagcgagcgc ccgatcttgg gcatggcgat ctgcgcggta ggatcctcgc
      661 ggatcacgct ctcgcgcatg tagtagcgga agaagcgccg gaagctcgac agctgccgcg
      721 cggtcgagcg cggccgcgcc ccgccacgca cccgcgaggc gatgaactcc tgcaggtcga
      781 cgcgcgaggt gcgcatgatg ggaacgctgc gctcggcgag ccagcgggcg agcgcggtaa
      841 gatcggcgcg gtaggcggcg agcgtattcg gcgacaggcc gcgctccatc cagaccgcat
      901 ccaggaaacg agaaaccgcg ggatccgcgg aatcggcgtg agtggtcatg gagagagccg
      961 cagcctggtt tgacaagact tgtctcgctg gtcctcgggg cgcgcggcac gcgcagcatg
     1021 ccgtgtcaga gccccgactt ccgggtaatg tggtagacgg agtatggtga gcgcgacgga
     1081 tgcaccgacg tgatgacctt cacatctgtc ttccggcatt aacataaaga gaaccagttc
     1141 acacccaaag ttggcgcagc gtcccacccc catgaccgca cagcctccgc ggcggcccct
     1201 ccggctgcgc accttgctcc ttgccccgct caagattgcg tatgcgctgt gggtggcgct
     1261 ggccttcctc gcgatcgggc tgtcggcgct cgcgttgatg ctggcagtgc ccggaatggc
     1321 gcggcggcgc gcggcgggcc ggcgggccgc gcgcatcttc ctctggctcg ccgccatgcc
     1381 gctcaccgtg aagcacgagg agcggctccc cgcgggccaa tgcgtcgtgg tgtgcaacca
     1441 cgccagctac ctcgacgggc cggtgctgac ggcggcgctg ccgccgcgtt tcgcattcgt
     1501 catcaagcgc gagatgtccg ccgtgccgct cgccgccatc gtgctgcggc gcctcggctc
     1561 ggtgttcgtc gagcgtaacc gcacccaggc ctcggccgat gcccggcggc tgctgcgcga
     1621 cgcttcccgg ggcaattcgc tggtcttctt ccccgaaggc acgttcgacc cccggccagg
     1681 cgtgcgcaag ttccacgccg gggcgttcgc gagcgccgtg cgcgccggct gcccgctcgt
     1741 gccggcggtg ctgcgcggca cgcggcgggc cttctggccg ggtggctcgc tgccgcagcc
     1801 ggggccgatc gagctcgaga tcctcccgcc actcacgccc tcttccggcg ccgccgggga
     1861 tgcggtgccg gcgctgcgcg aggcggcgcg cgcggccatc ctcgccgcgc tcggggagcc
     1921 cgacctcaca tgttccgacg atactgcccg cccacctgat acagcgcccg tgagatctgt
     1981 cc
//
LOCUS       DATLKL010000904         2191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_33795, whole genome shotgun sequence.
ACCESSION   DATLKL010000904 DATLKL010000000
VERSION     DATLKL010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33795"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VM713_12240"
     CDS             <1..1275
                     /locus_tag="VM713_12240"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HVH85071.1"
                     /translation="ARIDAQRGSGAWQDMPLAGALHAETSELGLLSLYFPNIDRAAGE
                     LHADVNVAGTLGAPQLSGGVKLTGGELDLYQVNLGLRQVTLEAHLNDGGLDFSGNARA
                     GAGSAAANGHLEWRNLLPYGKFHLEGTNLRVADTPEAQIDASPDLAFAVDGRRIEVAG
                     TVKVPYAKIQPKDITNAVRTSPDEVIVGKPATSPAERFEVMSTVTLTLGDKVSVDVSG
                     LTGRLTGSVTIKSGYDAITRGTGELSVADGKYRAYARKLDIQRGRLIFTGGPIDDPGV
                     DLRAQKQFPDVTAGINVRGTLQQPRMSFFSDPPLPQSQIVSLILSGSAAQSTQSGSNE
                     AIGQAAALAAATVGSAVGLPDVSLETDPIANETSLVLGRYLTPRLYVSYGVSLTETLN
                     TLKLRYTLGDHWVVRTELGTARGADLVFSITK"
     gene            1393..1845
                     /locus_tag="VM713_12245"
     CDS             1393..1845
                     /locus_tag="VM713_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVH85072.1"
                     /translation="MLNDTEQLERRGAIVRILRAGLVRKQQDLVRLLKKAGHAATQSS
                     ISRDLRDLGVLKAGGGYVLPAEEPTRANGDFATLAQFVRELRRAGPAITVLRTTIGAA
                     QSVAVAIDRAEWPEVAGTISGDDTIFIATANSRAQDELHARLRALFRV"
     gene            1849..>2191
                     /locus_tag="VM713_12250"
     CDS             1849..>2191
                     /locus_tag="VM713_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HVH85073.1"
                     /translation="MSISVSSAPGAGTTPIVLAYSGGLDTSFLVPWIAETYRRPVITV
                     TVDTGGIDAETARALAERARALGALEHHQIDARADYFEQVLRFLIMGNVRRGQLYPLC
                     VGAERVMQAQTI"
BASE COUNT          343 a          812 c          718 g          318 t
ORIGIN      
        1 gcccgcatcg atgcgcagcg cggcagcggt gcatggcagg acatgccgct cgccggcgcg
       61 ctgcacgccg aaacctccga gctcgggttg ctgtcgctct atttccccaa catcgatcgc
      121 gccgcgggcg agctgcacgc ggacgtcaac gtggccggca cgctcggcgc cccgcagctg
      181 agcggcggcg tcaagctgac gggtggcgag ctcgatctct atcaggtgaa tctcgggctg
      241 cgccaggtga cgctcgaggc acacctcaac gacggcggcc tcgacttcag cggcaacgcc
      301 cgcgccggcg ccggcagcgc cgccgccaac ggccacctcg agtggcgtaa cctcctgccg
      361 tacggcaagt tccacctcga gggcacgaat ctgcgcgtgg cggatacccc cgaggcccag
      421 atcgacgcct cgcccgacct cgccttcgcg gtcgacgggc ggcgcatcga ggtcgccggt
      481 accgtcaagg tgccgtacgc gaagatccag ccgaaggaca tcaccaacgc ggtacgcacc
      541 tcgcccgacg aggtgatcgt cggcaagccg gcgacgagcc cggcggagcg tttcgaggtg
      601 atgagcacgg tcacgctgac cctcggcgac aaggtctcgg tcgacgtctc ggggctcacc
      661 ggtcgcctca ccggcagcgt caccatcaag agcggctacg acgcgatcac gcgtggcacc
      721 ggggagctct cggtcgccga cggcaagtac cgcgcctacg cgcgcaagct cgacatccag
      781 cgcggccggc tcatcttcac tggcggcccg atcgacgacc ccggcgtcga cctgcgggca
      841 cagaagcaat tcccggacgt gaccgcgggg atcaacgtgc gcggcaccct gcagcagccg
      901 cgcatgtcgt tcttctccga tccgccgctg ccgcagtcgc agatcgtatc gctgattctc
      961 tccggctccg ccgcccagtc gacgcagagt ggcagcaacg aggccatcgg gcaggccgcg
     1021 gcgctcgccg cggcgacggt tggctcggcc gtcgggctcc ccgacgtgag cctcgaaacc
     1081 gacccgatcg ccaacgagac ctcactggtg ctcgggcgct atctcacgcc acgcctctac
     1141 gtgagttatg gcgtgagcct caccgagacg ctcaatacgc tcaagctccg ttacacactc
     1201 ggcgaccact gggtggtacg caccgagctc ggcaccgcgc gcggcgcgga cctggtcttc
     1261 agcatcacca agtaggcggc ggccgccgcg acccaggcgc accgctgcct ggctccccgg
     1321 ccgcacgcgc gcttgacgcc tgtgcattaa tatgcagaat gtgcgcatct ttatgcacat
     1381 ggatctccag ccgtgctgaa cgataccgag cagctcgagc gccgcggcgc catcgtgcgc
     1441 atcctgcgcg ccggcctggt gcgcaagcag caggacctgg tgcggctgct gaagaaggca
     1501 gggcacgcgg ccactcagtc ctccatcagc cgcgacctgc gcgacctcgg cgtgctcaag
     1561 gcgggcggcg gctacgtgct gccggcggag gagccgacac gtgccaacgg ggatttcgcc
     1621 acgctcgcgc agttcgtgcg cgagctgcgg cgcgcgggtc ccgcgatcac ggtgctgcgt
     1681 accacgatcg gcgccgcgca gtcggttgcc gtggccatcg accgggccga gtggcccgag
     1741 gtcgccggca cgatctccgg cgacgacacc atcttcatcg cgaccgccaa ctcgcgcgcg
     1801 caggacgagc tgcacgcccg cctgcgcgcc ctcttcaggg tctgatccat gtccataagc
     1861 gtcagcagcg ccccgggcgc gggcaccact cccatcgtcc tcgcctactc cggcggtctc
     1921 gacacctcgt tcctggtgcc gtggatcgcc gagacctacc gccgcccggt gattaccgtg
     1981 acggtcgata ccggtggcat cgacgccgag acggcccgcg cgctcgctga gcgggcgcgg
     2041 gcgctcgggg ccctcgagca tcaccagatc gatgcgcgcg ccgactattt cgagcaggtg
     2101 ctgcgcttcc tcatcatggg caacgtgcgc cgcggccagc tctacccgct gtgcgtcggc
     2161 gccgagcgcg tcatgcaggc gcagaccatc g
//
LOCUS       DATLKL010000905         1521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_56353, whole genome shotgun sequence.
ACCESSION   DATLKL010000905 DATLKL010000000
VERSION     DATLKL010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56353"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..>1521
                     /gene="trkA"
                     /locus_tag="VM713_12255"
     CDS             193..>1521
                     /gene="trkA"
                     /locus_tag="VM713_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HVH85074.1"
                     /translation="MKILILGAGQVGRTAASALSREEANEVTVVDLNEEALRDLQERL
                     EVRTVVGNAAHPTVLEAAGAGEADVMVALTSSDEVNLMACEVAFALFRTPTKIARIRA
                     AEYTDRAELFGVDALSVDVLISPERLVTEYVERLIHHPGTLQVLDFADGKVRLVAVRA
                     RRGGPLVGHALRELPEHLPKMAVRVVAIYRDGRSVPPEGDTVIAEGDEVFFLATREDI
                     DRVTSEMRPGDQAVRRVVIAGAGNIGFRLAKTLEHSNQVKLIERDARRARKVSELLDR
                     AIVLHGDAADEELLIEENIDSADVFAALTNSEEANILSAMLARRLGAAKVMALINKPS
                     YTELVESGSIDIAVSPQTITIGSLLAHVRRGDVVRVHALRRGAAEAIEAIVHGDERSS
                     RVVGRTVCEIPLPEGAAIGAVVRGDAVIMAHHDTVVRADDHVILFLADRRH"
BASE COUNT          231 a          520 c          544 g          226 t
ORIGIN      
        1 aacgtcgagg ggaacggtgc gcatgcgcgc gtgcaggtga gcttcgagcg gcagggcacc
       61 aagtggctga tggttcagta tgcgaacctc gagcctttgt aatttggcaa aactggcctg
      121 cggccgtttt gcggaccgtg ccttcgttgc cggcggcaaa aggcgcgact tttgccgcct
      181 gcgtaggcgc gcatgaagat cctcatcctc ggcgccggtc aggtcgggcg gaccgccgcc
      241 tcggccctct cgcgcgagga ggcgaacgag gtcaccgtgg tcgacctcaa cgaggaggcg
      301 ctgcgcgacc tgcaggagcg gctcgaggtg cgcaccgtgg tcggcaacgc cgcccacccg
      361 accgtgctcg aggcggccgg cgccggcgag gccgacgtca tggttgcgct cacctcgagc
      421 gatgaggtga acctcatggc gtgcgaggtg gcgttcgcgc tgtttcgtac gccgaccaag
      481 atcgcgcgca tccgcgccgc cgagtacacc gaccgcgcgg agctcttcgg tgtggacgcg
      541 ctctcggtcg atgtgctcat cagtcccgag cgcctcgtca ccgaatacgt cgagcgcctc
      601 atccaccacc ccgggacgct ccaggtgctc gacttcgccg atggcaaggt gcgcctggtc
      661 gcggtgcgcg cccggcgcgg cggaccgctg gtcgggcacg cgctgcgcga gctccccgag
      721 cacctgccga agatggcggt gcgggtggtg gcgatctacc gcgacgggcg cagcgtgccg
      781 cccgagggtg acacggtgat cgccgagggg gacgaggtgt tcttcctggc gacgcgcgag
      841 gacatcgacc gcgtcaccag cgagatgcgc cccggggacc aggcggtacg gcgcgtggtg
      901 atcgcgggcg ccggcaacat cggcttccgc ctggcgaaga ccctcgagca ctccaaccag
      961 gtgaaactca tcgagcgcga cgcgcggcgc gcccgcaagg tttccgagct cctcgaccgg
     1021 gcgatcgtgc tgcacggcga cgccgccgac gaggagctgc tcatcgagga gaacatcgac
     1081 agcgccgacg tgttcgcggc tctcaccaac tccgaggagg ccaacattct ctcggcgatg
     1141 ctggcgcggc ggctcggcgc ggccaaggtg atggcgctca tcaacaagcc ctcctacacc
     1201 gagctggtcg agtcgggcag catcgacatc gccgtctcgc cgcagaccat caccatcggc
     1261 tcgctgctcg cgcacgtgcg ccgcggcgac gtcgtgcgcg tgcacgcgct gcggcgcggc
     1321 gcggccgagg ccatcgaggc gatcgtgcac ggcgatgagc gcagctcgcg ggtggtcgga
     1381 cgcacggtgt gcgagatccc gcttcccgag ggcgccgcga tcggcgcggt ggtgcgcggc
     1441 gatgccgtca tcatggcgca ccatgacacg gtggtgcgcg ccgacgacca cgtaatcctc
     1501 ttcctggccg atcgacgcca c
//
LOCUS       DATLKL010000906         2151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U13721
            k141_11257, whole genome shotgun sequence.
ACCESSION   DATLKL010000906 DATLKL010000000
VERSION     DATLKL010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939599
            Sequence Read Archive: SRR8500456
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:11:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,453
            CDSs (total)                      :: 2,436
            Genes (coding)                    :: 2,403
            CDSs (with protein)               :: 2,403
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 7 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 9 of 33
            Pseudo Genes (multiple problems)  :: 2 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11257"
                     /isolate="SMAG_U13721"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="22.99 N 108.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /gene="fahA"
                     /locus_tag="VM713_12260"
     CDS             complement(<1..1156)
                     /gene="fahA"
                     /locus_tag="VM713_12260"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HVH85075.1"
                     /translation="MAGVDATHDPQLTSWVASANDPGSDFPIQNLPFAAFRRAGSREP
                     FRGGVAIGAEILDLGALAAAAPFGGVAREALAAAAHPSLNALMGSGPAASAALRGALS
                     EALRTGSPLAARLQTALVPQSAAEYRVAADVGDYTDFYASIHHATSVGRLFRPDQPLL
                     PNYKWVPIAYHGRASSLRVSGYDIARPMGQVLAADAAAPELAATRRLDYELEVGAFIG
                     RGNELGTSIPLAAAEQHVFGLCLLNDWSARDIQSWEYQPLGPFLAKNFATTVSPWVVT
                     LEALAPFRTPWTRPPGEPPPLSYLEGAELRRAGGFNVELEVWLETARMRAAGLAAQRL
                     SHSNFRDSWWTVSQMVTHHTVNGCNLEPGDLLGSGTQSGATPGEAGSLLEL"
     gene            complement(1177..>2151)
                     /locus_tag="VM713_12265"
     CDS             complement(1177..>2151)
                     /locus_tag="VM713_12265"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HVH85076.1"
                     /translation="ANRSMRERFFCDADGELLIVPQLGALRFLTELGCLEAEPQEIVV
                     LPRGLRFAVELLGAEARGYICENFGAPFRLPDLGPIGSNGLANPRDFATPVAWYEDRE
                     GDFELVEKFAGQLWAARIGHSPLDVVAWHGNLAPYKYDLRRFNTLGSVSYDHPDPSIF
                     LVLQSPSDTPGVDTIDFVIFPPRWLVMQDTFRPPWFHRNVASEFMGLIAGTYDAKAEG
                     FTPGGASLHNCMSGHGPDAATWEKAVSADTSRPVHIANTMAFMFETRAVIRPTRFALN
                     SPQLQGDYQRVWRDLPKSFSPDAPSVRPPRRAGATGGASPATGKRRRG"
BASE COUNT          338 a          725 c          803 g          285 t
ORIGIN      
        1 tcagctcgag gagcgagccg gcctcgccgg gcgtcgctcc cgactgcgtg ccgctgccga
       61 ggaggtcgcc gggctcgagg ttgcagccgt tgaccgtgtg gtgggtcacc atctgcgaca
      121 ccgtccacca ggaatcgcgg aagttcgagt gcgagaggcg ctgcgccgcg agccccgcgg
      181 cgcgcatgcg cgccgtctcg agccacactt cgagctcgac gttgaagccg cccgcgcgcc
      241 gcagctccgc gccctcgagg tatgaaaggg gcggcggctc gcccggcggc cttgtccagg
      301 gagtgcgaaa gggcgcgagc gcctcgagcg tcaccaccca cggcgagacc gtggtcgcga
      361 agttcttggc gagaaacggc ccgagcggct ggtactccca cgactggatg tcgcgggccg
      421 accaatcatt gagcaggcag agcccgaaga cgtgctgctc ggccgccgcg agcggaatag
      481 acgtgccgag ctcgttgccg cggccgatga aggctcccac ctcgagctcg taatcgagcc
      541 ggcgcgtcgc ggcgagctcg ggtgccgccg catccgcggc cagcacctga cccatcggcc
      601 gggcgatgtc gtagcccgag acgcgcagcg aggaggcgcg gccatggtag gcgatcggca
      661 cccatttgta gttcggcagc agcggctggt ccggacgaaa cagccgtccg acggaggtgg
      721 cgtgatggat cgaggcgtaa aagtcggtgt agtcgccgac gtcggcggcg acacggtact
      781 cggccgcgct ctgcggcacg agcgccgtct gcagccgcgc ggcgagcggg ctgcccgtgc
      841 gcagcgcctc cgagagcgcg ccgcgcagcg cggcgctggc ggcagggcct gagcccatca
      901 gcgcgttcag cgacgggtgc gcggccgcgg cgagcgcctc gcgcgcaacg ccgccgaaag
      961 gcgccgcggc cgcgagcgca ccgaggtcga ggatctccgc gccgatcgcg acgccgccgc
     1021 ggaagggctc gcggctaccg gcgcggcgaa aagccgcgaa cggcagattc tggatgggaa
     1081 agtcgctccc cgggtcgttg gcggaggcca cccagctcgt gagctgcggg tcgtgggtcg
     1141 cgtcgacgcc ggccatgccg ctagtgtgcc agcccgtcag ccacggcgcc gcttgccggt
     1201 cgcggggctc gctccgcccg tcgcgccggc tcgacggggc gggcggacag agggggcgtc
     1261 gggcgagaag ctcttcggta aatcccgcca gacacgctgg tagtcaccct ggagctgcgg
     1321 cgagttgagg gcaaagcgtg tcggacgaat caccgcgcgc gtctcgaaca tgaaggccat
     1381 ggtgttggcg atgtgcaccg ggcgcgaggt gtcggcgctc accgccttct cccaggtggc
     1441 cgcatccggc ccgtgcccgc tcatgcagtt gtggagcgag gcgccgcccg gggtgaagcc
     1501 ctcagccttg gcgtcgtagg tgccggcgat gagacccatg aactcgctcg ccacgttgcg
     1561 gtgaaaccac ggcggccgga acgtgtcctg catgacgagc cagcggggcg gaaagatcac
     1621 gaagtcgatg gtgtcgaccc ccggcgtgtc gctcggcgac tgcaggacga ggaaaatcga
     1681 cgggtcgggg tggtcgtagc tcactgagcc gagcgtgttg aagcggcgca ggtcgtactt
     1741 gtagggggcg aggttcccgt gccacgccac cacatcgagc ggcgaatgac cgatgcgcgc
     1801 cgcccacagc tgcccggcaa acttctcgac cagctcgaag tcgccttcgc ggtcctcgta
     1861 ccaggcgacc ggcgtcgcga agtcgcgcgg attggcgagc ccgttcgagc cgatggggcc
     1921 caagtcgggc agacggaacg gtgccccgaa gttctcgcag atgtaaccgc gcgcctcggc
     1981 gccgaggagc tccaccgcga agcgcagccc gcgcggcaga acgacgatct cctgcggctc
     2041 ggcctcgaga cagccgagct cggtcaggaa gcgcagcgcg ccgagctgcg ggacgatgag
     2101 gagctccccg tccgcgtcgc agaagaagcg ctcgcgcatc gagcggttgg c
//
